LOCUS       DAVLRT010000001        73643 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLRT010000001 DAVLRT010000000
VERSION     DAVLRT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 73643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.001"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            20..532
                     /locus_tag="VXM85_000001"
     CDS             20..532
                     /locus_tag="VXM85_000001"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1344662.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            714..1658
                     /locus_tag="VXM85_000002"
     CDS             714..1658
                     /locus_tag="VXM85_000002"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1344663.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            1667..2344
                     /locus_tag="VXM85_000003"
     CDS             1667..2344
                     /locus_tag="VXM85_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1344664.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            2442..3200
                     /locus_tag="VXM85_000004"
     CDS             2442..3200
                     /locus_tag="VXM85_000004"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1344665.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            3238..4743
                     /gene="lytC"
                     /locus_tag="VXM85_000005"
     CDS             3238..4743
                     /gene="lytC"
                     /locus_tag="VXM85_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1344666.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRNGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            5239..5757
                     /locus_tag="VXM85_000006"
     CDS             5239..5757
                     /locus_tag="VXM85_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1344667.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            5887..6393
                     /locus_tag="VXM85_000007"
     CDS             5887..6393
                     /locus_tag="VXM85_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1344668.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            6393..6563
                     /locus_tag="VXM85_000008"
     CDS             6393..6563
                     /locus_tag="VXM85_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344669.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            6595..7827
                     /gene="clpX"
                     /locus_tag="VXM85_000009"
     CDS             6595..7827
                     /gene="clpX"
                     /locus_tag="VXM85_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1344670.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            7836..8423
                     /locus_tag="VXM85_000010"
     CDS             7836..8423
                     /locus_tag="VXM85_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1344671.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            8434..8814
                     /locus_tag="VXM85_000011"
     CDS             8434..8814
                     /locus_tag="VXM85_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1344672.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            8866..9756
                     /gene="rapZ"
                     /locus_tag="VXM85_000012"
     CDS             8866..9756
                     /gene="rapZ"
                     /locus_tag="VXM85_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1344673.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            9753..10730
                     /locus_tag="VXM85_000013"
     CDS             9753..10730
                     /locus_tag="VXM85_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1344674.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDFRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            10727..11638
                     /gene="whiA"
                     /locus_tag="VXM85_000014"
     CDS             10727..11638
                     /gene="whiA"
                     /locus_tag="VXM85_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1344675.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(11672..12640)
                     /locus_tag="VXM85_000015"
     CDS             complement(11672..12640)
                     /locus_tag="VXM85_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1344676.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELESVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            12776..13633
                     /locus_tag="VXM85_000016"
     CDS             12776..13633
                     /locus_tag="VXM85_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1344677.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            13620..14399
                     /locus_tag="VXM85_000017"
     CDS             13620..14399
                     /locus_tag="VXM85_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1344678.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            14423..15775
                     /locus_tag="VXM85_000018"
     CDS             14423..15775
                     /locus_tag="VXM85_000018"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1344679.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGNIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            15849..16223
                     /locus_tag="VXM85_000019"
     CDS             15849..16223
                     /locus_tag="VXM85_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1344680.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            16225..17073
                     /locus_tag="VXM85_000020"
     CDS             16225..17073
                     /locus_tag="VXM85_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1344681.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            17350..18117
                     /locus_tag="VXM85_000021"
     CDS             17350..18117
                     /locus_tag="VXM85_000021"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1344682.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMHIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            18129..19313
                     /locus_tag="VXM85_000022"
     CDS             18129..19313
                     /locus_tag="VXM85_000022"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1344683.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHNPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            19310..21181
                     /locus_tag="VXM85_000023"
     CDS             19310..21181
                     /locus_tag="VXM85_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1344684.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(21324..22508)
                     /gene="mntE"
                     /locus_tag="VXM85_000024"
     CDS             complement(21324..22508)
                     /gene="mntE"
                     /locus_tag="VXM85_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1344685.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLATGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            22812..25508
                     /locus_tag="VXM85_000025"
     CDS             22812..25508
                     /locus_tag="VXM85_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1344686.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVDLATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            25521..26312
                     /gene="yghU"
                     /locus_tag="VXM85_000026"
     CDS             25521..26312
                     /gene="yghU"
                     /locus_tag="VXM85_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1344687.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRQTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QENLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            26428..26838
                     /locus_tag="VXM85_000027"
     CDS             26428..26838
                     /locus_tag="VXM85_000027"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1344688.1"
                     /translation="MEKKIVKDNLFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            26850..28445
                     /locus_tag="VXM85_000028"
     CDS             26850..28445
                     /locus_tag="VXM85_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344689.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            28612..28776
                     /locus_tag="VXM85_000029"
     CDS             28612..28776
                     /locus_tag="VXM85_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1344690.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            28855..29583
                     /gene="yaaA"
                     /locus_tag="VXM85_000030"
     CDS             28855..29583
                     /gene="yaaA"
                     /locus_tag="VXM85_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1344691.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(29861..30409)
                     /locus_tag="VXM85_000031"
     CDS             complement(29861..30409)
                     /locus_tag="VXM85_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1344692.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALASKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            30730..31239
                     /locus_tag="VXM85_000032"
     CDS             30730..31239
                     /locus_tag="VXM85_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1344693.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GLWNYPTVF"
     gene            31215..32402
                     /locus_tag="VXM85_000033"
     CDS             31215..32402
                     /locus_tag="VXM85_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1344694.1"
                     /translation="MELSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            32395..32760
                     /locus_tag="VXM85_000034"
     CDS             32395..32760
                     /locus_tag="VXM85_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1344695.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            32776..34122
                     /gene="asnS"
                     /locus_tag="VXM85_000035"
     CDS             32776..34122
                     /gene="asnS"
                     /locus_tag="VXM85_000035"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1344696.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            34275..34565
                     /locus_tag="VXM85_000036"
     CDS             34275..34565
                     /locus_tag="VXM85_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1344697.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            34577..35047
                     /gene="ssbA"
                     /locus_tag="VXM85_000037"
     CDS             34577..35047
                     /gene="ssbA"
                     /locus_tag="VXM85_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1344698.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            35079..35318
                     /locus_tag="VXM85_000038"
     CDS             35079..35318
                     /locus_tag="VXM85_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1344699.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            35613..37013
                     /locus_tag="VXM85_000039"
     CDS             35613..37013
                     /locus_tag="VXM85_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1344700.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            37015..37374
                     /locus_tag="VXM85_000040"
     CDS             37015..37374
                     /locus_tag="VXM85_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1344701.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            37428..38177
                     /locus_tag="VXM85_000041"
     CDS             37428..38177
                     /locus_tag="VXM85_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1344702.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            38167..38466
                     /locus_tag="VXM85_000042"
     CDS             38167..38466
                     /locus_tag="VXM85_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1344703.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            38537..39472
                     /locus_tag="VXM85_000043"
     CDS             38537..39472
                     /locus_tag="VXM85_000043"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1344704.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            39558..40070
                     /locus_tag="VXM85_000044"
     CDS             39558..40070
                     /locus_tag="VXM85_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1344705.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            40095..40703
                     /locus_tag="VXM85_000045"
     CDS             40095..40703
                     /locus_tag="VXM85_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1344706.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            40804..41001
                     /locus_tag="VXM85_000046"
     CDS             40804..41001
                     /locus_tag="VXM85_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1344707.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(41044..42489)
                     /locus_tag="VXM85_000047"
     CDS             complement(41044..42489)
                     /locus_tag="VXM85_000047"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1344708.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            42595..44217
                     /locus_tag="VXM85_000048"
     CDS             42595..44217
                     /locus_tag="VXM85_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1344709.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEDSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            44420..46378
                     /locus_tag="VXM85_000049"
     CDS             44420..46378
                     /locus_tag="VXM85_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1344710.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYRGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            46550..47095
                     /locus_tag="VXM85_000050"
     CDS             46550..47095
                     /locus_tag="VXM85_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344711.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDMSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEI"
     gene            47104..47643
                     /locus_tag="VXM85_000051"
     CDS             47104..47643
                     /locus_tag="VXM85_000051"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344712.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETSFALLFIFATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKLLLLDHLDQENQRLCVC"
     gene            47679..48224
                     /locus_tag="VXM85_000052"
     CDS             47679..48224
                     /locus_tag="VXM85_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344713.1"
                     /translation="MEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLV
                     TDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEILI
                     FDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSGT
                     HKELLENHERYARFVQEQMIE"
     gene            48381..49475
                     /locus_tag="VXM85_000053"
     CDS             48381..49475
                     /locus_tag="VXM85_000053"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1344714.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            49483..50649
                     /locus_tag="VXM85_000054"
     CDS             49483..50649
                     /locus_tag="VXM85_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1344715.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            50742..53840
                     /locus_tag="VXM85_000055"
     CDS             50742..53840
                     /locus_tag="VXM85_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1344716.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGLTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            53888..54505
                     /locus_tag="VXM85_000056"
     CDS             53888..54505
                     /locus_tag="VXM85_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1344717.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            54516..55850
                     /locus_tag="VXM85_000057"
     CDS             54516..55850
                     /locus_tag="VXM85_000057"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1344718.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            55860..56324
                     /locus_tag="VXM85_000058"
     CDS             55860..56324
                     /locus_tag="VXM85_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1344719.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEEILLYATSQGYSMIWFNPFYLEEQ"
     gene            56321..56815
                     /locus_tag="VXM85_000059"
     CDS             56321..56815
                     /locus_tag="VXM85_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1344720.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            56896..58551
                     /gene="mltG"
                     /locus_tag="VXM85_000060"
     CDS             56896..58551
                     /gene="mltG"
                     /locus_tag="VXM85_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1344721.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            58622..59104
                     /gene="greA"
                     /locus_tag="VXM85_000061"
     CDS             58622..59104
                     /gene="greA"
                     /locus_tag="VXM85_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1344722.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            59432..59830
                     /locus_tag="VXM85_000062"
     CDS             59432..59830
                     /locus_tag="VXM85_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1344723.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            60275..61282
                     /locus_tag="VXM85_000063"
                     /pseudo
     CDS             60275..61282
                     /locus_tag="VXM85_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            61357..61557
                     /locus_tag="VXM85_000064"
     CDS             61357..61557
                     /locus_tag="VXM85_000064"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1344724.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            61592..62308
                     /gene="atpB"
                     /locus_tag="VXM85_000065"
     CDS             61592..62308
                     /gene="atpB"
                     /locus_tag="VXM85_000065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1344725.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQTIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            62322..62816
                     /gene="atpF"
                     /locus_tag="VXM85_000066"
     CDS             62322..62816
                     /gene="atpF"
                     /locus_tag="VXM85_000066"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1344726.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            62816..63352
                     /locus_tag="VXM85_000067"
     CDS             62816..63352
                     /locus_tag="VXM85_000067"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1344727.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            63367..64872
                     /locus_tag="VXM85_000068"
     CDS             63367..64872
                     /locus_tag="VXM85_000068"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1344728.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            64888..65766
                     /locus_tag="VXM85_000069"
     CDS             64888..65766
                     /locus_tag="VXM85_000069"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1344729.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            65852..67258
                     /gene="atpD"
                     /locus_tag="VXM85_000070"
     CDS             65852..67258
                     /gene="atpD"
                     /locus_tag="VXM85_000070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1344730.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            67269..67688
                     /locus_tag="VXM85_000071"
     CDS             67269..67688
                     /locus_tag="VXM85_000071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1344731.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(67733..68746)
                     /locus_tag="VXM85_000072"
     CDS             complement(67733..68746)
                     /locus_tag="VXM85_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1344732.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            68890..70056
                     /locus_tag="VXM85_000073"
     CDS             68890..70056
                     /locus_tag="VXM85_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1344733.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            70046..71275
                     /locus_tag="VXM85_000074"
     CDS             70046..71275
                     /locus_tag="VXM85_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1344734.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            71421..72062
                     /locus_tag="VXM85_000075"
     CDS             71421..72062
                     /locus_tag="VXM85_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1344735.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGVVTIISKKVEKKYSYYR"
     gene            72072..72701
                     /locus_tag="VXM85_000076"
     CDS             72072..72701
                     /locus_tag="VXM85_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1344736.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASELLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            72714..73550
                     /locus_tag="VXM85_000077"
     CDS             72714..73550
                     /locus_tag="VXM85_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1344737.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
BASE COUNT        21819 a        13675 c        16744 g        21405 t
ORIGIN      
        1 tttgaaagaa agaaaaaaga tgaatttaaa agattacatt gcaacaattg aaaattatcc
       61 aaaggaaggc attaccttcc gtgatattag tcctttgatg gctgatggaa atgcttatag
      121 ctacgctgtt cgtgaaatcg ttcagtatgc tactgacaag aaagtcgaca tgatcgtggg
      181 acctgaagct cgtggattta tcgtgggttg tccagttgcc tttgagttgg gaattggttt
      241 tgcgcctgtt cgtaagccag gtaaattgcc acgtgaagtt atttctgctg actatgaaaa
      301 agagtacggt gtcgatacct tgactatgca cgcggatgcc attaagccag gtcaacgtgt
      361 tcttattgta gatgaccttt tggcgacagg tggaactgtt aaggcaacta tcgagatgat
      421 tgaaaaactt ggtggtgtta tggcaggttg tgccttcctt gttgaattgg atgaattgaa
      481 cggccgtgaa aaaattggtg actacgacta caaagttctt atgcattatt aatgaaaaca
      541 gtccctaggg ctgttttctc tacactagga tataaaaata gactataact agttagagaa
      601 aaactataat tgaaaactat atcttcttgc agtataataa aaggactaag tgtttgagat
      661 ttgtcttcaa acatatgcaa ttattcctga aagagtacag ttaggagagg gttatgccga
      721 ttcgaattga taaaaaattg ccagctgttg agattttacg gacagagaat atctttgtca
      781 tggatgatca acgtgctgcc caccaagata tccgtccttt gaagatttta attttaaatc
      841 tcatgccaca gaaaatggtc acagagaccc agttgttgcg ccacttggct aatacacccc
      901 tacaactgga tattgatttt ctctatatgg agagccaccg ttctaaaaca actcgttcag
      961 agcacatgga gaccttctat aaaacttttc ctgaagtcaa ggatgagtat tttgatggga
     1021 tgatcatcac gggtgctcca gttgagcatt taccatttga ggaagtggac tattgggagg
     1081 aatttagaca gatgcttgag tggtctaaga ctcatgtcta ttcgaccctt catatctgtt
     1141 ggggggctca ggctgggctt tatctgcgct atggtgtaga aaaataccag atggacagta
     1201 agctatcagg tatttatcct caggacaccc taaaagaggg tcaccttcta tttagaggct
     1261 ttgatgatag ctatgtatcc cctcattcac ggcacacgga gatttctaag gaagaggtct
     1321 taaacaagac caatctcgag attttatcag aaggacctca ggttggggtt tctattttgg
     1381 ccagtcgtga tttacgagaa atttatagtt ttggtcattt ggagtatgac cgtgatacct
     1441 tggcaaaaga gtattttcga gatcgtgatg caggtttcga cccacatatt ccagaaaatt
     1501 actttaaaga tgatgatgtt aatcaggtac cttgtctttg ttggtcttca tctgcagccc
     1561 tctttttcag taattgggta aaccatgcgg tctatcagga gacgcctttt gattggagaa
     1621 agatagaaga tgatgcatct gcatatgggt atttataaga ggaattatga catatttaga
     1681 cgcttttaaa tcaggtacct tggttttacc gagtgccctg ctcttgcatt ttaaggaact
     1741 ctttccttct agcgacgatt ttctggtttg gcaatttttc tatttgcaaa atacgacagg
     1801 cttagaagaa atgtcgccaa gccagattgc tgaaaggatt ggcaaggaaa tttcggatgt
     1861 caaccagtcc atttctaatc tgacggaaag gggactgctc cagtatcgta ctatcgaatt
     1921 aaatggcgaa attgaattgc tctttgatgc tagtttggcc ttggaacgtt tggatgacct
     1981 gtttggagca gttcattcaa gttcagacca gctaacacct caaaaccagc tcaaggattt
     2041 ggtggaaacc ttccagcagg agttgggacg attgttgacg ccttttgaga ttgaggattt
     2101 gaccaagaca ctaaaggaag atggaaccag tgctgacttg attaaggagg ctcttcgtga
     2161 agctgttttg aatggaaaac caaactggaa gtacattcag gctattttga gaaactggcg
     2221 ccatgaagga atcaagagtg tggctcaaat tgaggccaag agagcagaaa gagaagcaag
     2281 caatcctcag ttgacacagg tatctgcaga tttcagaaat gccatggatc tctggaagga
     2341 ttaatccatg caagtaggct tgaaatccga gtaagatttg caagctgtgt ataattgtga
     2401 tagaataaat agaaaataaa ttgaaaaaag aggtatgtga aatgtcacgt aaaccattta
     2461 tcgctggtaa ctggaaaatg aacaaaaatc cagaagaagc taaagcattc gttgaagcag
     2521 ttgcatcaaa acttccttca tcagatcttg ttgaagcagg tatcgctgct ccagctcttg
     2581 atttgacaac tgttcttgct gttgcaaaag gctcaaacct taaagttgct gctcaaaact
     2641 gctactttga aaatgcaggt gctttcactg gtgaaactag cccacaagtt ttgaaagaaa
     2701 tcggtactga ctacgttgtt atcggtcact cagaacgccg tgactacttc catgaaactg
     2761 acgaagatat caacaaaaaa gcaaaagcaa tctttgcgaa cggtatgctt ccaatcatct
     2821 gttgtggtga atcacttgaa acttacgaag ctggtaaagc tgctgaattc gtaggtgctc
     2881 aagtatctgc tgcattggct ggattgactg ctgaacaagt tgctgcctca gttatcgctt
     2941 atgagccaat ctgggctatc ggtactggta aatcagcttc acaagacgat gcacaaaaaa
     3001 tgtgtaaagt tgttcgtgac gttgtagctg ctgactttgg tcaagaagtc gcagacaaag
     3061 ttcgtgttca atacggtggt tctgttaaac ctgaaaatgt tgcttcatac atggcttgcc
     3121 cagacgttga cggtgccctt gtaggtggtg cgtcacttga agctgaaagc ttcttggctt
     3181 tgcttgactt tgtaaaataa tcagtaagta gcaaaagcta ggtggaacag cattcagatg
     3241 tctgttacat tttttatagg agagcaaaga ttgaaaacaa aaattggatt agcaagtatc
     3301 tgtttactag gcttggcaac tagtcatgtc gctgcaaatg aaactgaagt agcaaaaact
     3361 tcgcaggata caacgacagc ttcaagtagt tcagagcaaa atcagtcttc taataaaacg
     3421 caaacgagcg cagaagtaca gactaatgct gctgcccact gggatgggga ttattatgta
     3481 aaggatgatg gttctaaagc tcaaagtgaa tggatttttg acaactacta taaggcttgg
     3541 ttttatatta attcagatgg tcgttactcg cagaatgaat ggcatggaaa ttactacctg
     3601 aaatcaggtg gatatatggc ccaaaacgag tggatctatg acagtaatta caagagttgg
     3661 ttttatctca agtcagatgg ggcttatgct catcaagaat ggcaattgat tggaaataag
     3721 tggtactact tcaagaagtg gggttacatg gctaaaagcc aatggcaagg aagttatttc
     3781 ttgaatggtc aaggagctat gatgcaaaat gaatggctct atgatccagc ctattctgct
     3841 tatttttatc taaaatccga tggaacttat gctaaccaag agtggcaaaa agtgggcggc
     3901 aaatggtact atttcaagaa gtggggctat atggctcgga atgagtggca aggcaactac
     3961 tatttgactg gaagtggtgc tatggcgact gacgaagtga ttatggatgg tgctcgctat
     4021 atctttgcgg cctctggtga gctcaaagaa aagaaagatt tgaatgtcgg ctgggttcac
     4081 agaaacggta aacgctattt ctttaataat agagaagaac aagtgggaac cgaacatgct
     4141 aagaaaatca ttgatattag tgagcacaat ggtcgtatca atgattggaa aaaggttatt
     4201 gatgagaacg aggtggatgg tgtcattgtt cgtctaggtt atagcggtaa agaagacaag
     4261 gaattggcgc ataacattaa ggagttaaac cgtctgggaa ttccttatgg tgtctatctc
     4321 tatacctatg ctgaaaatga gaccgatgct gagaatgacg ctaaacagac cattgaactt
     4381 ataaagaaat acaatatgaa cctgtcttac cctatctatt atgatgttga gaactgggaa
     4441 tatgtaaata agagcaagag agctccaagt gatacagaca cttgggttaa aatcatcaac
     4501 aagtacatgg acacgatgaa gcaggcgggt tatcaaaatg tgtatgtcta tagctatcgt
     4561 agtttattac agacgcgttt aaaacaccca gatattttaa aacatgtaaa ctgggtagcg
     4621 gcctatacga atgctttaga atgggaaaac ccttattatt caggagaaaa aggttggcaa
     4681 tatacctctt ctgaatacat gaaaggaatc caagggcgcg tagatgtcag cgtttggtat
     4741 taagcgatga tttgaaagag ggatgtgata gtagcaccct ctttttcttt gttttatgat
     4801 agttcatcct cgagtaaatt caagatcttg ctcggaaatg aagcttatat agtagattga
     4861 atatagacaa ataccttgtg attggtaaaa cattttagaa attcatttac ctttcctaat
     4921 cgacttggtt tcatcttatt tcaatctatt atagtattgg ggaatttctt caaaccacat
     4981 cagcttggtc agttctacct gcgacctcaa aacttgtgct ttggtcaagc tgggtttagt
     5041 ttcctagttt gctgatggat ttccattgac tataagcatc caaccctctt tttgtcttct
     5101 aaagaattct taaattatca gtctattgca acttttctca tataagttct ttgtcttgct
     5161 atttgttttc cttagtagta tactaaggta gtaatcatta agaagtggtt acaaaaaata
     5221 atgaatgagg taaagaaaat ggtagaattg aaaaaagaag cagtaaaaga cgtaacatca
     5281 ttgacaaaag cagcgccagt agcattggca aaaacaaagg aagtcttgaa ccaagctgtt
     5341 gctgatttgt atgtagctca cgttgctttg caccaagtgc actggtatat gcatggtcgt
     5401 ggtttccttg tatggcatcc aaaaatggat gagtacatgg aagctcttga cggtcaattg
     5461 gatgaaatca gcgaacgctt gattacactc ggtggaagcc cattctctac attgacagag
     5521 ttccttcaaa atagtgaaat cgaagaagaa gctggtgaat accgtaatgt tgaagaaagc
     5581 ttggaacgtg ttcttgttat ctaccgttac ttgtcagaac ttttccaaaa aggtttggat
     5641 gtcactgatg aagaaggtga cgatgtgaca aacggtatct ttgcaggcgc taaaactgaa
     5701 acagataaaa caatttggat gcttgcagcc gaacttggac aagcacctgg tttgtaagaa
     5761 ataaggaaac atagataaat ctgtaggaaa tttcttacag attttttcta ttttgtaagc
     5821 tattccaaac cagtcttttt ttgagttttt tgataaaata gtactatcag tgaaaaggat
     5881 ggaagcatga ctaagaaaat cgtagctatt tgggcccagg atgaagaggg tgtgattggt
     5941 aaggaaaatc gtctgccttg gcatttgcca gcagaattgc agcactttaa agaaacaact
     6001 ctgaatcatg ctatcttgat ggggcgtgtg acctttgatg ggatggggcg tcgcttgctt
     6061 ccaaaacggg aaaccctgat tttgacgcgt aatccggaag aaaagataga tggggttgct
     6121 acttttcagg acgtccagtc tgttcttgac tggtatcagg atcaagaaaa gaatctctac
     6181 attatcggtg ggaagcaaat ttttcaggct tttgaacctt accttgatga agtgattgtc
     6241 actcacattc atgctcgggt ggaaggagat acctatttcc ctgaagagct tgacttgtct
     6301 ctttttgaga cagtttcaag caaattttac gccaaagatg agaagaatcc ttatgatttt
     6361 accatccaat accgcaagag aaaggaagta taatggaacg gagtttattt ggtctattca
     6421 cggcttttct gtgtttcatc tgctttctgg caggagcaca ggcttttcgt aaaaagcgtt
     6481 acgggctttc tatcctcctt tggttaaatg cctttaccaa tctggtcaat agtattcatg
     6541 ctttttacat gaccttattt tagatagaat gacagtatag aatggaagga aatcatgtct
     6601 acaaatagaa aaaatgatat gatggtttat tgctcatttt gtggcaaaaa ccaagaagaa
     6661 gtacaaaaaa taattgctgg caacaatgct tttatttgta atgaatgcgt ggagttagct
     6721 caggaaatca ttcgagaaga attggttgag gaagtcttgg cagacttgtc tgaggtgcca
     6781 aaaccaattg aactcctcca tatcttgaac cactatgtaa ttggtcaaga tcgtgccaag
     6841 cgtgccttgg cagtggcggt ttataaccac tacaaacgca tcaatttcca cgatacacgc
     6901 gaagagtcag aagatgtgga tttgcagaag tcaaacattt tgatgattgg cccaactggt
     6961 tcagggaaaa ctttccttgc ccagaccttg gctaagagct tgaatgtgcc ttttgctatt
     7021 gcggatgcga cagctctgac ggaggctggt tatgtgggtg aggacgtgga aaatatcctc
     7081 ctcaaactct tgcaggttgc tgactttaac atcgaacgtg cagagcgtgg cattatctat
     7141 gtggatgaaa ttgacaagat tgccaagaag agtgagaatg tgtctatcac acgtgatgtt
     7201 tctggtgaag gggtgcaaca agcccttctc aagattatcg agggaactgt tgctagcgtg
     7261 ccgcctcaag gtggacgcaa acatccacaa caagagatga ttcaagtgga tacaaaaaat
     7321 atcctcttca tcgtgggtgg tgcttttgat ggtattgaag aaattgtcaa acaacgtctg
     7381 ggtgaaaaag tcatcggatt tggtcaaaat aataaggcga ttgacgaaaa cagctcatat
     7441 atgcaagaaa tcatcgctga agacattcaa aaatttggta ttatccctga gttgattgga
     7501 cgcttgcctg tttttgcggc tcttgagcaa ttgaccgttg atgacttggt tcgcatcttg
     7561 aaagagccaa gaaatgcctt ggtgaaacaa taccaaactt tgctttctta tgatgatgtt
     7621 gagttggaat ttgacgacga agcccttcaa gagattgcta ataaagcaat cgaacggaag
     7681 acaggggcgc gtggacttcg ctccattatc gaagaaacca tgctagatgt tatgtttgag
     7741 gtgccgagtc aggaaaatgt gaaattggtt cgcatcacta aagaaactgt cgatggaacg
     7801 gataaaccga tcctagaaac agcctagagg tgactatgga acttaataca cacaatgctg
     7861 aaatcttgct cagtgcagct aataagtccc actatccgca ggatgaactg ccagagattg
     7921 ccctagcagg gcgttcaaat gttggtaaat ccagctttat caacactatg ttgaaccgta
     7981 agaatctcgc ccgtacatca ggaaaacctg gtaaaaccca gctcctgaac ttttttaaca
     8041 ttgatgacaa gatgcgcttt gtggatgtgc ctggttatgg ctatgctcgt gtttctaaaa
     8101 aggaacgtga aaagtggggg tgcatgattg aggagtactt aacgactcgg gaaaatctcc
     8161 gtgcggttgt cagtctagtt gaccttcgtc atgacccgtc agcagatgat gtgcagatgt
     8221 acgaatttct caagtattat gagattccag tcatcattgt ggcgaccaag gcggacaaga
     8281 ttcctcgtgg taaatggaac aagcatgaat cagcaatcaa aaagaaatta aactttgacc
     8341 cgagtgacga tttcatcctc ttttcatctg tcagcaaggc agggatggat gaggcttggg
     8401 atgcaatctt agaaaaattg tgaggaaaag aaaatggcaa aaacaattca tacagataag
     8461 gccccaaagg ctatcggacc ctatgttcaa ggaaaaatcg ttggcaacct tttgtttgct
     8521 agcggtcaag ttcccctatc ccctgaaact ggggaaattg taggagagaa tatccaagaa
     8581 cagacagagc aagtcttgaa aaacatcggt gctattttgg cagaagcagg aacagacttt
     8641 gaccatgttg tcaaaacaac ttgtttcttg agcgatatga acgactttgt tccttttaat
     8701 gaggtttacc aaacggcctt caaagaggaa ttcccagctc gttcagctgt ggaggtagct
     8761 cgtcttcctc gtgatgtaaa agtcgaaatt gaagtcatcg cagagattgg ataagctagt
     8821 tgaagtttgg tgttgccaaa cttcttttga tataaggaga aaaagatgac aaagaaacaa
     8881 cttcacttgg tgattgtgac agggatgagt ggcgcaggga aaactgtagc cattcagtcc
     8941 ttcgaggatc taggttattt caccattgat aatatgccgc cagctctctt gcctaagttt
     9001 ttgcagctgg ttgaaattaa ggaagacaat cctaagttgg ccttggtagt ggatatgcgt
     9061 agccgttctt tcttttcaga gattcaagct gttttggatg agttggaaaa tcaagatggt
     9121 ttggatttca aaatcctctt tttggatgcg gctgataagg aattggtcgc tcgttacaag
     9181 gaaaccagac ggagtcaccc actagcagca gacggtcgta ttttagatgg aatcaagttg
     9241 gaacgtgaac tcttggcacc tttgaaaaat atgagccaaa atgtggtgga tacgactgaa
     9301 ctcactccac gtgagctgcg caaaaccctt gcagagcagt tttcagacca agaacaagcc
     9361 cagtctttcc gtatcgaagt catgtctttc ggatttaagt atggaatccc gattgatgcg
     9421 gacttggtct ttgatgtccg tttcttgcca aatccctatt atttaccaga actgagaaac
     9481 caaacgggtg tggatgaacc tgtttatgat tatgtcatga accatcctga gtcagaagac
     9541 ttttatcaac atttattggc cttgattgag ccgattctgc caagttacca aaaggaaggt
     9601 aagtccgttt tgaccattgc catgggatgt acgggtggac aacaccgtag tgtggcattt
     9661 gctaaacgct tggtgcagga cttatccaag aattggtctg ttaatgaagg gcatcgcgac
     9721 aaagaccgca gaaaggaaac ggtaaaccgt tcatgagaaa accaaagata acggtgattg
     9781 gtggagggac tggaattccc gtcattctaa aaagtctgcg ggaaaaagat gtggaaatcg
     9841 cagctatcgt gacggtggca gatgatggtg gttcttcagg tgaactccga aaaaatatgc
     9901 aacagttgac accgccaggt gattttcgta atgtccttgt ggccatgtcg gatatgccta
     9961 agttttatga gaaggtcttt cagtatcggt tctctgagga tgccggagcc tttgctggcc
    10021 atccattggg aaatctcatc attgctggct tgtcagaaat gcagggttca acctataatg
    10081 ccatgcagtt attgagcaaa tttttccata caacagggaa aatttatcct tccagtgacc
    10141 atcctttgac ccttcatgca gtctttcagg atgggacaga agtggctgga gagagtcata
    10201 ttgtagacca tcgaggcata attgacaatg tctatgtgac caatgcccta aacgatgata
    10261 cgcctctggc cagccgtcga gtagtgcaga ccatccttga aagtgacatg attgtcctag
    10321 ggccaggttc tctctttacc tctattttgc ccaatatcgt gattaaggaa attgggcggg
    10381 ctcttttgga aaccaaggca gaaattgcct atgtctgcaa tatcatgacc caacgtgggg
    10441 agacggaaca ctttacagat agcgaccacg tggaagtctt gcatcgtcac cttggtcgcc
    10501 cttttatcga cactgtcttg gtgaatattg aaaaagtgcc tcaggaatac atgaattcca
    10561 accgttttga tgaatacttg gtgcaagtgg aacacgattt tgtaggtctt tgtaagcaag
    10621 tttcgcgcgt gatttcatct aacttccttc gtctggaaaa tggcggtgcc ttccacgatg
    10681 gagatttgat tgtggacgag ttgatgcgca ttatacaggt gaaaaaatga gtttcacagt
    10741 agcagtaaaa gaagaaatcc tgggccaaca ccatctgagc tggcatgaat tatctgccat
    10801 tatcaaaatg tctggtagca tcggtctctc gacttcgggc ttgactttgt cagttgtgac
    10861 agaaaatgcc aaactggccc gtcacctcta tgagtccttt ctccatttct atgaaatcaa
    10921 atctgaaatt cgtcaccacc aacggagcaa tcttcgcaag aatcgcgtct ataccgtttt
    10981 tacagatgaa aaggtgcagg atttgttaag tgatttgcac ttggcagact ctttctttgg
    11041 cctggaaaca ggtattgatg aggcgatttt atcggatgag gaagcaggtc gtgcctatct
    11101 ctgtggcgct ttcttggcaa atgggagcat tcgtgaccct gagtcaggca agtaccagtt
    11161 ggaaatcagt tctgtttatc tggaccacgc gcaaggaatt gcctcccttc tccaacagtt
    11221 tttactggat gccaaggtgc ttgagcgcaa gaagggggct gtgacctatc tccagcgagc
    11281 agaagacatt atggacttct tgatagtaat cggagccatg caggcacgtg atgattttga
    11341 gcgggttaag attttgcgag aaacccgtaa cgacctcaat cgggctaata atgccgagac
    11401 agctaatatc gctcggacag tttctgccag catgaagacc attaacaata tcagtaaaat
    11461 aaaagatatc atgggcttag aaaatttgcc agtagatttg caggaagtgg cgcaactgag
    11521 gattcagcac ccagactact ctatccaaca gttggcagat agcctcagca cccctctgac
    11581 caaaagtggt gtcaatcaca gactcagaaa aatcaataag atagccgatg aattataaaa
    11641 ccacgaatcc tatgtgactc gtggttcttt tttataaact agtagagtgt tttggttgta
    11701 ctttttgttc agggtcaatg tagttgatag cgttgttgac agcagttgga gcttctccga
    11761 ggcctgtcgc aatcagatca atttttccgt catagtagca gcagtcaccg atagcataga
    11821 tacctgcttg gctggattcc tgtttgctgt tgacgataat cttgtgacgg ttgaggtcga
    11881 gcccccagtt tttaaggtta ccgacagaag atttgaaacc atagttgaca aagaggtggt
    11941 ctaggtcaat ggtttcagtt tcatcagatt tgacttttgt gatttcaagt ttatcaagtg
    12001 tttttccatt tccaaggagt tggctagggg cgaatggtgt cttgatggtt acagatgatt
    12061 cttgcaaggc ttgaacactg tgttccaagg cacggaaatt atctctgcgg tgaacaaggg
    12121 tagttggtgc gattttttca aaagccaaag cccaatccac agccgagtct cccccaccaa
    12181 gaatcgtcac tttcttacca gcgtattgct gaatgttaga aacgtggtag tggatatttt
    12241 catagccctc aacactttca agttccagcg gacgtggttt gaaggcaccg ccacccatag
    12301 cgatgataac tgttttagtc aggtgacttc ctttagaagt tgtgatggca aattcttctt
    12361 cttgtttgtc aatctcaaga accgtttcat tgagatgaat aggggtatca aatccattta
    12421 gctgttcaat caagcggtta gtcaactctt ctccagtcag gtttgggaag cctggtacgt
    12481 ctaggatttc cttttcaggg tagagaatag caggttgtcc acctagctgg ggaagagagt
    12541 cgatgatttg aaccttggct tggcgtaggt gggcataaaa ggctgcaaaa agcccgacag
    12601 gaccaccacc cacaatggta atatcataga gttgagacat agtttctcct ttgttttttc
    12661 tagtcagttt attttatcat atttttgttc aatgtaaaat ggactaggat agggaaaaaa
    12721 atgccagaaa atggtataat aggacgaaag tgttttgata gggaggatat cagggatgaa
    12781 ttttcaacaa ttatccaatc tgcaatattg gaccagtttg tttgcaagtc catggacgat
    12841 agctatcaat ctgattgata ttttgattgt tgcttatatt ttataccatt ttacaaaagc
    12901 tattgcagga accaagatta tgattttggt acgtggagtt ttggtgttta ttttagctca
    12961 aatccttgca aatatgattg gtttgactac gatttcttgg ttaatcaatc aaattattac
    13021 ttatggggtt attgcggcgg ttgttatctt ctctccagag attcggactg gtttggaacg
    13081 tttgggaaga gcgacagatt tcttttccaa tgcccctatt agtgctgagg aacagatgat
    13141 tcgtgccttt gttaagtctg ttgaatacat gagtcctcgt aaaatcgggg ccttggttgc
    13201 tattcagcgt gtacgtacct tgcaggagta tatgtcgaca ggaattccct tggatgctaa
    13261 gatttctgca gaacttctca ttaacatttt tattcccaac actcccctac atgacggtgc
    13321 ggtgattatc aaagaagaac gtatcgctgt gacgtctgcc tatctgccct tgacaaaaaa
    13381 cacagggatt tccaaggaat ttgggaccag acaccgggcg gctatcggtt tatcagaagt
    13441 ctcagatgcc ttgacttttg tcgtatcaga ggaaacggga ggaatttcga taacctataa
    13501 tggaaggttt aagcacaacc taacacttga tgaatttgaa acagaattac gtgaaatctt
    13561 acttccaaaa gaggaagtgg gtcttagttt taaagaacga ttgctaggag gatggaaaca
    13621 tgaaaaaaaa tagtttatat atcatatcct cactcttttt tgcttgtgtc ttatttgtct
    13681 atgctacggc gacgaatttt caaaacagta ccagtgctag gcaggtaaaa acggaaacct
    13741 atactaatac agtaacaaat gtccctattg acatacgcta taatagtgat aagtatttta
    13801 ttagcggttt tgcttcagaa gtatcagtgg tcttgactgg tgcaaatcgc ctatcgctag
    13861 ctagtgaaat gcaagaaagt acacgtaaat tcaaggttac tgctgaccta acagatgccg
    13921 gtgttggaac gattgaagtt cctttgagca ttgaagattt acccaatggg ctgaccgctg
    13981 tggcgactcc gcaaaaaatt acagtcaaga ttggtaagaa ggctcaaaag gataaggtaa
    14041 agattgtacc agagattgac cctagtcaaa ttgatagtcg ggtacaaatt gaaaatgtca
    14101 tggtgtcaga taaagaagtg tcgattacga gtgaccaaga gacattggat agaattgata
    14161 agattatcgc tgttttgcca actagcgaac gtataacagg taattacagt ggttcagtac
    14221 ctttgcaggc aatcgaccgc aatggtgttg tcttaccggc agttatcact ccgtttgata
    14281 caataatgaa ggtgactaca aaaccagtag caccaagttc aagcacatca aattcaagta
    14341 caagcagttc atcggagaca tcttcgtcaa cgaaagcaac tagttcaaaa acgaattaaa
    14401 aatagaaaag gattttataa caatgggtaa atattttggg actgatggag tccgtggaga
    14461 agctaaccta gaactaacac cagaattagc ctttaaacta ggacgttttg gaggctatgt
    14521 tcttagtcaa catgaaacgg aagcgccgaa agtctttgta ggacgtgaca cacgtatttc
    14581 aggggaaatg ctggaatcgg ccttggtggc aggtctcctt tcagtaggga ttcacgtata
    14641 caaacttggt gtccttgcaa caccagcagt agcttacttg gttgaaactg aaggagcaag
    14701 tgccggtgtc atgatttctg ctagccacaa cccagccctt gataacggaa tcaagttctt
    14761 tggcggtgat ggcttcaaac tagatgatga aaaagaagca gaaattgaag ccttgctaga
    14821 tgctgaggaa gacactcttc ctcgtccaag tgcagaaggc ttaggaattt tggtagatta
    14881 tccagaaggc ttgcgtaagt atgaaggata ccttgtttca actggaactc ctcttgatgg
    14941 aatgaaggtt gccttggata cagctaatgg agcagcttct accagtgccc gtcaaatctt
    15001 tgcagacctt ggtgcccaat tgacggttat cggggaaaca ccagacggtc ttaacatcaa
    15061 ccttaatgtt ggttcaacac atccagaagc ccttcaagaa gtggtcaaag aaagtgggtc
    15121 agctattggt ttggcctttg atggagacag tgaccgcttg attgctgttg atgagaatgg
    15181 taacatcgtc gatggtgaca agattatgta catcatcgga aaataccttt ctgaaaaagg
    15241 acaattggct caaaatacaa ttgtgacaac tgttatgtct aaccttggtt tccacaaggc
    15301 cttgaatcgc gaaggtatta acaaggcagt tactgcagtt ggtgaccgct acgttgttga
    15361 agaaatgaga aaatcaggct acaaccttgg tggtgaacag tctggtcacg ttatcttgat
    15421 ggattacaat accacaggtg atggtcaatt atcagcagtt caattgacta aaatcatgaa
    15481 ggaaactggt aagagcttat cagagttggc ggcagaagta acgatttatc cacaaaaatt
    15541 agttaatatc cgagtggaaa acgtcatgaa ggaaaaggcc atggaagtgc cagctatcaa
    15601 ggccatcatc gagaagatgg aagaagaaat ggcggggaac ggccgtatcc ttgttcgtcc
    15661 aagtggaaca gaacccctct tgcgtgttat ggcagaagcg cctacaacag aagaagtaga
    15721 ctactatgtt gataccatca cagatgtagt tcgtgctgaa attgggattg actaaatcct
    15781 agaaaactag atgaaaataa aaaattatgg tacaatagct tataatattg tagccgatgg
    15841 agaaagacat gaatcttaaa cgtgaacaag aatttgttag tcagtatcat tttgatgcgc
    15901 gtaattttga atgggaaaat gaaaatggag ctcctgaaac caaggtggat gtgaatttcc
    15961 aattgctcca acatgaccaa gaaaatcaag tgacttcctt gattgtcatc ttgagcttta
    16021 tgattgtatt tgataagttt gtcattagtg gaacaatctc acaagtaaac catatcgatg
    16081 gccgtattgt caatgaacca agtgaattga accaagaaga ggtggaaacc ttggctcgtc
    16141 catgtttgaa catgctcaat cgtttgactt acgaagtgac tgaaattgca ttagacttac
    16201 caggaattaa tttggagttc taatatgaaa ttagctgttt tcacagattc ctctgcttat
    16261 ctcagtgaag agaccttgca aagagaagac ttgtttgtct tggatattcc tgtcaatatt
    16321 gatggtgagg aatatgtcga aggcatcaat ctgtctgctg aggaatttta ccaaaaaatg
    16381 gctcaggctt ctgaattgcc taagaccagt caaccaagta ttgccaagtt agatgagatc
    16441 ttaacttcgc tcaaagaaca aggctataca catgccttgg ggcttttcct atcttctgga
    16501 atttcaggtt tttaccaaaa tatccagtat atggtcgatg actatgaggg cttaaccatt
    16561 gctttcccag acactttgat tacaagtgct cccctaggca tcatggttga aagcgtcttt
    16621 aattggcgtg atcagggcga tgattttgcc agcattcagg ataagctagc cattcaaatc
    16681 agccgtacgt cagcctttat catggtagat gacttggatc atttggtaaa aggtggacgc
    16741 ctttcaaatg gggctgccat tttgggcaat ttgcttagca ttaagccaat cctttatttt
    16801 aacgatcaag gtgtgattga agtttacgaa aaagttcgta ctgaaaagaa ggccaccaag
    16861 cgcttaattg aaattatcaa ggaaacaacg gcttcaggtc aataccgggt cattgtcatt
    16921 catgggaatg ctcctgaaaa ggctgaagaa ttgcgtcagc acttgcttga ttttggcttg
    16981 ggttcggatg tttcacttgc tacatttggt agtgtcattg gaacgcacct aggagcagga
    17041 agtattgctc tgggttatat tccagtgatt tagttggcgc ttctaggcta gttaagatgc
    17101 cttgtatctt agaaattgaa cacggactac ctgcctcttg aaaaaagatg cctgtcttgc
    17161 ctttcgtgga aagtcagtgc cattccctat ttttcagagg cagctaaggt cctttgtatc
    17221 ttgataattg aacacgcctg gaaccctgtg tgaaaaagat agttcttcca agaagtagac
    17281 acttcttgat tggaattcct attttcactt tgtgttctta cggactttgt atcttagaca
    17341 ggagtagaga tgagtattcg agtaattatt gccggtttta agggaaagat gggccaggct
    17401 gcttgtcaga tggtattgac tgatccagac ttggacttgg tagcagtttt ggatcctttt
    17461 gagtctgagt cagaatggca gggtattcct gttttcaagg ataaggctga tttagctggt
    17521 tttgaagcgg atgtctgggt agattttact actccagctg ttgcctacga aaatacacgt
    17581 tttgctcttg aaaatggctt tgctccagta gttggaacga ctggtttcac gagtgaagaa
    17641 attgcagagc taaaagaatt ttctcgtgcc caagacttgg gtggcctgat tgcccctaac
    17701 tttgccttgg gtgctgtctt actcatgcaa tttgcgacgc aggctgccaa atatttccca
    17761 aatgtggaga ttattgagct ccatcatgac aagaaaaagg atgctccgag tggaacagcc
    17821 attaaaacag ctgagttgat ggcagaggtt cgagagtcca ttcagcaagg cgcagcagat
    17881 gaggaagagc tgattgctgg tgctcgtggt gctgactttg atggtatgca catccactca
    17941 gttcgtttgc caggtttggt agcccatcag gaagtcatct ttggcaatca gggagaaggg
    18001 ttgaccctcc gtcatgactc ctatgatcgc atctccttca tgacaggagt caatttggga
    18061 attaaagaag ttgtcaagcg tcatgagctt gtctatggat tagaacactt attatgagat
    18121 taacgcaaat gccttctgaa tttcagaagg ctttaccagt attagaaaaa attaaagaag
    18181 caggctttga ggcttatttt gttgggggct ctgttcgaga tgcccttctc cataacccta
    18241 tccacgatgt ggatattgcg acgtcttctt atccagaaga gaccaagcag atttttccgc
    18301 gaacagccga tatcggaatc gagcatggaa ccgtcttggt cttagatggg gatgaggagt
    18361 atgaggtaac aacctttcgg acagaggatg tctatgtgga ctatcgcaga cccagtgcgg
    18421 tttcctttgt gcgctcgcta gaagaagacc tcaaacgccg tgatttcaca gtcaacgcct
    18481 ttgccttgga tgagacagga gaaatcgttg acttgttcca tggtttagaa gatttggaaa
    18541 agcaagtctt gcgagcagtt ggagtggcta gtgagcgttt caacgaagat gctttacgga
    18601 ttatgcgtgg tttccgtttt caggctagtc ttggttttgc acttgaacca gaaacattta
    18661 aagctatgaa gaccttgacg ccgcttttgg agaacatttc tgtagagcgt accttcgtcg
    18721 agtttgataa actcttgctg gctccatttt ggagaagggg cttggcttcc atgattgaga
    18781 gtcaagctta tgactatctc cctgatatgg catctagcca ggacaagctc aacagactgt
    18841 ttgatttgga gactgatttt acttttgaat cctctgaaca agcctgggcg gctttactat
    18901 gggctttgga gattgaaaat gcgcagtcat ttttgaaatc ttggaagacc tcacgccagt
    18961 ttgccaagca agttcaggat ttgctgatta ttttggctct gcgtgaaaat ggagaattga
    19021 gcaagcgaga ttgttatcgc tttgacatag atttgctttt acaggctgaa aatcttcgtc
    19081 aagctcaagg aaaagaagtc aacccacaag ccatcacaga aaaataccaa agcttgacta
    19141 tccatgacaa gaaagagatt cagattaatg gcggtatttt gatcaaggaa tatggctatc
    19201 agccaggccc agacttggga gagattttaa cagagattga gtttgccatt gtcgatggag
    19261 aattggagaa taatcgtgaa gccatccatg cttacctgag ggagaaaaaa tgagtgattt
    19321 tatcgttgaa aaactaagta aatccgttgg tgacaagacc gtttttaggg atatttcctt
    19381 tattatccat gacttagaca gaattggttt aatcggtgtc aatgggactg gcaagaccac
    19441 ccttttggac gtcctttctg gtgtttctgg atttgatggg gatgtcagtc ctttttcagc
    19501 taaaaatgat taccagattg gttacttgac tcaggatcct gattttgatg atagaaagac
    19561 agttttggat acggttctat ctagtgaact caaggaaatc cagctcattc gtgagtatga
    19621 attgattatg ctcgactata gtgaggacaa gcaggcgcgt ttggaacgtg tcatggcaga
    19681 gatggactct ctccaagctt gggaaatcga aagtcaggtc aagaccgttc ttagcaaatt
    19741 gggcattcaa gacttatcta ctcctgttgg ggaattgtca ggtggtctga gaagacgggt
    19801 acagttggca caagtcttac ttggcaacca cgacctcttg cttttggatg agccgaccaa
    19861 ccatctggat attgcgatta ttgagtggct gaccctcttt ttgaaaaatt ctaagaagac
    19921 cgtccttttt atcactcacg atcgttattt cttagacgct ttgtcaacac ggattttcga
    19981 gttggatcgt gcaggcttga ccgagtacca gggaaattac caggactatg ttcgcctaaa
    20041 ggcggaacag gatgagcgcg acgcggctct tcttcacaaa aaagaacaac tctacaaaca
    20101 agaattggcc tggatgcgca gacaaccgca ggcgcgtgcg accaagcagc aggcccgtat
    20161 caatcgattc catgatctga aaaaggaagt ttcaggcagt agtgctgaga cagacttgac
    20221 catgaacttt gaaaccagtc ggattgggaa gaaagtcatc gagtttcagg ctgtttcctt
    20281 tgcctatgaa aacaagccta ttttgcaaga ttttaatctc ttggtgcagg ccaaagaccg
    20341 tatcggaatc gttggggaca atggtgttgg aaaatcaacc ctgctcaact tgattgcagg
    20401 aagtcttgag ccaactaaag gacaagttgt gattggggaa actgttcgca tcgcctattt
    20461 ctctcagcaa attgagggtt tggatgaaag caagagagtt atcaattacc tgcaggaagt
    20521 ggcagaagag gtcaagacca gtggcggttc tacgacttcg attgcagagt tgctggagca
    20581 gttccttttc ccacgttcga cgcatggaac cttgattgag aaattgtcag ggggtgagaa
    20641 aaaacgtctt tatctcctca aactgctttt ggaaaaacca aatgttcttc ttttagacga
    20701 gccaaccaat gacctagata ttgcaacttt gacagtctta gagaatttct tgcaaggttt
    20761 tgcaggtccc gttttaacag tcagtcacga ccgctatttc ttggataagg tagcgaccaa
    20821 gattctcgct tttgaggatg gcaagattcg tcctttcttt ggtcattaca ccgactatct
    20881 tgatgaaaaa gcttttgaaa cagatatggc caatcaagtg caaaaggtcg aaaaggaaaa
    20941 agtggtcaag gttcgagaag acaagaaacg catgacctac caagaaaagc aggagtgggc
    21001 aagtattgaa ggtgatattg aaaccttgga aaaacgtatc gctgctattg aagaggaaat
    21061 gcaggctaac ggctctgact ttggtaagct ggctactctc caaaaagaat tggatgagaa
    21121 aaatgaagca ctccttgaaa aatacgaacg ctatgagtat ctcagtgaat ttgatagtta
    21181 agactattcg aaaatcaaag tgcaaactat gtcggcttca cattgccgac tcaagtacag
    21241 cctgtgaccg gcttcctaat ttgcactttg atttttattg agtataaacg aatagaaaaa
    21301 tcccgtctca tgaaaggatt tctctattct tttttggttt cttgatgaaa gatattttgc
    21361 caggtttcgt ggcgacgcca gatactggta tggatgatac catctaactc atagcagata
    21421 agtttggttt tttgactgat ggaagtaatt tgaatgtcct tgatagcaga atttaactct
    21481 tttttggtct tataagcctc tttatccatc tgctctccat cttggcgaat atagacaaaa
    21541 tcatcagtca agagttcttc tagttggttt ccttggtcga tcaattgttc acgcataagc
    21601 aattttttat agacattgtc taaaatttca tcctcaggga taggtgctgg ttcgatatgg
    21661 acatcggtat caaagacgcc aaaacgctcc tccagcatag actcgacctg atccgcgatt
    21721 tcatggcttt caaaaacaga caagtcagga ttcatctcta gtgtaatatc caggtagatg
    21781 ttgctaccgt aggtgcgacc tctttgcgat ttgaccttgc tgattttggg aatttccatg
    21841 atagcctttt ggtagtcctc gagcaggcgg tcgtcaaagc catctgaaag actaaaggaa
    21901 gactcgatga agatatcata ggcagtcttc aagataaaga aagtgatgat gatagcaacc
    21961 agtttatcca caatcggata attgaaacta ctagctagga tggcaatggc ggttccaagt
    22021 gaggtaacag cgtcagaaag attgtcctta gcagctgcct tcagcgcctt ggagttggat
    22081 ttcttactga ggcgagtatt gtagagatag accacaaaca taatcgctgc agaaatgatt
    22141 cctagagttg caccaagagg atcaatgacc gtttcttccc gactgagaat cttttgaatg
    22201 gtatctctta gaacatcgaa accgacatag aacatgatga tagaagtgat caagcttgcc
    22261 aaatcttcaa tcttccaatg accaaaacgg tggtcacggt ctgcaggctg gcgcgccatc
    22321 cgaatcccga ttaagagggc cacatttcca atgatgtccg atacgttatt aaaaccatcg
    22381 gccaccaaac tggatgaatg aaggagatga ccagttgcta atttggctgc agacaagatc
    22441 aaataggtcg aaatactgat aatggctcca cgctcagcta acttgagatt tgagatagat
    22501 tgcttcattc aacttccttt ctagtcacat agcattctac cattatactg gttttcaagg
    22561 aaaaattcaa ataaggcagt ttttactctt tgaaaactcc ccaaaatttg gtataatggt
    22621 actactcaag ggagtagctg gcagaaacct gtgatagtgt cgtcattccg aattttatgc
    22681 tgaaaagtat gctttccggc cctatcttaa acagcgagac ttgttatgat taacaggtct
    22741 ctttttgttt gtcgtgaaaa gatatgacag aaagagagtc tgttttgcac acaatgtcaa
    22801 ggaggagaca catgtcaaaa gaacaaaaac gccaagcgtt ttatactcag agccctgaag
    22861 aggtcttgca ggctgtggat gcgaccgagc aaggtttgtc atcaagtgag gcggaaaagc
    22921 gccttgccga atttgggcac aatgaactcg aagaaggcga gaaacgatca atcttggtca
    22981 aattcatcga gcaatttaag gatttgatga ttatcatcct agttgcggca gcaatcttgt
    23041 cagtcgtgac ttctggtggg gaagatatcg cagatgccat tatcatccta gctgtggtta
    23101 tcatcaacgc tgcctttggt gtttaccaag aaggaaaagc tgaagaagct atcgaagccc
    23161 tcaaatccat gtctagtcca gttgcccgcg ttcttcgtga tggacacatg gcagagattg
    23221 actctaaaga attggtacca ggcgatatcg ttgcccttga agcaggtgac gtggtaccag
    23281 cggacctacg tttgctagaa gccaactctc ttaaaattga agaagcagcc ttgacaggtg
    23341 aatctgtacc agtcgaaaaa gacttgtcag tcgatcttgc gacagatgct ggtattggtg
    23401 accgtgtcaa catggccttc caaaactcaa acgtaaccta tggtcgtggg atgggtgttg
    23461 ttgtcaatac aggtatgtat actgaagttg gtcatattgc tggtatgctt caagatgcgg
    23521 atgagactga tacaccactc aaacaaaact tgaacaacct ttctaaggtc ttgacctatg
    23581 ctatcttggt cattgccctt gttacttttg tagtgggtgt cttcattcaa gggaaaaatc
    23641 cacttggtga gttgttgact tctgttgccc ttgccgttgc agccattcca gaaggacttc
    23701 ctgctatcgt taccatcgtt ctttctcttg gtactcaagt tttggccaaa cgacattcca
    23761 tcgttcgtaa gttgccagca gttgaaacac ttggttcaac tgaaatcatc gcttctgata
    23821 agactggtac gctgactatg aacaagatga cagtcgaaaa agtcttctac gatgcggttc
    23881 tacatgactc agctgatgat attgaactag gtcttgaaat gccactactt cgttcagttg
    23941 tcttggccaa tgatacgaaa atcgatgtgg aaggtaactt gattggtgac ccaaccgaaa
    24001 cagcctttat ccaatatgcc ttggacaagg gctatgatgt caaaggtttc ttagagaaat
    24061 atcctcgtgt agctgaattg ccatttgact ctgaccgtaa gctcatgtca acagttcacc
    24121 cattgccaga tggtcgtttc cttgtagcag tcaagggtgc gccagaccaa ctcttaaaac
    24181 gttgtcttct tcgtgataag gctggggata ttgctccgat tgatgagaag gttacaaatc
    24241 tcattcatac aaacaattct gaaatggctc atcaagcctt gcgtgtcctt gcaggtgctt
    24301 ataagattat cgatagtatt ccagaaaatc tcacttctga agagcttgaa aatgatttaa
    24361 tttttactgg tttgattggg atgattgacc ctgaacgtcc tgaagccgct gaggctgttc
    24421 gtgtggctaa ggaagcggga atccgtccaa ttatgattac aggtgaccac caagacacag
    24481 cggaagccat tgccaaacgt ttgggaatca ttgacgcaaa cgatacagaa ggtcacgttt
    24541 taactggtgc tgaactcaat gaactgtcag atgaagaatt tgaaaaagtc gttggtcaat
    24601 actctgttta tgcccgtgtg tctccagaac acaaggttcg tatcgtcaag gcttggcaaa
    24661 aacaaggtaa agtcgttgcc atgacaggtg acggtgtcaa tgacgcgcca gctctgaaaa
    24721 cagccgatat cggtatcggt atgggaatca ctggtacaga ggtttctaag ggggcttctg
    24781 acatgattct tgcagatgat aactttgcga ctattatcgt cgcagtggaa gaaggacgta
    24841 aggtcttctc aaacattcaa aagactattc agtacctact ttctgctaat actgctgaag
    24901 tattaaccat cttcctatca accttgtttg gttgggatgt cttgcagccg gttcatcttt
    24961 tgtggatcaa cttggtaacg gataccttcc cagctatcgc tcttggtgtt gaacccgcgg
    25021 agcctggtgt catgaatcat aaaccacgtg gacgcaaggc aagcttcttc tcaggtggtg
    25081 ttttgagttc tatcatttat caaggtgtac tccaagcagc tcttgttatg agtgtttatg
    25141 gccttgcgat tgcttaccca gttcatgtgg gtgacaatca tgctattcat gcagatgccc
    25201 taacgatggc ctttgcaacc cttggtttga ttcagctctt ccatgcctac aatgtcaagt
    25261 ctgtctacca atccatcttg acagttggcc cattcaagtc taagaccttt aactggtcca
    25321 tcttggtatc ctttatcctt ctcatggcaa caatcgttgt agaaccgctt gaaggaatct
    25381 tccacgtaac caaactagac ttgtcacaat ggggaattgt tatggctgga agcttctcaa
    25441 tgattatcat cgtcgaaatc gttaagttta ttcaacgcaa actcggtttt gacaagaatg
    25501 cgatttaagg gaggtttcgg atgtcagcat atcaattacc gaccgtatgg caggatgaag
    25561 ctagtaatca aggagctttt acggggctaa acagacaaac agcaggtgcc cgtttcgaac
    25621 aaaacttgcc aaaaggagaa caagcttttc agctttattc actgggaaca ccaaatggtg
    25681 tgaaggttac tatcttattg gaagaattac tagaagctgg ttttaaggaa gcggcttacg
    25741 acttgtataa gattgctatc atggatgggg atcaattcgg atcagacttt gtgaagctca
    25801 atccaaattc caagattcca gccttattgg accagtcagg tactgaagat gtaagagtct
    25861 ttgagtctgc tcatattctt ctttaccttg ctgagaaatt tggagccttt ttaccaagta
    25921 atcctgtgga aaaggtagaa gttttgaatt ggctattctg gcaagcaggt gcagcacctt
    25981 ttctaggtgg gggatttgga catttcttca attatgctcc tgaaaaattg gaatatccta
    26041 ttaaccgttt tacgatggaa gtgaaacgcc agttggattt attggataag gaattggctc
    26101 agaaacctta tattgcaggc aatgactata cgattgcaga tattgctatc tggtcttggt
    26161 atggacagtt agttcaagaa aatctttacc aaggttctgc aaaattcttg gatgcctcaa
    26221 gttatcaaaa tctagtaaaa tgggcagaaa aaattgccaa tcgtccagct gttaagcgtg
    26281 gcttggaagt aacttataca gaaattaaat agcaaaaaag tcatcttcgg atggcttttt
    26341 gctacagttg agggaaaggg tttgcagtta tcggcttttg tgctataatt gctataatat
    26401 agtaaaaagc aagaggagtg tgaggaagtg gaaaagaaaa ttgtgaagga taacttattt
    26461 ttatctcaag tgtctcagcc ggcaagtcag gaggatcttt atcttgccag agatttgcag
    26521 gatacactct tagcaaatcg tgatacctgt gttggtctag ctgccaatat gattggggtg
    26581 cagaagcgcg tgattatctt taatcttggc ttggttcccg tggtcatgtt taacccagtg
    26641 cttctgtcct ttgaaggatc ctatgaggca gaagaaggct gtttgtcctt ggtaggtgtg
    26701 agatcaacta agcgttatga aaccataagg cttgcctatc gtgacagcaa gtggcaggaa
    26761 cagaccatta ccttgacagg cttcccagct cagatttgcc agcatgagct ggatcacttg
    26821 gaaggacgaa tcatttagga ggaaagcaaa tgaaacgaat agtctttgaa cttattttta
    26881 tcgcaacgac ctggtatatc tttttaccgc cccttaacct gaccagctgg gaatttctct
    26941 tcttcctctg tgggcatttg ttagttgtgg caatcttatt tggctttggc aaggggataa
    27001 accttgtcaa aacggttcat gtgcgccacg gtaaggcgga agctgcctta aatcttgagg
    27061 gtttcaaaat caatcggtta gggaaaattc tgttagcttc gattggagga attcttctct
    27121 tggcagcttt ggtttccttg gtaacttcca gcatgtttca ggctaaaaat tatgccaatg
    27181 tagtcacggt tacggaaaaa gactttactg aatttcctag gagtgacacc agtaaggttc
    27241 ctatcctaga tagaagtact gctgaaaaaa ttggagaccg ctacttgggt tccctaaccg
    27301 ataaggtgtc gcaatacgta gcggcagata cctataccca attgacaatt gatgggaaac
    27361 cttatcgggt cacaccacta gaatatgcag accctatcaa atggtttaac aatcaagcca
    27421 agggaatcgg tgagtatatc aaggtggaca tggtaactgg aaatgcggat ttggtggact
    27481 tgaagacacc aatcaagtat tcagactcgg agtattttaa ccgtgatgtc aaacgtcacc
    27541 tgcgcttgaa gtacccgacc aaaatcttta aaactccatc ttttgaggtg gacgatgagg
    27601 gcaatccttt ctatgtagca acggtttacc aaaagcaatt tggacttgct gttcctcgtc
    27661 ctgcttcagt cattatcttg gatgctacaa atggagaaac caaggaatac agcttatcag
    27721 atgttccaga atgggtggac aggatctatc cagcagagga aaccattgag caaattaact
    27781 acaacggcaa gtacaaggac ggtttcttga atgccatgat ttccaagaaa aacgtgaccc
    27841 agactaccaa gggctataat tacttgtcta tcggtaatga catctatctc tacacaggtg
    27901 tgacgtcggc taatgcggat gagagtaatc ttggtttcat ccttgaaaat atgcgaacag
    27961 gagaaatcac taagtatagc ttggcttctg cgacagaaga atcagcccgt gaatcagcag
    28021 aaggtgctgt tcaggagaaa tcctacaaag caaccttccc aatcctcatc aacctcaatg
    28081 acaagcctct ctacatcatg ggcttgaagg acaatgctgg cttggtcaaa gagtacgccc
    28141 tggtagacgc agtcgagtac caaaatgtta tcgttgctac tacagtggaa gagatgctca
    28201 gcaagtatgc caataaaaac gaccttgaaa ttgacaatgc aacgacagaa agcatcaatg
    28261 gagtagtagc agacctcaaa tcagctgtta tcaagggaga cactgtctac ttctttaaag
    28321 ttgatggcaa gatatacaag gtcaaggctt cagtatccga tgaccttcct taccttgaaa
    28381 atggtaaaac cttcgaaggt caagtaggaa aagacaatta tctcaagacc tttaaagtac
    28441 aataatactc ttcgaaaatc tctagctcta tctgcaacct caaaacagtg ttttgagcag
    28501 cctgcggcta gtttcctagt ttgctttttg attttcattg agtataaaaa aggtttattt
    28561 aataaatata tgttataata aggtaaatta agcctaatgg aggacaaaga aatgggtttt
    28621 tatttgatgg ttgcttcaat gctactcgga ctgctcgctt tgaagatagg tttttctcag
    28681 ttcaaggaga aaaaagataa attcttatct atcttaacaa gtttagctgg cacagccctt
    28741 gttctcgtgg ctgtctggtt aggatggccc aaataaatag aaaaacctcg gtcaaaccga
    28801 ggtttttcaa atgaatttct tggttgtggc taaaaaatat gctacactat caatatgaaa
    28861 attttaatcc caacagcaaa agaaatgaac acagacttgc caagtatcga ggcaattcct
    28921 ttaaaaccag aaagtcagac cgtgcttgat gccttggctc tctattctgc cagtcaattg
    28981 gagagtttct acaaggtatc agctgagaaa gcggcggaag aatttcaaaa tatccaagct
    29041 ttgaaaaggc aaactgctca acactatcca gccttgaaac tttttgatgg gcttatgtac
    29101 cgcaacatta agagagataa gctgaccgag gcggaacaag attatcttga aaatcatgtc
    29161 ttcattacct cggctttgta cggtgttgtt ccagccttgt cacccatggc tcctcaccgt
    29221 ttggattttt tgatgaaatt aaaagtcgct ggtaagactt tgaagagcca ttggaaggca
    29281 gtctatgatg aagctctgaa gaaggaagaa gtgattttct ctctcttgtc atcagagttt
    29341 gagactgtat tttctaagga aatcagagca aagatggtga ccttcaaatt catggaggat
    29401 agaggcggtc agctgaagat tcactcaact atctccaaga aagcgcgcgg ggcctttcta
    29461 acagctttaa tagaaaatca agtacaaact gtgggggaag cacgtcgctt gaactttgct
    29521 ggatttgttt accgagaaga tttgtcacaa ccacagggat tggtttttgt aaaggaagta
    29581 tagaaatcag atattttgta tttggcaata tgtatcatca gctgttttag tttataaatg
    29641 agaaaaaaag cgaacaagct agtcttctga gtgtcagaaa agtagtcttg ttcgctttta
    29701 ttatgttata ctcttcgaaa atctcttcaa gccacgtcag cgtcgcctta ccgtatatat
    29761 gtcactgact tcgtcagttc tatctacaac ctcaaagcag tgctttgagc agcttgcggc
    29821 tagcttccta gtttgctttt tgatttttat tgagtgttag ttacttgata gcttcgacca
    29881 agtcttgagc ttgtttttca agtgagttta ggactgtttc ttcaagaacc aattttccgt
    29941 ctgcccaggc agagtcatta acacgtgcag cagtgaaatc accaacgcct tgtgtacgga
    30001 taaatggcaa gaggtctttg tagatagcga aaagttgatc gtgccctgca ttggctacag
    30061 atgatactgt gacaaacttg tcttgaaggg cagaaacgcc acgtgtatca gacaagtcaa
    30121 gggcacgaga tagccagtca agcaagtttt tcactgtacc agggatagag aagttgtaga
    30181 ctggagagaa aatccagata gcatccgcaa cgagaactgc ttcacgagcg gcagctacag
    30241 ctggatgtgt tgggacttcc aaatcttggc tgaagagagg gagggctgaa taatcaaggt
    30301 agctaacttc cgctttacta gcaagtgctt tctcagcttc gagcgccatt tggtggttga
    30361 aagaaccttg acgtagtgat ccgacgataa ataatacttt tttagacatg atgtgtctcc
    30421 tttttcttaa aatttcgaga actctctcgt tgttataaaa tatattacaa caaaacaaaa
    30481 gaacttgcaa gaaatttgct cagagtttat ttgaaaattg taaaatttcg gaaactctag
    30541 tcatttgtga ctgaaaaagt aagaactgtt tcactttcaa aatcggtttt gtttgaaggg
    30601 agaaatagaa ttggttgctc aaagcacagc tttgaggttg cagataaagc tgacgtggtt
    30661 tgaagagatt ttcgaagagt attcttatat tcacttgtaa taaagcttaa tagatggtaa
    30721 aatagacgag tgaaaaaaag acaaaacaaa gcaaaaaata atctactgtg gcagtatggt
    30781 ctagggatga cgattttgtt tgtggttatc agtggttcct ttctgtatat ggtttctctt
    30841 agcatgaaac cctatcaaac agctaaaagt gaaggagaaa aattagctca gcagtatgca
    30901 ggattagagc aggtcgatca ggttgattta tacaatggct tggaatctta ttacagcgtt
    30961 cttggtcgta ataaacagca agaagcgctt gctgtcctga ttggtaaaga tgatcataag
    31021 atttacgttt atcagctaaa tcagggtgtt tcacaagaaa aagcagaaac ggtttctaag
    31081 gaaaagggag ctggcgaaat tgacaagatt atctttggtc gttatcaaga taagccaatc
    31141 tgggaagtca agtcaggatc tgatttttat ctagtagatt ttgaaacagg agcattggtc
    31201 aacaaggagg gcctatggaa ttatccaacc gtgttttaga aatggaagaa agtgtgactt
    31261 tggctgctgg agcccgtgcc aaagcactga aggctgaggg tagagatatt ttgtctctaa
    31321 ccttgggtga gccagatttc actactccca aaaatatcca agatgccgcc attgcatcga
    31381 ttcgagatgg gcgtgcttct ttttatacag taacctcagg tctgccagag cttaaggcgg
    31441 cggtcaatag ctactttgag cgcttttacg gctattctgt agcgtcaaat caagtgacag
    31501 tcgctgcggg agccaaatat tctctctata ccttctttat ggctgtggtc aatccaggtg
    31561 atgaagtcat catcccaacc ccatactggg ttagctatgg agatcaggtc aagatggcag
    31621 aaggcgttcc cgtctttgtt tctgctaagg aagacaatca ctttaaggtg accgtagagc
    31681 agttagaagc agctcgcact gacaagacca aggttttggt gctgaattcg ccatctaatc
    31741 ccacaggtat gatttacacc cgtgaggaac tcttggcaat tggaaactgg gctgtagaaa
    31801 atgatattct catcctagca gatgatatct atggccgctt ggtttataat ggtcatgagt
    31861 tcacaccgat ttctagccta tcggaagcga ttcgcaagca aacagtggtc atcaatggtg
    31921 tgtctaaaac ctatgccatg actggttggc ggattggtta tgccgttgga gaagcagaca
    31981 ttatcgctgc catgtccaag attgcaggtc aaacaacttc gaatccgtca gcagtagccc
    32041 aatatgcagc agttgaggct ctatcaggtg agcaagatac tgtagaaagc atgcgtcagg
    32101 cctttgagga acgtcttaat accatctatc ccctccttgc agaagtgcca ggatttgaag
    32161 tggtcaaacc gcaaggggcc ttctatctct tcccaaatgt caaaaaggcc atggagatga
    32221 aaggctacac ggatgtgaca gactttacaa ctgttatctt agaagaagcc gaagtggcct
    32281 tggtaacagg agctggattt ggagcaccag aaaatgtccg cctcagctat gcgacagacc
    32341 tagacacgct taaagaagca gtcgaacgct tgaaagcatt tatgggtagt gagaatgatt
    32401 gatcattttg agattaaggt aaaggattta caaatttcag aaggatttta taggagtttt
    32461 ctcgctcctt tggactataa attggctttt aagactagtt ctctaattag ttttctatcc
    32521 ccgaacagcc ctcatcctgg tggtgatttt tggctgactc agggaacaca agatcctgtt
    32581 cattttgctt ttttagcaga aaataaggaa gaagttcagg cttgttatga ggctggctta
    32641 gaggcaggtg ggcgagacaa tggggctcct ggttatcgaa gtgagcatcc gatttactat
    32701 gctgctttta tgattgacct ggatgggaac aatatagagg tggtttgcca taaagaataa
    32761 aaaagaaaag gaaaaataat gacaaaacgt gtaacgatta ttgacgtaaa agactatgtt
    32821 ggtcaggaag tgacgattgg cgcttgggtt gccaacaaat caggaaaagg gaaaatcgct
    32881 ttcttacaat tgcgtgatgg aacagccttc tttcaaggtg tggcttttaa accaaacttt
    32941 gtcgaaaaat ttggtgaaga agtgggactt gagaagtttg atgttatcaa acgcttgagc
    33001 caagaaacgt ctgtttatgt gacaggtatt gtcaaagagg acgaacgttc taaatttggc
    33061 tatgagttgg acatcacaga catcgaagtg atcggtgaat ctcaagacta cccaatcaca
    33121 ccaaaagaac acggaacaga ctttttgatg gataaccgtc acttgtggct acgctctcgt
    33181 aagcaagtag ctgtgttgca aatccgtaac gctattatct atgcaactta tgagttcttt
    33241 gacaagaacg gttttatgaa gtttgacagc ccaattcttt caggaaatgc ggcagaagat
    33301 tctacagaac tctttgaaac tgactacttc ggaacgccag cctacttgag ccaatcaggt
    33361 caactttacc tagaagcagg ggctatggct cttggtcgtg tctttgactt tggtccagtt
    33421 ttccgtgctg aaaaatcaaa aacacgccgt cacttgactg agttctggat gatggatgct
    33481 gagtactcat acttgacaca tgatgagtcg cttgacttgc aagaagctta tgtgaaagct
    33541 cttctacaag gtgttcttga ccgcgcgcct caagccttgg aaaccttgga acgtgataca
    33601 gaactcttga aacgctacat tgcagagcca ttcaaacgta tcacttacga tcaagccatt
    33661 gacctcttgc aagagcatga aaatgatgaa gatgctgact acgagcatct tgagcatggt
    33721 gatgactttg ggtcaccaca cgaaacttgg atttcaaacc actttggtgt gccaacattt
    33781 gtcatgaact atccagcagc catcaaggcc ttctacatga aaccagttcc tggaaatcca
    33841 gagcgcgtgc tttgtgcaga cttgcttgct ccagaaggct atggagaaat tatcggtggg
    33901 tctatgcgtg aggaagatta cgatgccctt gtcgctaaga tggatgaact tggcatggat
    33961 cgtacagaat atgaattcta ccttgacctt cgtaaatacg gtacagttcc acacggagga
    34021 tttggtatcg gtatcgaacg tatggtaacc ttcgcagcag gaacaaaaca tatccgtgaa
    34081 gctattccat tcccacgtat gttgcaccgt atcaaaccat aagaacagga aaacgcccat
    34141 gtggcgtttt ttcgaattct agtgttttta cttgcgtaaa aaaatttttt ctagtataat
    34201 agtttattgt gagcgaacct cacttacccc ttgcaaagtc ttggggtcat tagaccaaaa
    34261 ggaggaacat atcaatggct aaatacgaaa ttctttatat cattcgtcca aacattgaag
    34321 aagaagctaa aaacgctttg gtagcacgtt ttgactctat tttgactgac aacggtgcaa
    34381 ctgttgttga atcaaaaact tgggaaaaac gtcgtcttgc atacgaaatc caagatttcc
    34441 gtgaaggact ttaccacatc gttaacgttg aagcaaatga cgatgcagct cttaaagagt
    34501 ttgaccgtct ttcaaaaatc aacgctgaca ttcttcgtca catgatcgtc aaaattgacg
    34561 cgtaagaagg taaactatga ttaacaatgt tgtacttgta gggcgtatga cacgtgacgc
    34621 tgagttgcgt tataccccat caaatgtagc agttgcgact tttactcttg cagtaaaccg
    34681 tacatttaag agtcaaaatg gtgaacgtga ggctgatttt atcaatgtcg ttatgtggcg
    34741 ccaacaggct gaaaatcttg ctaactgggc taaaaaaggc tcacttatcg gggtgacagg
    34801 tcgtatccag actcgtagtt acgataacca gcaaggacaa cgtgtctacg tgacagaggt
    34861 cgtggctgag aatttccaaa tgttggaaag ccgtagtgtg cgtgagggcc acacaggtgg
    34921 agcttactct gcaccaactg caaactattc agcacctaca aattcagtac cagacttttc
    34981 acgtaatgaa aatccatttg gagcaacaaa cccattggat atttcagatg atgatttacc
    35041 attctaatgg acaactaaac ttataaagga gaaaaaacat ggctcaacaa cgtcgtggcg
    35101 gattcaaacg ccgtaaaaaa gttgattaca tcgcagcaaa caaaattgaa tatgttgatt
    35161 acaaagatac tgagcttctt agccgtttcg tttcagaacg tgggaaaatc cttcctcgtc
    35221 gtgtaacagg aacttcagct aaaaaccaac gtaaagtaac aacagctatc aaacgcgctc
    35281 gcgtaatggc tttgatgcct ttcgtaaacg aagattaaag aaaagacccg gttgggtctc
    35341 tttttcatga gcttgaaaac aagaaagcga attaagaccc caacgggtct tttttcacaa
    35401 gctttgaaat gagagagcga gttaggctca gtggctccct ttttacgagt ttgaaaacaa
    35461 gagaacacat taaaagcacg atggagcttt ttcataaata tgtctttttt ctcatccaag
    35521 aaattaaaat aaaaatgata aactttttga aaatagggga tttgcctact ttatgatata
    35581 atggaaagag ttagattgaa agaggtagtg agatggacgc gaaattaaga tacaaggcaa
    35641 agaagatcaa gattgtcttt tttgatattg atgatacatt gcggaattca aagacagggt
    35701 ttattccaac tacaattccc actgttttta aacagttgcg tgaaaaagga attttaacag
    35761 gaatcgcctc tggacgtggc atttttggtg ttgttccaga gattcgtgat ctcaagcctg
    35821 acttttttgt aactttgaat ggagcttata tcgaagataa aaaaggtcag gtcatttatc
    35881 agcatcagat tgaaaagtca gatgttgagg agtatatctc ttgggctaag caagaaggaa
    35941 ttgaatatgg cttggttggg agtcatgatg ccaagttgtc gactcgcacc gatatgatga
    36001 gtgaagctat caatccaatt tatcccgact tagatgtaga tcccgatttc catgaaaaag
    36061 aagatatcta tcagatgtgg acttttgaag ataagggaga tgacttgcac ttgcctgaca
    36121 gtctctcaga caaacttcgc atggttcgtt ggcatcaaca ttcgtctgat attgtgccga
    36181 tttcaggctc caaagcgacg ggggtggaaa aggttgtgga acaccttggc ttgaaaccag
    36241 agaaggtcat ggtttttgga gatggtctca acgacttgga actctttgat tatgctggaa
    36301 tcagcgttgc catgggaatt tctcatgata aaatcaaaga aaaagcagat tatattacaa
    36361 aaacattaga agaagatggc atttttgatg ccttagaagt atttggtatg gtagaaaaag
    36421 aattgcattt tccacaagta gacattgaaa cagtagaagg tcctcttgcg actattaaga
    36481 ccaatcacgg agacttacgt atcaagcttt tccctgaaca tgctcctaaa acagtggcta
    36541 actttgtatc tctttcaaaa gatggctact atgatggtgt cattttccac cgtattatca
    36601 aggactttat gatccaaggt ggagacccaa ctggaactgg tatgggtggc gagtcaatct
    36661 acggcgaatc ttttgaggat gaattctcag aagagcttta caatatccgt ggtgctcttt
    36721 ccatggcaaa tgctggtcca aataccaacg gcagccagtt ctttatcgtg caaaaccaac
    36781 acctacctta ttcgaagaaa gaaattactc gtggtggttg gccagaaccg attgcagaaa
    36841 tctatgccaa tcaaggtggg acacctcacc tagaccgccg tcacacggtt tttggtcagt
    36901 tagctgatga agcatcttac gctgtcttgg atgccattgc tgctgttgag acaggagcta
    36961 tggacaagcc agttgaagat gttgtaattg aaactattga aatcgaggac taagatgaaa
    37021 atcggtgata agctaaaggg gcgtattaca gggattcagc cctacggtgc ctttgttgag
    37081 ttagagacgg gtgatacggg gctgattcac atttcagaaa ttcggacagg atttattgaa
    37141 aatattcatg aaaccttgaa agtcgatgaa gaggttcaag ttcaggtagt ggatttagat
    37201 gaatttacgg ggaaagccag tctttctatc cgcactttgg aggaagaaaa gtaccagttt
    37261 cctagacgga gacgcttttc aagcgaccgc tttaactacg gttttgctcc ctttcgacga
    37321 atgctaccaa tctggactgg agaagccctg caccatttaa aaaagaagaa gtagtttaga
    37381 gttttatatt ttttgtaatg ttaatatgaa tttgctataa taggaccatg gaagaagaac
    37441 aattattaaa atcaggagag cgcattaacc agctcttttc gacagatatc aaaatcattc
    37501 aaaatagaga ggtttttagc tattcggtgg atagtgttct cttatcacga tttccacgtt
    37561 ttcctaagaa ggggttgatt gtggatttct gtgctgggaa tggagcagtg gggctttttg
    37621 ctagcactcg tactcaagca cagatattgt ctgttgagat tcaggagcgt ttggcggata
    37681 tggctgaacg ctctgtccgt ttgaatggat tagaggagca aatgcaggtc atctgcgatg
    37741 atttgaaaaa tatgcctgct cacattcagg ggagtaaggt ggatatgatt ttgtgtaatc
    37801 caccctattt caaggtgaat ccttattcta atctgaacga gagtgaacat tatctcttgg
    37861 ctcgacacga aatcacgact aatttggaag aaatctgtcg tagtgcccag agtattctca
    37921 agtctaatgg gcgtttggcc atggttcatc gtcctgatcg acttctggat attttggata
    37981 cgttaaaacg acataatcta gcccctaagc gcctgcagtt tgtttatcca aaaagagaaa
    38041 aggaagccaa tatgcttttg attgaggcga tcaaggatgg ctcaacaagt ggctttaagg
    38101 tcttacctcc tttgattgtc cacaatgatg atggctctta tacgcccgaa atcgaagaga
    38161 tttattatgg atcataaggc ttatatgtac gttctggagt gtcgtgatgg atcctactat
    38221 ataggctata cgactgatat gagaagacgc ctcgctatcc acaatagtgg gaagggagcc
    38281 aaatatacac gagcacgctt gccagtcaaa cttatctatg ctcaaggttt tgccagtaag
    38341 gaagaagcca tgtcggctga agctcttctc aagcgtaaga agaggccaca gaaggaagaa
    38401 tttttatctg aaaatcaaga tagaaattta ctcagttatt ttgaagaatc gtggggagtc
    38461 ctttgactcc tcttaccttt gtcaaaaagc gaaaaaaatg ctacaataaa gaaaataaac
    38521 aaaatgaggt attatcatgt ccaagattct agtatttggt caccaaaatc cagactcaga
    38581 tgccatcgga tcatctgtag cttttgccta ccttgcaaaa gaagcttacg gtttggatac
    38641 ggaagctgtt gcccttggaa ctccaaatga agaaacagcc tttgtcttga actattttgg
    38701 tgtggaagca ccacgtgtta tcacttctgc caaagcagag ggggcagagc aagttatctt
    38761 gactgaccac aatgaattcc aacaatctgt atcagatatc gctgaagtag aagtttacgg
    38821 tgttgtagac caccaccgtg tggctaactt tgaaactgca agcccacttt acatgcgttt
    38881 ggagccagtt ggatcagcgt cttcaatcgt ttaccgtatg ttcaaagaac atggtgtagc
    38941 tgtgcctaaa gagattgcag gtttgatgct ttcaggtttg atttcagata cccttctttt
    39001 gaaatcacca acaacacacc caacagataa aatcattgct cctgaattgg ctgaattggc
    39061 tggtgtgaac ttggaagaat atggtttggc aatgttgaaa gctggtacca acttggctag
    39121 caaatctgct gaagaattga ttgatatcga tgctaagact tttgaactca acggaaataa
    39181 tgtccgtgtt gcccaagtga acacagttga catcgctgaa gttttggaac gccaagcaga
    39241 aattgaagct gcaatgcaag ctgccaacga atcaaacggc tactctgact ttgtcttgat
    39301 gattacagat atcgtcaact caaactcaga aatcttggct cttggtgcca atatggacaa
    39361 ggtcgaagcg gctttcaact tcaaacttga aaacaatcat gccttccttg ctggtgccgt
    39421 ttcacgtaag aaacaagtgg tacctcaatt gactgaaagc tttaatgcgt aagattttgg
    39481 gtgtcagctc aaaatcggaa agtctagttt gccttatatc gaaaggagtt tcggctcctt
    39541 ttttctagga gtgaagtatg ttagaaaatg gcgatttgat ttttgtgaga gatgggtcag
    39601 acatgggaca ggccatccag acttccacag gtaactatag ccatgttgcc atttatttgg
    39661 atgggatgat ttatcatgct agtggacagg ctggtgttgt ctgtcaagaa ccggcagact
    39721 tctttgagtc caatcattta tacgacctct atgtttaccc agaaatggat atccagtcgg
    39781 tgaaggaaag agcttgcaaa catcttggag caccctacaa tgcttctttc tatccagatg
    39841 cacctggttt ctactgctcc cagtatatag cagaaatcct acctattttt gaaactattc
    39901 ctatgaaatt tggagatggg gagcaggaga ttagtgattt ttggagggag tattacatag
    39961 aactaggtct gcctgttcct ctgaaccaag ctggtaccaa tcctagtcag ttggcagcat
    40021 cgcctctgtt acaatgtaaa gaaaggaatc ttcatgattc agatttttaa tccatctcgt
    40081 ttgacgagac agccattttt ggagaattga tccgctatct ggaccagtat gaggatgtga
    40141 ttctacggga aattaaggct caatttccag atgttgcagt tgataaactc atggaagagt
    40201 atataaaggc aggcttgatt ctacgtgaaa ataagcgcta ttacctcaat tttcctacgc
    40261 ttgaatcact tgatagtctt gaactggatc aagagatttt tgtcagagaa gctagtccgg
    40321 tctatcaagc cttgttggag cagagttttg agacggaatt gcgcaatcaa atcaatgcag
    40381 ctattttagt tgaaaagacg gactttgcgc gcattaaaat gactctgtcc aattattttt
    40441 acaaggtcaa acagcagtat cctttgacag aaaaacagca ggagctctat gacattttag
    40501 gagatgttaa tcctgagtat gcactcaagt atatgacggc ttttttgttg aaatttctca
    40561 aaaaagacca gcttatgcag aaatgccgtg atatctttgt ggacagtttg gttgtcttag
    40621 gctatattgt gcaaaatgaa gatggaaagt atgagttggc tatcgatttt gataaggaga
    40681 gattaacttt ctacttagcg tgatttcttg tttctgagta cattgtttga ctttccttag
    40741 tattcggtat aaactatatg taaccggtaa cacatatcgg aataaactaa aggagacaat
    40801 catatgtcac ttgaaaacaa attggaacaa gcaacaggcg ctgtcaaaga aggttttggt
    40861 aaagttactg gagacagcaa gacagaactt gaaggagctg ttgaaaaaac agttgctaag
    40921 gcaaaagacg ttgtagaaga cgcaaaaggt gctgtagaag gtgccgttga aggtttgaaa
    40981 aacgttttta ctaaagaata ggaaaaaatc aagggtttca ttttcccttg attttttcta
    41041 ttcttataaa taattttctg cgacggctgt atctcctggg taggattctt tcttgccctg
    41101 gatgatttgg taacaatcgg ctcccttacc cgcaataata actgcatcta attcgtgatt
    41161 tgtgatagcc attgccgcct tgatggcttc ttggcgatcc gcaatcttct caacaggatg
    41221 attgatgtag ctactaattt catctgcaat ggccattggg tcttcatagt tagggtcatc
    41281 agcagtcaga aagacttgaa tctcagggtg ttgattgagg aggaggccaa agtccttacg
    41341 acgactttct cccttgtttc ctgttgatcc cagaaccaga gcaatctttc cggtttgatg
    41401 agtttcaacc acattgatga gttttttcag actatcccca ttgtgggcat agtcgatgaa
    41461 gaccttggct ccatttttct gagtgaggac ttccatacga ccaggaacgc gggttgcagc
    41521 gatgcctttt ttgatgtcct caagacttgc tccgagacgg agacaagcaa gtccagcagc
    41581 aactgcattt tcttggttga agttgccaat gagttggata tcataatctc cagcgagttt
    41641 acccgtagct gaaaagctaa aggctttgga attctcgatt tggttatcaa attggctacc
    41701 atagaaatca tggtcttgat cttcaacctg ttctttcaag actgagaagt ggtccatgtc
    41761 actgttaatg atgactgctc ggctattttc catcaagaga cgcttgtggt agaaatagtc
    41821 ttcaaagcta gggtgttcaa tcgggccgat atggtctggg ctgatattga ggaaaactcc
    41881 cacatcaaag gttagaccat agacacgtct gaccagatag gattgactgg agacttccat
    41941 gatgaggtgg gtacggtcat tttgcacagc ctgattcatc atgtcaaaga ggtcaatact
    42001 ctcaggggtt gtcaacgctg acttaaagaa agtctcgcca tcaagagttg tgttcatggt
    42061 cgacaacata gcaggtcgat gcccttgaga taagatgtta taggcgaaat aggctgctgt
    42121 tgtcttaccc ttagtaccag taaaggcaag gagtttgagt ttttcctgtg gattaccata
    42181 gaactccatg gcaatcaaac tcatggcttt ctttatatcg ttcacaatga tgacagggat
    42241 accgacttcg tagtcctttt cagctacata ccaagctaag ccttgtgtta tagcagaaag
    42301 aaggtattct tttttaaagg cagcgccttt tgcaaaaaaa agagtgtctt ctgttacttt
    42361 tcggctatcg tagctgatgc tatcaaaaac aactttgctg tagttgtagt ggtaatgacc
    42421 ttggtcaata atttcgcgaa aaaggccatc tttctttaaa atatctaata cagtttcaat
    42481 ttttatcata ctttctattg taaaccgaaa gtcgcaaatt tacaagtaac aaggaaaagt
    42541 ttataatgga agataaggag tttttcctag ttatcaaaat tgaatgagga atctatgtcg
    42601 cacgaaaaca atcaccagca ggcccagatg ttacggggga ctgcttggct aacggctagt
    42661 aactttatca gtcgcctact cggggctgtt tacattatcc cttggtacat ctggatgggg
    42721 gcttatgcag ctaaggcaaa tggtctcttt accatgggtt acaatatcta tgcttggttc
    42781 ttgttggttt caacagcggg gattccagtt gcggtggcca agcaagttgc caagtataat
    42841 accatgcgag aagaagagga tagctttgcc ctgattcgga gcttcttagg ctttatgaca
    42901 ggactaggcc tggtttttgc tttagtcttg tatgtctttg ctccttggct agcagacttg
    42961 tctggcgtgg gcaaagactt gatcccaatc atgcaaagct tggcttgggg agtcttgatt
    43021 ttcccgtcta tgagtgttat ccgaggattt ttccaaggga tgaataacct caaaccttat
    43081 gccatgagcc aaattgctga gcaggtcatt cgtgttatct ggatgctcct agcaaccttt
    43141 atcattatga agctcggttc aggagattat ctagcagccg ttacccaatc aacctttgct
    43201 gcctttgtcg gtatggtagc cagttttgca gtcttgattt atttccttgc ccaagaaggt
    43261 tcactcaaaa gagtctttga aacaggagat aagattaaca gtaagcgtct cttggttgat
    43321 accattaagg aagccattcc ttttatcctg acagggtctg ccatccagct cttccagatt
    43381 ttggatcagc tgacctttat caatagtatg agctggttta ccaactacag caatgaggac
    43441 ttggttgtca tgttttctta tttctcagcc aatcctaata aaatcacgat gattttgatt
    43501 tctgtagggg tttcgattgg gagtgttggt ttgccacttt tgacggaaaa ctatgtcaag
    43561 ggggacttga aagcagcttc tcgtctggtt caggacagtc tcaccctact ctttatgttc
    43621 ttgctaccag caacggttgg agtggttatg gtaggagaac ctctatatac agtcttctat
    43681 ggtaagccag atagtttggc tctgggctta tttgtctttg cagttttgca gtctattatt
    43741 ttaggcttgt acatggtctt gtctccaatg cttcaggcca tgttccgcaa ccgcaaggcc
    43801 gttctctatt ttatctatgg ttctattgcc aagctagtct tgcaactacc taccatcgcc
    43861 ctcttccaca gttatggtcc tttgatttca acaaccattg ctctcatcat tcctaacgtc
    43921 ttgatgtatc gggatatttg taaagtaact ggtgtcaagc gcaaggtgat tttgaagcga
    43981 accattttaa tcagtttgct gaccctagtc atgtttctgt taataggaac catccagtgg
    44041 ctgttaggat ttttcttcca accaagtgga cgtttgtgga gcttctttta tgtagctctt
    44101 gtcggtgcca tggggggtgg actttatatg gttatgagtc tgcgtaccta tttattagat
    44161 aaggtaatag gaaaagccca agcagatcgc ctgcgagcaa aatttaagct ttcgtaaggt
    44221 agcatttata aataaaagga ataagttgaa caatctttta ggagaacttg aaacttattc
    44281 cttttttagc gtcaaaatag ctaaaattat ctttcaaaaa tagaaaatga ggtttttttg
    44341 attttttgga aaaaacaatt gactaaacaa accaagagtt gtataataaa acaaatattt
    44401 aaagcaggag gttctggaaa tgaaaaagtc taagagcaaa tatctaacct tggcaggtct
    44461 tgtcctgggt acaggagttt tattgagcgc gtgtggaaat tctagcactg catcaaaaac
    44521 ctataactat gtttattcaa gtgatccatc tagtttgaac tatctagcag aaaaccgcgc
    44581 agcaacatcc gatattgttg caaatttggt agacgggtta ttagaaaatg accaatatgg
    44641 gaatattatt ccatcattag cagaggattg gactgtttct caggacggtt tgacctatac
    44701 ctacaaactt cgtaaggatg ccaagtggtt tacttctgag ggagaagaat atgcgcctgt
    44761 aactgcccag gattttgtga caggtttgca atatgcagct gataaaaaat cagaagcttt
    44821 gtatctagtg caggactctg ttgctggttt ggatgactat atcactggta aaacaagcga
    44881 cttttcaact gtcggtgtca aggcacttga tgaccaaacg gttcaatata ctttggttaa
    44941 accagaactt tactggaatt caaaaacact tgcaacgata ctttttcctg ttaatgcaga
    45001 tttcctgaaa tcaaaagggg atgattttgg gaaggcggat ccatctagta ttttgtacaa
    45061 tggacctttc ttgatgaaag cacttgtctc aaaatctgct attgaatata agaaaaaccc
    45121 taattactgg gatgctaaga atgtctttgt agacgatgtg aaattgacct actatgatgg
    45181 tagcgaccaa gaatcactgg aacgtaattt tacagctggt gcttatacta cggctcgtct
    45241 ttttcctaac agctccagct atgaagggat taaagaaaaa tacaaaaaca atatcatcta
    45301 tagtatgcaa aattcaactt catatttctt taattttaac ctagatagga agtcttacaa
    45361 ttatacttct aaaacaagtg acattgaaaa gaaatcgacc caggaagcag ttctcaataa
    45421 aaacttccgt caggctatca attttgcttt tgacagaaca tcttatgggg ctcagtctga
    45481 agggaaagaa ggtgcaacaa agattttgcg taacctagtg gttcctccaa actttgtcag
    45541 tatcaaggga aaagactttg gtgaagttgt agcctctaag atggtcaact atggtaagga
    45601 atggcaaggt atcaactttg cggatggtca agacccttac tacaatcctg agaaagccaa
    45661 ggctaagttt gcggaagcta agaaagaact cgaagcaaag ggtgttcaat tcccaatcca
    45721 cttggataag actgtggaag taacagataa agtaggcata caaggagtta gttctatcaa
    45781 acaatcaatt gaatctgttt taggttctga taatgtagtg attgacattc agcaattaac
    45841 atcagatgag tttgacagtt caggctactt tgctcaaaca gctgctcaga aagactatga
    45901 tttatatcgt ggcggttggg gacctgatta tcaagacccg tcaacctatc tcgatatttt
    45961 taatactaat agtggaggag ttctacaaaa tcttggacta gagcctggag aagccaatga
    46021 caaggctaag gcagttggac tggatgtcta tactcaaatg ttggaagaag ctaataaaga
    46081 gcaagatcca gccaaacgtt atgagaaata tgctgatatt caagcttggt tgattgatag
    46141 ttctttagtt cttccaagtg tttcgcgtgg gggaacacca tcattgagaa gaaccgtacc
    46201 atttgctgct gcctatggtt taaccggtac aaaaggggtt gaatcatata aatacctcaa
    46261 agtacaagat aagattgtca caacagacga atatgcaaaa gccagagaaa aatggttgaa
    46321 agaaaaagaa gaatccaata aaaaagccca agaagaattg gcaaaacatg tcaaataaaa
    46381 tctaattgta gataagtttg tgtaagaagt ctttgtgctt aaaaacgcat aatatcaggt
    46441 atataaaatc ctagatgtta tgcgttttgt tataggaaga tttattagat tttatcctca
    46501 aattgagtta ttcccgactt tatccgttga aatattataa ggagtattta tgaaattaaa
    46561 attattaaga gtagatacta aggtgattat ggggagtttc ttacttgttc tgtctagtct
    46621 acttgctttg ttgcttcccc ttatcttaaa gggtttaata gatgggagtt ctattgaaaa
    46681 tataggttcc aaagtatttc aatcgttttt gatttttatt ggtcaagcct tgttttcttc
    46741 tattggttac tatctgttta gtcaatcggg tgaaaaaaag atagcaaaaa tcaggaaaaa
    46801 agtgatagag gggttgattt atgcagagaa atccttcttt gataagagcc aaagtgggga
    46861 gttgacttct gccattgtca atgacatgag tgtcattcgt gagtttttaa ttacgacttt
    46921 cccaaatatt attctgagtt tagttatggt acttggttcc attgtagtct tatttagtct
    46981 tgattggaat ctttctctac ttttattcat cactcttcct tgtatgatgt ttattatctt
    47041 gcccctttcc aatatcagtg aaaagtatag tcgtcgttta caggaggaaa tttaacaggg
    47101 caattgactg aaaagattca agaacatgaa ttaattaaga ctaaccaagc agagaaaagt
    47161 gtacaggatg ttttggataa ttgtattgaa agggtacaaa acaattcact gaaatcagat
    47221 agggttactt cttttgagac ctcgtttgct ctcttattta tctttgcgac tatagttgtg
    47281 atgctaacct atgggggtta tcgggtcagc gcaggatata tatctgtggg aaccttggtt
    47341 tcgtttttga tttacctctt tcaattactt aatcctatta gtaatatagc taattttgta
    47401 actgtttatt ctaggagcaa gggatcttca gttgcactgg ataacttgct tgcagttcct
    47461 aaagaaaaat ttgagggagg aaaatcggta tcaggacaag ggttgaattt taaccatgtc
    47521 tattttggtt atgatgaaaa tcgacctgtc ttaaaggata ttacttgttc aattttcaag
    47581 gggcaaaaat tgcttttgtt ggaccatctg gatcaggaaa atcaacgatt gtgcgtttgt
    47641 tagagcggtt ttataaaccg ctttcaggag atattctaat ggagcaatca agtatatatg
    47701 attttaactt aaaagaatgg agaagtaaaa tcgcttgggt ttcacaaaat aatgcagtct
    47761 tatctggcag tattcgtgac aatctttgtc tcggtttgaa tcgcttagta actgatgatg
    47821 aattgatgaa agtgctagac ttagtatcac taggtgatga gattcgctcc atgaaagagg
    47881 gactagatac tgaagttggt gaacgcggac gattcttgtc aggggggcaa agccaaagac
    47941 ttcaaatagc tagaacctac ttaaaagatg ctgaaattct tatatttgat gaagctactg
    48001 ctaatcttga tgcggattct gagtatgcga ttatcagtag cctctattct gtattaaagg
    48061 agaagacggt tgtgattata gcgcatagtt tgtcaacggt aaaagatgtg gattgtattt
    48121 tcttcttaga ggaggggaaa atcactggct caggaactca taaggaacta ctggaaaatc
    48181 atgagcgtta tgctcgtttt gtgcaggagc aaatgataga gtgaagtgtc ttttgagatt
    48241 caccatttta tagtctatta aagggagcag gaaaaactcc ctttttatat agtttgaaac
    48301 tataactagc tcttgaaaag aagaaaatga gttgatgaaa ataagtggta caatagttac
    48361 tatagatttg gaggtattgt atgagcaagg aattacacat taacacaatt ttggcccagg
    48421 cgggtattaa gtcagatgaa gcgacaggtg cattggtgac accgcttcat ttttcaacga
    48481 cctatcagca tccagagttt ggtcgatcta ctgggtttga ctatacgcgc actaaaaatc
    48541 caactcgtag taaggctgag gaagtcttgg cggctattga gtcagcagac tatgccttag
    48601 cgactagctc agggatgtca gctattgtac tggcctttag cggctttcca gtaggaagta
    48661 aggtcttggc agtgcgtgat ctttacggcg gttcttttcg ctggtttaac caagtggagc
    48721 aggaaggtcg tttccatttt acctatgcca atacagaaga agagttgatt gccgagttag
    48781 aaaaggatgt ggatgttctc tatatcgaaa ccccaaccaa tcccttgatg ttggaatttg
    48841 atatcgaaaa actagcaaaa ttggctcatg ctaagggtgc caaagtggtg gtggacaata
    48901 ccttctatag ccctatctac caacgtccga ttgaagatgg agcagatatc gttctccatt
    48961 cagcaaccaa gtatctagca ggccacaatg atgtcttggc tggagtggtt gtgaccaata
    49021 gtttagaact atacgagaag cttttttaca atcttaatac aacaggggca gtcttgtctc
    49081 catttgacag ctatcagttg cttcgtggtc tcaagacctt gtctcttcgt atggagcgct
    49141 caacagctaa cgcccaagaa gtggttgcct ttttgaagga ttctccagca gttaaggaag
    49201 ttctctacac tggtcgtgga ggcatgattt cctttaaagt agccgatgaa acacgcattc
    49261 ctcatatttt gaacagtctc aaggtcttct cttttgcgga aagtttgggt ggagtggaaa
    49321 gtcttattac ttatccaacg actcaaactc atgctgatat tccagcagag gtacgccatt
    49381 cttatggttt gacagatgac ctcttgcgtt tgtctattgg gattgaggat gctagagatt
    49441 tgattgcaga tttgcgccaa gccatagaag gataagacaa agatgggaaa atatgatttt
    49501 acaagcctgc ccaaccgttt agggcaccat acctataaat ggaaagaaac agaaacggat
    49561 agtgaagttc taccagcttg gatagcggat atggactttg tggtcttgcc tgaaatccgc
    49621 caagccgtgc aaacttacgc agaccaactg gtttatggtt atacctatgc cagtgaagac
    49681 ttaattaagg aagttcaaaa gtgggaagct acacaatacg gttacaactt tgacaaagag
    49741 gctcttgtct ttatcgaggg tgtggtacca gccatctcaa cagctattca aacctttaca
    49801 aaagaaggcg aggcggtttt aattaacacg cctgtctacc caccctttgc tcgcagtgtc
    49861 aagttgaata atcgtagatt gattactaat tccttagtgg aaaaggatgg tctgtttgag
    49921 attgactttg accaacttga aaaggatttg gtggaagagg aggttaaact ctatattctt
    49981 tgcaaccctc acaatcctgg tggacgtgtt tgggaaaaag aagtgttgga gaagattggc
    50041 caactctgcc aaaaacacgg tgttttgtta gtttcggatg agattcacca agatttgacc
    50101 ctctttggtc acaaacacca gtctttcaat accatcaatc ctgccttcaa aaattttgct
    50161 atcgtcttga gcagtgccac taaaacattt aatattgctg gaacaaaaaa ttcctatgca
    50221 gtcattgaaa atcctaagtt gagactagct ttccagaaac gcctgttggc caataatcag
    50281 catgaaattt caggcttggg ttatttggcg acagaagctg cctatcgata cggtaaagat
    50341 tggctagagg aactcaagca agtctttgaa gaccacatca attatgtggt ggatctattt
    50401 ggaaaagaga ctaaaatcaa ggtcatgaaa ccgcaaggta cctacttgat ttggcttgac
    50461 ttttcagctt atgacctgac tgatgaaaca ttgcaagagt tgttgagaaa tgaagccaag
    50521 gttatcctca accgtggttt ggattttgga gaggaaggaa gtctccatgc ccgcatcaat
    50581 atagctatgc ccaaatctct gttgcaagaa gtctgtcagc ggattgtggc tacttttgcc
    50641 aaacgttaaa aatccagcct tctaggagaa aagtcttcct agaaggctat tttcataggc
    50701 gaaaatatgg tataataaaa agataaggta aaggtgaaaa tatggctaaa ttgattccgg
    50761 ggaaaattcg tatcgaaggt gttgcccttt atgaaactgg taaggttgat atcatcaagg
    50821 aaaagaacaa tcggctctac gctcgcgttg cagaagaaga actgcgctat agtttagagg
    50881 atgatttggt tttttgtgcc tgtgattttt ttcaaaagag gggttactgt gtgcatttgg
    50941 cagcgctaga gcattttctg aaaaatgatg agcgtggtca ggaaatcttg tggagtctgg
    51001 aagaaggtca tgaagaaaaa gaggctgttg aaaccaaggt gaccttgggt ggcaagtttt
    51061 tggatcgaat tttatctcca aaatcagaat gcgcctatga gttatcagct gtgggtcagg
    51121 tggaagcagg aaccaatcat atcttgtgga ctctgagaat cggccaaatc aatagccaaa
    51181 aatactatgt cattcgggat attccacttt ttttaaggat tgtcgagcag agaaagtcgt
    51241 atatgattgg caagatttat gaggagtctc tttcttggga agcctttgat gatgccagcc
    51301 aagaactttt gactttcttg cacggactaa cggaggaagg gctggtttca gacctctttt
    51361 tccaaaacca aggtcggcac ctctttttcc ctctgacctt ttttgagcag ggtgtgaatt
    51421 tactgatggc cttgccccat tttcaatttg accatcaagt ggatagctac caaaccctgc
    51481 tttttcagga tatgcatgct gatgccaatc tatttgcctt tacagtaaca gaatactcgg
    51541 actattttga aatggaaatc agtgagagtc ctagggtcaa tgtcttttat cagggagcag
    51601 tacttttcca taaaggtcag gtttattttt taacagacca gcagatgcgt cttctcaagg
    51661 aaattaaagc gttacctgtg ggtcaacaag gaaagaaata cctgcaattt gatagcagtg
    51721 atcgagacaa attggcctcc tgtctgactc tttttatcca gatgggcacc gtttcagctc
    51781 cagaacgtct acaaatcaaa acttttgcgc cctcttttta ctttgacagg gaagaagata
    51841 atcggattcg tttagaaatt cagtttgatt atggaaatag acaggtatct agccgacaag
    51901 agctagaaga attaccattt tcaagtgacg cggacttaga agaaagaatt ttccaagtct
    51961 gtttggctgc gggctttgaa gccgattttc aatcttggcg tcaggcctta aaagcggagt
    52021 ctgtctatca tttttttcat gatatcattc cagtctttga aaaactcggg catgttgacc
    52081 tatcagataa actagaagag ctttatagcc tagcgagtcc tcaggtgcag attgcctcca
    52141 agggaggtct cttggaaatc cagtttgatt ttcaagatat tgcccaggag gaaatcgacc
    52201 aagccatgca ggccttggtt gccaatcagg atttttatat tgattcgtct aatcaagttt
    52261 actttttcga tgaagaaacc aagaaaattc gccaaaatct acaggaactg gggcaatttg
    52321 aattaaaaga tgggaccttg caggctcgga aatccttggc ctacagttta gctcatctct
    52381 ttgaagggcg agaccgtgtt tctttttcac aagaattcca gaatctggcc caggatttga
    52441 cacatccaga ggactttcct cgacaggcaa ctcaggttca ggctgacttg cgagattatc
    52501 aggaaaaggg aattggctgg ttgcagatgc tccatcatta tggttttggt gggattttgg
    52561 ctgatgatat gggacttggg aaaacccttc agaccattgc ttttttgact agtcaagtga
    52621 caaaagaaag tcgggttttg attctcgctc cgtcaggttt gatttacaac tgggcagatg
    52681 agtttcagaa atttgcccca cagttggatg tggctgttgt tcatggtttg aaagcaagtc
    52741 gtgaggagat tcttgccgag agccatcaaa tctatgtgac tagctatgcc accttccgtc
    52801 aggacagtga gttttatcag gggatggcct ttgacttcct tttcttagat gaggctcagg
    52861 tcatgaaaaa tgcccagacc aagattgccc agaccttgag acaatttgtg gtgccgtcgg
    52921 tctttgcctt gtcaggaact ccgattgaaa accatctggg tgagttgtgg tctattttcc
    52981 aaatcgtcat gcccggactt ttgccaagca agaaagaatt tatgaaattg ccagcagagc
    53041 gagtggctca gtttatcaag cctttcgtga tgcgacgcaa gaaagaagaa gttctgactg
    53101 aattgccaga cttgattgag gtggtttata aaaatgaact ggaagaccag caaaaggcta
    53161 tttaccttgc ccagttacaa cagatgcgag accatctggc tcaagtgtca gaacaggaat
    53221 ttcagcgaag tcgtgtggaa attttatctg gtttgatgcg cttgcgtcaa atatgtgaca
    53281 ctcctgccct gtttatggaa gattatcagg gagccagcgg caaactagat agtctccgag
    53341 atctactggt acaggtggca gacggcggac accgtgtctt gattttctct cagttcaagg
    53401 gaatgttgga aaaaattgaa caagaactgc cagacttggg cctgacatcc tttaaaatta
    53461 cgggtttaac cccagccaag gaaagacaag acatgaccaa ggcctttaac caaggagaaa
    53521 gagatgcctt tctgatttcc ctcaaggctg gtggggtcgg tctgaacctg acaggtgcag
    53581 atacagtgat cttggttgac ctttggtgga atcctgcggt ggaagcgcaa gccattggcc
    53641 gtgcccatcg gatgggtcag gaagaaacgg ttgaggtcta tcgcttggtg accaagggaa
    53701 ccattgaaga aaaaattcag gaactccaag aacaaaagaa acatctggtg tcacaagtat
    53761 tggatggcac agagtcacgt ggtagtctga ccctagcaga aattagagaa attttgggaa
    53821 tttctgaagc cagcacttga aaaatcaaga caatacgcta taatgaagtg ttgaaaggaa
    53881 atagaaaatg aaacaagaac gatttccatt ggtgtcagat gacgaggtca tgttgactga
    53941 aatgccagtc atgaatctct atgatgagtc tgatctgatc agtaatatca agggtgagta
    54001 tcgagataaa aattatttag aatgggctcc tattactgaa gaaaaaccag taaaaccgat
    54061 tgaaaagcaa gtcgaaaaac ctaaaaaggc tcctttaggg gttaaaaaag aaggaaagag
    54121 ctatgcggag gtggcgcgtg aagaagcgcg tgcggacttg aaaaagaaac gctctgctaa
    54181 ctacctaact caggatttca gccttgcgag acgtcattct cagcccagtc tagttagaca
    54241 gggcaatcaa ccgacaactc ctttccaaaa ggaaaatcct ggtgaatttg tcaaatatag
    54301 ccaaaaattg acccagtctc attatatctt ggcggaagaa gttcattcta tccctaccaa
    54361 gaatgaagaa gtgtcagcac ctgctccaaa gaaaaacaat tatgattttc taaagaagag
    54421 ccaaatctac aataaaaaaa gtaaacaaac agaacaagaa cgtcgggttg cccaagagtt
    54481 gaatctgacc agaatgacag aataggggag aaaacatgtc aaagacatat cattttatcg
    54541 gaattaaggg atcagggatg agtgccttgg ccttgatgtt gcaccagatg gggcacaagg
    54601 ttcagggatc agatgttgaa aagtactact ttacccaacg cggtcttgag caggcaggaa
    54661 ttaccattct tccttttgat gaaaagaatc tagacggtga tatggaaatt atcgctggaa
    54721 atgcctttcg tccagataac aacgtcgaaa ttgcctatgc ggaccaaaat ggtatcagct
    54781 acaaacgtta ccatgagttt ctaggtagct ttatgcgtga ctttgttagc atgggagtag
    54841 caggagcaca tggaaaaact tcaacgacag gtatgttgtc tcatgtcttg tctcacatta
    54901 cagataccag cttcttgatt ggagatggga caggtcgtgg ttcggctaat gccaaatatt
    54961 ttgtctttga atctgacgaa tatgagcgtc acttcatgcc ttaccaccca gaatactcta
    55021 ttatcaccaa cattgacttt gaccatccag attatttcac aagtctcgag gatgttttca
    55081 atgcctttaa cgactatgcc aaacaaatta ccaagggtct ttttgtctat ggtgaagatg
    55141 ctgaattgcg taagattacg tctgatgcac caatttatta ttatggtttt gaagctgaag
    55201 gcaatgactt tgtagctagt gatcttcttc gttcaacaac tggttcaacc ttcaccgttc
    55261 atttccgtgg acaaaacttg gggcaattcc acattccaac ctttggtcgt cacaatatca
    55321 tgaatgcgac agccgttatt ggtcttcttt acacagcagg atttgatttg aacttggtgc
    55381 gtgagcactt gaaaacattt gccggtgtta aacgtcgttt cactgagaaa attgtcaatg
    55441 atacagtgat tattgatgac tttgcccatc atccaacaga aattattgcg accttggatg
    55501 cggctcgtca gaaataccca agcaaggaaa ttgtagcagt ctttcaaccg cataccttta
    55561 caagaaccat tgccctgttg gacgactttg cccatgcttt aaaccaagca gatgctgttt
    55621 atctagcgca aatttatggc tcggctcgtg aagtagatca tggtgacgtt aaggtagaag
    55681 acctagccaa taaaatcaac aaaaaacacc aagtgattac tgttgaaaat gtttctccac
    55741 tcctagacca tgacaatgct gtttacgtct ttatgggagc aggagacatc caaacctatg
    55801 aatactcatt tgagcgtctc ttgtctaact tgacaagcaa tgttcaatag gatgttccca
    55861 tggaaattcc aattaagatc attcaggcaa gcaagtttga tttgcctgag ataggggcac
    55921 ttcagacctc gtcttttcca gctgaaaagc agcaactttc ccatatttta gaagaaagta
    55981 tccgtaagtg tgcggatacc tttcttctag ctagggatga aaatcaactt ttaggctata
    56041 ttttatcaag tccccagtca gacaatccgc aatgtctaaa agtacattct ttagtcatcg
    56101 agtctgacca tcagagacag ggcctgggaa cacttcttct tgcagccttg aaagaggtgg
    56161 cagttgagct ggattacaaa gggattcgtt tggagagtcc tgatgagctg ctttcctatt
    56221 ttgaaatgaa cggttttgtt gatgaagaag aaattttgct ctatgcaact agccagggct
    56281 atagtatgat ttggtttaat cccttttatc tggaggaaca atgaaaatca gacaagcaag
    56341 attagaagat ttggatcgga ttgttgaact tgaatttgaa aatttttcgg tcgaagaagc
    56401 cattcctcct tctgtctttg aagcacattt gagagaaatt cagacctctt ttctggttgc
    56461 tgaaaaagaa ggaagaatca tgggttatat cgaaggacca gttggcctgc accgccatct
    56521 gcaagaccag tcttttacag aagaaataaa agactatagt catgagcctg gtggttatat
    56581 ctctgtgacc tgtctgtcta ttgccaagga ggcacaggga ctcggactgg gtcagaaatt
    56641 gctgacagcc ttgaaagaag ttgctcttga agatgagaga gatggcatta atctaacctg
    56701 tcatgactat ctcatcgcct attatgaaaa acatggattt gtcaacgaag gccagtccca
    56761 gtcaaccttt gcaggggaaa catggtatga tatggtctgg gaaatgaaaa aataagttag
    56821 gaaaagtatc atagattgct ttttttggac ttttaagata taatattatg gattatcaac
    56881 aaggaggaaa aacttttgag tgaaaagtca agagaagaag agaaattaag ctttaaagag
    56941 cagattctga gagatttaga aaaagtaaaa ggctatgatg aagttctgaa agaagatgag
    57001 gcagtagttc gcactcctgc aaatgaacct tcagctgaag aactcatggc tgattccttg
    57061 tcaacggtag aggagattat gagaaaagct cctaccgtgc ctactcaccc aagtcaaggt
    57121 gtaccagctt ctccagcaga tgagattcaa agagaaactc ctggtgttcc aagtcatcca
    57181 agtcaagatg taccttcttc tccagcggaa gaaagtggat caagaccagg tccaggtcct
    57241 gttagaccta agaaacttga aagagaatac aatgaaaccc caacaagggt agctgtttcc
    57301 tatacgacgg cagagaaaaa agcagaacaa gcaggtccag aaacacctac gcctgctaca
    57361 gaaacagtgg atatcatcag agatacatca cgtcgtagcc gtagagaagg agcaaaaccc
    57421 gttaagccta agaaagagaa gaagtcacat gtgaaagctt ttgtgatttc attccttgta
    57481 ttccttgcct tgctctcagc aggtggttac tttggttacc agtacgtgct agattcctta
    57541 ttacctatcg atgctaattc taagaaatat gtgacggttg gaattccaga aggttcaaac
    57601 gttcaagaaa tcggtacgac gcttgaaaaa gctggtttgg taaagcatgg tctgattttt
    57661 agtttttatg ccaagtataa aaattatacc gacttgaaag caggttacta caatttgcaa
    57721 aagagtatga gtacagaaga cttactcaaa gagttgcaaa aaggtggaac agatgaaccg
    57781 caagaacctg tacttgcgac tttgacaatt ccagaaggtt ataccttgga tcagattgct
    57841 caaactgtgg gtcaattgca aggtgacttc aaagagtctt tgacagcgga ggctttcttg
    57901 gctaaagttc aagatgagac gtttatcagt caagcagtag cgaaatatcc tactttactg
    57961 gaaagtttgc ctgtaaaaga cagcggtgcg cgttatcgtt tggaaggata ccttttccca
    58021 gctacatact ctatcaagga aagcacaact attgagagct tgattgatga gatgttagct
    58081 gctatggata agaacctatc tccttactat agtactatca aatctaaaaa cttgactgtc
    58141 aatgagttgt tgaccattgc ttccttggtc gaaaaagaag gtgccaagac agaagatcgt
    58201 aagctcattg caggtgtatt ctacaatcgt ttgaatcgtg atatgccact tcaaagtaat
    58261 attgcaatct tgtatgccca aggaaaactg gggcaaaata tcagtctagc tgaggatgtt
    58321 gcgattgata ccaacattga ttcaccttat aatgtttata aaaatgtagg tctcatgcct
    58381 ggtccagtcg atagtccaag tctggatgcg attgagtcaa gcatcaatca aactaagagc
    58441 gataacctct actttgtagc agatgtcaca gaaggcaagg tctactatgc taacaatcaa
    58501 gaagaccacg accgcaatgt cgctgaacat gtcaacagca aattaaacta aacaaactaa
    58561 aattatgtga tacttcacat aattttcttt agaaaatatt atcagaagaa agttgagaaa
    58621 aatggcagaa aaaacatatc ctatgaccct tgaggaaaag gaaaaacttg aaaaagaatt
    58681 agaagaattg aaattggttc gtcgaccaga agtggtagaa cgcattaaga ttgcccgttc
    58741 atacggtgac ctttcagaaa acagtgagta cgaagcagct aaggatgaac aagcctttgt
    58801 cgaaggacaa atctctagct tagaaacaaa aatccgctat gctgaaatcg tcaatagcga
    58861 cgcagttgcc caggacgaag tagcgattgg taaaacagtc accatccaag aaattggtga
    58921 ggacgaagaa gaagtttata ttatcgtagg ttcagctggt gcggatgcct ttgcaggtaa
    58981 ggtttcaaat gaaagcccaa ttgggcaggc cttgattggc aagaaaacag gtgatacagc
    59041 aaccattgaa acgcctgttg gtagctatga tgtaaaaatc ttgaaggttg aaaaaacagc
    59101 ctaaaaacag aaaaaggagt ggggaggcga tgtgcttcac tcactccttt ttccattttg
    59161 ctactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttgccgtatg tatggttact
    59221 gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg cggctagttt
    59281 cctagtttgc tctttgattt ttattgagta ttagatgtat agtgcattga agttaaaata
    59341 gtacactttg atttctaaga cattgttaga aattagatta aattccttaa tcgatttgcg
    59401 catgttttat ttcatttcac tatactttag attggaaaat aaggaatcac ataaatcgca
    59461 agaaataggg aatctgattc gtgtttataa ccgttcaaaa agagaagagg ctgaaagtga
    59521 gccacttaat ctttatgtcg aagatgaaaa gggcaatctc ctggcaggtt tgatagcaga
    59581 gacttttgga aattggctag aaatcgagta tttgtttgta aaagaggaac tgcgagggca
    59641 aggaatcggt tcaaaactat tgcaacaggc agaaagtgaa gctaagaatc gaaattgttg
    59701 ttttgccttt gtcaatactt accagttcca ggcaccggac ttttatcaga aacatggcta
    59761 caaggaagtt ttttctttgc aagactatct ctacattagg caaagatatt attaccaaaa
    59821 gaatctgtaa ggtgagtatg gaggtataat acaaagagag gctcgcacat aagtatggag
    59881 gtggactgat gtatatgagt aactattgat aaataccagt tataacctag aaattgtgtc
    59941 tttctagacg atattgagga caatataatc gtagccccaa aattaggaat caagacttat
    60001 caggttaaga aaagaagtga tgttgttgat attttaaaca atttatttaa actaaaaact
    60061 ctctatctat taatccgaga ttcccctctc ggattttgtg ttgctttcat taaatttaca
    60121 ggtaagactt gctagcttca gtaaatatac acaagttttt cagaatagag tgaaatccaa
    60181 aagaaaatgg aaataatgaa agctcttgta ggcataagaa aacacctctg tgttatactt
    60241 gttgttcaac cacaaacaca agaaaggcac agaaatgcaa gacaattata ctacaaaagg
    60301 taaacatttg acaatcgata gccgtcgctt aatcgaaaga tggaaaaaag aaggaaaatc
    60361 aaatagagaa attgcctctc tacttggaaa agctcctcaa actatccaca ctaaaatcaa
    60421 gcgtaggaca gtccgaaaat gtcttggaaa agggcgcttc aaagaggttt attctgccga
    60481 ctatgctcaa cagtcttatg aaaataatcg caagcactcg gtcaagagat caagcgtgac
    60541 caaggaacta aaggaaaaga ttctccacta tcataaccaa aaatttttgc ctgaaatgat
    60601 ggttatggct aaaggggtta acgtgggaat ttcaaccatt tactattgga ttcatcatgg
    60661 aaaattgggg taaagcaagc aggatctgct ttaccctaga aaaggaaaag cgcttaagaa
    60721 acaggccagc accaacttta aacctgctgg tcaatccatc gaacagcggc ctgaagctat
    60781 caattttcgc ttggagaatg ggcattatga gattgatacg gttctactta cgagagcgaa
    60841 aaactactgc ttgcttgtct tgacggatcg aaaaagtaga catcagatca tccgattgat
    60901 tccaaataaa agtgctgagg tggtcaatca ggctctaaaa ctcatcttaa aacaacacaa
    60961 gattctttcc atcacggcag ataatggaac ggaattcaat cgcttgtcta atgtattttc
    61021 tgaggagcac atttattatg cacatcccta tgcctcttgg gaaaggggaa ctaatgagaa
    61081 tcacaacagg ctcattcgta gatggttacc taagggaacc aagaaaatga ctcccaaaga
    61141 agtcgcattc atcgaaaagt ggattaacaa ctatcctaaa aaatgcttgg actacaagtc
    61201 acccagagaa gacttctgga tggctaactt gaacttgaaa tttagcaaaa tggaaataat
    61261 ttttattaaa cgcttccaat agcggttaaa agttgacaaa tttaaatttt aggactacac
    61321 taagtaagta aattttattt taaaggagaa tttgttatga atttaacatt tttaggctta
    61381 tgtattgcct gtatgggcgt atctgtcggt gaaggtttat tgatgaatgg actgtttaaa
    61441 tcagtagcac gccaaccaga tatgctttct gagtttcgta gtttgatgtt tttaggtgtt
    61501 gcctttattg aaggaacttt ctttgtaact cttgtcttct catttattat caaataaata
    61561 catggaacga gaagaaaagg gaggatttta gatggaagaa agtattaatc caatcatctc
    61621 tattggtcct gttatcttca atctgactat gttagccatg actttgttga ttgtgggagt
    61681 tatttttgtc tttatttatt gggcaagccg caatatgacc ttgaaaccca aaggaaagca
    61741 aaatgtactt gagtatgtct atgactttgt tattggattt acagaaccta acattggttc
    61801 gcgctacatg aaagattact cactcttttt cctttgttta ttccttttca tggtgattgc
    61861 caataacctt ggcttaatga caaagcttca aacgatcgat gggactaact ggtggagttc
    61921 gccaaccgct aatttacagt atgacttaac cttatctttt cttgtcattt tgttgacaca
    61981 tatagaaagc gttcgtcgtc gtggatttaa aaaaagtata aaatctttta tgagtcctgt
    62041 ttttgtcata ccgatgaata tcttggaaga atttacaaac ttcttatctt tggctttgcg
    62101 gatttttggg aatatctttg caggagaggt catgacgagt ttgttacttc ttctttccca
    62161 ccaaactatt tattggtatc cagtagcctt tggagctaat ttggcttgga ctgcattttc
    62221 tgtctttatt tcctgcatcc aagcttatgt ttttactctt ttgacatctg tgtatttagg
    62281 gaataagatt aatattgaag aggaatagaa aggagtaact gatgcacgta acagtaggtg
    62341 aattaattgg taattttatt ttaatcactg gctcttttat tcttttgcta gtcttgatta
    62401 aaaaatttgc atggtctaat attacaggca ttttcgaaga aagagctgaa aaaattgctt
    62461 cagatattga cagagctgaa gaagcccgtc aaaaagcaga agtattggct caaaaacgcg
    62521 aagatgaatt ggctggtagc cgtaaagaag ctaagacaat cattgaaaat gcaaaggaaa
    62581 cagctgagca aagtaaggct aatatcttag cagatgctaa actagaagca ggacacttaa
    62641 aagaaaaagc caatcaagaa attgctcaaa ataaagtaga agctttacag agtgttaagg
    62701 gtgaggtcgc agatttgacc atcagcttag ctggtaaaat catctcacaa aaccttgaca
    62761 gtcatgccca taaagcactc attgatcagt atatcgatca gctaggagaa gcttaatgga
    62821 caagaaaaca gtaaaggtaa ttgaaaaata cagcatgcct tttgtccaat tggtacttga
    62881 aaaaggagaa gaagaccgta tcttttcaga cttgactcaa atcaagcaag ttgttgaaaa
    62941 aacaggtctg ccttcttttt taaaacaagt ggcagtagac gagtcggata aggaaaaaac
    63001 aattgctttt ttccaagatt ctgtgtcgcc tttattacaa aactttatcc aggttctggc
    63061 ctacaatcac agagcaaatc ttttttatga tgtgcttgta gattgcttga accgacttga
    63121 aaaagaaaca aatcgatttg aagtgacgat tacgtctgct catcctctaa ctgatgaaca
    63181 gaagactcgt ttgctccctt tgattgagaa aaaaatgtct ctgaaagtaa ggagtgtaaa
    63241 agaacaaatc gatgaaagtc tcattggtgg ttttgtcatt tttgccaatc acaagacaat
    63301 tgatgtgagt attaaacaac aacttaaagt tgttaaagaa aatttgaaat agaaagtggt
    63361 gttcttttgg caattaacgc acaagaaatc agcgctttaa ttaagcaaca aattgaaaat
    63421 ttcaaaccca attttgatgt gactgaaaca ggtgttgtaa cctatatcgg ggacggtatc
    63481 gcgcgtgctc atggccttga aaatgtcatg agtggagagt tgttgaattt tgaaaacggc
    63541 tcttatggta tggctcaaaa cttggagtca acagacgttg gtattatcat cctaggtgac
    63601 tttacagata tccgtgaagg cgatacaatc cgccgtacag ggaaaatcat ggaagtccct
    63661 gtaggtgaaa gtctgattgg tcgtgttgtg gatccgcttg gtcgtccagt tgacggtctt
    63721 ggagaaatcc acactgataa aactcgtcca gtagaagcac cagctcctgg tgttatgcaa
    63781 cgtaagtctg tttcagaacc attgcaaact ggtttgaaag ctattgacgc ccttgtaccg
    63841 attggtcgtg gtcaacgtga gttgattatc ggtgaccgtc agacagggaa aacaaccatt
    63901 gcgattgata caatcttgaa ccaaaaagat caagatatga tctgtatcta cgtcgcgatt
    63961 ggacaaaaag aatcaacagt tcgtacgcaa gtagaaacac ttcgtcagta cggtgccttg
    64021 gactacacaa tcgttgtgac agcctctgct tcacaaccat ctccattgct cttcctagct
    64081 ccttatactg gggttgctat ggcggaagaa tttatgtatc aaggtaagca tgttttgatt
    64141 gtatacgatg atctatcaaa acaagcggta gcttatcgtg aactatcgct cttgcttcgt
    64201 cgtcctccag gtcgtgaagc cttcccaggg gatgttttct atctccacag ccgtttgctt
    64261 gagcgctcag ctaaagtttc tgatgaactt ggtggtggat caattacagc cctaccattt
    64321 atcgagacac aagcaggaga tatctcagcc tatatcgcaa ccaacgtgat ttctatcact
    64381 gatggacaaa tcttccttgg cgatggcctc ttcaatgcag gtattcgtcc agccatcgat
    64441 gcgggttcat ctgtatctcg tgtaggtggt tctgcacaaa tcaaagccat gaagaaggtt
    64501 gctggtacac ttcgtatcga ccttgcttca taccgtgagt tggaagcctt tactaagttt
    64561 ggttctgact tggacgcagc aacacaggct aagttgaacc gtggacgtcg taccgttgag
    64621 gtcttgaaac aacctgttca caaaccatta cctgttgaga aacaagtaac cattctttat
    64681 gctttgacac atggtttctt ggatactgtt ccagtagatg atattgttcg tttcgaggaa
    64741 gagttccatg ccttctttga tgctcaacat ccagagattt tggaaaccat tcgtgataca
    64801 aaagacttgc cagaagaagc agtcttggat gctgcgatta cagagtttct caatcaatct
    64861 agcttccaat aagaatagag gtgtcagatg gcagtgtctc taaatgatat taaaacaaaa
    64921 atcgcctcaa caaaaaatac gagtcaaatc actaatgcca tgcaaatggt atcggctgct
    64981 aagttaggtc gctctgaaga agctgctcgc aacttccaag tttacgctca gaaagtgcgt
    65041 aaacttttga cagatatcct tcatggtaat ggagctggtg cttcaactaa tccgatgttg
    65101 attagccgtt ctgtgaagaa gacaggctat atcgttatca cttcagaccg cggtttggtt
    65161 ggaggttata attcctctat tttgaaagct gttatggagt tgaaagaaga ataccaccca
    65221 gacggtaaag gttttgaaat gatctgtatc ggtgggatgg gagctgattt ctttaaggct
    65281 cgcggtattc aaccacttta tgaattacgt ggcttggcag accaacctag ctttgatcaa
    65341 gttcgtaaga ttatttcaaa aactgttgaa atgtaccaaa atgaactctt tgatgagctt
    65401 tatgtttgct acaaccacca tgtcaatacg ctaaccagtc aaatgcgtgt ggaacaaatg
    65461 cttccgattg ttgacttgga tccaaatgaa gcggatgaag agtacagctt gacttttgaa
    65521 ttggaaacca gccgagaaga aattctggag cagttgttgc ctcagtttgc agaaagtatg
    65581 atttacggtg ccattatcga tgccaagaca gctgagaatg ctgcgggtat gacagccatg
    65641 caaacagcga cagataatgc taagaaagtc atcaatgatt tgacaattca gtataaccgt
    65701 gccagacagg cggcgattac acaagaaatt acagaaatcg tagcaggtgc tagtgcctta
    65761 gaataggccc tagtccagct cgtatgaaaa tgaacttagg acctagttga gctaggaacc
    65821 gacagtatct tatatagaat aggagaagga gatgagttca ggtaaaattg ctcaggttat
    65881 cggtcccgtt gtagacgttt tgtttgcagc aggggaaaaa cttcctgaga ttaacaatgc
    65941 acttgtcgtc tacaaaaatg acgaaagaaa aacaaaaatc gtccttgaag tagccttgga
    66001 gttaggagat ggtatggttc gtactatcgc catggaatca acagatgggt tgactcgtgg
    66061 aatggaagta ttggacacag gtcgtccaat ctctgtacca gtaggtaaag aaactttggg
    66121 acgtgtcttc aacgttttgg gagataccat tgatttggaa gctcctttta cagaagacgc
    66181 agagcgtcag ccaattcata aaaaagctcc aacttttgat gagttgtcta cctcttctga
    66241 aatccttgaa acagggatca aggttattga ccttcttgcc ccttacctta aaggtggtaa
    66301 agttggactt ttcggtggtg ccggagttgg taaaaccgtc ttaatccaag aattgattca
    66361 caacattgcc caagagcacg gtggtatttc agtatttgct ggtgttgggg aacgtactcg
    66421 tgaggggaat gacctttact gggaaatgaa agaatcaggc gttatcgaga aaacagccat
    66481 ggtctttggt cagatgaatg agccaccagg agcacgtatg cgtgttgccc ttactggttt
    66541 gacaatcgct gaatacttcc gtgatgtgga aggccaagac gtgcttctct ttatcgataa
    66601 tatcttccgt ttcactcagg ctggttcaga agtatctgcc cttttgggtc gtatgccatc
    66661 agccgttggt taccaaccaa cacttgctac ggaaatgggt caattgcaag aacgtatcac
    66721 atcaaccaag aagggttctg taacctctat ccaggctatc tatgtgccag cggatgacta
    66781 tactgaccca gcgccagcaa cagccttcgc tcacttggat tcaacaacaa acttggaacg
    66841 taagttggta caattgggta tctacccagc cgttgaccca cttgcttcaa gctcacgtgc
    66901 cttggcacct gaaatcgttg gagaagagca ctatgcagtt gctgctgaag taaaacgtgt
    66961 ccttcaacgt taccatgaat tgcaagatat cattgctatc cttggtatgg atgagctttc
    67021 tgatgaagaa aagaccttgg ttgctcgcgc ccgtcgtatc cagttcttct tgtcacaaaa
    67081 cttcaacgtt gcggaacaat ttactggtca gccaggttct tatgttccag ttgctgaaac
    67141 tgtacgtggc tttaaggaaa tccttgatgg taaatacgac cacttgccag aagatgcctt
    67201 ccgtggtgta ggttctatcg aagatgtgat tgcaaaagct gaaaaaatgg gattttaaga
    67261 ggtgatctat ggctcagtta actgtccaga tcgtgacacc agatggtctc gtctatgatc
    67321 accatgccag ctatgtatcg gttcgaactc tggatggtga gatggggatc ttgccacgac
    67381 atgaaaatat gattgcagtt ttagcagttg atgaagtaaa ggtaaaacgt atcgatgata
    67441 aagatcacgt gaactggatt gcagtaaacg gtggtgttat tgaaattgcc aatgatatga
    67501 tcacaatcgt cgctgactct gcagaacgtg ctcgtgatat cgatatcagt cgtgcagaac
    67561 gtgccaaact tcgtgcagaa cgtgcaattg aagaagcaca agacaaacat ttgattgacc
    67621 aagaacgtcg tgctaagatt gctttgcaac gtgctattaa ccgtattaat gtcggaaata
    67681 gactataaga aaaaatgaac ttgaaaatac caagttcatt ttttatggtg ttttaaggag
    67741 caaaacggat gcagactgct tcgggaacat ggaagtcgtt ggagagttct gctagacgac
    67801 cattgtcaca attacgttta aagacagttg cattgtcaga gtcttgatgg acaacaatga
    67861 gaaatttttg gtcgggtgtc aaatcaaaat cacgtggagt ctgaccatgc gttggaacga
    67921 tttctaataa ctctaagcta ccgtccgcaa ggatggtata tactgcgata gaatcatggc
    67981 cacggttaga agcgtagagg tatttaccgt ctttagagag atgaatagca gcggttccat
    68041 taaagccttc gtaagcttcc ggtaaagttg aaatgacctg catacgttca aattcgccaa
    68101 cgccatcgta gattaaaact tcgatagtac tattgagttc acaaatgaga taagcgattt
    68161 tatagtggtt atggaaaatg atatggcgtg agcctgctcc tggcttgctg tgataggtat
    68221 agagcttaga taattttcct tcttgatcga ggtcataggt gatgacttgg tcagttccca
    68281 agtcgcaggt cactagatag tggtcaggtg ttaaatctgt atagtgaaca tggggggaag
    68341 cttgattttc atgtggacct tggccactgt gttgatccat atcactaagt agaagactac
    68401 catcttcctg gcgtttataa acaaggactt gtcccttgtg atagttggct gcgtaaacca
    68461 aatcacgctt ttcatcgaca gcaacataac agtggggagc tccttcttca acaacatgat
    68521 ttaacacagt cccgtcagtt tgataggctg caattccccc cttatcgtct tggctaccaa
    68581 cagtgtataa atgttggtgc tggtcaaagg caaggtaggt tggacttggc tcagctgcaa
    68641 aaagttctag atttgaaagc tgaccagttt ctgtatcaaa gtctgccttg taaatccctt
    68701 gagaagtacg acgtgtataa gttccaaaat aaacagtttc tttcattact atacctctgt
    68761 gtaaagataa gactattata tcacaaaaac aagcaaatta aagacattca attagatgta
    68821 agcactttaa aaaagagtta ttttgtttca aaaatggtat aatgagagaa caatagaaag
    68881 gaagtattta tggagcaaaa agagaaacat tttagcctat cttggttttt caagtggttt
    68941 ttagataaca aggcaattac ggtattttta gtaaccttat tattgggatt gaatcttttt
    69001 attttaagta agattagttt tctattttca cctgttttag actttttagc agttgtgatg
    69061 ttgccagtca ttttgtctgg tttgttatat tatttgttga atcctattgt tgattggatg
    69121 gagaagcata aggttaatcg tgttatagct atcactattg tctttgttat catcgctctc
    69181 tttatcattt ggggcttggc agtcgccatt ccaaatctgc aacgtcaggt tttgaccttt
    69241 gcaagaaacg ttcctgttta cttagaagat atagatagga ttgttaatgg attggtagcc
    69301 cagcatctgc cagatgattt cagacctcaa ttagagcaag ttttgaccaa tttttctagc
    69361 caggctacag ttttggcaag taaggtttca tctcaggcag tcaactgggt gagtgccttt
    69421 attagcgggg cttctcaagt gattgttgcc ttgattatcg ttcctttcat gctcttttat
    69481 ctcttgcgtg atgggaaagg cttgcgtaac tatttgaccc aattcattcc aagaaaattg
    69541 aaggaacctg ttggacaagt tctatcagat gtgaatcaac agttgtccaa ctatgttcga
    69601 gggcaagtga cagtggctat tattgtagca gtaatgttta tcatcttctt caagattatt
    69661 ggtctacgct atgcggttac gctgggggtt actgctggta ttttaaatct ggtcccttat
    69721 cttggtagct ttctagccat gcttcctgcc ctagtattgg gtttgattgc tggtccagtc
    69781 atgcttttga aagtagtgat tgtctttatt gtagaacaaa ctattgaagg ccgttttgtc
    69841 tctccattga ttttgggaag tcaattaaac atccacccta ttaatgttct ctttgttttg
    69901 ttaacttcag gatctatgtt tggtatctgg ggagttttac ttggtattcc agtttatgcc
    69961 tctgctaagg ttgtcatttc agccattttc gaatggtata aggtagtcag tggtctatat
    70021 gaattagagg gtgaggaagt caagagtgaa caatagtcaa cagatgttac aggctttgga
    70081 ggagcaagat ttaactaagg ctgagcatta tttcgccaaa gctttagaaa atgattcaag
    70141 tgatcttctg tatgagttgg caacttatct tgaagggatt ggtttctatc ctcaggccaa
    70201 ggaaatttac ctgaaaattg tagaggattt tccagaggtt catcttaatc tagctgcaat
    70261 tgctagcgag gatggtcaaa tagaagaagc ctttacctat cttgaggaaa tccaagctga
    70321 cagtgactgg tatgtctcgt ctttggtttt gaaggcagac ctttaccagc tggaaggttt
    70381 gacagatgtg gcacgtgaga aattattgga ggccttgacc tactcagagg attctctctt
    70441 gatattgggt ttggcagagt tggatagtga gttggaaaat taccaagcgg ctattcaagc
    70501 ctatgcccag ttagataatc gctcgattta tgagcaaacg ggcatttcca cctatcaacg
    70561 aattggcttt gcctatgctc agttagggaa atttgaaacg gctactgagt ttttagaaaa
    70621 agccctggag ttagaatacg atgacttaac agcttttgag ttggccagtc tttattttga
    70681 tcaagaagaa tatcaaaaag ccaccctcta ctttaagcag cttgatacca tttctcctga
    70741 ctttgaaggc tatgagtatg ggtacagtca ggctttacat aaggaacatc aagttcaaga
    70801 agccctgcgt atcgctaagc aaggattaga gaaaaatccc tttgaaactc gcctcttgct
    70861 agctgcttca caattttctt atgaattgca tgatgctagt ggtgcagaaa attatctcct
    70921 tactgcaaaa gaagacgctg aggatacaga agaaatcttg cttcgtttag ccactattta
    70981 tctggagcag gagcgttatg aggatattct agacttgcag agtgaggagc cagaaaatct
    71041 tttgaccaag tggatgattg ctcgttctta tcaagaaatg gacgatttgg atactgctta
    71101 tgagcattat caagagttga caggagattt gaaggacaat ccagaatttc tggaacacta
    71161 tatctatctc ttgcgtgaat tgggacattt tgaagaagca aaagtccatg ctcacactta
    71221 cttaaaactg gttccagatg atgtgcaaat gcaagaactg tttgagagat tgtaagaatg
    71281 tttaaacata tagaactgta gtttatctct tttgatagct gcggttttta tttgtatatg
    71341 gtagaatctt tttacaaaaa tacttggtaa tcttgtttat tcatgctata ataggaacaa
    71401 ttacttttag gaggtgcagt atgtcttatt tatttgagat attaccgagt ttactgaatg
    71461 gtgcgagcac gactgtacag gtctttgcac tggtcttgct attttcgatt ccgttgggcg
    71521 ttttgattgc ctttgccttg caagtccatt ggaagcccct ccattatctg attaacattt
    71581 acatctgggt tatgcgagga acccccttac tcttgcaact gatttttatc tattatgtgc
    71641 tcccaagtat tgggattcgt ttagaccgcc ttcctgcagc tattattgcc tttgttctca
    71701 actatgcagc ttactttgca gaaattttcc gtgggggaat tgacactatt ccaagaggac
    71761 agtatgaggc tgccaaggtc ttgaagttta gcccttttga cagagtgcgc tatattatct
    71821 tgccccaagt gaccaagatc gttcttccta gtgtctttaa tgaagttatg agtttggtca
    71881 aggatacttc tttggtctat gctctcggaa tttcagacct tatcttggct agtcgaacag
    71941 ctgctaaccg cgatgctagt ctggttccta tgttcttggc aggagccatt tacttgattt
    72001 taattggggt tgtgacaatt atttccaaaa aagttgagaa gaagtatagt tattatagat
    72061 agggggcagc tatgttagaa ttacgaaata tcaataaagt ctttggagac aaacaaatcc
    72121 tgtctgattt cagtctaagt attcctgaaa agcaaatcct ggctatcgtt ggaccttctg
    72181 gtggaggtaa gacaactctt ttacgtatgc ttgcaggtct tgaaaccatt gattcagggc
    72241 aaatctttta taatggacaa cctttagagc tagatgaatt gcagaagcgc aacctactgg
    72301 gatttgtctt ccaagatttt caactatttc ctcatctatc agttctggaa aatttgactt
    72361 tatcgcctgt gaagaccatg ggaatgaagc aggaagaggc tgagaagaag gcgagtgaac
    72421 tcttggaaca gttaggacta ggaggacacg cagaggccta tcctttctca ctatctggtg
    72481 ggcaaaagca gcgggtggct ttggcgcgtg ctatgatgat tgacccagaa atcattggct
    72541 acgatgaacc aacttctgcc ctggatccag aattacgttt ggaagtggag aagctaatct
    72601 tgcaaaatag ggaacttggg atgacccaga ttgtggttac ccatgatttg cagtttgctg
    72661 aaaatatcgc agatgtatta ttgaaagtag aacctaaata ggaggaaaaa tggatgaaaa
    72721 aatggatgct tgtattagtc agtctgatga ctgctttgtt cttagtagct tgtgggaaaa
    72781 attctagcga aactagtgga gataattggt caaagtacca gtctaacaag tctattacta
    72841 ttggatttga tagtactttt gttccaatgg gatttgctca gaaagatggt tcttatgcag
    72901 gatttgatat tgatttagct acagctgttt ttgaaaaata cggaatcacg gtaaattggc
    72961 aaccgattga ttgggatttg aaagaagctg aattgacaaa aggaacgatt gatctgattt
    73021 ggaatggcta ttccgctaca gacgaacgcc gtgaaaaggt ggctttcagt aactcatata
    73081 tgaagaatga gcaggtattg gttacgaaga aatcatctgg tatcacgact gcaaaggata
    73141 tgactggaaa gacattagga gctcaagctg gttcatctgg ttatgcggac tttgaagcaa
    73201 atccagaact tttgaagaat attgtcgcta ataaggaagc gaatcaatac caaaccttta
    73261 atgaagcctt gattgatttg aaaaacgatc gaattgatgg tctattgatt gaccgtgtct
    73321 atgcaaacta ttatttagaa gcagaaggtg ttttaaacga ttataatgtc tttacagttg
    73381 gactagaaac agaagctttt gcggttggag cccgtaagga agatacaacc ttggttaaga
    73441 agataaatga agctttttct agtctttaca aggacggcaa gttccaagaa atcagccaaa
    73501 aatggtttgg agaagatgta gcaaccaaag aagtaaaaga aggacagtaa gataaaatag
    73561 tggctgaaac tgcgttttga ttagcaaaac gtagtttttt tgtaatctag gaaaacgata
    73621 atagcgattg aatctggata att
//
LOCUS       DAVLRT010000002        67951 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLRT010000002 DAVLRT010000000
VERSION     DAVLRT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.002"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..1450)
                     /gene="pfbA"
                     /locus_tag="VXM85_000078"
     CDS             complement(<1..1450)
                     /gene="pfbA"
                     /locus_tag="VXM85_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1344738.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYL"
     gene            complement(2614..3840)
                     /locus_tag="VXM85_000079"
     CDS             complement(2614..3840)
                     /locus_tag="VXM85_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1344739.1"
                     /translation="MPNYNIPFSPPDITEAEITEVVDTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSIYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAASRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(3883..4575)
                     /locus_tag="VXM85_000080"
     CDS             complement(3883..4575)
                     /locus_tag="VXM85_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1344740.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(4748..6499)
                     /locus_tag="VXM85_000081"
     CDS             complement(4748..6499)
                     /locus_tag="VXM85_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344741.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATMVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQETSDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGV
                     TYHKMYSLQAGAMADTL"
     gene            complement(6489..8234)
                     /locus_tag="VXM85_000082"
     CDS             complement(6489..8234)
                     /locus_tag="VXM85_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344742.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLLAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGTI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(8440..8736)
                     /locus_tag="VXM85_000083"
     CDS             complement(8440..8736)
                     /locus_tag="VXM85_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1344743.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(8889..9346)
                     /locus_tag="VXM85_000084"
                     /pseudo
     CDS             complement(8889..9346)
                     /locus_tag="VXM85_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            9480..10307
                     /gene="xth"
                     /locus_tag="VXM85_000085"
     CDS             9480..10307
                     /gene="xth"
                     /locus_tag="VXM85_000085"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1344744.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            10319..10456
                     /locus_tag="VXM85_000086"
     CDS             10319..10456
                     /locus_tag="VXM85_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1344745.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLLKSV
                     N"
     gene            10567..10695
                     /locus_tag="VXM85_000087"
     CDS             10567..10695
                     /locus_tag="VXM85_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV1344746.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      11078..11173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11573..12154
                     /locus_tag="VXM85_000088"
     CDS             11573..12154
                     /locus_tag="VXM85_000088"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1344747.1"
                     /translation="MKLLEDRILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            12154..13416
                     /locus_tag="VXM85_000089"
     CDS             12154..13416
                     /locus_tag="VXM85_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1344748.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPLDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTRFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(13467..13928)
                     /locus_tag="VXM85_000090"
     CDS             complement(13467..13928)
                     /locus_tag="VXM85_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV1344749.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(13925..14689)
                     /locus_tag="VXM85_000091"
     CDS             complement(13925..14689)
                     /locus_tag="VXM85_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV1344750.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(14870..15274)
                     /locus_tag="VXM85_000092"
     CDS             complement(14870..15274)
                     /locus_tag="VXM85_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1344751.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(15344..16825)
                     /locus_tag="VXM85_000093"
     CDS             complement(15344..16825)
                     /locus_tag="VXM85_000093"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1344752.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(16844..18022)
                     /locus_tag="VXM85_000094"
     CDS             complement(16844..18022)
                     /locus_tag="VXM85_000094"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1344753.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            18187..19194
                     /gene="galR"
                     /locus_tag="VXM85_000095"
     CDS             18187..19194
                     /gene="galR"
                     /locus_tag="VXM85_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1344754.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(19240..20277)
                     /locus_tag="VXM85_000096"
     CDS             complement(19240..20277)
                     /locus_tag="VXM85_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1344755.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(20278..20631)
                     /locus_tag="VXM85_000097"
     CDS             complement(20278..20631)
                     /locus_tag="VXM85_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1344756.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(20875..21765)
                     /locus_tag="VXM85_000098"
     CDS             complement(20875..21765)
                     /locus_tag="VXM85_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1344757.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            21898..22434
                     /locus_tag="VXM85_000099"
     CDS             21898..22434
                     /locus_tag="VXM85_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1344758.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(22600..23406)
                     /locus_tag="VXM85_000100"
     CDS             complement(22600..23406)
                     /locus_tag="VXM85_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1344759.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(23751..25271)
                     /locus_tag="VXM85_000101"
     CDS             complement(23751..25271)
                     /locus_tag="VXM85_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1344760.1"
                     /translation="MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLNGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITENQMSQLNYQVGVEGKSAEQVAKEFLQEQGLLKK"
     gene            complement(25264..25992)
                     /locus_tag="VXM85_000102"
     CDS             complement(25264..25992)
                     /locus_tag="VXM85_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344761.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKILLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVS
                     NPETILKAPATDFVADLFGGSIHD"
     gene            complement(26007..26771)
                     /locus_tag="VXM85_000103"
     CDS             complement(26007..26771)
                     /locus_tag="VXM85_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1344762.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(26775..27218)
                     /locus_tag="VXM85_000104"
     CDS             complement(26775..27218)
                     /locus_tag="VXM85_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1344763.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(27900..28196)
                     /locus_tag="VXM85_000105"
     CDS             complement(27900..28196)
                     /locus_tag="VXM85_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1344764.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(28218..29282)
                     /gene="pepA"
                     /locus_tag="VXM85_000106"
     CDS             complement(28218..29282)
                     /gene="pepA"
                     /locus_tag="VXM85_000106"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1344765.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPYVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            29683..30384
                     /locus_tag="VXM85_000107"
     CDS             29683..30384
                     /locus_tag="VXM85_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEV1344766.1"
                     /translation="MTKTILVTGGTSYISSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNAAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            30807..31766
                     /locus_tag="VXM85_000108"
     CDS             30807..31766
                     /locus_tag="VXM85_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1344767.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            31756..32712
                     /locus_tag="VXM85_000109"
     CDS             31756..32712
                     /locus_tag="VXM85_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1344768.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSFFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            32709..33461
                     /locus_tag="VXM85_000110"
     CDS             32709..33461
                     /locus_tag="VXM85_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1344769.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            33557..34522
                     /locus_tag="VXM85_000111"
     CDS             33557..34522
                     /locus_tag="VXM85_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1344770.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(34747..34989)
                     /gene="yidD"
                     /locus_tag="VXM85_000112"
     CDS             complement(34747..34989)
                     /gene="yidD"
                     /locus_tag="VXM85_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1344771.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(34989..35711)
                     /locus_tag="VXM85_000113"
     CDS             complement(34989..35711)
                     /locus_tag="VXM85_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1344772.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVVNKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(35698..36267)
                     /locus_tag="VXM85_000114"
     CDS             complement(35698..36267)
                     /locus_tag="VXM85_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1344773.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(36286..37014)
                     /locus_tag="VXM85_000115"
     CDS             complement(36286..37014)
                     /locus_tag="VXM85_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1344774.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(37014..37754)
                     /gene="xerD"
                     /locus_tag="VXM85_000116"
     CDS             complement(37014..37754)
                     /gene="xerD"
                     /locus_tag="VXM85_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1344775.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(37745..38206)
                     /locus_tag="VXM85_000117"
     CDS             complement(37745..38206)
                     /locus_tag="VXM85_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1344776.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(38203..38724)
                     /locus_tag="VXM85_000118"
     CDS             complement(38203..38724)
                     /locus_tag="VXM85_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1344777.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(38700..39671)
                     /locus_tag="VXM85_000119"
     CDS             complement(38700..39671)
                     /locus_tag="VXM85_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1344778.1"
                     /translation="MTNKIYEYKDDRDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(39668..40462)
                     /gene="racE"
                     /locus_tag="VXM85_000120"
     CDS             complement(39668..40462)
                     /gene="racE"
                     /locus_tag="VXM85_000120"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1344779.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(40669..40917)
                     /locus_tag="VXM85_000121"
     CDS             complement(40669..40917)
                     /locus_tag="VXM85_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1344780.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(41173..42798)
                     /gene="treC"
                     /locus_tag="VXM85_000122"
     CDS             complement(41173..42798)
                     /gene="treC"
                     /locus_tag="VXM85_000122"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1344781.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMTDYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(42976..44943)
                     /gene="treP"
                     /locus_tag="VXM85_000123"
     CDS             complement(42976..44943)
                     /gene="treP"
                     /locus_tag="VXM85_000123"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1344782.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATNPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            45128..45838
                     /gene="treR"
                     /locus_tag="VXM85_000124"
     CDS             45128..45838
                     /gene="treR"
                     /locus_tag="VXM85_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1344783.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(45871..46674)
                     /locus_tag="VXM85_000125"
     CDS             complement(45871..46674)
                     /locus_tag="VXM85_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1344784.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMNLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(46687..47580)
                     /locus_tag="VXM85_000126"
     CDS             complement(46687..47580)
                     /locus_tag="VXM85_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1344785.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            complement(47592..48452)
                     /locus_tag="VXM85_000127"
     CDS             complement(47592..48452)
                     /locus_tag="VXM85_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1344786.1"
                     /translation="MSQFITHQSLYATREGFVDKLAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQINLIKKVLPKAKTIGILYTQSEPNSVVQKDEAKRLLKEKGFT
                     VVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQVSIKHKVPVFGGSTE
                     MIAVGGLYNYGTNYEELGRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            complement(49019..49945)
                     /locus_tag="VXM85_000128"
     CDS             complement(49019..49945)
                     /locus_tag="VXM85_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344787.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(49956..51023)
                     /locus_tag="VXM85_000129"
     CDS             complement(49956..51023)
                     /locus_tag="VXM85_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344788.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(51032..51958)
                     /locus_tag="VXM85_000130"
     CDS             complement(51032..51958)
                     /locus_tag="VXM85_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1344789.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(51958..53454)
                     /locus_tag="VXM85_000131"
     CDS             complement(51958..53454)
                     /locus_tag="VXM85_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1344790.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(53521..55500)
                     /locus_tag="VXM85_000132"
     CDS             complement(53521..55500)
                     /locus_tag="VXM85_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1344791.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            complement(56186..57379)
                     /locus_tag="VXM85_000133"
     CDS             complement(56186..57379)
                     /locus_tag="VXM85_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1344792.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(57517..58959)
                     /gene="gtfA"
                     /locus_tag="VXM85_000134"
     CDS             complement(57517..58959)
                     /gene="gtfA"
                     /locus_tag="VXM85_000134"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1344793.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTMLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(59259..60092)
                     /locus_tag="VXM85_000135"
     CDS             complement(59259..60092)
                     /locus_tag="VXM85_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1344794.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(60106..60972)
                     /locus_tag="VXM85_000136"
     CDS             complement(60106..60972)
                     /locus_tag="VXM85_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1344795.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAAFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(60986..62245)
                     /locus_tag="VXM85_000137"
     CDS             complement(60986..62245)
                     /locus_tag="VXM85_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1344796.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(62337..64499)
                     /locus_tag="VXM85_000138"
     CDS             complement(62337..64499)
                     /locus_tag="VXM85_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1344797.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHITLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            64607..65467
                     /locus_tag="VXM85_000139"
     CDS             64607..65467
                     /locus_tag="VXM85_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1344798.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIS
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQSPSHTRKEYS
                     QYQLVRKATL"
     gene            65464..66399
                     /locus_tag="VXM85_000140"
     CDS             65464..66399
                     /locus_tag="VXM85_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1344799.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEINSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(66525..66612)
                     /locus_tag="VXM85_000141"
     tRNA            complement(66525..66612)
                     /locus_tag="VXM85_000141"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66576..66578),aa:Ser,seq:gct)
     gene            complement(66619..66692)
                     /locus_tag="VXM85_000142"
     tRNA            complement(66619..66692)
                     /locus_tag="VXM85_000142"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66656..66658),aa:Ile,seq:gat)
     gene            complement(66728..66798)
                     /locus_tag="VXM85_000143"
     tRNA            complement(66728..66798)
                     /locus_tag="VXM85_000143"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66764..66766),aa:Gly,seq:tcc)
     gene            complement(66818..66890)
                     /locus_tag="VXM85_000144"
     tRNA            complement(66818..66890)
                     /locus_tag="VXM85_000144"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66855..66857),aa:Phe,seq:gaa)
     gene            complement(66894..66967)
                     /locus_tag="VXM85_000145"
     tRNA            complement(66894..66967)
                     /locus_tag="VXM85_000145"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66931..66933),aa:Met,seq:cat)
     gene            complement(66978..67067)
                     /locus_tag="VXM85_000146"
     tRNA            complement(66978..67067)
                     /locus_tag="VXM85_000146"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67029..67031),aa:Ser,seq:tga)
     gene            complement(67078..67151)
                     /locus_tag="VXM85_000147"
     tRNA            complement(67078..67151)
                     /locus_tag="VXM85_000147"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67115..67117),aa:Ile,seq:cat)
     gene            complement(67165..67238)
                     /locus_tag="VXM85_000148"
     tRNA            complement(67165..67238)
                     /locus_tag="VXM85_000148"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67202..67204),aa:Met,seq:cat)
     gene            complement(67244..67317)
                     /locus_tag="VXM85_000149"
     tRNA            complement(67244..67317)
                     /locus_tag="VXM85_000149"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67281..67283),aa:Pro,seq:tgg)
     gene            complement(67333..67406)
                     /locus_tag="VXM85_000150"
     tRNA            complement(67333..67406)
                     /locus_tag="VXM85_000150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67370..67372),aa:Arg,seq:acg)
     gene            complement(67416..67501)
                     /locus_tag="VXM85_000151"
     tRNA            complement(67416..67501)
                     /locus_tag="VXM85_000151"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67465..67467),aa:Leu,seq:taa)
     gene            complement(67509..67580)
                     /locus_tag="VXM85_000152"
     tRNA            complement(67509..67580)
                     /locus_tag="VXM85_000152"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67546..67548),aa:Gly,seq:gcc)
     gene            complement(67603..67675)
                     /locus_tag="VXM85_000153"
     tRNA            complement(67603..67675)
                     /locus_tag="VXM85_000153"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67640..67642),aa:Thr,seq:tgt)
     gene            complement(67694..67775)
                     /locus_tag="VXM85_000154"
     tRNA            complement(67694..67775)
                     /locus_tag="VXM85_000154"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67739..67741),aa:Leu,seq:tag)
     gene            complement(67781..67853)
                     /locus_tag="VXM85_000155"
     tRNA            complement(67781..67853)
                     /locus_tag="VXM85_000155"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67818..67820),aa:Lys,seq:ttt)
     gene            complement(67878..67950)
                     /locus_tag="VXM85_000156"
     tRNA            complement(67878..67950)
                     /locus_tag="VXM85_000156"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67915..67917),aa:Asp,seq:gtc)
BASE COUNT        20301 a        15331 c        12636 g        19683 t
ORIGIN      
        1 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
       61 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
      121 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
      181 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
      241 cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
      301 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
      361 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
      421 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
      481 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
      541 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
      601 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
      661 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac
      721 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
      781 ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg
      841 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
      901 aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
      961 ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca ccataatctt
     1021 ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt
     1081 ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt ctgttgtcta
     1141 attgtgaatt ggaacgaata gtaccattag agctgatgct attaacactt aacgaattat
     1201 ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattc tgattaacag
     1261 ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta gccatcggtg
     1321 aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca
     1381 atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca ttaggaacaa
     1441 aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct
     1501 taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc aaattgaatt
     1561 gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta
     1621 ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta tagtagactg
     1681 aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt gactatcccg
     1741 atcgatttgt cctattctta tttcctttta ctatatctac aacctcaaag cagggttttg
     1801 agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat
     1861 ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttatt gatatgttgt
     1921 aaattataaa tgaaagaaca aataccttgt catacaagca ttcgccaggg atatttacct
     1981 taatatcgct catatggcgg attgaaacta aaacactaca atatatttct taaatagacc
     2041 ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga
     2101 atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcag gagatctaat
     2161 gatgtatagc aagcatcact aaacatatgg gatatttgcg ttattataat atcaacatgc
     2221 tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa
     2281 caatattctg aaaacaaaaa aaagacgcta gtttccacaa tctcactcat ggaaactcac
     2341 gtctttcaaa aaatagggtg aattgaaaac tagaacaaga cactagattt tctaaatgat
     2401 gtctagtaag gattctaaat gctataccaa attgctcaca tcttatttca aaccacggga
     2461 ctcccacaag aaaggaaaat cctataaaaa ttctctctct gaggagaaag aggcctcaac
     2521 tacaggtgta aactattatc aattgaaaac aacagttttt acactcaatt agacttttta
     2581 ggggcaggat cactttcgct tgactgtagt ttgtcatttt tttgataaag ttagcacttt
     2641 ttcagaaact gttttgaaag tctcaatgat atagtctact tcttcatcgc ttaatttagt
     2701 atgaagaggg agggtaattt cattctcaaa gaaggcatag gccttaggat agttcgtcat
     2761 atcaaatcca agattcttat aggctgtcaa gagaggaagc ggtttgtagt gaacattact
     2821 tgcaattcct gctttagcca attcttggat gatgaggttg cgttcttcta ggcttgctcc
     2881 ttctacacgg gtgatgtaga ggtggcgtga agattcgaca gtttcagtct tgtgtgccaa
     2941 aggatggatg cgagaagctg caaaaccact atcatagcgg tccacaatgt ccttacggcg
     3001 ttgcaacaaa cttggatagc ggtccaattg taccaaacca agtgaagcca tgatatcggt
     3061 catgttgcac ttataggctg gtgtaacgat atcgtattcc catgacccca gttgcatctt
     3121 ggcaagagca tccttagttt gcccgtgaag ggaaaggatt tggaattcct tgtacatctc
     3181 ttcgtcatca atcactggat tggctttcca agtcgcactt ccaccttctg ccgttgtaaa
     3241 gttcttaact gcatggaatg agaaggaagt aaagtcagcg atagaaccag aaggttgtcc
     3301 tttataaata gatcccaaag cgtgggcact atcagagaca atgacaatac ggttaaaggc
     3361 cttttgccac ttgcttgaag cggtaaagaa gtcacgtttt ttctccacga cttggaacaa
     3421 acggtcataa tcgcaaacaa tccctgcgag ctctactgga ataatcacct tagttttctc
     3481 agtgatagct tgctcaagca ggtcatagtc catctcaaac gtatctgctt ggatatccac
     3541 catgacaggg gttgctccca cgtgcgtaat gacactacat gaagccgtat aggtcatggc
     3601 tggaacgatg acttcatcac caggtcccac ttccaaaacg cgtaaaatca actccagagc
     3661 ggctgtcgca gagttgagac aaacagtctt aggtgtctgt gtgtaaagag acaagcggcg
     3721 ctccagttct tttgttttag gacctgttgt gatccaacca gaacgcaggg tatccactac
     3781 ttcagtaatt tctgcttctg tgatatcagg cggtgaaaat ggaatattgt aatttggcat
     3841 tgatttgctc cttttatctg tactcatttt cttatgacta ctttatttta gtacctcaaa
     3901 cacggtttga aacatgattt tgatgtcccc aaagaaacta aactctcgga gataggcgag
     3961 gttatagcgc atcttttcag gaagaacatg ctccacatag gcctgatcaa ctgacagacc
     4021 tttctccgtc atttgactga taattgtgtc ctcatccttg tagttgatgc tggctggaga
     4081 ggtaatccct gcttgcaaga gcaaggttgc catcatttca gggctatact gctctgtata
     4141 acgtggcact tcaggtcgtg taccgacaaa ggacatctca cctttaagga cattgaccaa
     4201 ctgaggcagt tcgtccaaac ggacacgtcg gatgaaattt ccaaccttgg taatgcggct
     4261 atcgttagca gaagtcacca gacttccttt tttatccgca tccgtcacca tggtacgaaa
     4321 cttccaaatc ttgaaccgac ggttgtactg ggtcacacgc tcttgcttgt aaatcactgg
     4381 ccctttgcta tccaacttga tccaaatgct caagatgaga aagatgggag aggtcagaac
     4441 cagtaagacc aaggccaaaa cccagtccaa gcaacgcttg aaaatcagcg aacccttcct
     4501 tttagagaca agctggtagt aagactcaac ctcgcttgat ttcatttcca caggcaagtc
     4561 ttcccatttc agcatgctgt tctcctttgt ttttattata ctatttgtca acatttttca
     4621 ttataccaca aaatggaaaa ggcggtgaaa gaaatctgta atttgaagga tttccttcta
     4681 gtactcaatg aaaatcaaag tacagctttg agattgcaga taaagctgac atggtttaaa
     4741 gagattttca aagagtatcg gccatggccc ctgcctgcaa actatacatc ttgtgatagg
     4801 ttactcccag agccaagagt tcctcatggg ttccactctc gataatgcgt cccttatcca
     4861 agacatagat gcagttggca tcttgaatag tagaaaggcg gtgagcgata gcaatagttg
     4921 ttcggccctg tctcatcttc gccagagaag cttgaaccaa gctttctgtt tcagagtcaa
     4981 tattggctgt cgcttcatcc aaaatcagga ttttaggctg gctggcgact gttctagcaa
     5041 aggcaagaag ctggcgttgc ccagtagaga agctcgaacc acgctcggaa acaggggagt
     5101 cgtacccctg aggaagttct tgaataaagg aatctgcatc cacaaaggct gccgcagcct
     5161 gaacctgctc atcactggtt tcttggtaca tggcgatatt ggacttaata gttccatgat
     5221 agaggaaggg ttcctgcaag accaaaccga tgttttttct cagctcttct tgactgaaat
     5281 ccctgatatc cacatcatcc aagagaactc tccctgactg gaattcataa aagcgcatga
     5341 ggacattgat aatagacgat ttccctgaac ctgtatgacc tacaaaggca atggtttcac
     5401 ccttattaac agagaaagaa atgtcatcca gaatcggatg tttaccgtca tatgagaaac
     5461 acacatgttc aaaacggata ttgccttctt ggactttggc ttgcccattt tcttgaagag
     5521 gttcataggt cctctcgtct atcagggcaa agacacgacc tgcagaaacc atagccgttt
     5581 gcagagttga aaagttttgc gtcacctcaa tcaaggggtc aaaaaggcgg ttgatgtact
     5641 ggataaaggc atacatggtc ccgaccgtta tcccgataga aaaaccacgg tagccaaagt
     5701 aggccatcaa gactgcatag cctagaagtt tcagcaaact catggcaggt ctcaaaaaga
     5761 gggcatccaa ggctacagaa cggttggcgt agaccaagtg ttcttggttg atttcatcaa
     5821 attctgcctg caggcgcttc tcttgattaa aggcctgaat aatcctgatt ccctcgatat
     5881 tctctgccag cttactattg atatctgaca agagacttct ggttttctcg atgattttca
     5941 ctgacttttt tcgatagaga ttgaccaaaa ggaaaatcaa aggaagaaag agcaagacta
     6001 aagccgtcaa acgaaaatcc agcaccaaca tggtataaag ggttgtcaga aagataaaaa
     6061 ctgctgagat aaagctggat aaaatcccag aaaacatatc actaatcgtc tcggtatcgt
     6121 tggtcaaacg agaaacgata gaacctgctg gcgtcttgtc aaagtaagac atgcccagtt
     6181 tctccatatt ggcaaaggca tcccgacgaa tatccctaac aatactgtaa gacacgcgcg
     6241 caaagagaag attgcctaca tactgaacta cagtttgtag gatgtagaga ccatagtaga
     6301 ccagcaaaac ggtaacggct agttggttaa gattgctgag atactggtcg ataaagtggg
     6361 aagccacgag gggtatgaca cttttaatga ccatcgtcgc taggagaaaa ctgagtgcca
     6421 aaaaggtcag gagtccataa ggcttgagat aagacatcaa gcgcttcaat acagcccatt
     6481 gttcttgttt attctgcatc ttcttctcct ttcatttcca actgctgaga ctggtaggtt
     6541 tgggcatacc agccatccat cgctagcaag tcttcgtgcg tgcctcgttc gataatttga
     6601 ccattttgta gaactaaaat aaaatctgca tggacaacag cactgaggcg atgggcagtg
     6661 ataatggttg tcttgtcctt tcgcgtctcc ttgaggttgt cgataatcgc atactctgtc
     6721 ttggcatcta cggcggataa ggaatcatcc aaaatcaaga tatcagggtc taaaatcata
     6781 gcccgactca tagccaaccg ttgcttttga ccaccagaaa gactgactcc tttttcacca
     6841 atcagcgtat caaatccttg aggcatgtct acaatatctt ggtaaactcg ggctagctta
     6901 gtagcttcct cgaccgctga aaggggcaag ttaggattgc caaagcggat attgtctagg
     6961 attgaagtcg caaagagaaa ctggtcctga ggaacatagc ccatgagact gcgaaggtct
     7021 gtcagacgat agtcccgaat atcgtgaccg tttagataaa tagtaccctt atccacatcg
     7081 tattcacgca agaggagctt gattaaggac gttttcccag agcctgtctg cccaaccaag
     7141 cccagtgttt gcccttttgc caaactaaag tgaatatccg tcagtgtttc ctcattttca
     7201 aaagcaaagc tgtcaatggc atactccaaa cgcccatttt caataccatc cagaggaaac
     7261 tcagggtctt gtacaggaga ttcctgagac aaaagatttt caatccgctg gtaagaaacc
     7321 ttccctcgct gagtagtatt aaagaggaaa ccgatggcca gaagaggcca gaccagcata
     7381 tccaaatagc tgataaaggt gactagattc ccaactgtaa tctgcccttc ctgaaccatc
     7441 aaggagccaa ccaaaagcgt taaaacatag gacgaaccaa caaacaagag aaccataggg
     7501 tcaaagagac tatcatattt catggtttgc aggttctttt ggaaggttaa ttcattgact
     7561 gcctgaaaag acttcaactc gtctgcctga taaccgaaag acttggtcac tttgatacct
     7621 gatacggact cctgtacctt gttattgagt tcagaaaaag cagcttggga ttcgccaaag
     7681 gccttatgag tctttctccc taggcgacta gtcgtatagg ccatgaaagg tagggggaga
     7741 atggcaacaa gagtcatctg ccatgagatg ctaaagagca tggtcaacaa agtcaccaga
     7801 gccgtgatag aggcatccac cgcagacatg acaccgccac ctgctaaacg agtcaaggca
     7861 ttgatatcat tggttgcgtg tgccatcaga tcacccgtcc gataggtttg ataaaaggct
     7921 gacgacattt ttgtgaaatg cttaaacaag cgagaccgca tgatctgtcc caagcaataa
     7981 gaggtcccaa ggatatacat acgccacaca tagcgcaaat agtacatacc aaaggctgca
     8041 agtagcaagt aaaataggct aagaaggagg tcctgctgag ttaattgccc cgatgtgatg
     8101 gcatcaatga ctcgccccat aaccatagga ggaatgagat tgaggacgga aaccaagacc
     8161 agggccacaa tcccgactag ataacggcgt ttttctaact tgaaaaacca ccagagtttt
     8221 tgaataatgg acataaaatc cctttctgat tgcaaatgga aacctgaggc caatactcaa
     8281 tgaaaatcaa agagcaaact aggaaactag ccgcaggctg ctcaaagcac tgctttgagg
     8341 ttgtagatag aactgacgaa gtcagtaacc tacatacggc aaggcgacgt tgacgcggtt
     8401 tgaagaaatt ttcgaagagt acaagacccc aggtttttct tattcataag ttacgactgt
     8461 aacagcaccc ttgtcatatt cagcaataaa gatattggct acattgtcat gcccttgttt
     8521 actgaggtta tcaagcaacc actcttcgct acgaccaatc gattccaaga catctacttg
     8581 aatcacaccg tctgtcacaa ctggatactt aggattttca tctcccattt gcacaacgat
     8641 gagttgccca ttttgctctt gcacagctcg tttgacttgc ttcatctgga aaatcccttg
     8701 gctacgaagt ttgagggcta catccgatgc agacaaacca actgaacgac aggcttatgg
     8761 gtcaatattc ccatttttga taaggagagt tggcttacca tcaatcaaac gtttcacaaa
     8821 gtgaacattg ttgttgagct atttgaaagt caagatcagt ttgattaaaa tttcgatata
     8881 attaagtgtc atttgttcac atccaccagt tcaattgcgt ctgtcttata caattcgact
     8941 ttctctaaca ggtacttgtc tggctcactt ccgtttaggg cacgataata gcgatctccc
     9001 accttgataa gtgcgccatc tgtgtttgtg gaagtattaa catagacttc tgacttgtct
     9061 actcccaaat ctttggaaac aacctcgatg aaatgaagtg aagtttgaaa tttattgtta
     9121 gaaacttgat tgttttgata agtcgtgatg ctgaccaaaa gcagagctaa ataaggccag
     9181 agctgaaatc atgaccaact cacgaaattt ggttcctttt ttatcatggt atgccttgaa
     9241 agcaaaaaaa aactgtcaca gctaatagaa caatcccaaa gccaaccatg ataccatttt
     9301 gctgaccgat ttggctgagt acatagtcat aagagtagaa tttcatgtat tttcactccc
     9361 tttcataaaa tcattcttaa ttataaacga aagagagtaa tttttcaaac catacgttgg
     9421 ggaaatagat ctttttttgg tataataaaa tttataatct gaatgaaaaa ggtaacttta
     9481 tgaaacttat ctcatggaat attgattccc taaacgctgc cctaactagt gactcagctc
     9541 gtgccaaatt gtcccaagaa gtcctacaaa ccttggtcgc tgaaaatgct gatatcattg
     9601 ctatccaaga aaccaagctt tctgccaaag gacctacaaa gaaacacgtg gaaattttag
     9661 aagaactctt cccaggctac gaaaacacgt ggcgctcttc ccaagagcct gcccgtaaag
     9721 gctatgctgg aaccatgttc ctttataaga aagaacttac acccactgtc actttcccag
     9781 aaatcggtgc cccttctacc atggacttgg aaggccgcat catcactcta gaatttgatg
     9841 catttttcgt aacccaagtt tacactccaa acgctggtga cggtctcaaa cgcttggaag
     9901 aacgccaagt ctgggatgtt aaatatgctg agtatttggc tgaactagac aaagaaaaac
     9961 cagtccttgc gaccggtgac tacaacgtag cccacaatga aatcgacctt gcaaatcctg
    10021 ctagcaaccg ccgttcacct ggatttactg acgaagaacg tgctggattt accaaccttt
    10081 tggcaactgg atttacagac accttccgcc atgttcatgg cgatgttcct gaacgctaca
    10141 cttggtgggc acaacgcagc aaaacttcta aaatcaacaa tacaggctgg agaatcgact
    10201 actggctcac aagtaaccgt atcgctgaca aggtcactaa atctgatatg attgactcag
    10261 gtgctcgcca agaccataca ccgattgtct tggaaattga cttgtaagga ttttctatat
    10321 ggactacaat gcggtcattc ccgagttgct tgtatcgaat atcgaacagt cccggtcctt
    10381 ctactgtggt ttactgggtt ttaggattga ataccaacgt ccagaagaaa actttctctt
    10441 gaagagtgtc aactaatgtt agaggaggga acaaaggatc aactagctga actgacctac
    10501 ccctttggtc gtggagtcaa tctctccttt ggcataaagg atgttccaaa actctattaa
    10561 agagtgatga aggcaaacta tcctatttat cgtcccttga ccaaaagaaa gtttcgtgtc
    10621 agtgatccct atatctatcc tcataaattt gcagttttgg atccagatgg ctatttttta
    10681 agatttagcg agtagaataa taaaaattgg gaaaagtctt taaagttaga aaaaggcata
    10741 ttgtcaggtg tctaaaaact tgatagtatg ccttttatga tggaaaaatc ttctaatact
    10801 cttcgaaaat ctcttcaagc cacgtcaacg tcgccttacc gtaggtatat gatactgact
    10861 ctgtcaattc tatccacaac ctcaaaacag tgttttgagg ttgtggatag cttcctagtt
    10921 tgctctttga ttttcattta gtataaatgt tagcttacaa tggagttttt gttcaccccc
    10981 tcttctgttt gttcgttttt taaatcaaaa cactaacaaa atagttgatt tataaagtta
    11041 tttagtgtaa aataaaaaca ttggctcaag cctatttaaa attgaatatc gttttacttg
    11101 tttatgtcgt gaattggcac gacgtttcta caaggtgccg gaacacctaa caataagtaa
    11161 gtcagcagtg agatatggtc gtttttgcca tgcctatttg ggggacttac ttagagatta
    11221 agtaggtcct ttttgaatat ttttaatatt tcccttaatt agtgcggacg ggaggtgaaa
    11281 ttatcctgtg aataatttca ggaattcagg gaatttcatc cctaattttt gagcccctag
    11341 ctctttcatt tctaggctta ggctaaaata gttccccgaa ctattttact ctcaaaaatc
    11401 ccgtcataga caataacaag attattgtct atgataccac tacatcggga aatatttgat
    11461 aagactatta cgagcgaagc gagtcttatc aaatatatag tattagcaaa agggatcact
    11521 atagaaacat ttcaacaaca agtaaattag cttttagact ttaggaggtc ttatgaaatt
    11581 attagaagat cgcatcctca aggatgggca tatcttgggt gataacatcc tcaaggtaga
    11641 ttccttttta acccaccaag ttgactttag cttgatgcga gagattggta aggtttttgc
    11701 ggaaaaattt gctgctgccg gcattaccaa ggtcgtaacc attgaagcgt cgggtattgc
    11761 cccagccgtt tttacagctg aagccttaaa cgttcccatg attttcgcca aaaaagctaa
    11821 gaacatcacc atgaacgaag gcatcttaac tgctcaagtc tactccttta ccaagcaggt
    11881 gaccagcact gtttctatcg ctggaaaatt cctctcacca gaggacaagg ttttgattat
    11941 cgacgatttc cttgctaatg gccaagctgc taaaggcttg attcaaatca tcgaacaggc
    12001 cggtgccaca gtccaagcta tcggtatcgt gattgagaaa tccttccaag atggtcgtga
    12061 tttgcttgaa aaagcaggct accctgtcct atcacttgct cgcttggatc gttttgaaaa
    12121 tggtcaggtc gtatttaagg aggcagatct ctaatgcaaa ctcaagaaaa acactcgcaa
    12181 gcagccgttc tgggcttgca gcacttacta gccatgtact caggatctat cctggttccc
    12241 atcatgattg cgacagccct tggctattca gctgagcagt tgacctacct gatttctaca
    12301 gatatcttca tgtgtggggt ggcaaccttc ctccaactcc aactcaacaa atactttggg
    12361 attggactcc cagtcgttct tggagttgca ttccagtcgg tcgctccctt gattatgatt
    12421 gggcaaagcc atggtagtgg cgctatgttt ggtgccctta tcgcatctgg gatttacgtg
    12481 gttcttgttt caggcatctt ctcaaaagta gccaatctct tcccatctat cgtaacagga
    12541 tctgttatta ccacgattgg tttaaccttg atccctgtcg ctattggaaa tatgggaaat
    12601 aacgttccag agccaactgg tcaaagtctc ttgcttgcag ctattactgt tctgattatc
    12661 ctcttgatca acatctttac caaaggattt atcaagtcta tctctatttt gattggtctg
    12721 gttgttggaa ctgccattgc tgctactatg ggcttggtgg acttctctcc tgttgcggta
    12781 gctccacttg tccatgtccc aactccactc tactttggga tgccaacctt tgaaatctca
    12841 tctattgtca tgatgtgtat tatcgcaacg gtgtctatgg ttgagtcaac tggtgtttat
    12901 ctagccttgt ctgatatcac aaaagatcca ctcgacagca cgcgccttcg caacggttac
    12961 cgcgcagaag gtttggccgt acttctcgga ggaatcttta acaccttccc ttacaccaga
    13021 ttttcacaaa acgttggttt ggttaaattg tcaggcatca aaaaacgcct gccaatctac
    13081 tacgcagctg gtttcctggt tctccttgga ctgcttccta agtttggtgc ccttgcccaa
    13141 atcattccaa gctccgtcct cggcggtgcc atgctggtga tgtttggttt tgtatctatt
    13201 caagggatgc aaatcctcgc ccgagttgac tttgctaaca atgaacacaa cttccttatc
    13261 gcagctgttt caatcgctgc aggtgtcggt ctcaacaaca gtaatctctt tgtcagcatg
    13321 ccgacagcct tccaaatgtt cttctcaaac ggaatcgtcg tagccagcct actcgccatt
    13381 gtcctcaatg ccgtattaaa tcataaaaag aaataagaaa aagaggtgcg agcctctttt
    13441 ttgtgtgaca aaataaaaac atctacctat tcatatatgc tagtgtcata gtcctgaaaa
    13501 tcatctgcat caagaacaat ttcacaactc ttatactttt ctcttacaag tcgttcggct
    13561 tcatctggat tttcagcctc tatacttact aaacgtgata aagtttctgt aatttctact
    13621 gtatactttt tcagttttag attgctttcc aataattcac ctattaacaa taatctatga
    13681 taggagctat gtagccactc tggcaattta tctcctactt cccacccata tttttccttc
    13741 atcaaagcta gttgtttttc gtcaccaaat ttcatatttt cagataacca tctcacacaa
    13801 tcgggatctg ataaggtaca gacgattaaa cttaggttat cgtaacggct ttcagtttca
    13861 taatcaggcc agatgacttt taagaaatca tacacctctt ctgatgcaac aagttgtttc
    13921 gttttcataa tttccgatac tttcctctac ctttaaattc tattatttct ttgtctctta
    13981 atatttgaag ctgttgccta atcttttctt tgatatgatt gttcttaaca aagatatttt
    14041 ttaggtcact ttcaaaacga tacatatctt caagggtaaa ttctgaaccc tctatcttat
    14101 ctatacaatt tagaatttct attgtccaac ctcttgatga cagagagctc ttccttaaaa
    14161 ataaaccttg cttaaattct tttgtaactt tttctggatc tctaacttgt ccatcttgca
    14221 caagaaatat ccttccttta gaaggtactt gtgataaatc aatgttacaa ccaatccaac
    14281 ctgctcgtct agcagttggt gcaagtggtt ttctttgaat aatcgatttc ggtgtaacaa
    14341 attgcttcgg aaggacaaga aagttattta cctcaaaatt ttttgtgtaa gttaaaaaaa
    14401 agaaattagg attattatct gcctgcacac gcttcatcat cgttgcataa gcaccatcat
    14461 tgattgttga tgaaaaattt ccttttttgc tctttagttc aaactcctca ctacaatgat
    14521 tacagtaaaa atctgctaca ggccgattat tttcaaaatg atttaaggga ttgttcccac
    14581 aattagggca ataagactga cgataaaccc aatcctccgt taagatacgt gccttttggc
    14641 tattactttt ataggtttct actaattcta aattaaagtg taattccatt taaaaattcc
    14701 tcactaatat gagaactcac tcgatataag caattaaaat ctactataac tagtatacca
    14761 catatatgta cctattacat ttgtatttca tcactctctt ttatatttcc ttctaacata
    14821 tctcataaat taaaaatgag tgagataaat ttatcccact cacctttctt tatatactta
    14881 cccgtctttc ctcagacctt tggcctgtga caaacatggt aaaggttgct ttacagacat
    14941 ttctgccctc ttgattggtg atatccacat ccatcacaca ggttgtacga ccttgatgga
    15001 cacattctcc tttaatggtc aatacatcgt cgagttttcc tgccttaagg tagttgatag
    15061 aggattggag tgtcacccca tcaagtccca gcgagataac caccaaacca ctgatttggt
    15121 cacatagcgt gaatagatag ccaccatggg cattgccata gtagttgagc gacgagttca
    15181 ctactttggt cgtcaccaca acgtgaccat ctctcatttt ttcaatttcg taattttcaa
    15241 aggcagatat agcgtcaaaa tgaaagtctt tcatcgtttc ctcctgatat ttcaaacgac
    15301 actatgatac tactttttat aggactgtgc aaggagaaag ctcctagtct agtaaaattc
    15361 cgacatgttc cacaaagcgc ataaaggctg tctgcccttg ttctgtctgc ttgtagactc
    15421 ctgcatcctc aagtacacgc gcaaagatag cacccacaga gtccttgacg attgcaaggg
    15481 ctttttcttt atccgttagg tctggatgtt gggatttgag ttggtctgcc cactcctgat
    15541 gataatcggc aactgtaaca gcttctccta caagatagct cgcgacttgc tccacttctt
    15601 ctttcagacg tggtggcaag attgccaagc ccatgacctc aatcaagccg atattttcct
    15661 tcttgatatg ttggacatcc ttgtggggat gatagatgcc atcaggatgc tctgctgaag
    15721 tctgattgtc tcgcaagacc aagtccaact caaactgtcc atcacgctta cgcgctattg
    15781 gtgtgatggt gtgatgcggt gtcccgtctg tctctgctaa aatctgcaca ctagaatctg
    15841 aatactgacg ccattcctgg aaaatcttat cagctaagtt aatcaaatcc tctttggaat
    15901 ccgaagtcaa gcgtaggaca gacattggcc acttgataat tccagccttg acctgctcaa
    15961 aaccagcaaa tcggaaggtc ttttgcaagg gagccaattc cataggaaat acgtgacggc
    16021 ctccctgata atgatcgtga gttagaatag agccccccac aatcggcagg tcggcattag
    16081 atccagcaaa atatcctgga aactgctcta cgatagccaa cagacgttca aaactctgac
    16141 gactaatggc catgggacga tgctggccat ctaaaaagat acaatgctca ttaaagtaag
    16201 catagggcga atactggaac ccccattcct gaccaaccat ttcaaaacgg ataatccggt
    16261 ggttgctgcg agctgggtgg ttgacccgac catggtaacc ctcattctct aaacaaagtt
    16321 gacactgagg ataattacta gcttgcacca acttggctac cgcaatctct ttcggatcct
    16381 tttcaggctt agagagattg atggtaattt caagttctcc ataatcagat ggaacacgat
    16441 aagcgatatt tttagcaatg gccttgagtt tgatgtaatc atttttctga ctgagttgat
    16501 aaaaatcctc tatcgcttgc tcaggagagt ggacataggc ttcccagaag tcacgattga
    16561 cctgactcgg ataaggagtc accaagtcca tcagttcagt accaaggatt tcacgcgcag
    16621 tctgactatc ctcaatcatc tctaatcgaa cggcttcctc aactagctgg tccttgaggt
    16681 caatcaattt atccagattg gtctcaactt ccaaaacacc ttctcccact cgtgccaaaa
    16741 cacggttggt caggtagatt cgatccattt cctcaaatga actttcagaa atgacatgtg
    16801 ttacaaattt atctactaag atcactatag agcctccttt tgactagtca aggacgcgag
    16861 tgccacctgc aacttcagcg atatagaagc ttggagcgta tccaactact tcctcgtagt
    16921 gtttgcctac agcttcctta aaggcctcaa cagtatcttt ttgaaccaag gcaatggcac
    16981 agccaccaaa accagcccct gtcatacgag caccgagaac tccttcttgt gcccaagctg
    17041 tgtgaacaag agtatccaat tccaaaccag ttacttcata atcatgctcc agagaaacgt
    17101 gtgacgcatt catcaaacga ccaaatgttt ccaaatcacc tgcttgaagg gctgcttgag
    17161 ctttaagggt acgttggttt tcaagcacag catggcgagc acgtttcaaa cgattttcat
    17221 ctttaatcaa atagctatat tggtcaacgg cccactcgtc caattcaccc agggtctgaa
    17281 tatccaaggc aacttgcaat tcttccactg ctttttcaca ctcagcacga cgttcattgt
    17341 atttagagtc cgccaattca cggcgtttgt tggtgttcat gataacaacg acattgtcct
    17401 tcaaatcaag tggcaccaag tcgtattcta aagtattggt atctaagtaa atagcacgtt
    17461 ggtcagcccc cataccaata gcaaactggt ccatgatacc agagttgact ccgataaagt
    17521 tgttttctgt ttgttttccg attttaacca aatccaaacg gtctagtttt aaatcaaaga
    17581 gatgctctgc cacgactcct gtcaagagct ccaaggatgc tgaagaagac aaaccagcac
    17641 catttgggat attcccataa acataaaaat caaaaccttt gtcaatcacg tgcccagctt
    17701 cttgcaagaa atgaagaacc ccttttggat aattggtcca gttgtgctct ttttcaaact
    17761 tgaggtcagc gagaggcact tcgataatgc ccttgtcctc aaagttagct gagtagaaac
    17821 gcaagacttg gtcgtcacgc ttacgagctg caccgtaagt tcccaaggaa atagcagcag
    17881 gaaaaacgtg cccaccgttg tagtctgtgt gttcaccaat caaattaata cggcctggtg
    17941 aaaagaaggt ttggtctgct tcttgaccaa aaacagcaag aaagtctttg cgaaggactt
    18001 cagtagtaag atgttgtgcc atatgaattc tcctttgact gtccgttaag ccatcttaac
    18061 gtgtaatcgt tttctttcta ttgtatccaa ggaatttccc aaggtcaatc ctttttacta
    18121 aaattttact aaaatatcag taaaaagaag aaaaatatga tatactaacc taatcaagga
    18181 ggatttatgg ctaccttaaa agacattgca cagctagcct ctgtctcaat cgcgaccgta
    18241 tcccgtgtcc tcaaccgcga ccagagccta tctgttacag aagaaaccag acaccgtatt
    18301 ttaaccgttg ctgaagagct aggctacacc aaacacctca agacaggcga gtcccacaag
    18361 cccaagcaaa agattgccat tatccaatgg gtcagcgaac aaggggagct agacgacctc
    18421 tactactacc agattcgcct aggaatagaa aaaagagccc aagagttgga ctacgatatc
    18481 ttgcgctatt ttaatgacca tccttttacc ttaagcgagg aagtgattgg gattctctgc
    18541 atcggaaaat ttagccgagc tcagatttct gcctttgaag aataccaaaa gcctcttgta
    18601 tttctagaca gcgatacact ttccctggga catacctgta ttatcacgga tttttacact
    18661 gctatgaaac aggttgtcga ttatttcctc agtcaaggga tggaccgcat cgggattcta
    18721 acagggcttg aggaaacaac agaccaagaa gaaatcattc aggacaagcg tctagaaaac
    18781 ttcaaaaact acagtcaagc gaggggaatc tatcatgatg aactggtctt tcaaggaaga
    18841 tttactgccc agtctggcta tgacttaatg aaggaggcca ttcagagctt gggagaccaa
    18901 cttccgccag catttttcgc agccagcgat agtttagcta tcggtgctct ccgtgccctc
    18961 caagaagctg gaatcagcct gccagatcgc gtcagcctca tttcattcaa cgacaccagt
    19021 ctgaccaaac aggtctatcc tcccctctct agtattacag tttatactga agaaatgggg
    19081 cgagcaggta tggatattct taacaaggaa gtcctccacg gtcgcaaaat tcctagcctg
    19141 accatgctgg gaaccagact gacattaaga gaaagtaccc taaatcaaga ataggataac
    19201 ataaaaaacg aatagagttc taaaactcct attcgttttt tattcgatta caatcataga
    19261 cttaatggtc ttacgttcat ccatatcttt gtaggcttgg tcgatatctt ccagtttata
    19321 acttgaagta aagacgcgac ctggattgat atcaccatca aggacggctt ttagtaaaaa
    19381 ttgcttatcg tatgttgtag cagaagctgc cccacctgct acagagatat tttgcataaa
    19441 tgtcgaacca agagcacgat tattatagtg tgggactcct acaaagccca tacgccctcc
    19501 attatgaaga acacctagcg cctgttctat agcagcctcc gtaccaacac attcaagtgc
    19561 tgcgtctgct cctccaccga ggatttcacg caccttggta attccttctt gaccacgttc
    19621 tgcaacaaca gctgtcgcac ctgactccat agccatcttt tgacggtctt catgacggct
    19681 cataaggata atttgtgatg ctccacgcat cttagccgcg atgacagcac attgaccaac
    19741 agccccatca ccgataacaa caaccttgtc ccctttttga acatttgcaa cacgcgccgc
    19801 atgatagcct gtcggcatga catctgcaag agtcaaaagg gacttgagca tcccttctgt
    19861 atagtcagaa ggttgaccag ggattttaac cagcgcccag tttgcatagt ggaagcgaat
    19921 atattctgcc tgaaaatcac cccccaaatt attgccaata tgattgtcgc aagaaccgtc
    19981 aaatccagca agacaggcat cacactcacc acatccatgt gtaaaaggga caatcacaaa
    20041 atcacctggt ttcaccgtcg taatggcttc cccagcttct tcaacaatcc caatcgcttc
    20101 gtgtccacta tttttgtgtc cagctttcgt ttctggatta cggtacctcc ataaatctga
    20161 accacaaacg cacgcacgaa ccacacgaat aatcacatca tccgcttcta ttatttgcgg
    20221 acgttcaatg ctagcaagtc caacctgacc tgcctttgta tatactgctg atttcattta
    20281 aaattttcct tccttataaa gtttaatttt gagatttaaa cgatttaaag ctgtctgtag
    20341 ctgagacaag cgctcctcta atttttgctt ttcctcttct aaaataccaa gcctctcctc
    20401 tctcgtttca tctccctttt ggtagagcga catatagtca actaaactat ctacagagac
    20461 acccgccgaa cgaaaacact taataaattc cagcgcttcg atatcctgat cttgaaaatc
    20521 acgaatccca gtagcagtac gagtaatcgg tggcacaaga ccaacccgtt cataataccg
    20581 aatcgtatcc gctgaaattc ccaacaaatc actggcagat ttaatattca cacgctaacc
    20641 tcctctcatt tcattcactg aattcacgct attctaactc acttatctta ttatataact
    20701 ttgagttgac tccaagtcaa gcattttgta tctaatcgaa aaatttttat cattaacaat
    20761 catatatctg gagtttatac aatgctcttc tcttaccaat ttttgaaata tttaaataat
    20821 aaagaaatta ctctattcca aataagatag tacccaagta tttttcactt ttttctaatg
    20881 ttgatgctca taactccgtt ccaagtcaca cacctttcgc ttatgggttt ggtgagtttc
    20941 taggtcagca tcaatttcaa tggtaatatt ttgaaaacca caatctttta ggaaaattcg
    21001 aatagactct ttacaagttt ccatatgttc catttctttt agacaaacat ggacaatggc
    21061 atttttttcc aaagcatcca tagtccagag attaagctga ttaaggctgg ccacattgtc
    21121 caatcgctcc aggccactct ttacttgctt gatatcaaga ccttctggca cagcatccaa
    21181 gaaaatcttg agtgtagacc aaaaacgtgg aagggctttt gaaagaataa agaaagaaat
    21241 gacaagggac aaaagaggat ctaggatata ccagtccgta aatcgaagaa caatcgccat
    21301 caggataaca gctacccacc ctagcgtatc ttccagaaaa tgcagactca gaatagactc
    21361 attctttgtc tttcccttac caaccaccag actcgctaac agattgatag taatcgcaat
    21421 aattcctaac cagagaatcc cctcatcatt gactggttgc ggatgcaaaa tcttcgtgac
    21481 attttccaaa atgactagaa cagagcccgt tacgagaatc acagctgtta ccaaggctcc
    21541 tagcaggcta aaccgcttat agcccaaggt gtactgattg tcttcttcac gattggagat
    21601 tgtttctaga aaagctgata ttccaattgc aatcgcatct cccaagtcat gcacagagtc
    21661 agcaagaaca gcgctagaac caaatactcc acctgcaata aactcaacaa tggcataagt
    21721 caaatttaag aaaaaagcca cccaaacagc atattttgcc ttcatatttc tcattccttt
    21781 gttataatag atttatgaac accttgttca ttatcattat ccactaaaac tcaaagaaag
    21841 gatagaagca aaacgtcagc tttattcagt tctgaacaat ttgcctcaag tgtccatatg
    21901 actaacattg accgccgtat cagcaaaacc aaaaaagcca tctatcaagc ttttatacaa
    21961 cttttgaatg ctaagggcta cgaggccact actgttcagg atatcattga tctcgcagat
    22021 gtgggacgat ccacctttta ctgtcactat gagagtaagg agcttcttct ggaccagctc
    22081 tgccgctacc tcttccatca tctttttgaa agagagcaag ccatttcaac cgaggactac
    22141 ctcgcccacc tctttctgca ttttcataaa aaccaagacc atatcaccag tctgctattt
    22201 tccaaaaatg actacttcct ccgtcaactc cataaagagc tagaacacca tgtctattcc
    22261 gtgctagctg ataatttaaa agaagcccac cctaatctgc ctacttctta cctccaacac
    22321 ttggtcatgt ccaactttat cgagacattg acctggtggc tcaaaaaagg tcaagatttc
    22381 acagaccagg aagttgtcca attttatcta gaccttctca ttcctaaaaa ttgaatatag
    22441 agtaaagctc agttgtctta tttctagatt actgagtttt ttatcttttc aacaacaaaa
    22501 gaggacccgc cgatcctctt tttcatacta taaatccttg attatcaact atatctgttt
    22561 taatcgaaat ctcaaaacag cactttcaaa catcttttcc tagttaagta aatcagtatt
    22621 ctgcttagct gccttgctcc attgatacca accaactaga ctgttaatga gataaattag
    22681 atatttccct tgaatttgca ggctttctcc ccaccagaga tagattgaaa agacattggt
    22741 agccgcccag aatatccact gttcacggta aacagctgtc atgaggattt gccctacccc
    22801 attggttgca tctgtgattg aatcacgata gggacgattg gcaccaatag actgataaat
    22861 gaagccaaag gccaaccacc aaagcacact aatggaaaga tactttgtcc agcccttgcc
    22921 gtccagttta cgcgcgacaa actcctgctt ttccttctta aactgtgcct gataaatcca
    22981 aactagaagt ccaattggct gcatgactgt gaagtaaagt gtcgtcagca cctcaccata
    23041 aaagcctttc tgtagggcca aaataaggta aataacagag ttaatcaagc caaaaagata
    23101 attacttgct cgaccttccg atacaaagat tacacagata atccctgtca agctacaaat
    23161 catcccaatc cagtcaacaa tacgatgttc gtaaaccaac tccagccaga gaggaaaact
    23221 tcctaaaacc agcaaataaa tccactgggc aaaactacga tgggcaaaga ggtcatccca
    23281 gatagccttc atagttcctg aaaatcctaa atcagccata gccgcaacca tacgacggta
    23341 accacctgac atttcaccta gggttgtttt gatattttca attttctttt gcaaataagt
    23401 atgcatcatt tctccttttg tttttaaaga gccgtgtctg gatagacttt cggacgcaac
    23461 gctctattag ataatgaact gcctatacac aagatttcta accttagtcg acatgagctg
    23521 aaacctctta tttgttaagt agttcacaaa atattataca cctattttat gaatagtcaa
    23581 ctgtctttac agtaaaattt tagaaaatca tgaaaatttt ctctttcttt ccattttaag
    23641 tgacattcag tcattctcac atcaaaaaag cccagacgaa attgtctgag cattctttta
    23701 tctagtcgtt taaggaagtt gagttcagta tgtttaaagt ctctgtccca tcatttcttc
    23761 aacaaacctt gttcttggag aaactccttg gctacttgct ctgctgactt gccttcaaca
    23821 ccgacttggt agttgagctg gctcatctgg ttttctgtaa tcttaccagc caatgtatta
    23881 agaactcttt ccaactctgg atgtttcttg agaagagctt ctttcatgag tggagcccct
    23941 tgataaggtg ggaagagttg cttgtcatct tccaagacct gtaaatcata acgctccaat
    24001 tccgcatcag tcgaataggc atccgtgatt tgaatatccc ctgactgaat tgcctgataa
    24061 cgaagagctg gctgcatagt cgccacattg agattgagac catacattga ttgcaagccc
    24121 ttatttccat cttcacggtc gttaaactca agcgtaaagc ctgccttcag ctgcccttcc
    24181 acttttttca aatccgaaat ggtcttcaag ccatattctt gagcaatctt tttcggaaca
    24241 gctacagcat aggtattttg ataagacata ggtttgagat aggctagatg atcctgcttg
    24301 gcaatgccat cacgcgccac ttgataaacc tgctctggct catgactcac cttgggtgat
    24361 ggttgaagca aactttcagt caccgtaccg gtaaattcag gataaatgtc aatatcacct
    24421 tttttcagag cttcataaag gaaacttgtc gtcccaaaat tcggtttaac agtcgcagtc
    24481 atgctggtat tttcttcaat cagcaattta tacatattag ccaaaatttc tggttctggg
    24541 cctattttcc cagcaataat caagttttcc ttctcttttt gagccaaaag ggctggacta
    24601 taagacagac ccagtaataa agccaccaag gcaaaaccag agaaaatcgt ccgcaatttc
    24661 gctttttcca ttacttttag taggaagtta aaggcaatag ctagcactgc agaagaaagt
    24721 gccccaatca aaatcagact ggcattatta cggtcaattc ccaaaaggat aaaggaacct
    24781 agtccccctg ccccaatcaa ggtcgccaag gtagccgtac cgataatcaa gaccgctgcc
    24841 gtccgaattc cagacatgat aacaggcatg gcaagtggaa tttcaaactt cttgagacgc
    24901 tcccatctgg tcatcccaaa ggcaatccca gcctcttgca gactcggatc aattccattc
    24961 agcccagtaa tagtattttg taaaatcgga aaaatcgcat aaatcactag agctgtcaat
    25021 gccggtaaag tcccaattcc catcaaaggg ataaagagcc ccaacaaggc cagagacggg
    25081 atggtctgga aaatacctgc aatctgcaag acccagtcgg ccagcttctc atgatagcga
    25141 agataaacag ccaagggaat cgcaagcaaa atagctagta acaaggtcaa aagtgacaac
    25201 tgcaaatgtt gagatagggc tgtcaaccaa tcaccaaaac gatcctgaaa agttgcaatt
    25261 aaattagtca tgaatactac ctccaaacaa gtctgcaaca aagtctgttg caggagcttt
    25321 taaaatagtc tcaggattcg acacctgacg aatctctcca tcctgcaaga cagcaatacg
    25381 atctgccaac tttaaggctt catccgtatc atgagtcaca aaaatcgttg tcatcccaaa
    25441 ctctttatgt aattcttttg tcagaacctg caactgtttt ctcgaaatag catccaaggc
    25501 tgaaaagggt tcatccatga ggagaatctt gggctgacca atcatagctc ggacaatacc
    25561 gacccgttgc tgttctccac cagatagctc acttggaagt cgatgcccat actcagctac
    25621 tggtaaacca accttagcca aaagctcttc tgttttttga gcaatttctt ccttagtcca
    25681 acccttcatt tcaggaatga gagcaatatt ttcctcaact gttagattgg gaaagagagc
    25741 aatagcctgt aaaacatatc cagtagaaag acgaagttca cgctcatcat agtctttgat
    25801 gcgcttccca tccatataaa tatttccatc agttggttcc aaaagacggt taatcatctt
    25861 gagcatggtc gtcttacctg acccagaagg ccctactaaa accataaatt ccccatcctc
    25921 aatctgtaag ttgacatctc tcaagacatc cttttctgtg tagcgcagtg ctacattttt
    25981 gtattcaatc attctttgtc ctcaatttaa aacttccctc gattagtcaa gtcttctacc
    26041 ttaggcatga cttccttatt atcccaatgc tccacaattt tcccgttctc taaacggaag
    26101 atatcgtact gggcataagc aacgccatca atctgagtct gaccatagct aaccacatag
    26161 tttccttgtc ctaagagttg gaaaacaaag tcaaaagtga cactatattc agccacatag
    26221 tttttataag cagcacttcc ttgtccaatc tcatgattat gctgaatcaa atcgtctgcc
    26281 acataatcac tccactgctc tagctcccca ttttggaaaa tttctgtcaa gaaacggcga
    26341 accagctttt tattttctgc tttcttatcc aaatccttga tttcaaaatc tccaaaaatt
    26401 tgatctagtt ggtcattttc aggtgttcga tagtagtcaa tgacatccca atgctcaaca
    26461 atacgaccat tctcatccgc acgaaaagta tccgtcgtca cccattgagc ttctccacca
    26521 ttcagatatt gatgaacatg aacaaagacc agattgccat cctcaatggt gcggacaatc
    26581 ttaatctgac gctctggatg acgctcaaag aaatctgcaa agaaggctgc aaatccttct
    26641 ttcccgtcag gaacacctgt cgaatgttgg atataggtat cccctacaga ctgggcttga
    26701 gcctcagcaa ctcgtccgtc ttgaatggca tggatgtata ggttgtgagc atttttcact
    26761 tgttgtgaca tattctaaac ctcatttccc ttctctttca aattcgccaa aattctttct
    26821 tgaaaacctt caaattggtg aatttcttcc tctgaaaatc ctttgtaaaa gatagtatcc
    26881 aatttctgac tgacacgatg ccccacttct ttctgggact tgcctaactc cgttaaaact
    26941 aaatacttct tacgcttgtc ttttccacac ggactaacaa ttacaagctt ttgttcctct
    27001 agctttttta tcatagtcgt cagcgtatta ttcgcaagcc cagtcgcaag cgcgatatct
    27061 gtcgcagttg cgcagccagt ttcactattc cataaaaccg ctaaaatctt gccctgttca
    27121 cccctataaa gagcctcagg atcttgactc agtaactttt gaaaaatccg cccattcaac
    27181 aaacgaatat gatgggctag caaatgacca tctttcataa cacctccaat ttatttcgat
    27241 atcgaaatga ataaaacaat tgtaacactc atcgttctaa ctgtcaacta tttcgattta
    27301 gaaataattt ttgataatta tccacaccac catactccgg ctcaactaac ttttaacgag
    27361 agtttctaaa ctccttcgtc ctccagtcta caaaagcctt ccattcgtac tatcctatat
    27421 tttatgaggg gacacatttt tcctatcaga ccatttattt taaagataga agtaaatcat
    27481 aattgcttcc atctgttctt ttatagtata ttgaagttag actagagcac tgtatcttct
    27541 aaaacattga tagaaagcga tttgaatttc ccaatcaatt tgttcgtatt tatagcattt
    27601 cgaaactgga ataggacacc atgactgcta aaagatttct ataaattcat ttgattttcc
    27661 taatcaattt gttcatatct tatttcattc cgctataatt tcaccttacc ctatcttttt
    27721 cgtagcaccc ttcaaacagc ctatccccta ccgtttgacg attcctcact tcgctccact
    27781 tccattacag aagtttcttc actactatgg gctcggctga cttctcatga ttccttgtta
    27841 ctactatttg aacgctcacg agatagatct tacaaaaaat gctttgatcc acaatggaat
    27901 caaagcattt taaagagttc ctcatacata agcgcagaag tcgcagttcc tctgtacttg
    27961 gcttcttctc ttttgataaa gcgagccaag ttgagcaact caggtgctgg atgtttggga
    28021 tttaggagca attcacgatt gaccaggcct gagagacgaa ctgcctgcaa ttgttcattt
    28081 gtagtaggca gttttttagc agtctctagg agagcagcaa ctaaatcttc actcaaatca
    28141 tgtcgagcat gattgtaaag atcttttata aggctttcta ggtttggttc tgccatccct
    28201 accacctccc ttatggttta ataatgttta atcaaatcaa ccgttgaacg atccaatttc
    28261 ttcaccaagg cttgtaagaa agcttgcgct tctaggaagt catccattgc atagagggtt
    28321 tggtgagaat ggatataacg agcgcagaca ccgatagttg ttgatgggac accaccattt
    28381 ttcagatgag ctgcacctgc atctgttccg cctttaccac agtagtattg gtacttgata
    28441 ccagcttctt cagccgttgt caaaagaaaa tccttcatcc ctgggagaag caagtgacct
    28501 ggatcataga aacgaatcaa ggttccatct ccaattttgc cttgaccacc gtagacatca
    28561 cctgctggtg agcaatcaac tgcgaggaag acttctgggt caaacttggt tgtagaggta
    28621 tgagcgccac gcagaccaac ttcttcttgg acgttagaac ccagatagag ttcattgccg
    28681 agtttttgac ctgataaagc ttcagctagc tcgcttacca tgaggacacc gtagcggtta
    28741 tcccaagctt ttgagatgat atttttttca ttggctgtca aaattgcaga actatctggt
    28801 acgatggtat caccaggacg gatgccaaaa ctttctgcct cagccttgtc cgcaaaacca
    28861 ccatcaaaaa cgatatcggc aatggctggc atggttggtc ccccctttcc acgagtcaaa
    28921 tgcggaggaa cagaacctga aatcacagga atttcatgac catcacgagt caagagtttg
    28981 aaacgttggc tgctaaccac catggggttc cagccaccga tttctacgac acggaaggta
    29041 ccatctggct tgatttcgct gaccataaaa ccaacttcgt ccatatgaga agcgaccaag
    29101 acgcgcggtg catccacagc ttctgaatgt ttgataccaa aaataccacc caagccatct
    29161 gtcaccactt catccacata cggtgtcaac ttttcacgaa gataagcacg gacaggcgct
    29221 tcatgacctg agactgcagc aagttctgtt acttctttaa tttttgaaaa taatgttgtc
    29281 atttcagttc cttctttctt tcatccattt taccactttt tataggagaa ggatagtggg
    29341 aaggtggatt tctaagttag tatcttagtc ctgctctatc ttagaaaagg atagtattct
    29401 cttgcatgta gtgcaaaatc tagtaaacat tccaaaatta actcgaatat ttatttccaa
    29461 acaaaaaaac aatacaccat caaagttgtt tagatttttc atgaaattta cagaaaatag
    29521 ttgacttccc tttcttcttt ctttaaatat atagttggtt gagtttggaa tagtacgctg
    29581 tagctgctaa aacatttcta gaaattaatt tgactttcct aatagagttg ttcatatctt
    29641 atttcaattt actatagtac aaaactagaa aaggaaaaaa tcatgaccaa aacaattctt
    29701 gttacaggtg ggactagcta cattagctcc cataccgtta aagctctttt aaatgctggc
    29761 tatcaggtgc atgtcctcga taatctctcc acaggaaatc gagcggctgt ggatagtcgt
    29821 gctagcttta aagaactgga tgtttatgat gctagcgcct taaaggctta cttagaagaa
    29881 aatcagattg atgctgtcct ccattgtgca ggtgaaattg ttgtgagcga aagtattgaa
    29941 aatccaagta aatacttcac tgccaacgtt gctggtatga accaagttct caaagtctta
    30001 tctgaagttg gcattcaaaa aatcatgttc tcttcgactg cttctctcta tggaaataac
    30061 tgtattgaca agccggtgac tgaagatacc ctgctcgacc ctgtcaaccc ttatgcagag
    30121 acaaaactga tgggcgaacg aatgatttac tggatggcca atcgctacga ctggaaatat
    30181 gttattttcc gttactttaa tgctgctggg gctgaaatgg atgcttcaaa cggtctgcgt
    30241 gtgaaaaatc caactcacat cattccaaat atcaacaaga ccgcattggg acaaaatgat
    30301 agcttaaaaa tatttggaga tgactacgat acacgtgacg gttcatgtat tcgagattac
    30361 atttatgtct tggaccttgc ataggctcat gttaaaggaa tgaactacct atttcaagaa
    30421 gacagttctt ctcaaatctt taacttagga actaaaaaag gctatactat caaagaaatc
    30481 tttaaaactg ctgaagaatt actaaattaa aaaatcccac acgagattgt tgctcgacgt
    30541 gctagtgacc cagccagtgt cctagcagat gcttcaaaag ctaaacaata tcttgattgg
    30601 acggctagct actccctcga agatattatt ttatcagatt aaggtaaaaa cattttagaa
    30661 taggataaca aaggagaaag tcttgttgct ctctccttgt tgtatttatt aaattatcag
    30721 aaattttatt gacagattag aaaaataatg ttacatttat atccgcagat atctttcgat
    30781 accaaatcta catgaaagga cggggtatga aactttctca ttatttaatt ggcttacttc
    30841 tactcctagt ctttctctct attagcattg ggaccagtga tttttcatgg ggaaagctat
    30901 ttgatttcga ccagcagacc tggctcctct ttcaagagtc ccgtctccca agaactatca
    30961 gtattctcct gactgcctct agtatgagta tggcaggcct tctcatgcag actattaccc
    31021 aaaatcagtt tgctgcaccg agtacagttg gaacgactga agccgccaaa ctgggaatgg
    31081 tgctgagcct ttttgtcttt ccatcggcta gtctgaccca aaagatgctc ttcgcttttg
    31141 tttcatccat cgtattcacc ctcttcttcc tagcctttat gaccattttt actgtaaagg
    31201 aaaggtggat gttgcctctg attgggatca tctatagcgg gattatcggc tcagtcacag
    31261 aagttatcgc ctatcgtttc aatctggttc agagtatgac tgcctggacc cagggctcct
    31321 tctccatgat tcagacccat cagtatgagt ggctcttctt aggcctcatc atcctgataa
    31381 ccgtttggaa attatcccaa accttcacca tcatgaatct ggggaaagaa accagcgaga
    31441 gtttggggat ttcctactcc ctacttgaaa aactggccct ctttctggtg gcgctaacga
    31501 caagcgtcac catgattacc gtgggtggct taccatttct cggagttatc gttcccaatc
    31561 ttgttcgcaa gcgctatgga gataatctaa gtcaaaccaa actcatggtc gcactggttg
    31621 gtgccaatct agttctggct tgcgatatcc tatcccgagt tctgattagg ccctatgagt
    31681 tgtctgtcag tctcttgcta ggaatcatcg gtagtctcgt ctttatccta cttctctgga
    31741 gagggggacg aaaagatgca gactaaaagc aaacatacca agctcttctg gattctcatt
    31801 attcttgcca tcggagcttg tcttctctac ttttggccca tcactcactt gtcagccttt
    31861 gcttggaagt tgcgttccca aaagatcatc gtttatctct tggtagccat cgcgactggg
    31921 atttcgacca ttagttttca aaccctgacg gaaaatcgct tcctgacgcc tagtattttg
    31981 ggaattgaat ccttctacgt cctactacaa accctactac tggtttttga aagcaagttt
    32041 cttcaacttg gcaaatcccc tatcttagaa ttcctagtct tacttcttgt ccagtccttc
    32101 ttctttctcg ccttacaagg ttacttgaag acactgatga agcaagacct ggtcttcatc
    32161 ctgctgatct gtctagcgct cagaagtctc tttcgaaata tcagcacctt ccttcaagtc
    32221 ctaatggatc caaacgaata cgataaactg caaaatagtc tttttgcctc ctttcaacat
    32281 cttaacactt ccatcctagc catcggttct ctgatcatcc tcgctttgac aatctttttc
    32341 tttcgaaaag cagtcgttct agatgtcttg cacctgcaaa gagaaacggc tcagatattg
    32401 ggactcgatg ttgaaaaaga acagaaagag ctcctctggg gaatcgtgct tttgacctca
    32461 acggccactg ccttggtagg acctatggcc ttcttcggct ttatgctggc caacctcacc
    32521 tacctgattg tcaaagacta tcagcacaag ttactcttta tagtggccat tctggttgga
    32581 tttattagct taaccttggg gcaagccttg attgaacgag tctttgcact ggaaattcgt
    32641 atcagtatga tcattgagag tgtgggtggc ttcttattct ttatcttact atataggagg
    32701 tctcgtcagt gaaactggaa aacattgaca aatccattca aaaacaggat attttgcaag
    32761 gcatttcgct tgaagtcagt cctcaaaaac tgacagcctt tattggtcca aatggtgctg
    32821 gaaaatcgac tctcctctcc atcatgagca gactaaccaa gaaagatcag ggagttctca
    32881 gtatcaaagg acgtgaaatc gagagctgga attcgcaaga actggctcaa gaactaacca
    32941 tcctaaaaca gaaaatcaat taccaagcca aattgactgt tgaagaactg gtcagttttg
    33001 gacgttttcc ctacagccga ggtcgactta gatcagaaga ctgggaaaaa atccgagaaa
    33061 ctctgaacta tttggaactg accaacttaa aagaccgcta catcaatagc ctgtcagggg
    33121 ggcaactcca gcgcgtcttt atcgctatgg tactggccca ggatacggac tttatcttgc
    33181 tggacgaacc actcaacaat ctcgatatca agcaaagcgt cagcatgatg cagattcttc
    33241 gacgactggt ggaggaactc ggcaagacca ttatcatcgt cctccacgat atcaacatgg
    33301 ccagtcagta tgcagatgaa attgtcgcct tcaaggacgg ccaggtcttt agcaagggaa
    33361 gaaccgatca aatcatgcag gctgacctgc tcagtcaact ttatgagatt cccatcacgc
    33421 tagctgatat caataacaaa aagatctgta tctatagcta gtaacataaa agctcaagtt
    33481 agagaacctt cagtctctta gtcaataaga ttaagagact ccctaaatcg ttatcacatt
    33541 ttaaaaagga gaaattatga aaacatccct taaactttat ttcactgccc tagtggccag
    33601 cttcttgctc ctacttggtg catgtagtac aaactcaagc actagtcaga cagagaccag
    33661 tagctctgct ccaacagagg taaccattaa aagttcactg gacgaggtca aactttccaa
    33721 agttcctgaa aagattgtga cctttgacct cggcgctgcg gatactattc gcgctttagg
    33781 atttgaaaaa aatatcgtcg gaatgcctac aaaaactgtt ccgacttatc taaaagacct
    33841 agtgggaact gtcaaaaatg ttggttctat gaaagaacct gatttagaag ctatcgccgc
    33901 ccttgagcct gatttgatta tcgcttcacc acgtacacaa aaattcgtag acaaattcaa
    33961 agaaatcgcc ccaaccgttc tcttccaagc aagcaaggac gactactgga cttctaccaa
    34021 ggctaatatc gaatccttag caagtgcctt cggcgaaact ggtacacaga aagccaagga
    34081 agaattggcc aagctagaca agagcatcca agaagtcgct actaaaaatg aaagctctga
    34141 caaaaaagcc cttgcgatcc tccttaatga aggaaaaatg gcagcctttg gtgccaaatc
    34201 tcgtttctct ttcttgtacc aaaccttgaa attcaaacca actgatacaa aatttgaaga
    34261 ctcacgccac ggacaagaag tcagctttga aagtgtcaaa gaaatcaacc ctgacatcct
    34321 ctttgtcatc aaccgtaccc ttgccatcgg tggggacaac tctagcaacg acggtgtcct
    34381 agaaaatgcc cttatcgctg aaacacctgc tgctaaaaat ggtaagatta tccaactaac
    34441 accagacctc tggtatctaa gcggaggcgg acttgaatca acaaaactca tgattgaaga
    34501 catacaaaaa gctttgaaat aagccacttc aaactcaatt taatactcaa tgaaaatcaa
    34561 agagcaaact agaaaactag ccgcaggttg ctcaaaacac tgttttgagg ttgcagatga
    34621 aagctgacgc ggtttgaaga gattttcgaa gagtataaaa ttttgacatt ctaaaacgca
    34681 tcctatcaag ttcactcaaa cctgatagga tgcgttttta tcattttgaa atctatttac
    34741 cccacctcat tccccttctt gatttcgttt aagggaaaag cggtctggaa cggggtcctt
    34801 acctgttttc gaccagggat gacaacgtaa aatccgagcc aagcccatca ataccccctt
    34861 aaaaccatgt ttttcaatag cctgaatcat gtagttggaa caagtcagct caaagcgaca
    34921 agagggtgga aagactggtg agataaaacg ttggtaaaag cgcacaggcg ctattaaaat
    34981 tcgtttcatt atttcttagt tacagccatg gtgtgtagtt ggctgatttc ttttttatta
    35041 agacgacggg attctcctgg acggagtcct gtcaagtcta ggtgtccgaa acgagtccga
    35101 gacaacttat ctacttggag accaacagct tcaaacatct ttttaacctg atggttacgc
    35161 ccttcatgga tggtcaactg caccacagag cgatttttga ctgggtccac tttgagaatt
    35221 tcataaacag ctggcttggt tttcttacca tcaatctcaa gaccacgggt caaggggcgg
    35281 agattgtcct tattgaccac acctttaaca cgcgcgacat aaaccttgtc aatctcatta
    35341 cgagggtgaa tcatctcgtc tgtaaagtcc ccatcattgg tcaaaatcaa gacacctgat
    35401 gtatcccagt ccaaacgacc cacagggtaa atacgctctt tgacattggg caagaggtcg
    35461 acaaccgtct tgcgaccctt atcatctgtc acactggaaa tcacaccgcg tggtttgtta
    35521 agcagatagt agaccttttc ttcgttgtag ataggttgac cttcaacttc gaccttgtcg
    35581 cctgacttga tagtggttgc tagttcacgc accacttggc cgttaaccgt caccaagcct
    35641 tgcttaatca gctcttctgc ttttctccta ctggccacac ctgcgtgggc aatatactta
    35701 ttgattctca tcttcttcta tcctttcacc aaataattgg ctttcttggg cttgaatctc
    35761 aagctcatca atcactggta attcttctaa atggtttatc cccatgtaat ctaggaaata
    35821 atccgtagtc acatagaggt tggggcgccc caatacttcc tttttcccgt cttcctttat
    35881 caggtcaaaa gcctgcaact ttgccaaggc tccactcgag ttaactccac ggatggcatc
    35941 aatttctatc cgcgtaatcg gctgtttgta ggcaataatg gacaaggtct caagggcagc
    36001 ccgagacaag ctctggttga taggcgcctt agagtattcc ttcaaaatct ctgcaaattg
    36061 aggcttggtc accaatctat aagcaccact tgtctcaatc aaagccaaac tggaatctgg
    36121 gtccttttca tacttctggg ctaattttcc taaactttgc tggatgcctg tcggtggcag
    36181 agagaggagt tcagctaact ggcggacccg aatcccatct tcacccgcta caaacaagag
    36241 cgcttctatt tttgctaaag tactcatctt tcctctctat caagtctagc tttgaggcac
    36301 ttgactttct tccttctttt ccatgagata gatatctccg aaactctcct cttgcacgag
    36361 gatcagctcc tgggttttga ttaactctag ggttgccaaa aagagggtga tgacctcttg
    36421 gacattctgg gcttccttga acaaatcctg caagcgcaat tgatctcgtc caatcaagga
    36481 ctctttcacg ataatcatca tgtcctcaat cttatactca tcccgcaaga tcgtcgtgtg
    36541 attttgtgca aactcctctt ttttcttggc taggatattt gaaaaagcca aaaagaggtc
    36601 aatggtcgtc ttgtcatgca caagctccgc atcttcgtaa atcaactctg tcggcgcttt
    36661 ggaataatac tgggcccgtt cttggtgctt ggcttccaag tgctcaccca agagcttgaa
    36721 cttgcgatat tcttcgattt gagagaggag gtcctgctcc aggtcatccc ccaagtctgt
    36781 cacttctgct accttcggaa ggagtttacg actcttaatc agcatgagct gactagccat
    36841 gaccatgtac tcacccgtca cttccagacg catggcctgc agggttgaga cataggctag
    36901 atactgttcg atgacttccg taatgggcac atcgtagata tccatctggt acttagaaac
    36961 cagatgcaag agcaagtcca ggggtccttc aaaatctttt aatttaatat ccattatcta
    37021 tatttttcta aggtcaggac tgtttttaat cctaattttt ttgcaatttc gtacaaatcg
    37081 accttgtttt ctatttgtct tagaataaac tgttcacgta agacttgagc tgataaggat
    37141 ggaaaacctt tctccttgac aaaagattct aactgacgaa aggcccactg acgagaatag
    37201 ggtttctctc ctctttcaaa aagataggtc tgccccatca agggttccaa ttctgaaagc
    37261 aaggccgtgg gaatggtgac aatcctctgt tgggaagcct tgctgattcg caacacctga
    37321 aaatccagat tgatgtccgc aaccttgatg gctaaaatct cactgggcaa aagccccatt
    37381 tctaggatta agagcgctag caagcggccc tctggatggt cgctttcctg ccaaaaagag
    37441 tctaggtata gaatctctgg cttttccgtc ttcttttcag cttgtttggc taattccaag
    37501 cggtaaaagc tgtccacctc tcctttttga tagagaaagt atagaaattg gttacaggcc
    37561 gaaatctttc gcttctgggc gctgattttt agattggcta gctgggcttg gtaaatcttg
    37621 agactggtct cagaaatccg ctcacctacc atgtctaaaa attgctccaa atcatactta
    37681 taggactgct tggaattgac agacaagccc tgcttttcct ctaaaaaggc tgaaatcctg
    37741 tctctcattg gcatcgaatc tcatattcct tactaaagtc atgcaagagg gcattaacgg
    37801 ccttgaggat ggacttgcgc gtaataatcc cttggaaaat accctctgca tccacaaccg
    37861 gtaagaagga ctcatctact agcttgtgca agacctccgt aatggtgaaa tcaggcgaaa
    37921 caaccgctac gtccgttttt gtcatatgaa cgatatccgt atccgccatg atttcttggc
    37981 tcaagtcatg ctccatctga taggccataa tatctctgag tccaatcgtc ccaacaaact
    38041 gtttttcatc tgtcacaacg ggaacacggg tataggtcat ctgactgagc aagagggtcg
    38101 catgatccgc attgtgggta tcaatcaaca cagctagatt tttagcaggg gtcaaaaaag
    38161 tttcctcctg ccccaacaag aaagtctcaa actccttggc aatcatcggc taaactcctt
    38221 ggacaaacct ggatacacct cgtgatctcg tgtcaaaaag tccactttga agtaactatc
    38281 atcaatctcc acacgagcat agagacattc tctgatggta cctcgtggtt ggctgataga
    38341 acctggattt agaaagagga tcttgccttc catccaagca cttggcacat gcaagtgacc
    38401 atagaggcag atagcggcct cttcctcctg agcccagtag tccaactttt gaaagttgaa
    38461 attgatgtca aacaagtgac catgagtttg gataatcttg gtcgaaccaa gctcagtcac
    38521 cagacgttct gggtagccgg cgtagaagtc catgttccct ttaacaacgc ggatgccctc
    38581 ccaaagtgga gaatccggac gtagttcaga atcgccgtta tgaaaaacgg catcgacttt
    38641 gcccacatag cgatcacgga cttcttccac aatcaagcta tcgccatggg aatcgctcat
    38701 tacaatgatg gtttgctttg ccatgatgga aatacctcca aaagtttctt aacggctaag
    38761 gcacggtgag attgactatt tttttcttcc agggttaatt cagctgatga ctcacctgtc
    38821 tctcctacaa ggaagagggg atcatagcca aagccatttt cacccttagg ttcaaagtta
    38881 atataacctg accagtctgc ttcaacaact aaactttcct tatttgggct ggctacgact
    38941 agggttgtgt ggaactgagc cgagcggtcc ttgagttcaa agaccatggc caattcgtgc
    39001 aagagtttgg cattattttc acggtcagtt gctcccacac ctgcgaaacg agctgaccag
    39061 acgcctggta agccaccaag gacatcgact ttgagaccag aatcatctgc caaaaccatc
    39121 ttgcccgtta attgagaaat ggtttctgcc ttaaggcggg cattttcttc aaaggtcata
    39181 cctgtttctg ctacttcagg caggtcaggg tagtcattaa gattttccac atcgtagcct
    39241 aacttatcaa agatagctcg gaattccttg gttttacctt cgttacgagt cgcaatcaac
    39301 aaggtttctc tgaccttgct tgtctcaaag aaatcatgaa cattgacccc ttctttaggc
    39361 aattctacat acaagagttg cccattttca accaagagca gggccccatg acgttgaata
    39421 acttccaagt ttcgtcccat ttcttgatta agcatgtcta ccacaaagga gaacaaacgg
    39481 taggtagaac catagcgtaa aacagtaact gaaagcggga aaccatactc ttcatctcca
    39541 aatattttgg agaattcaaa cagaggaaaa tctgtcttat agtcgctcaa actgttaacg
    39601 ccaccaaaaa tactataaga cccaacatac cagtcccggt catccttata ttcataaatt
    39661 ttatttgtca taattctaca tgctccacat gaatctcttt ttccagccat tcttcaccaa
    39721 tttgtgcaaa actttggcta ctggctgttg tgtaaaaacg gtgatggagt ggtccagcat
    39781 cgcgaccacg attgatttca aaataattga gtaagactga gatatcccgt acgcactctg
    39841 ccccactatc gatgagctga acctttggcc ccatcacatt ttggataata gggcgaagga
    39901 gtggataatg agtacagccc aaaatcaggc tatccacctt tccaaccaag ggacgcaggg
    39961 tttcatagac caccttcttg gtaacactgg ttgacagggc acctgactca accaagggag
    40021 caaacttggg acaggccaag ctctccacct gtaagtcggg atccagatca tggattttct
    40081 gacggtatat gtctgattgt accgtcatgg gcgttccaat cactcctatt ttcccacctt
    40141 gactggactt gatggctgcc gaagctcctg gcaaaattac acccaagaca ggaatatcta
    40201 gttgagcctt gatttcttcc cagacgaccg cagtcgcagt gttacaagca atgacaatca
    40261 ttttgacatc cttggtcaag agaaagttga ccagctgcca agtatattca cgaatttgct
    40321 cagcaggacg ggggccatag ggcgcccgcg ccgaatctcc aatatagacg atttcttcat
    40381 ggggaagctg gcgcatgagc tcgcgcacaa cggtcaagcc cccgacaccc gaatccaaaa
    40441 aaccaattgg tcgattatcc atacagtctt ctttctagtc ttttttctga ttgatcgttc
    40501 attatgattc cttgtactga acgaactgac cgacagcttg atagaatgtc aactacctct
    40561 ctcttaatca taatcaaata tcaatagaat gcaaggaaaa agtctcatcc ctaaaccata
    40621 accaacatag tgagaagtct ggaactttca tcccagactt ttccttattt attttttctt
    40681 tttattgtta gcaagggctg ccttttgttg gcggatgatt tggtggtaaa cttgttgtac
    40741 cttagcttcg cttggttttt gaccatttgc actcaaaaga gtacgaactg cttcagcatt
    40801 caaacgtggg ttgtcagcaa attctttttc gatttgctta cgaaccaagt acattcctcc
    40861 aagagctcct cctagaaaag ctagcacaat caatacaatt gctaaaagta aatccatcat
    40921 ttttctcctg tttctttttg agtccctttc attataccac aaactagcca ccctgaccag
    40981 tttgttctac actttcaagg atggaataga cagcaaaaag aaccctgctt ttctgaaaaa
    41041 gaggtctcct tgctaagttt atactcaatg aaaatcaaag agcaaactag gaagctagcc
    41101 acaggttgct caaagcaccg ctttgaggtt acagataaaa ctgacacggt ttgaagagat
    41161 tttcgaagag tattaattta cataaatagc cagtgtttga tagggtttga gtagaatttt
    41221 ctcagacact tctgcatctt catagtttga aatcaaaatc tgtccatttt ggtagactgc
    41281 tggcaagtcg atttccactt ctttggcata aaagttattg agcactagta acttttgatc
    41341 ctcaaactgg cgttcaaaag cgtagacttg tttgctatct tcaaaggctg gtttgtaatt
    41401 tccttctgaa atgattggca tttccttacg cagtcgaatc aagtcttgat agaaggtgaa
    41461 aatcagacct ttgatttcat tttctacatt gatatcttta taggatttac caactttcaa
    41521 ccatggagtg cctgttgaaa atcctgcatt ttcagaagta tcccactgca tgggaatacg
    41581 tgaattatca cgcgacttag cttgaataat ctggaaggct tcttgctgac tctttccttc
    41641 ttctaagagc atctgatagg cattaagcga ttcgacatcc acataatcag tcatagaatc
    41701 atagtctggg tcaatcatcc cgatttcctc acccatgtag atataaggtg tcccacgtga
    41761 caggtgaatg ctggctgcta gcatggtggc tccttccttg cggaagtttt gaatatcgac
    41821 aaaacggttc aaggcacgtg gttgatcgtg attgttccaa aagagggcac tccaaccgtc
    41881 tttatcactc atttccttac cccaactatg gtaaagactc ttcaactctt caaaatcaaa
    41941 gggagccaag gtccactttt gtccatcctt atagtccacc ttgaggtgat gaaaattaaa
    42001 ggtcatggat aattcctgac gatcaggcga cgaatagagg acacagtttt ccatggtggt
    42061 agaagacatt tccccaactg tcataaagct atcgtcagat ccaaaagtgg cttggttcat
    42121 catacgcaaa tagttatgaa cgatgggttt gtctgtataa gctggcttcc cttcattttc
    42181 aggacagtcc actgaaacct cgtccttacc gatcaaattg atcacatcaa atcggaaacc
    42241 tttgacaccc ttgtcgcgcc agaaattaac aaccttgaaa agctccttac ggacattgga
    42301 attgcgccag ttaaggtcag cctgggtctc atcaaatagg tgaagatagt atttcccagt
    42361 atccccgaaa ggcgcccatg cagaaccacc aaacttagac tgccaatctg ttggttggtc
    42421 ttggatgaag aaaaagtctt gataatactt atcaccagct agggctttct gaaaccattc
    42481 atgctctgtc gaacaatgat taagtaccat gtccagcata aagtcaatct tgtgctcttt
    42541 accgacacac accatttcct caaaatcagc catatcacca aaaagaggat ccactgccat
    42601 ataatctgaa atatcgtaac cattatcccg ttgagggctt ggatagaatg gattgagcca
    42661 gaccatatcc acacctagtt tggctaaata gggaattttt tcgataatcc cacggaaatc
    42721 cccaataccg ttttcagtgg tgtctttgta agattttgga tagatttgat agactacttt
    42781 tcctttatca agtgtcatct gtttctcctt ttctgataaa agggaggaag cagtcttccg
    42841 tccctatttg tgctatttca attatactca atgaaaatca aagaacaaac taggaagcta
    42901 gccacaggtt gctcaaaaca ctattttgag gttgcagata gagctgacgt ggtttgaaga
    42961 gattttcgaa gagtattaga ttcgtgtagc gaccatgaga gatgctccag cttggatcgt
    43021 tgtcggataa gttccaggaa tagtcgctgt ataagcatct tggttggtga tgataacagg
    43081 agtttctgtc accagacctg cagccttaat gacatccata tcaaaacgaa tcagttgctg
    43141 accaactgta acgtgatctc cttggactac aagactttca aaacctttgc catcaagacc
    43201 tactgtatcc ataccgatgt ggatgagcaa ttcaactccc tcgtcagaga caatgccgat
    43261 ggcatgcttg gtagggaaaa gaaccgtcac tgtcccatta actggagagg tcaactcacc
    43321 ttggcttggt tcaatgacca gaccttgccc catgacacct gatgcaaaaa taggattcgt
    43381 cgcttgactc aattctttca cttggccagt tagtgggctg ataatttcta ccgaagtaag
    43441 ttctactggt tcatggttca caaattctgc ttcttcttga gcaacgaatt ctgcctgcaa
    43501 gttcgtatcg ccctctgttt ttgtaaagag accagccttg cggaagaaga aagtcaagag
    43561 cattggaaca acaatcgcaa ctagcatagt tcctgcaaat ggcagcatgt attgaggttg
    43621 aatagagaga atacctggca aaccaccgat accaatagaa gccgcagtta cattaaaagt
    43681 aacggataac atgcctgcaa gggctgaacc agtcatccca gcaacaaatg gataaatata
    43741 ttttacgtta accccaaaaa gagctggttc tgtaacaccg agataggctg aaatggttgc
    43801 aggaagtgaa acctgagcct cacgctcatc atggcgatgc atgaaataat aggcaaacac
    43861 ggctgagcct tgagcaatat tagaaagagc aatcattggc catagggcag tgccaccagc
    43921 atccgcaatc aattgtgtat caatggcatt ggtcatatgg tgcagacctg tgatgacaaa
    43981 tggagcgtag agggcaccaa aaattgcacc gaagagccat ttaactggac cagttaaacc
    44041 tgccaagaca actgatgaaa gtccttgtcc aattgtccaa ccgattggtc ccaaaacagt
    44101 atgagccaaa atcaaggctg gaatcaatga caagaaaggt acaaaaatca tagaaatgac
    44161 ttctgggata tgcttgcgcc agaagatttc aagataagac agactcaaac ctgcaagcaa
    44221 ggctgggata acttgggctt ggtaaccgat acgattaaca gtaaaatagc caaaattcca
    44281 aacccagttt gccgcgatat cagctgctgg cgttgaagca accgcatagg cattgagcaa
    44341 ctgaggcgat accaaacaga ttccgagaac aattcccaaa atttggctgg ttcccatctt
    44401 acgagaaaca gaccaagtaa tccctactgg taagaactgg aagatagctt caccaggcaa
    44461 ccagaggaag tgattaaccc ctgcccaaaa ctgagaggat tctgtgatgg tcttgccatc
    44521 caacatcgac caatggacac cttccaagac attacggaaa ccgaggatca atccaccaac
    44581 aatcaaggct ggaataatcg gagtaaagat ttccgccaga gtggtcataa cacgttggac
    44641 cacgttttga ttactcttag ctgcagactt ggctgcttct ttggaaacac cctcaatacc
    44701 tgaaacggct gtaaaatcat tataaaagat gggcacgtca tttccaatga ttacctgaaa
    44761 ttgacctgca tttgtaaagg ttcctttaac agctggaatt gactcgatag ctttaacatt
    44821 agccttctta tcatctccta aaacaaaccg catccgtgtc gcacagtgag ttacggcagt
    44881 cacattttct ttgcctccga ttgcctgaag cagatctttg gcttcttgtt caaattttcc
    44941 catttgattc tccttatact tgtcaaagca agtttgattt gataagtata ttgtaaacga
    45001 taccaaactt gtctgcaact tgtttggtat aaaaagggtg tttttttgta aattttctag
    45061 cacttgtatg gtaaaataga tacaggtgtt cattaaacta gactaaaaac ctatttaagc
    45121 aggcaaaatg aagaaatacc aacaattatt taagcaaatc caagaaacca ttcaaaacga
    45181 gacttacgct gtcggagatt tccttcctag cgagcacgac cttatggagc aatatcaagt
    45241 gagtcgtgat accgtccgaa aggccctgtc tctcctccaa gaggaaggat tgatcaaaaa
    45301 gataagaggg caaggttctc aagtcgtcaa agaagaaacc gtcaatttcc ctgtatccaa
    45361 cctaaccagc taccaagaac tagttaaaga acttggactg cgctctaaaa ccaacgtggt
    45421 cagtctggac aagattatta ttgataaaaa atcctcactg ataaccggtt tcccagagtt
    45481 tcggatggtt tggaaggtgg tccgccagcg tgtggtggat gatctggtat ccgttctgga
    45541 tacggactat ctggatatgg aactaattcc aaatctcact cgccaaattg ctgagcagtc
    45601 tatctattct tatatagaaa atggcctcaa actccttatt gattatgctc agaaggaaat
    45661 caccattgac cactcaagcg accgagacaa gattctcatg gacattggca aagaccctta
    45721 tgtcgtttcg attaaatcaa aagtctatct ccaagacgga cgccaatttc agtttaccga
    45781 aagtcgccat aagttagaga aatttagatt tgtagatttt gcaaaacgca agaaataaaa
    45841 gactgagaca ccagatctca gcctttttct ttatcccaaa actaattcat cactgaccag
    45901 actttggcca ctatttttct ggaagagatg cataaggtct tcaactgtca gatgcttttt
    45961 ctcttctccc ttgacatcca ccactatctt gccttgatag agcataatga gacgattgcc
    46021 gtactcaatc gcatgattca tatcatgggt aatcatcaaa gtcgtccact gatggtgctc
    46081 cacaatcttt tgcgtcaagt tcatcaccat ttgactggtt ttcgggtcaa gtgccgcagt
    46141 atgttcatcc aaaagtaaaa gttttggttt catcagatct gtcatgacta gagtcaaagc
    46201 ctgtctttgt cctcctgaga gatattgagt atctactttt aagcggtttt caagaccaat
    46261 attcaactcc ttcaaggcct cttggaactg gattctatcc ttctccttca cgccccaacc
    46321 aagccctctc ttctgcccgc gtctcaaggc aatagccata ttctcctcaa tcgtcaaacg
    46381 agaagctgtt cccatcttag gatcttgaaa aacacgggca atatccttgg ctctctttct
    46441 gacactcaga tttttaatgg atttccctgc cagtagaagg tccccttcgt ccaccgatag
    46501 attgccagcc aagatattca tcaaagtgga ttttcctgct ccatttccac caatcacaga
    46561 gataaaatct ccctcttcaa cctctaaatc taatcctttg aggacatggt tctcattgac
    46621 cgtccctgct tcaaatgtct tgtgaatatt ttcaagtgtt aacaaacttg tcataacttt
    46681 ctcctcctat tcatttctca atttcagacc acgaatcttt aatctctttt gcaattctgg
    46741 tgcaaataaa accaaagcga gcaagattgc attaaagaga cgtaccaagt tttgatctag
    46801 atttggaatt tcatagatat ttaaaataat caaacggtaa acaatagcac cgattccgat
    46861 ggataataag cggcctccaa tggtcaagtc gtgtatcaag acttccgcaa taatcaccga
    46921 gctcaaacca acaacaatgg ttcctgtccc agaagtcaca tctgaaaatc catcattttg
    46981 ggcaaacaag gaaccacata gagcaatcaa accgtttgaa atcatgtaac caacaatctt
    47041 catggtgtct acattgactc cattggcctc actcatcggg atattatccc cagtcgaacg
    47101 caagaccaag ccaatctctg ttttcatcaa aagggtcaag accaaacaaa caagtaaaaa
    47161 acaggccaaa ctaagtgaga aaacagttcc ttcatttgtc agacccaagc tcgccaactg
    47221 tttaaagacg gttgcagaat ctcccaagga aagattgggt acactgccca tgattttaat
    47281 attgattgaa tagagccctg tcaaggtcac aatccctgtc aagagagctg ggattttcat
    47341 cttggtgtgg agcattcctg atacaagacc tgctaccata cctgccagca aagcaagtaa
    47401 agtcgcaagc caaggatttg cccctgcctg tatctgtgat acaacgactg ctgcccccag
    47461 aggaaaggct ccctcagcag tcatatccgc aatatccaaa atacggaaag tcaagtagac
    47521 cccaatcgcc acaatcgacc acaacaaacc ttctgataaa ctagataaca caaaattcat
    47581 cttaatcctc cttattcctt gccttctaac ttactaatat caattcccaa tgcatctgcc
    47641 atttcttgat tggtatgcaa ttccagtttt tcaggcaact caactgctat attttctggt
    47701 ttctcacctt ttaaaacacg aatcagcatg cgagctgtct gacgtcccaa ttcttcataa
    47761 ttggttccgt agttatacaa gccaccaaca gcaatcattt ctgttgaacc accgaatact
    47821 ggaaccttgt gtttaataga aacctgctta actgtttcca tggttaacga aatgatatta
    47881 tcagttggga caaaaaccat atccacctct gccatcaaac tctctgctgc cgccttgacg
    47941 ttattactgt ccaagattgt tttttcaaca acggtaaagc ctttttcttt cagaagtcgc
    48001 ttagcttcat ccttttgaac aaccgaattg ggctcgctct gagtatagag gattccaatc
    48061 gttttagcct ttggcaacac tttctttatc aaattgattt gggttgaaat ggcatctgat
    48121 gactgatcgc ttgtcccagt tacattgccc ccaggatgtt ctcttgactc aaccaacttg
    48181 gcactgactg ggtctgttac agctgaaaag ataactggtg tcgtttgcgt cgtattggcc
    48241 aagctctgag cagaaggcct tgcgatggct agaacgacat cgctggattc tgctagttgc
    48301 tgggaaatcg tttttagatt tctttgttct ccctgtgcat tttgcaaatc aatctcaata
    48361 ttttccccct caacatagcc ttgtttggcc agcttatcca caaatccttc tctagtagcg
    48421 tataaggact ggtgggtaat aaattgcgaa ataccgatac gaaagacatc tttttcttat
    48481 ctgccttcaa ctgatgcaaa ctgatcagag aagtcaagag gagccccact accaagaggg
    48541 ggctagtgat tttcgaacaa ctttcataat gaaactcctt ctcagaaaag ataaaacaaa
    48601 aaaccgccta gatactatct aaacggatac ttgaaataac ctaacaagtt acaacttagc
    48661 tagtccattt agatattcat ctatatggac cacaatcaaa aatcttagcc acatagatac
    48721 aaacaaattg cttgaactgt ttgcatccat ggtgacatgt ctacaaacac tatctaaagt
    48781 agctaaagta aaagttttga ttcatagtta cggcttgtct cttattcatt tctttttctg
    48841 ctttcttttt tgatgcttcc tgtcctacca cctttttcat cacctgtcaa taacatttca
    48901 taatttttaa aatttttcaa aaattctttc aggaagcttt caaagttttt caaatcagtc
    48961 caacaaaaaa agttttataa tttccataaa aattgacatg gaaattataa aaccattact
    49021 agtttagtcc tttttgataa cgtgccaatt cggcttggtt cgcccaaaca tagtgacctg
    49081 gacggatttc taccatagat ggcttatcag tctcatagtc gtgttgactt gggtcgtaaa
    49141 ccttcaagac cttcttacgt tccaagattg gatctgggat tggtaccgct gaaagcaagg
    49201 cttgagtata tgggtgaatt ggattgttaa acaattcttc tgtttctgca acctctacaa
    49261 taacaccctt gtaaataact gcgatacgat ctgaaataaa gcgaacaacc gacaagtcat
    49321 gggcgatgaa gagataggtc aggccgagct ctttttggaa ttttttgagc aagttcaaga
    49381 cttgggcacg tacagaaacg tccaaggctg aaattggctc atctgcaata acaaagtctg
    49441 gttgcatgac caaggcacgg gcaataccga tacgttgacg ttgaccgcct gagaattcat
    49501 gagggtaacg agtcaagtgc tcagcaagaa gacctacttc acggataata ttttgaactt
    49561 tctctttacg ttcttcttca tccttaaata aacggtgatt gtaaagacct tcagaaataa
    49621 tataatcaac agtcgcacgt tcattcaaac ttgcggcagg gtcttggaaa atcatctgga
    49681 ttcgacgaat caattccgca gcttgttcac gcgatttctt accattaatc ttttgaccat
    49741 caaaaatgat atctccatta cttgtatcat ttagaccgat gatagcacga ccaatagttg
    49801 ttttcccact accggactca cctacaagcg agaaagtttc tcccttgttg ataaagaagt
    49861 tagcattttt aaccgcgaca aacttcttac ttccttcacc gaaggaaatt tctaaatctt
    49921 tgatttctac taatttttca gacatttcct tcctcctagt cagccagatg ggcaaatccc
    49981 attttttcac ggatcttatc atggagattt gcaatcacag ctggtttttc tacttttgga
    50041 gcatcctcat gaagaagcca agttttagcc caatgtgtct ctgatactga gaattgagga
    50101 gctttttgtt cgaagtcaat ctgcattgcg tagtcagaac gcaaggcaaa agcatcccct
    50161 ttcaggtcag tataaagtga cggaggtgtt cctgggattg agtaaagatc ccctttatca
    50221 tcagcaagct gaggcaagct agacaagaga ctccatgtat atggatggcg agggtcatag
    50281 aagacttcct caaccgttcc atactcaacg atttctcctg catacataac cgctacctta
    50341 tccgcaatac ttgccaccac accaaggtcg tgggtaataa agattgttgt gaaatgatac
    50401 tcgttttgta aagattttag caaatcaata atctgagctt gaatagttac atccaaggca
    50461 gttgttggct catcacagat caagacatca ggtcggcagg caagggcaat cgcaataacg
    50521 atacgttgac gcattcctcc agaatattgg aatgggtatt cattaaaacg tctatctgcg
    50581 tctggaatgc caaccttatt catgtagtca atggccaatt ctttcgcttc tttagctgtt
    50641 tttccttggt gttttacaat aacttctgta atctgactac caattgtttt aatggggtcc
    50701 aaactagtca ttgggtcctg gaagatagtc gcaatcttag caccacgaat ttgttcccaa
    50761 tccttgtgag aagataaagc tgtcaagtcc tgaccacggt agtcaatact accttgggca
    50821 atgcgaccat tttcttcgag catacctgtg aaggtctttg tcaaaacaga tttacctgat
    50881 cctgactcac ctaccaaggc taatacttct ccttcgacta gttcaaggga aacgccgcga
    50941 atggctgtca atactttgtc acgaacgtca aattccacga caatatcgcg agcagtcaaa
    51001 attacatttt tttcttttgt catttctact cctatctatg tgtacgtgga tcactagcat
    51061 ccgctaagtt ttgaccaact acgaaaaggg acaaggatac caagacaagg gttgtcaatg
    51121 gaatccagaa caagtaagca ttggttgtta cgttttgtga ataatccgaa atcaaacgac
    51181 ccaaacttgg cactgtaatc ggtaatccaa gaccgaagaa agacaagaag gcttcgtatg
    51241 agataaagct tggaagcatt tgagtcatgg ttgtcacaat aacagatacc aattgaggca
    51301 tgatattttt ggcaacaatc ttcaaggttg gtgttcccaa agtacgtgac gccaagttgt
    51361 attccaagtc acgatagcgc aagatttgca cacggatcat gaaggcaata ccaatccatg
    51421 ttgttacgct catggcaaaa atcagattcc agaatccagc tccgattgag taagtcaaga
    51481 caataacaat caaaagaggt gggatgtttg agatgacgtt gtaaacttcc atcatgacac
    51541 ggtcaactga ttttgaaata ccccaaatac caccgacaaa aacaccgata accaagttaa
    51601 tcactgtcgc aatcacagaa atgaggatgg agttacgagc tccgaaccag acaccgtcaa
    51661 agagcgattt accgttactg tcagtaccga accaatgctc cgcatttggc ttgatataac
    51721 gaacactaaa gtcgtttacc ttgctgacat cattgaaatc aaacttagaa aacattgggt
    51781 agatgaaact tatcaaaatg atggctacca agattcccaa catgactaca gttgattttt
    51841 tcttcataaa ttgtttaaac actgatttcc agtaagaata tgctggcgca tcaatagttt
    51901 cagaggcaaa atcgtcacgt tttacaaact gaaatttttc tttatcgatt gtagacatta
    51961 tttgcctcct ttctcagtca atttaatacg tgggtcaata atagtcatcc aaatatctcc
    52021 caaaagacgt gagaagatag aaatacatgt aaagatgaag acaagaccaa cgaccataga
    52081 gttattagat gcttttacag agtcaatcaa cattttaccc atacctggga aggcgaagac
    52141 tgtttcagta agggttgcac caccgataac cccaataatg gcaccaggaa ttcctgaaac
    52201 cagcggaacc atggcatttt taaagatgtg tttgtttgaa atttcttttt cagacaaacc
    52261 ttttgcacga gcgaaacgaa caaagtcttg agattgcaag tcaatcatgt aacgacgaat
    52321 ccaaatggct gtaccaggag cacccaacaa accaaggatg actgctggta aaacgtaaga
    52381 acgccaatct ccagctccca agatagggaa tgaatctgga agggaaatag atgatccaat
    52441 caatcgaacg atgtaaacca aggcaatcgt tggaagagca agcaagaagg tcaaagcccc
    52501 tgttgagagg ctatcaatcc aagtgttctt gaaacgagcc atggctgaac caagtggcac
    52561 ggcaagagca taggcaagaa ccaaaccaat caaaccagta atagcagagc tgacaatcat
    52621 agatggatat tggtaattac tttcagtcgc tgtataagga tcatctttcc catagctagc
    52681 tacttcacga gagtcagcct gactaggtga cttgtaggtt cttgagtaaa tatttacaga
    52741 agacgttttc ttacctgttg ggaactgaac ttgggcagtt ttggtttgtc cttgaccttg
    52801 agtaataacc tgaagaactg gtgtattagc ataggttggg taagagtcac ctaaattcaa
    52861 gttcacaaag ttttgatgaa caaatgggaa ctgactgtta aagtacaaga gatatttatg
    52921 tttagttcct gaaccgacca atgaccatcc gatagctgga tcattttcaa aacgaaggta
    52981 gcgtttcaag tctggatttt cagggtcttg gattttattt gtatggtcaa tgtcaatcaa
    53041 gttagcatag aagtgaaaaa cacgttcaaa aattggaatt tcacgagtag catagaattg
    53101 accactttca gtaaattctc ccaaagtcca accatgacct aattgattga tgtacttttc
    53161 ataaatagct ttattggtcg catttgcttc tactgttaca gaagaatcca tgctacttgc
    53221 cttttcttgc aactctttag tatcgtaata ctcaatgtag cccatacgct caaacacagt
    53281 attttcatag ttatcacgtt tatcagccgt tgtcgcaatt ttattatagt taggatcctg
    53341 cttgaaaatc aattttcgag gaaccaaggt atagataatc gtgtaggtca aagtcgttac
    53401 taagaaaatc gaaaccaatg accgcaaaac acgcataaaa atatattttt tcatattatt
    53461 tcctttaaaa atcccaaaag aaccttctcc tcatggagag aaagttctat tagaaattat
    53521 ttacttcaca tgacttgcca attctttttg agctttctca tttgattcag ctttttcttt
    53581 caaccatttt tcacgagctt tttcatactc ttccttagtc accgctttat cttgtaattt
    53641 caaatatttg aagtaaacat ctgacccctt agagcctgtt tgcgcagaag ctccagtaaa
    53701 tggaacaatt cgtgaaagca ctggtgctgc accagaagaa gccatagcag gaataaagag
    53761 tgaactatct gtcaaccatg cttgagccgc tgcatatttt tcataacgga cattcaagtc
    53821 gcttgtctct ttggcagctt catcaactaa tttatcgtat tctttcaaac caacttgaac
    53881 tactgaaggg ctatttggat tatcaaatcc taaatatgtt tttgtagttt cactgctagt
    53941 tgtttttaaa atatccaggt aagtagatgg gtcttgatag tctggccccc atgaaactcc
    54001 tcccgataca tcccaatcct cagatgaagc attggcagca tagtaagtaa tattaaggaa
    54061 ttcatcactt gtcatttgtt gaatatcaac aacgacattt tcaacaccaa gaactgtttc
    54121 tacagattgt ttaaaggact gaatacgaga tatatagttt tttgatgctt ggtctactgg
    54181 aacgtccaga tgaataggaa actgaacgcc gtctgcttct aaagctttct tagctttcgc
    54241 aaactctgcc ttggccttgt cagcattgaa taaaccatcc tgcccatcag ctaaattcac
    54301 acctttccac tcatcaccat aagcaggaag ttgagcagcg actaaatcac caaaggtctt
    54361 ctcaccagct gaaacaaagt ctggttttac aaataaatta cgaactgcta aagctgctcc
    54421 atctttacca ttgatttgag ctgagtaagc tgagcgatca agagcaaaat tcaaggcttg
    54481 acggaaatct ttgttaagca atgccttctt agtagctact ttctctgaat ctgtagtttt
    54541 agaagtatag ttgtaacttt ggcgatcaat attcacaccc agaccagcaa tcccagagcc
    54601 tgattgtgtg taatagatat tgtccttgta ttcttctgca accttagaat agttggagct
    54661 ggtagggtaa agacgggcat aactataagc tccactagtg aagttacgct ctagcgactc
    54721 ctgatctgat ccatcatagt aagctagatt gatagtatct aggtggacat tttctttatc
    54781 ccaatattgc tcatttttta caaactctac agaagatttt gcagtcaacc ctttcaacaa
    54841 gaatggacca ttataaagca aggatgtcgg atctgttggt ttagcaaaat cgcttccttt
    54901 tgatgtttcg aattcttcat tcagaggcca gaaaatagaa taggtcaact tagagttcca
    54961 gaacggttca ggctggttca aagtgtattg taacgtataa tcatcaaccg ccttgacacc
    55021 aactgttgaa aaatctgttg aagttcctga tagataatct gccaagcctt taaccgaatt
    55081 ttcagctaaa tacatagctt ctgatttttt atctgctgcg tgttttaaac cgttcacgaa
    55141 atctttagcc gtcacctctg catattcttc tccatcagag gtaaaccatt taaccccttt
    55201 acgaatctta taagtgtagg tcaaaccatc cttagagact tcccaatcct ctgcaactgc
    55261 aggagcaaga ttaccgtaat tatcgttagt gaataaacca tcaatcccat ttgaagtcac
    55321 tactgttgta ctatttttac ttgaaatcag gtagtccaag gtttctgggt ctgctgtata
    55381 aacatagcca taagctttag gggctgatga atcagatgat tttgaagaac tgcatgctgc
    55441 aagtacacct gctgctaata aaacaagacc tgctgtagca aatacacgat tttttttcat
    55501 tttctactcc tctgtttatg tgaattatag attgacaacc attatatcac attatccatt
    55561 aaaaatcaaa caaattttca gaatatttag gcttgttggc acaaattttt catttttttt
    55621 gaatatatga ttcaaattgt cgttcgaagt gtcaaagact acagtgaaaa taggaaattt
    55681 gacacagaaa ttttggagct taggaagaca tacagtaaaa tgaaatacgg acggaacaat
    55741 gtgattttgg aattcaaatt aaattataac aatattgtag aagtatcatt ctagtattca
    55801 agattcagtt tactatgtct tttcacacca accttattcc gaattcaatt actttcgtga
    55861 tttacacata tagattaaga ctatctttta tactttaaaa tttctcgcta ccttgtccac
    55921 tatatgctcc tcgctatcac gtttctattc atagcctacg atttcactat tgctttctct
    55981 gacaattctt atttcctgcg tcagacttaa aacgatctat ccccagacca ttttaatccg
    56041 ctacctcacg atagtcaggc ttggggagcg ctattgtatt caccggtagt ggatccctac
    56101 agaggactta cacctcagat gcacgacatg cccatcgtat aaaaaaatct cctacccaag
    56161 gtagaagatt tcaaacttat aaaacttaat ccgtcatgtc cgataccaac attcgatgct
    56221 ccaatggaat actgcacata actagcaaga aaataaagcc tgactgaatc cagaagagag
    56281 ccaagtcaaa aattccgtgc acagcaacca ctgtaaggaa agatagataa aggccgataa
    56341 tcggacgttt ccccgactcc tgactcatat ccatcatcaa gcgaacagga gcaacagaag
    56401 acaaaactaa taaaatggta cccacaattc cgtaactcag aatcgtatca atataaagac
    56461 tgtgggcatg ttcatgataa ggagcatgta tccgaggata agagtgcata taggtcaatg
    56521 gcccttcacc ccaaaaagga ttttgcttaa acaaggccat cccagcatcc cagatagaaa
    56581 tgcgttcttc catagaagag tctaaagtac ccattcgaac tcccaaatca ctagaaaaga
    56641 ggaaactcaa accaatcgcg aagaccccaa tactaagcca aaaggccttc cagtttttaa
    56701 tagtcgtaaa gagatagata attgctccag cgataatagc aggaaaggca gttcgatttt
    56761 gagtaaagtt caaaccaaag agattaacaa agcctgcaat cacacagaat actttcaacc
    56821 aattcaactt ggtcgttgta aacagataga aagcaatcat aatacagaaa caacaaataa
    56881 ttccataata attaggatta aagaaggtca cttctgcccg gttctgatgc cacacctgca
    56941 tattgggtga aagaaaagca tagttaaatt tcttcacaat ttggaaatgt tctaaactgg
    57001 caaaagcagc tgacaagaca ctaccaaaca agacgaactg caaaatcaat cgaaagaatt
    57061 tatgggataa aatcgactga tagtgcaaaa agaaaatagt aaatagaaac attcctactg
    57121 aagccacaag acccatccaa ttttgtgcaa gaatggatat aacagtacta tagctaagaa
    57181 aaagaagcag catcggatgc tcccccattt tctgaagaat acttttcatg tctcctgtaa
    57241 aaatcaaact gataatatat aaacagagta caactacaaa aagataaaag ggtaaaaaga
    57301 tacttaggat aattcccaat aaaatcagct ctttactaga caaccccttc agcttttcaa
    57361 taaagcctat tgatttcaaa atgaatcctt tctctccaaa tcagctgatt cagataatag
    57421 taagctatcc tatattgtac cactttttta gcaatttgaa aacaaaggaa acgttttcca
    57481 aaataaaaac cctattttat ccaccatatc aaggcttcaa aatgatactt caactccatt
    57541 ctcaattacc cgataagtct gattttgcaa atcaatttct actactgctg ttacggactt
    57601 atctttattt tgacgtttga ttacaatgct gtgagctgtt ggtgtctcta tctcagtagt
    57661 cccttctaga tcaaaggctt ctgaacggtt acggaaagaa aatagattga gaagggcctt
    57721 cacaacaggt cgttgcactt cttttgctat ttcctcgttg ctatagtaat gacgattaat
    57781 atttcgacct tctttagttt cttctaataa tttcaagtca ttcttgcctg ctaatagacc
    57841 cacatagtaa acctgaggaa tacctggggc aaaagcttga attagacgag cgagaaaata
    57901 cttgacatca tcatctccaa gcgctgaata gtaggttgaa ttgatttggt agatatctaa
    57961 gttgttatac tcggcactag agtacttacg tttgacattg gctccaacct tatagagttc
    58021 atttgaagca tagtcaatct cctcatcggt caggatatcc ttgacatcta ctactccaat
    58081 cccatcatgg gtatctagca tcgtaaattg cttcatcggg ctcatcttta accacttagc
    58141 caaacgctct gttctggaac tgtaaagagt ataaagtgtc accattggaa gagcaaaatc
    58201 ataaacatag taatcatggt ctgctatttt aaactgaatc gaatagtgtt catgaatctc
    58261 aggtaaaagc tctgtcccat actcagcagc gatatctcga actttgtcca ataaatccca
    58321 aatatctggt tccacaaaga aatcattagt atccaatttc ttcactgcat aagcaaaggc
    58381 atctagacga atcaaatcac acccattact tgccaagtgc tgaatggtct tacggataaa
    58441 ttccatagtt acttctttgg tcacatcaag atcaatctgc tcctcaccaa aggtattcca
    58501 caaatgttcc actgaaccat cttcaaacac aatctcttgc tttggtgcac gatccttacg
    58561 cttgtaaatt aaatctacat cagactgtgt cggacggttt tctggccaaa acttatccca
    58621 gtttaaaaag agagctttaa attcactggc ttcatgtttt tcttgatagt ccttataata
    58681 cttggattga cgagaaatat gattaatcat aaaatcaaac ataagataat atttctcacc
    58741 taaacgctta acatcctccc aatcaccaaa agctgagtcc acttcgtcgt agtcaactgg
    58801 cgcaaatcca cgatcacctg ttgatgggaa aaatggtaaa aggtgaactc ctccaatagc
    58861 atctccaaaa tgctcttcca aattatcata taagtcttta agattatttc caaggctatc
    58921 agaataggta atcaacatgg ttttattttg aattggcatc attactctcc tttttctaat
    58981 tgaagccaag tctcatatga tctggcttca taaataaaat tcattttaaa tctctattta
    59041 tcatcaaact cgtactaata tagactgtga taaacaaagt actactttct tgttttctgc
    59101 atagaattat caacaagcta aactcttcct ctgtgtcaaa gactatagat tccatgagct
    59161 cttcgaaaat ctcttcaaac cacgtcagct tcaccttgcc gtactcaagt acagcctgcg
    59221 gctagcttcc tagtttgctc tttgattttc attgagtatt acttcactgc cccgttgctc
    59281 attcctgaaa tgatatggcg ttggaagaag agatagacaa tggtgatact gataatgccg
    59341 accacgtaag aggcaaagct tggtccgtag tcgttgaaat attggcctgc gtagttgtat
    59401 tggaacaaag gcagagtcca cattttggaa tcccggttca agacaaggag tggcaacatg
    59461 aagtcattcc agaaccaaag ggcattgata atcatggttg tcgcatgcat cggtttcatc
    59521 attgggaaga tgatgcggaa ataggttgta aattgattag ccccatcgat ctctgctgct
    59581 tcatccagac tttctggaat cgagattttg atatagccaa catagagaaa gagggtctgt
    59641 ggaatcgcat aggtcaagta gagcaagatc aaaccaaagg tattagccaa accgagttta
    59701 ctcatcataa ccgtaatcgg aatcatgatg acttggaaag gtacgaagat tccgaggatt
    59761 aagagggtat acatgatggt aaaggctttt cttttactca tattgcgagc gatggagtag
    59821 gctgccatag ggataaagat cattactgca agtaaagaca agacagtgat gacgacagag
    59881 ttccaataat agcctccaat cccatcagct aagagacggc taaagttgtc ccatgtgaag
    59941 ttggttggaa agccaaagaa attatctaca atatccttag tgggtttgaa ggaactaaag
    60001 agggtagcaa ggagcggcac taaaatcaga accgatccta gaatcaatag aatgtatttg
    60061 ccaatcaggg cttttctttc atcttgtttc atcatgcttc tcctcttaaa tttcaaattt
    60121 cttagatact ctcaattgga tgatcgaaat cactacaatt aagaggaaca agattacggc
    60181 aatggcattg gcataaccga attggttgtt tttaaaggca tagttataaa ccaagagccc
    60241 aagtgaggtt gtggcattgt ttggaccacc accggtcatg gcaaagactt ggtcaaaggc
    60301 agtcagccca ccttttaggg ctaggataaa gaccatagag acacttggta gcaagtaagg
    60361 caattcaatg ttccagaaaa cttgcttgct agtcgcacca tcaatccttg ctgcctctgt
    60421 aatctcagtt ggaatagatt gcaaaccagc taggaagatg atgatgggca tagccacccc
    60481 ttgccaaaga aggacaaagg cagccgcaaa gattgctccc cacttagtcc ctaaaagact
    60541 ggtttggaga aattcaatat gaagggcatt cccaatcgct ggaagaccgt agttgaagac
    60601 ttgcttgaag atcaaagcca ctgtcaaacc agataaaaca gctgggaaga agaaccaagc
    60661 acggaagaag gtttggcctt tgattttaga gttcaagaca cgcgcaatga agatcccgag
    60721 tgcaatctca ccaaccacca tggcaatcgt aatgattgcg gtaaagccaa tcgcattcat
    60781 gaattttgga tccatgaaga ggagcttaaa gttgtttaag ccgacaaatt tgtagttata
    60841 agtcaatcct gtccagttgg taaaactgta aaaggctcct tgaaacatcg gcacatagaa
    60901 gaaaattgct tgtaacaaga gggggatgac cacaaaagcc catgcccaat atttttgtaa
    60961 tacttttttc atagtctctc tactcctaat ccacatccgc tttcatcggg ttaaagaagg
    61021 cattcaaatc attgaccatg ccttgtttat caccggtcaa gacatagttc atggtcaagg
    61081 tatggaagtc tgcttcactg gtccagtatt gttgcaacca gaccaagtga cgatccgtaa
    61141 aggcatattc ggtcatacca gcaagcggtg aatcttctcc tgcttgtttg accccttcga
    61201 tcgctgttgg agatccgtcc acatcgtagt atttttgcat gacttctgga cgggtcatat
    61261 attccacaaa ggcattggct tcttttggat gtttggtggt ggctgagata gaccatgcca
    61321 agtctcccgc accaacggtt aagctttgtc ctttttcttt tcctggaatc atgaaggtcc
    61381 caatcttaaa gttcggtttt tgttcattaa tcgctgtgat cgcccaagac ccatttggtg
    61441 tcatgaggac atccccacgt gcgaaggctc cgataacatc ggtatagcca gcaccttccc
    61501 agttcttttg cttagagcca ttgatgcgaa ggatgtccat gaccttgata tcatctttca
    61561 taatcggatc cgacaattta atggcatttg gttgagaata acgaaggtat tgatttgctt
    61621 cttttcctcc acctgttgct gtcgcaaagg ctaattgatt gtaaccattg agtgtccaag
    61681 catctgcacc tgcaattcca aatggtgttt gtcctttagc aacgatatct ttgactaact
    61741 gttcaaattc atcccaggtt tcaggaacct tcaagcccag ttcttcgaat ttatctttgt
    61801 tgtagtaaat tccataagca ttagctgtaa aaggaacgtt gtaaactttt tcgtttacag
    61861 catatttttc agcgtagcca tttttcacgc gtttcaggta gtctttgttg ctcaaatctt
    61921 caaaaacacc tgcttttgcc cattcttgca gttcgatgga ctgtgggtaa atattgacca
    61981 catcaggcac atctcctgcg agaacgcgtg tcttcaatac ttcaccagca tttggtacat
    62041 tgacgacttt gaccttgatc ttagggtttt ccttctcaaa atcacgagtg atttcttcca
    62101 aggttttggt catttctttt ttctggttga aatactcgat ggtcactgtg ccatccgcag
    62161 atttaccata gttggagcaa gcgccgagcc caaacaaagc taaacctgta gttgcaagaa
    62221 gtccgatttt tttataccat tccattagaa agcctccttt ataaatttat acacccttat
    62281 tgaactgcac cccaaaagtt agacagaata aatctaactt ttggggtcag tacatatcat
    62341 agttttctaa aaatatactg tctactcaaa aaatctcctt gggataagat aacagttaag
    62401 cccgcataca ttagttctgc acctgagtaa acttcgccat tttcctgtaa tttatatagt
    62461 ccctcttcat ccaaatcttt taattttaaa gttgtttcca tggtctctac aacagataaa
    62521 acgcgaacgt aggttacaat cgtttgattt ccgtaattaa attgtacagc tgcttcattg
    62581 gatgcagtat caggattaat tagtctatac tgctgtccta actgaactac tggtcgtaat
    62641 tctttataca agttcacctg attagcaatc gtagctttct cttcatctga taaatttgtc
    62701 aaatcaagct catagcccaa atttcccatc attgctacat ggccacgtgt ttctaatggt
    62761 gtcattcgtc ccatctgatg attcggtact gctgacacat gagcccccat agaaatggtt
    62821 ggatagagat aggatgaacc gtattgaatt ggtaaacgtg caatggcatc agtattatca
    62881 ctagcccaga cttgtgggaa atagcgtatc ataccaagat catttcgtcc accaccacca
    62941 gagcaggact caaagagaat atggctgtgc ttctctgtca gataagaaac gagttcgtaa
    63001 agtcccagca tgtactgatg agattgcatc tgtgtctcta gataagttaa tccattccct
    63061 agcttagtga tattgcggtt catatcccat ttaatgtaat caatctcatg ataagatagg
    63121 agttgatcta agacactttt caagtattct actacctgag gattggcaag attaagtact
    63181 aattgattcc gagaataagt atgctcataa tcaggaacct gaatagccca gtcaggatgt
    63241 tgacgataca aatcactatc tacagaaatc atttcgggtt ctaaccaaag tccaaactgc
    63301 aaacctcttt catggatagc tgaaatcaga ctttctagac ttccacccag tttttcctca
    63361 ttaacaaccc aatcacctaa agcacgatta tcatcaaaac gattgccaaa ccaaccatca
    63421 tctaatacaa aaagttcaat gccaactttc ttagcttcat ctgctaactc taacagtttt
    63481 tctctctgaa agtcaaagta agtagcttcc cagttattga ttagaattgg acgttctttt
    63541 ttagaaaatt cacttagagt aatgtgcttc agtacaaaat tctgactttc atgactaata
    63601 ccagttaatc cctgatctga atgagtcact aaagctaccg gtgtttcaaa gtattcctca
    63661 ggggctaact tccaagaaaa gttttctgga ttaatgccaa tagccacccg aacttcattc
    63721 aattgatttt tttgaacaaa agcttcaaag ttgccactat acattagttg aatagcaaac
    63781 acattcccag catcctctgt gactccttgt tcgcatagta gaagagctgg tgtttgagca
    63841 tgaccagaag cacctcggtt tgaactaatc gaaaagattc cttgttctac ctgttgacgt
    63901 ctaacagtct tttcacgagc ataagcaccc tgcagagtta ctatttcgta atctgcagct
    63961 ggaaaatcag ccataaaaga aaaatcttta tggatgacaa cttcctgatt actattatta
    64021 tctaatttac tgtagctagc aatagtcgca tcattattaa aagtagtata atacaaagtc
    64081 agactaagtt gagccttaga atcttctaac attaagacaa gagtctctgt atcgtccatg
    64141 ctatgtggag aaggtaagcc ctgtggacca ttctgacctt ttaaaatctt tgcttctaca
    64201 aatcgaaagt ctgttacttc agttacacta tgctgaacct gtatagttgg tttcctaaaa
    64261 tctcctaagc catgttgtcc aaaaatctgt cgctgagtat ctaaactaaa ggttcgatta
    64321 gtagccgttg gatttcctga aaaggcatgg tctcgttcat aaacactatt ggaaccttta
    64381 tagttcttaa tagtctttcc taaatgtttc aaaagtaagt agccatttcg attttcaata
    64441 atcaaactta gatttttact ctcaacataa aatagattat tctctatcct aactcccatt
    64501 tacttcacct catcacttta ttgattatat tttatcacct gaaatcgctt tccaaaatag
    64561 aaaaatgtct caagaatatg gtaaaatgtt aggtaggagg tagcacatgc tagttttttc
    64621 agaataccag actggaacaa ttgaccttgc cctaagcttt tatggatatg aggaatgcac
    64681 acctaattac tcttttggtc cagccattcg tgatacatac gtcctacatt acattagtaa
    64741 aggacaagga aaatttcatt acaagggtaa aattgttgat ttaaaagaag gagatttctt
    64801 tctattaaaa ccagaggaac taacctttta tcaagcagat agtaaagaac cttgggccta
    64861 ctactggtta ggaatcactg gagggaaatc ccctgattat tttgctcttt ctcaaatttc
    64921 tgatcaatcc tatctcatcc aatctgaaac ttgtcatacc cagactactg caaaactcat
    64981 ctcagacatt gtccgcttcg ctcagattac aaaatcaagt gaattagctc aactccatat
    65041 catgggacaa cttcatgaac tgatgtttca tctgggaact attgctccca atcagaaaaa
    65101 aaagaatatt tcatcaaccc accaactcta tcttgaatgc aaacgattaa ttgatagcca
    65161 ctatcctcaa tcacttacaa ttcaagattt agcaaaagaa ctatccgttc acagaagcta
    65221 cttatcaagc gtattcaaag aatttaatac cttatcaccc aaagaatacc tactctacgt
    65281 tcgaatgcac cgagctagac aacttctcga aaatacccaa gagtccatca aggtaattgc
    65341 atactcggta ggtttttcag atccactcca tttttcgaaa gcttataaac aatactttaa
    65401 tcagtctcca agtcatacaa gaaaagaata ctctcaatac caactagtga gaaaggcaac
    65461 attatgaaat cctaccaagc tgtctaccaa atcctatcta aagaaaccga ctatatcagc
    65521 ggagaaaaaa tcgcagaaaa actatcccta agccgaacat caatttggaa agctatcaag
    65581 cgactagaac aagaaggcat tgaaattaat agtatcaaaa atagaggata taaactaatg
    65641 aatggtgacc ttattcttcc agagattcta gaagaaaatc ttccaattaa agtcagcttt
    65701 aaacccgaaa caaaatcaac acaactagat gcaaaagaag caattgattt aggccatgaa
    65761 gcaaatactc tctatctagc ttcctatcaa acagcaggcc gaggccgttt tcaacgttcc
    65821 ttctactcac cacaaggtgg aatttatatg acactccatc ttaaaccaaa tctcccctat
    65881 gacaaattac catcctacac actacttgta gctggagctg tctacaaagc cattaagaac
    65941 ctaactttaa tagatgtcga tataaaatgg gtcaatgata tctatctaaa caatcataaa
    66001 attggaggaa tccttactga agcaatgacc tctgtagaaa ctggcttagt cacagatatc
    66061 attattggag taggtatcaa tttcactatt aaagacttcc ctcaggaatt aaaagaaaaa
    66121 gctgccagct tatttaaagc tacagctcct ataacaagga atgaattgat catagaaatc
    66181 tggcgtgctt tcttcgaaac accagcagaa gagctattat acctatacaa aaaacagtca
    66241 ttcattctag gaaaagaagt cactttcaca ctagagcaaa aagactacaa gggacttgct
    66301 aaagacatct cagaaaatgg aaaactttta gttcaatgtg ataacggaaa agaaatctgg
    66361 ctaaatagtg gcgaaatttc tctcaatagt tggaagtaaa ataacacaat tataatataa
    66421 acgatataaa aataacttca gattagtaat tcaattaagt tttacggatc tgaagtttta
    66481 ttgtctctaa aaacaaaaaa gagagttaca aactctcatt aaaacggaga ataagggatt
    66541 cgaacccttg cgccagttac ccgacctaac gatttagcaa accgtcctct tcagcctctt
    66601 gagtaattct ccaattaatg ggcacgagtg gactcgaacc accgacctca cgcttatcag
    66661 gcgtgcgctc taaccacctg agctacgcgc ccaagttaaa aaacttggta atttgaacaa
    66721 agttcaaagc gggtgacgag aatcgaactc gcgacaacag cttggaaggc tgtagtttta
    66781 ccactaaact acacccgcat aaatactatc aataaaatgg cgcgagacgg aatcgaaccg
    66841 ccgacacatg gagcttcaat ccattgctct accaactgag ctaccgagcc ttattgcggg
    66901 agcaggattt gaacctacga ccttcgggtt atgagcccga cgagctacct agctgctcca
    66961 tcccgcgtta atataaaagg aggatgtggg attcgaaccc acgcacgctt ttacacgcct
    67021 gacggttttc aagaccgttc ccttcagccg gacttgggta atcctccaat attcaaatgg
    67081 accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct aaccagctga
    67141 gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg acctcccggg
    67201 tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa gacaggattc
    67261 gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta cttcccgagt
    67321 taaaaagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc gtagtcaggt
    67381 actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa tcgaaccggt
    67441 acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc gccaccccgg
    67501 cctctctaag cgaacgacgg gattcgaacc cgcgaccccc accttggcaa ggtggtgttc
    67561 taccactgaa ctacgttcgc actgttttct tctatctaaa aatgccggct acatgacttg
    67621 aacacgcgac cctctgatta caaatcagat gctctaccaa ctgagctaag ccggctcatt
    67681 tgttatatct taatgcgggt taagggactt gaacccccac gccgttaagc gccagatcct
    67741 aaatctggtg cgtctgccaa ttccgccaaa cccgcatata tgacccgtac tgggctcgaa
    67801 ccagtgaccc attgattaaa agtcaattgc tctaccaact gagctaacga gtctaaaata
    67861 acttgcgtta tcttaaacgg tcccgacggg aatcgaaccc gcgatcttcg ccgtgacagg
    67921 gcgacgtgat aaccgctaca ctacgggacc t
//
LOCUS       DAVLRT010000003        63310 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLRT010000003 DAVLRT010000000
VERSION     DAVLRT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.003"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(122..1102)
                     /locus_tag="VXM85_000157"
     CDS             complement(122..1102)
                     /locus_tag="VXM85_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1344800.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHETMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1418..3433)
                     /gene="recG"
                     /locus_tag="VXM85_000158"
     CDS             complement(1418..3433)
                     /gene="recG"
                     /locus_tag="VXM85_000158"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1344801.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3452..4555)
                     /locus_tag="VXM85_000159"
     CDS             complement(3452..4555)
                     /locus_tag="VXM85_000159"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1344802.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVNGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHIKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4545..4913)
                     /locus_tag="VXM85_000160"
     CDS             complement(4545..4913)
                     /locus_tag="VXM85_000160"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1344803.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(4952..5983)
                     /locus_tag="VXM85_000161"
     CDS             complement(4952..5983)
                     /locus_tag="VXM85_000161"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1344804.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(5985..7016)
                     /locus_tag="VXM85_000162"
     CDS             complement(5985..7016)
                     /locus_tag="VXM85_000162"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1344805.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7097..9610)
                     /gene="secA"
                     /locus_tag="VXM85_000163"
     CDS             complement(7097..9610)
                     /gene="secA"
                     /locus_tag="VXM85_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1344806.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9756..10043)
                     /locus_tag="VXM85_000164"
     CDS             complement(9756..10043)
                     /locus_tag="VXM85_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344807.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10054..10704)
                     /locus_tag="VXM85_000165"
     CDS             complement(10054..10704)
                     /locus_tag="VXM85_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1344808.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(10708..11349)
                     /locus_tag="VXM85_000166"
     CDS             complement(10708..11349)
                     /locus_tag="VXM85_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344809.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            complement(11342..12133)
                     /locus_tag="VXM85_000167"
     CDS             complement(11342..12133)
                     /locus_tag="VXM85_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344810.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(12318..12653)
                     /locus_tag="VXM85_000168"
     CDS             complement(12318..12653)
                     /locus_tag="VXM85_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344811.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(12794..13129)
                     /locus_tag="VXM85_000169"
     CDS             complement(12794..13129)
                     /locus_tag="VXM85_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344812.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13220..14530)
                     /gene="der"
                     /locus_tag="VXM85_000170"
     CDS             complement(13220..14530)
                     /gene="der"
                     /locus_tag="VXM85_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1344813.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14544..15257)
                     /locus_tag="VXM85_000171"
     CDS             complement(14544..15257)
                     /locus_tag="VXM85_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1344814.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15254..16150)
                     /gene="dnaI"
                     /locus_tag="VXM85_000172"
     CDS             complement(15254..16150)
                     /gene="dnaI"
                     /locus_tag="VXM85_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1344815.1"
                     /translation="MESVGDVLKRQPSRFHYQDLVQKIMKDPDVAAFVQQESLNQDEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVMNHGYADVSYEETPELIAAEKEAA
                     IKKRLNLINFPSSLKNVSFLDVYRDDVQRLTVLKRMIEFVNDYPNNLKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSDVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16151..17320)
                     /locus_tag="VXM85_000173"
     CDS             complement(16151..17320)
                     /locus_tag="VXM85_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1344816.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     EQKAKTQKQNQTAPAKTNIPKWSNPEYKNTSSAEELEEMERQTLELLAKLDNGGD"
     gene            complement(17321..17794)
                     /gene="nrdR"
                     /locus_tag="VXM85_000174"
     CDS             complement(17321..17794)
                     /gene="nrdR"
                     /locus_tag="VXM85_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1344817.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            17934..18299
                     /locus_tag="VXM85_000175"
     CDS             17934..18299
                     /locus_tag="VXM85_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1344818.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18304..18999
                     /locus_tag="VXM85_000176"
     CDS             18304..18999
                     /locus_tag="VXM85_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344819.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19016..19783
                     /locus_tag="VXM85_000177"
     CDS             19016..19783
                     /locus_tag="VXM85_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344820.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20196..21395)
                     /locus_tag="VXM85_000178"
     CDS             complement(20196..21395)
                     /locus_tag="VXM85_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1344821.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGSMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21392..22285)
                     /locus_tag="VXM85_000179"
     CDS             complement(21392..22285)
                     /locus_tag="VXM85_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344822.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22401..22481
                     /locus_tag="VXM85_000180"
                     /pseudo
     CDS             22401..22481
                     /locus_tag="VXM85_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            22966..23179
                     /locus_tag="VXM85_000181"
                     /pseudo
     CDS             22966..23179
                     /locus_tag="VXM85_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            23594..24211
                     /locus_tag="VXM85_000182"
     CDS             23594..24211
                     /locus_tag="VXM85_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1344823.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(24531..25418)
                     /locus_tag="VXM85_000183"
     CDS             complement(24531..25418)
                     /locus_tag="VXM85_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1344824.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(25567..27450)
                     /locus_tag="VXM85_000184"
     CDS             complement(25567..27450)
                     /locus_tag="VXM85_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1344825.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            27827..29281
                     /locus_tag="VXM85_000185"
     CDS             27827..29281
                     /locus_tag="VXM85_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1344826.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            29262..30227
                     /locus_tag="VXM85_000186"
     CDS             29262..30227
                     /locus_tag="VXM85_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1344827.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(30262..31536)
                     /locus_tag="VXM85_000187"
     CDS             complement(30262..31536)
                     /locus_tag="VXM85_000187"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1344828.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(31536..32708)
                     /locus_tag="VXM85_000188"
     CDS             complement(31536..32708)
                     /locus_tag="VXM85_000188"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1344829.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(32839..33087)
                     /locus_tag="VXM85_000189"
     CDS             complement(32839..33087)
                     /locus_tag="VXM85_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344830.1"
                     /translation="MVSSWGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(33155..33778)
                     /locus_tag="VXM85_000190"
                     /pseudo
     CDS             complement(33155..33778)
                     /locus_tag="VXM85_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(33840..34289)
                     /locus_tag="VXM85_000191"
     CDS             complement(33840..34289)
                     /locus_tag="VXM85_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1344831.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(34291..35049)
                     /locus_tag="VXM85_000192"
     CDS             complement(34291..35049)
                     /locus_tag="VXM85_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1344832.1"
                     /translation="MEESKELNTVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(35180..37159)
                     /gene="pknB"
                     /locus_tag="VXM85_000193"
     CDS             complement(35180..37159)
                     /gene="pknB"
                     /locus_tag="VXM85_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1344833.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKTPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPIQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(37156..37896)
                     /locus_tag="VXM85_000194"
     CDS             complement(37156..37896)
                     /locus_tag="VXM85_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1344834.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(37911..39224)
                     /gene="rsmB"
                     /locus_tag="VXM85_000195"
     CDS             complement(37911..39224)
                     /gene="rsmB"
                     /locus_tag="VXM85_000195"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1344835.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(39217..40152)
                     /locus_tag="VXM85_000196"
     CDS             complement(39217..40152)
                     /locus_tag="VXM85_000196"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1344836.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGCDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPKEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(40165..42561)
                     /locus_tag="VXM85_000197"
     CDS             complement(40165..42561)
                     /locus_tag="VXM85_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1344837.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYRHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(42627..42941)
                     /locus_tag="VXM85_000198"
     CDS             complement(42627..42941)
                     /locus_tag="VXM85_000198"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1344838.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(42966..43592)
                     /gene="gmk"
                     /locus_tag="VXM85_000199"
     CDS             complement(42966..43592)
                     /gene="gmk"
                     /locus_tag="VXM85_000199"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1344839.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(43722..45335)
                     /locus_tag="VXM85_000200"
     CDS             complement(43722..45335)
                     /locus_tag="VXM85_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1344840.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(45604..45834)
                     /locus_tag="VXM85_000201"
     CDS             complement(45604..45834)
                     /locus_tag="VXM85_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1344841.1"
                     /translation="MEGVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMDKIRAGH"
     gene            complement(46072..47169)
                     /locus_tag="VXM85_000202"
     CDS             complement(46072..47169)
                     /locus_tag="VXM85_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1344842.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQSMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(47179..47925)
                     /locus_tag="VXM85_000203"
     CDS             complement(47179..47925)
                     /locus_tag="VXM85_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1344843.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKEKKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(48028..48381)
                     /gene="rsfS"
                     /locus_tag="VXM85_000204"
     CDS             complement(48028..48381)
                     /gene="rsfS"
                     /locus_tag="VXM85_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1344844.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(48394..48894)
                     /locus_tag="VXM85_000205"
     CDS             complement(48394..48894)
                     /locus_tag="VXM85_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1344845.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(48895..49488)
                     /gene="yqeK"
                     /locus_tag="VXM85_000206"
     CDS             complement(48895..49488)
                     /gene="yqeK"
                     /locus_tag="VXM85_000206"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1344846.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(49488..50117)
                     /locus_tag="VXM85_000207"
     CDS             complement(49488..50117)
                     /locus_tag="VXM85_000207"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1344847.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVAYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(50169..50480)
                     /gene="yhbY"
                     /locus_tag="VXM85_000208"
     CDS             complement(50169..50480)
                     /gene="yhbY"
                     /locus_tag="VXM85_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1344848.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(50694..51800)
                     /gene="yqeH"
                     /locus_tag="VXM85_000209"
     CDS             complement(50694..51800)
                     /gene="yqeH"
                     /locus_tag="VXM85_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1344849.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPSLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(51803..52393)
                     /locus_tag="VXM85_000210"
     CDS             complement(51803..52393)
                     /locus_tag="VXM85_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1344850.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(52345..53253)
                     /locus_tag="VXM85_000211"
     CDS             complement(52345..53253)
                     /locus_tag="VXM85_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1344851.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(53310..53870)
                     /locus_tag="VXM85_000212"
     CDS             complement(53310..53870)
                     /locus_tag="VXM85_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEV1344852.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQVLHFVPNRNITVVSNFSRTD"
     gene            54009..55220
                     /gene="sstT"
                     /locus_tag="VXM85_000213"
                     /pseudo
     CDS             54009..55220
                     /gene="sstT"
                     /locus_tag="VXM85_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            55288..56241
                     /locus_tag="VXM85_000214"
     CDS             55288..56241
                     /locus_tag="VXM85_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1344853.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            56384..56764
                     /locus_tag="VXM85_000215"
     CDS             56384..56764
                     /locus_tag="VXM85_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1344854.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            56863..57084
                     /locus_tag="VXM85_000216"
     CDS             56863..57084
                     /locus_tag="VXM85_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1344855.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            57101..57415
                     /gene="trxA"
                     /locus_tag="VXM85_000217"
     CDS             57101..57415
                     /gene="trxA"
                     /locus_tag="VXM85_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1344856.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            57720..58253
                     /gene="queF"
                     /locus_tag="VXM85_000218"
     CDS             57720..58253
                     /gene="queF"
                     /locus_tag="VXM85_000218"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1344857.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQINREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISINPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            58250..58339
                     /locus_tag="VXM85_000219"
                     /pseudo
     CDS             58250..58339
                     /locus_tag="VXM85_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(58463..58645)
                     /locus_tag="VXM85_000220"
                     /pseudo
     CDS             complement(58463..58645)
                     /locus_tag="VXM85_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
     gene            58633..58905
                     /locus_tag="VXM85_000221"
                     /pseudo
     CDS             58633..58905
                     /locus_tag="VXM85_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(58965..60761)
                     /gene="pepF"
                     /locus_tag="VXM85_000222"
     CDS             complement(58965..60761)
                     /gene="pepF"
                     /locus_tag="VXM85_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1344858.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETA
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(60772..61515)
                     /locus_tag="VXM85_000223"
     CDS             complement(60772..61515)
                     /locus_tag="VXM85_000223"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1344859.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(61517..62467)
                     /locus_tag="VXM85_000224"
     CDS             complement(61517..62467)
                     /locus_tag="VXM85_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1344860.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     IFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVVAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            complement(62480..>63310)
                     /locus_tag="VXM85_000225"
     CDS             complement(62480..>63310)
                     /locus_tag="VXM85_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1344861.1"
                     /translation="FELSIGKLTKDNDLISSSIEIKEKFIDDFSEIVDDLREYLRIQQ
                     EKNLEKGNAIDFISTLDNMRTSLPVINQPAIDKKYNENLYQHDIVNILTLNYTNVIDK
                     LYNESAKSFSNQLRTNNYKFHIAPPIHAHGTVDICTVLGVSDETQISNSFDEEQKESL
                     IKNLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDGYIWNQIAEQSIRSSVPVII
                     YHYVPHFDAGNPTRVKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIFNLMER
                     "
BASE COUNT        17934 a        14444 c        12226 g        18706 t
ORIGIN      
        1 gtcctgttct tgtttcattt tactatattt ccatgataaa acgcatagta tcaagttttc
       61 ttaatcccct gatactatgc gtgtttgtaa tttttaagat tttgtgctta gagtcgactc
      121 cttattttag atatttaaaa ggaatctcac ttccacagag ccagttgtag acttggtcat
      181 tgacaaatac attcatggtt tcgtgagcat actcaggcat gatacgatag gttttatcgc
      241 aggtcagacg attataaatc gcaaactggg taatgggata gcaaacatcg tcgtccaagc
      301 ccgtaatcat cttaacctca ccttggatac gatgggcaag atttttgaca tcgatatagg
      361 caagggtcgc catgatttcc tcctctgttt catggaaggg gtcgtgaaac ttgaaataac
      421 ggaaaagttc gtcgtaagcc tcgctagtat taccaatctc aatcacccgt ctgaagtctg
      481 acaagaaggg ataaatggca actgttttct gaattcgagg attgagcgct gctgcaacta
      541 gagctagagc ccctccttgt gaggcaccat agctagaaag acgcttctca tcaacctgag
      601 acagactagc aacaatttcg accaactggt aaatatccag ataaacatcc ttataaaaga
      661 ggtggtcccg accttccata gcaccacgga taatatgccc cttcacggta tttcctaaag
      721 gagaacgcaa gccgtcttgt gagtaacctg actgaccccg cacatccatg gaaacaacac
      781 cgtaaccagc tacggtgaag cccagcatgt cggcccagtc ccagccacgt cccatataac
      841 catgaaaatg gaagattagt ggaaccttct cctcactctt tggaagaaca atgcgtgcat
      901 agacctttcc ttccttgctt ccttcaaatg ttaactcata gcacttgact tgaggaatgt
      961 ggaaatctct ttcctccaag tggtaggatg gaagcgtgga aacatttttc acttccccat
     1021 cccagaaagc atcaaagtct tccggaacct catcccttcc tctataggtc ttaatttctt
     1081 ctagcaaagc tggatttttc atagcatgct ccttttattt tgtaatcgtt accacaactg
     1141 tagcatatct atgtaatcaa tgcaagaaag aaggataaat agaaagtgaa tttagattct
     1201 ttacttttag gatgatgggc tgaaagtagt tttagaaacg aaaaagagca tttcgctctc
     1261 tcttctgttt atactagttt tttgcttcct tcttttggaa agtggtttgg taatttactt
     1321 taatcgttac tgtcatatga gagtcctcgt ttctttttga tgactttagt ataagagcca
     1381 aacctaaaaa ctagcttaag ttttccttag agaaagctta atccagatgt tctttctttt
     1441 ccagatgaag ggcaatcatg cgccactctg gatcttcttg ccaagcttct atagaactaa
     1501 tgtagctagc aacctttctt gcttcttcta aaatcggaaa atcttcgata atatcagcca
     1561 cttggaactc tggaagtcct gactgtctgg ttccaaaaat ctcaccagaa ccacgcattt
     1621 tcaaatcttc ctccgcaagg acaaatccat tggtcgtttc tgtcatgatg cgcatgcggt
     1681 ctttcccaga atccgtcttg ggattggcaa cgagaacagc gtaggactgc ttgtcccccc
     1741 gaccgacacg acctctaagc tggtgaagtt gactgagacc gaagcgatcg gcatccatga
     1801 taatcatgac agtcgcattg ggaacgttga ccccaacctc aataaccgtc gtcgaaacca
     1861 gaatatccgt ctttctctcc ttgaaatcct gcatgatctg gtctttttcg tcactcttca
     1921 tcctaccatg tagaagagcc acctctgcct tgcctgcaaa atgagtcgtc aactcctctg
     1981 ataaggcaat ggcatttttc aaatctagag cttctgattc ttcaatcaaa ggagagatga
     2041 cataggcttg ggaacctttt tgaatttccc cctctaacca agtcaagacc tgaggtagtt
     2101 gctcatgttt gatccagcgc gtcacaatag gcttccgacc tgctggcatc tggtcgataa
     2161 tggaaacatc catatctcca aaggctgtga tggcaagcgt ccgtggaatg ggagtcgccg
     2221 tcatcatgag gacatctgga ttgtcacctt tttcccgtaa aatacgcctt tgccctacac
     2281 caaaacggtg ctgctcatcg ataataatca aaccaagacg agcatactcc accccatctt
     2341 gtatcagagc gtgagttcct ataatcaaat cagcctcacc cttggcaatg gtctccaaga
     2401 cttctctctt ttctgcagct ttcaaggaac ctgtcaagag agccagtttc aaattgggaa
     2461 aaaggttctg taaactctca aagtgttgct ctgcgaggat ttctgttggt accattaggg
     2521 cagcctgata acctgctgtc actgccgcaa acatggccaa gccagcgact accgtttttc
     2581 cactccccac atctccttgt aggagacgat tcatgtggtg gtcggacttc atatcagtta
     2641 aaatttcctg caaactcttt tcctgagctt gggtcagggc aaaaggaaga cttgctttaa
     2701 ctgctgtcac tttttcctga gaccaatcca gaaccagacc acttccctga actctatttt
     2761 cagacttgag cgtctgcagc tgcatttgga aataaaagag ttcctcaaac ttgatacggc
     2821 gaagagcctg cttgtattct gccaaatcct ttggaaaatg catagctcgg actgcctgac
     2881 aacgggacat gagtttgtat ttgtctagta aagactgggg cagattttct tctatcaaga
     2941 ggtccagtcc ctgatcaaaa gccgtcttga tgaccttgac cagactggcc tgactgattc
     3001 cctgagccag acgatagaca ggctggaggt catcttctac ctgagccaga accttcatcc
     3061 cagtcagact agccttagcg cggtcccatt ttccaaagac agcaagggtt gctcccaact
     3121 ctattttatc agccagatag ggctggttaa agaaattcac cgcaaaaacg acctctccct
     3181 gcttgagact aaaacgcagg cgattgcgct tgaaaccata atactggaca ctagcaggag
     3241 tcactacctg accagaaaga actgccttct caccgtcttc cagctccagc acctgcttgg
     3301 ttttgaagtc ttcataacgg aaaggaaagt agagcaagag atcttgcaag ttttcaattc
     3361 ctagtttggc gtatttttct gctgactttg gtcccacacc aggcaagaca tgcaagggtt
     3421 gatgtagatt catgctccac tcctttcttt tctaataata ttctctcgga atacggtcgc
     3481 tgaggaggca aaccacctca tagttaatgg ttacgcggta ggtcgctacc tgagttgcag
     3541 tgatttcctt atccccattg gagccaatca aggttacctt ggttccgagt gggtaaggct
     3601 taggcaatcg aatagtgatt tggtccatcg aaaccctgcc gacaattggg caagcttggc
     3661 catctaccaa gacagagaaa ttttgcatgt ctcttgtcca tccatctgca tacccgattg
     3721 gcacggtcgc gatgacttgc tcgctatccg cttgataagt tgctccatag cccatgcaag
     3781 caccagctgg aactgtcttg atatgaacca gagcagactc caaggtcaag gccggtatca
     3841 aatcataagg caaatccaag accgctccac ttggattgag gccatacatg gcatctccca
     3901 tacgaaccgc attgaaaata gtctctacat gccaaagagt cgttgcagaa ttgctagcat
     3961 gaaccagctc tggaacttcc ttcatactag ctaaaatagt tttaaaccgt tctaactggg
     4021 cattaaaata gtcatctgat tcctcatcag cagtagcaaa gtgggtaaag attccttcaa
     4081 cacgaacacc gtgttgttgg agcaaatctt gagcctgctc aacctcactg gcctctctaa
     4141 aaccaatccg tcccatccct gaatcaatct tgaggtggac tgtcaatcca gttaggtcca
     4201 cttccttatc taagagtgct tgaatccact ccagtccagc cactgtcaag gtgaagtcat
     4261 attctttagc tagagcaaca gcttcgattt cagaaactcc taaaatgagg attggcttgc
     4321 tgagtccagc ttgtctgagt tcaatggctt catcgatatt ggaaacgcaa aagccattaa
     4381 catcatcttg aattgccttg gcaacggcaa cagctccatg accataagca ttggccttga
     4441 ccacagccaa cttgagcgtt ccttgaggga tatgagcccc catttgctga atattttgtc
     4501 gaatagctcc cagatgaatc agagccttgg ttggtctatg tggactagct ttcatgattt
     4561 tcctccaaaa tgacactggc tgtcacaaac tgatcggtgt ggctgataga cagccaaatc
     4621 tttcctgaaa atggtgcctg actaaaataa ggcgccccac gttcattgtt caagacttcc
     4681 aaatcctgaa aaccgagctt gctaatgccc gttcccatag ccttggaaaa ggcctcctta
     4741 gccgaccagc gaccagctaa atattctatt tgcctgcgtc ctttgagact ggtgaagcgc
     4801 tccatttcct gagcggtcag tacacgctta gcaaatcctt catgtcgtgt aactgcgctt
     4861 tctatcgaag ccaattcttc gatgtcaatt ccgtgtccaa ctatcattct catcaaaagg
     4921 agttgagatt ccccaactcc atccttttca cttattttgt caaggtaaca taaatctctt
     4981 ctaccaaggc ctctgtattt tcccaaccta ggcaagggtc agtaatagag caaccaaaga
     5041 tctctggttg gttttgacga ccatctgcta ggtaagattc aatcataaat cctcgaaccg
     5101 tctttttaat tttctcattc caatcacgat tctgcaaggt ctggcgaaca attcgaatct
     5161 gctccatata ttgcttgcct gagttatcat ggttggtgtc aatgaggata aaaggatttt
     5221 caagtcccat ggtttcatag cgttcaatgg cttggagtag attttcatag tagtaattcg
     5281 gcatataatt gccatactcg ttgactgctc cacggaggat aacatgggcc aaaggattac
     5341 ctgatgtctc aacttcctgc ccatgataaa ggaaggtttg cttgttttga gcagcataga
     5401 tggcgttaaa cataacaccc aaatttcctg aggttggatt tttcatccct actggtgcat
     5461 caatcccaga agccacaaag cggtgctctt ggtcttccac agaacgagct ccaacggcat
     5521 ggtagctgac caagtcatcc accaagatca gatttgacgg ataaagcatc tcatctgccg
     5581 ttgtcaaacc agtctctgta atcacgcggt agtgcaactg gcgcacagcc tgcaagccat
     5641 taatcaggct tggagcctta gaagtatctg gctggtgaac caatccttta tagccgtctc
     5701 cattggtacg aggcttagca gtatacacgc gcatgaccat gaaaatctta tccgctacct
     5761 tcttttgcaa ggcagataaa cggcgagcat attccaagac cgcctcttca ttatcagaag
     5821 agcaaggacc aatcaccaag agaatacggt catcttcccc tgaaataata tctgccaatt
     5881 ccctgtcacg gctttccttg agtctcaagg cttccgcaga caattgggtt tcagccttga
     5941 tgacttccat atcgatttct tgaccttttt caataaatgc catcttattc tcctaacgtt
     6001 ttgtaaattt ctctgacaag agcttctgtg ttatcccaac ccaggcaagg gtctgtgata
     6061 gacttaccaa atacttctgg ctcattttgt cgaccatctt ccagataaga ctcaatcata
     6121 aaaccacgaa cgaactgctt aattttttca ttccaagcac ggttaatcaa ggtctggcgg
     6181 acaattcgga tctgttcaat atactgctta ccagaattgt catgattggt atcaatgatg
     6241 ataaaaggat tttccaagcc cattttctca tactgggcaa tggtatcaat taaattgtca
     6301 taatagtagt tgggaatatt ttttccatac tcattaagag caccacgaag aatagcgtgt
     6361 gaaagcgggt tcccagttgt ttctacttct tttcctaaga aaaggaaact ttgtttgttt
     6421 tgagcagcat aaatcccatt aaacatgaca ttgagatttc cagaggttgg atttttaaaa
     6481 ccagtagaaa atcctgcccc acttgccaca aagcggtgtt gctggtcttc aactgaacgg
     6541 gcaccaactg ccatgtaaga aatcaaatca tctacaagcg gaaggttttc aggataaagc
     6601 atttcatcag cagttgtcat ccctgtttct gtgatgacac gatagtgaag atggcgaacg
     6661 gctttgattc cattgataag actaggcgct tctgtcgcgt taggctggtg aatcaagccc
     6721 ttatagccat ctccgttggt acggggtttg gcagtataaa cacgcataac cataaagata
     6781 cgatctgcca cttcttcttg taggactgcc aaacgcttag cgtattcaag gacagcttct
     6841 tcgttgtcag atgagcatgg cccgattacc aagagaattc gctggtcttc tccacgtata
     6901 atggcttcta gctcttgatc tcgctgtgat ttcctctcca aagcctgacc ttctaatttt
     6961 gacaaggcac gaacttcttc tatatttatt ttagagcttt ttgctgtaaa taccataact
     7021 catctcctta taaagcaaac ggatacccag taaaaattta cttttcacaa gatatccgcc
     7081 tctaaactat ctcattttat tgtcttttac cgtgacagtt tttaaatttc ttaccagaac
     7141 cacatgggca aagttcattg cgtccaatct ggctcaaatc caaatcttct ggcatacttg
     7201 cttggtgagc agcgatattg cgagtcgctg ttgtactgat atgacgttct gcctgtggtc
     7261 tttcttgttc atgaatttgt gctttcatca tcaagcgtgt cacatcaaac tcaatcgaac
     7321 caatcatatc attaaacata cggaaacctt ctgcctgata ctcaacaaca gggttgttct
     7381 gagcatagcc acgaagtcca accgcgttac gcaattgatc aagggcatcg atatgatctg
     7441 tccacttgtt atccaccact cgtagaatca aaactttttg gaattcttta actgcttctt
     7501 catcgcgtag ttttgaaacc tgactatcgt aaaccttcaa ggcacgttgg aaaagctctt
     7561 ccttgatggc cttatcagac aagcctgaca agtcttccat cgtaatagaa tcttcaggaa
     7621 gcaagttgta cttagcgaag ttcaaaattg cctctagttt ctcatcttgt ttagcacgcg
     7681 catgaccgtc aacgacacga ccaatcgtgc gtttgatcat tgcctgaatt tcaggtgcca
     7741 agtcacggtc tgcagtaatg acatcataac gttgagtata gataatctca cgttgttcac
     7801 gcatgacatc gtcgtattga aggacttgtt tacgggtatc gtagttattt ccttcgacac
     7861 gtttctgagc tgcttcaacc tgacgcgtca acatgcgaga ctcaatggcc tcttcagaca
     7921 tgttcaagcg ttcaaagatt cccttcaagc gttcagaacc aaaacgtttc atcaaatcat
     7981 cttcaagaga taggtagaat tgtgactcac ctggatctcc ttgacgacct gaacgtccac
     8041 gaagctggtt atcgatacga cgactttcat gacgttctgt accaataaca caaagtcctc
     8101 caagttcacg aacaccttca ccaagcttga tgtcggtacc acgacccgcc atgttggttg
     8161 cgatggtaac ggcaccacgt tgaccagcat tcatgatgat ttgggcttct ctatagtggt
     8221 ttttggcatt caagacttcg tgaggaacac cagctgcaac caatttctta gaaatgtagt
     8281 cactagtttc aaccgctact gtaccaacca agacaggttg acccttttgg taacgagcct
     8341 taacgtcttc gacaaccgct ttaaacttag attcgatact tgcataaaga aggtctgagt
     8401 ggtcaatacg ttgaacagga cggtttgttg ggattggaat aacacgaatg ttgtagattt
     8461 cacgaaattc ttcttcctca gtcttacctg tacccgtcat accagacaat tttttgtaca
     8521 tacggaaaag gttttggtac gtgattgagg cagatgtctt ggtttcatcc tggattggca
     8581 caccttcttt ggcttcaata gcttggtgca atccatcaga ataacgacga ccttccatgg
     8641 tacgacctgt aaattggtcg acaatcaaga tttcttgctc ttcgctcacc acatagtcaa
     8701 tatcgagaag catgatgtag ttggcacgaa gggcgttatc gataaagtgg gtcaaagcca
     8761 cgttttcgat gtcatagagg ttttcaagtt tgaagtagct ttcagccctg tcaatccctg
     8821 aatcagacaa accaatagtc ttagactgca catcgatgat gtagtcatct ttgttcaaag
     8881 attttacata gtggtctgcc atgtgataca actgactggt ttcaaccgca ttggcacctg
     8941 atacaatcaa aggtgtacga gcctcgtcaa tcaagataga gtcaacctca tcgaccaagg
     9001 catagttaag cggacgttgt accatgtttt cagcgcgaac gaccatgttg tcacgaaggt
     9061 agtcaaatcc gatttctgag ttagttgagt aagtaatatc acactcatag gcttcttttt
     9121 tctccattgg agatttggta gccaagttaa tccctactga caaaccaagc caagagtaca
     9181 attcacccat ctcagtcgcg tcacgttctg acaggtattc attaaccgta actacgtgaa
     9241 cccctttacc tgaaagggca ttgaggtata ccggcatggt cgcagtcaag gttttccctt
     9301 cccctgtacg catctctggc acgtcaccat ggtgaagaac aatccccccc atgacctgaa
     9361 ccttataagg gaagagacct aggacacgtt tggcaccttc acggacaacc gcaaatgctt
     9421 cgtaaagcaa tgaatccagt gattctccat tttgataacg ttccttaaat tcaactgttt
     9481 ttgcttttag ttggtcgtca gtcaaagcag ccatttggtc ttcgtatttg aaaaccttgt
     9541 cagccatctt ttccagacga cggatttctc ctttatcatt ttcgataatt gtttttaaaa
     9601 tattagccat gtttttcctt actttaaatt ccgaatattt tagaatgttc ctttaatttt
     9661 agcacaatta ctgattattt tcaaggaaga atccccattt tgaaaaggaa aaagaggtta
     9721 gttttcaagc taacctctcc gacttttatg actgtttaat tgccaaaaat tggcaaaatg
     9781 gcaaatgtga taaataagtt aatccaaaga gaaagacagc aaatcagtcc aaaaacaaag
     9841 agactgactt tcttggacag caaggccatc aaaatggaca gaattccaaa aactagcaag
     9901 actttctgta tgatgaagat gtcaatcctt acccccagaa tcgctctatc ccaagggata
     9961 aagaagaaag acagccagat cagcagaact aaaccaatat aaaccttgga atagcgtgta
    10021 ataaatgatt ttagatgtgc cataagccat ctcctaggat tgtatatcct ttacatattg
    10081 tatatgtcca ttttcgacaa ctaatacatc tgtacaaaga tctgaaatat cttccgatat
    10141 gtgcgacgtc aggataacca gaccattttc ttttttcagg taagataaaa tgacctgttt
    10201 ggttaacctc acacttttct catccaaagc attcataggt tcatcgagaa agagtataga
    10261 aggagaggaa ataaaggctt gaatcaaggc cattttttgc tttgttccca aggataaatg
    10321 acggtattca acgtcttcaa attcctgtaa atcatagtat tgaatccaat aggcaattct
    10381 tttttccgta actttagatg aaaaatacct taacagttcc aaattttctc tcagggataa
    10441 atgagataaa aactcgactt tttcaattaa aattcctgca tcctgaatat aatgattttt
    10501 taccccgtaa actttaccat cttgaagaac ttttcctttg tcaagcttaa tataaccagc
    10561 aagtatcttt aaaagaaccg tcttgccaga tccattatca cctttaagtc cataaatttt
    10621 tcctgattga aacaccaaat tcaactgatt taaaataggt cttttcccat aaattttctg
    10681 tccatttata atttcaagtc tcatcgttta tcctttctta aatttctctt ccaagcgact
    10741 aagaatcaac ataactaata agcttaagag tactaaatac actgttacaa tatgatccat
    10801 catccattct tgtcccacaa atgccaaaaa atagagaaaa aaagaaaaac cagcgcccgc
    10861 actaatcagt gctatcagat aagacagtag taagtacaac atcattaact gagcaagtaa
    10921 aaagaaaagt gcgactgttt tgaaatgaat tcctagcgga agtaaaccaa gaaaccagac
    10981 aaacaaatct tgagtaaaaa atttagaaat gacaaccaat cgtttccttg tataatcgaa
    11041 aaaggagctt gattgatagc gaatcaagtg atggtaatgg tctgccaaat cactttcaag
    11101 taaaatgaca gagagccatc ctaccatgaa aagcgaaagg tttaacaaga gtttggggat
    11161 ttctaaattc gcttcagaat agtcattata ggaaaaaatt gaaatcaatt ctctcttgaa
    11221 aaagataaca gcatcctgct tttgaaccgc tgttgacaat agatagttta tcaaaatcat
    11281 catcagaact atacgaagta gtaaaacgat ctttctcttt agccaaatat tgttaaaatt
    11341 cttaaacatt atcttcatat tttttaagta ccatataagg gacaacataa aggatataag
    11401 gagtaaagag tttcatcaaa cttacatccc catagctagc ttgcatcaaa ctcgtcatag
    11461 gaaccatata gtaaggcaag gcactataga gcagcataga accaagccaa agaaacatca
    11521 aataggtgat tgccgaacga gtcacattcc ccacataatc aactatttgt aaaaaataga
    11581 ttgcattgat gaaaataccg attaatagga cacaagtaaa gaacaaatag ctttttatct
    11641 ctccatctag gagtctttgt aaaccactcc catcagaaaa tagagaattc cattcaagag
    11701 gagaaaaagt cccaaagaaa taggttataa ttgcaattat cagcacagtc actaaatata
    11761 tcaaacaagg gatacttgca acttgcaaag tcaactttct ttttaaccct tgatagctca
    11821 cttctcttcc aatactcaat cgtaaaactt tatttttcag ctcaatatat tgaaaaccta
    11881 gcaaaacaat gatgattgga ataataaatg attgataaaa acctagattg aactggagaa
    11941 tacgaaaatc taatgctgat tttaataagt catttttaat tgcttgtcca tcgaatttag
    12001 ctagatcggg cgcatgaata tcaaaaaaga aataagtcat caaaacgtca cgataaacca
    12061 aagcaagaaa aagcaaagta agcaagacat aaacaaattt atgccttgcc aaagttaaga
    12121 gaggaatctt cataagccac ctcctacaaa tagaaagtaa tataatactc aatgaaaatc
    12181 aaagagcaaa ctaggaaact agccgcaggt tgctcaaagc actgctttga ggttgtagat
    12241 aagactgacg aagtcagtta catatataat ccaaggcgac gttgacgtgg tttgaagaga
    12301 ttttcgaaga gtataaatca atgccttccg cctttagact tccctagttc ccgtctcaag
    12361 tcaagcattt ttttgaaaaa gacataggct aaccaatcac tagcaaaagc gataagtagt
    12421 aataatagcc aaatacccca taacttgata tctgtcaaat ttctcaagac gatattgaaa
    12481 aacagaactg aaaaaactgt ccaagcaagg ctaaaaagag catagagggt gatgtaaatc
    12541 cagtaaaaat agtaaaaaat tgggaaaaat ttactttctc tattatcctt gtcaatccag
    12601 ctgaaaaagt aaaaacaggg taataagatt atcattttaa acaagtgttc catcacaaac
    12661 ctcctctttt aaattgtttt ctttagtttc gttctattaa atgttattac caatataaac
    12721 cttggaatag cgtgtaataa atgattttag aggtaccata aggccacctc ctacaaatag
    12781 aaaccgatat aaatcaatgc cttccaccct tagacttccc tagttcctgt ctcaagcgaa
    12841 acatttcttt gaaacaggaa taagttaacc aattcatacc aatagctagc agaataaaaa
    12901 gaaaccaaat gccccataac ttgatatctg tcacatttct caagacggta ttgaaaaaca
    12961 gaactgaaac aactgtccaa gcaaggctaa aaagagaata gaaggggatg taaaaccagt
    13021 aaaaataata aaaaattgga aaaaacttac tatttctgtt ggccttctca atccagttat
    13081 caaaataaaa gtacggtgct aaaagtaaga atttaaacaa atgttccatc accgacatca
    13141 ccctctttct tttgatagcg ttttctatta ttttattata tcaaaaaaat ccggaactgt
    13201 cattccagat tctacttttt tatttgcgtt ttcttgcgat gagatgaatc ggtgttccct
    13261 caaaaacaaa ggccttgcgg atttgatttt ccaagaaacg caggtaagaa aagtgcatga
    13321 gttcttcttc attgacaaag atgacaaagg ttggtggttt ggttgccact tgggtcgcat
    13381 agaaaatctt gagacgtttt cctttgtctg tcggtgttgg gttgatggca atggcatcca
    13441 tgatgacatc gttcaagaca gctgatggaa tacgtgtatt ttgactttcg ctgatttgct
    13501 taatcatctc aggaagtttg tggagacgtt gcttggtcaa agcggataca aagataatcg
    13561 gtgcgtaagg caggtattgg aactgctcac ggatatcttc ttcccagttt ttcatagtgt
    13621 ggttatcttt ttcaagcgta tcccacttgt tgaccacgat aatcatccct ttaccagctt
    13681 catgggcaaa tcctgcgata cgcttgtcgt actcacgaat gccttcttcc gcattgatga
    13741 ccatcaagac cacatctgaa cggtcaatag cacgcatggc acgcataaca gagtatttct
    13801 cagtattttc ataaacctta ccagacttac gcataccagc cgtatcaatc atggtaaact
    13861 cttgaccatc tgtatctgta aagtgggtat caatggcatc acgagttgtt ccagcaacag
    13921 gactagcaat aacacggtct tcgcccaaga tagcattgat caagcttgat tttccaacgt
    13981 ttggacgacc aatcaggcta aacttaatga catctggatt ttcttcttca tattcatttg
    14041 gaaggttttc cacaatcgca tctagtacat cccctgtacc gataccatgg acagatgaga
    14101 taggcagtgg ttcacccaaa ccgagagcat agaaatcata tatatcattt cgcatctcag
    14161 ggttgtctac cttgttaact gcgaggataa ctggtttgtg ggtcttataa agcttacggg
    14221 ctacgtattc gtctgcatcg gtaattcctt ccttaccgga cacgacaaag acgataacat
    14281 ctgcttcttc catggcaatt tctgcctggt gcttgatttg ttccatgaaa ggagcatcga
    14341 catcatcaat tcctcctgta tcaatcatgc taaaagaacg attgagccac tcacccgttg
    14401 cataaatacg gtcacgtgtc actccttcga catcttctac aatggagatt cgctcaccag
    14461 cgatccgatt aaatagggtt gatttcccaa cattgggacg tcctacaatg gcaatagttg
    14521 gtagggccat aatttctcac tttctacaat aatttcttct gttcaagatt ttttctagtt
    14581 gagcttggtt cagcttgacc aaactgttct gctaggcgct gactccagct tgtggtcgca
    14641 cgcgccccag catagtcagc ctgaacacgg tcataagctt ggattgcctc agttgactgt
    14701 tcttggtatt cttcctcaaa gacaacattc tctagtggca gtctcggttt catatcatga
    14761 tgttgatttg gcacacccag tgccatccca aagacagaat aggtgtagtc aggtaggtta
    14821 aagagctctg ccacttcttc agacttgtat cgaaccaaac cgataatcac accaccatag
    14881 cccaagcttt cagctgccaa caaggcgttt tgtccagcaa gagctgcatc gaccgaacta
    14941 atcaagagac cttccacacc ttggggttgg aaggtgtcgg tatgaagtcg ggctcccttt
    15001 tctgctcggt tcaaatctcc gacaaagaga aggaaaacag cagactggcg aatggcttct
    15061 tgaggtacca attcatacaa ggcatctttc ttctcttgac ttcgtaccac aatcacagag
    15121 taggattgga aattcttcca agatgatgcc atctgggctg ctgtcaaaat ctcatttaag
    15181 tctacttggg gaatttcttg ctctttaaac ctgcgcactg aagtatgagc cttcatcaat
    15241 ttaatggttt ctgtcatcga cggtttactc cttctaaacg agtctcctca gccaaataac
    15301 ggatgcgttc catgacccgt ctggcttccc aggtttcgtc atttccatgt ttcactttcg
    15361 caaaatgctt ctccaaatct tcaaagttga agttggatgt gaaaaaggtc ggtaaatttt
    15421 cctgcatccg atattggaga atgacctgca ggatttcgtc acgcacccaa acggttgatt
    15481 gctcggcgcc aatatcatct aaaatcagga catcagacag cttaatctca tccaccaagg
    15541 tcttaacatt gccatcactg atagcatttt tgacatcaat gacaaagcta ggatagtgga
    15601 ggagagtgga tgatacacca cgtttttctg ataaatcatg agctagggca gccaccatga
    15661 aacttttacc cacaccaaag tctccataca agtaaagacc tttcaaatta ttggggtaat
    15721 cgttaacaaa ttctatcatt ctttttagaa cagttaagcg ctgaacatca tcacgataaa
    15781 catctaaaaa actgacattt ttcaggctag atggaaaatt aattaagttg agacgtttct
    15841 tgatagccgc ttctttttca gctgcgatta gttcaggagt ttcttcataa gaaacatccg
    15901 catagccatg gttcatgact aatataggct tgtagccttt ggcaatataa tctgtatctc
    15961 ctcggaggaa tttatctctc tcagtgatgt attggttaaa cttggaaata ctacggttca
    16021 actcgtcctg attgagggat tcttgctgga caaaggccgc aacatcagga tccttcatga
    16081 ttttctgaac taaatcttga tagtgaaaac gacttggttg acgtttgagt acatctccga
    16141 cactttccat ctaatcacct ccgttatcta attttgctaa taattctagg gtttggcgtt
    16201 ccatctcttc taattcctca gcggatgacg tatttttata ttcaggatta ctccatttag
    16261 gaatattggt ttttgctggg gcagtctgat tctgtttttg tgtttttgct ttctgttctc
    16321 gctcacggat gcgcaagact gccttttctg ccgaatgaat cttttgatag gcatagtcat
    16381 tggctacctt catggcatat ttctcattga tatttgccga atcgacctta ttaaaggtca
    16441 ataaaagaat gatattgatg acttcgtcca gcaagcccaa gccagccatc tgttgcaaga
    16501 gttctctttc tgtttgggta atggttccct tgcgtgtttg cttgatttct gccaagaact
    16561 gcagggcagt tttacttttg gcttctttga taatggttgt ttccttaaga ctaaagtcag
    16621 aggaaactgg tttttgagca attttttcac gcatgcgttt ggttgaaata acctgagaaa
    16681 cagctgttga cttggccaat tgataggttt caaaccaagt ccatttcttc tcctcggcaa
    16741 tagcaaagag gtttaagaca tcggactgct catccgcaaa acgaagtcca tctcgagcca
    16801 tcagctggcg aaaatgttcc aagtcaaaat cattggccac tttcttcttg agaccaaggt
    16861 cttcttgact gcctagttct gccaattctg gaaagacttg attgagtgag acaggtattt
    16921 cttcaccatc agcactttca actttcaaat cctccacagc agagtcccca atctttttct
    16981 ctaagagtct gcgataaaca ggatgcccca agaagtcttg actagataga ggagcatgga
    17041 gggctagctg ataaacatcc cccttttgat agagagtcaa gagattaaaa gcagataaga
    17101 ttttcaatga ttttatcagt ctatccattc caaagttaag atgattgagg atacttgaaa
    17161 aaagatattc ctttctacca ttatcccaaa aactaatcgt ataaagataa aggctcagtg
    17221 cctcctgacc gataatcggg aggtagcact gtaccagaga tgaggtatct tgcgacaccc
    17281 gattattctt tagataagaa aaacggtcaa ttggcttcat ttatctttcc tttttctttt
    17341 tagaggactg ggtgatttgt tggagcaagc tctctaactc actgacatcc ttaaaactac
    17401 gatagacact agcaaaacgt acataggtaa tctcgtccaa ttcagccaac tcctccatga
    17461 cgagtgaacc aatgtcctca ctttgaattt cattttcatt tcgaccacgg agtttctgtt
    17521 cgatacgatt gactaccatg ttgatttcat cacttgacac aggacgtttc tgggctgagc
    17581 ggataatccc attaaagatt ttatctctgg agaattgttc ccgtgtgcca tcttttttaa
    17641 caaccactaa ggttctttct tctactcgtt cgtaggttgt aaaacggtgt tggcattcgt
    17701 cgcactcacg tcttctacga atggtgttcc cttcttctgc ttggcgacta tcgataacac
    17761 ttgacttggt agccccacat tttggacaac gcatcctttc cctccttatc gttttctttt
    17821 cattatacca ttttttaagc gattcccaaa acaattcttc tttttgcttg acaagttttt
    17881 tgttttgttg tattatttaa ttaagacaaa agggtaagag aaaggagacc aagatgtcct
    17941 ggacatttga caacaaaaaa cccatctatt tacagattat ggaaaaaatc aagcttcaga
    18001 ttgtttccca tacactggaa cccaatcaac aacttccaac cgtgagggag ctagctagcg
    18061 aggctggtgt caatcccaat accatccaaa gagccttatc agaccttgaa cgagaaggat
    18121 ttgtctacag caagcgaaca actggacgat ttgtgactaa ggataaggag ctaatcgccc
    18181 agtcacgcaa acaattatca gaagaagaat tggaacactt cgtttcctcc atgacccatt
    18241 ttggctatga aaaagaagaa ctaccaggcg tagtcagtga ttatattaaa ggagtttaag
    18301 cctatgtcat tactagcatt tgaaaatgta tccaaatcat atggagcaac accagccctt
    18361 gaaaatgttt ctcttgacat tccagctgga aaaattgtcg gccttcttgg gccaaacggc
    18421 tcaggaaaaa caaccctgat taaactaatt aatggcctct tacaaccaga tcaaggacgt
    18481 gtcctcatca acgacatgga cccaagccca gcaaccaagg ccgttgtagc ttatttgcct
    18541 gatacgacct atctcaatga gcaaatgaag gtcaaagaag ccctaaccta cttcaagacc
    18601 ttctataaag atttcaatct tgaacgcgcc catcatctac ttgcagacct gggcattgat
    18661 gaaaatagtc gtctcaagaa actatcaaaa ggaaacaaag aaaaggttca actgattttg
    18721 gttatgagcc gtgatgctcg tctctatgtt ttggatgaac ccattggtgg ggtggatcca
    18781 gcagcccgtg cttatatcct caataccatt atcaacaact actcaccaac ttctaccgtt
    18841 ttgatttcta cccacttgat ttctgatatc gagccaatct tggatgaaat tgtcttccta
    18901 aaagacggaa aagtcgtccg tcaaggaaat gtagatgata ttcgctacga gtcaggtgaa
    18961 tccattgacc aactcttccg tcaggaattt aaggcctaag caaaggagat tatttatgtt
    19021 ttggaattta gttcactacg aatttaaaaa tgttaacaag tggtatttag ccctctacgc
    19081 agccgtgcta gtcctttctg ccctcatcgg aatacagaca caaggcttta aaaatctacc
    19141 ttaccaagaa agtcaggcta ctatgctact ttttctagct acagtctttg gtggcttgat
    19201 gcttacactt gcgatttcaa ccattttctt gattattaaa cgcttcaaag gtagtgtcta
    19261 cgaccgacaa ggctatctga ctttgacctt gccagtttct gaacaccata tcatcacagc
    19321 caaactaatc ggtgccttta tctggtcatt gattagcacc gctgtattgg ctctaagtgc
    19381 tgttattatt ctggctttaa cagctccaga atggattcct ctttcttatg tgattacatt
    19441 tgtagaaaca catctccctc agatctttct tacaggtata tccttcctac taaataccat
    19501 ttcaggaatc ctctgcatct acctggctat ttccattgga cagcttttca atgaataccg
    19561 tacagcactc gctgttgcag tctacattgg tatccaaatc gtcattggat ttattgaact
    19621 tttcttcaat cttagttcta atttctatgt caattcactg gtaggactca atgaccattt
    19681 ctatatggga gcaggtatag ccattgttga agaactcata ttcatagcta tcttttatct
    19741 cggaacctac tacatcttga gaaataaggt taatttgctt taaataattt ttacctagat
    19801 atgtaacata ctcatagaac aaaagagacc aggcaaaaag tctttaaaat tagaaaacgc
    19861 atagtatcaa gtgttgaata tgaactgccc cccaaaagtt agacagaaaa aatctaactt
    19921 ttggggggca gttcaaaaaa tccttgatat tatgcgtttt ttgtgagctg acttatttcc
    19981 tttcactata tcgcaaaatg aaataagaac ggaacgatgg gattttggaa ttcaaatcaa
    20041 tttgtaagaa tgttttagaa gtaatattat cctattccag attcagttca ctatacaatt
    20101 gagttttcaa gcaacttgtt tacataatgt gtacataatt aggttcgtga ttccaccctt
    20161 ttcaccttta aaaacctcgc tttcgcaagg ctcttctatt tataagataa ggcacgttta
    20221 aaggttttcc aaatccctaa atcatccgtt tgaagaacga gactagcata catgcttccg
    20281 ataaatcctg ttgctaccac cgcaaaaatc actgtaatag caagtgaaat ccatgcttct
    20341 gctccccccg catagtcatt aatcgttcga aacggcataa agaaggtcga aataaaggga
    20401 atataagaac caatcttcaa gaggagattg tcaccagctg cacctagagc tgtcactcca
    20461 aaaaaaccac ccataatcaa aatcatcaaa ggcgacaagg ctttccctga gtcctcagga
    20521 cgagaaacca tagatcctag gaaggctgcc aagactacgt acatgaaaag actgatcaaa
    20581 ataaagagca aggtattcag tgagatagca tctcccaagt gatccaaaat accagactga
    20641 gccaagaatg gcaaatcttt aaagagcaaa acggcagcca gaccacctac aacatagatc
    20701 ccaatatgcg ttaaaatcac tagaaacaga gccatcatcc gcgcatagaa atagtgactt
    20761 gcccttatgc tagaaaaaac gacttccata attttggtgc ctttttcact ggcaacttcc
    20821 tgagctgtta cacccgcata ggtaatcaga atcatataaa gaaagaatcc taaggcacct
    20881 gctgcaattg tttgaataaa ctttttattt tccttggctt catcaatctt ttctgtgaat
    20941 tgaattgtct gcgctaagcg tttttcctgc tcttgagaca aggaagcagt tgaacgatta
    21001 agctgatttt gcagttcatt gagtgtacct gtaacctcaa atttaattcc attttcaagc
    21061 gatgtttcgc catgataaac tgcctttaga acactatctt cttgatcaat ggtcaaataa
    21121 ccttttaatt tttcttcttt aattgcttct ttggcacttg cttcgtcttt atagtcgaag
    21181 ttaacaccat ttacattctt cagtccttct gctacagatg gcactgttgt cactactgcc
    21241 actttattat ttttagccat agaagaacct tggatatgcc caattcctac agagattcct
    21301 aaaaagagga acggcgaaat caccataaag aagaaactcc atgactcgac atgtcgaaga
    21361 taggtttcct tgattacaac ccacatattt ctcatacttc cactcctgat tctagtttaa
    21421 agatttcatc gatagttggc gcttgttggt caaatgttgc gatatattga ccttgagtca
    21481 agattgggaa gagttccctt ccagcgctct catcctccaa aatcaatttc caactgcctt
    21541 gtttggtcaa gctcacctgt ttgacatgag gaagattttc caattcttcc ttgcttcgtt
    21601 cacttgaaac aaagagacgc gttttcccgt attgattgcg gacatcctga actggtccgt
    21661 gcaagaccac acggccatct cggatcatca gaatatcgtc acaaagttcc tcaacattgg
    21721 tcatgacatg gtcagaaaag ataatggttg ctccgcgctc tttttcctga aaaatgactt
    21781 gtttgagcaa ttctgtatta actgggtcca atccactaaa aggctcatcc aagataatca
    21841 ggtctggttc atgaatcaga gtaataatga gctgaatctt ctgctgattt ccttttgaca
    21901 gactcttgat tttatctgtc agctttcctt tcacttccaa cctcttcatc cattgaggga
    21961 gtttttcttt gacttctttg gcatccatgc cttttagagt cgccaagtag cgaacttgtt
    22021 caagaactgt caatttaggc atgagactgc gttcttcagg cagataacca atccgagcat
    22081 aggtctcctg acgaatatcc tgaccatcca gaccgatttc tccctgatat tctaggaatt
    22141 tcaaaatact atggaaaatc gttgtttttc cagcaccatt ttttccgact agtcccaaaa
    22201 tacgacctgg tcgcgcttga aagtcaatac caaacaaaac ttgcttggag ccaaaacttt
    22261 tctctagact tcttacttct agcatctttc acctccgaaa tttcttgcac tcattatact
    22321 cctttttgat agcctttaca atgtttttgt ccatttttag aagactattg ctgtgtaaaa
    22381 tatggcctgg agcactttta tactcaatga aaagcaaaga gcgaactagg aagctagccg
    22441 taggctgctc aaagtacagc tttgaggttg cagataaaac tgacgaagtc agctcaaaac
    22501 actgttttga ggttgtggat agaactgacg aagtcagtaa ctatatctac ggcaaggcga
    22561 agctaacgtg gtttgaagag attttcgaag agtattagtg ataaatccat tatacagtag
    22621 caaactcaat ttataccttc cgctcctcaa ctgtctattt ttaatcctga attgttattt
    22681 gagtaactcc tttttcctca taaagttttc ttcctctaaa acttctggaa aaaagcgaat
    22741 agtttcagac aacattttta taagaaacaa gttcatctgt catttcaaga aggagtaatc
    22801 ctttatctac taatggacgg aacggaattc aaccgcttgt ccgatatatt ttctaaggat
    22861 tatatagtaa aatgaaataa aaacaggaca aatagatcgg gacagtcaaa tcgatttcta
    22921 acaatgtttt agaagtagat gtatactatt ctagtttcaa tctgctatat ctattatgca
    22981 caccactata ggatctaata aaaatcacaa caagttcatt catagatggt tacctcaggg
    23041 aactaagaaa acgactacaa aggaagtcgc attcatcgaa aagtggatta acaactatcc
    23101 taaaaaatgc ttgaactaca agtcccccag agaagacttc tggatgacta acttgaactt
    23161 gaaatttagc aataattaat tcaccatcta actatattta gtaattattt cagaactgat
    23221 taatattaaa attaactaac aattcaaagg attcatacta gccataaatt acgtccatca
    23281 gagagagact cttactactt ttagatttta gtctttctag cttcagaata catctaaact
    23341 ttagggaaaa tgactattcg aaagcgcgaa tgcctcaaaa ttatctcaga taagctattc
    23401 gaaacttaga atgcttttaa atttatggaa ttgcgattat tcgaaaccta gaatgcatat
    23461 aacctttagt tgacagacct attctaagtc tcgaagggct atttactttc tattccttat
    23521 caaaaaagac tcattccccc tttctcctcc aaaatatggt atagtagaaa tatactatct
    23581 atgaggagtt tacatgtcac aggataaaca aatgaaagct gtttctcccc ttctgcagcg
    23641 agttatcaat atctcatcga ttgtcggtgg ggttgggagt ttgattttct gtatttgggc
    23701 ttatcaggct gggattttac aatccaagga aaccctctct gcctttatcc agcaggcagg
    23761 catctggggt ccacctctct ttatcttttt acagatttta cagactgtcg tccctatcat
    23821 tccaggggcc ttgacctcgg tggctggggt ctttatctac gggcacatca tcgggactat
    23881 ctacaactat atcggcatcg tgattggctg tgccattatc ttttatctag tgcgcctata
    23941 cggagctgcc tttgtccagt ctgtcgtcag caagcgcacc tacgacaagt acatcggctg
    24001 gctagataag ggcaatcgtt ttgaccgctt ctttattttt atgatgattt ggcccattag
    24061 cccagctgac tttctctgta tgctggctgc cctgaccaag atgagtttca agcgctacat
    24121 gaccatcatc attctgacca aaccctttac cctcgtggtt tatacctacg gtttgaccta
    24181 tattattgac tttttctggc aaatgctttg acacgtaaaa aatccgtttg gtttcccaag
    24241 tggattttta aagcgtagat taactatagc ttgatactaa atatactttg gtatggaaat
    24301 tatgcatatt tttcgatagt gaggcgagga cttacctagc ctttccgccg tgatagaaac
    24361 acctgaaatc taatggtttc aggtattcgg aaactttgag acactaggct caaagtttag
    24421 gtatggaatt tcgaagaaag tcgctaccgt ccgtaatcac ttaaggaaag gctcaaaaat
    24481 attgttttca accacaaaat ccgtttggtt tcccaagcgg attttgtgct ttattttgaa
    24541 acttcttttg caagaacaaa gttcccaagt gtggcagaac catttcctgc gactgctggc
    24601 gtcacgatat agtcacgcac atctggtact ggtaggtaac cattaagaag agatgtaaat
    24661 ttctcacgga cacggtccag catatgttgt tgagccatga cccctccacc aaagacaatc
    24721 acgtctgggc ggaaagtcac tgtcgcatta accgcagctt gagcgatata gtaggcttga
    24781 acatcccaaa cagggttgtt gagttcaata ttttccccac gtacacctgt acgagcttcc
    24841 aaacttggac cagctgcata accttctaga catcccttat ggaaaggaca aacaccctta
    24901 aactcttttt caatatccat tgggtgtcta gcaacataat aatgacccat ttcagggtga
    24961 cccacaccac cgataaactc accacgttgg atgacgcctg caccgatacc tgtaccgatt
    25021 gtgtagtaaa ccaagttttc gatacgacca ccagcattgt tacgggcaac catttcaccg
    25081 taagcagagc tgtttacgtc tgttgtgaag tacattggca cgtttagggc gcgacgaagg
    25141 gcaccaagca agtctacatt tgcccagttt ggttttggag tcgtcgtgat aaagccataa
    25201 gtttttgagt ttttgtcaat atcaatcggc ccaaatgaac caactgcaag accagcaagg
    25261 ttatcgaatt ttgagaagaa ctcaatggtt ttatcgattg tttcgattgg agttgttgtt
    25321 ggaaattgtg ttttttctac aacgttaaag ttttcatcac cgacagcaca gacaaacttt
    25381 gtaccgcccg cttccaagct tccatataat tttgtcatga taaacctctt gtttttattt
    25441 tctttattat agcatacttc gaaagtctaa atgtctctat tttttagatt ttcctctgta
    25501 aatcttacta tctaataaaa acgaacaaac atgtcatttg ttcgttttca cattagagag
    25561 gattgattag attttcactt cgatcacagc atcccccttc gaaactgaac ctgttgcgac
    25621 tggagctact gaagcgtagt cagctgtatt tgtaacgata accattgttg tatcatcaag
    25681 tccagctgca gcgattttgt ttgagtcaaa tgttccaaga acatcgccag ctttcacctt
    25741 attaccttga gcaacttttg cttcaaaacc gtcaccgttc atagatacag tgtcaatacc
    25801 aacgtgaatc aaaacttcag caccatttct tgttttcaaa ccaaaagcgt gccctgttgg
    25861 aaaggcaatt gaaacttcag catcagctag tgcatagacc acgccttgac ttggtttcac
    25921 agcgatacct tgtcccatag ctccacttga gaagactggg tcattgacat cagcaagagc
    25981 gacaacatca ccgacgatag gagttacaag tgtttcattt tgaagagctg ctggcgcaac
    26041 ttcttctttt tcttcagcca cttcagcttg ttttgcagct gcagttgcgt ctacttcatc
    26101 ttcgtaacca aacatgtaag taagagcaaa accaagggca aatgatacag ctaccataag
    26161 aaggtattgt ggaagttgtc cgttaccaac ataaagcatt gtaccaggga tgatggtgat
    26221 accattacca gtaccagcaa gtccaaggat agaagccaat ccaccaccga ttgcaccagc
    26281 aatcaatgaa aggaagaatg gtttacggaa gcgcaagttc accccgaaga tagcaggctc
    26341 tgtaatacct aggaaggcag aaagagcagc cgggaaagca agtgttttca gttttggatt
    26401 ttttgtttta acaccaaccg caacagtagc agcaccttga gctgtcatag cagctgtgat
    26461 gatagcgttg aatgggttag catggtcagc agcaagtaat tgcacttcaa gcaagttgaa
    26521 gatgtggtgc acacctgaca cgacgatcaa ttggtgaacc ccaccaatca agaaaccacc
    26581 aagaccaaat ggcatgctaa gaatcgcttt tgtagcaata aggatgtagt tttcaacaac
    26641 gtggaaaact ggtccaatga caaagagtcc aaggatagac atgaccaaaa gtgtcacgaa
    26701 tggtgttacc aagaggtcaa tgacatctgg aacaaccttg cggacagctt tttcaaattt
    26761 agctccgaca accccgatga tgaaggctgg aagaacggaa ccttgcaaac caacaacagg
    26821 gatgaaacca aagaagttca tcgctgttac ttcaccacct tgagcaactg cccaagcgtt
    26881 tggaagtgag ccagagacaa gcatcatacc aagaacgata ccaacggcag gatttccacc
    26941 aaatacacgg aaggttgacc acacaaccaa acctggcaag atgatgaagg ctgtatctgt
    27001 caagatttgt gtgtaagttg caaagtcacc tggaagtggc atttcaagag cgttgaaaag
    27061 accacgcaca cccatgaaga gacctgtcgc tacgataact gggatgattg gaacgaaaac
    27121 atcaccgaaa gtacggatag cacgttggaa ccagttccct tgtttagcaa cttctgcttt
    27181 catgtcatcc ttagatgatg ttggtaagcc aagtacaaca acttcatcgt acattttgtt
    27241 aactgtacct gtaccaaaga taatttggta ttgccctgag ttaaagaaag caccttgaac
    27301 tttttccaag ttctcaatca cttctttatt gattttctct tcatctttga ccatgacacg
    27361 tagacgagtc gcacagtggg caacactatt gacattttca cgtccgccca aggcatcgat
    27421 gacttttttt gcaatttcct gattgttcat ttgcaaaaat ctccttatat aacattttgt
    27481 tcttgtttga aagcgatttt attcgccctt acgactatta ttttatcatg ttttaaaaat
    27541 atgtcaagcg ttttgcagaa aaaatattct atccacttag tgggcaagtt ttagattgat
    27601 tgtttttgac tgtttcatca ggaattcctt gaaaaataag tttgaaacct tggttaaaaa
    27661 gtcttttatt tcattatctt tcacattttc gtaaaaattt gattgatttc ctgtatttat
    27721 tttatgataa tcgtttgaca gtttttcctc ttttttaaca taaaaggctt gcatttttta
    27781 tcgaaaacag ttactattaa aagtgataag atttttggag gaatgaatgg aatggacaac
    27841 tgagcgtcgt tacagacttt atcaagattg gacgcaagaa gaaattcaac atataaagga
    27901 aaatatggca caatctccat ggcatactca ttaccatgtt gagccaaaaa caggacttct
    27961 caacgaccca aatggctttt cttactttga tggcaagtgg atcctctttt accagaattt
    28021 tccttttggt gcagcccacg gtttgaaatc ttgggcacag ctagaaagtg atgatttggt
    28081 tcactttaaa gaaactggaa tcaaagtttt accagatact ccattagata gccacggtgc
    28141 ctactctggt tctgccatgc aatttggcga taacttattc ctattttata caggaaatgt
    28201 tcgcgatgaa aactggatcc gtcacccata ccagatcggt gctttgatgg acaaggaggg
    28261 taagattaca aagattgaca agatcttgat tgaccagcca gcagactcta ctgaccactt
    28321 ccgcgatcca caaattttta actttaaagg tcaatactat gctgtcgtcg gtggacagga
    28381 cttggagaaa aaaggcttcg tccgtctcta caaggctgtc aataacgact acacaaactg
    28441 gcaagcagtt ggcgaccttg actttgctaa cgaccgtact gcctacatga tggaatgtcc
    28501 taatctggtc tttgtagagg aacatcctgt ccttctctac tgtccacaag gattggataa
    28561 gaaagttcta gactacgata atatctttcc aaatatgtat aagattgggg cttcctttga
    28621 ccctaaaaat gccaaaatgg tagatgtgtc tcaacttcaa aacatggatt acggtttcga
    28681 agcctatgca actcaagcct tcaacgctcc tgatgggcgt gctctagcag ttagctggct
    28741 tggtttgcca gatgtttctt acccatctga ccgttttgac caccaaggaa ccttctcttt
    28801 ggtcaaggaa ctcactatca aagacgacaa gctctaccag tatccagtcg ctgctattaa
    28861 ggaccttcgt gcttctgaag aagccttctc aaaccgttcc caaaccaaga acacttacga
    28921 acttgaactc aacttggaag ctaatagcca gagcgagatt gtcttacttg ctgataaaga
    28981 aggtaaggga ctttcaatca actttgacct tgtaaacggt caagtgacag tggatcgtag
    29041 ccaggctgga gaacagtatg cccaagaatt tgggacaact cgttcttgcc ctatcgagaa
    29101 tcaggctact actgctacaa tcttcatcga taactctgtc tttgaaattt tcatcaataa
    29161 aggagaaaaa gtattttctg gtcgtgtctt cccacatgcg gaccaaaatg gtatcctgat
    29221 caaatctgga aacccaactg gaacttacta tgaattagat tatggtcgca aaactaactg
    29281 atgtcgccaa acttgcaggc gtcagtccta ctaccgtttc tcgggttatc aataaaaaag
    29341 gatatctatc tgagaaaacc atccaaaaag tcaatgaagc catgagagaa ttaggctata
    29401 aacccaacaa cctggctcgt agtctgcaag gaaaatcagc taagttaatc ggcttgattt
    29461 tccccaatat ttccaatgtt ttctatgcag aattgattga taaattggaa caccaactct
    29521 tcaaaaatgg ttacaagacc atcatctgca acagtgaaca tgattctgag aaggaacgcg
    29581 aatacatcga aatgttggaa gccaatcagg tagacggcat catttctggt agtcacaacc
    29641 taggaatcga agactacaat cgtgtgacag cgccgattat ttcctttgac cgaaacctat
    29701 cgccagacat ccctgttgtc tcctctgaca actatgctgg tggggttctt gctgcccaaa
    29761 ccttggtcaa gacaggtgcc cagtctatca tcatgattac agggaatgac aattccaatt
    29821 cgccaaccgg actgcgccac gctggttttg catccgtact cccaaaagct cctattatca
    29881 atgtttccag tgacttttct cccgtcagaa aagaaatgga aatcaagaat atcttgaccc
    29941 gggaaaaacc agatgccatt tttgcttcgg atgatttgac agctattctg gtcattaaaa
    30001 tcgctcaaga attgggcatt tctgtcccaa aagagctcaa ggtcatcggc tatgatggga
    30061 cctactttat cgaaaattac taccctcaat tggctactat caagcaacct ttggaagaga
    30121 ttgcttgtct cactattgat cttctcttgc aaaagattga aggcaaggaa gtcgccacaa
    30181 ctggttactt cttaccagtt acgctattac caggaaaaag tatttaaaca caagaaaact
    30241 cagaccgatt cgtctgagtt tttatgatct taaattttcg agatagcgct gggctgtctc
    30301 taggttaaag gttttatctg agatgaggcg ctctactagg ggagcaactt cagattcact
    30361 agccccagct aggagagcta gggatttggc ctgtagtttc atgtggcctt gctggatgcc
    30421 cgtacttacc aaggctttga gggctgcaaa attttgagca agaccgatgg acacgataat
    30481 ctgggctaat tctctggcag aaggatttcc tagtagatca tgactgagag ctacacgtgg
    30541 gttgaggccg atagagccac ccttagtcgc tacaggcatg ggcagggtca tctcaccgac
    30601 caattcttct ctttcaaggt ccagcgtcca gcagctaaga ccttgatagc gtccatctcg
    30661 actggcaaag gcatgggccc cagcttcgat ggcacgccag tcattaccag tggcaatcaa
    30721 aatcgcatca ataccattaa aaattccttt attatgagta gcagctcggt aaggatcagc
    30781 ctgcgcaaac tgactagcca acgcaatttt ctccgcaatc tctcgtcctt gatccttttg
    30841 gcggctcaag tagcgaaagg cgatgcgaca gcttgcagtc accagagaat cagtcgcgta
    30901 gttggacagg attcccatga gactctgtcc ctgactgagt tcttctaaga ctggtttcaa
    30961 ggcttccagc atggtgttga gcatattggc acccatggct tcctgggtat cgacatgaat
    31021 ataaacaacg agaaagtctg gttcgccttt tatctgctcg acatgcagat cacgcgcccc
    31081 acccccacgt ttaacgatag aaggataggc ttgattggca agctccaaga gctccgcttt
    31141 cttgctggca atcttctctt gcgctagttt aggattagca acttgataaa gggctacctg
    31201 cccaatcatc tggcgctgat ggacttgtgc agtaaaacca cctgcacgct tgatgatttt
    31261 gctggcatag ctggccgccg caaccacaga gggttcttct gtcacatagg gaacggtgta
    31321 ttcctgaccg ttgacaagta cctccggaac cagtgaataa ggcagagaaa aagttcccac
    31381 tacattctca ctcagctggt ctgccacagt cacgctcatc tgttcatcct tctccagact
    31441 agcttgtctc tcaggactaa ggagcgcctg agcttttaac agctcgaggc gctcttggta
    31501 tgatttttta gaaaatccat tccaacttat cttcattatt tttcaacctt gctataacgg
    31561 cgttggtggt cgagaatttc aaccaaggca aaatcttgat tttcatagcc agcaaactgg
    31621 gcagagtttg tttcatccaa gtttacttcc tcaaaaaaga ccttttcata gtctgcaacg
    31681 gatagggcag ttcgttggtt gagcttgttc aaacggtctt tatccaaata agcttcatat
    31741 ccttcaacca attcaccact gaagaactca gccacagctc cacttccgta actataaagg
    31801 gcgattttat ccccagcttt caagctatct gtattttcca agagagacaa aagtccaagg
    31861 aaaagtgaac ctgtgtagat attccccacc ttttgactgt agagaataga ctggtcaaaa
    31921 tgcttttgta agaggtcttt tttctcttga ggcaggctct tatccatgat ttttttcaag
    31981 ccttttagcg ctaatttagg ataaggcaag tggaaacaaa cagccgcaaa atcatccaaa
    32041 gtaagctggt agcgtttttg atattcaagc caagtcgttt tcaaactatc caagtattgt
    32101 tgggtagaat agacaccatt tacataagga gttgtcgagt aatttggtcg ccagaaatcc
    32161 atgatgtcac gggtctgagc tacattgtca ttattaaagg ccatcatgcg tggattttgt
    32221 gtaatcaaca tagccacact tccagcacct tgagttggtt ctcccggagt ttcaatacca
    32281 tatttggcaa tatcactggc aatgaccaag accttggact ccggagaatt ttccacatgc
    32341 aatttggcat aatggagggc agctgtcgcc ccatagcagg cttctttaat ctcgaaacta
    32401 cgagcaaagg gctggatgcc cagcaagcca tgcacaaaga cggccgcagc cttactctgg
    32461 tcaattcctg actcggtagc cacaatgacc atgtcaactt cttgtctttc ttgctcagtt
    32521 aaaatagagt cactagcact ggccgccaag gtcacgatgt cctcagttag gggcgcaata
    32581 ctcaattcct tgagtaagag tcctttactt aatttttcag ggtcaattcc cctcgcttct
    32641 gctaagtctt gtaatttcaa gacatattga ctggtcgcaa aaccaatctt atcaataccg
    32701 attgtcatat ttacctctgt tttatcattc atgtaaaaaa tcgttctata ctattttatc
    32761 acaaatggca gtaaaagaga gaaaaaagac ttgattcacc aaatcaagcc tcttattggt
    32821 catcatttta aagaatgatt agttgctaga gagttcaccg atataagtag ctttataagc
    32881 tccattcaca gttatcagct cctggaggat caaatttcct gagtaagtcc ttcccatctc
    32941 atctacaaat ttttgataaa actgactggt cggaatttct ctgacatcct tatcaaatgt
    33001 cttgtcaagt gttttactaa ccttctcagc aatcaattga tgctcttgcc atccactttg
    33061 aaactctgag ccccaactag aaaccatgac tgggataaac aacaaggtca gtagacttac
    33121 agacaataag gaaaatagta gacttcctgc aaaactagaa tcctagttca tgattgataa
    33181 tgccagcaat caaattcatt cgtaatccga agcgtttacg atgatttcga tagattgttg
    33241 aaaacatttt aaacgttttt actttggcaa agatgttctc aaccttgctt ctctccttgg
    33301 atagcgcatg gttacaagct ttatcttcag ctgttagcgg cttgagtttg ctggatttac
    33361 gtggagtttg tacttgagga tatatcttca tgagcccttg ataaccactg tcagacaaga
    33421 ttttaccagc ttgtccgata tttctgcgac tcattttgaa caacttcata tcatgacaat
    33481 agttcacagc gatatccaaa gaaacaattc tcccttgact tgtgacaatc gcttgagcct
    33541 tcatagcgtg gcatttcttt ttaccagaat cattcgctaa ttctttttta gggcgattga
    33601 tttgtacttc cgtcgcatca atcattaccg tgtcctcaga actgagagga gttcttgaaa
    33661 tcgtaaaacc actttgaaca agagttactt caacccattg gctccgacgg attaagttgc
    33721 tttcgtggat accaaaatca gccgcaattt cttcataagt gcggtattct cgcacatata
    33781 gaaagcgttg ttaatttatt tatctcattt ttcagaaaat tctttcattt gtgtaaagtc
    33841 tacgatactc gatgtgtttt tatataatga tacagtctga gaatcactgt tccgctagcc
    33901 attccaatag agattaccaa agccaacatg acaaccaagg tcgcacttgc cagtgcttta
    33961 ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg agagataacc tggaaccagc
    34021 ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct tataaagaat acttaaaatc
    34081 tggctgacac aagaaccaat aatggctgca atgaaggtag ctacaatgac attggtcggc
    34141 tccttgagca agagatagat tagccagaca gtcatgccca aaatccctcc aggtaagagc
    34201 atagaccgtt gcacattgag tacgattaaa aaagtgataa tggcaagaaa acttgctact
    34261 gcttgtaata aaaaggttgt tagtgtcata ttagttcatc aataccaagg cgacagaagt
    34321 tcctgcccct aaagcgaggg taatgagcag ggattcaaac atcttactca taccagagtt
    34381 tatgtggttg gtcataatat cacggaccgc attggtcaag gcaatacctg gtacaaacgg
    34441 catgaccgca ccagctataa tcaaatctgc cgttgaagga aaacctgtgt agcgagccca
    34501 aaactgggca attatcccaa agacaaaggc tccagcaaag gctgtcacaa agggaattcg
    34561 gataaatttt tccacataga gggaaaaggc aaaaccaaat aaggtcgcca ctcctgcccc
    34621 aagtgcgtcg tagatatttc cgctaaacat aactgaaaag aaaggagcgc taaaggtcgc
    34681 agccagagtt acctgcaact tagtataggg aaggggttga gcttgcaagg ccgtcaattg
    34741 cttaaaggct gtttctaaat caatctgccc cccaaccagc tgacgagaaa tctggttcac
    34801 atcgcagact ttttcgatgt tataagaaga ggaggtcacg cgcttcatgc gcgaaatatt
    34861 ggtattttca atagagaaaa agatagcggc aggcatggca aggacattgc aatccacaat
    34921 cccctgcgaa tgcgcgattc gaatcatggt atcttctaca cgatggattt ctgagccact
    34981 tttaaggaga atagtccccg ctagcataat cacatcaatg acggtattta attcttttga
    35041 ttcttccatg ctttcctcct tttatcaact ccctctattc tatcacaaat ccggactcaa
    35101 aaaaaatctt tgccatgaaa tcatgacaaa gattgattac tcattttgat tatccatctg
    35161 cttgattatc catctgcttt taaggagtag ctgaagttgt tttaggtttg tagattgaaa
    35221 tcttgactct agtcttattg aggtctacct tttcacctgc tctaggactt tgttcaacaa
    35281 ccatgccttc tgcactacct gcaggcgctg tcgtcacttc tacaacttct atattagctt
    35341 ccttaatccc aacaatttga atcaaattgt tcttagtaaa ctccaagctg gaaccaatgt
    35401 aactcggcat ggcaacactt gtaacttttt tagctactgt caagacaatt tgaataggtt
    35461 tactcacatc ataggtcgtt ccggcacctg gactttgttt cataatcgtt cctggttcgc
    35521 tttcgctgga ctcttcttcc tctatcttaa tcaaattctc aggaaccttc ttctgcttga
    35581 gttctgagat tacttctgta gagttccgtc caatatagtt ccctaattga atcgtcgtag
    35641 cttttttagc tactgtcaaa acaatttgag ttgccttgct caagtcatag gtcgtacctt
    35701 ctggtagact ttgcttcagg accgttccag cctcactctc attggactct tcttcctcaa
    35761 ttttaatcaa attatctgga acttttttct cttttaattc cgcaatgaca tcagaggatt
    35821 tccgaccgac ataattacta atttggaagg attgcttgcc tgatgagaca accaaattga
    35881 ttttcgttcc ttcttttcga ccagttccag cgccaggatc tgtacggata atccgccctt
    35941 cttccacctt ttcactagcc tctgtcttct cctcaccaat ctcaaaattg gcttttttga
    36001 gcgttgcctt ggcctctgca actgtctgac ctgccacatc tggaatggca atggttgcag
    36061 gagttctgga tagtatccaa ataagagaag ctgccaccaa tacaaggctg gccaacaaaa
    36121 tcaggtaacg catcttaaat ctatgttttt tcggtgcttg tggttggtaa gtttcctctg
    36181 tcacagcctg gcttggggtt ttgattgatt tgtgttctgt ctgcgcttga accttaggaa
    36241 tagatgtcaa ggtactctga gaaaccttcg gcaaggtctt ggtatctgcc ttgctcgttt
    36301 catcaaagat taacttactt tcatttctac gattgtagga caagctacta gacaagtcca
    36361 catacatctc tgaaaccgag cggtagcgat tggtcaactt tttagcagtt gccttgataa
    36421 taacattttc taaagcctga ggtacagatg gattttctgc aataacggac ggcaggggtt
    36481 tctggaaatg ctggagggca atggtcaccg cgctatcccc gtcataaggg atatggcctg
    36541 tcaacatctc atagaaaata atccccatag catagatatc actctgcaca gtcgccttcg
    36601 aaccacgcgc ctgctctggg gacaagtaat gaactgagcc caacatcgag ttagtctggg
    36661 tcagacttgt ctctgcaaag gctacagcaa tcccaaagtc tgtgaccttt gcagtcccat
    36721 ctggtgtcaa aaggatattt tgaggtttca agtccctgtg aacaattcct ctggtatggg
    36781 ccaaacgcat ggctaggagg atttgtccca tgatacggac tgcttcttca ttagaaagag
    36841 gataatgttc cttgatatag cgtttgaggt ctagtccagc aacatactcc attgcaagat
    36901 actgttgacc gtcttcctca ccaatatctg ttatccgaac gatatgagga tggtctagat
    36961 ctgccatagc tctcgcttca cgctgaaaac gagctacagc tatcgggtcc gtctggtagt
    37021 tggtcctcag aaccttcact gccacttctt ccccatctaa gattaagtct ttggctaggt
    37081 agacatccgc catacctcct cgaccaatct gtttgacaat ccgatagcgt ccggcaaaaa
    37141 tcttgccgat ttggatcatt ctgcatcctc ctcgttcata gaaacaaggg caaccgtaat
    37201 gttgtctaaa cctcctgcat tgttagcaaa acgaacaagt gtctccgttt tatctgctaa
    37261 aggaatatca ctggttacaa tatcacgaat ctcactgcct gaaatcatgt tggtcaagcc
    37321 gtcactattg agcaagagat agtcacctga ctcaaggata actgtcccaa aatcaggctg
    37381 aatttcatct ttttgcccaa tagactgggt gataatattt ttttgcggat gagcttctgc
    37441 ctcttctggt gtcaattgac cagccttgag caattcatta accaaggaat gatcgctcgt
    37501 caactgatgg tattcttctc cacgaatcaa gccgatacgc gaatcaccaa tatgagcata
    37561 gatagcctga ttatcaataa tagcaaggac ttccaaagta gttcccatgc ctctgtaagc
    37621 ttcatcctga ccaagctggt gaatcttttg attttcaatt tctaggtaat gggcgaacca
    37681 ttcacgcact tcattgactg tatcgatctg ggtatcaacc caagctacac ccaggtctgt
    37741 gaccgccatt tcactagcga tattccctgc acgatgacct cccatcccat cagctaaaat
    37801 aatcatggta cgtccagctc tattgacata gtggttgaca tagtcttggt tatttgttcg
    37861 tttctgacca acatctgtta ataatgaaat ttccatgtgt cagttccttc ctaatccgat
    37921 atcttgcgaa attgactgat gaagaatcca tcacttccat acaattcagg tgtaatgagg
    37981 atacagccgt ctttcatgat atccttacat tcatgttcta gttttacctg ctcgaactcg
    38041 ggatgactct ctaaaaaggc cttaacgact tgaaaattct cctctgagac gatagtgcag
    38101 gtgctataag ttattatacc acctttgcct agtatttgac aaacactacc taatatttct
    38161 aactgaattt cctgcaagga cgcgaaatct gccgtttctt tattgtattt gatatctggt
    38221 tttcggcgca aaagaccgat tcctgaacaa ggagcatcca ccaaaatctt atcaaaggaa
    38281 tcctggtcaa aaaactcatg cacctttctg gcatccaatt tttgagtttg aacccgatct
    38341 tcaactccca gacgttgggc attttcttga attaaatcca acttgtggtc gtacaagtcc
    38401 agagcagtaa cctgacctgt cgtaagataa gaggctatat gggctgtttt cccacctggc
    38461 gccgcacagg catcaagcac tcgctcatca ccttgtaaat caagcgtcgg agcaaccagc
    38521 tgactggact cgtcttggat ggtaatggct ccatccgcaa acaaattatg ccctgcaaaa
    38581 tgcccctgct ccttaaccag accagtggtt gctaaaaggg aatcattcgc ctccaacaag
    38641 gcttggattt cctcttttcg acttaggtct gttacacgaa tactggcttt gtttcgcact
    38701 aacaggcttt caaagatggc ttttgctctc tcctctccgt attcttcctt gagtttggca
    38761 actagccaaa ctgggagaga ataggcaatg gagtcacgct tgttttttcg cttgatgcta
    38821 gcaatatctg gccagccttc acgcaagata cggcgaagga cagcgttgac caatttttca
    38881 ctgccttttt tacggagttt ggctaattcc actgcttcat tgaccacagc atgatctgga
    38941 attttgtcca aatagcggag ttggtaggca ctcatgagaa gaaggacata gagccagctg
    39001 tctaactggt ctctgtcttc gataaagtgg gataggtacc attccagagt cagtttacgg
    39061 gctaccgttc catagactag ctcggtcact aagcccttgt ctgctgcaga gagctgactc
    39121 cccttgaggt gtttatttaa ggcaatattt gaatatgctt gattcacaaa aacatcctct
    39181 agcactgcta gagctaaact tctagccgtt tctactttag tcaccaaatc gttctcctac
    39241 agtcaatgta cgtccaactc cattgaggaa ggaagcaatg tccatcttag gcttaccagc
    39301 tggctgcact tgtttgaggg atagagcccc ttcagccgtt gcgacaatca attctttctt
    39361 gccgatagag agaatctcac ctggatttcc ctgaccttct actggtaggg cttcataaat
    39421 cttaaagcgg tcgcccttaa ggaaagtatg ggcaacaggc caggggttca ttccacgaat
    39481 ttggttaaag agttgacgat tggttttgtt ccagtccagt ttttcttcct ttggctttat
    39541 atttggagag aaggtaacct gactcgtatc ctgcggttca ggtttgatat caccagcaat
    39601 ataggcaggc agagtgtcca aaagcaaatc acaaccaact agcgccaatt tttcaaacaa
    39661 ggtgccaaca ttgtcctcat ctgtgatcgg aatgctgcga cgagaaatca tatctcctgc
    39721 atccatttcc ttaaccattt ccatgatggt cacaccagct tcctcatccc cttgaatcaa
    39781 ggcataatgg ataggcgcac caccacggtg tctaggaagg agggaggcat gaacgttgac
    39841 agcaaagtcc atgctatcaa ggagtttgct tgggagaaac tgcccaaaag cagcagtcac
    39901 aattccatct gctcctagct tcataagatc ttccatctct ggacttccag ataatttttc
    39961 aggttggtag atagatagtc ctgcttcctt ggcagcctgc ttgactgggg tttcttggat
    40021 aactttttta cgaccaacag cacggtctgg ctgggtcaca acggctagaa tttcgtaacg
    40081 gtcatctgtc aaaagtcctt ttaagactgt tgctgaaaag tcgggggtcc ccataaagat
    40141 tagttttgtc atatcttctc cttcttataa aaattgctgc ggctcatggt caatgctgag
    40201 acggagctca ctattttccc gttcttgagt caaggccaag acctggttga gggtcggccc
    40261 cagctcatct tctaaacggt atttaattaa aatctggtaa tgatagaggt tgtgggtacg
    40321 ggcaatcggt tttggcgttg gccccagaat gggactggtc tctgacaagc ctgaccgcaa
    40381 aatgttcatg acttcatagg cacgtttgaa aacctcttct tctttcttgt gagaaagggt
    40441 aataccaatc gtgaaatagt aaggtggata gccgagttgt cgtctgattc ccatttcata
    40501 ggcataaaag ccttcgtaat cttgatcctt ggcaaatcga atagcatagt gctgcgaatt
    40561 gtaggactgt atcaagactt gacctgcttt ttcagcccga cctgctcgac ctgccacctg
    40621 agtcaagagc tggaaggttc tctcagaaga acggaaatca ggcagattca aggccgtatc
    40681 cgcatttaga actccgacta gggtaacatt gggaaaatcc aaaccctttg caatcatctg
    40741 agtaccaagt aaaatatccg cttcccctcg cccaaactgg tcaagcaagg cttggtgact
    40801 gcctttcttt cgagtcgtat ccacatccat cctcaaaatg cgagcttggg gaaagagttc
    40861 tgctagctca tcataagcct tctgagttcc cgtcccatag taacgaatac tgcggctctt
    40921 acagttagga cagacctgag gaatatcctt cgagaaacca caataatggc agttcatagt
    40981 cttggtatcc atatgcaagg tcagagaaat atcgcagttg ggacaagtat ccaccgtccc
    41041 acactcccga cacatgacaa agctagaata accacggcga ttgagcatga gaaccacctg
    41101 ctctttttta accagacggt cttggatagc ctctagcaaa ggaggcgtaa agtttgacgt
    41161 ctcattttgt ccgatatagt ctcgaaagtc aatcacttga acctcaggga ttgtagccaa
    41221 aggattggca cgttgggtta gacgtaagtg tcgatagacg cctttgccag cacgtgcccg
    41281 gctctctaag ctcggcgttg cagatccaag taccagagtt gcttgattat actgagcccg
    41341 taaaatagct acctctctgg catggtaacg gggattgctg tcctgcttat aagccgcttc
    41401 atgctcttca tcaataatca tgacacccag atttttcaga ggagcaaaga tagcagatct
    41461 ggcaccaaca acaacttggg catcgccacg ctccacctta cgccattcat catacttttc
    41521 gccattggat aatcctgagt gaagaatggc taccttgtcc ccaaaacgtg ctataaaacg
    41581 ctcggtcatc tgaggagtca aggaaatctc aggtaccagc aaaatagctg tcttaccctt
    41641 atccagggca ccttggataa tctgcaagta aacctcggtc ttcccacttc ctgtaatccc
    41701 ttgaagtaga aagggaggtt gagaactgcc aatagaactc acaaccgcat cacgcgcctg
    41761 tctttgttct ggatttaact ccaaaggtct acttgcttca attccttcaa aataagcagc
    41821 cgagcgttga acttcctttt ggactatggt aacagcacct tgatccacaa agaagttgac
    41881 ttgctctcgc gagtaggact ctaacaagct agccaaggaa gcgctctctg gatgagacag
    41941 cagataatct ctcagttcca actttttctt ggcacgtgta gaaatctcaa caccttctaa
    42001 ttgggcatgg tcaacctcat accaagactg ggtcttgacc ttcttttgat cgactgcctg
    42061 atattccaga ccaagcaggc cttttctagt caaacgcatc atttcagctt gcttggcaag
    42121 gtctagtgaa gaaaaggcta gcgaatcttc tgaaccaaac aggcgtactc gttcttcctg
    42181 actcaagcct tccagaggat agagaatctt gtcatagcta gaattcagaa accctggaag
    42241 catggccttg aggatagaga ttttgtagga gaagacagat ttgcgtaact cctcagccag
    42301 ccagagttgt tctggcgtga gaacaggaga aaaatccagc acctctgcaa tatcttttaa
    42361 atcttgctcc atctcttctc catctgattg ggacttcaaa ccaagaacaa tcccttgaat
    42421 caggcgattc cccttaccaa aaggcacatg aacccgcatc ccaacttcca gcattccctc
    42481 aaattcctcc ggaatcctgt aactataggg ctggtccgtc tgcatcaagg gcacatctac
    42541 gataatctta gctagggcca tcttctcacc tcctccttgt cagtacattc ttgcaataga
    42601 aaaaataaga ttgagtcccc ccaaccttaa attttttcac catcttcttt ttctttagca
    42661 atttgctctt tgattttctt ttcttcttct tctttgcggc gtttttcttc ttcgatacgg
    42721 cgacgcactg cttcacgttt tccttctgga tctgggtgaa ttgtaacgtt tcctgattcg
    42781 atttcttcta aagcgcgaag agttgatttt tcagacttga aaccttgagt tgctggggca
    42841 cctgcttcca attcgtgggc acgttttgct tccaagatta cgagtgaata ttttgaagga
    42901 accttgtcga gcaaggtatc aatagagggt tttaacatca tttgcttgta cctattttct
    42961 aaattttatc gggtagttgg agattttggt aacatctcct gatagtgacc aatgacacga
    43021 tccacacaga agtgttctgc ttcaatcaca catttgacac gttcagcagc taggggcacc
    43081 tgatcgttga caatcgcata atcatactca cgcatgaggg caatttcttc cttggccttt
    43141 tcgattcgtt gggcaatcac ttctgcacta tctgttccac gacctaccaa gcgatcttgc
    43201 aattcatcca aatctggtgg tgtcaggaag ataaagacag catctggaac ctttttcttg
    43261 acctgaagag caccctgaac ttcaatttca aggaaaacat cgattccctt gtccaaggtt
    43321 tcattgacat aggtcagagg agttccatag tagttgccga catattctgc gtattccaac
    43381 atctgtcctt gacgaatcag ctcttcaaat tcttcacgag tacggaagaa atagtcaaca
    43441 ccgtccactt ctccaggacg ttgtgcgcgt gtcgtcatcg atacagaata ttgaaattgg
    43501 ttttcagaac tctcaaaaat ctctcttcta accgttcctt ttccaacccc tgaaggacca
    43561 gaaaaaacga ttagtaagcc tcggtctgcc attgtgtctc cttttagtca atctgtgaaa
    43621 taacatttct ctagaataat ggcaaaaagc cagattatcc tttacagtct ttctatctag
    43681 tgtaacaaaa aagcagtaat ttttcaactg ctctttctta tttatttagc ataatctact
    43741 gcacgaagct cgcgaatcac ggttaccttg atatttcctg gataatcgag attgttttca
    43801 attttcttac gaactttgtg agccaagatt gtgactttgt cgtccttgat ttttcctgga
    43861 ttgaccatga tacgaatttc acgtcctgct tgaagggcaa agctagtttg cactccttca
    43921 aagccgttag caatttcttc caaatcatgg agacgcttga tgtagctttc aagagactca
    43981 ctacgagcac ctggacgggc tgcgctcaag gcatctgctg cagcgacgat aactgctatc
    44041 acgctctcag cttcaacatc tccgtggtga ctagcaatcg tattcaccac aactgggggt
    44101 tccttgtact tacgggccaa ttccataccg atttcaacgt ggctaccttc aacctcatgg
    44161 tcaatggctt tcccgatatc gtgaaggaat ccagcacgac gggcaagagc cgcattttca
    44221 ccaagttcgc tcgccatgat accagccaac ttagcaacct caatcgaatg gcgcaaaaca
    44281 ttttgtccat atgaagtacg gaactgcaaa cgtcccataa tcttcatcaa gtctggatga
    44341 aggtttggcg caccaatttc ataggcagca gcctcaccgt attcacgaat cttattgtca
    44401 atctcttgac ggtttttctc aaccaactct tcgatacgag ctggatgtat acgaccatct
    44461 ttgagcaaca tttccatagt catacgggca atctcacgac gaatcggatc aaatcctgac
    44521 aaggtcacca cttctggtgt atcgtcgata atcacatcga cccctgtcaa actttcaaag
    44581 gtacgaatgt tacgaccttc acgaccaata atgcgtccct tcatagtatc gtctggcaga
    44641 tgaactgttg agtttgttga ctccgctaca tattcaccag cgatacgttg catagcttga
    44701 accaagatgt ccttggccat tttgtcagaa cgttccttga cctcttgctc agcttcgcga
    44761 atgcgactgg caatctccct ggtcaagttt tcctctgtct gagccaagat aatatctcgt
    44821 gcttctgcct gagacagagc accaatacgc tctagttctg cttctttttg tctttcgact
    44881 tcctctaatt gctcttcacg cgcatcaagg tttttcgctc tatcagaaat actttgttct
    44941 ttttgttcaa gtgtttgttc tttactcgtc aaattgtcgt ccttacggtc aaggctagta
    45001 gctctctctg tcaaacgact ttcgatttgt ttgagttctt gacgttctga tttgaattca
    45061 gcgtccactt cttcacggta ttttctggct tcttctttgg cctccaatag tgcttctttt
    45121 ttaagagact tgctttcacg tttggcttca ttaacaagta aatccgcttc acgctcagct
    45181 tgtccacgta aattagttgc ttcttgttca gcatttaaaa gcatcaactc tgcagcttcc
    45241 tgagatgatt tcatcttagc tgagatgctg acatatccaa tgactaaacc aatgatgacg
    45301 gcaaaaacag caatcgcaag cgacatgatt tccatgtttt tacctcattt tattgttatt
    45361 ccgaatgaca tacattcttt tacattctac cataaaaaag tgattttcac aaacctaaaa
    45421 tagaatatgt tttggggaat ttggaacaca tttaccaaaa taaacttgtt gtttagaaat
    45481 agtagtttag tagagacttg agaaaaagcc tactttgcag tagacttcct aatgattatg
    45541 caatacaaga agtttgtttc taacaacagc aagatttcac tcaggtattc tctgtaaatt
    45601 agactagtgt cctgcgcgaa ttttatccat tcctcgcaaa accttgtctg ataactcctt
    45661 attttgggcg attctcaggg tttcctgaat actgtcccat tcgctctttg aaagaactac
    45721 aatgtcctca tctggatttt tattgaccac cgtcaaaggc tcaaattcat catttacctt
    45781 cttcatgtaa tcctttaaat gatttcggaa tgttgagtaa agaactcctt ccataaccat
    45841 acctcgtttt agctcttttc cactattata cacgaaaaga aagaaattgt caggaacctg
    45901 tacaagattt tcttttctat ctatttatac tcaatgaaaa tcaaagagca aactaggaaa
    45961 ctagcctcag gctgtacttg agtacggcaa agcgacgttg acgcgatttg aatttgattt
    46021 tcgaagagta ttattcgtaa aaaatctcaa aaagcctacc ttttagtaga cttagtttgt
    46081 ttctattcta atcggcactc ttccaaaatt ttgctctgct atacttggct ttcctagttg
    46141 gtaaatctgg tctgcttttt gggtcatagc atcccaaggt tctttgccaa ttcggctgac
    46201 tagattgacc tgtcctttca gagacttgag atgttgcctg cctttttcag taaatccaag
    46261 gacatgaatg gcttctggca agacattttc cctagcctgc accaaaatat aggtcaatag
    46321 gcgtctgaca cgcgccttgg tgtaacgttt ggtagtcact aactcgacca attcttccac
    46381 agactgggct gttttaatag cttctttaat gcgcacagcc atttcttgat tgacctgata
    46441 gatggtggtt aggtcgggat ttgacaagat ttgatagcgg agcaagggaa aatagtcttc
    46501 ccagatcacc ttactggcct gctcaaatag ggcaacagaa ggcataaaac gttctaagaa
    46561 atcttggtcc ttctgatgtt gacggagggc tgtcgccgag gcaaagtcca catctttatt
    46621 cacagaatgg taacctgccc cctgacgctg aatcggatgc agcttgatat ttcgtcctgc
    46681 aaccgccttt gcataggcca gagcaagaac atgattgggg gtattacctg aaaaatcaag
    46741 accagcaaat tccttccaca tagattgggt tttctgggga taggaaaggg aatcaggcag
    46801 attttccaca aatttctcca tctcagcacc tttctctgtg tataagtcag cgattttctg
    46861 ataatctcga acttcctctg tcccaaaaac tagactatca atgcccaagc gatccaagat
    46921 atccacagct ccttgaccaa agaaatcagc tgcctgaaca ctgactaaaa agggcaattc
    46981 cactaccaag tctgcgccat tttccagcgc catctgggcc cttgtccact tatccacaat
    47041 agcaggctct ccacgctgca tgaaattccc tgacatggca acaattttca gtccctctgc
    47101 ctgatccagc aggtatttat ggccattatg aaaaggattg aactccgcga taatacctgt
    47161 gatggtcatg ctaatctcct acttctgcgc cacaaaaaac caacgggtgc tagtttctgt
    47221 tggttcctta tcctcaaagt ctgcatagag tttgaaggac ttgaatccag cctgttccag
    47281 taagatatca taggtcaaga tctcataggt cctctcctca tgtacttcat cgtggcgact
    47341 aaaggaaccg tcagcctcct tgataaagaa agtcagctca tgcacgatgg agtgaggagc
    47401 ttcgtcctca tatgtatccc aaagcatggc aaaatcttcc gcattttcat ggtaggaata
    47461 gccagggaat acttcatctg tctggtaggt cgagtgcacg tcaaagatga aaactccctc
    47521 ttcattcagc gcattgtaca cgtccttaaa gacgtcccct acttccacct catcctgcat
    47581 atagcagata gagtccgaat aacacgtgac aaaatcgtat ttccctgcct tggacaaatt
    47641 cagcatatta ccttctataa aagcaatctt ttgcttggct gaagttgctc tcttctccgc
    47701 aatcttcaac atatccgcac tcaagtcaag tccagtcaca tcaaaaccag cctgagagaa
    47761 gcgcactgat tgaattcctg tcccacaagc cagttccaag agtttctttt tctccttggt
    47821 cttaggcaaa tgacgcagag aaaaatttgt ccatttgtcg tataaactat cgtccatgac
    47881 cgcatcatag accgccgcaa aggtttcata agtcgccata atcatgatac taagagctcc
    47941 atggtaaaca ccactagccc tttacctttc tatcaatttt tctaaaataa gcaaagaaac
    48001 ccagttgact acaactgagt tcatcttcta agcaagagct tctgaaatat ctactgaatt
    48061 cgcctcatgc catagcttct ctagattata atgggcacgc atttcttctg aaaagatatg
    48121 cacgacaaca gcaccgaggt ccagtaagac ccagcctcca gctgcatcgc cttcgacatg
    48181 gctaccttta aagcccgctt gagctacttt ttcacggata ttagctgcga tagcgtccaa
    48241 ctgacggcta ttcattgagc tagtgatgac aaagtagtcc gtcacactag tcaaatcttg
    48301 tacatcaagt gcgaggatat cctccgcacg tttctcatca gccgctttca cgactagttc
    48361 tagtaattct ttttcgttca tttaatcctc tttttagaaa atatgtttgt aatctaggac
    48421 atctgcaaag cgctcctccc aaatgtcctc ataaaattca ttaatcgttt ctgctggaat
    48481 gtcatcccca tcaaaggttg tgacagcacc ttctggaaca ataagctgat agccatattc
    48541 aaaggcaacc ttgacagagg tatccacaca atattctgtc tgcataccac ataaaactaa
    48601 tttttcaatc ccctgcttat ccaagtattc ttttaagcca gtttctttga aaatactgtt
    48661 atacttcttc tgaaagacct tttcagcagg ttttcgattt aaaagcgcag ataactgcca
    48721 atcttctgat gtttgagctt cagagttctc aatatgttga acatagataa tttcaatatt
    48781 cttgcttcta gcctggtttt gtaaataaga aatttttccc aatagacttt ttgtctggaa
    48841 cccagtttct actaaaacat tctgaacatc aatgattata aaagccgtct tcatttagtt
    48901 ctctttcaaa tagtgcacaa aggcgttata ggtttcaagg gtttggggat agatggggaa
    48961 tccctgatga gctagatact ccacggtacg agctgtttcg taggccaccg ccttattgag
    49021 cgatagactt gcaatctcac gcgccacatc tactccagga aaggctcgat tgtgctcgat
    49081 atagtctgcg acgtagatga ccttatctag gtctgtcatt tgaccagcac cgactgtatg
    49141 gacttcaatg gctcgcagga tttctgaatc atgcaaatcc aaatcttcct gaattttgta
    49201 aattccgacc ataccatgcc agacattatt gccccagttt ttgaggtcag ggtctagctg
    49261 gtaacggtca atcaaggcta gaaattcctg atctgacaac tttttagcat agtcatgaag
    49321 aagacctgct agacttgctt tctcaacatc tacaccaaag cgctgagcta attccatagc
    49381 ggcacgctcc acacctagac aatgggttag acgtttctca ggtagaagct ctgccatttt
    49441 ttccaacaag gcctcacggg agcagttgat atagtcttga taggacatca gtagagcccc
    49501 tccttctcga tgtagtctag cactggctga ggtaggagaa agttgggttt ccgaccttgg
    49561 gcaaggaagt cccgcaccat gctggacgag atatccatga gcggtacgtc cacccagata
    49621 actggatagg aagtccctac cttgtagcgt ggacgctgaa cccccacaaa ctgaaccatg
    49681 tcaaccagtt catcaattcg gtaccactta ggcagatagg caaccatgtc ggcaccgatg
    49741 ataaagtaat aatccgtatc tggattcttc tctgtcaaaa tcttcatggt atcgtaggtg
    49801 taggaaatac ccttgcgctc caactcaatg gtttcaatga ctaggccgtc aatcccctca
    49861 attgccaact caagcatctt gagacgatgg tgttcaggga tggtttcctt tttatcaacg
    49921 tgaggaggtt ggtattcagg catgagaaga acttgatcca gtcccaactg ttgccgtact
    49981 tgatccgcaa caatgagatg ggcattgtga acagggttaa aattcccccc taaaatccca
    50041 acttgtttgc gttttttctc cttgatttct ggctccaact ctaccttggt aaagggagtc
    50101 aatagttcga ttgtcatagg ctagtcccct ttatttctct ataaaaacag ttatttggag
    50161 tttcggtctt agatttcttt gactttctta gaaatcttgc gattttcttt cttgctagat
    50221 tgtttaaaca aaatcaagat gcgtcctatt ttttggactg tatccacacc gatttcttct
    50281 tccaaaattt cagctacttc gtggatgttt tcatctgtgt tttgtaagag agtaaccttg
    50341 attaattcac gcgcatcaag agcttgacgg acgctggttt tgatttggtc gttgagtcca
    50401 tttttcccga tttggatgat aggtttgagg gtgtgtgcct ggctgttgag gaaggcacgt
    50461 tgttttgatg ttaatgacat aattttcttc cttgtttttg gatagttatt ttaggtggtg
    50521 agggacggtc ggaactaatt ttccaaagat ttcatctttg aaaaatggat ttcctgacct
    50581 cacaattgag ccttggtctc aattgtgccc cttgccaatc tacagattgg caatcacacc
    50641 acggcaataa ctatcgctat atgagctcac tttgttcgct cagcgatttt gttttaaata
    50701 attgcttttc gtgtgacgac ggcgacgcct tctggtgccc agacggcgac ttttgctatg
    50761 cctgttacac gaatccagcc taggcctgag atgactaggt ctgtcttatc tttaatggta
    50821 aagacatgct ggactagact tgggaaatct tctttttcct tgctatttgg tggtgtcaga
    50881 agagttccca ggtgcttatc gtagaaagca ctagctcctt caagcttgct acgatggagt
    50941 ttgagttcat tatcaaagaa agcagtaaat ccttgctttt ctcctgctat aaagtcaaag
    51001 cgtcccaaac cacctaaaaa tagggtttgc tcaggattaa gctgataggt cttaggcttg
    51061 atttcctttt taggactgac atacttgagg tttttggccg tcaagtagtg agccatctgg
    51121 tggcggtgga taattcccgg cgtatcgtaa atataagatc cgtcgtcaag cggaatctct
    51181 attttgtcca aggttgtccc tgggaagcgt gaagtagtga tgacattctg atcacccgtg
    51241 atttcttgga taatagcatt gattagagtt gattttccaa cgttggtcac accgaccaca
    51301 tagacatcgc ggcccttacg gtagtgttca atcttgtcaa tgacttcctt aatggcatgt
    51361 ttattttgtg ctgaagttag gaccacatcg actggacgaa gaccttcttc atgggcacgt
    51421 ttcatgagcc actggctaat cttaccagac ttaactgact taggaaggat atctttttta
    51481 tttcctacca agaggacatc attgcccgag acgaaacgtg gtaaacctgg gatgacagat
    51541 ccattaaaat caaagatatc aataacattg accactaaag catcactgtc tcccacctcg
    51601 tgcaagagct tgaggaaatc atcgttcgtc aactggacat ctgtgatttc attgtagtgg
    51661 cggagacgga aacagcgttg gcaatagact tcgccagtct ccaaaccttt ttcaagtgcc
    51721 gactgggggg taaaaccaag accagcttta tctgtcgtct gaatggttgc tccacaacca
    51781 atacagagaa tttcttccat agttaaattc cttttttata tgtaatcggt ccgtactttt
    51841 cagtgatttt tctcataaca cgacgctcac gagttcggtt aatctgcgtt ttgattgagt
    51901 catgttggac caagggtttg actaaaattg accgaatccc tgcacggtgg gctgctcgta
    51961 tatctgtcat gagctggtca ccaaccatga ccacttcctt tttgtcatag tggaattcct
    52021 tcatagcacg gtcaatacca aatgtgaagg gcttcaaggc ccagtaaacg taatcaatcc
    52081 caaatttctc aactgctcgt tgaacgcgtt ttttggtgtt atttgacact acgataatgc
    52141 caatacccgc gtcccgaagg tcatgtagcc attgcttcat ctctggcgtt ccatcagggt
    52201 tgttccaagc aatgagggta ttatccaaat cgaccaaaac agccttgatt ccctgcgcct
    52261 gcaggcttgg gactgtcaga tcatagactg cttccacagc aaaatctggt atataatttt
    52321 caatcgccat tctggctcct tttcttaact ttttttcgca attttcctta gtaacaagga
    52381 taaaacaatc cacaaacctg cactagccaa actgatatag agccaagcat ggggctcatc
    52441 tgttaaaggt aagggaacat tcattccgaa aaagcctgtc acgactgcca aaacagctag
    52501 caagactgaa atgatagtca aggttgtcaa attgtcattc agattattgt ttagaatatt
    52561 gttgtaagag gctgaaagct gctgtaaaat ctgagagatt aggtctgtca tggataccag
    52621 ctgatgagcc tcaatcatgg catcatcaaa ctgttctctc tcaatctcat caaaactacg
    52681 atacaaggca tgaccttgaa tatgctctaa caaaatccga ttttgtttgg cagctgccgt
    52741 cagataaacc ataccagtct ccaaatcaga cagggcaaag aggtttttct tggtagttcg
    52801 ctggcgcaag aggtcattga cctcatccct actcttgtcc atctgctcaa tgacaggata
    52861 gtaggcattg ctgataattt ccagactggc aaagagaaac ttataaatcg aaagcgtgtc
    52921 atggctctcc agataacgag tcatctgttc aatgacatag gcgttcttgg tattactaat
    52981 ggtaatcagg cgacgatgct cgacaataaa ggtcatggga aaagtctcat agtaggcctt
    53041 gtcctttttt acgtctagga cattatagat aaaggtaacc gtcccacttt cacggtggta
    53101 gtccatgtgg gcgcgctcgt ttctatccag tgcgtattca atggtttcct tgtccaaacc
    53161 gtaaatttca gaaaggtctt ctagtttatt caactttcct aggtctaggt ctatccaggt
    53221 acaaccattg cccaactgct tttctaaaac catcttttct ccttattaaa ctccctctat
    53281 tgtaccacaa attacttaaa ataacaggtc tagtctgtgc gtgagaaatt gctaacaact
    53341 gtgatatttc ggttagggac aaagtgaagg acttgctcct cgctacgaag ctgtgtcgta
    53401 cgaattccca cactgacaac cttacccgat acagtaatcg gtccatttgt cagaacgacc
    53461 tcatctccca catccagttg acgttcaaag aggatgaaaa agccattgat gacatcagac
    53521 agaaagcctt gggctcccat accaatcgct accccagcaa taccagctcc agccagcaaa
    53581 ctagaaactg gcaaacctaa aatcgacaaa atgcagtaga gtaaaaagaa atatagcgta
    53641 taattaaaca cattttctag taaacgtgag atggtttttt ggcgtccaac atcatgacga
    53701 gacattttta gagaaggttt gacaattctc tgcaccatgg tatgaagcat ttttttagca
    53761 atataaaata caatcaaaag cagtaaaaga gaaatgacct tggttagaat attctcgata
    53821 atggttgtca catctagctt ttcaatataa gcctgaataa atttttgcat ataaaactcc
    53881 tttatttcat tataccatat tttcatcagt ctcattctcc ataaatattc aaaaaataga
    53941 cagaaaattc ttgattttgt ctatcattta tactataatc ataatcacta cacaaaaagg
    54001 agattattat gaaaagaatc attcatgctt ggaataaggc aagccttgtc aagcgtatct
    54061 tgattggtat gctttgtggg gaatcctagg actgaccctt cctaatctct cagggattgg
    54121 gctactcggg gatttattcg ttggaggtct aaaagctgtt gctcctatcc tagtctttgc
    54181 cctcgttgcc aatgcccttt cccaacatca aaagggacaa gatagcaata tgaaaactgt
    54241 tgttttcttg tatatcctat gatagggact ttcgccgctg ctcttgtagc tgtactagca
    54301 agtttcatcg tccctattga aattacccta aatagtgcca atactgaaat tgcaccacca
    54361 gatggggttg ggcagatcct cagcaacctc ttgctcaaac tggttgacag cccagtcaac
    54421 gcgccctgtt tactgctaac tatattggaa tcttatcttg ggcagtcatt tttggaatcg
    54481 ctatgagaga agccagtaaa aatagtaaag aattgctaaa aactatcgct gacgtgactt
    54541 ctaaaattgt cgaatggatc atcaatctgg ctccatttgg aatccttggt cttgttttta
    54601 aaaccatttc tgacaaggga gtcggaagcc ttgccaacta cggtatttta ttggttccat
    54661 tagtaacgac tatgcttttt gttgcccctg tggtcaaccc tttgattgcc ttcttcttta
    54721 tgagacgcaa tccttactct ctagtttgga actgcctccg tgtcagcggt gtgacagcct
    54781 ttttcactcg tagttctgcg actaacattc ctgtcaacat gaaactctgc catgaccttg
    54841 gactcaaccc agatacctat tctgtttcta tcccactcgg ttctactatc aatatggctg
    54901 gagtagcgat taccattaac cttttgaccc ttgctgcagt taacactctt ggaattcctg
    54961 ttgactttgc cacagccttt gtcctcagtg tggtagcagc tatctcagcc tgtggtgctt
    55021 caggtattgc cggaggttcc ctccttctta tcccagttgc ttgtagcctt ttcggtattt
    55081 ctaacgatat tgccatacaa gttgttgggg ttggttttgt gattggtgtc atccaagact
    55141 catgtgaaac agcccttaat tcttctacag atgtcctctt taccgccgtt gccgaatacg
    55201 cagcaacccg taaaaaataa ctcatcaagg caagcctgct catgtcttgt cttttacgct
    55261 tttattctaa cttattagga aattcttatg tctattagcc aacgtacgac caagctcatc
    55321 ttagctacct gtcttgcctg cctgcttgct tattttctca atctttcgtc agcagtttcg
    55381 gctggaatta tcgctctctt gagcctatct gatacgcgta gaagtacttt aaaactggct
    55441 cgcaatcgtc ttttttctat gcttctagct ctggctatcg gtgttctagc ttttcacttg
    55501 agcggatttc atatctggag tctcggcctc tatctggcct tctacgttcc tttagcctac
    55561 aagatgggct gggaaattgg catcacacca agcacggttt tggttagcca tctcttggtt
    55621 caagagtcaa cctctccaga cctcctagtc aacgaattcc ttctctttgc tattggtaca
    55681 ggatttgcct tgcttgttaa tctctatatg ccttcacgag aagaggaaat ccagcactac
    55741 cacacgctgg tggaagaaaa gttaaaagat atcctccagc gcttcaaata ctatttatcc
    55801 agaggagacg gacgcaaccg agcacagctg gtagcagaat tagacacgct tttgaaagaa
    55861 gccctcagac tggtctattt ggatcactct gaccacctct ttcaccagac cgactaccat
    55921 atccactact ttgagatgag acagcgacaa agtcgtatcc tgagaaacat ggcccaacag
    55981 attaacactt gtcaccttgc cgccagtgaa agcctgatct tagcgcaact cttttcaaaa
    56041 attgcaggtc aactgagcca gaccaatcct gcttctgatt tgctagatga aattgaacgt
    56101 tatctggaag tcttccggaa ccgcagtctg cccaaaacaa gagaagagtt tgaaacccgc
    56161 gccacccttc ttcaactcct acgtgaagcc aaaaccttca tccaagtaaa agttgatttt
    56221 taccaaaaat atagacagta aaaaataaaa ccactaaaaa acagagcttg ccagcattct
    56281 tgaaaagtag tataattatt gcatgcgcaa tcatcttgtg acgcagagac tgcccgttaa
    56341 tggactttct tctatttatg actctaaaaa gaaaggagac actatgacct atttggaaaa
    56401 atggtttgac ttcaatcggc gtcagaaaga aattgaaagt ctcttggaag agaccattgc
    56461 ccaacagagc gagcaaagtc tgaccttgaa agagttctac ctgctctact atctggactt
    56521 ggctgaagaa aaatctctac gccagattga cctgccagat aagcttcatc tgagcccgag
    56581 cgcagtttct aggatggtgg ctcgcttgga agctaaaaat tgcggtctac tcagtcgcat
    56641 gtgttgccat caagatagac gctctagttt tatctgcctg acaaatgatg ggcaaaagac
    56701 actggcctcg ctacaaaaga ctgtcgaaga aagcttggaa gcaggtttgg atttcttgat
    56761 ttaagatgat tttaaaaaaa gttgcgtgcg caatcatttt tcttgacatt ctcttttata
    56821 aggagtaaaa taaagttagc attaaacaaa ggagttttaa acatgattga aattacctat
    56881 atagatgcca gcaagaacga aagaactgtg actttcgagt cttatgaaga ctttgaacgt
    56941 tcgcaacaag cttgccttat cggcgtcgca gactactacc ctgtccaaaa attaacttac
    57001 aagggtcata atttggacta ccatgggact tatggagata tcttcttcta tctcatgaaa
    57061 caagatttaa gccaatataa ctaaaaaaag gagaaataca atggcaaaag caattacaga
    57121 tgcaacattc gaacaagaaa caaaagacgg tttggtctta gtagacttct gggcaacttg
    57181 gtgtggtcca tgtcgtatgc aaggtccaat cttggacaaa ttgtctgaag aactttcaga
    57241 agatgtcttg aaaatcgtta aaatggacgt tgatgaaaat ccaaacacag ctcgtgcttt
    57301 tggaatcatg tctattccaa ctcttctctt caaaaaagac ggccaagttg tcaaacaagt
    57361 tgcaggtgtt cacacagcag aacaaatcaa ggccatcatt gctgaattga gctaatcaaa
    57421 cgagagacca agtactttct ttggtctctt ttttcttgcc ctttgccatt tttcaaaaaa
    57481 tatgctagac tatagcagat tgaaactaga atagtacacc tctacttcta aaacattgtt
    57541 agaaatcgat ttgactgtcc tgatcgattt gtcctgttct tatttcattt tactatataa
    57601 gtagaatatt acgatgtttg ggatatgcca tcgctaaaaa aactcgattt ggtatttttt
    57661 agctccctaa tgggagcttt ttgcatgctc tgaacatttc ccattttgga aggagtacta
    57721 tgaaacgtca atcagccttg gtcgtcttta gtggtggtca agattcaaca acctgccttt
    57781 tctgggccat gcaacactat gaaacagtcg aagctgtcac ctttgcctac ggccaacgtc
    57841 atcacctcga aattcaaatt aatagagaaa tcgctaagga acagggcatt cgtcaccata
    57901 tcctcgatat gtctctgctg ggacaaatca ctgctcagcc agactttgcg acgattcata
    57961 tttcctacat tcctgacaaa ctctgtgtcg agtcaaaatc cctcaaacta tatctattta
    58021 gctaccgaaa ccacggagat ttccacgaaa actgtatcaa caccatcggg aaagacttgg
    58081 tcaacttgct agaccctcgc tatttagaag tctgggggaa attcactccg cgcggtggca
    58141 tttcaatcaa cccctactac aactacggta agcaaggaac taagtatgag ggcttggcag
    58201 aacaacgcct cttccaacac gacctttatc cagagaaaat tgacaaccgc taaagtcata
    58261 ctcaatgaaa atcaaagagc aaactaggaa gttagccgca ggttactcaa aacactgttt
    58321 tgaggttgtg gatagaactg acgaagtcag taaccatacc tacggcaagg tgaagctgac
    58381 gtggtttgaa gagattttcg aagagtatca tacgaaaaag ccctgttcct caagatgagg
    58441 agcaaggctt tttgagtttc aattattctt ctgttccaag gaaatttttt gcaacaaggg
    58501 ctgcaagaac tccaccagcg attggtgcaa ggatgaaaat ccaaacttgt tgaagggctg
    58561 cgccgcccac caagacagct ggtgccaagc tacgagctgg gtttactgaa agtccagtaa
    58621 tcttcaatcc gatcagaaaa agacttccaa ctcactgaag ctgggaagtc ttatttccaa
    58681 agaaaattac acattgatac ggatgaattg aaaaagaaaa aacggaaatt ttgccctaaa
    58741 tgtttggatt ggagcgaacg taaaaatcat gtcggcggag ctctcggcaa tgctctttta
    58801 gaatttttca gtgaaaaaca attggtcatc cctgcacaaa cgccacgttc acttaccatt
    58861 acagagaagg gcaaagaatt cctcgaaaag gaatggggga tttaaacatg gacttttaag
    58921 caaataactg tccataccca ttctataaca acctctctat ccccttactc ccccaactca
    58981 gcactgtagg cgataatctg gtcaactgtg tcagacaaga attggatggt atcacggagt
    59041 ggtttgtctg ttgaaatatc agcaccgata atcatggctg actcaagtgg tgtcttgcta
    59101 ccacctgatt tgaggagatt gagccagtct tcagctccag tttctgagtt tttaagatga
    59161 aggtaaccag ctgtcgagat gacaagacca gcagagtaag tgtaactata caagcccata
    59221 tagtagtgag cttggcgcat ccaagtcaaa gctgcatcgt cgtcgatttc aatggcatcg
    59281 ccccaaaaat ctgtcaaaac ttccttcata atgctgttga gcttgcttgc tccaaaggtc
    59341 tccccttctt caatcaatgt atacacctta cgctggaagg cggcttccaa gaggtgggtg
    59401 atgaaattat ggaagtaggt gtctgtcaag cgatgagcca gagcgaagcg tttttgacgt
    59461 gggtcattag actggttctc taagtaatca ctgagtagca attcattgaa ggttgacggt
    59521 gcttcaacat aataggtcga catgtgggcg ttgaaatagc tttggtgatt gtctgaaaag
    59581 atgaattgac cagaatgccc gatttcatga atcaaggtat agacatcgct caaacggcct
    59641 gtccagctca tgagtacata agggtgtacg cgatatgggt ccgccgcata accaccggaa
    59701 tccttgccac tgttagcagc aaaatccacc cagcgctctt cttggtaacg agcaacttcc
    59761 tgacaatatt cttgccccaa aggttctacc gacttcatga ccaaatcata ggcatcgtca
    59821 atagtcactt caggattcag ggcgctgtcc aagtccaatt tccagtctgc aaaggtcatc
    59881 ttttcaagac catttacctt ggcaacatgc ttgaggtatc tctgagcgac tggtgcaaag
    59941 tccttcatga tgaggtcaat ctggcggtca aacatgacac ggtccacttc ttgttcagct
    60001 agaagatagt caaagacaga gtcgtatccc ttcatatcag ccaagagttt ttcagacttg
    60061 acctgagcca gataggctgc tgcagccgta ttttggtgct tacgaagtcc ctctgagaag
    60121 gaacggaagg atttctcacg aacctcagca tcctcgtgat tttggtagaa attctcatag
    60181 gtgacaaagc tgtttttata ggtcttgcca tgggcttcaa agtcagccat ttcaaaatcc
    60241 ccagctcgca tcttagtata aatgtcctgc ggactataga aaacttcacc gagatttgtc
    60301 aaggccttct ccacatctgc ccctaagtag tgggcttttt tgattttagc ctgacgaatg
    60361 gcagctgtta aatgtggcaa tttacccaaa cggtccaaga cttcctcgtc tgctgccacc
    60421 aaggcatcgt caaagaaggt caaggctacg ctggcagctg tttcaaattc catcccagct
    60481 tgggcaatat tggcaaattc gtcattgcta tagtccgtcg tctgaggcat aaaagcatag
    60541 ttgccaatat ggctcatctg aatgtagatc tgttccaatt ccgcaaaggc cttctcgaaa
    60601 tcctcaaaag tgtgaagatt gcccttgtaa tcacggctaa actggttgat gtcttcgcga
    60661 gctttctcga ttgcacgcaa gaaatcctca cggtcttggt atagggctgt taagtcccag
    60721 agttccttct ctggaaattc tgaacggtgt ttttgttcca ttttcttcct cttatttctc
    60781 taattctact aaaacactaa gggctgataa agcgtaaagc ggtgctgttt ctgctcgcaa
    60841 aatacgagga cctaggcctg ccaaaacggc tcccttagct tcaaaacttt cgatttctgc
    60901 aggtgagaga ccgccttctg gaccaaagat aaagagcagt ttggctcctg tttcaagacc
    60961 agtgactgct tgcagaagcg cagcggcttc tccttcttta gctgattctt cataggctac
    61021 tatgatagag tcaaactggt ccagctgagc tagaaaatct gcttttttct cgaaaagttt
    61081 aatacttggt acaatattac gcttgctttg ctcggctgct ccaagggcaa ttttttctag
    61141 tttttcaact tttttaccca atttcttgcc atcccacttg gcaactgacc agtctgcagg
    61201 aaaggcccag atttggctag cacccagttc ggttactttt tgagcgatga actccagctt
    61261 gtctcccttg ggaaatccag atgcgatggt cacttggact ggtagttcca cattgtcatt
    61321 taattcttgg accaactcaa actgacgatt ttccatatcc agcacgcgcg ccaagcgctt
    61381 gatgccatca tcaaagacta aggtaacctc atcctcttct ttcaagcgca taacctgaaa
    61441 catatgctta ctggtttcct tgttctcgat agtgacagga gagatagcac tgccttttac
    61501 aaaatactgc tgcatgctag cctccaatca caccagagat atccttggtt ttcttaaaga
    61561 cacaggcatt ccattcccct tgaaccatgt gagtttcgag gaaaaatcca gctgattcag
    61621 ccaactcgcg caccatgtcc cacttatcct tgataatgcc actcatgatc aggtaacctt
    61681 catccttaac caagcgataa gcatcctctg tcagatgaat gaggatatcc gccaagatgt
    61741 ttgccacgat gacatctgct tcaatctcca cgcctttaag caaatcgcct gccactacat
    61801 ggatattttc cattccaggg ttgagctcaa tattttcctg agccacacga actgccacat
    61861 catccaggtc ataggcgaaa atttctttag cccccagaag cgagctggca atagagagaa
    61921 cccctgaacc agttcccaca tctagcaccg tttcgccacc acgaagaacc tgttccaagg
    61981 caaaaatgct catcttggta gttgggtggg ttcccgtacc aaaagccatg ccaggatcca
    62041 gcttgataat tttttctccc gcagtcgcct catagtctgt ccaagaggga acgatggtca
    62101 aatcatgagt gatgcgagca ggttcatagt atttcttcca gttgtctgcc cagtcttcct
    62161 cagccaaggc agtcgtacct atttttaact ctcccaaatc cataaaatct gtcaattctg
    62221 ctagacgagc ctgcaaatcc gcctcaacca ctgtcacatc caccgtgtca gggtagtagg
    62281 ctgtcactac gatttcttct tgctgctcca cctctgggaa aatctcgcca aagcggtcaa
    62341 catttcccac atagtccata ctgtcttcga ttgcgactcc ttgcgctccc agctcaatca
    62401 agagattgga aaccaactcc tctccctcac gcttcactgt aacttttaac tcttgccatg
    62461 tttccattat gtattttgct tacctttcca tcagattaaa aatagtcttt ccaataacaa
    62521 caataaggtt atctcttaat ttcttctcta actctaaatc aattccacta ttttgtatga
    62581 atttgtcttc tacatttcta tataagcgtt ttactctagt ggggtttcct gcatcaaaat
    62641 gaggtacata atggtaaatt atgacaggaa cagaacttct gattgattgt tcagcaatct
    62701 gattccagat atacccatca gtctctccca aagaaacccc ataaagaatt atgatgtcag
    62761 aattttttat aatatcgcta tttttaacat ccatattttc tctatagttc ttcaaaacta
    62821 aattttttat taaggattct ttttgttctt catcaaaact atttgaaatc tgtgtctcgt
    62881 cactaactcc taaaactgtg caaatatcta ccgttccatg tgcatgaatt ggaggcgcaa
    62941 tgtgaaattt ataattatta gttcgtaatt gattgctaaa tgattttgct gactcattat
    63001 ataatttatc gattacattt gtgtaattaa gagttaatat attcacgata tcgtgttgat
    63061 atagattttc attatatttt ttatcgatag caggttgatt aattacaggg agagaggtcc
    63121 tcatattatc tagagtgctt ataaagtcaa ttgcattacc tttctcaaga tttttttctt
    63181 gttgtatgcg tagatattct cttaaatcat caactatttc actaaaatca tctataaatt
    63241 tctcctttat ttctatactt gaggagatga ggtcgttatc ttttgttaat ttaccaattg
    63301 acaattcaaa
//
LOCUS       DAVLRT010000004        48672 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLRT010000004 DAVLRT010000000
VERSION     DAVLRT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.004"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(266..1252)
                     /locus_tag="VXM85_000226"
     CDS             complement(266..1252)
                     /locus_tag="VXM85_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1344862.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1262..2062)
                     /locus_tag="VXM85_000227"
     CDS             complement(1262..2062)
                     /locus_tag="VXM85_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1344863.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2315..3157)
                     /locus_tag="VXM85_000228"
     CDS             complement(2315..3157)
                     /locus_tag="VXM85_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1344864.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(3159..4451)
                     /locus_tag="VXM85_000229"
     CDS             complement(3159..4451)
                     /locus_tag="VXM85_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1344865.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4562..5833)
                     /locus_tag="VXM85_000230"
     CDS             complement(4562..5833)
                     /locus_tag="VXM85_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1344866.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6382..7899
                     /gene="malQ"
                     /locus_tag="VXM85_000231"
     CDS             6382..7899
                     /gene="malQ"
                     /locus_tag="VXM85_000231"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1344867.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7925..10183
                     /gene="glgP"
                     /locus_tag="VXM85_000232"
     CDS             7925..10183
                     /gene="glgP"
                     /locus_tag="VXM85_000232"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1344868.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYKVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11228..11800)
                     /locus_tag="VXM85_000233"
     CDS             complement(11228..11800)
                     /locus_tag="VXM85_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344869.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLTQLWDKNDAIPNNGRINF"
     gene            complement(12425..13273)
                     /locus_tag="VXM85_000234"
     CDS             complement(12425..13273)
                     /locus_tag="VXM85_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1344870.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13425..13703
                     /locus_tag="VXM85_000235"
     CDS             13425..13703
                     /locus_tag="VXM85_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344871.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13705..15768
                     /locus_tag="VXM85_000236"
     CDS             13705..15768
                     /locus_tag="VXM85_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1344872.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15846..17102)
                     /locus_tag="VXM85_000237"
     CDS             complement(15846..17102)
                     /locus_tag="VXM85_000237"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1344873.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17244..19709
                     /locus_tag="VXM85_000238"
     CDS             17244..19709
                     /locus_tag="VXM85_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1344874.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLCFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLATADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19774..20685
                     /locus_tag="VXM85_000239"
     CDS             19774..20685
                     /locus_tag="VXM85_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1344875.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20915..21613
                     /gene="dapD"
                     /locus_tag="VXM85_000240"
     CDS             20915..21613
                     /gene="dapD"
                     /locus_tag="VXM85_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1344876.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21681..22811
                     /locus_tag="VXM85_000241"
     CDS             21681..22811
                     /locus_tag="VXM85_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1344877.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22823..23362
                     /locus_tag="VXM85_000242"
     CDS             22823..23362
                     /locus_tag="VXM85_000242"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1344878.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVAYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23346..24023
                     /locus_tag="VXM85_000243"
     CDS             23346..24023
                     /locus_tag="VXM85_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1344879.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(24090..24989)
                     /gene="galU"
                     /locus_tag="VXM85_000244"
     CDS             complement(24090..24989)
                     /gene="galU"
                     /locus_tag="VXM85_000244"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1344880.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYKRTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(25011..26027)
                     /locus_tag="VXM85_000245"
     CDS             complement(25011..26027)
                     /locus_tag="VXM85_000245"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1344881.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            26239..26679
                     /locus_tag="VXM85_000246"
     CDS             26239..26679
                     /locus_tag="VXM85_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1344882.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            complement(26802..27218)
                     /locus_tag="VXM85_000247"
     CDS             complement(26802..27218)
                     /locus_tag="VXM85_000247"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1344883.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(27286..27936)
                     /gene="phoU"
                     /locus_tag="VXM85_000248"
     CDS             complement(27286..27936)
                     /gene="phoU"
                     /locus_tag="VXM85_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1344884.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(27951..28703)
                     /gene="pstB"
                     /locus_tag="VXM85_000249"
     CDS             complement(27951..28703)
                     /gene="pstB"
                     /locus_tag="VXM85_000249"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1344885.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(28705..29520)
                     /gene="pstA"
                     /locus_tag="VXM85_000250"
     CDS             complement(28705..29520)
                     /gene="pstA"
                     /locus_tag="VXM85_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1344886.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDIMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(29513..30376)
                     /gene="pstC"
                     /locus_tag="VXM85_000251"
     CDS             complement(29513..30376)
                     /gene="pstC"
                     /locus_tag="VXM85_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1344887.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(30494..31369)
                     /locus_tag="VXM85_000252"
     CDS             complement(30494..31369)
                     /locus_tag="VXM85_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1344888.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(31536..32867)
                     /locus_tag="VXM85_000253"
     CDS             complement(31536..32867)
                     /locus_tag="VXM85_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1344889.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(32868..33575)
                     /locus_tag="VXM85_000254"
     CDS             complement(32868..33575)
                     /locus_tag="VXM85_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1344890.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(33659..34144)
                     /locus_tag="VXM85_000255"
     CDS             complement(33659..34144)
                     /locus_tag="VXM85_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1344891.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            34130..34669
                     /locus_tag="VXM85_000256"
                     /pseudo
     CDS             34130..34669
                     /locus_tag="VXM85_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(34984..36675)
                     /locus_tag="VXM85_000257"
     CDS             complement(34984..36675)
                     /locus_tag="VXM85_000257"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1344892.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            36811..37257
                     /gene="argR"
                     /locus_tag="VXM85_000258"
     CDS             36811..37257
                     /gene="argR"
                     /locus_tag="VXM85_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1344893.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            37308..39842
                     /gene="mutS"
                     /locus_tag="VXM85_000259"
     CDS             37308..39842
                     /gene="mutS"
                     /locus_tag="VXM85_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1344894.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            39989..41683
                     /gene="patA"
                     /locus_tag="VXM85_000260"
     CDS             39989..41683
                     /gene="patA"
                     /locus_tag="VXM85_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1344895.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEVIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(42013..42276)
                     /locus_tag="VXM85_000261"
                     /pseudo
     CDS             complement(42013..42276)
                     /locus_tag="VXM85_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            42470..44236
                     /gene="patB"
                     /locus_tag="VXM85_000262"
     CDS             42470..44236
                     /gene="patB"
                     /locus_tag="VXM85_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1344896.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTENKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            44375..45064
                     /locus_tag="VXM85_000263"
     CDS             44375..45064
                     /locus_tag="VXM85_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1344897.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQIGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            45068..45247
                     /locus_tag="VXM85_000264"
     CDS             45068..45247
                     /locus_tag="VXM85_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344898.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            45234..46583
                     /locus_tag="VXM85_000265"
     CDS             45234..46583
                     /locus_tag="VXM85_000265"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1344899.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            46707..48167
                     /locus_tag="VXM85_000266"
     CDS             46707..48167
                     /locus_tag="VXM85_000266"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1344900.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYSRHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            48181..48615
                     /locus_tag="VXM85_000267"
     CDS             48181..48615
                     /locus_tag="VXM85_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1344901.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        14342 a         9256 c        10563 g        14511 t
ORIGIN      
        1 cgtcagtttc atctacaacc tcaaaaccat gttttgagct gacttcgtca gttctatcca
       61 caacctcaaa acagtgtttt gagcaacctg tggctagctt cctagtttgc actttgattt
      121 tcattgagta taaaaagatc ttgggtaaca actcaatttc agtagaaaag gaagtaaatc
      181 ttcccacaat aaaacgcata gtatcaagtt ttttcaatac ctgatactat gcgtttttgc
      241 ttttaacgac tttttgccca gtctcttatt tttcgataat tttgtgggcg atcaattgac
      301 ggtaacaaat ttgtttattg tttttattat cattaataat ctggagaata gtttcaaggg
      361 aaacacgacc aagctctaaa ctattgatat cgacataggc tgccaagttg agcttgggat
      421 taaccgagtc aaagctgaga acagggacat ccagctggtg tttggcaata tagttacaaa
      481 caccttcagc taggaggcta tcggttgtga tgatagcatc aatttgtgga tcgtgcttga
      541 ataatcgctt gctaaattta tagccctttt cttccagaaa ctcgtcggca aagtagatgc
      601 gattgttgtc agtggtaagt ttgtaatgtt taagcgcctg ttcatagcct gttaaacggt
      661 ctttggtcac gaagagcttt ttacttcctc cgataaaggc aatgcgtttg cagccttttt
      721 tgatgaaata ttcagtcgca tcaaaaccag cttgaacatt gtcgttgtcg acaagtggga
      781 tgaaaggaga tagagattta cctaagataa ggaaggggaa ctgttcttct gcgacgagtt
      841 ttacgagagg gtcttcttct tgggcataga gaaagattag cccatctaca cgcttgccgt
      901 agaccatttg tgaaatagcg ttgagacgct ccttctcatc tttccctgtt gctatctgaa
      961 tggcatagtg gttttcagat gcgacttgag agatgccacg tagaaccgat ggaaagaaag
     1021 gattctggta gaaggcgtct gagtcatcag gaagaactaa tccgataacc tgagtatagc
     1081 tgcttaccaa gctacgagcg ttgaggtttg ggtggtagtt gagttccttc atagctttgc
     1141 gaacgcgttt ttttgtttcg tcgctaatgg ttgatttatt ttgaataaca cgggttacgg
     1201 ttgaaggcga aacaccagca gccttggcca cgtctttaat cgtaacgggc ataaaaatct
     1261 cctatttatg gtaatttgga tcacggaaat gtgttttata aaagatagtg accagataca
     1321 gaacaaaaag aatattttgt acagtaatca gggttgtcat attttggcca aataatccca
     1381 aaataagtgt aatcagagtc ggcaatccta aacagttcaa gataaagtga tagcactctt
     1441 taaaggttct aaatgaaaag aggcgtgatt tcttagtgat ataaaggaga agactagctc
     1501 ctagagagac gataaagaaa ttcaaaccaa agaggaagct cgcaccgaga actaggaaga
     1561 ggctgatata gacacgattt tgttggtacc agtctttaga aattgcttgg gtcaagctgt
     1621 ctttgctttt gaaactctca gtctgaatgg ctcggtaaga gatgcgggtc agttccttgc
     1681 tttccttgct gatgactagc tcatttgtat cgaaatgcag ttgcaagtcc ttaggtaatt
     1741 ccttgatttg acttggacca atcacaacag aaggggcttg actagctgtt ccagtataag
     1801 ttaatgtgcc atcgacaatt gtagcatgtt cagagagatc ctggacaacc tcatttgtca
     1861 gaggttcata gacattatcg ataaaagttt ctagcggata ggtctcctgg gagctgtttt
     1921 ggatagcaat gggtaccata gacaagctga taaggaagat actggtaaag agaagttgaa
     1981 accagttgag ctcgaaacgt ttggacaggg gcttacgaaa cccccaaata cttgaaaaat
     2041 aggaaaatgg atatggaagc ataggtatct ttctaaaagg tgttttctat atgagtatta
     2101 ttatatagag aaatgctctt tatttcaagt ctaggagaaa aattgcttgc tacaaggata
     2161 ttatttacag tgacaattgc taataatcaa tgaaaatcaa agtgcaaact agccacaggt
     2221 tgctcaaagc acaactttga ggttggagat aaagctgacg tggtttgaag agattttcga
     2281 agagtataaa atgaaaaagg gtggcgggga taaattatcc cttgtcgcca ccacttgtaa
     2341 gtcctgaaac aaagttcttt tgtaggaaga agaagagaat acagattgga agggcgatga
     2401 ggatagcacc tgctgagaag taggcaatct tcaagttttt cgcattgtta acgaaggttt
     2461 ggagacctac ggcaacagta aagtattctt tctcacgaag caagaaacta gagaggatgt
     2521 agtccccgaa aggtcccatg aaggcccaga gagcttgtac ggcaaccatt gggcgaacaa
     2581 gtggtagaac aatttgccag aagcggcgga agtgtcctgc accgtctagt tttgcagatt
     2641 cgtctaaaga cattggcact gtatcgaagt agcctttcat gagccaagca ttcatcggga
     2701 taccaccacc aacgtagagg aagatgagga accagttgtg gttaagggcg ttcaacataa
     2761 gcgccataac gaagaaggct gtcaaagcgg ccatagttgg caccatttgg atgatcaaga
     2821 agaagaccaa actttgttta cgagccaaga agttgtaacg gctgtaggca taaccagcaa
     2881 gtacgatgat acttgtttga acagccatgg taattaaggc gataatcaaa gtgttgaggt
     2941 accaagtacc gtacaaggtt tcagtgaaga ggcctttaaa gttatcaaaa ttgaggtcga
     3001 tattagtatc tagtttaaag gctgagacgt tacctgcttt aaaggctgac ataatggtaa
     3061 tcaacagtgg atagataatt acaattgata gaccaatcag gtaaaggtaa gtaaggcttt
     3121 gagtcagtct acgtttgagt ttaattgagt tattcatctt agacgtcctc catatcaaat
     3181 gcgtgtagtt tcttgaatgc gatcatagag attgagatga caatgataga gataatcaag
     3241 gtaacagctg ccgccattga gtattgagga gatgtacctg ttgtcaaacg gtagatccat
     3301 gagatcaaga tatcggttga accagctcca cctccgacac taccaggtcc tccaccattg
     3361 aagaggtaca tgatagagaa gttgttaaag ttgaaggtgt attggctaat caaagtaggt
     3421 gccgcaacag ccaaaatcat tgggaaagtg atgttgcgga atttttgcca agcgttggca
     3481 ccgtcaatat aagctgcttc gtaaaggtcg ttaggaatag attgcaagat acccaaagtc
     3541 aaaacgtaga tgtatgggaa tccgagccaa ccttgcatca taatcaaggc aatcttagtc
     3601 caagttgggt ctgttttcca aggaataaga gctccatcaa ggaaaggaag gaatttagcc
     3661 aagattggca atacttgagt gttgatagca ccgacactat cgttaaacat gtttgagaat
     3721 gtcaagatag tgatgaaggc tgggacagcc caaggaagaa ggaaaataac accaaagata
     3781 cgttttcctt tgataaatgg ttggttggca atgatagctg tgaagatacc aattacgatt
     3841 tgtaaagtag aagctgccaa agcccaaatg atagtccaag aaagaacaga accaaaggca
     3901 gaacggaagg tactcaagct ccaaatgttt gtaaagttgg tcaaaccaac ccagtccaac
     3961 aacttgtttg gtggcaagtg ttggaagtcg tagttggtaa aggcgatcat caaggttacg
     4021 ataactggga agataatcgc gaaggtcatg gcaacataag atggaatgat caagaggtaa
     4081 gggaagccat tttcataaat ccctttgatc atgtctttga gtgtgcgtgg aactggaatt
     4141 ccattgttaa tgcgttttgc aatcgtatgt gcatctttga tatttgagaa ataaaagagt
     4201 acataaacga ttacaaagat tagatggaag gcaccacgaa tcagcataaa gagggaatta
     4261 tcacgacctg gtttgtcacc aagagtgatg aggttgctca attcaggggt tgcaagtgct
     4321 aggaagtaaa ggacaaatac gatggttaca ccaaggaaga taaaaccttt ggctttttgt
     4381 ttattgtaaa tctgtcctaa cccaggaatg atagacagca gggctgcttt actaggttgt
     4441 tgcttttcca tattaactcc tttcatagga tactactttt ttataagaaa gctaaattat
     4501 ttgtgtctct attgataaat tcaaaggggg atttgatttc cacccccctt gaacaaattt
     4561 tttattcacc aaatttttgt ttgattgttt ctttgatcaa tgttacagca tcgttagcag
     4621 ctgttttagc atctttttga ccacttacag catcaaagag catatttttc gctggatccc
     4681 aaactgcaga catttgagag atgtttggca gtggttgagt gttcttgaac tgtttgataa
     4741 cagctgttgt caactcatcg tttttacctt cagcgtatga acgagcctca gtattagctg
     4801 ggatttcgtt agtcttatca tataatactt tttgttgttc agttgcaaca aggaagtcta
     4861 caaatttttg agaagcttca aggttcttaa cggcttgagg aatgacccaa gctttaccac
     4921 caccgaatgc agcatattct tttccatttg gaagagttgg gatagttgca actccgtagt
     4981 ttactttagc atctttaaag gcttgagctt tccaaggtcc gtcgatgata gcagctgttt
     5041 taccttcttg gaattgagtt tggattaagt ttccagcacc ttctgtatct tgcatacctt
     5101 taggccattt ttcgtaccaa gatttagcgt agttgatacc tgcgatagaa ccgtcgtttg
     5161 caagaccgat gtctttagcg tctttaccgt tttggccaaa gacgtaagca ccgttaccgg
     5221 caagaagtcc atatgtatag tagaagtttg tccagtcagc taggaaggca gtagttttac
     5281 catcttcacc agcgaatgcg tatttgctat ctttagcaag gttttccaag tcagcaaatg
     5341 tttttggagc atctttcacc aagtctttgt tgtagtacat aacaagtgac tcgataacgg
     5401 caggagcacc gtaaacttta ccattagcag ctgttacaag agatttagtt gtgtcgtctg
     5461 ttttagcacc atcgctcaat ttcacttctg aaagttgtcc gtcagaacca aggctaccta
     5521 cacggtcgta tggagccatc ataacatcag ggacattacc agattggttg tcaagagaaa
     5581 gtttatcaag acctcctaga gcatcaccag ttttaagagt gacttttact ccagcttctt
     5641 tttcataagc tttagcaacc tcttcaatat agctcttata tccctcgtct acatatacag
     5701 tgagttcttt gacttcagat gaaccagaat cagcaggctt atcagcagtt ttgcttccgc
     5761 aagctaccaa aagcaagcta gcaagtgtag cagttccaag caccgcagtg ctcttcataa
     5821 atttagatga catagtgtat tcctcctaaa gaatagcaag ttttattgat aaggaaacgc
     5881 aaacgttttc ctttatgagc ttagtatagc acaaatagaa aacggttgca agtatttttt
     5941 gtaaaaattt taaaaaattt ttaagcttga tttgatagta taattttgca ttttaataga
     6001 agaaaagtat gaaatagata agaatacagt atttttaata aaaatatatg gaatctgtct
     6061 aaagagggaa atcaggaggg gtacctccct gtttctaatc tatatctatt ttctatagac
     6121 gtttgtgctt tgttgcatat aaattcaagc tccctaagga ggttttgtac tttgtatact
     6181 gtctcgtgag agtcaaactt taggattaga tagttttaag ttatctttgc taaaatgagt
     6241 tagtctcctt aaaaataatc aaaaaagttt tttaaaaacg cttgcaatta tgcttgaaaa
     6301 ggagtatact tataagtaac gcaaacgttt gcgtctgaaa aatacgcaac gttccattat
     6361 tttaacacac gaggtgctat tatgaaaaaa cgtcaaagtg gtgtgttgat gcacatctct
     6421 tctcttccag gagcttacgg aatcggatca tttggtcaaa gtgcttacga cttcgttgat
     6481 ttcttggtcc gtacaaaaca acgttactgg caaatccttc cattaggagc aactagttac
     6541 ggggattctc cttaccaatc tttctcagcc ttcgcaggaa acactcattt tatcgattta
     6601 gatatcttgg tggagcaagg tttgttggaa gcaagtgacc ttgaaggagt tgactttggt
     6661 agcgatgcgt ctgaagttga ctatgctaaa atctactatg cacgtcgtcc tcttttagaa
     6721 aaagcggtga aacgtttctt tgaagtcgga gatgttaaag attttgagaa atttgctcaa
     6781 gacaaccaat catggcttga gctctttgct gagtatatgg ctatcaaaga gtattttgac
     6841 aatcttgctt ggactgaatg gccagatgca gatgctcgtg ctcgtaaagc ttcagcactt
     6901 gaaagctatc gtgagcaatt ggcagacaag ttggtttacc accgtgtgac tcaatacttc
     6961 ttcttccaac aatggttgaa attgaaagct tacgctaacg acaaccacat cgaaatcgtt
     7021 ggggacatgc caatctacgt agcggaagat tcaagtgata tgtgggcaaa tccacatctc
     7081 ttcaaaacag atgtcaatgg taaggctact tgtatcgcag gatgcccacc agatgagttt
     7141 tctgtaactg gtcagctttg gggtaatcca atctatgact gggaagcaat ggacaaagac
     7201 ggctacaaat ggtggattga acgcttgcgt gaaagcttca aaatctacga tatcgttcgt
     7261 atcgaccact tccgtggctt cgaatcttac tgggaaatcc ctgctggttc cgatacagca
     7321 gcacctggtg agtgggtgaa aggtccaggc tacaagcttt ttgcagccgt taaggaagaa
     7381 cttggtgagc taaacatcat cgcagaagac cttggcttca tgacagatga agtgatcgaa
     7441 ttgcgtgaac gtactggctt cccaggaatg aagattcttc aatttgcctt caacccagaa
     7501 gacgaaagca ttgatagccc acacttggca cctgctaact cagttatgta cacaggaaca
     7561 cacgataaca atacggttct tggttggtac cgtaatgaga ttgatgatgc gactcgtgag
     7621 tacatggctc gttacacgaa ccgtaaagaa tacgaaacag tggtacacgc tatgcttcgt
     7681 acagtatttt catcagttag ctttatggca attgcaacta tgcaagattt actagaattg
     7741 gatgaggcag ctcgtatgaa cttcccatct acccttggtg gaaactggtc ttggcgtatg
     7801 actgaagatc aattgacacc agctgtcgag gaaggtttgc ttgacttgac aacaatttat
     7861 cgccgaatta atgaaaattt ggtagattta aagaaataag acaataatca ggagacaact
     7921 aaacatgtta tcactacaag aatttgtaca aaatcgttac aataaaacca ttgcagaatg
     7981 tagcaatgaa gagctttacc ttgctcttct taactacagc aagcttgcaa gcagccaaaa
     8041 accagtcaac actggtaaga aaaaagttta ctacatctca gctgagttct tgattggtaa
     8101 actcttgtca aacaacttga ttaaccttgg tctttacgac gatgttaaaa aagaacttgc
     8161 agctgcaggt aaagacttga tcgaagttga agaagttgaa ttggaaccat ctcttggtaa
     8221 tggtggtttg ggacgtttgg ctgcctgctt tatcgactca attgctactc ttggtttgaa
     8281 tggtgacggt gttggtctta actaccactt tggtcttttc caacaagttc ttaaaaacaa
     8341 ccaacaagaa acaattccaa atgcatggtt gacagagcaa aactggttgg ttcgctcaag
     8401 ccgtagctac aaagtaccat ttgcagactt tactttgaca tcaactcttt acgatattga
     8461 tgttactggt tatgaaacag cgactaaaaa ccgcttgcgt ttgtttgact tggattcagt
     8521 tgattcttct attattaaag atggtatcaa ctttgacaag acagatatcg ctcgcaactt
     8581 gactctcttc ctttacccag atgatagtga ccgtcaaggt gaattgctcc gtatcttcca
     8641 acaatacttc atggtttcaa acggtgcgca attgatcatc gacgaagcaa tcgaaaaagg
     8701 aagcaacttg catgaccttg ctgactacgc agttgtccaa atcaacgata ctcacccatc
     8761 aatggtgatt cctgaattga ttcgtctttt gactgcacgt ggtatcgatc ttgacgaagc
     8821 aatttcaatt gttcgtagca tgactgccta cactaaccac acaatccttg ctgaagcgct
     8881 tgaaaaatgg cctcttgaat tcttgcaaga agtggttcct cacttggtac caatcatcga
     8941 agaattggac cgtcgtgtga aggcagagta caaagatcca gctgttcaaa tcatcgatga
     9001 gagcggacgt gttcacatgg ctcacatgga tatccactac ggatacagtg ttaacggggt
     9061 tgcagcactc catactgaaa tcttgaaaaa ttctgagttg aaagccttct acgaccttta
     9121 cccagaaaag ttcaacaaca aaacaaacgg tatcactttc cgtcgttggc ttatgcatgc
     9181 taacccaaga ttgtctcact acttggatga gattcttgga gatggttggc accatgaagc
     9241 agatgagctt gaaaaacttt tgtcttatga agacaaagca gctgtcaaag aaaaattgga
     9301 aagcatcaag gctcacaaca aacgtaaatt ggctcgtcac ttgaaagaac accaaggtgt
     9361 ggaaatcaat tcaaattcta tctttgatat ccaaatcaaa cgtcttcacg agtacaaacg
     9421 ccaacaaatg aacgctttgt acgtaatcca caaatacctt gacatcaaag ctggtaacat
     9481 ccctgctcgc ccaattacaa tcttctttgg tggtaaagca gctccagcct acacaatcgc
     9541 tcaagatatc attcacttga tcctttgcat gtcagaagtt attgctaacg atccagcagt
     9601 agctccacac ttgcaagtag ttatggttga aaactacaac gttactgcag caagcttcct
     9661 tatcccagca tgtgatatct cagaacaaat ctcacttgct tctaaagaag cttcaggtac
     9721 tggtaacatg aaattcatgt tgaacggagc tttgacactt ggtactatgg acggtgctaa
     9781 cgtggaaatc gctgagttgg ttggagaaga aaacatctac atcttcggtg aagattcaga
     9841 aactgttatc gacctttacg caaaagcagc ttacaaatca agcgaattct acgctcgtga
     9901 agctatcaaa ccattggttg acttcatcgt tagtgatgca gttcttgcag ctggaaacaa
     9961 agagcgcttg gaacgtcttt acaatgaatt gatcaacaaa gactggttca tgactcttct
    10021 tgacttggaa gactacatca aagtcaaaga gcaaatgctt gctgactacg aagaccgtga
    10081 cgcatggttg gataaagtca tcgttaacat ttctaaagca ggattcttct catctgaccg
    10141 tacaatcgct cagtataatg aagacatctg gcacttgaac taatactctt cgaaaatctc
    10201 ttcaaaccac gtcagcttta tctgcaacct caaagcggtg ctttgagcaa cctgcggcta
    10261 gcttcctagt ttgctctttg attttcattg agtataagat gcaaatttat actaatacat
    10321 tttgtaaaaa agcgagtttc gattgaaatt cgctttttta aatgctgtgg atttgggtca
    10381 atcttgtcta aaaataggga aatcctagat acagtgaagg ctttaaatgc tggtttttac
    10441 tgtcttcagc cttatatttt ttcgtagttg gttacctcat atctcttgta ttcgcttaca
    10501 taaagtatta taatataatt gtaggaaaga aggtgttttt atgatataca cacttaaatt
    10561 ggtgttgttt attacctttc ttgtaataag cttgttacct gataagattt ttggaaaaaa
    10621 taaaaaaatt tggaaaatag tttttgcaat attgacggca gtggcagcat ttatatttat
    10681 gtactaagtt attttaagaa tgtagggaaa taaaccctac attcttttta gttttttctg
    10741 ttttctaaat tctatttatc caagcgcttc aacatttctt gcttcttcgc ttcaagttct
    10801 gcacgctttt cttcgatttc ggcatgtttt ttctcgagtt cagaacaact tgcaccattg
    10861 ctaaattctt ttcgccatca ggagataggg tgagtcgaca tgtctattac tcacccaaag
    10921 cagtcctaca aagcaggaat tttctgttac ttttttggaa atagtaacgt ttatacagct
    10981 ttgacacttc gtatcaaagc gccaaacaca ctccgaggga tttacagaaa gcagaaaagg
    11041 aatgatctgg tataagatca ttccttttct ctctttttct ttaagtaatt atatacaatg
    11101 tacgacgaag tcgtcattgc aatgctgatc caccacctaa agggaacttt aaacaacatt
    11161 gataagataa agaatataaa caacgaaaat acgttatacc caattaattt tattgtatat
    11221 ctcatgatta aaagttaatc cttccgttgt taggaatggc atcattttta tcccataatt
    11281 gtgttaaata agtccccggt gataataaat tcatagcgga ttctaaagca acatcattta
    11341 caaaccaact acctagatat ctagaaattg ctgaacgaat agcacttttt gctgcatgtt
    11401 ttccttttac tttaattaga tttgcaaggc ctgcagtagt tcctcctaat gctaaagcta
    11461 ttgcagtatc taatagagca cccatttgat taactgtaat accttgccaa gctgctctaa
    11521 atggagagta tgtaggtggg attgtataat cgccttgtaa ttgtcggtta attacttctt
    11581 tgatccattg ttgtgagacg tctggatgaa aagattggat ttcgtttgca agtgtattga
    11641 tttgttcttc tgttagagaa gtgacaggtt gaagttccat atttgtttca atttgtgata
    11701 cttgttcaga agcgtataca gctgaaacac ttggaatcgc tgatacaatt aacacaattg
    11761 acgtcaaaaa aaccgaaata aatttcatta atttgttcat gagcttttct cctttttatt
    11821 tgtatttgct tacattttat tatatactgt tattatagtc aagaaaaata tgctattatg
    11881 ttaaaaaaat atttttcaaa atataaatgg acggatttat tttggattct ttttgtaatt
    11941 ggggcatgcc tcttgacggg taatagtagt cttttccccc ttacccatca agaattctct
    12001 tttcgtggta gcgtttgggg tctggtactg gccttatatc acttattgtt cattgataag
    12061 tttgtaattt cgaatcgaaa ataaaaatta gaactatgat tgactggcta cttttaatat
    12121 tgattttgga ggaagatttt gtctctatta tttattattt taaatttatc tattttgtac
    12181 aagatttatt ctttgagacg ggagaagtcg aaatacttga tttatacggc ctatatcata
    12241 tttggggtaa atgtagtata tggtattcaa tggttattaa aagaactgat ttcaactatt
    12301 tccacttaat atgtatgaaa actataacaa aacgcataag attaaggttt ttaagcacct
    12361 gttcttatac gtttttgtaa tattgaagat tttcggtaag ttttctactt ttgcgaagtc
    12421 tagtttagaa tgctttccca accaaaatct ctgcttcgat ggtaatctct gtcagttggc
    12481 tccagtcaat cttgctctgg tcaacgtgaa agaggagagg agtcatactg agtagggctt
    12541 ggagttgttc tgctgtgata gtcttagtca gagaggcagt ttgactagat aggatggtaa
    12601 agtgttcctg gaaatgctcc ttgatatctt ggttagaata ctccttgttt gtcagctggt
    12661 cctgtactct ttgacggatt tctttgaggt gattttcagt tgggataacc tttatcaaga
    12721 taccgtcttt ggataaaacg cgacgaaatt ctccatagtt ggcaggtgag aagatatcaa
    12781 gcagaatatc catgttagcg tcttttatag gaagtcgtgc caagtcgcca acgaaccaat
    12841 tgactgccca gttgggttca cttttagcag cgatttggac tgaatctttg gagatgtcaa
    12901 aggcatagaa agttttttca gagtgacttt cttgtagttt gcgagaatag aatccttcac
    12961 cacaaccgat atccaaaatt gttgtggtag tttttgagct tgcaagcaag tcagatacag
    13021 catctaagat agcttggtaa aagccggctt ctaggatttg ttgacggttt tgaaaatttt
    13081 ccttgtcata gttagcagat tgcttgattt gaggtgctag attgacatag ccaaatttcg
    13141 ccaagtcaaa agaatgacgg ttgcagcact tgaaattagt ctctaacaga gtcagatttt
    13201 cttgacagat aggacaggca aaggcagtcg cagaagcaaa acgctgaagt ttgggcttga
    13261 gatttgtatt catagtttaa gtgtatcaaa agaggcaaat gaaagcaact ttaggaataa
    13321 gtagatggga gattcagtag aaataaaact aattctccaa tttatagaat gaaattgctt
    13381 ttatatctaa attgctatat aataatgata aggaggaaat aagtatgtta aaagaagtat
    13441 taaccgttgc aaaagttgcg aaaaaatctt cattattttt gggtggtgtc gcatttggta
    13501 cccttggttt gaaaatctta gcaagtaagg aagctaaaaa aggttattct aaagctttgg
    13561 ctaaggctta caagttgaaa gacgagctag atgcatctgt ttctgttgtg aagcaacatg
    13621 gagacgatgt cttgcaagat gccaaatatt tgtacgagca agagaaaaaa gaagagcaat
    13681 tagatagcct tataggtgaa taatatgtct tttaaagtgc tacatagagg ataccaacat
    13741 atccgactat catcttcttt ttcacttacc ttggatattc aagactatct tcgttccttg
    13801 gcgagagatg aaaaggggat tgagtctatc cagttttaca tggatcaaca gcactttact
    13861 ctacgcataa aagaaggctt ttctgtatta gataatgcag aagccttttt aaaaagaatt
    13921 gataaaggga aagtttctga gttgatgact cttcccattc gtagagaaga gagtgcttat
    13981 tctattgttt caggtgcagc ggttaagcgt gtactttttc gtagttttgt gccgtatcct
    14041 attcgctata tatggacttg ttatcaggct ttgggttata ttagagaagc ctatcaaaca
    14101 ctagcgcgta aggaactaac gatggaagtc ttggactgtt cggctatttt attgtccttg
    14161 tttatgaacc aatccaagac agctagcaat atcatgttta tgcttgattt ggggaatcat
    14221 ttagatcagt ggtccttgaa aaaaactgca acagatttag aacagagtct tcttgcaaaa
    14281 gagagcgatg tattcctagt acagggcgat acggttgtta gtataaagag ttccgatgtt
    14341 caaataggag atgtcttgat cttatctcaa ggaaatgaaa ttctgtttga tgggcaagta
    14401 gtttcaggtt taggtatggt caacgaaagt tccttgacag gagagagttt tccagttgaa
    14461 aaaagagagt ctgatttggt ttgtgcaaat acagtattag aaactggaga gttacgcatt
    14521 cgtgtaacag ataatcagat gaacagccgt attttacagc tgattgagtt gatgaagaaa
    14581 tctgaagaaa acaagaaaac gaaacaacgc tatttcatca agatggcgga taaagtcgtc
    14641 aaatataatt tcttgggggc tgggctgact tacctattga caggttcttt ttctaaggct
    14701 atttctttcc tattggtcga tttctcctgc gctttgaaaa tctctactcc tgtagcttat
    14761 ttgacagtta tcaaggaggg gttgaaccgt gaaatggtga ttaaggatgg agatgttctg
    14821 gagaaatatc tggaagttga tactttcttg tttgataaga caggaacaat cacaactagt
    14881 tatcctatag ttgaaaaggt gttacctttt ggagactata gtgaggaaga tattctcaga
    14941 atcagtgcct gtcttgagga acacatttat catcctattg ctaatgccat cgtcaagcaa
    15001 gctgagatag agggaattga acatgaggaa atgcatggga aactccaata tatcgcaagc
    15061 aaggggatca aatctcatat agatggccaa ccagttctta ttgggaatta tgtcttgatg
    15121 caggatgagc agattcatat cagttcagaa caaaatgctt taattgaaga gtacaagagt
    15181 cactacaatc tcttattctt ggcttatcaa aatgaattga ttggaatgtt ctgcattcat
    15241 actcctttga gaaaagaagc aaaaacagcc ttggataaac ttaaggcaca ggggaaaaaa
    15301 ttgattctgg caacaggaga caccttgatt aggacagagg aattagtcaa agatttgccc
    15361 tttgatcagg tctatacaga cttgaaacct gatgggaaat ttgagttagt agagaaactg
    15421 cagaaagcag gtcacactat tttgatggtt ggagatggct tgaatgactc agcggcttta
    15481 actctatcag atatcggtgt ggtgatgaat gagagtgcag atatttctaa gcagatgagt
    15541 gatatcttat tgttagataa tcgtttggat ttcttccaag agttggattc gttatcatca
    15601 tctttgcaaa cactcatcaa gaaaaatatt caggataccg ttgtcgtaaa tagtagtttg
    15661 attggctttg gcttatttaa ttggttcagt ccttcaaatc tctctatctt acataatcta
    15721 acaaccttgc gcattgtcct gcgtagcctg tctattaaaa atagatagat gagagttatt
    15781 aacagcaaaa aggagttacc tttctcgagg ttaactcctt tgtttataga taaatgttga
    15841 atagtttagt aagtcaatac aaagtatttt ttcttcccac gacggataac agtcagttca
    15901 ttttctaact tatcagcgtc actcaagaca tagtcaaggt cttggatgcg gtcgccgttt
    15961 acgtagatgg ctccgttttg gacgtcttca cgggcttggc gttttgagtt aactatacca
    16021 gatgagacga gcagttccac gatattgttg ttttcgtctg cctgtacttg gtagtttggc
    16081 acaccacgaa gtccttgttt gagctctttg acagaaaggt ttttgatgtt tcctgcaaag
    16141 agttgctcag tgatgttaag tgcttctttg taggcttctt ctccgtgaac aagtgtaaca
    16201 acttcacgag ccaagacttt ttgagccaag cgttcgtgtg gcgctgcttc aaattgttta
    16261 cgaatatctt caatctcatc aagtgacaag aaagtaaaga ttttcaagaa gcgaacagcg
    16321 tcagcgtcca tcacgttcat ccagaattgg tacatttcgt atggagaagt cttttcggga
    16381 ttgagccaga cggcatttcc ttctgattta ccaaatttct taccagttgc atctgtgatt
    16441 agtggaacag tgataacgtg accagtcttg tccgccttac gacgaagcaa ttcggtacca
    16501 gctgtcatat ttccccactg gtcagaacca ccgatttgaa gagtgacatt atggtcttgg
    16561 ttaaggacga agaagtcgta cccttgcatg atttggtaag cgaactcagt gtaagaaatt
    16621 cctgtttcga tccgtttttt aacagattcc ttactcatca tgtagttgac cgtgaagtat
    16681 tttccaatat cacggaggaa gtcaatgaag ctgatgctgc caaaccagtc gtagttgttg
    16741 accatgacag ccttgttttc gccattttca aagtcaagaa aacgagaaag ttgtccttgg
    16801 atagacttga cccagccatc tactgtgtct tttgtttgga gactacgttc agcatctttg
    16861 aaggacggat ctccgatgag acctgtagca ccgccaacga gcgcataagg tttgtgacct
    16921 gctagttgca agcgacgact tgtcaagatt gcgacaaggt ggcctaggtg aaggctgtca
    16981 gcagttggat cgtagccagt atagtaagaa acttgacctt cttctagggc tttacgcaaa
    17041 gcttcttcat cagtcgtttg aaatatcaaa ccacgctctt ttagctcatc aaaaatgtgc
    17101 atgtgtcttt tctccttttt aaaattattg tttctaccta ttgtatcaca aacttgggca
    17161 atagcctagc agaataggga agaaagtggc tattttattg aaaatttccc ttttgtggta
    17221 taatagataa agtgaggaaa tccatgcaaa atcaattaaa tgaattaaaa cgaaaaatgc
    17281 tggaattttt ccagcaaaaa caaaaaaata aaaaatcagc tagacctggc aagaaaggtt
    17341 caagtaccaa aaaatctaaa accttagata agtcagccat tttcccagct attttactga
    17401 gtataaaagc cttatttaac ttactctttg tactctgttt tctaggagga atgttgggag
    17461 ttgggattgc tttggggtac ggagtggcct tatttgacaa ggttcgggtg cctcagacag
    17521 aagaattggt gaatcaggtc aaggacatct cttctatttc agagattacc tattcggacg
    17581 ggacggtgat tgcttccata gagagtgatt tgttgcgcac ttctatctca tctgagcaaa
    17641 tttcggaaaa tctgaagaag gctatcattg cgacagaaga tgaacacttt aaagaacata
    17701 agggtgtagt acccaaggcg gtgattcgtg cgaccttggg gaaatttgta ggtttgggtt
    17761 cctctagtgg gggttcaacc ttgacccagc aactaattaa acagcaggtg gttggggatg
    17821 cgccgacctt ggctcgtaag gcggcagaga ttgtggatgc tcttgccttg gaacgcgcca
    17881 tgaataaaga tgagatttta acgacctatc tcaatgtggc tccctttggc cgaaataata
    17941 agggacagaa tattgcaggg gctcggcaag cagctgaggg aattttcggt gtagatgcca
    18001 gtcagttgac tgttcctcaa gcagcatttt tagcaggact tccacagagt cccattactt
    18061 actctcctta tgaaaatact ggggagttga agagtgatga agacctagaa attggcttaa
    18121 gacgggctaa ggcagttctt tacagtatgt atcgtacagg tgcattaagc aaagacgagt
    18181 attctcagta caaggattat gaccttaaac aggacttttt accatcgggc acggttacag
    18241 gaatttcacg agactattta tactttacaa ctttggcaga agctcaagaa cgtatgtatg
    18301 actatctagc tcagagagac aatgtctccg ctaaggagtt gaaaaatgag gcaactcaga
    18361 agttttatcg agatttggca gccaaggaaa ttgaaaatgg tggttataag attactacta
    18421 ccatagatca gaaaattcat tctgccatgc aaagtgcggt tgctgattat ggctatcttt
    18481 tagacgatgg aacaggtcgt gtagaagtag ggaatgtctt gatggacaac caaacaggtg
    18541 ctattctagg ctttgtaggt ggtcgtaatt atcaagaaaa tcaaaataat catgcctttg
    18601 ataccaaacg ttcgccagct tctactacca agcccttgct ggcctacggt attgctattg
    18661 accagggctt gatgggaagt gaaacgattc tatctaacta tccaacaaac tttgctaatg
    18721 gcaatccgat tatgtatgct aatagcaagg gaacaggaat gatgaccttg ggagaagctc
    18781 tgaactattc atggaatatc cctgcttact ggacctatcg tatgctccgt gaaaagggtg
    18841 ttgatgtcaa gggttatatg gaaaagatgg gttacgagat tcctgagtac ggtattgaga
    18901 gcttgccaat gggtggtggt attgaagtca cagttgccca gcataccaat ggctatcaga
    18961 ccttagctaa taatggagtt tatcatcaga agcatgtgat ttcaaagatt gaagcagcag
    19021 atggtagagt ggtgtatgag tatcaggata aaccggttca agtctattca aaagctactg
    19081 cgacgattat gcagggattg ctacgagaag ttctatcttc tcgtgtgaca acaaccttca
    19141 agtctaacct gacttcttta aatcctactc tggctactgc agattggatt gggaagactg
    19201 gtacaaccaa ccaagacgaa aatatgtggc tcatgctttc gacacctaga ttaaccctag
    19261 gtggctggat tgggcatgat gataatcatt cattgtcacg tagagcaggt tattctaata
    19321 actctaatta catggctcat ctggtaaatg cgattcagca agcttcccca agcatttggg
    19381 ggaacgagcg ctttgcttta gatcctagtg tagtgaaatc ggaagtcttg aaatcaacag
    19441 gtcaaaaacc agggaaggtt tctgttgaag gaaaagaagt agaggtcaca ggttcgactg
    19501 ttaccagcta ttgggctaat aagtcaggag cgccagcgac aagttatcgc tttgctattg
    19561 gcggaagtga tgcggattat cagaatgctt ggtctagtat tgtggggagt ctaccaactc
    19621 catccagctc cagcagttca agtagtagtt ctagcgatag cagtaactca agtactacac
    19681 gaccttcttc ttcaagggcg agacgataat tctctaaatg aagtggccaa tcaatggatt
    19741 gccacttttt tcttttcgtg ttacaataag ggtatgaatc agtatcagaa aaagattgtt
    19801 aacggaaaaa tttattcgct cctatccggc ttaatatggg gaatctgtgg aattttagga
    19861 gagtacttct ttactcatta tcaggtgtct tcgggctgga ttacctctat gcgtttgaca
    19921 ctggcaggga gtcttgtact catttggtct gcaatacaat taaaatcgca agtgctagat
    19981 atttggcgag acaagaaaaa ttacctgccc tttttagcct atgctatttt ggggattttt
    20041 tcagttcagt attttttcta tctctgtgta gaatactcaa atgctacgac agcaactatt
    20101 ttacagttta ttagccctgt ctttatcctc ttttacaatc gcttggttta tcaaaaacga
    20161 gcgtcaaaaa gcgctgtttt ctatgttttg gttgccatgc tgggtgtttg cttgatggcg
    20221 acaaagggag acctctctca gttatccatg acgccgctag ctcttataac aggtttgctg
    20281 agtgccatgg gtgttatgtt taatgttatc ttgccccaac cttttgctaa gcgttatggt
    20341 tttgttccta cggttgggtg ggggatgatt ttggcaggtt tgtttagcaa tgtcctctcg
    20401 ccggtttatc agctttcctt tactcttgat atttggagta tcttgatttg cctcattatc
    20461 gctttctttg gaacggcttt tgcttttttc atttccatga aggctgtgtc cttggtttct
    20521 cctttggtgg ttgccgttat cagtgccagt gaacctctct cttctgctct cttgagtgtt
    20581 ttgttcttag gattagtagt ggattggtcc ctccttctag ctatagcctt gattatttta
    20641 cccatgatat ttttgtctat agaagaagcg aaagaaagta gataaaaagt cttttcttaa
    20701 ttctaaaagt gtgctatact ggaatagtca ataaaacacg gggagatagt tgtgaagagt
    20761 gtttttaggt tgtatcggta ggggcttgct tttaccgaca gcacgtttta tctcacatcc
    20821 ctaaaaaatc atacctaaaa acagacaaaa aggtttcaaa tttgaggcct tttttggtag
    20881 aataggtatc attataacga accaggaggc acctatgact gctacaaaaa tgaacgctca
    20941 agaaattatc caatttatcg ccaatgctga aaagaaaacc agtgtcaaag taacctttga
    21001 ggggcaactc gcaactgctg tgcctagctc tgttgtcaaa ctaggaaatg tcctattcgg
    21061 agactggaag gacgtggctc cgcttcttga aggtttggta gaaaatcaag attatgttgt
    21121 cgagcaagat gctcgtaatt ctgcagttcc tttgctagat aagcgtgcta tcaacgctcg
    21181 tatcgagcca ggtgctatta tccgtgacca ggtggaaatt ggtgacaatg ctgttatcat
    21241 gatgggagct gttatcaata tcggtgctga aatcggtgct ggaaccatga ttgacatggg
    21301 tgccatcctt ggtggccgtg ccattgttgg aaaaaatagc cacgttggtg caggtgcagt
    21361 tttggcaggt gtgattgagc cagctagtgc tgaaccagtc cgtgtcggag acaatgttct
    21421 catcggggct aatgcagtgg ttatcgaagg agtccaaatc ggtagtggtt cagttgtcgc
    21481 agcaggagct attgttaccc aagatgtccc agaaaacgtg gtagtagcag gtgttccagc
    21541 tcgtattatc aaagaaattg atgcccaaac tcaacaaaaa acagcgctag aggatgcgct
    21601 tcgtaccttg taattgtaaa agtaaaaaag aggcggaacc ctttttccag cctctttctg
    21661 ctatatcgga ggacagatag atgttagatt tgattcagac tagacgagat ttacaccaga
    21721 ttccagagat tggcttggag gagttcaaga ctcaggctta tttgctggat gtgattgaga
    21781 aattgactac gggcaaggat tttgttcaaa ttcgtacttg gcggacaggg attttggtct
    21841 acctgcaggg aagtcagccg gagcgaacca ttggttggcg gacagatatt gatggcctgc
    21901 ctatcgtcga acaaacaggt ctaccttttg cttctcaaca ccaaggtcgc atgcatgcct
    21961 gtggtcatga ttttcatatg accattgcct taggctgtct tgagcgcgcc cttgaggagc
    22021 aacccaagaa taatctgctc tttctatttc agcctgctga agaaaatgaa gctggtggta
    22081 tgctcatgta tgaggacgat gcttttggag attggttgcc agatcaattc tatggtctcc
    22141 atgttcgtcc agatttgaaa gtcggccaga ttgcgactaa tactcataca ctctttgcag
    22201 gaacttgcga ggtgaagatc cgtttcaaag gcaaaggtgg ccacgcagct tttcctcatg
    22261 aagccaatga cgccttggtg gcggctagtt actttgtgac ccaagtgcag tcagttgtta
    22321 gccgcaatgt caacccaatc gagggagcag tggtgacctt cggcgttttt caagctggaa
    22381 ccaccaacaa tgtcatcaca gacacagctt ttttacatgg aactattcgt gccttgacac
    22441 aggacatgag tctcttggtg caaaagaggg taaaaacagt tgcagaaggg gttgcagctg
    22501 cctttgatat ggaagtcgaa gtggaactca aacaaggtgg atacctacct gttgagaaca
    22561 atccagcctt ggcgcgtgaa ctgatggact tctttgacga gaaagacgga atcgagttga
    22621 ttgatatcga acctgctatg acaggcgagg actttggtta tctcctttca aaggttgatg
    22681 gcgtgatgtt ttggctaggt atcgatagtc cctacgctct tcatcaccct caaatgagtc
    22741 ctaaggaaga agtcttagct attggggtgg ctgcggtgtc tagtttcctg aaaaagaagg
    22801 cagcagaata gagggcttgt ctatgaaatc ggaattacgc aagcaagtct tgcatgaaat
    22861 gaaggctata tctcaagagc aaaaacaggc tatagaccaa gctttaaccg agcggctttt
    22921 acaccatccc ttctaccaag aagccaaggt catcgcaacc tacctctctt tttctcatga
    22981 gtttcaaacg cgggaactga ttgagcaggc gctgaaggac ggcaagaagg ttttaatacc
    23041 caaaacttat cccaaggggc gcatggagtt tgtggcctat gatccgcaac agttggtaaa
    23101 aacttccttt gccttactgg agccacaggg agatgtggaa gtggtggatg catctcagat
    23161 tgatttgatt catgttcctg gtctggcttt tacgacgaaa ggatatcgga taggatacgg
    23221 tggaggctat tatgaccgct atctggaaca tttttctggt cgtactttga gtacgatata
    23281 tccttgtcaa attcaggact ttatacctga aaaccatgat attcctgttc aggaggtatt
    23341 aattgatgaa ggaaatcttt gatagacgtt accctgtgac gagtttcttc ctcttagtga
    23401 cggccttggt atttttacta atgttggtca ctgcaggcgg aaactttgac agggcagata
    23461 cattatttcg atttggagcc atgtatgggc cagctattcg cctctttccc gagcaggttt
    23521 ggcgtctctt gtctgccatt tttgttcata ttgggtggga acatttcatt gttaatatgc
    23581 tttcacttta ttatcttgga aggcaggtag aggagatttt cggttctaag cagtttttct
    23641 ttctctatct tttatcagga atgatgggca atctctttgt ttttgtattt agtcctaaat
    23701 ccttagcagc aggcgcctct acctctcttt atgggctatt tgccgcgatt attgttcttc
    23761 gctatgcaac tcgcaatcct tatatccaac agctagggca atcctatctg acactttttg
    23821 tggttaacat tattggaagt gttctgattc caggaatcag cctagcaggc catatcggtg
    23881 gtgcagttgg tggcgcattt ctagcagtta tctttccagt tagaggagaa aaacggatgt
    23941 ataacaccag ccagagatta ggagcggtag tcttgttcgt aggactcgcc attttgcttt
    24001 tctacaaggg aatgggaatg tgacgaagtt gaaagaaaat ttgtgtaatg aaaaaagata
    24061 aggcattttt tgaaccttat ctttttattt tattccttct cagtcaattc ttttccaagt
    24121 tggatgaggt aattcttcaa atcatctttg acttgtggat gtttgagggc gtagtcaata
    24181 gatgttttca taaagccaaa cttatcccca acgtcgtaac gagctcctgt aaattcacgg
    24241 gcaaaaacac gctgtgtttt attaagagta tcgattgcat cagtaagttg gatttcattt
    24301 ccagcaccag gagcttggtt ttcgaggatt tggaaaattt caggcgtgag gaggtagcgt
    24361 ccgataatag caaggtcgct aggagcgtcc tctggagctg gtttttcaac aaaggtttca
    24421 acactgtaaa gaccatcttt tccttcgcct tgcggagcaa taaccccgta agcagatact
    24481 tcgtcatgag ggactggcat gacagcgata gtagacgcgt gggtacgctt gtagtcatcc
    24541 atgagttgtt tggtaagtgg aacagccttt tcgtctgtga tatccatcaa gtcatcacca
    24601 agcataacga caaaaggttc atttccgacg aaagccttgg cttgcaaaac agcatctccg
    24661 agaccgcgtg gatgagtttg gcggataaaa tgcagacgca tgccagttgt ttcatcaact
    24721 agcttcaaaa gatctgtttt ccctttttct ttgaggttat attccaattc gaagtttgaa
    24781 tcgaagtggt cctcaataga acgttttgat ttaccagtga caactagaat atcttcaata
    24841 cctgatttga gagcctcttc cacgataaac tggatagttg gtttgtctac gattggcaac
    24901 atttctttgg caagggcctt ggttgctggt aaaaatcgag ttcctagtcc agcagcaggg
    24961 ataactgcct ttctaacttt tgatgtcata aaaatccttt ctatagaggg ttaagaccac
    25021 tcattttctg ctttaaattc attgttcatg atgtcataaa tggcatcttt gatattggtt
    25081 ccgtgataaa taacttggta aatagcctgt gtaatgggca tatagactcc aagttcttgg
    25141 gctagttcat aggctgctcg agtcgttgaa attccttcga ttaccatgcc catattagct
    25201 tctatatcag ctagggattc tcctcgtccg agagcatctc cagctctcca gttacgagag
    25261 tggatggaag ttcccgttac gatcaaatct cccacaccag ataagccgct ataggtcaat
    25321 ggactggccc cgagtgctac ccctaggcgg gtgatttctg ctaaacctcg agcgatgatg
    25381 gctgccttag cattatcacc aaatccaaga ccatgtaaag ctccagcacc gacagcaata
    25441 atatttttaa gagcaccagc agtttcaacc ccgataacat ccgtattggt ataaagtcgg
    25501 aagtagtgat tactaaatag ctcctgaacg tattgagctg tttgtaaatc tttagaagca
    25561 gcagttatta aagttaggtc acgcacaatg gtctcttctg catgactagg ccctgaaaca
    25621 acgacgatat cactacggag atgttcagga atttcttctt caagaatggt tgataatcgt
    25681 ttatggctat caggttctaa tccctttgat gcgtgcatga tgataacctt atggtccaag
    25741 gtttgtgcaa cttgctgggc aacaagtcgt gtcacttttg ttgggacaac aaacaaaatc
    25801 gcatccacat ttttcaatgt ttctgctaag tcggtgtagg caatgatatt ttcgtctaga
    25861 acgacatctt taaagtagtg cttattagta tggtgtgtat taatttcatt gatttgctcg
    25921 ggaagatttc cccaaatacg tacctcgtgt ccattgtcat ttaagacttg tgaaagggcg
    25981 gttccccaag aaccaggccc caagacggcg acggtttgtt tttccatgat ttcctccttg
    26041 atagatttct ttctcttatt ctatcatatt ctaggggatt ttgcacttgc attactgggg
    26101 tatggtggaa aaatacacaa agaccgagat gaagggataa actcttagtc tttagttaaa
    26161 gtgagaagaa atctgataga tagtttgatt cagtatatgc ttataataga ggtaaacaac
    26221 tcaggaagtt cttgtaggtt gcgagagttt ggcgaaaaaa ttaaaagatt acgtttggct
    26281 aaaaaaatca gtcgttcaga attttgtggt gatgagtctg aattaagtat ccgtcaatta
    26341 attagaattg aaaatggaga atccagacca acactaacaa agttaaaata tattgctgaa
    26401 cgtttggagg ttgaagatta caagttgatg ccaagttata tagagttgga taaggaatac
    26461 ctagaattga agtatttctt gatgaggact cctacatacg aagatgaaac tatcgcccaa
    26521 aagaaagaga gtgtttttga taagattttt gaagagtatt atgataggct acctgaggaa
    26581 gaaagattta tcatcccaaa ttattcatat ctagcactag cgaactacac agttcaaaaa
    26641 ttaccagaaa agctagttga aatactgtcc ttctggtgat tttgcttaat ttctgatgtt
    26701 cgagacatga ttgaggagat ttgtcttgat tttttcctaa aaaagggcag actcctccct
    26761 tggtttgatg ctcgattttt aaaataaggt gacttattct gttaggcagt tttcccctgc
    26821 cctctagctc tatatggaac agggagtatc agatttatct ggcagattgt atcaaataag
    26881 gcttgaaact gttttgaata ggcgtataga cttgagaatt tgagaatatg tcgtctagca
    26941 gtagagacat attttccggc aatatgaagg aagagacgtc gaaaacgctt gatagtcaag
    27001 gcctttactt catcaccagc tagctgcttt aaaaagaggt agagattata agccagacag
    27061 cccatcatca tacgtacttc attcttaatc atggtcgaac tatctgtctt atccccaaag
    27121 aatcctgctt tcctttcttt gataaagttt tcagcattcc ctcgttcacg atagaattga
    27181 aacagctcct cactatctac actgcttaaa ttggtcaaat gaactttttt acaaccataa
    27241 aaaagagcac ttaatcgtac tcttctcttt taaggattag ttgaattaat tcaaatccac
    27301 tagttctcct gtttctaggt agactaggcg ttcacaaatg ttagcaatgt aatcagcgaa
    27361 gcgttccaga tgccctatga tataaaggta ttgagttcca ttgggaattg aggtttcttg
    27421 gtctttcata agtccaatga tttcctttga taaggcataa taatattggt caatctgttc
    27481 atctttttga gcaatactaa tagcttttga ggcttggtgc aaaggaaagg caaccaataa
    27541 atcggctagc atgctgaggg ataatttacc catttggtgt aactgttctt cgtcaggggc
    27601 tagttgattt tcttttagtt gcaaaacagc tttggcaatg cctgccatat ggtctcccat
    27661 acgttcaagg tctgaacaag aagacatgat gctaatcaca aatcgaaggt cagacacttg
    27721 tggctgctgc aaggccaaca aacgggcaca ggtcaattcg atagcgcttt gaccttggtt
    27781 gatagcatga tccttattga taattagctc tgccatctcc ttgtctttgg aggctaaggc
    27841 cagtaaggct tttgaagctg tttcaaggac aagttgccct agtcctaaaa aggattgttc
    27901 taattcatgc aattctaagt caaattgatt tctcataggt ttttccttcc ttatccgaac
    27961 cgtcctgaaa tatagtcttc tgtgcgctga tcttttggat tggtaaacac gtcaacggta
    28021 tctccaaatt cgcaaatttc tcctgttaag aaaaaagcag ttttatctga aatacgtgaa
    28081 gcttgttgca tgttatgggt aacaatgata atcgtataat ccttttttag ttgctgaatg
    28141 aggtcttcaa tttttaaagt ggagataggg tctaaggctg aagtcggctc atccattaac
    28201 agaatatcag gttctactgc taaagctcgc gcaatgcaaa ggcgttgctg ctgaccgcca
    28261 gataaggaca tagcactctt tttaagatca tctttgactt cttcccaaat ggctgcccct
    28321 tttaaagatt tctccactaa ggcatctaat tgttttttgt ctcgaatacc atgtgtcctt
    28381 gggccataag ccacgttatc atagatagac atggcaaagg gattaggctg ttgaaaaacc
    28441 atccctacac gcttacgtag ctgattaagg ttgaatttgc tactataaat atcttgctca
    28501 tctaagagga cttggccttc aatatggcaa gaaggaacca aatcgttcat ccggttaagg
    28561 gtttttagaa aagttgattt gccacaacca gatgggccta tcaaggcagt aatctgtctt
    28621 tctggtaatt gaatcgaaat attttttaag gcttgaaaat ccccgtaaaa taagtctagg
    28681 tgtctgactg aaaatgttcc catactagga agctcctttc acaagttttc gagataataa
    28741 gcttgataga gtatttatca ttaaaacagt aataatcaaa atcacaccgg tagcataggc
    28801 ttcattgaca tgtagcccct cacttgacag catatacata tgcagggcta gagaacgacc
    28861 tgaagacatg agactacttg gcgtattggt agaggtacct aatgtataca tgagggcagc
    28921 tgtctcacca acgatacggc caatagctag tatcactcca gctaaaatac ctggcatggc
    28981 aactggtaga acaattctaa aaacagtccg taacttacct gccccaagtc cataacttgc
    29041 ttgacgcatg atatcactaa cagataaaag ggcttcttct gttgagcgaa taatgactgg
    29101 caacaccatg atgactgagg ttaagattcc tgataacaga gagtattgaa aacctaagaa
    29161 gactacaaag aagagcatgc caaacagacc aaaaacaatg gaaggaatcc cagacaaggt
    29221 atctgaggcc aatcgcatga ttttaacaca aagggaatct ttttttgtat attccacaag
    29281 ataaaaacca gcaaaaatcc ctatgggcaa ggctaaaaga agagcaccaa agaccagaat
    29341 aacggtggaa ataatcgctg gcataaggga aatgttctca gaagtataag tccaagaaaa
    29401 gagggataga cttagatgag gtaagccttt gatgaggata aaaccaatga ttaaaaagag
    29461 agagccaaag gttaaagctg aaaaacaata aacgagaagt tttagcaggt atttactcat
    29521 aagatgattt tcctttcaag taggcaaagt aggcattaat caagagaata aggaaaaaga
    29581 gaactgctga ggttgcaata agggcttccc tatgctgacc tgatgcgtaa gccatttcca
    29641 gaacaatatt ggttgttaag gttctggttc ctgaaaagag tccacttgga ataatcggct
    29701 ggttgcctgc caccaaaata actgccatgg tttcacctac tgcgcgaccg attcctaaaa
    29761 taactgctga taaaatacca gatctcgcag ctggcaagat gacactaaaa atactccgtt
    29821 catgactagc tcctagagcc aagctaccag aataatacgt tttgggaact gttcggatag
    29881 cagattctga caaactgata atggttggca aaatcattat tcctaatagt aacgaagcgg
    29941 ttaggacact catgccattt cctaaaaagc ttctaatcca aggcaccaat aattgtaggc
    30001 cgaaaaaacc ataaacaata gatggaatgg ccgccatcaa gttgatagct gattttaaga
    30061 agccatagac gggctttgga caataataaa ccataaacac cgatgtcaag atgcctgttg
    30121 gcaccccaat cacaatcgct cctaaggtaa ttaataagga accaacgatc attggtaaaa
    30181 taccatagct tgccggaatg ttcgttggcg accaatcact gcctaataaa aaacgggcaa
    30241 agccgtagtt agccatgaaa ggtaagccat tactaaaaat aaagaaacag attagcaaaa
    30301 tagctacaac agctactgtt gcactcatga aaaaaattgc cctaaaaact gcttctttga
    30361 aggcttgttt tatcacatct tgtcctttct agtgaagaaa gtaagggaga tacgacacct
    30421 ccctacttgc cttctttatc ttattgtacg atgaaacgtc tgcatctctt tagagattta
    30481 tggagcaaac attttattta atcttgtccc aggtggttaa tttgccacta aaaacgtctg
    30541 caagttcagc catactgact tggcttgcct tattgtcatt attgaccaca acagcaatac
    30601 cgtctaaagc aatagcatca tgggtgagac tcttaccttc ttcaggagtt aattccctag
    30661 aaaccatacc aatatcagcg gttttctcct taacagcggt aatacctgct gaagacccat
    30721 tagaggtaat atcaatcgta acttctggat tttctttttt ataagcttct gctaattttt
    30781 ccattaaaga agatactgaa gtggaaccta caacagacaa cttgcctgat aagtgttggc
    30841 ttgtatattc tgtggtttca gttttagctt caataaattt attatctgtg accacttgtt
    30901 gaccttgttt ggagtggata aagctgataa aatcttgacc tagcttggaa agattagaag
    30961 accaaacaat gttgaaggga cgttgaagag ggtattcacc atctaaaact gtgtctcgac
    31021 tagccttgac accatcaatc tctaaagcct tgacagattt cgttaaagat cccaaggaga
    31081 tgtagccgat agcattagca ttcccttgaa ctgctgagag aacaccttct gtactatttt
    31141 gaatcacagc tgttttggca gtgttgtcaa tttttttatc accgtctttt ttgagaatcc
    31201 ctgtgatttc tgtgaaggca ccccgtgttc cagagccatt ttctcgtgaa atcacctcaa
    31261 tcgttcctga agctgactgt ttggaagcag ctgactgatt gccacaggca acaagcccaa
    31321 atcctgataa gccaatggct gcaagagtaa gcattttttt gaatttcata ataatcacct
    31381 ttatctctat gtatttttct tgtgtaggct tactacattt atagtctaac aagtctttgt
    31441 aaaggtttat ccctgattca tgtaaagatt gtgtaaagaa tcaaaaaaag ccacttttga
    31501 aaaatggctg ctcctaaaaa tagctttaaa aattattagt cctgtgcgaa agattggtta
    31561 ggaagaaaaa tcgtgaagca actgcctctg ccaagctgac ttgtcaccgt gacttggcca
    31621 cctaataatt gactgagttc tttgacaatg gcaaggccaa gaccagtgcc accagtttgt
    31681 ctgcttcgac ctttattaac tcggtaaaaa cgttcaaaaa tacgatcctg ctctaattga
    31741 ctaataccaa tccctgtatc tgatacagaa atcttaatgc cttcgttcac cttttgggtc
    31801 ttgacctcaa tttttccccc ttgttcagtg taacggatgg cattggataa aagattgagt
    31861 aagatttggg aaagtaattg actatctgat acgagggtga catcatctgg cacctgcacc
    31921 tttagctgta aatccttctt cttgagctga ggttgcaagc tttgagtcaa atcctgtaca
    31981 aattctgcca aagaaagggt cgtccattgt ataggcattt gttgagcctt agataaggta
    32041 agaagatgct caacaatatg ctcaagacgc aagctttctt tgtaaataat gtctagaaag
    32101 tcatccttga gcgcttcttc ttcagcagac atccccttaa tggtttcagc aaagccctta
    32161 atcgaagtaa ctggtgtcct caattcatgg gaggcatttg agacaaaggc taaatttaac
    32221 ttttcataag ttctaatcgt tgttaaatca tatagcaaga cgagcacagc ttccacagat
    32281 tgggtggggc taaaaacggg aactgctgtc acttctaaaa tcaagtcacc ctcatgaaac
    32341 ccacttactt cttgttttaa ccttgttttt tgatcaaagg cttggtgaac taaatttcga
    32401 atatccatcc gtttgaggtc atcaagtgaa cttatgtcgc cgtccacatc gggaaaataa
    32461 tgaggcagag agcgactgga taataacatc tgaccttgag cggaaactaa aaacgtcccc
    32521 atggttaggt gcgacagaag aacctccatt gtttcggcta ggtccttgta ttgctgatcc
    32581 tgttgggaga ctttggtttt taggccagac acatactgag ccaaagactt taagtcttct
    32641 tgcccttttt ctaaaaagta ttcactactg gtcaagagag gttggtgcaa ggtctcaaaa
    32701 gcaacttccc atttccaaag gcaaaagagc cagtagccac cttgtcccaa agaaagggct
    32761 agaagaaaga gaccgatgcc tttactgatc caagttaatg ccatccctgc aatcagaatg
    32821 aggctaacac ttagattgac tagccaaaat tgaaggtagc gtttcatcta taactccttg
    32881 aacttataac cataaccccg aatggttcga ataaattgag gggctttagg attgtcttca
    32941 attttttccc tcaacttacc aatatgaacg tccaccaaac gtgtttcctg cccaaagtca
    33001 tacccccaga tacgttccaa aagacgctct ctagtcagtg tcatgttggg atgtttcata
    33061 agatagagca agagttcaaa ttcttttggg gtcaaactca gtaacttatt cgccttgtag
    33121 acttcatgac gctcagggta tactttcaag gtcccaaata gccaagaatc gtcagcgata
    33181 ttatctgaat catctccttc ttgttctcct ttagttcgcc tgaggacagc cttgacacgc
    33241 gccagcaatt ctctagggct aaaaggcttg gtcaggtagt catcagcccc taattccaag
    33301 gccaaaacct tatcaaattc atcacttttc gcagaaacca tcataattgg agttttgacg
    33361 cctttggctc tcagccgctt acaaacttcc atgccatcta attgtggtaa catgatatca
    33421 agcaagataa aatcaaaggg ttctgtttct gccaaagcta aggccttccg tccatttgtt
    33481 accaattgag tagaaaagcc ttccttactt aaatggtagt caagcaattt cagaatgtgt
    33541 tcttcatcat ccactaataa gacttgtttt gtcatctatt atctcctatt ggtaacatta
    33601 taacacaatt atcagaaatc ctaacattgc taaatcagat taaatttgcc tatcaagact
    33661 agtatctggt caaacgctca atcatctcct tgtgctctgg ataggtcgcc agtagatcta
    33721 ccctttcaaa taattcaaaa tcctcaaatt caaaaccagg agcaacaaga caagaaacca
    33781 gagcatcatc cttatcaact gttgatcccc aaatagtgcc cttaggaaca cagtagtgaa
    33841 gttgttgccc tttggatatg tccaggccta aagtgactgc ttcgtagtga ccatctgctg
    33901 taatcatgtg aacagtaagt ggggatcctg catgaaaata ccagatttca tctgctgtca
    33961 atcggtgaaa atgtgaagga ttcgtttctt ctaataagaa ataaatactg gtataaagcg
    34021 cccttccctt accagcaagg tttatagtgt ctgaagcttt ttttgtttgt ctaaaatagc
    34081 caccttcaac atggggagct aactctagag ttcttatcaa gtcttcttta tccgtcggag
    34141 ccaatgggtt gaagtaactc ttgttcaaag tggttttacg atttcaagaa ctcctctcag
    34201 ttctgaggac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc ctaaaaaaag
    34261 aattagcgaa tgattctggt aaaaagaaat ttcacgctat gaaggctcaa gcgattgtca
    34321 caagtcaagg gagaattgtt tctattgcca tgatatgaag ttgttcaaaa tgagttgcag
    34381 aaatatcgga aaagctggta aaatcttggc tgacagtgat tatcaagggc tcatgaagat
    34441 atatcctcaa gcacaaactc cacgtaaatc cagcaaactc aagccgctaa cagctgaaga
    34501 taaagcctgt aaccatgcgc tatccaagga gagaagcaag gttgagaaca tctttgccaa
    34561 agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac gcttcggatt
    34621 acgaatgaat ttgattgctg gtattatcaa tcatgaacta ggattctagt tttgcaggaa
    34681 gtctattatt tggttaggtg aattagtgaa gtgtttaggc aagtgtctct ggttacgacg
    34741 tcatggcctc taaatcgatt atatttaggg gtcatgacta gtgaagcagt tagctagttc
    34801 gcatataagc ggctagcgtc taacaattag gaactttagt tccaataact ttaagattac
    34861 gacgttttag gacataaatc gatcatattt atgtcctaaa actagtgaag cgcctagcca
    34921 aagtccgaat aggatttggc gttagttact tagattgctt tgcaatcaag taactttggc
    34981 gatttacatc ttctctggcg cttctactcc aagcaagcga agggcttctt tgagaacgac
    35041 tgcggttgcg tagctgaggg ctagacggct gtcgcgttct gggctttcat ccaagatacg
    35101 tgtatgtgca tagtatttgt taaaggattg agccaggcta attgcaaatt tagcaatgat
    35161 agaaggttca aagttatctg ccgcacggtt gataatacgt gggaagtctt gaatgagttt
    35221 aatgatttcc cagctttcag tatcattcaa gctatagttg ccagctgttt ctggtttgaa
    35281 atcggctttg cgtaagatag attggatacg agcgtaggca tattgaacgt aaggtccagt
    35341 ttcaccctcg aaggatacca tagcttctag gtcgaagtcg tatccatttg tacggtcagt
    35401 tttgaggtca tagaatttaa tggctccaac cccaacagca tgtgctactt ggtctttgtt
    35461 ttctagttca ggatttttag cctcgatttg gaccttggca cggctaacag cctctgcaac
    35521 agtaggctct agcaagatga cattcccttt acgagtagag agcttcttcc cttcttttgt
    35581 aaccagacca aaaggaacgt gggtaatgtc gtcactccaa tcgtagccca tctcttgcaa
    35641 gacagctttg agttgtttaa agtgggcaga ttgctcttga ccaacgacgt agatagattt
    35701 agcaaattgg tattcatttt tacggtagag ggctgcagcc aagtcacgtg tgatatagag
    35761 agttgcacca tcagacttct tgatgagggc tggatgttca attccatatt tctcaagatt
    35821 cacaacttgg gcaccttctg attcaagaag tagtcctttt tcagaaagaa tgtctacaac
    35881 tgcatccatc ttatcattgt agaaggcttc tccgttatag ctgtcaaatt caaccttcaa
    35941 ttcattgtaa aggcggttaa attccactaa actttcatcg cggaaccatt gccaaagagc
    36001 gagagcttcc tcatctccat tttcaagttt acggaaccat tcgcgcgctt cttcatccaa
    36061 gctagggtca ttttcagctt cagcgttgat gcggacatag agtttaagga gttcatcgat
    36121 tggatgagct tttacagctt cttcgtcgcc ccattttttg taggcaacaa tcaacatccc
    36181 aaattgttta ccccagtctc ccaaatggtt gaccttgacc gtttgataac cgattttttg
    36241 gaaaatatgt gacaagctat ctccgataac agttgaacgc aggtggccaa tagaaaatgg
    36301 tttagcgata ttcggactag acatgtcgat aacaacattt tcttgtttgc caatattttg
    36361 gtcagcatag tgttcttttt cagtggtaac agcttgcaat acttgagcag aaatggcaga
    36421 tttatcaagg aaaaagttaa cgtaaggtcc tgttgcgaca actttttcaa aggcttggct
    36481 gttcattttt tcagccagtt cagccgcaat catttgtggt gctttacgtt cgacttttgc
    36541 aagagaaaaa gcagggaaag caatgtctcc catttctgag tttttagggg tttccagtaa
    36601 ctttaaaata gcctcttggt ccaggctatc aatgatgcta gataattcgc tagcaatcaa
    36661 ttcttttgta ttcattaaga gctccttttt ggacttttct actattttat cacaatttta
    36721 aagaaagaag aaaaaatttt tgaaatctcc tgtttttttg gtataatatg gttataaata
    36781 tagttataaa tatgcacgca agaggatttt atgagaaaaa gagatcgtca tcagttaata
    36841 aaaaaaatga ttactgagga gaaattaagt acacaaaaag aaattcaaga tcggttggag
    36901 gcgcacaatg tttgtgtgac gcagacaacc ttgtctcgtg atttgcgcga aatcggcttg
    36961 accaaggtca agaaaaatga tatggtgtat tatgtactag taaatgagac agaaaagatt
    37021 gatttggtgg aatttttgtc tcatcattta gaaggtgttg caagagcaga gtttaccttg
    37081 gtgcttcata ccaaattggg agaagcctct gttttggcaa atattgtaga tgtaaacaag
    37141 gatgaatgga ttttaggaac agttgctggt gccaatacct tattggttat ttgtcgagat
    37201 cagcacgttg ccaaactcat ggaagatcgt ttgctagatt tgatgaaaga taagtaaggt
    37261 cttgggagtt gctctcaaga cttatttttg aaaaggagag acagaaaatg gcgatagaaa
    37321 agttatcacc cggcatgcaa cagtatgtgg atattaaaaa gcaatatcca gatgcttttt
    37381 tgctctttcg gatgggtgat ttttatgaat tattttatga ggatgcggtc aatgctgcgc
    37441 agattctgga aatttcctta acgagtcgca acaagaatgc cgacaatccg atccctatgg
    37501 cgggtgttcc ctatcattct gcccaacagt atatcgatgt cttgattgag cagggttata
    37561 aggtggctat cgcagagcag atggaagatc ctaaacaagc agttggggtt gttaaacgag
    37621 aggttgttca ggtcattacg ccagggacag tggtcgatag cagtaagccg gacagtcaga
    37681 ataatttttt ggtttccata gaccgcgaag gcaatcaatt tggcctagct tatatggatt
    37741 tggtgacggg tgacttttat gtgacaggtc ttttggattt cacgctggtt tgtggggaaa
    37801 tccgtaacct caaggctcga gaagtggtgt tgggttatga cttgtctgag gaagaagaac
    37861 aaatcctcag ccgccagatg aatctggtac tctcttatga aaaagaaagc tttgaagacc
    37921 ttcatttatt ggatttgcga ttggcaacgg tggagcaaac ggcatctagt aagctgctcc
    37981 agtatgttca tcggactcag atgagggaat tgaaccacct caaacctgtt atccgctacg
    38041 aaattaagga tttcttgcag atggattatg cgaccaaggc tagtctggat ttggttgaga
    38101 atgctcgctc aggtaagaaa caaggcagtc ttttctggct tttggatgaa accaaaacgg
    38161 ctatggggat gcgtctcttg cgttcttgga ttcatcgccc cttgattgat aaggaacgaa
    38221 tcgtccaacg tcaagaagta gtgcaggtct ttctcgacca tttctttgag cgtagtgact
    38281 tgacagacag tctcaagggt gtttatgaca ttgagcgctt ggctagtcgt gtttcttttg
    38341 gcaaaaccaa tccaaaggat ctcttgcagt tggcgactac cttgtctagt gtgccacgga
    38401 ttcgtgcgat tttagaaggg atggagcaac ctactctagc ctatctcatc gcacaactgg
    38461 atgcaatccc tgagttggag agtttgatta gcgcagcgat tgctcctgaa gctcctcatg
    38521 tgattacaga tgggggaatt atccggactg gatttgatga gactttagac aagtatcgtt
    38581 gcgttctcag agaagggact agctggattg ctgagattga ggctaaggag cgagaaaact
    38641 ctggtatcag cacgctcaag attgactaca ataaaaagga tggctactat tttcatgtga
    38701 ccaattcgca actgggaaat gtgccagccc actttttccg caaggcgacg ctgaaaaact
    38761 cagaacgctt tggaaccgaa gaattagccc gtatcgaggg agatatgctt gaggcgcgtg
    38821 agaagtcagc caacctcgaa tacgaaatat ttatgcgcat tcgtgaagag gtcggcaagt
    38881 acatccagcg tttacaagct ctagcccaag gaattgcgac ggttgatgtc ttacagagtc
    38941 tggcggttgt ggctgaaacc cagcatttga ttcgacctga gtttggtgac gattcacaaa
    39001 ttgatatccg gaaagggcgc catgctgtcg ttgaaaaggt tatgggggct cagacctata
    39061 ttccaaatac gattcagatg gcagaagata ccagtattca actggttaca gggccaaaca
    39121 tgagtgggaa gtctacctat atgcgtcagt tagccatgac ggcggttatg gcccagctgg
    39181 gttcctatgt tcctgctgaa agcgcccatt taccgatttt tgatgcgatt tttacccgta
    39241 tcggagcagc agacgacttg gtttcgggtc agtcaacctt tatggtggag atgatggagg
    39301 ccaataatgc catttcgcat gcgaccaaga actctctcat tctctttgat gaattgggac
    39361 gtggaactgc aacttatgac gggatggctc ttgctcagtc catcatcgaa tatatccatg
    39421 agcacatcgg agctaagacc ctctttgcga cccactacca tgagttgact agtctggagt
    39481 ctagtttaca acacttggtc aatgtccacg tggcaacttt ggagcaggat gggcaggtca
    39541 ccttccttca caagattgaa ccgggaccag ctgataaatc ctacggtatc catgttgcca
    39601 agattgctgg cttgccagca gaccttttag caagggcgga taagattttg actcagctag
    39661 agaatcaagg aacagagagt cctcctccca tgagacaaac tagtgctgtc actgaacaga
    39721 tttcactctt tgatagggca gaagagcatc ctatcctagc agaattagct aaactggatg
    39781 tgtataatat gacacctatg caggttatga atgtcttagt agagttaaaa cagaaactat
    39841 aaaaccaaga ctcactagtt aatctagctg tatcaaggag acttctttga caattctcca
    39901 cttttttgct agaataacat cacacaaaca gaatgaaaag gagctgacgc attgtcgctc
    39961 ccttttgtct attttttaag gagaaagtat gctgattcag aaaataaaaa cctacaagtg
    40021 gcaggccctg gcttcgctcc tgatgacagg cttgatggtt gctagttcac ttctgcaacc
    40081 gcgttatctg caggaagtct taggcgccct ccttactggg aaatatgaag ttatttatag
    40141 tatcggggct tggttgattg gtgtggccgt agtcggtcta gttgctggtg gactcaatgt
    40201 tgtcctcgca gcctatattg cccaaggagt ttcatccgac cttcgggagg atgccttccg
    40261 taaaattcaa accttttctt atgctgatat tgaacaattt aatgcgggaa atctagtcgt
    40321 tcgaatgaca aatgatatca accagattca gaacgttgtc atgatgacct tccaaattct
    40381 tttcagactt cccctcttgt tcatcggttc gtttatccta gcggttcaaa ccttaccttc
    40441 tctgtggtgg gtgattgttc tcatggtagt cttgattttt ggtttgactg ctgtcatgat
    40501 gggaatgatg gggcctcgtt ttgctaaatt tcaaaccctt cttgagcgca tcaatgccat
    40561 tgccaaggaa aatttacgtg gcgttcgtgt ggtcaagtcc tttgtccaag aaaaagagca
    40621 atttgctaag tttacagagg tctcagacga gcttcttggt caaaaccttt acattggtta
    40681 tgccttttca gtagtggaac cctttatgat gttggttggt tacggggcgg tcttcctctc
    40741 tatttggctg gtcgcgggaa tggttcagtc ggatccgtct gttgttggtt ccatcgcttc
    40801 ttttgttaat tacctaagcc agattatctt taccattgtt atggttggat ttttgggaaa
    40861 ttctgtcagc cgtgccatga tttccatgcg tcgtattcga gaaattcttg acgcagagcc
    40921 agctatgacc ttcaaggata tcccagatga agagttggtt ggaagtctta gctttgaaaa
    40981 tgtgaccttt acctatccaa tggacaagga accgatgctg aaagatgtga gctttactat
    41041 tgaacctggt caaatggttg gtgtagttgg agcgactggt gcaggaaagt caaccttggc
    41101 tcaattgatt ccacgtctct ttgatccaca ggacggggcc attaaaatcg gtggcaagga
    41161 tattcgagaa gtgagtgaag gaaccctgcg taaaacagtt tccatcgttc tccaacgtgc
    41221 cattcttttt agtggaacga ttgcagataa cttgagacag gggaagggga atgctactct
    41281 atttgaaatg gagcgcgcag ccaatattgc ccaggctagt gaattcattc atcgtatgga
    41341 gaaaaccttt gaaagtccag ttgaagaacg gggaaccaat ttctctggtg gacaaaaaca
    41401 aaggatgtcg attgcgcgtg ggattgtcag caatccacgt attctgattt ttgacgattc
    41461 gacctcagcc ttggatgcca aatcagagcg cttggtgcaa gaagctttga ataaggactt
    41521 gaaggggacg acaaccatta ttattgctca aaaaattagc tcggttgtcc atgcagacaa
    41581 gatcttggtt ctaaatcaag gacgattgat tggtcaaggc acgcatgcag acttggttgc
    41641 caacaatgcc gtttaccgtg aaatctatga aacacagaaa tgaaagacaa actataagaa
    41701 aagtcaatag ttttatctaa actatttctt atttcaattt gatgatttgg cgatgatttt
    41761 agagcacggc aaaaagccct tgaaaaagtc cattttttca aaggtaatcc tgtgttgatt
    41821 tcagaaatta catcactttt tgttcgtcaa atggcagctc ttttttagga tataaaacag
    41881 ggttcggata agtttttttg caaggtggat gatggctaca ttgtaatgtt ttccttattc
    41941 taacttagtc ttaagatagg ccttaaaagc aggtgaaaag cgagcgcatg ctttggcagc
    42001 ttgtatgagt gcctaccgca gatgagggga accccgtttg atcattcttc cagctaaatc
    42061 aatctgacct gactgataaa tagaagaatc cagtccagcg aaagcttgta attgagcagg
    42121 attatcaaag gcatgaatat tttgaatctc ggctaaaatg accgccccta aacgattccc
    42181 aatcccagta accgtcgtga tgaccgaatt taactcagcc atcaagtcat tgacacattt
    42241 ttccgccttg tcaatgagcc tcttgtaatg tttgatgttt tcattacacg agataaaacg
    42301 tctatgcgtt atcaaactca ttaccaatta aaacaaatgt ggttagatcc tttcggaaat
    42361 tgtcaagcga ttggaggaaa tgaactaatc cacagcggct tattccaagt ataccacttg
    42421 ggctttggca gtagctaact gcgctaaata taatataagg aggagtaaaa tgaagacagt
    42481 tcaatttttt tggcattatt ttaaggtcta caagttctca tttgtagttg tcatcctgat
    42541 gattgttctg gcgacttttg cccaagccct ctttccagtc ttttctggac aagcggtgac
    42601 gcagctagcc aatttagttc aagcttatca aaatggcaat ccagaacttg tatggcaaag
    42661 cctatcagga atcatggtca atcttggcct gctggttttg gttctattta tctctagtgt
    42721 aatatacatg tgtctcatga cgcgcgtgat tgcagaatcg accaacgaga tgcgcaaagg
    42781 tctctttggt aagcttgctc agttgacggt ttctttcttt gaccgtcgac aagatggcga
    42841 tatcctgtct cattttacca gtgatttgga taatatcctc caagccttta acgaaagctt
    42901 gattcaggtc atgagcaata ttgttttata cattggtctg attcttgtca tgttttcgag
    42961 aaatgtgacg ctggctctca tcaccattgc cagcacccca ttggctttcc ttatgctgat
    43021 tttcatcgtg aaaatggcac gcaaatacac caacctccag cagaaagagg tagggaagct
    43081 caacgcctat atggatgaga gcatctcagg ccaaaaagcc gtgattgtgc aaggaattca
    43141 agaggatatg atggcaggat ttcttgaaca aaatgagcgc gtgcgcaagg caacctttaa
    43201 aggaagaatg ttctcaggaa ttcttttccc tgtcatgaat gggatgagcc tgattaatac
    43261 agccatcgtc atctttgctg gttcggctgt acttttgaat gataagtcta ttgaaacaag
    43321 tacagcccta ggtttgattg ttatgtttgc acaattttca cagcagtact accagcctat
    43381 tatccaagtt gcagcgagtt ggggaagcct tcagttggcc tttactggag ctgaacgaat
    43441 tcaggaaatg tttgatgcag aggaggaaat ccgacctgaa aaggctccaa ccttcactaa
    43501 gttgcaagaa agtgttgaaa tcagtcatat cgatttttca tacttgcctg ataaacctat
    43561 tttgaaagat gtcagcattt ctgcccctaa aggccagatg acagcagttg ttgggccgac
    43621 aggttcagga aaaacgacta ttatgaacct catcaatcgc ttttatgatg ttgatgctgg
    43681 tggtatttat tttgatggta aagacattcg tggctatgac ttagatagtc ttagaagcaa
    43741 ggtgggaatt gtattgcaag attcggtctt gtttagcgga acgattcgag acaatatccg
    43801 atttggtgtg ccagatgcta gtcaggaaat ggttgaggta gcagcaaaag caacccacat
    43861 tcacgactat atcgaaagtt tgcctgataa gtacgatact cttattgatg atgaccagag
    43921 catcttttca acagggcaga agcaattgat ttcaatcgct cgaaccctga tgacagatcc
    43981 agaagttctc attctcgatg aagcaacttc aaacgtagat acggtgacag aaaacaagat
    44041 tcagcatgcc atggaggtgg ttgtagcagg tagaactagt ttcgtcattg cccaccgctt
    44101 gaaaaccatt ctcaatgcag atcagattat tgtccttaaa gatggagaag tcattgaacg
    44161 tggtaaccac catgaacttt tgaagctagg tggcttttat tcagaactct atcacaatca
    44221 atttgttttc gaataagaaa gaagttgtcc tatgtgggca gctttttctt gtccataaaa
    44281 aatgtttatc acagccttaa aaaaaacata ttagacgaaa gtcattttga gtgatatgat
    44341 aggactatcg ttagcattcg aaaggagagg catcatggct agaacggttg taggagttgc
    44401 tgcaaatcta tgtcccgtag acgcagaagg caaaatcatt cattcatctg tatcttgtag
    44461 attcgcagag atcattcgtc aaatcggtgg tctcccttta gtcattcctg ttggtgatga
    44521 gtcagttgta cgtgattatg tggaaatgat tgacaaactc attttgacag gaggccaaaa
    44581 tgttcatcct cagttttatg gagagaaaaa gaccgtcgag agcgatgatt acaatctggt
    44641 ccgtgacgaa tttgaattgg cactcttgaa ggaagcgctt cgtcagaata aaccaattat
    44701 ggcaatctgt cgcggtgtcc aacttgtcaa tgttgccttt ggtggaaccc tcaatcaaga
    44761 aatcgaaggt cactggcaag gactaccttt cgggacatct cactctattg agacagtaga
    44821 aggaagcgtg gtggctaagc tatttgggaa agaaagtcag gtcaactcag tccatcgtca
    44881 aagcattaaa gatttggcac ctaatttccg tgtaactgct attgattcaa gagaccaaac
    44941 tattgaagcg attgagtcta tcgacgagca ccgcattatc ggtttacagt ggcatccaga
    45001 gtttctggtt aatgaagaag atggcaattt agaattattt gagtatttat tgaatgaact
    45061 gtaacgattg gaacatctgg tcgttctttc ttttattttt tcaaaatttt tagaatgtgg
    45121 cattttacgc aaacgtttga attctgataa aaattggaga aattcgacaa aaaaacttga
    45181 aaaaaacgaa ggtaagcgtt atgatagaaa agaagaaata ttggaggaag aacatgtcac
    45241 atattaaatt tgattattca aaagttttag acaaatttgt tgcaccacat gaagtggaat
    45301 acatgcaatc acaagtaaca gcagcagatg aattgatccg taaaggaact ggtgctggta
    45361 gcgacttctt gggatggttg gaccttcctg aaaaatacga tcgcgaagaa tttgaccgca
    45421 tcttgaaagc tgctgagcaa atcaagtcag acagcgatgt tttggttgtg atcggtattg
    45481 gtggatctta ccttggtgca aaagcagcaa tcgacttctt gaatcaccat tttgctaact
    45541 tgcaaacaaa agaagaacgc aaagctccac aaatccttta cgcaggaaac tcaatctcat
    45601 ctacttacct tgctgacttg gtagagtatg ttgcagacaa agacttctca gtaaacgtaa
    45661 tttctaaatc aggtacaaca actgaaccag cgattgcttt ccgtgtcttt aaagaactct
    45721 tggttaagaa atacggtcaa gaagaagcta acaaacgtat ctatgcaaca actgaccgcc
    45781 aaaagggtgc tgttaaggtt gaagcagacg ctaacggttg gggaacattt gttgttccag
    45841 atgatatcgg tggacgcttc tcagtattga cagccgttgg tttgcttcca atcgcagcat
    45901 caggagctga catcaaagct cttatggaag gtgcgaatgc agctcgcaaa gactacactt
    45961 cagacaaaat ctctgaaaac gaagcttacc aatacgcagc tgttcggaac atcctttatc
    46021 gtaaaggcta tgcaactgag atcttggtaa actatgagcc atcacttcaa tacttctcag
    46081 aatggtggaa acaattggct ggtgaatcag aaggaaaaga ccaaaaaggt atctacccaa
    46141 cttcagccaa cttctcaact gacttgcact cacttggtca atttatccaa gaaggaactc
    46201 gtatcatgtt tgaaacagtt gtccgtgttg acaaacctcg taaaaacgtg cttattccta
    46261 ctttggaaga agaccttgac ggacttggtt accttcaagg aaaagacgtt gactttgtaa
    46321 acaaaaaagc aactgacggt gttcttcttg cccacacaga tggtgatgta ccaaacatgt
    46381 atgtgactct tccagagcaa gacgctttca ctcttggtta cactatctac ttcttcgaat
    46441 tggcaattgc cctttcaggt tacttgaatg ctatcaaccc atttgaccaa ccaggtgttg
    46501 aagcttataa acgtaacatg tttgcccttc ttggaaaacc aggatttgaa gaattgagca
    46561 aagaacttaa cgcacgtcta taatagaaga aaagagtggt ttgcccactc tttttactct
    46621 ctttatccat agaaattgga ctcagccaag acttgtgata taatatagaa agcaaaaagg
    46681 cagacgccta gataatagga gaaactatgt caaaagatat ccgcgtacgt tacgcaccaa
    46741 gtccaacagg actactacac atcggaaatg ctcgtacagc attgtttaat tacttgtatt
    46801 cgcgccatca tggtggaaca tttctcatcc gtatcgaaga tactgaccgt aaacgccatg
    46861 tcgaggatgg tgaacgttca caacttgaaa accttcgctg gttaggcatg gattgggatg
    46921 aaagtccaga atcacatgag aattatcgcc agtctgagcg tttggacttg tatcaaaaat
    46981 acattgacca actattagct gaaggaaaag cctataaatc ttacgttaca gaagaagagt
    47041 tggcagctga acgcgaacgc caagaagtag ctggcgaaac accacgctac atcaatgaat
    47101 accttggtat gagtgaagaa gaaaaagcag cttacatcgc agaacgtgaa gcagcaggga
    47161 tcatcccaac tgttcgtttg gctgtcaatg agtcaggtat ctacaagtgg catgatatgg
    47221 tcaaaggcga tatcgaattt gaaggtggca atatcggtgg tgactggatt atccaaaaga
    47281 aagacggtta cccaacttac aactttgccg ttgccatcga tgaccatgat atgcaaatct
    47341 ctcatgttat ccgtggagac gaccatattg ctaacacacc aaaacagctc atggtttatg
    47401 aagcacttgg ttgggaagct ccagagtttg gtcacatgac cttgattatc aactctgaga
    47461 ctggtaagaa gttatctaaa cgtgatacta atacacttca atttatcgaa gattaccgaa
    47521 agaaaggtta ccttccagaa gcagtcttta actttattgc tcttcttggt tggaacccag
    47581 gtggtgaaga tgagattttc tctcgtgaag aactcattaa actttttgat gaaaaccgcc
    47641 tcagcaagtc accagcagcc tttgatcaga aaaaactaga ctggatgagc aatgattata
    47701 tcaagaatgc agaccttgaa accatctttg aaatggcaaa accattctta gaggaagcag
    47761 gccgtttgac tgacaaggct gaaaaattag ttgagctcta taaaccacaa atgaaatcag
    47821 tagatgagat tatcccattg acagatcttt tcttctcaga tttcccagaa ttgacagaag
    47881 cagagcgcga agtcatgacg ggtgaaacag ttccaacagt tcttgaagca ttcaaagcaa
    47941 aacttgaagc gatgacagat gataaatttg tgacagaaaa tatcttccca caaattaaag
    48001 cagttcaaaa agaaacaggt attaaaggga aaaatctttt catgcctatt cgtatcgcag
    48061 tttcaggcga aatgcatggg ccagaattac cagatacaat tttcttgctt ggacgtgaaa
    48121 aatcaattca gcatatcgaa aacatgctaa aagaaatctc taaataaaga ggatacaata
    48181 atggacatct gggaaaagat gtacgaagaa gcacagaaac tatacaatcc acatgaagta
    48241 tcagactttg tttatgccaa tcatgttgta gctgcagtag aagcagaaga tggacaaata
    48301 tttacaggat tctgtatgga gggaacctgt ggtgttttcc atctctgcgc agaacgggcg
    48361 gccctcttca atatgtacca attttcagga caaactaagg ttaagaaagt attagccttt
    48421 cgagacaaac caccttatgg tggaagttca gccatgcctt gcggtgcttg tagagaattc
    48481 cttttagagc ttaatgctga aaataaagat gcagaattca tgatggacta taatataaga
    48541 aaaacagtta aagtcgcaga actaatccct tattggtggg gagaagaacg tgcttctaag
    48601 tttaatgagc gatagaagag tcagtataat tctggctttt tttactataa gttgaaaaaa
    48661 taaaaaaaat ta
//
LOCUS       DAVLRT010000005        48296 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLRT010000005 DAVLRT010000000
VERSION     DAVLRT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.005"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            58..1950
                     /gene="pepO"
                     /locus_tag="VXM85_000268"
     CDS             58..1950
                     /gene="pepO"
                     /locus_tag="VXM85_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1344902.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVATVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2073..2702)
                     /locus_tag="VXM85_000269"
     CDS             complement(2073..2702)
                     /locus_tag="VXM85_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1344903.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3698..5920
                     /locus_tag="VXM85_000270"
     CDS             3698..5920
                     /locus_tag="VXM85_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1344904.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5950..6393
                     /locus_tag="VXM85_000271"
     CDS             5950..6393
                     /locus_tag="VXM85_000271"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1344905.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGVAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGANMQVELVNNGPVTIILDTKKR"
     gene            complement(6432..6847)
                     /locus_tag="VXM85_000272"
                     /pseudo
     CDS             complement(6432..6847)
                     /locus_tag="VXM85_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7206..7568
                     /locus_tag="VXM85_000273"
     CDS             7206..7568
                     /locus_tag="VXM85_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344906.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEEDVLILLSELQNFFLDVFQAKRIHRA
                     FCVRLCLKAQFLLSNNDS"
     gene            7749..8048
                     /locus_tag="VXM85_000274"
     CDS             7749..8048
                     /locus_tag="VXM85_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344907.1"
                     /translation="MNRDVLEFLRTETAEKISLYISEANRLEGDVTLLAPSSQDLEDI
                     KNAMLSNSNLGLKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(8143..8580)
                     /locus_tag="VXM85_000275"
     CDS             complement(8143..8580)
                     /locus_tag="VXM85_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344908.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(8682..9347)
                     /locus_tag="VXM85_000276"
     CDS             complement(8682..9347)
                     /locus_tag="VXM85_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV1344909.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVGISMLSTRF
                     TGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMNLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIEGNSN
                     KEY"
     gene            complement(9535..10185)
                     /locus_tag="VXM85_000277"
     CDS             complement(9535..10185)
                     /locus_tag="VXM85_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1344910.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            10297..11139
                     /locus_tag="VXM85_000278"
     CDS             10297..11139
                     /locus_tag="VXM85_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1344911.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQENFDRLVTHPPCDIAVY
                     GHIHQQLLRHGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDGKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            11215..11652
                     /locus_tag="VXM85_000279"
     CDS             11215..11652
                     /locus_tag="VXM85_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1344912.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            11721..12023
                     /locus_tag="VXM85_000280"
     CDS             11721..12023
                     /locus_tag="VXM85_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344913.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFTFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(12114..13187)
                     /locus_tag="VXM85_000281"
     CDS             complement(12114..13187)
                     /locus_tag="VXM85_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1344914.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            13343..14020
                     /locus_tag="VXM85_000282"
     CDS             13343..14020
                     /locus_tag="VXM85_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1344915.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            14013..14987
                     /locus_tag="VXM85_000283"
     CDS             14013..14987
                     /locus_tag="VXM85_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1344916.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLFLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(15036..16979)
                     /gene="thrS"
                     /locus_tag="VXM85_000284"
     CDS             complement(15036..16979)
                     /gene="thrS"
                     /locus_tag="VXM85_000284"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1344917.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNIAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVNYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(17037..17360)
                     /locus_tag="VXM85_000285"
     CDS             complement(17037..17360)
                     /locus_tag="VXM85_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1344918.1"
                     /translation="MELQELVEHSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            18225..18770
                     /locus_tag="VXM85_000286"
     CDS             18225..18770
                     /locus_tag="VXM85_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1344919.1"
                     /translation="MKLAVIAANGQVGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAES
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            18996..19265
                     /gene="rpsO"
                     /locus_tag="VXM85_000287"
     CDS             18996..19265
                     /gene="rpsO"
                     /locus_tag="VXM85_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1344920.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            20081..20695
                     /locus_tag="VXM85_000288"
     CDS             20081..20695
                     /locus_tag="VXM85_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1344921.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(20988..21737)
                     /locus_tag="VXM85_000289"
     CDS             complement(20988..21737)
                     /locus_tag="VXM85_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1344922.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMILKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            21870..24206
                     /locus_tag="VXM85_000290"
     CDS             21870..24206
                     /locus_tag="VXM85_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1344923.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            24307..24984
                     /locus_tag="VXM85_000291"
     CDS             24307..24984
                     /locus_tag="VXM85_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1344924.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVPHVSK"
     gene            24971..25768
                     /locus_tag="VXM85_000292"
     CDS             24971..25768
                     /locus_tag="VXM85_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1344925.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            25785..26888
                     /locus_tag="VXM85_000293"
     CDS             25785..26888
                     /locus_tag="VXM85_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1344926.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWLSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLSILDQAPANELFPGLQGFDNLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            26895..27086
                     /locus_tag="VXM85_000294"
     CDS             26895..27086
                     /locus_tag="VXM85_000294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344927.1"
                     /translation="MKIKEQTRKLAADYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            27189..28208
                     /gene="galE"
                     /locus_tag="VXM85_000295"
     CDS             27189..28208
                     /gene="galE"
                     /locus_tag="VXM85_000295"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1344928.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGIIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            28210..29175
                     /locus_tag="VXM85_000296"
     CDS             28210..29175
                     /locus_tag="VXM85_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1344929.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(29226..29432)
                     /locus_tag="VXM85_000297"
     CDS             complement(29226..29432)
                     /locus_tag="VXM85_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1344930.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            29484..29960
                     /locus_tag="VXM85_000298"
     CDS             29484..29960
                     /locus_tag="VXM85_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1344931.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            29969..30640
                     /locus_tag="VXM85_000299"
     CDS             29969..30640
                     /locus_tag="VXM85_000299"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1344932.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            30770..31108
                     /locus_tag="VXM85_000300"
     CDS             30770..31108
                     /locus_tag="VXM85_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1344933.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIHIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            31111..31740
                     /locus_tag="VXM85_000301"
     CDS             31111..31740
                     /locus_tag="VXM85_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1344934.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            31980..33131
                     /locus_tag="VXM85_000302"
     CDS             31980..33131
                     /locus_tag="VXM85_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1344935.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGARLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            33449..34198
                     /gene="truA"
                     /locus_tag="VXM85_000303"
     CDS             33449..34198
                     /gene="truA"
                     /locus_tag="VXM85_000303"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1344936.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQIDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            34188..34961
                     /locus_tag="VXM85_000304"
     CDS             34188..34961
                     /locus_tag="VXM85_000304"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1344937.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            34939..35400
                     /locus_tag="VXM85_000305"
     CDS             34939..35400
                     /locus_tag="VXM85_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1344938.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(35659..35862)
                     /locus_tag="VXM85_000306"
     CDS             complement(35659..35862)
                     /locus_tag="VXM85_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1344939.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(35855..36937)
                     /locus_tag="VXM85_000307"
     CDS             complement(35855..36937)
                     /locus_tag="VXM85_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1344940.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(37063..37845)
                     /locus_tag="VXM85_000308"
     CDS             complement(37063..37845)
                     /locus_tag="VXM85_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1344941.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(37845..39188)
                     /locus_tag="VXM85_000309"
     CDS             complement(37845..39188)
                     /locus_tag="VXM85_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1344942.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGTYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDVQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(39287..40603)
                     /locus_tag="VXM85_000310"
     CDS             complement(39287..40603)
                     /locus_tag="VXM85_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1344943.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQNLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDINGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            40796..42022
                     /locus_tag="VXM85_000311"
     CDS             40796..42022
                     /locus_tag="VXM85_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1344944.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYILWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            42221..43795
                     /locus_tag="VXM85_000312"
     CDS             42221..43795
                     /locus_tag="VXM85_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1344945.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            44059..44847
                     /gene="codY"
                     /locus_tag="VXM85_000313"
     CDS             44059..44847
                     /gene="codY"
                     /locus_tag="VXM85_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1344946.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGQLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            44847..45422
                     /locus_tag="VXM85_000314"
     CDS             44847..45422
                     /locus_tag="VXM85_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1344947.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            45504..45589
                     /locus_tag="VXM85_000315"
     tRNA            45504..45589
                     /locus_tag="VXM85_000315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45538..45540,aa:Leu,seq:aag)
     gene            45872..47002
                     /gene="ugpC"
                     /locus_tag="VXM85_000316"
     CDS             45872..47002
                     /gene="ugpC"
                     /locus_tag="VXM85_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1344948.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(47124..47294)
                     /locus_tag="VXM85_000317"
     CDS             complement(47124..47294)
                     /locus_tag="VXM85_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344949.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEYKEHHNIDA"
     gene            47501..48259
                     /locus_tag="VXM85_000318"
     CDS             47501..48259
                     /locus_tag="VXM85_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1344950.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
BASE COUNT        14101 a         8980 c        10910 g        14305 t
ORIGIN      
        1 cattccctag tgatttttgt taagataaat gcaaatacaa atgaaagcga gaacaagatg
       61 acacgttatc aagatgattt ttatgatgct atcaatggag aatggcaaca gacagctgaa
      121 atcccagcag ataagtctca aactggaggt tttgttgatt tagaccagga aattgaagac
      181 ctgatgctgg cgacaacaga caagtggtta gcaggtgaag aagtgcctga ggatgctatc
      241 ttggaaaact ttgtcaaata ccaccgccta gttcgtgatt ttgacaagag agaagctgac
      301 ggtatcacac ctgtcttacc actccttaaa gaattccaag aattggaaac ttttgcggat
      361 tttacagcta aactagcaga gtttgagctt gcaggaaaac caaacttcct tccttttggt
      421 gtatcgccag actttatgga tgctagaatc aatgttctat gggctagcgc tccaagcaca
      481 atcttgccag atacgaccta ctatgcagaa gaacatcctc agcgcgaaga gctcttgact
      541 ctttggaaag aaagcagcgc aaatctcctc aaggcttatg atttctctga tgaagaaatt
      601 gaagacttgc tagaaaaaag acttgaattg gaccgccgag ttgcgacagt ggtgctctct
      661 aatgaagaaa gttcagaata tgctaaactc tatcatccat attcttacga agatttcaag
      721 aaattcgcgc ctgccctacc tttggatgac ttcttcaaag cagttattgg gcaattacca
      781 gacaaggtta ttgtagacga ggaacgtttc tggcaagcag cagagcaatt ctacagtgag
      841 gaagcctggt ctctccttaa agcaaccttg attttgagtg ttgtcaatct ttcaaccagc
      901 tatttaacag aggatatccg tgttttgtct ggtgcctaca gccgtgccct ttctggagtt
      961 ccagaggcaa aagataaggt caaagcagct tatcatctag cacaggaacc tttcaagcaa
     1021 gccctggggc tttggtacgc ccgtgagaag ttctctccag aagccaaggc ggatgtggag
     1081 aaaaaagtgg caaccatgat tgatgtctat aaggagcgtc tgcttaagaa tgactggctc
     1141 actccagaaa cctgtaaaca ggctatcgtg aagctcaatg tgatcaaacc ttatattggc
     1201 tatccagaag aattgcctgc acgttacaag gataaggtag tgaatgaaac tgccagtctt
     1261 tttgagaatg ctctagcctt tgcgcgtgtg gaaatcaagc acagttggag taagtggaac
     1321 cagcctgtag actataagga atggggcatg cctgctcata tggtcaatgc ctactacaat
     1381 cctcagaaga acctgattgt ctttccagcg gccattttac aggcgccttt ctatgacttg
     1441 catcagtcat cttctgctaa ctacggtggt attggggcag tgattgccca tgaaatttcc
     1501 cacgcctttg atactaacgg ggcttccttt gacgaaaatg gtagcctcaa ggattggtgg
     1561 acagagagcg actatgctgc cttcaaggag aaaacacaaa aagtcattga ccaatttgat
     1621 ggacaggatt cttatggagc aaccattaac ggtaaattga ctgtatcaga aaacgtggct
     1681 gacttgggag gaatcgcagc agcgcttgaa gcagctaaga gagaagcaga cttctcagca
     1741 gaagagttct tctacaactt cggtcgcatc tggcgcatga aaggtcgtcc agaatttatg
     1801 aaacttttgg ctagcgtcga tgtgcacgca ccagccaaac tccgtgtcaa tgtgcaagta
     1861 ccaaacttcg acgatttctt tacaacctat gatgtcaaag aaggagacgg aatgtggcgt
     1921 tcaccagagg agcgcgtgat tatttggtaa tacaaaaaat ctagtcatca gaaaaaaggc
     1981 atccaatcac gtgtgaaaac cttgatagga tgccttttgt aatagaaaga tttgctggat
     2041 agtttactta aatttcgatt gtcatcatct tgctagaaaa agggattgaa ggccttttcg
     2101 tgagcgatag tagtagctgg accatgtcct ggatagacat cgtagtttgg tagggtgaag
     2161 agttgggtct ggatactatg aaggagttgc tccatgctac cagtcggaag gtcggtccgt
     2221 ccgatagttt cgcggaatag agcatctccc gtcaagacta ggtgagcatc aggaaagact
     2281 agggaaacac caccgataga gtgccctggg gtcggtagaa ccttaaaacg aaattcctct
     2341 agttggtatt cttcgtgaaa gacaaaggtg tgttctgcag gttttgtgac cacatctgcc
     2401 atatcatcgt ggcgagggag accggagaga ttatcgacag gagtgtagag ccagctggct
     2461 tcgctctctg cgatatagac aggaggattg ccaaacgtct cgcgaaccaa gtccagactc
     2521 atgatatggt cataatgggc gtgggtcaag agaatagcac agatcggttt gttgatcttc
     2581 tcgattgtct gacgaatggc ttcccaatga ctaccaggat cgacgacgat gaggtgcttt
     2641 tcgccttcta gataataggt attttcatag gcaacaggat tcacggtttt atggattttc
     2701 atactagctc caatctcaaa gaatgtacta atactcttcg aaaatctctt caaaccgcgt
     2761 caactttatc tgcaacctca aagcagtgct ttgaacagcc tgcggctagc tttctagttt
     2821 gctctttgat tttcattgag tataaattaa gtatagcaca gttagggaga ataggtaagg
     2881 atttaaaatg aacttaaaaa ccagcatgac tagatgaaga caggctggtt atcaatttat
     2941 caatattgga aaggtgtcaa ttgcccttca taggttgcat aaactccgtc attagcttgt
     3001 ttgatagacg tgatgtttag agtgctaccg tagttggctt ctcatttgtc gctatattca
     3061 ctataagtta tcctactggg caggttctgg tcgttagcat agtattgaac gactgttttt
     3121 tatagttttg ctggctgaat aggaacaggc agcaaactcc taacattagg agagcaaaga
     3181 tagaaatagc agtttttttc ataggtattc tccttataaa ctaaaggata gaagaaagta
     3241 tcttgaggat ttttatactc ttcaaaaatc tcttcaaacc gcgtcaacgt cgccttgccg
     3301 tatatatgtg actgactttg tcagtcttat ctacaacctc aaagcagtgc tttgaacagc
     3361 ctgcggctag tttcctagtt tgctcttgga ttttcattga gtattaattc caaaatccag
     3421 tcaaggtacc agtgtatgtg atacgagttc caccgtttat ctttcgtatc ttcaatggaa
     3481 ttggggaaat cagaaagtat ttgggttaca atatggtttc cgttcttcta tctctccttc
     3541 tatcacctaa gtttaaaatc taacttcgta gtatattatg ggccaataat tcagaattat
     3601 caagcaattt tgaattattt ttttcttatt ctgttccaat ctagttttca tattcttaaa
     3661 aaagtgataa aatggtagga agaattggag agtagagatg ccgaaagaag tgaatttaac
     3721 aggcgaagaa gttgtcgctt taaccaaaga atatttaacg gaagaggatg ttcattttgt
     3781 ccataaggcc ttggtctatg ctgttgaatg ccacagtggt caatatcgca aatcaggcga
     3841 gccttatatc attcacccta tccaagtggc aggtatttta gctaagctaa agctggatgc
     3901 tgtaacagta gcttgtggat tcttgcatga tgtggtggaa gatacagatg cgaccttgga
     3961 cgatttggaa agagagtttg gtcctgatgt gcgggtgatt gttgacggag ttaccaagct
     4021 tggcaaggtc gagtacaaat cgatcgagga gcaattagcg gaaaatcatc gcaagatgct
     4081 catggccatg tctgaggaca tccgcgttat tttggtcaaa ctgtctgacc gcttgcacaa
     4141 tatgcggacc ctgaaacatc ttcgaaaaga caagcaggag cgtatttcca aagaaaccat
     4201 ggaaatctat gccccacttg cccatcgttt ggggatttcc agtgtcaaat gggaattaga
     4261 agacttgtct ttccgttatc tcaatccaac ggagttttac aagattaccc atatgatgaa
     4321 ggaaaagcgc agggagcgtg aggccttggt ggatgaggta gtcacaaaat tagaggagta
     4381 tacgacagaa cgtcacttga aagggaagat ttatggtcgt cccaagcata tttactcaat
     4441 tttccgcaaa atgcaggaca agagaaaacg gtttgaggaa atctatgatc tgattgctat
     4501 tcgttgtatt ttagataccc aaagtgatgt ttatgccatg cttggttacg tgcatgaatt
     4561 ttggaaaccg atgccaggtc gcttcaaaga ctatattgcc aaccgcaagg ccaatggtta
     4621 tcagtctatc catacgactg tttatggacc aaaagggccg attgaattcc agattcgaac
     4681 caaggaaatg cacgaggtgg ctgagtacgg ggttgcggct cactgggctt ataagaaagg
     4741 tataaagggg caagttaaca gcaaggaatc agctattgga atgaactgga tcaaggagat
     4801 gatggagctc caagaccagg ctgatgatgc taaggaattt gtggactctg ttaaggaaaa
     4861 ctatttggct gaggagattt acgtttttac cccagatgga gctgtccgtt cccttcccaa
     4921 agattcagga ccgattgatt ttgcctacga aatccatacc aaggtcggtg aaaaagcaac
     4981 tggtgccaag gtcaatggcc gcatggttcc actgacaacc aagttaaaga caggggatca
     5041 ggttgaaatt atcgccaacc cgaactcctt tggacctagc cgtgactggc tcaatatggt
     5101 caagactagc aaggcgcgca ataagattcg ccagttcttt aaaaaccaag ataaggaatt
     5161 gtctgtcaac aagggtcgtg agatgctgat ggctcagttc caagaaaatg gctatgtggc
     5221 aaataaattt atggacaagc gccacatgga tcaagttctg caaaagacca gttacaagac
     5281 agaagactcc ctctttgcgg ccattggttt tggggaaatc ggtgcgatta ccgtctttaa
     5341 ccgtctgact gaaaaggagc gccgtgagga agagcgtgcc aaggccaagg ctgaggcaga
     5401 ggagcttgtc aaaggtggcg aggtcaaggt tgaaaataaa gaaactctca aggtcaagca
     5461 tgagggggga gtggttattg aaggtgcttc tggtctccta gtgcggattg ctaagtgttg
     5521 taaccccgtg cctggtgacg atattgttgg ctacattacc aagggtcgtg gtgtggctat
     5581 tcaccgtgtg gactgtatga acctgcgtgc ccaagaaaac tacgagcaac gtctccttga
     5641 tgtggaatgg gaagaccagt actctagctc aaataaggag tatatggccc atatcgatat
     5701 ctacggtctc aaccgtacag gactgttgaa cgatgtactg caagttcttt caaatacaac
     5761 caagaatatt tcaacggtca atgcccaacc aaccaaggat atgaagtttg ctaatatcca
     5821 tgtgtccttc ggtattgcca acctctctac actgactacg gttgtcgata aaattaagag
     5881 tgtgccagaa gtttactctg tcaaacggac caacggctag ttgtcctagc tcttactaga
     5941 aaggctatta tgaaaatcat tatccaacgg gttaaaaaag cccaagtgag tatagaaggc
     6001 cagattcagg gaaaaatcaa tcagggactt ttattgctgg ttggtgttgg accagaggac
     6061 caagaggaag atttggacta tgctgtgaga aaactggtca atatgcgaat tttttcagac
     6121 gcagaaggca agatgaacct atctgtcaaa gatattgaag gagaaatcct ctctatttct
     6181 cagtttaccc tctttgcgga tactaagaaa ggcaatcgtc cagcctttac aggggtagct
     6241 aaacctgata tggcatcaga cttctatgat gctttcaatc aaaaattagc gcaagaagtg
     6301 cccgttcaga caggtatctt tggagcaaat atgcaggttg agctggttaa taacggacct
     6361 gttaccatta tcctagatac taaaaagaga taagaaagac caagcccagt cggcttggtc
     6421 tttctcatct atcataaaat actccaaaaa gaaatcggtt cttgatatgc ttgggggaag
     6481 tctcttttca ggctttggca gatgcgatag gaagggatga gatgtcctag gatgaggaga
     6541 gttccctgaa ggaaaagtgg aatatcactt aaagacacca aggagagaat catcaggcta
     6601 tcccatagga gaatttgtaa agcaaaacgc cagaatctcg gtctaatctg ggaatagagt
     6661 tgactaaagt ggtcacaaag ttggtttgaa agataggtca atagcacagg atgaaagaaa
     6721 atgagccaac cgctataaat caaaatccag tcccaagtaa gccccccttt ttaaatgtca
     6781 aaaatgccag catgattcca tcaatgacaa ggagttgaat ggttttgatg aagatgattt
     6841 ttttcatgct aaaacctcct tttccttaga gacactcccc tacaggtatt ttagaatgta
     6901 tattgcttag atattcctta ttttattagc tttttaaggt attaaagtag ttatataagt
     6961 ttatgaaggg tatcctatcc tgcacggcta attttgagtt cgaaactgag gagtttataa
     7021 ctcaaaattt cgattaaatc agcatttaac gatagtatgt ttgtcataaa atgataaatt
     7081 tttagattgt ggaaattttt tttaacttaa ttcttgattt tttttctaaa tgtgatatac
     7141 ttaatttacg ggataaaaaa accaccttag aaaggtgtga taatgtgcaa aaagagaatg
     7201 ttagaatgcg tttagcgtct gaatccagac agatattaga taagcttaaa gatgatgttt
     7261 acaataaatt aggctatgaa gtaagctatt ccagtattgt tagtcaagct gttagagaat
     7321 acgttcctaa aaaagaacgt atagattgga taaaattaaa agagacagct ataccctttt
     7381 catcgttaaa acagagcaat aattgggagt accagacatc acttatgtta gaggaagatg
     7441 tattaatctt actgtctgaa ctacaaaatt ttttcttaga tgtcttccaa gcgaaacgta
     7501 tacatagggc attttgtgta cgcctatgct tgaaagcaca atttttatta agtaataatg
     7561 attcttaata ctatcaatat taatgcggag tgtaatgctc cgctaaaata aaacctatta
     7621 cgggataaaa acactacttt atttaggatg gaaagaggaa cgaatgaaga tgaaagctag
     7681 agattcacca ttgaattgat tttgttttta gaaaaaatca ataattagaa aaaatacgag
     7741 gtaaaaaaat gaatagagat gtattagagt ttctgagaac agagaccgct gaaaaaatta
     7801 gtctttatat aagcgaggct aatagattgg agggcgatgt aactctactc gcacctagtt
     7861 ctcaagattt ggaagatatt aaaaatgcta tgctttccaa ttctaatctc ggattaaaag
     7921 ttgcgagact ggatgtgatg aagaaaatag catatgcttc tacaagaaat cattatttaa
     7981 caggggctac gatttttggg gatatttcga aaggaacata caattgtgat cctaaaagtt
     8041 atgtctaata taagggaggt taatccctcc ccttgttttt gaaaatcgaa aggaaaagta
     8101 taaaaatgaa agttaatgaa tatttctgga gaacgagctg tcttatacta aaaaagaaga
     8161 aatcgataaa atagtatacg tgttagagat gtgtttgtcg taaaagtctg gatagttttc
     8221 tcctaggtag tcagctgtca tataataaaa agtagaatgg caagcagtga taattggcat
     8281 aagagatgaa aaacgctgag aactaatgaa ggctaagaga acaaaaaata gaaaatcaat
     8341 ggtgagaacc cctaaatagt agaagcgaat ctttttattg atttgaggat aaatctcagc
     8401 gaattgatgt ttgagtcgga cgaccaagag aaatagcagg agtccttgag caaggaagaa
     8461 aattaaacca gagagcaaga gccactcaaa agatgtctcg agtctagtga taaaaaatgc
     8521 aaatagtagc aaatcaataa ctgcaattgc tataaaatgg attctataga gttttttcat
     8581 gacaagacct tcctctttta tctaacttca ttctactcca aaagaatggg agttacaact
     8641 aaaatgataa aaatagcaga agggagattc tcttaagttg gctagtattc tttatttgag
     8701 tttccttcta tgtatctaac ttcttcatca ttccagacaa ataaagctcc gattgcattg
     8761 aggatataaa agatgtattt accgatattg gcgaagtttc cttgaatacc agcttttgtc
     8821 agctgaacga aattgtaaat caaccaaaag ccccactgag ttgttagttt caatgcattc
     8881 aaagcattgg caatgaggga cagtgcaaag gcaatagttg ttacgtaggc aaggagattc
     8941 atcttgccct catatccgat atagttggtc acaaaggcaa agaggaaggc gatgatggaa
     9001 atgatgatgg ccgccaattt tacctgtttt tggctcattt ggttgggtct gccttcttgc
     9061 gaagctttcc atttctttat agcaaaggta taaatgagga aggtgacggg ataggtaatg
     9121 atggccgcct tatttccaag gatataatca atagcaccgg acaaaatggt attaacaata
     9181 ccaaagtaat ttccccattt gcttaatttc cccgtgaaac gagtggacaa catggaaatc
     9241 ccaacgttgg ttacggaaat caatccaaag ggtacaagag ctgtccatgg tccccagtct
     9301 acaaatttat cgaggcgtga gttgaggtaa ccagatgcaa tcgcaatcca acgaccaaag
     9361 caaccccgaa gaggtcaaac tatttagatg tagcaaaaat ttttagtgat tttttcatag
     9421 gttaaactac ctttcttttt ttcaaatatt ctcccaccaa atgaaagtaa aataaaatga
     9481 tagaaataaa accctgaaaa taaaactttc agggttggta ggggacttga gaaattagtt
     9541 gattttctcg acatagagtt gttttgcaat gtccatattc acttgtaaat cctcgtcgtt
     9601 actgaggata gtgaaggtat tgctgaagcc atcaaactgc ttgacttgga gctggtcacc
     9661 gatgtgaagt gagtgcttgt ccagataatg gagaatgtca aaactatcgt gcacccgagt
     9721 caggcggtag gcgccagctt ccttgatatc agctagtggg aggttattga tttcaacgag
     9781 gagttctccc ttggcaggaa tagttcctcc gtgggggcag gttttaggga aacctagcag
     9841 tttatctagt ctttccacga acaggtcaga gacagtgtgt tccaagacct cagcttcctc
     9901 gtgaatctgg tcacttgtat agtctaaatg atgaactaga aaaacttcaa tcaagcggtg
     9961 cttacgatag agctcagaga ccagtttgag accgaggtca gtcagtagat agccacattc
    10021 cttgtccttt aggatgagat tttcactttt cattcgtttg atcatttcag ttacggcagg
    10081 gggagagact tgcatgcgag ccgcaatttc cttgttggta atcttatgca ggtctatgcc
    10141 aatttcataa atacatttta gatagtcttc tttgtttggg gtcattcgtt tcctcttgtc
    10201 taatatcttt atctagtata tcaaaaaaag ctagatttct ctagctgtga ctttttatgt
    10261 tatacttatt gcaagagaaa aaacgaggag atggatatga cgaaaatagc tcttctttca
    10321 gatattcatg gaaataccac cgccttggag gctgttttgg cagatgctca gcagctagga
    10381 gtggatgaat actggctttt gggagatatt ctcatgccag ggacaggacg tagaaggatt
    10441 ttggacttgt tggatcaact accgattacg gctagagttt tgggaaactg ggaagacagt
    10501 ctttggcatg gtgtccgtaa ggaattggat agtactcgcc ctagtcaacg ctatctcttg
    10561 cgccagtgcc agtatgtttt agaggaaatt tccctagaag aaattgaagt gctccacaat
    10621 caacctcttc aaattcatcg tcagtttggg gatttgacag tgggaattag ccatcatctg
    10681 cctgataaga actgggggcg agagttgatt catactggca aacaagagaa ttttgaccgc
    10741 ttggtgactc atcctccctg tgatattgct gtttatggac atattcacca gcagttgctt
    10801 cgtcacggga ctggtgggca attgattgtc aatccgggtt cgattggcca acctttcttt
    10861 ttggacgctc agctacggaa ggacttgcgg gcccagtata tgattttgga gtttgatggc
    10921 aagggcctgg tagatatgga cttccgacgg gtagactacg atgtggcagc tgaattgcag
    10981 ctggctaaag acctgagact tccctatttt gaggtttact atgaaagtct ggtcaatggg
    11041 atccaccata ctcatcatca ggaatttttg agagaattgg ctcagaaaga gggttgcgat
    11101 cgggagttag acgactggtt gaaaagtggt aacgattgac attacaacta aaaagggtat
    11161 aataatagaa aaagaagagt agaggcaggg tagcctcgta aaaaggagaa gaggatgcaa
    11221 attccaagta gatttaccat tgcgactcat atgctgataa tcattgccct cgaggggaag
    11281 gaaagcaagg tgaccagtga ttttctggct gctagtgtcg gggtcaatcc tgtcattatc
    11341 agaaagatct tgtcccagtt gaagaaggca gagctgattt cagtagcgcg tggaacgggc
    11401 ggaacagaga ttgtcaagga ccttaaggat attagtcttt tagatgttta tcaggcggtc
    11461 gaatgtcttg gtaagacagg tcaactcttc agtttccatg acaatccgaa tccaaattgc
    11521 cctgtaggag ctcatattca tgatgttttg gatcaaaaat tggagagaat tcagttgact
    11581 atggaggcag aacttggtca gaccagtcta gaaaaagtcg tggccgatgc agagagtcag
    11641 atgaaggatt aagagggata aatgccctct ttttttcttt ggataattat gataaaatgt
    11701 agctattaaa ggagtaagaa atgtatctcg ttatagaaat tgtattagcc tttatagtgt
    11761 cattttggaa ggataataga agtttgtgga atccagtatt atttttatta tccctcattt
    11821 caagttattt ttatctatcc tacctttttt ataaaaatgg atacgagaat gttcagttag
    11881 ctttttatgt atttaccttt gtcttacttc cttttttgct ttttctaagt ggcatctttt
    11941 taatctataa tggggtcatt ttgttgaaac gagagggacg ttccaagccc cattatctct
    12001 caatgctctt tgatttttat tgactattag attctattta aaaagactta gaatgcacat
    12061 gaaagtatgt attctaggtc ttttgttttg tatacattta attttcaaga gtattatcta
    12121 tttttccatg catggtaacg agtatcaatc aataactggg taaggcgacc caaggtttct
    12181 ctttcttctg gagtgaggga ttgagcttgg acaaagagat gatgaatgta ttcaagcgcc
    12241 tttaaggaag ccaagtcatt gatagagcta ataccagcat caagaatagt gccaaagaag
    12301 aggtcgaagt agagctgaag ttcttcgtca gggactgtgg ccacccattc tttaaaggtt
    12361 gtgtctactt gccggctatc actgttggtc ttatccagtt gaacgaagtg cttgtcctca
    12421 atctgccaac taaaggtatc gtgctgggcg atgccaccca gggcagtact ctgaacgatg
    12481 atttggtgag caggaatttc cagcatcata ccgataatag acccttgtgg aatgaatatc
    12541 ttgcttctat ccattatcct ttgataaccc gcagtctgtg tcaattcttg atggagacca
    12601 ggtgcatcaa atgtataaac tgctgtgatc tgattttgca aactttgctc aatttggcta
    12661 gcagcataga tagcgagatt tcctcccttg gaatgcccag ccagaataac cttttgctta
    12721 ggatgatggg caaaaaagtt ctttaaatag cgaagggcgt gcttttgagc aggaatttcc
    12781 ttcatatagg tcaggtggaa atcttccttc cagccaatga tactgtcatc tgtcccacga
    12841 aagacaatca gataggtatc gaggctgaca cgataagtca tagccgcaaa ttgcttttgc
    12901 agttcagggt cgatgtcgtt gataaaatgg gagagtttgc aatttttgaa gcgcttgtgt
    12961 tgagccaatt catctaataa ttgaaggcga tttttgctag taagcatggt gggctctctt
    13021 ggaacctgag gtgctagatc taaaagacgt tgaggaagtg tggagaccag attatcaaag
    13081 gagaggtagg tgatttctat taaggttaga atgtctaact cattcaaggg aaggtcgtaa
    13141 taagaatcat atgcgacatc tttcagatag tcaaaaatat tggccataag agctcctttc
    13201 tttttattta tcttagcaaa tttccctcaa attagctagt agcatagcct gtttgtactg
    13261 gctaaaaaca ggctatttca aattcagttt cagaccatct agcatggaaa aatctgttat
    13321 aataatggaa aaggagaagc gcatgcacaa gattttatta atagaagatg atcaggtcat
    13381 tcgtcaacag attgggaaaa tgctctctga atggggattt gaagtggtcc tggtagaaga
    13441 ctttatggaa gttttgagtc tatttgttca gtcggaacct catctggtcc tcatggatat
    13501 tggtttgccc ttgtttaatg gttatcactg gtgtcaggaa atccgcaaga tttccaaggt
    13561 acctatcatg tttctttctt cgagagacca ggctatggat attgtcatgg caatcaatat
    13621 gggggcggat gactttgtga ccaagccttt tgaccagcag gttcttttag ctaaggttca
    13681 gggcttgttg cgtcgttcct atgagtttgg gcgtgatgag agtttgctgg aatatgctgg
    13741 tgttatcctc aataccaaat ccatggattt acattatcaa gggcaagtct tgaatttgac
    13801 caagaatgaa ttccagattt tacgcgtgtt atttgagcat gcaggcaaca tcgtagcacg
    13861 tgacgacctg atgcgggaac tttggaacag tgactttttc attgatgata ataccctctc
    13921 tgtcaatgtg gctcgtttgc gtaaaaagtt ggaggagcag ggattggtag gatttatcga
    13981 gaccaagaaa ggaatagggt acggattgaa gcatgcttga ttggaaacaa ttttttctag
    14041 cctatctgcg ctcccgtagt cgtcttttta tctatctgct ttttttggca tttcttgtct
    14101 tactctttca gtttttattt gccagtctag gaatttactt cctctacttt ttcttcttgt
    14161 gttgctttgt aaccatctta tttttcactt gggacatatt ggtggaaacg caggtctatc
    14221 gccaggaact tctctatgga gagagggaag ccaagtctcc tttggaaata gctttagcag
    14281 aaaaattaga agcgcgtgag atggaactct atcagcagag gtcaaaagca gaaagaaaac
    14341 tgacggattt gctggattac tataccttgt gggttcatca gataaagacc cccattgcag
    14401 ccagtcaact cttagttgca gaagtggtcg accgccaact gaagcagcag ctagaacagg
    14461 aaattttcaa aatcgactcc tataccaacc tagttttaca gtacctgcgt ttagaaagtt
    14521 tccatgatga tttggtctta aagcaggttc aaattgagga cttggtcaag gaaataattc
    14581 gtaaatatgc tcttttcttt attcaaaaag gcttaaatgt caatctacat gaccttgata
    14641 aagaaatcgt gacggataaa aagtggctgc tagtggttat tgagcaaatc atctcaaaca
    14701 gtctcaagta caccaaggaa ggtggtctgg agatttatat ggatgaccaa gagctttgta
    14761 tcaaagatac gggaatcggg ataaaaaaca gtgatgtcct ccgagtattt gaacgtggct
    14821 tttcaggata caatggccgt ttgacccagc agtcctctgg acttggcctt tatctatcta
    14881 agaaaatttc tgaagaactg gggcaccaga ttcgtatcga gtctgaggtc ggaaaaggaa
    14941 cgacagtgcg gattcagttt gctcaagtga acttagtcct tgagtaaaaa tagatgagaa
    15001 aaagcctcca gattggaggc ttttatgcta gactcttatt tctcaacgcg tgatttgttg
    15061 gcgatatcag ctaggatagc ttgaacaaaa ttatcaactg agacagtttg tgtttctttt
    15121 tggccgtagc gacgaacgtt gactgtttcg tcttccattt ctttgtctcc aacaattaat
    15181 tggtaaggaa tcttgctggt ttgtgaagca cggatcttga actgcatttt ttcattgcgc
    15241 tcatctacgt ctgcacggac accgcggtca cggagtttct tggccacttc ccaagcgtag
    15301 ttcacgtgtt tttcgttaga tactgggatg agggttactt ggtgtggtgc cagccatgtt
    15361 gggaaggccc ccttgtagtt ctcaatcaag atagctgtga agcgttccat agttgagata
    15421 accccacggt ggatcatgac tggacggtga tcttcgccat cagctccgat gtatttgagg
    15481 tcgaagcgtt ctggcaacaa gaaatcaagt tggatagtag aaagggtttc ttcttttcca
    15541 agggcagttt taatctggat atccaatttt ggtccgtaga aggctgcttc accttcggct
    15601 tcaaagtagt ccacgcccat ttcatcaaga gctgcacgaa gcatggtttg ggcattttcc
    15661 cacatctcat cgttatcaaa gtacttatga gtatcttgag ggtcacgaag agagaggcgg
    15721 aagcggtagt cagtcaagtt gaagtcttca taaacatcga taatcaactg aagggcacgt
    15781 tggaattctt cttggatttg ttctggagta acgaataggt gaccgtcgtt gagtgacatt
    15841 tcacgtacac gttgaaggcc agtgagggca ccagattttt cgtaacggtg catcataccg
    15901 atttcagcga tacggattgg caattcacgg taagagtgaa catggtgttt gaaaacttgg
    15961 atgtggtgcg gacagttcat tggacgaagg acaaattctt ccccgtcacc catgtccatg
    16021 gttgggaaca tgtcttcttg gtaatgatcc cagtgaccag aagtcttgta aagctcaaca
    16081 gaagcaagtg gtggagtgta gacgtgttgg tagccagaag ccaactcttt gtttacgatg
    16141 tagcgttcca attcacgacg gatagtcgca ccatttggca accagaatgg caaaccttgt
    16201 cccacttctt gtgaaatcat aaagaggtca agctctttac caagtttacg gtggtcacgt
    16261 tccttagctt cttcacgcat ttgaaggtag tttttcaagt ctttcttgtc aaaccaagct
    16321 gtaccgtaga tacgttgcat catagcgttg tcgctgtttc cacgccagta agcaccagct
    16381 acatggagaa ggtggaagat ttggatacga cctgttgatg gaacgtgagg tccacggcag
    16441 aggtctacat attcaccctg acgatagata gtcaaaccgc cttcgtcttc tgagtgttct
    16501 tcaatcaatt ccaacttgta agggtcattt ttgaagattt cacgtgcctc gtctttagtc
    16561 acttcttcac gaatagatgg gaagttttct ttgacgattt tttgcatttc ttcttcgata
    16621 cgaggaaggt cttcgttaga gatttgacca gctatgttgt cagtatcgta gtagaaacca
    16681 tcttcgatgg ctggaccaac tcccaagtga atgtctggga aaagacgacg agctgcttgg
    16741 gcgaacaagt gagctgctga gtgacgcaag attggaaggg catcttcgtg atcaggtgtc
    16801 acaatttcga tgcttccatc ttcagtgata gcgcgagtag tgtcgatgag tttgccgttg
    16861 aatttaccag ccaaggcttt tttagctagg gaattgctga tagattgggc aatttcaaaa
    16921 gttgttacgc cagattcgaa ttcacgaaca gcgccatctg ggaaagtaat gttaatcatg
    16981 atgttctcct tttttattat ttcatagttt ctaagcattg attttttatc agcagactac
    17041 ttccaagtcc taacgttcaa ttgcttttct ttatcagaga ggaagttttc catttccgtc
    17101 agaatgtcta tatccaaacg ttgagaaagt tctaatagcc accagatatt ttctgaaagt
    17161 ttttgttcca gtgtgtaggg tgtttcatca tagtagcgtc cttgctttgt catcaccaat
    17221 cgttggaaat ttccaatatc attagataaa gccaagaggt cttcttctac cgtccacttg
    17281 gaatcatgat gcttaacttc cagttcgtga taagcttgtc ggattgccca actgtgctcc
    17341 actaattctt gcaactccat aattagtatc ctaataaaaa aatgcgacat cctcgaaagg
    17401 acgtcgcaac gtggttccac cttcatttat gtacctcaaa agagagggac acctctgatc
    17461 ggctctaacg tggccaccgt tttatgtttt cataaaaaac tcaagagtag tatcagttta
    17521 gacttcctat gcgtttccag caaccacgca ctctctagta gaaagggact aagtgacttg
    17581 tctctatgga ttattatagc atgattgtga gatttttcaa ggattttgtg aaagtttttt
    17641 gtttttcatt ttttgtagaa tatgcttgat gctaatacca gaatccatgc ccaccagagc
    17701 aactgtttca tagattagca agtaaattag tataaactca ccaaagatta tgatagtgaa
    17761 gtaaaagagc agtccgttac caactaacca tcaaagtgag aaacggaaac ggtagctata
    17821 gagtaacgat acaatcaaga ttaccagagg tgtaaagaag agaagattgt ttatatagag
    17881 tcccttgagt gtaagggggt cagaaacaac tagcaagaac aactcataaa gtggccaata
    17941 aatatgacca gatagtaggg gagatgggaa tagaatctac gcatgataat cacctcgttt
    18001 taaaaaaaac agacaactta gatctcttgc tatgattatt ataagcatgt ttaaaacaag
    18061 aaagtaaatt tgttgacaaa gaagattttt cttttcacta gaatcatagt tagttttctt
    18121 ggtagaaata ttttgataat aattaagcaa gactattgac attgattatt aagagtgtca
    18181 caattgctgt aactaaaata attacaacaa gagaggtata aaaaatgaaa ctagcagtta
    18241 ttgcagcaaa tggacaagtg gggaaagcaa ttgttgaaga agcagtcaaa cgaggacatg
    18301 aagtaacggc tattgttcgt tctgaaaaca aaagtcaagc tgagtctatt attaaaaaag
    18361 atttatttga gttgacaaaa gatgatctta ctggatttga tgctgttatt tcagcgtttg
    18421 gtgcttatac accagatacg ctaccgctcc atagcaaatc aatcgaacta tttaatcagc
    18481 ttcttgctgg aacacagaca cgttttctag ttgtaggggg agcagaaagt ctttatatag
    18541 atgaaacaaa aacgacacga cttttagata cgtctgattt tcctgaagaa tttaagtcac
    18601 tagcaaaagc tcaagcggat gaattggatc ttcttcgtac aaaaaataat ctcaactgga
    18661 cttttgtcag tcctgctgta gattttattc ccgatggtga aaaaacagga aactacattc
    18721 tagcaggaga aatttttact actaatgaaa aaaggaataa gccagattag ttatgcagat
    18781 tatgctattg gattggtaga tgaacttgaa aaaggtcatc atataaaaga acgtatctct
    18841 ttacttgaaa aataataact atgagagtat taatgtttga cagaaaattt aaatcttggt
    18901 aaaataggga ttgtcgtaaa gacaaataac ttcttcttgg ttacaggcat gccaacctgt
    18961 cactcggatg aagccaaata aaaaggagaa acatcatggc aatctcaaaa gagaaaaaaa
    19021 atgaaatcat tgcacaatat gcacgtcacg aaggtgatac aggttcagta gaggttcaag
    19081 ttgctgtcct tacttgggaa atcaaccacc ttaacgaaca catcaaacaa cacaaaaaag
    19141 accacgctac ttaccgtgga ttgatgaaaa aaatcggtcg tcgtcgtaac ttgcttgcat
    19201 acttgcgtaa aaacgacgtt aaccgttacc gtgagttgat caactctcta ggacttcgtc
    19261 gctaattcaa gatacaaagg ccgtcaaaag cacaaagcaa aaataggaaa attgacgaag
    19321 aaacttcagt ttctaggaga ttttatcttt tttgccaagt gcttaggccg tgttcaattg
    19381 agcatatctt gataataaag ctactctaaa ttgggtagct tttttgcgtt gtcaccttac
    19441 ctcgctatac tcaagtataa tctttggtta cggttcctag cactgtaagg taaaataaac
    19501 cagcttttct cccttcgggg agattttttt tgtttcactg aaagtttcgt acgatcttcg
    19561 cctcgtcgcc tagtctataa acgatttatc tagcaagaat catgatgcta agggcgtcaa
    19621 aaatccgtat gaaaataggg aaatgacaca gtgttcgatg agcacaagga gtttcatctt
    19681 tttcactagg attttagccc gggctaaaat cagctctctg acttcagagg gcttttttat
    19741 tgaggtttta tcggtaacaa ttttggagac tacaaaaacc tcaaatggta tcagctaatt
    19801 acaccataaa ggtgcgcagt tactcggctt ttcaagccga gtagctgtat gcaagccccc
    19861 tagaagagcc cactctttac aaagtaaagt atagtatgtt atactttaca tggaagtagt
    19921 cactgaattc cagttagaaa ttactttgta actacgtttt gaggaggagt aaaatgcttt
    19981 cctacgttcg acattaccca ctagcgatag ctaaattaat gtgtctgtgc tctcctgaaa
    20041 tctgctgatt tattactgac taatacagga ggtttttttt atgggacaga caatcatatc
    20101 tgctattggt gtttatattt ccaccagtat cgattattta attattttaa ttattttatt
    20161 tgcacagcta tcacagaata aacagaaatg gcatatttat gcggggcaat atctaggcac
    20221 aggcttactt gtaggggcga gtttagttgc tgcttatgtc gttaatttcg tgcctgaaga
    20281 atggatggtt ggattgcttg gtttaatccc tatctattta gggattcgct ttgcaattgt
    20341 tggagaagat gcggaagaag aagaggaaga aattattgaa agattagaac aaagcaaggc
    20401 aaatcaactg ttttggacag ttacattgct gacaattgcg tctggcggag ataatttagg
    20461 tatctatata ccttattttg cttcgttaga ttggtcacag accctcgtgg ccttgcttgt
    20521 gtttgtaatc ggcataatta tcttttgcga gattagtcgg gtgttatcct ctattccgtt
    20581 aatattcgag acaattgaaa aatacgagcg aatcattgtg cccttagtat tcattctact
    20641 tggactatac atcatgtatg aaaatggcac gatagagact tttctgatcg tgtagatttt
    20701 tttgtttcac taggatttta gcccgagctc aaatcagctc tctgattttc agagggcttt
    20761 ttcattgagt ataaatggac aatcttaaag ttactctatt gtagagtggc ttttgatttg
    20821 attaggagat tcttataaat atagtaaaat gaaacaaaaa caggacaaat cgatcaggac
    20881 agtcaaatcg atttctaaaa atgttttaag aagtagaggt gtactattct agtttcaatt
    20941 tgctatataa atgaaaacct tagctgtttg gccaaggttc atttcttcta tgcaagttct
    21001 tctcttttct tatctgggtt ccagatgaag cttgcgataa agctaaagat gatggttagg
    21061 atagcgagga taatagcaag gatgagggca gggatgcgga taaaccacca gagtggattt
    21121 ggttttttat cattgtgcca ttgtttcgtt tcttcgtcca gacgttggaa ttctgtttga
    21181 atacgattgg cgactgtttc aatgccttca tcattcattt tcttgatatc tgagatatcg
    21241 attggatttc caaagttcat atcgacacgt tcacggctaa tcaagccctt caaaatcatg
    21301 ggaccggtgt aggtaaccgg catgatacgg accttggcca ttttggcaat cagtgctgcg
    21361 ccccccttga catcgtttga gtggcggcta ccacttggaa acatgatgag agagcggtca
    21421 ctttttttga gaacgttgat aggatatttg atggctgagg cgctgggatt ttcacggtcg
    21481 atgggaaagg cgccacacat acgaatccac caaccaaaga tacggttggt aaagagttct
    21541 ttttttgcca taaagatgaa ctgttttggc ttggtcgcaa aggccatata aacaggatcc
    21601 caccaggtac ggtgaggcgc aactaaaata taattttcat cttgattagg aattttatca
    21661 gtattatgat agtgagcatt gccattgatg gaccataaga gcaacacaac taatccacgc
    21721 aaataagtat aaaacatgcg atctccttcg attgttttct tgttattatt ataccttatc
    21781 aaaggagggc tggcaaactt ttcccttgac tagatgcata tttgggatga aattagaatt
    21841 ctgttaaaaa aaatggtata atagaattta tggataaaaa taagattatg ggattaaccc
    21901 aaagagaagt caaggaaaga caggctgagg gtttggtcaa tgactttacc gcatcagcca
    21961 gtaccagcac ttggcaaatc gttaaacgaa atgtctttac cctttttaac gctttgaact
    22021 ttgccattgc tttggccctt gcctttgtgc aggcttggag caatctggtc ttctttgctg
    22081 ttatctgctt taacgctttt tctgggattg tgaccgagct acgagccaaa cacatggtgg
    22141 acaagctcaa tctcatgacc aaggaaaagg tcaaaaccat ccgtgatggt caggaagttg
    22201 ctcttaatcc tgaagaatta gtgctaggag atgtcattcg tttgtctgca ggagagcaga
    22261 ttcctagtga tgccttggtt ttggaaggct ttgcggaagt caatgaagcc atgttaacgg
    22321 gagaaagtga tttggtgcaa aaggaagttg acggcttact tttgtcagga agtttcctag
    22381 ccagtgggtc agttttatct caagttcacc atgtcggtgc agacaactat gctgccaaac
    22441 tcatgcttga ggctaagacc gttaaaccca tcaactcccg tatcatgaaa tcgctggaca
    22501 agttggctgg ttttactggg aagattatca ttccctttgg tctggctctc ttgctggaag
    22561 ccttgctttt aaaaggcctg cctctcaagt catccgttgt aaactcgtcg acagctcttt
    22621 tgggaatgtt gcctaaggga attgcccttt tgaccattac ttcgctcttg actgcagtga
    22681 ttaagttggg cttgaaaaag gtcttggtgc aggagatgta ctctgttgag accttggcgc
    22741 gcgtggatat gctctgtctg gacaagacgg gcaccatcac ccaaggaaag atgcaggtgg
    22801 aggctgttct tccactgacg gaaacttacg gtgaagaggc tattgccagc atcttgacta
    22861 gctacatggc ccatagtgag gataagaatc caactgccca agccattcgc cagcgttttg
    22921 tgggagatgt tgcttatcct atgatttcca atcttccctt ctcgagcgac cgcaagtggg
    22981 gggctatgga gttagaaggc ttggggacag ttttcttagg ggcacctgag atgttgctgg
    23041 attctgaagt cccagaagcc agggaggcct tggagagagg atcacgtgtc ttggtcttag
    23101 ctctcagtca ggagaaatta gaccatcaca aaccacagaa accatctgat attcaggctc
    23161 tagccttgct ggaaatcttg gaccccattc gagagggagc agcagagacg ctggactatc
    23221 tccgttctca ggaggtggga ctcaagatta tctctggtga caatccagtt acggtgtcca
    23281 gcattgccca gaaggctggt tttgcggact atcacagcta tgtagattgc tcaaaaatca
    23341 ccgatgagga attgatggcc atggcggagg agacagctat tttcggacgt gtttcccctc
    23401 atcaaaagaa actcatcatc caaacgttga aaaaagcggg tcatacaacg gctatgacag
    23461 gggacggggt taatgatatc ttggcccttc gtgaggcgga ttgttctatc gtgatggcgg
    23521 agggggatcc agcaacccgt cagattgcca atctggttct cttgaactca gactttaatg
    23581 atgttcctga gattctcttc gagggtcgtc gcgtggtcaa taacattgcc cacatcgccc
    23641 cgattttctt gataaagacc atctattcct tcctgttagc agtcatctgt attgccagtg
    23701 ctttactagg tcggtcagag tggattttga ttttcccctt cattccgatc cagattacca
    23761 tgattgacca gtttgtggaa ggtttcccac cattcgttct gacttttgag cgaaatatca
    23821 aacctgttga gccaaatttc ctcagaaaat ccatgcttcg tgccctacca agcgctctca
    23881 tggtcgtctt cagcgtcctg tttgtgaaaa tatttgggag tagccaaggt tggtctgagt
    23941 tagaaatctc aactctactc tattatctct tggggtcaat tggtttctta tccgtattta
    24001 gagcctgcat gccatttacc ctatggcgtg tcctcttgat tgtttggtca gtaggaggtt
    24061 tcctagccac agctctcttc ccaagaattc aaaaactgct tgaaatttca accttaacag
    24121 gacaaacatt acctgtttat ggtgtcatga tgttggtctt taccgtgatt ttcatcctga
    24181 ccagtcgtta ccaagcgaaa aaataaagaa agactgcaat ctgtggattg cggttttttt
    24241 aggtacaaga ttgccagccg aaatgtggta taataagagg taatagagtt tggaaagtga
    24301 gagaaaatga tttcaaagag attagaattg gtagcttcct ttgtgtcaca gggggctatt
    24361 ttactagatg tgggaagtga ccatgcttat ctgcctatcg agttggttga gagaggccaa
    24421 atcaaaagcg ctattgcagg tgaggtggtg gaaggtccct atcagtctgc ggttaaaaat
    24481 gttgaggctc acggcctaaa ggagaaaatc caagtccgtt tagccaatgg cttggcagct
    24541 tttgaagaga ctgaccaagt gtctgtcatt accattgctg gcatgggtgg tcgtttgatt
    24601 gctaggattt tagaagaagg tttggggaag ttagctaatg tagagcgttt gatcctccag
    24661 cccaataatc gtgaagacga cttgcgtatc tggctacagg atcatggatt ccagattgta
    24721 gcagaaagca tcttagaaga agctggaaag ttttatgaga ttttggtggt ggaagcagga
    24781 caaatgaagc tatcagccag tgatgttcgc tttggtccct tcttgtccaa agaagtcagt
    24841 ccagtatttg tccaaaaatg gcaaaaagaa gctgagaagc tagagttcgc cctcggacaa
    24901 atcccagaaa aaaatctgga agaacgtcaa gttctagtag ataagattca agctatcaag
    24961 gaggtgcccc atgttagcaa gtgaagtgat ccaagcttat gaagcctttt gccctcagga
    25021 attttctatg gagggagaca gccgtggtct gcaaattggc actttagaca agggtatcca
    25081 aagggtcatg gttgccctcg atattcgtga agagacagtg gcagaggcca ttgaaaaggg
    25141 tgtggacttg attatcgtca agcacgcgcc tatctttcgt cctatcaagg acttgcttgc
    25201 cagccgtccg caaaatcaga tttacatcga cctgattaag catgatatcg cagtttatgt
    25261 cagccatacc aatattgata tcgttgaaaa tgggctcaat gactggtttt gtcagatgct
    25321 aggaatcgag gagacgactt atctgcagga aacaggtcca gaacgtggaa ttggacgtat
    25381 tgggaatatt cagcctcaga cattttggga attggcccaa caggtcaagc aagtctttga
    25441 cctagatagc cttcgaatgg tgcattatca agagaatgat ttgcagaagc ctatttcaag
    25501 agtagcaatt tgtggtggaa gcgggcagtc tttctataag gatgctttgg caaagggggc
    25561 agatgtctat atcactggtg atatctacta ccatactgct caggatatgc tgtctgatgg
    25621 cttgttagca ttggatccag gtcactatat cgaagtgctt tttgtggaaa aaatcgcagc
    25681 actccttact caatggaaag agaaaaaagg ctgggaacta gagattttac ctagtcaagc
    25741 atcgaccaat cctttccgtc atatctagtt agaaagtgaa gacaatgaaa aaagttgcca
    25801 ttattggagc aggaattgtg ggagcaacag ctgcctacta cctctcgaga gaaagtgacc
    25861 tagaggtgac cgtttttgac catgagcaag gtcaagccac caaggccgca gcaggaatta
    25921 tcagtccttg gctttcaaaa cgccgcaata aggcctggta caagatggcg cgattggggg
    25981 ctgactttta tgtggattta ttagctgatt tagagaaatc aggacaagaa atcgactttt
    26041 accagcgttc gggagtcttt ctcttgaaaa aggatgaatc caagttggaa gaactctatc
    26101 aactggctct ccagcgcaga gaagaatctc ccttgatagg ccagttatcc attctggacc
    26161 aagccccagc taatgaatta ttccctggct tgcagggatt cgacaacttg ctctatgctt
    26221 ctggtggagc gagagtagat ggtcacctct tagtaactcg tttgctggaa gccagtcagg
    26281 tcaagttggt caaagaaaaa gtgactctga cactcttatc atcaggctac cagattggcg
    26341 aagaggtgtt tgatcaggtt attttggcaa cgggagcttg gttgggggac atgttagagc
    26401 ctttaggtta tgaagtggat gtccgtcctc aaaaaggaca actacgagat tatcagcttg
    26461 cccaagacat ggaagattac cctgttgtca tgccagaagg ggagtgggat ttgattccct
    26521 ttgcaggtgg gaaattatcc ttgggcgcta cccatgaaaa tgacatggga tttgatttga
    26581 cggtagatga acccttgctc caacaaatgg aggaggcggc cttgcctcat tacccagttt
    26641 tagctagagt gacttcaagg gctgagcgtg tgggaatccg tgcctatacc agtgatttct
    26701 ctcccttctt tgggcaggtg ccagaattgg caggtgtcta tgcagccagt ggactaggtt
    26761 catcaggcct cacaactggt cctatcattg gttaccatct agcccaactg atccaagaca
    26821 aggagttgac cttggaccct gaaaactacc caattgaaaa ctatgtcaaa cgagtaaaaa
    26881 gcgaataata ctcaatgaaa atcaaagaac aaactaggaa actagccgcg gattactcaa
    26941 agcactgctt tgaggttgtg gatagaactg acgaagtcag ctcaaaacac tgttttgagg
    27001 ttgtggatag aactgacgaa gtcagtaacc atacccatgg caaggcgacg ttgacgtggt
    27061 ttgaattgga ttttcgaaga gtataagcat tttactgaaa ttttatcagg tagtttagga
    27121 tggcaaatga cattccctgt caaaaatggt aaaataagaa aaaataatct gagaatcgag
    27181 gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc cggttttatt ggaacccaca
    27241 ctgttattga attgatccaa gcaggccatc aagttgttgt ggtggataat cttgtcaata
    27301 gcaatcgtaa gagtttagaa gttgttgaaa gaatcacagg agttgagatt cctttctatg
    27361 aggcagatat tcgtgacaca gataccctca gagatatttt caagcaggaa gaaccgactg
    27421 gtatcattca ctttgctggt ttgaaggctg ttggcgaatc aacacgtatc cctcttgcct
    27481 actatgacaa caatatcgct ggaactgtca gccttttgaa agccatggaa gaaaacaact
    27541 gtaaaaacat catcttcagt tcttctgcga cagtttacgg ggatccgcac acagtgccca
    27601 tcttggaaga tttcccactt tcagtgacca acccatacgg tcgtactaag ctcatgctag
    27661 aggaaatttt gactgatatt tacaaagcag actcagaatg gaatgttgtc ttgcttcgtt
    27721 actttaaccc aatcggagcc catgagagtg gtgatttggg agaaaatcca aacggtattc
    27781 caaacaatct cttgccatat gtgactcaag tagccgttgg aaaattagag caagtgcaag
    27841 tgtttggaga cgattacgat acggaagatg gaacaggtgt tcgtgattat atccacgttg
    27901 tcgatttggc taagggtcac gttgcagctt tgaaaaaaat ccaaaaaggt tcaggactaa
    27961 acgtttataa ccttggaact ggtaaaggtt actcagttct tgaaattatc caaaacatgg
    28021 aaaaagcggt gggacgtcct attccttacc gcatcgtaga acgtcgccca ggtgatatcg
    28081 ctgcctgcta ctcagaccca gcaaaagcta aagcagaact cggttgggaa gcagaactcg
    28141 acatcaccca aatgtgtgaa gacgcatggc gttggcaaag caagcatcca aatggatttg
    28201 aagactaaga tgatgatttc aatcatcgtc ccttgtttaa acgaagagga agtacttcct
    28261 cttttttatc aggctctgga agctttactt ccagatttgg aaacagaaat cgagtatgtc
    28321 tttgtcgatg atggatcaag tgatgggacc ttggaactct taaaggccta tcgggagcaa
    28381 aatccggcag tccattatat ttctttctct cgaaattttg gcaaagaagc agccctttat
    28441 gcaggcttgc aatatgcgac aggagatttg gtggtggtga tggatgcaga cctccaagat
    28501 cctcctagta tgttgtttga gatgaaaaat gtactagaca aaaatgtaga cttggactgc
    28561 gttgggacac ggagaactag tcgggaggga gaacccttct ttcgcagttt ctgtgctgtt
    28621 ctcttttatc gcctcatgca aaaaatcagc ccagtagctc tgccgtcggg tgtccgtgat
    28681 tttcgtatga tgagaaggtc tgtagtcgat gccattttaa gcttgaccga gtccaatcgt
    28741 ttttcaaagg ggctcttcgc ctgggtgggt ttcaaaacct actatttgga ctatccaaat
    28801 gtcgaaaggc aggctggcaa gacaagttgg agttttaggc aactcttttt ctactccatt
    28861 gaagggatta ttaatttttc agattttccc ttgattatag cctttgtagc tggtctccta
    28921 tcttgttttc tttctctgct gatgaccttt tttgttgtgg ttcggaccct cattttgggc
    28981 aatccgacat ctggttggac ctctctgatg gctgttattc tctttcttgg aggcattcaa
    29041 ctcttgacca ttgggattct cggcaagtat atcagtaaga tttatttaga gactaaaaaa
    29101 agaccacttt atcttatcaa agaaaaaagt gaccttcctg attttacaga aaaaaataaa
    29161 gtgaaaagac tataatttta catggaaatg tgctaaacta gagggagtgg attatcgcca
    29221 ttgatttagg cttcctggtt tccaatcagg gcgcgcgttg ggcaattttt aacagcctct
    29281 aagacatcct gactaggaga aatttctttt tccagttggt cagggtcatc gtaaaaacgc
    29341 acgattccat tatcgtggta atcaaataaa tcagaataag tttggcaaag cccacaggcg
    29401 atacatcgtt caggtataag tgtgattttc atgttcatat ttatattgta ataagaaaag
    29461 taaaaaaaaa caaggagtaa ggtatggcaa aagaaccgtg gcaagaagat atctatgatc
    29521 aagaagaatc aagagcagag cgtcggcatc gaaaccacgg aggggctgat aggatggcta
    29581 atcgtatttt gacgatccta gctagtattt tctttgtaat tgtggtggtg atggtcatcg
    29641 ttctcatcta tctatcatcg gggggaagta atcgcacagc agccttaaaa gactttcatg
    29701 attctgatgc aagtgtagta caaatctcat cttcaagcag ttctcagcct gagcagagtt
    29761 cagagccaga atctacttct agtagttcag aagaagctgc taatcctgaa ggaacgatta
    29821 aagttctcgc aggagaaggg gaagcagcta ttgccgctcg tgcaggaatc tccattgctc
    29881 agttagaggc cttgaatcct gggcacatgg ctacaggatc ttggtttgct aatccaggtg
    29941 atgttataaa aataaaatag gagccgatat gaaaacaatt caaattgcta ttgatggtcc
    30001 tgcttccagc ggtaagagta cggtcgcaaa gattattgct aaggattttg gattcaccta
    30061 ccttgataca ggagctatgt atcgtgcagc gacctatatg gctcttaaga accaattagg
    30121 agttgaagaa gtcgaagccc ttctagcctt gttggaccag catccaatca gctttggacg
    30181 ttcagaaact ggagaccagc ttgtttttgt aggagatgtg gatattaccc atcctatccg
    30241 tgaaaatgaa gtgaccaatc atgtttctgc tattgcagca attcctgaag tgcgtgagaa
    30301 actggtttct ctccaacaag aaattgccca gcaaggcggg attgtcatgg atggtcgcga
    30361 tattggaact gttgtattgc cacaagcaga attgaaaatt ttcctagtag cttctgttga
    30421 tgagagagca gagcgtcgtt acaaggaaaa tattgccaag ggaattgaaa cagaccttga
    30481 aaccctaaaa aaggaaattg ctgcgcgtga ctacaaggat agtcatcgtg agacttctcc
    30541 tctcaaacaa gcagaggatg ctgtctacct tgatacaact ggtttgaaca ttcaagaagt
    30601 agttgaaaaa atcaaagcag aagctgaaaa aagaatgtaa atgtagaaaa gccgatagat
    30661 tgatatcggt tttttctaaa tttgtcaaaa tgctgatttt agggtataat agttgctgtt
    30721 cgagaaattt tgattttcaa agaatagtga atgatgataa ggagaaatca tgaacaattt
    30781 accaaattgt ccaaaatgta actcagagta tgtctacgaa gacggtgccc tactggtttg
    30841 cccagagtgt gctcatgagt ggaatcctgc tgaagttgca gaagtagaag aaggtcttgt
    30901 cgctatcgat gccaacggaa ataaattggc tgatggtgat acagtaactc tcatcaagga
    30961 cttgaaagta aaaggtgcgc caaaagattt gaaacaaggg acgcgcgtga aaaatatcca
    31021 catcgtagaa ggcgaccaca atatcgactg taagattgat ggttttggtg ccatgaaact
    31081 taaatcagag tttgtgagga agatttaagc atgtcaaaac actataaact tgtattttat
    31141 agccgtatct tcttgtttct agcggctttt acgggagttt atcttgaaat tactaagcat
    31201 ggtggttttg ggatgcttct ctattacacg gttctgtcca acctcttggt aactattttt
    31261 accctttatc ttctaaaggt tatgagccgt gtaggtgaaa attggcaaag accaagtctc
    31321 ttgcgcttaa aaggtggggt caccatgagt atcatgatta cctgtgtgat ttaccatttc
    31381 ctcttggcgc ccattgcgac taatttctat accctagaaa atttcctttg ccactatatc
    31441 gttcccatct ggtttttagc ggataccctc ttttttgaca aacagggtca atacaagatt
    31501 tgggatccag cagtgtggac cattttaccc tttctgtata tgatgtttgc tctttttaat
    31561 ggcttggttc taaaactcaa tattccaaat gccaaggata atcctttccc ttacttcttt
    31621 ttgaatgtga acaagggttg gaatgttgtg tttaagtggt gtctgattat ctttgttgcc
    31681 tatatggtag caggatttat tttctacttt atcaagcaaa tcaagagaaa gtcatcctaa
    31741 tactcttcga aaatctcttc aaaccacgtc agtttccatc tgcaacctca aaacactgtt
    31801 ttgaacaacc tgcggctagc ttcctagttt gctctttgat tttcattgag tataagatac
    31861 aagggattca ttttcgaatt taagaaggag tctacaaacc aatgaggctt cttcttttct
    31921 tgcttatttg ataactatca aaaaagtaga agattaagag aaatatagaa agagatttca
    31981 tgaaacaatt tttagaacgg gccagcattt tggctctctc cctcgttttg attacctcct
    32041 tttccatttc gagtgcccta ccagccatgt ttgactatta tcagggttat tctaaggaac
    32101 aaattgagct cttggtgagc ttgccttcct ttggaatcat gatgatgtta ctgctaaatg
    32161 gtttcttaga aaaaatattt cctgagcgct tacagattag tttgggcttg ctgattttat
    32221 cattgagcgg tacagctccc ttctggtacc aagcctatcc ctttgtcttt ggagcacggc
    32281 ttctctttgg tttgggtctt gggatgatca atgccaaggc catttctatt atcagtgaac
    32341 gctaccaagg aaaaaggcga attcagatgt tagggctacg cgcttctgca gaggtcgttg
    32401 gagcttctct cattaccttg gccgtcggtc agttgttggc ctttggttgg acagctatct
    32461 ttctagccta tagtgctgga tttttggtgc tgccccttta tctgctcttt gtcccttatg
    32521 gaaaatcaaa gaaagaagtc aagaaaagag cgaaggaagc aagtcgttta actcgagaaa
    32581 tgaaaggctt gatttttacc ttagctatcg aagcggcagt tgtagtttgt accaatacag
    32641 ctattaccat ccgtattcca agtttgatgg tggaaagagg attgggggat gcccagttat
    32701 ctagttttgt tcttagtatc atgcagttga tcgggattgt ggctggggtg agtttttctt
    32761 tcttgatttc tatctttaaa gagaaactgc tcctctggtc tggtattacc tttggcttgg
    32821 ggcaaattgt gattgccttg tcttcatcct tgtgggtggt agtagcagga agtgttctgg
    32881 ctggatttgc ctatagtgta gtcttgacga cggtctttca acttgtctct gaacgaattc
    32941 cagctaaact cctcaatcaa gcaacttcat ttgctgtatt aggctgtagt ttcggagcct
    33001 ttacgacccc atttgttcta ggtgcaattg gcttactaac tcacaatggg atgttggtct
    33061 ttagtatctt aggaggttgg ttgattgtaa tctctatctt tgtcatgtac ctacttcaga
    33121 agagagctta ggattgattc ctaagttttt cttttgatac tcaatgaaaa tcaaagagca
    33181 aactatagtt gattgagttt ggaatagtac gctgtagctg ctaaaatatt tctagaaatt
    33241 aatttgactt tcctaataga gttattcata tcttatttca atctactata gaaagctagc
    33301 cgcaggttgc tcaaaatagt gttttgaggt tgcagatgga aactgacgtg gtttgaagag
    33361 attttcgaag agtatgagat ttttataccc atacaatatt tgttttaggt ccttgtttac
    33421 agactaattt cttgataaaa tagaggttat gacgagatat aaagcaacta tttcctatga
    33481 tggttatgcc tttgctggct ttcagcgcca gtctcatgcg cgtagcgttc aggaagaaat
    33541 tgaaaaaacc ttgaccagat taaataaagg gcaagccatt actgttcacg gtgctggtag
    33601 gacagatagt ggggttcatg ccctgggaca ggtcattcat tttgacctgc cttatcagat
    33661 tgatgaggag aaactccgtt ttgccttgga tacccagtct cctgaagata ttgatgtgat
    33721 ttcgattgag cttgtggcag atgattttca ttgccgttat gccaagcata gcaagaccta
    33781 tgagtttatt gtggatagag gacgtcccaa aaatccgatg cgccgtcact atgccaccca
    33841 ctttccctac ccactcgatg tggaacgaat gcagattgca atcaaaaagc tagagggaac
    33901 ccatgatttt accggtttta cagcctctgg gactagtgta gaggataagg ttcgcaccat
    33961 cacagaagct agtttaatag tcgatgagac aggacaattt ttgaccttta ccttttccgg
    34021 aaatggtttc ttgtataaac agattcgcaa tatggtgggg actcttctca aaatcggtaa
    34081 caaccgcatg ccagtagagc agattgatct catcttggag aagaaggaca ggcaacttgc
    34141 aggtcccact gcagcaccaa atggtttgta tttaaaggag attcgttatg aagaataatc
    34201 gtattttagc actttctgga aatgatattt ttagtggtgg tggactgtca gctgatttgg
    34261 ctacctatac cttgaacggc ttgcatgggt ttgtagcagt gacttgtttg acagccttga
    34321 cagaaaaagg atttgaagtc tttccaactg atgataccat ttttcaacat gaattagata
    34381 gcttgcgtga tgtggaattt gggggaatta agattggtct tctccctact gtcagtgtgg
    34441 ctgagaaggc cttggacttt atcaaacaac gcccaggagt acctgtggtg ttggatcctg
    34501 tcttggtctg caaggaaacg catgatgtag ctgtcagtga gctctgccaa gagttgattc
    34561 gctttttccc ttatgtcagt gtgattacgc ctaatctccc agaagcagaa ttattatccg
    34621 gtcaggaaat taaaaccttg gaagacatga aaactgcagc gcagaaattg catgatttag
    34681 gagcgccagc agtcattatc aagggaggca atcgtcttag tcaggacaag gctgtggatg
    34741 tcttttatga tggacagacc tttactatcc tagaaaatcc agttatccaa ggccaaaatg
    34801 ctggtgcagg ttgtaccttt gcctctagca ttgccagtca cttgattaaa ggtgataaac
    34861 ttttgccagc agtagaaagc tctaaggctt tcgtttatcg tgctattgca caagcagatc
    34921 agtatggagt aagacaatat gaagcaaaca aaaacaacta aaatcgccct tgtatcccta
    34981 ttaaccgccc tttctgtggt tctaggttat ttcttaaaaa tcccaacacc tacaggaatt
    35041 ctaactcttt tagatgctgg tgtcttcttt gcggcctttt actttggtag tcgtgaagga
    35101 gcggtagtcg gaggactagc aagtttcttg attgacctct tatcaggcta ccctcagtgg
    35161 atgttcttta gcttggtcaa ccatggcttg cagggatttt tcgcaggatt taaaggaaaa
    35221 agtcagtggt taggccttat tttagcaact attgccatgg taggaggcta cgccttgggt
    35281 tctactttga tgaatggctg ggcagcagcc ctcccagaaa ttctaccaaa tttcatgcaa
    35341 aatatggtag ggatgattgt aggatttatt cttagtcaaa gtatcaagaa gattaagtaa
    35401 tactcttcga aaatctcttc aaaccacgtc agtttccatc tgcaacctca aaacactgtt
    35461 ttgagcagcc tgcggctagc tttctagtct gattttcatt gagcataaag aggttgagta
    35521 aaaagtcttt caaataagaa aaacgcatag tatcaggtgt tgaatgaaca cctgatacta
    35581 tgcgttttat tgtgggaaga ttttcctcat tttctagtga aattgaactt tttctaacat
    35641 cttttgatag atgacttctt aatcaattat catttccatt gttgtttctt ggggtttcat
    35701 cccttgtctt tgataaaagc ggagagcacc ctcgttatca ttccagacac ttagggttag
    35761 attattgcag ccaatttctt tagcataata gcagaaaaat gacaataaaa tagcccctag
    35821 atattttgaa aggaaagcat aggggctata tagtttaatc aaaataaagc aaatctttgc
    35881 tggtgcttgt aaagaggtcg aagccatcct tggtaacaac accgcagtct tcaatacgaa
    35941 caccgacttt accagggata tagatacctg gttcaacaga gaagcacatg ccttcttcga
    36001 tgaccatgtc gtttccttcc atgatagatg ggaattcatg gacatccata ccgataccat
    36061 gcccgagacg gtggttgaag tactcaccat aaccagcttt ttcgatgacc tcacgggcag
    36121 cgcggtccac ttcatgagca gtcacacctg gcttgataaa gtcaagagca gcttgttggg
    36181 cttcaagagt caagttgtaa atatctttct tgaattggtc tggtttgccg acagcgactg
    36241 tacgagtcat atctgacgca tagccattga ccagaacacc caggtcaaag aggagaagag
    36301 catcattttc aaccttatta gctgctggaa tgccgtgtgg attcgcagca ttatcaccag
    36361 tcaagaccat ggtatcaaag ctcatttcgt aaccttcacg tttcatggca aagtcaattt
    36421 gtgcgatgat atctgtctca gtcttatcaa gagaaatatt gtcaaaacca acatgaacag
    36481 ccttgtcagc ataaagacct gcaaccatca ttttttgcac ttcatcagct gatttgatga
    36541 ggcgcatgcg ttggatacga ggagtgaggt tgtcaaactc agcagtctca aaaactgttt
    36601 tcaaaccatg gtatttggtc aagatgagat tgtcaaactc aacagcgaca cgtttgaagt
    36661 caagttgtgg aagagcatgt ttgatttttt gccatggatt ttcagaatcg acatagccca
    36721 ctactgggaa ggaaacggtg ctacttgcac gttctacttc aagagctggg acaaagagga
    36781 gaggttcctg atctgctagg acaaagagga acatttggcg ttcatgggga tcactgtaaa
    36841 aaccagtgag gtaattgatt gtgacggggt cagatacgac agcgacgtct agtttttctg
    36901 attcaagata tgttaggatt tgttgtaatt tagacatatg ctaccttctt tctatccctc
    36961 tattttggca aaaaatgaga gaaaatgcaa gggagaaggg taaaagaaaa gacaaaaaac
    37021 tccgacaaga taacggagtt ctttgatatc attttccttt gtttaagaaa agtcagcctt
    37081 gcttttgacg tcactgtact cttcagcgat ttcttggctg aggatatcct cataggcagg
    37141 gagttggatg tcctgaccat atttcttctt gagggcagta gtaactaggc gataagccag
    37201 attggcatta cgagagagga taggcccgtg gaagtaggaa ccaaagacat tcttataatg
    37261 aaccccttca ccgacctttt cttcgttgtt tccatttcca tagacaacct gtcccagcgg
    37321 tttttggtca tcagagagga aggtacggcc ttggtgattt tcaaatccat agtaggtttc
    37381 atcgaaatct tcattgtgaa tcttgatgtc accgataaaa cggttattgg tctggttgag
    37441 cgtgtagtgt cccatgaccc ctagcccttc gatacgtttt cctgaagctt caacataata
    37501 ttgacccaat agttggaaac caccgcagat agccagaact acaccgtcgt tttggatgta
    37561 gttgtcaatg ctctcttttt tagcaggtag gtcgtctgca atgatacttt gttcaaagtc
    37621 ttgaccacca ccgaaaaagg cgatgtcgta gtgattttca tcaaagtcat catggagaga
    37681 aacgatgtca acggtcacat gggctcccag tttttcagcc acatacttga gcatgaggat
    37741 gtttccattg tccccgtagg tattcatgag atttccgtag aggtgggcaa tgttgagctg
    37801 ataggggtaa ttgccatctt ttgaggaaag tgaagtataa accattagtt catctccttt
    37861 ctaacaatct gacgactagc cagcagttca cgaaattcca gcatggcagt ataagttgcc
    37921 agaatatagg catgcttgca gtcttgattc tcaatggtct tgagaacttg ctccagatta
    37981 ctcgtttcag tgattttctc agctggatag ccagtcactc ggaggcgacg agcgatttca
    38041 gaatgacgaa caccgccagc gttgatttca ggaatgtcca tgtcagtgat ttgttcaaag
    38101 tctgcatccc agatccagct agtgtcaatt ccatctgcat agttggcatt aaggaggaca
    38161 gataggctaa atggataagg tgctagtttg atcatttcga tagcttgggt tgcaccgact
    38221 ggatttttaa tcaagacaag ggtacattcc ttgtcaccga tatgaaaggt ttcttggcgt
    38281 ccaaagacag cacggctctt gtcaaatccc tgtttgatga gttgcgaatc ggcacctagg
    38341 aaacgggcga tggccacagc agctagggcg ttatagatat tatagagccc gccgatttgg
    38401 ataccgtatt cttggacgtc tatgacaaag cgagagcgat tgttggtcaa ctcaaccagt
    38461 tttgtcaaac gatagtcgag atcaggacgt ttacatccac aaccttcaca gatataggta
    38521 cccaagtttg cataggtatt atgctcatat ttgaggatgc cttggcagtc aggacagaga
    38581 atcccttcgg tattgtagtg agccagttgg gctggtcctt tttccaagtc aaaaccaaaa
    38641 tactctatag ggtttggaat agttggcttg tagaaaagtg gactgtctcc gttaaggaga
    38701 acagtagcag ttggaacttt ccgaatggca tccaatatca tgttataggt agtatagatt
    38761 tcaccgaaac ggtccatctg gtcacggaag atattagtaa tgacaaaaag actaggctgg
    38821 atatagtcac agatacgaga tagactggct tcgtcaattt cgaggacggc aatatttttt
    38881 ccagttttag aagatttggc tgttaggaag gttgttgcaa tccctgtaat catgttggca
    38941 ccgcttggat tggttagaac ttgaccatag acctctttta aaatgccgac agtgagggca
    39001 gttgtcaggg tttttccatt tgttccagtg acaacgacaa tctcgtagtt cttagctagg
    39061 ttttgtaaaa tatctttatc aaattgaagg gcgactttac ctgggagcgt acttccacgg
    39121 ccaagacggc ttaaaatgaa gtgggaagaa cgcccagcaa gaaggcccaa agtagttttt
    39181 aagttcatgt ttctattata tcataaaaga ggagaaagac agatttgaga ttccgcagaa
    39241 gtaaaaacag cccaaagagg gctgttcgat acgattaaat ctcaacttaa atcgcaaaca
    39301 agtcattcaa gttctcagcc aaggttgggt gagtgaagat ttgttttgtg aagtaagtgt
    39361 aaggaatctt gttgtccata gcaacagtga tgatgttgat gatttcttga gaaccttctg
    39421 agaagatgct tgctccaaga atttcttttg tttcagtatt gacaacagct ttgaaggcac
    39481 cgcgaaggtc tccatttacg tgaccacgag gcatggctgc aacggggatt tccttaacag
    39541 cgtatggaag tttcaaatca gctgcttggc tttcagtcaa accaacttgt gaaagtgcag
    39601 gtgtgatgaa catagtattt ggcacattga gacggtcttc aagtgtgtag ctgccatctc
    39661 cagcaaggta gctgtaaaca acacggaagt catcaagtga aatgtaagta aattgaaggc
    39721 caccgttgat atctccaact gcaaagacac caggaacgtt tgtttgacag tgtttgtcta
    39781 ctttaatagc accacgttca gttagttcaa tatctgtatt ttcaagttga agtggttcta
    39841 catttggttt gcgtccagtt gcgtagagaa gggcgtcgaa acggtaagtt tcgtcttcag
    39901 ttacgacaag cacttggtca ccatcgtttt tgatttcagt agtatggata ttttgaagca
    39961 attcaatgcc atcttcttcc atgtattgtt tagcaagagc tgcgatggaa ggttctgcac
    40021 gaggaaggaa agtatccaag gcatctagga ctgtgacctt gcttccaagt ttgttgtaaa
    40081 ggccggcaaa ttcaagaccg atatttccgc caccaaggat tccaagtttt tcaggtaatt
    40141 tgtccaagtt ttggatacct gttgagtcaa agatgttttt gcttgtagca agtccaggga
    40201 ttggcaagac gtttgaaaca gcaccagtgt tgatgacgat tgtttcagca gtcagttctt
    40261 tcttttcatc accagcttgg atttcgatga ctttatttga aaggaagtga gcttccgcat
    40321 caaagatatc tacgcctgta ccagcaacag tcgcatagtt tttaccgttg aggcgaccag
    40381 tgatcgtgtt tttagtagca atcacttctt caaaagacaa gtccttttca gcagcaacta
    40441 gcaaggtttt agttgggata caaccaatgt tgatacaagt tccaccgtac atagccttgc
    40501 tgcgttcaac gagggcaact tttttgccag ctgaagccaa tttacctgct agtgttttac
    40561 cagctttacc aaatccaata acaattaaat cgtatgttaa catttatctt cctcctattc
    40621 gaatttcatt ctagcacaag aaaaaaactt ttgcacaatt ctgctacgga aaagacagaa
    40681 aaagataaaa acgctttcca gacaaaaaac tagtaatttt ttgacaagct tgataaacta
    40741 aaatcctatc tatttttata taagatagaa taaatgttcg aaaataggag gttttatgaa
    40801 atcgaatcgt tatattattg cctttgctgg ggtcatttta cacttaatgc tgggttcgac
    40861 ttatgcttgg agtgtttatc gtaaccctat tattgaaaaa acgggatggg atcaggcttc
    40921 tgttgccttt gcctttagtc tagcaatctt ttgtttgggc ttatcggctg catttatggg
    40981 gcgtttggta gaaaaatttg gtccgagagt catggggagt ctatctgctt ttctatacgc
    41041 aggtggaaat atcttaacag gatttgcaat agaccgtcag gagctgtggt tgttgtatct
    41101 cgcttatggc attttaggtg ggcttggttt gggagcaggc tatattaccc ctgtgtcgac
    41161 gattataaaa tggtttcctg ataaacgtgg tctcgcaaca ggtttagcga ttatggggtt
    41221 tggttttgct tctttattga ctagtcccat agcgcaacac ctcatcgcag gggtagggct
    41281 tgtagaaact ttttatattt taggagcaag ttactttatt atcatgctcc tagcttcaca
    41341 attcattaag cgtccaaatg agcaagagct tgcaatttta tcttcttcag ggaaagaaaa
    41401 aacagcctct ttgacgcaag gaatggctgc aaatcaggct ctaaaaagca atcggtttta
    41461 tatactttgg attattttct ttatcaacat agcttgtggt ttaggcttaa tttcagcggc
    41521 atcgccaatg gcacaggaga tggctggctt gtctacaagt catgcagcag taatggtggg
    41581 cgttttaggg attttcaatg gatttggtcg cttgctctgg gcgagcttat ctgactatat
    41641 cggtcgccct ctaaccttta gtatattact gcttgttaat cttttctttt ctctctcact
    41701 ttggctcttt acagattccg ttttatttgt agtcgctatg tctattttga tgacttgcta
    41761 tggagctggt ttttcattga tcccagctta tctcagtgat atttttggaa ccaaggaatt
    41821 ggccgctctg catggatata ttttaacagc ttgggcaatg gctggtttag cgggacctat
    41881 tttattagca gagacttata aaatggctca ttcgtacaca caaaccttgt tcgtttttct
    41941 cattttatac agtatcgcct tggctttgtc ttattatcta ggtcgttcaa tcaaaaaaga
    42001 aagtcaaaaa ccgcttacat gattttctgt agaaatgggg ctatcttttg ctctggtctt
    42061 gtgttatact agataggttg caaagaaaac agtacttttc ttttgtggaa aagaagcaac
    42121 acgattttat atctagttat atgaaaatac gtcataaaaa gaaaagtata aactaagccc
    42181 tatagggtgg cttcgcacca cctttagaaa gaagaataac gtgaaattta atgaattaaa
    42241 cttgtctgct gatttgctgg cagaaattga aaaagctggt tttgtagaag ctagtcctat
    42301 ccaagaacaa actattcctt tggcccttga aggcaaggat gttatcggtc aagctcagac
    42361 tggtacagga aaaactgcag cctttggctt gcctaccctt gaaaaaatcc gtacagaaga
    42421 agcgactatc caagccttgg tcatcgctcc aactcgtgaa ctagctgtcc aaagtcaaga
    42481 agaactcttc cgctttggtc gtagtaaggg agtcaaagtc cgttcagtat atggcggatc
    42541 aagcattgaa aaacaaatta aggctcttaa atctggtgcc catatcgtgg tgggaactcc
    42601 aggtcgcctc ttggacttga ttaaacgcaa ggccttgaaa ttacaaaaca ttgaaaccct
    42661 tatccttgac gaagcggatg aaatgcttaa catgggcttc cttgaagaca tcgaagccat
    42721 tatttcccgt gtacctgaga accgtcaaac tttgcttttc tcagcaacta tgccagatgc
    42781 catcaaacgt atcggtgttc agtttatgaa agcccctgaa catgtcaaga ttgcagctaa
    42841 ggaattgaca acagaattgg ttgaccagta ctatatccgt gttaaggaac aagaaaaatt
    42901 tgacaccatg actcgtctca tggatgtggc acaaccagaa ctcgctattg tatttggtcg
    42961 taccaaacgc cgtgtggatg aattgactcg tggtttgaaa attcgtggct tccgtgcaga
    43021 aggaattcat ggcgacctag accaaaacaa acgtcttcgt gtccttcgtg actttaaaaa
    43081 tggcaatctt gatgttttgg ttgcgacaga cgttgcagcg cgtggtttgg atatttcagg
    43141 tgtgacccat gtctacaact acgatattcc acaagatcct gagagttatg ttcaccgtat
    43201 cggtcgtaca ggtcgtgctg gtaagtcagg tcaatctatt acttttgttg ctccaaacga
    43261 aatgggttac cttcaaatca ttgaaaactt gactaagaaa cgcatgaaag gtctcaaacc
    43321 tgcaagtgta gaagaatcct tccaatcaaa aaaacaggta gctctcaaga aaatcgaacg
    43381 tgattttgca gatgaaacca ttcgtgccaa ctttgagaaa tttggtaagg atgctcgcaa
    43441 attggctgct gagtttactc cagaagaatt ggcaatgtat atcttgagtc tgacagtcca
    43501 agacccagat agccttccag aagtggagat tgcacgtgaa aaaccactac cgtttaaacc
    43561 atcaggtaat ggtttcggtg gtaaagctaa gggaggtcgt ggaggccgtc gtggggacga
    43621 ccgtcgagag cgtgatcgcc gtggcaatgg tcgccgtgat gagttcaaaa aaggaagtcg
    43681 tggcaacgat cgttttgata aggaaaaacg ttaccgtaag gataataaaa aaccacgcaa
    43741 tactttaagc gaaaagcaaa caggctttgt tattcgtaac aaaggcgata aataagaaaa
    43801 agcggttcaa aatgaatcgc ttttttatat aaggagaccg aatgtgaaaa gacaaataaa
    43861 attattatat tatcttttat aatgttatga tacacaaaaa taggcaactt gtcaatagaa
    43921 aaggaacaat ttaattaaaa atattctggt ttttaattaa attgtaagtc aatgcaagtc
    43981 aataaggaat tttcagataa ttattgaaaa agagacgttt ttatgttata atgatagcga
    44041 ttaaattcga ggtgaaaaat ggcacattta ttagaaaaaa ctagaaaaat tacatcaatt
    44101 ttgaaacgct cagaggagca gttgcaggat gagctccctt acaacgctat tacgcgtcag
    44161 ttagcggata ttattcattg caatgcctgc attatcaata gtaagggacg tctgcttggc
    44221 tattttatgc gttataaaac aaatacagat cgcgtagagc aattcttcca aactaagatt
    44281 ttcccagatg actacgttca aggggctaat atgatttacg aaacagaagc aaacttgcct
    44341 gttgagcatg atatgagtat tttccctgtt gagagtagag atgattttcc agatggcttg
    44401 acgactattg caccgattca tgtatcgggg attcgccttg gttctttgat tatttggcgt
    44461 aatgataaaa aattcgaaga tgaggacttg gttcttgttg agattgccag taccgttgtt
    44521 gggattcagc ttcttaactt ccaacgtgaa gaagatgaga aaaatattcg tcgtcgtact
    44581 gctgtcacca tggcggtcaa taccctttct tactccgaac tccgtgctgt ttcagcaatt
    44641 ttaggggaat taaatggaaa tgaagggcag ttgactgcgt cagtgattgc agatcgtatc
    44701 ggaatcactc gctctgtgat tgtcaatgct cttcgtaaac ttgagtctgc ggggattatt
    44761 gaaagtcgct cacttggaat gaagggaacc tatcttaagg tcttgatttc agatattttt
    44821 gaagaagtga agaaaagaga ttactaatga caaaggcttt aatttcgatt gattatacag
    44881 aagattttgt tgctgatagt gggaagctga cagcaggtgc tccagctcag gcgatttcgg
    44941 atgccattag caaggtaact cgattagctt ttgaacgagg agattatatc ttctttacta
    45001 ttgatgctca tgaagaaaac gattttttcc atccggaaag taagttattt cctcctcata
    45061 atctgattgg gacgagtgga cggaatttat atggagattt ggggatcttt tatcaagagc
    45121 atggttcaga cagtcgtgtc ttttggatgg ataaacgcca ttactcagct ttttcaggga
    45181 ctgacctaga tattcgtttg agagagcgta gagtgtctac cgttatctta acaggtgtct
    45241 tgacggatat ctgtgtccta catacagcta tagatgccta taatctagga tatgacatcg
    45301 agattgttaa accagctgtt gcttccatct ggcctgaaaa tcatcaattt gccctaggtc
    45361 atttcaaaaa tacactcgga gctaagttag tagatgaaaa tctaaatgaa ctttctgagt
    45421 aacctggttg gtgaaaatga aaaaatttga aaaaaagtgt tgacaagcat cccaaaagtt
    45481 gatatactag taaagtaatc gatgcgggga tggcggaatt ggcagacgcg caggactaag
    45541 gatcctgtga ccgctttagg tcgtgagggt tcaagtccct ctctccgcat aggaaaagag
    45601 ttagctaaag ctagctcttt tgtatttctc taaaaaatta gaatccagtc aaatgtagtg
    45661 gagaatcagc gaaaatatgg gaaaactgat ccttgtttct gcttttgtca tagaagaata
    45721 ataaagatta tcttcttcaa attctccgat attctctaaa gttttgtgca agttgcacag
    45781 aactttttta tttttttggt catcttgcca tagaaatata aagcgttttc atatataata
    45841 taattatcaa aagacaaaag gagttcacct catggtagaa ttgaatctta aaaatattta
    45901 caaaaaatat ccaaacagcg aacactattc agttgaagat ttcaacttga acataaaaga
    45961 taaagaattt atcgttttcg taggaccttc aggatgtggt aaatcaacta cactccgtat
    46021 gattgctggt cttgaagaca ttacagaagg tactgcatct atcgatggcg tggttgtcaa
    46081 cgacgtagct ccaaaagacc gtgatatcgc catggtattc caaaactacg ctctttaccc
    46141 acacatgact gtttatgaca acatggcttt cggcttgaaa ttgcgtaaat acagcaaaga
    46201 agacattaac aaacgtgttc aagaagcagc tgaaatactt ggattgaaag aattcttgga
    46261 acgtaaacca gctgaccttt caggtggtca acgtcaacgt gttgccatgg ggcgtgcgat
    46321 tgtccgtgat gcgaaagtat tcttgatgga cgaacctttg tcaaacttgg atgccaaact
    46381 tcgtgtatca atgcgtgctg aaatcgctaa aattcaccgt cgtatcggag ctacaactat
    46441 ctatgtaact cacgaccaaa cagaagcgat gacacttgca gaccgtatcg ttattatgtc
    46501 agctactaag aaccctgctg gtacaggtac tatcggacgt gtagaacaaa tcggtactcc
    46561 tcaagaagtt tacaaaaatc cagttaacaa attcgttgca ggattcatcg gaagcccagc
    46621 tatgaacttc atcaccgtga aattggttgg tagcgaaatt gtttctgacg gtttccgttt
    46681 gaaagtgcca gaaggagcat tgaaagttct tcgtgaaaaa ggctacgaag gaaaagaatt
    46741 gatctttggt atccgtccag aagacgtgaa tgcagaacct gctttccttg aaacattccc
    46801 agactgtgtt gtaaaagcga ctatctctgt atcagaactg cttggttcag aatctcacct
    46861 ttactgccaa gttggtaaag acgagtttgt tgcaaaagtt gatgctcgtg actacttgca
    46921 aacaggtgca acagttgagc ttggatttga cttgaacaaa gcacacttct tcgatgtaga
    46981 aactgaaaaa acaatctact aaaataaata aaattcaaag cactacaaga aaagatatct
    47041 ctttatcaat tgtagtggag agatatcagt taatctaggg agagaaacaa aatgcttctc
    47101 tcctttttgc tagggaagtc atattatgca tctatattgt gatgctcttt atactcttcg
    47161 aaaatctctt caaaccacgt caacgtcgcc ttgccgtacg tatgattact gatttcgtca
    47221 gttttatctg caacctcaaa gatgtacttt gagcagctta cggctagttt cctagtttgc
    47281 tctttgattt ccattgagta ttattgtgag taccatctac aagtgaagct atatgcgtaa
    47341 actacgtgag caattgaatt cgaactagag aggtaataat aaatttatgc tatagttatg
    47401 gtgacttgta tgcttttgat tctagtttat caaataatag attagaattg tcagataata
    47461 tcattttgtg ttataatgaa gaaaaaacag aggtgttcaa atgtcagaag caggtcataa
    47521 gtttttagca aaattgggga aaaaacgctt acgtccaggt ggaaagcgtg ccacagattg
    47581 gttaattgca gaaggaggat tttcaaaaga aaagagaata ctagaggttg cgtgtaatag
    47641 gggaactaca gcaattgagt tggcacagcg ttttggttgc aagataactg ctgttgatat
    47701 ggatgctcaa gctttagaag tggctaaaaa atctgctgga acggcaggtg ttgctcattt
    47761 aatcagtttt gaaagagcaa atgcaatgaa acttccttat caagatgcta gttttgatat
    47821 tgttataaat gaagctatgc tgactatgca agccgatcaa gctaagaaaa aatgtgtaat
    47881 ggaatatcta agggtattaa aacctggagg tcttctcttg acacatgatg tgcttcttaa
    47941 ggaagctaaa gagtctatca gacaggaatt atcacaagca attcatgtaa atgtaggtcc
    48001 tttaactcaa gatggttggg aacaggtgat gatagaatca ggttattgtg atgtgaaagc
    48061 attgactggt gaaatgacat taatgaaatt atcgggtatg atttatgacg aaggtttgct
    48121 aggaactttg aaaatttgtg taaatgcttg taaaaaggag aatagaaagc agtttttaac
    48181 tatgtataaa atgtttgcta agaataaaca gaaattgggc tttattgcga tggctagtta
    48241 taaatcgtca aaacgttaga taattattga agttaacttt tccttttttc tttctt
//
LOCUS       DAVLRT010000006        46590 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLRT010000006 DAVLRT010000000
VERSION     DAVLRT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.006"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(613..1026)
                     /gene="ndk"
                     /locus_tag="VXM85_000319"
     CDS             complement(613..1026)
                     /gene="ndk"
                     /locus_tag="VXM85_000319"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1344951.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(1137..4814)
                     /gene="rpoC"
                     /locus_tag="VXM85_000320"
     CDS             complement(1137..4814)
                     /gene="rpoC"
                     /locus_tag="VXM85_000320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1344952.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(4847..8458)
                     /gene="rpoB"
                     /locus_tag="VXM85_000321"
     CDS             complement(4847..8458)
                     /gene="rpoB"
                     /locus_tag="VXM85_000321"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1344953.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            8874..8944
                     /locus_tag="VXM85_000322"
     tRNA            8874..8944
                     /locus_tag="VXM85_000322"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8906..8908,aa:Cys,seq:gca)
     gene            complement(9142..10473)
                     /locus_tag="VXM85_000323"
     CDS             complement(9142..10473)
                     /locus_tag="VXM85_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1344954.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(10764..11588)
                     /gene="endA"
                     /locus_tag="VXM85_000324"
     CDS             complement(10764..11588)
                     /gene="endA"
                     /locus_tag="VXM85_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1344955.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11627..11815)
                     /locus_tag="VXM85_000325"
     CDS             complement(11627..11815)
                     /locus_tag="VXM85_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1344956.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11808..13091)
                     /gene="murA"
                     /locus_tag="VXM85_000326"
     CDS             complement(11808..13091)
                     /gene="murA"
                     /locus_tag="VXM85_000326"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1344957.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(13115..13345)
                     /locus_tag="VXM85_000327"
     CDS             complement(13115..13345)
                     /locus_tag="VXM85_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1344958.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(13403..14440)
                     /locus_tag="VXM85_000328"
     CDS             complement(13403..14440)
                     /locus_tag="VXM85_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1344959.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     INDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(14424..14912)
                     /gene="coaD"
                     /locus_tag="VXM85_000329"
     CDS             complement(14424..14912)
                     /gene="coaD"
                     /locus_tag="VXM85_000329"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1344960.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPIENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(14902..15441)
                     /gene="rsmD"
                     /locus_tag="VXM85_000330"
     CDS             complement(14902..15441)
                     /gene="rsmD"
                     /locus_tag="VXM85_000330"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1344961.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQITKEIGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYTKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(15506..16498)
                     /locus_tag="VXM85_000331"
     CDS             complement(15506..16498)
                     /locus_tag="VXM85_000331"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1344962.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(16609..17292)
                     /locus_tag="VXM85_000332"
     CDS             complement(16609..17292)
                     /locus_tag="VXM85_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1344963.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(17348..18088)
                     /locus_tag="VXM85_000333"
     CDS             complement(17348..18088)
                     /locus_tag="VXM85_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1344964.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQREEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18122..18400
                     /locus_tag="VXM85_000334"
     CDS             18122..18400
                     /locus_tag="VXM85_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1344965.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            18478..19404
                     /gene="yidC"
                     /locus_tag="VXM85_000335"
     CDS             18478..19404
                     /gene="yidC"
                     /locus_tag="VXM85_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1344966.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(19479..20273)
                     /gene="pflA"
                     /locus_tag="VXM85_000336"
     CDS             complement(19479..20273)
                     /gene="pflA"
                     /locus_tag="VXM85_000336"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1344967.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(20399..21649)
                     /locus_tag="VXM85_000337"
     CDS             complement(20399..21649)
                     /locus_tag="VXM85_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1344968.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21716..22543)
                     /gene="purR"
                     /locus_tag="VXM85_000338"
     CDS             complement(21716..22543)
                     /gene="purR"
                     /locus_tag="VXM85_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1344969.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22810..23751)
                     /locus_tag="VXM85_000339"
     CDS             complement(22810..23751)
                     /locus_tag="VXM85_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1344970.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(23741..24997)
                     /locus_tag="VXM85_000340"
     CDS             complement(23741..24997)
                     /locus_tag="VXM85_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1344971.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(24999..25661)
                     /locus_tag="VXM85_000341"
     CDS             complement(24999..25661)
                     /locus_tag="VXM85_000341"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1344972.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAEV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25624..26280)
                     /locus_tag="VXM85_000342"
     CDS             complement(25624..26280)
                     /locus_tag="VXM85_000342"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1344973.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(26291..27169)
                     /gene="rsgA"
                     /locus_tag="VXM85_000343"
     CDS             complement(26291..27169)
                     /gene="rsgA"
                     /locus_tag="VXM85_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1344974.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(27171..28043)
                     /gene="rsmA"
                     /locus_tag="VXM85_000344"
     CDS             complement(27171..28043)
                     /gene="rsmA"
                     /locus_tag="VXM85_000344"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1344975.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILVDTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(28429..28575)
                     /locus_tag="VXM85_000345"
     CDS             complement(28429..28575)
                     /locus_tag="VXM85_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344976.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDKNMTNHLLLVQILVHGYLLDFA
                     SIEG"
     gene            complement(28568..29209)
                     /locus_tag="VXM85_000346"
     CDS             complement(28568..29209)
                     /locus_tag="VXM85_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344977.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(29211..31241)
                     /locus_tag="VXM85_000347"
     CDS             complement(29211..31241)
                     /locus_tag="VXM85_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1344978.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(31318..31614)
                     /locus_tag="VXM85_000348"
     CDS             complement(31318..31614)
                     /locus_tag="VXM85_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1344979.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(31934..32833)
                     /locus_tag="VXM85_000349"
     CDS             complement(31934..32833)
                     /locus_tag="VXM85_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1344980.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(32905..33465)
                     /gene="rnmV"
                     /locus_tag="VXM85_000350"
     CDS             complement(32905..33465)
                     /gene="rnmV"
                     /locus_tag="VXM85_000350"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1344981.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(33465..34238)
                     /locus_tag="VXM85_000351"
     CDS             complement(33465..34238)
                     /locus_tag="VXM85_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1344982.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPMIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIRLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(34543..35436)
                     /locus_tag="VXM85_000352"
     CDS             complement(34543..35436)
                     /locus_tag="VXM85_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1344983.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEVTPEY
                     KLETTPGGDKGGNIGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRLVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(35720..35854)
                     /gene="rpmH"
                     /locus_tag="VXM85_000353"
     CDS             complement(35720..35854)
                     /gene="rpmH"
                     /locus_tag="VXM85_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1344984.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(35999..37213)
                     /locus_tag="VXM85_000354"
     CDS             complement(35999..37213)
                     /locus_tag="VXM85_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1344985.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(37369..37662)
                     /locus_tag="VXM85_000355"
     CDS             complement(37369..37662)
                     /locus_tag="VXM85_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344986.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            37811..38263
                     /locus_tag="VXM85_000356"
     CDS             37811..38263
                     /locus_tag="VXM85_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1344987.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(38298..39686)
                     /locus_tag="VXM85_000357"
     CDS             complement(38298..39686)
                     /locus_tag="VXM85_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1344988.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELHRQL
                     KAYERHKERNKS"
     gene            39752..40714
                     /locus_tag="VXM85_000358"
     CDS             39752..40714
                     /locus_tag="VXM85_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1344989.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HTLDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLTMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(40711..40902)
                     /locus_tag="VXM85_000359"
     CDS             complement(40711..40902)
                     /locus_tag="VXM85_000359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344990.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHVFEVVDRTDEVS
                     NHTYGKATLTWFAEIFEEY"
     gene            complement(40966..41976)
                     /gene="ccpA"
                     /locus_tag="VXM85_000360"
     CDS             complement(40966..41976)
                     /gene="ccpA"
                     /locus_tag="VXM85_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1344991.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(42401..43000)
                     /locus_tag="VXM85_000361"
     CDS             complement(42401..43000)
                     /locus_tag="VXM85_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1344992.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVAVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(43002..44099)
                     /locus_tag="VXM85_000362"
     CDS             complement(43002..44099)
                     /locus_tag="VXM85_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1344993.1"
                     /translation="MLERLKSIHYMFWTSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMCEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     DSMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(44099..44836)
                     /locus_tag="VXM85_000363"
     CDS             complement(44099..44836)
                     /locus_tag="VXM85_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1344994.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGDINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(44838..45722)
                     /locus_tag="VXM85_000364"
     CDS             complement(44838..45722)
                     /locus_tag="VXM85_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1344995.1"
                     /translation="MDMIKVQGLHKNIKGKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELVAFFQSIY
                     PNPLSNQEIDQLLQFDKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVQDLKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(45709..45903)
                     /locus_tag="VXM85_000365"
     CDS             complement(45709..45903)
                     /locus_tag="VXM85_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344996.1"
                     /translation="MKKAFLYLFIGLSLVVWLVEMFTGWFDQALLRQFIRGAWGLGFM
                     IFIAFPMGMEWLKGEYHGHD"
     gene            complement(45900..46091)
                     /locus_tag="VXM85_000366"
     CDS             complement(45900..46091)
                     /locus_tag="VXM85_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344997.1"
                     /translation="MKNKMIVAMSLVAAGVMTYLMFSGLDEDFYHFPWELFAGFGMMS
                     WLIREGLKLVSDVKKEFEK"
BASE COUNT        13335 a        10802 c         8835 g        13618 t
ORIGIN      
        1 ttattccatg ttattttgag gaagatattg tgaaagtcaa agatgatgat aaagttgagt
       61 ggaatttgtt agaatttcaa aaatttagag catttttgac ttagtaatct gtgttgaagg
      121 ctcaaaacct atggtaaaaa agtaactttg aaaacgtatt gcctccaaag atttagttaa
      181 ataatgattt aacacaaaaa gaaattattg aagttctgga aagatgttgt ttcagtattg
      241 agaaaaggtg ggaaaaactt gcgattttca cagagaaagg aagaaaaagt atagaaatat
      301 agtcaattga aacaagaaca ggataaaaga accttttgtg ccatattttt ctcctttcgc
      361 tttacaattg gattgaacac ctttattgta tcgcgtttgg agtttttttg gtataacctt
      421 cgacgcgcac ccgcatagcg ggtgtttttt tgtctcgcac ctaacggagc gagacgaact
      481 aatagtcact taatcaaaaa acgcaccata tcaaaaacta aaaagtttga tatcatgcgt
      541 catgtcttaa actaattgac tatactttct attcaaatga gcttttaacc aattgattga
      601 gccaatccac tcttaaaacc aaagagcaat ttctcgctta gctgactctt ctgaatctga
      661 accatgtaca acattttgga taacctcatt ttctccagca gcttttgcaa agtcacctcg
      721 aatagtgcct ggtaaagctt cttctggacg agttgcaccc atcatggtcc gccaagtttc
      781 gattactttg ggaccagaaa tgacacccac aagaactgga cctgaagtca tgaattcacg
      841 aatcggtggg taaaaactct gaccaaccaa gtcctgatag tgctggtcaa tcaactcttc
      901 tgaaacctgt gaacgaaact ccaatttttc gattgtaaat ccacgttgtt cgatgcgctt
      961 taacacttca cccactagcc ctctttttac accatctggt ttgatgataa agaatgtttg
     1021 ttccataccc gtctcctttg tcagcttctt tcttttattt taccacattt cgtggaaaaa
     1081 tggagaaagt tttcagaaga gagaatgaga gaacccgagg gttctctcat tctctcttat
     1141 tctactgttt cttccacagt ttcaacggca gtatccacaa ctacttctgt tgtttcttca
     1201 tttccttctt cctctactgg aggattaagg tattcttctt cgttgacagc atgtggttca
     1261 aggttacggt aacgggccat accagtacca gctgggatga tcttaccgat gataacattt
     1321 tctttaagtc caaggagatg gtctttctta ccacggatag ctgcgtcagt aaggacacga
     1381 gttgtttcct ggaaggaagc cgctgacaag aaactgtttg tttcaagtga ggctttggta
     1441 attcccataa ggactgggcg acctgtcgct ggaactccac ctgcgataag gacatctttg
     1501 ttggcatctg taaagtcatt gatatccatg agggtaccca tgagaagatc tgtatcacct
     1561 ggatccatga cacggacttt acggatcatt tgacgaacca ttacctcgat gtgtttgtca
     1621 ccgatttcta ccccttggct acggtaaact ttttgtactt caccgagaag gtacgtttca
     1681 actgacaaga catcacgaac tgcaaggaga cgttttggtt ggatagaacc ttctgtcaga
     1741 gcagcaccac gcgctacttg gcccccaact tcgacacgca tacgagctgt aaatggaacg
     1801 acatattcac cttcgccagt ttcaccctta acaaagactt tcttggtacg agttgatgca
     1861 tcctcttcga tagcagtaac ttgtccttta acctctgtga taaccgcttc ccctttagga
     1921 ttgcgggctt caaagatttc ttggacacga ggaagaccct gagtgatatc ggtatttgag
     1981 gcaaccccac ctgtgtggaa ggtacgcatt gtaagctgtg taccaggttc cccgatagat
     2041 tgggcagcga ttgtaccaac tgcttcacca acttcaaccg catcaccagt cgccaagttg
     2101 ataccgtaac agtgacggca gacaccgtga cgagtgttac atgtaaatac agaacggata
     2161 gtcacttctt ccacaccagc attgacaatt tcacgcgcct tgtcttctgt aatcaattca
     2221 tttggaccaa taatcactgc accagtttct ggatgtttaa cagttttctt agtgtaacga
     2281 ccgttgagac gctcttcgag agactcgatc atctcttttc cttctgcgat agaacggatc
     2341 aagagaccac ggtcagttcc acagtcgtcc tcacggatga taacgtcttg ggcaacgtcg
     2401 accaaacgac gagtcaagta acctgagtcg gctgtcttaa gggccgtatc ggtcatacct
     2461 ttacgagcac cgtgagttga gaagaacatt tccaataccg acaaaccttc gcggaagttt
     2521 gaaaggattg gcaattccat gatacgtcca ttcggagcag ccatcagacc acgcataccg
     2581 gcaagctgtg agaagtttga gatgttacca cgggctccag agtccatcat cataacgatt
     2641 gggttcttag gatcttgatt agcaatcaag cgtttctcaa gtttttcacg ggcagcacgc
     2701 cattcagctg taacagcatt gtaacgctcg tcgtctgtga tcataccacg acggaattgt
     2761 ttggtgattt gttcgacacg tttgtgtgat tcttcaatga tttcagcctt gtcatcaacg
     2821 actgggatat cggcaatacc cactgtcaat cctgcaagag ttgagtggtg gtaaccgagg
     2881 ttcttcatgc ggtcaagtag ggcagaagtt tctgtcgtac ggaaacgttt gaagatttca
     2941 gcgatgatat ttccgaggtt tttcttcttg aatggagggt tgagctcaag attgctgata
     3001 gcttccttga tatctccacc aagtggcaag aagtatttag ctggaacacc ttctgtcaag
     3061 ttggcattgt ttggttcttg caagtatggt agcccctctg gcatgatatc gttgaagaga
     3121 attttaccaa ctgttgtaag caagacctta tgtctttgct cttctgtcca aggcttgttg
     3181 aggctgtctg ttgcgatacc aacacgtgag tggaggtgaa cataaccatt gcggtaagcc
     3241 ataaccgctt cgtcacggtc tttgaagacc attccttcac cttcgcgacc agcttcttcc
     3301 atggtcaagt agtagttacc caaaaccatg tcctgagatg gagtaactac cggtttccca
     3361 tctttcgggt tcaagatgtg ctcagcagct agcatgagga tacgagcttc tgcttgtgct
     3421 tcttctgaaa gtggtacgtg gatggccatt tggtccccgt caaagtcagc attgtaggct
     3481 tcacagacaa gtgggtgcaa gcgaagagcc ttaccatcaa tcaagactgg ctcgaaggct
     3541 tggataccca aacggtgaag ggtcggtgcg cggttcaaaa gcactgggtg ttctttaatc
     3601 acttcttcaa ggatatccca gatacgctca tctccgcgtt ccaccaagcg tttagctgct
     3661 ttgacgtttt gcacgatatc acgggcaacg atttcacgca tgacaaatgg tttaaagagt
     3721 tcaatcgcca tttcacgtgg aacaccacat tggtacatct taagagttgg accaacggcg
     3781 ataacggaac gtcctgagaa gtcaacacgt ttaccgagca agttttgacg gaagcgtccc
     3841 tgtttacctt taagcatgtg gctcaatgat ttcaatggac ggctacctgg tcctgtgatt
     3901 ggacgaccac gacgaccatt gtcaatcaaa gcgtcaactg cttcttgaag catacgcttc
     3961 tcattttgaa cgatgatacc tggtgcattt aactcaagca aacgagccaa acggttgtta
     4021 cggttgataa cacggcggta aaggtcattc aagtcagatg aggcaaaacg gccaccatcc
     4081 aactgcaaca ttggacgaag atctggtggg ataaccggaa ggatgttaag aatcatccat
     4141 tcaggtttgt ttccagactt gtaaaaggca tccaaaacat ccaaacgacg gatggctttg
     4201 acacgttttt gtccagtagc tgttttcaat tcttctttga gttcagcaat ttctttttca
     4261 agatctactt gcttcaaaag gtcttggatg gcttccgcac ccatcttggc aacaaatgaa
     4321 ccataaccat attcacgcaa gcgctctcgg tattcgcgct ctgtcatgat agacttgtgc
     4381 tcaagtggtg tatccttagg atcaatcacc acataagccg caaagtagat aacttcctcg
     4441 agggcacgag ggctcatatc aagggtcaag cccatacggc ttggaatccc cttgaagtac
     4501 cagatgtgag atacaggagc tttcaattcg atatgtccca tacgctcacg acgaactttc
     4561 gtacgcgtta cttcaacccc acagcggtca caaacaattc ctctgtaacg aatgcgtttg
     4621 tacttaccac aagcacattc ccagtctttt gtaggaccaa agatcacttc atcaaagagt
     4681 ccttcacgtt ctggtttcaa ggtccgataa ttgattgttt caggtttttt gacttctcca
     4741 taagaccatg aacggacttt acttggagaa gctagggtga tttgcatact tttaaaacga
     4801 tttacatcaa ccactatttc ttccctttct attctaagtg aactgcttat tcttgttcag
     4861 cagcttcttc tgttgcttcc gcttttgttg ctttctcagc ttcttcagct tcaaaggctg
     4921 ctttagcctc ttgggctgct ttttcgcggg ctttttcaag gtcatctacg tggatgacat
     4981 cttcgtccat tccttcatcc aagtcgcgaa gttccacttc ttggtcatct tcgtctagga
     5041 cacgcatgtc aagaccaaga gattgcaatt ctttgacaag aactcggaag gattctggaa
     5101 cacctggttt tggaattggt ttgccttttg taatagcttc ataggctttc aaacgtccgt
     5161 tgatatcgtc cgacttgtaa gtcaagattt cttgaaggac atttgacgca ccgtaggctt
     5221 caagagccca aacctccatc tcaccgaaac gttgtccacc aaactgagct ttacctccga
     5281 gtggttgttg ggtaacagtt gagtaaggtc cgactgaacg cgcatgcaat ttatcgtcaa
     5341 ccatgtggtg gagtttaatc atgtacatga ctccaacaga aacacggtta tcaaatggtt
     5401 cacctgtacg tccatcgtaa aggattgtct tggcatcgct atccatacct gcttctttaa
     5461 cagttgacca aagatcttca gagcttgctc catcaaagac tggtgtcgca atgtgaatac
     5521 caagagtacg agctgccata ccaaggtgaa gctccataac ctgaccgata ttcatacgtg
     5581 atggcacccc aagtgggttc aacatgatgt cgactggagt tccgtctgga aggtaaggca
     5641 tgtcttctac aggaacgata cgagagacaa cccctttgtt tccgtgacgt ccggccattt
     5701 tatctccgac cttaatctta cgtttttgag cgatgtaaac acgaaccaac atgttaacac
     5761 ctgattgcaa ctcatctcca tttacacgtg taaagatctt aacatcacga acgacaccat
     5821 cggcaccgtg tggtacacga agagaagtat cacgcacttc acgagacttg tctccaaaga
     5881 tagcgtgcaa gagacgttct tcagctgaaa gatctttctc acccttaggt gttactttac
     5941 ctacaagaat atcaccttct ttaacctcag caccaatacg gataatcccc atttcgtcaa
     6001 ggtctttgag ggcatcttca ccaacgtttg gaatttcgcg agtgatttct tcaggcccaa
     6061 gctttgtatc gcgcgtttct gattcgtatt cttcaaggtg aacagatgtg tagacatcgt
     6121 ccttcaccaa gcgttcgctc atgataacgg catcctcgaa gttgtaacct tcccaagtca
     6181 tgtaggcaac gattgggttt tgtccaagcg ccatttctcc attttccata gaaggtccgt
     6241 cagcgatgaa atcgcctttt tcaacgacat caccaacttt tacgagagtg cgttggttgt
     6301 aagcagtacc tgagtttgaa cgacggaatt tttggatgtg gtaaacatcc aatgaaccat
     6361 cttcacgacg aacttctacc ttgtcagcat ctgcgtaagt aactttacca tcatactgag
     6421 caatcacagc cgcaccagaa tcgtgggctg cttggtattc cataccagta ccaacgtaag
     6481 gtgcctgagg attaatcaat ggcacagcct gacgttgcat attggctccc atgagggcac
     6541 ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacctgtt
     6601 ttggtgaaac gtccatgtag tcaacaatat tagctggata ctcttggttg accccttggt
     6661 gacgtcccat gacaatcttc tcagcaaagg ttccatcttc attcagacga gagttagcct
     6721 gagctacagt atattcatct tcttcatcag ctgtcaacca aacaatttcg ttcgtgacaa
     6781 cacctgtttc acggtcaacc ttacggtatg gtgtttgaac aaaaccatat ttgttcaagt
     6841 gtccgtaaga tgacaagtta ttgatcaaac cgatgttagg tccttcaggt gtctcgattg
     6901 gacacatacg accatagtga gtgtagtgca cgtcacgtac ttcatatccg gcacggtcac
     6961 gagtcaaacc accaggtcct aaggctgaca aacggcgttt gtgagacaac tcagaaagcg
     7021 ggttgtgttg gtccatgaac tgtgacaact gtgatgaacc aaagaattct ttaactgcag
     7081 ctgttacagg acggatattg ataatttgtt gtggtgtcaa gacttcattg tcctgaacag
     7141 acatacgttc acggacatta cgttccatac gagaaagtcc caaacgtact tggttggcaa
     7201 gcaattcacc aaccgcacgg atacgacgat ttccaaggtg gtcgatatca tctacacggc
     7261 caagtccttc agccaagttg aggaagtagc tcatctcagc aaggatatct gcaggagtca
     7321 ccgtacgaac cttgtcatct gggttagcat taccaatgat cgttacgacg cgatctggat
     7381 cagttggagc aataaccttg aatttttgaa gaacaacagg ctcagtcaca acggctgcat
     7441 cgtttgggat gtagacaatc ttgttcaagt cgccatccaa atggctttca atgctttcaa
     7501 tcacgctacg agtcataatc gtaccagctt ctaccaagat ttctccagtt tcagggtcta
     7561 ccaatggctc tgcaatggtt tggttgagca aacgtgtttt aacattgagt tttttattga
     7621 ttttgtaacg accaactgct gccaagtcat aacgacgtgg gtcaaagaag cgagctacaa
     7681 gcaagctacg tgagctttca gccgtcttag gctcacctgg acgaaggcgt tcgtaaattt
     7741 ctttcaaggc ttcgtctgta cgagagtcca ttggattctt gtggatatct ttttcaacag
     7801 tgttgcgaac caattcgctg tctccaaaga tatcaaagat ttcatcatca cctgagaaac
     7861 caagagcacg aaccaaggtt gtaaatggaa tcttacgagt acggtcgata cgagtgtagg
     7921 tgatatcttt tgagtcgctt tcaagttcca accaagctcc acggttaggg ataacagttg
     7981 aaccatagcc caccttacca tttttgtcta ctttgtcgtt aaagtaaaca cctggtgagc
     8041 ggaccaactg agaaacgata atacgttcac caccattgat gatgaaagta cccatttctg
     8101 tcatgattgg gaaatcacca aagaaaactt cttgggtctt gatttcgcct gtttctttat
     8161 tgatcaaacg gaaggttaca aaaattggtg ctgagtagct agcatcgtgg atacgagctt
     8221 cttctagcgt gtattttggt tccttgattt catatccaac aaattccaac tccattgtgt
     8281 ctgtgaagtt tgaaattggc aatacatctt caaacacttc cttaagaccg tggtctagga
     8341 aagctttgaa tgagtcagtt tgaatttcaa tcaaatttgg taagtcaaga acttctttga
     8401 ttcttgaaaa actacgacgg gtacgatgtt tcccgtattg aacgtcatgt cctgccaaga
     8461 tgattctcct ttgtaaataa gttccaagcc ttgtcaatca ggcttttcta atcgtcatat
     8521 ggttgtaaac cccttatcac cgtgtcctct tgacgaattt tcagaatctt taagcctctg
     8581 ttacaaatgc tcataatctt gaaaaaaagc acaaaaagag cagctaaatc tgactttttc
     8641 agaagattta actgctgtga gccttgtctg gacaatattt cagacaaaac ctacgacaaa
     8701 tgattaccca tattataccc tatttagcta gatttttcaa ggggtttcag taggtttttg
     8761 gtaaattttt tcccatagaa aacttggcat cacattcgaa tcacgctatg gtacaaaaaa
     8821 ctgaaaaaac tattgactga aaatcatttt caaggtataa taataaacgt taaggcggta
     8881 tagccaagtg gtaaggcacg gctctgcaaa agcttgatcg tcggttcaaa tccgtctacc
     8941 gccttctata acttgattta tcaggtttca aatgaacaga aagcccaatt tgaagggctt
     9001 tttttatttt ccctcgaata aatacgtata actttaaaaa cttttggagc gagtttgtgg
     9061 cagagttctt tccatggcat aattcccttt tgaaatcaat tgaaaaaagc ttcgtcacgc
     9121 ctggtgacaa aagcctctag tttattctgt ttcttcttct atttcgactt ctgtgatttg
     9181 gacttttacc ttggtaacac gtccattctt cactttatca ttggttaaga taaactgttt
     9241 gttttggctg accaattcat agctgagttt ctcagtcgtt ggaatggttc ccactcctgt
     9301 caaataataa ccagcgatgg tatcaacgtc atcactttcc agctcaacat caaagtaatt
     9361 attgaaatca ttgagggtca tagttccttg aacaatgtag gtatcctcgc caatctgatg
     9421 aacatctatt gctgctttgt ccgtttcatc atcaatttca ccaacaattt cctctaagag
     9481 gtcttccaaa gtcactaaac cagccatacc accgtattca tccagcaaga ttgccatttg
     9541 tctttgggta tttcgcagtt cttttagcaa gtcatccaca aaaatagttt caggtacaaa
     9601 aagtggatct tgtaaaattc tcttccaaac aatattgtca aaaccgtcca caaagcctgc
     9661 cttaaggaga ctcttggtgt gaatgattcc aattacattg tccttatccc catcataaac
     9721 cgggatacga gaataatttt gttttaaaat actttggata atggcttgac tatcatcctg
     9781 aatatccacc ataaaggcat ccgttcgagg aaccataacc tctcgtgcca tcagttcatc
     9841 gagcgaaaag acaccttgta gcatctcaat ctcatcagca tccaatgttt cttcactatt
     9901 tgtcagcata taggcaattt catcacgggt cattttttca tccgcatcat cgaatgacat
     9961 aggagtcaaa tggctcaaga aattggtcga agcagctaaa agccaaacaa aaggactgac
    10021 tagttttccg atcccaatga taaccggcgc tgtacgaatt gccaaggcat cctttagatt
    10081 aagagcgatt ctcttaggat ataattcccc aaaaacgatg gaaatatagg tcaaaaatgc
    10141 caaggataga aaagttgcca cggcttgtgc tgtttcgcca ttcccaagcc aagaggcaat
    10201 cacacgtcct agagtatcag ttaaactcgc ccctgataag attgtaatca gggtgattcc
    10261 tacctgaatg gttgataaaa agtggttagg attttctagt accttcagca ggcggatgta
    10321 gcgtctgtct ccttcttccg ccttttgttc aactcgggca cgattaagag aaaccatggc
    10381 catttctgtg gctgagaaaa aggcatttaa taccgtcaag ataaacaata aaacaaattg
    10441 tagcaacaaa ttctgactac ttgggtcttc catagttctt ctcctaaaat agtatcttat
    10501 cctttattat atcacaaaat ttaatccaat acatattatt tttttcttag cgtaacttcc
    10561 atctctctcc caaaactgga agttagtcta agacggattt ccactgattt cacgcgtttt
    10621 tctcatacta aaagctaggt tgtgggaaac tggaagttag ttttagaagt tactattttt
    10681 tcttagtacc tattctagcc ctgcttaaac tagtaaaaag aactcctaca tccataagtg
    10741 acataggagt ttaggcttat cttttactga gttacagtta cttctccagt tcggtaatcc
    10801 agttgaagtc ccttttgaac attgggaact agaacattga attccaattc tccatccgaa
    10861 gacttggctt caatctgtga agctgaggga actaaatcct cgtttgaagc gtagtaaagg
    10921 gttacacggt aacggacacg cttgttttgg tccaaggctt tacgcacctt gctttcatag
    10981 tagttttgac cagtcgaata ctcggcttgt gcctgatttg cccaggctgt ctgaacagca
    11041 atgtttttag gattgcttgt tgaggcatca aaaccatcca aaccaccgat taaggcatag
    11101 cctaacaaat gacctctatc gactgcatgg gtataagagc cctttagatt cttgacctga
    11161 tgccaacctg gaggagtcca agaagttgaa ccattcccag tttctttacg attcttgtac
    11221 tgacgagtgg ccttagacaa gagggcatta gctacggttg gaacagtttc cttgcccact
    11281 gtctttgttt tattgtcagc gtagggctta cttgaaacct tggcatctag atttgtttta
    11341 ttaccattga cgataaaagc acctgagcca ttccactcca gactcccctt tatttgactc
    11401 ttgactgcgt ctgttaagac actctctgcc aatgcttgac taggagcttc agacgcttgt
    11461 tttttctgac taagattggt tttgggacta ttaggtgccg actgcatctg cttgatataa
    11521 tagctccctg tagacaaaag caataacact agcagtccga ttagtgtctg tcttgttttt
    11581 ttgttcatat ttctccttat ctttagaaaa aggctggttc tccagcctaa tttcctgtaa
    11641 atttatgaat caattcctgc cattttgctg gagacaggat agcccatgga tcttgaccct
    11701 tgcccaagat tccataacct accattagac cgattcctag agccagagta cctaaaatca
    11761 gtactatgat gactaaaagt aaacgattga ttacgtagct tgatttctta ttcatcttca
    11821 tcgcttgcct caatccgctg aatcttagca cctagctgcg ccaacttctc atggaaaccg
    11881 tagtaacctc tatccaagtg aaccaattta ccgaccacag tttctccctg tgctaccaaa
    11941 cctgtcaaaa tcaaggctgc actggcacga aggtcagttg aaagaacttc tgctccctgc
    12001 aaaggctgtc caccaacaat acgagctgta tcacggataa tctcagaatg caagcccatg
    12061 cggcgcatct cttctaggtg ttggaaacga ttttcgaaaa ctgtctccac catggttgat
    12121 tcgccttttg caactgtcat caaggctgta aattgagcct gcatatctgt tggaaatcct
    12181 gggtggggca aggttttcac atgaacagct tttagatttt ctagttgaga acgaacacga
    12241 attccttcgt cttcttcaat tacttcaaca cccatttcaa gtaacttggc aatcaaggga
    12301 cggttgtgct cccagacagc gtctcgaatc aagacatcac caccagtcat ggcagcagct
    12361 accataaagg ttcctgcttc gatacggtct tggactacat tgtgagtcgt accatgaagt
    12421 ttctcaacac cagtaatggt tatagtctct gtaccagcac ctttgacctt ggctcccatt
    12481 tcattaagga gaatggctaa gtcaacaatc tcaggctcac gcgcagcatt ctcaatcact
    12541 gtcaccccat cagccagagt cgctgccatc atcaagttct gcgttgcacc aacacttgga
    12601 aagtccatat agatatgagc accatgcaag cgttctgcct tggcttcgat gtaaccagct
    12661 gtctgactaa tcttaacccc catagcttcc agacctttca aatgaagatc aataggacgg
    12721 ctaccaatcg tacaaccacc tggcatggat accttggcat gacccacacg ggcaaggatt
    12781 ggccctaata caacgatgga ggcgcgcatc ttgctgacat acttgtaagg ggcttcctca
    12841 gtgatgtcgc cagtagcatc caccttgaca agatgagctt cctcatcaaa gtcaaccttg
    12901 gcattcaaac caccaactac ctgattcata ataaagacat ccgacaaaat cggaacattc
    12961 tgcaagacgg tctttccttc acttgctaga atagtcgctg ccaacaaggg taagactgca
    13021 ttttttgctc cctcgatcgt cacgcttcct accagacgat tatcgccacc ttgaaccaca
    13081 attttatcca tactcgtttc ctttactctt gatttcataa taatcctcta agcgcttctt
    13141 gccacaaatg atacaggcta ataaagaaag aactcaggat gtagcctatg acaatgctaa
    13201 aaaaggctac taataaacga acttttcttg tattctcatc tgtcactttt aaaaccttct
    13261 cccatctaac aagatccttt aaaaggtaaa aactcacata aataaagagc atatgactac
    13321 ttagagtgaa taataattga accatttgac tattatacca tcttctctgt ttgtgcgcta
    13381 gtttggaaac ttcactcaaa aattagggat tgtttttcaa gtaatcaatc gcatcttgta
    13441 gggttttaac gggcacgatt ttcatatccg tcttgattgt tttagccgct tctagggctg
    13501 tttggtagtt gtttttcgcg tccggatgcg ccttttgttc ttcttcgcta acagggttat
    13561 caggagcaaa gaaaatagca gcacctgccc tagccgaagc tacaaccttc ttatcaatac
    13621 ctccaatgtc tcccacatta ccatcgcggt caatggtacc tgtaccggca acaatacgac
    13681 cattacgaag atctgggtga gctatttgag tatagatagc tagactaaac atgagaccag
    13741 cacttggacc gccaatacca gctgttgaaa agctaattgg gacattgctg attacctctg
    13801 tacggtcaat caagccgatt ccaattccat ttttgccatt ttccaaggtg ataatctttc
    13861 cttctgcaga cttggtttgc ccatcctctt cataggtgac cttgacggaa tcccctaatt
    13921 tttgagaact gacgtaatca atcaagtctt tggaactatc aaaggtctga tcattgactg
    13981 ctgtgactgt atcagagata ttgagaatcc ctttaaaggt tgaattatcc gtcacattca
    14041 aaacataaac tccaaagtac ttgagttcga tatccttacc agctgttttt agtccttgat
    14101 acttggccat attttgcgat gtttgcatgt agaattgatt gattcgcata aattcaacat
    14161 cggaagaacc acctgtagtc tcctgagcac tacgaatatc tgtaaaaggt gtcaaccaag
    14221 cataaatcat atgagctaaa gtggcatgtt gaacaccaac cgtaacgaat tgataggcac
    14281 cagcttcctt atcttctgtg tcatttattt taaggacttg gcgaatatct tccgaaccac
    14341 ctggaacctc tatataataa ggcaagggca ctacaaatgc caagaaagtc acaatcaagg
    14401 ccgcaatgac gtataagggc catctaatct tttttttcat ttcttatttc ctccaaaata
    14461 ctctcgggaa catagcaggc aatatcctga ccaaacttca aaagctctct aacgcctgat
    14521 gaactgatat agagatgttc aggtcgacta tgtaaataaa tagtctctat atcagaagac
    14581 agctgatgat tgtagtaatc aaaactggct tcatattgca aatccgacgc attcctcaaa
    14641 ccacgcacta gacaagtagc acccaatctt tttgcaacat cgaccaccaa ttcatcatga
    14701 gaagccacga cttcaacatt ttccagatgt cccaaagcct tttctagccc ccgtttacga
    14761 ttttcgatag gaagaaatcc ttgtttgtgg ggattaaaaa aaataccgac atagagctta
    14821 tcaaagagtc tgctcgcccg ttcaatgata tccagatgcc catttgtcat cggatcaaat
    14881 gagcctgtga ataagccaat cttatctgac atagactgtc accttactaa ttccataaat
    14941 cttttccttc cagataccca gacaggcaat ttcttctgga agttcaacgg ccttatccgt
    15001 ctcgcacaca accataacat cttcagaaaa aagctctctc tcagccattt tttcaatatc
    15061 tgctacgatt tgttccttgg tatagggagg gtctaagaaa acgaggtcaa attccccaga
    15121 tacctgttcc aatgcccttt ctgcatccat cttgaggagt tgaaattttc caatttcctt
    15181 ggttatctgg atattttcag ccacgatggt ctgagcctta cggtctcgct ccaccaaaac
    15241 agcactggac atgccacgcg atactgcttc gatagataaa ccaccactac ctgcataaag
    15301 gtccaagact cgtccccctt caaagtaggg accaatcatg ttaaaaatgg ctcccctaac
    15361 cttatccgaa gtaggtcttg tcgtcttgcc ttctagtgtc ttgaggggac gtcccccata
    15421 gattcctgat acgattttca tactgtttat tataccaaat tatagacaaa aagagaaaga
    15481 aaaccgaacc ttgcggttcg attctctaca aaatattttc gtaagtatcg cggacttctt
    15541 gaggccaaac acttgtttgc acttctccga tgtgtctctt gcgaagtagg aacatggcca
    15601 tacgagattg tccaattcct ccaccgattg tcaatgggaa tagaccattc aacaaagact
    15661 tgtgccattc caattccaag cggtcttcat cacctgtaat ttccacctga cgtctaagag
    15721 tttcttcatc tacacgaatt cccatagaag acaactcaaa ggctccacct aaagactcat
    15781 tccagacaag aatatcacca tttagaccct tgtagccatt ctcagactcg cttgtccagt
    15841 catcatagtc tggtgcacgt ccatcgtgcg gtttaccatc tggcaactcg ccaccgatac
    15901 caatcaaaaa gacggctcca aattctttac aaatcgcatt ttcacgttct ttcggtgtca
    15961 agtctgggta gcgttctacc aattcttctg tatggataaa ggtgatttgt tttggcaaga
    16021 tagactcgat gtcatagcgg gcttcaacag ctagctcagt caggcgaata gccttataaa
    16081 tcttctcaac tgtttctttt agataaacga tgttacgctt accatttggg ataaccttct
    16141 cccagtccca ctggtcaaca taaacagagt gggttgcatc caaggaatcc tcatctggac
    16201 gaagggcttt catgtggaca aagagaccct ctccttcacc aaagccaaaa cgagccaagg
    16261 tgtggcgttt ccatttagca agtgagtgca ccacttcata agtagcatca gggatttgga
    16321 gaaccttgac cgatactgga ttttccacac cagacaggtt gtcctgcatt ccgtcaccga
    16381 ccttactcaa gataggacct tgaacttcga caacttctag cttatctttc aaatactggg
    16441 taaaagtgtt tttgacaaag gaaatttctt cttgttgatg gataaaactt ttcttcatag
    16501 acaactcctc aaaaatttaa taaacgtatt atacacctat ttccaagaaa agaaaagaga
    16561 aaatttaaaa aaatcagtgt cactcaaaac actgatttca tagacctttt agtcattgcg
    16621 accaaagata cgtagaatac ttaggaaaag attgataaaa tcaagataga tgctgagagc
    16681 cattgacacc acccaacctg tcgctacacg accttgtgat tgttcatagg cgaggcgaat
    16741 cttttggtta tcccaagcaa tcaaccctga aaaaaccaaa accatggcta cgctaatcat
    16801 ataatcaaag aagccactag ccaagaaaat attgactacc atagcaatca gtaaaccaat
    16861 gagagctgcc atcattgctc gaccaatccc actcaagtct ttcttggtaa agataccaac
    16921 tgccgccatg acaaagaaga gaagggcgct cgatacaaag gcagataaaa ctgtacccgg
    16981 agtatagaaa gccaccacaa aactaagggt aaagccgttt aaaacggagt aaagtaaaaa
    17041 tactggaaga gcagctggac tatttcttga agccatgcta ctggcaacga aaaccaaagc
    17101 caactccgca aaagtagcaa tggtcaacca gagacgcccc tgcatcaaaa agtaaaccaa
    17161 ctgagactga aagaccgtca acataaggcc tgataccaaa gcagatagtc cgattcccag
    17221 accaacaaag gcataaacct tagcgtaaaa ttgattgaga cctgcacggt catgtataat
    17281 agtgtgattc atccatttct ccttttctgt gaacatcttt ccttgattat aacaaaaaaa
    17341 gttaaaatta gcttaaatgg aagattaaaa taccggctgc aaccgcaaca ttcaaactct
    17401 cggcctgccc cttcatgcta atatggacca actggtctgc actttcagcc ataaggggac
    17461 taattccttg accctcattt cccataacta gtacaaaatt ttctatagaa ggcagttctc
    17521 tgtaatcaac agaatcttta gatagggttg ttgctagcac tgggatagct acctttttag
    17581 tttcgtcaag aagcgcttga ctagtcatcc ggtaaatggg cagatggaaa tgactacctt
    17641 gcatggaacg tagggttttg agactgtaga tgtctgccga cttatctgaa acaatcactc
    17701 ctgtaaaacc tgctgcatcc gcagttcgaa tgatagttcc tacattacca ggatcttgca
    17761 catcttccaa aaacaagaac ttgccctgac tcaaatcagc ttgtcctact tcttctcttt
    17821 gaaccacggc aacaattccc tgtggagtct gagaatccgc caaatctagc aaaatatcct
    17881 ctgacaccca gacagtttgc gaaaaagtag ctaactgatc tcgataattt tctagggtaa
    17941 agattttctc aatcgtcact ccagcttgaa cagcttcttc aaacaagtgc cagccttcaa
    18001 tcaaataggc tgacttgcgg tatttttttt ggtgtaattt cttggtattt tttaccacag
    18061 aattggcttt tgaggttata atagtcatag aaatattata acacaatcaa aggggtttgg
    18121 tatgcaaaag gttagaatga ttgcccaagg tagggtacag ggagtcggct ttcgttgggg
    18181 tgtttacagc ttggcccttg aaatcggtgg catcacaggt cgggtatgga ataacgacga
    18241 tggcacagtg gaaatcttag cccaagcaga ctcatctgct atcatggcaa aatttatcca
    18301 agaaatccga aaaggaccga cacctttttc aaaagtaagc tacttagatg tcaaactaag
    18361 caactttcct ccctactctg actttaaaat cgcaaattag gtctccagaa ctattgtata
    18421 ttttgtaaaa aaacagtaga atagaaaggt ataattttta aagaaggaat aaaaacagtg
    18481 aaatctatta aacgttttgc attctcggct atgggagtgg ctatgttgct tgtcttgact
    18541 ggctgtgtca atgtcgataa aaccacaggt cagccaacag gatttatttg gaatacgatc
    18601 ggagcgccta tggctgaagc catcaagtac ttcgctactg ataaaggtct aggctttggt
    18661 gtcgctatca ttatcgtaac cattatcgta cgcttgatta tcttaccact tggtatctac
    18721 caatcatgga aggcaacgct tcactctgaa aagatgaacg ctctcaagca tgtccttgag
    18781 ccacaccaaa cacgtctcaa agaagcaact actcaagaag aaaaactcga agcccaacaa
    18841 gccctctttg ccgctcaaaa agagcacggc atcagcatgt ttggtggtgt aggatgtttc
    18901 cctatcctcc ttcaaatgcc tttcttctct gctatctact ttgctgccca acatactgaa
    18961 ggggttgctc aagcaagcta cctaggcatt cctctaggtt ctccaagtat gattttggtt
    19021 gcctgtgctg gtgtccttta ctatcttcaa tcgctccttt cacttcacgg ggtagaagat
    19081 gaaatgcaaa gagaacaaat caagaaaatg atttacatga gcccactcat gatcgtcgtc
    19141 ttctccctct tctcaccagc cagtgttaca ctttactggg ttgtcggtgg tttcatgatg
    19201 attctccaac agtttatcgt caactatatc gttcgtccaa aacttcgcaa aaaagtccgt
    19261 gaagaactag ccaagaaccc accaaaagca agtgctttct ctaaaccaag tggacgaaaa
    19321 gacgttaccc ctgaacaacc aactgctatc acaagcaaga aaaaacacaa aaatcgcaac
    19381 gctggaaaac aacgttcgag ataagaagaa aaaggcttag ctgatttgct aagtcttttt
    19441 gcaaatcaaa agcccgatgt ttccatcagg cttcttttct atccatgtac acgtttcata
    19501 taatcttgat aactttcggt atccatgagt tttttagcgt tcttgacgcg atctgctgtt
    19561 ggtggtttga ctccttcgag ggaatatgga attccaagtt cacgccactt gaactcaccc
    19621 atggtgtgat aaggtagaat ttcaaactta tcaacatttt tgagggtctt gacgaactta
    19681 ccaagttcaa tcaagtcatc atctctgtct gtcaatcctg gaactagcac gtggcgaatc
    19741 cagacaggtt ttccaatatc tgatagatac tgggcacaag ccaagatatt tttattagtt
    19801 tgtctagtga caatcttgtg ctgttcttcg ttgatttcct tgatatccaa aagaaccaag
    19861 tcagtgacag ccatgagttt gtcaaacttc tcaaggtaac gtggtttatt acggaaagga
    19921 agagcacagg tatccaaggt acagtggatt ccttgttcct tagccttggt gaagagagca
    19981 atcaggaaat caatctgcaa gagagcttct cctccactga ctgtaatccc acccttattt
    20041 ccccagaaac cacggtagcg caaggcctct gtcaagacat catctaccgt ccgttcacgt
    20101 gacttattgg actccatagc ccaagtgtct gggttgtggc aatactggca acgcatgtga
    20161 cagccctgca aaaagacaat aaagcgaata ccaggcccat ctactgaccc aaagctttct
    20221 gtcgaatgca ccattcctgt cacttgtcca taatcaattg tttcttcaga catatcgtta
    20281 ccttccttga aaacgtttta tagttttatt atatcatgac ttgaaggaaa aacaagattt
    20341 ttgcctattt tttagaaagc aatctgtttt tcaatttcat tttcaatcta ttatcagatt
    20401 attcttcaaa atcgaagcca tataaggttg caaaatagtc ctcagggcgc tctgcacggc
    20461 ggatttgacg ggctttacct tcttcggtat agaggatttc cgcagaacgt aatttggcat
    20521 tatactggta gcccattgaa aatccgtggg cacctgtatc atgaatgacc agcaaatcac
    20581 cgatttctgt atgaggcagt tcgcgattga ctgcaaattt atcattgttt tcacagagtg
    20641 aaccaaccac atctaccgct tcagctggtc catctggatg ggtcacgttg ctaatatgat
    20701 ggtaagctcc gtacatagct ggacgcatga ggttgactgc tgaggcatcc acacctagat
    20761 aggtacggta ggtttccttc ttatgagtca ctcttgtgac tagagcaccg tgaggtgcca
    20821 gcataaaacg acccaattcg gtgaaaatct tgacctgacc aagacctgct gacgtaagaa
    20881 cttcttcata caccttacga actccctcac caatcaaggc gatatcgttc ggctcctggt
    20941 ctggacgata attaacacca ataccgccag aaagattgat aaagtctagc gaaatgccca
    21001 acttttcctt gatttcaaca gccagttcaa agagctgacg agccaactct ggataataga
    21061 gatgggtcac ggtattggac gctaggaagg agtgaatccc aaaagtcttg gctccttttt
    21121 ccttcaagat agcaaaggct tcaaagagct ggtccttggt catgccaaac ttagcctccc
    21181 caggattgtc cataatgtct gtccccagtt caaaaacgcc tccaggatta taacgacaag
    21241 agatgatttc tggaatgcct gctactctct ccagatgttc aatatcttca aaggcatcca
    21301 agttaatggt cgcacccaat tcacgtgcat aggcgtattc cttgtctggc gtgttgttgg
    21361 aagagaacat aatctcagaa cccagaaagt ccagtttatg gctcatcaaa agctctacat
    21421 aactagagca gtccacacca caaccttctt cttggagaat tttcaaaata gctggagttg
    21481 gagtagcctt aactgcaaaa tattccttaa agcccttgtt ccacgaaaaa gcttggttga
    21541 cggctcttgc cttctcacga atccccttct catcatacaa gtgaaaggga gtcgggaact
    21601 cggcaacaat cgcttctaac tcttctctat tgataaatgg tgttttcata agcttccttc
    21661 tattctgttt gcaaagaaag gctgggacaa tcgttccaac ctttagttct atctcttatt
    21721 tatcttcgtc aaagatattg ccaacctcaa catcgatggc ttggttcttg acatcaatat
    21781 tagttacctt caagagtgac ttgtagtcaa actgcttttc acgttcttct tgggcattgt
    21841 ccgcaaagac cgctacacct gccagttctg agtcgaactc gcgcaagaga ctaatcatac
    21901 cgttgaccgt tccgccacct ttcaagaagt catccacaat caagacacgg ctgcctgcct
    21961 taagactacg ttttgaaagg aacattttct cgatacggtc accacttgaa cctgaaacat
    22021 agttgacgct aacagttgaa ccttcggtaa ttttcaggtc acggcgcaca atgacaaaag
    22081 agacattgag gacattggca actgcatttg caagtggcac acccttagtt gctacggtca
    22141 taaccgcgtc aattttttgg tccataaagc ttttggcaat aatacgacca atatttttca
    22201 agatggctgg tgtgctaagc aaatcagaca gatagatata accacctggc aagatacggt
    22261 cactttctga caacttggta cgcaagtctt caaccatttc cttagcatcc tgactcgaaa
    22321 tagacggtgt gaagatgaca cctccgccag ccccagtcac tgtctggata tgaccgattt
    22381 caatttcctc aaaggcgcgt ttgataatga cgatatcttc tgagatggat gatttagcag
    22441 actcatactt ttcagcaaaa gtattgagac tagttagttt ataaggatta ttaatcaaat
    22501 agttggaaat gacaaccatc cgatcacttc ttcttaattt catttctatt tcctattcca
    22561 tttttattca aaaaatcaaa aagcaaacta gaaagctggt cgctggtggt tcaaaacact
    22621 gttttgagat tgtcgataga actgacaaac cctgtaatat acctgcatat atacatacga
    22681 caaagcgata ctaccctagt ttgaagatat tttcgaagag tattcatttt tgtcttttac
    22741 ttattatacc atattcacat aaaaaaacga acattcttat cctaaaaaat gctcattttt
    22801 cttaaattat caatctaaat ctggtttata gaaggaacga ttatccatag cgaagatttt
    22861 attggtcatc tctcctttat ccaccaaagc cagagctgtt gacatcatca tcatgcttgc
    22921 atccagattg tcaatcatat ggataatctc tgcctccata atacgtggac ggactggact
    22981 tccatattca agcaagccgt ggtgactgag gatgacatga cgaagcaaaa cgacttcttc
    23041 cttggtatca tcgatgccga gttccataac tgtcttggta atttcgctat caatgagagc
    23101 gatatgtcca agaagattac ctcgcactgt gtactctgtc tggtctggcc ccgtcaactc
    23161 gatgacctta gccaagtcgt gcaacataat ccccgcatag agcagactct tattgagctg
    23221 aggataaact tcgctaatag cgtctgccaa acgtaccatg gtcgccgtat gataagccaa
    23281 ccccgtttca aaggcatggt ggttggtctt ggcagctgga taggagtaga attccttatc
    23341 atacttggtg tagagatttc ggacaatccg ttgccagaca ggattttcaa ttttgaaaat
    23401 catttgcgac atgtagtcac gaatttcctt gacatcaact ggtgacttga ccttgaaatc
    23461 ggctgggtca ttgggttcac cagcttgagg caggcggaga gtaatttgat tgacttgagg
    23521 ggtattgtta taaacttctc ggcgtccttt catgtggaca accttacctg cggtaaaggc
    23581 ctcaatgtta tgaggttggg catcccagag cttcccatca atctcgccac tatcatcttg
    23641 gaaggtaaag gctaggtagt ttttcccagc tcgagtttgc ctcaggtcag ctgatttgat
    23701 taggtaaaag ccttcaaata gctcatcttt tttcatgtga ctaatcttca tattcttcct
    23761 cattttcttg aaaatggagt agatcaagcg caggctcgcc ttctgacaac tcaatgtgac
    23821 ggagcgtccg ctcgatagct atggtacgac ggtttaataa ttcatcaata ttgccagagg
    23881 catgttggag atgtttttgt gccttgacca gaataccacc aaacttgcca aactcggtct
    23941 tgacactggc aagagtcttg ctgatatggt cggcactctt ttggatatta agggtcttga
    24001 aaccaactga tagggagtta agaagggctg atagggtact tggtcctgca acaataatct
    24061 gttcttcccg tctcaaatca tcaaagaaga ccggattgcg gacgatttct gagtagagac
    24121 cttctgtcgg aacaaacaaa actccaaaat tggtcgtccg aggtggtgct atgtacttgt
    24181 tcctaatatc cctagcaaaa cgcttgacac ttgctaggag tgacttacga cagcgttcga
    24241 tttcatcctt gtcacctact tcataggctt cttctaagcg gtaataatct gccagtggaa
    24301 acttagagtc aattggtagg tagacgtatt cttggtcgcc ttgtccgggt aacttgatgg
    24361 catactccac tcgttcacta gagttttcaa ccgttgcgta ttctcgttcg tactgggcag
    24421 gtgtcatgat gtcttcaata atttgcccca gttgcaattc tcccagaatc cctcgcgtct
    24481 tggttccaga gagaaccttg ttaagagctc cgacatcacg ggcaactgtc tgcatttctc
    24541 caagaccacg attgacagac tccagttgtt tagaaactgt ctcaaaggaa gcctgtaagc
    24601 gtgtctgcaa ggtcttttct agtttttcct cgaccgtctg gcgcatttgt tccaaacgtt
    24661 gctcatttga ttcctgcaag gcttggagac gttggtctgt cttgtctcta gtttggagga
    24721 gattatctgt catttcttga cggacttggg tcaggccttg gtgcaattca atccgcactt
    24781 cttgcaaacg gtcgctgaca gccacttcca aatctttttg gtctaactgg ctggcttgtc
    24841 tggcttggtc aaagcggtaa tccaactggt ctgacaaatg atctgcctga tcctccaagc
    24901 tcttacttaa gtgtttctcc tgcctatcct gtctttgcca aatcagaaag agaccagcta
    24961 ggttggcaat taataataga ataagtaaac tttccatcct acctcctgtc cttgctatgc
    25021 agtacgacca cataaccatc tgggcaagtc accgacactt ccctatctat atattcgtta
    25081 gaagcgtaca cttttttaaa gaaaaaattc tcctctgtca actcatactt agctccaaga
    25141 atagtcagct ggctatcccg aactggcata aaggctagat agtcgtagtc tgaacgaggt
    25201 tctagctgac tggtcccttc tgaacaatag gcaatcaaat tttgcccatc ctcaatttct
    25261 atttgacgca tatagggtgc caacttagga ttgctaggta gaaagacatt ggccaacata
    25321 tggtcaatac ggccacccaa ggcaccgaaa atagtgacct cagcctgagg attttgttca
    25381 aagatggtta agagagccaa ttccagatct gtatcatcct tttctggtcg ggcttggaca
    25441 aaatgctggg cacgtttttg aatcacctgt cgctcttctt ccgtcacaga atcaaaatcc
    25501 ccaactgcta gagcaagaag caagtcttct tccaagaccc agagcgagcc tcgatccacc
    25561 ccaacaaaag catcaaaatc tgtccgataa tgaccgcggt ctccgcctgc aaaaacggca
    25621 accctagtcc agttgttttc tgagagtttg tactcgctca ttgacatctc ccttaaagac
    25681 ataggaacct gctacaaaaa cggtcgcacc agcttcttta gcttgggcaa tagtttggtc
    25741 atcaatccca ccatccactt cgatttcaaa gttcaaacct ttttcctcac gaagagcaac
    25801 caactcacga accttatcca tggtttctgg cagaaaggct tgcccaccaa aacctggatt
    25861 aaccgtcatg actaaaactt ggtcaactag atgaaggacg tgcttgatgg cttcaactga
    25921 tgtgccagga ttgataacga ctgaaggctt aactccgagt gaacgaattt tttggagggc
    25981 gccatgaata tgaggcgttg cttctacatg gatactgatg atgtctgcac ctgcacgcgc
    26041 aaaatcttcc agatgatgct cagggtttga caccatcaag tggcaatcga aaaccatctt
    26101 actatgagga cgaaggctct cgaccacacc tgcaccaaaa ctgatttgcg gtacaaaatg
    26161 actgtccatg atatcgatat gggcatattc tgccccagtt gcttctagac gtttgatttc
    26221 acgttcaaag ttggcataat ctgctgccag aattgacgga gcaatcttgt attgagacat
    26281 aggtttctcc ttattttgga atttttttgc tgactttttt ataggtttct ctacgatttt
    26341 caatttcact aaggaattgc aggtaattgt caaaacggaa ggttgcaata acaccctctt
    26401 caacagctgg tttgacggca caagacggct catgggtatg ggtacaagta cggaacttac
    26461 aatctcggct aacagtagca atctctggga aagcctgatt gaggtcttca gcccttgata
    26521 cttcatagtc caaggatgaa aatcctggtg tatctgcgat tttacccccg ttgagattgt
    26581 aaaaactaac agctcgagtg gtatggcgac cgcgacctag actgtctgaa atttctcccg
    26641 tttcaagatt gaggtctggt gcgattttat tgagaagagt tgacttccca acacctgtct
    26701 gccccataaa gaccgtaacc ttgcctgtta acaaagacag gagttcctct ttactggtca
    26761 caaagtcata gccgatgtca ccataggtct gctggtaaaa atccagttct cccctatctt
    26821 ccaacaaatc cattttggaa atatagacaa tgggatggat gcccttgtgc tccaaaagaa
    26881 ccaagaaacg atccagcaaa ttgctgttaa aatcaggttc cttgacggac atgattacta
    26941 cagcttgatc gatattgaca ataggcggac gaaccagact gtttttccgt tcgtgaattt
    27001 tgaggatata gccttctgaa ttttcctcgg cagagaaatc tacccagtcc ccaacataag
    27061 gggtatggcc ttttttacgg aaattcccac gcgcgcgtgt ttgataaacc tggccatcac
    27121 tctccacata gtagaaacct gccaaggctt taatgatttg tccctgcatc ttagagtcct
    27181 tgccctttaa gtgcgtctgc tagaccggca aattctgcca agctgagagc ttccccacgc
    27241 acacttggtg acaagcctgc ctggtccaaa gccttggtca gcttgtcctt gacctcttca
    27301 gtcttaccaa agtaacctgt caagttattc cacaaggtct tgcggcgatg ggtaaaacta
    27361 gccttggaaa ccttaaagaa aaagttctca tcttctactg ctacggctgg ctctggacga
    27421 cgcaccattt tcaagatggc tgaatccaca tttggcgctg gcacaaagac cgtacgaggc
    27481 acgataaagg caaccttggc tgtcatgtaa tactgcacgg cgatagacaa gctaccgtaa
    27541 gccttggtgt taggctgggc tgaaatgcgg tccgctactt ctttctgcat catgaccaca
    27601 aactcactaa aaggaatgcc actctcaatc aagtgcatga gaataggcgt cgtgatgtag
    27661 taaggcaaat tagccactac cttgattggc aggttaggat ttttaaaatt ttggatatgt
    27721 tgcgccaaat caaccttgag aatatcttcg ttaactacag tcacattatc aaaatcacgc
    27781 agggtatcta ccaaaattgg caccaaacgg tggtcaatct caaaagccat gacttgggct
    27841 gcacgctcag ccagaaactc cgtcaaggca ccaatacctg gaccgatttc gatgacattg
    27901 acctggtcat caatttcagc cgtatccaca attttttgaa ggatattggt atccgtcagg
    27961 aaattttgcc cgaaggactt tttaaaggta aaaccgtgac gctccagcac tgccttggtc
    28021 acgctataat ctgcaattct catctattct cttttctatt atctgttact actattgtaa
    28081 cacattcccc ttgtattttg ggaatgaaaa tcataccaaa aaactatctt tcctcatgaa
    28141 gaaagatagt cttggaaatc tttaaaagtg gccttaacag ttctctcgaa acatgcgttc
    28201 atactcttcg aaaatctctt caaaccacgt cagcttcacc ttgtcgtact caagtacagc
    28261 ctgcggctag cttcctagtt tgctctttga ttttcattga gtatcaaaaa aatcagcttc
    28321 ttgataactt ctattttcgg gtagggtcaa atccacatcc tcctccacat catccactgt
    28381 cagttcaacc gtttgaggta aatttttcaa acgataatgc ttttgccttt acccttcaat
    28441 actagcaaaa tcaagcaaat agccatgcac taggatttga accaaaagga gatgattggt
    28501 catattttta tcttctaaac cacctctggc actaggataa tcagataccc tgcgaatacg
    28561 gattttttca cacatgagca agtttcctca tatcaatgat ttcatcagcc ttattccaga
    28621 ctaggggatt atgggtcgca atgatgataa ttcgattttc atctttcaaa tccaccaaga
    28681 gattcataac ctcctctgaa ttttcaggat caagagctgc tgttggttca tctgccaaaa
    28741 tcaagggtgg atttttcaaa atagtttttg caagggcaac tcgttgggcc tctcccccag
    28801 ataaagtata gattttttgt tctaaatcca aataccctag attaactttt tctaaagccc
    28861 ccacttgcct ttccaaacgt tctacttttg agattttctg accaacaaaa cccaaatcca
    28921 aattttcttt gattgattgg ttttctaaga ggccgaaatt ttgaaagaga tagcccatct
    28981 ggtctcgaaa atactcacga gtaggaatgg tttttaaatc tttcccctga tagagaaccc
    29041 ttccaccatc tatcttttct agctttccca agatattcag cagcgtcgtc tttccgcttc
    29101 cactctttcc gattaaggca taaaccttgc ccttctccag cttgagattc aaaccagaga
    29161 aaatcgcgcg gtcattaaat ttcttttcca atccttgaat atcaatcatc ttattttcct
    29221 ttcaatactg ccatgctacc agcttcttct tttttgtcct gccttactaa aatcaagagg
    29281 gcgttaagcg taaacaaagc tacaactaga gcgctaatca aaccatctct tgtcaaaata
    29341 ctagatagga ctaggccaag caagagaact cctccttgcg ccagtaaata cttgccatga
    29401 agttcataga ttgtcatacc agcaagacgt ttaatcataa gctcccgtct gaactgctca
    29461 aaatagagaa gattcatgga atcaaataac aagatagccg tagacagggt caaaatcgtc
    29521 ccaataagga gagagtaaaa ctccacccgt ttgtcattta gtaccttggc aaaaaatagc
    29581 tggcttttta ccaagtaaga aaccttgtga tacagacctt gctcttttag agctgtgatg
    29641 ctgtctccat atcgatctag tttcaaggca ctattagccg tagttcccca caacatccct
    29701 gcaacaggtc ttgttccagt cgcttgcggc gttagaacta caataatcgg atcttttagg
    29761 tactggcgtg ttgtcttgta cccatcatta tagaggaaac gttcctgtcc tgtttctgta
    29821 aaagctagcc ttacctgtgg gaggtagtgt ttctgactcg tcacttctac actttcagat
    29881 gaaaagtttt gcaggtaatc tgtaaacaat ccttggtagt agacagactg ctctcgcaag
    29941 ctctcaggca agatcagccc aaactctccc tcagacaagt tttgcatctt gtccataaac
    30001 tctgaagaaa cggtaatctt ttcttctatc agatagcgtg gtgagacata gataacattc
    30061 cctgacggtg tgtagtcact gagacgattg ccatctaggt ccacttctgc tccatctgag
    30121 aaattgtcaa cattgctcat aatataaaaa gagtctgtat tggctaaccg ttcttcagta
    30181 aatgtctgcc actcacgatt atccttacgc gttccttctt catcggcaaa tgcactagac
    30241 caaccaaagg atagacggta acggtctgag gactggctcc atttattgct agctctctcc
    30301 atttcctgca tttcacggta gtggggtagg agagctgtcg cactcgatcc gaccaccaat
    30361 acagctaaga gttgccccac catcatcaat gtcatcatac gtttgagagg gagtttccct
    30421 ttcaatagat ccaccagact attttcctgt aaaccaagta gatagacgac acttagtaag
    30481 gtagaaatcc ctgccaaggt caatccataa agtagaagag caatgatgac cagttgcacc
    30541 gttgccataa acaaggcacc ttgataccag agaatcccca atcccaaaag actggatacc
    30601 agcactgagc agataagctg tctcacatct tctaacactg gtctgagagc aactccaaac
    30661 aagctctcac cagctattaa gcgaatccct gcctgacgaa gggatttgat ccgataaatc
    30721 agggtcagac tcataaaggt cagcagaaaa atagctaaac tcagtagcac cataggggtg
    30781 gccgtcaata gtaagactat cgaaaatgga tcttctccat tcgaaacaaa gccttgataa
    30841 ccaagctctt tcaaggtggt ctgtaagctc actccatcca aacttcctga cacaatcaag
    30901 taagacccta ataaatcact cgtttctgca ctctccttgg aggaaatggt cagaccttct
    30961 ggaagctttc cctcaccata agtggcatag gtaaagcgtg tttctccagc ttcatttggc
    31021 tcaacgattc gacgagcaat gagactattg tgctctaccg ccaagtgact caaagcctct
    31081 gtcacctctt cccgactagc tactggttga tttatcatca ttcctgtcac atcaacagaa
    31141 ggatagttac caaataaaat ctcttctcga tggtcaacgg aagttatcac catataaagc
    31201 gataccaata ccattgaaat caaaataaat aaacgtttca tcatttcctc caaaaagtta
    31261 cttggtcact tagtgagaag gtgaccaagt aaagctaaac aatagggcta taccattcta
    31321 aacattatag tagaatgcaa ctttctctcc ccaagaagta cgaatccatg cacgagaagt
    31381 ccctccagca ccagcatatc ccttgtcaga tgaaccagtt tttctgctaa caactgttga
    31441 actatggtat tttgaagggt gatagtagtt tgagtaagca ccaccgttac ctgaaccata
    31501 agtccaaaca ccaccttctg ggtattgaac ggctgcagag gcaactgcta cacttccacc
    31561 taatgttgct gcaatgctcg ctacaagagc taatttttta actgtttgtt tcatattgat
    31621 actcctctat ttttgattac aagattgttg agttacttgc tcctctttcc attatcaacg
    31681 ttgatactat tatattacca aatgatattc taaaattatt tgtttttctc taaattgtca
    31741 aaatagcacc ctgaaatgaa cttttatagt cattatactc catctttatc acactatcaa
    31801 ggacatcaat gtccttaaaa tctaactatt tcatattatt tttagcttat atctaaaaaa
    31861 acagttctaa agacgaactt tagaactgtt ttgatacgag gacaaggaca tttttgtcac
    31921 tgattcacta tgtttataaa tattttttga catcctcttg ccattcctct gtcaatttct
    31981 ttactagaaa tccatctcca attagcttcg caaatgtttt agaagactga taaaatgatt
    32041 cagctaaact aaaatctctt tctactctta atgcttgctt ccacaaaaac ataaatacaa
    32101 gaggtttctt ttgataatct tgagttttat ccataatttc ttgtaaacac gatagataat
    32161 cattaaaaca atcagttact cctacctttt caagacaaca cattcctgaa ataagagtat
    32221 ctctaattat aaagtaattc tctatttgag ttaccttctt ctgttccaaa agaaagagca
    32281 ttagcttttc gatttgattt ataaatttgg caagatcttt tctaatgagc atttgataaa
    32341 aaaacaaacg aatcaacaac atatcattta ttgaataagc ttctttttct ataatctgag
    32401 gcatatgttc ctcaagtatt aactctgcat attcaggcct tctagttcta gaagtatcca
    32461 aacacgcctg caatacttca catgctaatt gttcttcttt aggaagatta tcaaagtatg
    32521 ttttataaat ctcttccaaa cacgcttcct tcttatcgta ctcctcttct ttaccataga
    32581 ttggttcacg taaaatctgg tatttcaatt ctaaataagc agaaggaaga gctgaaaaat
    32641 ccggcataag gctataaact ggaactccta gccggcgagc aatataggct aacttgggca
    32701 aactcggttg cgaagctcca ctttcaattc gcgacagttg acgaactgtc agttcttgct
    32761 catctccaca aaaatctgga cgactaattc ctttttctat ccgaatctct ttaatttttt
    32821 gaccaattaa catcagacgc ctcctcttct acctgcttcg aaaaatactg tgaaacttaa
    32881 aaaattgtaa cggctacatc tttcctactc ataagatttc atagcttctt ccacttctgc
    32941 caaagtaacc ccaaacaact ctaggcgttt gaggagttgc ttgccgttag aatagccgat
    33001 tcggagagtc tctccgagat attctctacg cttacggctg tctgcccctg ctagaaaacc
    33061 aaggcgaatc aaatcgctac gactaatgtc aaactgactc tcatgttcaa attgttctgt
    33121 cacctgagct agagccgttt tcaggtcttc atagctggca tgctcaattc ccagagaacg
    33181 ccccttggtc ttggacttgg gaacagcttc atctcgcttg agaaaggcat gctgaactgt
    33241 tggaatggcc gtcataatca tgcgccgaat ccgctcccca ttaaaatctg ggtctgtaaa
    33301 gacaatgact ccatgacgtt ggtgtagacg ctgaatccgc tctatgtcct ggtcattgat
    33361 ggcagaacct cgagtctcat aggtctccac atcgaaataa cgcttgagat tgaccgtatc
    33421 atcacgacct tcaaccacga taacttggga aattctctct ttcattactt gctgtccaat
    33481 ccaaaaatgc gttctgcatt tgcagtcgtt gctaccgcca gctcttctgt cgtcatacca
    33541 cgcaagtcag cgataaagtc gaccacatag cgagtatagg ctgttttatt ttcacgacca
    33601 cgcttgggta caggtgctaa gtaaggcgca tctgtttcta ccaacatctt gtccaaaggt
    33661 aactctttag ctgcttcttg gaggtcagtt gccttcttga aggtcaccac tcctgagaag
    33721 gaaatggtca taccaagatc cacaaacttc tctgcccact caagcgtccc tgaaaatgaa
    33781 tgcatgatac caccacgagg accaacgccc tcactcttga taatctcata ggtatcttcc
    33841 agcgcatcac gggtatggac aacaaaaggc aaatccaagt ccttagatag ccgaatctga
    33901 cggcgaaaaa cctgctcctg cacctctttg ggcgctgtca tccaatggta atctaagcca
    33961 atttcaccta aagccacaac cttgggatgt tttaacttat ccaacaagta agcctcaact
    34021 tcctctgtat aagtaccagc ttctgtagga tgccaaccaa tagtcgcata gagctgctca
    34081 tactcatcta ccaactccaa ggcacgctca atcatcggtt tatcaaaacc aacaatattc
    34141 atctgtatca cacccatctc agcagccaag gcaatttctt ctgcctcacg acctgcaaat
    34201 tcttctacat tcaagtgtgt atgtgtatca aaaatcatct cttctaacct cgttttctat
    34261 cttctattat accaaaaaag aggaggggca cctaattttt cggtttcccc tcctctcttc
    34321 aatagagagc tattctgcta tcttttctat ccgatattgc ccatctccta ttccacagtt
    34381 agagacagaa gagattggct acattgatag aatattccta ttcagctaaa gtatgtgatt
    34441 atcttgggtt ctcacttttc tcacccatca ctaggattga tgacttctca aaatccccct
    34501 tataatctaa ctttaagtag agaatagaat tttctagctg ttctattttc tacgtctagt
    34561 ctttcttcct agcaaagcaa aaccacttgt tgccaccact agaccaagac tagcaagtgc
    34621 tgcacttgat gctgaacctg tgttaggtaa ttgtttgcca gcaggtgact ggtaggtaac
    34681 atcttgcatt gttcctgcag ttgcttgcgg tgcacttgtt gtcttaggag cagcattagg
    34741 agccactagt ctcttaggaa ctgtaaccat ggcaatggct tgcactccaa tcgtttctgc
    34801 ttccttcaca tcttccatag ttttcgcatt ttcaatctct ttgagagctg cttgtttttc
    34861 tgcttccact cttgctaaaa gttctgcttt ttctttatca gaaagcggtg cgcctttgat
    34921 ttcatcctgc ttgtccttgg ctgctttttc aatggcattt ttagctgatt ctttttcagt
    34981 agctaattgc ttagaagctt gtgattgagc tgagttttgt gaacctgtgt gagctgatcc
    35041 acccgcctgg ctaccaccgc cttcattcgc attagcatct gagcttccaa tattgcctcc
    35101 cttatctcca cctggtgtag tttctagctt atactctggt gtaacttctt gagttacaga
    35161 ttcacctttc gcagattgtt gaactgtatc ttttcctaga atcgtcacta ctgaaccatc
    35221 tgggaatgtg attgttgctg taccatctcc agctacattg attgtcgctc cgtctaatgc
    35281 tgaaccattt gcttgtaaaa tagcaacctt aactttttct tcatctgtta aatgagttat
    35341 atcttttaca tctactcttt gagcaggtgc tttaacagtt gatttttcag cagtcggttt
    35401 agtgctatct tgtgcttttg gatcttgtgc taccaattga tttcctggaa ttgtaatagc
    35461 tgaaccatct ttaaagacga ttgttgctgt accatctcca cctacttcaa ctttatagta
    35521 aaataaaata ataacaggac aaatcgatca ggacagtcaa atcgatttct aacaatgttt
    35581 tagaaacaga ggtgtactat tctagtttca atctactata acatcgcttc cgaatccatt
    35641 tgcatctact gaactttttt acaaaaataa aaatcagtag actcgagttc ctactgattt
    35701 ttgatagaat tcgtttggat tatgcagcca aaactttgcg tcctttacga cgacgagctg
    35761 ccaatacgcg acgaccattt ttagttgaca tacggttacg gaatccgtgt ttgcgcgcac
    35821 gacgaagttt acttggttga taagtacgtt tcacgatgaa tacctcctca tagattttgt
    35881 attcgtttag ccggctataa agtgatcagt tactaaacat actttactat tctatctgat
    35941 tctttcactt ttgtcaatag ctctaggcaa atgtttccag ctttttcgaa tgcaagccct
    36001 atctacgata ctgtttcaag aaacgagtca tcttttcttg gatttgggct aactcatcaa
    36061 cacgaggaag gtaaacgata cggaagtggt ctggttcctg ccagttaaag cctcgaccat
    36121 gaaccaagag aaccttttcc tgcttcaaga aatcaaggac aaactgctca tcatcatcga
    36181 tacggtacat attgcggtcg atttttggga agatatagag ccccgccttg ggtttaacgg
    36241 cagacaaacc tggaatatct tgaatggcat tatagatgaa atttctttgc tcgtagattc
    36301 gtccaccagg aagaagcaat tcatcgactg attggtgacc ccccaaggaa gtttgtacga
    36361 cttgttgggc caaaacgtta gagcaaaggc gcatattgga cagcatattg agcccttcga
    36421 tatagccctt aacatgagtc ttagggccag acaagaccat ccaacccaca cggaaacctg
    36481 ctatgcggtg ggattttgac agaccattca tgctgacaca gaagacatct ggtgccaagc
    36541 tcgccacagg cgtatgcaca tgtccgtcca ttaccatgcg gtcatagatt tcatccgcaa
    36601 agataatcaa atcgttttga cgggcaatct caataatctc cagcaagagt tccttaggat
    36661 aaagggctcc agttgggtta tttggattga taaggacgat tgccttggta ttggaagtga
    36721 tttttgactt gatatcgtca atatctgggt accattcaac agcttcatca cagatatagt
    36781 gaacggcatt tcctccagct aggctgacag ccgccgtcca tagaggatag tctggcattg
    36841 gcactaagac ctcatcccca ttgtccaaaa gcccctgcat ggacatgaca atcaactcac
    36901 tgacaccatt tccaaggtaa atatcatcaa tatctacgtt tgggaatttc ttcagctggc
    36961 aatactgcat gatggcctta cgggctgaga aaatcccttt ggagtcagaa tagccttcac
    37021 tatcacgcgc attcataatc aagtcatgaa tgacctcgtc tggcgctgta aagccaaatt
    37081 ctgctggatt tcctgtattc agacgtaaaa tcttttctcc attcgctcgc atccgcatgg
    37141 cttcttccaa tacaggacca cggatatcat aagcaacatg ctctaactta ctagacttgt
    37201 tatattcttt catcttgttt cctcaattca tcaggatagt aacattatac cactagaaag
    37261 agaatgcgac aagtttactg aaatgtgtta ctagtttcgt atagaagaaa aaccttgcta
    37321 aaggcgaggt taagaacgta gagatagcag gtattttgca aagacacatc acataccaaa
    37381 agataaacct agaaaaagca agcctagaaa ggagtcataa agaagattaa acatgaggaa
    37441 aagcccccat atcatcaagt aatcccaacg cccacttcgt tttgcaacta gaaataagat
    37501 tagatggtag agtacatgaa agactaaaaa tccaataaaa cttggataaa gaagcggtac
    37561 cagactctct aattgacaga tcatgatcac ttttaccaag actgaaacta ggataattcc
    37621 ataataaagg aaatttgatt tttgaaaaga gtttttcatc attctccttt cctcctatcc
    37681 tttcttttat tttatcatat atttatatag agtttaagcc ctaatattct tttgaaaaat
    37741 actttttttg ctttacttct atagtgaaaa ggtttacaat tatagtaaga aaatttcagg
    37801 aggtttcatt atggcacaac gttaccaaaa tatcatggtc gcaatcgatg gttctaagga
    37861 agcggacttg gcttttgtca agggagttca ttctgctcta cgaaacgacg ctaaactcac
    37921 catcgcacat gtcattgaca cacgcgctct ccaaagcgta tccacctttg atgctgaagt
    37981 ttacgaagaa ctccaagtcg acgctgaaag tctgatgaaa gagtacgaaa aacgtgctaa
    38041 agatgctggg gtagcagatg ttcatatcgt cattgaaatg ggaaatccaa agaccctgct
    38101 agcacgtact attccagatg ccgaggaagt ggacctcatc ctcgtgggcg caactggtct
    38161 caacgccttt gaacgcctct tggtcggctc ttcatctgaa tacatactcc gccatgctaa
    38221 ggtcgatttg ctggttgtga gagaacaaga aaaaacctta taatcacaaa gaaaaggagc
    38281 ccctagctcc tttttgttta cgatttattt ctctctttat ggcgttcgta agccttgagc
    38341 tggcgatgca gttccttttt aatagcaggt tctggagcat atttttcttc ccaattatct
    38401 ggttttaaga ttttatgggt cactggatca aaatgagcct tgccatctgg aaaaattttc
    38461 cccatattgg cctgatggac aatatcaaaa atacgttctg ggtccacccc catcaagaca
    38521 aaactgccgt aggtgaagta aagcgtgtca atcaaggcat ccacttgccc tatcaaatct
    38581 tgctgagcag gtgtcttctt ggctacttta cctgctgcct tatcaagggc ctgatgaagt
    38641 tgcgatacag cttgaccaaa atcttcttca gaaggactgg ctgctcgaac aaactccacc
    38701 aattcttcta ttttaaaacc agccctatgg gttgcaccct ctaaatccca agctcgaggt
    38761 tcttcttggg ttcgttcatc catcatgtgg tggaaggtct tgaccttatt gaaatgatag
    38821 tcacggctga caaagacttt ttctgaagac aaaacaccaa aatgttccaa ggccttgtgg
    38881 atcccatctt gttgattgct agtggtaatg tgcttggcag cttccttgac actgctacta
    38941 ccatttccca tagcgaccga cataccgacg ccagccagca tttcaaggtc attatctgag
    39001 tcaccaaaag ccatgacttg gttgaggtca aagccatatt ctttcccaac tcggcgaatg
    39061 ccttctaatt tagaatttcc ctgattgatg acatccgatg caaaaggatt gctacgtgtc
    39121 aatttcaagt cttcaaaatc agctgccgcc ttctcagatt cttctggtgt catcagcatc
    39181 aaaacttggt agataggctg attcatcagg tgaagcaggt cctcttcctt ttggggaaca
    39241 accttgctga ccatgcgatt aaaagactga ctcaccgtcc gagttaaaac agagggaacg
    39301 aagcgactaa ttcgttggga aaaagaaccc agaccaaagg acatgatttt agaacccaac
    39361 atggcatcct tggtccctag agcaatctcc gtgccctctt ttttagcata gctaattaga
    39421 tggcgcaaat gtaacttgga aatagggctc gtgaacaaga ctctgtcttt actaaagata
    39481 tactggccat tataggttac cgcaaaatcc agatccaaat cgtccatcaa ttccttaaca
    39541 aaaaaaggtc ctcgccctgt cgctacgcca actagtaccc cttgttcttt gacaatctta
    39601 atcgcgtcct tagtggattt caaaacactc ttgcgattgt tgaccaaggt tccatcgata
    39661 tcaaaaaaaa cagctttgac ttccatccta ttccaatctc cccttttgtg atacaatgat
    39721 tataccacat ttcagaaaga gtgagtaaat catgcctaag aaaatccttg ttttacatac
    39781 gggtggaact atttccatgc aggccgatgc ttctggcgct gttgtgacga gttcagataa
    39841 tcccatgaac catgtgtcca acccacttga aggaatccaa gtccacacct tggacttttt
    39901 taaccttcca agtccccata tcaaacccaa acatatgctg gtcctctacc agaaaattaa
    39961 agaggaagca gataactacg atggagtggt gatcacacac ggaaccgata ctttagagga
    40021 aacagcctat ttccttgata ccatggaagt tccccatatg cctatcgttc taacaggagc
    40081 catgcgtagc tccaatgagc tcggtagtga tggtgtttat aattacctaa gtgctttacg
    40141 agtggccagc gatgacaggg ctgctgacaa aggagttttg gtcgttatga acgatgaaat
    40201 ccacgctgcc aagtatgtca ccaaaacaca tacgactaat gtcagcacct tccagactcc
    40261 aacacatggc cccctcggtc tcaccatgaa acaggaaatc ctctacttca aaacagctga
    40321 acctcgtgtt cgctttgacc ttgatcacat acaaggttta gtccctatca tctcggctta
    40381 tgctggtatg acagatgagc tgattgatat gctggattta gaacacttgg acggtttgat
    40441 tatccaagcc ttcggagctg gtaatattcc caaagaaacg gctcaaaaat tagaaagcct
    40501 tctgcaaaaa ggaattccag tcgctctggt atcacgatgc tttaacggta ttgccgagcc
    40561 tgtttatgcc taccagggtg ggggcgtaca gttgcaaaaa gcaggcgttt tctttgttaa
    40621 agaactcaac gcccaaaaag ctcgcttgaa actcctcatc gccctcaatg ccggactaac
    40681 aggacaggct ttgaaagact atatggaagg ctaatactct tcgaaaatct ctgcaaacca
    40741 cgtcagcgtc gccttaccgt atgtatggtt actgacttcg tcagttctat ctacaacctc
    40801 aaaaacatgt tttgagctga cttcgtcagt tctatctaca acctcaaaac agtgtgttgc
    40861 gcagccttta accagccgcc tagtccgctc tatggtattc attaagtcaa catctcttgt
    40921 ttaagagcac aaaatcagga aatcttctcg attccctgat tttttctatt tacgttttcg
    40981 tgttgagcta cgttctgtca aaccatgagg taagagaact tcacgttctt ccaactcttc
    41041 cttatgcata atcttggtca acatacgcat actaatggca ccaaggtcat aaagaggttg
    41101 ggcaatcgtt gtcaagtttg gacgggtaaa gcgtgagatt tgtgaatcat cactagtaat
    41161 aatttcaaaa tcttctggca cagaaacacc cttatcagcc aaaccgttca agactcctgc
    41221 tgccaactca tcacctgtca caactgctgc agttgcattt gatgaaatca aacgctctgc
    41281 taaggcgtaa ccatcatcat agctatattt agattcaaat accaaaccct cactataagt
    41341 gattcctgct tttttcaagg tttccttgta gccaactaaa cgaaccttac cattgatgtc
    41401 atccactagc ggaccgctaa cgaaagcaat acgctcattt tctttagcaa ggtaactcac
    41461 tgcatcaatt gttgcttgct tatagtcaat attgacactt ggcaactggt gctcaacatc
    41521 gacagttcct gcgagaacaa tcggagtacg tgaacgcgaa aattctgagc gaattttatc
    41581 tgtcaagtga taacccatat agataatgcc atctacctgc tttgaaaaga gggtattgac
    41641 aacagaaact tctttctcgt tatcttcatc gctattagct aggacaatat tgtacttgta
    41701 catttctgca atatcatcaa tccccttagc caaactcgaa aaataaccat tggtaatatt
    41761 tggaatcacg acaccgacag tggttgtctt tttacttgca agaccacgcg caactgcatt
    41821 tggacgataa tccaaacgat caattacctc tagcactttt ttacgggtat tctcttttac
    41881 atttttattg ccattgacca cacggctgac cgtcgccatg gaaacacctg cttcacgagc
    41941 gacatcataa atggttactg tatcatctgc attcattcct tttcctgtcc tttctatctc
    42001 acacattctt ttacaagtag aggtactgat tgaagctcta tatctactta caaaagtgaa
    42061 gatgtgaaaa tttcgttttc atatttctac ttattccatt ctatcactaa ttgtaaacac
    42121 tttcaagtgt tttttgaaga ttgattgaaa aaatttcata gaaaacctag gtttagctcc
    42181 ttgctaccac cttagactaa acaaaaagga ggaaactaag ccctcctaaa gttatagtaa
    42241 aatgaaataa gaacaggata aatcgatcag gacagtcaaa tcgatttcta acaatgtttt
    42301 agaagtagag gtgtactatt ctagtttcca tctactatag gtattgttcc attcactacc
    42361 gtcaatttta gcacatagtc ttcatgaaaa tattatatca tcataaccaa ccagattctt
    42421 ttgcgatatt agctgcctct gttcgattac cagcatccag ttttgaaaga atattggtga
    42481 catagtttcg aatagttccg tttgagaggt aaagctgatc tgcgatttct tggttggata
    42541 agccccgggc gattcccttt aagacagcaa tctcttgttc tgtcaacgga ttgggacggg
    42601 tcatcaccac ttccatcaat tcaggcgaat actccttgcg tccttcgagg acggtgtgca
    42661 aggtttgcat gaggtctgca atgcttcgtt cctttaatac ataagcatcc actccagcct
    42721 tgaccgcacg ttcaaaatac ccagcacgct tgaaggtcgt caccacagcc acctttgttt
    42781 caagcctttc tgatcgtatc cactccaaga cttcaagacc tgtcttaaca ggcatttcta
    42841 cgtcaaggat ggcgatatct acagactcct tttctaatag ttggattgct tcttgcccat
    42901 tcttggcttg aaggacagac tccacatctg gttgaagcgt gagcaattga cacatggcat
    42961 ctcgcaacat actttgatct tctgcgacta atactttcat cttagtttct ctccttataa
    43021 ggtagtcgaa cttgcacttc cgttggatgt ttctgactga ttacacttac ttctcctgaa
    43081 aatggaaaaa cacgatctcg gactgtatgg agctcatctc cttttagaaa agcaaagccg
    43141 cagccatcat cacttactgt taaaatgagt tccttctctg tccgttctaa ttttaagtaa
    43201 gctttagacg ctttggcatg tttgatgata ttggtcacca actcaagcaa aatcatagaa
    43261 gccattgaat ccaattcttg agtaaggcta gcagtatcta gttggttatc tgtttccacc
    43321 tcaattccag caatttctaa catctttttc acagtctcta gttcggatgt caaagttcta
    43381 gacttaagat tttccacaat ggttcgaact tcacacattg attctttgct aatttgctgt
    43441 atttctctta attccttttc cacttgtgga taagcctgca tttgaaatag ctgcaaggct
    43501 agatctgtct tgacactaag catagcaaag gtatgtccca gactatcatg caaatcctga
    43561 ccgatacgat tgcgctcatt ttctgctagc aatagattta tctgggtatt ttgcttgacc
    43621 tgagcttctt tcaaatcctc gacaaggcga attcgaaccg agcccaaagt catcaaatcg
    43681 acaaaagtaa gaattacaag catatagact agaaactcaa cttcgattct ctgaaaaatc
    43741 aaaagtcgcc ccacaacaag gacttgagca aggagaaatg tccatacatg taaagacttt
    43801 aaactacgta cgccaaaatg ataaattaag agattggaaa ggaaaaagaa aaaccagata
    43861 taattcccag caacaaaggc ggtattccca gctacataga ccagcatgag cccccagaaa
    43921 agccaagata ggcgctggtt cttagttatt aaaacaccca aatacgtcac tacaaataga
    43981 atatcaacca ataaatgcca agcaggaagc tccccaatca ctacaggtag gatgggaaaa
    44041 agcataaaaa tcaaactggt ccaaaacata tagtgtatgc ttttcagtct ttcaagcatt
    44101 aagcattctc cttatgacct tgaaggtaaa tagtcaaacc aaataaaact gctgaaaaaa
    44161 caagtaaata aactgtggct gatagattga tgtcaccctc gtttaagaag gtcttgagca
    44221 actccatcaa ttgataggtc gggagacact taccaactgc ttgcatccag tctggaaata
    44281 aagaaatggg catccagagt ccgcctaaaa cagccaatcc tagataaaga agattgccca
    44341 caacagacat cagctgacta gttggtaaga gagtcaaggt caagccaagt gctacaaaag
    44401 caatactccc cacaatcagc aaaatcgcag ctccaaccca ttttactaga ggcatgtcta
    44461 cacctcttac aaaatgtcca actgtaaaaa caaccagaat tgagaccaaa taatcaacca
    44521 gcatacttgt tatctttgat agataatatt ctaccatatt tacagggcta tgacgcaatg
    44581 ttttctgcca gttgttcatc ttatcggtat gtaaaacagc tggaaatgag aggatagttg
    44641 tcgacatcat ggaaaaggca gtcatggaga tgaggtaatc acgcataaaa ttcgcgagtt
    44701 cacctggtgt gtcctgatag ataccagaaa aaaataaata gaaagccgtc ggcatcccta
    44761 ccgacaatag ataatagatt aattgtcgtt tggtcaataa aaattctatc ttactaagtg
    44821 ctatccatcg tttcatctta gttatctccc ttctgcgtct cttcaaagat tgtattcaac
    44881 aaactacgat tattaacttc gatttcttgc atcctacatc ctgcttgggc taatagttcc
    44941 cagaaagcat ctgcttctcg agtgactact tgtaaagaat cttgctttag ggtccagttt
    45001 tcaaccaaat ttgactgctc tacgacttcc ttgtaagcta taggaaggat aaagtgcttt
    45061 tctatttcct cgctacgcat agctagaggc gtcgtatctc gaatcaactc tcccttattt
    45121 aagagcaaaa tgcggtctgc agtgtgttcc acttcctcaa tataatggga cgaatagaga
    45181 atggtaactc cctgcgcttt tagatcctga acaatttccc aaaaacgttg gcgggttgag
    45241 gtatccatgg ccgcagttgg ctcatctaaa aagacaagtt ttggtcggcc aatcaaggtc
    45301 aagacaaaag agaaaagacg cttttgccca cctgacaatt tttctgccaa ttgctctttc
    45361 tgttgcttgt caaactgcaa cagttgatcg atttcctggt tgctcaaggg atttggataa
    45421 atgctttgaa agaaagcaac caactctttg acctttaatt tctgaacgat gacattttct
    45481 tgaggcagat aacctctaat atagtctaac tgagaactcg tcactgacaa gccttggatg
    45541 gatacttgac cgcttgtgac cagtttatct ccaagcagac agtccaagag tgtggtcttc
    45601 ccagcaccat ttggcccaat caaggcgacg cattcacctt cagctacctc aaaggaaata
    45661 tccttcaaaa tagcctttcc cttgatgttt ttatgtaatc cttgaacctt aatcatgtcc
    45721 atgatattct cctttcaacc actccattcc cataggaaag gcgataaaaa tcataaatcc
    45781 tagtccccag gcaccacgga tgaattggcg aagcaaggct tgatcaaacc aacctgtaaa
    45841 catttctacc aaccatacca ctagtgatag tccaataaaa agatagagaa acgctttttt
    45901 catttttcaa actccttttt cacatctgag actaatttca aaccttctcg gataagccag
    45961 gacatcattc caaatcctgc aaatagctcc caaggaaaat gatagaaatc ttcatccaat
    46021 cctgagaaca tgagataagt catgactcct gctgctacta aactcattgc gacaatcatt
    46081 ttatttttca tctcttcttc ctccgtttca tacttcaatt atagactttt gaagttggcg
    46141 gagctagatg cttctgtcac tagaaaatat gacaaatgtc ataaaaattc tgtacaaaac
    46201 aagcaagata cactatataa taaaacacaa gtagaaaaaa tctaaggcag ctttcaaaag
    46261 agatatcaaa cccaattcac accataatgt aaactaatac ttatttaaaa tcaaaaagag
    46321 cggaaatttt tatcgcacaa acacatatat tataagcaga gcgaaaaact cttcaattac
    46381 tttcttaaaa gtttgtaaga attatacaat ctgttaaatt ttaatttatg tacctaaact
    46441 acagtattca ttgaacaaat taggaaacaa aaaagtatac gctgctaaaa tgaattactg
    46501 aaattcaaaa aagagagaag caaactgatt ctctcttaat gtatataata tctagttaaa
    46561 acaaaaagat acgcttacgt atctctgaaa
//
LOCUS       DAVLRT010000007        44412 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLRT010000007 DAVLRT010000000
VERSION     DAVLRT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.007"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            98..865
                     /locus_tag="VXM85_000367"
     CDS             98..865
                     /locus_tag="VXM85_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1344998.1"
                     /translation="MIGISLIPALYNIIFLSSMWDPYGQLSDLSVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSKKTTTLSNI
                     QSTAAYQSLTSEQQTGISDSVSQNSTDSIQSAQSIVALVQGLQGSLENLQNQSSNLST
                     LKNQANQVLPITSTSLIGLSSGLTEIQGAVTSKLVPASQLIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            950..1357
                     /locus_tag="VXM85_000368"
     CDS             950..1357
                     /locus_tag="VXM85_000368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1344999.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNIIFAKLPSGRH
                     PESRWPWLKSRAEINGIIAVLAGILVYGGVLLV"
     gene            complement(1727..4279)
                     /locus_tag="VXM85_000369"
     CDS             complement(1727..4279)
                     /locus_tag="VXM85_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1345000.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQALKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISITSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTYKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4341..5963)
                     /locus_tag="VXM85_000370"
     CDS             complement(4341..5963)
                     /locus_tag="VXM85_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1345001.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIAPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            6169..7194
                     /gene="trpS"
                     /locus_tag="VXM85_000371"
     CDS             6169..7194
                     /gene="trpS"
                     /locus_tag="VXM85_000371"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1345002.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            7346..8824
                     /gene="guaB"
                     /locus_tag="VXM85_000372"
     CDS             7346..8824
                     /gene="guaB"
                     /locus_tag="VXM85_000372"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1345003.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSINEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(8875..9972)
                     /gene="recF"
                     /locus_tag="VXM85_000373"
     CDS             complement(8875..9972)
                     /gene="recF"
                     /locus_tag="VXM85_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1345004.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSKSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(9975..10343)
                     /gene="yaaA"
                     /locus_tag="VXM85_000374"
     CDS             complement(9975..10343)
                     /gene="yaaA"
                     /locus_tag="VXM85_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1345005.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            10502..11752
                     /locus_tag="VXM85_000375"
     CDS             10502..11752
                     /locus_tag="VXM85_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1345006.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            11749..13032
                     /locus_tag="VXM85_000376"
     CDS             11749..13032
                     /locus_tag="VXM85_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1345007.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13074..13895
                     /locus_tag="VXM85_000377"
     CDS             13074..13895
                     /locus_tag="VXM85_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1345008.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            13906..14451
                     /gene="pgsA"
                     /locus_tag="VXM85_000378"
     CDS             13906..14451
                     /gene="pgsA"
                     /locus_tag="VXM85_000378"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1345009.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            14448..15275
                     /locus_tag="VXM85_000379"
     CDS             14448..15275
                     /locus_tag="VXM85_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1345010.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            15260..16099
                     /locus_tag="VXM85_000380"
     CDS             15260..16099
                     /locus_tag="VXM85_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1345011.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            16092..16886
                     /locus_tag="VXM85_000381"
     CDS             16092..16886
                     /locus_tag="VXM85_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1345012.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            16945..17763
                     /gene="mreC"
                     /locus_tag="VXM85_000382"
     CDS             16945..17763
                     /gene="mreC"
                     /locus_tag="VXM85_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1345013.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            17763..18257
                     /gene="mreD"
                     /locus_tag="VXM85_000383"
     CDS             17763..18257
                     /gene="mreD"
                     /locus_tag="VXM85_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1345014.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            18351..19529
                     /locus_tag="VXM85_000384"
     CDS             18351..19529
                     /locus_tag="VXM85_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1345015.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            19753..20532
                     /gene="rpsB"
                     /locus_tag="VXM85_000385"
     CDS             19753..20532
                     /gene="rpsB"
                     /locus_tag="VXM85_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1345016.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGHQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            20611..21651
                     /locus_tag="VXM85_000386"
     CDS             20611..21651
                     /locus_tag="VXM85_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1345017.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEDGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            21897..22220
                     /gene="tnpB"
                     /locus_tag="VXM85_000387"
                     /pseudo
     CDS             21897..22220
                     /gene="tnpB"
                     /locus_tag="VXM85_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(22361..22648)
                     /locus_tag="VXM85_000388"
                     /pseudo
     CDS             complement(22361..22648)
                     /locus_tag="VXM85_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22639..23037
                     /locus_tag="VXM85_000389"
     CDS             22639..23037
                     /locus_tag="VXM85_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1345018.1"
                     /translation="MKAPIPKAPLAHSFGSASIIAHTIHQKFNLKVPNYRQEEDWAKM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(23234..24154)
                     /gene="cysK"
                     /locus_tag="VXM85_000390"
     CDS             complement(23234..24154)
                     /gene="cysK"
                     /locus_tag="VXM85_000390"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1345019.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLVLAPDNGERYLSTALYEL"
     gene            complement(24439..25074)
                     /locus_tag="VXM85_000391"
     CDS             complement(24439..25074)
                     /locus_tag="VXM85_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1345020.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            25130..26428
                     /locus_tag="VXM85_000392"
     CDS             25130..26428
                     /locus_tag="VXM85_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1345021.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            26425..27087
                     /locus_tag="VXM85_000393"
     CDS             26425..27087
                     /locus_tag="VXM85_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1345022.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            27167..27715
                     /gene="raiA"
                     /locus_tag="VXM85_000394"
     CDS             27167..27715
                     /gene="raiA"
                     /locus_tag="VXM85_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1345023.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            27851..29824
                     /locus_tag="VXM85_000395"
     CDS             27851..29824
                     /locus_tag="VXM85_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1345024.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQIIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            29821..30273
                     /gene="rplI"
                     /locus_tag="VXM85_000396"
     CDS             29821..30273
                     /gene="rplI"
                     /locus_tag="VXM85_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1345025.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            30316..31668
                     /gene="dnaB"
                     /locus_tag="VXM85_000397"
     CDS             30316..31668
                     /gene="dnaB"
                     /locus_tag="VXM85_000397"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1345026.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            31670..31936
                     /locus_tag="VXM85_000398"
     CDS             31670..31936
                     /locus_tag="VXM85_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345027.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            32025..33371
                     /gene="cbpD"
                     /locus_tag="VXM85_000399"
     CDS             32025..33371
                     /gene="cbpD"
                     /locus_tag="VXM85_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1345028.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      33454..33556
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33627..33917
                     /locus_tag="VXM85_000400"
     CDS             33627..33917
                     /locus_tag="VXM85_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1345029.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            33880..34638
                     /locus_tag="VXM85_000401"
     CDS             33880..34638
                     /locus_tag="VXM85_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345030.1"
                     /translation="MRNLRSILRRHISLLGFLGVLLIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            34677..35684
                     /locus_tag="VXM85_000402"
     CDS             34677..35684
                     /locus_tag="VXM85_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1345031.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            35684..36409
                     /locus_tag="VXM85_000403"
     CDS             35684..36409
                     /locus_tag="VXM85_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345032.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLE"
     gene            36532..36990
                     /locus_tag="VXM85_000404"
     CDS             36532..36990
                     /locus_tag="VXM85_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1345033.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            36992..39424
                     /locus_tag="VXM85_000405"
     CDS             36992..39424
                     /locus_tag="VXM85_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1345034.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            39745..40398
                     /gene="rr06"
                     /locus_tag="VXM85_000406"
     CDS             39745..40398
                     /gene="rr06"
                     /locus_tag="VXM85_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1345035.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDEITQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            40395..41726
                     /gene="hk06"
                     /locus_tag="VXM85_000407"
     CDS             40395..41726
                     /gene="hk06"
                     /locus_tag="VXM85_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1345036.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIYE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            41809..42336
                     /locus_tag="VXM85_000408"
     CDS             41809..42336
                     /locus_tag="VXM85_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1345037.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            42415..>44412
                     /locus_tag="VXM85_000409"
     CDS             42415..>44412
                     /locus_tag="VXM85_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEV1345038.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSKN
                     NLTVTSSGQDISKKYADEVESHLQSILKDVNKNLKKVQHTQNVDFNKKLSRIKTKYLY
                     GLKEKSEAELTLKTKETKEELTAAFEQFKKDTLKSGKKVAEAEKKAKAQKEEDRRNYP
                     TNTYKTIELEIAEAEVGVAKAELELEFAQAQVQIPQDTEKINAAKSKVEAAKSNVKKL
                     EKIKSDIEKTYLYKLDNSTKETPKPRVRRNSPEIKAKGRVKNYEEANIELSKYMTDLY
                     KLDNSTKETPKSRVRRNSPQVGDSRELKETIDKAKETLSTYMVTRLTKLDPSVFWFAD
                     LLMDAKKVVEEYKTKLEDASDKKSVEDLRKEAEGKIESLIVTHQNREKENQPAPQPGG
                     QAGGSMVVPPVTQTPPSTSQSPGQKATEAEKKKLQDLIRQFQEALNKLDDETKTVPDG
                     AKLTGEAGKAYNETRTYAKEVVDKSKKLLSQTAVTMDELAMQLTKLNDAMSKLKEAKA
                     KLVPEVKPQPENPEPKPQPEGEKPSVPDINQEKEKAKLAIATYMSKILDDIKKHHLKK
                     EKHHQIVALIKDLDKLKKQALSEIDNVNTKVEIENTVHKVFADMDTVVTKFQKGLIQN
                     TPQVPEAPKSPEVPKVSDTPKAPDTPQVPEAPKSPEVPKVPE"
BASE COUNT        14109 a         7243 c         9548 g        13512 t
ORIGIN      
        1 tctattatat caaaatttta gacaatatgt ttaaaaaagg agaaactatg tttaaagaat
       61 ggaaagcaat ttaaaaaaaa caacctttat tattgtcatg atcgggattt ctcttattcc
      121 agctctgtac aatatcatat ttttgtcatc aatgtgggat ccatatgggc aattgtctga
      181 cttatctgtg gcagttgtaa ataatgataa agaggcttcc tataatggta atactatggc
      241 aataggaaaa gacatggtgt ccaatttaaa agaaaataaa accttggatt ttcattttgt
      301 agatgaagag gaaggaaaga agggattgga agatggcgat tactatatgg tagtgacttt
      361 accaagtgat ttatctaaaa aaacaactac attatccaat attcaatcga cagcagctta
      421 tcaatcattg acaagtgagc aacaaactgg gataagtgat tctgtatctc aaaattcaac
      481 tgatagtatt caatcggctc agtcaattgt agctttagta caaggtttac agggaagttt
      541 agaaaactta caaaatcaat cttctaatct ttcgacttta aaaaatcaag ctaatcaagt
      601 attacctatt acttctactt ctttgatagg attgtcaagt ggattaacag agatacaagg
      661 agctgttact agcaaattag ttcctgccag tcagttgatt gcatcaggtg taaacgcata
      721 tactacaggt gttgataaag tttctcaggg cgcaagtcaa ctaagtgaaa aaaatgccac
      781 cttgacaggt agtttggata aactagtttc aggctcaaac accttgacac aaaaatcttc
      841 tagattgaca gcaggagttg gttaattaca atcaggatct gggcaattag cagacaaatc
      901 cagtcagtta ctttcaggtg cttctccatt agagaataga gctaataaat tggcagatgg
      961 atctgggaaa ctagcagaag gtggaacaaa gttaacttct ggattggaag atttacagac
     1021 aggacttgct tctttaggac aaggactagg taatgctagt gatcaactca aatcagtatc
     1081 aacagaatct aaaaatgcag agattttgtc aaatccactc aatctttcaa aaacagacaa
     1141 tgatcaagtt cctgtaaatg gaatcgcaat agctccttat atgatatcag ttgctctttt
     1201 tgttgcagca atatcaacaa atataatatt tgcgaaattg ccttcaggac gtcatccaga
     1261 gagccgttgg ccttggttga aatctcgagc tgaaataaat ggtattatag ctgttttggc
     1321 aggaattttg gtatatggag gagttctatt ggtttaactg ctaatcatga gatgagaata
     1381 tttattctca tcatccaaac aagtttagta ttcatgtcta tggtgaccac tttagcaacg
     1441 tggaatagcc gtataggagc ttttttctca cttattttgc ttttactata gttagcatca
     1501 agtgcaggta cttatccact tgctttgaca aatgatttct ttagatctat taatccctgt
     1561 ttaccaatga gctattcagt ttcgggatta cgacaaacaa tctctatcaa caagtcattt
     1621 tcctagctgt catactagct ctatttactg gtttaggtat gctagcctat caacataaga
     1681 aaatggaaga agattaaaaa aatcgaccga ttaactggtc gattttttat gccttagatg
     1741 actttcgtct gtgattatag attccaaata gtaagagaga agtaaaggaa cagattgctc
     1801 cagtaataaa accattggga atgaaggaaa gtgtaatagt tcctttcccc ttgggaatgt
     1861 caactttcat aaatccagtt tgagcttgtt taatttctat tttcttacca tcttggtagg
     1921 cagaccaacc tttgtcataa ggaatggtga agaaaataga tgtatcttgt tggacatcat
     1981 atgtagcaaa aaccttgttt ttataagttg atactgtgac aggttgttct ttaatttttt
     2041 gaattgcctc ggtgaaagtt ttggtatcta aacgatagaa ggtaggagat tcaaatgata
     2101 cttgtgaatt tccagggaaa ctaacattga tattgaaagt ttttttctct ttagtatatc
     2161 ctagattaaa gaaggagaag acattatcag ttgtaaaagt ctttttttca ccatttacaa
     2221 ggatgtcaac cttcttttgt ttatcgttag aaaagtgaag gtttatgaaa gagagataaa
     2281 cttggctgtt ttctggaact tcaatttgat actggattgc tgcatcttca tttgaagaac
     2341 ttgtgatact aatcaaatca ttagtatttt ctattttttc tgttttttca taaggtattg
     2401 gagaaaaata atcaaaattg acgttagcaa gttgatttaa aaatgaggcc tgattatcca
     2461 aggtatgttc attgaacttg acatcattgt aaacagattg actagcaact gcaatcggaa
     2521 gagagtattg attttcatat agggtaagat tatctttttg atagatatct ttaaagccat
     2581 acttatcaat aggactgtct gagatattgt actggatacc aaataaacta tcagccaaaa
     2641 tactattatt tgcatatcgg agattgagat tagtcccaga ggatttaaaa ccaagtttat
     2701 ctaaagtaga gcttgatgaa cgatttcgaa cagatgaaaa ttgagagatt ccattgtagt
     2761 tgaatttcat actgtcattt cctgtctgag tttgtagttt ttcagtacga gtaaattgat
     2821 ttccaatata tgttgagaaa gattccatag ctgggatatc tcgactataa gcacttcgag
     2881 aagcaaatcc ccattcctta gcaattccgt ccatttgaga tgaagcattt aaactcattt
     2941 caaccagtat aaataaagag attagaatgg caaatagatt cacagatata aactttttga
     3001 taactgcaag gagtaaaaga gaatagacaa ccaaaaattc aagagtaagc agaatattca
     3061 aatctgttaa aaaagaataa tgcgatttta gatagatggt agctaaaaat cctgctacta
     3121 caagaaaaag cgaaactaaa aaattccaga ctttaagttc tttcagacgc tttaagactt
     3181 ctgctgctgt gtaaattaac aaggtagaga aaatccaagc atagcgatgt aaaaacatgt
     3241 ttggagtatg catgccttgc caaaataagt caagagcttc tatgtaaaag cttgcaatta
     3301 gaaatgcaaa gaatattaca tatatgagtt tcacgtgaaa cttaatagat ttcagcgtaa
     3361 aaaataaaat ggtcaaaata aagggaaata gtccaacaaa aatcattggg atggccccat
     3421 actttgttgt gtcaaaggaa ccaatgaatt gcttagcaaa gagatcaaga taccagctac
     3481 tttcagtttg aaactttgta acttcagtca atttttcccc atgtgtctgt aaatcaaata
     3541 gagtgggaag agtcataatc aaactagcca taccagctaa aaaggagata actatgaaat
     3601 caagaacaga tgattttcga gtcttaaagt cccacgaaat ttgacagaga taccagaaaa
     3661 taagaaacaa tactgtcata tatccaaaat aataattttg aataaataag attgacagac
     3721 ttgtaaagta caataggagt ttcttttcag ttatcagtag atgtaaacca gttataatta
     3781 aaggaatcaa gataaaaaca tctagccagg tttttatctc taattgactg acagtgaaac
     3841 tcatcagagc ataggaagta gataaggcta gttttaaagc ctgagggata gattgaaaca
     3901 atttattcaa actaaaaaag gttgacagac caatcaatcc aaattttaag agagttgtca
     3961 gatagatagc atctggcata ttcgttagat caaaaaagta aaccagaggc gcgagaaaac
     4021 tacccaagta ataactagat agggcataga agtttagccc tagaccactt gtaaaggtgt
     4081 aaaacagact actatttcca tgtaggatat ttcgtaaggc tacatcaaaa ataacgtatt
     4141 gatgaaagcc atctcctaat agaggagagt tgtcgctatt ccagtagata ccttgagata
     4201 gatatactcc agacataatc actacaggaa tgatgaaaga aataaaatag gttcgatatg
     4261 tttttaaaaa tgatttcatg ttacctcgta gaatgataga aaactcagtt ggttaaccca
     4321 actgagtttt gaagttttat ttagtctttc caaagttctt taacttttgc ttgtacttct
     4381 gcattttcta ggaattcatc gtaggtttca tcgatacggt caatgacgcc atttttagat
     4441 aagacaatga tatggttagc caaagtttga ataaattcgt ggtcatggct ggcaaagatg
     4501 attgattctt taaagttttt caatccatca ttcaagcttg agatagattc caagtccaag
     4561 tgatttgttg gatcatcaag tacaaggaca tttgatttta agagcatgag ttttgaaagc
     4621 atgacacgaa ctttttctcc ccctgacaag acatttacag gtttgttaac ttcatctcca
     4681 gagaagagca tacggccgag gaagccacgt aggaaagtat tgtcatcttc ttctttactt
     4741 gcgaattgac gcaaccagtc aagaattgat tctcctcctg caaaatcagc tgagttatct
     4801 tttggtaggt aagattgact agttgtaact ccccacttga cagttccttc atagtcaata
     4861 tctcccatga ttgcacgaat taatgcagtc gtttgaatat cattttgtcc aataagtgct
     4921 gtcttatcat ctggacgcaa gatgaaacta atattatcca agatagtttc accatcaatc
     4981 tttacagtta gattttctac tgtcaagaga tcattaccaa tctcacgttc cgctttaaag
     5041 ttgataaatg gatatttacg actagatggt gcaatctctt ctagctcaat cttatcaagc
     5101 attttcttac gtgatgttgc ctgccttgac ttagaagcat tggcagagaa acgagcaacg
     5161 aattcttgca attgtttaat tttttcttct gctttagcat tacggtctgc tagcaattta
     5221 gcagcaagct cagaagattc cttccagaag tcgtagtttc cgacatagag tttgattttt
     5281 ccaaagtcaa ggtcggccat gtgagtacaa actttgttta agaagtgacg gtcgtgggat
     5341 actacgataa ctgtgttatc aaagtcaatc aagaagtctt ctaaccaagt aatcgattgg
     5401 atatccaaac cgttagtagg ctcgtccaag agaagaacat ctggtttacc aaaaagtgct
     5461 ttggcgagga gaacctttac tttttcaccg ttggccaatt cgctcatgtt ttggtagtgt
     5521 aattcttctg gaatgtttag gttttgaagt agttgagagg cttcactctc tgcttcccaa
     5581 cctccaagtt cggcaaactc tccttcgagt tcggcagcac gaaccccgtc ctcgtctgag
     5641 aaatcttcct tcatgtagat agcatctttc tctttcatga tgctataaag tttttcattt
     5701 cccatgataa cgacatcaat ggcacgttca tcttcgtagt caaagtgatt ttgacgaaga
     5761 acagagagac gttcatctgg accaagagag atgtgaccag tagtaggttc gatatctcca
     5821 gctaaaattt ttaaaaaggt tgattttccg gcaccattag caccgattaa tccgtaagta
     5881 tttccttctg taaatttgat attgacatca tcaaaaagtt tgcgatcact aaaacgtagt
     5941 gaaacatcag atactgtaag caatattttt ctcctatatg tgtaatatat ttattctact
     6001 agaaaataca gaaatattca aatttttatt tgtcaatttt gtgtaaatta tatttacagt
     6061 atcctttaca caaatctgta aaaagcaagg ctgatttatt ttgataaatt acggttattt
     6121 cattaaaaaa atgctataat tgaaaggact atatcgaagg agaacaaaat gactaaaccc
     6181 attattttaa caggagaccg tccaacagga aaattgcata ttggacatta tgttggaagt
     6241 ctcaaaaatc gagtattatt acaggaagag gataagtatg atatgtttgt gttcttggct
     6301 gaccaacaag ccttgacaga tcatgccaaa gatcctcaaa ccattgtaga gtctatcgga
     6361 aatgtggctt tggattatct tgcagttgga ttggatccaa ataagtcaac tatttttatt
     6421 caaagccaga ttccagagtt ggctgagttg tctatgtatt atatgaatct agtttcgtta
     6481 gcacgtttgg agcgaaatcc aacagtcaag acagagattt ctcagaaagg atttggagaa
     6541 agcattccga caggattctt ggtctatcca atcgctcaag cagctgatat cacagctttc
     6601 aaggctaatt atgttcctgt tgggacagat cagaaaccaa tgattgagca aactcgtgaa
     6661 attgttcgtt cttttaacaa tgcatataac tgtgatgtct tggtagagcc ggaaggtatt
     6721 tatccagaaa atgagagagc agggcgtttg cctggtttag atggaaatgc taaaatgtct
     6781 aaatcactaa ataatggtat ttatttagct gatgatgcgg atactttgcg taaaaaagta
     6841 atgagtatgt atacagatcc agatcatatc cgcgttgagg atccaggtaa gattgaggga
     6901 aatatggttt tccattatct agatgttttt ggtcgtccag aagatgctca agaaattgct
     6961 gatatgaaag aacgttatca acgaggtggt cttggtgatg tgaagaccaa gcgttatcta
     7021 cttgaaatat tagaacgtga actgggtcct attcgtgagc gccgtattga atttgctaag
     7081 gatatgggag aagtttataa tatgattcaa aaaggtagtg aaagagcgcg tgaagttgcg
     7141 ggtcaaaccc tatctgaggt aaaaggggca atgggacttc attactttaa ttaagtttat
     7201 tttacaagaa actatcattt ctatctcgaa gaaaagatag ttttttattt acccctgtaa
     7261 catttgacaa atttttatag aatggtatga tagatacaat ataaaaagag tcaagctcaa
     7321 aaagaaagaa aagaggaaac ttcgaatgtc taattgggac actaaatttt tgaaaaaagg
     7381 ttttaccttt gatgatgtat tgcttattcc agctgaaagt catgtgttgc ctaacgatgc
     7441 agatttaaca actaaattgg cagataattt gactttaaat atcccaatta ttaccgctgc
     7501 catggacaca gttacagaga gtcaaatggc cattgctatt gctcgtgcag gtggtctcgg
     7561 agttatccat aaaaacatgt caattgctca acaagcagac gaggttcgta aggtaaaacg
     7621 ttctgaaaat ggagttatta ttgatccgtt cttcttgacg cctgaacata caattgctga
     7681 agcagatgag cttatgggtc gttaccgcat cagtggtgtt ccagttgttg aaacacttga
     7741 aaatcgtaaa ttggttggta ttttgacaaa ccgagatctt cgttttattt cagattataa
     7801 tcaaccaatt tcaaaccata tgactagtga aaatcttgtt actgctcctg tgggtacgga
     7861 tcttgcaacg gctgagagta ttcttcaaga gcatcgtatt gaaaaacttc cgttggtcga
     7921 tgaagaaggc agtctttctg gtttgatcac tatcaaagat attgaaaaag ttattgagtt
     7981 tccaaatgcg gctaaagatg aatttggtcg tcttctagtt gcaggtgcag taggtgttac
     8041 ttcagataca tttgaacgtg cagaggctct ttttgaggca ggagcggatg cgattgttat
     8101 tgatactgca catggtcatt ctgcaggtgt cttgcgtaaa attgccgaga ttcgtgctca
     8161 tttcccagat cggactttga ttgctggaaa tattgctact gctgaaggtg cacgtgccct
     8221 ttatgaagcg ggtgtagacg ttgttaaggt tggtattgga ccaggttcta tctgtactac
     8281 tcgtgtgatt gctggtgttg gtgttccgca agtaacagct atctacgatg ctgcagctgt
     8341 tgcgcgcgaa tatggtaaaa cgattattgc tgacggtggg atcaagtatt ctggagatat
     8401 tgtaaaagca cttgctgcag gtggaaatgc tgttatgctt ggatctatgt ttgctggaac
     8461 tgatgaagct ccaggcgaaa ctgaaatctt ccaaggacgt aaattcaaga cttaccgtgg
     8521 tatgggatca attgctgcta tgaagaaagg ttcaagcgac cgttatttcc aaggttctat
     8581 caatgaagca aacaagctcg ttccagaagg aattgaaggt cgtgttgctt ataaaggagc
     8641 ggcagctgat attgttttcc aaatgattgg tggtattcgc tctggtatgg gttactgtgg
     8701 tgcagctaac cttaaagaac tacacgataa tgctcaattt attgaaatgt ctggtgctgg
     8761 tttgaaagaa agccatcctc atgatgtgca aattactaat gaggcaccaa attattctat
     8821 gtaaaaaaca atgaaaagaa ctccagtgaa aacaggagtt cttttacaat gttgtcaatt
     8881 tccatttaca gcagctttac catcctgaat agtgaagata cttagatttt ctggtagatt
     8941 ttgaagatgg tctaagcttg ttgttgtgat aaaggtttgg attgattgag aaatcgtttc
     9001 taataatttt aattgtctag tgttgtcaag ttcactcatc acatcgtcaa gcagtaatat
     9061 aggagattct gtggtaatgc tttccattaa ttcgatttct gctaatttta tcgagaggac
     9121 gagactacga tgttgacctt ggcttccgaa actagcatcc atcccattta tataaaaaga
     9181 aatgtcatct cgatgaggac cgacaccagt attcttttta aataaatctc tggatttact
     9241 tttttctaaa gcaattttga aagattcgga taagttttgt ttgtcagtta tattgacaga
     9301 agattgatag gatattgaca actcttcgat ctgattagag agttcaaaat gtttcttacg
     9361 cccaaatgat tctagttttt ttatgaaatc taagcggtga ttcattacac gacatccata
     9421 atcaactagc tgatcatcta acacagaaag gaatgtttca tctatttttt gatctgattt
     9481 taggtaagtg tttctttgct ttaggatgtg gttataattg gttaagtcag ataagtagat
     9541 tggcttaatt tgcccaagtt ccatatcgat gaattttcgt cgaatcgaag gtgctccttt
     9601 aattagttgt aaatcttcag gagcaaataa gacaacattc atgtgtccta cataatctga
     9661 aaggcgtgcc tgttttaagt gattaacttt tgttacacgg cctttttgtg ttagttcgat
     9721 ttctagggga atggatccag tttttttctg aacgagacct gaaagatgaa gttgttcctc
     9781 atcaaaatga atgagatttt tatctgttcg agttcgatga ctacgcgtta aggctaaaaa
     9841 atagatagcc tctaacatgt ttgtttttcc ttgtgcatta cgtcccaaaa agacattcaa
     9901 tttaggatta aagtctattt tcgtttcttt gtaattacga aaagtcttga gagatagatg
     9961 ttgtagccac atgattatct accagggaat cgtggagctt gttggctttt aggtgatgaa
    10021 gcaggtttcg atttgtcttt tttaactccc ttattcatct ttttgacaag tttagcgatc
    10081 cgttcttttt caactttatc agcttggtat tcatcttgtt cttcagaagt aggttgtgtc
    10141 aacaagatgt caatgttcat gtcagggatg tcaactttat cgccaatacg aagttttttt
    10201 ccacgacgac tctctaattc cctattaaag taaacagaat gttcagagag aaatgattta
    10261 atagctcctc cgctatgtgt aattccaagt tctttgagta gtgcttggag ggtaataaat
    10321 tcttcaaata atttgtattc cataattcac ctcaatcttt ggtattatac catattttcc
    10381 taaaaatagt gagagagaca gcgagaaatg taagcgattt ttatttcaaa agtgttacag
    10441 ggaacaagaa aatttcaggt tttcgtgata taatagaagt ctgtatataa ggaggtaaat
    10501 catggagtta gtgcatggaa tttcaacaca ttttatccaa tcaaaaaagt ttaaaacaaa
    10561 caaaattacc gtgcgtttta ccgctccatt atcccttgat acgattgcag gtcacatgtt
    10621 gagtgcaagt atgctagaga ctgctaatca gatgtacccc acttctcaag atttgaggag
    10681 acacttggcc agtctatacg gtacagatat gtcaaccaat tgtttcagaa gagggcaaag
    10741 ccacattata gaattgacat ttacctatgt tcgtgatgag tttttaagta ggaaaaacgt
    10801 gctaacctct cagattttgg aacttgtaaa agaaactctt ttttcacccg tagtagttga
    10861 taatgggttt gatccggcct tatttgaaat tgagaaaaaa caattgctag caagtttagc
    10921 agctgatatg gatgattctt tttattttgc acataaagaa ttggataaat tgttttttca
    10981 tgatgaacgt cttcaattgg aatatagtga tttacgaaat cgtattttag ctgaaactcc
    11041 acaaagttct tactcttgtt tccaagaatt tttagccaat gatcgaatag atttcttttt
    11101 cctaggtgat tttaatgagg ttgaaattca aaatgtatta gaatcatttg gctttaaagg
    11161 tcgaaaagga gatgtgaagg ttcagtattg tcaaccttat tctaatatcc ttcaggaagg
    11221 tatggttcgg aaaaatgtgg gacaatccat tttggaatta ggttatcatt accgttctaa
    11281 atatggtgat gaacaacatt tacccatgat tgtaatgaat ggtttacttg gtggatttgc
    11341 tcactctaag ctctttacaa atgtccgtga aaatgctgga ttagcttata ccatttcaag
    11401 tgagcttgat ttatttagtg gattcttgag gatgtatgct ggtatcaatc gagaaaatcg
    11461 taaccaggct cgtaaaatga tgaataatca actgcttgat ttaaaaaaag gttattttac
    11521 agagtttgag ttaaatcaga ccaaggaaat gattcgttgg tcgttgttac tttctcaaga
    11581 taatcaatct tcattgattg aacgtgctta tcaaaatgcc ttatttggaa aatcttcagc
    11641 agactttaaa agttggattg caaagcttga acaaattgac aaagatgcta tttgtagagt
    11701 agctaataat gtgaaactac aagcgattta ctttatggaa ggaatagaat gacaaaggtt
    11761 gtttttgaag aaaaatacta tccagctgta aaagaaatgg tttatcgaac tcgtttggcc
    11821 aacggattga cagttgctct tttgcctaaa aaggaattta aagaggttta cgggagtgtc
    11881 actgtacagt ttggttcggt agatacgttt gtcacagaag ttgacggaga tgtaaaacaa
    11941 tatcctggag gaattgctca ttttcttgaa cataaattat ttgagagaga agattctagt
    12001 gatttgatgt cggcttttac gagtctaggt gcagatagta atgcctttac aagctttaca
    12061 aaaacaaact atcttttttc agcaacggat tattttttag aaaatttaga tttacttgat
    12121 gaattggtaa catcagcaca ctttactgaa gcttccattt tgacagagca ggatattatt
    12181 cagcaagaac gagaaatgta ccaagatgat ccagattcgt gtttattctt ttcaacttta
    12241 gcgaatttgt atcctggtac acctttagca actgatatag ttggaagtga ggagtccatt
    12301 tcccaaatca atctaactaa tttgcaagaa aattttacaa agttttacaa acctgtaaac
    12361 atgtctctgt ttttagttgg taattttgat gtggagcgag tacaggacta ttttgaaagc
    12421 aaagaactga aagattcaga ttttcaggaa gtagcaagag aaaagttgtt tttacagcct
    12481 gtaaagccaa cagatagtat gagaatggaa gtatcttctc ccaaactagc gattggagtt
    12541 agaggtaagc gagaagtttc tgaagcggat tgctatcgac atcatatttt attaaaatta
    12601 ttgtttgcaa tgatgtttgg ttggacttcg gatcgttttc aaaaatgtta tgaatcaggt
    12661 aaaattgatg catccttatc tctggaagtt gaagtaacaa gtcgctttca ttttgtcatg
    12721 ttgacaatgg atacgaaaga gccagttgct ttgtctcatc aatttaggaa ggctattcgt
    12781 aattttacaa aggatttaga tattacagag gaacatttag atattatcaa aagagagatg
    12841 tttggcgaat ttttcagtag catgaactct cttgaattta ttgcaacgca atatgatgct
    12901 tttgaaaatg gtgagacaat ttttgatttg ccgaaaattt tacaggaaat tactttagag
    12961 gatgtccttg atgctggaca tcatttaata gatgatggtg acatagttga ttttacaata
    13021 ttcccatcgt agtaacctat tataatagac actagaaaga agggatgaca agtatgagaa
    13081 aaaaaacaat tggagaggtt ttacgattag ctagaatcaa tcagggattg agtttagatg
    13141 aattgcagaa aaagacagaa atccagttag atatgttgga agcaatggaa gcagacgatt
    13201 tcgatcaact tccaagtcct ttttacacgc gttctttctt gaaaaaatat gcatgggctg
    13261 ttgagttaga tgaccaaatt gttttggatg cttatgattc tgggagtatg attacttatg
    13321 aggaagtaga tgttgatgaa gatgagttga caggtcgtag acgttcaagt aagaaaaaga
    13381 agaaaaaaac atcattttta cctttatttt attttatcct ttttgcttta tcgattttaa
    13441 tttttgtgac ttattatgtt tggaactata ttcaaactca accagaggag ccttctcttt
    13501 ctaattacag tgtggttcaa tcaacaagtt caactagctc tgttccccac tcctcaagta
    13561 gtagttcttc tagtatagaa tcagctataa gtgtatcagg cgaaggaaat catgtagaaa
    13621 tcgcttataa gacaagtaag gaaacagtta aattgcaatt ggcagtttca gatgttacaa
    13681 gttgggtcag tgtttcagaa agcgaacttg agggcggtgt aaccttatcg ccaaagaaga
    13741 aaagtgcaga agcaacagtt gcaactaaaa gtcctgtaac aattacgtta ggtgttgtaa
    13801 aaggtgttga tttgacagta gataatcaga ctgttgattt atcgaaatta acagctcaga
    13861 ctggacaaat cactgtaacc tttactaaaa attaaggaaa aacgaatgaa aaaagaacaa
    13921 attcccaatc tcttaacaat aggtcgaatt ctctttatac ctatttttat ctttatttta
    13981 acgataggaa attcgataga gagtcatata gttgcagcta ttatctttgc tgttgccagt
    14041 attaccgact atttagatgg atatttagct cgtaaatgga atgtggtcag taattttggt
    14101 aaatttgcag atcctatggc ggataagtta ctagttatgt cggcttttat tatgttgatt
    14161 gagttaggta tggctccggc ttggattgtt gcagtgatta tctgtcgtga gttagctgtg
    14221 acaggtttaa ggcttttatt ggttgaaact ggtggaacaa ttttagcagc agcaatgcct
    14281 ggaaaaatta aaacttttag tcagatgttt gctattattt tcttgctatt acattggact
    14341 ttgcttggtc aagttctact ttatgtagcc ttatttttca ctatctactc tggctatgac
    14401 tatttcaagg gtagtgccta tgtatttaaa gggacatttg gttcgaaatg aaatcaataa
    14461 ttgatgtaaa aaatctttct tttcgctata aagaaaatca gaactactac gatgtgaagg
    14521 atattacgtt tcacgtgaaa cgtggagaat ggctttcgat tgtagggcat aatggtagtg
    14581 gtaaatcaac tacggttcga ttaattgatg gcttactgga agcagaatcc ggagagattg
    14641 taattgatgg ccaacgtttg actgaggaaa atgtttggaa tatacgtcgt caaatcggta
    14701 tggtttttca aaatccagac aatcaatttg ttggagcgac tgttgaagat gatgttgctt
    14761 ttggtttgga aaatcaggga ctttctcgtc aagaaatgaa aaagagagtg gaagaagctc
    14821 tggctttagt tggcatgttg gactttaaaa agagagagcc agcgcgtcta tcaggtggtc
    14881 aaaagcaacg tgtggccatt gcaggtgttg tagccctaag accagctatt ttaatcttag
    14941 atgaagcaac gagtatgttg gatcctgagg ggcgtagaga acttattggg acagtaaaag
    15001 gaattcgaaa agactatgat atgacggtca tttctattac ccatgatttg gaagaagtcg
    15061 ccatgagtga tcgcgtattg gtcatgaaaa aaggggaaat tgaatcaact agtagtccaa
    15121 gggagctttt ctctcgaaat gatttagatc aaattggatt agacgatcct tttgctaatc
    15181 aattaaaaaa atctttgagc cagaatggct atgatttacc tgaaaattat ttgacagaaa
    15241 gtgagctaga ggataagcta tgggaattgc tctagaaaat gtgaatttta catatcaaga
    15301 aggtactccc ttagcttcag cagctttgtc ggatgtttct ttgacgattg aagatggctc
    15361 ttatacagct ttaattgggc acacaggtag tggtaaatca actattttac aactcttaaa
    15421 tggtttattg gtgccaagtc aagggagtgt gagggttttt gataccttaa tcacctcgac
    15481 ttctaaaaat aaagatattc gtcaaattag aaaacaggtt ggcttggtat ttcagtttgc
    15541 tgaaaatcag atttttgaag aaacggtttt gaaggacgtt gcttttggac cgcaaaattt
    15601 tggagtttct gaagaagatg ctgtgaagac tgcgcgtgag aaactggctc tggttggaat
    15661 tgatgaatca ctttttgatc gtagtccgtt tgagctgtca gggggacaaa tgagacgtgt
    15721 tgccattgca ggcatacttg ccatggagcc agctatatta gtcttagatg agccaacagc
    15781 tggtctagat cctctaggga gaaaagagtt gatgaccctg ttcaaaaaac tccaccagtc
    15841 agggatgacc atcgtcttgg taacgcattt gatggatgat gttgctgaat atgcaaatca
    15901 agtctatgta atggaaaagg gacgtttagt aaaggggggc aaaccaagtg atgtctttca
    15961 agacgttgtt tttatggaag aagttcagtt gggagtacct aaaattacgg ccttttgtaa
    16021 acgattggct gatagaggcg tgtcatttaa acgattaccg attaagatag aggagttcaa
    16081 ggagtcgcta aatggatagt atgattttgg ggcgttatat cccaggggat tcgattgttc
    16141 accgattgga tccacgtagc aaattgcttg ctatgatgct actgattttg attgtgtttt
    16201 gggctaataa ccccttgacg aatctgattc tttttatagc gacagggata tttattgcct
    16261 tgtcaggagt atctctttca ttttttattc agggcttgaa gtctatgttt ttcttgattg
    16321 ccttcacaac tatttttcaa ctatttttca tttctaatgg gaatgtttta tttgagtttt
    16381 cgtttgtgag aatcacggat tatgctttgc aacaagctgg gattattttt tgtcgctttg
    16441 tattgattat tttcttttca actttgttaa ccttaacaac catgccttta agtttggcat
    16501 cagctgtcga agctttatta gcacctttaa agcgtgtgaa agttccagtt catgaaattg
    16561 gattgatgct gtctatgagt ttgcgttttg tcccaacctt gatggatgat acgactcgga
    16621 ttatgaatgc acagaaagct cgtggagtgg attttggaga aggaagcatc gttcaaaaag
    16681 taaaggcgat gattcccatt ttgattcctc tttttgcgac aagtttaaaa cgtgcagatt
    16741 ccttggctat cgctatggaa gcgcgtggct atcagggtgg aaaaggcaga agtcaatacc
    16801 gacaattgaa atggactcta aaggatacgc tgaccattct tgttattctc gtacttggtt
    16861 gttgtttatt tttcttaaaa tcttagtagc tttcaggaat tgataaaaag ttactgtaac
    16921 agtttttgat ataaaggtag ggatatgaac cgttttaaaa aatcaaaata tgtcattatt
    16981 gtttttgtca ctgttctgct tgtgtcagct ctcttagcga cgacttattc aagtacaatt
    17041 gtgacaaaat taggagatgg aatctcattg gttgatagag ttgtacaaaa accttttcag
    17101 tggtttgatt ctgtcaaatc agatttggct catttgacac gaacatataa tgaaaatgaa
    17161 agtttgaaga aacagcttta ccaattagaa gttaaatcaa atgaggtgga aagtttaaag
    17221 acagaaaatg aacaactgcg ccaattgctt gatatgaagt ctaaattgca agccacaaag
    17281 actttagcag cagatgttat tatgcgttct ccggtatctt ggaagcagga gttgacctta
    17341 gatgcaggta gatcaaaagg tgcttctgag aacatgttag ctattgcaaa tggtggcttg
    17401 attgggagtg tttcaaaagt agaggagaac tctactatag tcaaccttct gacaaatacg
    17461 gaaaatgctg ataagatttc tgttaaaatt caacatggct ctactacaat ttatggaatt
    17521 attattggct atgacaagga aaatgacgtt cttaaaatta gccaattaaa tagtaatagc
    17581 gatattagtg cgggagataa ggtgactact ggtggattag gaaactttaa cgttgctgat
    17641 attcctgttg gtgaagtggt tgccacaacg catagtacag actatttgac acgagaagta
    17701 actgttaaat tgagtgcaga tactcataat gtagatgtga tagaattagt ggggaattca
    17761 taatgagaca gttgaagcga gttggagtat ttttattgct tcctttcttt gttctaattg
    17821 acgcccatat tagccagctt ctgggctcat ttttccccca tgtacatttg gctagtcatt
    17881 ttctttttct atttctctta tttgagacga tagaagtatc agagtatctc tacctagtct
    17941 attgttttgt tataggcttg gtttatgatg tttacttttt ccatctaata gggattacaa
    18001 ctctcttatt tatcttattg ggagccttcc ttcataaatt gaatagtgtt attttgttga
    18061 atcgttggac aagaatgcta gctatgattg tgctgacatt cctgtttgaa atgggtagtt
    18121 atcttttggc ttttatggta gggttgacag tagatagcat gtcgattttt atagtctata
    18181 gcttggtacc gacgatgatt ttaaattttt tatggattac tgtttttcaa tttatttttg
    18241 aaaaatatta tctataagaa cgacatataa atgtaacaaa ggcgtaatat ttattaggcc
    18301 tttttttggt atactagtat tgtctttaaa agaaggagta tctacgtaat atgaagaaaa
    18361 aaatcttagc gtcactttta ttaagtacag taatggtttc tcaagtagct gttttaacaa
    18421 ctgcgcatgc agaaacgact gatgacaaaa ttgctgctca agataataaa attagtaact
    18481 taacagcaca acaacaagaa gcccaaaaac aagttgacca aattcaggag caagtatcag
    18541 ctattcaagc tgagcagtct aacttgcaag ctgaaaatga tagattacaa gcagaatcta
    18601 agaaactcga gggtgagatt acagaacttt ctaaaaacat tgtttctcgt aaccaatcgt
    18661 tggaaaaaca agctcgtagt gctcaaacaa atggagccgt aactagctat atcaatacca
    18721 ttgtaaactc aaaatcaatt acagaagcta tttcacgtgt tgctgcaatg agtgaaatcg
    18781 tatctgcaaa caacaaaatg ttagaacaac aaaaggcaga taaaaaagct atttctgaaa
    18841 aacaagtagc aaataatgat gctatcaata ctgtaattgc taatcaacaa aaattggctg
    18901 atgatgctca agcattgact acgaaacagg cagaactaaa agctgctgaa ttaagtcttg
    18961 ctgctgagaa agcgacagct gaaggggaaa aagcaagtct attagagcaa aaagcagcag
    19021 ctgaggcaga ggctcgtgca gctgcggtag cagaagcagc ttataaagaa aaacgagcta
    19081 gccaacaaca atcagtactt gcttcagcaa acactaactt aacagctcaa gtgcaagcag
    19141 tatctgaatc tgcagcagca cctgtccgtg caaaagttcg tccaacatac agtacaaacg
    19201 cttcaagtta tccaattgga gaatgtacat ggggagtaaa aacattggca ccttgggctg
    19261 gagactactg gggtaatgga gcacagtggg ctacaagtgc agcagcagca ggtttccgta
    19321 caggttcaac acctcaagtt ggagcaattg catgttggaa tgatggtgga tatggtcacg
    19381 tagcggttgt tacagctgtt gaatcaacaa cacgtatcca agtatcagaa tcaaattatg
    19441 caggtaatcg tacaattgga aatcaccgtg gatggttcaa tccaacaaca acttctgaag
    19501 gttttgttac atatatttat gcagattaat ttacagaggg actcgaatag agccctcttt
    19561 tcaggtttta ccgtgacaat ccctattaaa aattatatca aaatagcttg aaaatattgg
    19621 aaaagtatgg tagaatgaaa attgtcgtgt gaacgataat actcattctt gatgaattgt
    19681 gaagcagttg cccttgggtc gttttgcgag ttgaagtcaa gaagaggaaa aaaacaaaaa
    19741 ggagaaatac tcatggcagt aatttcaatg aaacaacttc ttgaggctgg tgtacacttt
    19801 ggtcaccaaa ctcgtcgctg gaatcctaag atggctaagt acatctttac tgaacgtaac
    19861 ggaatccacg ttatcgactt gcaacaaact gtaaaatacg ctgaccaagc atacgacttc
    19921 atgcgtgatg cagcagctaa cgatgcagtt gtattgttcg ttggtactaa gaaacaagca
    19981 gctgatgcag ttgctgaaga agcagtacgt tcaggtcaat acttcatcaa ccaccgttgg
    20041 ttgggtggaa ctcttacaaa ctggggaaca atccaaaaac gtatcgctcg tttgaaagaa
    20101 attaaacgta tggaagaaga tggaactttc gaagttcttc ctaagaaaga agttgcactt
    20161 cttaacaaac aacgtgcgcg tcttgaaaaa ttcttgggtg gtatcgaaga tatgcctcgt
    20221 atcccagatg tgatgtacgt agttgaccca cataaagagc aaatcgctgt taaagaagct
    20281 aaaaaattgg gaatcccagt tgtagcgatg gttgacacca atactgatcc agatgatatc
    20341 gatgtaatca tcccagctaa cgatgacgct atccgtgctg ttaaattgat cacagctaaa
    20401 ttggctgacg ctattatcga aggacatcaa ggtgaggatg cagtagcagt tgaagcagaa
    20461 tttgcagctt cagaaactca agcagattca attgaagaaa tcgttgaagt tgtagaaggt
    20521 gacaacgctt aatttataca aatagtaatt acctaggagg gcggggctta gcccggctct
    20581 cctattttca aaaaatatag gagaattaaa atggcagaaa ttacagctaa acttgtaaaa
    20641 gagttgcgtg aaaaatctgg tgccggtgtt atggacgcta aaaaagcgct tgtagaaaca
    20701 gacggtgaca tcgaaaaagc gattgaattg cttcgtgaaa aaggtatggc taaggcagct
    20761 aagaaagctg accgtgttgc tgcagaaggt ttgactggtg tttatgttaa cggtaatgtt
    20821 gcagcagtta ttgaagtaaa cgctgaaact gacttcgttg caaaaaacgc tcaattcgtt
    20881 gaattggtaa atactacagc taaagtcatt gctgaaggaa aacctgctaa caatgaagaa
    20941 gctcttgctt tgataatgcc ttcaggtgaa actcttgaag ctgcatacgt atctgcaaca
    21001 gcaactatcg gagagaaaat ctcattccgt cgctttgcat tgattgaaaa aacagacgca
    21061 caacactttg gagcatacca acataacggt ggacgtatcg gtgttatttc agttgttgaa
    21121 gatggagacg aagcacttgc taaacaattg tcaatgcaca tcgcagcgat gaaaccaaca
    21181 gttctttctt acaaagaatt ggatgagcaa ttcgttaaag atgagttggc acaattgaat
    21241 cacgttatcg accaagacaa cgaaagccgt gcaatggtta ataaaccagc tcttccacac
    21301 ttgaagtatg gatcaaaagc tcaattaact gatgatgtta ttgctcaagc tgaagctgac
    21361 atcaaagctg aattggctgc agaaggcaaa ccagaaaaaa tctgggacaa aattattcca
    21421 ggtaaaatgg atcgcttcat gcttgataac actaaagttg accaagctta cacacttctt
    21481 gcacaagttt acatcatgga tgacagcaag acagttgaag cataccttga atcagttaac
    21541 gcttcggtag ttgagtttgc tcgctttgaa gttggtgaag gtatcgagaa agctgcaaac
    21601 gacttcgaag ctgaagttgc agctacaatg gcagcagcct tgaataacta ataatagaaa
    21661 ggaagcaaat tgcttccttt tttgtatagg gcttagattt ttcctgaaat tgttttttcc
    21721 ccagtctcac tcgattagaa taattaaaac aaaaagccct ataataaggg ctaagtgcat
    21781 ttaggagact atacttcaaa ttcatagtaa gcgcgtcata acaaggtatc tatcctaatt
    21841 ccccaacttc cagtatacta gataaaaaat aattatcgga ggaaaggtat tgtactatga
    21901 taattcaact aagtgattta ggtcaagttc accttgtttg tggcaagaca gatatgaggt
    21961 agggaataga ctcattagcc tatgtagtta aaacccactt tgaattggat tctttctccg
    22021 gtcaagtctt tctcttttgt ggtggacgta aagaccgctt taaagccctt tactgggatg
    22081 gtcaaggatt ttggctacta tataaacgct ttgagaacgg aaaactgact tggctaagta
    22141 cagaaaagga tgtcaaagct ctcgcacctg aacaagtaga ttggcttatg aagggctttt
    22201 ctatcactcc aaaaatatag tagattgaaa ctagaatagt acacctctgc ttctaaaaca
    22261 ttgttagaaa tcgatttgat tgtcctgttc ttatttcatt ttactataaa tccatcagaa
    22321 agttgtgatt tctattgaaa tgaggacttt ctttttacac tcatctgctt tcaaaaagca
    22381 ttctagtcca tctccgatta acgatggact ttatcacctc cttctccagt ccttgtatga
    22441 catcttccaa agttcgaaag gctttattct taaatccacg tttacgaatc tctttccaca
    22501 cttgttcaat ggggttcatc tctggtgtgt atggaggaat aaatgcaaag ccaatattag
    22561 tcggaatctt taaggtactt gatttatgcc atatagcatt gtccataacg agtaaaagat
    22621 aatcatctgg ataagcttgt gaaagctcct attcctaaag cccctttagc gcatagcttt
    22681 ggctcagctt ctattatcgc tcacaccatc catcagaagt ttaatctgaa ggtacccaat
    22741 tatcgccaag aagaagattg ggctaagatg ggtttaccaa tcacacgcaa ggaaatctct
    22801 aattggcata tcaagacgag tcaatactat ttggagcccc tctataacct cttgcgagag
    22861 agactattga ctcagccctt acttcatgcg gatgaaactt cttatagggt gctagagagt
    22921 gatagtcagc tgacttacta ttggactttt ttgtctggga aagctgagaa acaagggatt
    22981 acgctttacc atcatgtcct aatcgatttg ttcatatctt atttcaatcc attataaata
    23041 gtgagaaata tctatcctat cttctagaat gtcttccaaa cgtggaaact ctcgtaaaca
    23101 aagaggtttt agaggcttat ttaccatgga ctaaagttgt acaagaaaag tgcaaataag
    23161 aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt caatatactt
    23221 cgttattgga cggttacaat tcataaagtg ctgtagagag ataacgttca ccgttatctg
    23281 gtgctaggac aaggactttt ttacctgtac ctaatttttt ggcaacctcg atggctccgt
    23341 agatagctgc agctgaggaa atccctacaa ggaagccttc ttttccacca atttcacgtc
    23401 cgagtgcaag agcgtcatct gatgttacac gaacgatacc atcataggct ttagtatcaa
    23461 gtgtatcagg aataaatcca gctgagatac cttgaatttt gtgaggacca ggtttttcac
    23521 cagatagaat agcagattca tctgcttcta ctgcaaaaac ttgaatgtta gaattttctg
    23581 atttgagtgc atgagaaaca ccagaaatcg ttccaccggt acctactcca gcaacaaagg
    23641 catctaatcc atctttaccg aaagcagcta gtatctcagc tcctgttgtt ctttcgtgta
    23701 cttctggatt agctggattg tcaaattgaa gaggaaggaa accatcacgt tcagcagcga
    23761 tttcttgagc cttagcaata gcacctttca ttccctcgct accaggagtt aggacgagtt
    23821 cagcaccata agcttggata attttacgtc gttctacact catagtttca ggcataacga
    23881 tgacgacttt atacccttta gcagcaccta cccatgaaag tccaatacca gtgtttccac
    23941 ttgttgcttc aacaatagta gaaccaggtt tcagaatacc atcttgttca gctttttcaa
    24001 tcatgctaag ggcaatacgg tcttttacag atgaaccagg attaaatgct tcaagcttta
    24061 tatagacgtc tgcagcacct tctggcacga tgttgttaag tttaacaatc ggtgtttgac
    24121 cgattaattc agtaatgttg ttataaatag acatatgaat acctctttgt ataagatatc
    24181 tttagtataa cgctatccat accatttgta aaatatagaa atcctatcga agcgatagga
    24241 tttctatata tcatagatca aagtcattaa ttttcaagtg attgcgttca gttaattcta
    24301 gtaaataggt gtgaaggaaa acgatatcag gtttcgtttg aattgcttca ttttctagta
    24361 tattttggta cgcatctttt tattttgctc gctaaagtag ttgtgagaat gtcaacgaaa
    24421 cgctgtatta ttcattgttt acactaagtt tacaggaact tcaacctctc gtaaaccttg
    24481 gtcagttaaa gtgacttttc cattaaaaaa ctccacaagt gcagctttaa tagtttcttt
    24541 ttcttcttta tcaacataaa tcatcgtatc gacttgatct gtaaagtttg tatccagctc
    24601 catgagatca tgttctttaa ggaagttact gtactcttgg tactgagcat aagacatttg
    24661 aatagcaatg ccagcctgtt cttttatttc aataatacca atttctttga cagctaaggc
    24721 gacactgccg gcgtaagcac gaattagtcc tccagcgcct agtttaatac caccaaagta
    24781 gcgtgtcacg accacacaga cattggtgag attgtgattt tctagtaccc caagcatggg
    24841 aacaccagca gtaccactag gctcaccatc atcacttgta cgtttaattt cactacgttc
    24901 tccaataata aaggcagagc agttatgtgt cgctttgtag tgttcttttt tgatggcagt
    24961 aatgaagtca cgagcctctt cttcgctata aacacgcttg gcatggcaga taaagcgaga
    25021 ttttttgatt tcttcttgga cttggccgtc ctctttaatt gtcctaaatt ccatgattta
    25081 attgtactaa aaaataatct aaagtgctag tttttacgaa taaagaagta tgaaagtaaa
    25141 tttagattat ctcggtcgtt tatttactga gaatgaatta acagaagaag aacgtcagtt
    25201 ggcggagaaa cttccagcaa tgagaaagga gaaggggaaa cttttctgtc aacgttgtaa
    25261 tagtactatt ctagaagaat ggtatttgcc catcggtgct tactattgtc gagagtgctt
    25321 gctgatgaag cgagtcagaa gtgatcaaac tttatactat tttccgcagg aggattttcc
    25381 gaagcaagat gttctcaaat ggcgcggcca attaactcct tttcaagaga aggtgtcaga
    25441 gggactgctt caagcagtag acaagcaaaa gccaacctta gttcatgcgg taacaggagc
    25501 tggaaagaca gaaatgattt atcaagtagt ggctaaagtg atcaatgcgg gtggtgcagt
    25561 gtgtttggct agtcctcgca tagatgtttg tttggagctg tacaagcgcc tgcaacagga
    25621 tttttcttgc gggatagctt tgctacatgg agaatcggaa ccttattttc gaacaccact
    25681 agttgttgca acaacccatc agttattgaa gttttatcaa gcttttgatt tgctgatagt
    25741 ggatgaagta gatgcttttc cttatgttga taatcccacg ctttaccacg ctgtcaagaa
    25801 tagtgtaaag gagaatggat tgagaatctt tttaacagcg acttcgacca atgagttaga
    25861 taaaaaggtc cgtttaggag aactaaaaag actgagttta ccgagacggt ttcatggaaa
    25921 tccgttgatt attccaaaac caatttggtt atcggatttt aatcgctact tagacaagaa
    25981 tcgtttgtca ccaaagttaa agtcctatat tgagaagcag agaaagacag cttatccgtt
    26041 actcattttt gcttcagaaa ttaagaaagg ggagcagtta gaagaaatct tacaggagca
    26101 atttccaaat gagaaaattg gctttgtatc ttctgtaaca gaggatcgat tagagcaagt
    26161 acaagctttt cgagatggag aactgacaat acttatcagt acgacaatct tggagcgtgg
    26221 agttaccttc ccttgtgtgg atgttttcgt agtagaggcc aatcatcgtt tgtttaccaa
    26281 gtctagtttg attcagattg gtggacgagt tggacgaagc atggatagac cgacaggaga
    26341 tttgcttttc ttccatgatg ggttaaatgc ttcaatcaag aaggcgatta aggaaattca
    26401 gatgatgaat aaggaggctg gtctatgaag tgcttgttat gtgggcagac tatgaagact
    26461 gttttaactt ttagtagtct cttacttctg aggaatgatg actcttgtct ttgttcagac
    26521 tgtgattcta cttttgaaag aattggggaa gagaactgtc caaattgtat gaaaacagag
    26581 ttgtcaacaa agtgtcaaga ctgtcaactt tggtgtaaag aaggagttga agtcagtcat
    26641 agagcgattt ttacttacaa tcaagctatg aaggattttt tcagtcggta taagtttgat
    26701 ggagacttcc tgttaagaaa agttttcgct tcatttttaa gtgaggagtt gaaaaagtac
    26761 aaagagtatc aatttgttgt aattccccta agtcctgata gatatgctaa cagaggattt
    26821 aatcaggttg agggcttggt agaggcagca ggctttgagt atctggattt attagagaaa
    26881 agagaagaga gagccagttc ttctaaaaat cgttcagagc gcttggggac agaacttcct
    26941 ttctttatta aaagtggagt cactattcct aaaaaaatcc tacttataga tgatatctat
    27001 actacaggag caactataaa tcgtgttaag aaactgttgg aagaagctgg tgctaaggat
    27061 gtaaaaacat tttcccttgt aagatgagga aaaaatttgc aaaaatagaa tgaaagcgtt
    27121 ataataaaat cataaaaaca aaaatgttta gaaagaaggt actcatatga ttaaatatag
    27181 tatccgtggt gaaaacctag aagtaacaga agcaattcgt gattatgtag tttctaaact
    27241 cgaaaagatc gaaaagtact tccaaccaga acaagagttg gatgcccgaa ttaacttaaa
    27301 agtttatcgt gaaaaaacgg ctaaagtgga agtaacgatt ccgcttggat ctattactct
    27361 ccgcgcagaa gatgtatctc aagatatgta tggttcaatt gaccttgtaa ctgataaaat
    27421 tgaacgtcag attcgtaaaa ataaaacaaa aatcgagcgt aaaaataaaa ataaggtagc
    27481 aactggtcaa ttatttacag atgctttggt ggaagattca aatattgtcc agtctaaagt
    27541 tgttcgttca aaacaaattg atttaaaacc aatggatttg gaagaagcaa ttctacaaat
    27601 ggatttattg gggcatgatt tctttatcta tgtggatgtt gaagatcaga caaccaatgt
    27661 gatttatcgt cgtgaggatg gcgaaattgg tttgttagag gttaaagaat cttaatttca
    27721 atatgtgtaa aggtaggttt actgaattgt aaactacctt tttcttataa ttgatagaat
    27781 tactgattac acttttaaaa agtcgctttt tgaggggtat tatggtataa tgggtgccaa
    27841 gaggttttga atgaaaaaat tttatgtaag tccaattttt cctattctag taggattgat
    27901 tgcgtttgga gtcttatcca ctttcattat ttttgttaat aataatctgt tgacggtttt
    27961 aattttgttt ctttttgtag gaggctatgt ttttttattt aagaaactga gagtgcatta
    28021 tacaaggagt gatgtagaac agatacagta tgtaaaccac caagcggaag aaagtttgac
    28081 agctctattg gaacagatgc ctgtaggtgt tatgaaattg aatttatctt ctggagaggt
    28141 tgagtggttt aatccctatg ctgaattgat tttgaccaag gaagatggtg attttgattt
    28201 agaagctgtt caaacgatta tcaaggcttc agtaggaaat ccgtctactt atgccaagct
    28261 tggtgagaag cgttatgctg ttcatatgga tgcttcttcc ggtgttttgt attttgtaga
    28321 tgtatccagg gaacaagcca taacagatga attggtaaca agtcgaccag tgattgggat
    28381 tgtctctgtg gataattatg atgatttgga ggatgcaact tctgagtcag atattagtca
    28441 aatcaatagt tttgtagcta attttatatc agagttttca gaaaaataca tgatgttttc
    28501 tcgtcgggta agtatggatc gattttatct atttactgac tacacggtgc ttgagggctt
    28561 gatgaatgat aaattttctg ttattgatgc tttcagagaa gagtcgaaac agagacagtt
    28621 gcccttgacc ttaagtatgg gattttctta tggcgatgga aatcatgatg agatagggaa
    28681 agttgctttg ctcaatttga acttggctga agtacgtggt ggcgaccagg tggttgttaa
    28741 ggaaaacgac gaaacgaaaa atccagttta ttttggtggt gggtctgctg cttcaatcaa
    28801 gcgtacacgg actcgtacgc gcgctatgat gacagctatt tcagataaga ttcggagtgt
    28861 agatcaggtt tttgtagtcg gtcacaaaaa tttagacatg gatgctttgg gctctgctgt
    28921 aggtatgcag ttgttcgcca gcaatgtgat tgaaaatagc tatgctcttt atgatgaaga
    28981 acaaatgtct ccagatattg aacgagctgt ttcattcata gaaaaagaag gagttacgaa
    29041 gttgttgtct gttaaggatg caatggggat ggtgaccaat cgttctttgt tgattcttgt
    29101 agaccattca aagacagcct taacattatc aaaagaattt tatgatttat ttacccaaat
    29161 cattgttatt gaccaccata gaagggatca ggattttcca gataatgcgg ttattactta
    29221 tatcgaaagt ggtgcaagta gtgccagtga gttggtaacg gaattgattc agttccagaa
    29281 ttctaagaaa aatcgtttga gtcgtatgca agcaagtgtt ttgatggctg gtatgatgtt
    29341 ggatactaaa aatttcacct cgcgagtaac tagtcggaca tttgatgttg ctagctatct
    29401 cagaacgcgc ggaagtgata gtattgctat ccaggaaatc gctgcgacag attttgaaga
    29461 atatcgtgag gtcaatgaac ttattttaca ggggtgtaaa ttaggttcag atgtactaat
    29521 agcagaggct aaggacacga aatgctatga tacagttgtt attagtaagg cagcagatgc
    29581 catgttagcc atgtcaggta ttgaagcgag ttttgttctt gcgaagaata cacaaggatt
    29641 tatctctatc tcagctcgaa gtcgtagtaa actgaatgta caacggatta tggaagagtt
    29701 aggcggtgga ggccacttta atttggcagc agctcaaatt aaagatgtaa ccttgtcaga
    29761 agcaggtgaa aaactgacag aaattgtatt aaatgaaata aaggaaaagg agaaagaaga
    29821 atgaaagtaa tctttttagc agatgttaaa ggaaaaggta aaaaaggcga aattaaggaa
    29881 gtaccaacag ggtatgcgca aaactttctt atcaaaaaga atctagccaa agaagcgact
    29941 gctcaagctg taggtgaact tcgtggtaaa caaaaatcgg aagaaaaagc tcacgctgag
    30001 atgattgcag aaggaaaagc aattaaagca caacttgaag cagaagaaac tgttgtagaa
    30061 tttgttgaaa aagttggtcc agatggtcgt acctttggtt ctattaccaa taagaagatt
    30121 gcagaagaat tgcaaaagca atttggaatt aagattgata aacgtcatat tcaagtacaa
    30181 gctccgattc gagcggttgg tttgattgat gtgccagtga aaatctatca agatatcaca
    30241 agtgtaatca atcttcgtgt gaaagaagga taagtttaca ctttcttgac aagattgtaa
    30301 aagaagggaa gtctgatggc agaagtagaa gagttacgag tacaacctca agatatctta
    30361 gctgagcaat ccgttttagg ggctatcttt attgatgaga gtaaacttgt ttttgtgcga
    30421 gaatacattg agtctcggga cttttttaag tatgcccatc gtttgatttt ccaagccatg
    30481 gtcgatttat ccgatcgtgg tgatgccata gatgcaacaa cggttcgtac tatccttgat
    30541 aatcaaggtg atttacagaa tattggtggc ttgtcttact tggttgagat tgttaattct
    30601 gtgccaactt ctgctaatgc ggagtattat gctaagattg ttgcagaaaa agcaatgcta
    30661 cgtcgtttaa ttgccaagtt gacagagtct gtcaaccaag cttacgaagc gtcacaacca
    30721 gctgatgaaa ttattgctca ggcagaaaaa gggttgattg atgtcagtga aaatgcaaat
    30781 cgaagcgggt ttaagaacat tcgagatgtg ttgaatctca actttggaaa tctggaagct
    30841 cgctcgcaac aaacgaccga tattacaggt attgcgacag gttatcgtga tttggatcat
    30901 atgacaacag gacttcatga ggaggagttg attatcttag cagctcgtcc agcagttggt
    30961 aagacagcat ttgccttgaa tatcgctcag aatattggga ctaagttgga caaaacggtt
    31021 gctatttttt cactcgaaat gggtgcggaa agcttggtag atcgtatgtt agctgcagaa
    31081 ggcttagtgg agtcacattc tatccgtaca gggcaattga cagatgagga gtggcaaaaa
    31141 tatactattg ctcagggtaa tctagctaac gccagtatct atatcgatga tacgccaggt
    31201 attcggatta cagagattcg ttctcgttct cgtaaattgg ctcaagaaac tggaaatctt
    31261 ggtttgattt tgatagacta tttgcaactt atcacgggaa ctggtcgaga aaatcgtcaa
    31321 caagaagttt ctgaaatttc tcgtcagttg aaaatactag ccaaggaatt gaaggttcca
    31381 gtaatcgctc tgagtcagct ttctcgtggt gtagaacaac gtcaggacaa gagaccggtc
    31441 ttgtctgata ttcgtgaatc tgggtctatt gagcaggacg ctgatatcgt agcttttctc
    31501 tatcgcgatg actactatga acgtggtggt gaagaagagg agggtatccc aaataataag
    31561 gtggaagtta ttatcgagaa aaaccgtagt ggagctcgtg gaacagtgga attgattttc
    31621 caaaaagaat acaataaatt ttcaagtatc tcaaagaggg aggcataaga tgtcagatgc
    31681 atttacagat gtagccaaga tgaaaaaaat caaagaagaa atcaaggcac atgagggaca
    31741 agtcgtagaa atgactttgg agaatggtcg taagcgccaa aaaaatagat tgggtaagct
    31801 aattgaagtt tatccatctc tatttattgt ggagtttggg gatgtggaag gagataaaca
    31861 agttaatgtt tacgttgaat cctttactta ctcagatatt cttacagaaa agaatttgat
    31921 tcattatctt gactaaagtg agaaattttc tcactttttc ttttttctcc gaataattta
    31981 ggtgaaggca atcatcgctt tatattattt ttcaaggagg aagaatgaaa attttaccgt
    32041 ttatagcaag agggacaagt tattacttga agatgtcagt taaaaagctt gttccttttt
    32101 tagtagtagg attgatgcta gcagctggtg atagtgtcta tgcctattcc ggaggaaatg
    32161 gatcgattgc gcgtggggat gattatcctg cttattataa aaatgggagc caggagattg
    32221 atcagtggcg catgtattct cgtcagtgta cttcttttgt agcctttcgt ttgagtaatg
    32281 tcaatggttt tgaaattccg gcagcttatg gaaatgcgaa tgaatgggga catcgtgctc
    32341 gtcgggaagg ttatcgtgta gataatacac cgacgattgg ttccattact tggtctactg
    32401 caggaactta tggtcatgtt gcctgggtgt caaatgtaat gggagatcag attgagattg
    32461 aggaatataa ctatggttat acagaatcct ataataaacg agttataaaa gcaaacacga
    32521 tgacaggatt tattcatttt aaagatttgg atggcggcag tgttgggaat agtcaatcct
    32581 cagcttcaac aggcggaact cattatttta agaccaagtc tgctattaaa actgaacccc
    32641 tagctagcgg aactgtgatt gattactatt atcctgggga gaaggttcat tatgatcaga
    32701 tacttgaaaa agacggctat aagtggttga gttatactgc ctataatgga agctatcgtt
    32761 atgttcaatt ggaggctgtg aataaaaatc ctctaggtaa ttctgttctt tcttcaacag
    32821 gtggaactca ttattttaag accaagtctg ctatcaaaac tgaaccccta gttagcggaa
    32881 ctgtgattga ttactattat cctggagaga aggttcatta tgatcagata cttgaaaaag
    32941 acggctacaa gtggttgagt tatactgcct ataatggaag tcgtcgctat atacagctag
    33001 agggagtgac ttcttcacaa aattatcaga atcaatcagg aaatatctct agctatggat
    33061 ccaataatag ttcaactgtc ggttggaaga aaataaatgg tagttggtat catttcaaat
    33121 caaatggttc taaatcaaca ggatggctga aagacggttc tagctggtat tatttgaaat
    33181 tatctggtga aatgcagaca ggatggttaa aggagaatgg ctcgtggtat tatctgggta
    33241 gttcaggggc aatgaaaaca ggctggtacc aggtctctgg taagtggtat tattcttact
    33301 cttcaggcgc cttagctgtt aatacgacgg tggatggcta cagagtaaac agtgatggag
    33361 aacgagtata gaaaattgga gtaggagaaa tttcctgctc ttttctgtaa gcagtttcct
    33421 tgacattttt cttattttgc gctatcatat atacatataa tatatgggag tctgtgtaca
    33481 gtctgagagg aagtgttaaa cttcgaccgc acctgatctg ggtaatgcca gcggagggaa
    33541 cgatacttag tctaattttg caccttttcc atgtatggta aaggtttttc tttttttaaa
    33601 aaggaaaacg agaagaggag gttcttatga aagcaagcat tgccttgcaa gttttacccc
    33661 tagcacaggg gattgatcgg atagctgtta ttgatcaggt cattgcttat ctgcaaactc
    33721 aagaagtgac gatggtagtg acaccatttg aaacggtctt ggaaggggag tttgatgagc
    33781 ttatgcgcat tctaaaagaa gcgctggaag tggcagggca ggaggcagac aatgtctttg
    33841 ccaatgtcaa aataaatgta ggagagattt taagtattga tgagaaactt gagaagtata
    33901 ctgagacgac acattagtct attgggcttt ctcggagtct tgttaatctg gcagttagca
    33961 ggttttctta aacttctccc caagtttatc ctgccgacac ctcttgaaat tctccagccc
    34021 tttgttcgtg acagagaatt tctctggcac catagctggg cgaccttgag agtggcttta
    34081 ctggggctga ttttgggagt tttgattgcc tgtcttatgg ctgtgctcat ggatagtttg
    34141 acttggctca atgacctgat ttaccctatg atggtggtca ttcagaccat tccgaccatt
    34201 gccatagctc ctatcctggt cttgtggcta ggttatggga ttttgcccaa gattgtcttg
    34261 attatcttaa cgacaacctt tcccatcatc gttagtattt tggacggttt taggcattgc
    34321 gacaaggata tgctgacctt gtttagtctg atgcgggcca agccttggca aatcctgtgg
    34381 cattttaaaa tcccagttag cctgccttac ttttatgcag gtctgagggt cagtgtctcc
    34441 tacgccttta tcacaactgt ggtatctgag tggttgggag gttttgaagg tcttggtgtt
    34501 tatatgattc agtctaaaaa attgtttcag tatgatacca tgtttgccat tattattctg
    34561 gtgtcgatta tcagtctttt gggtatgaag ctggtcgata tcagtgaaaa atatgtgatt
    34621 aaatggaaac gttcgtagaa ttagaatgtt tctgaaaaag aaaagaggaa atcaaaatga
    34681 agaaaacatg gaaagtgttt ttaacgcttg taacagctct tgtagctgtt gtgcttgtgg
    34741 cctgtggtca aggaactgct tctaaagaca acaaagaggc agaacttaag aaggttgact
    34801 ttatcctaga ctggacacca aataccaacc acacagggct ttatgttgcc aaggaaaaag
    34861 gttatttcaa agaagctgga gtggatgttg atttgaaatt gccaccagaa gaaagttctt
    34921 ctgacttggt tatcaacgga aaggcaccat ttgcagtgta tttccaagac tacatggcta
    34981 agaaattgga aaaaggagca ggaatcactg ccgttgcagc tattgttgaa cacaatacat
    35041 caggaatcat ctctcgtaaa tctgataatg taagcagtcc aaaagacttg gttggtaaga
    35101 aatatgggac atggaatgac ccaactgaac ttgctatgtt gaaaaccttg gtagaatctc
    35161 aaggtggaga ctttgagaag gttgaaaaag taccaaataa cgactcaaac tcaatcacac
    35221 cgattgccaa tggcgtcttt gatactgctt ggatttacta cggttgggat ggtatccttg
    35281 ctaaatctca aggtgtagat gctaacttca tgtacttgaa agactatgtc aaggagtttg
    35341 actactattc accagttatc atcgcaaaca acgactatct gaaagataac aaagaagaag
    35401 ctcgcaaagt catccaagcc atcaaaaaag gctaccaata tgccatggaa catccagaag
    35461 aagctgcaga tattctcatc aagaatgcac ctgaactcaa ggaaaaacgt gactttgtca
    35521 tcgaatctca aaaatacttg tcaaaagaat acgcaagcga caaggaaaaa tggggtcaat
    35581 ttgacgcagc tcgctggaat gctttctaca aatgggataa agaaaatggt atccttaaag
    35641 aagacttgac agacaaaggc ttcaccaacg aatttgtgaa ataatgacag aaattagact
    35701 agagcacgtc agttatgcct atggtcagga gaggatttta gaggatatca atctacaggt
    35761 gacttcaggc gaagtggttt ccatcctagg cccaagtggt gttggaaaga ccaccctctt
    35821 taatctaatc gctgggattt tagaagttca gtcagggaga attgtccttg atggtgaaga
    35881 aaatcccaag gggcgcgtga gttatatgtt gcaaaaggat ctgctcttgg agcacaagac
    35941 ggtgcttgga aatatcattc tgcccctctt gattcaaaag gtggataagg cagaagctat
    36001 ttcccgagcg gataaaattc ttgcgacctt ccagctgaca gctgtaagag acaagtatcc
    36061 tcatgaactt agcggtggga tgcgccagcg tgtagcctta ctccggacct acctttttgg
    36121 gcacaagctc tttctcttag atgaggcctt tagcgccttg gatgagatga caaagatgga
    36181 actccacgct tggtatcttg agattcacaa acagttgcag ctaacaactc tgatcatcac
    36241 gcatagtatt gaggaggccc tcaatctcag cgaccgcatc tatatcttga aaaatcgccc
    36301 tgggcagatt gtttcagaaa ttaaactaga ttggtctgaa gatgaggaca aggaagtcca
    36361 aaagattgcc tacaaacgtc aaatcttggc agaattaggc ttagaataag tagaaaaata
    36421 gggagttggt gaagattatc ctttaccagc gccctttttc ttttaaaaat gagaaaattt
    36481 cggtataata gtcaaacaag gtcaaggttt aaagagagag gtgggtttgt tatgagattt
    36541 aaaaatacat cggatcatat tgaggcctac atcaaggcga ttttagatca atctggtatc
    36601 gtggagttgc aacggagtca gttggcagat acctttcagg ttgttcctag tcagattaac
    36661 tacgtgatca agacacgctt tacggaaagt agaggctact tggttgaaag taagcgtggt
    36721 ggcggaggct acattcgtat aggacggatt gagttttcta gtcatcatga aatgctccgg
    36781 gatctgcttt actcgattgg tgagcgagtc agtcaagaaa tttatgagga tattctccag
    36841 cttttggttg agcaggaatt gatgaccaag caggagatga atttgctaga atcagtagct
    36901 ttggatcgcg ttttaggaga agaagctcca gttgttcgag caaacatgct acgtcaggtc
    36961 atacaagagg tagatagaaa agggaagtaa gatgaactat tcaaaagcat tgaatgaatg
    37021 tatcgaaagt gcctacatgg ttgctggaca ttttggagct cgttatctag agtcgtggca
    37081 cttgttgatt gccatgtcta atcacagtta tagtgtagca ggggcaactt taaatgatta
    37141 tccgtatgag atggaccgtt tagaagaggt ggctttggaa ctgactgaaa cggactatag
    37201 ccaggatgaa acctttacgg aattgccgtt ctcccgtcgt ttgcaggttc tttttgatga
    37261 agcagagtat gtagcgtcag tggtccatgc taaggtacta gggacagagc acgtcctcta
    37321 tgcgattttg catgatggca atgccttggc gactcgtatc ttggagaggg ctggtttttc
    37381 ttatgaagac aagaaagatc aggtcaagat tgctgccctt cgtcgaaatt tagaagaacg
    37441 tgcaggctgg actcgtgaag atctcaaggc tttacgccaa cgccatcgta cagtagctga
    37501 caagcaaaat tctatggcca atatgatggg catgccgcag actcctagtg gtggtctcga
    37561 ggactatacg catgatttga cagagcaagc gcgttctggc aagttagaac cagtcatcgg
    37621 tcgggacaag gaaatctcac gtatgattca aatcttgagc cgtaagacta agaacaaccc
    37681 tgtcttggtt ggggatgctg gtgtcgggaa aacagctctg gcccttggtc ttgcccagcg
    37741 tattgctagt ggtgacgtgc ctgcggaaat ggctaagatg cgcgtgttag aacttgattt
    37801 gatgaatgtc gttgcaggga cacgcttccg tggtgacttt gaagaacgca tgaataatat
    37861 catcaaggat attgaagaag atggccaagt catcctcttt atcgatgaac tccacaccat
    37921 catgggttct ggtagcggga ttgattcgac tctggatgcg gccaatatct tgaaaccagc
    37981 cttggcgcgt ggaactttga gaacggttgg tgccactact caggaagaat atcaaaaaca
    38041 tatcgaaaaa gatgcggcac tttctcgtcg tttcgctaaa gtgacgattg aagaaccaag
    38101 tgtggcagat agtatgacta ttttacaagg tttgaaggcg acttatgaga aacatcaccg
    38161 tgtacaaatc acagatgaag cggttgaaac agcggttaag atggctcatc gttatttaac
    38221 cagtcgtcac ttgccagact ctgctatcga cctcttggac gaagcagcag caacagtgca
    38281 aaataaggca aagcatgtaa aagcagacga ttcagatttg agtccagctg acaaggccct
    38341 gatggatggc aagtggaaac aggcagccca gctaatcgca aaagaagagg aagtacctgt
    38401 ctacaaagac ttggtgacag agtctgatat tttgaccacc ttgagtcgct tgtcaggaat
    38461 cccagttcaa aagctgactc agactgacgc taagaaatat ctaaatcttg aagcagaact
    38521 ccataaacgg gttatcggtc aagatcaagc tgtttcaagt attagtcgtg cgattcgccg
    38581 taatcagtca ggaattcgca gtcacaagcg tccgatcggt tcctttatgt tcctaggacc
    38641 cacaggtgtc ggtaagaccg agttggccaa ggctttggca gaagttctct ttgatgacga
    38701 atcagccctt atccgctttg atatgagtga gtatatggag aaattcgcag ccagccgtct
    38761 aaatggagct cctccaggct atgtgggtta cgaagaaggt ggggagttga cagagaaggt
    38821 tcgcaataaa ccctattccg ttctcctctt tgatgaggta gagaaggctc acccagatat
    38881 ctttaatgtt ctcttgcagg ttctggatga cggtgtcttg acagatagca agggacgcaa
    38941 ggtcgatttt tcaaatacca ttatcattat gacatcgaac ctaggtgcga ctgcccttcg
    39001 tgatgataag actgttggtt ttggggctaa ggatattcgt tttgaccagg aaaatatgga
    39061 aaaacgcatg tttgaagaac tgaaaaaagc ttatagaccg gaattcatca accgtattga
    39121 tgagaaggtg gtcttccata gcctatctag tgatcatatg caggaagtgg tgaagattat
    39181 ggtcaagcct ttagtggcaa gtttggctga aaaaggcatt gacttgaaat tacaagcttc
    39241 agctctgaaa ttgttagcaa atcaaggata tgacccagag atgggagctc gcccacttcg
    39301 cagaaccctg caaacagaag tggaggacaa gttggcagaa cttcttctca agggagattt
    39361 agtggcaggc agcacactta agattggtgt caaagcaggc cagttaaaat ttgatattgc
    39421 ataaaagaat aaaagtacca gcatctgacc ataagtcaca gtggagtgaa actcaatgaa
    39481 aatcaaagag caaactaggc agctagccgc aggttgctca aaacactgtt ttgaggttgc
    39541 agatagagct gacgtggttt gaagagattt tcgaagagta taagctgcaa gatgaatgat
    39601 tttcttgtat tgacgttgtt gttgacaaaa agtagcggat aaatgaaatc cattctatta
    39661 tcatagatga taggctggta ggaaattttc aaatagcata caggaaatag atgtatggag
    39721 ttctggtagt agaaagggag agagatgaac attttagttg cagatgacga ggaaatgatt
    39781 agagaaggaa ttgcagcatt tctgacagaa gagggttatc atgtcattat ggctaaggat
    39841 ggacaagagg tcttggaaaa atttcaagat ctccctatcc atctcatggt actggattta
    39901 atgatgccta ggaagagtgg ttttgaagtg ttaaaagaaa tcaatcaaaa gcacgatatt
    39961 cctgtcatcg tcttgagtgc tctgggagat gaaattactc agtcacaggt atttgatctc
    40021 tatgctgatg atcatgtgac aaaacctttt tctttggtac tgcttgtcaa gcgtattaag
    40081 gcgcttatca gacgttacta cgtcatagag gatatttggc gatatcagga tgtaacagtg
    40141 gattttacct cttacaaagc acattataaa aatgaagaaa ttgatctcaa accaaaggaa
    40201 ttactggtac taaagtgttt gattcagcat aaaaatcaag ttttaagtag agagcagata
    40261 ttggaagaaa tttcaaaaga tgtagctgat ttaccttgtg atagggtcgt tgatgtctat
    40321 attcgtactc ttcgcaaaaa attagcttta gattgtatcg tgactgtgaa aaatgttggg
    40381 tataagatta gtttatgata aaaaatccta aattattaac caagtccttt ttaagaagtt
    40441 ttgcaattct aggtggtgtt ggtctagtca ttcatatagc tatttatttg acctttcctt
    40501 tttattatat tcaactggag ggggaaaagt ttaatgagag cgcaagagtg tttacggagt
    40561 atttaaagac taagacatct gatgaaattc caagcttact ccagtcttat tcaaagtcct
    40621 tgaccatatc tgctcacctt aaaagagata ttgtagataa gcggctccct cttgtgcatg
    40681 acttggatat taaagatgga aagctatcaa attatatcgt gatgttagat atgtctgtta
    40741 gtacagcaga tggtaaacag gtaacagtgc aatttgttca cggggtggat gtctacaaag
    40801 aagcaaagaa tattttgctt ttgtatctcc catatacatt tttggttaca attgcttttt
    40861 cctttgtttt ttcttatttt tatactaaac gcttgctcaa tcctcttttt tacatttcag
    40921 aagtgactag taaaatgcaa gatttggatg acaatattcg ttttgatgaa agtaggaaag
    40981 atgaagttgg tgaagttgga aaacagatta atggtatgta tgagcacttg ttgaaggtta
    41041 tttatgagtt ggaaagtcgt aatgagcaaa ttgtaaaatt gcaaaatcaa aaggtttcct
    41101 ttgtccgcgg agcatcacat gagttgaaaa cccctttagc cagtcttaga attatcctag
    41161 agaatatgca gcataatatt ggagattaca aagatcatcc aaaatatatt gcaaagagta
    41221 taaataagat tgaccagatg agccacttat tggaagaagt actggagtct tctaaattcc
    41281 aagagtggac agagtgtcgt gagactttga ctgttaagcc agttttagta gatattttat
    41341 cacgttatca agaattagct cattcaatag gtgttacaat tgaaaatcaa ttgacagatg
    41401 ctaccagagt cgtcatgagt cttagggcat tggataaggt tttgacaaac ctgattagta
    41461 atgcaattaa atattcagat aaaaatgggc gcgtaatcat atccgagcaa gatggctatc
    41521 tctctatcaa aaatacatgt gcgcctctaa gtgaccaaga actagaacat ttatttgata
    41581 tattttatca ttctcaaatc gtgacagata aggatgaaag ttccggtttg ggtctttaca
    41641 ttgtgaataa tattttagaa agctatcaaa tggattatag ttttctccct tatgaacacg
    41701 gtatggaatt taagattagc ttgtagacag attagttttt tattaaagtt catatagggt
    41761 taacataagt gtgttattct ttgtgtagat aaaagaaagg atactaatat ggtattagcg
    41821 attattttag taacattctt tattcgattg atttttttaa agcgttcgat agagaatgag
    41881 aaacgaatcc ttagcaatgg cgggaaagaa tttggagttg agaatacaaa acgattaact
    41941 atggctcata ttgtttttta tctctcttgc ttggttgagg caatggtgca caagacaatt
    42001 tttgatggca tgggcatggt tggtttagtc ttgcttattt tttctatgct gatgttgatg
    42061 ttggtgattc acttgttggg agatatttgg acagtgaagc ttatgcttgt caataatcac
    42121 aaatatgtag atcatatctt gtttaggaca gtaaaacacc ctaattactt tttaaatatt
    42181 ttacctgagt tgattggctt gaccttgttg agtcatgcct atatgacttt tgttttagtt
    42241 tttccagttt atgcagttat tttgtatcga cgaatagctg aagaggaaaa gctattacat
    42301 gaagttataa tcccaaatgg aagcataaag agataaatac aaaattcgac tcatatacag
    42361 ttcatattga agtgatatag taaggttaaa gaaaaaatat agaaggaaat aaacatgttt
    42421 gcatcaaaaa gcgaaagaaa agtacattat tcaattcgta aatttagtat tggagtagct
    42481 agtgtagtag ttgccagctt gttcttagga ggagtagtcc atgcagaagg ggttaggagt
    42541 aagaataacc tcacggttac atctagtggg caagatatat cgaagaagta tgctgatgaa
    42601 gtcgagtcgc atctacaaag tatattgaag gatgtcaata aaaatttgaa aaaagttcaa
    42661 catacccaaa atgtcgactt caacaaaaag ttgagcagaa ttaaaacgaa gtatttgtat
    42721 ggattaaaag agaagtcgga agctgagttg acgttaaaaa caaaagaaac aaaagaagag
    42781 ttaaccgcag cttttgagca gtttaaaaaa gatacattga aatcaggaaa aaaggtagca
    42841 gaagctgaga aaaaagccaa agctcaaaaa gaagaagatc gccgtaacta cccaaccaat
    42901 acttacaaaa caatcgaact ggaaattgcg gaagcagaag taggggtcgc caaggcagag
    42961 cttgagcttg agtttgcaca agctcaagtt caaatacctc aagatactga gaaaattaat
    43021 gctgctaaat ctaaagtaga agctgctaaa agtaatgtta aaaaactaga aaaaattaaa
    43081 tcagatattg aaaaaacgta tttgtataaa ttagataact caaccaaaga aacgccaaaa
    43141 cctagagtgc gaagaaattc tccggaaata aaagctaagg gcagagtcaa aaattatgaa
    43201 gaggctaata ttgaactttc taaatatatg actgatttgt ataaattaga taactcaacc
    43261 aaagaaacgc caaaatctag agtgcgaaga aattctccgc aagtaggcga ttcgagagaa
    43321 cttaaggaaa cgatagacaa agcgaaagaa actctgtcta cctatatggt aactcgttta
    43381 acgaagctgg atccatctgt tttttggttt gcagatcttc ttatggatgc taagaaggtt
    43441 gtggaagaat acaagacaaa attagaggat gcttcagata aaaaatcggt agaagacttg
    43501 cgaaaggaag cagaaggaaa aatagagtct cttatcgtga ctcaccaaaa tagagaaaaa
    43561 gaaaaccaac cagcacccca accaggagga caagcaggtg gttcaatggt tgtaccaccg
    43621 gtgacgcaaa cacctccatc aacttcccaa agtccaggac aaaaggcgac cgaagctgaa
    43681 aagaaaaagt tacaagactt gattcgtcaa ttccaagaag ccttgaacaa actagacgat
    43741 gaaacaaaga ctgttccaga tggggctaaa ctcacaggag aagctggaaa agcctataat
    43801 gagactagaa cttatgcgaa agaagttgtt gacaaaagca agaagcttct atcacagaca
    43861 gcagtgacaa tggatgaatt ggcaatgcaa ttaaccaaat tgaacgatgc catgtctaaa
    43921 ttgaaagaag ctaaagcgaa attggtacca gaggttaaac cacagccgga aaacccagag
    43981 ccaaaaccac aaccagaggg tgagaaacca agcgtaccag atattaatca ggagaaagaa
    44041 aaagctaaac ttgctatagc aacatacatg agcaagattt tagatgatat aaagaaacat
    44101 catctgaaga aagaaaaaca tcatcagatt gttgctctta ttaaggacct tgataaactt
    44161 aaaaagcaag cactttctga aattgataat gtaaatacca aagtagaaat tgagaataca
    44221 gtccacaagg tatttgcaga catggatacg gttgttacta aattccaaaa aggcttaatt
    44281 cagaacacac cgcaggttcc agaagcacca aagagcccag aggtaccaaa ggtttcagat
    44341 acaccaaagg ctccggacac accgcaggtt ccagaagcac caaagagccc agaggtacca
    44401 aaggttccag aa
//
LOCUS       DAVLRT010000008        43225 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLRT010000008 DAVLRT010000000
VERSION     DAVLRT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.008"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(70..1320)
                     /gene="ilvA"
                     /locus_tag="VXM85_000410"
     CDS             complement(70..1320)
                     /gene="ilvA"
                     /locus_tag="VXM85_000410"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1345039.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(1370..1726)
                     /locus_tag="VXM85_000411"
     CDS             complement(1370..1726)
                     /locus_tag="VXM85_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1345040.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(1964..2200)
                     /locus_tag="VXM85_000412"
     CDS             complement(1964..2200)
                     /locus_tag="VXM85_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345041.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(2307..3329)
                     /gene="ilvC"
                     /locus_tag="VXM85_000413"
     CDS             complement(2307..3329)
                     /gene="ilvC"
                     /locus_tag="VXM85_000413"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1345042.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVVEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(3395..3871)
                     /gene="ilvN"
                     /locus_tag="VXM85_000414"
     CDS             complement(3395..3871)
                     /gene="ilvN"
                     /locus_tag="VXM85_000414"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1345043.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(3864..5564)
                     /locus_tag="VXM85_000415"
     CDS             complement(3864..5564)
                     /locus_tag="VXM85_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1345044.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(5765..7432)
                     /locus_tag="VXM85_000416"
     CDS             complement(5765..7432)
                     /locus_tag="VXM85_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1345045.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(7435..7800)
                     /locus_tag="VXM85_000417"
     CDS             complement(7435..7800)
                     /locus_tag="VXM85_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1345046.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(7957..8145)
                     /locus_tag="VXM85_000418"
     CDS             complement(7957..8145)
                     /locus_tag="VXM85_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1345047.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(8261..8437)
                     /locus_tag="VXM85_000419"
     CDS             complement(8261..8437)
                     /locus_tag="VXM85_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1345048.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(8548..8745)
                     /locus_tag="VXM85_000420"
     CDS             complement(8548..8745)
                     /locus_tag="VXM85_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1345049.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(9000..10544)
                     /locus_tag="VXM85_000421"
     CDS             complement(9000..10544)
                     /locus_tag="VXM85_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1345050.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLELLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            10757..11059
                     /gene="gatC"
                     /locus_tag="VXM85_000422"
     CDS             10757..11059
                     /gene="gatC"
                     /locus_tag="VXM85_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1345051.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            11059..12525
                     /gene="gatA"
                     /locus_tag="VXM85_000423"
     CDS             11059..12525
                     /gene="gatA"
                     /locus_tag="VXM85_000423"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1345052.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAHVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TIQSAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            12525..13967
                     /gene="gatB"
                     /locus_tag="VXM85_000424"
     CDS             12525..13967
                     /gene="gatB"
                     /locus_tag="VXM85_000424"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1345053.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            14096..14656
                     /gene="efp"
                     /locus_tag="VXM85_000425"
     CDS             14096..14656
                     /gene="efp"
                     /locus_tag="VXM85_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1345054.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            14678..15067
                     /locus_tag="VXM85_000426"
     CDS             14678..15067
                     /locus_tag="VXM85_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1345055.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            15060..15482
                     /gene="nusB"
                     /locus_tag="VXM85_000427"
     CDS             15060..15482
                     /gene="nusB"
                     /locus_tag="VXM85_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1345056.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREYT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQMVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(15532..15768)
                     /locus_tag="VXM85_000428"
     CDS             complement(15532..15768)
                     /locus_tag="VXM85_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1345057.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(15781..16254)
                     /locus_tag="VXM85_000429"
     CDS             complement(15781..16254)
                     /locus_tag="VXM85_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345058.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(16272..16457)
                     /gene="briC"
                     /locus_tag="VXM85_000430"
     CDS             complement(16272..16457)
                     /gene="briC"
                     /locus_tag="VXM85_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1345059.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(16616..17383)
                     /locus_tag="VXM85_000431"
     CDS             complement(16616..17383)
                     /locus_tag="VXM85_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1345060.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(17380..18246)
                     /locus_tag="VXM85_000432"
     CDS             complement(17380..18246)
                     /locus_tag="VXM85_000432"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1345061.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(18283..19650)
                     /gene="accC"
                     /locus_tag="VXM85_000433"
     CDS             complement(18283..19650)
                     /gene="accC"
                     /locus_tag="VXM85_000433"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1345062.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(19662..20084)
                     /gene="fabZ"
                     /locus_tag="VXM85_000434"
     CDS             complement(19662..20084)
                     /gene="fabZ"
                     /locus_tag="VXM85_000434"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1345063.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(20081..20566)
                     /locus_tag="VXM85_000435"
     CDS             complement(20081..20566)
                     /locus_tag="VXM85_000435"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1345064.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPTPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(20569..21804)
                     /gene="fabF"
                     /locus_tag="VXM85_000436"
     CDS             complement(20569..21804)
                     /gene="fabF"
                     /locus_tag="VXM85_000436"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1345065.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(21826..22557)
                     /gene="fabG"
                     /locus_tag="VXM85_000437"
     CDS             complement(21826..22557)
                     /gene="fabG"
                     /locus_tag="VXM85_000437"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1345066.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(22591..23511)
                     /gene="fabD"
                     /locus_tag="VXM85_000438"
     CDS             complement(22591..23511)
                     /gene="fabD"
                     /locus_tag="VXM85_000438"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1345067.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(23504..24478)
                     /gene="fabK"
                     /locus_tag="VXM85_000439"
     CDS             complement(23504..24478)
                     /gene="fabK"
                     /locus_tag="VXM85_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1345068.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(24597..24821)
                     /locus_tag="VXM85_000440"
     CDS             complement(24597..24821)
                     /locus_tag="VXM85_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1345069.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(24881..25855)
                     /locus_tag="VXM85_000441"
     CDS             complement(24881..25855)
                     /locus_tag="VXM85_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1345070.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIHLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(25855..26289)
                     /locus_tag="VXM85_000442"
     CDS             complement(25855..26289)
                     /locus_tag="VXM85_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1345071.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(26383..27168)
                     /locus_tag="VXM85_000443"
     CDS             complement(26383..27168)
                     /locus_tag="VXM85_000443"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1345072.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            27493..28857
                     /locus_tag="VXM85_000444"
     CDS             27493..28857
                     /locus_tag="VXM85_000444"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1345073.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEDNEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREIGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            28860..29231
                     /locus_tag="VXM85_000445"
     CDS             28860..29231
                     /locus_tag="VXM85_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1345074.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            29445..30719
                     /gene="serS"
                     /locus_tag="VXM85_000446"
     CDS             29445..30719
                     /gene="serS"
                     /locus_tag="VXM85_000446"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1345075.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(30740..31642)
                     /locus_tag="VXM85_000447"
     CDS             complement(30740..31642)
                     /locus_tag="VXM85_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1345076.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEMAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKRSS"
     gene            complement(31752..32300)
                     /locus_tag="VXM85_000448"
     CDS             complement(31752..32300)
                     /locus_tag="VXM85_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1345077.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(32518..33834)
                     /locus_tag="VXM85_000449"
     CDS             complement(32518..33834)
                     /locus_tag="VXM85_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1345078.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALTQS
                     AFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLMVL
                     LGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFNK"
     regulatory      complement(33925..34013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(34177..36513)
                     /locus_tag="VXM85_000450"
     CDS             complement(34177..36513)
                     /locus_tag="VXM85_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1345079.1"
                     /translation="MNKKILETLELDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLTSREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKVKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYVEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(36617..37165)
                     /locus_tag="VXM85_000451"
     CDS             complement(36617..37165)
                     /locus_tag="VXM85_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1345080.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(37162..37464)
                     /gene="zapA"
                     /locus_tag="VXM85_000452"
     CDS             complement(37162..37464)
                     /gene="zapA"
                     /locus_tag="VXM85_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1345081.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            37551..38432
                     /locus_tag="VXM85_000453"
     CDS             37551..38432
                     /locus_tag="VXM85_000453"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1345082.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFDYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            38436..39050
                     /gene="lepB"
                     /locus_tag="VXM85_000454"
     CDS             38436..39050
                     /gene="lepB"
                     /locus_tag="VXM85_000454"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1345083.1"
                     /translation="MNSLKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKLLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            39130..39252
                     /locus_tag="VXM85_000455"
     CDS             39130..39252
                     /locus_tag="VXM85_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEV1345084.1"
                     /translation="MKIKEQTRKLAVGCLKQWFEVVDRTDEVSSKYCFEVADGS"
     gene            39329..41695
                     /locus_tag="VXM85_000456"
     CDS             39329..41695
                     /locus_tag="VXM85_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1345085.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGEQKRQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLTNQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGVALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(41743..43026)
                     /locus_tag="VXM85_000457"
     CDS             complement(41743..43026)
                     /locus_tag="VXM85_000457"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1345086.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        12562 a         9937 c         8210 g        12516 t
ORIGIN      
        1 agacagtgtg actatgcttg tcaatagata ggaaatcaaa atgaaggtat gatatttttt
       61 tattagtcct cagacaagca tattataaag cgtttcatta ccatttaagt taatataagc
      121 tggatcaaaa ccttccattc tacggatcaa acctgcataa tcatgcttat ctgctaaagc
      181 gatcccaatt aatactgggc ctgttccctt gctagctcgt ttgatatact caaaacgtgt
      241 gatatcatca tttggcccca ggatatcatt tacaaactca cgcaaagctc ctggacgctg
      301 tgggaaattg accacaaagt aatgtttgat accatcataa atcaaggcac gctcttccat
      361 ttctggcata cggttgatat cattatttcc tccagaaatg atacaacaaa tggttttccc
      421 cttaatatat tcagctaaaa cctctaaaga ggcgatacta gccgctccag caggttctgc
      481 gactatccct tgcttagagt aaaggtcaat caaggtttca gaaatcaatc cctcatcgac
      541 acctaccaaa gttttaatat gttgacgagt tgcttcatag gtcaactgac ctaccttttg
      601 cacagcaatc ccatccgcaa atttatcaat ttccttgagt ttgactggac ctccagcctc
      661 aaaggcagct ttcatggaac gcgctccatt cgcctctact ccgataacct caatctctgg
      721 acttgtttcc ttgatatagg tagaaacccc ggcaatgaga ccgccaccac caacaggaac
      781 caagacagca tcaaaatcaa tcgattcttt tcgagcttct tctaaaatct cataagcaac
      841 tgttccttga cctgcttgaa catgagcatc atcaaaagga tcaataaagg tacgattttc
      901 agagactgta aattcttgag ctgctttggc tgaggcatca aaggtatctc caactagttt
      961 aatagttaca aaatccccac caaaaaagcg aacctgacca atcttttgtt gtggcgtagt
     1021 aatgggcata aagatagtag caggaatttt catttcatta caagtatagg ctactccctg
     1081 cgcatgattt cccgcagaag cgcaaactac cccacgttca cgttcttcct tgctgagctg
     1141 ggaaatggca taataggcac cacgaatttt aaaggagcga acacgctggg cattttcttt
     1201 tttcaaataa atcttagcac catacttctc cgataaataa tgatcgtaat ccagtggagt
     1261 attcacaacc acaccgttca agaccttgtg agccttgatt atatcttttg aacttaacat
     1321 ataaaacctc tttttctata aaaaacagtc actaatgact gttttttagt tattcatctc
     1381 cgaaaatttc agtaacactt tcatggtatt tatgagccat acgaatcgaa aactctttag
     1441 gaagttcata acgatttgtg gaaatccaat catataacca ttctaccaaa ctttcttcac
     1501 tatcattacg atggaagata aaatagttta aaatttcttt atattctaca tcattcaaat
     1561 ccaacgtgtc tacaaattga ctacgaggaa caaaattaga atctaatttt cttagttgat
     1621 ctgtcaattc tcccaattgt aaatccaact gaaaaaatag tttttgcagt tgcctatcag
     1681 tttcatcaca tcctgtcttg atagaaaaca aattggtaat attcatatga tttcaacccc
     1741 taaataagta catctaatat cccacgaata gctgaagcta aagttttcgc agtccagtca
     1801 tttaaacccg attctttcaa tccagatgca ataatttgct ctatttgata taaaccgcca
     1861 tctatcgtct catttattct gcgaagagga ccttcaatat aggataaagc ttctccgaat
     1921 ctaacagctg atttaggtcc taatgattta ccaactatat ttgttaatgt tttttatttc
     1981 tcaaaatcca tttcgctata taacgagcag atttagtcgc accagctctc tcaaacggag
     2041 aatgaatttc aagaccttct tctctagcta tttgtgccaa tttgaagaat tgttcaaagg
     2101 tcatatgcaa cctaccatct tcaaaattag catcatcata aatgttaatt acagcatttt
     2161 catcatacga ccaatctgct ttattttggt ctgctagcac tattaccgac ggtgtaatta
     2221 cattatacaa ctagaaccaa taataacgat gcaatttttc tcataatctt tttcataaaa
     2281 tcatctccag cgctatatat ttctaattag ttatagattt tgaatgcatc atcgtcgttt
     2341 ttaccaacaa atggcattgc tttacgcaat tctgcaccaa ctttttcaat ttcaaggtta
     2401 gctgcttgtt cacggtaagc agtcaatttt ggacgtccag ctttatagtc atttacaaag
     2461 tcatttgcaa atttaccatt ttggatgtct gccaagacag ccttcatatt ttctttaact
     2521 tgttcagtga ttacacgtgg acctgataca tagtcaccgt attcagcagt gtttgaaata
     2581 gattgacgca ttttcttgaa tccaccttcg tagatcaagt caacgatcaa tttcatttcg
     2641 tgaagaactt caaagtaagc caattctgga gcgtaacctg cttctgtcaa gacttcgaaa
     2701 cctgcttcga taagggcagt caaaccacca caaagtacag cttgttcacc aaacaaatct
     2761 tcttcagttt cttctttgta agttgtttca agaagaccta cacgagccgc tccaacacct
     2821 ttacaccagt ccatagcaat gtttttagca tttcctgttg catcttggta tactgcataa
     2881 agagctggaa caccaaatcc ttcttcgtaa gtacgacgta ccaagtgtcc cggtccttta
     2941 ggagcacaca tgaagacatc tacatccgca ggaactttga taaattcaaa gtggatgttg
     3001 aaaccatggg caaatccaac tgcgtttcca gcttccaagt ttggagcgat ttctgcttcg
     3061 tacaattctt gttgaatttc gtctggcgcc aagatcatga taacatcagc caacttagta
     3121 gcttctacta ctgtgtaagt atcaaatcca tcttcttttg ctttatcaaa agatttacct
     3181 ggacgtacac cgataataac gtcacgacct gaatcacgca agttttgagc atgcgcatgt
     3241 ccttgtgaac cataaccgat aacggcgatt tttttaccgt caagtgctgc tactttaaca
     3301 tctttttcat attccatttg aactgtcata gcttttctct cttttctatt atttattgcc
     3361 ttttaggctg gtttaataaa tttaagttgg atttttaatc gcgggtaaat ccagttgcac
     3421 ccgttcgagc aatattgcga ataccgtatg ggcgaatgac tcgcaatagg gcttcgctct
     3481 tttctgcatt tcccgtcatc tgaatggtaa tcgagcttgg cgctacgtct accactgttg
     3541 cacggaaagg ttgaataatc gctaaaatct cagctctctt ctcagctggc gctgacatct
     3601 taaccaaaat cacctcgcgc tccaaatgag gcttgtctgt aatatctcga atgcgaatca
     3661 catcaatctg acgattgagc tgtttgatga tttgctccac ttcatcatga gaagcaacat
     3721 caataataat agtgatacgc gatacattcg gatcttctgt tgctccaaca gagatgcttt
     3781 caatattaac ctgacgacga gataggacac ctgtaaagcg attgaggact cctgaacgat
     3841 tttgtagttt tgctgttaac attctacgca tggaactgca cccccaacat ctcatgatta
     3901 ctcttaccag ccggtaccat tggtaacacc tgttccttac gagaaatatc tacctcaatt
     3961 agcataggaa catcctcagt gatgacttca aggtcttgag ccaaggtctc aggattgtca
     4021 aacttatagt ttttaatacc ataagcctgc gccatcaatt ggaaatcagg aagggtatca
     4081 aagaccgact ctgatgttct gccttcatag aaggattcct gccactggcg aaccattcca
     4141 agtgaatgat tgttcagcat aaccaccttg attggcacct tgtaaatatt caaaatagcc
     4201 aactcctggt tggtcatttg gaaaccacca tccccaacaa acaagactac ttccttatct
     4261 gggttagcaa ttttagcacc gattgctgct ggaattccaa agcccattgt tcccaaacca
     4321 cctgaagtca ctaactgacg ctcattttgg tagggataat actgagctgt ccacatttgg
     4381 tgttgaccaa cgtctgttac cacaatggca tctccattcg tcaattcacc aattcgttca
     4441 ataactgctt gcggttgaac cacacgctct ttcttgtcat aagaacgaac acgattcttg
     4501 tctttagtga ctttctcaat ccacttttca gtattgttgt gaactgttgg ttctgctagc
     4561 aacatttgca aggccttctt agcatctcca actacaggaa tgtctgcact gataatcttg
     4621 ccaatctcag ctgggtcaat atcaatgtgg gcaaccttag cattcttagc gaaagtctta
     4681 ggattccccg tcaaacggtc atcgaaacga gaaccaatac taatcataaa gtccgcttcc
     4741 gtcatggcaa tatttgctgc gaatgacccg tgcatgcctc ccattccaag aaagagtggg
     4801 tgactcgttg caatcgttcc ttgtcccaaa agactggtta ccactggaat ttgatagcgt
     4861 tctgcaaatt catttagttc cgtagcagcc tcagcataac taattccacc accagctaac
     4921 aagactggct ttttagcctt ggacaattgc ttcaagattt tcttgatttg catatcattc
     4981 ggctcaagag tcggctgata acttggtaaa ttcacttctg gtgaataaat gaagtctgtt
     5041 tctaaagcag atatgtcttt tggtaggtca attacaactg gccctggacg gcctgtagtt
     5101 gcgatatgga cagcttccgt aatgatacgc ggaatatcag ctgtctcacg aacttggtaa
     5161 ttgtacttag tgattggcat ggtaattcct acgatgtctg cctcctgaaa ggcatccttc
     5221 ccaatccctg ctcgcgccac ctgacctgta aagaccaaaa ggggaacgct atcgctcata
     5281 gcatccgcaa tccctgtaat ggcatttgtt gctcctggtc cactagtgac gacggcaacg
     5341 cccaactttc cagttgattt ggcataacct tcagcttcat gcaaacaacc ttgctcatgg
     5401 cgccctagaa tgtggcgaat gcctttaaaa ttatatatcg catcataaaa aggcaaaacc
     5461 gcaccaccag gataaccaaa gatggtatca actcctaaat cacgaagtgt ttccaaaact
     5521 aggtccgacc ccgtcttagg agattctaaa ctgattttct ccattgttcc cctttctttc
     5581 ctcttaaaaa taacttgtta ctatcatacc attttttcaa aatttttcaa gataaaagaa
     5641 gaaattttct gaattttcta ttttaacgtt tatttatgaa tgttattttt gtttttatta
     5701 aaaagatacc gatttcattt actaaaaaag aaaaaagaac tgatttctca gtccttcatt
     5761 aatcttattc cacactaaat aggtatgggt aaacaggttg ttgaccttgg tgaatctcga
     5821 cttcaacgtc ttcgaattct tctacgattt cttgagcgat ttcattggca agttcttcgc
     5881 ttccgtcttc accgacatag aaggttacga tttcactatc ttcatccaac atatgtttca
     5941 aggtttcagt caatgtttgg tgcatatcag ggtttgacac aagaattttt ccatccacca
     6001 tacctagatt atcgttttca tggatttcta agccatcgat cgttgtatca cgcacggctg
     6061 ttgtgacgct tccgctaacg acatcgctaa gagcagctgt catacgctct tggttttctt
     6121 caatggactt gcttggatca aaggcaagaa gacttgtcat accttgagga agagtgcgag
     6181 cctctaccac tactgctggt tgctccaaaa cttctgccgc agattgagct gccatgaaga
     6241 tgttcttgtt gtttggcaag aagatgatgt tacgggcatt gacctgttca acagccttga
     6301 taaagtcttc tgttgaaggg ttcatggttt gaccgccttc gataacataa tccacgcctt
     6361 gagaacagaa gatatctgct agacctttac cagccaccac agcaatcaaa gcatactctt
     6421 tttcttcagc cgacttgata acttgagtag cttctttctc aacctgtgct tcgtgttggt
     6481 tacgcatatt gtcaactttt accttgacca agctaccata tttgagacct tcttgcataa
     6541 caagtcctgg atcttctgta tgaacatgga ctttgacaat ttcatcatcg ttaacaacga
     6601 ggagagaatc cccaagctca tccaagtagt tacggaattc atcgtagtca aaatctttag
     6661 cataggttgg accttgctta agagctacca tgatttcagt acagtaacca aacgtgatgt
     6721 cctcagtcgc tacgtgacca gctacagact tatgatgctc tacattgatc atctcactca
     6781 tgttggcagg agtcgctaca aagtcctcag atgcaatata ttcgccagta agggctgaaa
     6841 ggaaaccttc gtagatgaag accaatcctt gaccacctga gtccacaacg ccaacttctt
     6901 tcaatactgg aagcatgtct ggtgttttag ctagagctgt tttagcacct tccaaggctg
     6961 cgcgcatgac ttcaacagtg tcatctgttt gctcagcttt tttcttagca ccgatagcag
     7021 ctccacgaga aactgtcaaa atcgttcctt caacaggttt catcactgcc ttataggcaa
     7081 cttccacacc tgattggaag gccagagcca agtcttgacc tgttaactcg tctttatcct
     7141 tgatagcttg ggaaaatcca cggaaaagct gagacgtaat cactcctgag ttcccacgcg
     7201 cacccatcaa aagccctttg gcaagaatgc tcgctacttc tccaactgta gaagctggct
     7261 tgtctgcaac ttctttagca ccattttcaa tggtcattcc catatttgtc ccagtatctc
     7321 catctggaac tggaaagacg tttaatgaat tgacatattc agcttgctta ttcaagcgag
     7381 ttgatgcagc ctgcaccatt tcttgaaata agctagtagt aatttttgac acggttattc
     7441 tcctacaact ttgatatttt gaatgtagac atttacagtc tgagcagtaa ttccaagctg
     7501 gttttccaag ctaaaggcaa cacgctcttg aatgtttttt gacacttcac taatctttgt
     7561 tccgtagctt aacacggtat atacatcaac tgcaatactg ccatcttcgg ctgcctttac
     7621 gacgacacct ttagaataat tttccttacc tagcagggct tggaaattat ctttgagggc
     7681 atttttacta gccataccga ccacaccaaa aatctcagtt gctgctccac ctacgacggt
     7741 tgcaatcact tcatctgtta gttcgatttg accatctttt gtattaattt ttacagtcat
     7801 cctttttacc tcaactagtt gatactctat tttatcatat ttcagcccaa gtgtaaaagc
     7861 agaatgatgt atcagcggat atttactcta tttttcaaac gatgttatac ccacaataaa
     7921 agaaaaaaga ccctaaggtc tcctttgctt ttattattaa acgcgttcaa ctttacctga
     7981 tttcaaagca cgagctgaag cccaaacttt tttaggttta ccatcgataa gaacagtaac
     8041 tttttgaagg tttggtttta cggcacgttt tgtttggttc atcgcgtgtg aacggttgtt
     8101 tcctgataca gtcttacgac ctgtaaagta acatacttta gccattgtgt tttcctccta
     8161 ttagatctaa tatagcggat gtgctagcac cacataccgt actatgttat cacactttct
     8221 tgttttttgc aagggaattg gaagattttt tatttgtgtc ttaaatcagg tcttgcgtga
     8281 cattttctgc tctccacatg ccatcgttga ttaacagaac accagaatta aaattatgtg
     8341 tataaaaatc atctctaact gcagctaagg gtatagccgt caagtccaaa tcccacagct
     8401 catctatcga ttttcttaca acaatatctg aatccaaata cagtacacga gactcgctta
     8461 catactttgg aataaaatac ctaaaaaagc cgcatatgaa agtccctcaa aggggagacg
     8521 ataacctttc agaatactac tgtcaatcta aacattcaca atctcactat tcaaagtctc
     8581 tagtcttttt tccattaatt ggaaccattc tcgcggaagg tcatcattaa aaacataaaa
     8641 cttaagatta taatgatgaa cacaaagaga ttttattgtt gtttcaactt tatccatata
     8701 agcattatct gcacctaaga caatcgcttt tttctcttct ttcacttttt atctcatttc
     8761 tttttattcc catcatatgt ttctacgtaa ccattatttt cgcctattcg ttcgtaaaac
     8821 cataccagtg gagattttag atgaagtccc attacggttt acaattttta cattacgaca
     8881 cagagtttta caaatcgatt tcatttgcca aacgtagtta gtaaggcagt tagctagttc
     8941 gccaaatagc gactagcgtc caacaatttg gaactttagt tccaattgtt ggtactgagt
     9001 cacatcttct cctctaactc tacgtctgga tacttgtccg caaaccagcg gagggcaaag
     9061 tcattttcaa agagaaagac tggttggtca aaacggtctt tggctaagat attgcgactt
     9121 gacgacatcc gttcatccaa gtcctcaggc ttgatccaac gaacggtctt tttacccatt
     9181 gggctcatga ccacttccgc attgtattcg ccttccatac ggtgtttaaa gacttcaaac
     9241 tggagttgac caacagctcc cagcatgtac tcacctgttt ggtaattctt ataaagctga
     9301 acggctcctt cttgcaccaa ttgctcaatc cccttgtgga aggatttttg cttcataaca
     9361 ttcttagcag aaactttcat gaaaatttca ggagtaaagg ttggcaatgg ttcaaattca
     9421 aacttgtttt ttccaaccgt caaggtatcc ccaacctgat aagtaccggt atcgtaaacc
     9481 ccgataatat cacctgccac ggcattggtc acattctcac gactctccgc cataaactgg
     9541 gtaacattag atagtttagc ccccttacca gtacgaggga gattgacact catgccgcgc
     9601 tcaaattcgc ctgatacgat acggacaaag gcaatacggt cacggtgacg aggatccatg
     9661 ttggcttgga ttttaaagac aaagcctgag aaatccttgt cataaggatc cacaatttca
     9721 ccatctgttt tcttgtgacc atgtggttct ggagcaaact tgaggaagat ttcaaggaag
     9781 gtctgcacac caaagtttgt cagggctgaa ccgaaaaaga caggcgtcaa ttctccagcc
     9841 agaatagctt cctctgaaaa ctcattccca gcttcattta aaagctcaat gtcatccttg
     9901 acttgctcgt agaaaggatt gctaccaaaa agtttgtctc catcttctag gctagcaaaa
     9961 cgctcatccc ctttataaag ttctaaacgt tggttataga ggtcatacaa gccctcaaag
    10021 gctttcccca tcccgatagg ccagttcata gggtagctag caatgcccaa gatttcttcc
    10081 aattcttgca agagttccaa aggctcacga ccgtcacggt ccagcttgtt cataaaggta
    10141 aagactggaa tgccacgatg tttcacaacc tcaaacaatt tcttggtttg agcctcgatc
    10201 cccttggcag agtccacgac catgaccgca gcatccaccg ccatcaaggt acgataggta
    10261 tcttctgaga agtcctcgtg ccctggcgtg tctaagatat tcacgcgctt gccgtcgtag
    10321 tcaaattgca taacagatga agtaacagaa atcccacgtt gcttctcgat atccatccag
    10381 tcagatttag caaaagtccc tgttttcttc ccttttaccg taccagcctc acgaatctca
    10441 cccccaaagt agagtaactg ctcagtgatg gttgttttcc ccgcgtccgg gtgggagata
    10501 atggcaaagg tacgacgttt cttaatttct tcttgaatat tcataagttc tctttctttg
    10561 attctctatt tttcttgttt caatagctga gaatgatttt tacattggat tttaccattc
    10621 ctttcaatac tccattatat cggattttag catttttttc aatttctatt tcttttcact
    10681 tccccctccc ttatttatag gaaaatatgg taaaatagaa cagactaaaa atcatcattt
    10741 cacgaaagga tgcaagatga aaattacgca agaagaggta acacacgttg ccaatctttc
    10801 aaaattaaga ttctctgaag aagaaactgc tgcctttgcg accaccttgt ctaagattgt
    10861 tgacatggtt gaattgctgg gcgaagttga cacaactggt gtcgcaccta ctacgactat
    10921 ggctgaccgc aagactgtac tccgccctga tgtggccgaa gaaggaatag accgtgatcg
    10981 cttgtttaaa aacgtacctg aaaaagacaa ctactatatc aaggtgccag ctatcctaga
    11041 caatggagga gatgcctaat gacttttaac aataaaacta ttgaagagtt gcacaatctc
    11101 cttgtctcta aggaaatttc tgcaacagaa ttgacccaag caacacttga aaatatcaag
    11161 tctcgtgagg aagccctcaa ttcatttgtc accatcgctg aggagcaagc tcttgttcaa
    11221 gctaaagcca ttgatgaagc tggaattgat gctgacaatg tcctttcagg aattccactt
    11281 gctgttaagg ataacatctc tacagacggt attctcacaa ctgctgcctc aaaaatgctc
    11341 tacaactatg agccaatctt tgatgcgaca gctgttgcca atgcaaaaac caagggcatg
    11401 attgtcgttg gaaagaccaa catggacgaa tttgctatgg gtggttcagg tgaaacttca
    11461 cactacggag caactaaaaa cgcttgggac cacagcaagg ttcctggtgg gtcatcaagt
    11521 ggttctgccg cagctgtagc ctcaggacaa gttcgcttgt cacttggttc tgatactggt
    11581 ggttccatcc gccaacctgc tgccttcaac ggaatcgttg gtctcaaacc aacctacgga
    11641 acagtttcac gtttcggtct cattgccttt ggcagctcat tagaccagat tggacctttt
    11701 gctcctactg ttaaggaaaa tgccctcttg ctcaacgcta ttgccagcga agatgctaaa
    11761 gactctactt ctgctcatgt ccgcatcgcc gactttactt caaaaatcgg ccaagacatc
    11821 aagggtatga aaatcgcttt gcctaaggaa tacctcggtg aaggaattaa cccagaggtt
    11881 aaggaaacca ttcaaagtgc cgctaaacac tttgaaaaac ttggtgctat tgtcgaagaa
    11941 gtcagccttc ctcactctaa atacggagtt gccgtatact acatcatcgc ttcatcagaa
    12001 gcttcatcaa acttgcaacg ctttgacggt atccgttacg gctatcgcgc agaagatgca
    12061 accaaccttg atgaaatcta tgtaaacagc cgaagccaag gctttggtga agaggtaaaa
    12121 cgtcgtatca tgctgggtac tttcagtctt tcatcaggtt actatgatgc ctactacaaa
    12181 aaggctggtc aagtccgtac cctcatcatt caagatttcg aaaaagtctt cgcggattac
    12241 gatttgattt tgggtccaac tgctccaagt gttgcctatg acttggattc tctcaaccat
    12301 gacccagttg ccatgtactt agccgacctc ttgaccatac ctgtaaactt ggcaggactg
    12361 cctggaattt cgattcctgc tggattctct caaggtctac ctgtcggact ccaattgatt
    12421 ggtcccaagt actctgagga aaccatttac caagctgctg ctgcttttga agcaacaaca
    12481 gactaccaca aacaacaacc cgtgattttt ggaggtgaca actaatgaac tttgaaacag
    12541 tcatcggact tgaagtccac gtagagctca acaccaattc aaaaatcttc tcacctactt
    12601 ctgcccactt tggaaatgac caaaatgcca acactaacgt gattgactgg tctttcccag
    12661 gagttctacc agttctcaat aaaggggttg ttgatgccgg tatcaaggct gctcttgccc
    12721 tcaacatgga cctccacaaa aaaatgcact ttgaccgcaa gaactacttc taccctgata
    12781 atcccaaagc ctaccaaatt tctcagtttg atgagccaat cggttataac ggctggattg
    12841 aagtcgagct agaagacggt acgaccaaga aaattggtat cgaacgcgcc cacttagagg
    12901 aagacgctgg taaaaacacc cacggtacag acggctactc ttacgttgac cttaaccgcc
    12961 aaggggtacc tttgattgag attgtatctg aagccgatat gcgttcgcca gaggaggcct
    13021 atgcttatct gacagccctc aaggaagtta tccagtacgc tggcatttct gacgttaaga
    13081 tggaagaagg ttctatgcgt gtggatgcca acatctccct tcgcccttat ggtcaagaaa
    13141 aattcggtac caagactgaa ttgaagaacc tcaactcctt ctcaaacgtt cgtaaaggtc
    13201 ttgaatacga agtccaacgc caggctgaaa ttcttcgctc aggtggtcaa atccgccaag
    13261 aaacacgccg ttacgatgaa gctaacaaaa caaccatcct catgcgtgtc aaggaagggg
    13321 ctgctgacta ccgctacttc ccagaaccag acctacccct ctttgaaatt tctgacgagt
    13381 ggattgagga aatgcggact gagttgccag agtttccaaa agaacgtcgt gcgcgttatg
    13441 tatctgacct tggtttatca gactacgatg ctagtcagtt gactgctaat aaagtcactt
    13501 ctgacttctt tgaaaaagct gttgccctag gtggtgatgc caaacaagtc tctaactggc
    13561 tccaagggga agtcgctcag ttcttgaatg ctgaaggtaa aacactggaa caaatcgaat
    13621 tgacaccaga aaacttggtt gaaatgattg ccatcatcga agacggtact atttcatcta
    13681 agattgccaa gaaagtcttt gtccatctag ctaaaaatgg cggtggcgcg cgtgaatacg
    13741 tggaaaaagc aggtatggtt caaatttcag atccagctat cttgatccca atcatccacc
    13801 aagtctttgc cgataacgaa gctgctgttg ccgacttcaa gtcaggcaaa cgtaacgccg
    13861 acaaggcctt tacaggattc cttatgaagg caaccaaagg ccaagccaac ccacaagttg
    13921 cccttaaact acttgcacag gaattggcga agttgaaaga aaactagaca gaacaaaacc
    13981 agccctaagg ttggtttttt cttctctacc aactcccaat aactattttg gctttatttc
    14041 cagagtattt tatggtaaaa tgaaaagtaa taatatttat taaagaggta aaaacatgat
    14101 tgaagcaagt aaattaaaag ctggtatgac ctttgaaaca gctgacggca aattgattcg
    14161 cgttttggaa gctagtcacc acaagccagg taaaggaaac acgatcatgc gtatgaaatt
    14221 gcgtgatgtc cgtactggtt ctacatttga cacaagctac cgtccagagg aaaaatttga
    14281 acaagctatt atcgagactg tcccagctca atacttgtac aaaatggatg acacagcata
    14341 cttcatgaat acagaaactt acgaccagta cgaaatccct gtagtcaatg ttgaaaacga
    14401 attgctttac atccttgaaa actctgatgt gaaaatccaa ttctacggaa ctgaagtgat
    14461 cggtgtcacc gttcctacta ctgttgagtt gacagttgct gaaactcaac catctatcaa
    14521 aggtgctact gttacaggtt ctggcaaacc agcaacgatg gaaactggac ttgtcgtaaa
    14581 cgttccagac ttcatcgaag caggacaaaa actcgttatc aacactgcag aaggaactta
    14641 cgtttctcgt gcctaatctc tagaaagagg tcattctatg ggaattgaag aacaacttgg
    14701 cgaaatcgtt atcgccccac gtgtacttga aaaaatcatt gctatcgcta ctgcaaaggt
    14761 agagggtgtt cactcttttt caaacagatc agtgtctgat accctttcaa aactttcact
    14821 cggccgtggc atttatctta aaaacgtgga cgaagaactc acagcagata tctatctcta
    14881 ccttgagtac ggagtaaaag ttcctaaggt agcggttgct atccagaaag ctgtcaaaga
    14941 tgccgtccgt aatatggctg atgtagaact cgctgctatc aatattcacg ttgcaggtat
    15001 cgtcccagat aaaacaccaa aaccagaatt gaaagatcta tttgacgagg acttcctcaa
    15061 tgactagtcc actattagaa tctagacgcc aactccgtaa atgcgctttt caagctctca
    15121 tgagccttga gttcggtacg gatgtcgaaa ctgcttgtcg tttcgcctat actcatgatc
    15181 gtgaatatac ggatgtacaa cttccagcct ttttgataga cctcgtttct ggtgttcaag
    15241 ctaaaaagga agaactagat aagcaaatca ctcagcattt aaaagcaggt tggaccattg
    15301 aacgcttaac gctcgtggag agaaacctcc ttcgcttggg agtctttgaa atcacttcat
    15361 ttgacactcc tcagatggtt gctgttaatg aagctatcga gcttgcaaag gacttctccg
    15421 atcaaaaatc tgcccgtttt atcaatggac tgctcagcca gtttgtaaca gaagaacaat
    15481 aaaaagaaaa agtgtttgat agatatcaaa cacttttttc tttgcctccc actatctaaa
    15541 aaaatgataa tagatataat tgtaaacaaa aatccagata ggttttgcat gattgagaaa
    15601 gttaaaaaaa ctatggcaga gaatcgttaa tctcagattg tcggtagaac gataaacaag
    15661 ggcaaaaaag aaaccaatca gactataata taataaacta attggatctc tgtgagatag
    15721 tatcaaatgg ctaatcccaa agatgatagc agataggata acatccaaat agtacttgga
    15781 ctagggaaag aaggtattca taaaataccc tctatcaaga gtctcctcaa aaacaggacc
    15841 gatgattaca ggcaggacaa aagataagat agtcgataaa aaggttggtt gtccatttga
    15901 aaaaagcacg gtaaaatact catcatgaat attcctatga ttaatcaaat gagcatagcg
    15961 tgcccaaaaa ttaccgagaa tctgataaac cacataagtt gcaaataagt agaagacaaa
    16021 tgaccagttc cagctctttt tctcaaagat aaagagcatc tttttctttt ttaacctcca
    16081 aattaataga aggaaacttc ccactaatcc cattgttaaa ataagagaat agacatcagc
    16141 tcctaaccct aaaatgatcg tcacatacaa tccaattgtt tgtggtaaat aggtagatag
    16201 taaaataata agcaaaaata ttccaaattg tcttagtttt tttgtgtttc tcatcgtact
    16261 tttttgaaag attaccctgc tcggaagccg tacttccaag catctatata agaattaagt
    16321 gccccttgcc tcatataggg agcaaattct ctataatata accatctact atatccatct
    16381 tcccaaacag caagaccacc tgaagtttgc tccaagtcct cagttgaaag aactgtaaat
    16441 gtatttgtac ctgtcattgc aagtaccttc ttaaaataga ttgttgtagg ttcacattta
    16501 tagtatattt ctttttttgt ctattttata gcccatctcc tcaactggca atttttcgac
    16561 ctgaattaca tttttccata aaaaatgaga cctttctagt ctcatttagt cattcttagt
    16621 attttctaaa tcgttgatag cgttcttcca gcaactcttc tagcggtttt tgtgaaagtc
    16681 tagccagctc cgtttggagt tcttttttga cactcttaat cagttcttta ctagaaagtc
    16741 ctatttcaga aatcacctta tccaccacgt ccatttctaa cagttcatgc gaagtgattt
    16801 tcatcagttc tgctgcttcc atagcgcgag taccgtcctt ccataaaatg gaagcaaagc
    16861 cttctggact gagaatggca tagatagaat tttccagcat ccagacacgg tccgcgacag
    16921 ctagagccag agccccgcct gaaccacctt caccgataat aatggcgata ataggaactt
    16981 tcaggtcact catttccatg agattgcgag cgatagcttc cccttgacca cgttcttccg
    17041 ctccgacacc aggataagca cctgctgtat tgataaaggt cacaactgga cggccaaatt
    17101 tctcagcctg tttcatcaac cgcagtgcct ttcggtagcc ttctggatgt ggttggccaa
    17161 aattccgttt gaggttgtct tgcaaactct tgcctttttg gataccaacc actgttacag
    17221 cttgatctcc aagccaacca ataccaccaa caactgcacc atcatcacga aaagaacggt
    17281 caccatgtaa ttggataaat tcatcaaaaa tgcctgtcgc aaagtccaag gttgtcaagc
    17341 gactctgctc acgcgcttct ctgactattt ttgcaatatt catctaggac tccctccatg
    17401 caatctgact aggctagcaa tcgtatctgg taagtctctt cttttgacaa tagcatccac
    17461 aaagccatgt tctaatagga attctgcctt ttggaaatcc tcaggcaagc tttcacgaac
    17521 cgtattttca atcacacgac gcccagcaaa accaaccaag ctctgtggtt cagccagaat
    17581 gatatcgcct tccatagcga aagaagctgt cacaccacca gtcgttggat ctgtcaaaat
    17641 ggtcaggtaa aagagaccag catttgaatg gcgtttaacc gccgcagaga tcttagccat
    17701 ctgcatgaga ctcatgattc cttcctgcat acgggctcca ccagaggctg tgaataggac
    17761 aactggcaat ttttcgacag tcgcatactc aaacaaacga gtgatttttt cacctacaac
    17821 cgtacccata gaagccatga taaagttaga atccataatc ccaagagcca cagtctgacc
    17881 tttaataaga gcagttcctg tcacaacggc ttcatgcaga cctgtttttt cacgcataga
    17941 tgccagtttc ttttggtaac cagggaaatg caagggatcc ttgctttcaa tccctgtaaa
    18001 caattctttg aaggttccca tatcaatcgt caaagccaag cgttcttggg cagaaatacg
    18061 aaaggtatag ctacagtgcg gacagatacg ttcacttccc agatccttct gatagatggt
    18121 atgcttacag cctggacact gggaaaataa ttcatctgga acctctggct tagcttgagg
    18181 tttttcccta accgaacgat tgggattgat tcgaatatac ttatcttttt tactaaatag
    18241 agccattgat tccccttttc ggtttaaact cttaaagtca ttttattctt tttcttgata
    18301 tttaggtaag aaggtttcca tcaagaagga agtatcataa tccccagcaa tgacattgcg
    18361 atctgaaatg aggtcaagct ggaaatctgc attggtctgc actccttcaa tctctaattc
    18421 atagagggca cgttgcattt tcatcaaggc gtcaaaacga ttttcgccgt gtactatgat
    18481 tttggcaatc atactatcat aataaggcgg aatggtataa cctggataaa ctgctgaatc
    18541 cacgcgcaag ccaactccac cacttggcag atagagatta gtaatcttac ctggacttgg
    18601 agcaaagtta aaggctgggt tttctgcatt gatacgacac tcgatggcat gaccgcgtag
    18661 gacaatatct tcttgcttaa cagacaaagg ctgacctgcc gcaatgcaaa tctgttcctt
    18721 aacgatatca acacctgaaa caaactctgt tactggatgt tctacctgaa cacgagtatt
    18781 catctccatg aaatagaaat tgctacttgc ttcatcaaga agaaattcaa tggttcctgc
    18841 attctcatag ccaacaaact ctgccgctcg aacagcagca gcacctattt catgacgcag
    18901 cgtttttccg attgcaatcg agggactttc ttccaaaacc ttttggttat tcctttgaag
    18961 agaacaatcc cgctcaccca agtgaatcac atgtccatgc tcatcaccta ggatttgaac
    19021 ctcaatgtgc cgagctggat agataacccg ttctatgtac atggcaccat tgccataatt
    19081 ggccttggcc tcactagagg cagtttcaaa ggcagaaacg aggtcatctg gtttttcaac
    19141 cttacgaatc cctttaccac ctccacctgc tgaagccttg agcataacag gatagccaat
    19201 tttttcagca acaatcaaag cttcttcaga gttatgcact tctccatctg aacctggtat
    19261 aacaggcaca cctgctttaa tcatctgagc acgcgcattg atcttatccc ccatcatatc
    19321 cataacatga ccagatggac cgataaactt gatacctact tcttcacaca tggtcgcaaa
    19381 tttggaattt tcactgagaa atccaaaacc agggtgaata gcttctgcct cagtcaagac
    19441 tgcagctgat agaactgcat taatattgag ataagactct gttgccttgc caggaccaat
    19501 acaaactgct tcatctgcca aaagcgtatg aagagcttcc ttatcagcag ttgaataaac
    19561 cgctaccgtc gcaatcccca attcacgtgc cgcacggata atacgaaccg caatttcacc
    19621 acgattggca attaaaattt ttcgaaacat ggagaacctc cttagttccc aattgcaaaa
    19681 gtaaggatac cactggctgc aagcttgcca tccacttcag cctttgcttc aaccacagct
    19741 atggtgccac gacgttttac aaaagtcgct gtcataacca attggtcgcc tggtacaact
    19801 tgcttcttga acttaacctt gtccatacca gcgtaaaaga ccagttttcc tttattttca
    19861 ggttttgata actccaacac accagcagtt tgcgccaagg cttccataat cagaacacct
    19921 ggcataactg ggtattgagg aaagtggccg ttaaagaaag gttcgttaat ggtcacattt
    19981 ttgatagcaa caatggtatc ctcgctcact tccaagacac ggtccactag aagcatagga
    20041 taacggtggg gaagagcttc tttgattcct tgaatatcga tcatttgata cgtaccaatc
    20101 ctttaccaaa ctcaaccatt tcttcgttag agacgagaat ttccgttacc acaccatcct
    20161 taggagctgg gatttcattc atgactttca tggcttcgat aattaccaat gtttgacctt
    20221 ttttgacact atcaccaact gtaacgaagg caggtttatc tggtccagca gccaagtaaa
    20281 ccactccaac aagtggactc tctacaagat ttccctcact agccacactt gcttcagctg
    20341 gagctggaac ttcttctgct acagtctctg ctggagcaga tgtaggagct actggactcg
    20401 gtgttgctag aacgggtgtt ggagcgactt gagttgcaac ttcaggcaca ggtctcgctt
    20461 cattcttgct aaactgcaac tcatccgtcc catttttata agaaaattct ctcaaacttg
    20521 actggtcaaa ttgagtcatc aagtctttaa tatcgtttaa attcatactt atctattctc
    20581 ccaacgtttg aaagcaagaa ctgcattgtg gcctccaaaa ccaaaagtat ttgaaatagc
    20641 gtatggaatt tctttctcca agccttgtcc ataaacgaca ttagcttcga tataatctga
    20701 tacttcactt gtcccagctg tcattggtac aaagttatga cgcatagctt cgatggtgac
    20761 gatagcttct actgcacccg cagcccccag caaatgtcct gtaaaagact tggttgatga
    20821 tacaggtact tccttaccaa gaacagctac gatagcacca ctttctcctt tttcattggc
    20881 aggagttgac gttccgtgag cattgacata ggctacttgc tctggagaaa tctcagcttc
    20941 ttccaaggct agtttgatgg ccttgatagc tccctgacct tctggatgtg gagaagtcat
    21001 gtggtaggca tcacaagtat ttccgtaacc aaccacttca gccaggatag tagctccacg
    21061 tttttcagcg tgttcaagac tttctagaac caacatccct gaaccttcac ccataacaaa
    21121 cccattgcga tccttatcaa atgggatcga agcacgagtt ggatcctctg tagtagagag
    21181 agctgttaag gcttggaaac cagcgatggc aaaaggtgtg atagaagctt ctgttcctcc
    21241 caccaacatc acatcttgga aaccaaactt aatggagcgg aaggcatccc caatcgcatc
    21301 atttgatgaa gagcaggcag tattgataga tttacaaaca ccgtttgcac caaaacgcat
    21361 ggctacattc ccagaagcca tatttggtaa agcttttgga agagtcattg gtttgacacg
    21421 tttgggtcct ttttcatgaa ggcgaagtac ctgatcttca atttccttga ttccaccaat
    21481 accagatgca acgataacac caaaacgatc cctattaaga gcctctacat caagattggc
    21541 atgatttaca gcctcttggg ctgcatacaa ggcatataaa gaatagttat caaaacggtt
    21601 ggtatctttt tttacaaagt atttatcgaa cggaaaatct tggatttctg ccgcattatg
    21661 cacatcaaag tcactatgat caaattttgt aatgccacca atgccgattt tcccagttgc
    21721 taaactattc caaaattctt ctggtgtatt tccgattgga gatgttactc cataacctgt
    21781 taccactact cgatttagtt tcattctttt cacctctagc tttcgctaca tacttaagcc
    21841 accatcaatg gcaaccactt gaccagttag ataatcttgg cctgctaaaa atactgtcaa
    21901 atctgcaacc tgctctgcct gcccaaattc tttcatcgga atctgagcta gtgtagcttc
    21961 cttaatctta tctgataaga tagctgtcat atcagactca atcattcctg gagcaatcac
    22021 attgactcgt atattccgac tagcgacctc gcgtgccaca gacttggtaa agccaatcaa
    22081 gccagcctta gaagcagcat agttagcttg accaatattc cccatcaaac caacaacact
    22141 agacatatta atgatagcac cttctctggc tttcatcatc ggtttcaaga ctgattgtgt
    22201 catattaaag gcaccagtca gattgacctt gagcactttt tcaaaatctg cttctgtcat
    22261 cttgagcata agagtatctt gggtaatccc tgcattgttg accaaaacat ctactgaacc
    22321 cagttctgca atagcttgat caatcatacg cttagcgtct gcaaaatctg atacatctcc
    22381 tgaaatggga accaccttga taccatagtt tgaaaactca gcgagcaatt cttctgagat
    22441 tgccccacga ctgtttaaga caatgttggc tcctgcttga gcaaacttgt gggcgatggc
    22501 aagaccaatt ccacgactcg aacctgtaat aaagatattt ttatgttcta gtttcatttt
    22561 tttcctttca aaacttctac ttattttagt ctatttttct aaaagtgcta ctaaactcgc
    22621 ttgatcttcc acatgagcta agtgagcagt ttgatcaatt tttttaacaa aacctgacaa
    22681 gactttcccc ggtccaatct cgataaagtt gcttatgcct gcttcttgca tgaccccaat
    22741 actttcatag aaacgaacgg gttccttgac ctgacgcgtc aagagctgag caatgtcctc
    22801 tttttgcatc acagcagctt ctgtattgcc gactagggga caagtaaaat ctgaaaaact
    22861 tacctgagct agagtttcag ctagtttctg gctagcaggt tcaaggagag cggtgtgaaa
    22921 gggacctgac accttaagag gaatcaagcg tttggcacct gcttcttgca aaagttcaac
    22981 cgctcgatca actgcaacca cttctccagc aatgacgatt tgtgcaggtg tgttatagtt
    23041 ggctggagta accactccaa gttcagaagc tttttgacag gcttcttcaa tgacctctac
    23101 tggcgtattg agaactgcta ccatcttgcc agagtcagca ggagccgctt cttccatata
    23161 ggctccacgc ttagctacca aggcaaccgc atcttcaaaa tccaaggcgc cgcttgccac
    23221 caaggcagag tattctccaa gagacaaacc agcaaccata tcaggctgat agcccttttc
    23281 ttgcaataaa cggtagatag caaccgaagt cgctagaatg gctggttgcg tatagcgggt
    23341 ctgattgagt ttgtcttctt ccgtatcgat gagataacgt aaatcataac cgagcacctg
    23401 actcgctcga tcaatcgttt ctttgacaat cggatactga tcatagaaat cccgtcccat
    23461 ccctagatac tgggcacctt gaccagcaaa taaaaaggct gttttagtca tttcttacaa
    23521 ctcctgccca gcgagaggct tcttcttgaa ttttcttagc ggctccgtaa tacaaatctt
    23581 ttaggatttc ttcagctgtt tcttctttag aaacaagccc tgcgatttga cctgccatga
    23641 cagagccacc atccacatca ccgtgaacaa ctgctttggc tagggcacct gctcccattt
    23701 gttcaaagat ttctaaatca ggatcttcct gcttaaaggc atctttttca gccagttcaa
    23761 aatctctagt caactgattt ttaatagcac gaacagcatg accaaagtgc tgagctgaaa
    23821 tcgtagtatc aatatccctt gcttttaaaa ttttctcctt gtagtttgga tgggcattcg
    23881 actcttttgc aactacaaac cgtgtcccca cctgtacagc ctctgcacct agcataaagc
    23941 cagccgcagc accttcacca tccgcaattc ctcctgcagc aataacagga atagatatag
    24001 ctgtggctac ctgtcgcacc aaggtcatgg ttgttaattt accgatatgc cccccagctt
    24061 ccattccttc tgcaataaca gcgtctgcac cgattttttc catgcgttta gctaaagcga
    24121 cactaggaac aacaggaata acgattatcc cagcttcatg gaaacgttcc atatacttgc
    24181 ttggatttcc tgctcctgtt gtgacaactt taacaccttc ttcaataacg agatccacga
    24241 tatcttccac aaagggagat aagagcatga tgttgacccc aaagggttta tcagtcaatg
    24301 atttgatttt atcaatattg gccttgacaa cttctttcgg ggcatttccc ccaccgataa
    24361 ttcctaatcc tccagccttg gaaacagccc ctgccaaatc accatcagca acccaggcca
    24421 tccctccttg gaaaatagga tagtcaatct tcaataattc tgtaatacgc gttttcatag
    24481 tgcctccaac cttccttgct tacgtaatag ttcgatttca ccataatttg acagtcaaac
    24541 tattacctaa acaagaggga gtgggtttct ccctactcct tctactaata ttctgcttat
    24601 tttgcttgct cttcaacgta agcaaccaag tcaccaactg ttttcaagtc attttctgct
    24661 tcgatttgga tatcaaaagc atcttcgatt tctgagatta cttggaacaa gtccaatgaa
    24721 tctgcgtcca aatcatcaaa agttgattca agtgttactt ctgatgcgtc ttttccaagt
    24781 tcttcaacga taatttcttg tactttttca aatactgcca tgataggact cctttaaaat
    24841 aaatagtttt tttataacaa tgtgttcacc acatgattac ctaaattgta agaatgagcg
    24901 tgccccaggt caaacctcca ccgaagcctg atagaagaac agtctggcta ccatctaaat
    24961 ggatgagacc ttgttctaca cactctgaaa gtaaaatcgg gatactggct gcactggtat
    25021 tgccatattc catcatattg gctggaagtt tggctcggtc aacaccaatt tttctagcca
    25081 tcttatccaa aatacggtca ttggcttgat gaagtagcag ataatccaag tctgtcacct
    25141 ctataggaga ttcatcaata gtctgcttga tagacttggc tacatctcga atggcaaaat
    25201 caaagactgt gcgtccatcc atcttcaaaa atgaatctgc actttcttga tctgaaaatg
    25261 gagaatgcaa acctgaatgc ccataagtta aacactcgct gcgacttcca tcgctattga
    25321 gactctcagc taagaaatgc tcttgctcgc tagcttctaa caagacacca ccagcaccat
    25381 ctccaaacaa cacagctgtt gatcgatccg accaatcgac tgccttagag agggtttcac
    25441 taccaatcac caagcctttt tgaaagcgac cagaagcgat aaacttttca gcagttgaaa
    25501 gagcaaatac aaatccactg caagccgcgg ttaagtcaaa agcaaaggct ttattagcac
    25561 caatattagc ttgaacacga gcagctgtag agggcatcat cgaatctgga gtaatggtag
    25621 ctaggatgat aaaatccagt tcttctcctg ttattccagc ttttgccatc agtttcttag
    25681 caacctctgt agccaaatca ctggtagatt ctgttcttga aatatgcctt tgtcgtattc
    25741 ccgttcgact tgaaatccac tcatcattgg tatccataat ctgagccaag tcgtgatttg
    25801 taaccacttg ctctggcaca taatgagcaa cctgacttat ttttgcaaaa gccattattt
    25861 caaatcctcc aaaaattggt aaagattagt caaaccttta cccatgacag caatttcttc
    25921 ctcgctcatg ccatcaataa ttttttctac catggccttg tggaagcgtt tatgcagtct
    25981 atgaatcaag cgacccttct ttgtcaaatg cagatgcacc acacgacgat cctgttctga
    26041 ccgaactcgc tcaatgtagc ccttacgctc taaattgttc aaacttgtcg taacagttcc
    26101 aagagttacc atcaactctt ttgacacttg acttggagtc acatctggag ccttaccgat
    26161 gacatcaatc gtatgcattt ctttgatgga gatatcctta aaacgactac ctctcaagtt
    26221 cacttcctca attacaagga cattgttaaa tatagatgtt aaatattcat tgattcgttg
    26281 gtagtccatt tttcatatcc ctccttcttc aaaaaacttt tacaatcaaa acatttgaca
    26341 gaaagaatta tatcaaaatt caaagaatga tgcaagtatt ttttattttc ctgtaaattt
    26401 aggtcttctt ctctccgaat gagctcgcac tccttctttg aaatcctctg tttgagctaa
    26461 ggatttctgt aggttcagtt ctaaagtagc gtaaccctgc cagtctttaa attggctctc
    26521 ccaaaccaac ttcttaatgg cagcatagga attacttgaa gcacgtctta attttttaag
    26581 aagctgttct ctcgtctttt caagtttttc agcttcagaa acgcggtaaa ccaaccccca
    26641 ttctagggct ttttctgccg ttaaagcctc tcctgtcata gctaattgag cagcacgcgt
    26701 cacaccgata ctgcggctca agagatgaat tccccctgca tctggagcca aaccaacacc
    26761 aacaaaggct tggataaact tagccttatc cgtcgccaaa cagaaatctg ccgcaacggc
    26821 catattcgct gcggcacctg caacagcacc gtcaacttcc attaaaacag gtttggctat
    26881 ttgcttgatt ttataagaaa tcgtattgac caactctgcg atttttgtca atgatggaat
    26941 atcatcctca tccactgccc gcttcatctc taccaaatct cccccaactg agaagacctt
    27001 tccattggca ttgattaaga taaaatgcac agctggattt tcttctgcca aagtcagagc
    27061 ttctaaaatc tcctcacaca tgggaatatg aaaaccattt gcgacctcag gacggttcaa
    27121 ggtaaggatt gccaagtcct cttcaagctg ataaataatg tgttccatca gggctccttt
    27181 ttattttata agcagtttat actcttcgaa aatctcttta aaccgcgtca gcttccatct
    27241 gcaacctcaa aacagtattt tgagctgact tcgtctagct tcttagtttg ctctttgatt
    27301 ttcattgagt gttaaattat ttcactgtca aagtatatct tttttaggag cagaaagcaa
    27361 gaaaaaatcg actgaaagtg tctcagccga taagaaaggc tcgctttagg ctagaaagca
    27421 tcttattttc ttcattgaaa aaagcctttc tttctgctat aatcgtataa aatacttact
    27481 ttaggagttc ttatgaaagt tgttaaattt ggaggtagtt ctcttgcctc tgctagtcaa
    27541 ttagaaaaag ttttaaacat cgtcaaaagc gattcagagc gtcgttttgt agtcgtttct
    27601 gcgcctggta aacgcaatgc tgaagatact aaggttacgg atgccctgat taaatactac
    27661 cgcgactatg ttgcgggtaa cgatattagc aagagccaaa gctggattat cgaccgctat
    27721 gctgctatgg ttagtgaatt gggactaaaa ccagctgtgc tagaaaaaat ttctaaaagc
    27781 attcacgcct tggccactct tcctattgaa gacaatgaat ttctctacga tactttccta
    27841 gcagccggtg aaaataacaa tgccaaattg attgctgcct actttaacca aaatggtatc
    27901 gatgcacgct atatgcaccc tagagaagct gggattgtgg tcacaagtga acctggtcac
    27961 gctcgcatca ttccatcaag ttatgacaag attgaagaat tgacaaacac caatgaagtc
    28021 cttgtcattc ctggtttctt tggtgtcact aaggaaaatc aaatctgtac tttctcacgt
    28081 ggaggttctg atattacagg ttctatcatt gctgctggtg tcaaagccga cctctatgaa
    28141 aactttacgg acgttgatgg tatctttgca gcccaccctg gtattatcca ccaaccacac
    28201 tcgattcctg agttgaccta ccgtgaaatg cgcgagttgg cctatgcagg cttctcagtc
    28261 cttcatgacg aggctcttct tcctgcctac cgtggaaaaa ttcctctggt tatcaagaat
    28321 accaacaacc ctgaccatcc aggtactcgt atcgttctaa aacacagtaa tgatgaattt
    28381 ccagttgtgg gaattgctgg tgactcaggc tttgtcagca ttaatatgtc gaaatacctc
    28441 atgaaccgtg agattggatt tggccgcaag gttctgcaaa tcctggaaga acttaacatc
    28501 ggttgggaac atatgccaac aggtatcgac gatctttcta tcattctccg ttctcgccaa
    28561 ctaactccca tcaaggaaga agaaatcctg cgtcagttgg ttcaaaaggc taaagtagat
    28621 catgcagaaa tcgaacacga cctttctatc attatgattg ttggtgaaaa aatgaagagc
    28681 cacatcggag tgactgctac tgcgacacgc gctctatctg aaaacaaaat caacatccag
    28741 atgatgtctc aaggttctag tgaagtttct attatgtttg ttgtcaataa agaccaagag
    28801 aaagcagcaa ttaaagccct ctacaatgcc ttttttggag aaagtaagga agactaagca
    28861 tggcccaatc acttaacaaa acagtactcc tcagtacgac aggcacttcc tacctctcta
    28921 tcgctgggaa agttgggaaa ttccttgtcg gagatcaggc tttggaattt tacccagatg
    28981 tcaatgtcga acaatttatc cagattcctt ggagccatat caatcagatt ggagccaatg
    29041 tcactggacg caaaatcagc cgccacttcg aagtctttac agacaaagga aaattcctct
    29101 ttgcttcaaa agactcaggt gccattctca aaattgcacg cgaaaaacta ggcaatgaca
    29161 aggtcgtcaa acttccgacc ctgattcaga caattagcca aaaatttaaa aatctatttg
    29221 caaaaaagta gaaactttag tataatcata gcaacggata agtaggttat cttcttcttt
    29281 cagaaagtct gcgggtactg cgagcagata ggagggatag gtgaaattct accgttagta
    29341 acaattacat acacaatcaa gtgcacacct gtgaagttgg atggaaccgt ggccctgcca
    29401 ctccaacgtt ttgtcaggcg tgcttttttc ataaaggagt tcttatgtta gatatcaaac
    29461 gtattcgtac agattttgaa gctgtcgcag aaaaattagc tacacgtggt gtagatgctg
    29521 ctgtcttgaa tgaaatgaaa gaaatcgatg ctaaacgtcg taacatcttg gtcaaggttg
    29581 aaactctcaa agcagaacgt aacacagttt ctgctgagat tgcccaagct aagcgcaaca
    29641 aggaaaatac agatgacaag attgctgcca tgcaaaatct atctgctgag gttaaagcct
    29701 tggatgctga attggcagaa atcgatgcta aattgacaga atttacaacg actcttccaa
    29761 atatcccagc tgacagcgtt cctgttgggg ctgacgaaga cgacaatgtg gaagttcgcc
    29821 gttggggtac tccacgcgag tttgacttcg aacctaaagc tcactgggat ctcggtgaag
    29881 accttggtat tcttgactgg gaacgcggtg gtaaggtaac aggcgctcgc ttcctcttct
    29941 ataaaggcct cggtgctcgt ttggaacgtg ctatctacaa ctttatgttg gatgaacatg
    30001 gaaaagaagg ctatactgaa gtcatcacac cttacatagt caaccatgat tctatgtttg
    30061 gtactggtca gtatccaaaa tttaaggaag atacttttga actcagcgat accaactttg
    30121 tcttgattcc aactgctgaa gttcctctga caaactacta ccgtgatgaa atcttagacg
    30181 gcaaagatct tccaatctac ttcactgcca tgagtccgtc attccgttct gaggctggtt
    30241 ctgccggtcg tgatacgcgt ggcttgatcc gtttgcacca attccacaag gttgaaatgg
    30301 tcaaatttgc caaaccagaa gaatcttacg aagaattgga aaaaatgaca gccaacgctg
    30361 aaaacattct tcaaaaactc aaccttccat accgtgtcgt tgctctctct actggagata
    30421 tgggcttctc agctgcgaag acttacgact tggaagtatg gattccagca caaaacaatt
    30481 accgtgaaat ctcaagctgt tcaaacacag aagatttcca agcccgtcgt gcccaaatcc
    30541 gttaccgtga tgaagcagat ggcaaggtga aactccttca taccttgaac ggttctggac
    30601 ttgcagttgg acgtacagtg gctgcaattc ttgaaaatta ccaaaatgaa gatggttctg
    30661 tgaccatccc agaagcactt cgtccataca tgggtggagc tgaagtcatc aaaccataaa
    30721 aaataaggtt tagctatttc tagctagacc tttttcgtaa ccaaatcaga taagcaccta
    30781 gtacaaagaa taaaatagtt aggcatataa tggtttcagc caataccagg taatccagaa
    30841 atggaagttt caaaattccc tgagccatct tgagcgaggt cgctgtgata atggttggga
    30901 aggtgagggc tgagaaggct ggttgaaaac cttgttttaa aatgttgggc agacgagtta
    30961 aaacaaagaa aaagaaggat tgagaagcca aaatcatgac aatcaagacc caagtcggca
    31021 ggctggttcc tcctactcga actagagaag ccaagagtag agagaaagga gcacagtaga
    31081 ttccttcttg tccaagcaag gctagtggga gtggatgttt ctttaaatcg ctataaataa
    31141 ggggatagag atagaaggtc aagagaaaac caaaactcaa ggtcgcatag gccatttcga
    31201 taatacctac cagaggatag gtcaaggcag ccactgctat ccccacatag agaaccgtcc
    31261 aacttggagt ggcatgaacc ctccgccctg gacaagcaaa cttgatggta aaaccagcaa
    31321 tcaaggtcaa atccaagaga aatgaaaacc accaaatccc ttgtgctacc aaaggaagat
    31381 aagagaatac gcgaaagaca taggtcgata aaatcatccc agccatagga aaggttgcca
    31441 ttcctgacaa aagagggggc ttggtcaatt cttgcttggt ttctttccaa ttaaagagat
    31501 gcagaattag aaagtaaatc cataaaacca aaccagtcag actaaaaaga tgggatagaa
    31561 ccggcaacgt atctaaaata agatttccag ctcctgccaa acctagcaaa caacctgaaa
    31621 atactaaggg gagttttttc atcctaacct ccaataatca tgttagtttc agtataacat
    31681 aaaagcgctt aaatgaggat ttaaaaaaac gaagtccgat tatttcagac ttcattttac
    31741 tcagatatga attaggcata aggttgcaat tctggattaa ttggtgtatt agctaagttg
    31801 ttggcatagt tacagaggat tgctaggctg acaccaaaaa ccacatccaa ggcattttgt
    31861 tgagtgtagc cagcttctaa aaactcagac aaggcttcat ctcctacacg acccttggta
    31921 ttgataactg ccaaggtaaa cttagctagg gtatccaatt taggatctgt ttcaattgga
    31981 gtacgattgc gaagagcttg aatcaagtca tcattcatct ggatttgttt gatggaaaag
    32041 gctgtgtgac ctgcgacaca gaaggcacaa ccattggtca cggctgccgt gatttgcacc
    32101 acttcacgct caacgggtgt caggctgttg cgacggtgga tagatgagac aatttggtag
    32161 gcttctaaaa cagtcggggc attggccaag agaccgatta ggttgggaat atagccattg
    32221 ttgtcttttt ctactgtttc aagaatttct ttcacttctg ctggtgctga ctctactgta
    32281 tggatagtaa atgttgtcat aagatacctc ttttcttatt attgacacta atattattgg
    32341 aaaatcttat aaaatcctga ttcctaagtt tatctaagat aaagctttat tctctcataa
    32401 gattttcgtt gttatattag tttatcacac ttccaatcac ttgcataata tatattatat
    32461 atcaggctga taaaaattat ttataggcaa aaaaatcaca cgagctgtgt gattccatta
    32521 tttgttaaaa tactttttag tttcagcaat aacgactggc gacaagacca agagggcaat
    32581 caagtttggc agagccatca aggcgttaac gatatctgcg ataatccaga ccatatccaa
    32641 ctcgataaat cctcctaaca agaccatgag cacaaaaaac acacggtaga gccagataaa
    32701 gcgagcccca aagaggaact caaaacagcg ttctccgtaa tagttccaac ctagaatcgt
    32761 tgtaaaggca aaaagtacaa ggaagatggt caagagggca ggcccaaagt gtgaaaagac
    32821 tgttgagaaa gctgactgag tcaaggcaac cccattcaag tcaccactcc aaactccagt
    32881 taccaagatg gtcaaaccag ttagagtaca aatgatgagg gtatcaataa aggttcctgt
    32941 catggaaatc aaaccttgct ctactggttc atttgtcttg gcagctgcaa taggagcaga
    33001 acccagacca gattcgtttg agaacacacc acgcgccaca ccattttgaa tagccatccg
    33061 aacgctagca ccagcaaatc cacctaccgc agcaagggga ctaaaagctg aggtaaagac
    33121 taaagcgatt gtgccaggga tttttccgat attaaagaaa ataactgtaa gagttcctaa
    33181 gatataaatg agggccataa aaggaacaac agtagttgaa accttagaaa tagacttgag
    33241 tccaccaaag actgcaatcg ctacaaagac agacaagacg agagctgtga tggctggcga
    33301 aatcgtcgtt gtattttgga tagattctgt aatcgagttg acttgggtga aggttccgat
    33361 tcccaagaga gcaaccaata ctcctgctac tgcaaacaaa acagcaagtg gtcgccactt
    33421 ttctcccatc cctagaagga tatagtgcat gggacctccc gctactgcac catggtcgtc
    33481 cttggtgcgg tatttgatgg ccaagagtcc ttccgcatac ttggtagcca ttccaaagaa
    33541 agccgccatc cacatccaaa atagagctcc tggtccacca accttgatag ccgtcgcaac
    33601 tcctatgata tttcctgttc caacagttga tgccaaggct gtacacagag ctgcaaaact
    33661 ggatacatca ccatgtccct tatcctggat aaaaataagc tgaaaggcct tgggcagacg
    33721 caaaacctgc aagagtccta gccgcatggt taggtaaatc cctgttccga ccaataaaat
    33781 caagaggggc ggtccccaag caaaagcatc gattgattta agcaattcta acatttcctt
    33841 ctcctatcgt ttcaacccca aaagaaagag cacatgcaag atacatgtac tctggaatgc
    33901 ttagataaat gctaaaaagc ggtctatcct agctctgtcc ttttacctga gagtttgagc
    33961 agttgcctgc cttgcccctt cggtgccttt acggtctctc cagagttccg tccatttaca
    34021 gtcatggaaa atcaaacgat tccccacttc tattaaactt cattcggtgt tggtatttaa
    34081 ttgattctaa ttttacaaaa aatgttggct tttgtcaatg tgtttattag taaaaattag
    34141 ttcaacagtt tttactttat aaagtccaga atactgctat cctttaaaag tgacaatagt
    34201 cgcaccactg cctccagcat tttgtggggc atagccgaaa ctcttgacat gtttgtttct
    34261 ttgcaagtat ttggtaactc cttcacggat gactcctgtt ccgataccat ggatgatatc
    34321 aacttgagcc atattgttaa gcaaggcttg gtcgatgaag gtatctagct cattcatggc
    34381 ttctacatag cgcttgcctc gaagatccag tctagcttga ggtcctcgcc cagaagttcg
    34441 tttcacaaca ttgacctgtt tcttcttgac tggtttttct tgctgggctt gaacaagatc
    34501 aaactctttc tcttccaagg tcatcttaat caagccaact tgggcttccc agcgaccgtc
    34561 cttgagttga ctggtcaagg taccacgctg accataactg agaaccacga tatcatctcc
    34621 cacctttgga gctcgttttt tcttggcctt ttgaaggacc ttatttttag acaagtccac
    34681 tttttcagga gccaattttt tcaacttgac cttggcttca atgatttcgt ggggcttgag
    34741 ttgggattta ctgtggagat ttttgagaat ctggtcactt tcacttaagg ccatatccac
    34801 aatctcagca gcctgttcac gcgccttgtt aagctcggtt tccttttcac gattaagctc
    34861 gttgtagagt ttttttagcg cacggttcat cttgagattt tcttgctcca cctcacggat
    34921 attgtccaaa cgtttgcggc tttccagcgt ctgctcttct aattgctcaa tgatacgatt
    34981 gacgtcattg tcctgatcga tctgctgact ggcatctcct acgataactt cagataggcc
    35041 tagacgtttg gcaatttcaa aggcattact tcggccagga acaccctgca taaagcgata
    35101 ggtcgggcga agagttgcag tatcaaactc catactggca ttttgcacaa aggctgtctc
    35161 aataccgtag gccttgagtt ctggatagtg ggtcgtcgcc atggtcttga tttgacgcag
    35221 gcgaaggtcc tccagaatag ccatggcaag ggctgctccc tcttggggat cagtaccagc
    35281 ccccaactca tccaaaagta agagtgaatg ttggttgacc ttgccaagaa tatccacgat
    35341 attggtcata tggctagaga aggtagacaa gctctgctca atagactgct catctccaat
    35401 atcagcaaag atttcttcaa aaataccaac acgacttccc ttgtctgcta aaatcggcaa
    35461 tcctgactgg gccatgacct gtgtcaagcc cagagttttg agcatgatgg tcttcccacc
    35521 tgtattggga cctgtaatga caatagctgt taaatcttga ccaaaataga catcatttgc
    35581 gacggcattt ttgaccaaag gatggcagac atggagcagt tgaatctctt gattttctga
    35641 cagctgaggc acgactgctt gtctttcttg gataaatcga accttggcac gaatcaagtc
    35701 cagatgaccg ataatccaag cgtcattagc aatctcagcc gcatgagggc ggacacgctc
    35761 agaaatttct tggagaatgc gaagcatttc atagcgctca tctgctcgca gactagcaat
    35821 ttcttcgctc agtttgacta cctcacgtgg ttcgatatag acggtgtttc cactagcaga
    35881 aatatcatga acgacacctg caatcttatt gcggtaggtg tttttgactg gtaaaacctg
    35941 acggccattt ctgctagcaa caattccttc cgtcaacatc tgcgcttttt gcttgagcaa
    36001 gtcttgtaaa acatcgcgta cctgactctc gctatcatgt atttttcgac ggattcgcgc
    36061 caattcttca ctggcaaaat tttcaatgaa acccgcatca ttaaaggcct gaagatttcc
    36121 ttgtaattgc ggaaaatcat gtaatttctc aaaccaaagg gctaattctt ccaagctgac
    36181 attttccaga ttggcgtaaa aactttgaag ttctcggctg gtaagaagca cgcgtttcaa
    36241 gagtaggaac tcctcgatat tgagatccgc tcccatctcc aacctcttgc agactcctgc
    36301 aatttcctta gttgagagaa tagtaaaatg cggttgctcg acgaaaagag cctgcatttc
    36361 tttcatctca gcaaaagcct gtttgatttt atctgctttg gcagtcggag ccagctgtct
    36421 caattgctcc aagccctgct cggtcaacaa atgaggctca aacaaggcct tgaccttatc
    36481 gagctctaat gtttctaata ttttcttatt catctttatt tccttgtctt tgaatgtcta
    36541 ggtgtggtag ctttttacat tctgatagat tcgagtcaaa tctgtaaaca aagggctagg
    36601 aaaactcctg ccctttttat ccgattaaat ttgtcaccca gatttgtttg agccaactgg
    36661 ttgttatcgg tatactctgg atgatgtgtt ttgcgacgat actcttttca agaggatttt
    36721 gtataactgc catggggatg gtcgccaaga ttgtcaaggc catttgcaag acaaataagg
    36781 tcaccaacat ggacaagata cctgctgaaa cttggaacaa cttaccaccc agttttttgc
    36841 taggaatcaa gtgtaagaga agaccaagca aacgaccaat gctatagaca atcccaaata
    36901 caagcaagta gccgatacct gcataaaaga ccttatccag ctgaaagagt tgatccgatg
    36961 ggaaaaagaa agtcccctga ccttcctgcg aatttgcata agggagcaat aaatggaatt
    37021 gctctccaag ccccttataa aactggccag ccataaaagc cgatgccatg gctgaaatca
    37081 ggtaataaac ctgtaagagc aggcctctcc gatagccgat ataaaatccc caaaccaaga
    37141 ccaatagaag aaggaatgaa atcataagga atcctcaatc ttgctctgtt cttgcttaca
    37201 agtcacaagc ttgtgacgga gttcttctag ctcttgctcc ttatcgtcaa attcaatctc
    37261 acggctgagc tgagttgata aacagttgac tgccaacaaa agagcgattg tttcatcatc
    37321 tgcgctaggc atttgttctt taattgcttg gtatttttct gtcgcaacct tagcgatttc
    37381 ctccataaaa aggttatcat gttcgcttgt caaggttaac gattttttcc cgaatgtaaa
    37441 tttgaatcga tttagatttg ccataaaatt cacctcacga tattatacca aaattcgcta
    37501 attttgtcag tttttacaaa ttttactgct tttgtggtac aatagaaact atggcaagta
    37561 tcacactcac accaagcgaa aaggatattc aggcttttct tgaacactat caaaccagtc
    37621 tggctcctag caagaatccc tatatccgct actttttgaa actacctcaa gcaacggttt
    37681 ctatctatac ttctggaaaa atcttgcttc agggtgaagg ggctgaaaaa tacgccagtt
    37741 tctttgacta tcaagctgta gagcaaacca gcggacaaaa tcttccttta attgggacag
    37801 atgaggtggg aaatggttcc tactttggtg ggcttgcagt tgtggctgcc tttgtcacac
    37861 ctgaccagca cgacttttta cgaaaactcg gtgtggggga ttctaagact ctgaccgacc
    37921 aaaagatccg tcagattgct cctattctca aggaaaaaat ccagcaccag gcactccttc
    37981 tctcacccag caagtacaac gaggtcatcg gagaccgcta caatgctgtt tcggttaagg
    38041 ttgccctcca taatcaggct atctatctcc tccttcaaaa aggtgttcag cctgagaaaa
    38101 ttgtgattga tgcctttacc agtgctaaaa attatgacaa gtacttggca caagaggcca
    38161 atcgtttcag caatcctatc agcttagaag aaaaggctga gggcaaatac ttggctgtcg
    38221 cagtttcttc tgtcattgcg cgtgatctct ttctggaaaa tcttgaaaat ttgggacgag
    38281 aactgggtta tcagcttcca agtggagctg gaacggcttc tgacaaggtg gctagccaga
    38341 ttttgcaagc ctatggtatg cagggactca acttctgcgc caaattgcac tttaaaaaca
    38401 ctgaaaaagc gaaaaaacgc ttagaaaggt aagttatgaa ttcacttaaa aatttcttaa
    38461 aagagtgggg attgttcctc ctaattctgt cattactagc tttaagtcgt atcttttttt
    38521 ggagcaatgt tcgcgtagaa ggacattcca tggatccgac cctagcggat ggcgaaattc
    38581 tcttcgttgt taagctcctc cctattgacc gttttgatat cgtggtggcc catgaggaag
    38641 atggcaataa ggacattgtc aaacgtgtca tcggaatgcc tggcgatacc attcgttacg
    38701 aaaatgataa actttacatc aatgacaaag aaacggacga gccttatcta gcagactata
    38761 tcaaacgctt caaggatgac aaactccaaa gcacttactc aggcaagggc tttgaaggaa
    38821 ataaaggaac tttctttaga agtatcgctc aaaaagccca agccttcaca gttgatgtca
    38881 actacaacac caactttagc tttactgttc cagaaggaga ataccttctc ctcggagatg
    38941 accgcttggt ttcgagcgac agccgccacg taggtacctt caaagcaaaa gatatcacag
    39001 gggaagctaa attccgcttc tggccaatca cccgtatcgg aacattttaa gagacctaag
    39061 aggccgagaa tcacaaatct cagcctcttc ttctatcgtg agaagtgatt gtttattgac
    39121 aaatactcaa tgaaaatcaa agaacaaact aggaagctag ccgtaggctg cttaaaacag
    39181 tggtttgagg ttgtggatag aactgacgaa gtcagctcaa aatactgttt tgaggttgca
    39241 gatggaagct gacgtggttt gaagatattt tcgaagagta taaaataaaa acaatcccaa
    39301 ctctcaccta ttcatgcaaa ggaattttat ggaagtttat ttttcaggaa ccattgaacg
    39361 gattattttt gaaaatccca gcaattttta tcgcatcctc ctcctagaaa tcgacgatac
    39421 ggacgcagag gattttgatg attttgaaat cattgtcaca ggaaccatgg ctgatgtaat
    39481 tgagggcgaa gactatactt tttgggggca aattgtccag cactccaagt atggagaaca
    39541 actgcaaatc agtcgttatg atcgcgcaaa accaactagt aagggattgg tcaagtactt
    39601 ttcaagtagc catttcaagg ggattggtct caagacagct cagaaaatcg tagataccta
    39661 tggcgaaaat accattgatg aaattttgca acacccagaa aagttagaag gcatcgcagg
    39721 actctctgcc aaaaatcgcg aggctttcgt ctccactctc cgtctcaact acggaacgga
    39781 gatggttttg gccaaactag ccaactacgg cattcccaac aaactagcct ttcagattca
    39841 agacttttac aaggaagaaa cccttgatgt ggttgaaaat tatccctacc agctggttga
    39901 ggatatcaag ggtttgggct ttacaattgc tgaccaacta gctgaggaac taggcatcga
    39961 aagtcaggct cctgaacgct tccgcgccgg tctagttcac agtctttttc aggcctgtat
    40021 ggaaacagga gacacctatg ttgaagcacg ggatttgctg gaacaaaccc ttactctcct
    40081 tgagtcttcc cgtcccgtag aactagaccc cagccaagtt gcccaagagc tctcctacct
    40141 gatcgaagaa gacaaggttc agcagattga taccaagatc tttgacaaca gcctcttttt
    40201 cgctgaggaa ggtatccgca gtcacttgat tcgtatcctt gaaaaaggag aacagaagag
    40261 acaagattta gaaactattc aaaagcatat cactactgtc gagcaagaac tggggattga
    40321 gtatgataac attcaaaaac aagctatttg tgacgctatc cagaacaagg tctttatcct
    40381 gacaggtggg cctggtactg gtaagacaac tgttatcaat ggaatcatcg ctgtttatgc
    40441 ccttttagaa ggacttgact tcaggaagaa aagcaatctg cccattcttc ttgctgctcc
    40501 aactggtcga gctgctcgtc gcatgaatga attgacaggt ttgcctagcg cgaccataca
    40561 tcgccacttg ggaatgacag gtgacgatga taccagtcat ctggaagatt acctagatgc
    40621 tgactttatc atcgtggatg aattctctat ggtagatact tggctgacca atcaactctt
    40681 ctccaatatc tcttctaaca gtaagatcct catcgtaggt gacagtgatc agctaccttc
    40741 tgtcagtcct ggacaggttc tagcggatct gcttcatatt cctttgattc ctcagactcg
    40801 cttggaaaaa atttatcgac aaagcaaaga atcaaccatc gtcaccctag ctagtcagat
    40861 tcgacagggc atcttgccag ctgatttcac ccaaaaaaaa gctgaccgtt cctactttga
    40921 aattgctagt ggccatattc ctgccaccat tgaaaaaatc ttaggtgtcg ccctcagaaa
    40981 tggtattcct gcccgtgata tccaagttct ggctcctatg taccgaggga cggcagggat
    41041 tgatgctatc aatcagctca tgcaagacct gctcaatccc ccacaaaaag atcaactcag
    41101 ttttgaagct cctcagtgcc actatcgtaa gagagacaag gtcattcatt tggttaacga
    41161 tgctgaaatc aatgtcttta atggggattt aggagctatc acagacctga ttcctggtaa
    41221 atacaccgaa tcgaaacaag acgagattgt cattgatttt gatggtaatg aagtctctta
    41281 cccccgtaac gaatggtaca agattcgctt ggcctatgcc atgagtattc ataagtctca
    41341 gggaagtgag ttcccagttg tcatcctacc gattactagt gctagccgac gtatgctgga
    41401 gcgaaatctc atctacacag ccattacacg tgccaaaagc aaactcatct tactaggcga
    41461 attacaagcc ttcgactatg ctacccaaca catcggaact gcccgaaaaa cctatctgat
    41521 tgaacgcttc agtgatctac tggagaatgt tgaagaaaag caacaagctg tctctgaaac
    41581 agtcacatca agtgcctctg aacaatccta catcctaacc gaagaaaact gggacagcat
    41641 tccagccatg attgggatta cagacacaga cctcaaagag atttttggaa aatagtacat
    41701 cagaaaaccc accaattcag gtgggttttc tgtttattaa gattatttta ctgttgctgt
    41761 gcttgtgatc aattcaacag cttttttgat agtgatatca tgtttcaaca tgtcagctga
    41821 aagcaagttt tgaacttgtg caacttccat gttgtagtct gctgccaatt gctcaacttc
    41881 tttttggatt tcttcttctg aagcatcaaa tccttcagct ttggcaactg cttcgataac
    41941 aaggttagtc ttagtacgtg actcagcttc tgcttggtat tggttgtgaa ggtcttcttg
    42001 agtagttcca gtgatttgga agtacatgtc agggttgatc ccttgacgtt gcaagttccc
    42061 aaggaattca tttactgaac ggtgaacttc ttcatggatc atttcttctg gaagttctac
    42121 gatttcagcg ttttctacag ctgtatcaat tgctgcacct tcaactgcat ctttgtaagc
    42181 ttcttcttta gcagcagcca attctttgcg gtatttttct ttcaagtcag caagtgtttc
    42241 aacttcttca tcaatgtctt ttgcaagttc atcgtcaaga gctggaactt ctttagcttt
    42301 tacttcgtgg atagttgtca cgaatttagc ttctttacct gcaaggtctt ctgcttggta
    42361 gtcttctggg aatgttacga taacatcaac ggtttcgcca gctgagtgac ctaccaattg
    42421 gtcttcgaaa ccagggatga attgacctga accaagtcca agtgagaagt tttcaccttt
    42481 tccaccgtca aattcaacac cgtcgataga accaacgaag tcgatcacaa cagtgtcgcc
    42541 gttttcagca gcagcttcct tgataaccaa ttcagccaga ttgttgcgtt cgcgttcgat
    42601 acgctcttca acatcagcgt cagttacttc tttttctaca tcaactgata cttcaaggtt
    42661 tttgtagtca cccaatttta cttcaggttt tgtaacgact tcagcagcga taacccagtc
    42721 ttgacctttt tccattgaag ttacgtcaat ttttggttgg gcaaccactt caagaccagc
    42781 ttcttttaca gctgcttcat aagcgtttgg caaaagagcg ttcataacgt cttggtaaag
    42841 tgattcttca ccaaattttt tgtcgaagat aggacgtgga aggtgacctt tacggaaacc
    42901 tggaacatta agagatttct tcactgactt gaagacacgg tccaattctg gtttgatttg
    42961 gtcttgagag atagtgaaag tcaagacacc acggtttgtt tctttgtttt caaatgatac
    43021 agacattctg tcatttctcc ttaaaatttt ttaaatacag tctattataa cattttctat
    43081 aaataaaatc tagactaatg cagccaatta tagacaacaa gcctttttcc taactaaaac
    43141 actcataaag tatagacaat tttagactac tttggatatc ttttggttag tcattttcca
    43201 aaactaattt atctaatttt tcagt
//
LOCUS       DAVLRT010000009        41372 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLRT010000009 DAVLRT010000000
VERSION     DAVLRT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.009"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            172..1401
                     /gene="arcA"
                     /locus_tag="VXM85_000458"
     CDS             172..1401
                     /gene="arcA"
                     /locus_tag="VXM85_000458"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1345087.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1459..2475
                     /gene="argF"
                     /locus_tag="VXM85_000459"
     CDS             1459..2475
                     /gene="argF"
                     /locus_tag="VXM85_000459"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1345088.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2640..3587
                     /gene="arcC"
                     /locus_tag="VXM85_000460"
     CDS             2640..3587
                     /gene="arcC"
                     /locus_tag="VXM85_000460"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1345089.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3796..5307
                     /locus_tag="VXM85_000461"
     CDS             3796..5307
                     /locus_tag="VXM85_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1345090.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5329..6660
                     /locus_tag="VXM85_000462"
     CDS             5329..6660
                     /locus_tag="VXM85_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1345091.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLTDKE
                     ELVELLTRCAQNYQLRYEEFDYLASLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(7064..7354)
                     /locus_tag="VXM85_000463"
     CDS             complement(7064..7354)
                     /locus_tag="VXM85_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345092.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(7342..7593)
                     /locus_tag="VXM85_000464"
     CDS             complement(7342..7593)
                     /locus_tag="VXM85_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1345093.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            7797..8045
                     /locus_tag="VXM85_000465"
     CDS             7797..8045
                     /locus_tag="VXM85_000465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345094.1"
                     /translation="MKIKEQTRKLATGCSKHCFEVVDKTDEVSSKHCFEVVDRTDEVS
                     SKHCFEVVDRTDEVSSKHCFEVADRTDEVSNIYTARRR"
     gene            complement(8082..8966)
                     /locus_tag="VXM85_000466"
     CDS             complement(8082..8966)
                     /locus_tag="VXM85_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1345095.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(9111..10262)
                     /locus_tag="VXM85_000467"
     CDS             complement(9111..10262)
                     /locus_tag="VXM85_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1345096.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(10408..12174)
                     /locus_tag="VXM85_000468"
     CDS             complement(10408..12174)
                     /locus_tag="VXM85_000468"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1345097.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTEHPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYCACQLLGPLHK"
     gene            complement(12257..15373)
                     /locus_tag="VXM85_000469"
     CDS             complement(12257..15373)
                     /locus_tag="VXM85_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1345098.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRSAGIPLSLA
                     EVEVYTYK"
     gene            complement(15383..17677)
                     /locus_tag="VXM85_000470"
     CDS             complement(15383..17677)
                     /locus_tag="VXM85_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1345099.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(17736..18536)
                     /locus_tag="VXM85_000471"
     CDS             complement(17736..18536)
                     /locus_tag="VXM85_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1345100.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(18533..19306)
                     /locus_tag="VXM85_000472"
     CDS             complement(18533..19306)
                     /locus_tag="VXM85_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1345101.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(19331..19801)
                     /locus_tag="VXM85_000473"
     CDS             complement(19331..19801)
                     /locus_tag="VXM85_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1345102.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(19792..20223)
                     /locus_tag="VXM85_000474"
     CDS             complement(19792..20223)
                     /locus_tag="VXM85_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1345103.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(20216..20656)
                     /locus_tag="VXM85_000475"
     CDS             complement(20216..20656)
                     /locus_tag="VXM85_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1345104.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(20668..21306)
                     /locus_tag="VXM85_000476"
     CDS             complement(20668..21306)
                     /locus_tag="VXM85_000476"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1345105.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            complement(21421..22824)
                     /locus_tag="VXM85_000477"
     CDS             complement(21421..22824)
                     /locus_tag="VXM85_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1345106.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            23000..23773
                     /locus_tag="VXM85_000478"
     CDS             23000..23773
                     /locus_tag="VXM85_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1345107.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(24901..26406)
                     /gene="adcA"
                     /locus_tag="VXM85_000479"
     CDS             complement(24901..26406)
                     /gene="adcA"
                     /locus_tag="VXM85_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1345108.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVTKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYVEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(26416..27222)
                     /locus_tag="VXM85_000480"
     CDS             complement(26416..27222)
                     /locus_tag="VXM85_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1345109.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(27215..27919)
                     /locus_tag="VXM85_000481"
     CDS             complement(27215..27919)
                     /locus_tag="VXM85_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1345110.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVSHA"
     gene            complement(27919..28359)
                     /gene="adcR"
                     /locus_tag="VXM85_000482"
     CDS             complement(27919..28359)
                     /gene="adcR"
                     /locus_tag="VXM85_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1345111.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(28514..29782)
                     /gene="dltD"
                     /locus_tag="VXM85_000483"
     CDS             complement(28514..29782)
                     /gene="dltD"
                     /locus_tag="VXM85_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1345112.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(29775..30014)
                     /gene="dltC"
                     /locus_tag="VXM85_000484"
     CDS             complement(29775..30014)
                     /gene="dltC"
                     /locus_tag="VXM85_000484"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1345113.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(30028..31272)
                     /gene="dltB"
                     /locus_tag="VXM85_000485"
     CDS             complement(30028..31272)
                     /gene="dltB"
                     /locus_tag="VXM85_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1345114.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(31269..32819)
                     /gene="dltA"
                     /locus_tag="VXM85_000486"
     CDS             complement(31269..32819)
                     /gene="dltA"
                     /locus_tag="VXM85_000486"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1345115.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKSDSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     ITDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDSVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(32840..32971)
                     /locus_tag="VXM85_000487"
     CDS             complement(32840..32971)
                     /locus_tag="VXM85_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1345116.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(33202..33906)
                     /locus_tag="VXM85_000488"
     CDS             complement(33202..33906)
                     /locus_tag="VXM85_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1345117.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPISNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(33976..35802)
                     /gene="glpO"
                     /locus_tag="VXM85_000489"
     CDS             complement(33976..35802)
                     /gene="glpO"
                     /locus_tag="VXM85_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1345118.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTSAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            complement(35844..37352)
                     /gene="glpK"
                     /locus_tag="VXM85_000490"
     CDS             complement(35844..37352)
                     /gene="glpK"
                     /locus_tag="VXM85_000490"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1345119.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(37511..38938)
                     /locus_tag="VXM85_000491"
     CDS             complement(37511..38938)
                     /locus_tag="VXM85_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1345120.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSENENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            39103..39975
                     /gene="hslO"
                     /locus_tag="VXM85_000492"
     CDS             39103..39975
                     /gene="hslO"
                     /locus_tag="VXM85_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1345121.1"
                     /translation="MNKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            39962..40942
                     /gene="dusB"
                     /locus_tag="VXM85_000493"
     CDS             39962..40942
                     /gene="dusB"
                     /locus_tag="VXM85_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1345122.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(41172..>41372)
                     /locus_tag="VXM85_000494"
     CDS             complement(41172..>41372)
                     /locus_tag="VXM85_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV1345123.1"
                     /translation="NANGSMATGWLKDGDTWYYLEASGAMKESQWFKVSDKWYYVNGS
                     GALAVNTTVGGYRVNANGKWVN"
BASE COUNT        12396 a         8969 c         7409 g        12598 t
ORIGIN      
        1 tgtattctat cctatgttat gtataataac ataaaattgt gctaaacatc ataatttata
       61 ttattttccg agaaattata gccattgaca ttacctagga attgcgttat aacatacatg
      121 taagcggtac ccaaaatggt gccaagtcaa aatttttaag gaggaaaata catgtcttca
      181 catccaattc aggtcttctc agaaattggg aaactgaaaa aagttatgtt gcaccgtcca
      241 ggcaaggagt tagaaaactt gttgccggac tatcttgaaa ggcttctttt tgatgatatt
      301 cctttcttgg aagatgctca aaaagaacat gatgcatttg cccaagctct tcgcgatgaa
      361 ggaattgagg ttctctacct agaacaactc gctgctgaat cattgacctc tccagaaatc
      421 cgcgatcaat ttatcgagga atacttagac gaagccaaca tccgtgatcg tcaaaccaag
      481 gttgctattc gtgaattgct tcacggcatc aaggacaacc aagaattggt tgaaaaaaca
      541 atggctggga ttcaaaaagt tgaattgcca gaaattcctg acgaagctaa agatctaact
      601 gacttagttg aatcagatta tccatttgca attgacccga tgccaaacct ctatttcact
      661 cgcgacccat ttgcaacaat tggaaacgcc gtatcgctta accacatgtt tgcagacact
      721 cgtaaccgtg aaacactcta cggtaagtat atcttcaaat accacccaat ctatggcgga
      781 aaagtggatt tggtctacaa ccgtgaagaa gatacgcgta tcgaaggtgg agacgagtta
      841 gttctttcta aagacgtcct tgcagtaggt atctctcaac gtacagacgc agcttctatc
      901 gaaaaacttt tggtcaacat cttcaagaaa aatgttggct tcaagaaagt tttggccttt
      961 gaatttgcta acaaccgtaa attcatgcac ttggatactg tcttcactat ggtagactat
     1021 gacaagttca ctattcaccc agaaatcgaa ggcgaccttc acgtttactc agttacttac
     1081 gaaaacgaaa aacttaaaat cgttgaagag aaaggtgact tagctgaact tcttgctcaa
     1141 aaccttggtg tagaaaaagt tcatttgatt cgttgcggtg gtggcaatat cgtagcagct
     1201 gcgcgtgaac aatggaacga cggttctaac actttgacca tcgcacctgg tgtggtagtt
     1261 gtttatgacc gcaataccgt gaccaataag attttggaag aatacgggct tcgcttgatt
     1321 aagattcgcg gaagtgaatt ggttcggggc cgtggtggac ctcgttgtat gtctatgcca
     1381 tttgaacgtg aagaagtgta atcgctgttc ggcattcgtc aatagaaaat gtaaaaaata
     1441 gaaagaggaa ataataaaat gacaaattca gtattccaag gacgcagctt cttagcagaa
     1501 aaagacttta cccgtgcaga gttagaatac cttattggtc tttcagctca cttgaaagat
     1561 ttgaaaaaac gcaatattca acaccactac cttgctggca agaatatcgc tctcctattt
     1621 gaaaaaacat ctactcgtac tcgtgcagcc tttacaactg cggctatcga ccttggtgct
     1681 cacccagaat acctcggagc aaatgatatt cagttgggta aaaaagaatc tactgaagat
     1741 actgctaaag tattgggacg tatgtttgac gggattgaat tccgcggatt cagccaacgt
     1801 atggttgaag aattggcaga attctcaggc gttccagtat ggaacggtct aactgacgaa
     1861 tggcacccaa ctcaaatgct cgctgactac ttgactgttc aagaaaactt cggtcgcttg
     1921 gaaggcttga cattggtata ctgtggtgat ggacgtaaca acgttgccaa cagcttgctc
     1981 gtaacaggtg ctatccttgg tgtcaatgtt cacatcttct caccaaaaga actcttccca
     2041 gaaaaagaaa tcgttgaatt agcagaagga tttgctaaag aaagtggcgc acatgttctc
     2101 atcactgaag atgctgatga agcagttaaa gatgcagacg ttctttacac agacgtttgg
     2161 gtatcaatgg gtgaagaaga caaattcgca gaacgtgtag ctcttcttaa accttaccaa
     2221 gtcaatatgg acttagttaa aaaagcaggc aatgaaaact tgatcttcct acactgcttg
     2281 ccagcattcc acgatactca cactgtttat ggtaaagacg ttgctgaaaa atttggtgta
     2341 gaagaaatgg aagtaacaga cgaagtcttc cgcagcaagt acgctcgcca cttcgatcaa
     2401 gcagaaaacc gtatgcacac tatcaaagct gttatggctg ctacacttgg taacctttat
     2461 attcctaaag tataatttta gataataaac cgtctaccaa cagctatgag ggctgcgact
     2521 aatagcttta gtccggtcct cttttatata atggtaatct attatttctt ataaaatatg
     2581 tgaaaaatca ttaaattgaa atctaaacgc attctattga gtgtgataaa ggagaattta
     2641 tggcaaatcg taaaattgta gtagctttgg gaggaaatgc gattctttct tctgacccat
     2701 cagcaaaggc tcaacaagaa gctttagttg aaacagctaa gcatcttgta aaattgatta
     2761 aaaatggaga tgatctgatt atcactcacg gtaatggacc tcaagttggg aatctcttgc
     2821 tccaacattt ggcatcagac tctgaaaaga accctgcctt cccactcgac tcacttgtcg
     2881 ctatgacaga aggtagcatc ggtttctggt tgaaaaatgc tttgcaaaat gctctcttgg
     2941 atgaaggcat cgaaaaaaat gttgcctctg ttgtaacgca agttgtcgta gataaaaatg
     3001 atccagcttt tgttaacttg agtaaaccaa tcggtccttt ctattcagaa gaagaagcaa
     3061 aagcagaagc cgaaaagagc ggagcgactt tcaaggaaga tgctggccgt ggctggcgta
     3121 aggtcgttgc ctcaccaaaa cctgttgaca tcaaagaaat tgaaaccatc cgtactcttt
     3181 taaataatgg tcaagtcgtc gtagctgcag gtggtggcgg tattcccgtc gtcaaagaaa
     3241 acaatggaca tttgactggt gtcgaagcgg ttattgataa agacttcgct tcccaacgtt
     3301 tggcagaatt ggttgatgca gacctcttca tcgttttgac aggtgtagat tatgtatttg
     3361 ttaactacaa caagccaaac caggaaaaat tggaacatgt gaatgttgcc cagctggaag
     3421 aatatatcaa acaagatcag tttgcaccag gtagcatgct tccaaaagta gaagcagcta
     3481 tcgcttttgt caatggtcgt ccagaaggaa aagcagttat tacttccctt gaaaatctag
     3541 gcgccttgat tgaatctgaa agcggaacaa ttattgaaaa aggataagtt gttttactaa
     3601 taagatgtat tctatttcta gtatctttat atcaaattag aaattattct tgaaaacatg
     3661 tacaatattt caaaagatac tagttttaga ctttaatatg gtaaaacaaa tataaataga
     3721 aagcgttttc ttgaatgttt atttaagaaa gtagttggtt ttttacactt tgttagacat
     3781 caggaggaaa aacaaatgag tgaaaaagct aaaaaagggt ttaagatgcc ttcatcttac
     3841 accgtattat tgataatcat tgctattatg gcagtgctaa cttggtttat ccctgcgggg
     3901 gcctttatag aaggtattta cgagactcag cctcaaaatc cacaagggat ttgggatgtc
     3961 ctcatggcac cgattcgggc tatgctaggt actcatccag aggaaggttc gctcattaaa
     4021 gaaacgagcg cagcgattga tgtagccttc ttcatcctta tggttggtgg tttccttggc
     4081 attgtcaaca aaactggtgc tcttgacgta gggattgcct ctatcgtgaa gaagtataag
     4141 ggccgcgaaa aaatgttaat tttggtactg atgcctttgt ttgccctcgg tggtacaact
     4201 tatggtatgg gtgaagaaac aatggccttc tatccactcc ttgtgccagt tatgatggcc
     4261 gttggttttg atagcctgac tggtgttgca attattttgc tcggttctca aatcggctgt
     4321 ttagcatcta ctctgaatcc atttgcgaca ggtattgctt cagcgactgc gggagttggt
     4381 acaggggacg gtatcgtact tcgtctgatc ttctgggtta ccttgactgc tcttagtact
     4441 tggtttgttt accgttatgc ggataagatt caaaaagatc cgactaagtc actggtttat
     4501 agtactcgca aagaagattt gaaacacttt aacgtagaag aatcttcatc tgtagaatct
     4561 acacttagca gcaaacaaaa atcagttctc ttcttatttg tgttgacatt catcttgatg
     4621 gtattgagct tcattccatg gacagacctt ggcgttacca tttttgatga ctttaatgct
     4681 tggttgactg gtcttccagt tattggtaat attgtcggtt catctacttc tgcactaggt
     4741 acttggtact tcccagaagg cgcaatgctc tttgccttta tgggtatcct gattggtgtt
     4801 atttatggtc ttaaagaaga taagattatc tcttccttca tgaatggtgc tgctgacttg
     4861 ctcagtgttg ccttgatcgt agcgattgct cgtggtattc aagttatcat gaacgacggt
     4921 atgattaccg atacaatcct caactggggt aaagaaggct tgagcggtct atcttcacaa
     4981 gtctttatcg ttgtaactta tatcttctat ctacctatgt cattcttgat cccatcttca
     5041 tctggtcttg ccagcgcaac tatgggtatc atggctccac ttggagaatt tgtaaatgtc
     5101 cgtcctagct tgattatcac tgcttaccaa tctgcttcag gtgtcttgaa cttgattgca
     5161 ccaacatctg gtattgtgat gggagctctt gcacttggac gtatcaacat tggtacttgg
     5221 tggaaattca tgggcaaact cgtagtcgct attattgtag tgaccatcgc ccttcttctc
     5281 cttggaacct tccttccatt cctataaaat agtgagtgag gtgattccat gaaaatagat
     5341 ataacaaatc aagttaaaga tgaatttctt atatcattaa aaaccttgat ttcctatcct
     5401 tcagtactca atgaaggaga aaatggaaca ccttttggac aagcaatcca agatgtccta
     5461 gaaaaaactt tagagatttg tcgagacata ggtttcacta cctatcttga ccctaaaggt
     5521 tattacggat atgcagaaat cggtcaggga gcagagcttc tggccattct ctgtcatttg
     5581 gatgttgttc catcaggtga tgaagcagat tggcagacac cgccatttga agcaactatc
     5641 aaagacggct gggtattcgg acgtggtgtc caagatgata aaggcccttc gctcgcagct
     5701 ctctatgcag taaaaagctt gctggaccaa ggtattcagt tcaaaaagcg cgtacgcttt
     5761 atctttggta ccgatgagga aaccctctgg cgctgcatgg cacgctacaa taccatcgaa
     5821 gaacaggcca gcatgggctt tgcacctgac tcatcttttc ctctgaccta tgctgaaaaa
     5881 gggcttctac aggtcaaact tcatggccct ggatcggatc aactagagct tgaagtagga
     5941 ggcgccttta acgttgtacc agacaaggcc aactaccaag gtcccctcta tgaacaggtt
     6001 tgtaacgatc tcaaagaagc tggttatgat taccaatcca ctgaacaaac cgtaacggtt
     6061 ctcggagtgc caaagcatgc taaggatgct agtcaaggta tcaatgctgt catccgacta
     6121 gctaccattc ttgctcctct ccaagaacac cctgctctca gctttcttgc aacacaagca
     6181 ggtcaagacg gcacaggaag acaaatcttt ggtgatatag cagatgaacc ttctggtcac
     6241 ctatccttta atgtcgcagg tctcatgatc aatcatgaac gttctgaaat ccgtattgac
     6301 attcggactc ctgtcttaac tgataaggaa gaactagtag agttgcttac aagatgtgca
     6361 caaaactacc aactccgcta cgaagagttt gactatctag cgtctctata cgtcgcaaaa
     6421 gacagtaaac tcgttagcac actgatgcaa atctaccaag aaaagactgg cgataacagt
     6481 cctgctattt catccggtgg tgccactttt gctcgcacca tgccaaattg tgtagccttc
     6541 ggcgccttat tcccaggagc gaagcagaca gaacatcagg caaatgaatg tgccgttcta
     6601 gaagatttgt accgtgctat ggatatttat gccgaagccg tctatcgact tgcaacttaa
     6661 tcaggcaact gtttctacca aaaaaaatcc cgcaaaatga actgcacccc aaaagttaga
     6721 cagaataaat ctaacttttg gggtgtttta ttatgaaatt gagttatgaa gataaagttc
     6781 agatctatga actaagaaag caaggacaaa gcttcaaaca gctttcaaaa agatttggtg
     6841 tggatgtttc tggtctaaag tcatctgaat ctttgagatg agctttataa atcgcttttt
     6901 tcagtttttg cactggtgtt tcgataaact caaacttttt agccgtggta ttgcctgatt
     6961 ttatagtata ttgaaactag aatagtacac ctctacttct aaaacatttt tagaaatcga
     7021 tttgactgtc ctgatcgatt tatcctgttc ttatttcatt ttactatatt tgagccactt
     7081 cgtctttaac ggctttattc ataagctctt gtaatttttc tttactatca attacttctg
     7141 attttccgtt gtaatttatt gtaataggtt ttaacttacc taatttctcg acacgctcat
     7201 taatttgatc ttttttgaag gctgcatatg tttttcctaa gattttttcg aaaatatatt
     7261 tatcagatag cggtttgcct tcttcttcag cttggttttt gtattgattt gaaacataag
     7321 gaacaaatcc ttcatagtaa cctaatgctc ccataagttc aaaagcttgt tttctaattc
     7381 aaaccattgc aactcagatt tcagcttttc agataaatcc tgctcatcca aataatgact
     7441 tgaaattagt gctgaactcg tttctgtatc ctgtacaggc tgagcaccca taccagcaaa
     7501 aaataaactc gttcctagca agaccgaaca agctcctatt gcatatggcc tcaaagaaaa
     7561 acgctgcttt ctctcaaatt gaaattcttt catcccatct cccatcattc attattactg
     7621 tatattttgt atatcagaaa tagtttgtat tcacaaatct ttctagttat tcccttatca
     7681 ttcctaatta agggagataa catacaataa tttttagtta aatgtatatc gatgtttttt
     7741 gtttttctta ataaacgcaa tacaaaaaga gcctgttgcc aagctctttg tactcaatga
     7801 aaatcaaaga gcaaactagg aaactagcca caggttgctc aaaacactgt tttgaggtag
     7861 tagataagac tgacgaagtc agctcaaaac actgttttga ggttgtggat agaactgacg
     7921 aagtcagctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc agctcaaaac
     7981 actgttttga ggttgcagat agaactgacg aagtcagtaa catctatacg gcaaggcgac
     8041 gctgacgtgg tttgaagaga ttttcgaaga gtattagtct attatttctt ctcagcgcga
     8101 agggctgaca agatttgtgt tcggatatca tccacaccat ttggagtatt tggtaaaaag
     8161 atggtttgat ttcctttaga ggcaaaggta ttcaaggtat ccaaatactg gttggtcaag
     8221 aggatagaca tgatttgttc ttctgtcatg ccaacattgg cttccttgag ttcggtgata
     8281 gactctgcca atccatccac aatcgcctta cgttgttggg caatccccac accatgaagg
     8341 cggtcttttt ctgcttctgc ttcagctgca gtgacaattt taatcttgtc agcttccgcc
     8401 aattcttgtg ctgcgacccg cttacgttgc gccgcattga tttcattcat ggattgctta
     8461 acttctgcat ctggttcgac cttggtaatc aaggttttca cgataatgta gccgtaagtg
     8521 gtcatttctt ctgctacttg gtgttgaacc tcaagggcaa tctcatcttt tttctcaaac
     8581 aattcatcca aggttaattt tggaacagaa gagcgaagag catcttcgat ataagattta
     8641 atctgagatt ctggacgtat gagtttatag taagcatctg tcacgctctg ctcgttgaca
     8701 cggtactgag tcgctacatt catcataacg aacacattgt ccttggtctt agtctcaacc
     8761 acaatatcac tttgcaacaa gcgcaactga atccgtgctg caatcgagtc aatcccaaaa
     8821 ggcaagcgaa tatgaatacc gctattagca accttttggt atttcccaaa gcgttcaata
     8881 atcgccaccg actgctgacg aaccacataa actgtactca gtgtgactat caccaatagg
     8941 agcacacaaa caatcagaaa aatcatgaaa aatattgcca taatggaacc tccacaagta
     9001 tttttctagt attatagcac atttaaagaa ggctgtgccg tttttactgc gatttttcct
     9061 gaaatgtcaa taattagagg tgaattgtcc tattgtcgtc caatctcttg ctaaaataac
     9121 tctttataaa aggcaatcgt ttcttctaag gttggcataa atggatttcc tggtgcgcag
     9181 gcatcaatca aggcattctt agaaaggtat tcaaagtcga aatctttttc ttcaatacca
     9241 agttcagtca gtttcttagg aatacctact gtctcagaaa gcttctcaat ctcagcaatc
     9301 gcataatcgg cacattcttg atctgattta ccttctacat gaagtcccaa ggctttggca
     9361 acattgcgga aagcttctgg tacacgttta gcattttcac gttctataac tggtagcaac
     9421 atggcacagc acacgccatg tggcaaatta tatactgcac cgagttggtg agccattgag
     9481 tgaacataac caagaccagc attattaaag ctcataccac caaggaagat ggcattgacc
     9541 ataccttcac gcgcttcaat atcatgtcca ttttctacag cacgaggaag atattccttg
     9601 ataagctcaa tagcaccaat agacagtttc ttggttacat cataagcacc aggtgttacc
     9661 aaagcttcaa cagcgtgagt cagagcatcc ataccagtag cagcagtcag gcctttaggt
     9721 ttggaaagca tgagttccgg gtcattaaca gagataagag caaggctatt cttgtcaacc
     9781 attaccatct taaccttgcg ttcttcatca gtaatcacat agttaatagt gatttctgca
     9841 gaagttcctg ccgttgtatt gatagccaca actggcaagc cttttttagc agacttatga
     9901 agcccttcat aatcctgtgg ttttccacca tttgtagcca tgatagaaat acaactagct
     9961 gcatcctgtg gagaaccccc gccaagactg atgataaagt cacagccatg ttcttgcaaa
    10021 gcagctaccc catctgtgac atttttacaa gtagggtttg gctctacatc gctaaagatg
    10081 acatattcga ttccttctgt atccagtggt tttaaagtct taggtaaaat atcactgcct
    10141 tcgatgtact tatctgtaac caaaagtgcc tttttatagc caagttcctt gatataagga
    10201 cccacttcat ttacaacacc ttttccaata aggttgactg ccggaacata aaatgtagcc
    10261 atataatttt cctcctgcgc ctccgcgcta tctatttaac atcattatat tactttttgt
    10321 attcgttttt aattatttgt gaattgttta acaaatcttt ttaaaaggat aggagggacg
    10381 taagttcacc tcctatataa acaagtttta tttatgtagt ggtcccaaca actgacatgc
    10441 acaatagtct gctgattcta gatcttctgt tccaaataag gaccaatttt taggtctaaa
    10501 gatatcttcc tcaggtacat tatgcatatt gacaggaatt ctcaacatag aagccaaggt
    10561 aatcaagtct gctccaatgt gtccatatgt tatggctccg tgattagctc cccaattatt
    10621 catgacgtca tagacagact tgaaagcacc ttttcctgtc aaacgtggag caaaccaagt
    10681 agttggccat cctggatctg tacgattatc taaagtatgg tgaacatctt caggaagttc
    10741 aagtgtataa ccttctgcaa tttgtagcac tggaccaacc ccttttagaa gattgagacg
    10801 taccattgtt actggcatat cccccttcgt caagaaacga gttgagaatc ctcctccacg
    10861 gaagtattcg cggtttgctg gtgggaagtc tgtattttca agcatagcct gcacttcact
    10921 ttcttccaac tcccagaatg gtttcataac aggtttgcca tctcgagtag cttgacctgt
    10981 accatccaat gtacaagaac cagagttgat tagatgtaag aagccagctg cagcacgacc
    11041 ctctaaagta tgtcccgtta cacgtttaac agcctctggg ctccaataag tacgcacatc
    11101 agcaaagatt tgtggagtat ttgttaatag ataattaaag agcatagaca caccatttag
    11161 tgaatcattc tctgtcgcaa atacaaatgg ttttcgaata ccattccagt caaactgagt
    11221 attgaggaaa gtttccataa aatctccatt tggaaaatgg tctgtccact gacgttgacc
    11281 ttggaaacca gctactaaag catggtgacc aactgcttct tcctcaaaac caagttcagc
    11341 aagtcttggg ttaccaacca ttaagtcacg tccaatcatg aacatcttaa taacaaattc
    11401 ccattgtcta tctttttctt cacggcttaa aacaaggtct tcacggttat ggtcgaatcc
    11461 ttcttttacg ttttctttca cccatttgag cgcacgttcg aactcttcag ggtcgtaaat
    11521 actacggtcc atacggcgcg tgaactccgt catatctacc gattcatttc gcattcctag
    11581 gtattcttgg aagaaatccg gatttacaat agaaccacca atccccatcg aaacactacc
    11641 cattgatagg taagcagtgt ctctcatcaa gccagttgca agagctgcac gcgcatagcg
    11701 taaaagtttt tctttgacat cttctggaat atctgtgtca ctagcttcct gaacatctct
    11761 tccataaatc ccaaaggctg gaatcccttt ttgagcatgt gaagctagta cagctgcaag
    11821 atagacagct cctgggtgtt ctgtcccatt aaatccccaa atagcatgag gaatatctgg
    11881 agacatatcc atagtttcac taccataaca ccagcatggt gtaactgtaa ttgttgcgca
    11941 aacatttgat tttttaaaca actcatggga agctgcagcc tctggtacac ggccaatagt
    12001 agatggagaa atcacgcatt ccacaggttc cccatctgga tatttcaatg tgcttgaaat
    12061 caaatctgcc acacttttag ccatgttcat tgtttgcact tcaagtgatt cgcgtacacc
    12121 ttgacgacga ccatcaatag tcggacgaat cccaatacgt ggatgttgaa tcatactatt
    12181 ttcctcttat ttttctgttt ctttcatact accatctcca ttatatttac ggtagcctgg
    12241 gtgataattt ttaaaattac ttataagtat aaacctctac ctctgctaaa cttaaaggaa
    12301 ttcctgcgct tctcaaagaa attctaacct tttttccctt cagtcctttt aagtctatgg
    12361 atagattatt gctttctaaa ctgtcaatat gttgtgtaaa aacttcataa tcatttgaat
    12421 catatataat aatatcaaaa tttgataatc tctgaatttc agcatctgtt ctattataaa
    12481 taattaactt ttctaattct tcggtttgtg ctaaatctat ctcccaccat gaaggagaat
    12541 cttctttggt atgagtcact gaatgatgac cataatctcc gtttttattt ccatcaactg
    12601 ctagacgaga aaaaccattg taatccgttg aactctgtcg agtttctttt gttaatgcaa
    12661 tattagaaag tttcttcgga gttctctttg aaccatagac ttcaacttcc gctaaactta
    12721 acggaatagc tgctgatttt aattctattt tgactagctt agcccctaca gaaggtactt
    12781 ttaaagataa caaaccatca actactttat caaaatgtct tctaaaaact tcttctcctg
    12841 atgaagatag aaaaataaca tcaaaattag ataaacgctg aggttcggca tctgttctat
    12901 tataaatatc aactttagaa accgtaaact cttctcccag atcgacctgc caccaagcgt
    12961 tagaatctgc cttagtatga gtcaccgaat gatgtccgta atctccatct ttattaccat
    13021 caactgctaa agcagctaca ccctcataat tggttgaact ttgagtagct accttatttg
    13081 tagagactac tttatcttct gttattttat ctatatcctc ttcagattgc ttaccatctg
    13141 attctctaaa aacctctact tctgctaaac tcaaaggcac tccactcgtt agtaatttaa
    13201 ctttaatata ccttgctcct ttttctttga aatctagact aacagattca cccgacaaat
    13261 tattaacatg tttcttagca acttcgtttc tattattgtc atatagaatc acatcaaaat
    13321 tagatagacg ttgagtctca gcatctgtgc gattataaat tttaacaagc ccaactttat
    13381 ccattttttt caaatcgact tcccaccaag agggattatc tgccctagtg tgtgttaccg
    13441 aaccagagtt aaaattacca tttctgttac catccacagc tctattaggg tctccattgt
    13501 aatctacaga actttgtgtt gttggttttg catatgctat attcaaatta ccatcattaa
    13561 atttatctgg tgttgggaaa gagactggac ctgtcccctc agtatttata gacatctcta
    13621 ccattccatt atgattaacc gtaatggtat aaacatgggt attctcatcc caattttctg
    13681 tataagtata atgattttta tcgccactta tatttatctg aggtttatga ccagtatgcc
    13741 cttttaatgt aagtgttgtt gtcctaaagt cattatctac aggattgatg gaataatttt
    13801 tgcttatcca gttcgctaac tctaattctt ctttcttcca actttttgat gcatcaaaat
    13861 ttcctgataa tgcccacata gctgtcttat ctgtcctgta attattcaat aaaatatgta
    13921 aattatttgg attttcttta acaacagcgt aagtatgtgg cgtcaaatct aacgataacg
    13981 actttgtatt attagtatac ataggcaaca ttacttgctg atttttattg atattagcat
    14041 tactattata aatataccag gaattaccta cacgctgagc atacccatct ccttcataaa
    14101 gttttggata aagcgagttt aaatagttga ctttactaga caattcctca ctatttttag
    14161 tcaaaatttt tgcattaggg aatatagatg aaatcttttc cttatcaatt ttctcatgta
    14221 ttacaggaag aatatgatat ctcccattat tatataaagg cattgtttca tcattcgaat
    14281 aaagtccttg ataaaatccg tttaatgaac taatcctacc ttctccattc caaaatacag
    14341 cttttgttct atttacaact tcttccttac ttggagctgg attttgtata gcatgtctaa
    14401 agaaaggaat aataccttta gtaaatgctg gagttggtac atcatttgtc ataaatgtat
    14461 acgcggcaca ttcgaaatta taaactgtgc ctcccccagt atatacattc atcatttcca
    14521 tagcaatcat tgattctggt tccgatgcat aggatctcat atccctagct cttccagttt
    14581 caaatgtgtt tgtataatgt ttttcccacc atttccatgt atctgttgat gagccccagt
    14641 tatcacataa gccactcaac caaaatccac taacgataga atctgtaccc gcatcatctc
    14701 ttattggcgt atttttagtt gccaacacca aatttttatg atatttttga ctcgcttcaa
    14761 agaatgtcgg atcattcata atagtttccc agaaccattt ttcatgatca tgccagataa
    14821 aatgcgctcc atatttggca caaacttcca aatatttagc actatgcgga gctaactggt
    14881 tattgtaaat ccaataattc tcaatattta aaacaccttt taacacacta tacttttgga
    14941 attgttcatc taaccactct ggaggaactg tattacgctc tccagccgac ataataacca
    15001 agaaaactgg aatgtttaga ctttgagctt cctccaacat cttgacatac cattctctca
    15061 attctttcgt atctcgtgga atacaacttg ttggtttaca gacttttgca ggatgaagtt
    15121 caatcgctgc atatggcttt acatcttcag gaatagcttc ccatgctccc ttcaatgtgt
    15181 tcccccacca taatccgtta ccattatcat tgccatacaa cggagaaagc aacaatggcg
    15241 attcattatt aatcgtcgtt ctcatttgaa cacgattatc agccttaact cctgctaaca
    15301 atccaaaaca taacatccca atacatacaa acaatattgt gattaatttt ctttttattt
    15361 tttctttatt catgacttat acctaaaaat ttaactcaat aattttttct gaattaaata
    15421 ccaattcaat attttgtaca tcagtatttt tgccatatat tcttactctt actttttgat
    15481 ctttgtttcc tccttccaat tttaggaatg ttatatcccc atttttccaa gcaaacgata
    15541 ccttatatcc tcctcttact ctgaaacctt tcacttctcc ttctgaccaa gcagaaggta
    15601 aagctggaat tagtgataac caattatgat ggctctgtac taataattca caaattccac
    15661 tcaccaaacc taaattacca tcaatttgaa atggtggatg gtcaagaaat aaattgccaa
    15721 gagtcgcatt atttaacaaa ccattaatct ggttataagc aggttcacct tgatatagtc
    15781 tcgcaaaaaa atgaatcagc catgcagcac tccaacctgt ttgtgtacta gcatgcaaac
    15841 cacttactaa ccaattatta atcgcttgct ccctctcctg tgaagataaa aaattagcgt
    15901 ttgataatct cctattgata gtgattttag ctgcttctgc taattccgga gttttatgaa
    15961 tatcaatctc attataagga taaagcccaa atagaggtga aatgtgtcta tgcccaggct
    16021 ctacttcttc ataatcttct aaccattctt ggatttgccc attactacct atttttgttt
    16081 taggtagttt cttttttaac tccttcacac gactaataaa atccgaattg tctcctaatt
    16141 gttttgcaat gccaatgcat gaatcacaaa aatatcttag aatttgatta tcaattgtag
    16201 atgatagaca agcatttcct tcaataccat tttttaagcg atatttattt tccggtgaga
    16261 cacttggacc tgtcatcaag tagccatcca cctcaaataa ataatcttca aagaaaagaa
    16321 atgcttcttt tatcatttca aaatgttccg taagaatacg ctcatcttgg aaatataaat
    16381 agtgttccca aatatgagta cataaccatg gaatagttaa tacccaaatt gcagccccca
    16441 tggcatgaga ttggggagcc gtatcgccaa aaccatccgt attatgatgt gctgtaaaac
    16501 ctctagctcc atacattttc ttagcggtta gtcttcccgg ttctctcatt ctttcgagca
    16561 tatcaaataa tggatattct acttctggta aatcacatgg acctaccatc caataattca
    16621 tttgagtatt aatattaatc gtatatttag aaccccaaat tggatttaat tcatcacacc
    16681 atattccttg aagattggca ggtaaaccat tcggttgact agacgatatt aacagatatc
    16741 ttccataatg aaataacaag ttagtcaagt agttactata ctttttagtg ttttcaagaa
    16801 gtagattcgt tggaatgcta agacaatctt tactatagtc tagtttaaaa tcaactctat
    16861 taaattgctc ctgatatttt tttacatgtt catctttttc tgtaaagtaa tcaatactac
    16921 taaattctcc ctgaagagaa gaaatatcta tatttcccca ataatccgtc attgatttga
    16981 gataaagaaa tacctctgta gcattccgaa taactattgt ctctcccaat acacttactt
    17041 caccatccgt aaccttagaa tgacatacta ctttaaactg aacacctttt ctacctccag
    17101 cagaggccga cattaaaatt gtacttgaat ccagtttaga tacttcgtca ttaaaccgtt
    17161 tatttctacc caaattaatg tttaaattta atgtgttttg aactgatgac actatacggc
    17221 aacataaaat attcttatta aaactcgtaa aatattctct ttttatttgt aaattacaac
    17281 tattaggctc aaacacaaca ttagaaatag ctgtatctaa atctagctct ctttcataca
    17341 atgaaagagc acaagactga atatctatat gctcaatgta aagttcccca agtaattcat
    17401 agtggctttg atctcttggg gtagcaaaca ttgttaactt tatcaattct tcggctttct
    17461 gaatttctcc atctaaaaga tattcccgaa tttttttaag atgcaatagt gagtctggat
    17521 tatttctatc tgactttcct ctataccaaa tagtctcatc gtttagttga atacattctt
    17581 ttgtagctga accataaatc ataccaccta aatgaccgtt cccaatcggc aaggcttcat
    17641 tccaatttga ggcagctttc ttataccata atttcactct ttacctctct tccaaaatgg
    17701 aggtgatatc ccaaatacca cctccacact ttctactact tcaaaattcc taaaacaact
    17761 aggataactc caatagccat aacactaaag attagtacaa ccggattctt tccttttcta
    17821 attaatttac acattaataa agtcaaagcc atagggataa atcctggaat taattttgta
    17881 atcatttctt gaacagaaat aacaagttcc ccctgcttaa attctaatcc aaaattaata
    17941 ccaaccattg atggaatcaa accacccact agtactagcc ctaatgctgt cgccatactc
    18001 gtaacgcgat tcaatgttcc ttttgtatta ttttcttgga taagactaga acccttagta
    18061 tacccatatt tcaaaccgaa atatttaaca ggaatattaa taatattaaa caatattaag
    18121 gcgataaaga tacctaaagc accgccgtct ttagaataag acgcaccaat actaaaacag
    18181 ataggaacta gtgtcagcca gaacaaacta tctcctagac cagccagtgg ccccatcaag
    18241 ccaactttaa taccagtaat tgaagctgca ccttcatttc cttcttgttc ttccatagcg
    18301 gaagtaactc caagaataaa tggtgccgct gtttgatgag tattgaaaaa ctcaaggtga
    18361 cgtttcattg caggagaagc tgaatctttg tctgggtata gttttttcaa tactggaaga
    18421 attgtatata gaaaacctag gttctgcatt ctctcatagt tccatgaaga accatacata
    18481 aaacttcgtt tgaaaacttt tttaagttca actttagtta ctttattatt catcatagat
    18541 caagatcctc ctcaacatca ctacttgaag ttgtttcttg tggtttactt ccaatcatat
    18601 cgtagaagta tgctaccgca ataccaacta gtgagatccc aatggttgac attcctccat
    18661 acacagaaca aatgaatcca atcaacaaga atacccacat atttttcttg agcatcatat
    18721 ttaaaagcat accaaaacca attgaaggta gtaaagcacc tgcagagtta agtccctgca
    18781 taacgattgg tggaacctta gcaaacaagt ctgccacata ttcccctcca agcataatag
    18841 ctaggaaaat tggtacaatt ttaaataacg tgattaaaac caaacttgaa taatgataaa
    18901 aaccgatctt tgaataatct ccgtttttag catcattatc agcttttttc acaaaataaa
    18961 catctaaagt ttttgcaaga acatccaact gttgggtagc aactgcaatt ggaactgcta
    19021 tagcgatacc agcagtttct ccttgaccag ataaaatacc aaatgcagta ccaataatcg
    19081 cacctgaaat agtatctggt ggagtgtaac ctccaatacc tgttacaccg agccaagtta
    19141 attcaagagc tgaaccaata agaacacctt gggtgaaatc tcctaagact aagccaacca
    19201 ttgcacttgt aactaacgga cggtgaatca taaataacgg tccattatag tcaatagtgg
    19261 caataatccc aactaatgtc accagtaatg cttgtgtgaa tagcatatac tgtacctcct
    19321 taaattattt ttataaaaat ttttccaaca ttgtaacttt atcattagga accatttgaa
    19381 tttcaactct cgttccctta tcaactattt tcttaaaata atatttgtct tcttcaccta
    19441 gagctacacc tcccgttacc ttttcatttt cctccgtttt actcatctgt cctacattga
    19501 ggtactccaa ttttaaattt ccttcaatag aatcatacac atcttttgga tttgtatata
    19561 tcagcattgt tctctttttt attggcacag agtttaaaac ttccacaaac cgttttacac
    19621 taaagaaaac aatttttaaa cctaccggtg cagaaatctt taaaatagat tgtcgtgttt
    19681 tatcttctga gatgcgatca ttaacaatga taacttgctc aatatcatac ttttttagcc
    19741 acgtagtgac aacttgacca tgtaccagac ggtcatcaat acgaacaaac tctatagaca
    19801 taaatcctct tcctcctcta cgttaagttg ttgtttaaca ttaaacaaac tattttgagc
    19861 attttgaaca atttcttcta aattgatttt tgaatcataa cttgatatta attccactag
    19921 gagagggata ttaaaccctg ttacaatatc aactgaatcc aaatttaaaa accgtgacaa
    19981 agccacatta ttaggacttc ctccaatcaa gtcagtcaaa acgataacct cttgatttga
    20041 gtctaacagt tcatccactt gatttttaaa ataatgttca aactctacaa tattctctcc
    20101 aggcatcaaa cctaatgtcc taactctatc atttgttgtc tcaccaacaa acatttctgc
    20161 ggtcatgaga gctccgctag caaaattacc atgactggca actaaaattc ctctattaaa
    20221 cattttctct ttcaacaact actcctttct taaggataat attagcataa agtgaagttt
    20281 ctcctgtagc aacaatagca taagctttct tactacgttc ataaaagtct tctcttctaa
    20341 gataagtaat cgttttaaga tctgtaccat gaccttcaat catctgtcta taggtacccc
    20401 atatcttagg aatatcatca cccgaaacaa cgttcataaa ctgaattgaa ctatcgacgt
    20461 aactatctaa tggcattaaa tacagaatgg aatctaataa ttctggaata tttacgccat
    20521 cacaacgaat tagcttattt gcacatgagg cagaaggata attcgcgtca gctaatacta
    20581 tttcatctcc atgtcccatt tccattaaag tcttcaataa atctggagaa atatttttcg
    20641 gtatatgttt taacattaat atcctcccta ttttctctga ccgtatgttt taaatttttc
    20701 tgccattaaa tccatctcct catcaggaag aactactggc tctccaaaat tcttagctaa
    20761 acaataaatt tttgcacaat attcaacttc ttcaacaata ttaaatgcat tcaataaatt
    20821 ttgtgcacct gctaaaattc catgattcgc tagtaaaact gctctacgac cttccattgc
    20881 tttagctgca ttcacagcca attctttcgt accatatgtt gcatactcag ctacccgaac
    20941 atctttccct gccactgcaa tcatataatg actagccgga agtggttctc tgagacaagc
    21001 taatactgtt gcataagttg tatgagcatg gataattgca tcgatatcat cacgagtttg
    21061 atattgaatc aaatgcatat accattcgct agatggcaag cgttctccct ctacaacatt
    21121 tccattaata tccatcacta caatatcgga ttctttgatt tcaaagaaat caatacccga
    21181 cggggtaatt gccatcaatt gtttttcacg atcgaaaaca ctgagattcc cacctgttcc
    21241 tttcgtcaaa tctgtttcta ctagcttctt gccatactta attaattctt gttttacatc
    21301 tgacattctt gtcctcctca tatcaaaaca ctaaatattt ttcaattcac caattatccc
    21361 cccttcttgt acaaaactta agaaggggga gggaggcaaa tttacaaact ctcaatcttt
    21421 ttaattcttt tgggagtaat aactctctaa ttgtagaaac tcctcaataa catggtgagc
    21481 ttcttccatc ccttttaatt gtcccatagc tatgagctgc acaacaatat tccccacagc
    21541 tgtaccctca gtcgaacctg taagaacctc tttaccagtt ctatcagcaa tcatttggtt
    21601 aaagtaactg gctctagcac ctcctccaat cacatatatc ctcttataaa ccttatgagt
    21661 tagttcttct agtaactcta tcgctttcct atacgtttca gctaggcttt cataaacaat
    21721 cttaaatagt tgtccatctg tccactctct agtttcatga tgataagcta gatattctgt
    21781 caaagtcttg tgcatatcag attctgttgc aaattcagtt gattcagtat caatcagagg
    21841 aagattttct ttttctttct ccaccattgt cctaatatca tcaaaagaat aggctttccc
    21901 tcttcgttca aatgaacgtc ttagttcctc tatgatccac aaccctgtac aattcttcag
    21961 aaatgtaatc actccatctt taccgacttc atttgtaaat ccataactga aggattcggt
    22021 agtaagaatc ggtgaagtaa gttccactcc aaccaaagac caagtacctg atgaaataaa
    22081 taaactacct tctgtcttag gtactgagac aatcgcactt gctgtatcat gactacaaac
    22141 attcacaaca ggaatatcgc ctaaaccata ctcctttttt atccttccaa gaacatttcc
    22201 ctctgaaaca atttcaggaa gtaaagatga atccaattca aatagtttta agatattctg
    22261 attccaattt tgactcctag gatcaaataa ttgagttgtt gaagcaatgc ttttttctgt
    22321 agcaaactta cctgtcaaga gataattaaa caaatctggc attaaaagaa tcttattggt
    22381 cttatagaaa gagtcaggag attcttgacg tgccttaaag agttgaaaca aggtatttat
    22441 ctccataatc tgatttcctg tctctgaata cagtttttct aattcagtca tttcagatat
    22501 ttcctttaac actccctttg ttctttcatc acgataatga acaggttgtg ataacagctt
    22561 accttcatta tcaatcagtc caaaatcaac tccccatgtg tcaataccga tagataaaat
    22621 cttgtaacta gtattagcca agcggatact ttcaagaatt ttagctagta gaaagtcaat
    22681 atcccaagat aaatgccctt ttactctaat aggtagatta gaaaagcgat ttatttcttc
    22741 cattactagt ttattttcag aaaggtaacc aacgattgct cttccagaag tcgcacctaa
    22801 atctattgct aaaaccgctt ccatttttct tctctcttcg tccttgatta agtttattat
    22861 aatccccaca ttttattttt tcaatatgtg aacgcttact tggataatgt tcaaatgtta
    22921 acccaatatt cataaaaata attcaaattc ctccaatttg tgctataata tagttgaaat
    22981 gataaattag gaggagctta tgataaaaga tgaacgtgta cttgaattga ttgaaattat
    23041 taaaaagaaa aaaagaattg ccgtaaaaga gctggcagaa atcactttct ccagcacaag
    23101 taccttacgt cgtgatttaa ttttcttaga aaatcaaggt cttatcaaaa gaaagcacgg
    23161 atacgtgacc ctgtcctcta tgaacacaat tgaactttct catcaaatac gggaaggaga
    23221 aagtactagg caaaaaagac taatcgctag tctcgctaaa gactttattc ggtcaggtat
    23281 gtgtgtttat ctggattcca gtacgactgt ctacgaactt tgtccctatc tttctgaact
    23341 tgataatttg attattttta caaatggttt acatactgca caaaccctat ctgaaactgt
    23401 taaagatagc tccaaaatct ttatcacatc tggcgaggtc aaacatcaat cctgttccgt
    23461 ggtcaattac gataaggaaa attctttatt agatcatttt aatatcgatt tagcattttg
    23521 ttcagcaaga ggtattgatg accaatatgt ttatgaagct tctctcagcc aagctatttc
    23581 aaaaaagaat attattgaca aagcccatga aaccatctta ctgattgata gctctaaatt
    23641 ttacaagact ggatttttta aaattaatcc cctatccaaa tacacaacct ttatttctga
    23701 caccgtgcca gatcaaaaat tattagacgc agtcgaatta tttgatggag aatgggtttc
    23761 tgatattcaa tgaaaatcaa ataggaagct agtctcagct tgctcaaagt acagatttga
    23821 aaactgatga agtcaagtcc cccatggaca cggcaagacg aagctgacat gatgtgaaga
    23881 gattttcgaa gaatactcgt tctgtctgct gactgattta caaatattca ccacatgaca
    23941 ttattttctt agcaacccta aaaacagagg ctggacaaaa actcaatttc aagagaaatg
    24001 gggcaaatat tcccacaata aaacgcatag tatcaagttt ttgcacacct gatattatgc
    24061 gtttttctga ttttaaagac tttgtcgcct agaccctata tttcaagaat taagtgacct
    24121 tgatgatcta aatgtattgt ggtatactat ttttgtcatc ggttaccgat tacgttccta
    24181 tgggttcgtg agaggaggtg aaactaatga gccttatcat agagctcgct cttactatta
    24241 tagcggatgt tatagctgga attatcttgt atttcatctg cagatggcta gatagtaagg
    24301 agtagaccga atgactagcc tataaacacc cgttaaatct ctaagatacg tcaaaaaagc
    24361 ccttaactac ggccatagtt aggggctttg gtgttctaat gaaccttatc aattaactac
    24421 attttagcat ataatctaca atagttcaag cattaagtga ggttgatgat ctaaatttag
    24481 tgtggtatac tatttttgtc atcggttacc gattacgttc ctatgggttc gtgagaggag
    24541 gtgaaactaa tgagccttat accagagctt gctcttacta ttatagcaga tgttatagct
    24601 ggaattatct tgtatttcgt ctgcaaatgg ctagatggta agaagtagac cgactgacta
    24661 gcctataaac acccgttaaa tcactaagat acgtcaaaaa agcccttaac tatggcacta
    24721 gttaggggct ttggtgttct aatgaacctt atacactaac tacattctag catataagtc
    24781 ctaatatttc aagagtttta tttatttttt caggttccct tagttctgaa aggtctataa
    24841 tgaagttagc catctagtat caaaaaaccg actagctctt atgaactagt cgatttctca
    24901 tcaatgcgcc aacatttctt gggcgatttc ttggccagat aggttatctg ggtagtaggt
    24961 tggccagttg tccatttctt caaagagggc ttcttggctt gtgcctccaa agaagatatg
    25021 gaaatgttct gccttaactg gggcgatatt gtggtcacta aactgaacat acttgaattg
    25081 tccagcgtca gcatctgtgg cttcaaagag gaaacgcacg ccacgattgc ctttcttgta
    25141 agtcaaaatt ttcttaccga catacttgta agtgtatttc ttgctttgtc caccttgaac
    25201 aaattccata gtattatcag taatgttaat cttagtcaca tctgtctgat agccttttgt
    25261 atagtaagcc ttgtactcag cctgggtcat cttaccagtc aacttagcct tgtagtcaaa
    25321 gacttggtca aacgtgccgt cttcaaggaa aggataaact gattgccagt tacctgcata
    25381 gtcactcaag gtgcggtcct tgacagctgc atcctcgaag taaccatttt ggactgtctt
    25441 ggtatcctct gccttttcag gctcgattgc tgggccttct tggtctgttg tttgtttcaa
    25501 agccttgagg tttttctcca tgatagagat gtaattttct ccagccttgg tgtcctcttc
    25561 tgtcagactt tctaaaggat tgaggacatc agttttgaca cctgcttctt ttgaaagtgt
    25621 gttagcaagg gcttgtgagg cattttcttc aaaatagata taggcgattt tatttttctt
    25681 gacatactct gtcaattctg ccaagcgagc agctgatggc tctgcatctg gagaaagtcc
    25741 tgagattgcg acttgtttga gtccatagtc caaggcaaga tagttaaagg ctgcgtgttg
    25801 agtcacaaag ctcttttgtt ttgcttgaga caagccttct acgtaagcct tatccaaggc
    25861 ttgcaatttt tcgatatagg cagctgcatt cttctcaaag gtctcttttt tatcaggata
    25921 atctgctgac aagctgtcgc ggatgtgctc tactagttta atggcacgaa ctggtgataa
    25981 ccaaacatgg gggtcaaact catggtgatg accttcttct ccatggtcat ggtccccctc
    26041 ttcttcctcg ccacctggca agagcaacat atcgcctgtc gccttgatgg ttttcacttt
    26101 tttcttatcc aaggtatcta gcaatttagg tacccatgtt tccatgtttt cattttcata
    26161 aacgaaggta tctgcatctt ggattttggt aactgccttg gcagatggtt cgtattcatg
    26221 aggttctgtc ccagcaccga ttaggagttc tacattagcc gtatctcctg cgacttgctt
    26281 ggtaaattca tagacagggt aaaaggttgt cacgatattg agtttaccat ctgcctgttt
    26341 ttgattggaa caagccacta aaaacaaggc acatagactg gctagtaata agctaatttt
    26401 tttcacgttc gtctcctatt tgataaaacg tcttactaaa ctgattagta taaagacagt
    26461 tacaaaaata atggtaatac ttgcacttgc aggtgtttct gcatagtagg aaatgtaaag
    26521 tcctgctacc attcccaaaa agccaatcgc actggcaagc agcataaccg atttaaagtt
    26581 tttccccaga cgcagggcaa tactagctgg caagaccata atggtcgata ccagaagagc
    26641 tcctgctgca ggaatcataa gggcaatagc cacccctgtc accatgttaa aaagaatgga
    26701 catggtacga actggcaagc catccacaaa ggccgtatct tcatcaaaag ttaagatata
    26761 cataggacga agaaagagaa aggtcaaaat caaaacaacc gccgcaatga caaagaggga
    26821 aatgacctgt tcttcactga tagtcacgat cgaaccaaag agatattggt ccaaactcat
    26881 tgaactcgag cttttaccct tgctcatgac aatcagagaa acagccagac ctgttgacat
    26941 gaggatagct gtcccgattt ccataaagct cttgtaaacc gtacggagat actccagaaa
    27001 gaccgccgca atcaagacaa tggcaatagt agaaacagtt ggagaaatcc ccaaaaccag
    27061 accaaaggct acacctgaaa gtgagacgtg gctaagggta tcactcatca aactctgacg
    27121 acgcaagatg aggaaggttc ccaataccgg tgagaaaaga ctcatagcaa taaccgccaa
    27181 aaaggcgcgt tgtataaagt cgtaagataa taaactaagc atggctcacc tcctggccat
    27241 tctcatgaac gttgaaacaa cgccatggcg agtcttggtt acggactaga tgaatattgc
    27301 gatccgcata atccttaact tcttcagggt catgggtaat catcaaaaca gccttgccat
    27361 gatgatgggc gctgtggtgc atgagttcgt aaaattcatt tttacttcct gcatccatcc
    27421 ccgttgtcgg ctcgtctagg ataaacacat cagggtcaga agcaaacata cgcgcaatta
    27481 ccgctcgctg cttttgtccc ccagatagag accccaagcg tttgtctcga tgttcccaca
    27541 tgccaactga gtccagactg gccttgatat gctcctcatc atgagcattc aaacgacgga
    27601 accagccttt tctcggatag cgacccgact tgacaaattc atagaccgta cttggaaaac
    27661 cagcattaaa actggcaatt tgttgaggaa gataggctat tctcaatttc ttaccttgcg
    27721 tatttgtctt tgaaatagcc acctttccaa tgcgtggttg cagaattcca agactggcct
    27781 tgatgagcgt cgtcttagcc gctccatttt ccccagtcaa ggtaacaaat tccccactat
    27841 caacactata attgatatgt tcaagaacag gctccttatc ataatagaag gacaaatcct
    27901 ctaccgtaat atatctcatt atttgatttc tcctactaaa gcagtcaaaa accgctgaat
    27961 cactttttgt tcatttggag taaactgagt cgccacttgt tcataggtta aaagtgtatg
    28021 ctcatggtga tggtggtgct cctcagcgat tggacgagcc aagtcagtca actgataaaa
    28081 aatcacacgc gcatctttag aatctttaga tgtttccaac atcccttcct tgaccaaaga
    28141 cttaatggcc ttggtaactg ccgcctgact gacattgaga cgacgggcca attctgaatt
    28201 tgttaaagat tcctctgaca agagcataag gatatgctcc tgagtattgg tcagggccac
    28261 ctcgctagtg caatgaccta ttaggatttc atgctgattt tccgcctgca aaatcacctc
    28321 attcaaaaaa gcattgatat cctttgctag ctgtctcata tctgactcct ttccttttag
    28381 acttctcttt tttaagagaa aaatactatt ctttgacatt ttgtttacca gttaattata
    28441 tcacaagcaa aaaaagagtc aagaaaaaac gtgaaaacta gtttcattct tgaactcttc
    28501 tatattatat tatctattga aattctttga catctccatc ataagtcgcc caatctttgc
    28561 tgaaaaagcg ctcattcaga tggtaagtcg gagctggtgt gggattggat aggaaaggat
    28621 caactgcctt gtcaaaagcc aaccaaccca accaaccaag gtgaatggtg tccttcataa
    28681 agaaaggctc cccaccgtcc ttagaaaaat ctgctatatt ggtaaaacct tgactttcta
    28741 actggtagcg aatcttctgc accgtttgtt ggtacatatc ctctcgtaga ccagcatagt
    28801 ccatccattt tttattaaca ggtggaatga taaaaatcgg gtttacctta gatttagaaa
    28861 actgtgttaa aaccaactgc aagtcattat actctggcga cttgagatag gtaaagcttt
    28921 tctgagaatc ctttaatttc ttcaaatcct tcttgatctg ctcattatag aaataatttt
    28981 ccattcccat ctcattattg gaagtatttt tttcagcatc tgctttgaca acatcttcta
    29041 ttgcttgata agaaaactgg tctggcaaga tttttaaata cttagctaca tgcttatcgt
    29101 agttaacata gcctctaacc gaaaactgac caaaaaagga ggcttggcgt tcattaaaac
    29161 gagccaataa ttcaatcatt tcattgtctg ctgtcgacaa ttctccttta cttgccaact
    29221 tctgaaccag gtccttcata gctacgtttg ggaactgttg cagtaagcga gtcgctgcat
    29281 attgactagc ctgatcccca gattgatgtt tcagaaaact agtcaactgg tctccattaa
    29341 aatactgctg gaaggctgct ggatcatagc catttttact gaaccactga ggtgagataa
    29401 catacacaac ttgtttattc tccagctgtg gtaacatctg ttgcattcca aaatattggt
    29461 taagcgatgc agctcccccc tgtcctaaaa gataaggacg gtaggaacga ttgtatttct
    29521 cagctaatac cgcaggatga gcaccgtcaa aacgaagcca ttcactagag ccaaagaagg
    29581 gaacaaaacg cacatttgga tcagatagtg ctctgacttt ttgacttcgc tccttaaaac
    29641 tatcgatagt agtagccact gctgaacgct tttcagctcc tagattatga tgcatctcag
    29701 taggataaaa gaaaatgagc agaaaaacca acaaaccagc gatcaagacc ggtccgaaga
    29761 tcatccataa gcgtttaagc attttgtagc tccacaatac cagctatgat tttattagct
    29821 gtattccagt cgtcacgacc aaactctgtt acagggacac gaatgtcaaa acggttctca
    29881 atctccacaa tcaactcaac cgttcccata ctatccaaga cacctgcatc aaaaagatct
    29941 tcatccatca tgtcagaaac atcttccata aacaactcat caataatttc aataacttct
    30001 gatttgatat ccatatttta tttcctttta ttttttaaac catagattat tcaagaatcc
    30061 agaaaagatt aagaatgaca acatgacaac atggaaagtg acaaccatgc caagcaactg
    30121 aatccagcga ttctcaggta gggcagcctt ccctgctttt ttccgttcct tattgagcgt
    30181 ttttttcttg cgaacccagg catcattgat gaccaagcct agtccatgaa agagtccata
    30241 ggcgatatag taccaggtca caccatgcca aaatcccata atcagcatat ttacaatgta
    30301 ggccatgctt gaggttacat tacgattttt aaagactttc tttctggtta acaccatcac
    30361 cattcgcata aagacaaagt cacggaacca gaaggacaga ctcatatgcc agcgattcca
    30421 aaactccttt aaatcccttg ataaaaaggg cttgttaaag ttgatagggc tacggattcc
    30481 catcaagttt gagatggcca aagcaaacat agaataacct gcaaagtcaa agaagagctc
    30541 cagaccaaaa gtatacataa ctgccaaggc atagagatta aagaagccac ctgactgcaa
    30601 ggctaaattc ttcagaggag gtagtaaggt ctctcctaaa acatgagcta ggataaactt
    30661 atacaaaaag ccccacatga tatagcggac agattcatcc agcatatcca tcaactcatc
    30721 tcgctcagga atagcctgat aattttcatt aaatcgctta aagcgatcga ttggaccact
    30781 cgagaaagtt ggcatgaaga gaaggaaacg gaggaattcc cagagggtaa aatccttaat
    30841 cactccatct ctcagctcga tgataatccc aaccgaacga aaggtcaggt aagaaattcc
    30901 caagaaccca agcaaagact gcgttccatt gatagctggt tgcaccttga caaagataat
    30961 cggaagtagg gacagaaaac taactaagta gaagacccac ttgccatcct tgctttttcg
    31021 ataatgcttg tagaaaagca ggagcaatat ttcccagcaa aggtaaatac ccaaggcagc
    31081 tagttgattg gtctttccac ccaccaacat ggtgacaata aagaagagac ttaccaacac
    31141 ttcataccag gcaaagcgtt tcttgaaaaa gaggcctata aagatgggca aggttgcagc
    31201 aatcacataa acaaaatact gaggattgcc atatggctct aaatgaggaa gctgttgaaa
    31261 aaactccatc atctcttatt cacctcgtta atcaatcctt tgatgtcaat ctttccattt
    31321 ggagtcagtg gcaaactgtc tcggtaaagg aatttagatg gcatcatgta ggacatcatg
    31381 atgtctgtca ggtcttcctt gatggccttg gtaatatcga tatctcgctc aaactgctca
    31441 cgaacactgt cttttaagat gacataagcc aatagatttt gtaccttgtg gtccttgtta
    31501 tagcgcggta ctgcgacagc agattcgata aagcgagact tgttgaggtt ttgagagaca
    31561 tcttctaact caatgcggta accgttgaac ttaatctgga agtccatgcg tccgccgtag
    31621 agaagcaagc cctcatctgt tatggttccc acatcgcctg tgtgataggc tggcagacct
    31681 tcaaactcaa agaaggcttc tgctgttttt tcaggattgt tcatataacc ttttgaaaca
    31741 gctggcccag aaacaatgat ttctccctgt tcgccatttg gcagtttatt tccttcctcg
    31801 tcaatgataa aggttggaga atcagccttg gtatagccga ttggtaggcg tttgagagtc
    31861 gctaacatct cgtctgtcac ggcaactgct gacagagcta ctgtcgcttc tgttgggccg
    31921 taagcattga tgatacgggc atttgggaaa cgctcgcgca gtttttgagc tgttttgacc
    31981 gtcaattctt caccatcaaa gtagaaatgc gtgattccag gcattttttc actgttgaag
    32041 tattcagaca acatggccat atctgcaaag gatggtgttg atgtccagat agcgattggc
    32101 aatgaaaaga tagccgcaaa gagttgctta aagtcctgag tgatgactga aggaagagtg
    32161 aaaagcgtac caccaagtgc caaggtcggt gcccaataca tgacagacaa gtcaaaagaa
    32221 taaggtggct gtgccagcat ttgcggacga ctcggtgtcg caaattcctt atccgtaatc
    32281 atccagtttg taaagctgag gagattatca tgtgaaatct gcactccctt aggcttacca
    32341 gtcgtaccag aagtaaagat aatgtagtaa ttatcatctc ccttgactgg atgcgtgatt
    32401 tcatagttgt tcccttgggc aaaggcttct tgaacctgag ctagatttat cattggtgta
    32461 gaaacctgct ccaagggaaa ggctgaaatg gcaataatca agcttggctc tgctacttct
    32521 aaaatagctg aaactcgctc caaggccgaa tggctatcaa ttggaatgta ggcatgacct
    32581 gacttagtca gcgctacaaa ggttgccaac atttcatatt cttggccacc aaaaacaacc
    32641 acaggagact tctcaggcaa gcctagttgg tcaatgactg cagccaaact atccgaatca
    32701 gactttaaat cgccataagt gtgttcctgc cccaaaacat tatagacagg atagctaggc
    32761 tgtgtctgag caaaatgctc aatggtttca atcatatctg ctattggttt atttgacaca
    32821 atagggattc tccttcaagt taaaattcat tatagataaa gcttccttga ccctgaccaa
    32881 gatagctaaa gaagtaaagc agccctagaa agataagaaa atacaaggct gtccgaccaa
    32941 gaaagaggta caattctttt ctctgtttca tcaagaaaaa ccattcattt ctgtaatttt
    33001 tcgctaaaat aagagtgatt cttactagct tatttttcta ccattgtacc actttatata
    33061 gtatcttttc aattgtttac cgtatgtttc caatagattt cagcttattt taaggattat
    33121 gcggtttttc tatatctatt tcaaaagaaa aaagcctgag attttcatct caagctctag
    33181 caataatagt tattttctaa actaaaaaag tgagaataca agcacggcca aggctgcacc
    33241 gataacaggt cccacaacag gaatccaagc ataagaccag tctccgtctc ccttgtttga
    33301 aattggaagg atactgtgca tgatacgagg tccaaggtca cgcgcaggat tcaaggcata
    33361 acctgttgtc ccacctagcg atagaccgat accgacaatc aaggtaccca ctgcaaaggt
    33421 tccgatacct gcctgaaaat cgtaaagacc caaagcaaag attgtcaaca ccaaaacaaa
    33481 ggttccaagg atttcgctaa tcaagtttga tacagtatcc ttgatggctg gtccagtact
    33541 gaaggttgcc aggatattgc ctgcattttc ttctgcctca tagtgaggtt tgaattgcaa
    33601 ccaaaccaaa atctgaccca gcatagcccc tgcgaactgg gctaagatat aaggcaaaac
    33661 ggaagcccaa ggcaagtcac cttttaaagc tacaccaatt gtcacagctg gatttaaatg
    33721 agctggactg agcttgccag atacaaatac tgcaaccgca actgcaatcc cccaacccat
    33781 agtaatcaca atccaacctg agctattgct cttggtttta ggaagaacca cacctgcaac
    33841 aacaccattt cctagaagaa tcaggattaa agtccctaga aattctccaa ataattcatt
    33901 catcatcttt ctgtctccat taaaaagaag ggacgagcga caaggaatgc tgccctccac
    33961 ctcttttatt ttttcttaat tttttaattc tgctaaatcg ttgttagcga gagctgcttc
    34021 gacatcagca cggtaagttg ctttttcttc ttctgtccag tcatagaatc gtcccatttc
    34081 atccaaaact ggctcaacga tgctatccaa gctatcacgc ataaagagca tatggttggt
    34141 acgacgaaga aggaagtcaa ctgggctaag agccaactcg ttgcgcattg catagtgaag
    34201 ggacaaagta tctgccaagc tgagtcctgg cgcttgttcc aagctgtgag caagtgcaaa
    34261 gactttcggt gcatttgaac cgtaaagatt tgccagatag tgagcttcct tgctatccaa
    34321 accacgtgat actccaagtt gcgcaaaggc ttcgatttct gaatccacat ttgctgggtt
    34381 caattctcca cctgaaacag ggtaagtttt agaattgatc aatttaaagc tacggtcaaa
    34441 ttctgctttg aggatgtcaa ccacgcgctc catagctcct tcagccatct tacggtagtc
    34501 tgtgatttta ccgccagcaa gagtcaaaag accattgtca tcacggtcca agctagaccc
    34561 acgagaaact gcagatggat ccaaatattt ctcagatgtg ctactttcaa gcttgctgac
    34621 agcagactca acatcttcac gtgttttttc tttggagaga taagattcaa cagtcgcaat
    34681 caagttgtca aagctttcat cactgatggt accgttattt ccaccattat agtcagaggc
    34741 actgttccct gcaatcaatg gacgaagacc tgcccagctg ctttcgatat catcaatggt
    34801 gatgttggat tctggaaagc ggttgttgac aatgccaagt agataatcta catcttcttg
    34861 agttactttt ggatgctcca aatcacctgt gtagtctgta tcagttgtac caaagtaagt
    34921 cttgttttca cgtgggagaa caaagaccat acgaccgtca cccaaacctg tgtcgaagta
    34981 aactggctgt gaaaccttga ttttgcttga atccactacc aagtgaactc ccttagttgg
    35041 gcgcatttgt gagaattgcg ttcccttatt agacaaatta cgtactttat cactccaagg
    35101 acctgttgta ttaataacca gacgggcctt gatttcaaat acttggtctg tcaagagatc
    35161 acgagctaca acacctgtaa tcttgccact ttcgtcaaag aggaagcctt ctgccttcac
    35221 gtggttggca atgagggcac cgtcttggtt ggcacgtttg atgttttcaa tcacgagacg
    35281 cgcatcgttg ttacggaagt caagatacac tccacctcct accaagcctt ccttcttcaa
    35341 gtttggctgg cgttccaaga cttgatcctt gctcaaaacc ttgttcgcag ctgacgtgtt
    35401 gctcacacct gctaaaaggt cgtacaagtc catggctact ttaagacgga agaggctaaa
    35461 ggttgctcca tcttcatcgt aaactggtaa gagcattgga tctgattttg gaatgtgtgg
    35521 agcgatttgt tgaaccactg cacgttcaga aaccgtatct gagaccactt ctacgtcaaa
    35581 ttgtttgagg taacgaagtc ctccgtgaac caattttgtt gaacgactag atgttccttc
    35641 tgcaaagtct tgcatttcaa tcaaaccagt ctcaagaccg ctagctgccg cctgcaaggc
    35701 tacaccagct cctgtgattc ctccaccgat aatcaagagg tccagggtac gttcctgcat
    35761 ttttttaatt gacaattcac gtgttttctt tgaaaattcc atatttacac actttctaaa
    35821 taaatcgctt tattctgcca gtattagtcg tctacttccg caaagacttg agttgctttc
    35881 acagccttct tccagccctt gtagagttgt tccttgcgag attcgttcat agatggctca
    35941 aagagttctc ctgtctcgtt caagagtttc aactcgtcca agtctttcca gtaccctact
    36001 gacaaacctg ctaggaaggc cgctcctaga gctgttgttt ccaggttttt agcacgtgca
    36061 atgtcaatgc ctaaaatatc cgcctggaac tgcatgagga agttgttcat ggctgcacca
    36121 ccatccacct tcagtacttg aatggcggtc tgagtatcca cttgcatggt gtcgatgata
    36181 tcacgcactt gataagcaat agattgcaaa gtcgccttga taaagtcttc tttgcttgtt
    36241 ccacgagtca aaccaaagac ggaaccacga gcattttggt tccagtatgg agcgcctaga
    36301 cctgtaaagg ctggaacgac ataaacttca tcgttgttgt gagaatcacg agcgtatttt
    36361 tcagattctg gtgaattttc aaccatgcga agaccgtcac gaagccactg aatagcactt
    36421 cctgcgatga agatagaacc ttccaaggca taataaacct taccgttgat tccgtaacca
    36481 atggttgtca agaggttgtt ttcagacaac tgcatctctt ccccagtatt catgatgatg
    36541 aaagagcctg ttccataagt attcttaacc atacctggct caaaagccaa ctgtccaaag
    36601 agggctgctt gttggtcccc agccatacct gagattggca cctctccacc gtagaaatgg
    36661 aatggagctg tcttgccgta gatttcggag ttagaacgaa cttctggaag tatagccttc
    36721 ggaatgttaa ggatttccaa aatctcatca tcccatttga gttctttaat gttataaagc
    36781 atggtacgag ctgcatttga gtagtcagtc acgtgagccg caccgtcagt caatttccaa
    36841 accaaccaag tatcgatagt accaaagagc aattcccctt tttctgctcg ctcttgagca
    36901 ccttctacat gatccaaaat ccaacgaacc ttggtagcag agaagtaagc atcaataatc
    36961 aaaccagtct tttcatggaa tttttccaca taaccttggc tttttagttg ctcagccaaa
    37021 ggtgctgtct ggcgtgactg ccaaacgata gcattgtaga taggaagtcc tgttttctta
    37081 tcccagacaa ccgttgtttc acgttggttg gtaatcccga ttgcctcgat ttgatttggc
    37141 ttgacaccac tttcgatgaa agcacccgca ataactgact gaacagagtt ccaaatttca
    37201 ttggcattgt gctcaaccca acctgcctga gggaaaatct gggtaaactc tttttgactc
    37261 gagctaacct tttccccttt tttgttgaaa atgatggcac gagaacttgt agttccctgg
    37321 tcaatggcca tgatgtattt ttcttgtgac atgtactgtc ctcctgataa agatcttgag
    37381 gtgtttcctc tttacactta ctagtataca aaataaagcg ctttcttgtt tatcaacttt
    37441 ttttaatttt taaaaaaatg tgaggagatt gtgtgaattt cacaaaaaag acctggatta
    37501 accaagcctt ttaagtgaat aataactggc gaatcgctac caaatcctcc atatccaagt
    37561 cattttttaa ataatagact ggtgtctgtt ccttcttatg aatcgggtta ttggtaacca
    37621 gcaaatcata atgccgacta gggtcataag cttcaatggt aataaaacga ttgtattcca
    37681 aataccgttt caaaatggct gccatccttg aaaagacaag aaaaccagat gtcaaatcca
    37741 gaccaatccg catatgctgg ccaccgtttt cagccatata ttcgattaac tggagccatt
    37801 cccagagaat cttcttatct aaatctgtcc cctgttgaaa agtaggtaga gcatgaaaaa
    37861 tctcctctgc tgtctcttta aaatcatgtt ccatcagtaa atatggatga cgattctcta
    37921 actggtactt gtagcgatcc tgtaaaatat agcccttata gagatagact tgagcacaaa
    37981 gctgactgag ttcataggtg acatggtcct ctgtataatc ccccaatagt tcctccttct
    38041 tcatttcttg aatcaattgc gtcagtaaat ctgccaactg ccctccaaaa ccaagaatat
    38101 actccatagt atgaagagga agaatgcgat gagataggag aaaagagaag aatatcatca
    38161 tctcaccatc ctcaactcct agtggaatat gttgcccagc aaaaaaggac ggaacctttc
    38221 tcagcaaacg aagataaaag atattgtcaa aataccctcg cattttctct tctatgactt
    38281 gaaactgaca agcattgacc cgaagacgtt gttgactgat gtgagcccag agaaccaagt
    38341 ccaatttctg ccccacagac aaacttgcac cgcagatttc ctctaaattg gcaatctcct
    38401 gttttctctc tggtttctgc atgtgacctt cccattcctg actcgaccag acctttcgga
    38461 aaagacagaa atagaaatag cgaatctgat gctctggacc tcgccaacgt ccattttgga
    38521 tggataaatc aaattctgac agaatctgat ttaaaccagc caagtgacga ccaagcgtag
    38581 cctcgctaat caccaattct tgagccagct gatgggctaa aaactgttgg tggtagagaa
    38641 gataaaccaa aatctggtat ttaacagcac tctccaaaaa taagctccga atatctctcc
    38701 ccttggtagc tgcaccgata gacagacgca aattttcatt ttctgagtga atagtcagct
    38761 ctaagccact atccaaagcc ttgtcattga gcagggtgac atatttggtt agggttgctt
    38821 ttgaaaatcc tgtttcttcc attacagcct tgacggtcgt ctgagactct tgtaatagaa
    38881 aggaaaggat tgaaaattga ccacactcag ctttctccat caaatctcct aaatacattt
    38941 gttacccctt tcattttcta ccttaagcat agcataaatc ttacaaatgc tgaaataatc
    39001 tgtttctctt tttattttca tgctgatttc ctggtccatt atcctgaaaa tcagtaaaca
    39061 cacggctccc cctctgggca tttttatgct aaaatagtag ctatgaataa aattattaaa
    39121 actatatcag aaagcggagc ctttcgtgct tttgtccttg acagcacgga aaccgtccgc
    39181 actgctcaag aaaaacacca aacccaagct agctcaactg tagcgcttgg tcgaactctt
    39241 atcgctagcc agattctcgc agccaatgaa aaaggaaata ccaaacttac agttaaggtg
    39301 ttgggatcta gctctctagg tgctattatc accgtcgctg ataccaaggg gaacgtcaaa
    39361 ggctacgttc aaaatcctgg tgttgacatc aaaaagactg cgactggtga agtcctagtc
    39421 ggaccttttg ttggaaatgg tcaattcctc gttatcacag actacggtac tggaaatcct
    39481 tacaactcta taactcccct catctccgga gaaatcggtg aagaccttgc cttttacctt
    39541 actgaaagcc aacaaacgcc ttcagcggtc ggcctcaatg tccttttgga cgaggaagac
    39601 aaggtcaagg ttgcaggtgg tttcctagtt caagtcttgc caggagccaa gaaagaagag
    39661 attgctcgct ttgaaaaacg catccaagaa atgccagcta tctctactct tctcgaaagc
    39721 gacgaccata tcgaagccct cctcaaggct atctacgggg acgaagccta caagcgtctt
    39781 tctgaagaag aaatccgttt ccaatgtgac tgtagccatg aacgctttat gaacgctctt
    39841 gccagccttc caagctcaga cttacaggaa atgaaagaag aagaccacgg ggcagaaatc
    39901 acttgtcaat tctgccaaac tacttacaac tttgatgaaa aggacctgga ggaactcatt
    39961 cgtgacaaat cttaatacac cttttatgat tggcaatgtt gagattccca atcgtaccgt
    40021 tttagcgcct atggctggcg tgaccaactc agcctttcgt accatcgcaa aagagctcgg
    40081 agctggactc gttgtaatgg aaatggtctc tgacaaagga atccaataca acaacgaaaa
    40141 aaccctgcac atgcttcata tcgatgaggg cgaaaaccct gtctctatcc aactttttgg
    40201 tagcgatgaa gacagcctag cacgcgcagc agaattcatc caagaaaaca ccaagaccga
    40261 tatcgtcgat atcaacatgg gctgccctgt caacaaaatc gtgaagaacg aagctggagc
    40321 tatgtggctc aaggatcctg acaagatcta ctctatcatc aacaaggttc agtctgtcct
    40381 tgatatcccc cttactgtca aaatgcgtac cggatgggcg gacccatctc tggcagtaga
    40441 aaatgccctc gctgctgaag ctgcaggtgt ttctgccctc gccatgcatg gccgtacccg
    40501 tgaacaaatg tatactggcc acgcagacct tgagaccctt tacaaggttg cccaagctct
    40561 aaccaagatt ccattcatcg ccaacggtga tatccgtact gtccaagaag ccaagcaacg
    40621 catcgaagaa gttggtgctg acgcagtcat gattggccga gctgccatgg gaaatcctta
    40681 cctcttcaac caaatcaacc attactttga aacaggagaa atcctacctg atttgacctt
    40741 tgaagacaag atgaagatcg cctacgaaca cttgaaacga ttgattaacc tcaaaggaga
    40801 aaacgtcgcc gttcgtgaat tccgcggcct cgctcctcac tatctccgtg gaacatctgg
    40861 cgctgccaaa ctccgtggag ccatttcaca agctagcacc ctggcagaga ttgaagctct
    40921 cttgcaattg gagaaggctt aatagtttaa aacccgtaac tctcttaaag agtctcttga
    40981 gtgccgccat tctttcctca tcaaaaaaac tccgtagtct ctagcgccat aaatccggct
    41041 agagattaca gagttatttt ttaatctaaa cttcctacaa gggtatagta tctactgaaa
    41101 ggatatggta tttgtaggga atactttaaa gagttcttac aggaagccag cattaacgag
    41161 ttacattagg tttagtttac ccatttacca ttggcattga ctctatagcc acctacagtt
    41221 gtgttgactg caagggcacc tgagccattg acatagtacc atttgtctga tactttgaac
    41281 cattggcttt ctttcatagc ccctgatgct tcaagatagt accatgtatc tccatctttc
    41341 agccaacctg ttgccatcga accgttagca tt
//
LOCUS       DAVLRT010000010        40346 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLRT010000010 DAVLRT010000000
VERSION     DAVLRT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.010"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(63..566)
                     /locus_tag="VXM85_000495"
     CDS             complement(63..566)
                     /locus_tag="VXM85_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345124.1"
                     /translation="MTQFQVRISGEAAYYLRMLKEKYETSEGINITKATILTRAFQNS
                     KTVTNWTKVVQDNSISLKNIYPVEEKELKLNVALSDEVAEGIKKYKEILPSATNTQSV
                     TLGVCVKFLLKAELMTQLNHDISEPEKDFDKKFSILEKQLLEIVAPVNHKLLSDTIWN
                     FKNNFIK"
     gene            995..2272
                     /locus_tag="VXM85_000496"
     CDS             995..2272
                     /locus_tag="VXM85_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1345125.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKIDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFSLNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2348..3832
                     /locus_tag="VXM85_000497"
     CDS             2348..3832
                     /locus_tag="VXM85_000497"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1345126.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGETVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNMQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTTVAAADMQVAVEAYLGL"
     gene            3893..5173
                     /locus_tag="VXM85_000498"
     CDS             3893..5173
                     /locus_tag="VXM85_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1345127.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5177..5797
                     /locus_tag="VXM85_000499"
     CDS             5177..5797
                     /locus_tag="VXM85_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1345128.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLDPEKTY
                     YIGDRTLDVEFAKNSGIQSINFLESSFEGNHRIQALADISRIFETK"
     gene            5963..7141
                     /locus_tag="VXM85_000500"
     CDS             5963..7141
                     /locus_tag="VXM85_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1345129.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASNSEVAETV
                     TAAEEVAPSTGTSVPEEQTAETTRPVEEVAPQATTLAEKQETQVSSQTESAVEATTMP
                     VEEKATETTATSSEAKEVASSNGATAAVSTYQPEETKTISTTYEAPAAPDYAGLAVAK
                     SENAGLQPQTAAFKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIA
                     EYAIQNMASRGISYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG
                     "
     gene            7654..7992
                     /locus_tag="VXM85_000501"
     CDS             7654..7992
                     /locus_tag="VXM85_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1345130.1"
                     /translation="MTRYQILLFLLEHSLCNQMAVQERLKIDQAALTRNFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            8003..8371
                     /locus_tag="VXM85_000502"
     CDS             8003..8371
                     /locus_tag="VXM85_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1345131.1"
                     /translation="MSIITIILTTIVAWEHFYIFYLESIATQSDATSRVFNMEREELA
                     HPSVSLLFKNQGIYKALLGVCLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            8500..9096
                     /locus_tag="VXM85_000503"
     CDS             8500..9096
                     /locus_tag="VXM85_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1345132.1"
                     /translation="MSPFEAIKGLPAEIHGAEVRWLEVPTVFHKSAQVLEEEMNRYQP
                     DFVLCIGQAGGRTSLTPERVAINQDDARTSDNEDNQPIDRPIRPDGASAYFSSLPIKA
                     MVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVKAGFMHIPYMMEQVVNR
                     PTTPAMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(9124..9981)
                     /locus_tag="VXM85_000504"
     CDS             complement(9124..9981)
                     /locus_tag="VXM85_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1345133.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10111..11253
                     /gene="tgt"
                     /locus_tag="VXM85_000505"
     CDS             10111..11253
                     /gene="tgt"
                     /locus_tag="VXM85_000505"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1345134.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11354..13171
                     /locus_tag="VXM85_000506"
     CDS             11354..13171
                     /locus_tag="VXM85_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1345135.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13324..14475
                     /gene="nagA"
                     /locus_tag="VXM85_000507"
     CDS             13324..14475
                     /gene="nagA"
                     /locus_tag="VXM85_000507"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1345136.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14638..15696
                     /locus_tag="VXM85_000508"
     CDS             14638..15696
                     /locus_tag="VXM85_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1345137.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15847..16212
                     /locus_tag="VXM85_000509"
     CDS             15847..16212
                     /locus_tag="VXM85_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1345138.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16288..17229
                     /locus_tag="VXM85_000510"
     CDS             16288..17229
                     /locus_tag="VXM85_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1345139.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17177..18193
                     /locus_tag="VXM85_000511"
     CDS             17177..18193
                     /locus_tag="VXM85_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1345140.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18195..18521
                     /gene="comGC"
                     /locus_tag="VXM85_000512"
     CDS             18195..18521
                     /gene="comGC"
                     /locus_tag="VXM85_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV1345141.1"
                     /translation="MKKMMTFLKKAKVKAFTLIEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18514..18918
                     /locus_tag="VXM85_000513"
     CDS             18514..18918
                     /locus_tag="VXM85_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1345142.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18923..19183
                     /locus_tag="VXM85_000514"
     CDS             18923..19183
                     /locus_tag="VXM85_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345143.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19146..19607
                     /locus_tag="VXM85_000515"
     CDS             19146..19607
                     /locus_tag="VXM85_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1345144.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19585..20031
                     /locus_tag="VXM85_000516"
     CDS             19585..20031
                     /locus_tag="VXM85_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1345145.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            20134..20728
                     /locus_tag="VXM85_000517"
                     /pseudo
     CDS             20134..20728
                     /locus_tag="VXM85_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20789..21742
                     /locus_tag="VXM85_000518"
     CDS             20789..21742
                     /locus_tag="VXM85_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1345146.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFTEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21793..22983
                     /locus_tag="VXM85_000519"
     CDS             21793..22983
                     /locus_tag="VXM85_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1345147.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADVIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23132..23503
                     /gene="rnpA"
                     /locus_tag="VXM85_000520"
     CDS             23132..23503
                     /gene="rnpA"
                     /locus_tag="VXM85_000520"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1345148.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23478..24302
                     /locus_tag="VXM85_000521"
     CDS             23478..24302
                     /locus_tag="VXM85_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1345149.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24321..25307
                     /locus_tag="VXM85_000522"
     CDS             24321..25307
                     /locus_tag="VXM85_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1345150.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25358..25981
                     /locus_tag="VXM85_000523"
     CDS             25358..25981
                     /locus_tag="VXM85_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1345151.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26112..27569
                     /locus_tag="VXM85_000524"
     CDS             26112..27569
                     /locus_tag="VXM85_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1345152.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27592..27873
                     /locus_tag="VXM85_000525"
     CDS             27592..27873
                     /locus_tag="VXM85_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1345153.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27950..28435
                     /locus_tag="VXM85_000526"
     CDS             27950..28435
                     /locus_tag="VXM85_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1345154.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28452..29117
                     /locus_tag="VXM85_000527"
     CDS             28452..29117
                     /locus_tag="VXM85_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1345155.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDIFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29121..29984
                     /locus_tag="VXM85_000528"
     CDS             29121..29984
                     /locus_tag="VXM85_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1345156.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29986..30690
                     /locus_tag="VXM85_000529"
     CDS             29986..30690
                     /locus_tag="VXM85_000529"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1345157.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKTWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30869..32542
                     /locus_tag="VXM85_000530"
     CDS             30869..32542
                     /locus_tag="VXM85_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1345158.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32654..33745
                     /gene="ulaG"
                     /locus_tag="VXM85_000531"
     CDS             32654..33745
                     /gene="ulaG"
                     /locus_tag="VXM85_000531"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1345159.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33859..35835
                     /gene="tkt"
                     /locus_tag="VXM85_000532"
     CDS             33859..35835
                     /gene="tkt"
                     /locus_tag="VXM85_000532"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1345160.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGINTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35952..36251
                     /gene="yajC"
                     /locus_tag="VXM85_000533"
     CDS             35952..36251
                     /gene="yajC"
                     /locus_tag="VXM85_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1345161.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36298..36726
                     /locus_tag="VXM85_000534"
     CDS             36298..36726
                     /locus_tag="VXM85_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1345162.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36728..37138
                     /locus_tag="VXM85_000535"
     CDS             36728..37138
                     /locus_tag="VXM85_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345163.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37438..40089
                     /gene="adhE"
                     /locus_tag="VXM85_000536"
                     /gene_synonym="adhC"
     CDS             37438..40089
                     /gene="adhE"
                     /locus_tag="VXM85_000536"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1345164.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSVHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFRPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12327 a         7160 c         8973 g        11886 t
ORIGIN      
        1 tgagtatcag ataactcgtt ttgttcgctt tttatattta agattagact tttgttccag
       61 actcatttta taaaattatt tttgaaattc catatggtat cagaaagtaa tttgtggttg
      121 actggtgcaa cgatttcaag aagctgtttt tctagaatag agaatttctt gtcaaaatct
      181 ttttcaggtt ctgaaatatc gtgatttaat tgggtcatca attctgcttt aagaagaaat
      241 ttaacacata cacctaaagt tacactttga gtatttgtgg cactaggcaa tatttcttta
      301 tattttttaa ttccttctgc tacttcatcg gacagtgcaa cgttcaattt taattccttt
      361 tcttccactg gataaatatt ttttaaagag atagagttat cttgtacaac ttttgtccag
      421 tttgttacag tctttgaatt ttgaaacgca cgcgttaaaa tggtagcttt agttatattg
      481 ataccttctg aagtttcata tttctctttt agcattctta aatagtaagc tgcttcacca
      541 gatattcgga cttgaaattg tgtcattatt agtacctccg tattgtatta taccatttgt
      601 acattaaaag gacaatgtat aaacgaataa tctaccttct cgttcaagca tttttacaag
      661 tttagtaatc ttgttgaaga tgcattccat gtgttacgtt taaaaaaggg aattaaagta
      721 atctagttcc taaaaggtta ctaaaaataa aaaaactatc gtttttgtga tagaggtaac
      781 tttttgaaaa ggctatttga ctggctttat atacttatga aatgttaaga gcccaactca
      841 aaatccagtg tccgcaagga cgtgccggtt cgaccccggc cgccggtata gtatagtgtt
      901 aggaacgttg ttattcttcg ttcctttttt atattatttt tggtataatt atagttattc
      961 aaattttatt tagattaaga aagtgtaggg gagtatgtct tgttctatcg atttattaaa
     1021 acatcggtat ttgaaaaata ttaaagaaaa tcctgaattg tttgtcggaa ttgagttgga
     1081 gtatcctgtt gcaagtttag aaggggatgc tacagatgtt gaagttataa aggatctatt
     1141 tcattattta gtttctactt tggatctcac cgtagcaaag atagatgatt ttggcaatct
     1201 gatccagtta gtagatccga taagtcagga tgctatttta tttgaagttt cctatacaac
     1261 gattgagttt gcatttggta aggctgaaac gattcaagag gtcgaaaatc gtttcaataa
     1321 ttatatgaat gtaattcaga gaaagttagc tgaatcaaat catgctattg ttggctgtgg
     1381 tatccatccc aactgggata aaaatgagaa ttgtccagtg gcttatccac gctatcagat
     1441 gttgatggat tatttgaatt tgagtagaaa tattattaaa tcagatttac atcatttccc
     1501 tgaatatggt acttttatct gtgggagcca ggttcagctg gatatttcaa aaaccaacta
     1561 cttacgggtg attaatgctt ttactcaaat tgaagcggct aaggcttatt tatttgcaaa
     1621 ctctgagttt tcgggtgcgg attgggatac gaaaatttca agggatattt tctgggaaga
     1681 atctatgcat ggtatctatc cagagaatgt tggggtcaat gctagactcc ttaatgatga
     1741 agctgatttt tttgactatc taaatcattc tgcgattttt actgcggaac gtgatgggca
     1801 gacctattat ttttatccta ttcaggctgg ggactatttg gctacgcctg aaatccaagc
     1861 attttctctg aatggggatg aggttattat ttacccccaa gagaaggatt ttgaaactca
     1921 tcgtagttac cagtaccaag acttaacgac tcgaggaaca gttgagtttc gtagtgtgtg
     1981 tacacagcca cttgatagaa cttttgcttc tgcagctttt cacttgggat tattggttaa
     2041 tttagacaag ttagaagctt acttagaaac agcacctttc tttaaagtat ttggttatga
     2101 ttacaagtct ttaaggagac aattttctaa gaaaaatctt acagatgagg aagaaactac
     2161 gattattgaa ttttccaaag acttactcct actagctgag gagggactag tggtgagaaa
     2221 taaggaagaa atgacctatt tacagccttt gagagaagaa ttgagcctat aatttctctt
     2281 ataaagggag aattttctga aaaatcatga tataatggac gagactatag ataaaggata
     2341 gagagtaatg acattagttt atcaatcaac gcgtgatgcc aacaatacag taactgccag
     2401 tcaagcaatt ttgcaaggtt tggcgacgga cggcggtttg tttacaccgg ttacttatcc
     2461 aaaggtagat ttggactttg acaaattgaa agatgcttct taccaggaag ttgctaagct
     2521 agttttgtca gcatttttag atgactttac agctgaggag ttggactact gtatcaacaa
     2581 tgcctacgat agcaaatttg atactccagc tattgcacca ttagtgaaat tagatgggca
     2641 atacaatttg gaacttttcc atggttcaac gattgccttt aaggatatgg ccttgtctat
     2701 tttgccatac tttatgacga ctgctgctaa gaaacatggt ttggagaaca agattgttat
     2761 cttgacagcg acatctggtg acacggggaa agctgctatg gcggggtttg cggatgtgcc
     2821 tggtactgag attatcgtct tttatccaaa ggatggtgtc agcaagattc aagagttaca
     2881 aatgaccact cagactggcg acaatactca tgttattgct attgatggta actttgacga
     2941 tgcgcaaaca aatgtgaagc acatgtttaa cgatgtggct cttcgtgaaa aattgactac
     3001 caacaagttg caattttcat cagctaactc tatgaacatt ggtcgtctgg tgccacaaat
     3061 tgtttattat gtttatgctt acgctcaatt ggttaagact ggtgaaactg tagctggtga
     3121 aaaggttaac ttcacagtac caacaggaaa ctttggaaat atcttggctg ccttttatgc
     3181 caaacaaatt ggtttgccag ttggtaaatt gatctgtgct tcaaatgaca acaatgtttt
     3241 gacagacttc tttaaaacac gtgtctatga caaaaaacgt gagtttaagg taacaaccag
     3301 cccatctatg gatatcttgg tatcttcaaa cttggagcgc ttgattttcc atcttttggg
     3361 aaataatgct gaaaagacag ctgaactcat gaatgctttg aacatgcaag gacaatataa
     3421 gttgacagac tttgatgcag agattttgga cctctttgca gctgaatatg cgactgagga
     3481 agaaacagcg gcagaaatca agcgtgttta tgagttagat tcttatattg aggatccaca
     3541 tacggcggtt gcctcagcag tttatagaaa ataccaagcg gttactgatg atgcgactaa
     3601 gacagtgatt gcttcaacag ctagtcccta caagttccca gtagttgcag tagaagctgt
     3661 aactggaaaa gcaggtttaa cagactttga agctttggct caattacatg aaatctcagg
     3721 cgttgcagtg ccaccagcag ttgatgggct tgagacagct cctgttcgtc acaagacaac
     3781 agtggcagct gctgacatgc aagtagcggt tgaggcttat ttaggacttt aagatagagg
     3841 gagtaaactc ggttgggaaa ccaactgagt ttcttttcat caggaggaga gattgtttaa
     3901 gaaaaataaa gacattctta atattgcatt gccagctatg ggtgaaaact ttttgcagat
     3961 gctaatggga atggtggaca gttatttggt tgctcattta ggattgatag ctatttcagg
     4021 ggtttcggta gctggtaata ttatcaccat ttatcaggcg attttcatcg ctctgggagc
     4081 tgctatttcc agtgttattt caaaaagcat agggcagaaa gaccagtcga agttggccta
     4141 tcatgtgact gaggcgttga agattacctt actattaagt ttccttttag gatttttgtc
     4201 catcttcgct gggaaagaga tgataggact tttggggacg gagagggatg tagctgagag
     4261 tggtggactg tatctatctt tggtaggcgg atcgattgtt ctcttaggtt taatgactag
     4321 tctgggagcc ttgattcgtg caacgcataa tccacgtctg cctctctatg ttagtttttt
     4381 atccaatgcc ttgaatattc ttttttcaag tctagctatt tttgttctgg atatggggat
     4441 agctggtgtt gcttggggaa caattctgtc tcgtttggtt ggtcttgtga ttttgtggtc
     4501 acaattaaaa ctgccttatg ggaagccaac ttttggttta gataaggaac tgttgacctt
     4561 ggctttacca gcagctggag agcgacttat gatgagggct ggagatgtag tgatcattgc
     4621 cttggtcgtt tcttttggga cggaggcagt tgctgggaat gcaatcggag aagtcttgac
     4681 ccagtttaac tatatgcctg cctttggtgt cgctacggca acggtcatgc tgttggcccg
     4741 agcagttgga gaggatgatt ggaaaagagt tgctagtttg agtaaacaaa ccttttggct
     4801 ttctctgttc ctcatgttgc ccctgtcctt tagtatatat gtcttgggtg taccattaac
     4861 tcatctctat acgactgatt ctctagcggt ggaggctagt gttctagtga cactgttttc
     4921 actacttggg acccctatga cgacaggaac agtcatctat acggcagtct ggcagggatt
     4981 aggaaatgca cgcctccctt tttatgcgac aagtataggc atgtggtgta tccgcattgg
     5041 gacaggatat ctgatgggga ttgtgcttgg ttggggcttg cctggtattt gggcagggtc
     5101 tctcttggat aatggttttc gctggttatt tctacgctat cgttaccagc gctatatgag
     5161 cttgaaagga taggaaatgc aaaaaacagc ttttatttgg gatttagacg ggactttatt
     5221 ggactcttac gaagcgattt tatcagggat tgaggagact tttgctcagt tttctattcc
     5281 ttatgataag gagaaggtga gagagtttat cttcaagtat tcggtgcaaa atttgcttgt
     5341 gcgggtggca gaagatagaa atctggatgt tgaggtgcta aatcaggtgc gtgcccagag
     5401 tctggctgag aagaatgctc aggtagtttt gatgccaggt gcgcgtgagg tgctagcttg
     5461 ggcagacgaa tcaggaattc agcagtttat atatactcat aaggggaaca acgcttttac
     5521 cattctcaag gacttggggg tggaatccta ttttacagag attttaacca gtcagagtgg
     5581 ctttgtgcgg aagccaagtc cagaagcggc tacctatctg ctagataagt atcagctgga
     5641 tcctgagaag acctattata taggggaccg gactttagat gtggaatttg ccaagaatag
     5701 cggtattcaa agcatcaact ttttagaatc ttcttttgaa ggcaatcaca ggattcaagc
     5761 actagcagat atttcccgta tttttgagac taagtgataa aaagattgtg tcagttttgt
     5821 gacagagacc taacaaacta tttcaagtaa tctagtttgt tacaaggaat agacagttct
     5881 gttaaatagg cccgagaggg ctttttttct acattttttg tgttatgata gacaggtact
     5941 catttgaaag gaatttgaaa gaatgaagaa aagaatgtta ttagcgtcaa cagtagcctt
     6001 gtcatttgcc ccagtattgg caactcaagc agaagaagtt ctttggactg cacgtagtgt
     6061 tgagcaaatc caaaacgatt tgactaaaac ggacaacaaa acaagttata ccgtacagta
     6121 tggtgatact ttgagcacca ttgcagaagc cttgggtgta gatgtcacag tgcttgcgaa
     6181 tctgaacaaa atcactaata tggacttgat tttcccagaa actgttttga caacgactgt
     6241 caatgaagca gaagaagtaa cagaagttga aatccaaaca cctcaagcag actctagtga
     6301 agaagtgaca actgcgacag cagatttgac aaccaatcaa gtgaccgttg atgatcaaac
     6361 tgttcaagtt gcagatcttt ctcaaccaat tgcagaagct ccaaaagaag tagcatcaaa
     6421 ttcagaagtt gcagagacag tgactgctgc agaagaagtg gcaccatcta cgggcacttc
     6481 tgtcccagag gagcaaacgg ccgaaacaac tcgcccagtt gaagaagtag ctcctcaggc
     6541 aacgactcta gctgagaagc aggaaacaca agtgagctct caaaccgagt cagcagtgga
     6601 agcaactaca atgccagtag aagaaaaagc aaccgaaaca actgcaacaa gttcagaagc
     6661 aaaagaagta gcatcatcaa atggagctac agcagcagtt tctacttatc aaccagaaga
     6721 gacgaaaaca atttcaacaa cttacgaggc tccagctgcg cccgattatg ctggactggc
     6781 agtagcgaaa tctgaaaatg caggtcttca accacaaaca gctgccttta aagaagaaat
     6841 tgctaacttg tttggcatta catcctttag tggttatcgt ccaggagaca gtggagatca
     6901 cggaaaaggt ttggctatcg actttatggt accagaaagt tcagaattag gggataagat
     6961 tgcggaatat gctattcaaa acatggccag ccgtggcatt agttacatca tctggaaaca
     7021 acgtttctat gctccattcg atagcaaata tgggccagct aacacttgga acccaatgcc
     7081 agaccgtggt agtgtgacag aaaatcacta tgaccacgtt cacgtttcaa tgaatggata
     7141 aacccgactt ggtaacatca ttttgacgaa tgagatctag ctttcgtgat aggaagcgat
     7201 tctcgttcgt tttttctttg tcatactctt cgaaaatctc ttcaaacaac gtcagtttta
     7261 tctgaaacct caaagctgtg ctttgagcaa cctgcgacta gcttcctagt ttgctttttg
     7321 attttcattg agtatcaatt tgaatggaaa atggaaagtt atcatcttgt aatgagttaa
     7381 gcaacattct tgcaatctat tttatagtag attgaaacta gaatagtaca caactgcttc
     7441 taaaacattg ttagaaatcg atttgactgt cctgatcgat ttgtcctgct cttatttcat
     7501 ttcactatac tttatattat aattgactgg tcaaatattg ataaatcaat aaaaagagag
     7561 gggaagaaat gctagagatt caagatttac tgtatcaact ccgcttgtct gagcaagtga
     7621 gtacgcaatt gtttgaaaaa gcttgggatt agtttgacac ggtatcagat tttactgttt
     7681 ttgctggagc attctctttg taaccaaatg gcggttcagg agcgtttgaa aattgatcag
     7741 gctgctttga cacggaattt caagattttg gaaacggaag gtttggtgga gcgtcatcgt
     7801 aatcctgaaa atcagcggga agtattggta gaggctgcga agtatgccaa ggagcagtta
     7861 gtggtgaatc cccctctgca acatatcaag gttaaggaag agatggaaag tatcttaaca
     7921 gagtttgaga gaacagaact cagccgttta ttaaataaat tggttttggg tattgaaaat
     7981 atagaaattt aaggagaaat agatgtcaat tattacaatt attttaacaa cgatcgttgc
     8041 ttgggagcat ttttacattt tttatttaga aagtattgcc acgcaatcag atgcgactag
     8101 tcgtgtattt aatatggaaa gggaagaatt ggctcatccg tcagtaagtt tattgttcaa
     8161 aaatcaaggg atttataagg ctctgctagg agtctgtctc ttgtatggca tttatttctc
     8221 acagaattta gaaattgtga ctatttttgt cttatttgtg attggtgctg cgacttacgg
     8281 ctctttaaca gcggataaaa aaattatttt gaaacaaggt ggatcagcta ttttggcctt
     8341 gattagtatt ttactcttta aatacacttg aaggtcgatt ctaatctcgc taatcctttt
     8401 taatccagaa taagggaaat atgttatact tgtttttaag aaaaaagttt cattgaattg
     8461 gttttgagga gttagaaatg aaagtattag tgacaggttt tgagcccttt tgaggccatt
     8521 aaaggtttac cagctgaaat ccatggtgct gaggtccgtt ggctagaggt gccgacagtt
     8581 tttcacaaat ctgctcaagt attggaagaa gagatgaatc gttatcaacc tgactttgtc
     8641 ctttgtattg ggcaagctgg tggaagaact agtttgacac ctgaacgagt ggccattaat
     8701 caagacgatg cacgtacttc tgataacgaa gataatcaac cgattgaccg tcccattcgc
     8761 ccagatggtg cttcggccta ctttagtagt ttgccgatta aagcgatggt tcaagctata
     8821 aaaaaagagg gcttaccggc ctctgtttcc aatacggcag ggacttttgt ctgcagccat
     8881 ttgatgtatc aggctctcta tttggtagaa aagaaatttc catatgttaa ggcaggtttt
     8941 atgcatattc cttatatgat ggaacaggtg gtgaacagac cgactactcc agctatgagt
     9001 ttagtggata ttcggcgagg gatagaagca gcaatcggcg ctatgataga acatggagat
     9061 caggaactca agttggtagg cggagaaact cattgataga aaaaagcttg aggggaaaaa
     9121 ccttcaagct tttggacgtt ttcgagccaa tactgctcgg taaaacataa ttttagtgca
     9181 ttggatataa ggtaggagtg aaaaactagc aatgccaaag gtaatccaat tgaggaagta
     9241 ccaaggaaga agctgtaaat ctaggacaaa gcgctggaac ttgtagccct tcataaagga
     9301 acggctagtt tttaggattc gtcttggtgg gacctgtcct aggtctagac tataacagag
     9361 aagaaattcc acctgtgaat aggcataata ctgtggaata tagaggatat ttcctacaat
     9421 gatcaagatg agacttgcaa gaaagtagag tccaaagacc atgaggaaac gctcggtttc
     9481 aactgatgag agatctagat ttggaaactc aggatgtagg gtgacgaatt ttttggctaa
     9541 aaagctacta taaaagagga ggtaaatccc aagtaaatta gggatactcc ataaaaagag
     9601 atagaaacgt ttgagaagta gggtcaaaaa ggtttgagaa aagcgctcct catcaaagag
     9661 agctaggctg ttttttacag atggctccgt tttagaatct ttcatgagtg tcagtgttgc
     9721 atagacggaa ctggtcaaaa gaatagtccc gataaaggag actagtagag gaaagaggta
     9781 ggtttgaagt atttggccaa gtatgctgaa aaatggctgt tctaaaacag tcccgtggat
     9841 ccgagataag ggattaagaa aactagataa gatgaccagc atactgggaa ggatatagag
     9901 gagaaagaga cggggggtgt cagcctgaaa atgttttgac tcctgacgaa ttgtttttaa
     9961 atcaattttt ggatagttca ttctcttatt ataccatagt tcttatacat agttcgtgac
    10021 agttcctact ttttttgata aaatcataca gtgtgtcctt gggcacactg tatgaactgg
    10081 gactgtcttt cccagcttcg gaggtaaaaa atgtcagatt caccaatcaa atatcgtttg
    10141 attaagaaag aaaaacacac aggagctcgt ctaggagaaa tcatcactcc ccacggtacc
    10201 tttccgacac ctatgtttat gccagttggg acacaagcca ctgtcaaaac tcagtcacct
    10261 gaagaattga aggagatggg ttcgggaatt atcctatcaa acacctatca tctctggctt
    10321 cgccctggag atgaactcat tgcacgcgct ggtggtctcc acaagttcat gaattgggac
    10381 cagcctatct tgacagatag tggtggtttt caggtttatt ctttagcaga tagccgtaat
    10441 atcacagaag aaggagtaac ctttaaaaat catctaaatg gttctaagat gctcctatcc
    10501 ccagaaaaag ccatctctat tcagaataat ctgggttcag acatcatgat gtcctttgat
    10561 gaatgtcctc agttttatca accttatgac tacgttaaga aatcgatcga gcgtaccagc
    10621 cgttgggctg agcgtggttt gaaggctcac cgtcgtccac atgaccaagg tttgtttgga
    10681 attgtgcaag gtgcaggatt tgaagacctt cgccgccaat cagctcatga tcttgtcagc
    10741 atggatttct caggctactc tatcggtggt ttggcagtgg gagaaaccca tgaagagatg
    10801 aatgcggtct tggactttac aactcaactg ctgcctgaaa ataaacctcg ttatctgatg
    10861 ggtgtgggag cgccagatag cttgatcgat ggggtcattc gtggggtgga tatgtttgac
    10921 tgtgtcttac cgactcgaat tgctcgtaac gggacttgta tgaccagtca aggacgtttg
    10981 gttgtgaaaa atgcccagtt tgctgaggac tttacgccac tggatcctga gtgtgattgc
    11041 tacacatgta ataactatac acgcgcttac cttcgtcacc tgctcaaggc tgatgaaacc
    11101 tttggtatcc gcttgactag ctaccacaat ctttacttct tgcttaacct gatgaagcaa
    11161 gtgcgacaag ccatcatgga tgacaatctc ttggaattcc gtaagtattt tgtggaaaaa
    11221 tatggctata ataagtcagg acgtaatttc taaaatggaa ttgatataag ctaaaaatcc
    11281 taagttttct cttaggattt ttcttctttt tttgatagaa taaagtgtac aatgaaagga
    11341 agaataaact cgtatgcgca ttaaatggtt ttccttggtt aggattacag gtttactttt
    11401 ggtactcttg tatcacttct ttcagacgat ctttcctgga ggatttttcg gggtagatgt
    11461 ctttttcaca ttttcaggtt tcctgattac agctctactc attgaagaat tttctaaaaa
    11521 ccatgagatt gatttgatag gattttttag aagacgcttt tatcggattg tgccacctgt
    11581 ggttttgatg gtcttggtga ccatgccttt tactttcttg gttcgccaag actatgttgc
    11641 tggaattggt ggccagattg cgagcgtctt aggctttatg accaacttct atgaactcct
    11701 aacaggtggg agttatgaat ctcagttcat tcctcatttg tttgttcata attggagttt
    11761 ggcagttgag gttcactact atattctttg gggattggca gtttggttct tatccaaaca
    11821 agctaaatca aatggtcagt tgaaggggat ggtctttctc ttatctgctg ttgccttctt
    11881 gatcagtttc ttctccatgt ttattggtag ttttctagtg acctcttatt cctctgttta
    11941 tttctccagt ttaactcatg tctatccatt ctttttggga agtatgttag caactattgt
    12001 aggcgttcgt cagacgactt ccctcgtcaa gcagttggat aaaatctggg atttacgaaa
    12061 gactttggta gtttttggag gaggctttgg tttcctagtt cttttgactt tctttgtcaa
    12121 attcacttat ctttttgcct atcttatcgg cttcttactt gccagtcttg cagctcttgc
    12181 catgattctg gcggcgcgtg tcttacatga aaagacacat catatacagg agccgaagat
    12241 tatcagcttt ttagcggata ctagctatgc ggtttatctt ttccattggc ctttctatat
    12301 cattttctca cagttgacat caaatcttct tgctgtatta ctgactctga tttgttctta
    12361 tggctttgcc agtctgtcat tctatgtatt ggaaccttgg attgcaggca agaacacacc
    12421 tattgtccaa acccttcgtc ccctgcctta tattcacgca attcttgcag caggtacagg
    12481 aatcttgacc atcattgtct gcacggtgac cttgttggca ccacaagtgg gagcgtttga
    12541 gacagacttg actgtcaatg gcttgaagca agctgcaaca aatattggcc agaccaaggt
    12601 gatggcagaa cgggcagatg caaacagttt gggaattgct gatggcacta tgttaattgg
    12661 tgactcagtg gctttaaggg caaatacagc actacagaca gctcttcctg gagcacagat
    12721 taacgcgcag gtcagcgtaa caaccaagac cgcaaatgaa atcatgctaa ataatagcca
    12781 gaataaattt ttacctaaga cggtggtcat tgcgactggg gtaaataatc ctgagaatta
    12841 caaggatgac tgggacagta tcgtgaaaaa tcttcctaag ggacaccata tgattttggt
    12901 gactccttat gagggagata agacaaaaga gacctatgcc atcgttgaga aggctgctgc
    12961 ctatatgaga gaattggcag agaagacacc ttacattacg atagcagatt ggaatcaagt
    13021 tgcgaaagag catccagaaa tttgggcagg aacagaccag gttcatttcg ggagtgagag
    13081 tagcactatc gaagcaggag caaaattgta tgcagatacg attgccacag ctttgcagac
    13141 agctcaagac aagccggtta aatcaaaata acttgtatta aaaaagagaa gagttggaga
    13201 aatccagctc ttttaaaata tctccaaaaa ataggtctat accatttaca aataaaaaag
    13261 aaaggtttat aatgtaattg acataataaa ttgtagaatc aatcttttaa ggaggttaac
    13321 attatgccta actatattaa agcggatcag tttttctacc cacacggagt tcgtcgaggt
    13381 ggttacttgg aacttgtgga cggcaagttt gggaaacatg tagagcagat tcctgaaggg
    13441 gctgaggtga ttgactatac aggttatagc attgccccag gtcttgtgga tactcatatt
    13501 catggatatg caggtgtaga tgtgatggac aacaacattg aaggtacatt gcatactatg
    13561 agtgaaggac ttcttagtac cggtgttacc agtttcttac ccacaacttt aacagccact
    13621 tatgagcaat tgcttgcagt cactgaaaat cttggaaacc attataaaga agcaacaggt
    13681 gctaagattc gtgggattta ttatgaaggt ccatatttca cagaaacttt taagggggca
    13741 caaaatccaa cttatatgag agacccgggt gttgaggagt ttcattcttg gcaaaaagcg
    13801 gcaaatggct tgcttaataa aattgccctt gcaccagaac gtgatggggt ggaggacttt
    13861 gttcgtacag ttacgggcga aggtgtgacg gttgctcttg gacattcaaa cgcgactttt
    13921 gatgaagcca aaaaagcagt cgatgctgga gcgagtgttt gggtgcatgc ctacaatgga
    13981 atgcgtgggt tgactcaccg tgaattgggt atggttggag ccatgtacca attgccacat
    14041 acctatgcag agttgatctg tgatggccac cacgtagatc caaaggcttg cgaaattctt
    14101 atcaaacaaa aaggaacaga aaacatcgct cttatcacag actgtatgac agctggggga
    14161 ttggaagacg gagattatat gttgggagaa ttcccagttg tcgttgcaaa tggaactgca
    14221 cgcctcaaat cgacaggtaa cttggcaggt tctatcctca aactcaaaga tggtttgaag
    14281 aatgtggtcg aatggggaat tgcgaatccg catgaagcag tcatgatggc cagcttcaac
    14341 ccagctaaat ccgttcacat cgatgatgtc tgtggccaaa tccgtgaagg ctacgacgct
    14401 gacttcatcg tattagataa agatttggaa ttggtagcaa cctacctaga tggcgtaaaa
    14461 cgttatcaag cataagagag aaagcagaag ttagagacta acttctgctt ttttatgcag
    14521 ggtactttac tggtaaataa aaagttaaaa aaatcacaaa aaaagcttga agaaacaaag
    14581 aaaaaggctt acaattgaac tataaatatt acaaataaaa aaacggagga gtgctttatg
    14641 aaagcctata cttatgttaa accaggactt gcttcttttg ttgatgtaga caaaccagtt
    14701 attcgcaagc caacagatgc tattgtgcgt attgtaaaaa ccactatttg tggaacagac
    14761 ctccatatta tcaaagggga tgttcctact tgccaaagtg gtaccattct tggccacgaa
    14821 gggattggga ttgttgaaga agttggggaa ggagtttcca acttcaaaaa aggtgacaag
    14881 gtcttgattt cttgcgtctg tgcctgtggt aaatgctact actgtaaaaa aggaatttat
    14941 gctcactgtg aagacgaagg gggctggatt ttcggtcact tgattgatgg tatgcaggct
    15001 gaatatctac gtgtccctca tgcagataat actctttacc atactccaga agacttgtca
    15061 gatgaagctt tggttatgct gtcagacatt ctgcctactg gatatgaaat tggtgtctta
    15121 aaagggaaag tagaacctgg ttgcagcgta gccattattg gttcaggtcc agttggattg
    15181 gctgctcttt taacagccca attctattca ccagctaaat tgattatggt agacctagac
    15241 gataaccgct tggaaactgc cctatcattc ggtgcgactc ataaggttaa ttcttcagac
    15301 cctgaaaaag ccattaaaga aatttatgat ttgacagatg gtcgtggtgt ggatgtcgct
    15361 atcgaagctg ttggtattcc tgcaacattt gatttctgtc aaaagattat cggtgtagac
    15421 ggaacggttg ccaactgtgg tgtgcatggt aaaccagttg aattcgattt agataaactt
    15481 tggattcgca acatcaatgt aacaactggt ttggtatcta caaatacgac tccacaattg
    15541 ttgaaagcac ttgaaagtca taagattgaa ccagaaaaat tggtaactca ctatttcaaa
    15601 ctcagtgaaa ttgaaaaagc ctatgaagtc ttcagtaagg cagcagacca ccatgccatt
    15661 aaggtcatta tcgaaaacga tatctcagaa gcctaagtag taaaaatatt tttgtacata
    15721 agtaaataga aattcagtca tccatcagat ggctggattt tttatcaaaa aattaagaaa
    15781 tgagcatatt tctttccttg tctggcggaa ttggttataa tatacggtac aaaggaatga
    15841 atgaatatgt atcgtgttat agaaatgtac ggagattttg aaccgtggtg gttcttagaa
    15901 ggttgggaag aagatattgt agcaagtaga aaatttgacc agtattatga tgctctcaaa
    15961 tactacaaaa cttgctggtt tagattggaa caagaatccc ctctttataa aagtagaagc
    16021 gacttgatga ccattttttg ggacccggaa gaccaacgct ggtgtgatga atgtgatgag
    16081 tatttacaac aataccattc tttggctctt ttgcaggatg agcaggttat cccagacgaa
    16141 aaactacgct caggctatga aaaacaaacc agtcaggaaa ggaatcgttc ttgccgtatg
    16201 aaattaaaat agagaaaagt aacttttttg gagttgcttt ttttattttt ctaacgcttt
    16261 gcgaatagta taggtgagga ggtaagtatg gttcaagaaa ttgcacaaga aatcattcgt
    16321 tcagctcgga aaaaagggac gcaggatatt tattttgtcc ctaagttaga cgcctatgag
    16381 cttcatatga gggtaggaga cgagcgctgt aaaattggta gctatgattt tgaaaagttt
    16441 gcagccgtta tcagtcactt taagtttgtg gcgggtatga atgtgggaga aaaaagacgt
    16501 agtcaactgg gttcctgtga ttatgcctat gaccagaaga tggcgtctct acgtttatct
    16561 actgtaggcg attatcgggg acatgagagt ttggttatcc gtttgttgca cgatgaggag
    16621 caggacttgc atttttggtt tcaggatatt gaagaattag gcaagcagta caggcaacgg
    16681 ggactctatc tttttgctgg cccggttggg agtggtaaga cgaccttgat gcatgaattg
    16741 tccaagtcac tctttaaagg acagcaagtt atgtccatcg aagatcctgt cgaaatcaag
    16801 caggacgaca tgcttcagtt gcagttgaac gaagcaatcg gcctaaccta tgaaaatcta
    16861 atcaaacttt ccttgcgtca tcgaccagat ctcttgatta tcggagaaat tcgtgacatc
    16921 gagacggcgc gtgcagtggt cagagctagt ttgacaggtg cgacagtctt ttcaaccatt
    16981 cacgccaaga gtatccgagg tgtttatgag cgtctgctgg agttgggtgt gagtgaagaa
    17041 gaattggcag ttgttctgca aggagtctgc taccagagat taatcggggg aggaggaatc
    17101 gttgactttg caaacagaga ttatcaagaa caccaagcag ccaagtggaa tgagcaaatt
    17161 gaccagcttc ttaaagatgg acatatcaca agtcttcagg ctgagacgga aaaaattagc
    17221 tacagctaag caaaaaaata tcatcaccct atttaacaat ctcttttcta gcggttttca
    17281 tctggtggag actatctcct ttttagatag gagttccttg ttggacaagc agtgtgtgac
    17341 ccagatgcgt acaggcttgt ctcaggggaa atcattctca gaaatgatgg aaagtttggg
    17401 atgttcaagt accattgtca ctcagttatc tctagctgaa gttcatggca atcttcacct
    17461 gagtttggga aagatagaag aatatctaga caatctggcc aaggtcaaga aaaagttaat
    17521 tgaagtagcg acctatcctt tgattttgct gggttttctt ctcttaatta tgctggggct
    17581 acggaattac ctgctcccac aactggatag tagcaatatt gccacccgaa ttatcggtaa
    17641 tctgccacaa atttttctag gcatggtagg gcttgtttcc gtgcttgccc ttttagcact
    17701 aactttttat aaaagaagtt ctaagatgag tgtcttttct atcttagcac gccttccctt
    17761 tattggaatc tttgtgcaga cctacttgac agcctattat gcacgtgaat gggggaatat
    17821 gatttcacag ggaatggagc tgacgcagat ttttcaaatg atgcaggaac aaggttccca
    17881 gctctttaaa gaaatcggtc aagatctggc tcaaaccctg aaaaatggcc gtgaattttc
    17941 tcagacgata ggaacctatc ctttctttag gaaggaattg agtctcatca tagagtatgg
    18001 ggaagttaag tccaagctgg gtagtgagtt ggaaatctat gctgaaaaaa cttgggaagc
    18061 cttttttacc cgagtcaacc gtaccatgaa tttggtgcag ccactggttt ttatctttgt
    18121 ggcactgatt atcgttttac tttatgcggc aatgctcatg cccatgtatc aaaatatgga
    18181 ggtaaatttt taaaatgaaa aaaatgatga cattcttgaa aaaagctaag gttaaagctt
    18241 ttacattgat tgagatgttg gtggtcttgc tgattatcag cgtgcttttc ttgctctttg
    18301 tacctaatct gaccaagcaa aaagaagcag tcaatgacaa aggaaaagca gctgttgtta
    18361 aggtggtgga aagccaggca gaactttata gcttagaaaa gaatgaagat gctagcctaa
    18421 gaaagttaca agcagatggg cgcatcacgg aagaacaggt taaagcttat aatgaatact
    18481 atactaaaaa tggaggggca aatcgtaaag tcaatgatta aggcctttac catgctggaa
    18541 agtctcttgg ttttgggact tgtgagtatc cttgccttgg gcttatccgg ctctgtccag
    18601 tccacttttg cagcagtaga ggagcagatt ttctttatgg agtttgaaga actctatcgg
    18661 gaaacccaaa aacgcagtgt agccagtcag caaaagacta gtctaaattt agatgggcag
    18721 acgcttagca atggcagtca aaagttgaca gttcctaaag gaattcaggc accatcaggc
    18781 caaagtatta catttgaccg agctgggggc aattcgtccc tggctaaggt tgaatttcag
    18841 accagtaaag gagcgattcg ctatcaatta tatctaggaa atggaaaaat taaacgcatt
    18901 aaggaaacaa aaaattaggg cagtgatttt actggaagca gtagtcgctc tagctatctt
    18961 tgccagcatt gcgaccctcc ttttgggaca aattcaaaaa aataggcaag aggaagcaaa
    19021 aatcttgcaa aaggaagaag tcttgagggt agctaagatg gccttgcaga caggtcaaaa
    19081 tcaggtaaac ataaacggag ttgagattca ggtgttttct agcgaaaagg gattggaggt
    19141 ctaccatggt tcagaacagt tgttggcaat caaagagcca taaggtcaag gcttttacct
    19201 tgttagaatc cctgcttgcc ctgattgtca tcagtggggg cttactcctt tttcaagcta
    19261 tgagtcagct cctcatttca gaagttcgct accagcaaca aagcgagcaa aaggagtggc
    19321 tcttgtttgt ggaccaactt gaggcagaat tagaccgttc gcagttcgaa aaagtagaag
    19381 gcaatcgcct atacatgaag caagatggca aggacatcgc catcggtaag tcaaagtcag
    19441 acgattttcg taaaaccgat gctagtggac gggggtatca gcctatggtt tatggcctca
    19501 aatcagctca gattacagag gacaatcaac tggttcattt tcgtttccag tttcaaaaag
    19561 gcttagaaag ggagttcatc tatcgtgtgg aaaaagaaga aagttaaggc aggtgttctc
    19621 ctctacgcag tcaccatagc agccatcttt agtcttttgt tgcaatttta tttgaaccga
    19681 caaatcgccc actatcaaga ctatgcttta aataaagaaa aattggttgc ttttgccatg
    19741 gctaaacgaa ccaaggataa agccgagcaa gaaagtgggg aacaggtttt taatctaggt
    19801 caggtaagct atcaaaacaa gaaaacaagc ttggtgacga ctgttcgtac gtctaagagc
    19861 caatatgagt ttctgtttcc ttcagtcaaa atcaaagaag agaaaagaga taaaaaggaa
    19921 gaggtagcga ccgattcaag cgaaaaagcg gagaaaaaaa atcagaagag aagcctgaaa
    19981 agaaagagaa ttcctagtca attcaactat aatgcgttga atccagaata gtccactgta
    20041 gtttctagaa aattgctgga aatggatgtt aagctccaat tcatttgttt atatcttatt
    20101 tcagtttact atactttgtg ctaaattaaa gatatgaaac atgattttaa ccacaaagca
    20161 gaaactttcg attcccctaa aaatatcttc ctcgcaaact tggtatgtca agcagccgag
    20221 aaacagattg atcttctatc agacaaagaa attttagatt tcggtggtga cacgggtcta
    20281 ttagccttgc ccctagcccc tagccaagca ggctaagtca gtcactcttg tagacatttc
    20341 tgagaaaatg ttggagtaag ctcgtttgaa agcggagcag caagcaatca agaatatcca
    20401 gtttttggag caagatttac cgaaaaatcc cttggagaaa gagtttgatt gccttgctgt
    20461 tagtcggctt cttcatcata tgcctgattt ggatgcggat ctctcactgt ttcatcaaca
    20521 tttgaaggaa gataggaaac tcatcattgc tgattttacc aagacagaag ctaatcatca
    20581 tggatttgat ttagctgaac tggaaaacaa gctaattgag catggttttt catctgtgca
    20641 tagtcagatt ctctatagcg ctgaagacct gtttcaaggg aatcactcag aattcttttt
    20701 aacagtatcc caaaaatcac tcgcctagtc agggagtgat ttttctataa ggatggaaaa
    20761 aagaagggaa atttgataag ataggaatat ggattttgaa aaaattgaac aagcttatat
    20821 ctatttacta gagaatgtcc aagtcatcca aagtgatttg gcgaccaact tttatgacgc
    20881 cttggtggag caaaatagca tctatctgga tggtgaaact gagctaaacc aggtcaaaga
    20941 caacaatcag gcccttaagc gtttagcact acgcaaagaa gaatggctca agacctacca
    21001 gtttctcttg atgaaggctg ggcaaacaga acccttgcag gccaatcacc agtttacgcc
    21061 agatgctatt gccttacttt tggtatttat tgtggaagag ttgtttacag aggaggaaat
    21121 tactatcctc gaaatgggtt ctgggatggg aattctaggc gctactttct tgacctcgct
    21181 tgataaaaag gtggattact tgggaatgga agtggatgat ttgctgattg atctggcagc
    21241 tagcatggca gatgtaattg gtttgcaggc tggctttgtc caaggagatg ccgttcgccc
    21301 acaaatgctc aaagaaagcg atgtggtcat cagtgacttg cctgtcggct attatcctga
    21361 tgatgccgtt gcgtcgcgtc atcaagttgc ttctagccaa gaacatactt acgcccatca
    21421 cttgctcatg gaacaagggt ttaagtacct taagtcagat ggatatgcta tttttctagc
    21481 tccgagtgat ttgttgacca gtcctcaaag tgatttgttg aaagtctggt tgaaagagga
    21541 ggcaagtctg gttgctatga ttagtttacc tgaaaatctc tttgctaatg ctaaacaatc
    21601 taagactatt tttatcctac agaagaaaag tgaaatagca gtggagcctt ttgtttatcc
    21661 acttgctagc ttgcaagatg caagtgtttt aatgaaattt aaagaaaatt ttcaaaaatg
    21721 gactcaaggt actgaaatat aaaatagatt ttgttataat agttgaaaac gcttaaaaag
    21781 gggtatcatg ttatgacaaa aacaattgca atcaatgcag gaagttcaag tttgaaatgg
    21841 caattatact taatgccaga ggaaaaagtg ttagccaaag gtttgattga acgtatcggt
    21901 ttgaaagatt caatttcaac tgtaaaattt gacggccgtt ctgaacaaca aattttggat
    21961 attgaaaatc atacacaagc cgttaaaatt ttattggatg acttgattcg tttcgatatt
    22021 atcaaggctt atgacgagat tacaggtgtt ggacatcgtg ttgttgctgg tggagaatat
    22081 ttcaaagaat caacagttgt tgagggagat gttttagaaa aagttgaaga gttgagtttg
    22141 ttggctcctc tacacaaccc ggccaatgca gcaggtgttc gtgccttcaa ggaattgttg
    22201 ccagacatta ccagtgtagt tgtttttgat acttccttcc acacaagtat gccagagaaa
    22261 gcttatcgct accctctacc aacaaaatat tacacagaaa acaaggttcg taaatacggt
    22321 gctcatggta caagtcacca gtttgtagca ggagaagctg caaaactctt gggacgtcca
    22381 ttagaagact tgaagttaat tacctgtcat attggtaacg gaggctcaat tacagctgtg
    22441 aaagccggca aatctgtaga cacttctatg gggttcactc ctcttggtgg tattatgatg
    22501 ggaacgcgta caggggatat tgatccagct atcattcctt atttaatgca atatacagag
    22561 gattttaaca caccagaaga tatcagtcgt gttcttaacc gtgaatcagg tcttttggga
    22621 gtttctgcta attctagcga tatgcgcgat atagaagcag ctgtagcaga agggaatcac
    22681 gaggctagct tggcttatga aatgtatgtt gaccgtatcc aaaaacatat cggtcagtac
    22741 cttgcagtgc taaatggagc agatgtcatt gttttcacag caggtgtcgg tgaaaatgca
    22801 gagagtttcc gtcgtgatgt aatctcaggg atttcgtggt ttggttgtga tgttgatgat
    22861 gaaaagaatg tctttggcgt tacaggagac atctcaacag aggcagctaa aatccgtgtc
    22921 ttggttattc caacagatga agaattagtc attgcccgtg acgttgaacg cttgaaaaaa
    22981 taagtgaaac taaaaaaata ttcaatacaa ggagttggga aagttatttt tccagcttct
    23041 ttttctgatg aaattgtcca aaaccttgct atgattggct tttttgaaaa atatggtata
    23101 atagtagtaa tttaatagat ggagttgagt tttgaagaaa aactttcgtg taaaaagaga
    23161 gaaagatttt aaggcgattt tcaaggaggg gacaagtttt gctaatcgca aatttgtggt
    23221 ctaccaatta gaaaaccaga aaaaccattt tcgagtaggt ctatcagtta gcaaaaaact
    23281 ggggaatgcc gtcactagaa atcaaattaa gcgacggatt cggcatatta tccagaatgc
    23341 aaaagggagt ctggtagaag atgtcgactt tgttgtcatt gctcgaaaag gagtcgaaac
    23401 cttgggatac gcagagatgg agaaaaatct actccatgta ttaaaattat caaagattta
    23461 ccgggaagga aatgggagtg aaaaagaaac taaagttgac tagtttgcta ggactgtctc
    23521 tgttaatcat gacagcctgt gcgactaatg gggtaactag cgatattaca gccgaatcgg
    23581 ctgatttttg gagtaaattg gtttacttct ttgcggaaat cattcgcttt ttatcgtttg
    23641 atattagtat cggagtgggg attattctct ttacggtctt gattcgtaca gtcctcttgc
    23701 cagtctttca ggtgcaaatg gtggcttcta ggaaaatgca ggaagctcag ccacgcatta
    23761 aggcgcttcg agaacaatat ccaggtcgag atatggaaag cagaaccaaa ctagagcagg
    23821 aaatgcgtaa agtatttaaa gaaatgggtg tcagacagtc agactctctt tggccgattt
    23881 tgattcagat gccggttatt ttggccctgt tccaagccct atcaagagtt gactttttaa
    23941 agacaggtca tttcttatgg attaaccttg gtagtgtgga tacaaccctt gttcttccga
    24001 ttttagcagc agtattcacc tttttaagta cttggttgtc caacaaagct ttgtctgagc
    24061 gaaatggcgc tacgactgcg atgatgtatg ggattccagt cttgattttt atctttgcag
    24121 tttatgcgcc aggtggagtc gccctatact ggacagtgtc taatgcttat caagtcttgc
    24181 aaacctattt cttgaataat ccattcaaga ttatcgcaga gcgcgaggcc gtagtacagg
    24241 cacaaaaaga tttggaaaat agaaaaagaa aagccaagaa aaaggctcag aaaacgaaat
    24301 aaataaggag gaatctggta atggtagtat ttacaggttc aactgttgaa gaagcaatcc
    24361 agaaaggatt gaaagaatta gatattccaa gaatgaaggc tcatatcaaa gtcatttcta
    24421 gggagaaaaa aggctttctt ggtctatttg gtaaaaaacc agcccaagtg gatattgaag
    24481 cgattagtga aacgactgtt gtcaaagcaa atcaacaggt agtaaaaggc gttccgaaaa
    24541 aaatcaatga tttgaacgag cctgtgaaga cggttagtga agaaaccgtt gaccttggtc
    24601 atgtagttga tgctattaaa aaaatagagg aagaaggtca aggtatttct gatgaagtca
    24661 aggctgaaat cttaaaacat gaaagacatg ccagcactat cttagaagaa actggtcaca
    24721 ttgagatttt aaatgaactt caaatcgagg aagcgatgag ggaagaagca ggcgctgatg
    24781 accttgaaac tgagcaagac caagctgaaa gtcaagaact agaagacttg ggcttgaaag
    24841 ttgaaacgaa ctttgatatt gaacaagtag ctacggaagt aatggcttat gttcaaacga
    24901 ttattgatga catggatgtt gaggctacac tttcaaatga ttataaccgt cgtagcatca
    24961 atttgcaaat tgatactaac gaaccaggtc gtattatcgg ctaccatggt aaagtcttga
    25021 aggccttgca actgttggct caaaattatc tttacaactg ctattcaaga accttctata
    25081 tcactattaa tgtcaatgat tatgttgaac accgtgcaga agtcttgcag acctatgcgc
    25141 aaaaattggc gattcgtgtt ttagaagaag gacgtagtca tcaaacagat ccaatgtcga
    25201 atagtgaacg caagattatc caccgtatta tttcacgtat ggatggcgtg accagttact
    25261 ctgaaggtga tgagccaaat cgctatgtcg ttgtagatac agaataagta aaatcagggt
    25321 tgacctgatt ttttgctagc tagaggaggt taaactgatg ttgaataaga taagagacta
    25381 tttagacttt gctggtttgc agtaccgtaa tcctgataaa gcgggagcag agcgagagaa
    25441 gatgctggca ttccgccaca aaggacaaga ggcccgaaag gcttttacag aactggccaa
    25501 agcctttcaa gcaagccatc cagaatggca actccaacag actagtcagt ggatgaatca
    25561 ggcccagcgt ttgagaccac atttttgggt ttatctacag agagacggac aagtgacaga
    25621 acctatgatg gccttacgtt tgtatgggac atctactgac tttggaattt ctttggaagt
    25681 cagtttcatc gaacgtaaga aggatgagca aacactgggc aagcaggcca aagttttaga
    25741 cattccaacc gttaaaggga tttattatct aacctactct aatggtcaaa gtcaacggtg
    25801 ggaggcgaat gaagaaaagc gtcgtacttt acgcgagaag gtgagaagtc aagaagttcg
    25861 aaaagtttta gtgaaggtag atgttcctat gacagaaaat tcgtctgaag aagaaatcgt
    25921 agaaggctta ttgaagtctt attctaaaat tcttccctat tatctagcta cgagaaaata
    25981 agataatttg taaaacatca taaatcatac agtccaagag tgaacagtcc gctgtgtaat
    26041 tcttggtctt tttgtttgcg ctttcgcatt atataataaa cttacaaaaa caattcaaaa
    26101 ggagaacaat tatggaagtc gtttcaagtg ttctaaattg gttttctagc aatattttgc
    26161 agaatcccgc atttttcgta ggtttattgg tgttgatagg atatgcactt ttgaaaaaac
    26221 ctgcccatga cgttttttca gggtttgtta aagcaacagt agggtatatg ttgcttaacg
    26281 tgggtgctgg tggtttggtt acaacctttc gtccaatctt agcagctctt aactacaaat
    26341 tccaaattgg tgcagcggtt atcgaccctt actttggact tgctgcagca aacaacaaaa
    26401 ttgcagcaga gtttccagat tttgttggaa ctgcaactac agctctattg attggttttg
    26461 gaataaatat cttgctcgta gctcttcgaa agattacgaa ggtaagaacc ctctttatta
    26521 ctggtcacat catggtacaa caagctgcaa cagtatctct tatggttcta ttcttagtac
    26581 cacaattgcg caatgcttac ggtacagcag cgattggtat catctgtgga ctttactggg
    26641 cagttagttc aaatatgact gttgaggcaa ctcaacgctt gactggtggt ggcggatttg
    26701 cgattggtca ccaacagcaa tttgcaatct ggtttgtaga taaagtagca ggacgctttg
    26761 gtaagaaaga agaaagttta gacaatctta aattacctaa gttcctctca atcttccacg
    26821 atacagttgt tgcatctgct accttgatgc tcgtattctt cggagccatt cttttaatct
    26881 tgggtccaga cattatgtct aataaagaag tcatcacttc aggaactcta ttcaatcctg
    26941 ctaaacaaga tttctttatg tacattatcc aaacagcctt taccttctca gtttacttgt
    27001 tcgttttgat gcaaggtgtc cgaatgttcg tatctgagtt gacaaacgcc ttccaaggta
    27061 tttcaaacaa attgttgcca ggttcattcc cagcggttga cgttgcagct tcttatggat
    27121 ttggttctcc aaatgctgtc ttgtcaggat ttacctttgg tttgattggt caattgatta
    27181 caattgtctt gctcatcgtc tttaaaaatc cgattcttat tattacagga tttgtaccag
    27241 tgttctttga caatgcagcc attgcggtct acgctgataa acgcggcgga tggaaagcgg
    27301 ctgttatcct ttcctttata tcaggtgtcc ttcaagttgc tctaggagct ctttgtgtgg
    27361 cccttctcga tttggcatct tatggtggct accatggaaa tatcgacttt gaattcccat
    27421 ggcttggatt tggatatatc ttcaaatacc ttggtattgt tggttatgta cttgtgtgtc
    27481 tcttcttgct tgttattcct caacttcaat ttgccaaagc aaaagataaa gagaaatatt
    27541 acaacggtga agttcaagaa gaagcttagt atctagaaaa ggagaaataa aatggttaaa
    27601 gtattagcag cgtgcggaaa tggaatgggt tcatcaatgg ttatcaagat gaaggttgaa
    27661 aatgctctcc gtaagcttaa tcaaacagat tttacagtca attcatgcag tgtcggtgaa
    27721 gctaaaggtt tagcagtagg atatgacatc gtaatcgctt ctcttcattt gattcaagaa
    27781 ttggaagggc gaactaatgg gaagttaatt gggcttgata acttgatgga tgataaagaa
    27841 atcaccgaaa aactcagtca agcactacag taaaaggttg gagggggctg gacagaaact
    27901 gagagttatc gtttctgtcc ttctccctct ttaaataaag gaggcagata tgaatttaaa
    27961 acaagcttta attgacaatg actcgatccg actaggttta gaggctaaca attggaaaga
    28021 agcagtcaag gtagcagtag atcccttaat tgaaagtggg gcaattttgc cagagtatta
    28081 cgatgctatc attgaatcga ctgaagagta tgggccttac tatatcttga tgccaggtat
    28141 ggctatgccc cacgctagac ctgaagctgg tgtgcaaagt gatgcctttt cattgattac
    28201 cttacaaaat cctgttgtat tttcagatgg gaaagaggta tctgttttgt tggcactagc
    28261 agcaacaagt tcaaaaattc acacaagtgt agccattcca caaattattg ccctgtttga
    28321 attagaagat tctattgcac gtttacaggc ttgccagact aaagaagatg tcttggctat
    28381 gattgaagaa tctaaggata gcccttatct cgaaggattg gatttggaaa gttagaaaga
    28441 ggaataaaga aatgacaaaa agaataccta atttacaagt tgcattagac cattcagact
    28501 tgcaaggagc gattaaagca gctgtttctg ttggtcagga agtagatatt atcgaagctg
    28561 gaactgtttg cttgcttcaa gttggaagtg aactggctga agtcttgcgt agccttttcc
    28621 cagataagat tattgtggca gacacaaaat gtgctgatgc tggtggaaca gttgctaaaa
    28681 ataatgcggt tcgtggagca gactggatga cttgtatctg ttgtgcaacc atccctacta
    28741 tggaagcagc tctaaaggct atcaagactg aacgaggaga acgaggcgaa atccagatcg
    28801 agctttatgg cgattggact tttgaacaag ctcagctttg gctagatgca ggtatctcac
    28861 aagctattta tcaccaatct cgtgatgctc ttcttgctgg tgaaacttgg ggtgaaaaag
    28921 accttaataa ggttaaaaaa ctcattgata tgggcttccg tgtatctgta acaggtggtc
    28981 tagatgtaga tactctcaaa ctctttgaag gtgttgatat ctttaccttt atcgcaggtc
    29041 gtggaattac agaggctgcg gatccagcag gagcagcgcg tgccttcaag gatgaaatca
    29101 aacgaatttg ggggtaaatc atggtacgtc caattggaat ttatgaaaag gcaaccccaa
    29161 cacactttac ttggctagaa cgtttaaatt ttgccaagga gttaggcttt gattttatcg
    29221 agatgtctat tgacgaacgt gacgagcgtt tagcaagact tgactggagt aaggaagaac
    29281 gcttggaagt tgtcaaagaa atctatgaaa ctggtgttcg tattccttct atctgttttt
    29341 caggccatcg tcgctaccca ttgggatcaa aagattcagt tctagaggaa aaatctctag
    29401 aactcatgaa aaaatgtatc gaattagctc aagacttggg agttcgtacg attcaattag
    29461 ctggttacga tgtttactat gaggaaaagt caccccagac acgccaacgt tttatcaaaa
    29521 atttgagaaa agcctgtgac tgggctgaag aagctcaggt ggtacttgct attgaaatta
    29581 tggatgatcc tttcatcagt agcatcgaaa aatatttggc tatagaaaaa gagattgact
    29641 ctcccttcct ctttgtatat ccagatattg gtaatgtgtc tgcatggcat aatgatatct
    29701 atagtgagtt ttatcttggt catcatgcca tcgcagctct ccatctcaag gatacttatg
    29761 cagtgacaga aagttcaaag ggccagttcc gagatgtacc tttcgggcaa ggttgtgtca
    29821 aatgggaaga agctttcgat attttaaagg aaaccaatta taatggacct ttcctaatcg
    29881 aaatgtggtc tgaaaattgt gaaacagtag aagaaacacg cgcagccatt caagaggcgc
    29941 aagcttttct ctatccactc attaagaaag caggtttgat gtaagatgaa tcaagtaatc
    30001 aatgctatgc gtaaacgagt ctgtgatgcc aatcaatcat tgccaaaaca tggacttgtc
    30061 aaatttacct gggggaatgt atctgaagtc aatcgcgaac tcggtgtcat tgttatcaaa
    30121 ccatcaggcg tggattatga cgaattgaca cctgaaaaca tggtagtgac tgatctagat
    30181 ggtaagatcc tagaagggga tttaagacca tcttccgacc tcccaactca tgtgcaatta
    30241 tataagactt ggtcagaaat tggtagtgtg gttcacaccc attcgacaga agctgttgct
    30301 tgggctcagg caggtcgtga tattcctttc tacggaacaa cccatgcaga ttatttctac
    30361 ggttcaattc cttgcgcccg tagtttgacc aaggacgaag tagaagtggc ctatgaaaaa
    30421 gatactggcc tggttatcgt agaagagttt gaacatcgcg gacttaaccc ggttgaagta
    30481 ccaggaattg ttgtacgcaa tcacggtcca ttcacctggg gcaaaaatcc agagaatgct
    30541 gtttatcact ctgtcgtact agaggaagta tcaaagatga atcgctttac agaacaaatc
    30601 aatccaagag ttggacctgc tccccagtac atactagaaa aacactacca acgtaaacat
    30661 ggaccaaatg cttattatgg tcaaaagtaa gaacgatgaa ggaggagaaa aagataaatt
    30721 tagctcctct ttttacattt gatttttatt gagagtaaag ttggagttga agtaatttta
    30781 aaagattttt tagaaatagc gcttgatata tatatggtaa aataaaaaga attgctgtga
    30841 tatcaataga tttgggggat tttttaatat ggtactggat aaggcaagtt gtgatttgct
    30901 tcaatatttg atggatcaag aaacgtccaa aacgattatg gcgatttcga aagatttgaa
    30961 agagtcaaga aggaaaattt attatcacat tgacaaaatc aatgctgctc tgggtgacga
    31021 ggcgcttcac atcattagta ttccacgaat tggtattcac ttaacggaag agcagagaga
    31081 tgcttgttgt aaactattat cggaagtaga ttcgtacgat tatatcatga gtgcgcatga
    31141 acgtatgatg ataatgttac tatggatagg tatttctaaa gaacgtatta cgattgaaaa
    31201 attgatagag ttaacagagg tatctaggaa tactgttctc aatgatttga atagtattcg
    31261 ttatcaacta actttggaac aatatcaggt gatcttgcaa gtgagcaagt cacagggata
    31321 ccaccttcat gcccaccctc ttaataaaat tcagtatctt caatcgcttc tatatcatat
    31381 ttttatggaa gaaaatgcca cttttgtatc tattttagaa gataagatga aagagaggtt
    31441 agatgatgag tgtttgcttt ctgttgaaat gaaccaattt tttaaggaac aggttccttt
    31501 agttgaacaa gatttaggga agaaaataaa tcatcatgaa ataactttta tgttgcaggt
    31561 tctaccttat ttgctgttaa gctgtcataa tgttgaacag tatcaagaaa gacatcagga
    31621 tatagagaaa gaattttctt tgataagaaa aagaatagag tatcaggtgt ctaagaaatt
    31681 aggagaacgg ttgtttcaaa agtttgaaat ttctttgtca ggacttgaag tttctcttgt
    31741 agctgttctc ctcctctcct atcgtaaaga tttggatatt catgcagaaa gtgatgattt
    31801 tcggcaatta aaacttgctt tagaagaatt tatctggtat tttgaatcac aaatccgaat
    31861 ggagattgag aacaaggatg atttgttacg aaatttgatg atccactgta aagccttgtt
    31921 atttagaaag acttacggta ttttttctaa aaatcctcta acaaaacaaa ttcgatccaa
    31981 gtatggagaa ttatttttag tcactagaaa atctgcggaa attttagaag gagcatggtt
    32041 tattcggcta acagacgatg atattgccta tttgacgatt catattggag gatttttaaa
    32101 gtatacacca tcgtctcaaa aaaatatgaa aaaagtttat ctcgtttgtg atgaaggtgt
    32161 tgcggtttcg agacttttgc tgaaacaatg caaactttat tttccaaatg agcaaattga
    32221 cactgtattt acaacagaac aatttaagag tgtggaagat attgcacaag ttgatgtagt
    32281 gattactact aatgatgatt tggatagcag atttccgatt ttaagggtta atcctatcct
    32341 tgaagcagaa gatattttga aaatgctaga ctatcttaaa cacaatatat ttcgtaataa
    32401 gagcaaaagt ttcagtgaaa atctttctag tcttatttcg tcttatattg tagacagcaa
    32461 gttggctagt aagttccaag aagaggttca aacacttata aatcaagaaa tagtagttca
    32521 agcttttttg gaagatattt gaaggacagt ccaatgatga acacaaacct gtgtttttct
    32581 tggtcttttt tagtgttttg aagggtggta tactaatctc aaagataaca attatatcca
    32641 aaggaggcaa catatgccaa acgtcaaaga aattacaaga gagtcatgga ttttagccac
    32701 tttcccagag tggggaacat ggttgaacga agaaatcgaa gaagaagtcg tacctgaagg
    32761 caactttgcc atgtggtggc taggcaactg tggtacttgg attaagacac cagctggtgc
    32821 taacgttgtc atggaccttt ggtcaaaccg tggaaaatca accaaaaaag tgaaagatat
    32881 ggttcgtggg caccaaatgg caaatatggc aggtgttcgt aaattgcaac caaacttgcg
    32941 tgttcagcca atggttatcg atccatttgc tatcaacgaa ctagactatt acttagtttc
    33001 acacttccac agtgatcata tcgacccata cacagctgca gcaattctca acaatcctaa
    33061 gttagagcat gttaagttta tcggtcctta ccactgtgga cgaatctggg aaggatgggg
    33121 ggttccaaaa gaacgtatca tcgttgttaa accaggtgac actatcgaat taaaagatat
    33181 gaagattcat gcagtagaat catttgaccg tacttgcttg gtaactctcc cagtgaacgg
    33241 tgctgatgag acaggcggtg aacttgctgg cttggctgtt acagatgaag aaatggctca
    33301 aaaggctgtt aactatatct ttgaaacacc aggtggaacc atctatcatg gtgcagattc
    33361 tcacttctca aactattttg caaaacatgg taaagacttt aaaattgatg ttgctttgaa
    33421 taactatggt gaaaatccgg taggtatcca agacaaaatg acatctatcg accttcttcg
    33481 tatggcagaa aatctgcgta ccaaagtcat tatcccagtt cactatgata tctggtctaa
    33541 cttcatggct tctactaatg agattctaga actttggaaa atgcgaaaag atcgcttgca
    33601 atacgatttc catccattta tctgggaagt tggcggtaag tacacttatc ctcaagatca
    33661 acacttagta gaataccatc atccacgtgg ttttgatgat tgttttgaac aagactctaa
    33721 cattcaattt aaagctttgc tataatataa aaatattctt tggaggttat ccgtatgttg
    33781 catgatacgg ataattttca tataatagta aaatagaatg tgtgattcaa taatcacctc
    33841 aaatagaaag gaaattctat gtcaaatcta tctgttaatg caattcgttt tctaggtatt
    33901 gacgccatta ataaagccaa ctcaggtcat ccaggtgtgg ttatgggagc ggctccgatg
    33961 gcttacagcc tctttacaaa acaacttcat atcaatccag ctcaaccaaa ctggattaac
    34021 cgcgaccgct ttattctttc agcaggtcat ggttcaatgc tcctttatgc tcttcttcac
    34081 ctttctggtt ttgaagatgt cagcatggat gagattaaga gtttccgtca atggggttca
    34141 aaaacaccag gtcacccaga atttggtcat acggcaggga ttgatgctac gacaggtcct
    34201 ctagggcaag ggatttcaac tgctactggt tttgcccaag cagaacgttt cttggcagcc
    34261 aaatataacc gtgaaggcta caatatcttt gaccactata cttacgttat ctgtggagac
    34321 ggagacttga tggaaggtgt ctcaagcgag gcagcttcat acgcaggttt gcaaaaactt
    34381 gataagttgg ttgttcttta tgattcaaat gatatcaact tggatggtga gacaaaggat
    34441 tcctttacag aaagtgttcg tgaccgttac aatgcctacg gttggcatac tgccttggtt
    34501 gaaaatggaa cagacttgga agccatccat gctgctatcg aaacagcaaa agcttcaggc
    34561 aagccatctt tgattgaagt gaagacggtt attggatacg gttctccaaa caaacaagga
    34621 actaatgctg tacacggcgc ccctcttgga gcagatgaaa ctgcatcaac tcgtcaagcc
    34681 ctcggttggg actacgaacc atttgaaatt ccagaacaag tatatgctga tttcaaagaa
    34741 catgttgcag accgtggcgc atcagcttat caagcttgga ctaaattagt tgcagattat
    34801 aaagaagctc atccagaact ggctgcagaa gtagaagcca tcatcgacgg acgtgatcca
    34861 gtcgaagtga ctccagcaga cttcccagct ttagaaaatg gtttttctca agcaactcgt
    34921 aattcaagtc aagatgcctt gaatgttgtg gcagctaaat taccaacctt cctaggtgga
    34981 tcagctgacc ttgctcactc aaacatgact tatatcaaga cggatggact tcaagacgat
    35041 accaatcgct tgaatcgtaa cattcagttt ggtgttcgtg aatttgcaat gggaacaatc
    35101 ttgaacggga tggcccttca tggtggactt cgtgtatacg gtggaacttt cttcgtcttc
    35161 tctgactatg tgaaggcagc tgtccgcttg tcagccttac aaggacttcc tgtgacttat
    35221 gtctttaccc atgattcaat cgcagttggg gaagatggtc cgactcacga accggttgag
    35281 catttagcag gtcttcgtgc tatgccaaat ctaaatgttt tccgtccagc agatgcgcgt
    35341 gaaacgcaag cagcttggta ccttgcagtg acaagtgaga aaacaccaac tgcccttgtc
    35401 ttgacacgtc aaaacttgac tgttgaagat ggaacagact tcgacaaggt tgctaaaggt
    35461 gcttatgttg tatatgaaaa tgcagccgac tttgatacca tcttgattgc gacaggttca
    35521 gaggttaatc ttgctgtctc agctgctaaa gaattggcta gtcaaggcga aaaaatccgc
    35581 gtagtcagca tgccatctac agatgtcttt gataaacaag atgcagctta caaggaagag
    35641 attcttccaa atgcagtccg ccgtcgtgtt gcagtcgaaa tgggtgcaag tcaaaactgg
    35701 tacaaatatg ttggtctcga tggtgccgtt ctaggtatta ataccttcgg agcctctgcc
    35761 ccagcaccaa aagtattggc agaatatggc tttactgtag aaaatcttgt aaaaattgtt
    35821 cgaaacttga aataatccta aaaatcaggg cgtaagctct ggtttttctt accagaaaag
    35881 taaggtacaa tcttgtaaaa gtagctgaaa tttgatatag tagtcctatg taaaagacaa
    35941 aggagaaaac aatgaatcca aatattactt ttttaatcat gcttgtaggt atgatggcct
    36001 tgatgttctt tatgcaacgt tctcaaaaga aacaagctca aaagcgtatg gaaagcttaa
    36061 ataaactaca aaaaggctat gaagtgatta caatcggtgg actttatgga acagtcgatg
    36121 aagtagatac ggagaaggga acaatagttc ttgacgtaga tggagtttac ttgacttttg
    36181 aactagctgc tatcaagaca gtattaccgc tgaaagaaac agcttcacta gaaggcgcaa
    36241 ttgaaaaata agacgggatc acgaactccc gtttttctat aaaagaaagg aaatgggatg
    36301 aaaaaattag tctttgtctg tctgggaaat atttgccgta gccctatggc cgagtttgtt
    36361 atgaaatcaa tgacagataa ctacgaaatc caaagtcgag caacttcctc ttgggaacat
    36421 ggcaatccga ttcataaggg gactcaggga atttttcaag agtatgagat tccttatgac
    36481 aagaacaaga catcgcttca gattagtaag gaagattttg aagcctttga ttatattatc
    36541 ggaatggacg cttcaaatat ttctgactta cgtcagatgt gtccagtaga ctgtcaagat
    36601 aagatttact cattttcatc tgaaagtgtt ccagaccctt ggtatacagg agattttgaa
    36661 gaaacctatc gacgtgttca agagggctgt caagcttggt tagaacgttt agaaaaggag
    36721 agttaaggtg gaaaatctta cgaattttta cgaaaagtat cgtgtctatc tgactcgtcc
    36781 acgattagag cttttggcag tagttaccat tgttttctgt gctgtactcg tcttttttct
    36841 aaatattcca ggaaaaggtg tcttaaaact cgataatgga acgattgttt atgatggcag
    36901 tcttgtccgt ggtaaaatga atggtcaagg taccattacc ttccaaaatg gagaccaata
    36961 tacaggtggc ttcaacaatg gagccttcaa cggaaaaggt acctttcaat ctaaagaagg
    37021 ctggacctac gaaggtgatt ttgtaaatgg tcaggctgaa ggaaaaggga aactaacaac
    37081 agaacaagaa gtcgtttatg aaggaacttt taaacaaggc gtttttcaac aaaaataaag
    37141 cctccttatc aaaggaggta ttattagaat cgcaaggtaa gcgcttacct gcaaatccct
    37201 ttctttccaa atccctcttc caaccaagtt tgtgaaataa aaaatatttg aaataaattt
    37261 cacaaacttc aaagataaaa cctgataaga aaagaagatg agaaaagttt cacaagagtt
    37321 taaaaattct ttgtgaaatg tttatgaaac tgtttacaaa gttgaaataa tgcgttataa
    37381 taaacttgtg aaaaaattaa caaaggatat cgttccttga aagctatgga ggaaaatatg
    37441 gctgataaaa aaactgtgac accagaggaa aagaaactcg ttgctgaaaa acacgtagat
    37501 gagttggttc aaaaagctct agttgccctt gaagaaatgc gtaaattgga tcaagaacaa
    37561 gttgactaca tcgttgccaa agcatcagta gcagctttgg atgcccacgg agaattggct
    37621 ttacatgcct ttgaagaaac aggacgtggt gtatttgaag ataaagcaac taagaacttg
    37681 tttgcttgtg aacacgtggt aaacaacatg cgtcacacta aaacagttgg cgttatcgaa
    37741 gaagatgatg taacaggatt gaccctgatt gctgaaccag ttggtgttgt ttgtggtatc
    37801 actccaacaa caaacccaac atcaacagcg atcttcaaat cattgatttc attgaagaca
    37861 cgtaatccaa tcgtctttgc cttccatcca tcagctcaag aatcatcagt tcatgcagct
    37921 cgtatcgtcc gcgatgcagc tatcgcagct ggtgctcctg aaaactgtgt acagtggatc
    37981 actgaaccat ctatggaagc aactagtgcc cttatgaacc acgaaggtat tgcaacaatc
    38041 cttgcaacag gtggtaatgc catggttaaa gccgcatact catgtggtaa accagctctt
    38101 ggggtaggtg ccggaaacgt tccagcttat gttgaaaaat cagcaaacat tcgtcaagca
    38161 gcacacgata tcgtcatgtc taaatcattt gataacggta tggtctgtgc atctgaacaa
    38221 gcagttatca ttgataaaga aatttacgat gaatttgtag cagagttcaa atcttaccac
    38281 acttactttg taaacaaaaa agaaaaagct cttcttgaag agttctgctt cggcgtcaaa
    38341 gcaaacagca aaaactgtgc tggtgcaaaa ttgaacgctg acatcgttgg taaaccagca
    38401 acttggattg cagaacaagc aggatttaca gttccagaag gaacaaacat tcttgctgca
    38461 gaatgtaaag aagttggcga aaatgagcca ttgactcgtg aaaaattgtc accagttatt
    38521 gcagttttga aatctgaaag ccgtgaagat ggtattacta aggctcgtca aatggttgaa
    38581 tttaacggtc ttggacactc agcagctatc cacacagctg acgaagaatt gactaaagaa
    38641 tttggtaaag ctgttaaagc tattcgtgtt atctgtaact caccttctac ttttggtggt
    38701 atcggggacg tttacaatgc cttcttgcca tcattgacac ttggatgtgg ttcttacgga
    38761 cgcaactcag ttggggataa cgttagtgcc attaacctct tgaatatcaa aaaagtcgga
    38821 agacggagaa ataacatgca atggatgaaa cttccttcaa aaacatactt tgaacgtgat
    38881 tcaattcaat accttcaaaa atgtcgtgac gttgaacgtg tcatgatcgt tactgaccat
    38941 gccatggtag agcttggttt ccttgatcgt atcatcgaac aactggacct tcgtcgcaat
    39001 aaggttgttt accaaatctt tgcggatgta gaaccggatc cagatatcac aactgtaaac
    39061 cgtggtactg agattatgcg tgccttcaga ccagatacca tcatcgcact cggtggtggg
    39121 tctccaatgg atgctgccaa agtaatgtgg ctcttctacg agcaaccaga agtggacttc
    39181 cgtgaccttg tccaaaaatt catggatatc cgtaaacgtg ccttcaagtt cccattgctt
    39241 ggtaagaaga ctaaattcat cgcgattcca actacatctg gtacaggatc tgaagtaaca
    39301 ccatttgccg ttatctctga taaagcaaac aaccgtaaat acccaatcgc tgactactca
    39361 ttgacaccaa ctgtggcaat tgtagaccct gctttggtat tgacagttcc aggatttgtt
    39421 gctgctgata ctggtatgga cgtattgact cacgcgacag aagcatacgt atcacaaatg
    39481 gctagtgact acactgatgg tttagcactt caagctatta aattggtctt tgaaaatctc
    39541 gaaagctcag ttaagaatgc agacttccac tcacgtgaga aaatgcataa cgcttcaaca
    39601 atcgctggta tggcctttgc caatgccttc ctaggtattt ctcactcaat ggcccataag
    39661 attggtgcgc aattccacac aatacacggt cgtacaaatg ctatcttgct tccatacgtt
    39721 atccgttaca acggtacacg tccagctaag acagcaacat ggcctaagta caactactac
    39781 cgtgcagatg aaaaatacca agatatcgca cgcatgcttg gacttccagc ttctactcca
    39841 gaagaagggg ttgaatctta cgcaaaagct gtctacgaac tcggtgaacg tattgggatc
    39901 caaatgaatt ttagagacca aggaattgac gaaaaagaat ggaaagaaca ttctcgtgaa
    39961 ttagccttcc tggcttatga agaccaatgt tcaccagcta acccacgtct tccaatggta
    40021 gaccatatgc aagaaatcat cgaagatgca tactatggct acaaagaaag accaggacgc
    40081 cgtaaataat tgtttatcag tctagaagca agacaaaaac tcaatttgag ggaaagatcc
    40141 agtaattttt ctatgataaa aggcatccta tcaaggtttt tgaacacctg ataggatgcc
    40201 tttttatgat attgaggcct ttttgccctt tttgaaaaac tagaatagaa acaaaatata
    40261 taatagattg aaactggaat agtacacttc tgcttctaaa acattgttag aattcgattt
    40321 gactgtcctg atcgatttgt cctatt
//
LOCUS       DAVLRT010000011        39088 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLRT010000011 DAVLRT010000000
VERSION     DAVLRT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.011"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            549..2470
                     /locus_tag="VXM85_000537"
                     /pseudo
     CDS             549..2470
                     /locus_tag="VXM85_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            2451..2654
                     /locus_tag="VXM85_000538"
     CDS             2451..2654
                     /locus_tag="VXM85_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345165.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMITSNFLSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            2657..3007
                     /locus_tag="VXM85_000539"
     CDS             2657..3007
                     /locus_tag="VXM85_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345166.1"
                     /translation="MAETNQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKSKE"
     gene            3017..3316
                     /locus_tag="VXM85_000540"
     CDS             3017..3316
                     /locus_tag="VXM85_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345167.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHAVGDIQKEMVRMNDRMEEIEKGE"
     gene            3320..3649
                     /locus_tag="VXM85_000541"
     CDS             3320..3649
                     /locus_tag="VXM85_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1345168.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKG"
     gene            3655..4611
                     /gene="lytA"
                     /locus_tag="VXM85_000542"
     CDS             3655..4611
                     /gene="lytA"
                     /locus_tag="VXM85_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1345169.1"
                     /translation="MDIDTSRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLAD
                     RWKKYTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPDGLITVK"
     gene            4843..5085
                     /locus_tag="VXM85_000543"
     CDS             4843..5085
                     /locus_tag="VXM85_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345170.1"
                     /translation="MKILNIKLANVEQTDSGFEHWVDVTYNVPILKNEYTVRLLLLFG
                     FEIEDPEVIEYLVNAWKYRELVLHSVRMYDMEREAS"
     gene            complement(5152..6579)
                     /locus_tag="VXM85_000544"
     CDS             complement(5152..6579)
                     /locus_tag="VXM85_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1345171.1"
                     /translation="MKTTNKVAIYVRVSTTSQVEEGYSIDEQKDKLEAYCKIKDWKIY
                     DVYVDGGFSGANTQRPELERLISDVKRKKVDIVLVYKLDRLSRSQKDTLFLIEDVFAK
                     NDVAFISLQENFDTSTPFGKASIGMLSVFAQLEREQIKERMMLGKEGRAKNGKSMSWT
                     TIPFGYDYSKETGVLSVNPTQALIVNRIFTEYLNGKPVVKIIRDLNAEGHVGRKRPWG
                     ETITKYLLKNETYLGKVKYKDKVYEGQHEPIITQELFDLVQLEVERRQISAYEKYNNP
                     RPFRAKYMLSGLMKCGYCGASLGLRYTRKDKNGISHHKYQCRNRHSKDLEKRCESGWY
                     SKEELERIVIKELERIKFDPKYKNETLAKKEETIKVEEIKKQLERINNQVSKLTELYL
                     DEIITRKELDEKNDKIKTERQFLEEQLENQKSNVLSIRKRKLTRLLKDFDVEKLSYED
                     ASKIVKNIIKEIIVTKDGMSITLDF"
     gene            complement(6747..6947)
                     /locus_tag="VXM85_000545"
     CDS             complement(6747..6947)
                     /locus_tag="VXM85_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345172.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            complement(6949..7740)
                     /locus_tag="VXM85_000546"
     CDS             complement(6949..7740)
                     /locus_tag="VXM85_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEV1345173.1"
                     /translation="MARGRGKLTPQDKEDMKVFSANLNSILSDRNCKQAELSRATGIP
                     PSTLTGYVKGTSLPIPGNVQKIADFFGVPKSVLDPRFITNNFMVNDSSSNTPQIQTIY
                     DELEPPRQGKVLNYAKRQLKEQKNEEETKINEVSEAIRLYSYDYYDHPASAGTGQYLN
                     DVRVERIELPVDVDADFVIPIKGDSMEPDYHDGDLVFIQTSVDLNDGVIGVFNYNGDA
                     YIKQLVIDEDQAYLHSLNPEYKDMPITPDTDFRIIGEVVDLYREK"
     gene            7913..8188
                     /locus_tag="VXM85_000547"
     CDS             7913..8188
                     /locus_tag="VXM85_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345174.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEENFLYSQLEAFCTNCRQF
                     AFGWTELAEEIERQERYLLDSGFTQEEIDDIRFDAAFAGMLDKLNVA"
     gene            8263..8430
                     /locus_tag="VXM85_000548"
     CDS             8263..8430
                     /locus_tag="VXM85_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345175.1"
                     /translation="MNESFLTILGISMIASFITNLIAYLAGKHHLKKKIKNHKMWFDS
                     EIERIKKKYHL"
     gene            complement(8421..8870)
                     /locus_tag="VXM85_000549"
     CDS             complement(8421..8870)
                     /locus_tag="VXM85_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345176.1"
                     /translation="MNNFSFIETLAIAAIPAFVSGMWSYIAAKGNSKHEIDKINIAHS
                     QELENVENQFKQDMEKMQKQHSQELYSLQQTHELRLLELEKVSQLDTQTDQGLKINDL
                     IFKAISGEISADVAIKNMSTLSHFANKQQPSDLQKQFVKKLSKKNHK"
     gene            9069..9239
                     /locus_tag="VXM85_000550"
     CDS             9069..9239
                     /locus_tag="VXM85_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345177.1"
                     /translation="MKEKLNEFLKFRSQFTKREWFEINQTVEARLNQKADHLKLDDVD
                     LEIISKRLGRSI"
     gene            complement(9464..9670)
                     /locus_tag="VXM85_000551"
     CDS             complement(9464..9670)
                     /locus_tag="VXM85_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345178.1"
                     /translation="MTDYQLEASLIVLGKEFDRTKKNGKESFSVHVSFFDGLDANQHL
                     QEFARQYPVKIDRSNSDQITFLIK"
     gene            9743..9970
                     /locus_tag="VXM85_000552"
     CDS             9743..9970
                     /locus_tag="VXM85_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1345179.1"
                     /translation="MPKMTLRAIRTNYNLSAKEVADKLNIHQQTLLKYEHDSSKIPMD
                     LLDKLARLYNVEKDFIFLGKKYELNHNLGEV"
     gene            10013..10375
                     /locus_tag="VXM85_000553"
     CDS             10013..10375
                     /locus_tag="VXM85_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1345180.1"
                     /translation="MQYIIRNTHGNYTSLNNAYAQDKRLKATTIGILTVILMNKSDWV
                     VYPDEIARRLGISRRTVDEHFKLLEKAGYLRVYRLGLGRGKGVTVYRFFSDMPISDDY
                     FEYLKTNLEKELSTDNGV"
     gene            complement(10785..10976)
                     /locus_tag="VXM85_000554"
     CDS             complement(10785..10976)
                     /locus_tag="VXM85_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345181.1"
                     /translation="MKSFKDFRESLTAEDMQAISAKANEATKQIDHTDGLQLGKVSGL
                     TSVITTIELLEKYHEWLHS"
     gene            11095..11238
                     /locus_tag="VXM85_000555"
     CDS             11095..11238
                     /locus_tag="VXM85_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345182.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     KTV"
     gene            11305..12102
                     /locus_tag="VXM85_000556"
     CDS             11305..12102
                     /locus_tag="VXM85_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEV1345183.1"
                     /translation="MNMNLNTNEILTTTEYDMFRKISNRKITENPKLEEELLSEGQRQ
                     PILVNEKMEVIDGQHRLYYLRKHRKPVRYIIDPTANFKTVISMNTSAVNWALQDYVYS
                     FALEGDPEFVKLAKFLDENELLSDKMVIVAGSGRRDGTAAHVVKKLKKGDYVFSNEKQ
                     LREFCKFYERVLNETKLPNKPFLQSVLWTLYTTSVFDENRMLTQLKKSDLTSEDIEGF
                     AKKKLLLTFLELYNGRWSDDHPSLIQYFINRKGSLTIPSLPKQDEDN"
     gene            12120..12257
                     /locus_tag="VXM85_000557"
     CDS             12120..12257
                     /locus_tag="VXM85_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345184.1"
                     /translation="MKVTVYVYGRKLEPDEPIIIPENHRFYDIWNGIVNEMLDNEEDV
                     A"
     gene            12257..12478
                     /locus_tag="VXM85_000558"
     CDS             12257..12478
                     /locus_tag="VXM85_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345185.1"
                     /translation="MKLLTKLKLRLEGVLKSVNLDWREVAVELMNDLFEERKRRFAFE
                     QENYDLKQELAAYKYKENFDIKARLQGEM"
     gene            12479..12733
                     /locus_tag="VXM85_000559"
     CDS             12479..12733
                     /locus_tag="VXM85_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345186.1"
                     /translation="MYIISIYVKNTETGNEDFSLIGRDFLPTGHQDYIARVFGTKEEA
                     IDYLKSISYIASGVHGNDWVYQNEKLPEIESRCRIWKVGE"
     gene            12746..13006
                     /locus_tag="VXM85_000560"
     CDS             12746..13006
                     /locus_tag="VXM85_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345187.1"
                     /translation="MFKALKTIKKIKQLQKEMHAFSLAFLALQDMGLMPETERSKAKA
                     QTMHDVSRVLKDVLDGKSVDEAIKRLNSEVKAEEVGQKDDQN"
     gene            13276..14094
                     /gene="bet"
                     /locus_tag="VXM85_000561"
     CDS             13276..14094
                     /gene="bet"
                     /locus_tag="VXM85_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEV1345188.1"
                     /translation="MTNNQIVEAKGDFLTNPQLLNSGIIRKYLDPQGKASDEELAYFI
                     AQAKAQNLNPFTKEIYFIKYGTQPAQIVTAKSAFEKKADSHPQFDGKEAGVIYLMDGE
                     IKYSKGAFIPKGAEILGGWAKVYRKDRTYPTETEVSFEEYDNSKIRARVKELTQQGKD
                     VTYPVMNSYGKPIGENNWDTMPCVMIRKVALVSAYREAFPAELGASYEADEIQLDNTP
                     KDVTPQESREDVVARKMAQIEQFNKEQEANHADPEPAKTEEPIQGELLDGELEY"
     gene            14104..15192
                     /locus_tag="VXM85_000562"
     CDS             14104..15192
                     /locus_tag="VXM85_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEV1345189.1"
                     /translation="MQELQVIDDKKINKIYEMITTDELTRESFEKDLIEATEKYKDYI
                     PTAGTLKDDKAKRAEFNKLIESKNRIRIDTKNLLSETANTWDSYAKSIIDPFATVVSE
                     FDKGIKEIEEHQRQLKIDTVKSYLANKSAEYMLDPRLFDEKALEYVKAGDFMADGVTL
                     KKATMKSLDDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTLAEAFEQ
                     IKADYEFEKQKEEIRQAQERAERESQELLAAQQTKQQERAPKSTGTPNFDPEAGEILD
                     GEQIPQNEQNSLRGAENDLKRYTQKMTLEVYFVDTAEKDRFKAGLSQLGFDFKKNYQV
                     SGYQRIEPLTQAELNEQWGGNHGHQKNI"
     gene            15170..15493
                     /locus_tag="VXM85_000563"
     CDS             15170..15493
                     /locus_tag="VXM85_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345190.1"
                     /translation="MDIRKISDSVAIYSDGKKLHVIHNLGDEFILDFKVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEIFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            15486..15821
                     /locus_tag="VXM85_000564"
     CDS             15486..15821
                     /locus_tag="VXM85_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEV1345191.1"
                     /translation="MIKTVFFSCDYPHHEAIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            15916..16332
                     /gene="ssb"
                     /locus_tag="VXM85_000565"
     CDS             15916..16332
                     /gene="ssb"
                     /locus_tag="VXM85_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1345192.1"
                     /translation="MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFKAWRGTADIIAQYCSKGSLIGIIGRLQVRSYEKDGQRRYVTEVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            16345..17040
                     /locus_tag="VXM85_000566"
     CDS             16345..17040
                     /locus_tag="VXM85_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV1345193.1"
                     /translation="MEIDKIIKKDVLEFMETIPDNKIDLIVTDPPYLINYKTNWRKEK
                     HKFSNVIKNDNNPELIKEYIKECYRILKDDTAIYIFCSFDKVDFFKKEIEKYFSVKNI
                     IIWRKNNHTAGDLEAQFGKQYEMIILANKGRKKFNGERLTDVWDFKRVSSDKLLHQNQ
                     KPIELIKRCIVKHSDVGDTVFDGFMGSGTTALAALETDRHFIGTEIDEYYFGIAEERI
                     KNHNAQLSLFDEV"
     gene            17042..17251
                     /locus_tag="VXM85_000567"
     CDS             17042..17251
                     /locus_tag="VXM85_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345194.1"
                     /translation="MEWTDWVDWKPETKTDIKTKIENDGYTFPHYDKKNNGVKYVIST
                     LDIKRDCLRLGVPFEDVYPLQTTLF"
     gene            17265..17594
                     /locus_tag="VXM85_000568"
     CDS             17265..17594
                     /locus_tag="VXM85_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345195.1"
                     /translation="MASKINATERIAIIIDKQKIEVVTTLNYDMSISFDNKDTAPTLD
                     DNGDLFEPVYKCKVKAIPKNDVFFTSLTRVKSNIKTLQEVKKFFEFVNENRENLFEMA
                     GFKGALE"
     gene            17591..18022
                     /locus_tag="VXM85_000569"
     CDS             17591..18022
                     /locus_tag="VXM85_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEV1345196.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRARLRFYIKPPQYISKVKKNQQALLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            18038..18220
                     /locus_tag="VXM85_000570"
     CDS             18038..18220
                     /locus_tag="VXM85_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345197.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            18245..18562
                     /locus_tag="VXM85_000571"
     CDS             18245..18562
                     /locus_tag="VXM85_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345198.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWIGYSPSMAQVVIAVYLIY
                     GGN"
     gene            18564..19052
                     /locus_tag="VXM85_000572"
     CDS             18564..19052
                     /locus_tag="VXM85_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1345199.1"
                     /translation="MNKQELIEKYKKLEGVWNAEGAELARQIFLQDLEQLDKPKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPEWFYENNIEKFCLAWLDGYEVEKE
                     KRYLVTLKNRQPLVKSQSGSTLYFSQDITARNYKGTQKELEDANFGWVFDCPGIEIEE
                     VE"
     gene            19210..19470
                     /locus_tag="VXM85_000573"
     CDS             19210..19470
                     /locus_tag="VXM85_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEV1345200.1"
                     /translation="MKRFIAIWILLSAGLNIWQSIYIKKLEEKRPIVVYKADNAGAEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYDKVKVGDEVLL"
     gene            19467..19889
                     /locus_tag="VXM85_000574"
     CDS             19467..19889
                     /locus_tag="VXM85_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345201.1"
                     /translation="MTETIKLPDYYEPDWKNARYGSLEELKELLLFKRIVKWDKDFLL
                     LEDGTKVTIEMSESDCCASAGGEFQDVSLDAVITNIEIGEPEEIPDHWGTGYKNKVTI
                     FHNQNPVAIANCEAEHNGYYYSVCSLVIGDIHFPVVNA"
     gene            19901..20338
                     /locus_tag="VXM85_000575"
     CDS             19901..20338
                     /locus_tag="VXM85_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345202.1"
                     /translation="MTPRYRAWIKTEKRMFFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYDFDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYDKEFIG
                     IVKQLEGSWVIDTGSDAVCLWTEIEENEIIGNIYENKEFGGRK"
     gene            20335..20706
                     /locus_tag="VXM85_000576"
     CDS             20335..20706
                     /locus_tag="VXM85_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345203.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFKH"
     gene            20779..21183
                     /locus_tag="VXM85_000577"
     CDS             20779..21183
                     /locus_tag="VXM85_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1345204.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSEVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            21264..21346
                     /locus_tag="VXM85_000578"
     tRNA            21264..21346
                     /locus_tag="VXM85_000578"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21295..21297,aa:Tyr,seq:gta)
     gene            21651..22091
                     /locus_tag="VXM85_000579"
     CDS             21651..22091
                     /locus_tag="VXM85_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV1345205.1"
                     /translation="MSGLRIKQKRFADEYIISGNATEAYKKAGYRVSSDRVAGVEGHK
                     LLKNPKIKSYIDERLKQLDSEKIADQQEVLGYLTSVMRGETQEQTLISIGELGQTITD
                     IDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDIPD"
     gene            22069..23367
                     /locus_tag="VXM85_000580"
     CDS             22069..23367
                     /locus_tag="VXM85_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV1345206.1"
                     /translation="MSQTYQIKQSDIVIDLPKTVGAGYGQFWRSRNLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWGNLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITVKATGQKILFRGLDDELKITSITVDIGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            23379..24848
                     /locus_tag="VXM85_000581"
     CDS             23379..24848
                     /locus_tag="VXM85_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1345207.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITMHNTESSHLKKLKSYYL
                     GEHAILEHKRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTAEGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMAELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            24757..26394
                     /locus_tag="VXM85_000582"
     CDS             24757..26394
                     /locus_tag="VXM85_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEV1345208.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEV
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPESEIKHLQSFSWVGDGSTYSTDIWKNTGKLTSSIKDELLMSLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFVAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKVEYVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKIIADSLVENISLSKAVSKINDALRYTTIFDSDTFTEEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            26396..26605
                     /locus_tag="VXM85_000583"
     CDS             26396..26605
                     /locus_tag="VXM85_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345209.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            26602..27015
                     /locus_tag="VXM85_000584"
     CDS             26602..27015
                     /locus_tag="VXM85_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1345210.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFVSGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
     gene            27058..27345
                     /locus_tag="VXM85_000585"
     CDS             27058..27345
                     /locus_tag="VXM85_000585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345211.1"
                     /translation="MNKYKKLIELIENNGLEIQSKKCYDPQSAWHGEELWIVDKKKQN
                     KIFDLSGNGYCFHDAKVEEAIEEVEKYLLLKKMDTFDDFKKWVEKNAKPKK"
     gene            27486..27698
                     /locus_tag="VXM85_000586"
     CDS             27486..27698
                     /locus_tag="VXM85_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345212.1"
                     /translation="MEEWKERFKKEYYELKERFQKLDMMIGKYEKGQLEFESKCPIDL
                     LKGQRSTMWNYLRILEQRAKIEEIKL"
     gene            27868..28452
                     /locus_tag="VXM85_000587"
     CDS             27868..28452
                     /locus_tag="VXM85_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV1345213.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDSVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPV
                     DSDGITKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            28459..29304
                     /locus_tag="VXM85_000588"
     CDS             28459..29304
                     /locus_tag="VXM85_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEV1345214.1"
                     /translation="MTAGQTKLATMVNPEVMADMVAAKLPKLIKFTPLAYVETKLEGQ
                     PGSTLTVPAWEYAGDATEIEEGQAITPDQLTTKKTTMTIKKAGKGYEITDESLLSGLG
                     DPLGQATYQLGLAIANKIDNDLVAVAKTATQHITETPTTLEAIDKALDIFEDEEDAQY
                     VAIINPKDATKLKTAVAKEWIKGSELGANMVVSGTFGEVDGVQIVRSKKVDEGKGFLV
                     KVSPSQTQTDDANKYGAFVILLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGGA"
     gene            29316..29498
                     /locus_tag="VXM85_000589"
     CDS             29316..29498
                     /locus_tag="VXM85_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEV1345215.1"
                     /translation="MSLLLRRHYIQEEQVNQYSDLENKTLEELKDLAKEAGVAGAYKL
                     TKAEIVEVLEELKSEI"
     gene            29488..29676
                     /locus_tag="VXM85_000590"
     CDS             29488..29676
                     /locus_tag="VXM85_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345216.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVT
                     ISDVLEKILPEPEFLEED"
     gene            29678..29989
                     /locus_tag="VXM85_000591"
     CDS             29678..29989
                     /locus_tag="VXM85_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV1345217.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            29970..30308
                     /locus_tag="VXM85_000592"
     CDS             29970..30308
                     /locus_tag="VXM85_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345218.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            30283..30798
                     /locus_tag="VXM85_000593"
     CDS             30283..30798
                     /locus_tag="VXM85_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1345219.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNAAIKVVQADAR
                     RLVPVNDGELITSIKTRAKMDGDRAIGEVYTNLKYAPYVEFGTGPKGQASHSGISPEV
                     SVTYKSHPWYVHEDQINVGSYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIQ"
     gene            30795..31163
                     /locus_tag="VXM85_000594"
     CDS             30795..31163
                     /locus_tag="VXM85_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345220.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDKEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            31169..31582
                     /locus_tag="VXM85_000595"
     CDS             31169..31582
                     /locus_tag="VXM85_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1345221.1"
                     /translation="MLANGITLSYSKTKGSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYELGIGDVGELEYKFSYNNSSATAPYRVLRKAADDKEKLYFEQAYPDGTKVTFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSELEFADGVGG"
     gene            31587..31967
                     /locus_tag="VXM85_000596"
     CDS             31587..31967
                     /locus_tag="VXM85_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345222.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSLEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVVQ"
     gene            32006..32356
                     /locus_tag="VXM85_000597"
     CDS             32006..32356
                     /locus_tag="VXM85_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345223.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKVFEFWEYAPELFVEEQQAVEQERQRQALLLHKERMRDFAE
                     RHNRKRKEEVNGNS"
     gene            32343..35948
                     /locus_tag="VXM85_000598"
     CDS             32343..35948
                     /locus_tag="VXM85_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345224.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEAARYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIKSTEAFKRFSNGQSWDQLDFQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSIATIGLGIGVFLAESIANG
                     LGRQKERIIKALVALFDNIGNIAEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKDFEKVVVTNAPKISSIFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVAPAINSIANAFNGLIDIIQILWEGSWKPFAEFLSNTFGISIETVADL
                     LGGIILEALKLLADTIKLVADGFTAFSDWCKENKEIISTIASVIGTLATVWQGIKFLS
                     WAEQAGGLAGAFELLSSKVSFIVSGIKNLGLALKALTFDKLVSFGETIYLNALYAKDF
                     VVNSGKTIAQLGKTALELGKSALAWTAHTAKMGLATAAEFAHSVAAGVATAATWAFNA
                     ALAVLTSPITLVIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICRAIGEF
                     FSGLWTKLQEIFEPIGQWFGEKFQQAWDAIVNIFTPIGSWFGQRWADVTSALANIGAW
                     FTDMFQKAWTGLTNIFSKLGLWFGERWADVTSVLANVSSWFGNMFTSAYNAVKNAFSS
                     IGGFFSGVWSTVQSIFVNAGQKVGSAVGGAFRSAVNGVLGTIENVVNGFIGMINGVIG
                     MINKIPGVSLGGIGYVSLPRLARGGIVDSPTIAMIGEAGKEAVVPLENTGFIQTLGRV
                     VSSAVVNAMAGISPQGGFSGDGDIVIQIAGHEFGRVAIQEINKEHERAGQTLLKI"
     gene            35957..36307
                     /locus_tag="VXM85_000599"
     CDS             35957..36307
                     /locus_tag="VXM85_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345225.1"
                     /translation="MAQLTINGVAVKPPKSFKVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSEFFEVSYPDPMDGQVTKTFYVGARTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            36312..>39088
                     /locus_tag="VXM85_000600"
     CDS             36312..>39088
                     /locus_tag="VXM85_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1345226.1"
                     /translation="MDALTRRQFDRAMFAKNRTLAIRVGDYASQDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVLEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQISGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGVEAKVQAQIEAADKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKEAKRKAEEALRNAGASTLLAQEAKRIGLDSVARLEAFKSQ
                     TTSAQTALSGDLDALKRTIVNDIRPKQAQAETEIAKQVEALSRTKNELAGASTLFAQE
                     AKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEA
                     LSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELSSKIASVQA
                     SGRNLFLNSLFKQDISKTGIWTTSTYTAAIDSESKYLGHKALKIIGLNPSGRDGGNPK
                     VTYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIK
                     RYVVHIPKGWTNESKQTTNEWLFNFNQEGTIWIWMPKFEISDVDTSYSEAPEDIEGQI
                     STVESNFKQRADSLEAGVSRLTEGLRTKADISALNVTAENIRQSVKSLETDTQNKLNQ
                     KLSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKMKVGGRNLWIKSKT
                     VGAVIEKLPEN"
BASE COUNT        12760 a         6847 c         8924 g        10557 t
ORIGIN      
        1 gtcctgcgcc tgaggacgct gatggtctca tcactgaagc taaggctacc tttgagcgga
       61 cagctcaggg cttgcgaacc gacttatcag ctattcagga atatgtaaat aaagacggtc
      121 agcgacagga agccctacag cgctatactc gtgaggagag cacgagacaa gcgacagcag
      181 tccgtgagct ggtcaatcgt gatttcgttg gtaaggctac ttatcaagaa gatgttaagg
      241 gtatcaatca gaggattgaa gctgttaaaa ctagtgcgaa taaagacatc gctagtcaaa
      301 tcgctagcta tcgtcaatct gtagatggta agttcacgga tatttcaagt cagataacta
      361 cttataagca agatgtgggc ggtcaaatca gtggtctatc aaatagactt acaagcagtg
      421 agcaaggaac cactactcag atttcaaata tttcaaatcg gataaacagt aataagcaag
      481 gcacagataa tcagatttca aatttaaaga ctcaggtcgc tacaaacaag gataatgctg
      541 aacgacaaat gggtagaata tctgatcagg tttctgcaaa caaagcgaat gctgatagtc
      601 aatttgcgaa tgtgaccaat caactagcgc gaaaagtaga gactactgac ttccagcgtg
      661 ttaaggaaac cagtaaactt tacgagcgga ttttgggcaa tactgaaaat ggaattgcgg
      721 ataaggttgc tcgcatggct ctgaccaatc aactgtttca ggttgaggtg gctaaaaatg
      781 ccagcaatgg tcaaaatttg ttgcaaggca caaaagactt ctcagggggt tggaaaaaca
      841 agggtgcaaa ttggaaaaag cacgctgaaa aatacaaagg tgttgatgtc ctatttaaaa
      901 ataattcgtg gaatggcgtt ggacaagaga ttgatgcaaa aattggtgaa gtctatacat
      961 tcagcttatg gatgaagagc gactggaaga atgatacagt aaatttctat gtaaatagaa
     1021 atggatctgt tgagaaaggt tggggtgttc catctgaaac atcggtcgct ataacaagtg
     1081 aatggaaacg ttactcattt accttcaaaa ttactgtgga cggcttcatc tttcctcgtg
     1141 tagaacgact taatcaaaat acaaatctat atattgcagg tcttaaactt gaaaaaggat
     1201 cgtatgcaac accgtacacc gaagctcctg aagacacgga cgaagcgatt cgctctgttc
     1261 aaagccaact aactggctca tgggcagttc aaaacatcaa ctcggctgga gatatcatct
     1321 ctggaatcaa tcttggcgcc aatggacata accgcttcgt tgggaaattg acccacatca
     1381 ctggagagac cctgattgac agagcagtca tcaagtctgc catggttgat aagctcaaaa
     1441 cggccaattt tgaagctggt tcggtcacga ctacgatatt agaagctgaa gcggtaactg
     1501 ctgagaagtt gaaagttgac aatgcgctta ttagaaaatt aactgcaaat gatgctttta
     1561 ttgaccaact gatatctaaa cgtatcttct ctactaaggt tgagtccgtc atttctagct
     1621 caaccttcct agaagcctat caaggccgaa tcggtggatt cacacttggt caatttgacc
     1681 agggtggcgg tcgctggatt tcaggtgtca atcagttctc tgttggtatg gggaatggtg
     1741 ccgggcatgg agtccggaca gccttctggg cgaactgggg aaataattgg aactatgccg
     1801 gacctaaagc atggaacgtc aatactgatg ggaaaatgta ctgtaggaat gaagtcggtt
     1861 tttatgatca agtggatttt tcgaattcat cgagagcaaa cttctatggg aatactactt
     1921 tttctcgttc tcctgtgttt tcaaatggta tcgaacttgg aagtaaagat gtgcttggtg
     1981 atggttggaa tcccaaaggc ggaaggaatg cggttgtttg gtggaatcag gtcggtagcg
     2041 gtagcgtgaa gtattggatg gaacaaaaat cagacagacg cttaaaagag aacatcacag
     2101 atacagctgt gaaagccttg gacaaaatca acagattaag aatggttgca tttgatttca
     2161 tcgaaaataa aaaacatgag gagattggtc taatagctca agaggctgaa accatcgttc
     2221 caaaaattgt ctcacgagat cctgagaatc cagatggcta tctgcatatc gactataccg
     2281 ctttagttcc ttacttaatc aaggctattc aagaattaaa tcaaaaaata gaaaaaatgg
     2341 agaaaacaat agcatgaata acaacatgtt gaccaatatc gcacttaaag caattcagga
     2401 gcttgctctt gaaaatagaa aacgaacaca cagattggag aacttagaaa atgaacacag
     2461 aacagcttaa ccaagcctta caaatgacaa ttcgtgaaat gtcaacaact tcaacagatt
     2521 cgatgattac aagtaatttc ttgagtattc agttgaatga gcaaagggaa gagaatcaaa
     2581 gacttcaagc acgagtggat gagctggaag ctctgcttga tgaacaaact aaaccagcag
     2641 acaaaggaga atagacatgg cagaaacaaa tcaaaacaca gataacttac tagaccttac
     2701 aaaaatcaca gaaccatttg atcttgcgag tgctttgcgc tacatgaaag aaaatggaga
     2761 gttcattcgt tgcaagaatg taagcgatga cttctatatg tatcgtgatg ttcaaaaacg
     2821 tcctgtgatc gtaaatggcc gtcgccaact caaggatgtt gaaaccgttt gggcgttcaa
     2881 ccagtggggt ggtacaatcg caacaatcaa cgtagccgtt ctgttgaatc atgaattcta
     2941 tatcatgaaa tttgatgcag agggcaatcc tgactggacg gatccaacgg taaaatctaa
     3001 agaataggag gtgtgtatgc caattgaaga agctgaaaaa atcgctcaaa gtcaggtagc
     3061 ttgggcgatt ttgtttatct tgcttttctt tattatcatt cgatatctta tcaagacttc
     3121 ggacaagcga gagaagaaga ttatggattt gcacgagcaa tcaaaggccg actctaatag
     3181 acgagaagag cgtttgatga ctcacctaga aaagaccact acagaattaa ccacaatcac
     3241 tcacgcggtc ggagacattc aaaaagaaat ggttcgcatg aacgaccgca tggaagaaat
     3301 cgaaaaagga gaataacaaa tgcaacaaat tactgaaatc attactaatg gagcaatcag
     3361 catccttgtt attttggctg gtatcgcagt caaggcagtc aaggactacc tggttcaaaa
     3421 aggtggagaa aagactatca agattgttga aatcttggct aagaacgcag ttaatgccgt
     3481 tgagcaagta gctgctgaaa ctggctacaa gggagatgaa aaactggcac aggctcgcgc
     3541 taaagtccgt gctgagctta caaaatacaa tattagcatg actgacaaag acttagacac
     3601 cttcgtagag tccgcagtga aacagatgaa cgacgcttgg aaaggataag agtaatggat
     3661 atcgatacaa gcagactacg tacaggcttg cctcaggtcg gtgtgcagcc ttatcgacaa
     3721 gtacacgccc actcaacagg caaccgtaac tcaacagctc aaaatgaggc tgattaccac
     3781 tatagaaagg accctgaact agggttcttt tcacatgttg tcggaaatgg ccgtgtcatg
     3841 caagtaggtc ctgtgaacaa cggaagttgg gatgttgggg gcggttggaa tactgagagt
     3901 tacgcagcgg ttgaactgat tgaaagccat tcaactaaag aagagttcat gacggactac
     3961 cgcctttata tcgaactctt acgcaatcta gcagatgaag caggtttgcc gaaaacgctt
     4021 gatacaggga gtttagctgg aattaaaacg cacgagtatt gcacgaataa ccaaccaaac
     4081 aaccactcag accatgtgga tccatatcca tatcttgcaa gttggggcat tagccgtgag
     4141 cagtttaagc atgatattga gaacggcttg acgattgaaa caggctggca gaagaatgac
     4201 actggctact ggtacgtaca ttcagacggc tcttatccaa aagataagtt tgagaaaatc
     4261 aacggtacct ggtattattt cgatggctca ggctatatgc ttgcagaccg ctggaagaag
     4321 tacacagacg gcaactggta ctggtttgac aactcaggcg aaatggctac aggctggaag
     4381 aaaattgctg agaagtggta ctatttcaac gaagaaggtg ccatgaagac aggctgggtc
     4441 aagtacaagg acacttggta ctacttagac gctaaagaag gcgctatggt atcaaatgcc
     4501 tttatccagt ccgcagacgg aacaggctgg tactacctca aaccagacgg aacactggca
     4561 gataagccag acttcacagt agagccagat ggcttgatta cagttaaata aatagaaagg
     4621 aaactttcta aattgttctt tcaccgcagg ctcaggcttg cggttttttt gtttgtctga
     4681 aaattgactt gttggcgtca acaaatagta ttaaatcgct tggttgccaa ttttgttgac
     4741 attaacaaaa ttgctctgaa agtactgtct gaattaaaaa agtaatgatt ttttcataac
     4801 tttttatctt cttttacgaa tagataagta ggaggaataa aaatgaagat tttaaatatt
     4861 aaactagcaa atgtagagca gacagattca ggctttgagc attgggtgga tgtgacttac
     4921 aacgtcccaa ttttgaaaaa cgaatacacg gttagattgt tgctgctctt tggttttgaa
     4981 attgaagatc ctgaagtgat tgaatacctg gtcaacgctt ggaaatatcg tgagctggtc
     5041 ttacattctg tgaggatgta tgatatggag agagaagcaa gttaaaatac tgttactaac
     5101 tctaacttat cttaattact ttgactaaaa tacagaaata cgctagaccc tttaaaaatc
     5161 tagcgttatc gacatgccgt cttttgtaac aataatttct tttatgatat ttttgacaat
     5221 ttttgaagca tcttcgtagc ttaatttttc aacatcgaag tcctttagta gccgtgttag
     5281 ctttctcttt cggatgctca gaacattcga tttttgattt tcgagctgtt cttccaggaa
     5341 ttgcctttct gttttgattt tatcattttt ctcgtccaac tctttccgag tgatgatttc
     5401 gtccaagtac aattctgtta attttgagac ttgattattt atccgttcga gttgtttctt
     5461 tatctcctcg accttgatag tctcttcttt cttagcgagt gtctcatttt tatattttgg
     5521 atcaaattta attctttcaa gttccttgat tacaatcctt tcaagttcct ctttagagta
     5581 ccaccctgat tcgcatcttt tttctaaatc tttgctgtgt cggttcctgc attggtattt
     5641 atgatgagag attccattct tgtcttttct tgtatatctc agaccaagtg aagcaccaca
     5701 atatccgcat ttcattaatc cagaaagcat atactttgcc ctgaacggcc ttggattgtt
     5761 atatttttca taggctgaaa tctgccttct ttcgacttca agctggacta agtcaaacaa
     5821 ttcttgagtg ataattggtt catgctgccc ctcgtatacc ttatctttgt actttacttt
     5881 tcccaaatag gtttcatttt tcagcaagta tttcgtgatt gtttcgcccc aaggtctttt
     5941 tcttccaacg tgaccttcag catttaagtc tctgatgatc ttgactactg gcttaccgtt
     6001 caagtattcc gtgaaaatac ggttgacaat gagcgcttgg gtcggattca ctgataagac
     6061 gccagtttct tttgagtaat catagccgaa tggtattgtc gtccatgaca tggattttcc
     6121 attctttgcc cgtccttctt ttcctaacat catacgctct ttaatctgct cacgctccag
     6181 ctgagcgaat actgatagca taccgattga agccttgccg aaaggcgtag aagtgtcaaa
     6241 gttttcttgc aagctgataa atgccacatc gtttttagca aacacatctt caatcagaaa
     6301 cagagtatct ttctggctac gactgaggcg gtctagcttg tagactagca ctatatcgac
     6361 tttcttgcgc ttcacgtctg agattaaacg ctccaattca ggtctttgag tgttagcacc
     6421 tgagaaacca ccatcaacgt agacatcgta gattttccag tctttgattt tacagtaggc
     6481 ttcaagcttg tctttctgct catcgataga gtaaccctct tccacctgcg aagtagtgga
     6541 caccctgacg tagattgcga ctttattcgt tgttttcatt gaatttgtac cccttttttg
     6601 ataaaatagg tacaagaaaa cgtctcaaaa ggtaatatct tttgaaaagt ttttcttgcc
     6661 actagcctca cgctcggagt cgccaaactt tgagagcgtg gggctttttt tgtttgcaat
     6721 tatttccatt ttggaaacag ttggttttat tctttcgata agtgttgttg aagaattaag
     6781 gccacgttgg ctttctcttc ctctgtcata ggaggttcgt ttggatcgtc taccgaaaac
     6841 tcgatagcat gccatttatc atttactcta atccactctc ttcgtctgtg gcatttgcaa
     6901 tctaggttgt gtttaatcac ttccatcggt ctactttcgt tactcatgtt atttctccct
     6961 atacaaatcc acgacttcgc cgataattcg gaagtcggtg tctggtgtaa ttggcatatc
     7021 tttatattca gggtttaggc tatgtaggta agcctggtct tcatcaataa caagctgctt
     7081 gatataagca tcgccgttgt agttgaatac tccgataact ccgtcattca aatctacact
     7141 tgtctgaatg aataccaggt caccatcgtg gtagtcaggc tccatggagt cacctttgat
     7201 ggggatgaca aagtcagcat caacatctac tggtaactca atccgttcca ctcgtacatc
     7261 attcaaatac tggcctgtac ctgcggaagc tgggtggtcg tagtagtcgt aactgtagag
     7321 ccgaatagct tctgatactt cgtttatctt cgtttcttct tcgttttttt gctctttcag
     7381 ttgcctcttt gcataattca ggactttgcc ctgtctaggt ggttctagtt cgtcgtagat
     7441 ggtttgaatt tggggagtat tagaagaaga gtcattgacc atgaaattat tagttataaa
     7501 tctaggatct aatacagatt taggaactcc aaaaaaatct gcaatttttt gaacattacc
     7561 agggattggc aaagaagttc cttttacata ccctgtcaat gtactaggcg gtattcctgt
     7621 tgctcgagac agctcagctt gtttacaatt tctatcagat aaaattgagt taagatttgc
     7681 agaaaagact ttcatatcct ctttatcttg aggagttaat ttccctcgtc ctcttgccat
     7741 atttttacct cctatcttct ttactatata ataccgttta ttttcgattt tgtaaataaa
     7801 aaattcgaaa aaattacgaa aaaattcgaa aaatcattga catacgattt aaatcgtagt
     7861 ataatataat caagcttaag gaaataacaa aaacaaaccg gagggaaaca ccatgaacac
     7921 attaaacgag aaagcaatca acatctttaa agcagtagct aaggaaactt taatccaagg
     7981 cacttacgag gaaaacttcc tttacagcca gcttgaagca ttctgtacta actgccgtca
     8041 attcgctttt ggatggacag agttagcaga ggagattgaa cgccaagagc gttaccttct
     8101 cgattctggt ttcactcaag aggaaatcga tgacattcgt tttgatgcag catttgcagg
     8161 aatgcttgat aaattaaatg tagcctgatt ggtatcacca aggttcgaat ccttggcagg
     8221 ttgttgctca tagagcgaaa aaagagagaa aggagtagaa cgatgaatga atcttttctt
     8281 acgatactag gcatatcaat gattgctagt tttatcacga atttaattgc ttacttagct
     8341 ggtaagcatc atctgaaaaa gaaaattaaa aaccacaaaa tgtggtttga ttctgagata
     8401 gagcgtatca agaaaaagta tcatttgtga tttttcttgg ataatttttt cacaaattgt
     8461 ttttgaagat cggaaggttg ttgcttgttc gcgaaatgac taagagtgct catatttttt
     8521 attgctacat ctgcagatat ttctcctgaa atagctttaa aaattaggtc atttatcttt
     8581 aagccttggt cggtttgagt gtctagttga gatacttttt caagttcaag taatcttaat
     8641 tcgtgagttt gttgcagcga ataaagttct tgtgagtgtt gcttttgcat tttttccata
     8701 tcttgtttaa attgattttc gacattttca agctcttgtg agtgtgcaat gttaatttta
     8761 tcaatttcat gcttgctgtt tcctttagca gcgatgtaag accacatgcc agaaacaaag
     8821 gcaggtatgg cagcgatcgc aagtgtttct ataaaactaa aattattcat aagatttctc
     8881 caatcatttt attttgatta taccacattt gaaaggtagt tagaattgga agataaaatc
     8941 atcgaacttg ctgattattt catcagcgaa aacacaacgt acagagaagc taaaatagcg
     9001 tgtgagaagc tatttcttca agtcagtcat gagatagaac tcagggcaat ggaaagtagg
     9061 acgagggtat gaaagaaaaa ctaaacgaat ttctaaaatt cagaagccag tttacaaaac
     9121 gtgaatggtt tgaaatcaac caaactgtcg aagctcgttt aaatcaaaaa gccgaccact
     9181 tgaaactgga cgacgtagat ttagaaatca tctctaaaag actaggacga tctatctaaa
     9241 gaaagggtga aacaaatggc aactaacaga actatatcag taaatacatc agagcatgat
     9301 gtattgttga cggcaagaaa aaaccaccct gctgtattcg tcgatggaat gtttctcgac
     9361 ggagttgagc gagtggaatt taccaatcat tatctggaga agtgtgaagt tgttttaacg
     9421 tttaacgata gagttgaaac caatcccttc cctctaaacg atattacttt attagaaaag
     9481 ttatttggtc agagttcgaa cggtcaatct ttacgggata ttgtcgtgca aactcttgaa
     9541 gatgctgatt agcatctaaa ccatcaaaaa aagaaacatg aacactaaaa ctttcttttc
     9601 cgtttttctt ggttctatca aattctttgc caaggacaat tagagaagct tctaattgat
     9661 aatcagtcat aacatcacct cctttctagc tttattatag cagaattgcg aggaacaaat
     9721 agaaaaataa ggaggtagga acgtgccgaa aatgacattg agagcaataa gaacaaatta
     9781 taacttatct gcaaaagaag ttgccgataa acttaacatt catcaacaaa cactgttgaa
     9841 gtatgagcat gatagttcaa aaattccaat ggatctttta gataaacttg ctcgactata
     9901 caatgttgaa aaggatttta tttttttagg caaaaaatac gaattaaatc ataatctagg
     9961 agaggtgtga atgaacgagc gagagttata gaaaggggat taaatatgag gtatgcagta
    10021 tataatcagg aatactcatg ggaattacac atctttgaat aatgcttatg ctcaagacaa
    10081 acgtttaaag gcaacaacga taggtatcct tacagtaatc ttgatgaata agtctgattg
    10141 ggttgtgtat cctgacgaga ttgcacgacg tctaggaata agcaggcgca ccgtagatga
    10201 gcactttaag cttttagaga aagcaggcta tctcagagta tatcgcttag ggttaggcag
    10261 aggtaaaggc gtaacggtat atagattttt ttcagatatg cctatttcag atgattactt
    10321 tgagtatcta aaaactaatc ttgagaaaga gttatccaca gataacggag tttaaaaata
    10381 cagttggaaa atattgccat gtgtaaaatt gccatgtgta aaattgccat gtgtaaaatt
    10441 gccatgtgta aaattgccct ctaataagta ctaactatac aacaagtact aactatacaa
    10501 taatctaagc ctaacggcac taacttagta ataactacta acttacaaca aactaatact
    10561 tctctaaaaa ataaaagaga gaaatttcaa ttttaggact ttgaaaaata ggaaaggaga
    10621 aaaaatgaga ccaagacgat atccgtatag tgggaaaaaa gagtccacct ttgtaaaggc
    10681 cgaccctgag ttagttgaaa aacttttaag aaacactagt tttcttgagt gtttacaaaa
    10741 aaagcctatc aattttcaga tagactcaga agaatttaag cgtcttagct atgaagccat
    10801 tcatgatact tctcaagtaa ctcaatagta gttattacag aagtcaaacc actgaccttc
    10861 cccagttgca atccgtctgt atggtcaatc tgtttagtag cttcattagc tttagcagag
    10921 atagcttgca tatcttcagc tgttaaagat tctcgaaaat ctttaaagga tttcataaaa
    10981 actcctcctt tctattggaa ttttgactaa aacggtgaga ggtcctagtc gagattatta
    11041 tagcaattta ggaggatatt acatcggtct tgaggctgat ttttggaggc aatattggaa
    11101 gataaaatca tagaacttgc tgactacttc atcagcgaat ctacaacgta cagagaagct
    11161 aaaatagcgt gtgagaagct attgaaacaa gtcagccatg agatagaact cagggcaatg
    11221 gaaagtaaga cagtctagaa gacaacaaaa agcacctgac ggcaatcagg cgcatactaa
    11281 aaaactttac aagaggatta taacatgaat atgaacttaa atacaaatga aattttaaca
    11341 actactgaat atgatatgtt tcgcaaaatt agtaatagaa aaataactga aaatcctaaa
    11401 ttggaagagg agctactttc tgagggacaa cgtcagccga ttttggtaaa tgaaaaaatg
    11461 gaagttatcg acgggcaaca tcgtctttat tacttgagaa aacatagaaa accagtgcgc
    11521 tacataattg atccgaccgc taatttcaag acagtaattt cgatgaatac atcagctgtc
    11581 aactgggcat tacaagatta tgtgtattcg ttcgctttag agggagatcc tgaatttgtt
    11641 aaattagcta aatttttaga cgagaacgaa ttgcttagtg acaagatggt aatcgtagct
    11701 ggttcaggaa gacgtgatgg tacggcagca cacgtagtca aaaaattaaa aaaaggcgat
    11761 tatgtatttt caaacgaaaa acagttaaga gaattttgta agttctacga acgcgtttta
    11821 aacgaaacaa aactgcctaa caaaccattt ttacaatctg ttttatggac tttgtataca
    11881 acatcagttt ttgatgaaaa cagaatgttg acacaattga agaaatcaga tttgacatca
    11941 gaagatatcg aaggatttgc aaagaaaaaa ttgctattaa cttttttaga gctatacaac
    12001 ggaagatgga gtgacgatca tccttcttta attcaatact ttatcaacag aaaagggtcg
    12061 ttaacaattc ctagtttgcc taaacaagat gaagataatt aaaaaagtag gaagataaaa
    12121 tgaaagtcac agtatatgtt tacggccgaa aattagaacc agatgaacca attatcatcc
    12181 cagaaaatca tcgtttttat gacatttgga acggaattgt aaatgaaatg ctcgacaacg
    12241 aggaggatgt agcttaatga aactacttac taaattaaaa ctcagactcg aaggagttct
    12301 taaatcagtc aaccttgact ggagagaggt agcagtcgaa cttatgaacg acctatttga
    12361 ggagcgcaag cgtcgctttg ctttcgagca agaaaactat gacttgaagc aggagcttgc
    12421 tgcctacaag tacaaagaaa actttgatat caaggctaga ctgcaaggag aaatgtagat
    12481 gtacattata tcgatttatg tcaagaatac tgaaactgga aacgaggatt tcagtttgat
    12541 tggacgtgat ttcttaccga cggggcacca agactatatt gcaagagttt ttggaacaaa
    12601 agaagaagcg attgattact taaaatctat atcttacatc gcatcaggtg ttcatggtaa
    12661 cgattgggtt tatcaaaatg aaaaactacc agaaattgag tcacgttgcc gaatttggaa
    12721 agtaggagaa taaaaggaga acaatatgtt taaagcacta aaaacaatca aaaaaatcaa
    12781 acaacttcag aaagaaatgc acgctttcag ccttgcgttt ctagctctac aagatatggg
    12841 cttgatgcca gaaactgaaa gaagtaaggc aaaggctcaa acaatgcacg atgtaagccg
    12901 cgtactcaag gacgtcttgg acggcaagtc ggtagatgaa gcgataaagc gtctaaatag
    12961 cgaagtgaaa gctgaagagg tggggcagaa agatgaccag aattgaactt gaaaaccgtg
    13021 tatggctttt ggctaatcat gaagaaaaaa acgaattgct ggatcttggg ctaacgtcaa
    13081 agaccagata tgtgaagcga gttctggaac taggaaaggt gtatgctcat gtttgattat
    13141 gacagagata taatgcaacc gcctgaagaa cgagaagaac gtgacccagc tgattggatt
    13201 ttcagcgctg gtcaatggat ctatgtagga gattgttagc ctatgaatag agaacacaac
    13261 gaaaggtaga aggaaatgac gaataatcaa attgtagaag caaaaggcga ctttttaacc
    13321 aacccacagc tacttaatag cggtattatc agaaagtatc ttgacccaca aggaaaagct
    13381 agtgatgagg agcttgccta ttttatagct caagccaaag cccaaaacct caatccattt
    13441 acaaaagaaa tttattttat caagtatgga actcagccag cccagatagt cactgccaaa
    13501 tcagcttttg aaaagaaagc agatagtcac ccgcaatttg atggcaaaga ggcaggcgta
    13561 atctatctga tggatggtga aattaaatac tcaaaaggag catttattcc taaaggtgct
    13621 gaaattcttg gcggttgggc taaggtgtac cgcaaagacc gtacttaccc aacggaaaca
    13681 gaagtatctt ttgaggagta cgacaattct aaaatacgtg caagagttaa ggaactgaca
    13741 caacaaggta aagatgttac ttatccagtg atgaactcat acggtaagcc aataggtgag
    13801 aataactggg atactatgcc ttgtgtcatg atacgaaaag tagctctagt gtcagcttac
    13861 cgtgaggcgt ttcctgccga gcttggagca agctatgagg ctgacgaaat tcagctggat
    13921 aacacaccta aagacgtcac tcctcaagaa agccgtgagg atgtcgtagc acgcaagatg
    13981 gctcagattg agcaattcaa caaagagcaa gaggcaaatc atgcagatcc tgaacctgct
    14041 aaaactgagg agccaatcca gggcgagttg ctagacggtg aacttgaata ttaggaggac
    14101 aacatgcaag aattacaggt tattgatgat aagaaaatca ataaaatcta tgagatgatc
    14161 acaacggatg aacttactag agagtctttt gaaaaagacc tcatagaggc tactgagaag
    14221 tacaaggact atattcctac agctggaact ctcaaagacg acaaggcaaa gcgggctgaa
    14281 tttaacaagt taattgagtc taaaaatcgt atccgcattg acactaaaaa cttactatca
    14341 gagacagcta acacatggga tagttatgct aagtcaatta ttgacccatt tgcaaccgta
    14401 gttagtgaat ttgacaaagg tatcaaggaa atcgaagaac atcaaagaca actaaaaata
    14461 gatacggtta agagttacct agccaacaaa tcggctgagt acatgctgga ccctcgtctc
    14521 tttgatgaaa aggcccttga gtatgtcaag gctggcgatt tcatggcaga cggcgtgacg
    14581 cttaaaaaag ccactatgaa gtcacttgat gacatggtca catttgagtt tcagaaacaa
    14641 caagaatttg aaaaagccaa gtcagctatt tcagggttat gtgctgagta cggcatgact
    14701 gactcacctt acattagaca gctgaaagac ttgactcttg ctgaagcctt tgaacaaatc
    14761 aaagctgatt atgagtttga aaaacaaaag gaagaaatca gacaggctca agaacgagca
    14821 gagcgagaaa gtcaggaact tttagcagcc caacaaacca aacagcaaga acgggctcca
    14881 aaatcaacgg gaaccccaaa ttttgaccca gaggcaggcg aaatcttgga cggtgagcaa
    14941 atccctcaaa atgagcagaa ctctcttaga ggggctgaaa atgacctaaa acgatatacc
    15001 caaaaaatga ctttagaggt gtattttgta gacacagccg aaaaagaccg tttcaaggct
    15061 ggtctaagtc aactcggatt tgattttaaa aagaactatc aagtcagcgg ttatcaacgt
    15121 attgagccat tgactcaggc tgaactcaat gagcaatggg gaggtaacca tggacatcag
    15181 aaaaatatct gacagcgtag ccatctactc ggacggcaag aaattgcatg ttatccacaa
    15241 cctaggggat gagtttatcc tagattttaa ggtgggagag gatagcgtct ggaacctcaa
    15301 tggccaagtc gtagaaatta ttgacatgat tgagcctgtc tttaaagttt tcagcttttg
    15361 ctcaaaatct ggagagggta tgcaacgctt aaaacatgct atcgtccact ttgaaatatt
    15421 tgagcagtac atcagagata atcaggaaga cctgatgatc tggtggcaca atccaggagg
    15481 ggaatatgat taaaaccgta tttttttcat gtgattatcc acatcatgag gcgattgacg
    15541 accaaataaa tagctggctt gccgaaaatc caggcattaa gttgattgac atcaaatttc
    15601 aatcaaatgt gtctgctgtc gctgacagtg gagtcagtgc tgaatattgg catacatccg
    15661 cattgattat ttacaaagtt ccctcagaga acaatataag cagtattaat tcaaatggtt
    15721 taggtttcat aatcagctgt gagaaatgtg gtagcttatc aataatcaag ggaaaagatg
    15781 taggtcaaaa tgtatgttat gaatgcaaag gagagaaata atgaatgatt ttatcaaaga
    15841 gattgggatg gctatcctat ggatgttttt agggtatctc ttgggagagc gtagcactag
    15901 agggggacaa atcagatgat caataacgtc acactggttg ggaggcttgt agcgcctcct
    15961 gatctacgaa aaacgcctaa caatgtatct agcttgcagg gcacgcttgc agtcaatcgc
    16021 aatttcaaaa acgaaaatgg agagcgtgag gctgatttta tcaattttaa agcttggaga
    16081 ggtacagctg acatcattgc tcagtattgc agcaagggct cacttattgg gatcattggg
    16141 cgcttacaag ttaggtctta cgagaaagac ggtcagcgtc gatatgtgac tgaagtaatc
    16201 gctgagagtg tagctctgct agagagtcgc aacagtcagc acggacaagg caacagtttc
    16261 caaaatggga atagctcacc ttttaccgat cctaacccct ttgacctccc aaatgacggt
    16321 ttgccgtttt aggagggaca gttagtggaa atagataaga ttataaaaaa agatgtcttg
    16381 gaatttatgg aaacaattcc tgataataaa attgatttaa tcgtaacaga cccgccttat
    16441 ttgataaatt ataaaacaaa ttggcggaaa gaaaagcata aattttcaaa tgttatcaaa
    16501 aatgataaca atcctgaatt gataaaagag tatataaaag agtgttatcg aattttaaaa
    16561 gatgatacgg ctatttatat attctgttca tttgacaagg ttgatttttt taaaaaagaa
    16621 attgaaaagt atttttcagt aaaaaatatt attatatggc gaaaaaataa tcataccgct
    16681 ggcgatttag aggcgcagtt tggaaaacag tacgagatga ttatattggc aaacaaagga
    16741 cggaagaaat tcaacggtga gcgactgaca gatgtttggg atttcaagag agtaagttca
    16801 gataaactac tccatcaaaa tcaaaaacct attgaattga taaaacgatg tattgttaaa
    16861 cattctgatg ttggggatac tgtttttgat ggtttcatgg gaagcggtac gacagcatta
    16921 gcagcgttag aaacggatag gcattttata ggaactgaaa tagatgaata ttattttggt
    16981 atagcagagg agcggataaa aaaccacaac gctcaattaa gtttatttga tgaggtatga
    17041 gatggagtgg acggattggg tggattggaa acctgaaacc aaaacggaca ttaaaaccaa
    17101 aattgaaaat gacgggtaca cttttccaca ctatgacaag aaaaacaacg gcgtcaagta
    17161 cgtcatctct acactggaca tcaaacgaga ctgtctaaga cttggagtac cgtttgaaga
    17221 tgtgtaccct ttgcaaacga cactttttta acaggagaaa gaacatggca agtaaaatca
    17281 atgcgacaga acgtattgct atcatcattg ataaacaaaa aatagaggtc gttacgactc
    17341 taaactatga tatgagcatt agctttgata acaaagacac ggctcctaca ctagatgaca
    17401 atggtgacct ttttgaaccg gtctacaagt gcaaagttaa ggcaattccc aaaaatgatg
    17461 tatttttcac ctcattaaca cgagtcaaga gcaacatcaa gacgctacaa gaggttaaaa
    17521 aattctttga gttcgtaaac gaaaacagag aaaatctctt tgagatggca ggatttaagg
    17581 gggctcttga atgaaattga ccctgaacat tgagcctaaa cctcaatcac ggccaaggtt
    17641 tgcaagacgt gggagtttta ccacaactta cgaagacaag gatatgaaaa catggcgcaa
    17701 tcattgccag ctgctcattg ctaatcagta catgggtcag cctatccttg agggagcttt
    17761 gagggcacgg cttagatttt atatcaaacc tcctcagtac atttctaaag tcaagaagaa
    17821 ccaacaggcc ctcctggatg aaattatacc agtaggcaaa aagcctgaca tagataacta
    17881 cgaaaaagcg ctatatgaca gcatgtcagg gatcgtcttc caggacgacg gtcagatagc
    17941 gctacatgat gtaggcaagt tctacagtct aaatccacgg atagaggttg agattgaggt
    18001 catgaaatcc ctgagtattt gaagaaatga ggagcagatg gctgactacg cattatatca
    18061 aggtgatgtg tttgttacgc ttggaacatt agcgcagatc agtagcgaga caggaattac
    18121 tgaaaggatg ttaaagtatt acacttacac atcacaccaa agacgacacc caaacggtag
    18181 ggccgttatt aaaattgagg aggaagataa tgagaattaa gacggaaagt gaagaagttg
    18241 gaagatgatg gaagagttaa agcaaaaagt taatgaagta tacaactgga cggtagaaga
    18301 cgggaagccg caacctccca agcaagattt accacaagcg gtgaaagacc gggcggacta
    18361 tttttgggaa atggcagaag atggtatgac gtttatggga gcgatggaat gcatctttgc
    18421 tgatgaaaag cctacagact atgatttggg agctactaag ggttggttgc caaaatctaa
    18481 ggagtttgat gattggattg gctattcgcc aagcatggct caggtagtta ttgcagttta
    18541 tttgatttat ggaggaaact aagatgaata agcaggaatt gattgagaaa tataaaaaac
    18601 ttgagggtgt atggaatgct gaaggagcag aactagctcg tcaaattttt ctgcaagact
    18661 tggaacaact agataaacca aaaccagtca aagttccgca gtgtgtggcg gaatatatag
    18721 aatttaaaaa gaaaaacaat tttcatgttt acggtgcaat gagagtaatt gaagatcatt
    18781 atgataagaa agttcctgag tggttttacg aaaataacat cgaaaaattc tgtcttgctt
    18841 ggcttgacgg ctacgaggtt gaaaaagaga agcggtattt ggtgacttta aaaaataggc
    18901 agcctttggt caaatcgcaa tcagggagta ctctttattt tagtcaagat ataacagcta
    18961 ggaattataa aggtactcaa aaagaactag aagacgcaaa cttcggctgg gtgtttgatt
    19021 gtccagggat tgagattgag gaggtggagt gatgagcctt acgctaaata gcacaattgg
    19081 agacttagtt ttggcaatcg gagaaattat cgttggttct gatggtaaaa ccactacagc
    19141 gatactggag atacctgatc aaagctttta cttagagatt gagcttaaat tgaaggagga
    19201 ggtcataaat tgaaacgatt catagctatc tggattctgc tatctgctgg attgaatatc
    19261 tggcagagta tctacattaa aaagttagaa gaaaagcgcc cgattgtcgt ctataaagct
    19321 gataacgcag gcgctgagat attcggtaaa gtcgtcgaga aaggacggca tggcaagcta
    19381 tatacgctta ccattcgtga ctacggggtg ttcgtggtta cgaaggacgt gtacgataaa
    19441 gtgaaagttg gggatgaggt gttactatga cagaaactat taaactacca gactattatg
    19501 agcctgattg gaaaaatgca aggtacgggt cgttggaaga gcttaaagaa ttgttgctct
    19561 ttaagcgtat tgtgaaatgg gataaggact ttttgctgct tgaagacggc acaaaggtca
    19621 ctattgaaat gtctgaaagt gattgctgtg cctcagcagg tggggagttc caagatgtat
    19681 cacttgacgc tgtgattact aatattgaaa ttggagaacc ggaagaaatc cccgaccatt
    19741 ggggaactgg ttataaaaac aaagtaacta tcttccataa tcagaaccct gtagctattg
    19801 ccaattgtga agcagagcat aacggctatt attacagcgt atgctcttta gtgattggtg
    19861 atattcattt tccagttgtt aatgcttagg aggatttaac atgacaccaa gatatagagc
    19921 gtggataaaa acagaaaaac gtatgttttt ttcagatgac attcttgcta ttgactacga
    19981 aaacaaagaa atagtgacac aacaagttta ttttgagaat ggtttaccag acgatagaga
    20041 tatctattgt tatgattttg acgaaatcga actcatgcaa tcaacaggac tcaaagacaa
    20101 gaacggcaag gaaatctttg aaggcgatat tgtacgaact actagatttt tgggtagagc
    20161 tgacgaaatt ggcggtttct atgagtatga caaggaattt atagggattg ttaagcagct
    20221 tgagggttct tgggtaattg atacgggcag tgacgcagta tgtttatgga ctgaaattga
    20281 agaaaatgaa atcatcggca acatctatga aaataaggag tttggaggac gcaaatgaga
    20341 ccttgtaaat atccatattc aggaagaaga aaaaaacaag aaacgccgtc gccaatattt
    20401 tctgcacgac caatttttga cgaagttcca attgtagaag aagttaaggt tgagttcgga
    20461 gttgaagcta gtatggggcg catatatcca gaaacgttaa tacatttaga tatttctgga
    20521 tacggaaata gagtgcattc agtacatcgc ttccccggta ttttactgag tgttggtgag
    20581 tcaatccaac taaagatgct tttctataaa agacttagaa attttactac agatcgtttc
    20641 ttgacgttta gagaatctga ttggaagttc tttatccggg acctggtcaa cgaatttaag
    20701 cattaaaaaa agccaagaca ctctctgcct cagctaatag ttctcgcaaa gactattata
    20761 tcacaaagga gacagagagt gaacaaggct aaagagctct tgaaagagtt gcaggatctg
    20821 gacatggaca tccaaagccg tatagacgaa attaacgagc ttgaggcagg tttgctctca
    20881 agccctaaat ggtccgaggt taaagtccaa ggtggacaga ctagaaaagt tgatgatgtc
    20941 tatactcagt tggtagtgat gaaagaggct atagagcagg atactaaaga ggtcattaac
    21001 agaaaacttg aattaggtag aatgatcaat aggcttaaaa atccaaagta cagggcagta
    21061 ttaagaatga cttacatcaa caaaggcacc gctgatagcg tttgttatga tttgaatatg
    21121 agtcgtacaa cctactacag gttaaaaaat gaggcggtct tagctttgga agaagttatc
    21181 taacctcata gtgagcttat gggacttttt ggaacagcac ggttctaaaa atctgttaga
    21241 atggtagtat caagaattga aaagagaggt ctcagaattg gtagatggtt acctgtaatg
    21301 tcagggggct gtaatggcct tggaggttca agtcctcccc tctcctttga gtgtttgtgt
    21361 cccagaatga gttaaatctt ctgggtgggg attcacatat cactcattaa ctcctatcac
    21421 tcattaactt aaaaatggtt gcggaagcga ctggacctcg catgattgcg tagctaatta
    21481 tattccggat aagttataag ctagagggtt tgattccctc agaggtttta aagactacaa
    21541 aaaataaaaa agaagtcaaa atttaatacg cacgcaaggt agtagtcgcc ttgcaagaag
    21601 gtcgcacatc gtgtggcttt tttgattgtt tgaaaggtgg tgatggaaaa ttgagtggat
    21661 tgagaataaa acaaaagaga tttgcagatg agtacatcat ctcaggtaat gcgacggaag
    21721 cctataagaa agcaggttat cgtgtttcta gtgatagagt ggcaggcgtt gaaggacata
    21781 agttactaaa gaatcctaag attaaaagct atatagatga acgactgaaa cagcttgatt
    21841 ctgaaaagat tgcggatcag caagaggtct taggttatct aacttcagtc atgcgaggag
    21901 agacgcaaga acagaccttg ataagcattg gagaactagg gcagacgatt acggatattg
    21961 atgttggagc aaaagacaga atcaaggcag ccgaactatt aggaaaacgt cataggcttt
    22021 ggacagacaa agtagaggca gacgtttctg gaacggtggt gtttgcaaat gagtcagaca
    22081 taccagatta aacaaagtga tattgtaatc gacctaccta agacagtagg agctgggtac
    22141 ggacagttct ggcgctcaag aaatctttat cgtgttgtaa aaggttcccg tggttcgaag
    22201 aagtccaaga caaccgcttt gaattatgtt atccgtcttt tgaagtatcc ctggggcaac
    22261 ttgcttgtta ttcgtagata ctcgaatacc aacaagcaat caacttatac ggattttaaa
    22321 tgggcgtgta atgtgttggg tgtgactcat ttgtttaaat ttaacgagtc tttgcctgaa
    22381 ataactgtaa aagcgactgg tcaaaaaata ctgttccgtg gtttggatga tgaactcaaa
    22441 attacatcta ttacagtcga tatcggtagt ctgtgttggg catggttcga ggaagcatat
    22501 caaattgaga ctgaagacaa gttcagcacg gttgttgagt ctatccgtgg tagcttagat
    22561 gtacctgatt tctttaaaca aatcacagtc acatttaacc cgtggaatga gaggcattgg
    22621 ctcaagcgtg tattcttcga tgaagagacg agacgggctg acacattcgc tactacaacc
    22681 acttataaat gcaatgagtg gcttgatgaa gtcgatatca aacgctatga ggatttgtat
    22741 catacgaacc ccagacgtgc tagaatcgtt tgtgatggcg aatggggagt tgctgagggt
    22801 ttaatctatg aaaacgtgac tgtcaaggat ttcaataaag atgaattact acaagattca
    22861 gctaataaat tatgtatcgg tcttgacttt ggttttactc atgatccaac tgctttgtgt
    22921 tgttcgttga taaatgatac gacgaaagag atttatgtct ttgatgaggc gtataaagtc
    22981 ggattgataa ccaaagaagt tgcgaagatg ataaaggaca aaggttatca tcgctcacaa
    23041 atcattgctg atagcgcaga gtcacggctg attgaagagc tcaggtcaga acatggcata
    23101 tctagaataa aagagagtcg gaaaggtaag gatagtatta tggcaggcgt atccaaattg
    23161 caaggatacg ctatttatgt gcatccagat tgtaaaaaca tcatggatga attttatagt
    23221 tactgctacc agcgagataa agaaggcaac tggttgaata aaccagagga taaaaacaac
    23281 cacttgatgg acgctttgcg ttacagcctt caatgtatcg aaggtggaaa agcaaccgtc
    23341 cgcagacgtt ctgattatgg tctatagaga ggaaagacat gtaccaatat ttaacctatc
    23401 cacgggatgg atatgatgag ggttctttga agaaagacct gatttacaaa ttgataacga
    23461 tgcataacac tgaaagctca catttgaaga agcttaaaag ctactatttg ggtgagcatg
    23521 ctatcttaga acacaagaga cgcaatgtga acgcacccaa ttacaagacg gtagctaatc
    23581 atgccaagga tattgcagat acagctacgg gctattttat gggcaatcct atcaagtaca
    23641 ataacactgc tgaaggtgat atcgatgaac tacttacagc ctttgatggc gctgagattg
    23701 accaagtaga tgctcagaat gctttgaaca tggctatcta tggtcgtgct tacgagtaca
    23761 tctatgctaa agagggtatg gctgagttgg attcaactag tattgatccg gagaatactt
    23821 tcatggtcta cgatgatagt attgagcgga agcctttgtt tgcggtctat tactatgaag
    23881 taaaagacga tacgaaagac actaccaagc accaggctga ggtctttacc gaaaatctgc
    23941 actatcacat ggtgctgaga agtacagatt caggaacaac tcagagcgag gaggcaacac
    24001 ctcacaacct tggtcaaatc ccaattatcg aatatcgcaa caatcacttt gcaattggtg
    24061 actatgagca acaaattagc ttgatagacg cttataattc cttgatgggg aatcgtgtca
    24121 atgataagga acaggctgta gagtctatac ttgtcttgta tggcacgcag ttagcagaca
    24181 ctccagaaga cgctaaggta gcaatgaaga ttctttctga agaaggtctt ttggaattgc
    24241 cgggcgatag tgcaagggct gagttcttga agaatacgct ggacgaaagt gctactgaaa
    24301 tcttgcgtac agctcttaaa gaggacatct acacatttag ccatgtgcct aatttgactg
    24361 atgagaattt cgcagggaat acatcaggcg tagccatgga atttaagctg atgggccttg
    24421 agatgattac taagaccaag gaagcgaact ataagcgagg attgcgtcag cgtattgcga
    24481 tttttgctca ttacttaggc atgaagcaga ttgctttaga gtctcattca atcgttccac
    24541 aattcagtcg tggtttgcct aagaacttgt tagaaatctc tcagattgtg aacaatttgg
    24601 aaggcaaagt gaccaataga cagcttattt ctctcttgcc gtttgtggaa gaccctgacg
    24661 ctgagctgga agccttggaa gaagagaaaa agaagaacat ggaagacatg ccgatgttca
    24721 acaaagacaa cacgaaaccc gaagacgagg tagaggatga agaatcagga gtattgggcg
    24781 aagaggaaag ccaatctgat ttaccagcag atggacaagg ccgaaaagca ggcagaccag
    24841 ttcgataagg tctatcagga agccaagact tacttggata aggaagtcaa taagattttt
    24901 gataagttcc aacgtgatta tggtctaagt caggtagaag ctagacaagt cttgaagaac
    24961 atgaaagaca agaaaaatct gaatgaactt cgtaaagtac ttgaagcgag accgaatgat
    25021 ccaaatatcc aaagactact ggctgactta gatagtccgg cttattcttt ccgtatgaag
    25081 cgtctagaac gtttgagtga tgatttagac cgtatgcgtg aatctatcta tcattcagaa
    25141 aagacaggct cagacgcctt ttatagcgac ctgatgaagg atagttacta caaggctacc
    25201 tttgacctgc agcagcagac aggattagca tacggctttt ctgggcttcc tgagagcgag
    25261 attaaacatc tacagtcttt cagttgggta ggtgacggaa gtacctactc tacagacatc
    25321 tggaagaata cggggaagct tacttctagc ataaaagatg aactacttat gagcctcatg
    25381 acaggccgag atacacgaga aactgcacaa gcaattgctg agaggttcaa tgtaggtcag
    25441 aacgatgcaa gacgtttggt tcggacagaa tcagcctttt ttcataacca aatggaacta
    25501 ctcagctatg aagaagcaga catagaaaag tatatctttg tggccgtctt agacaagcgt
    25561 acatcacgga tttgtcagga gcatgacaat caggtctatg atagggataa ggctgtccct
    25621 ggtgtcaatt gtccgcctat gcacccttgg tgtaggtcta ctactgtcgg atacgatgag
    25681 gacgcagact acagcaagtt gaagcgcaga gcaaggaatc cagagacagg taaagttgag
    25741 tacgtgcctg ccgatatgac ttataaagag tggtatagca agtatgttgc gaaagacggg
    25801 gaaaaggtgt ataatcaaga tacaagagaa gccaaggcga aattttatag cgaacaacta
    25861 ttgtccaaaa tttcaggagt tgagccaaaa attacaagtg atatgcaacg tatcgcagga
    25921 gaaaacaaat tggcaggtct tgaatttagg aagaaaacag ttgagtcgtt atcacgtaaa
    25981 attattgcag atagcctagt tgaaaatata agtttgtcaa aagccgtgag taagattaat
    26041 gacgccttaa ggtacacaac tattttcgat tccgatactt ttacagaaga gtatttgaag
    26101 atgaaacaga agcttatcgc agaaggttat aaaattgtaa aagtaaaaaa cacttggcta
    26161 gtagatggac catacaaagg tgtgaataca gtcgttgaaa aagatggtat caactttgaa
    26221 atgcagtatc atactcagga aagtttcgac ttaaaaaatg gttcattaca tgaactctat
    26281 gagaagtatc gtgatacgaa tacatctgat ctagaacgca tgaaattatt taaggaaatg
    26341 cttgatttaa gcaatgggct tgagattcct aaaaatatag agagggtgaa gtgatatgaa
    26401 agatattaaa tactaccgca caacgacgaa caatgctcaa gtacttcgtt tgattgatgg
    26461 tgtcatgcaa gtttttgaca ttgaaaaaaa gtgggttaat agcatggatt ggtttaataa
    26521 aatctttttt aatgacttta cggattttga agaaatttca gaaaatgatg catttactta
    26581 tattgacagg atggtagcgg catgattgat attgccttgg ctatcgctaa aaaagcacat
    26641 gcagggcagg tagataaagc gggtgttgat tacatacagc atcctctcta tgtggccagt
    26701 caagtcaaca ctgaacaaga aaaagctgtc gctcttttac atgatgtgat tgaggatagc
    26761 gatataactg ctgccgattt attcgtgtct ggcttgtcaa atgaagttgt tacagcggta
    26821 caaattttga caaagaaaaa aggtcaaagt tatcaagaat atcttgggaa agtaaaatca
    26881 aataatttag caagagttgt aaaacttgca gatttgaaac ataactcaga tttatcacgt
    26941 ttgaaatctg ttaccaatac agactacgag cgtgttaaaa aatataaaaa tgcaatttat
    27001 tacttaagca cctagagaaa tctaagtgct tttttcgtgc tcagaaagga gaatctgatg
    27061 aataagtaca aaaagttgat agaattgatt gaaaataacg gtcttgagat acaatctaag
    27121 aaatgttatg atccacagag tgcttggcat ggtgaggagt tatggattgt tgataagaaa
    27181 aaacaaaata aaatttttga tttatcaggt aacggttact gttttcatga cgctaaagtt
    27241 gaggaagcca ttgaagaagt tgagaagtat ctattattga aaaagatgga tacgtttgat
    27301 gatttcaaaa aatgggtgga aaagaatgct aagcctaaaa aatgatgctt agaaaggagt
    27361 aaagacatgt ttatatggga ttgggtatca atcgcctttg ggtggttggt atttttgttg
    27421 ttaatattta ttattatggc cgtaatcagc ggaataatta aaggtgtaaa gaaaggaaca
    27481 gaaaaatgga agaatggaaa gaaagattta aaaaagaata ctacgaattg aaagaacgat
    27541 tccagaagtt agatatgatg attgggaaat acgaaaaagg gcaactagag tttgaatcta
    27601 aatgtccgat tgatttgtta aaaggtcagc gttcaaccat gtggaattat ttaagaattc
    27661 tagaacaacg tgcaaaaatt gaagaaatta aactataaaa attaaccgca tcgaaatcga
    27721 ggcggttttc ttatgctcta accgtatgga atcccgtacg gtttttatat tgtccaaact
    27781 gtgccgatga cattaaaagc tgtactgttc cgtcgccgga cgtaaaacga gattatcgag
    27841 tggcgacgta atcgctggag gacaattatg tcagaagaaa tcaatgcaac tgtatctact
    27901 gaatcaactg agactgtcga cactcaagga aatgttgatt cagtgcagga agaaaagcac
    27961 gaacgaactt tcactcgtgc tgaaatcggt aagatgctat ctgccgaacg ctctaaatgg
    28021 gaagctgagc aagaagccaa ggaaaacgaa gctaagaaac ttgccaagat gaacgctgac
    28081 gagaaacaaa aatatcagtt ggatcagcgt gagcaagaac tagctgaccg tgagaaggct
    28141 attgctcgca aggaattgac cgcagaagct aaagcaatgc taagtgaacg tgacttacct
    28201 gttgagttag taaatgtagt tgatttgaca agcgcagaga cggtatcgca gtctgtcgct
    28261 gtattgcaga aatcatggga gcaagccgtg caaaaaggcg ttcaagaaaa actaaaaggc
    28321 ggagctccaa tgaaacaagc gccagtcgat agtgacggta tcacaaaaga agaatttgct
    28381 cgtatgggtt atcagagtcg aaatgagctc tatcaaaaga acccagagct ttataagaaa
    28441 ttgaaaggat aatagaaaat gacagcagga caaactaaat tagccactat ggttaatcca
    28501 gaagtaatgg cggatatggt agccgctaaa ttacctaaat tgattaaatt tacaccgcta
    28561 gcgtatgtag agacaaagct tgaaggtcaa ccaggtagca ctttaacagt gccagcatgg
    28621 gagtatgcag gagacgctac tgaaattgaa gaaggccaag caattacgcc agaccaattg
    28681 actactaaaa agactactat gaccatcaaa aaagcaggta aaggttatga aattaccgat
    28741 gagtctcttt tgtcaggtct tggtgaccca ctaggtcaag cgacttacca gcttggttta
    28801 gctattgcca acaagatcga taatgatttg gtagcggtag ctaaaactgc aacacaacat
    28861 attacagaaa ctccaacaac tcttgaggca atcgataaag ctctagatat ctttgaggac
    28921 gaagaagatg cacagtatgt tgctatcatc aaccctaaag atgctactaa gctaaaaact
    28981 gcagtagcaa aagaatggat taaaggttca gagcttggag caaatatggt tgtttctgga
    29041 accttcggtg aagttgatgg tgtgcaaatc gtgcgctcta aaaaagttga tgaaggtaaa
    29101 ggcttccttg ttaaagtgtc accaagtcaa actcagacag acgacgctaa caaatatgga
    29161 gcttttgtta tcttgcttaa acgtgatgtg gctatcgaaa cagaccgcga tatcttgaag
    29221 aagactaccg taatcacagg tgatgaacac tacggcgttt acctttacga ccctacacga
    29281 gttgtaaaat tcggtggcgc gtaagaggtg acgatatgag tttattgcta cgacgtcatt
    29341 atattcaaga agagcaggtt aaccagtatt ctgatttaga gaataaaact ctagaagagt
    29401 tgaaggatct agcgaaagaa gcaggtgtag caggcgctta taagttgaca aaagccgaaa
    29461 ttgtagaagt tttggaggaa ctaaaaagtg aaatttaaaa tcaaacaaga tttctatgat
    29521 tgggaatcaa atgtgaaacg actggcagga gaggaacttg agattactga ggagcgctat
    29581 gctgagctgg ctgacaatat tgccagcaac ggtgtcacta tctcagacgt tcttgagaaa
    29641 atcctccctg aacctgagtt cttagaagag gattgatatg tctatagagt tgctgaagaa
    29701 attaacaggc gaagaagata ctcagcttct catgttgctc caaacgaggg ctacaaatct
    29761 tatcttgtca gagactaatc gcacatcttt gacacctgct ttaagtcttt tgatacctga
    29821 ggttgctatc gagctccaca accgctcagg agcggaagga gagcattcta gaaccgaggg
    29881 tggtatagca gtagtctacg gagaaaacgg cctatctacg ggtcttctac agcgaatacg
    29941 catgcacagg ctagcaaggg tggcaggtca tgtttttgaa gcagagtaga ctgaaaccct
    30001 atccaatgcg acggtttgaa aagactgtca ctgaggaagg tgtcgcgaaa gaagggtatg
    30061 ccaaggaagc tgagacagtc cgccttgaat tgtggccagc tagtagcaag ctacaatctg
    30121 aattgtatgg cgagcgtgtc aatgatattt tgaacgcaaa tgccaacaag tcagctacta
    30181 tcaaagtaaa agatggtgtg tgtatcgata gtcagacgga agtgactcat agggttattt
    30241 ctaaaaaggt ctacacacat catcaagttt tggagttaga gcgtgtcagg gctactaggg
    30301 gcagataggc ttatagctaa atgtagacgg ttagctagta aaaaaactgg cgaggatatc
    30361 gtcttacgtg cggtacacaa tgctgctata aaagttgtcc aagctgatgc aagaagactc
    30421 gtaccagtga atgatggaga acttataact agtatcaaaa ccagagcaaa aatggacgga
    30481 gatagggcta taggcgaagt ttacaccaac cttaaatacg ctccttacgt tgagtttgga
    30541 acgggaccta agggtcaggc tagccattct ggtatctctc cagaggtcag cgtgacttac
    30601 aagtctcatc cttggtatgt gcatgaagac caaatcaatg taggatctta ccactttcaa
    30661 aagattgggg agttttacaa gatgtatggt caacctgctc agccttatct ttatccagct
    30721 ttgagagaca atcaagagcg tgtgtctaag aatatttcga attatgtccg tagaaagata
    30781 agagaacaaa tacaatgatc aatatcaagc ctgttattta taaagaattg caaaaggttg
    30841 cagataatgt gaccgatact tatcctagcg attgggagac tttcccagtc gttatttttt
    30901 tagaagaaca aaacaagcca ggtgattggt ttgacgacaa ggaacaaaaa tcctctatcc
    30961 gctataaggt ggatattttt gatgatacca gcactagtga gttagctgtt aaaatcaatc
    31021 agatttttga gtctttaggt ttgcgaagaa ccgactgcca agacgtgcca gacccgtctc
    31081 atttgagaca taaggtcatg cgttttgaag gtgtcgttga cttagattca gagcttgttt
    31141 ttcaatttag aatggagaat taaaatatat gttagcaaat ggaattacgc tttcttatag
    31201 caaaacaaaa ggtagctata ctaagcttgt tggattgaaa gaagtgccag agtttggtat
    31261 tgaactcgaa aaagtagaaa atactactct tgaagatacg gtgaagaagt acgagcttgg
    31321 tattggggac gtaggagaac ttgagtacaa gttctcttat aataattcaa gcgcaactgc
    31381 tccttaccgt gtattgcgta aggcagcaga cgacaaggaa aaactctact tcgaacaagc
    31441 ttatccagac ggtactaagg tcacatttga aggtcaagta tccgtaaaac ttggcggtgg
    31501 cggtgtcaat gccgttatcg atttcaccct taaaattgct ttacagtcag agttggaatt
    31561 tgcagatggt gttggaggtt aattaaatgg cgttaaaata cacaacttgg aaagttactg
    31621 acgaaaaaga gttgaagcta cgtttgacat ctcatcaagc tgcaactgtg gaagaaaaaa
    31681 tcggcatgaa cttgctgaag attttcatgc ctgaagcagg cgaagagttc actttgccac
    31741 ctttgaaagt tatgttgttg ttagttcacg gcgccttgca gcagtatgaa catgggtatt
    31801 ctcttgagga tgtctatgat ttatacgatg aatacgtgga caatggtgga gaccaaacaa
    31861 cattcatgac agaggtgtta atgccactct ttgaagtatc gggttttact ccacgaggaa
    31921 gcaaggacaa gaaaacttcc aagaagaaaa tgacagtagt ccagtaatct taacggtaac
    31981 gcagattatt gagagacttt acccaatgtt tttggacatc ggaggtaagc ctcttgattt
    32041 ttgggatttg acggtgcttg aaatcaggga aatgatagaa agctacaacc gtgtcaaaat
    32101 ccaagagcgt aaagagaaga ttattgactc atacagactt tcgcagatga tatccaacca
    32161 cgtttctttg ttattgtcca aggatgccaa ggtttttgag ttctgggaat atgcgcctga
    32221 gttgtttgta gaagaacagc aagcagtaga acaggaacga cagagacaag cgcttttgtt
    32281 gcataaggaa cggatgcgtg attttgcaga gagacacaat cgcaaaagaa aggaggaagt
    32341 gaatggcaac tcttgatgaa ttgaaggtca tgattgacgc tgagatagcg cctttcagga
    32401 agaagatgaa agaagtcgag aatcaggtca aaggaacatc tgaccaagtg aaaaatgcca
    32461 ctgccaaagt tcgtgaacag tcgaactcta tcggtagtgc gtttggcaag ctggctaagt
    32521 tcgctggttt tgcaatcctt ggtaagaaat tacttgatgt tgggatgtat tcaacgcaga
    32581 cagctcttga agtgtcagcg tctatgaacc aaatcaagcg tcagatgggc gagagttcgc
    32641 aatctttctt aaaatgggtt aacgataacg caaatgcaat gaatatgggt gtaggtgaag
    32701 cggccaggta cggtgcggtt tactccaacc tgttttctgg atttatcaaa gataccaata
    32761 agctaagcgc ctatactgct aagatgttgc agacatcggc agtggttgct gaaggttcag
    32821 ggcgcacgat tacagacgtt atggagcgga ttcgctcagg tctactaggg aacaccgaag
    32881 caattgagga cctaggaatc aacgtcaatg tggctatgat taagtccact gaagctttta
    32941 aacgtttctc aaatggtcaa agttgggacc aactcgactt tcaaacccag caacaaatcc
    33001 gtcttatggc tatcctggag caagctacag ccaagtatgg agataccttg tctaattctg
    33061 taaatggtcg tatcagtctg tttaagtcgc taatgaagga cgcagcattg aaccttggta
    33121 actctatgtt accgattatc aatgccatta tgcctgtctt gaactctttt gctatggtct
    33181 taaagaacgt tactgctaaa ctcgctgagt ttatcgcttt gatgttcaac aagaaagcaa
    33241 cagtgaaaga tggtgttggt ggagcagttg gagacatggg taacgccatg aaggatgctg
    33301 caggcggagc aggagacctt gctgacgcag tagacgacgc tggagattca gcaggaggac
    33361 ttgctgacaa tcttggagac tccgccaaaa acgctaagaa ggccgctaaa gagttgctag
    33421 gtcttttggg atttgatgag attaacatct tgcaaaaacc aaaagatgac gacgcaggcg
    33481 ggtctggagg cggtggcaaa ggtggtaaag gaaagggagg cggtggcgga cctttcaaag
    33541 acatcttgcc agaagtcgag ttgaccgaca tggacaacaa attcaagagc atttttgatg
    33601 gtcttggaga taagctcaaa gggttgtttg acctcttcaa gaaaggtttt gatgcagcat
    33661 ttagaccaga aggtataaaa cgcattaaga ctgccttaga ccaaatagct aagacaatgg
    33721 gagaaatcgc cactgaccca agggttgtga atgcctttaa ccgaatggct gagaaaattg
    33781 cttatgcttt agggcaagtg acaggctcaa tagctactat cgggctaggt atcggtgttt
    33841 tccttgccga aagtattgcc aatggccttg gaaggcaaaa agaacgcatt atcaaggcgc
    33901 tagtcgcttt gtttgataat attggtaaca ttgccgaggc agtaggaaac atcgctcagg
    33961 acttttctag tgctttctac gacgtcatta cctcaactgg tgcggttcgt atcggtagcg
    34021 ctattgtgtc aactctgttg agtttgacat ctaccattgt tgaagttggt agtaaattag
    34081 caggaagttt gtttaaagat tttgaaaaag tcgttgtgac aaacgctcct aaaatttcat
    34141 caatcttcca aagtttatta gatactgttg cgcctgtatt tgagagcatt gaaaggtctg
    34201 ttaacaaatt tggcgatggc ttaagtcgtg tttatgatga acatgtagcc cctgctatta
    34261 actctattgc taatgctttt aatgggctaa ttgacattat tcaaatactt tgggaaggaa
    34321 gttggaagcc ttttgcagag ttcttgtcta acacattcgg cataagtatt gaaaccgtcg
    34381 ctgatttact aggcggtatc atactagagg cattgaagtt actagctgat acaatcaagc
    34441 tagtagccga tggttttact gctttttcag attggtgtaa agaaaataaa gagattatct
    34501 ccacaatcgc tagtgtgatt ggtacgcttg caaccgtgtg gcaaggaatt aagttcttgt
    34561 cttgggctga acaagctgga ggacttgcag gggcattcga attattaagt agtaaggttt
    34621 cctttattgt tagcggaatt aaaaatcttg gactagcttt gaaagctttg acatttgata
    34681 aattggtcag cttcggagaa accatctatt tgaatgcgtt gtatgcaaaa gattttgttg
    34741 tcaattcagg taaaacaatt gcacagctag ggaaaactgc tttagaactt ggtaaatcag
    34801 ctctagcatg gactgctcat acagcgaaaa tgggattagc aaccgcggcg gaatttgcac
    34861 attctgtggc agcaggagtc gctacagctg caacatgggc ttttaatgca gcgttagcag
    34921 ttttgacaag tccaataacc ttagttattg ctgctattgc agccttaatt gctattggtg
    34981 tcttgctcta ccaaaactgg gacactgttg ttgagtttgc taaaactgca tggcaaggac
    35041 tatgtgattt tattagtggt atttgtcgag ctattggcga atttttcagt ggtctatgga
    35101 caaaactaca agaaatcttt gagccaatag gtcaatggtt tggcgagaag ttccagcaag
    35161 catgggatgc tatcgttaat atcttcacac caatcggctc atggtttgga caacgttggg
    35221 cggatgtgac tagtgccttg gctaatatcg gggcatggtt tactgacatg ttccaaaaag
    35281 catggactgg tctaacaaac atctttagca aactaggttt atggtttggc gagagatggg
    35341 cagatgttac aagtgttctt gcgaatgtat cttcttggtt tgggaatatg tttactagtg
    35401 cttataatgc agtcaagaac gcgtttagtt caattggtgg cttcttcagc ggtgtatggt
    35461 caacggttca aagcatattt gtcaatgctg gacaaaaggt tggtagcgct gtaggtgggg
    35521 ctttcagaag tgcagtcaat ggtgttcttg ggactattga aaatgtagtc aatggcttca
    35581 tcggcatgat caatggtgtt attggcatga ttaacaaaat acctggcgta tcccttggtg
    35641 gtattggata tgtgagccta cctcgtcttg cccgtggtgg tatcgtcgat agcccaacaa
    35701 tcgccatgat tggtgaagct ggtaaagaag cggtcgtacc acttgaaaat acaggcttta
    35761 tccaaaccct tgggcgagtt gtcagcagtg cggtagtaaa tgccatggct ggtattagtc
    35821 cacaaggtgg tttttctggt gacggcgaca tcgttattca aatcgcaggc catgagttcg
    35881 gacgggtagc tatccaagaa atcaacaagg aacatgaacg agcaggtcaa accttgctca
    35941 agatttagga ggttaaatgg cacaattgac aatcaatggg gtggctgtga agcctcccaa
    36001 atcttttaaa gtcggtattc aagatatcga tggagagaca gggcgtaatg ccaatggcga
    36061 catggtgcgt gaccgtatca cgaccaaacg caaactagac tgtgaatggg gtatgatgac
    36121 tcagggagaa ataagtcagc ttttacatgc tgtatcatct gaattttttg aggtgtctta
    36181 tccagacccc atggatggcc aagtcacaaa gactttctat gtcggtgcta ggacagctcc
    36241 tagctatacc tttactgaga agtttaaacc ttggtctggc gctaaattta atctggtaga
    36301 gaggtaagaa aatggacgct ttaactagac gacaatttga cagagccatg tttgccaaaa
    36361 acaggacgct ggctatccgt gttggagatt atgcttcaca ggatatcaaa gaggctagct
    36421 ttgagtatgg ctacattaag ggcgatactt ataagcctgg tggaacctgc gctggtagcg
    36481 gtaaaattac ctttaccagc atcattacca cgttcaataa gctggatacc ctgcaccctg
    36541 agattggtct actggttggg gatacctacc agtgggtcaa gatgggggaa tacttcatca
    36601 acgatattga gattgaccga aaccgaaaca ctaccacgct tgaacttatg gacggtatgt
    36661 ttaagctcaa tcgtgagtac gtgacggact tgcatttccc agctgaagta cgagaggtta
    36721 ttcaggaaat ctgcctgaaa acaggcattg agttagcgaa tgactatttc ggaatcagcg
    36781 ctatgcgtta ccatatcgag caagttcttg aaggtaagaa actttcgttc agggatatgc
    36841 tgagcgctat gactcagatg attgggatgt cttgtttctt caacagagaa ggcaagatgg
    36901 aaatccgtga tttgactgag tccaatatca cgatcaacgc tgacagttac ttcttgcatg
    36961 gcttgaccaa gagtgagatt gagtatcaga tatctggtat cacttgtaag acggacaaga
    37021 agtctctgac ggtcggtatg aagacaggtc ggtctttgga actggacaat gtcttcatga
    37081 cccagagtgc tttaaatgac ctgtattaca aactgaaaaa cctaacttac tatccgtata
    37141 atctcaacta ccaagggcat ttactgcttg aggttgggca gtgggtaacc attcagacca
    37201 ataagaaaga gacttttaaa gttcctgtct taagtcagag ctttactttc aaaggtggtc
    37261 tgagaggtcg tatcagcgca gatagtaaag ctggaaatga tactcagtat tcttacgagg
    37321 gaacgattac caagcagata aagcaacaag atggcgttga agcaaaagtc caagcgcaga
    37381 ttgaagcagc agataaagat tttgaccaaa aggtcgacaa aatcaaaaaa gactttaacg
    37441 atcaagtaga actggccaaa gccagagctg aagaagtcaa gagagaactg tctgacacta
    37501 tcaatcagcg ctttaatagc tttgacaacg ggccattgaa agaagctaag cgcaaggctg
    37561 aggaagcttt gcgaaatgct ggcgcaagta ccctgcttgc acaggaagct aagcggattg
    37621 ggctggattc tgtcgctaga cttgaagcgt ttaagtcgca gactacgagc gcacaaacgg
    37681 ctctgtcggg tgacttggac gctctgaaac ggactatcgt gaatgatatt cgaccgaagc
    37741 aagcacaggc tgaaactgag attgccaagc aagttgaagc acttagccgg actaagaatg
    37801 aactggctgg cgcaagtacc ctgtttgcac aggaagctaa gcggattgag ctggattctg
    37861 tcgctagact tgaagcgttt aagtcgcaga ctacgagcgc acaaacggct ctgtcaggtg
    37921 acttggatgt tctaaaacga actatcgcaa acgatattcg accgaagcaa gcacaggctg
    37981 aagctgagat tgccaagcaa gttgaagcac ttagccggac taaaaatgaa ctggctggcg
    38041 tgaagtcagc gcaagcgacg tatgaggaga cgacgactcg tagactgtca gaactgacca
    38101 acttggctaa tggtaaagcc agcaagtcag aactcacgca gacagctgag gagctctcta
    38161 gtaagatagc gagtgtgcag gcatccggtc gaaatctatt cttgaactca ctattcaagc
    38221 aggatatttc aaaaacagga atttggacaa cgagtacata tacggctgct atcgatagcg
    38281 aaagtaagta tcttggacac aaggctctta aaattatagg tttgaatcca tctggtcgtg
    38341 atggaggtaa tcccaaggtt acttatccag ctctgggtca attcgggaaa gtaattcccg
    38401 gaagtacgac taatcaagat gtaaccatta gtttttatgc taaggcaaat aaaaatggaa
    38461 taatgctaag atctcgatta gggaatatcg gatataaaac tggaaatgtg acattgtcga
    38521 cagaaattaa gcgatatgtt gtccatattc caaaaggttg gacaaacgaa tccaagcaga
    38581 ccacaaatga atggttgttc aatttcaacc aggaaggaac catttggatt tggatgccga
    38641 agtttgaaat aagcgatgta gatacttctt attcagaagc tcctgaagat atagaaggtc
    38701 agatttcaac agttgaatcc aactttaaac agcgtgctga ttcactcgaa gctggtgtaa
    38761 gccgtctgac tgaaggcctt agaaccaaag cggatatcag cgcactcaat gtgactgctg
    38821 aaaatatccg gcaatctgtg aagagtcttg agacagacac gcagaacaag ctaaatcaga
    38881 agttgagtca ggctgaattt gaggtgcgag ccggctctat ccgtcaggaa atcctgaacg
    38941 caaccaagga caaggcagat aagactctag ttgtatctga agccgggaaa ttgcgtgaag
    39001 aattttcaaa aatgaaggtg ggaggccgga atctatggat aaaatccaag acggttggag
    39061 ctgtaattga aaaattacct gaaaacca
//
LOCUS       DAVLRT010000012        36239 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLRT010000012 DAVLRT010000000
VERSION     DAVLRT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.012"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            361..1054
                     /locus_tag="VXM85_000601"
                     /pseudo
     CDS             361..1054
                     /locus_tag="VXM85_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            1108..1497
                     /locus_tag="VXM85_000602"
     CDS             1108..1497
                     /locus_tag="VXM85_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1345227.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(2158..3186)
                     /gene="queA"
                     /locus_tag="VXM85_000603"
     CDS             complement(2158..3186)
                     /gene="queA"
                     /locus_tag="VXM85_000603"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1345228.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            3337..4044
                     /locus_tag="VXM85_000604"
     CDS             3337..4044
                     /locus_tag="VXM85_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1345229.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            4122..4631
                     /locus_tag="VXM85_000605"
     CDS             4122..4631
                     /locus_tag="VXM85_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345230.1"
                     /translation="MNKEEFLKIKEAYKSARTEERKSIIGFITKKKDKEGNFLFTKSK
                     DKPYTTRNQYSGGGGNKKYTSGSRLSRPYDLSNHMWIDLSYKGNDILISLQSFDIDPN
                     SKELHVLYDRIGILFEQSKKIPIFKDCYTITKVSDAFLKMETTNWELPLSKADMEEMV
                     NYIINHYEE"
     gene            4711..4887
                     /locus_tag="VXM85_000606"
     CDS             4711..4887
                     /locus_tag="VXM85_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1345231.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            5017..5952
                     /locus_tag="VXM85_000607"
     CDS             5017..5952
                     /locus_tag="VXM85_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1345232.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINIADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            5945..6733
                     /locus_tag="VXM85_000608"
     CDS             5945..6733
                     /locus_tag="VXM85_000608"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1345233.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            6734..7117
                     /locus_tag="VXM85_000609"
     CDS             6734..7117
                     /locus_tag="VXM85_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1345234.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            7133..7522
                     /locus_tag="VXM85_000610"
     CDS             7133..7522
                     /locus_tag="VXM85_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1345235.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIHITEE"
     gene            7606..8736
                     /locus_tag="VXM85_000611"
     CDS             7606..8736
                     /locus_tag="VXM85_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1345236.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            8741..9478
                     /locus_tag="VXM85_000612"
     CDS             8741..9478
                     /locus_tag="VXM85_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1345237.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            9489..10262
                     /locus_tag="VXM85_000613"
     CDS             9489..10262
                     /locus_tag="VXM85_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1345238.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            10252..10869
                     /locus_tag="VXM85_000614"
     CDS             10252..10869
                     /locus_tag="VXM85_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1345239.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            11079..11480
                     /locus_tag="VXM85_000615"
     CDS             11079..11480
                     /locus_tag="VXM85_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1345240.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            11480..11758
                     /locus_tag="VXM85_000616"
     CDS             11480..11758
                     /locus_tag="VXM85_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1345241.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            11748..12521
                     /locus_tag="VXM85_000617"
     CDS             11748..12521
                     /locus_tag="VXM85_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1345242.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSSTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            12522..13826
                     /locus_tag="VXM85_000618"
     CDS             12522..13826
                     /locus_tag="VXM85_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1345243.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            14400..15278
                     /gene="pstS"
                     /locus_tag="VXM85_000619"
     CDS             14400..15278
                     /gene="pstS"
                     /locus_tag="VXM85_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1345244.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            15278..16195
                     /gene="pstC"
                     /locus_tag="VXM85_000620"
     CDS             15278..16195
                     /gene="pstC"
                     /locus_tag="VXM85_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1345245.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            16185..17069
                     /gene="pstA"
                     /locus_tag="VXM85_000621"
     CDS             16185..17069
                     /gene="pstA"
                     /locus_tag="VXM85_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1345246.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            17080..17883
                     /gene="pstB"
                     /locus_tag="VXM85_000622"
     CDS             17080..17883
                     /gene="pstB"
                     /locus_tag="VXM85_000622"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1345247.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKDFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            17896..18654
                     /gene="pstB"
                     /locus_tag="VXM85_000623"
     CDS             17896..18654
                     /gene="pstB"
                     /locus_tag="VXM85_000623"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1345248.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            18666..19319
                     /gene="phoU"
                     /locus_tag="VXM85_000624"
     CDS             18666..19319
                     /gene="phoU"
                     /locus_tag="VXM85_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1345249.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            19476..20291
                     /locus_tag="VXM85_000625"
     CDS             19476..20291
                     /locus_tag="VXM85_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1345250.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKNEGTYQSYLEKAASLTEVEE"
     gene            complement(20311..21582)
                     /locus_tag="VXM85_000626"
     CDS             complement(20311..21582)
                     /locus_tag="VXM85_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1345251.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            21724..22455
                     /gene="budA"
                     /locus_tag="VXM85_000627"
     CDS             21724..22455
                     /gene="budA"
                     /locus_tag="VXM85_000627"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1345252.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            22465..23217
                     /locus_tag="VXM85_000628"
     CDS             22465..23217
                     /locus_tag="VXM85_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1345253.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            23329..24279
                     /gene="murB"
                     /locus_tag="VXM85_000629"
     CDS             23329..24279
                     /gene="murB"
                     /locus_tag="VXM85_000629"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1345254.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            24338..25495
                     /locus_tag="VXM85_000630"
     CDS             24338..25495
                     /locus_tag="VXM85_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345255.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            25476..26282
                     /locus_tag="VXM85_000631"
     CDS             25476..26282
                     /locus_tag="VXM85_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345256.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            26279..27052
                     /locus_tag="VXM85_000632"
     CDS             26279..27052
                     /locus_tag="VXM85_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345257.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            27049..28119
                     /locus_tag="VXM85_000633"
     CDS             27049..28119
                     /locus_tag="VXM85_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1345258.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYEAFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            28380..28619
                     /locus_tag="VXM85_000634"
     CDS             28380..28619
                     /locus_tag="VXM85_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345259.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLNDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            28894..29379
                     /locus_tag="VXM85_000635"
     CDS             28894..29379
                     /locus_tag="VXM85_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1345260.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            29401..32019
                     /gene="alaS"
                     /locus_tag="VXM85_000636"
     CDS             29401..32019
                     /gene="alaS"
                     /locus_tag="VXM85_000636"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1345261.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(32109..33563)
                     /locus_tag="VXM85_000637"
     CDS             complement(32109..33563)
                     /locus_tag="VXM85_000637"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1345262.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANKELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            33742..34491
                     /locus_tag="VXM85_000638"
     CDS             33742..34491
                     /locus_tag="VXM85_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345263.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            34472..36106
                     /locus_tag="VXM85_000639"
     CDS             34472..36106
                     /locus_tag="VXM85_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345264.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSLTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLPILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
BASE COUNT        10369 a         6824 c         8234 g        10812 t
ORIGIN      
        1 tgagaaaact attctattta tactgcatag ttgtataagg aatgatgaaa gatgagtgaa
       61 agttatcaag ttggaatgtt tgtatctaaa tatattagca tgtatttaga taagatgtct
      121 gcaatccttt atatatgaga ttgattacga tatagttaaa gtgttagttc aataatcaat
      181 atatcattct aagtctattt tatatataaa gtatattgtt taacgtttga tagaaaagat
      241 gctatactag aagtaggatg taatggcaaa atctaatact aataagcgct atgcaatacg
      301 taaactcgga aagatttttg gttctgcggt aatcgggcta actttatttt taggaggaat
      361 ctgaatacaa gttttgttca tgctgctgat gggattcaat atgtcagaga tgatactaga
      421 gataaagaag agggaataga gtatgatgac gctgacaatg gggatattat tgtaaaagta
      481 gcgactaaac ctaaggtagt aaccaagaaa atttcaagta cgcgaattcg ttatgaaaaa
      541 gatgaaacaa aagaccgtag tgaaaatcct gttacaattg atggagagga tggctatgta
      601 actacgacaa ggacctacga tgttaatcca gagactggtt atgttaccga acaggttact
      661 gttgatagaa aagaagccac ggatacagtt atcaaagttc cagctaaaag caaggttgaa
      721 gaagttcttg ttccatttgc tactaaatat gaagcagaca atgacctttc tgcaggacag
      781 gagcaagaga ttactctagg aaagaatggg aaaacagtta caacgataac ttataatgta
      841 gatggaaaga gtggacaagt aactgagagt actttaagtc aaaaaaaaag actctcaaac
      901 aagagttgtt aaaaaaggaa ccaagcccca agttcttgtc caagaaattc caatcgaaac
      961 aaaatatctc gatggcccaa ctcttgataa aagtcaagaa gtagaagaag taggagaaat
     1021 tggtaaatta ctcttactac aatctatact gtagatgaac gtgatggaac aattgaagaa
     1081 actacttctc gtcaaattac taaagagatg gtaaaaagac gtataaggag agggacgaga
     1141 gaacctgaaa aagttgttgt tcctgagcaa tcatctattc cttcgtatcc tgtatctgtt
     1201 acatctaacc aaggaacaga tgtagcagta gaaccagcta aagcagttgc tccaacaaca
     1261 gactggaaac aagaaaatgg tatgtggtat ttttataata ctgatggttc catggcaaca
     1321 ggttgggtac aagttaatgg ttcatggtac tacctcaaca gcaacggttc tatgaaagtc
     1381 aatcaatggt tccaagttgg tggtaaatgg tattatgtaa atgcatcggg tgagttagcg
     1441 gtcaatacaa gtatagatgg ctatagagtc aatgataatg gtgaatgggt gcgttaacag
     1501 attcctccct gcgacaaaat tgataatagt atgtctgaat atataattat atataaaaag
     1561 ctatagataa ggtcatctta tagcgactat aaaaaagtga acaagcctag tcttctgtac
     1621 attagaattc agatttgttc actttttgtt taatactcaa tgaaaatcaa agagcaaact
     1681 agaaaactag tcgcaggttg ctcaaagtac tgctttgagg ttgtagatag aactgacgaa
     1741 gtcagtcaca tatataatcc aaggtgacgt tgacgcggtt tgaagagatt tttgaagagt
     1801 ataagtcttt gattttgaga ctaaggagag tcagggtcaa agtagtgaat gattctgaaa
     1861 taactcataa tagtctcttg agaggacaga taagagtaac aaatatggtg ctactcatgt
     1921 gtcttgcatt ttgatttaca aacagcgaag aaaatatagg aagctgaaat aaaatataga
     1981 tgaatcaatt tggagagtca acgcaatcta tggcaatatt ttcgaagcta gggggaagca
     2041 ttttaatttc aatgtactag gcctcttcta cagctagcta gagtttcaat tgtagatgtt
     2101 ctttgaaata atatcttagc gatttattgg tattagaaga tttagagaaa ttctttctca
     2161 ataaataaac atggcgtcac caaaactgaa gaagcggtag tgttcttgga tggcatggtg
     2221 gtaggcatct aagactaatt cacggcctgc aaaggcagaa accaacatga ccagagttga
     2281 ttttggcagg tggaagttgg ttgagaaggc atccacgacc ttccactcat acccaggttt
     2341 gataaagata ttggtccaac cagaatctgc ttggatttgc ccatcaaact tggaaccaat
     2401 agtttccaag gtgcggatag aagtggttcc gacagcgatg acacgaccac cattttcttt
     2461 gacagagcga agggtggcag cagcttcctc agaaagttga tagaactctg agtgcatttc
     2521 gtgttcgtcc agattatcca cagaaacagg tctaaaggtt ccgagtccga catggagagt
     2581 cagatagact agatgaacac ccttagcttg gatttctgcc agcagttctt tggtgaagtg
     2641 aagaccagca gtcggtgctg cagcagagcc gctttccttg gcgtagacgg tttgataacg
     2701 ttcacggtca tctaattttt cgtggatata aggtggcaga ggcatttctc ccagactttc
     2761 caagacttct aggaaaattc cttggtattc aaagcggaca atgcgtcccc cgtgggtcaa
     2821 ttcttctgta acgacagcgc tgaggcggcc atcaccaaag ctgatacgag taccgacctt
     2881 gaggcgtttg gcaggtttag ccagaacttc ccactcgtct ccactagtgt tcttaaggag
     2941 gagaagttcc acatgacctc ctgtctccac tttttgacca tagaggcggg cagggagaac
     3001 tcgggtgtcg ttcatgacaa gggcatcacc aggttccagc atatcaataa tagagtggaa
     3061 gtgtttatct tgcatttctc ctgtctcacg gttgacgatg aggagtttgg aggcatcacg
     3121 tttttcaagg ggcgtttggg caatcaattc ctctggcaag tggaaatcaa aatcagctgt
     3181 attcatatat ttgttcattc tttctaagtt ctaatcctat ccattataac acgtttcaag
     3241 tttggacgct ctttttttag aaattaaatc gttttcactt gacaaaaatt ggtctatacc
     3301 atataataaa tatagacaga aatggaggat gaaaagatga aagttattaa agttgaaaac
     3361 caagttcaag gtggaaaagt tgcttttgag attttgaagg aaaaattggc taatggagct
     3421 caaactctag gacttgcgac aggaagtagc ccacttgaat tttacaagga aattgttgag
     3481 agtgaccttg atttttcaaa tctaaccagt gtcaaccttg atgagtatgt gggccttgat
     3541 ggggacaatc cacagtctta tcgttacttc atgcaagaaa acttgttcaa ccaaaaacca
     3601 tttaaagaaa gtttcttgcc tcgtggtgtt aaggacaatg ctgaagctga agttgaacgc
     3661 tacaaccaaa ttttggctga ccatccagtt gacctccaaa tcttgggaat cggtcgcaat
     3721 gggcatatcg gctttaatga gcctggtact ccatttgaca gtcaaacgca tctagtagaa
     3781 cttgaccagt ctactatcga agccaatgca cgcttctttg ccaagattga agacgtccca
     3841 acccaagcta tttcaatggg gattaaaaac attttggatg ccaagtctat tattcttttt
     3901 gcttacggtg agtcgaaagc agaagccatt gccggaacag tatctggccc agtgactgag
     3961 aacctacctg caagtagcct ccaaaatcac cctgatgtaa ccatcatcgc agatgctgaa
     4021 gcgcttagct tactcgaaaa ataaaaagta taagaaccgc ttgctctttg gagtgagtgg
     4081 ttttagacat ataaaacttc aacttaactt taggaggaca catgaataaa gaagaatttt
     4141 taaaaataaa agaagcatat aagagtgcta gaacggaaga aagaaaaagt ataataggct
     4201 tcattacaaa aaagaaagat aaggagggga attttctttt tactaagtca aaagataaac
     4261 catataccac aagaaatcaa tattcaggag gcggaggaaa taaaaagtac acctcgggta
     4321 gtagattatc taggccatat gatttatcaa atcacatgtg gattgatttg tcatataaag
     4381 gaaatgacat cttaatctct ttacagtctt ttgatattga tccaaatagt aaagaacttc
     4441 acgtcttata tgatagaata ggcatcttat ttgaacaatc taaaaaaatt ccaattttta
     4501 aagattgcta tacaataact aaagtcagtg atgcttttct taaaatggaa acaacaaact
     4561 gggaattacc tttatctaaa gcagatatgg aagagatggt gaactatatt attaatcatt
     4621 atgaagaata aaaaacttag tttcctcttg acaattattc cttttacgtg tagaatgaaa
     4681 tagatattga acttgaaggg agtgaaaaaa atgtctaaaa cagtagtacg taagaatgaa
     4741 tctcttgacg acgcacttcg ccgtttcaaa cgtgcggtta ctaaagctgg tactcttcaa
     4801 gaaacacgca aacgtgaatt ctatgaaaaa ccttctgtaa aacgtaaacg taaatcagaa
     4861 gtagctcgta aacgtaaaaa attctaattg aaatgaaagg ctagagaaat ctagtccttt
     4921 ttcttttaaa taaatactcc aaagcctgca aaaatctgaa acttcctcct acaatttgat
     4981 ataatagaga gaagaactca tttgaaggag gaaatgatgt cggttttagt aaaagaagtg
     5041 attgaaaagc ttagactaga cattgtctat ggtgaaccag aattgcttga aaaggaaatc
     5101 aatatagcgg atattacgcg acctggtctt gaaatgacag gctattttga ctactataca
     5161 ccagagcgga ttcaactttt ggggatgaag gagtggtctt atctgatcag catgccttcc
     5221 aacagccgtt atgaagtttt gaaaaaaatg tttctacctg agacaccagc agtcattgtt
     5281 gcccgtggtt tggtggttcc agaggagatg ttaaaggctg ctagagaatg taagattgct
     5341 attttaacca gccgtgcagc taccagtcgt ttatctggag agttatctag ctatctggat
     5401 tctcgtttgg cagaacgtac cagtgtgcac ggtgtcttga tggatattta tgggatgggc
     5461 gtcttgattc agggagatag tggaattggt aagagtgaga caggtcttga gcttgtcaaa
     5521 cgtggtcacc gtttggtagc cgatgaccgt gtcgatatct ttgccaagga tgagattact
     5581 ctctggggtg aaccagctga aattttgaaa cacttgattg aaattcgtgg ggttggtatt
     5641 atcgatgtta tgagtctcta cggtgcgagt gctgtcaagg attcttcaca ggttcagctt
     5701 gctgtctatt tggaaaatta cgatacgcat aagacctttg atcgtcttgg aaacaatgca
     5761 gaggaacttg aagtttctgg cgtagccatt cctcgtattc gtattccagt taaaacaggt
     5821 cgtaatatct ctgttgtgat tgaggcagct gccatgaatt atcgtgccaa ggaaatgggc
     5881 tttgatgcga cccgtttgtt cgacgaacga ctgacaagtc tcatcgctcg aaatgaggtg
     5941 caaaatgctt gatccaattg ctattcaact aggaccccta gccattcgtt ggtatgcctt
     6001 atgtattgtg acaggcttga ttcttgcggt ttatttgacc atgaaagaag cacctagaaa
     6061 gaagatcata ccagacgata ttttagattt tatcttagta gcctttccct tggctatttt
     6121 aggagctcgt ctctactatg ttattttccg atttgattac tatagtcaga atttaggaga
     6181 gatttttgcc atttggaatg gtggtttggc catttacggt ggtttgataa ctggggctct
     6241 tgtgctctat atctttgctg accgtaaact catcaatact tgggattttc tagatattgc
     6301 ggcgcctagc gttatgattg ctcaaagttt ggggcgttgg ggtaatttct ttaaccaaga
     6361 agcttatggt gcaacagtgg ataatctgga ttatctacct ggctttatcc gtgaccagat
     6421 gtatattgag gggagctacc gtcaaccgac tttcctttat gagtctctat ggaatctgct
     6481 tggctttgcc ttgattctga tttttagacg gaaatggaag agtctcagac gaggtcatat
     6541 cacggccttt tacttgattt ggtatggttt cggtcgtatg gtcatcgaag gtatgcgaac
     6601 agatagtctc atgttcttcg gacttcgagt gtcccaatgg ctgtcagttg tccttatcgg
     6661 tctcggtata atgatcgtta tttatcaaaa tcgaaagaag gccccttact atattacaga
     6721 ggaggaaaac taaatgttag aagttgcata tattcttgtt gccttagctt tgattgtctt
     6781 tttggtctat ctgatcatta ctgtacaaaa gcttggtcgt gtcatcgatg aaacagaaaa
     6841 gacgattaaa accttgactt cagatgtgga tgtgaccttg catcacacca atgagttgtt
     6901 ggctaaggtc aatgtcttgg cagatgatat caatgtcaag gtggctacga ttgatccact
     6961 cttcagtgct gttgcagatt tatctctatc tgtttcagac ctcaatgacc atgcgcgtgt
     7021 cttgagcaag aaagcttcat cagctggttc aaaaacactc aagactggtg caagtctgtc
     7081 agctcttcgt cttgcaagta aatttttcaa aaaataaaaa aggagaatcc ttatgggtaa
     7141 attatcctca atccttttag gaacggtttc aggtgcagct cttgccttgt ttttaacaag
     7201 tgataagggc aaacaagttt gcagtcaggc tcaagatttt ctagatgatt tgagagaaga
     7261 tccggagtat gccaaggagc aagtctgtga aaaactgaca gaagttaagg agcaggctac
     7321 agattttgtt ctgaaaacaa aagaacaggt tgagtcaggt gaaatcactg tggacagtat
     7381 acttgctcaa gctaaatcct atgcttttca agcgacagaa gcatcaaaaa atcaattaaa
     7441 taatctcaag gaacaatggc aagaaaaagc cgaagctctt gatgactcag aagagattgt
     7501 gattcatata acagaagaat aaaccatcac catctccgga cggactatgt atctggggat
     7561 ggtgattttt atctggaatc tagtctttgt ggtataataa ttactatgca gaaaaaacca
     7621 acgtcagcct atgtgcacat cccattttgt acccagattt gttattattg tgatttttca
     7681 aaggtcttca tcaaaaatca gccagtcgac agctatttag agcatctgct ggaagagttt
     7741 cgttcttatg atattgaaaa gttgtcaacc ctttatatcg gtggtggaac accgacagcc
     7801 ctgtcggctc cgcaactgga ggtgttactg aatggcttga ctaaaaactt ggatttgtct
     7861 gtcttggaag agttgaccat tgaagccaat ccaggcgatt tggatgcgga taagatagct
     7921 gttttgaaaa attcggctgt caatcgtgtt tcgctaggtg tccagacctt tgatgataag
     7981 atgttgaaaa agattgggcg cagtcatttg gagaaggata tttatgaaaa tatcgatcgc
     8041 ctgaaactgg ctggttttga caacatctcc attgatttga tttatgctct gcctggtcag
     8101 accatggagc aagtaaagga aaatgtggct aaagccattg gattggatat tccccacatg
     8161 agtttgtata gtttgatttt agaaaaccat acggtcttta tgaaccggat gcgacgtggg
     8221 aaattgcctc tgcctaagga ggaactagaa gcggagatgt ttgagtacat cattgcagag
     8281 ctggagcgag cgggttttga gcattatgag atttctaatt tctccaaacc cggttttgaa
     8341 agtcgtcata atctcatgta ctgggacaat gctgaatact atggtattgg tgcaggggca
     8401 tctggttatg tcaatggagt acgctataaa aatcatggtc ccattcgtca ttatctcagt
     8461 gcggttgagg aaggcaatgc ttgtattaca gaagatcacc tgagtcaaaa ggagcaaatg
     8521 gaagaagaaa tgttcttggg actccgcaag aaatccgggg tttccatggc gcgatttgag
     8581 gaaaaatttg gacagtcttt tgctggactt tatggagaaa ttgtcagaga tttggttcaa
     8641 caaggcctca tgcagattga gggtgaccac gttcgcatga caaagagagg tctctttttg
     8701 ggagatactg tagcagaacg atttattttg gagtaggatc atgggcttaa cttatcaaat
     8761 gaaaatgaaa attccttttg atatggctga tatgaacggt catatcaaac ttccagatgt
     8821 gattttgctg tcactgcaag tttcagggat gcaatcgatt gaactgggag ttagtgataa
     8881 ggccattttg gaagactata atctggtctg gattatcgca gaatatgata ttgaggtggt
     8941 tcgtttgcct cgttttgcgg aagaaattac catcgaaacg gaagccttga gctacaatcg
     9001 acttttttgc taccgtcgct ttactattta taatgaagcg gggcaggagc tcatccatat
     9061 gatggcgacc tttgttctca tggaccgcga cagtcgaaaa gtccatgctg ttgaacctga
     9121 gattgtggct ccttaccagt ctgattttga taaaaaactt atccgtggac cgaagtatga
     9181 atccttggaa gagccgatca gcaaggatta ccatgttcgt ttttacgact tggatatgaa
     9241 tggtcatgtc aataacagta aatacttgga ctggattttt gaggtcatgg gagcggattt
     9301 tttgacccac tatattccca agaaaatcaa cctcaagtat gtcaaggaag ttcgaccagg
     9361 tggggtgatt acatcggctg ttgaacggac tggactggaa agcaagcatg aaattacaag
     9421 tgatggggtt atcaatgccc aagcaatcat tacttggcaa gaaataaaaa aggtttaggg
     9481 agaaatagat gaaatataaa ggctatttaa tcgatttaga cggaaccatt tataagggga
     9541 aagaccgaat ccctgctgga gagacttttg ttcatgaatt gcaaaagcgg gacattccct
     9601 atctctttgt aaccaacaat acaacccgca ctccagagag tgttaaggag atgttggctc
     9661 agaattttaa tatcgatacg cctctatcga ctgtctacac agcgactttg gcaaccatcg
     9721 actatatgaa tgatttgggg cttgaaaaga ccgtctatgt cgtcggagaa gcaggactca
     9781 aggaagccat caaggcggct ggttatgtgg aagacaagga aaaccctgcc tacgtggtag
     9841 ttggtctgga ttggcaagtc gactatgaaa aatttgccac agcaactctt gctattcaaa
     9901 agggtgccca ttttatcgga accaatcctg acctcaacat cccgacggaa cgtggtcttt
     9961 tgccaggtgc tggctcactg attacgctgc ttgaagtagc gacacgagtg aagccggttt
    10021 atatcggaaa accaaatgcc atcattatgg acaaggcggt tgagcactta ggtttggaac
    10081 gtgaagagtt aatcatggtt ggggacaatt atttgactga tatccgggct gggattgaca
    10141 atggcattcc aacactcttg gtgacgacag gttttaccaa ggcagaagaa gtagctggcc
    10201 tgccaatcgc accgacacat gtggtttcta gccttgcgga gtgggatttt gatgaaaact
    10261 aaattgacct tttggggctc tatgctcttt ctcctctccc tctcaatcct tctgaccatt
    10321 tatctggctt ggattttcta tcctatggag attcagtggc taaacttaac gaatcgagtc
    10381 tatctaaaac cagaaaccat tcaatacaat tttcatatct tgatgaatta tctgaccaat
    10441 ccttttagtc aggtcttaca gatgcctgat tttcgttcgt cagcagctgg tctgcaccat
    10501 ttcgcagtgg tcaagaatct ctttcatttg gttcagctag tagctctagt gacactgcca
    10561 agtttctatg tctttgtcaa taggattgtg aaaaaggact ttttgtctct ttatcgaaaa
    10621 agtctcctga ctctagtagt cttacctgtg atgattggac ttgggggagt tttgattggt
    10681 tttgaccaat tctttactct tttccatcaa attctctttg tgggagatga tacctggctt
    10741 tttgatccag ccaaggatcc tgttattatg attttgccag agaccttctt tcttcatgcc
    10801 ttcctcctct tttttgccct ctatgaaagc ttctttgggt atctgtgtct gaaaagtcgt
    10861 aggaagtgaa atgaaagatg tttttaattt gcatatataa aaatatatat cgtttaaaac
    10921 catcgttaag agtgaattaa ccaaatccaa ttgtgtcaat cgttcgcaaa cagcctattt
    10981 ttcttgaaaa aataggcttt ttttctaaaa atccctagtc aagcgcctta tttttttgta
    11041 taatagaagt agcaaagtat agataagaag agaaaagcat gattacacta tttctatcac
    11101 cgagctgtac atcatgtcgt aaggctaagg cctggttaga aaaacataag gttccctttg
    11161 tggaacacaa tattatgacc agtcctttaa caagaaaaga attgcaacac atcctttcct
    11221 tgaccgaaaa tggtactgat gacatcattt caactcgttc aaaaattttt caaaaattga
    11281 atattgatgt agagagtatt tcggtatcgg aattgcttca tttgattgag cagtatccta
    11341 gtcttttgcg tcgtccaatt attattgatg ccaagcgtat gcaaatcggt tttaatgaag
    11401 atgagattcg tgcttttctc cctcgtagtt atcgtaagca agaactaaaa gaagcaagaa
    11461 tgagagctgg tattagttaa tgaacaaaca gtatagttac ccactagatt tgtcgtggag
    11521 cactgaagaa cttgcttcag tgctttcttt ttttaatgat gttgaagctg cctatgaagg
    11581 caaagtagag gctaaaaagt tactggactc ctataaggga ttcaaagcgg tcgtgccaag
    11641 caagagcgaa gagaaacgtt tgggacgaga atttgaaacg gttagtggct attcacttta
    11701 ccgagcagtt caggctgcca aagaaaaagg ggaaggaaag atttctcttg gaaagtaaat
    11761 ttgaatttgc taaagagctt gttaagaaag caggccagta cattcttgac catatgcagg
    11821 aagacttgcg tgttgaaacc aagtcctctt caacagattt ggtgaccaga ctggacaaag
    11881 aagttcagga actcttggtt ggtgagattt tgtcccgtta tcctgaggat aagatttgtg
    11941 ctgaagaagg ttgtctgcga gcctcggttc aagagggcaa ggtttgggtc attgatccca
    12001 ttgatggtac caataatttt gtagcccagc aggaagattt tgctgttatg atggcttatt
    12061 ttgaaaatgg tcagggacag tttggtctga tttatgatgt ggtcaaaggg gattgttacc
    12121 acggtggtgg ggaatttcca gtttgtctaa atgataggcc cctagctcct tttaaaacta
    12181 aaccacttgg agattttctc attgcaggga atagtggtat gctggaaacc aatgaatggg
    12241 ggctggctga tttgagccga tcggttcttg gtgttcgtgt ctatggaagt gcggccatta
    12301 gttttgccaa gattttgtca gggcgtttgt tgacctatct cacttatctg caaccatggg
    12361 attatgccgc agctagtatt ttaggggaaa gtctgggcta tcgggttgtg accctttttg
    12421 gtgaagctcc tgattttcaa accagacagc cggtcatgat ggtgcctctt gagatgcagg
    12481 aggaaattca gtcctatatt tacgaaagga aaagaactta aatgcaattt ccagaaggat
    12541 ttgttgaaaa atataaagag atactaggag atgaggcaag agattttctt gcctcttttg
    12601 aggaggaagc ggtttcggcc tttcgggtca atcccttaaa agaagagcaa ctttcctttt
    12661 ctgatgccat tactcaaacc ccttggggcc actatgggaa ggtttcaggg aaatcgcctg
    12721 agcatgctac aggtttagtt tattcgcaag aacctgctgc tcaaatggtg gctcaagtag
    12781 cccaacccag tcctggtatg aaggtcttgg acttggctgc tgcaccgggg ggcaaatcaa
    12841 ctcaactggc agcctatcta gcaggtgagg ggctacttgt ttccaatgaa atttcaagca
    12901 aacgggctaa gattttggtt gaaaacatgg agcgttttgg agcgacaaat gtcgtggtga
    12961 ccaatgaatc tgccgaccgc ttggtcaagg tctttaaagg ctattttgac ttaatcgttc
    13021 ttgatgcccc ttgctctggt gaaggaatgt ttcgtaagca acctgatgct atggactatt
    13081 ggagcttaga ttatccgagt caatgtgcta gcttgcaaag agaaattctg gaggatgcag
    13141 tgaccatgtt agctgaaggt ggtcgtctgg tttattcgac ctgtacctgg gcacccgagg
    13201 aaaacgaaga gattgtcaat tggttgctgg aagagtatga ttttgacttg ttgccagtag
    13261 agcatatcaa tggaatggta gctggtattg acctgccaga aacggctcgg atgtaccctc
    13321 atcagtttaa gggagagggt cagtttgttg cccatctaca gtttaagggt aacaatccag
    13381 ctcctaaatt taaggcaagt aagagtaacc tcagccgtga acaagttgcc ttgtggcagg
    13441 aatttgccca aaaccatttg aaggtcaatc taccgggtat cttgcagact tttggagacc
    13501 agctttatct cttgccagaa cttttgccag atttagggaa gctcaagatt gctcgcaatg
    13561 gactgcatct gggcactttt aagaagaaac gctttgagcc cagtttcgct cttggtttag
    13621 ccttgaaacc gagtcaggtc gaacagtcgg ttgaaattgg ccaagaagcg tttgtcaagt
    13681 atgcggctgg agaaaccgtt cagctggcag aaagtctgcc aaatggttgg taccaagttt
    13741 tggttaaagg caatggcttg ggctttgcaa aggttactgg aaatgttttg aaaaattatt
    13801 ttccaaaagg cctcagattc aaatgaaaat gctagtcaaa tatcttattc tatgttatag
    13861 tgaaagtatg gattttttta gtaggatgat tcttgatatc ttgattgcca tcacatgtag
    13921 ttttactact cgatgaaaat caaagagcaa actaggaagc tagccataga tagctcaaaa
    13981 cactggtttg aagagatttt caaagagtac aagaagttta gttattagcg ttcttaccgc
    14041 ttgtaaacta gatttctcat aaaatagaat cttttccttt tagttgtaaa ctagtctggg
    14101 agagtagaga ggtttgagat acctttctag cttttggatt atcatctgag gagagtaatt
    14161 tcccttgcat taaaaagggg aaaaagagac acgaaatgat tataatgggt gacaatgggg
    14221 gaagggatag acaagagatt ttatccacat atgaaaaaag gaggttagga aagagtaata
    14281 tagcctatat tatataaata atcaattgcg cagaaatttg gtaagaattc atgcgtcaac
    14341 tcataaagaa ctacttaaaa aattcacagt attcataatt attttcgagg agaaaaacag
    14401 tgaaaaaaag aaaaaagctt gctctgtctc ttatcgcttt ttggctgacg gcttgtttag
    14461 taggctgtgc tagctggatt gatcgtggag aatccataac ggctgttggc tcaactgcct
    14521 tgcaaccctt ggttgaagta gcggcagatg aatttggcac gatccatgtt ggaaaaacgg
    14581 tcaatgtcca agggggaggt tctggtacag gcttgtccca ggttcagtct ggggcagttg
    14641 atataggaaa ctcagatgta tttgctgagg aaaaagacgg aattgatgct tctgctcttg
    14701 ttgaccacaa ggtcgcggta gctggcttgg ctctgattgt caataaggag gttgatgttg
    14761 ataacctaac gacagagcaa cttcgtcaaa tcttcatagg tgaggtaacc aattggaaag
    14821 aggttggtgg taaggactta cccatctctg ttatcaatcg ggcagccggc tctggttctc
    14881 gtgctacctt tgatactgtc attatggaag gtcagtctgc catgcaaagt caggagcagg
    14941 attcaaatgg agcggtaaaa tcaatcgtat caaaaagtcc aggagctatc tcttatttat
    15001 ctcttaccta tatagatgat tcggtcaaaa gcatgaagtt gaatggctat gacttaagtc
    15061 cagaaaatat aagtagcaat aattggccct tgtggtctta tgagcatatg tatacattgg
    15121 ggcagcccaa tgagttggct gcagaatttc tcaattttgt tctctcggat gagacccaag
    15181 aagggattgt caaaggattg aagtatattc cgattaagga aatgaaggtt gaaaaagatg
    15241 ctgccggaac tgtgacagtg ttggaaggga gacaataatg aatcaagaag aattagctaa
    15301 gaaaatgttg cttccatcaa agaattctcg tctggagaaa ttaggaaaag gtttgacctt
    15361 tgcctgtctt tctttgatag tcatccttgt ggccatgatt ttggttttcg tagcgcaaaa
    15421 aggcttgtcg accttctttg tcaatggtgt gaatatcttt gactttcttt tgggaggaac
    15481 ttggaatcct tctagtaaag aatttggtgc ccttcctatg attttgggtt cctttatcgt
    15541 taccattctc tcagccctta tcgcaacacc ctttgctatt ggtgcagcag tttttatgac
    15601 cgaagtatca ccaaaagggg cgaagatttt gcaaccagct attgaactcc tggttgggat
    15661 tccttcagta gtgtatggat ttattggctt gcaagtcgtc gttccctttg ttcgcagtgt
    15721 ctttggtggg actggttttg ggattttgtc agggatttcc gtcctctttg tcatgatttt
    15781 gccgaccgta acctttatga caacggatag cttgcgtgcg gttcctcgtt attatcgtga
    15841 agccagtttc gctatgggag ccactcgctg gcagactatc tggcgtgtga tcttgaaggc
    15901 ggcccgttct ggtattttca ctgcagtggt ctttgggatg gcgcgtgcct ttggtgaggc
    15961 tttagctatc cagatggttg tcggaaactc agctgttatc ccaacttcct tgaccacacc
    16021 agctgcaact ttaacttcta tattaactat gggaattggg aacactgtca tgggaactgt
    16081 aaataataat gttctctggt cactggcctt ggtactgctc ttgatgagtt tagtttttaa
    16141 cagtgtgatt aaattgatta cgaaagaaag aggaaagaaa aattatgcgc gctaagaaat
    16201 tagataaact tgcaacagct gtcctctata cgattgctag catcattgtg acaatcttgg
    16261 cttccttgat tctctatatc ttggttcggg gcttgcccca tatctcttgg tctttcttga
    16321 ctggaaggtc ttctgctttt caagcaggtg gtgggattgg cattcagctt tacaattcct
    16381 ttttcctatt ggtcattacc ttgattattt ctgtacctct ttctatggga gctgggattt
    16441 acttggctga atatgctaaa aaaggtcctg ttaccaactt tgtgcggact tgtattgaaa
    16501 tcttgtcctc tttaccatca gtggtggtgg gtctctttgg ttacttgatc tttgtagtcc
    16561 agtttgagta tggattttca atcatttcag gtgccttggc cttgacagtc tttaacttgc
    16621 ctcagatgac gcgtaatgta gaggatagtt tgaaacacgt tcaccatacc caacgtgagg
    16681 ctggtctggc tcttgggatt tctcgctggg agacagtggt tcatgttgtt attccagaag
    16741 cgcttccagg tattgtaacg ggtgtcgtct tggcatctgg tcgtatcttt ggcgaagctg
    16801 cagctctgat ctatacagca gggcaatcgg cgccagctct tgactggtct aactggaata
    16861 tcctcagtgt gactagcccc atctctatct tccgtcaagc agaaaccttg gctgtccata
    16921 tctggaaagt caatagtgaa ggcactattc cagatggaac cattgtatca gcaggttctg
    16981 ccgctgtgct cctgatcttt atcctgattt ttaactttgg agctcgtaag ttcggaagct
    17041 atctacacaa gaaattaacc gctgcctaaa ggagaagcca tgtcaacata taactgggat
    17101 gagaagcata tccttacctt tcctgaagaa aaagtagccc tttctactaa ggatgtccat
    17161 gtttactatg gtaaaaatga atccattaag gggattgata tgcaatttga aagaaataaa
    17221 attacagctt tgattggtcc gtcgggatcg gggaaatcta cctacttacg cagtctcaat
    17281 cgcatgaatg ataccattga tattgctaaa gtaactgggc agattctcta tcgtggaatt
    17341 gatgtcaacc gtccagaaat caacgtttat gaaatgcgta aacacattgg aatggttttt
    17401 caacgcccca atccatttgc taagtcaatt taccgtaata ttacctttgc gcatgaacgt
    17461 gctggagtta aggataagca agtcctagat gaaatcgtag aaacctccct tcgtcaggct
    17521 gccctctggg atcaggttaa agacgatctc cacaagtcag ccttgacctt atcaggtggt
    17581 cagcaacaac gtctctgtat cgctcgtgcc atctctgtta agccagatat cctcttaatg
    17641 gatgagccag cttcagcctt ggatccgatt gcgaccatgc aactagaaga gaccatgttt
    17701 gagctcaaga aagactttac catcatcatt gtaacgcata atatgcagca ggctgctcgt
    17761 gcaagtgact atacaggctt cttttacttg ggtgatttga ttgagtatga caagactgca
    17821 actattttcc aaaatgccaa gctacagtcc accaatgact atgtatctgg tcactttggt
    17881 tagaaaggaa accgtatgac agatgcgatt ttacaggtat cagacctgtc cgtttattat
    17941 aataaaaaga aggctttgaa tagtgtttcc ctatctttcc aacctaagga aattacagcc
    18001 ttgattggtc catctggatc agggaagtca accctcctca agtctctcaa ccgcatggga
    18061 gatctcaatc cagaggtgac cacaactgga tccgtggtgt acaatggtca caacatctac
    18121 agtccgcgta cagatacggt tgaattacgt aaggaaatcg gaatggtttt ccaacaacct
    18181 aatcctttcc ctatgactat ctatgagaat gttgtctacg ggcttcgtat caatggaatt
    18241 aaggataagc aggttctgga tgaagccgta gaaaaagcct tgcaaggtgc ctctatctgg
    18301 gatgaggtca aggatcgtct atatgattca gctattggat tgtcaggtgg tcaacagcag
    18361 cgtgtctgcg tggcccgtgt cttggcaact agtcctaaaa tcatcctctt ggatgagcca
    18421 acttcggctt tggatccgat ttcagctggt aaaattgagg aaaccttgta tggtctaaaa
    18481 gacaagtaca ccatgcttct ggtaacccgt tccatgcagc aagcttcacg tatctctgat
    18541 aagacaggat ttttcctaga tggagatttg attgaattta atgataccaa gcagatgttc
    18601 cttgatcccc aacacaagga aacggaagac tatattacag gaaaatttgg ataaggagat
    18661 gaaagatgtt acgatctcaa tttgaagaag atttagagaa attacataac cagttctacg
    18721 ctatgggaca agaagtgctc tcacaaatca atcgtacggt acgtgctttt gtcacgcatg
    18781 accgtgacct ggcaaaagag gtcatcgaag atgatgcaga agtaaatgaa tacgaagtga
    18841 aactggaaaa gaaatcattt gaaatgatcg cactccaaca accagtctct caagatttgc
    18901 gtacagtctt gactgtcctt aaggctgtat cagatgtgga gcgtatgggg gatcacgctg
    18961 tagccattgc ccaggcaacc atccgtatga agggggaaga acgcatccca gctgtagagg
    19021 aagaaattaa aaaaatggga cgtgaagtta aaagcgttgt tgaagcagca cttgatcttt
    19081 atcttaatgg ttctgttgac gacgcatacc gggtggcctc catggatgag caaattaacc
    19141 actattttga aactatccgt gaccttgcga ctgaagagat taagaaaaat ccagaagcca
    19201 ttgttacggg tcgtgattat ttccaagtta tttcctactt ggagcgtatc ggtgactatg
    19261 ctaaaaatat ctgtgaatgg gttgtctact ttgaaacagg taagattgtc gaactataaa
    19321 atagtttaat tctgtataaa aaggagcttg aaatcaactg atggctcctt tttttgaacg
    19381 aaaacatatt tttgagataa aaatttaaaa aacacttgac aagtaacttg gatatcgtta
    19441 taattataca aaattaacag agaggttgtt tatttatgaa atcaaaaaaa tggatatttg
    19501 ttttatgtaa ttttcttgca agtttcttct tagtagcttg ccagtcaggt tctaatggtt
    19561 ctcagtcagc tgttgaggcc attaagcaaa aggggaaatt agttgtggca accagtcctg
    19621 actatgcacc ctttgaattt caatcattgg ttgatggaaa gaaccaggta gtcggtgcag
    19681 acatcgacat ggctcaggct atcgctgatg aacttggggt taagttggaa atctcaagca
    19741 tgagttttga caatgttttg accagtcttc aaactggtaa ggctgaccta gcagttgcag
    19801 gaattagtgc tactgacgag agaaaagaag tctttgattt ttcaatccca tactatgaaa
    19861 acaagattag tttcttggtt cgtaaggctg atgtggaaaa atacaaggat ttaactagcc
    19921 tagaaagtgc taatattgca gcccaaaaag ggactgttcc agaatcaatg gtcaaggaac
    19981 aattgccaaa agctcaatta acttccctaa ctaatatggg tgaagcagtc aatgaattgc
    20041 aggctggaaa agtagatgct gttcatatgg atgagcctgt tgcacttagt tatgctgcta
    20101 aaaacgctgg cttagctgtc gcaactgtca gcttgaagat gaaggacggc gacgccaatg
    20161 ccgttgctct tagaaaaaat agtgatgatt tgaaagaagt ggtagacaag gtcatccaaa
    20221 aacttaagaa tgaaggaact taccaaagct atcttgaaaa agcagcaagt ctaactgaag
    20281 ttgaagaata agaaaaagca gagttggatg tcattccaat tctgctttta aatagtttaa
    20341 aacattttcc agattgtcta gggacacctt ggcaaattga taaggacagg tgcttaaata
    20401 ggcctcttca aagaagtcca agcccagttg actgtgtttg agactggcga ttttaggata
    20461 ctgtctcagg tctagcaata aaccatcgtg taggggataa tgaggaaggg gacaaggtgt
    20521 tttagttatc ctttcctcga tttgtttttg gtaagattca tgattggtca agcgagccaa
    20581 acgatttttt tcaaacaatt gactgtcaat atagagtgac agggcctttt gcataatgag
    20641 gttgctgtcg tagtctagga tatttttgta ggccacaaat gcttctttga tagcatttgg
    20701 aagaatgagt gctgtaatac gaagggcagg aaaaaggctg gtcgagaagg acttgatata
    20761 aatgacatgc tcctctgtat caagatagtg gaaggtttgg cccttcttgg agtccaaatc
    20821 acccagataa tcgtcctcta cgatatagac atcatacttg gcagctaagt taagaataga
    20881 tcgtttgtct tgctcagaat aggaatgtcc cagaggatag tgaaatctag gaatggtgta
    20941 gaaaaattta atttttcctg ttttgaagtg gccttccagc tcctccaagt caatcccatc
    21001 aatgcctcgt tcaatcgttt gatagtccag cccctgtgca atcaagaggc gattcatccg
    21061 atggtaggtt ggctgttcca ccaagatttc cttggcttgt ctaggaaagg atatttgaga
    21121 gaggataaac aaggcttgtt gggttccaga agtcagtact agttggttag ccttgcagta
    21181 gagagcttgc tcaaatagga gtttgtgaat ggactgtctt aggtcttcta atccttcttg
    21241 attgtcatag tagttgaaga ggtagttttc tcgtccaatc aaggtttcat tgacacagag
    21301 tcggaaatcg tcataggcac tggcatgttc gtcggtaacc tcgatttcta ggtcttgatg
    21361 ttgcccttgt tctaatacat agtagccact ctgaggcttg gcatagaggt attgttcgtg
    21421 ccgtaattcc agcagggctc gttgaatggt gtccttgctg cagtgaaagt caaggctcag
    21481 ttgacggata gaaggcaggc gactacccgt cggaaatcgt tcagactcga taccattttt
    21541 cagataggaa acgacctctt tgtacttgct ttgtttcttc attccagacc tcccttgatt
    21601 ttgttacatt gtaccctttt ttttccttct tggcaatgtc tagagcgttt ctctcgttca
    21661 catctagggg caaatgtggt atacttagga gtaagattta tagaataaca gacaagaaag
    21721 tgaatggata agaacgtgca ggaaccagtg aaattatttc aatacaatac tcttggagcc
    21781 ttgatggctg gcctttatgg tggtactatg acagtaggag aattgctaga acatggtgac
    21841 cttggtttgg gaaccttgga ttccattgat ggggaattga ttgtcttgga tggcaaggct
    21901 tatcaggcca aagggtcagg agaccagcca gaaattgtgg aagtgtcacc agatgccctt
    21961 attccttacg ctgcagtggt accgcatcag gcagaggtca ttttccgcca gcgctttgag
    22021 atgacagaca aggaattgaa agaacgaatt gagtcttatt atgatgggga aaatcttttc
    22081 cgctctatca agattcgggg ggaattttca catatgcatg tgcgtatgat tcctaagtca
    22141 acacccgata ccaagtttgc tgatgtcgca acccatcaac cggaatatag tcgtgacaat
    22201 gttgcgggga ctattgttgg tttctggacg cctgagattt tccatggggt cagtgtggca
    22261 ggctaccatc tgcacttcat atcagatgat ttgaccttcg gtggacatgt catggatttt
    22321 gtcatcaagg aaggcattat cgaggtggga gcagttgacc agttggacca acgtttccca
    22381 gtccaagacc gtcaatactt gtttgctaag ttcaatgttg acgagatgaa aaaagatatt
    22441 gaaaaggcag aataggagaa gaaaatgacc attcatatca ttattaccat gttgctgttg
    22501 ctagcttttc tgataggaag catttggttt gccaaaaaga aataccagat taatctagct
    22561 gtcttgggct tgggtgcagt cgctttcttt gtctcttcgc agattttgga aaaactggtg
    22621 catatcttga ttttgcatcc tcaaaaagac ggtagtattg ccctcttgca agatcatccg
    22681 cttatctata ttatctatgg cctagccatg gcagcatttt ttgaggaaac ggctcgtctt
    22741 gttttcttca aatggttgga gaaaaagaga agtttggaaa aggcagatgc cttggcctat
    22801 ggcctagggc atggtggctt ggagttgatt tttctaggcc ttattagttt actcaatctt
    22861 tacatcgttc tttcggcagt tcaaactcag aatccacagg ttatgcaatt actgtctgaa
    22921 aatatgttaa aaaccattca gtcactttcg gtctggcaga tttatttgct tggttttgag
    22981 cgaatcttgg cgcttggttt ccaattactt ttgacagttt gggtttacca agctgttcgc
    23041 cagaagaaat ggatttatct cctagcagcc tatggcttgc atgccttctt tgatctggca
    23101 ccatctcttt tccaagtagg ctggttgaca aatccagtct tggttgaagt gattctagca
    23161 ctggagctcg ttctggtcgc ctatggaacc aaggaaatct tttgtaaaaa atcataaaaa
    23221 ggggggaacc tctttttctt atgcaaaatc caaacaaggt atttttatgg tcgtcaaatg
    23281 tctctaaaaa tggtataatg gaatgaattt tgtaaaagga agagtgacat gtctgtaaga
    23341 gaaaaaatgc ttgaaatctt agaaggaatt gatatccgtt ttaaggaacc cttgcatagc
    23401 tatagttata caaaagtagg tggagaggct gattatttgg tctttccacg aaatcgtttt
    23461 gagttggctc gcgttgtgaa atttgccaac caagaaaata tcccttggat ggttcttggc
    23521 aatgcaagca atatcatcgt tcgtgatggt gggattcgtg gatttgtcat cttgtgtgac
    23581 aagctcaata acgtttctgt tgatggctat accattgaag cagaagctgg ggctaacttg
    23641 attgaaacaa ctcgcattgc cctccgtcat agtttaactg gctttgagtt tgcttgtggt
    23701 attccaggaa gcgttggcgg tgctgtcttt atgaatgcgg gtgcctatgg tggcgagatt
    23761 gctcacatct tgcagtcttg taaggtcttg accaaggatg gagaaatcga aaccctgtct
    23821 gctaaagact tggcttttgg ttaccgccat tcagctattc aggagtctgg tgcagttgtc
    23881 ttgtcagtta aatttgccct agctccagga acccatcagg ttatcaagca ggaaatggac
    23941 cgcttgacgc acctacgtga actcaagcaa cctttggaat acccatcttg tggctcggtc
    24001 tttaagcgtc cagtcgggca ttttgcaggt cagttaattt cagaagctgg cttgaaaggc
    24061 tatcgtatcg gtggcgtaga agtgtcagaa aagcatgcag gatttatgat caatgtcgca
    24121 gatggaacgg ccaaagacta cgaggacttg atccaatcgg ttatcgaaaa agtcaaggaa
    24181 cactcaggta ttacgcttga aagagaagtc cggatcttgg gtgaaagcct atcggtagcg
    24241 aagatgtatg caggtggttt tactccctgc aagaggtagt ggggacctga cagagccccg
    24301 atcggttaat ctatgaaaaa gaaggaattt atgccaattg aaaaaaccaa ttatcgaatt
    24361 caaaaacgtc tctaaagttt ttgaagacag caacaccaag gttctcaaag acatcaactt
    24421 tgagttggaa gaagggaaat tctacaccct tctaggtgca tctggttcgg ggaaatcaac
    24481 tatcctaaac attattgcag gtttactgga tgcgacgaca ggagatatca tgctagacgg
    24541 tgttcgtatc aatgatattc caaccaacaa gcgcgacgta cataccgtct tccaatccta
    24601 tgccttgttc ccacatatga atgtgtttga aaatgttgcc tttccacttc gcttgcgtaa
    24661 aattgataag aaagaaattg agcagcgtgt agcggaagtt ctcaagatgg ttcagttgga
    24721 aggttatgaa aaacgttcca tccgcaaact ttctggagga caacgtcagc gtgtggccat
    24781 cgcccgtgct atcatcaacc aaccccgtgt ggtcttgttg gacgagcctt tatcagcgct
    24841 ggacttgaaa ttgagaacag acatgcagta cgaattgcgt gaattacaac aacgattggg
    24901 cattaccttt gtctttgtca ctcacgatca ggaagaagct cttgccatga gtgactggat
    24961 tttcgttatg aatgatggcg agattgtcca gtctggaacc cctgtggaca tctacgatga
    25021 gccaatcaac cactttgttg ccacctttat cggggagtca aacatcttgc caggtaccat
    25081 gattgaggac tacttggtcg aatttaacgg caaacgcttt gaagcggttg atggtgggat
    25141 gaagccaaat gaacctgttg aggtcgttat tcgtccagag gacttgcgca ttacccttcc
    25201 tgaagaaggc aagctccaag ttaaggtcga tacccagctc ttccgtgggg tgcattatga
    25261 aattatcgcc tatgacgaac ttggaaatga atggatgatc cactcaaccc gtaaggccat
    25321 cgtgggtgag gaaatcggtc tggactttga accagaagac atccacatca tgcgtctcaa
    25381 tgaaaccgaa gaagagttcg atgctcgtat cgaagaatac gtggaaatcg aagagcaaga
    25441 agcaggtctg atcaatgcga tcgaggagga aagagatgaa gaaaacaagc tctaaactct
    25501 ttgtagtgcc ctacatgctt tggattgccc tctttgtatt ggcacccttg gtcttgattt
    25561 tcggacaatc ctttttcaac attgaaggcc agtttagttt agaaaattat aaatcttact
    25621 ttgcgtcaca aaacttgacc tacctcaaaa tgagtttcaa ctcagtgctt tatgcaggga
    25681 ttgtgacctt tgtaaccttg cttatcagct atccaacagc cctctttttg acccgtctca
    25741 agcaccgtca actctggctc atgctgatta tccttcctac ctggatcaat ttgctcctta
    25801 aagcctatgc ttttatcggg atttttggtc aaaatggctc tattaaccaa ttcttggaat
    25861 ttatcggaat tggttcacaa cagttgcttt ttaccgattt ttcctttatc tttgtcgcaa
    25921 gctacatcga gctccccttt atgattttgc cgattttcaa tgtcttagac gatatggata
    25981 ataatctcat caatgctagt tacgaccttg gtgcaactaa gtgggagacc ttccgtcatg
    26041 tcatcttccc tctatctatg aacggtgtgc gaagtggggt tcagtcggtc tttatcccaa
    26101 gtttgagtct cttcatgctg acccgtttga ttggtgggaa ccgcgttatc accttgggga
    26161 cggctattga gcagaatttt ctaaccaatg acaactatgg tatgggttca actatcggtg
    26221 tgattctcat cctgaccatg ttcatcacca tgtgggtgac taaggaaagg agagaacgat
    26281 gaaaaaattt gccaaccttt atctgggact ggtctttctg gtcctctacc tgcctatctt
    26341 ttacttgatt ggctatgcct ttaatgctgg tgatgatatg aatagcttta caggttttag
    26401 ctggactcac tttgaaacca tgtttggaga tgggagactc atgctgattt tggctcagac
    26461 atttttcttg gccttcctat cagccttgat agcgaccatt atcgggactt ttggtgccat
    26521 ttacatctac cagtctcgta agaaatacca agaagccttt ctatcactca ataatatcct
    26581 catggttgcg cctgacgtta tgattggtgc tagcttcttg attctcttta cccaactcaa
    26641 gttttcactt ggctttttga ccgttctatc tagtcatgtg gccttctcca ttcctatcgt
    26701 ggtcttgatg gtcttgcctc gactcaagga aatgaatggc gacatgattc atgcggccta
    26761 tgacttggga gctagtcaat ttcagatgtt taaggaaatc atgcttcctt acctgactcc
    26821 gtctatcatt gctggttatt tcatggcctt cacctattcg ttagatgact ttgccgtgac
    26881 cttctttgta acaggaaatg gcttttcaac cctatcagtc gagatttact ctcgtgctcg
    26941 caaggggatt tcgctagaaa tcaatgccct ttctgctcta gtctttctct ttagtattat
    27001 cctagttgta ggttattact ttatctctcg tgagaaggag gagcaagcat gaaaaaaatc
    27061 tattcatttt tagcaggaat tgcagcgatt atccttgtct tgtggggaat tgcgactcat
    27121 ttagatagta aaatcaatag tcgagatagt caaaaattgg ttatctataa ctggggagac
    27181 tatatcgatc ctgaactctt gactcagttt acagaagaaa caggaattca agttcagtac
    27241 gaggcttttg actccaacga agccatgtac actaagataa agcagggtgg aacgacctac
    27301 gatattgcca ttccaagtga atacatgatt aacaagatga aggacgaaga cctcttggtt
    27361 ccgcttgatt attcaaaaat tgaaggaatc gaaaatatcg gaccagagtt tctcaaccag
    27421 tcctttgacc caggtaataa attctccatc ccttacttct ggggaacctt aggaattgtc
    27481 tacaacgaaa ccatggtaga tgaagcgcct gagcattggg atgacctttg gaagctggag
    27541 tataagaatt ctatcatgct ttttgatggg gcgcgtgagg tgctgggact aggactcaat
    27601 tccctcggct acagcctcaa ctccaaggat ccgcagcagt tggaagagac agtggataag
    27661 ctctacaaac tgactccaaa tatcaaggct atcgttgcgg acgagatgaa gggctacatg
    27721 attcagaata atgttgcaat cggcgtgacc ttctctggtg aagccagcca aatgttagaa
    27781 aaaaatgaaa atctacgtta tgtggtaccg acagaggcca gcaatctttg gtttgacaat
    27841 atggtcattc ccaaaacagt taaaaatcaa gactcagcct atgcctttat caactttatg
    27901 ttgaaacctg aaaatgctct ccaaaatgcg gagtatgtcg gctattcaac accaaaccta
    27961 ccagcgaagg aattgctccc agaggaaaca aaggaagata aggccttcta tcccgatgtt
    28021 gaaaccatga aacacctaga agtttatgag aaatttgacc ataaatggac agggaaatat
    28081 agcgacctct tcctacagtt taaaatgtat cggaagtagg agataaacgt aagaagacga
    28141 atcagtcaag ctggttcgtt tttaaggaat agaagtcagt ttttagagcc ctatcttttt
    28201 ctattctagt tgtttatttg tttatttgct tatttgcttg cctagtagta tactaagcct
    28261 atactcttca aaccacgtca gcgttgcctt gccgtactca agtacagctt gaagctagct
    28321 tcctagtttg ctttttgatt tttattgagt attaacctta cagaaagcgg caacaaataa
    28381 tgaaaatgtc tacttttttc aaaaaaagtt tctggccaac ctttatgatt gttaaccaaa
    28441 ctgctatact attccattta aatgatggct tggatcgaca atacctaaca actgagtcca
    28501 tatattgggt gataggcacc tttatatttg gaaatattct agtagctgtg tttagtaata
    28561 tgaaaatttg ggataaaaag aaaaatggca gtaagaaaaa atatatcctg aaaaagtaat
    28621 cagagatgta gggaattggt ctaattcaac ggagatcatt tccagttgac gcaagcttaa
    28681 aaaaacgtta taataagaaa gttgagaatt gattttcact tgtcttagtc cagagaaatg
    28741 gcggtgctgc gagccatcta agctaggaag tcatgctact caatcataca attgcataag
    28801 aacaagagaa tgacaagttc attgaatgaa ggtggtaccg cggtttttcg cccttcgtga
    28861 tatgagcttg tcttttgatt tttggaggtg ttgatgaaaa catttcttgt caaacaaaag
    28921 tttcgtcttg gaggcgaacg cttcgctatc aaggatgaca ggggagaaat tgcctatcag
    28981 gtggagggat cattttttaa gattcccaaa acttttacca tctatgatgc ggctggtgaa
    29041 caagtcagtc agatcagtaa agaaatcttg accttgcttc ctcgttttga gattcagctt
    29101 cgggatggct cgagttttgt cattcgtaag aagttgacct tctggcgaga taagtatgag
    29161 tttgataatc taggtcttcg tatcgagggc aatatctggg atttgaattt caaattgctg
    29221 gatgatcgcg atcagttgat tgcggaaatt aagaaggaac tcttccatct gacctctacc
    29281 tataccgtaa cggttcttga agacgcttat gcagacctag tcatttccct ctgtgtcgcg
    29341 attgactatg tggaaatgct ggaaagccaa tcacattaaa caagtgaata aggagacaat
    29401 atgaaacaac tatctagtgc acaagtacgc caaatgtggc ttgatttctg ggcgaccaaa
    29461 ggtcactcag tagaaccatc agtaagtttg gttcctgtaa atgacccaac tcttttgtgg
    29521 atcaactctg gggtagcaac ccttaagaaa tactttgacg ggaccattat ccctgaaaat
    29581 ccacgtatta ccaatgccca aaaggctatc cgtaccaacg acatcgaaaa cgtagggaag
    29641 actgcgcgtc accataccat gtttgaaatg ctggggaact tctctatcgg ggattacttc
    29701 cgtgacgaag ctatcacttg ggcttatgag cttttgacaa gccctgaatg gtttgatttc
    29761 cctgctgaaa aactttacat gacctactat ccagacgata aagattctta caaccgctgg
    29821 attgaagtgg gagtggatcc aagtcacttg attccaattg aggacaactt ctgggaaatc
    29881 ggtgcgggac cttctggacc agatacagaa atcttctttg accgtgggga agcctttgac
    29941 ccagaaaata tcggtcttcg cctgcttgca gaagatattg aaaacgaccg ttatattgaa
    30001 atctggaaca tcgttttgtc acaatttaac gcagaccctg ctgttcctcg tagcgaatac
    30061 aaggaattgc cacataagaa cattgatacg ggcgctggtt tggagcgttt ggtggccgtt
    30121 atccaagggg ctaagaccaa ctttgaaacg gacctcttca tgccgattat ccgtgaagtc
    30181 gagaaattgt ctggtaaggt ttatgaccaa gatggcgaca acatgagctt caaggttatc
    30241 gcagaccaca ttcgttctct ttcatttgct atcggtgatg gtgcccttcc aggaaatgaa
    30301 ggtcgtggtt atgttcttcg tcgtcttctc cgtcgtgctt ctatgcatgg tcaaaaattg
    30361 ggtatcaacg agcctttcct ttacaaattg gttccaactg ttggaaaaat catggaaagc
    30421 tactacccag aagtgcttga gaaacgtgac tttattgaga aaatcgttaa gagcgaagaa
    30481 gaatcatttg cccgtaccct tcactcaggt caacactttg cccaaggcat tgtggcagac
    30541 ttgaaagaaa aaggtcaatc tgtcattgct ggtcaagatg tctttaaact ctatgatact
    30601 tatgggttcc cagttgaatt gactgaagaa atcgctgaag aagctggtat gactgtagac
    30661 cgtgaaggtt ttgaagcagc catgaaagaa cagcaagaac gcgcgcgtgc gtcagctgtc
    30721 aagggtggct caatgggtat gcaaaatgaa actcttcaaa acatcactgt agaaagtgtc
    30781 ttcaactaca atactagcca attgtcttct aaattggtag ctatcgttgc tgacaatgca
    30841 gaagtaggag ctgtttcaga aggaactgcc tctcttatct ttgcggaaac gtcattttat
    30901 gctgaaatgg gtggacaggt agctgactac ggacaaatct tggatgagtc aggtaagatt
    30961 gtggctactg tgaccaatgt tcagaaagcc ccaaatggtc aagcccttca tacagttgaa
    31021 gtccttgcac cgcttgcctt gaaccaagaa tataccttgg caattgatag caatcgccgt
    31081 caccgtgtca tgaaaaacca cactgcgact catttgcttc acgctgccct tcacaatatc
    31141 cttggaaacc acgcaacaca ggcaggatct cttaacgaag ttgaattcct tcgctttgac
    31201 tttacccact tccaagcagt aactgctgag gaacttcgtg cgattgaaca gcaagttaac
    31261 gagaaaattt gggaagcact tgaagtgaag acagttgaaa cggatattga cactgctaaa
    31321 gaaatgggag ctatggccct ctttggtgag aaatacggca aggaagttcg tgtcgtgact
    31381 atcggtgact actctattga actttgtggt ggtactcatg ttgacaacac ttctgagatt
    31441 ggtctcttca aaattgtcaa agaagaagga atcggttcag gaactcgccg tatcttggca
    31501 gtgactggta aggaagcctt tgaagcctat cgtgaacaag aggatgctct taaagctatc
    31561 gcagcaacct tgaaagcacc tcaagtcaag gaagtacctc acaaggtaga aggacttcaa
    31621 gaacaacttc gtcaacttca aaaagaaaat gctgagttga aagaaaaagc cgcagctgca
    31681 gccgcaggcg atatcttcaa agatgttaag gaagtcaacg gtcatcgtta cattgctagt
    31741 caagtgtctg tatccgatgc cggtgccctt cgtacttttg cagataactg gaaacaaaaa
    31801 gactactctg atcttcttgt cctagttgcc gctatcggtg acaaagtcaa tgtccttgta
    31861 gcaagcaaga caaaagacct tcatgcagga aaccttgtca aagaattagc accaatcatc
    31921 gatggacgtg gtggtggtaa accagacatg gccatggcag gaggaagcaa ccaagctaag
    31981 atccaagaat tgttggatgc agtagcaggt aaattgtaat caagtaaaga tctatccatt
    32041 tgggtaggtc tttttgtgaa tacaaaaaag ccaaatccgc ttggacctag cttggttatt
    32101 atgagctatt agatggtatt gactgcccag acacttacgg atctagctga gacagggaat
    32161 tgtccataac cttcctcatc aattgtaact tgaccttggt ggttaccaag taaatctaca
    32221 aaggtttgat tagtccattc ttgaccgaca aacattgact tgctgttttc ttggtcattt
    32281 gagataagga ctgcgattgg ggattgattt tcagcacctg aacgtaccca accgatacag
    32341 ttagcatggt caaagtagtc attttgttct ccataggcca agtcttttcg gatggctagg
    32401 aggcggtcaa ggatttcttt gaaatcttct tgagcatact ggcctgaaat cccatagtag
    32461 tctccgtaaa agacacatgg aaggccgtct tggcgtaaca aaatgagggc ataggctgct
    32521 ggcttgaacc attcttcaac ggtagactca agggcttgtc ctcgttgggt atcgtggttg
    32581 tcgacaaaag tcacagcctt gtcaggcttg agttcaacca ggctatctgt gaaaatgcca
    32641 cgaaggtcat agtttgcgcc agcttgactg gcttcaaaga gattctggtg gagacgaaca
    32701 tcgacaaggt caaagtgttc ttccgttttt tcgagatagt ccagattggc ttccttgtct
    32761 gggttccaaa attcaccaaa aacatagaaa tcgtcaccgt atttttcctt catatcgcgg
    32821 atgaagttgc gcataaagaa agagtcaata tgcttaacgg catccaaacg gaaaccagct
    32881 acaccagtcg tttccatgaa ccaatcagcc cagtcataga tgttttggat gacttcagga
    32941 tgtttaaagt ctaggtcggc atacatgagg tagtcgtagt ttccgttttc gttatcgacc
    33001 aattccttgt tagcccagcc cttgttgtcc ccttggatca gataaatccc agatttactg
    33061 cgtttggcat cgtagtctgt accggtgaag tggtaccaat gccagtggaa gccattatag
    33121 gtatcttggc gaccatcgaa ggtaaaacta gtccagccat tgatggtgaa gggttctcca
    33181 agttcaactg tacggtctac aggatcaact tcgataacct gaaaggcttc cctgttatcg
    33241 gcagcagcct tgtggttgag aactacatcg gccataggtt gaattccctg ttctttaagg
    33301 gcttgaatgg cttgaagata gtcttctttg aaaccatact tggtgcggac agtccctttt
    33361 tggttgaact ctcctaagtc aaataagtca tagaccccat agccgacatc tttttcgttg
    33421 gttgccttga aggctggtgg catccagacg tgactgatcc ccagatgagc taggtgtgga
    33481 gcattttcag ccagacgcgt ccagtgttga ccgtcgtggg gcagatacca ttcaaagtat
    33541 tgcatgagtg tttgattttg catgatgttt cctcttactt gtcaatcttg ttctttattc
    33601 tatcataaag tatctctcgg cgcaaacgtt tgcgtaagat agaaggaaac tattttaact
    33661 acttaaaata aagtattgat ttttttgtat caaagtgcta gtatagcgat gtataataca
    33721 gataaagata ggagttgtcc catgattgaa tttcaaaatg ttagtaagtt gtatggtgat
    33781 aaagaggcct tgagtaatct caatttgcag attgaaaatg gagagattat gggcttgatt
    33841 ggtcataatg gggctggaaa atcgaccacc ataaaatccc tagtcagtat catttcaccc
    33901 agcagtggtc gtattttggt agacggtcag gagttatcgg aaaatcgctt ggcgatcaaa
    33961 cgaaagattg gctacgtagc agactcgcct gacttatttt tacgcttaac ggccaatgaa
    34021 ttttgggaat tgatcgcttc atcctatgat ctgactagct ctgacttgga gactagtcta
    34081 gctaggctat tgaacgtttt tgattttgct gaaaaccgct atcaggttat tgaaactctt
    34141 tctcacggaa tgcgtcagaa agtctttgtc attggggcac tcttgtctgc tcctgatatt
    34201 tgggttttgg acgaaccctt gactggtttg gatccccagg ccgcctttga tttgaagcaa
    34261 atgatgaagg aacatgcaca aaaaggcaag acggtcttgt tttcaactca tgtccttgag
    34321 gtagccgagc aagtctgtga tcggattgct attttgaaaa aggggcattt gatctattgt
    34381 ggtagtgtag agaacttgag gaaagaccac ccagaccagt ttttggaaag tatctacctt
    34441 agtcttgctg gtagaaaaga ggaggttgcg gatgcgtctc aaggtcatta aaaaattagt
    34501 tgatatcaat atcctctatt catctcaaga agctaatctg gctaatctac gaaagaagca
    34561 ggctaagaat cctgggaaaa aagtaaatgt ttctgctaga gtcctaagtt cttacatttt
    34621 ttccagtctc ttgatgctca tctgttttag taatatagcc attcattttc cttttgagga
    34681 aatgcctatt tattttagct cgatggttgc tattttactg gtgctggcct tttcaacttc
    34741 tttaacggcc ttttacaatg tcttttatga gagtaaggac ctggcctcct ataggcccta
    34801 tgcctttaaa gaatcagaga ttataatggc caaaggtctg tctgtcctct tgccagctct
    34861 aactggaatt gtaccaatcc tagcttattt tctagccctc tatattagtc tagctccttc
    34921 tctttggctg ggtttgcctt tgatgctact gtccttgacc ttattatttg tctctgttac
    34981 tttagtgacg gtagtggcag tacatttctt ggctcagact agggtcttca gaaagtatca
    35041 gtctattttt tcgaatgtga tgattgggat aggagttctc atacctttaa tatttatctt
    35101 ctttcttcag tcgacttttg gaagtattgt tgacaaagtt agagacattc catttctcct
    35161 ttatcctctt catatctttt acaaaatagc agtggagcct ttttcgacag aagccttagt
    35221 gggtctgctc gcttggatag gactaactct cttcctgctt tatctgacca aaaagaaggt
    35281 ccttcctcgt ttttatgacg tgatcctgct taacagtgag gagaaggtca aaaaagaacg
    35341 tcgcagcaag gagaggattt caactactaa aaagggcttt ttccgtatgg ttttacgcta
    35401 ccacctcacc ctcttgggac aggggactgg tgtgattaca gtgcttttta caagtgcttt
    35461 ccttccttat ctcatgatga tcagtctgat ttccaaaatc cgagattctc agatagttcc
    35521 agacattcat cctccatact ggttaccctt gttttttgta ggagtgttta tagcagttgt
    35581 caataacaat atcaccagcc tgcattcaat tgccttgtcc ttggagaggg aaaatgttga
    35641 ttttctgaag agtttaccct ttgactttgc tcgttatgtg aaagtgaaat tttggattat
    35701 ctttgctgtt cagtcctttt taccagttct gactttgctc ggtctttctc tatatctagg
    35761 cttgcccatc ctttcgatga tttaccttct tgtagtctgg atcattgcca gtgtcatcct
    35821 ttcttgccac cattacttta aggacgttaa aaatctgtca accaattgga gtagcattac
    35881 ggacctggtg aatcgttcaa atggcatagt cgccatagtt ttattgttta tttatagtgc
    35941 aatcctgatg gcccttgtaa ttgggagcat attcttggtt cagtctctct cccctatcct
    36001 tgccatcaac ttgggagtag gagctcttat cgtcttgctt gctcttgcta tttttggcta
    36061 tcattattac ctgtcacgca tattggcaga aatagaaaaa agatgagata gttggttgct
    36121 tgcttgataa attcaattcg acaaacgctt ttatagtttg ttagaatgta gttgtaaatg
    36181 attaagattg gtaatcaaaa atatagggag gaatgttttg aataagaaat ataatatag
//
LOCUS       DAVLRT010000013        34397 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLRT010000013 DAVLRT010000000
VERSION     DAVLRT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.013"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            571..1023
                     /locus_tag="VXM85_000640"
     CDS             571..1023
                     /locus_tag="VXM85_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1345265.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1042..2379
                     /locus_tag="VXM85_000641"
     CDS             1042..2379
                     /locus_tag="VXM85_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1345266.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2458..3342
                     /locus_tag="VXM85_000642"
     CDS             2458..3342
                     /locus_tag="VXM85_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1345267.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3342..4175
                     /locus_tag="VXM85_000643"
     CDS             3342..4175
                     /locus_tag="VXM85_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1345268.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWSEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4193..6286
                     /gene="nanB"
                     /locus_tag="VXM85_000644"
     CDS             4193..6286
                     /gene="nanB"
                     /locus_tag="VXM85_000644"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1345269.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6298..7401
                     /locus_tag="VXM85_000645"
     CDS             6298..7401
                     /locus_tag="VXM85_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1345270.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7575..8273
                     /locus_tag="VXM85_000646"
     CDS             7575..8273
                     /locus_tag="VXM85_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1345271.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8292..9809
                     /locus_tag="VXM85_000647"
     CDS             8292..9809
                     /locus_tag="VXM85_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1345272.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFLGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9934..11262
                     /locus_tag="VXM85_000648"
     CDS             9934..11262
                     /locus_tag="VXM85_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1345273.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11514..12380
                     /locus_tag="VXM85_000649"
     CDS             11514..12380
                     /locus_tag="VXM85_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1345274.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12396..13235
                     /locus_tag="VXM85_000650"
     CDS             12396..13235
                     /locus_tag="VXM85_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1345275.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13508..13960
                     /locus_tag="VXM85_000651"
     CDS             13508..13960
                     /locus_tag="VXM85_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1345276.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14057..14401
                     /locus_tag="VXM85_000652"
                     /pseudo
     CDS             14057..14401
                     /locus_tag="VXM85_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14678..15322
                     /locus_tag="VXM85_000653"
     CDS             14678..15322
                     /locus_tag="VXM85_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1345277.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSAFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15491..16408
                     /locus_tag="VXM85_000654"
     CDS             15491..16408
                     /locus_tag="VXM85_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1345278.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKESFL"
     gene            16426..17310
                     /locus_tag="VXM85_000655"
     CDS             16426..17310
                     /locus_tag="VXM85_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1345279.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17326..18177)
                     /locus_tag="VXM85_000656"
     CDS             complement(17326..18177)
                     /locus_tag="VXM85_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1345280.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18377..20434
                     /gene="pbp2b"
                     /locus_tag="VXM85_000657"
     CDS             18377..20434
                     /gene="pbp2b"
                     /locus_tag="VXM85_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1345281.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20445..21041
                     /gene="recR"
                     /locus_tag="VXM85_000658"
     CDS             20445..21041
                     /gene="recR"
                     /locus_tag="VXM85_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1345282.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21217..22260
                     /locus_tag="VXM85_000659"
     CDS             21217..22260
                     /locus_tag="VXM85_000659"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1345283.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLVYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPKLIERLVDL
                     AKESFDKREAHLI"
     gene            22344..23717
                     /locus_tag="VXM85_000660"
     CDS             22344..23717
                     /locus_tag="VXM85_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1345284.1"
                     /translation="MKLTIHEIAQVVGVKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLMP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23704..24315
                     /locus_tag="VXM85_000661"
     CDS             23704..24315
                     /locus_tag="VXM85_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1345285.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTGNILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24406..25110
                     /locus_tag="VXM85_000662"
     CDS             24406..25110
                     /locus_tag="VXM85_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1345286.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25329..26702
                     /gene="ftsA"
                     /locus_tag="VXM85_000663"
     CDS             25329..26702
                     /gene="ftsA"
                     /locus_tag="VXM85_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1345287.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26719..27978
                     /gene="ftsZ"
                     /locus_tag="VXM85_000664"
     CDS             26719..27978
                     /gene="ftsZ"
                     /locus_tag="VXM85_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1345288.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27983..28654
                     /locus_tag="VXM85_000665"
     CDS             27983..28654
                     /locus_tag="VXM85_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1345289.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28664..29203
                     /locus_tag="VXM85_000666"
     CDS             28664..29203
                     /locus_tag="VXM85_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1345290.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29203..29466
                     /locus_tag="VXM85_000667"
     CDS             29203..29466
                     /locus_tag="VXM85_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1345291.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29463..30248
                     /locus_tag="VXM85_000668"
     CDS             29463..30248
                     /locus_tag="VXM85_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1345292.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEYL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30257..31045
                     /gene="divIVA"
                     /locus_tag="VXM85_000669"
     CDS             30257..31045
                     /gene="divIVA"
                     /locus_tag="VXM85_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1345293.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31393..34185
                     /locus_tag="VXM85_000670"
     CDS             31393..34185
                     /locus_tag="VXM85_000670"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1345294.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIVEGPAPEATLSFEDVVFTVERAAGEVCDRCRRIDPTTAERSYHAVICDHCASI
                     VEENFADAVAEGFEEK"
BASE COUNT        10591 a         6069 c         7466 g        10271 t
ORIGIN      
        1 ttgaaaacgg taacaaaact aatttagaga atgaaatata gagtatttct ctcttaaaag
       61 tttttggtga aacgagatgt agaaaggaga tttagccaaa gagtctatta gtgctagaat
      121 aatagattag aattatttta gaaaaacgaa gtgagcagct tataaattca agtccccaaa
      181 tagattcata ctagtatctt ttgcaaaaaa taaagggcga cttccttcat gaatatcaat
      241 ttcatctata aggaaggtag ctaattgaac taacttattt attctgtttg tcgctagaaa
      301 aatcagacct ccttgtgaag attgaggaga tacttaatga aaatcaaaga agaaactagc
      361 aagctagtag cagattgccc aaaacaccgc tttgaggttg tagataagac tgacgtatat
      421 aatccaaggt gaagcgactg tggtttgaag agattttcaa agagtatagg ctagagagta
      481 gtgtttttat gtccttctag tagaaaatgc tagacagaag aatggggaac ttggatagga
      541 aaaatagatt gagaaaggag gttagaagag atgattatta caaaaattag ccgtttagga
      601 acttatgtgg gagtaaatcc acattttgca acattaatag attttctaga aaaaacagga
      661 ctagaaaatt taacagaagg ttcgattgct atcgatggta atcgattgtt tgggaattgc
      721 tttacttatc tagcagatgg tcaagcaggg gctttctttg aaacacacca aaaatatttg
      781 gatattcatt tagttttgga aaacgaagaa gccatggctg ttacatcgcc ggaaaatgta
      841 agcgttaccc aagaatatga tgaagagaaa gatattgaat tatacacagg gaaagtggaa
      901 cagttggttc atttgagagc tggtgaatgc ctcatcactt ttccagaaga tttacatcaa
      961 cccaaggttc gtataaatga tgaacctgtg aaaaaagttg tctttaaagt tgcgatttct
     1021 taatgtagaa agagaagaac gatgaaaaaa atgagaaagt ttttatgtct agctggaatt
     1081 gcgctagcgg ctgttgcctt ggtagcttgt tcaggaaaaa aagaagctac aactagtact
     1141 gaaccaccaa cagaattatc tggtgagatt acaatgtggc actcctttac tcaaggaccc
     1201 cgtttagaaa gtattcaaaa atcagcagat gctttcatgc aaaagcatcc aaaaacgaaa
     1261 atcaagattg aaacattttc ttggaatgac ttctatacta aatggactac aggtttagca
     1321 aatggaaatg tgccagatat cagtacagct cttcctaacc aagtaatgga aatggtcaac
     1381 tcagacgctt tggttccgct aaatgattct atcaagcgta ttggacaaga taaatttaac
     1441 gaaactgcct taaatgaagc aaaaatcgga gatgattact actctgttcc tctttattca
     1501 catgcacaag tcatgtgggt tagaacagat ttgttaaaag aacataatat tgaggttcct
     1561 aaaacttggg atcaactcta tgaagcttct aaaaaattga aagaagctgg agtttatggc
     1621 ttgtctgttc cgtttggaac aaatgactta atggcaacac gtttcttgaa cttctacgta
     1681 cgtagtggtg gaggaagcct cttaacaaaa gatcttaaag cagacttgac aagccaactt
     1741 gctcaagatg gtattaaata ctgggttaaa ttgtataaag aaatctcacc tcaagattct
     1801 ttgaacttta atgtccttca acaagctacc ttgttctatc aaggaaaaac agcatttgac
     1861 tttaactctg gcttccatat cggaggaatt aatgccaaca gtcctcaatt gattgattcg
     1921 attgatgctt atcctattcc aaaaatcaaa gagtctgata aagatcaagg aattgaaacc
     1981 tcaaacattc caatggttgt ttggaaaaat tcaaaacatc cagaagttgc taaagcattc
     2041 ttagaagcac tttataatga agaagactac gttaaattcc ttgattcaac tccagtaggt
     2101 atgttgccaa ctattaaggg gattagcgat tctgcagcct ataaagaaaa tgaaactcgt
     2161 aagaaattta aacatgctga agaagtaatt actgaagctg ttaaaaaagg tactgctatt
     2221 ggttatgaaa atggaccaag tgtacaagct ggtatgttaa ctaaccaaca cattattgaa
     2281 caaatgttcc aagatatcat tacaaatgga acagatccta tgaaagcagc aaaagaagca
     2341 gaaaaacaat taaatgattt atttgaggct gttcagtaga tgtaaaagac tagaaaatag
     2401 gtgggctagt gagctgaaaa gctctagccc aatcttgtaa aagaagggag aaggagaatg
     2461 gttaaagaac gtaatttaac tcgctggata tttgttttgc cagctatgat tatcgtagga
     2521 ttactctttg tttatccgtt tttctcgagt attttttata gctttaccaa taagcatttg
     2581 attatgccta attataaatt tgttggtttg gctaactata aagctgtgct atcagatccc
     2641 aacttcttta atgcgttctt taattcaatt aagtggaccg ttttctcatt agttggtcaa
     2701 gttttagtag ggtttgtatt ggctttagct cttcacagag tacgccactt caagaaatta
     2761 tataggacct tattgattgt tccttgggca tttcctacca tcgttattgc cttttcttgg
     2821 cagtggattc taaacggggt ttatggctac ttacctaatc taatcgtaaa attaggttta
     2881 atggaacata cacctgcatt tttgacagat agtacatggg cattcctatg tttggtgttt
     2941 atcaacattt ggtttggagc accaatgatt atggttaatg tgctttcagc tttgcaaaca
     3001 gtaccagaag aacaatttga ggctgctaag atagatggtg cttcaagttg gcaggtgttc
     3061 aagtttatcg tctttccaca tattaaagtg gttgtaggac ttctagttgt tttgagaact
     3121 gtatggatct ttaataactt tgacattatc tacctcatta ctggtggtgg accagccaat
     3181 gctacaacga cgcttccaat ttttgcttac aacctgggtt ggggaactaa attgttgggt
     3241 cgtgcttcag cagttacagt attgctcttt atcttcttgg tggcgatttg ctttatctac
     3301 tttgctatca tcagtaagtg ggaaaaggag ggtagaaaat aatgaagaag aaatccagta
     3361 tttatttaga tattctctca catgtacttt tagttggtgc gaccatcgtt gcaattttcc
     3421 cattggtatg gattatcata tcttctgtca aagggaaagg ggaattaact cagtatccaa
     3481 cacgattttg gtctgaacag tttacattag attatttcac tcatgttatc aacgatttgc
     3541 acttcattga taacattcga aacagtttaa tcattgcctt ggctacaacc ctcattgcga
     3601 ttattatttc tgctatggca gcctatggta ttgttcgatt ctttcctaaa ttgggagcaa
     3661 tcatgtcgag actactcgtc attacctaca ttttcccacc aattttgtta gcaattccct
     3721 attcaattgc cattgctaaa gttgggttaa caaatagttt atttggcttg atgatggttt
     3781 atctatcttt tagtgttcca tatgcagttt ggctcttagt tggatttttc caaacagttc
     3841 caattggaat tgaagaagcg gctagaattg atggtgcaaa taaatttgtt acgttttata
     3901 aagttgtgct accgattgta gcaccaggta ttgtagcaac agctatttat acatttatca
     3961 atgcttggaa tgaattcctg tatgccttga ttttgattaa caatacagga aagatgacag
     4021 tagcagtagc ccttcgttca cttaatggtt cagaaatact agactgggga gatatgatgg
     4081 cagcgtctgt tattgtagtt cttccatcaa ttattttctt ctctatcatc caaaataaga
     4141 ttgcaagtgg attatcagaa ggatctgtga agtagacgaa agaaggaaaa aaatgaataa
     4201 aagaggtctt tattcaaaac taggaatttc tgttgtaggc attagtcttt taatgggagt
     4261 tcccactttg attcatgcga atgaattaaa ctatggtcaa ctgtccatat ctcctatttt
     4321 tcaaggaggt tcatatcaac tgaacaataa gagtatagat atcagctctt tgttattaga
     4381 taaattgtct ggagagagtc agacagtagt aatgaaattt aaagcagata aaccaaactc
     4441 tcttcaagct ttgtttggcc tatctaatag taaagcaggc tttaaaaata attacttttc
     4501 aattttcatg agagattctg gtgagatagg tgtagaaata agagacgccc aaaagggaat
     4561 aaattattta ttttctagac cagcttcatt atggggaaag cataaaggac aggcagttga
     4621 aaatacacta gtatttgtat ctgattctaa agataaaaca tacacaatgt atgttaatgg
     4681 aatagaagtg ttctctgaaa cagttgatac atttttgcca atttcaaata taaatggtat
     4741 agataaggca acactaggag ctgttaatcg tgaaggcaag gaacattacc tcgcaaaagg
     4801 aagtattgat gaaatcagtc tatttaacaa agcaattagt gatcaggaag tttcaaatat
     4861 tcccttgtca aatccatttc agttaatttt ccaatcagga gattctactc aagctaacta
     4921 ttttagaata ccgacactat atacattaag tagtggaaga gttctatcaa gtattgatgc
     4981 acgttatggt gggactcatg attctaaaag taagattaat attgccactt cttatagtga
     5041 tgataatggg aaaacgtgga gtgagccaat ttttgctatg aagtttaatg actatgagga
     5101 gcagttagtt tactggccac gagataataa attaaagaat agtcaaatta gtggaagtgc
     5161 ttcattcata gattcatcca ttgttgaaga taaaaaatct gggaaaacga tattactagc
     5221 tgatgttatg cctgcgggta ttggaaataa taatgcaaat aaagccgact caggttttaa
     5281 agaaataaat ggtcattatt atttaaaact aaagaagaat ggagataacg atttccgtta
     5341 tacagttaga gaaaatggtg tcgtttatga tgaaacaact aataaaccta caaattatac
     5401 tataaatgat aagtatgaag ttttggaggg aggaaagtct ttaacagtcg aacaatattc
     5461 ggttgatttt gatagtggct ctttaagaga aaaacataat ggaaaacagg tccctatgaa
     5521 tgttttctac aaagattcgt tatttaaagt gactcctact aattatatag caatgacaac
     5581 tagtcagaat agaggagaga gttgggaaca atttaagttg ttgcctccgt tcttaggaga
     5641 aaaacataat ggaacttact tatgtcccgg acaaggttta gcattaaaat caagtaacag
     5701 attgattttt gcaacatata ctagtggaga actaacctat ctcatttctg atgatagtgg
     5761 tcaaacatgg aagaaatcct cagcttcaat tccgtttaaa aatgcaacag cagaagcaca
     5821 aatggttgaa ctgagagatg gtgtgattag aacattcttt agaaccacta caggtaagat
     5881 agcttatatg actagtagag attctggaga aacatggtcg aaagtttcgt atattgatgg
     5941 aatccaacaa acttcatatg gcacacaagt atctgcaatt aaatactctc aattaattga
     6001 tggaaaagaa gcagtcattt tgagtacacc aaattctaga agtggccgta agggaggtca
     6061 attagttgtc ggtttagtca ataaagaaga tgatagtatt gattggaaat accactatga
     6121 tattgatttg ccttcgtatg gttatgccta ttctgcgatt acagaattgc caaatcatca
     6181 cataggtgta ctgtttgaaa aatatgattc gtggtcgaga aatgaattgc atttaagcaa
     6241 tgtagttcag tatatagatt tggaaattaa tgatttaaca aaataaagga gaaaaacatg
     6301 gttaaatacg gtgttgttgg agcagggtat tttggagctg aattggctcg ctacatgcaa
     6361 aagaatgatg gagcagagat tactcttctc tatgatccag ataatgcaga ggcgattgca
     6421 gaagaattgg gagcaaaagt agcaagttcc ttagatgagt tggtttctag cgatgaagta
     6481 gattgtgtta tcgtcgcaac tccaaataat cttcataagg aaccggttat taaggctgca
     6541 cagcatggta aaaatgtttt ctgtgaaaaa ccaattgcgc tttcttatca agattgtcgc
     6601 gagatggtag atgcgtgtaa agaaaacaat gtaaccttta tggcaggaca tattatgaat
     6661 ttctttaatg gtgttcatca tgcaaaagaa ctcattaatc aaggagttat cggagacgtt
     6721 ctatattgtc atacagctcg taatggttgg gaagaacaac aaccgtcagt atcatggaaa
     6781 aaaattcgtg aaaaatcagg tggtcacttg tatcaccaca tccatgaatt ggattgcgtt
     6841 caattcctta tggggggcat gcctgaaact gtaaccatga caggtggaaa tgtggcccat
     6901 gaaggtgaac atttcggtga tgaagatgat atgatttttg tcaatatgga attttctaat
     6961 aagcgttttg ctttgttaga atggggttca gcttatcgtt ggggtgaaca ttatgtctta
     7021 atccaaggaa gcaaaggtgc catccgctta gacttattca actgtaaagg aactcttaag
     7081 ctagatgggc aagaaagcta tttcttgatt cacgaatcgc aagaagaaga tgatgatcgg
     7141 actcgtatct atcatagtac agagatggat ggagcaattg cttatggtaa accaggtaaa
     7201 cgtactccat tatggctatc atctgtcatt gataaagaaa tgcgctatct gcatgagatt
     7261 atggaaggag ctccagtatc agaagaattt gcaaaacttt tgacaggtga agctgcccta
     7321 gaagcaattg ctactgcaga tgcttgtacc cagtctatgt ttgaagatcg caaagtaaaa
     7381 ttgtcagaaa ttgtaaaata aattttggta ttctcctatt tataggtcga cttgctcctc
     7441 tgaaagtact tttagaggag ctgtttgact ttgctagttt ttgaaactga aatctattat
     7501 actacaaact attgaaagcg ttttaatttt aaggtataat aatctcatag aaataaagaa
     7561 aaggaggaaa gaggatgcca cagattagca aagaagcctt gattgagcaa atcaaagatg
     7621 gaatcatcgt ttcttgtcag gctcttcctc atgaaccgct ttatacagaa gcgggagggg
     7681 tgattccctt gctggtcaaa gcggctgagc aaggtggagc agtcggtatc cgagcaaaca
     7741 gtgttcgcga tatcaaggaa attaaggaag tcactaaact tccaatcatt gggattatca
     7801 aacgtgatta tccacctcag gaacccttca tcacggctac tatgaaagaa gttgatgaat
     7861 tggcagaact ggacatcgag gtgattgctc tggattgtac caagcgtgaa cgctacgatg
     7921 gtttggaaat tcaagagttc attcgtcagg ttaaggagaa atatcctaat cagcttttga
     7981 tggctgatac tagtatcttc gaagaagggc tagcagctgt agaagcagga attgactttg
     8041 tcggaacaac cttatcaggc tacacatcct acagtccaaa agtagacggt ccagattttg
     8101 aattgattaa gaaactctgt gatgccggtg tagatgtcat tgcagaagga aaaattcata
     8161 caccagaaca agccaaacaa atccttgaat atggagtgcg aggcatcgtt gttggtggcg
     8221 ccattactag accaaaagag attacagaac gcttcgttgc tagtcttaaa taagatgtga
     8281 gggggagttt tatgtttaaa gttttacaaa aagttggaaa agcttttatg ttacctatag
     8341 ctatacttcc tgcagcaggt ctacttttgg ggattggtgg tgcactttca aacccaacca
     8401 cgatagcaac ttatccaata ctagacaata gtatttttca atcaatattc caagtaatga
     8461 gctctgcagg agaggttgta ttcagtaatt tgtcactact tctctgtgtg ggattatgta
     8521 ttggcttagc gaaacgagat aaaggaaccg ctgcgttagc aggagtaact ggttacttag
     8581 ttatgactgc aacgatcaaa gctttagtaa aactttttat ggcagaagga tctgcaattg
     8641 atactggagt tattggagca ttagttgtcg gaatagttgc cgtatatttg cacaaccgat
     8701 ataacaatat tcaattacct tccgctttag gattctttgg aggttcacgc ttcgttccta
     8761 ttgttacatc gttctcttct atcttgattg gctttgtctt ctttgttatt tggccacctt
     8821 tccaacaact tcttgtttct acaggtggat atatttctca ggcgggtcca attggaactt
     8881 ttctatatgg atttttaatg agactttctg gagcagtagg cttacatcat ataatttacc
     8941 ctatgttttg gtatactgaa cttggtggtg ttgaaactgt tgcaggacaa acagtggttg
     9001 gagctcaaaa aatatttttt gctcaattag ccgatttggc ccattctgga ttatttacag
     9061 aaggaacaag gttttttgca ggtcgtttct caacaatgat gttcggttta ccggctgcct
     9121 gtttagcgat gtaccatagt gttcctaaaa atcgtcgtaa aaaatacgcg ggtttgtttt
     9181 tgggagttgc tttaacatct tttattaccg gtattacaga accaattgaa tttatgtttc
     9241 tattcgtcag tccggttcta tatgttgttc acgcattcct tgatggtgtt agcttcttta
     9301 ttgcagacgt cttaaatatt tcaataggaa acacattttc aggaggtgta atcgatttca
     9361 cattatttgg aattttgcag gggaacgcta agacgaattg ggttcttcag attccatttg
     9421 gacttatttg gagtgttttg tattatatta tttttagatg gttcattact caattcaacg
     9481 ttctaacgcc aggacgagga gaagaagtag attctaaaga aatttctgaa tccgcagatt
     9541 cgacttcaaa tactgcagat tatttaaaac aggatagcct acaaattatc agagccttgg
     9601 gtggatcaaa taatatagaa gatgtagatg cttgtgtgac acgtttacgt gtagctgtaa
     9661 aagaagttaa tcaagttgat aaagcacttt taaaacaaat tggtgcagtt gatgtcttag
     9721 aagtgaaggg tggcattcaa gcaatctatg gagcaaaagc aatcttatat aaaaatagta
     9781 ttaatgaaat tttaggtgta gatgattaag tacttactga cttaataaaa aacagaggag
     9841 agtgatggat gagtaggatg aaatgaaatc gcatacaaga aataaagaac tcattatcca
     9901 agttggatac gcttattaca taggagaata caaatgaaat ttagaaaatt agcttgtaca
     9961 gtacttgcgg gtgctgcggt tcttggtctt gctgcttgtg gcaattctgg cggaagtaaa
    10021 gatgctgcca aatcaggtgg tgacggtgcc aaaacagaaa tcacttggtg ggcattccca
    10081 gtatttaccc aagaaaaaac tggtgacggt gttggaactt atgaaaaatc aatcatcgaa
    10141 gcgtttgaaa aagcaaaccc agatataaaa gtgaaattgg aaaccatcga cttcaagtca
    10201 ggtcctgaaa aaatcacaac agccatcgaa gcaggaacag ctccagacgt actctttgat
    10261 gcaccaggac gtatcatcca atacggtaaa aacggtaaat tggctgagtt gaacgacctc
    10321 ttcacagatg aatttgttaa agatgtcaac aatgaaaaca tcgtacaagc aagtaaagct
    10381 ggagacaagg cttatatgta tccgattagt tctgccccat tctacatggc aatgaacaag
    10441 aaaatgttag aagatgctgg agtagcaaac cttgtaaaag aaggttggac aactgatgat
    10501 tttgaaaaag tattgaaagc acttaaagac aagggttaca caccaggttc attgttcagt
    10561 tctggtcaag ggggagacca aggaacacgt gcctttatct ctaaccttta tagcggttct
    10621 gtaacagatg aaaaagttag caaatataca actgatgatc ctaaattcgt caaaggtctt
    10681 gaaaaagcaa ctagctggat taaagacaat ttgatcaata atggttcaca atttgacggt
    10741 ggggcagata tccaaaactt tgccaacggt caaacatctt acacaatcct ttgggcacca
    10801 gctcaaaatg gtatccaagc taaactttta gaagcaagta aggtagaagt ggtagaagtg
    10861 ccattcccat cagacgaagg taagccagct cttgaatacc ttgtaaacgg gtttgcagta
    10921 ttcaacaata aagacgacaa gaaagtcgct gcatctaaga aattcatcca gtttatcgca
    10981 gatgacaagg agtggggacc taaagacgta gttcgtacag gtgctttccc agtccgtact
    11041 tcatttggaa aactttatga agacaaacgc atggaaacaa tcagcggctg gactcaatac
    11101 tactcaccat actacaacac tattgatgga tttgctgaaa tgagaacact ttggttccca
    11161 atgttgcaat ctgtatcaaa tggtgacgaa aaaccagcag atgctttgaa agccttcact
    11221 gaaaaagcaa acgaaacaat caaaaaagct atgaaacaat agtccttagt tattctataa
    11281 aaagtagttt tttaaagaac ctaagagtgt ataccccctt ttccctctac acagatagtg
    11341 taagaaaagg gggcttttgt ttaaaatgta agaaactgtc acgaaattaa aatgaagttc
    11401 ttacataagc gaatcataaa aaatttcatt ttgattttaa aacagttcaa gaaagtcaaa
    11461 aaattattct atttgaaaga gaggtgccga ctgtgaaagt caataaaatc cgtatgcggg
    11521 aaacagtgat ttcctacgct ttcctagcac cagtattatt cttctttgtc atctttgtgt
    11581 tggctccgat ggtgatgggc ttcattacaa gtttctttaa ctactcaatg actaaatttg
    11641 agtttgtagg cttggataac tatatccgta tgtttaaaga tcctgtcttt acaaaatctc
    11701 tgattaacac agttattttg gttattggat ctgtaccagt tgttgttcta ttctcactct
    11761 ttgtagcatc tcagacctat catcaaaatg tcattgccag atccttctac cgtttcgtct
    11821 tcttccttcc tgttgtaacg ggtagtgttg ccgtgacagt tgtttggaaa tggatttatg
    11881 acccactatc agggattcta aactttgtcc ttaagtcaag ccacatcatc agccaaaaca
    11941 tttcttggtt gggagataaa aactgggcat tgatggcgat tatgattatt ctcttgacca
    12001 cttcagttgg tcagcccatc atcctttata tcgctgccat ggggaatatt gacaattcac
    12061 tggttgaagc ggcgcgtgtt gatggtgcaa ctgagtttca agttttttgg aagattaaat
    12121 ggccaagcct tcttccaaca actctttata ttgcaatcat cacaacaatt aactcattcc
    12181 agtgtttcgc cttgattcag cttttgacat ctggtggtcc aaactactca acaagtacct
    12241 tgatgtacta cctttacgaa aaagccttcc aattgacaga atacggctat gccaacacaa
    12301 ttggtgtctt cttggcagtc atgattgcta tcgtaagctt tgttcaattt aaagtacttg
    12361 gaaacgacgt agaatactaa agaaaggaga cagctatgca atctacagaa aaaaaaccat
    12421 taacagcctt tactgttatt tcaacaatca ttttgctctt gttgactgtg ctgttcatct
    12481 ttccattcta ctggattttg acaggggcat tcaaatcaca acctgataca attgttattc
    12541 ctcctcagtg gttccctaaa atgccaacca tggaaaactt ccaacaactc atggtgcaga
    12601 accctgcctt gcaatggatg tggaactcag tatttatctc attggtaacc atgttcttag
    12661 tttgtgcaac ctcatctcta gcaggttatg tattggctaa aaaacgtttc tatggtcaac
    12721 gcattctatt tgctatcttt atcgctgcta tggcgcttcc aaaacaagtt gtccttgtac
    12781 cattggtacg tatcgtcaac ttcatgggaa ttcatgatac tctctgggca gttatcttgc
    12841 ctttgattgg atggccattc ggtgtcttcc tcatgaaaca gttcagtgaa aatatcccta
    12901 cagagttgct tgaatcagct aaaatcgacg gttgtggtga gattcgtacc ttctggagtg
    12961 tagccttccc gattgtgaaa ccagggtttg cagcccttgc aatctttacc ttcatcaata
    13021 cttggaatga ctacttcatg caattggtaa tgttgacttc acgtaacaat ttgaccatct
    13081 cacttggggt tgcgaccatg caggctgaaa tggcaaccaa ctatggtttg attatggcag
    13141 gagctgccct tgctgctgtt ccaatcgtca cagtcttcct agtcttccaa aaatccttca
    13201 cacagggtat tactatggga gcggtcaaag gataatactc tgcgaaaatc gaatgcaaac
    13261 tacgtcagct tcaccttgcc atacttaagt attgcctgcg gttagcttcc tagtttgttc
    13321 ttcaattttc attgagtata ggaaaatcaa tctatcaaga tacagaagta tattttatag
    13381 atttagagaa tatagaggtt ataagtgtct acaaaatgga gggtatgcag ttactttatg
    13441 aagttttgtc agacacttat caacttaaga atggttttag ttaactatca gaaacgaagg
    13501 aaagagtatg atttttgacg atttgaaaaa catcaccttt tacaaaggga ttcatcccaa
    13561 tttagacaag gctatcgact acctctacca acatcgtaaa gattcattcg aattaggaaa
    13621 gtatgagatt gatggagata aagtctttct agttgttcag gaaaatgtcc tcaatcaagt
    13681 tgagaataat caatttgaac accataagaa ctatgcagat ttgcatttgc tgatagaagg
    13741 gcatgaatat tcgagctacg gttcacgtat caaagacgag gcagtagcat tcgacgaagc
    13801 gagtgacatt ggctttgttc attgtcatga acactaccca ctcttattgg gttatcacaa
    13861 ttttgcgatt ttcttcccag gtgagccaca tcagccaaat ggttatgcag gcatggaaga
    13921 aaaggttcga aaatatctct ttaaaatttt gattgattaa aaataggatg aattgttttt
    13981 ttgtaaagct ttgacaatac tctaccatga aattgatctt tgtgaggtag agaaatgaga
    14041 ataaaatatt taaaaattgg tatcttctaa gtatgctgca agagccagtt tcttagatgg
    14101 acaggggatt acagttgatg agatgtcctg gataattaat ggcattgtga atggattgat
    14161 tggtagatac ataaaattag gtatttatgc agccaagtat ggtattagta tggtgcgatc
    14221 gatcttaagt agggcagctg caactgcggc agcaagagta ggattattga ccaagatttt
    14281 tggatggatt ttatgaatag ctgtgctcgc agctgatgta tatggtaatt ttgccaacaa
    14341 tattgctgca gcttgggatg catatgataa aattcctaac aatggtcgta taaactttta
    14401 aaatgcgaga atgaaagcac tttgtatttt tttattgaat atgttagctt ggacagtgct
    14461 tgcaatgata attcgtggag ggctagatgg atttgatagg catacttgga gtactatttt
    14521 aattgcttcg ctgtccggga tatatgatta taagcccata gataaaaata gaaaaaagtc
    14581 caaaagaaaa aatagatttg ttcatggtag ggacttatga aagctttact gacaaaaaag
    14641 aaaacagttt acaaagaaaa atgatggagg agcaaacatg gcacaaaaag gagtaagcct
    14701 tatcaaggca gcatttgata cagataactt tctcatgcgt tttagtgaga aggtcttgga
    14761 catcgtgaca gccaatcttc tttttgtcgt ctcttgttta cccatcgtga cgattggagt
    14821 ggctaaaatc agcctctacg agaccatgtt cgaagttaag aagagcagac gggtgcctgt
    14881 ttttaaaatc tatctaagat ctttcaagca aaatctgaaa ctaggtcttc agctgggttt
    14941 aatggagtta ggaattgtgt ttcttaccct ttcagatctc tatcttttct ggggtcaaac
    15001 agctctgccc ttccaattgc tgaaagccat ttgtttaggt attctgattt ttcttactat
    15061 cgtgatgctg gctagttacc ctatcgcggc acgttatgac ctatcttgga aagaaattct
    15121 tcaaaaagga ttgatgttgg ctagttttaa ctttccttgg ttcttcctca tgttagccat
    15181 tcttgtcctc attgtgatgg ttctttatct gtccgccttc agtctactct taggtggctc
    15241 agccttccta ctttttgggt ttggactatt ggtctttatc cagactggat tgatggagaa
    15301 aattttcgca aaataccaat aggagcttta tttctgaaac tactttcaaa ggctccaaac
    15361 gctattctat aagcgagaaa ctaaaatcta agtgtctata agatattgaa agccttttcg
    15421 gtaaagtgta tactaaagaa gtaaagaaaa atctgcttta gcagaaaata aaaataatag
    15481 gagaaaatct atgtcagatt tgaaaaaata cgaaggtgtc attccagcct tctacgcatg
    15541 ttatgatgat caaggagaag taagcccaga acgtacgcgt gccttggttc aatacttcat
    15601 tgataaaggt gttcaaggtc tttatgtcaa tggttcttct ggtgaatgta tctaccaaag
    15661 cgttgaagat cgcaagttga ttttggaaga agtcatggcg gtagctaaag gtaaattgac
    15721 cattattgcc catgttgcct gcaacaatac taaagacagt atggaacttg ctcgccatgc
    15781 tgaaagcttg ggagtagatg ctattgcaac gattccacca atttatttcc gcttgccaga
    15841 atactcagtt gccaaatact ggaacgatat cagttctgca gctccaaaca cagactacgt
    15901 gatttacaac attcctcaat tggcaggggt tgctttgact ccaagccttt acacagaaat
    15961 gttgaaaaat cctcgtgtta tcggtgtgaa gaactcttct atgccagttc aagatatcca
    16021 aacctttgtg agccttggtg gggaagacca tatcgtcttt aacggtccag atgaacagtt
    16081 cctaggtggt cgcctcatgg gtgccaaagc tggtatcggt ggtacttatg gtgctatgcc
    16141 agaactcttc ttgaaactca accaattgat tgcggataaa gatttagaaa cagcgcgtga
    16201 gttacagtat gctatcaacg ctatcattgg taaattgact gctacacatg gaaatatgta
    16261 cggtgtcatc aaagaagttt tgaagatcaa tgaaggcttg aatattggtt ctgttcgttc
    16321 accattgaca ccagtgactg aagaagatcg tccagttgta gaagcggctg ctgccttgat
    16381 tcgtgaaacc aaggagagct tcctctaatc taaaaggagg tatttatgac atactacgtt
    16441 gcaattgata tcggtggaac caacatcaag tatggtttgg ttgatcaaga ggggcaactt
    16501 cttgaatcgc atgaaatgcc aactgaggcg cataagggtg gacctcatat cttacaaaag
    16561 accaaagata tcgtagctag ttatttagaa aaaggcccag tagcaggtgt tgccatatct
    16621 tctgctggga tggtggatcc ggataagggt gagattttct atgctgggcc gcaaatccct
    16681 aactacgcag gcacccagtt caaaaaggaa atcgaagaaa gctttactat tccttgtgag
    16741 attgaaaatg atgtcaactg tgcaggtctt gctgaggcag tatctggttc aggcaaggga
    16801 gcaagtgtga cactttgctt gaccattgga acaggtatcg gtggttgctt gattatggat
    16861 aggaaagtct tccatggttt tagcaattca gcctgtgaag tcgggtatat gcatatgcag
    16921 gatggagctt ttcaagactt ggcttctaca acagctttag tgaaatatgt agctgaagcc
    16981 catggagaag atgttgatca gtggaatggc cgtagaattt tcaaagaagc cactgaagga
    17041 aacaaaatct gcatggaagg tattgaccgt atggttgact atctaggaaa aggtctggca
    17101 aatatttgct acgttgccaa tccagaagtg gttattcttg gtggtggtat catggggcaa
    17161 gaggctatcc tcaaacctaa gattcgcaca gccttgaaag aggctttggt accaagttta
    17221 gcagaaaaaa cacgattaga atttgcccat caccaaaata cagcagggat gttgggtgca
    17281 tattatcatt ttaagacaaa acaatcctag tttggctcag ccaaactagg attttcttac
    17341 acgtttttgt ctacgatagc cgttgagttt tttattttcc cagtagctat taaagatttt
    17401 ttccttgctt tcgcgattga tttccaaaaa gtaggcataa atcaaatcga taaagaagag
    17461 cataggaagt tgagcggata ttcgttggat gtaggagggt tggctgtggg tggctacaag
    17521 aacagtctct gtataggtct ggctattttt attgggaaca cttgtaaaga gtacagtctt
    17581 tgcccccatc tccttggcat ctaatagact atctaaaata gaaggagttg agcctgaaag
    17641 tgagaagccc agtactagac aattttcatc catgatgctg gttgtccagg caaagccgtc
    17701 ttggtctgtc aaagcttcgc agaccacacc tagtcgcata aaacgtaatt tcatttcacg
    17761 ggcgacgagg ccagaactcc ctgttccaaa gaagtagata cgctcagcat cttcgattag
    17821 ctgggcaatt cgctctagtt ggatttcgtc aatcaagtct tgtgtttgtt ccctcatatt
    17881 gctataactt ctgaggactc gtttggtcag tggactatgc ttggagactt ggttggcttg
    17941 attttctgcc tgatgttggt attggaaaat aaattctcgg tagccagtaa agccacactt
    18001 tttagcaaag cgggtcaaag cagcttgaga aatatgtaat ttttgggtga cttgttgaga
    18061 agataaatca tctgtaatcg tttcagcttg caaaaaatag cgagcgattt cttgctctag
    18121 gtctgtcatt tcttcaaaat gtgaatcaat gatagttgcg atatctggtt tgtccatagg
    18181 gaaagctcct ttacatgagt catactggaa gactagatca gggaatagtc acacttcatt
    18241 ataacacata atataaggat agataaataa aaacgcatct ctgttttaaa aacgaaaaaa
    18301 tcgaaaaagc ttctctcttt tccataattt tctactcaaa ttgtggtaca attaagagta
    18361 agattttaag ttagaaatga gactgatttg tatgagaaaa tttaacagcc attcgattcc
    18421 gattcggctt aatttattgt tttcaatcgt cattttactc tttatgacca ttattggtcg
    18481 tttgttgtat atgcaggttt tgaacaagga tttttacgaa aaaaagctag cctcagctag
    18541 tcagaccaag attacaagca gttcagcccg tggggaaatt tatgatgcta gtggaaaacc
    18601 tttggtagaa aatacgttaa agcaggttgt ttcctttacg cgtagcaata aaatgacggc
    18661 tacagactta aaagaaacag ctaaaaagtt actgacttat gtgagcatca gttctccaaa
    18721 tttgacagaa cgccagctgg cggattacta tttggctgat cctgaaatct ataaaaaaac
    18781 agtggaagct ctcccaagcg agaaacgctt ggattcagat ggcaatcgtc tatccgaatc
    18841 agaactgtat aacaatgcgg tcgatagtgt acaaacgagt caactaaact atacagagga
    18901 tgaaaagaaa gaaatctatc tttttagtca gttaaatgct gttggaaact ttgcgacagg
    18961 aaccattgct acagatcctc taaatgattc tcaggtggct gttattgcct ctatttcaaa
    19021 ggagatgcct ggcattagta tttctacttc ttgggataga aaggttttgg aaacttccct
    19081 ttcttctata gttgggagtg tatccagtga aaaagctggt ctcccagcgg aagaagcaga
    19141 agcctatctt aaaaaaggct attctctaaa tgatcgtgta ggaacctcct atttggaaaa
    19201 gcaatatgaa gagaccttac aaggaaaacg ctcggtaaaa gaaatccatc tggataaata
    19261 tggcaatatg gaaagcgtgg atacaattga ggaaggtagt aagggaaaca atatcaaact
    19321 gaccattgat ttggccttcc aagatagcgt ggatgctttg ctgaaaagtt atttcaattc
    19381 tgagctagaa aatggtggag ccaagtattc tgaaggtgtc tatgcagtcg cccttaaccc
    19441 aaaaacaggt gcggttttgt ctatgtcagg gattaaacat gacttgaaaa cgggagagtt
    19501 gacgcctgat tccttgggaa cggtaaccaa tgtctttgtt ccaggttcgg ttgtcaaggc
    19561 ggcgaccatc agctcaggtt gggaaaatgg agtcttgtca ggaaaccaga ccttgacaga
    19621 ccagtccatt gtcttccaag gttcagctcc catcaattct tggtatactc aggcttacgg
    19681 ttcattccct atcacagcgg tccaagctct ggagtattca tcaaatacct atatggtcca
    19741 aacagcctta ggtcttatgg ggcaaaccta tcaacccaat atgtttgtcg gcaccagcaa
    19801 tctagagtct gctatggaga aactgcgttc aacctttggc gaatatggct tgggtactgc
    19861 gacaggaatt gacctaccag atgaatctac tggatttgtt cccaaagagt atagctttgc
    19921 taattacatt actaatgcct ttgggcagtt tgataactat acgccgatgc agttggctca
    19981 gtatgtagca actattgcaa ataatggtgt tcgtgtggct cctcgtattg ttgaaggcat
    20041 ttatggtaat aatgataagg gaggactggg tgacttgatt cagcaactgc aaccgacaga
    20101 gatgaataag gtcaatatat ccgactccga tatgagcatc ttgcaccaag gtttttatca
    20161 ggttgcccat ggtactagtg gattgacaac tggacgtgcc ttttcaaatg gtgccttggt
    20221 atccattagc ggaaaaacag gtacagccga aagctatgtg gcagatggtc agcaagcaac
    20281 caataccaat gcggtggcct atgccccatc tgataatccc caaatcgctg tcgcagtggt
    20341 ctttcctcat aataccaatc taacaaatgg tgtaggacct tccattgcgc gtgacattat
    20401 caatctgtat caaaaatacc atccaatgaa ctagaaagga aattatgctt tatccaacac
    20461 ctattgccaa gttgattgac agttattcta agttaccggg tatcgggatt aagacggcta
    20521 cgcgtctggc cttttatacg attgggatgt ctgctgatga tgtcaatgaa tttgcaaaaa
    20581 atctcctttc tgctaagaga gaattgacat attgttctat ttgtggacgt ttgacagacg
    20641 acgatccttg ttctatctgt actgatccga ctcgtgacca gacaacaatt ttagttcttg
    20701 aggatagtag agatgtggca gccatggaaa atatccaaga ataccatgga ctctatcatg
    20761 tccttcatgg cctcatttct cctatgaatg gtatcagtcc ggacgatatc aatctcaaga
    20821 gccttatgac tcgtcttatg gatagtgagg tttcagaagt gattgtggcg actaatgcta
    20881 cagcggatgg tgaagcgact tccatgtatc tttcacgttt gctcaagccg gctggtatca
    20941 aggttacgcg tctagcacga ggtctcgctg tgggagcgga cattgagtat gcggacgaag
    21001 tgacactctt acgagccatt gaaaatcgga cagagttgta agtgtaggca aatttacgaa
    21061 ctccattcat ttataaaaaa tcaaagaggc tgaaaatcgt tcctatcggc ctctttttgt
    21121 atagtgtgat gagtaggctc aggttcaagt tttaaaaaac caagcaaata tgatatacta
    21181 aagagcgagt attctagtag aattaggaca aataatatga aacaaacgat tattctttta
    21241 tatggtggac ggagtgcgga acgcgaagtc tctgtccttt cagctgagag tgtcatgcgt
    21301 gcggtcaatt acgaccgttt cacagtcaag actttcttta tcagtcagtc aggtgacttt
    21361 atcaaaacgc aggaatttag tcatgctccg gggcaagaag accgtctcat gaccaatgaa
    21421 accattgatt gggataagaa agttgcacca agtgctatct acgaagaagg tgcagtggtc
    21481 tttccagtcc ttcacgggcc aatgggagaa gatggctctg ttcaaggatt cttggaagtt
    21541 ttgaaaatgc cttacgttgg ttgcaacatt ttgtcatcaa gtcttgccat ggataaaatc
    21601 acgactaagc gtgttctgga atctgctggt attgcccaag ttccttatgt ggctatcgtt
    21661 gaaggcgatg atgtgactgc taaaatcgct gaagtggaag aaaaattggt ttatccagtc
    21721 ttcactaagc cgtcaaacat ggggtctagt gtcggtattt ctaagtctga aaaccaagaa
    21781 gaactccgtc aagccttaaa acttgccttc cgatatgaca gccgtgtctt ggttgagcaa
    21841 ggagtgaatg cccgtgaaat tgaggttggc ctcttgggta actacgatgt caagagcacg
    21901 ctaccaggag aagttgtcaa ggacgttgcc ttttatgact acgatgccaa gtatattgat
    21961 aacaagatta ctatggatat tcctgccaaa atcagtgatg atgtggtggc tgtcatgcgt
    22021 caaaatgcag aaacagcctt ccgtgccatt ggtggccttg gtctatctcg ttgcgatttc
    22081 ttctatacag ataagggaga gatttttctc aacgagctca atactatgcc aggtttcacc
    22141 cagtggtcta tgtacccact actttgggac aatatgggga tcagctaccc aaaactaatc
    22201 gagcgtttgg ttgaccttgc caaggaaagt tttgacaagc gcgaagcgca tttgatataa
    22261 aatgaaagag agggtagaag ccagaaccat cactgcaagg tgactagagt tctcggactt
    22321 cagccctttt taaaggagta gaaatgaaat taacaatcca tgaaattgcc caagttgttg
    22381 gagtcaaaaa tgatatcagt atctttgagg acacccagtt agaaaaagct gagtttgata
    22441 gtcgtttgat tggaactgga gatttatttg tgccacttaa aggtgcgcgt gatggccatg
    22501 actttattga aacagccttt gaaaatggtg cagcagtaac cttgtctgag aaagaggtct
    22561 caaatcatcc ttacattcta gtagatgatg ttttgacagc ctttcaatcc ctagcatcct
    22621 actatcttga aaaaacgact gttgatgtct ttgctgttac aggttcaaat ggcaagacaa
    22681 cgactaagga tatgttggcg catttactgt caacaagata caagacctac aaaacacaag
    22741 gcaattacaa taatgagatt ggccttcctt acacagttct tcatatgcct gaaggaacag
    22801 aaaagttggt tttggagatg ggacaggatc acttgggaga tattcatctc ttgtctgaat
    22861 tggctcgtcc aaaaacagcc atcgtgacct tggttggaga agctcatttg gcctttttca
    22921 aagatcgttc tgagattgct aaaggaaaaa tgcaaattgc agacggaatg gcttcaggtt
    22981 ccttgctttt aatgccggct gaccctatcg tagaggacta tttgccaact gataaaaagg
    23041 tggtccgttt tgggcaaggg gcagagttgg aaatcacaga cttggttgag cgtaaggaca
    23101 gtctgacctt taaggctaat ttcttggaac aagcccttga tttgccagtg actggtaagt
    23161 acaatgctac caatgctatg attgcatcct atgttgccct acaagaagga gtttcagagg
    23221 agcaaattca tcaggccttc caaggtcttg aattgacgcg taatcgtact gagtggaaga
    23281 aagcagccaa tggagcagat atcctgtcag acgtatataa tgccaatcca actgctatga
    23341 agctgatttt agagactttc tctgccattc cagccaatga aggtggcaag aaaattgcag
    23401 tgttggcgga tatgaaggag cttggtgacc agtctattca actccataac cagatgattt
    23461 tgagcctttc tccagatgtg cttgataccg ttattttcta tggagaagac attgctgaat
    23521 tagcccaatt ggccagtcaa atgttcccaa ttggccacgt ttactacttc aagaaaacag
    23581 aagaccaaga ccaatttgaa gacctagtca agcaggtcaa agaaagcctt ggggctcatg
    23641 accaaatcct gctcaaaggc tctaactcta tgaatctagc caagttggta gaaagtttag
    23701 aaaatgaaga caagtgattt tgtcaagtat ttgcaaagaa tgattgccat tacagatact
    23761 ggcttaacct ttacaaaaga tccttttgac cgtgagcgct acgaagactt gcgagacctt
    23821 ttatctgaaa tgttgaatca agcatcagac cttgattccg aagaagtggc agaagtcttg
    23881 aagccaactt ctgcttatgc gactccctta atggacgtcc gtgcttggat tgttgaggac
    23941 gaaaagattt gtctggttag gggacaagga gaggatagtt gggctttgcc aggtggcttt
    24001 ggtgaagtcg gctattctcc aactggaaat attctcaagg aaattgaaga agaaaccggt
    24061 tttaaagcca aagttgaaag actgctagct gtttttgata ccaatcgttt ccaactacag
    24121 agcaaacaat atgcaaagtt tgtttttgaa tgtaagcttc tcgatggaca attccaagaa
    24181 aatcaagaaa tcgctgacct tcaatttttt gcaattgacc aactgccgaa tttatctgaa
    24241 aaacgcatta ccaaggagca aatcgagatt ctttggcagg tttatcaagg tcagagggag
    24301 caatatcttg actaagaaga tgattatcgt atttctaaat ccatttttga caactagcat
    24361 ggtataataa tatgcaggaa aattttgaat tatgaggaag actagatgaa tttatgggat
    24421 attttcttta cgactcaggc aaccgagccg cccaaatttg accttttttg gtatgttagc
    24481 ctatttacgc tcttagcctt aaccttttat acagcccatc gctatcgtga aaagaaggtt
    24541 taccaacgat ttttccaaat cttgcagact gttcagttaa tccttcttta tggttggtac
    24601 tgggttaatc atatgccact gtcagaaagc ctaccctttt accattgccg tatggctatg
    24661 tttgtggtac tcttgcttcc tggtcagtcc aaatataaac aatactttgc attattggga
    24721 acatttggga cattagcagc ctttgtttat ccagtgccag atgcttaccc ttttccacat
    24781 attaccattt tatcctttat ctttggtcat ttagcactct tggggaactc tctagtttat
    24841 ctattgagac agtataatgc gcgattgctg gatgtgaagg gaatttttct catgaccttt
    24901 gccctaaatg ccttgatttt tgtggtcaat ttggtgacag gtggcgatta cggatttttg
    24961 acaaaaccgc cattggttgg ggatcacggt ctagtagcta attatttact tgtttcaatt
    25021 gtgctggtag ctactatcag tttgactaag aaaatcttag aattcttttt agctcaagaa
    25081 gcagaaaaaa tgattgcaaa ggaagcttaa cacagagctt tcttttttgc tcttagagag
    25141 tttttacaag cagcttataa aataagaatt tctgaataga caaactcaaa aaatggctgg
    25201 gaaatttagg aaaaaagcaa gcacgattaa attttttgtg ttataatatt ttgtgaatag
    25261 ctatgcctat gtttagctat ggaataatac gaagtgcgaa acttggaaga tagagaggaa
    25321 gcgatgtaat ggctagagaa ggctttttta caggtctaga tattggaaca agctctgtca
    25381 aggtgcttgt ggccgagcag agaaatggtg aattaaatgt aattggcgtg agtaatgcca
    25441 aaagtaaagg tgtaaaggat ggaattattg ttgatattga tgcagcagca actgctatca
    25501 agtcagccat ttcccaagcg gaagaaaagg caggcatttc gattaaatca gtgaatgtcg
    25561 gcttgcctgg taatcttttg caggtagaac caactcaggg gatgattcca gtaacatctg
    25621 atactaagga aattacggat caagatgttg aaaatgttgt caaatcagct ttgacaaaga
    25681 gtatgacacc tgaccgtgaa gtcattacct ttattcctga agaatttatt gtggatggtt
    25741 tccaagggat tcgtgaccca cgtggcatga tgggggttcg ccttgaaatg cgtggtttgc
    25801 tttatacagg acctcgtact atcttgcaca atttgcgtaa gacggttgag cgtgcaggtg
    25861 ttcaggttga aaatgttatc atttcaccac tagcaatggt tcagtctgtt ttgaacgaag
    25921 gggaacgtga atttggtgct acagtgattg atatgggggc aggtcaaacg actgtcgcta
    25981 caatccgtaa tcaagaactc cagttcacac atattctcca agaaggtgga gattatgtaa
    26041 ctaaagatat ctccaaggtt ttgaaaacct ctcgcaaatt agcggaaggc ttaaaactga
    26101 attacgggga agcctatccg cctcttgcaa gcaaagaaac cttccaagta gaggttattg
    26161 gagaagtaga agcagtcgaa gtgacggaag cctacttgtc agaaattatt tctgcacgaa
    26221 tcaagcacat ccttgaacaa atcaagcaag aattagatag aaggcgtcta ttggacctcc
    26281 ctggtggtat tgtcttaatc ggtgggaatg ccattttacc aggtatggtt gagcttgctc
    26341 aggaagtctt tggcgtccgt gtcaagcttt atgttccaaa tcaagttggt atccgtaatc
    26401 cagcctttgc gcatgtgatt agtttatcag aatttgcggg tcaattaaca gaagttaatc
    26461 ttttggctca gggagcgata aaaggtgaga atgacttaag tcatcagcca attagttttg
    26521 gtgggatgct gcaaaaaaca gctcagtttg tacaatcaac gcctgttcaa ccagctcctg
    26581 ctccagaagt agagccggtg gcgcctacag aaccaatggc ggatttccaa caagcttcac
    26641 aaaataaacc gaaattagca gatcgtttcc gtggcttgat cggaagcatg tttgacgaat
    26701 aaagaggaaa aataaattat gacattttca tttgatacag ctgctgctca aggggcagtg
    26761 attaaagtaa ttggtgtcgg tggaggtggt ggcaatgcca tcaaccgtat ggtcgacgaa
    26821 ggtgttacag gcgtagaatt tatcgcagca aacacagatg tacaagcatt gagtagtaca
    26881 aaagctgaga ctgttattca gttgggacct aaattgactc gtggtttggg tgcaggaggt
    26941 caacctgagg ttggtcgtaa agccgctgaa gaaagcgaag aaacactgac ggaagctatt
    27001 agtggtgccg atatggtctt catcactgct ggtatgggag gaggctctgg aactggagct
    27061 gctcctgtta ttgctcgtat cgccaaagat ttaggtgcgc ttacagttgg tgttgtaaca
    27121 cgtccctttg gttttgaagg aagtaagcgt ggacaatttg ctgtagaagg aatcaatcaa
    27181 cttcgtgagc atgtagacac tctattgatt atctcaaaca acaatttgct tgaaattgtt
    27241 gataagaaaa caccgctttt ggaggctctt agcgaagcgg ataacgttct tcgtcaaggt
    27301 gttcaaggga ttaccgattt gattaccaat ccaggattga ttaaccttga ctttgccgat
    27361 gtgaaaacgg taatggcaaa caaagggaat gctcttatgg gtattggtat cggtagtgga
    27421 gaagaacgtg tggtagaagc ggcacgtaag gcaatctatt caccacttct tgaaacaact
    27481 attgacggtg ctgaggatgt tatcgtcaac gttactggtg gtcttgactt aaccttgatt
    27541 gaggcagaag aggcttcaca aattgtgaac caggcagcag gtcaaggagt gaacatctgg
    27601 ctcggtactt caattgatga aagtatgcgt gatgaaattc gtgtaacagt tgtcgcaacg
    27661 ggtgttcgtc aagaccgcgt agaaaaggtt gtggctccac aagctagatc tgctactaac
    27721 taccgtgaga cagtgaaacc agctcattca catggctttg atcgtcattt tgatatggca
    27781 gaaacagttg aattgccaaa acaaaatcca cgtcgtttgg aaccaactca ggcatctgct
    27841 tttggtgatt gggatcttcg ccgtgaatcg attgttcgta caacagattc agtcgtttct
    27901 ccagtcgagc gctttgaagc cccaatttca caagatgaag atgaattgga tacacctcca
    27961 tttttcaaaa atcgttaagt aaatgaatgt aaaagaaaat acagaacttg tttttcgaga
    28021 agttgcagag gctagtctga gtgctcatcg agagagtggt tcggtctctg tcattgcagt
    28081 taccaagtat gtagatgtac cgacagcgga agccttgctt ccgctaggtg ttcatcatat
    28141 cggtgaaaat cgtgtagata agtttctgga aaaatatgaa gctttaaaag atcgagatgt
    28201 gacttggcat ttgattggta ccttgcaaag acgtaaggtg aaagatgtca ttcaatacgt
    28261 tgattatttc catgcattgg actcagtaaa gctagcaggg gaaattcaaa aaagaagtga
    28321 ccgagtcatc aagtgtttcc ttcaagtaaa tatttctaaa gaagaaagca aacacggttt
    28381 ttcgagagag gaactgctgg aaatcttgcc agagttagcc agactagata agattgaata
    28441 tgttggttta atgacgatgg caccttttga ggctagcagt gagcagttga aagagatttt
    28501 caaggcggcc caagatttac aaagagaaat tcaagagaaa caaattccaa atatgcctat
    28561 gaccgagtta agtatgggaa tgagtcgtga ttataaagaa gcgattcaat tcggttccac
    28621 ttttgttcgt ataggtacat cattttttaa gtaggagaga accatgtctt taaaagatag
    28681 attcgataga tttatagatt attttacgga ggatgaggat tcaagtctcc cttatgaaaa
    28741 aagagatgag cctgtgttta ctccagtaaa ttcttcacag gaaccggctc tcccaatgaa
    28801 tcaaccttca cagtcggctg gtacaaaaga gaacaatatc accagacttc atgcaagaca
    28861 acaggaattg gcaaatcaga gtcagcgtgc aacggataag gtcattatag atgttcgtta
    28921 tcctagaaaa tatgaggatg caacagaaat tgttgattta ttggcaggaa acgaaagtat
    28981 cttgattgat tttcagtata tgacagaggt gcaggctcgt cgttgtttgg actatttgga
    29041 tggagcttgt catgttttag ctggaaattt gaaaaaggta gcttctacca tgtatttgtt
    29101 gacaccagtg aatgttattg taaatgttga agatatccgt ttaccagatg aagatcaaca
    29161 gggtgagttc ggttttgata tgaagcgaaa tagagtacga taatgatttt tttaattcgt
    29221 atgatttata atgcagtgga tatttactcc ctgattttgg tagccttcgc tgtcatgtct
    29281 tggtttccag gtgcctacga atccagttta ggtcgttgga ttgtagcgtt ggtgaaacca
    29341 gtgcttgctc ccttgcaacg cctgccttta cagatagcgg gtcttgattt atctgtttgg
    29401 gtcgcgattg ttttggttcg atttttagga gaaaacctag tgcgttttct ggcgatgata
    29461 ggatgaataa agggatttat cagcatttct ccatagaaga tcgtccattt cttgacaagg
    29521 gaatggaatg gataaagaag gtagaagata gctatgctcc ttttttaact ccttttatca
    29581 atcctcatca ggagaagcta ttaaagattt tggccaaaac ctatggtctt gcttgtagca
    29641 gtagtgggga attcgtctcg agtgagtatg ttcgagtttt attataccca gattatttcc
    29701 aaccagagtt ttcagatttt gaaatatctc tccaggaaat tgtgtattcc aataaatttg
    29761 aatatttaac gcatgctaag attttaggga cagtcatcaa tcaattaggg attgaacgga
    29821 aactttttgg agatatccta gtagatgaag aacgggcgca gattatgatt aatcagcagt
    29881 ttcttcttct ctttcaagat ggactaaaga aaattggtcg tatacctgtt tcgctggagg
    29941 aacgtccttt caccgagaaa atagataagc tagaacagta tcgagaactg gatttatctg
    30001 tgtctagttt tcgattagat gttcttttat caaatgtttt gaaactatct aggaatcaag
    30061 caaatcagtt gattgaaaag aaacttgtcc aagtaaatta tcatgtggta gacaaatcag
    30121 attacactgt tcaagttgga gacttgatta gtgtgagaaa atttggtcgc ttgagattac
    30181 ttcaagataa gggacaaacg aaaaaagaga agaaaaaaat aaccgtccag ttattattaa
    30241 gtaagtgagg aatagaatgc caattacatc attagaaata aaggacaaga cttttggaac
    30301 tcgattcaga ggttttgatc cagaagaagt cgatgaattt ttagatattg tggttcgtga
    30361 ttacgaagat cttgtgcgtg cgaatcatga taaaaatttg cgtattaaga gtttagaaga
    30421 gcgtttgtct tactttgatg aaataaaaga ttcattgagc cagtctgtat tgattgctca
    30481 ggatacagct gagagagtga aacaggcggc gcatgaacgt tcaaacaata tcattcatca
    30541 agcagagcaa gatgcgcaac gcttgttgga agaagctaaa tataaggcaa acgagattct
    30601 tcgtcaagca actgataatg ctaagaaagt cgctgttgaa acagaagaat tgaagaacaa
    30661 gagccgtgtc ttccaccaac gtctcaaatc tacaattgag agtcagttgg ctattgttga
    30721 atcttcagat tgggaagata ttctccgtcc aacagctact tatcttcaaa ccagtgatga
    30781 agcctttaaa gaagtggtta gcgaagtact tggagaaccg attccagctc caattgaaga
    30841 agaaccaatt gatatgacac gtcagttctc tcaagcagaa atggaagaat tacaagctcg
    30901 tattgaggta gccgataaag aattgtctga atttgaagct cagattaaac aggaagtgga
    30961 aactccaact cctgtagtga gtcctcaagt tgaagaagag cctctgctca tccagttggc
    31021 ccaatgtatg aagaaccaga agtagctcca gtgcatccga caggtccaac accagctaca
    31081 gaaactgttg attcagcacc gggatttgaa gcaccgcaag aatctgttac aattttataa
    31141 gaaatattct gagaacaata tcttatcctt atattcccag cgagcaggag atggtgtgag
    31201 tcctgcattc cctatcgata agattatcct ctcaaactat caagtctgaa tctagtaaga
    31261 tttgacgttc cccacgttac gggataagag ggagaaagac taaaactttt tccgaataaa
    31321 ggtggtacca cgattttcgt cctttttgga agtcgtggtt tttaatttgt tattatttat
    31381 aaaggagata ccatgaaact caaagacacc cttaatcttg ggaaaactga attcccaatg
    31441 cgtgcaggcc ttcctaccaa agagccagtt tggcaaaagg aatgggaaga tgcaaaactt
    31501 tatcaacgtc gtcaagaatt gaaccaagga aaacctcatt tcaccttgca tgatggccct
    31561 ccatacgcta acggaaatat ccacgttgga catgctatga acaagatttc aaaagatatc
    31621 attgttcgtt ctaagtctat gtcaggattt tacgcaccat ttattcctgg ttgggatact
    31681 catggtctgc caatcgagca agtcttgtca aaacaaggtg tcaaacgtaa agaaatggac
    31741 ttggttgagt acttgaaact ttgccgtgag tacgctcttt ctcaagtaga taaacaacgt
    31801 gaagatttta aacgtttggg tgtttctggt gactgggaaa atccatatgt gaccttgact
    31861 cctgactatg aagcagctca aattcgtgta tttggtgaga tggctaataa gggttatatc
    31921 taccgtggtg ctaagccagt ttactggtca tggtcatctg agtcagccct tgctgaagca
    31981 gagattgaat accatgactt ggtttcaact tccctttact atgccaacaa ggtaaaagat
    32041 ggcaaaggag ttctagatac agatacttat atcgttgtct ggacaacgac tccatttact
    32101 atcacagcct ctcgtggttt gacggttggt gcagatattg attacgtttt ggttcaacct
    32161 gctggtgaag ctcgtaagtt tgtcgttgct gctgaattat tgactagctt gtctgagaaa
    32221 tttggctggg ctgatgttca agttttggaa acttaccgtg gccaagaact caaccacatc
    32281 gtaacagaac acccatggga tacagctgta gaagagttgg taattcttgg tgaccacgtt
    32341 acgactgact ctggtacagg tattgtccat acagcccctg gttttggtga ggacgattac
    32401 aatgttggta ttgctaataa tcttgaagtc gcagtgactg ttgatgaacg tggtatcatg
    32461 atgaagaatg ctggtcctga atttgaaggt caattctatg aaaaggtagt tccaactgtt
    32521 attgaaaaac ttggtaacct ccttcttgcc caagaagaaa tctctcactc atatccattt
    32581 gactggcgta ctaagaaacc aatcatctgg cgtgcagttc cacaatggtt tgcctcagtt
    32641 tctaaattcc gtcaagaaat cttggacgaa attgaaaaag tgaaattcca ctcagaatgg
    32701 ggtaaagtcc gtctttacaa tatgatccgt gaccgtggtg actgggttat ctctcgtcaa
    32761 cgtgcttggg gtgttccact tcctatcttc tacgctgaag atggtacagc tatcatggta
    32821 gctgaaacta ttgaacacgt agctcaactt tttgaagaac atggttcaag catttggtgg
    32881 gaacgtgatg ccaaagacct cttgccagaa ggatttactc atccaggttc accaaacggc
    32941 gagttcaaaa aagaaactga tatcatggac gtttggtttg actcaggttc atcatggaat
    33001 ggagtggtgg taaaccgtcc tgaattgact tacccagccg acctttacct agaaggttct
    33061 gaccaatacc gtggttggtt taactcatca cttatcacat ctgttgccaa ccatggcgta
    33121 gcaccttaca aacaaatctt gtcacaaggt tttgcccttg atggtaaagg tgagaagatg
    33181 tctaaatctc ttggaaatac cattgctcca agcgatgttg aaaaacaatt cggtgctgaa
    33241 atcttgcgtc tctgggtaac aagtgttgac tcaagcaatg acgtgcgtat ctctatggat
    33301 atcttgagcc aagtttctga aacttaccgt aagattcgta acactcttcg tttcttgatt
    33361 gccaatacat ctgactttaa cccagctcaa gatacagtcg cttacgatga gcttcgttca
    33421 gttgataagt acatgacgat tcgctttaac cagcttgtca agaccattcg tgatgcttac
    33481 gcaaactttg aattcttgac aatctacaag gccttggtga actttatcaa cgttgacttg
    33541 tcagccttct accttgattt tgccaaagat gttgtttaca ttgaaggtgc caaatcactg
    33601 gaacgccgtc aaatgcagac tgtcttctat gacattcttg tcaaaatcac caaactcttg
    33661 actccaatcc ttcctcacac tgcggaagaa atctggtcat atcttgagtt tgaagccgaa
    33721 gacttcgtcc aattgtcaga attaccagaa gttcaaactt ttgctaacca agaagaaatc
    33781 ttggatacat gggcagcctt catggacttc cgtggacaag cacaaaaagc cttggaagaa
    33841 gctcgtaatg caaaagttat cggtaaatca cttgaagcac acttgacagt ttatccaaat
    33901 gaagttgtga aaactctact cgaagcagta aacagcaatg tagctcaact tttgatcgtg
    33961 tctgagttga ccatcgtaga aggaccagct ccggaagcta cccttagctt cgaagatgta
    34021 gtcttcacag ttgaacgcgc tgcaggtgaa gtatgtgacc gttgccgtcg tattgaccca
    34081 acaacagcag aacgtagcta ccacgcagtc atctgtgacc actgtgcaag tatcgtagaa
    34141 gaaaactttg cagatgcagt cgcagaagga tttgaagaga aataagattg aaaagtctag
    34201 gtaaaactca atttgagaag aaaagacaac taattttata gtctattaaa cgcattgtat
    34261 cacgtttttg aacacctgat atgatgcgtt ttttatttat tttaaaaatt tgcgaggtat
    34321 gactttttat actcaacaag aatcaaagag aaacttagca agctaacagt agtaatataa
    34381 aataggaatt tgatatt
//
LOCUS       DAVLRT010000014        33146 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLRT010000014 DAVLRT010000000
VERSION     DAVLRT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.014"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(421..2148)
                     /gene="ezrA"
                     /locus_tag="VXM85_000671"
     CDS             complement(421..2148)
                     /gene="ezrA"
                     /locus_tag="VXM85_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1345295.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAESYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2230..4176)
                     /gene="gyrB"
                     /locus_tag="VXM85_000672"
     CDS             complement(2230..4176)
                     /gene="gyrB"
                     /locus_tag="VXM85_000672"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1345296.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4191..4754)
                     /locus_tag="VXM85_000673"
     CDS             complement(4191..4754)
                     /locus_tag="VXM85_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1345297.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTSFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4839..5393)
                     /locus_tag="VXM85_000674"
     CDS             complement(4839..5393)
                     /locus_tag="VXM85_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1345298.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5404..6627)
                     /locus_tag="VXM85_000675"
     CDS             complement(5404..6627)
                     /locus_tag="VXM85_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1345299.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPRLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6664..9114)
                     /locus_tag="VXM85_000676"
     CDS             complement(6664..9114)
                     /locus_tag="VXM85_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1345300.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSCRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVVSLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9314..10243)
                     /locus_tag="VXM85_000677"
     CDS             complement(9314..10243)
                     /locus_tag="VXM85_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1345301.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10383..10670
                     /locus_tag="VXM85_000678"
     CDS             10383..10670
                     /locus_tag="VXM85_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1345302.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10704..12038)
                     /gene="ciaH"
                     /locus_tag="VXM85_000679"
     CDS             complement(10704..12038)
                     /gene="ciaH"
                     /locus_tag="VXM85_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1345303.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12028..12702)
                     /gene="ciaR"
                     /locus_tag="VXM85_000680"
     CDS             complement(12028..12702)
                     /gene="ciaR"
                     /locus_tag="VXM85_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1345304.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12811..15357)
                     /locus_tag="VXM85_000681"
     CDS             complement(12811..15357)
                     /locus_tag="VXM85_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1345305.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15480..15920)
                     /locus_tag="VXM85_000682"
     CDS             complement(15480..15920)
                     /locus_tag="VXM85_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1345306.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15895..16764)
                     /locus_tag="VXM85_000683"
     CDS             complement(15895..16764)
                     /locus_tag="VXM85_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1345307.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLDRILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16742..17242)
                     /locus_tag="VXM85_000684"
     CDS             complement(16742..17242)
                     /locus_tag="VXM85_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1345308.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17253..17951)
                     /locus_tag="VXM85_000685"
     CDS             complement(17253..17951)
                     /locus_tag="VXM85_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1345309.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQEIQEIFEINYMTPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17944..18180)
                     /locus_tag="VXM85_000686"
     CDS             complement(17944..18180)
                     /locus_tag="VXM85_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1345310.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(18280..19206)
                     /locus_tag="VXM85_000687"
     CDS             complement(18280..19206)
                     /locus_tag="VXM85_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1345311.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19281..20096)
                     /locus_tag="VXM85_000688"
     CDS             complement(19281..20096)
                     /locus_tag="VXM85_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345312.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20100..20420)
                     /locus_tag="VXM85_000689"
     CDS             complement(20100..20420)
                     /locus_tag="VXM85_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1345313.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20582..20662)
                     /locus_tag="VXM85_000690"
                     /pseudo
     CDS             complement(20582..20662)
                     /locus_tag="VXM85_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20706..22703)
                     /gene="metG"
                     /locus_tag="VXM85_000691"
     CDS             complement(20706..22703)
                     /gene="metG"
                     /locus_tag="VXM85_000691"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1345314.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22827..24086)
                     /locus_tag="VXM85_000692"
     CDS             complement(22827..24086)
                     /locus_tag="VXM85_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345315.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24088..24789)
                     /locus_tag="VXM85_000693"
     CDS             complement(24088..24789)
                     /locus_tag="VXM85_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345316.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24773..25972)
                     /locus_tag="VXM85_000694"
     CDS             complement(24773..25972)
                     /locus_tag="VXM85_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1345317.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26258..27604
                     /gene="gor"
                     /locus_tag="VXM85_000695"
     CDS             26258..27604
                     /gene="gor"
                     /locus_tag="VXM85_000695"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1345318.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            27801..28337
                     /locus_tag="VXM85_000696"
     CDS             27801..28337
                     /locus_tag="VXM85_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1345319.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(28538..28753)
                     /locus_tag="VXM85_000697"
     CDS             complement(28538..28753)
                     /locus_tag="VXM85_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1345320.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28851..29171
                     /locus_tag="VXM85_000698"
     CDS             28851..29171
                     /locus_tag="VXM85_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345321.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29202..29540)
                     /locus_tag="VXM85_000699"
     CDS             complement(29202..29540)
                     /locus_tag="VXM85_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345322.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29552..30271)
                     /gene="trmD"
                     /locus_tag="VXM85_000700"
     CDS             complement(29552..30271)
                     /gene="trmD"
                     /locus_tag="VXM85_000700"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1345323.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30261..30779)
                     /gene="rimM"
                     /locus_tag="VXM85_000701"
     CDS             complement(30261..30779)
                     /gene="rimM"
                     /locus_tag="VXM85_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1345324.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELTLFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30809..31597)
                     /locus_tag="VXM85_000702"
     CDS             complement(30809..31597)
                     /locus_tag="VXM85_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1345325.1"
                     /translation="MKRFEVSTEIGSLSVTYKKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGRNHHYLQWSESEKIAAIIRELSE"
     gene            complement(32174..32413)
                     /locus_tag="VXM85_000703"
     CDS             complement(32174..32413)
                     /locus_tag="VXM85_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1345326.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32433..32705)
                     /gene="rpsP"
                     /locus_tag="VXM85_000704"
     CDS             complement(32433..32705)
                     /gene="rpsP"
                     /locus_tag="VXM85_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1345327.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            32875..33084
                     /locus_tag="VXM85_000705"
     CDS             32875..33084
                     /locus_tag="VXM85_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345328.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDL
                     LADDSLAGDIILLWRIQFGTFTTET"
BASE COUNT         9801 a         7246 c         5945 g        10154 t
ORIGIN      
        1 gaggcctatt taccgtggac taaagttgta caagaaaagt gcaaataaga aatctccaga
       61 ttaggaacta tccgtgagtt ctctagtctg gagatttttc aatagacttc gttattggac
      121 ggttaccaag ttactcaaag tacagatttg aagctgcaga taagactgac gaaatcactt
      181 taatactaaa tgaaaatcaa aaagcaaact aagaagctag cctcaggctg tacttgagta
      241 cggtaaggcg acgctgacgt gatttgaaga gattttcgaa gagtataaaa cactgttttg
      301 aggtggtgga taaatcggct gggtaaaaac tcaatctcag gagaaaatga agtaaatctc
      361 cccgcaacta tagaaaaaga tttgattctg ctcctcacac aataaaatct ttttctttta
      421 ttaaaaacga atcgtttcac gtgttttctc atatgaggta acaaagcgat tagttacacc
      481 aggctctgcc acttccaatg cttgagaaat cttgtcaaat gaagcgtgat aatcaaattc
      541 tttttcaaaa atatctaaag cttcgttaaa tgcttcttga atgcgttcat caaatgagcg
      601 atagcggtta gaatattgca agagttgctc tgtcaaagtt gcatattgta caatattata
      661 agtttccgtt tctaaagctt ccatatcatt cgttgcaatt tcaagaactc gggtaacaga
      721 ttcaatgtta atcatttttt gttctaactc aaccattaaa tcctcggtat tattgcttgc
      781 cgtaaagaat aacttcaaga aagtttgtgg aatacctggc agattgcgtt tttccatgta
      841 tcgcttgata gtatggagac gattgacata aacattggcc ttttgacgtg cattaatatc
      901 atctttctca atttgtgtca ggcgctcact aactgaaatt tgctcatctt caatatcttt
      961 tagttgagtt tgtaaatcct caagattttc ttcaagaact gaataagctt gggttggttc
     1021 ttcttgattc gaagttacct caacaatagc tgcctcaaaa ctctctaatt ctgtctgaat
     1081 acgacgaaca tggcttgcag ctgtctcagg aagtaaatag gtcttgttca aacgtgcaat
     1141 atcttctccc aataaagtat tattctcttt catatgttga aggtaagttg gaagagttgc
     1201 aagtagattt tccactactt tctgagcagc aatttctcga gtaaaaatat catacaaggc
     1261 attgatttcc tcttgtgcct gtccattctc atattcggca ttatccaatt ccaactgacg
     1321 aatattctct tggtttttct tgaatgcttc ataaagcaag tggaaacgcg cttcaatatc
     1381 cgtttcaaca aaatgataat tagcatcaat tagtttacga taaccggctt ccaaatcctg
     1441 taattgatct ggcaattctg tagaaagcgt cgtaaccaag gctggaacac gatccacaat
     1501 atgacttaag gccaaaatgt gattttctgt attatccaaa atcactgcgg cttccacagg
     1561 gtcacccgat gaattcaagg ttacaaattg tgaaaattca gattggatat tttctaattg
     1621 tttttcaatt tcatccaagg cttgaccata ctgttctgaa ttttcagcaa ctctatgctg
     1681 aagttcctca aataaatcca aagcatgaag aacacgacca ctatttttag attcttgctt
     1741 ctctaagtct gccaaagcat tgcgaattgc cgcaatatct tcttcaatca aagtaatttg
     1801 actctcaatt tggtcaattt gatgactggc cttgagaaaa cgaaatgaat ggttatagct
     1861 ctctgcttca aagagattat tttcaatatc ggcaaaagag ttgagagata aatcgaccca
     1921 tttttgattc cattcacgga aagccacttg actttgtcca atcaagtgca tattttttac
     1981 agcttctact tcatcattta ctggaagatt gtatagttct tcttttcttt cttctagtgc
     2041 ctctaatctc ccctcgtttc gcttacgtag aaagattgcc actacataag ccagaactaa
     2101 aatgactgca attgcaacca ttaaataaat tagttgtcca ttagacatat caaactcctt
     2161 ttttacttga aacaatcgta acgattatat catatttttt agaccaaggg aactacttct
     2221 ccctattttt tagacatcaa gtgtactata gacagcattt tcttcgataa actcacgacg
     2281 aggctctact cgatccccca tcaacatatc aaagatttta tctgcttctg cagcatcatc
     2341 tacagaaact ctagccatca agcgatgttc gggatccatg gttgtttccc acagctgatg
     2401 atcgtccatt tcacctagcc ccttataacg ctgaatagtc ggtttggtac gaccttcact
     2461 ataacgggct aaagcttctt ggagtttgat ttcttgatct gcacccggct ggatatattc
     2521 tttaatctcg cttccaacct tgacaccata gattggtggt tgggcaatat aaacataacc
     2581 agcttctagg attggtttca tataacgata aatcaaggtt aaaagaaggg tacgaatgtg
     2641 ggctccatcg acatcggcat cggtcatcaa aacgagtttt tggtaacggg ctttcgaaac
     2701 atcaaattct gcgccaaatc ctgttcccat ggctgtgaaa agactacgaa tttcttcgtt
     2761 agctagaatc ttatccatac ttgctttttc aacgttcaaa atcttaccgc gaattggaag
     2821 gatagcctga aactcacggt tacgaccaga tttggctgat ccaccagctg agtctccttc
     2881 gacgatgaag agttctgttt cagcagggtt attagaagaa cagtctgcta gtttccctgg
     2941 aaggttggaa atttccaaac cagatttttt acgtgtgact tcacgcgcac gcttggcagc
     3001 cacacgagcc ttggcagcca aaattccttt ttctacgata cgtttggcaa tctgtggatt
     3061 ttccatgagg aaatcggaga aggcttcact gaagaggcga ttggtaatct tgaccacttc
     3121 gctatttccc aatttggtct tggtttgtcc ttcaaactgt ggatttgggt gtttaactga
     3181 gataactgca gttaagcctt cgcgaacatc ttcccctgtt aggttgtctt cattgtcttt
     3241 cagtaactta tttttacgag cataatcatt gataacacgt gtcaaggctg tacggaaacc
     3301 ttgttcatgc gttccacctt catgtgtatg aatattattg gcgaaactca tgacattttc
     3361 atggtaacca gttgtgtact gcatggctac ctcaactgtg atatcatcca tctcaccgtc
     3421 tgtatagatt ggtgtatcaa agattacatc cttgttctcg ttgatatatt caacgtaact
     3481 agcaatccca ccttcataat gataatgctt ggtttgttcc aaaccttggc gcttatcagt
     3541 gatagaaatt tgaagaccgc gatttagaaa ggccaactct tgaatccgtt tatttaattt
     3601 atcaaaatca aagattgttg tttcagtgaa aatttctggg tccggtgtga agtgaaccgt
     3661 tgttccagtt ctatctgtat ccccaaccac ctcaaggtct gcaacaacat gaccacgacg
     3721 gtattcttgg taatgaatct taccgttttt atggacatga acgtctaatt gagtggaaag
     3781 ggcattaact actgacgacc ccaccccgtg aagaccacct gaaaccttgt atccaccacc
     3841 gccgaacttt cctccagcat gaaggactgt aaagacggtc tcaacagcag gacggcctgt
     3901 tttttcctga atatcgactg ggataccacg cccatcatcc acaacagtaa tcgaatcatc
     3961 tggctcaata aaaacttgaa tatggctggc aaatcctgcc aaggcctcgt caattgagtt
     4021 atcaacaatt tcccagacta gatggtgaag accttctttt gaggttgatc caatgtacat
     4081 ccctggacgc atacgaacag cctctaagcc ctctaaaact tgaatttgac tggcatcata
     4141 atcctgtgcc tgcagatttt tgatttcttc tgtcatctta ttcctttttc ttatatgtct
     4201 aatacttgtc ttaaattcac gatactggta aacaagtggg tatcaagtcc tgcagcttga
     4261 cctgcttcga tatcaatcgg ccgatcacca atgacaagac cagagctaat ctgatacttt
     4321 tctcttaaat aaagcatgga ttcgggattt ggctttctct taaagcctga gctagaagtc
     4381 accacttctg taaaataagc tgctatagag gttttttcta aaatttccaa aacctgatca
     4441 tttcgatgag agaccaaaaa atgacggcca ccttgatttg aaatgacttc caataggtca
     4501 gaaactcctt caaataaaat cgggtgttca agctctctgg cttcattttc cttgtacttt
     4561 tctaaaaaat tctctaaatt gggagcgaat gtctcaatcg caaaagaagt agaaaccttt
     4621 aaagcttgat agacactgtc atggtcttgt gtgataccat acagtgccaa tgtttcaaca
     4681 aatgcagctg ttgaagtttc ataattatcc agtaaagttc cacctaaatc ccagatgtaa
     4741 tcgtgatatt tcataccctt cattatagca tttttttatg aaaaaagcga tgatttccct
     4801 gagttttcca cataaaaaac gagagaataa ctcccgtttt actgattttt accaaagaca
     4861 tctcgataga gcattcctgt tcgtaaactc tctgcgtccc ctcctagttg ctccaccaac
     4921 tcgtaggcaa aggcaagggc tgttgaagga ccccgactgg ttgtcaactg accatctacc
     4981 actactgttt ccttgacgta gtgaccatca aggatttgct cttgaacgcc gtcataacaa
     5041 gtgtatcgct tatttttcaa tatctctgct tgattgaggg caattggtgc cgcacaaatg
     5101 gctgctagtt tcttcccttc ttgctcgaag ctttgcaatt cttgaatcaa ggtctgatta
     5161 tcacgtaaat gtgcagaacc aggcatacct ccaggaagaa caatcatatc atagtctgat
     5221 aaatttccat caaagacatg atctgctctt acttggattg catgcgaacc cgttacttgc
     5281 tcttcaaaac caaccatatc acatgtgata ttggctcgac gcaagacatc tacaactgtc
     5341 aaggcttcaa tttcttcaaa gccctgagct aacataactg ctacttttac catgattttc
     5401 tccttattta atttgtttta atacaacctt tttccgtttg aatgggactt ttccgctctt
     5461 ttcttcagct agattagtct ggtaactcat cgataaaagc aaaccaacac caatcagatt
     5521 actgataata gctgatcccc cttgcgaaat gaaaggcaag ggaatccccg tcaaaggaag
     5581 tagtccagtc acagcaccga tattctcaaa gatgtggaag agcaacatca taatcaaacc
     5641 tgtggaaata taagtgtaga actggttatt tgatttaaga gtaatcttca acatacggta
     5701 aatcaacatg agatagaggg caataaccag gacagagcca ataaagccaa aatcttctgc
     5761 aataaccgta aaaatcatat ctgactctcg aactgggata agcagattcg aagcattaaa
     5821 tccctgacca aataagccac cactcccaat ggcaatctgc ccttgagcct gctggtaagt
     5881 cgttgtttgg gcaaactcaa agggattgag ccaagccaaa atccgattga tttggtaggt
     5941 cggcattcca atctggtgaa gaaaagctcg tccgtccttg ctaataaaga tagctaagaa
     6001 accagcaact cctgttacag cagtcacaaa tactgggata ataattttcc aagaaacccc
     6061 tgataataaa acgattcctg agaaaatggc tacaaaaacc aaagccgtcc ccaagtcact
     6121 ttgaagtgct aaaagaacta ggactggaat ggtaaagaga atcatccaga aaattaacaa
     6181 aaagtccagc ggaaccgtgc gtctccattc cttatgtttc tttgtaaatt ggacaatgac
     6241 acgagccaac atgaggatat aagatatctt cataaattct gacggttgga atagggtaat
     6301 tccatttatt gatacccagt ttttggcacc cgttgatgca actaatcttg gattataaaa
     6361 tacaatcggc aagatcataa gtcccaagcc taaaatatat agaaaggggg tcaccttcca
     6421 aagaaattct gtattaaaga gcatgaccac aaaaccaatc acaagcccca aggcaatcca
     6481 ggcgacctgc tgccctaaaa tgggcaaaat attattggga taatcatgac taacggctat
     6541 atagatagcc accacaccga tgaccagtag aaaaaatact ggcaagagca aactgtaatc
     6601 gactcttgag tcgagagaac gtttcatata tactaacctt atactttcat acaatactat
     6661 ttatcaaagt tcattaaaaa atctatcaat agcctcgtca acttcggatc gagagatggt
     6721 tttaacagtc gcttcttctg ctagagatac tactatttgt ttgccgtatc gttttccgac
     6781 gattctccta tccaaaataa gagttaagga acgttggtat tcacgtctca tacttcttcc
     6841 caaagcctgt tttaaacgaa taatggccat tggcaattga taatcataaa aggcattttt
     6901 cccttcttga ttcagttctt gattaatctt tttcgtcaag ggttcttgag gattttggaa
     6961 aggaagcctc ggtacaactt gaatcacaaa aggatggctt gaaaaatcaa ctccctccca
     7021 gaaacttgct gcaccaagca agatttgttg ttcacctttt tcaaagcgtt tctttagctg
     7081 atgaacatcc ccatttttat actgggccaa gtggctaact gtaagtaaat ccgatactgc
     7141 tagaagcatg tctttagcgg taaagagaac caaaatcggt tgctggaaag cttgaatttc
     7201 cactagtaaa gcagctacct ctctggcata gacttctaag gaggtttctg ttaccagagg
     7261 gaaatctttg accatgacca cttcttgttc ctgttttccc cgagattcaa tcttgacaaa
     7321 tttggcttca ggatagccta aaaggtctgc caaagaaacc ctctgactaa tctcaagagt
     7381 agccgatact cccaagactt ggcaactctc aggaagtaaa gaggacagaa gagtacggca
     7441 tgattttgta gaagaaatct gaattttctt ttgactcgtt tcagttactt caagccagta
     7501 atcaccttca gctgtaaaat agcgaaccag ctcatcaaag ccttctactt ctaattctga
     7561 aaaatactga tggagattgt ccagagaatc taagatattt ttcctagatt taccagactg
     7621 aaattgttct atcaagtaga gacactcaaa gcgaatactt tctagtatcc gttgttgaac
     7681 cctgttttct tctcctacta aagccttatc aattaaatcg ataatagatt gtatatcgta
     7741 ggtctcttga agcagatttt ctagagctaa caaaatcttt tggacttcat caataatcag
     7801 taaacggtca ctgacaaatt caggattatc ttcaagtctg gtcacgagat aggcatgatt
     7861 agtcactaaa agcttgcaag tctctgccct ttcttgacta cgtttccaaa aatcttccgt
     7921 cacaaataag ctctgggatg ataaattccc atcatgacga aggtctgcta gaaaatgttg
     7981 gtaacggtag agttgcccga tttcatccaa atctcctgtc tctgtctcag taagccagac
     8041 caagacttgc attttaaagc gtctaaataa gcgattttca tcattttcct gcaaggaatg
     8101 ataaaaggca tccaacttca gataattttg tggtcccttt aagctatgaa tatctgtatg
     8161 gaacacttcc ttgaggcgtt taccttcttc ttccatgatt tgattttgaa gaatctttgt
     8221 cggaacacta agaacaattt gtcgctcttt ggattgagat aaagcgggta agagatagcc
     8281 ataggttttc ccaatccctg tcggcgcttg aatcagagag acaggttcat ctttcaatag
     8341 caagccaacc tctttagcaa aactttcttg ttcctccctc acttcaaggt tcaacagaga
     8401 aatattttta gaaaagtctt gagatagttt tcgtggctcc agagaagctg ccgttttctt
     8461 aaaatacaga ccttgaactt ggaccaagtc tggagaactc aggatagatt ggttgcgata
     8521 agtttcctca ataaccaggt aggactcata taggagagcg tcagccattt ccagcaagcg
     8581 ttccaagaga cctttaggaa gctgggtcat cttttcccgt aaaaaaagaa gtaattctgc
     8641 tgtagcttgg gcatctgaaa gggctgtgtg tgcgtgttta agaggaattc ctaattctcg
     8701 acacaaaatc ggcaagctat atttttccag ttcagggaaa aagacctggg ccaattcgac
     8761 cgtatcaaca cgagggtttc ttagctcata gccttcaaaa aataaatttt ccgccaagag
     8821 attagcatca aactgaacat tatgggctac aaaaatccca tcctccacca agtcaaatat
     8881 ttttctggca acttgcgaaa aatcaggtgc ttgcgccaga cgttggtctg tcaatcctgt
     8941 cagttctttg atatgagcat ccaagggttc atgtggattg acatccgtcg tatagtgatc
     9001 gacgatttct ccgtcctcaa tcacgacaat tcccacttgg ataattttag ccttactacc
     9061 tgtgctagtt gcctctaaat ccaccacaac atagcgatta ccaatcgttt tcattataaa
     9121 aaattatacc aaaaaaaggg ccatttggca cctgcaaaca taggataatt ttcaaactca
     9181 agaacactac ttttcttaaa aatcccatcg aatagagcat tctagcctgc tgatttttgt
     9241 aaaatagtga agaaatacga aaaattgaga gaagaaatgt ttgcatcttc cttctctcaa
     9301 cgaatcatgt attttatcta accatgtcag gatcgtagtc ataaaatcct gtatcaagga
     9361 aacggttttt agggtgaagt ttacgaactt cttcatccag caagaaggct tgatcatggc
     9421 taaagttgcc ctcttgaatc aagttacgcg cttggataaa gagttttgca atctcaacaa
     9481 agttctgacc aggagtgtag agcccttcta gtttccagcg agtaaagcca tgctccacca
     9541 attctgttaa tttgatcatc aaatcaaggt cattgttggc aaagatatgg gtcccatgat
     9601 tatcttcaaa aatggaatag tggctctctg gatcacttgg ctcagccaag aagaggtcac
     9661 gtttatgcgt cttttcatca tcgatatgtg taaagttata gtagttttgc aagagtggac
     9721 gtttagaatg atggatgacg ctagcaccgt aaaccaaaac ttcagcagga atttccaaaa
     9781 tctctggcat tttgaaaagt tcagctgatg gaatttcacg cgccaaaaca gcctcagatg
     9841 cgccagcctt ttgtccccag aagttaatct gacgactgct agttaccatg gttgaagcat
     9901 cgtagatggt cttaaatgaa taaccatcgc ggttaactac gtaaaagacg cctgcatccc
     9961 caatcgtaat atagtctgtc ttgatttctt ccaagaagtc taagaaaggc ttgatacggt
    10021 ccatcatatc ttggtgcatg agagcattga ccgcaacgat caattcctta ccagcatcat
    10081 gaaccaactt agcgatttca cgtaattggt cataactaaa ggtcgttggc agacgaaggc
    10141 caaaatcttt ctcaccgaca tagatacggt ctacgccagc ttcgagtagt tgttcaactt
    10201 gttcaatact ttcagcagtt gctgtaatga caatcttttc cataagaaaa attataccac
    10261 atttctccaa aatcatccat gctttgaaaa tgaaaaggga ctttattctt tttgtaacct
    10321 ttctgtaata attctctgcc gaaaaaatga taaaataggt atgtactgtt aaggagagaa
    10381 ttatgcccgt aagaaaatta caatcctatg aggtagacta tcaagaagaa ttaaaccagc
    10441 agcttcctca ttaccaagct tatacacctg aagcgcaatc tgatgccaat ctcaaggaaa
    10501 tcctattttt tattaatatc gctgtttttt gtatctgtat tgctatcttt agttttatct
    10561 ttttagcatt aaaattatca actgctcttg cctttgccgc agcaatcgga ttcagcttac
    10621 ttgttttaaa agttcaacgg tctattatca aacgaaaacg tagaagataa atccaaaata
    10681 tcgccccagt tggagcgata tttttatttt ttctttttag atggtgtctg aatggcaatc
    10741 ttcacttcaa agattgttcc cttgggttta ttatctttga cagtaacagt tccttttaga
    10801 gcatctacaa tttgcttggc tagggataat cctaaaccaa aaccaccttt ttgccgggtt
    10861 ctagccttgt ctactcgata aaaacggtca aaaattttct ttttatcttc tgtcgaaata
    10921 ccgattccat tatcagaaac aagtaaataa agattgcgat cggtcgccga gataagaaaa
    10981 tcaatttcac catcctcctc agtatacttg acggcattat cgaaaagaat ggtcatcagt
    11041 tgtttcagaa gaagctgatc tgtgacaatt gttcgatgga tacgattttc aaaacggaag
    11101 acacgattat tttccgaagc aatcatctcg tagtttgtga aagttgtatt aaaaaagcta
    11161 gttggaactt ctgcaagctc cggcttaatc ccatcatctc tccgagctaa gttcagcaag
    11221 cttgtcgtta aaaaacgcat atttcggact tcttccaaac tcgatgcaat gctttcgctc
    11281 acatccataa tggtagcttc tggcttacga aaaagggtct ctaagcgatt ttgcaaaact
    11341 gcgagtggag ttcgtaactc atgactggca ttttccacaa aagactgttg cttctgcata
    11401 ctctcaagca ggggcctaac actgacccta gctagataga gactggcaag taaagacaaa
    11461 atccagaaac tagccatcac gaccacaatc aattgctcat gcttttgact agcctgttcc
    11521 aactgactgg tattaatcaa gacagcagca tacttgatat tggttgaaac cgaactaata
    11581 ttggtctcca tcaaaatcac acgatagatt tcttcctgcc catagctatt aaaaacctga
    11641 atctggtaga tatgtcctag ttctttcttt tctaacttaa tcttatccaa gcccaaaaat
    11701 cgatttccag aaagaagttg agtaaagtct ttatcaaaga gaatgacttc cgtattggaa
    11761 ctgacattag gttttatttc tactttacta gcgtccgccc tagcattttc taaatcttta
    11821 atctcttctg ttgccctatt tatagccagc tgaataactg cttgaggatt ttcactcagt
    11881 ccatgaagct tatcgtccac cgaagtataa agactcgaat gcatgacttg taaaataatc
    11941 agagtcattg tagaaaaaat cagggtgaag acaccgaagt tgcggataaa ataactaaag
    12001 tcatccgcat accatgtttt tttaagttta ctgaacatct tttaaaagat acccaacact
    12061 acgcaaagtt tgcaaattct ctgcaaaagt ggttcccttt aatttcttac ggacttttga
    12121 aacatagact tcgacaaccg aaatcgttgt atcactatca aatccccata gacggtcaaa
    12181 aatctgcgtc ttaggtaaaa tcacattttg attttgaagg aaataaacta gtaaatcgaa
    12241 ctctttcccc agcaattcga caggagtatc ttcaacttta acggtattgg ttgataaatt
    12301 aaccacgata ttcccataag tcaaggtgtt ttcattaaac ttccctgaac gtttgagaag
    12361 agcctgaatc cgcattttaa gttcttctag gtagaaaggt ttggtcagat aatcatccgc
    12421 tcccagttca aatccatgtc ccttgtcatc caaactttcc ttggcagtca taatcagaac
    12481 tggtgtcgta attccctttt cacgcaattc ttttaagact tggaaaccat ttttttctgg
    12541 caacatcaaa tccagcaaaa tcaagtcata gacaccactc tcagcttcgt agagaccttc
    12601 ttctccatca aatacctgca taacatccgc aaaatcgtct aaaaagtcaa atactgaatt
    12661 tgacagacct aggtcatcct caaccaataa gatttttatc atgagaaact cctccttatt
    12721 aaaactatta taccaaattt gccttaaaaa aaactcaact ctctgcattt tacatgagat
    12781 agctgagttt tctttttatt ttaggcttat ttatgcattt ccgtattgaa gaacaactgc
    12841 ttcgactgca gctttttcac ggctaatcaa gtcaacacgc gctgcaattt ccttgattcc
    12901 cataccgatg ttacggctaa gagcaaggtc agaaagttgc ggctcaaaga actccttgta
    12961 ttccgccaag cgttgctgag tcttaaatac atgagcagga aggataacaa agctatcaaa
    13021 gctcatatct cctccaaggg ctgccttaat ccaagcccag ttttcacgcg cccaagacca
    13081 agctgttttc tgagttgctt gatgagctag gaattggtaa taccaagcag acaagtcctg
    13141 tggtttgacc acaaatttgt ccttccaaga agtaatcagg ttttggatat tatccgcatc
    13201 tgtactgtat gcaagagctg ctgccaactg gcgtttaaag acagcatctg ttgcgtgagt
    13261 ataagtatca agataaagtg ctaacaagtc tttagtctca tgatgtttca tctcattaat
    13321 cagaacttgt gagcgaatag ctgctgggag tcctgcaaga ttctccttgt gtgttgcgaa
    13381 gatttggcta gcgacttgac tagcttctgc atcatttgag cgaatcatca tcgaaacagc
    13441 cagctgacga accaattcat cctcatctga ttctccgtct ttagcttcaa aaccaagacg
    13501 gtcatagtta tgacgagcca atttagcaac cagtcctttg aaggctgttt cagcatccgt
    13561 tccttcatca ataaagcgct caagggctga aatcacttga gaaacagctg aaaccaccag
    13621 ataagactct tccttagcaa gtttatcaag aactggaagc aagtctgcat aagaaatgtg
    13681 ccctgcctca gccaacaaac gacgttcttg aacaatttgc agtttgcttg tgttatcaag
    13741 tgtctctagc tcagcaagaa cagcttctaa caagtctcct tgatagtcgg taatatagtg
    13801 ggcagtattt tcagtgttga gacgaagagc tccttcattt tcagcaagaa gagctgcgta
    13861 gccagggatt tcgatacttt cagtttcgag tgtatcaggc aagcctttcc agttgctatt
    13921 gaggggcacc acccagagac ggttcttgtc ttcgttctca ccgatgaaga attgtttttg
    13981 tgaaatcttc aagacatcat tttcaacttt aacagtaaga actgggtaac caggctgttc
    14041 caaccaagaa tccatgaagg ctgcgacatc acgtcctgac gcttgaccaa gggcatccca
    14101 aaggtcacta ccaatggtgt tgctgtattg gtgtttttca aagtaggcgt gcaaaccttt
    14161 agcaaaatca gcatctccta gccaacggcg aagcatgtgc atgagacggc ttcctttggc
    14221 atagacgata gcgccgtcaa agagtgtatt gatttcatct ggatgtttaa cttcgacgtg
    14281 gacagactga acgccatcag tagcgtcacg ttcaagagca agaggtactc cacctgtttg
    14341 gaaatcttca aagatattcc agcttggttc gatggtatcc acacagacgt attccatcat
    14401 attagcgaaa ctttcattga gccaaaggtc atcccaccat ttcatagtca cgaggttccc
    14461 aaaccattgg tgagccaatt catgggccac aacaagggca acttgttgac ggctagcaaa
    14521 tgtagagttc tcatcgacaa ccaagtaaac ttcacggtag gtcacaagac cccagttttc
    14581 catagcacca gctgagaagt caggaagggc gatgtggaga gattgaggaa ttgggtactt
    14641 aactccatag taatcttcgt aaaactcgat agagcgaaca gcgatatcca gtgagaaatc
    14701 aagatttgaa agtggatgtg ctttggttga gtagacacct accagggtac catttttagt
    14761 tttagcggtc accccttgca aatcaccagc aacaaaggcc aacaagtaag aagacatgcg
    14821 aggtgttgtc tcaaacttcc agatacctgt ttccttacgg ttttcaacat cgatttctgg
    14881 catgtttgac aaggccaatt caccttctgc ttggtcaaag cgaagagaga ggtcaaaagt
    14941 tgctttggct tcaggctcat ccacacatgg gaaagcttcg cgcgcaaaat ggctctcgaa
    15001 ctgagtagac aagacctcct tcttgactcc atcaactgta taataagaag ggtaaatccc
    15061 tgtcatgttg tctgtaattt taccagaaaa ggcaagaacc aattcaactt gaccagcctc
    15121 agccaattcg atatgaaggg cttcattgtc atggtcaact gtaaatggac gagcttgacc
    15181 tgcaacttct acagaggtga tttccaagtc tttttggtgg agggagatgc ggtcactctg
    15241 tgcttgacca gtgatggtca ctttcccaga aaaagtcttg gtctcacgac tcaaatctaa
    15301 aaataaatca taatgttcag gaacaaattg cttaataaaa tgttcaactg cttgcatagt
    15361 tttctcctat tctaagttta agagctgctg ctcttcttca aacaaactta ttatatcata
    15421 ttttgcttga gaaaaaagga ttggacagcg atttccaaaa ctttcttcct tctaatagtc
    15481 tacagtgggt agaccttgcg aaattcttct aaaacaacct tgctttcagg tgtaaaagtc
    15541 agccctactt cacccaaaca gtctgtgaaa aagtcctcat gaacctggcg ccaataggca
    15601 agtgatttgt ctccctcacc ttccttaaag gcatgttgcg cagaaacttg attgaagagt
    15661 tcaacagaaa cctttgtaat ttcgacaatg cagacagact gattttgact atctaaaatg
    15721 acatcgaagg tccctacttg gggaagtggt tcgtcttcta gtacatagag gtcataggct
    15781 gatgctgttg ctgtcttttc tcctttaaac accaaatccg ctaaaaggtc tggttcaact
    15841 ccaaaagccc aagcatcgat ttcatctccg atcaaaggat tgatttgctt gtatttattc
    15901 cacatttctt gcggtatcat gggtactcct ttgtaatttt ttactttctt cttttatgtg
    15961 tttaagatga tctggatggt caatctctaa atcaaaaatc tctggaatag aactgtagtg
    16021 gataatgcac ttgataccca actgattcat tttttgtatg aaagaagtat tcagatagcc
    16081 tgctacagca aaatcaatct tgttctttct tgctttatcc tgcatatctc ttagcatatc
    16141 taacattatt ggactttcca tatcatgcca ttgactgttt ctcatagtcg caaaaacaaa
    16201 ggaagtcaaa tcattcattc caactacaat ctttgaaatg cccgtttcca gtatcctatc
    16261 caagtcaaaa tacgctgacg gtaattcaat catcgttccg actttcccag taaagccata
    16321 ctgacgcaat actgtaatag cttgttttaa ttggtcggca tcattgacaa aaggaaagat
    16381 aagagataga ttgggattgg tttgataaac ttctgtaacg acatgtgctt cagcctgaaa
    16441 ttcatccaaa cacgccagta aacgcctggt tcctctatag ccaaacaagg gatgcccttc
    16501 gtcaaaaaac tctctagtcc ccactaaaca attggcttct gtattcgtta attcagaaaa
    16561 acgataccaa acttcttcat ctgagtacag ggagcagata gtctctagat aatcttttac
    16621 aaattgctga caacttgcta atatgatgtt ttgattaagc tctctcaaca aatattcccc
    16681 acgaatcatg ccgacgtggt gaaatagttg aggataaatt ttttcaagaa ttttttcgcc
    16741 actaagggca agttgatttc tcatcattca ccttccaatt catgtaagaa gtcttgtcca
    16801 gttctggaaa tcctaataat tcagacttaa ccttcaagac taatggcgat gcattttctt
    16861 ctgtaatctc ttgaatatcc atccaaatat atccaagtga atcattcgca ccatcagaca
    16921 cagcttccga agtcgtaact tgaggtgcac tctcattcat ttcaacatca tacaaggcca
    16981 tgacatggtg aaccataaaa tttgttaact cttctctgac gaaaacatcg tagattcgag
    17041 gattagagta gcttctaaca gtaaatcccg tctcttccat aacttctcta gtcagcgttt
    17101 ccgtcagtcc ttcaccaagt tgctgactgc ctccaggtag atcataccga tgttgataag
    17161 ggcctctcgt tttttcaatg cagagtaact tcccattttc taagcaaaca gcgtagactc
    17221 caaagtgttt tttgatttcc atccaactcc tcctacttca aagaccagcc accatctatt
    17281 gtcaagattt gtccttgcat ggcgctcgct tttccacttg ctaaaaaaag actaagctct
    17341 gctatttcct ctggctcaat ccagcgcttg attggggttt cactagccac ccagtcagcc
    17401 aaaccacctg gttcaaaatc cgcagcggtc atagctgtct tgactgctcc tggagcgata
    17461 ccaaagacct gaatcccagc ttcagcatag tctagagcca actgcttggt gaagccagcc
    17521 aaggcatgct tggatgaagt ataggcgtga ccacctccac ctgctaggct agaagcaatg
    17581 gaacacatat tgatgatgat tcccttttta ttttccagca tttgtgtcaa ataataccga
    17641 gtcaactcaa caggagtcat gtagttaatt tcaaaaatct cttgaatttc ctgcgcagtc
    17701 tgttccaaca gtggtttgta agcatccaaa actccggcag tattacacaa aacatccacc
    17761 tgagggcacc agtcaaaaat aggttccaag tccaaggtca aatctctctg taaaaagtga
    17821 aaatcaccct ctaagagtgg cttttcacct tggtcaactc cataaacttg atagcccttc
    17881 tctaaaaaga ggcgagcttg agccaatccg atccctgaac tcactcctgt aatgagtaca
    17941 cgtttagtca tgcacttcta cccaatccgt tgccaaaaca tcacaagctg tcgggctcca
    18001 catggaaaaa ccttctcctt ctccagacac gttgattaag aaataaggtg tcacttcaag
    18061 tgcaagccca ttttgctcga tggtatcaaa gagttggaca tagttttcag cacctcccca
    18121 accagttcgt acatattttt tcttagcctt taagccaggt agaatttctt caaatgtcat
    18181 gtttttctcc tttaatgtca ataattctcc ccttcattat aacaaaaaaa ccgctttaaa
    18241 acggcttttt gactgtgatt tatttacatc ttcttctact tacggcaaat tattccctgc
    18301 agcaagataa atttcatacc attcttttct tgttaagcta aagtttgccg cttggctaac
    18361 ttctctcaag tgcttaggat ttgtggtacc tacaactgcc tgcatttttg ctggataacg
    18421 caatatccaa gaaatggcaa tagttgaaga ggttactcca tatttaatag ctaaacgatc
    18481 aagtacttga tttaaagctt gaaatttctc atttccaaca aagttccctt taaaataccc
    18541 gaattgcaag acagaccatg cttgaatgac cacatcgtgt aatttgcaat attcaaaaat
    18601 gctgccatct cgcatagctg cttgactatc ttccatatta acatgaaaag ctgattcaaa
    18661 tcctggagta aaagccgcac tcaattgtag ctgattaaca gctaacggct gcttgacatc
    18721 ttttttaagc aactccatca tcataggatt ttgattagaa actccaaaat ctcgaacttt
    18781 accttgttta taaaggagat caaaggcttc tgctacttgg tcagattcca tcaaagcatc
    18841 tggtcgatga aggagcaagc tatctagatg atcaatcttc aatctttgca aaataccgtc
    18901 tactgatttt ataatatagt ccttagaaaa atcaaaatag gtaaattctt caatgcgaat
    18961 gccacatttg gactgaatcc acatcttttc tcttaaatct ggacgatttt ttaggacaag
    19021 acctaacagt tcttcacaac gaccacgacc ataaatatca gccaagtcga aggcattgat
    19081 tccaacagaa agtgctgttt ctacaagctc ttcaacttct tttacagact tatcttttat
    19141 tctcatcatt ccgagaacaa tttctgataa ttctttgtca tcttgaccaa gagttatgta
    19201 tctcatcaaa tttttctcct ttaatttcta acattcttcc cttcattata acaaaaaacc
    19261 gctttgcaac ggctttttga ctatacttca ctccatttta tcttcttaaa cccacggaac
    19321 aagacaaaga ttccaataaa gaggacagct aaaggaataa cttttgtaag gaaaacattt
    19381 gaaattccca tccactcata ataacggagc agagaaccca ccacaagatg ggcaataatc
    19441 atactgacaa atggacgaaa gaccgcttct ttccaattcc aaataccgat aactagcgaa
    19501 atcgtaaaga cagacaaact atcccaggga gccggaatat aaaaggctcc ttcttgtatg
    19561 aagcttgcca ttcctacata tcctaaaaca actagaagaa ctatagtccc aacaacaatg
    19621 taagtgccaa ttttcatttt aggagaatct tggactaaac ttcttcgtaa aattgtggcc
    19681 acaagtccaa atccaatcag aaaaataaga agttgcccta aaaatgtgag caaattgact
    19741 gttaagagag gacctttaga aaaatcactt agtagttgat aataacgtaa taccgccagg
    19801 acaagaattg gcgtcaaaag ggactctttg atagaactgc gaggtgctcc cttgagaatc
    19861 tctttcatta tttttttagg attcttacct agataatcct ctgcactcat gccatctcgt
    19921 tctgcttctg agaaatctag catcatcaaa tagatctgct ctctgagata gtcttcatca
    19981 tagagaaatc cagcaagatt aaaactttcc cacaactcct caaaatactt ttgattctcc
    20041 tcagaaaact catgtagcaa agcgcttgtt tcttcgtaat acttcatttt cttcatggtt
    20101 taacccccat tcttaatccc ttctactttt tgactcaaat cgtcccattg ttgccaaaag
    20161 actgagacac gctcttctcc ttctttcatt aatgaaaaat acttccgatc tggaccatct
    20221 ggcgacgggc gcatgtcgcc tcttatccat tgattttttt ctaacttttg caacaaagga
    20281 taaatagttc ctggaacgat agtatcaaat ccagcctctc gcaaagtctg aaccaactca
    20341 taaccatacc gctctttttg accaatcata tccaagacac aaccttcaag aacacctttt
    20401 aatagctgag tttctttcat cacttctccc ttctaatcta ttttgtaata cctactagta
    20461 acttcaccta tagtatatca cttctacact agtttgtaaa gcataatagt taatactctt
    20521 cgaaaatctc ttcaaaccac gtcagcgtcg ccctaccgta tgtatggtta ctgacttcgt
    20581 cagtttcatc tacaacctca aaacagtgtt ttgagcaacc tgcggctagc ttcctagttt
    20641 gctctttgat tttcattgag tataaataaa aaaacagaac tagtctgaac tagtcctgtc
    20701 tacttttacc caatcacact tccatttggt acagctggat caactgtgag aagggttaat
    20761 ttgccatcat gttcagctga tagaatcata ccctggctga catatttttt catcatttta
    20821 cgtggtttga ggttagcaac gatttgaact ttcttgccga ccaattcttg ttcatttgga
    20881 tagtattttg caattcctga aagaatctga cgatcttctc catcaccagc atccaagcgg
    20941 aattgaagca acttatctga accttctact ttagacactt ctttgacttc tgcgacacgg
    21001 atttcaacct tgtcaaagtc ttcaaacttg atttcatcct tgtttagttt gagctcaact
    21061 tcgtccggat tccattcttt ttcgactgct ggtttattgc cttccatttg ttccttgata
    21121 taggcgattt cttcttccat atttagacgt ggaaagatag gtgttccttt ggcaactaca
    21181 gtcacatctg ctgggaagtc agccaaactc aagttttcaa gactagaaac ttcttccaaa
    21241 ccaagttgag tcaaaactgc acgactagtt tccatcataa atggttcaat caagtgagca
    21301 actacacgaa tgctggctgc caagtggctc atgacacttg ccaattggtc acgaagagct
    21361 tcatccttgg ccaagaccca tggtgcggtc tcatcgatgt atttattggt acgagagatc
    21421 agagtccaga ctgcttcaag cgcacgtgga tagtcaactg cttccatgtg tgtatggaag
    21481 tctgcgattg attgttctgc aacctcagca agaacatgat caaattcagt cacaccttct
    21541 acataggcag ggatttgtcc atcaaagtac ttattaatca tggaaaccgt acggttaagg
    21601 aggttcccaa ggtcattagc caattcatag ttgatacggc cgacatagtc ttcaggagta
    21661 aaggttccgt ctgaaccaac tggaaggtta cgcatgaggt agtaacgaag tggatctagt
    21721 ccataacgct ctaccaacat ttcagggtaa acgacattcc cttttgactt agacattttt
    21781 ccgtctttca tgacaaacca accatgggca atcaaacgat caggtaattt aacatccaac
    21841 atcataagaa ggattggcca gtagatagag tggaagcgaa ggatatcttt tcctaccata
    21901 tggaagactg ttccattcca gaacttgtca aagttaccat gttcgtcttg agcgtagcca
    21961 agagctgtcg catagttaag aagggcatca atccaaacgt agacaacgtg ttttggattt
    22021 gatgggacag gcactcccca tgtaaaggtt gtacgagata ccgccaaatc ttccaagcct
    22081 ggctcgatga agttgcgtag catttcatta aggcgaccat ctggcgtgat aaattcagga
    22141 tgagctttga aaaattcgac caaacggtct tggtatttgc taaggcgaag gaagtatgat
    22201 tcttcagaaa cccattcaac ctcatgacct gatggagcaa taccaccagt cacatttcca
    22261 gcttcatcac ggaaaacttc tgccagctgg ctttctgtaa agaattcttc gtctgatact
    22321 gaataccaac cagagtattc acccaagtag atatcatctt gagcaagtaa gcgttcaaag
    22381 acctgtgcga caactttttc atggtagtca tcggttgtac ggataaattt atcgtatgag
    22441 atatctagta attgccagag ttctttaact ccaaccgcca ttccatcaac ataggcttga
    22501 ggtgtaatac cagcttcttc cgctttctgc tggattttct gaccatgttc atcaagacct
    22561 gtcagataaa atacatcgta gcccatcagg cgtttgtaac gtgctaggac atcacatgcg
    22621 atagttgtgt aggcagaacc gatatgaagt ttcccagatg gatagtaaat cggcgttgta
    22681 atataaaaat ttttttcaga cataattttt cctttccagg caaatgaaac ctgtttttct
    22741 aacacttcat tatatcacat ttttaatgaa tttcgatagg gaaatccata caaaaacaag
    22801 atagacgagt gtccatcttg ttgatctcat tcataacgaa gggcttcaat tggatcaagt
    22861 ttcgatgcct tgttggctgg caagactcca aaaatcatac caacactagc cgaaactgca
    22921 agactaaata gggcaactgg gattgatact ccaacttcta taccttctat taaaccttgc
    22981 agtaacaaac ctgctaaggc agttaaacca cttgcaattg tcaagccaat taagccacct
    23041 aacaaggtca aaatcatgga ttcaatcaaa aactgaatta aaatattggc acgtgttgca
    23101 cccaaagcct tacgaagacc aatctcacga gtgcgctctg tcaccgaaac cagcatgatg
    23161 ttcatgacac cagttcctcc aacaaagaga gaaatccctg cgatggaact aataatcgtc
    23221 gtcataaaac taaacgattg ttgaatttct gcaaatacaa cggactcatc tgccacctgg
    23281 tattctccct gttgtaagcc tgcaagctct gtcatttttc gtgccagttc tggacccaga
    23341 gttggggtta aactggtatc attcactcga aagacaatat tagctatttc atctacatta
    23401 aaattcgcag caagggagat attggtagta ataggcaagc caccaaaccc atatattttt
    23461 gatcttttag cctccggact agtataaacc ccaatgaccc ggtaactaaa tccattgact
    23521 tctacaacct tgttaatagc ctcttgagga gattcaaata aactaatgga caattcctca
    23581 tctagcaaaa tgacacttgc aaactctttg aaatcttgct ctctcagact acgacctgca
    23641 ataatttcat tcttaacagc gtccatgtaa gttctgtttc cacctgtcaa attagcattc
    23701 tcaacctttt tatcttgata ggtcaagatg gcattcgttg aattggttac atagtaacta
    23761 tccactccct tcagtttagc tgcctcttga acccaggatt cttgcggttt tggcggttca
    23821 acaggaactt cctcttcctt tccagaaacc gtaaaagctg attgtttctg agtaaaagac
    23881 ccatctttac tttttttagg agagaaaaag acgctaatat ttttctgaga tttagtcata
    23941 tctttattga cttgacgaga tagggaatca cccaaagcca taatcacaac aactgatgaa
    24001 acaccgataa taatcccaat catagtaagc aaagaacgca tcttgtgagc catgatagat
    24061 gaaaaggcaa atttcagatt ctgcatctta gttttcctcc tttcctaact gagcactgtc
    24121 agacgaaatg accccatccc gaatgacaat ctgacgtttg gcataggcag caatctcagg
    24181 ctcatgcgtt accatgataa tggtttttcc ttctttattc aaatcaacca ataattgcat
    24241 aatttggtta cctgttttgg tatccaaggc tcctgtcggt tcatccgcta ggataataga
    24301 aggattgttt accaaggcac gcgcaatggc tacacgttgc ttttgaccac cagataattc
    24361 tgaaggtaaa tggtgactac gttctgtcaa ttcaaccttg tctaaatatt cctcagccaa
    24421 cttgcgacgt tttgaagacg aaactcctgc gtaaatcaag ggcaattcta cattttgcag
    24481 agcattgagc ttcgatagaa gaaagaactg ctgaaagaca aaaccgattt gttggttacg
    24541 gaccttagcc agttgttttt caccaagccc agccacttct tgaccttcaa gataatattc
    24601 tccactggtt ggtgtatcca acatgccaat cgtattcatc agagtggact taccagaccc
    24661 agatggtccc atgatggcta caaattcacc ctcattcact tctagattga tatttttgag
    24721 aacctgcaat tcttggtcac cattacggta gcttctgaag atatttttta gactaattag
    24781 ttgcttcatc agccttcacc tcttttcctt cttccaagga agatgttgga ttactgatga
    24841 ccttagcacc gttcgttaaa ccagaagtga tttcttgatt ttctgcgtca gcatttccca
    24901 atgaaacctc aactttttta gccttttgtt gttcatccac aatccagaca taatttttac
    24961 tatcatccat tactagactg ctaacaggaa caagaatagc cttagttttg cttttaacct
    25021 caatgttgac agaaaaacct tgtttcaaat caccaacctc gcctgtcaca tcaatagtat
    25081 aagggtattt agaacctgta ttattcccgg ctgctggact agctgcttca ccattgtttt
    25141 taggatagtc agaaatatag cttaatttcc cagtccattt tttatcagga tacactttag
    25201 aagtaaagct tacttcttga cctacagaaa ggttggctag attgtactca gacaattctc
    25261 ccttgacttg taaattttca ttgctgacaa tatgaaccat aacttggctc gcccctgttg
    25321 gagatttaga aacattgcta ttgacttcga ctacagttcc ctctagggta ctgagaacag
    25381 ttgttgcatc caattgactt tgagccttac ttaattgcgc cgcagcatct gcacgcgcat
    25441 cacgggcatc acccaattga gcgtcaatag aagcaacaga atttccagcc actggagttg
    25501 ggctttgcac cgttgcatct tctcctccta ctggcgctgg taactgtgga gccggagctg
    25561 aagcggcttc atttcgtgct tgattgagtt cattgatatg acgatctgcc ttagctactg
    25621 ctcgactagc tgaatcatag gccgcctgcg cttctgaact actgtacttg actaaagcct
    25681 gcccttcgct gaccttatcg cccacagaaa caaggatttc atctaaatca cccttactag
    25741 catcaaaata aacatattgt tcattttttg ctgttactgt ccctgacaat aaaacagagg
    25801 atgccacgct tccttccttg gcaacaacaa gatgagtagg ctcatctttt agagcagtct
    25861 gagaaggttg tctaaagagt aaaatccccc cggcacccaa tacaactaca ctcgcagcac
    25921 cgattgctgc atacagttgc cactttttag ctttaccatt ctttttcttc ataatgaaac
    25981 tccttttctt ttttacaata ctttgctatt ataccaaatt tccctccagc aaacaatgca
    26041 gttcagatgg aaataatggc agttcaggat taaacaatcg ttcggaattt tgcttttcta
    26101 ttgtactaga tatataatac actatcttga cttatttggc aagctttttt gcaatttttt
    26161 gtgcgtagca gatttttgca atcaaatcaa aaaaagaata aaatataact atcaaaaaag
    26221 acactctact atttaaaaga ttacaaagga gttttcaatg agagaatatg atatcattgc
    26281 tatcggtgga ggtagcggag gaatcgctac catgaaccgt gctggtgaac atggagccaa
    26341 agcagccgtt attgaggaaa agaaattagg tggaacctgt gtcaacgtcg gttgtgttcc
    26401 taaaaaaatc atgtggtacg gggcgcaaat cgctgagact ttccatcaat ttggagaaga
    26461 ctacggcttt aagactactg atcttaactt taactttgca accctacgtc gcaatcgtga
    26521 agcctacatt gatcgcgctc gttcttctta tgatggtagt tttaaacgca acggtgtaga
    26581 cttgattgaa ggtcatgctg aatttgtaga ttctcatact gtaagcgtaa atggtgaact
    26641 gattcgtgct aaacatatcg tgattgctac aggtgcccat ccaagtattc ctaatattcc
    26701 tggtgctgag ctaggtggct cttctgatga tgtatttgcc tgggaagaac ttccagagtc
    26761 aattgccatt ctaggcgctg gttatatcgc cgttgaatta gctggcgtac tccacacttt
    26821 tggtgtcaag acagatctct ttgttcgccg cgatcgtcct ttacgtggtt ttgattccta
    26881 catcgttgaa ggtttggtca aggaaatgga aagaacaaac ttaccacttc acactcacaa
    26941 agtccctgtc aagttagaaa aaactactga cggcattacc attcatttcg aagatggtac
    27001 tagtcacaca gctagccaag ttatctgggc tacaggtcgc cgtccaaacg ttaagggctt
    27061 gcaacttgaa aaagctggag tgactctgaa cgaacgtggc tttatccaag tggatgaata
    27121 ccaaaatact gttgttgagg gaatctatgc tctaggtgat gtaacgggcg agaaagaact
    27181 gactccagtt gcaatcaagg ccggacgtac cctatctgaa cgtctcttta acggaaaaac
    27241 tactgcaaaa atggattact caactattcc aactgttgtc ttttcacacc ctgctatcgg
    27301 aactgttggt ttgacagaag agcaagctat taaagaatac ggtcaagacc aaatcaaggt
    27361 ttataaatca agctttgcat ctatgtactc tgcttgcact cgcaaccgtc aagaatcccg
    27421 tttcaaactc ataacagctg gttcagaaga aaaagttgtc ggacttcatg gaattggcta
    27481 cggcgttgat gaaatgattc agggatttgc cgttgctatc aaaatgggag caaccaaggc
    27541 tgactttgat gcaactgtgg caattcaccc aactgcatct gaagaatttg taaccatgcg
    27601 ttaatcgaaa caaaagaagc tgatataaat atgtctgctt cttttttaat gattttcgaa
    27661 tcaacaaaaa aattatccaa cttgactagt cacgaaaatc ggctgttaca gtttttgtta
    27721 ccttgttttt aaaaaatggt tgacatcgac tcctatatga gtataatagt tactatacat
    27781 tagaaaagag gttaactatt ttgaaaaaag ctcacgttta tgctatccct gctattgggg
    27841 ctgctctcat tgctgttttg gcacaaatca gtcttccaat tggacctgtt cccttcactc
    27901 tgcaaaactt tgcaatcggc ttgattgcta ctgtctttag accgagagag gctgtacttt
    27961 ctgctggact ctatcttctt ctaggtgcta tcggtcttcc tgtctttgca ggaggtggag
    28021 ctggttttca ggctttagtt ggccctactg caggctatct ttggttttat ctcgtttact
    28081 ctggacttac ttcctctcta accaacagca agagtggtgt tgttaagatt tttcttgcaa
    28141 acctcttggg tgatgccctt gtctttgtcg gcgggattct cagcttgcat ttcctagctg
    28201 gaatggcatt tgaaaaagct cttgttgtgg gggttcttcc ctttatcatt ccagaccttg
    28261 gcaaacttct ggctattagt tttattagcc gtcccctact tcaacgcctt aaaaatcagg
    28321 cttactttac taactaaaaa aggataccga gttatcatga ctcaatatcc ttttttttat
    28381 tttgaaaact tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc
    28441 tcaaaacact gttttgaggt tgtagatgaa actgacgaag tcagtaacca tacggcaagg
    28501 caaagctgat gcggtttgaa gagattttcg aagagtatta gttgccttta aaggcatcca
    28561 ccatcgtttg aaattcttca tttgagagag taatcccttt gcccatttta gtatggtctg
    28621 gactccaagc acgaatatca aactttgcag gggcaccatt aaagctcaca cggttaattt
    28681 ccttggtcca acctttttcg ttttcagaaa gagtcaacaa gtgctcttcg atttcaaatg
    28741 taaattctgc cattttcttc tcctttttta gtttcattag tttattcgta aaatcttgta
    28801 gattttagga aaattttata taatattgat ataaaagaag ggaggccaat atgagacata
    28861 aattccagca agttctagat aaaatacatg attttttaaa tggacatgac caacctgacc
    28921 agactgaaac caactccctt acagccacta ttgaagaggc tatccagaaa caaaccgctg
    28981 ttcaccttat cttgtctgag acaagcttta caggtgatat catcaaatat gatcagcaag
    29041 gccagcaaat tatcgtgaaa aattttgcca aaaatgtgag ccggattatc cgtataagcg
    29101 atattcaacg cctgcgattt gtcccctcaa ctgtccaaac agcccaaaaa aatagattta
    29161 agaaagagtg agatgtagtt gcttcatccc actctttttt cttagcgaat ttgttcaaaa
    29221 tgtaaatgaa ctgcgatatg atctccataa ccacttcttt ccaagtcacg ttgtaaacga
    29281 taggaaatgt agtgttctgc aatggtaatg taacctgcgc ccaataaacg atgttcaacc
    29341 atagattgaa tcatactgat agtcgcacgt tccaccttgg cttcttgtaa atccaaaact
    29401 accttcttag tgacttgagc aagattttga cgcaaatcat ctgtcaaaac ataaacagtt
    29461 tgggctgcct tcaagatggc ttggtaaatc ttatctggat taaattcagc aatttcgcca
    29521 ttacgtttga ttacttgcat aggtttctcc tttattcttt gttttctttg atttctgcca
    29581 gcattttttc ttcttctact gtcagttgat aatgttcaag taaatccggt ctgcgctcgt
    29641 aggttttctt taaactctcg tacaatcgcc actgacgaat cttttcatgg tggccactca
    29701 tcaatacatc tggcacgacc atgcctcgat aatcataggg acgtgtgtac tgaggatatt
    29761 ctaaaagacc tgaagaaaaa ctatcatctt ggtggctaga ctccttgcca atcacttctg
    29821 gaatcaggcg aactgtagca tcaatcatgg tcatagctgc caattctcca ccagtgagga
    29881 catagtcgcc tagggaaatc tcatctgtta ccaaggtctt aatgcgctca tcataaccct
    29941 catagtgccc acagataaag attagctctt cctcttgagc caaatcttca gcataagcct
    30001 gatcaaactg ctttccagca ggatcgagga gaataacgcg cggatttttc ttttcaatag
    30061 catcaaagga atcgaaaata ggttgtgctc tgagcaacat gccctgaccg cctccgtagg
    30121 gctcatcatc tacatgacgg gccttttcag cattttctcg aaaattatga tactggatat
    30181 ccaagagccc tttttctcga gcctttccaa cgattgagtg ctccagtgga gaaaacatct
    30241 ctggaaagag ggttaaaata tcaatcttca tcgtctaacc cttctaagat ttccacatcg
    30301 acccgtttat ttggaatatc aacattgaga accactggtg ggatataagg taaaagcaaa
    30361 tcacgtttgc cttttcgttt gaccacccag acatcattag cacctggttg caggatttcc
    30421 ttgatggttc caaccaagct atcaccctca tagacttcca aaccgataat ctcgtgatag
    30481 taaaattcac catcgtctag gtcattcaaa tcttcctcag cgaccttgag actgtatccc
    30541 ttgtactttt cgatagtatt gatatggtac atatctttga atttaataat gtcaaagttc
    30601 ttctgtttac ggtggctagc gatggtcact gtttggacaa actgatcttt ttcatcaaac
    30661 aaagtcagct cagctccttt tttaaaccgt tcttctgcaa aatccgtcac agacaatact
    30721 cgcatctcac cctgtaatcc ctgcgtatta acgattttcc caacattaaa gtagttcatc
    30781 ttgtctcctg taatctcctt tttccatctt attctgacaa ttctcgaata atagccgcaa
    30841 ttttttccga ttctgaccat tgtaaataat ggtgattcct tcctaaaatg agtttagtat
    30901 tggaagtcca atattctgat tctctgtact ctttttctct ataagtctga caaaaaataa
    30961 atacagggac atgagattct atcgacaaat tctcaaaatc ttcctcagta atctctccag
    31021 atatctgaaa ttctggatct tgattttcca actctaagcc tttttcttgc attaattccc
    31081 agattttttt attcgtttca ggactaaatg ttgcttgagt taagttctta aaataaagtt
    31141 caggaccaca ctcgtcaatc agcctcatct gctcttccat ttctggataa ggattttctg
    31201 aaaaatcagc aaacatgact tttttagttg tcggttcaat tgctactaaa gtctgacact
    31261 taattggttt ctcgagtaat ttgcaagcta aaattccact ccaactatgt gcacaaagta
    31321 tatattcaga aattcctaat tcttcaagta cttcataaac cgcatctgca agattatcta
    31381 gattttttcc agcttggtca tgaatcggac tcctacctgt gttcggaaaa tcaattgtca
    31441 aataaccaat tgtaggagga ggtttttcaa gtataagtga aaaattttca taacttggta
    31501 gcaaacctgc tccgtttaga caaactagca ctttcttttg ctttttataa gtaacagaga
    31561 ggctaccaat ttctgtagat acttcaaacc tcttcataaa gaaatccact cattctatat
    31621 aatgaattat taaaaatcct tatcctttat tttatcacgt tccaaagatt ttctcaagtt
    31681 ggaggaaggg gacaatatct ctactttccc ttcaagaatc cttccaaatc atgtttatgt
    31741 tggtaattaa cggctgcggt tttgtctttc tcaaagatgg ttctagttag gtcaatatgc
    31801 ttgatagcag caatagcgac ggtgtagtga atgatattag ccagttctat ggcaggttcc
    31861 tcgttcgaat cctatccctt cttttcgacc agagcgccta ttcaaaacct cggctacttc
    31921 tccgacttcc tccactaact tcataaagag accttcatca gtccgagact gctgttaatg
    31981 ttcgagtaag tagtcttgga attgcctaaa cgttcaatct tttatagtat attgaaacta
    32041 gaatagtaca cctttacttc taaaacattg ttagaaatcg atttgactgt cctgatcgat
    32101 ttgtcctgtt attatttcat tttactatat cttctattcc acacaaaaaa gcgagacatc
    32161 cgtcccgccc ttcttatttt tcgtcaataa cgattcttac ttttttgtat tcagttggga
    32221 cagagtagac aatcgttctt atcgcagaaa tagtgcgacc cttacgaccg attacacgac
    32281 ccacatcgct ttgatcaaga ttcaaatgat attccaaaaa ttctggtgta tcctcaatct
    32341 tgatagttaa ggcatctggt tgtgaaatta agggtttcac aatcgcaata atgagatttt
    32401 caatcgtatc catctgtcaa cctactttaa acttattttg agaatttaga atcgtggaat
    32461 tttttcaata cgccttcttt tgaaaggatg ttacgtactg tgtctgaagg ttgagctcca
    32521 ttagccaacc atgcaagaac gcggtcttct ttcaaagtta cttggttttc agcaacaagt
    32581 gggttgtaag ttccaactgt ttcgatgaaa cgtccgtcac gtggtgaacg tgaatctgct
    32641 acgttgatac ggtagaaagg ttttttctta gaacccatac gagtcaaacg gattttaact
    32701 gccattttta aagtctcatt tctttaattt tttatttcgg tgaaatagct gagctattta
    32761 gcacatgttc tattatagca gatttctggc aggtgtcaag aaaaaaactt gacagactat
    32821 aaatttctta aagcatttag aaatttgtta gaataggaaa taaatgtttg aaagatgcta
    32881 tccgtgaata ctattttaga aaaattttat aaagaacacc aagtcaaacc cttcatatcg
    32941 cctgaacgag atttggatac ttggcttcta agtcccaaac ccgttcccaa gcgaaatatg
    33001 gacttattag cagatgattc actagcagga gatatcattt tactatggag aatccagttt
    33061 ggtactttta ccactgaaac atgattttaa aaactcccat tcgaatatga ttcaaaattc
    33121 gcttagaatc aatgttttcc cacttt
//
LOCUS       DAVLRT010000015        32323 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLRT010000015 DAVLRT010000000
VERSION     DAVLRT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.015"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            142..1131
                     /gene="manX"
                     /locus_tag="VXM85_000706"
     CDS             142..1131
                     /gene="manX"
                     /locus_tag="VXM85_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1345329.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQAAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1159..1962
                     /locus_tag="VXM85_000707"
     CDS             1159..1962
                     /locus_tag="VXM85_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1345330.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1986..2897
                     /locus_tag="VXM85_000708"
     CDS             1986..2897
                     /locus_tag="VXM85_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1345331.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2953..4287)
                     /gene="pepC"
                     /locus_tag="VXM85_000709"
     CDS             complement(2953..4287)
                     /gene="pepC"
                     /locus_tag="VXM85_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1345332.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(4504..5226)
                     /locus_tag="VXM85_000710"
     CDS             complement(4504..5226)
                     /locus_tag="VXM85_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1345333.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DINTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     KPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(5539..5769)
                     /locus_tag="VXM85_000711"
     CDS             complement(5539..5769)
                     /locus_tag="VXM85_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1345334.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            complement(5779..7020)
                     /locus_tag="VXM85_000712"
     CDS             complement(5779..7020)
                     /locus_tag="VXM85_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1345335.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDLLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVIFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSLVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(7042..7194)
                     /locus_tag="VXM85_000713"
     CDS             complement(7042..7194)
                     /locus_tag="VXM85_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345336.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(7215..7493)
                     /locus_tag="VXM85_000714"
     CDS             complement(7215..7493)
                     /locus_tag="VXM85_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1345337.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(7486..7749)
                     /locus_tag="VXM85_000715"
     CDS             complement(7486..7749)
                     /locus_tag="VXM85_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1345338.1"
                     /translation="MSKMSMSIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(7851..12242)
                     /locus_tag="VXM85_000716"
     CDS             complement(7851..12242)
                     /locus_tag="VXM85_000716"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1345339.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VDKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     GAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEAIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(12346..14427)
                     /gene="fusA"
                     /locus_tag="VXM85_000717"
     CDS             complement(12346..14427)
                     /gene="fusA"
                     /locus_tag="VXM85_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1345340.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(14852..15322)
                     /gene="rpsG"
                     /locus_tag="VXM85_000718"
     CDS             complement(14852..15322)
                     /gene="rpsG"
                     /locus_tag="VXM85_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1345341.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(15342..15755)
                     /gene="rpsL"
                     /locus_tag="VXM85_000719"
     CDS             complement(15342..15755)
                     /gene="rpsL"
                     /locus_tag="VXM85_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1345342.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16172..19969
                     /gene="spuA"
                     /locus_tag="VXM85_000720"
     CDS             16172..19969
                     /gene="spuA"
                     /locus_tag="VXM85_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1345343.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWHLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDLAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDRQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            complement(20069..21118)
                     /locus_tag="VXM85_000721"
     CDS             complement(20069..21118)
                     /locus_tag="VXM85_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1345344.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SDGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHIPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            21175..21312
                     /locus_tag="VXM85_000722"
     CDS             21175..21312
                     /locus_tag="VXM85_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345345.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTNEVSNHTYGKATLTWFEEI
                     F"
     gene            complement(21389..23197)
                     /gene="glmS"
                     /locus_tag="VXM85_000723"
     CDS             complement(21389..23197)
                     /gene="glmS"
                     /locus_tag="VXM85_000723"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1345346.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYFEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(23390..24769)
                     /gene="bglA"
                     /locus_tag="VXM85_000724"
     CDS             complement(23390..24769)
                     /gene="bglA"
                     /locus_tag="VXM85_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1345347.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(24869..26722)
                     /locus_tag="VXM85_000725"
     CDS             complement(24869..26722)
                     /locus_tag="VXM85_000725"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1345348.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(26735..27994)
                     /gene="rseP"
                     /locus_tag="VXM85_000726"
     CDS             complement(26735..27994)
                     /gene="rseP"
                     /locus_tag="VXM85_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1345349.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTVLPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGGKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(28016..28819)
                     /locus_tag="VXM85_000727"
     CDS             complement(28016..28819)
                     /locus_tag="VXM85_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1345350.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(28828..29586)
                     /locus_tag="VXM85_000728"
     CDS             complement(28828..29586)
                     /locus_tag="VXM85_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1345351.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(29817..30281)
                     /locus_tag="VXM85_000729"
     CDS             complement(29817..30281)
                     /locus_tag="VXM85_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1345352.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(30370..31341)
                     /gene="ruvB"
                     /locus_tag="VXM85_000730"
     CDS             complement(30370..31341)
                     /gene="ruvB"
                     /locus_tag="VXM85_000730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1345353.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            31502..31594
                     /locus_tag="VXM85_000731"
     CDS             31502..31594
                     /locus_tag="VXM85_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1345354.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         9358 a         7530 c         6238 g         9197 t
ORIGIN      
        1 tcaacatatc aacatacaaa aataaaaatt ttttaatttt ttatgataaa tagttgcttt
       61 ttttatgaaa acggtttata cttaaacagt cttaaagttt tcttaaatga aaactaaaac
      121 aaaaaaggag gaatgacaat aatgagtatc ggaatcatta ttgcgagcca cggcgaattt
      181 gctgcgggta ttcatcagtc aggatctatg atctttggtg aacaagaaaa ggttcaagtt
      241 gtgaccttta tgccaaatga aggtcctgat gatctatacg ctaagtttaa taacgctgtt
      301 gctgcatttg acgcagaaga tgaggttcta gttttggctg acctttggag tggttctcca
      361 tttaaccaag ctagtcgcgt gatgggagaa aatcctgagc gtaagtttgc catcatcaca
      421 ggacttaact taccgatgtt gattcaagcc tacacagagc gcctcatgga cgctgctgca
      481 ggtgtagaaa aagtcgctgc taatatcatt aaagaagcca aggatggcat caaagctctt
      541 ccagaagagc taaatccagt tgaagaagtt gcaagcgctg cagctgctcc agttgcccaa
      601 gctgctatcc cagaaggaac tgttatcgga gacggtaaat tgaaaatcaa tcttgcccgt
      661 cttgacacac gtctacttca cggtcaggtt gcaactgctt ggactccaga ttcaaaagca
      721 aatcgtatca tcgttgcttc agataacgtg gctaaagacg accttcgtaa agaattgatt
      781 aaacaagcag ctccaggtaa tgtcaaggct aacgtggttc caattcaaaa actgattgag
      841 atttcaaaag acccacgttt tggagaaaca catgccctta tcttgtttga aacacctcaa
      901 gatgcccttc gtgccatcga aggcggcgtg ccaatcaaga ctcttaacgt tggttctatg
      961 gctcactcaa caggtaaaac attggtcaat accgttttgt ctatggacaa agaagatgtt
     1021 gcgacatttg aaaaaatgcg tgacttgggt gttgaatttg atgtccgtaa agtaccaaat
     1081 gattctaaaa aagatttgtt tgacttgatt aacaaagcca atgtcaaata agccattatt
     1141 tatgaaagga ttttaaacat gtctattatt tctatggttt tagtagtcgt tgtagccttc
     1201 tttgcaggtc ttgaaggcat cctcgaccag ttccaatttc accaaccact tgtagcctgt
     1261 acccttattg ggcttgtaac aggtcacttg gaagcaggga ttatcctcgg tggatcgctt
     1321 caaatgattg cccttggttg gtcaaatatc ggtgctgcta tcgctcctga tgctgcactt
     1381 gcttctgtcg ctgctgccat tatcatggtt cttggtggtg actttaccaa gactggtatc
     1441 ggtgttgccc aagcggttgc tatccctctt gccgtagctg gacttttctt gacaatgatt
     1501 gttcgtacaa tttcagttgg tttggttcat actgcagatg ctgccgctaa aaaaggtgac
     1561 ttcggcgctg tggagcgtgc gcatttcatt gcgctactct tccaaggact tcgtatcgcg
     1621 cttcctgcag ctcttctcct tatggtacca actgaaactg tacaaagtat ccttagtgcc
     1681 atgccagact ggctcaaaga tggtatggct atcggtggtg gtatggtcgt tgccgttggt
     1741 tacgccatgg ttatcaacat gatggcaact cgtgaagtat ggccattctt cgctcttggt
     1801 ttcgttctcg ctgctgtgtc agatattact ctaatcggat tcggtgctat cggtgttgct
     1861 atcgctctta tctaccttca cctttctaaa actggtggaa atggtggcgg aggagccgca
     1921 acttctaacg acccaatcgg cgatatccta gaagactact aagataagaa aggactgaaa
     1981 acatcatgac tgaaaaactt caattaacta aatcagatcg taaaaaagtt tggtggcgtt
     2041 caaccttctt acaagggtct tggaactttg aacggatgca aaacttgggc tgggcttata
     2101 cactcattcc agctatcaaa aaactctata ctaaaaaaga agatcaaatc gctgctcttg
     2161 agcgtcacct tgagttcttc aacactcatc catacgtagc tgctccagtc atgggggtta
     2221 ctcttgcgct tgaagaagaa cgtgctaacg gtgtggaaat cgatgacgct gctatccaag
     2281 gggtcaaaat cggtatgatg ggacctcttg ctggtatcgg tgacccagta ttctggttta
     2341 cagtacgccc aatccttgga tctctcggtg cttcacttgc ccttactggc aatatcttgg
     2401 gtccactcct cttctttgtt gcatggaact tgattcgtat gtcattcttg tggtatgttc
     2461 aagagattgg atacaaggct ggatcagaaa tcactaaaga tatgtctggt ggtatccttc
     2521 aagatatcac taaaggagct tctatccttg ggatgttcat tcttgctgtc cttgttcaac
     2581 gctgggtaaa tattaaattt gctttcgatg tttctaaagt tcaactagat gaaaaggctt
     2641 atatccattg ggataaattg ccagaagggt ctaaaggtat ccaagaagca ttcgcacaag
     2701 taggacaagg attgtctcaa actcctgaaa aagttactac tttccaacaa aacttggata
     2761 tgttgattcc tggattatca ggactactcc ttactttact ttgcatgtac ttacttaaga
     2821 aaaaagtatc tccaatcact attatccttg ccctcttcgc agtgggtatt gtggcacatg
     2881 ttcttcacat catgtaatca agcaactaaa aaggaaccag gttctaaaat ctgattcctt
     2941 ttttctatgc ttttattcag ccaaggctcc cattggatcc catggtgcaa gtacaattgg
     3001 ttctgctcca taggcagctt gttcttctgc tgtcagcaat tccttacgaa caacgatttg
     3061 gtatgtgtat tcgtccatcc aagcgtctga ggcaacaaag taaccatctg taccgacctt
     3121 gtctccccat gagttttcaa ccttccactt ggttgattta ccattttcgt ccaagtcaac
     3181 acctgtcaag accatggcgt gggtcatcaa gctttcgcta tagtccaaac gtccagcctt
     3241 gtcttgagta agtttaatgt ccatgcttga ttcaaagtca taaacatctg tcgcaaggat
     3301 gccagcctta cggttactga gctggccgac atcagaacca aaccaaacag tctcacctgc
     3361 ttgcatttgg gcaatcgcca attctttcaa gcgctccatt ggaacgttga tgtaacgaac
     3421 tgcacggcta ccaaccacat tccccaacat ctcaactgtg taagattttc cgtaaggttt
     3481 atcagcagtt ggagcattga taacagaaac gtagtcttct aaaggaagat tgacatattt
     3541 cttgtaaaac tcttgtggtg tgattccttt ttcacttttg tagttgttat ctttatcgcg
     3601 ataagcaaag tcaaacttgc gtggtggaag tcctaatgac atagcaagaa agttaaagat
     3661 ttcttgcaag aggtcttctt tcttagcttg aacagtcgct tgatctgcac cagaaacaag
     3721 caagtcacgc aagatttgag catcttgacg aagcaattta ttaaggatcg catttagctc
     3781 acgactgctg ctagatgaaa cagactcagg ataaactgac ttaggcacga caccgtattt
     3841 ttcaaagagg gaaacgacca tatcccattg accaccatct tgttgaggtg tttggagtaa
     3901 gaagctaacc ttgcggctag tcaattcttg gtctgaagtc gcaatgactt gctccaagaa
     3961 ccagtttgat ttctcatact tatcccagaa gaaagtgtgg gcttgtgaca actcaaagtt
     4021 ctccaatttg tattgcgaga tgagtttgtg gcggaaggtg ttgagagccg caaacatcca
     4081 gcaacgacca gacgctttct ggttagtgac cttgtccttg gttaaatcca atgagaaaac
     4141 aggtgtgttg tctacatggc tttggcgacg ttccagagct gcaaaaattc cgttgtggct
     4201 agcagcattt tcaatcgctt ggtattttac atttgcttca tagttggcaa atagtttatc
     4261 agtaaatgat tcttgaatcg cgttcataga ttcctccttt tattctacag tctattataa
     4321 ctcttttcac aaaggaagca actaaaaaac ataaaaggta aggacattct ccctcacctt
     4381 tttcgaatat atactcttcg aaaatctctt caaaccacgt caacgtcgcc ttgccgtact
     4441 caagtacagc ctgcggctag tttcctagtt tgctctttga ttttcattga gtattaaact
     4501 aaattaaata atattagcgc ggagaatttc taattcttcc ttggtcaagc gacgccattc
     4561 ccctcgttcc aggttctcat ctaatactaa agttcccata gtcaatcgtt gcaagtccac
     4621 cacttccttg ccacagtagc ccaccatacg cttgatctga tgaaacttcc cttctgcaat
     4681 ggtcacacgg atttggcttt gattcttttc tgtatctatg gatacaagct ccagtatagc
     4741 gggtttacag gtaaagtctt tgagaggaat accctcagca aatgtctcca catcttcttg
     4801 ggtcatgatt cccttgactt gtgccagata agtcttgtcc acatgacgct tgggcgaaag
     4861 aagaacatga gccagctgac catcattggt caagagcaaa agaccatgcg tgttaatatc
     4921 caagcgtcct actgggaaaa cttccttact ccgcgccaag tcatccaaca agtccagaac
     4981 ggttctgtgc ttgggatcct cagtcgctga gataactcct ttgggcttgt tcatcatgta
     5041 gtagacaaac tcttcatact ccaacacttg cccatcaaag cgaatctcat ctattttttc
     5101 atcaatctgc aatttagctg atttttcttt tttaccattt acagtcacgc gcccagcctt
     5161 gagcaagttt ttgacctcag tccgacttcc caccgcacag gcaactaaaa atttatctaa
     5221 tctcatagaa ctattatatc atatcaaaag gaggctggta caatgaccaa cctccttttc
     5281 gtttcatact cttcaaaaat ctcttcaaac cgcgtcaacg tcgccttgcc gtatatatgt
     5341 tactgacttc gtcagttcta tctgcaacct caaaacagtg tttttagctg acttcgtcag
     5401 ttctatctgc aacctcaaaa cagtgttttt agctgacttc gtcagttcta tctgcaacct
     5461 caaagcagtg ctttgagcaa cctgcggcta gtttcctagt ttgctctttg attttcattg
     5521 agtatcagat ttaggaaatt aacttcctcg tctccaaaaa atagctaaga caatcatggc
     5581 acctaaaaca gctgggataa tagctgttcc tgataaaact ggcccccaag ttccaaagag
     5641 caagtgacct agaaaggctc cgatccaacc gagaaacatt tttccaaaac atcccattcg
     5701 ctctccacga ttggtcatag cacctgctaa aaatcccact aggagaccaa cgaacatact
     5761 tcctaacata ttatctcctt aatttgccca attcccatta cggaaaagag gtacccgcgt
     5821 tccatcctca cgaataccat cgatatccat ttggttagaa ccaatcataa agtctacgtg
     5881 aacatctgaa cggttaagcc ctgcagcttc aagctcctct tcgctcatct ccgctccatc
     5941 aacaaggcta gtcgcatagg ctgcaccgat agccaagtgg tttgacgcat tttcatcgaa
     6001 aagggtgtta aagaaggtaa tgcctgactg agaaattgga cttggatctg gtaccaaggc
     6061 acattcaccc aaggcacgcg cacccgcatt ttcaaagaca aggtctttca taacctgatc
     6121 acccttctca gcagtgatat ctacgatttg tccatcctta aagatcacct taatgccttc
     6181 aatgatattt ccgttgtagc taagcggttt tgtagaagtg acataaccat ctgcacgacg
     6241 gaagtcaggc gctgtgaaga cctcttctgt cggcatattt ggcaagaatc cttcgccctg
     6301 tgcattgaca gcaccagctg attcccaaac gtggttcttt ggcaaaccaa gtgttaaatc
     6361 tgttcctggc gctgtgtagt gaagggctga aaattgctcc ttattaagca tatcggcctt
     6421 gctcttgaga atggctgcat gctcttccca ggccttaaca ggatctgctt cgtagacacg
     6481 gcaagttttg aagatttggt cccaaaggag atcgactgct tcttcgtcgc tcgcagcatt
     6541 tgggaagact ttcttagccc actcaagtcc agcagcggct gctacagtcc agctaacctt
     6601 gttagattga gttgcgattc gcattggctt catggcaagt cccatagctt tagcagaagc
     6661 tgaaagcttg tcagcgtcca ctccgttcaa ggcacctgga tcagatgaac gaactccaag
     6721 acggctagcc ttgttctcca agagatagtt catctcagca atcttgtatt ctggcacatt
     6781 gtccaaacgc tccatcgggg catggaggaa tttctcacgg ttaatcacat catctgtcca
     6841 ctgaacgatg acctcatgcg cacccaaggc ataagcttct ttcacgatta gatgtgccaa
     6901 ttcacgttgc tccacatcaa tagagagagc caaagtgtga ccaggttgca cgttaattcc
     6961 gttcgcaacc aacaatttcg catatttttc tagattttct ttaaaatttg gtaaaaccat
     7021 cctgttttct cttttctatt tttatttttc tttcaaagca gaaaagattc ctgctaaagc
     7081 aatagcacct gacaagagtg ctgtcgataa aagaattgta cgatcagcaa gttctaattg
     7141 atattcaaat accttcttag ccggcttatc ttccgcaaat attcttagtt tcatgagaac
     7201 ccccttaaaa tagattaaaa taaatcactg tgactgcctg tcctcagcaa atttaaaatc
     7261 aattccgact tatctacttt ataaaccaaa agccaatctg gctgggtatg gcattcacga
     7321 actccttgaa aatgcttgga tgccgtcaat gaatgatcac gatatctggc aggatgttct
     7381 ttttcttgaa ccagaaaatt caaaacttct tctaataatt ctgccttcaa accacgcttc
     7441 atagccaact taaaatcttt tttaaactgt ttatgataac gaatcttaag cacgtaagtc
     7501 ctccatcaaa tctgagactg attcaaaaga ctggctcata ttacgatttt ggtctaaatc
     7561 cgttaacact tggagcaact tccgattttc gtctagtcta acatcaaaag gtaatccctg
     7621 atattgaatt gcctgacgaa ggaaaatatt aatagctgtt gtcatatcca ttcccagatt
     7681 actaaacacc tgttgggcct gctccttaac ctcactatcc agacggatgc tcatactcat
     7741 ctttgacata ctctcaccct ctttccatag actattttaa caaaaaagaa agctaatgta
     7801 aatctattgg atatacgtta gcctcttcta atagattatt aagcaatttt ttaaaacaac
     7861 tcatcaaaca aactcaactg gttatcctct ggcatatttc caagaatacc catctcatcc
     7921 atcttttcaa ccaaggttga tgagagtcca ccacgcttgc gtagttctgt tttagagagg
     7981 aattctccct cttcacgcgc ccgcaccagt tgcttggcaa cgttctctcc cagaccatcc
     8041 attgctacaa atggtgggat aagggtatcc ccgtcgatga ggaactctgt cgcctgacta
     8101 cggtagagat ctaatttgcc aaacttgaaa cctcgttccc acatctcatt gacaatctca
     8161 agagttgtat agagatcgat ttccacatta gaggcttcat tgttcttccg tttttcagag
     8221 atttcttcca ttctgcgctt gatggcctcc aagcccgcac ccatggtctt gatatcaaaa
     8281 gccttagcac gaatggagaa gtaagcacag tagtaataaa taggatggtg aaccttgaag
     8341 taagctacac gcaaggccat cataacgtag gctgccgcat gggccttagg gaacatgtac
     8401 ttaattttcc cacaggattc gatataccac tctggcacct tattagcctt catggcttcg
     8461 atatagccat ttctctcctc ttctgaaatc tttagccaca aacccttacg tacccgttcc
     8521 ataatggtaa aggccatctt aggttccaga cccgcatgca tgaggtaaac catgatgtcg
     8581 tcccgacaac cgataacagt cgataggtcc gctattcctt gcttaatcag atcctgagca
     8641 ttccccaacc aaacgtcagt accgtgggac agaccagaca gctgaagcaa ttccgcaaag
     8701 gttgtcggat gggtttcgtc taccattcca cgtacgaaat ttgttccaaa ctctggaatc
     8761 cccaacatac ccgtaggcgt tccaatttgt tcaggtgtta cccctagcac atcagtccca
     8821 gaaaagagtg ccatcacgcc ttcgtcatcc ataggaattt cattagggtc aataccagac
     8881 aaatcctgaa gttttcgaat catggtcgga tcatcatgtc ccagtacatc gagtttgagg
     8941 acgttctcat cgatatcgtg gaagttaaag tgagtggtct gccattcagc cgtgacatca
     9001 tctgctggat actggacagg cgtaaaatcg tagacatcca tgtagttcgg aataacaacg
     9061 attccccccg ggtgttggcc tgttgtccgc ttgacacccg ccgctccttg agcgaggcgt
     9121 tctacttctg catcacgata aaacttgcca taatctcgct cgtaaccctt gacaaatcca
     9181 taggcagtct tggcagctac cgtaccaacc gttcccgcac ggaaggcata ttcttcacca
     9241 aagatatcac gcacatccaa gtgggcgcta ggctgatctt ctcccgagaa gttcaagtca
     9301 atatcaggaa ccttatcccc atcaaagcca aggaaggtct caaacggaat atcctgtccg
     9361 tttttactga gtttgtgacc acagtttgga cagtccttat ggggcatatc aaatcctgaa
     9421 ccgtacgaac catctgtgat aaactcactg tactgacact gaccacagac atagtgagga
     9481 gagagaggat tgacctccgt aatcccaatc atggtcgcaa cgaaactaga tccgacagac
     9541 ccacgagaac caaccaaata accccgttca ttagaacgtt gcaccagcat ctgcgatgcc
     9601 agataaatca cagcaaatcc attccccagt atggatgtta attctttttc aatccgcaaa
     9661 tcaacaatat ctggcagcgg atttccataa atctcaaaag ctttcttata ggtcaactca
     9721 gcaactgttt cttcagcctt gtcgatgaaa ggcgtataca agtcaccctt aacgacttca
     9781 acgggttcaa atatttctgc caaggcatta gtgttttcaa taaccagttt acgagccagt
     9841 tcctctccca aaaaggcaaa ttcatccaac atctcattag tcgttcgaaa atgagccttt
     9901 ggaagtggtg ctggttgggc atgttcacca tgaccgatag ttcgattaat catcgcaccc
     9961 tgtcccaaac tacggacaat aatttcacga taaatctctt cttccggttc gatatagtga
    10021 acatttcccg tagccagaac aggcttgcca aggcgatctc caacctctat caaactcttg
    10081 ataatggtct ggagttcctc catatccttg acctgctctt tggcaatcaa gggcgcatag
    10141 atagccggtg gcatgacctc gataaagtca taatacttgg ccacctcaac cgccccatcc
    10201 acaccttgag aaacgaccac gtcaaaaact tcaccctctg aacaggctga acctaaaatc
    10261 aagccctctc gatgggcatc tagaaccgtt ctcggaatcc gtggcactcc ttcaaaatac
    10321 ttggtattag acaaggaaac cagcttaaag atatttttta gacctacctg attcttgaca
    10381 tagatggtcg catgcttgat ccgagctttt ttgtaagaat ctggactgat tagatcaatg
    10441 ttgagtctag ctaaatcggt cacaccatgt ttttctgcta cctctttgat aaagatgaaa
    10501 agcagacgac cagtcgcttc cgcatcgtaa ttggccatgt ggtgatgttc caaggccaca
    10561 ccaaaacgct tggtcaaagg ccccaaacca tggcgtttat actcaggata gaggtttcta
    10621 gcaaactcca gcgtatcaat aactggctga ctaatctttg gaagaccatg acgctcataa
    10681 ttagcattca taaagccaac gtcaaaggta gcattgtggg caactaggac cgtatccttg
    10741 caaaattctt ggaattcttg caaaacttgt tctagtggtt tggcattttt gacatgatca
    10801 tctgtaattc cagttaactc tgtagtaaag gctgacaagg gatgcccagg attgataaat
    10861 tcatcaaatt cagcaataac attccccttg tacatcttag aggccgcaac ctgaatcaag
    10921 tcattataga tagctgaaag tcccgtcgtt tccacgtcaa agaccacgta ggttgcttct
    10981 gacaagtcca tctccacttc gttatagacg atagggacac ggtcctccac gatattggct
    11041 tccatcccat agatcagctg gattcccgct ttcttagccg ccttatagcc atgtggaaag
    11101 gactggacat tcccatggtc cgtgatagca accgccttgt gtccccactt agcagctgtt
    11161 gcaacaatct cttcgacctc tggcaaagca tccatagtcg acatgttagt atgagcatga
    11221 aactcaaccc gacgctcacc ttctggcatc aaatccttcc gctcatagtg aacaacttcc
    11281 tgcagatcct gtacgttcat agtcaaatcg cgtgtgaagt tattcatctc cacattccct
    11341 cgaactcgga gccaagaatt cttcttgatg agatcaaact tctgggcctc ttcctcgttt
    11401 ttaacccact tttgcataga aaaacttgaa gtgtagtccg tcattttaaa gttgattaaa
    11461 acacgacctg ttctagtcac tttttgctcc acatcaaaaa caaccccttc aaataccaga
    11521 cgattttcct ctgtcgtcac ttcgatcata ggagtaatct ccgccttatc cagcttgggt
    11581 ttagctgcag cttttttcgc ttgaaaatca aagactggtt tctcttccgc tggaggaggt
    11641 gccatctgct ccagttgttc catagcacgg agcgcttcct cattggcagc ttgaacaatc
    11701 tgctcatttt cagcatgaaa ggcctcttcc tgctcttggg tcaggacatc attcttatcg
    11761 acttgacagt taaaagttgg aaaaccaaac ttttcaagtt gtttggctaa attaggaaga
    11821 tgattcttct taaaatgttc cttatcaatc gcctcagatc cttcaataaa tagctgatta
    11881 ccctcagcac gaacttgcaa attttgataa agggacttaa aaccttgact agcacatgga
    11941 ccttcagaga aagcctccct atagtaggac tgcaagagct gatttgaaaa ttcttgagac
    12001 cgagccttaa tttcaaaaac agctttattg cctgtcttag aaaattcttc gctcaaacct
    12061 ttctttaatt ctaaaaagat ttcaatcggt aaaatattag aaaatacgaa atgaaactcc
    12121 cataccttac taattttatg aaccacaact cgctcaatat tggcctgtgc taaagcagga
    12181 gcctgtctca tttcagcagg catccccaat tgattcatca aaatttcaaa actatttgac
    12241 attcattttc ctcacattat tcttctacta ttttaccata tttagaggta ttttctaaag
    12301 acaaaaggaa gccactaagt gacttccttc tagagtgagg acggattagt cttcaccttt
    12361 atttttctta ataatttctt cttgtactga cttaggtaca tcttcgtagt ggtcaaatac
    12421 catcatgaat gtaccacgtc cttgagatgc agaacgaaga actgttgcgt aaccgaacat
    12481 ttcagcaagt ggaacgtaag cacgaacgat ttggctgtta ccgtgtgctt ccataccatc
    12541 tacacgtcca cgacgagcag ttacgtgacc cataacatca ccaaggtttt cttctggaac
    12601 agtgattgtt acaagcatca ttggttcaag gatagctggt tgtgctgatt tagcagcttc
    12661 tttaagggaa agtgaagccg caatcttgaa ggcagtttca gatgagtcga catcgtgata
    12721 tgaaccatca taaagcttag ctttaacgtc aaccattggg taacctgcaa gaacaccgtt
    12781 agccatagat tctaccaaac ctttttcaac cgctgggata aattcacgag gaaccacacc
    12841 accgacgatt gcgttttcga attcgaatcc tttaccttct tcgtttggag taaattcaat
    12901 ccacacatca ccgaattgac ctttaccacc agactgacgt ttgaagaatc cacgtgcttg
    12961 agtagaagcg cggaatgttt cacggtaaga tacttgaggc gcacctacgt tcgcttcaac
    13021 tttgaactca cgacgcatac gatcaacaag gacgtcaagg tgaagttcac ccatacctga
    13081 gataactgtt tcaccagttt caacgtttgt ttcaacgcgg aatgttggat cttcttcagc
    13141 caatttttga agggcgatac ccatcttgtc ttggtcagct ttagattttg gctcaaccat
    13201 caattggata actggttctg gaacgttgat tgactcaagg atgattttag atttttcatc
    13261 tgtcaatgag tcaccagttg tagtatcttt caaaccaacg gcagcagcga tatcacctga
    13321 gtaaacagtg tcgatttctt gacggctgtt agcgtgcatt tgaaggatac gtccgatacg
    13381 ttcacgttta cctttagaag tattcaatac gtatgaacct gattgaagaa cacctgagta
    13441 aacacggaag aatgtcaaac gacctacgaa tgggtcagtc atgatcttga aggcaagagc
    13501 tgcaaatggc tcttcgtcag atgctggacg agtttcttca gcgtctgtat ctgggttaat
    13561 acctttgatt gctgggatgt caagtggact tggaaggtag tcgataaccg catcaagcat
    13621 caattgaaca cctttgtttt tgaaggctga accacacaat actgggaaga attcaacgtt
    13681 gatagtcgct ttacggatac cagctttcaa ttcttcgtta gtgatttctt caccttcgag
    13741 gtatttcatc atcaattctt cgtcagtttc agcaactgct tcaatcaatt tttcacggta
    13801 ttcttgagct tggtcaaggt attcagctgg gatgtcttct tcaaggatat ccgtaccaag
    13861 gtcgttagta tagatttcag ctttcatctt gattaagtca atgataccac ggaagtcatc
    13921 ttcagaaccg attggcaatt ggattgggtg tgcatttgct tgaaggcgat cgtgaagtgt
    13981 gcttacagag taaaggaagt cagcaccgat tttgtccatt ttgttggcaa atacgatacg
    14041 tggaactccg tactcagttg cttgacgcca aactgtttca gtttgaggct caacacctga
    14101 ttgtgagtca agaacggtaa ccgcaccatc caatacacga agagaacgtt gtacttcgat
    14161 tgtgaagtcc acgtgtcctg gtgtgtcgat gatgtttacg cggtggttgt tccattgagc
    14221 tgttgtcgca gcagatgtga tcgtgatacc acgttcttgc tcttgctcca tccagtccat
    14281 ttgtgacgca ccttcgtgag tttcaccgat tttgtggatt ttaccagtgt agtaaagaat
    14341 acgctcagta gttgttgttt taccggcatc gacgtgagcc atgataccga tattacgagt
    14401 tttttcaagt gaaaattcgc gtgccatgag gtttgtttct cctatttatt tttgatttct
    14461 attctattat aacacgattt taataaaaac ggataggcag gacctacccg ttctcaatgt
    14521 tttcatgcta ttgttggttt caacttacga gatggtaagc tgagttatag ctaatactaa
    14581 tcgatttagc taatttgaac ccgggctaaa gttagttagc cgatgtgagc tggaacggga
    14641 tgctgcgtga aaaagataaa actccttgta ttcgtcgaat actgcgtcag tttcctattt
    14701 tcaccttgca tccttaccgt tcctagcatc atgatttagt tgagctcaag ctaaaagtct
    14761 ggagaaaaag atgaatctca ttggttgcaa ccgcaacctg cttcgattcc ctattttctc
    14821 tgggactttc ttaacgcttt cgcatcctat cttaccaacg gaagtgtgcg aatgcacggt
    14881 tagcttcagc catacggtga gtatcttcac gtttcttaac tgctgcacca gtgttgttag
    14941 cagcatccaa gatttctttt gcaagacggt cttgcattgt gtgttcacca cgaagacgag
    15001 cgattgttac caaccaacga agtccaagtg ttgtacgacg ttctggacga acttcaactg
    15061 ggacttggta gttagaacca ccaacacgac gtgcacgtac ttcaagtaca ggcatgatgt
    15121 tttccatagc tgtttcaaat acttcaagtg catcgttgcc agtagcttct ttgatttgct
    15181 caaaagcacc gtaaacgatt gaagcagcag taccacgttt accatcaagc ataacgcggt
    15241 tgataagacg agtaactagt tgtgaattgt atagcggatc tggcaatacg tcacgttttg
    15301 gagctctatt tttacgactc atttctcttt atcccctttc cttatgcttt tggacgttta
    15361 gtaccgtatt tagaacgacc ttgtttacga tcgttaacac ctgcagtatc aagtgcacca
    15421 cggacgatat ggtaacgtac ccctggaagg tcttttacac gtccaccgcg aagaagcacc
    15481 acgctgtgct cttgcaagtt gtgtccgata cctgggatgt aggcagtaac ttcgataagg
    15541 ttgctcaaac gtacacgagc gaatttacga agggctgagt taggtttttt aggtgtcatt
    15601 gttccaacac gagttgcaac accacgtttt tgtggtgaag aaacgtttgt ttgaactttt
    15661 ttatgactat tgtaaccaac gttcaaagct ggtgatttag atttttctac ttttgattta
    15721 cgcggtttgc gaaccaattg gttaattgta ggcatctaca ttctcctgtg tttttttatt
    15781 tttggtgatg atacacttgg tgacagatat catctgtgtg tacttttgca acatttgtca
    15841 gcacgtccct gtacactctt gagagaccaa aagtaaaaag taccgtctat tattgtaaca
    15901 aaattttccc ttgtttgtca agatattttt cttttttatt ggtaatgtta gagatcaatt
    15961 tctgaataaa aatagctggg aacacatcat cttcctaata atacggcttg gcttatgtga
    16021 catttttatc tccgtcattt atactgatat ataaaatagt acagaaaact tctcaaaagt
    16081 aagaattttt ttcacaaatc aaagaaaacg cttgcatatt gttttactac atgctatact
    16141 gatatagaaa tttattgatt ggagaatcat tatgagaaaa acaccatctc acactgagaa
    16201 aaaaatggtc tacagcatcc gttccctcaa aaatggaact ggttctgtcc ttatcggcgc
    16261 aagccttgtc ttgcttgcca tggctacacc aactatctca tccgacgaaa gtacaccaac
    16321 cactaacgaa cccaacaaca gaaatacaac cacccttgcc caacctctta ctgatacagc
    16381 agctggctct ggtaagaacg aaagtgatat ttcttcacct agaaatgcaa acgcttccct
    16441 agagaaaaca gaagaaaaac ctgcaacaga gccaactact tctactagtc cagtaacaac
    16501 tgaaactaag gcagaagagc ccatcgaaga taactacttc cgtatccatg tcaaaaaact
    16561 tcctgaagaa aacaaggatg ctcaaggact atggacttgg gacgatgttg aaaaaccatc
    16621 tgaaaactgg ccaaacggag ctttgtcctt caaggatgcc aagaaagatg actacggcta
    16681 ttacctagat gtcaaattaa agggagaaca agccaagaaa attagcttcc tcatcaacaa
    16741 tacagctgga aaaaatctaa ccggcgataa atctgtagaa aaactagttc caaaaatgaa
    16801 cgaggcttgg ttagaccaag attacaaggt tttctcttac gagccacagc ctgcaggaac
    16861 tgttcgcgtc aactactacc gcacagatgg caactatgac aagaaatctc tctggtactg
    16921 gggagatgtg aaaaatccaa gtagcgctca atggcctgac ggaacagact ttacggctac
    16981 aggcaaatat ggccgctata tcgacattcc tcttaatgaa gccgcaagag aatttggatt
    17041 tttattacta gatgagagca aacaaggaga cgacgtgaaa atccgtaaag aaaattataa
    17101 gttcacagat ttgaaaaatc atagccaaat tttcctaaaa gacgatgatg aatcgattta
    17161 cacaaatcca tactatgtcc atgatatccg tatgacagga gcccaacacg taggcacttc
    17221 tagcattgaa agtagctttt caacacttgt cggtgctaaa aaagaagata tcctcaaaca
    17281 ctccaacatc actaatcacc taggaaacaa ggtaactatt accgatgttg caatcgatga
    17341 agctggtaag aaagtgacct acagcggaga tttctctgac acaaaacatc cttatactgt
    17401 tagctacaat tccgaccaat tcactaccaa aacaagctgg cacctgaaag atgagacata
    17461 cagctatgat ggcaaactgg gagctgacct aaaagaagaa ggaaaacaag ttgatttgac
    17521 cctttggtca ccaagtgctg ataaggtttc tgttgttgtc tacgacaaga atgaccctga
    17581 caaagtagtt ggaactgtcg ctctcgaaaa aggggaaaga ggaacttgga aacaaactct
    17641 agacagcaca aacaaactcg gaatcacaga tttcactggc tactattatc aataccaaat
    17701 cgagcgtcaa ggtaaaactg ttcttgcact cgatccttac gctaaatctc ttgctgcttg
    17761 gaatagcgac gatgccaaga ttgacgatgc ccataaagtg gctaaagccg cctttgtaga
    17821 tccagctaaa ctcggacctc aagacttgac ttatggtaag attcacaatt tcaagactcg
    17881 tgaagacgcc gttatctacg aagctcatgt gcgtgacttc acttcagatc ctgccattgc
    17941 aaaagacttg accaaaccat ttgggacttt tgaagccttc attgaaaaac tagactatct
    18001 caaagacttg ggtgtaaccc atatccagct ccttccagtc ttgtcttact actttgtcaa
    18061 tgaattgaaa aaccatgaac gcttgtctga ctatgcttca agcaacagca actacaactg
    18121 gggatatgac cctcaaaact acttctcctt gactggtatg tactcaagcg atcctaagaa
    18181 tccagaaaaa cgaatcgcag aatttaaaaa cctcatcaac gaaatccaca aacgtggtat
    18241 gggagctatc ctagatgtcg tttataacca cacagccaaa gtcgatatct ttgaagacct
    18301 agagccaaac tactaccact ttatggatgc cgatggcaca cctcgaacta gctttggtgg
    18361 tggacgcttg gggacaaccc accatatgac caaacggctc ctagttgact ctatcaaata
    18421 cctagttgat acctacaaag tggatggctt ccgcttcgat atgatgggag accatgacgc
    18481 cgcttctatc gaagaagctt acaaggctgc acgcgccctc aatccaaacc tcatcatgct
    18541 tggtgaaggt tggagaacct atgccggtga tgaaaacatg cctactaaag ctgctgacca
    18601 agattggatg aaacataccg atactgtcgc tgtcttttca gatgacatcc gtaacaacct
    18661 caaatctggt tatccaaacg aaggtcaacc tgcctttatc acaggtggca agcgtgatgt
    18721 caacaccatc tttaaaaatc tcattgctca accaactaac tttgaagctg acagccctgg
    18781 agatgtcatc caatacatag cagcccatga taacttgacc ctctttgaca tcattgccca
    18841 gtctatcaaa aaagacccaa gcaaggctga gaactatgct gaaatccacc gtcgtttacg
    18901 acttggaaat ctcatggtct tgacagctca aggaactcca tttatccact ccggtcagga
    18961 atatggacgt actaaacaat tccgtgaccc agcctacaag actccagtag cagaggataa
    19021 ggttccaaac aaatctcact tgttgcgtga taaggacggc aacccatttg actatcctta
    19081 cttcatccat gattcttacg attctagcga cgcagtcaac aagtttgact ggactaaggc
    19141 tacagatggt aaagcttatc ctgaaaatgt caagagccgt gactatatga aaggtttgat
    19201 tgcccttcgt caatctacag atgccttccg acttaagagt ctccaagata tcaaagaccg
    19261 tgtccacctc atcactgtcc caggccaaaa tggtgtggaa aaagaggatg tagtgattgg
    19321 ctaccaaatc actgctccaa acggcgatat ctacgcagtc tttgtcaatg cggatgaaaa
    19381 agctcgcgaa tttaatttgg gaactgcctt tgcacatcta agaaatgcgg aagttttggc
    19441 agatgaaaac caagcaggat cagtcggaat tgccaacccg aaaggacttg aatggactga
    19501 aaaaggcttg aaattgaatg cccttacagc tactgttctt cgagtctctc aaaatggaac
    19561 tagccatgag tcaactgcag aagagaaacc agactcaacc ccttccaagc ctgaacatca
    19621 aaatgaagct tctcaccctg cacatcaaga cctagctcca gaagctagac ctgattctac
    19681 taaaccagat gccaaagtag ctgatgcgga aaataaacct agccaagcta cagctgattc
    19741 acaagctgaa caaccagcac aagaagcaca agcatcatct gtaaaagaag cggttcgaaa
    19801 cgaatcggta gaaaactcta gcaaggaaaa tatacctgca accccagata gacaagctga
    19861 acttccaaat acaggaatca aaaacgaaaa caaactccta tttgcaggaa tcagcctcct
    19921 tgcgctcctt ggtctcggtt tcttactaaa aaataaaaaa gagaactaaa ctagccctcc
    19981 tatagaaaaa tcccccaagc attatagctc gggggattaa ttttcgtaca atatttgttg
    20041 tcctaataaa cttgattagg attttttatt aagcctcttt catagcaaaa taagctcgta
    20101 cttttggtgc aacttgtgtt ccgaagagtt caatagctct cagaacctgg tcatgaggca
    20161 tagaaccaag cggtatatga agcatgaagc ggtccaatcc taaatcctct atcatgcgaa
    20221 tcaatttttc ggccacctga tctggattgc caacaaacat ggcgccattt ggccctacct
    20281 gctccaaata ttgctcataa cgcaattcct gccagtgcgg acggtctttg gaaatagcat
    20341 ccaccacttg cttagtcgga tggaaataat ctttcaccgc ctgctcacca tcttccgcaa
    20401 tccaccccca agaatgggct cccactttca agtctttgtc agcatggccc gcttcgcttc
    20461 caatctcacg ataagcctga atcaactttt taaaataacg tggattacca ccaataatag
    20521 catatacaat cggtagacca gcctgagcaa tcttcactgt tgattcgaca tgaccacctg
    20581 tagctatcca caagggcaat ttgtcctgaa ctggacgagg ataaacttct ttaccagcaa
    20641 tcgtttgagt caatcgacct tgccagtcta acttggtctt ttcattgact aactgaagca
    20701 agtctaattt ctcatcaaaa agagagtcgt agtctttcaa gtcataacca aacagaggga
    20761 aagattccgt gaaagagccc cttccagcca taatctccga tcgtccatct gacaaagcat
    20821 cgatagtggc atactgttgg aacaaacgaa tcgggtccat gcttgacaga atgctgactg
    20881 cactggtcaa acggattttc ttggtattga ctgccccagc ggccaggaca atctctgggg
    20941 ctgatactgc aaaatccgcc cgatggtgct cgccaatccc atatacgtcc aaaccaacct
    21001 tgtcagccag ctcaatctct gccaccaact ggcgaatgcg ttcagcatga ctgtaagttt
    21061 gtccagtccc ttcaagctcc gttatttccc caaatgttga aattcccaat tctaccattg
    21121 tgattctcct tgtctatctc tgtacttcaa tttgaaaaat tattctaata ctcaatgaaa
    21181 atcaaagagc aaactaggaa gctagccgca ggttgctcaa aacagtgttt tgaggttgtg
    21241 gatagaacta acgaagtcag taaccatacc tacggcaagg cgacgttgac gtggtttgaa
    21301 gagattttct aagagtataa cacgaatctt gagtacaagc aaccaatttg ctcacaagaa
    21361 aaagcctaga taactagact tttttagctt attctaccgt tactgacttg gcaaggttac
    21421 gtggtttgtc cacatcgagg ccacggtgga gggttgcaaa gtaagcgact aattgcgtcg
    21481 gtacgaccat tgaaattggt gagaggtatg gatgtacggt cgtaaggacg atatcgtcgg
    21541 tatctttggc tacattctct tctgcgatag tgaggacttt ggcaccacgg gctgcgacct
    21601 cttggatatt tccacgagta tggttggcaa gaactggatc tgacaagaga gccaaaacag
    21661 gcgttccttc ttcaatcaag gcaatggttc cgtgcttgag ttctcctgcc gcaaaacctt
    21721 cacactggat ataagaaatc tctttgagtt tgagacttgc ttccatggct acgtagtaat
    21781 cttgaccacg tccgatgtaa aaggcgttac gagttgtttc aagaagttca cgaaccttgg
    21841 cttcaatggt ttctttctct gaaagagttg attcgataga ctgagctacg attgacaatt
    21901 catgaaccag gtcaaaggct tgcgctttag cattaccatt tgcttctccg actgcttttg
    21961 caaggaaggc aagggctgcg atttgcgctg tataggcttt agttgatgcc acggcaattt
    22021 caggacctgc gtgaaggagc atggtatagt tggcttcacg tgagagggtt gaacctggaa
    22081 cgtttgtcac tgttaagctt ggaattccca tttcattagc cttgaccaaa acttgacgac
    22141 tatccgctgt ttcaccagat tggctgataa agatgaagag tggtttcttg ctgagaagtg
    22201 gcataccgta gccccactca gatgagattc caagttcaac tggtgtatct gtcaattctt
    22261 ccaacatttt cttagaagca aatcctgcat ggtaagatgt tccagctgca aggatgtaga
    22321 tgcggtctgc gtcttgaaca gccttaatga tatctgggtc tacgacaact tgaccagcct
    22381 catctgtgta ggcttggatg agtttccgca taacagttgg ttgctcgtca atttccttga
    22441 gcatatagta agggtaagtt cccttaccga tatctgacaa gtcaagttca gcagtgtagc
    22501 tagcacgctc acgacgattt ccatcatagt cttgaacttc cacactatca gccttgacga
    22561 ttaccaactc ttggtcatgg atttccatgt attggttagt ttcacgaatc atagccatgg
    22621 cgtctgagca gaccatgtta tagccttctc caagaccaat caaaagtggt gatttatttt
    22681 tagctacgta gatgacttca ggatcttgtg agtcaaccaa ggcaaaggca taagaaccac
    22741 ggatgatgtg aagggctttt ttgaaggctt caagaactga gagcccttct tcttccgcaa
    22801 attttccaat caaatgaacg gctatttcag tatctgtctg ccccttgaag tggtgacctg
    22861 caaggtattc ttccttgatt tcaaagtagt tctcaatcac cccattatgc accaagacaa
    22921 aacgttctgt ctcagagcgg tgtgggtgag cattgtcctc agttggtttt ccgtgagtag
    22981 cccaacgagt atgtccgata ccagttgttc cctcaacacc agctgtcttg gcagacaatt
    23041 ctgcaatacg accaacagcc ttcaccaaat ggttatcagc accatctagg acaaaaattc
    23101 ccgcagaatc atagccacgg tattcaagct tttcaagccc ttgaatcaaa atatcagttg
    23161 catttgtgtt tccaacaaca ccaacaattc cacacatagt atatacgaca caggcaagct
    23221 gtgctttctc cttaaaattg gtatagtcta attcatcttt tatagaatca gcaaaaacag
    23281 tatatacttg tttctttcac ttgtcaagag taaaaattgg tatagttcaa attaagctcc
    23341 tgtaagcata aaaactctga ccgattggga taatcagtca gagtcctttt taaaatccat
    23401 tattatcgct taattctttg aaccagtggc ctgatttctt cagacgacgt tcttgcgttt
    23461 ccaagtctaa ttcgaccaaa ccatagcgat ttttatagct gttgagccat gaccagcagt
    23521 caataaaggt ccaaatcaag tagcccttac agttggcacc atcttcaatg gcacggtgaa
    23581 gttcacgaag atgacctttt acaaagtcaa tacggtaatc atcttgaatc attccatctt
    23641 gacggaattt ttcttcccct tcaacaccca taccattctc agtcaacatc cactcaatat
    23701 tgccataatt ttccttgata ttttgggcga tgtcataaat cccttgctca taaatctccc
    23761 aaccacggtg agaattgatt ttacgtccag gcatcacata aggctcgtaa aaatgttctg
    23821 gtaagagtgg actatctgga tgcttagcaa atcgaggagc cataacacgc aaaggttgat
    23881 agtagttcac accaaggaag tccaccgtat tatcacgaat gagttccaac tcttcctctg
    23941 tagcatcagg taaaagaccg tgttcatgca agatttctac caactcctgt ggataagtcc
    24001 ccaagacaga tggatctaag aaagattggg cctgaaaaag ggccgcaata cgagctgcct
    24061 tgacatcagc aggatgctgg ctacgtggat aagccggtgt caagttgagg acaatcccaa
    24121 tcttggaatc aggcaaaagt tcatggcaag ccttaacagc ccggctgctg gccaattgtg
    24181 tatgataggc taccttaaca gctgcctctg catccacctt atgtggataa tgggcatcat
    24241 aaaaataacc aaattctaca ggaacgatgg gctcgttaaa ggtaatccat tgatccacta
    24301 aatctccata agtctcaaaa caaaaacgag catagtcttc ataggctgag actgtcgcct
    24361 tattttccca accatcacca tcctcttgaa gggcaaaagg taaatcaaaa tgatagagat
    24421 tgactaacag acgaattcct ttagccttaa tagcctcaaa gaccttacga taaaaatcca
    24481 caccttgagg gttgactttc ccacagcctt gtggaaaaat ccgtgaccac tgaatagaag
    24541 tccgaaagac tgtgtgacca gtctctaaca aaagctcaat atcccgctcc caattttcat
    24601 aaaaagtcga tgtcttatct gaaccaatcc cattatagta acgatttggc tccacttgga
    24661 accagtaatc ccagagattg tctcccttac cgtcaccagc tacacgtcct tctgtctgcg
    24721 gtccagaagt agaggatccc cagacaaaat cctttggaaa tcttagcata acatttacct
    24781 ctttatctac tcatttctcc cattatacag aaaaaacaag gtaaaaacta gttacatttt
    24841 ttccttgttt ttcttctgat tatagttttt atttcttgct taggatttca agcgtttcaa
    24901 gcacgttatc tgcatgaacc tcaatggtgt caccagttgc cttgatctta acttctacaa
    24961 tgccatcggc cgctttttta ccaacagtga tacggattgg aagaccaatc aagtcactat
    25021 cgctaaattt aacaccgaca cgttcgttac ggtcatctgt caagacttca taaccagctc
    25081 ccatcaagct tgcttcaagt ttttctgtca aggcttgcgc ttcttcatcc ttgacattaa
    25141 cagtaatcaa atgcacatca aatggtgcca attctttagg gaaattgatt ccccaagcgt
    25201 aacggtattc accttttggc gttttgttaa caaagaggcg agcgtgttgc tccatcactg
    25261 ctgaaagaag acggctgaca ccgataccgt aacatcccat gatgattggc acagcacgac
    25321 cattttcatc caagacatct gctcccatgc ttgctgaata gcgagttccg agtttgaaaa
    25381 tatgaccgat ctcaatacca cgcgcaaagt taaggacacc ttgtccatct ggggaaattt
    25441 caccctcacg aacttcacgg atatccacat attctgcagt aaaatcacgg cctgggttca
    25501 caccagtcaa gtggtagcca tcttcgttag caccgacaac tgcattgcga acatcttgca
    25561 ccttacgatc tgcaataatt ttaatattct ctggcaaacc aactggtcca agtgaaccaa
    25621 atcctgcttg aacaacattc gccacttctt cttcgctagc aacgtcaaag aaatctgctc
    25681 ccaagtgatt tttcaacttg acttcgttga gttggtcatt tccaactaga agggctgcaa
    25741 caagctcacc atctgcaatg tagaagaggg ttttaatcgt ttgttcttct ggaacattga
    25801 ggaaagctgc aacttcatca attgatttaa catctggcgt tgcaacacga gtaacttctt
    25861 cttcagcgac aacacggttg cttggtttgt actcgtttgt tgccatttct aagttagctg
    25921 catagctaga ctcacttgag taagcaatgg tatcttcacc agagactatc catttgagca
    25981 attctgcctt gatttcttct tgcacttctg caggaatttc gtcaaatgag gcaactgact
    26041 tgtccaagac aacccagcgg tcaaggtctg tacgagcaga tgtaatggcc ataaattctt
    26101 ggctatcctt accacccatg gctccaccgt caccaataat agccttgaag tctaaaccac
    26161 tacgagtgaa aatacgctca taagctgctt tgtactcatc ataaacacta tccaaactat
    26221 catagttagc gtggaaacta taagcatcct tcatgataaa ctcacgtgta cgaagaagtc
    26281 cattacgcgg gcgtttttca tcacgatact tgggctgaat ttgataaagg ttgagtggca
    26341 attgcttgta agatttaaca gaatcacgga caatagctgt aaaggtttct tcgtgagttg
    26401 gacctaagat aaagtctgat ttttcacggt tttttagttt gtaaaggtct tcaccatagg
    26461 tttcgtaacg acctgattca cgccacaatt ctgcactaag aagggctgga gccaacatct
    26521 caacagcacc aatcttttcg aattcttggc gcatgatgtt tttagctttt tcaatcacac
    26581 ggttggcaag tggtagataa gaataaacac ctgctgaaac ttggcgaaca taaccagcac
    26641 gcaacataag agcatggctg ataacttgag catcgcttgg catttcgcga agcgttggga
    26701 taggcatttt actttgtttc ataatattcc tcgattatct aaaaaagagt cgcataatgt
    26761 cattccaagt cacagcaatc atcaagacaa ccatgatgac cactccggcc aaggtgacat
    26821 aggtttcaat ttcttgtttc aatggtttgc ggcggatggc ttctaggata ttgagcacaa
    26881 tcttaccacc atccaaggct ggaatcggaa taagattaaa aatcccaata ttgatggaaa
    26941 tcattgccaa gaagtacaag atattttcaa ttccattttt agcagcatca ctacttgcct
    27001 taaagatagc aacaggtcca cccaacttgt tcaaatctgg ttggaaaatc agatttttca
    27061 gagctgagag aattcggaga gctgagtcag cagcagttgt aaaaccacct acaaacatgg
    27121 atagaaaatc tgacttaccc cccggttgaa cacctagaag gtaacgacct tgactatctt
    27181 tgggtgtaac agtgacttgt ttgtcactcc ccttttcaga aatagtcaca tccaaagtcg
    27241 gtgccgtctt atctttggtt tctgtttcca cagcttggat caagctttcc cagttgctaa
    27301 cctcatgtga gccaatcttg gtaatttgtg ccgtttctgg tactcctacc ttggccaagg
    27361 caccttgggg catgatatgg aactgattgg tatcaacatc tctgacacca ccctgcataa
    27421 agattaaaac ccaaaaaaca acgacaccta agataaaatt gttcatagga cctgcaaaat
    27481 tggtaatcag tttgccccag atagtcgcat tttgatattg aacatctaaa ggtgcaatcc
    27541 gaacctcagt accatctgct tccacaaccg ttgcatcgtg atccactgca aatgtttttt
    27601 cttcttccag aaccaatcct ttgataaaga gcttgtcttc aaaatcaaac tgggtcacct
    27661 gcatagggag gactgtttga tccaattttt tacctgagag attgatgcgt ttaaccttac
    27721 catcatcagc aagtgtcaaa ctaacaggcg ttcctgtctt gatttcagtt gtatcatcac
    27781 cccaaccggc catgcggaca tagccaccca gaggcaagat tcgaatggta taggccgttc
    27841 catccttgcc aatgtgagca aaaattttag gtcccatacc gatggcaaat tcacgtacta
    27901 aaatccctga tttcttggca aagtagaagt gcccgaactc gtgcaccact acaataatcc
    27961 caaaaactag aataaaggtt aaaattccaa gcatagtgtt tcctccgtct tttgattaaa
    28021 agagtccaaa taagtgcatg attggaaata caagcaacat actatcgaaa cgatccaaaa
    28081 caccaccatg tccagggata aatttcccag aatccttaac accaaaatga cgtttgatcg
    28141 aactttctag taaatcacca aattgtccag caatgctaaa gaaaatagca aagactgaca
    28201 tcttgtaaat tccatatgga agagcaactg tactgtcaac tatcataaag ataatggtta
    28261 ctaaaattgc tcctaaaata ccacccaagg caccctcaag ggttttatta ggcgataccc
    28321 ttggtgctaa ctttcgtttc ccatagttca tcccaacaag ataggcacca ctgtctgtcg
    28381 cccagacgat acacaaggct aagagagcct tgtccaaacc tgcaacacga gcatctagta
    28441 aagcattaaa tccaaagccc acgtagaagc tcatagcaag agggaaaacc gcatcctcaa
    28501 tcgtataaga cttgctaaaa acggtcgttc ctaacatgat tgaaatcaaa acactatagg
    28561 caaccacatt cccatcaact ggcaaaaaag tcaggtaatt ctccaaggga atggtcaatg
    28621 caaaggttgc aaagagggtc aagaggccct ccatcgtcat ggtctctaga cctctcatct
    28681 tcaaaagttc atgcatggct agcatggcta tgattccgat tgctatctga agcaagaggc
    28741 ccccaatcat taaaattggt aggaaaatag ccagggcaat ccctgcaaac aaggttcttt
    28801 tctgtaaatc ctgggtcata tttcctccta aactcctcca aatcggcgat gacgacgatt
    28861 ataggcaaga atagcttcct gcaaggccgc ttcgtcaaaa tcaggccata aggtgtccgt
    28921 aaaataaagc tcactatagg ctccctgcca tggaaggaaa ttgctcaaac gtaattctcc
    28981 actagtacgg ataatcaagt ctgggtctcg taagtcctta ggcaaatgct gagtaaagag
    29041 atagttacca atcaattcct ctgtgatgtc acctgggttg attttggcat ctaaaacatc
    29101 ctgggaaatc aacttaagcg cctgtgtaat ctcagcacgt ccaccatagt taagagcaaa
    29161 attaagaatc aatcctgtgt tgttcttagt caattcctca gccttggtta aagcttcaaa
    29221 ggtttgctta ggcaggcggt ctgtctcccc aatcatttga atcttaacat tattcgcatg
    29281 tagttccggg acataattat cataaaactc tactggcaag ttcatgataa acttgacttc
    29341 ctgatctgga cgggtccagt tttccgtaga aaaagcatag accgtaataa ccttgacgcc
    29401 cagtttgttg gctgccttgg tcacggtttg caatgcttcc atgcccgcct tatgtccaaa
    29461 aactcgcggt tgcatacgtt ttttagccca acggccattg ccatccatga tgatgccgat
    29521 atgagcagga acctgtgtcg gaacctctac ttccacagcc ttatctttct taaaaaatcc
    29581 aaacatgatc ttattcctat tcaaaaatct atcgtttcat tataccatat ttccccattt
    29641 tcttctatca ctaagctatt tattctcagg caccatgccc atttttcaaa aaaataagcc
    29701 gcctgattgg gcgactttat ttttataggg agattattat gaaaaagttt taggagttta
    29761 agttaaggtc ttcttaactt atgaacttag tgtacactcc ctagcttaaa gtttccttaa
    29821 gtatttttaa aaatcaaatt tttccatttc tcctgccaat ttttcttgga taaacgtgtt
    29881 tgatagagtt ccattcggtc ttcattttct aagaaatgag gagttggacg aacttgaaaa
    29941 ttcaaaatat cctccaaacc ataaggtgca tagagttcaa aatctgattc ttcattcaag
    30001 cgcagtccaa ctgccgtaca ccgttctgga tacttactca tagcatcacg agaactggta
    30061 taggaagcag tgtgaggact gtgctggtgc atatagacct gatttttcaa ttcccactgg
    30121 tactgaggaa aatcctctct cagctttttc tccagtaata aggtttcctc ataagaaaaa
    30181 tctggatcaa agaaaatcac atctatatct gtttcatgat caaaagggga tttgtctgac
    30241 aaaagattcc agatgaaatt tctgacagaa cctgctgcca actacgagtc tttcaaacca
    30301 aggtctcgga tgatcgtcag aatggccatc atatctggat tttctctaaa agcctctaag
    30361 atttcttgct tatttttcac tgtattcata acctaagtgc tcatatgcct tagcagtcgc
    30421 cacccgtcca gaccgtgtcc gcatgataaa acctttttga atcaagtaag gctcatacat
    30481 gtcttcaact gtctcacgct cttcggcgat attgacagaa agagttccta gaccaacagg
    30541 tcctccactg tacatctcaa tcatggtacg aaggattttt tgatccacat agtccaaacc
    30601 ttcatggtca acatccagca tagtcaaagc cttatcggta ataatatcat cgattacccc
    30661 attccccatt atctgggcaa aatcgcgcac gcgcttgagg agacgattgg caatacgagg
    30721 ggttccacga ctacgtaggg ccaactcaga tgctgcctca tgagtgattt ccatctcaaa
    30781 aatatctgcc gtccgctcga caatttctgt caagtcagca tgggcataat actccatatg
    30841 acctgtaatc ccaaaacgtg cccgtagtgg atttgagagc ataccagccc gagtcgtcgc
    30901 accaatcaag gtaaaaggag gcaactccaa atgaacactg cgactgcctt caccagcccc
    30961 aatcataata tcgatgtaga agtcctccat ggcactataa agcacttctt ccactgacat
    31021 gggtaagcga tgaatctcgt caataaagag gacatctcca ggctctaact cattcaaaat
    31081 cgctaccaaa tcacccgctt tttcgataac aggaccagac gtttgcttga gattgactcc
    31141 cagttcattg gcaatgacaa aagccatggt tgttttccca agccctggag ggccaaataa
    31201 gagcacatga tccagcgctt catcccgcat tttagcggct tcgataaaga tctgaagctg
    31261 atccttaacc ttatcctgac caatatattc acgtaaatac tgaggacgga gcgtgcgttc
    31321 tactaactcc tcatcaccca tcatctcatt atctaaaatt ctactcatgg ctctattata
    31381 tcaaaaaaac aagccacaaa caaaaaagcc acctgattgg gtgactccta agtttagcac
    31441 ttatgtggta taatattata cggcacttct acaccgccta cgaaaggagg tgagatagcc
    31501 catgatggaa ttagtactca aaactatcat cggaccaatt gtggtcggtg tcgttcttcg
    31561 tatagtcgat aaatggctaa acaaggacaa atagtgtcaa aaaagacctc aagcttattt
    31621 ggtcgtgagc ttggggtctt ttctagccta tgatatagaa ctagtactca attccttttt
    31681 attatcccat agttcacgaa ttttgtcaaa ggtttacatt ttcttcaacc gctgtacgac
    31741 aagacggtta agattaagag aacgttaggg attctatcaa tttcatagaa attttgattt
    31801 cgtaaacgaa gagacaatct tacatgtcac ttctcattta atacgccact actagacaag
    31861 caaaatcatt attacagtag ttccagttct tcaattaaca gtcacttaca atcaaattga
    31921 gtttgaacta gctgaagcga ccacagacct atttcttagt tatattcgct aaaaaagtcc
    31981 ccgccaattc cccgaccaaa atccgaaaaa taccgaaaaa tatcgaaaaa ttatttttag
    32041 aatagtcccc aaaagcctga aatagagcta aaaaactcca cctgattggg tggagttaag
    32101 ggagattatt atgaaaaaga aaagtttagg attttattaa ataaagttag gaggtcttta
    32161 tttaataact acatgataca agacgaaact taaaactagc ttaacttttc taaaatttta
    32221 ctattttgca aaaaatttct atcaccagca cctcaccacg caaaaaagcc acctgattgg
    32281 gtggcttcat taggagattg tgatgaaaaa agtttaggat ttt
//
LOCUS       DAVLRT010000016        31543 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLRT010000016 DAVLRT010000000
VERSION     DAVLRT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.016"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            355..1518
                     /locus_tag="VXM85_000732"
     CDS             355..1518
                     /locus_tag="VXM85_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1345355.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1515..2192
                     /locus_tag="VXM85_000733"
     CDS             1515..2192
                     /locus_tag="VXM85_000733"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1345356.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2182..3036
                     /locus_tag="VXM85_000734"
     CDS             2182..3036
                     /locus_tag="VXM85_000734"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1345357.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3055..4122
                     /locus_tag="VXM85_000735"
     CDS             3055..4122
                     /locus_tag="VXM85_000735"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1345358.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4132..5298
                     /gene="aroC"
                     /locus_tag="VXM85_000736"
     CDS             4132..5298
                     /gene="aroC"
                     /locus_tag="VXM85_000736"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1345359.1"
                     /translation="MRYLTAGESHGPRLTAIIDGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5308..6411
                     /locus_tag="VXM85_000737"
     CDS             5308..6411
                     /locus_tag="VXM85_000737"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1345360.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6422..6760
                     /locus_tag="VXM85_000738"
     CDS             6422..6760
                     /locus_tag="VXM85_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1345361.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6852..8135
                     /gene="aroA"
                     /locus_tag="VXM85_000739"
     CDS             6852..8135
                     /gene="aroA"
                     /locus_tag="VXM85_000739"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1345362.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLSPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8128..8604
                     /locus_tag="VXM85_000740"
     CDS             8128..8604
                     /locus_tag="VXM85_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1345363.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGESAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8601..9449
                     /gene="pheA"
                     /locus_tag="VXM85_000741"
     CDS             8601..9449
                     /gene="pheA"
                     /locus_tag="VXM85_000741"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1345364.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9446..10720
                     /gene="psr"
                     /locus_tag="VXM85_000742"
     CDS             9446..10720
                     /gene="psr"
                     /locus_tag="VXM85_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1345365.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     RNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSSPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10999..11844
                     /locus_tag="VXM85_000743"
     CDS             10999..11844
                     /locus_tag="VXM85_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1345366.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11834..12937
                     /locus_tag="VXM85_000744"
     CDS             11834..12937
                     /locus_tag="VXM85_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1345367.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12939..13925
                     /locus_tag="VXM85_000745"
     CDS             12939..13925
                     /locus_tag="VXM85_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1345368.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13941..15374
                     /locus_tag="VXM85_000746"
     CDS             13941..15374
                     /locus_tag="VXM85_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1345369.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            15403..16437
                     /locus_tag="VXM85_000747"
     CDS             15403..16437
                     /locus_tag="VXM85_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1345370.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16579..17316
                     /gene="mecA"
                     /locus_tag="VXM85_000748"
     CDS             16579..17316
                     /gene="mecA"
                     /locus_tag="VXM85_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1345371.1"
                     /translation="MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFAEDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17467..18753
                     /locus_tag="VXM85_000749"
     CDS             17467..18753
                     /locus_tag="VXM85_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1345372.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVVHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18755..19624
                     /locus_tag="VXM85_000750"
     CDS             18755..19624
                     /locus_tag="VXM85_000750"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1345373.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19874..20812
                     /gene="msrB"
                     /locus_tag="VXM85_000751"
     CDS             19874..20812
                     /gene="msrB"
                     /locus_tag="VXM85_000751"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1345374.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20870..22594
                     /locus_tag="VXM85_000752"
     CDS             20870..22594
                     /locus_tag="VXM85_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345375.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISTELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISVKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            22596..24347
                     /locus_tag="VXM85_000753"
     CDS             22596..24347
                     /locus_tag="VXM85_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345376.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     TSDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRF
                     YPISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVI
                     AAAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATS
                     SIDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMD
                     RKGKYYQMQKAAAFSSE"
     gene            24694..25953
                     /locus_tag="VXM85_000754"
     CDS             24694..25953
                     /locus_tag="VXM85_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1345377.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26029..26164
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26203..26703
                     /locus_tag="VXM85_000755"
     CDS             26203..26703
                     /locus_tag="VXM85_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1345378.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26779..27147
                     /gene="rplL"
                     /locus_tag="VXM85_000756"
     CDS             26779..27147
                     /gene="rplL"
                     /locus_tag="VXM85_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1345379.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27334..27504
                     /locus_tag="VXM85_000757"
     CDS             27334..27504
                     /locus_tag="VXM85_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345380.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27504..27752
                     /locus_tag="VXM85_000758"
                     /pseudo
     CDS             27504..27752
                     /locus_tag="VXM85_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27766..28106
                     /locus_tag="VXM85_000759"
                     /pseudo
     CDS             27766..28106
                     /locus_tag="VXM85_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28099..28479
                     /locus_tag="VXM85_000760"
     CDS             28099..28479
                     /locus_tag="VXM85_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1345381.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            28492..28635
                     /locus_tag="VXM85_000761"
     CDS             28492..28635
                     /locus_tag="VXM85_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345382.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            28728..29315
                     /locus_tag="VXM85_000762"
     CDS             28728..29315
                     /locus_tag="VXM85_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1345383.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWSLLVYSLLLF"
     gene            29517..29654
                     /locus_tag="VXM85_000763"
     CDS             29517..29654
                     /locus_tag="VXM85_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV1345384.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            29944..31347
                     /locus_tag="VXM85_000764"
     CDS             29944..31347
                     /locus_tag="VXM85_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV1345385.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVVIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYWYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
BASE COUNT         9501 a         5678 c         7020 g         9344 t
ORIGIN      
        1 caaaaggctt ttctataaaa tcaaattttc cagataccta ttgaagcaga tttatttctt
       61 catctttctt ctactacctt tgtcactaaa tttgacagac acatattgct gattcaacct
      121 ctctacctat aatctcacag ttaacctgag gctagtttca tagtttgcta tttgattttc
      181 gttgataaaa agaaatgctc ctccaagatg gaagagcatt ttttattgtg attcttacac
      241 atttacttcc tagttttgca ggaagtctag tataccattt tttaaaagaa gagtaaaaaa
      301 gcaagggtag tttctttttt gagaattgtc taaaagtttg atataatggt agttatgaat
      361 agaataagag tcagcaaaag ggttgaaaag aagcttgcta aggggctagt tttactagaa
      421 gccagtgatc ttgagaatgt caatcttaag gatcaggaag tagaggtgca gggtcaggaa
      481 ggaaactttc ttgggactgc ctacctttct cagcaaaaca agggcttggg ctggtttgtc
      541 agcaaagaca aggtggcctt caatcaagct ttctttgaaa cgttgtttag aaaagccaaa
      601 gaaaagagaa acgcctacta tcaagatgat ttgacaactg cctttcgtct ctttaatcaa
      661 gagggagatg gctttggggg tctgacagtg gacctttatg gcgactacgc cgtcttttct
      721 tggtataact cttatgttta tcagattcgt cagactatat cagaagcctt tagacaggtt
      781 ttccctgagg ttttaggagc ttatgagaaa atccgcttta agggtttgga ctatgaatct
      841 gcccatgttt atggtcaaga agcacctgac tttttcactg ttttagaaaa tggtgtcctg
      901 tatcaagtct ttatgaatga tggcttgatg acaggaattt tcctagacca gcatgaggtt
      961 cgcggtagtt tagttgacgg cttggctatg ggtaaatcct tactcaatat gttttcctac
     1021 acagcggctt tttcagtagc tgcggccatg ggaggagcta gccagacaac ttctgttgat
     1081 ctagccaaac gttcacgaga attgtctcaa gcgcattttc aggcaaatgg gctcagcaca
     1141 gacgagcatc gttttatagt catggatgtc tttgagtatt tcaaatatgc caaacgcaaa
     1201 gacttgacct acgatgtgat tgtcctagat ccgcctagct ttgctcggaa taaaaaacaa
     1261 actttctctg tggccaagga ttatcacaag ttgatttccc agagtcttga gattttaaat
     1321 ccgggaggga ttatcattgc cagtaccaat gctgccaatg tttcccgtca gaaatttaca
     1381 gaacaaattg ataaaggttt tgcaggaaga agttaccaga ttttaaacaa atatggtctt
     1441 ccagcagatt ttgcctataa taaaaaagat gaaagtagta attacctcaa ggtgattagt
     1501 atgaaggtta gtaaatgaaa ttaatcgttt cagtaatgcc aagaagttta gaggaggctc
     1561 aggctctgga tgccacgagg tacttggatg ccgacatcat tgaatggcgt gccgactatc
     1621 tgcctaaaga agcgattttg caggtagctc cagccatttt tgaaaaattc gcaggccgtg
     1681 agttggtttt cacgctacga actcgctccg aaggtggaga aatcgacctt tctccagaag
     1741 agtatatcca tctaatcaag gaagttgcgc aactctatca accagactat attgattttg
     1801 agtactatag ctacaaggat gtttttgagg aaatgctgga cttcccaaat cttgttttga
     1861 gttaccataa tttccaagaa acacctgaga acatgatgga aatcttgtca gagttgacga
     1921 tcctaaatcc aaaacttgtt aaggttgcgg tgatggctca cacggagcag gatgtcttag
     1981 acttgatgaa ctatacacga ggctttaaaa ccctcaatcc tgaacaggaa tatgtgacca
     2041 tttctatggg taaggtgggc aaggtctctc gtatcactgc ggatgtgact ggttcgagtt
     2101 ggtcttttgc cagtctggat gaggttagtg cccctgggca gatttctcta gctagcatga
     2161 aaaaaatcag ggagattttg gatgaagctt gatggctata cacgtttagc tgcagttgtt
     2221 gccaatccta ttaagcattc tatttctccc ttcatccaca atagagcctt tgaggcgaca
     2281 gctaccaacg gtgcttatgt ggcttgggag attgaagcga gtgacttggc agaaacagtg
     2341 gccaatattc gtcgctacca gatgtttggc atcaatctgt ccatgcccta taaggagcag
     2401 gtgattcctt atttggatga gctaagcgat gaagcgcgct tgattggtgc ggttaatacg
     2461 gttgtcaatg agaatggcaa tttaattgga tataatacag atggcaaggg attttttaag
     2521 tgcttgcctt cttttacaat ttcaggtaaa aagatgaccc tgctgggtgc aggtggtgcg
     2581 gctaaatcaa tcttggcaca ggctattttg gatggcgtca gtcagatttc ggtctttgtt
     2641 cgttccgttt ctatggaaaa aacaagacct tacctagaca agttacagga gcagacaggc
     2701 tttaaagtgg atttgtgtgc tttagaatat gtttctgaac tgcaagcaag gattgccgag
     2761 tcggatttgc tagttaatgc caccagtgtg ggcatggatg gccaatcctc tcctgttcct
     2821 gaaaacatag tcttaccaga aactctttta gttgcagata tcatatacca accctttgaa
     2881 acaccgtttt tgaaatgggc tagaagtcag ggcaatccag cagtcaatgg tctgggaatg
     2941 ttgctctatc aagctgcaga agcttttcaa ctgtggacag gcaaggaaat gccgacagaa
     3001 gagatttggc agtctttaac agaaaaatac caataaagaa agggagactc tcctatgaaa
     3061 atcagaatcg atattcctca tcatccttat gatattcaga ttgaaaaagg ttgtatggcc
     3121 caggctggtc agtggttgcg agaactctgg caaccgcaaa aggtagtcat tgtgacagat
     3181 aaccatgtag cctctctcta tgcagagaag gtcaagctca gcctagaaga tgctggtttt
     3241 caggtagctg tttttgattt cttagaaggt gaagaaagaa agaatttaac tactgttcag
     3301 aaagtctatg aatttctagt caagcaaggt ctgactcgta gcgatggaat cgttgctctt
     3361 ggtggtggcg ttgttgggga cctggctggc tttgtagcct ctacctatat gcggggtatt
     3421 cactttgttc agattccgac tagtttgaca gctcaggttg attcttctat cggtggaaag
     3481 acaggcgtta acacaccatt tgctaaaaat atggtgggga cctttgccca accagatggg
     3541 gttctgattg atccacttgt tcttgaaacc ctcggaaaaa gagagttgat tgaagggatg
     3601 ggtgaagtta tcaagtatgg cttgattgag gatccagaac tgtgggctct cttgacggga
     3661 ctgaatggtt ctgttgagag tattttggaa catgcagaga ccttgattga acattcttgt
     3721 caggtgaagc gcaagatggt ggttgaagat gagttggaca atggtattcg tctttacctc
     3781 aattttggcc acactattgg ccatgccatc gaagcgactg ccggttatgg caaggtcatg
     3841 catggagagg ctgttgccat gggaatggta cagatttcca agattgctga ggaaaaaggc
     3901 ctcatgccag ctggcattac ccaatctatc acagagatgt gtcagaaatt cggcttgcct
     3961 gttgactatg aaaattggga agttgacaag ctttatcagg ctcttactca tgacaagaaa
     4021 gcgcgtggca acaccttgaa attggtcttg gtgccagagc ttggttcagc gatcattcac
     4081 ccagtttctc tggaagagat gaaagactac ttggtaaaat aaggagagtg tatgagatat
     4141 ttaactgcag gagaatcaca cggcccccgt ctaacagcta ttattgatgg aattccagct
     4201 ggacttccat tgacagctga ggatatcaat gaggacctta gacgccgtca gggtggctac
     4261 ggtcgtggtg gtcgtatgaa gattgagaat gaccaggttg tctttacttc gggcgttcgc
     4321 cacgggaaga cgacaggggc gcctattact atggatgtca tcaataagga ccaccagaaa
     4381 tggctggaca tcatgtctgc ggaggacatt gaagaccgtc ttaaaagcaa gcggaaaatt
     4441 actcatcctc gcccaggtca tgccgatttg gttgggggga ttaagtaccg ttttgatgat
     4501 ttgcgaaatt ctttggagcg ttcatcagct cgtgaaacca ccatgcgggt ggcagttggt
     4561 gcagtagcca aacgcctctt ggctgagttg gatatggaga ttgccaacca tgtcgtggtc
     4621 tttggtggca aggaaatcga tgttcctgaa aatctgacag tcgctgaaat taagcaaaga
     4681 gctgcccagt ctgaagtttc tattgtcaac caagaacgag aacaggaaat caaggactat
     4741 attgaccaaa tcaaacgtga tggtgatacc atcggtgggg ttgtggagac agtcgtcgga
     4801 ggcgttccag ttggtcttgg ttcctatgtc caatgggata gaaaattgga tgcaagattg
     4861 gctcaagctg ttgtctctat caatgccttt aaaggggtgg aatttggtct tggctttgag
     4921 gctggttatc gtaaaggcag ccaagttatg gatgaaattc tctggtctaa agaagacggt
     4981 tatactcgcc gtaccaataa tctaggtggt tttgaaggtg gtatgactaa tgggcaaccc
     5041 atcgttgttc gtggggtcat gaaacccatt cctactcttt ataaacctct tatgagtgtg
     5101 gatatcgaaa cccacgaacc ttacaaggca accgtggaga gaagtgatcc gactgctctt
     5161 ccagctgcag gaatggtcat ggaagcagtt gtagcaacgg ttctggcgca agaaatcctc
     5221 gaaaaattct catcagataa tcttgaggaa ctaaaagaag cggtagccaa acaccgagac
     5281 tatacaaaga actattaagg agttcctatg gcaaaaacaa tctatatcgc aggtcttggg
     5341 ttgattggag cctctatggc acttggtatc aaacgcgatc atccagatta tgaaatttta
     5401 ggttataatc gtagtcaagc ttcgagagat atcgccttga aagaaggcat gattgaccgt
     5461 gcaacggatg attttgctag ttttgctcct ttggcagatg tcattatcct cagcttgcca
     5521 atcaaacaaa ctattgcttt cattaaggag ttggccaatt tggatttgcg agaaggcgtt
     5581 attatttcag atgctggttc gaccaagtca accattgtgg atgcggcgga gcagtatttg
     5641 gctggcaagt ctgttcgctt tgtcggggcc catcccatgg ctggtagtca caagacaggg
     5701 gctgcttcgg cagatgtcaa tctttttgaa aatgcctatt atatctttac accttcaagc
     5761 ctgacaagtc aggacacgct taaggaaatg aaggatctgc tttcaggtct tcatgctcgt
     5821 tttatcgaga ttgatgccaa ggagcatgat cgtgtcactt ctcagattag ccattttcct
     5881 catattttgg cttctagtct catggagcag actgcggtct atgctcaaga gcatgagatg
     5941 gcaaggcgct ttgcggcagg tggttttcga gatatgaccc gaattgcgga aagcgagcca
     6001 ggaatgtgga cctccattct cttgtccaat agcgagatca ttctggatag aattgaggat
     6061 ttcaaggaac gtttggaagc gattggtcag gccattagta agggagatga agagcaaatt
     6121 tggaactttt ttaaccaagc gcgtgaacaa cgccagacca tggaaatcca taagcgtggt
     6181 ggtgtggata gctcttacga cctctatgtt gacgttcctg atgaagaaga tgtcatcctg
     6241 cggattttgg aactgctacg tggaacttcc ttggttaata tccacatcaa cgaggaaaat
     6301 cgtgaggata ttcacggaat cctacaaatt tcatttaaaa atgctcaaga cttggaaaga
     6361 gctgagcatc tcataacaga aaatactgac tatacagtcg tcatcaaata aggagaagaa
     6421 catgtcaaat atttacgata gtgcaaacga actcagtcgc ggtctacgcg gattaccaga
     6481 atacaaggct gttaaagcag ctaaagatgc gattgcagca gatgctgagg caagcaaaat
     6541 ctttacagat tatcttgcct ttcaagagga aattcaaaaa ctagcacaga caggtcaaat
     6601 gccagatgcg tccttccaag ctaagatgga aggctttggt aaacagattc aaggaaatag
     6661 cctcttgtca gaattcttta ccaagcaaca acaattggcc atttaccttt ctgacattga
     6721 aaaaattgtt tttgaaccag tttcagaatt gctgaaataa gaaaagaact atcctaaagt
     6781 caggaatttt taaggaattt ctggcttttt atgataaaat aggtaaaagt attgcaagta
     6841 tgaggtccag tatgaaacta aaaacaaaca ttcgccattt acatggtagt atccgcgtcc
     6901 caggtgacaa gtctatcagc caccgttcca ttatctttgg aagtttggct gagggtgaga
     6961 ccaaggttta tgatattctg cgaggcgaag acgttctttc gaccatgcag gtttttcgtg
     7021 accttggtgt tgaaattgag gataaagatg gggttattac cattcaaggt gtaggcatgg
     7081 ctggcttaaa agcgccgcaa aatgccctta atatgggaaa ttctggcacc tcgattcgcc
     7141 tgatttcagg tgtccttgct ggtgcagatt tcgaagtaga gatgtttgga gatgatagtc
     7201 tttccaaacg tcctatggac cgtgtgaccc ttccactgaa aaaaatgggc gtcagcattt
     7261 cagggcaaac tgaacgagac ttgcctcccc ttcgcttaaa agggacgaaa aacctaagcc
     7321 ctattcatta tgagttgcca attgcctctg cccaagtcaa gtcagccttg atgtttgcag
     7381 ccttacaagc taagggggag tcagttatta tcgaaaaaga atacacccgt aatcatactg
     7441 aagatatgtt gcaacaattt ggtggtcatt taagtgtgga tggtaagaaa atcacagtcc
     7501 aagggccaca aaaattgaca ggacagaagg tggtcgtacc aggagatatt tccagtgcag
     7561 ccttttggtt agtcgcaggt ttgattgctc caaattctcg tctagtgctg cagaatgtgg
     7621 ggatcaacga aactcgcacc ggtattattg atgtcattcg tgccatgggt ggaaaattgg
     7681 aaataactga aatcgatcca gtcgctaaat ctgcaacctt gattgttgag tcttctgact
     7741 tgaaaggaac agagatttgt ggcgctttga ttccacgttt gattgatgaa ttgcctatta
     7801 ttgccctact tgcgacccaa gcccaaggtg taacagttat taaagatgct gaggagctca
     7861 aggtcaagga aacagaccgt attcaggttg tggcagacgc cttaaatagt atgggagcag
     7921 atattactcc tacggcagat gggatgatta tcaaaggaaa atcagctctt cacggtgcta
     7981 gagtcaatac gtttggtgac caccgtatcg gcatgatgac agctatcgca gccctattgg
     8041 ttgcagatgg agaggtggag cttgaccgtg cagaagccat caataccagc tatcctagtt
     8101 tctttgatga tttggagagc ttgattcatg gctaaggtgt tattagggtt tatgggggct
     8161 ggaaaatcaa ctattgcaag aggcttggac cctaattacc ttgatatgga tgctctgatt
     8221 gagaagcgcc taggtatgtc cattgcgaat tttttcgctg aaaagggaga atcagccttt
     8281 cgtcaggtag aatcagaagt cctagctgat ttactacaaa cagaccaagt cgtgtcaact
     8341 ggaggaggag tggttatttc tcagagaaat cgtgacttac tcaagactaa tacagataac
     8401 atctacctga aagcagattt tgaaaccctc tacctacgta tcgtagctga taaggacaat
     8461 cagcgaccgc tttttctaaa taatagcaag gaagaactag tagctatttt tcaagaaaga
     8521 caggcttggt atgaggaagt ggctagtcgg gttttggatg tgaccaagct aagcccagag
     8581 gaaattatag aggaactaag atgaaaattg cttatctagg tcccaaagga tcattttcac
     8641 accacgttgt gcagacagct tttcctcatg aggaattgca ggcttttgcc aacattacag
     8701 atgttatcaa ggcttatgag caaggattag tggactattc tgtggtgcca gttgaaaatt
     8761 ctattgaggg tagtgttcat gaaaccttgg actatctttt tcatcaggct cacatccaag
     8821 cagtagcaga aatcgttcag cctattcatc agcagttgat ggtggttcca ggccatacta
     8881 agattgaaaa gattttttca catccacagg ccttggctca aggaaagaaa ttcatcgatg
     8941 aacaatatcc agaggctcaa atcgaggtaa cagctagtac agcttatgcg gcccgtttta
     9001 tttccgaaca tccagaccag ccctttgcag cagttgcacc tagaagttct gctgaagaat
     9061 atggattgga actgattgcc gaggatattc aggaaatgga agccaatttc acacgtttct
     9121 gggttctagg agctgaaaag cctagtattc ccttgcaagc acaaactgaa aagatgagtt
     9181 tggccttgac attacctgac aaccttccag gtgcacttta taaggccctg tcgacctttg
     9241 cttggcgagg aattgacttg acaaaaattg aaagtcgtcc actcaagaca gcactgggtg
     9301 aatacttttt cattatcgat gtggattata ccgataagga cttggtccac tttgcccaaa
     9361 aagaattaga agcgattgga atccagtata aaattctggg tgcctatcct atttatccaa
     9421 tatcagacca tggaaaggag agaagatgag taaagaaaat cccttaagtc atcatgagca
     9481 gttgcgttat gattatttgc taaaaaatat tcactacctc aatgagagag aaaaaaatga
     9541 gtttgcctat ttgcaagaaa agctaactct tgctaggaga aatagtagct ctagcttgga
     9601 acaagaaaga gaagagcagg ttgacttacc aagttatgcg aaccggagtc gctcacaatc
     9661 caaatcacaa gcactctctt cccctccaaa aaagaaaaga cggaagctcc gtcttaaacg
     9721 aatttttatg gtgatttttt cacttttggt ctgtgtggct ttggccatgg tattcatgtt
     9781 tttgcgtggt taccaagatg ctagcgcaaa gaaaactgct gatgcccggg cagctcaagt
     9841 agaagtcttt aatggtcagg acactagaga tggagttaat attttaatca tgggtactga
     9901 tggtcgaatc ggccagaaca gtgttgagac acgaactgac tctattatgg tattaaatgt
     9961 cgggggctca gataagaaaa tgaagctagt cagtttcatg cgtgataatt tggtctatat
    10021 agatggttat agtcaagtga ttaacggtag aaaacagaca gataacaagt taaacgtagc
    10081 ctacgagtta ggagaacaag aggggcaaaa aggggcagaa atggttcgcc aagtcttgaa
    10141 agataatttt gacttggaca ttaagtacta cgccttggtt gatttccagg cctttgccac
    10201 agcgattgac acacttttcc ctgatggggt gacgattgat gctcaatttt caacattgaa
    10261 tgggcgtccc ctaacagaag ctacagtcgg agatgattta tacgctaccg agactgagtc
    10321 tccaacccaa accattaaag tcggaaaaca gcagatgaat ggctcgaccc tgctcaatta
    10381 tgctcgtttc cgtgatgatg atgaggcgga ttacggccgt acaaaaagac agcaacaagt
    10441 tttaacagca attttagagc aaattaaaga tcccactaaa cttttcactg gttctgaagc
    10501 tcttggaaag gtttttgcta tgacctcaac caacgtaccc tatactttcc tcctaacaaa
    10561 cggtttatct gttttggatg gagcaaaaaa tggtattgaa aaattgacga ttccagaact
    10621 gggtgactgg gtagatgcct atgatgttta tggaggcttg ggcttgctgg ttgatcaaaa
    10681 caaatatcaa accaagctcg ctcaaatggg cttgagataa gataatactc aatgaaaatc
    10741 aaagtgcaaa ctaggaagct agccgcaggc tgctaaaaac acagttttga ggttatagat
    10801 aagactgacg aagtcagtta cctataccta cggcaaggtg aagctgacgt gctttgaaga
    10861 gattttcgaa gagtataaga cagaaaaatc aaagtttgat agctatttgg atagcagtca
    10921 ggctttgatt ttttacagtc tgcaatagat tttcacccct tgtttgtggt ataatgaaac
    10981 gatacgatag aaagaataat gaacaatatg actgatttaa aagcaattca ggctcgtagt
    11041 ctggagatgg ctgaatattt tgtggccttt tgtaaagaac atgatttgct ctgttatctc
    11101 tgtggcggag gcgctattgg tacccttcga aacaagggat ttattccttg ggacgacgac
    11161 ctagactttt ttatgcctcg taaagattat gagaaattag cagaattatg gcctcgttat
    11221 gcagatgaac gttatttctt gtcaaagagt tacaaggatt ttgttgatcg taatcttttt
    11281 attaccattc gtgacaagga aaccacctgt atcaagcctt atcagcagga tttggatttg
    11341 ccacatggtc tggccttgga tgttttgcct ttggattatt atccgaaaga tccagctgag
    11401 cggaaaaaac aggttcgttg ggccttgatt tattcactct tttgtgcgca aactattcca
    11461 gaaaagcatg gtgctctcat gaaatgggga agtcgcattt tactgggttt gactccaaaa
    11521 tctctccgtt atcgcatctg gaaaaaagct gagaaagaaa tgactaagta tgatttggct
    11581 gattgtgatg gcattacaga attatgctca ggtcctggct acatgagaaa caagtaccca
    11641 atcacatctt ttgaagacaa tcttttcttg ccatttgagg gaacagagat gcctattcca
    11701 atcggctatg atgtctatct cagaactgct tttggggatt atatgacgcc tccaccagca
    11761 gacaagcagg taccgcatca tgatgctgtc atcgctgata tggataagtc ttatacagaa
    11821 tacaagggag aatatggtgg ctaagaaaaa aatcttattt tttatgtggt ctttttctct
    11881 tggaggtggt gcagagaaga ttctatcaac cattgtttca aatctggatc cagaaaagta
    11941 tgatattgat attcttgaaa tggagcactt tgacaaggga tatgaatctg ttccaaagca
    12001 tgtacgcatt ttaaaatccc ttcaagatta tcgccaaacc agatggttac gagctttttt
    12061 gtggagaatg agaatttatt ttccaagact ggctcgtcgt ttgcttgtaa aagatgatta
    12121 tgatgttgaa gtttctttta ccattatgaa tccaccactg ttgttctcta aaagaagaga
    12181 agtcaagaag atatcttgga ttcatggaag tattgaagaa cttcttaagg atagctctaa
    12241 aagagaatca catagaagcc agttggatgc tgcgaataca attgtaggga tttcaaaaaa
    12301 gaccagcaat tctatcaagg aagtttatcc agattatgct tctaaattac agacaatcta
    12361 caatggatat gattttcaga ctattctaga aaaatctcaa gagaagatcg atatcgagat
    12421 tgctcctcaa agtatctgta ctatcggacg gattgaggaa aataagggtt ctgaccgtgt
    12481 agtggaagtg atacgattat tacaccaaga gggaaaaaac tatcatctct attttatcgg
    12541 ggctggtgat atggaagagg aactgaaaaa acgagtcaaa gagtatggga ttgaggacta
    12601 tgtacatttc cttggttatc aaaaaaatcc ttatcagtat ttatctcaga cgaaagttct
    12661 cttgtctatg tctaaacaag aaggttttcc tggagtgtat gtggaggcct tgagtctggg
    12721 actccctttt atctctacgg acgttggagg ggctgaggaa ttatcccaag aaggacgatt
    12781 tggacaaatc attgagagca atcaagaggc agctcaggcg attactaatt acatgacttc
    12841 tgcctcaaac tttgatgtcg atgaggctag ccaattcatt caacaattta caattacaaa
    12901 acaaatcgaa caagtagaaa aactattaga ggagtagcat ggaaactgca ttaattagtg
    12961 tgattgtgcc agtctataat gtggcgcagt acctagaaaa atcgatagct tccattcaga
    13021 agcagatcta tcaaaatctg gaaattattc ttgttgatga tggtgcaaca gatgaaagtg
    13081 gtcgcttgtg tgactcaatc gctgaacaag atgacagggt gtcagtgctt cataaaaaga
    13141 acgaaggatt gtcgcaagca cgaaatgatg ggatgaagca ggctcacggg gattatctga
    13201 tttttattga ctcagatgat tatatccatc cagaaatgat tcagagctta tatgagcaat
    13261 tagttcaaga agatgcggat gtttcaagct gtggtgtcat gaatgtctat gctaatgatg
    13321 aaagcccaca gtcagccaat caggatgact attttgtctg tgattctcaa acatttctaa
    13381 aggaatacct cataggtgaa aaaatacctg ggacgatttg caataagcta atcaagagac
    13441 agattgcaac tgccctatcc tttcctaagg ggttgattta cgaagatgcc tattaccatt
    13501 ttgatttaat caagttggcc aagaagtatg tggttaatac taaaccctat tattactatt
    13561 tccatagagg ggatagtatt acgaccaaac cctatgcaga gaaggattta gcctatattg
    13621 atatctacca aaagttttat aatgaagttg tgaaaaacta tcctgacttg aaagaggtcg
    13681 cttttttcag attggcctat gcccacttct ttattctgga taagatgttg ctagatgatc
    13741 agtataaaca gtttgaagcc tattctcaga ttcatcgttt tttaaaaggc catgcctttg
    13801 ctatttctag gaatccaatt ttccgtaagg ggagaagaat tagtgctttg gccctattca
    13861 taaatatttc cttatatcga ttcttattac tgaaaaatat tgaaaaatct aaaaaattac
    13921 attagaacgg gggtgaccaa gtgattgatg ggaaacgatt attatttagt ttgaccatag
    13981 tcagttatgc cttgacgcta gtaagtggaa ttgtgtatct gtttaataat aataatgtta
    14041 gcttactttc tactttattg ttcttgttgg ttagtagctt aattgcttgt tggaatgata
    14101 tcaagtatta cttaatccat tttattttct atttaaccat ttttgtattt ctggtatcaa
    14161 gaccgaccat tgattatttt agggatggtg ctttggatac ctatcatcca atagcctatc
    14221 gttttgcctt tatagttgtc atggtttcga ttctgggctt gaccacagga ggcattctgg
    14281 ctcgttactt catagctagg aagaaaataa aagtagcaaa tataggaaat tctctaaaag
    14341 aggtttatat caagcggtta cgctttgtat cactaggagt ttttcttcta acctatcctt
    14401 tctatttcat tcggttattt gaacggctct tgtatcgttt gcagacttcc tactatgcct
    14461 actatgcaaa ttttgaaagt aaactgcctt attttaccta cattttgtct acctttacgg
    14521 tctatgcaat gtgtatgtat ctggcaacca agccaaagaa attgcaggcc acagcagtgc
    14581 ttgtctcctt tattgcagct aatactattc atttggcaat tgggacacga aatcccttta
    14641 ttttaagtat tttatttgct tttgtttatt actttatgcg ggagcaaact gaaaaaggaa
    14701 aatggattgg gtttaaagaa aagttagcga tttttgtagg ttctcctatt ctcatgttag
    14761 cgatgggagt actcaattat gtacgggata atgtccaagt ttcccataca ggtttctggg
    14821 atatcttact tgactttatc tataaacaag ggactagttt tggtgttctg gctcgaggtt
    14881 ttctatttaa cagtagcctc ccttaccgag atttccgtaa ttttactttt ggtcctgttc
    14941 ttgattattt tgcaaggggg agtttgggag ccattttcgg aggaaaagcc tttgaacata
    15001 caaccaatag tgtggaacta gctattgata gtaatagtta tgcccacaat ctatcctatc
    15061 ttgtcttgaa caaggaatac ctgaaagggc atggtatcgg aagtagttat atcatggagt
    15121 tgtataccga ctatggtatg attggagtct ttctgcttag tttcttactc ggcgtattat
    15181 ttatagccat gctgcaagta gcctatcgct caaggacaat cctatttgct ttatccctac
    15241 tcatcttgaa taatctattc tttatgccaa gaagcagctt ttcagaaagt ttcttcaatt
    15301 tatttacaat gcaattctgg ggaattgttc ttgtgattat atttatagca aaaatgctta
    15361 caaaggaaaa ctagtatcta ctaaacaaag gagaaaaaaa tcatgtttga acattattca
    15421 gtagctgatt tgtttgcaaa tctttataaa aagcgaaaag caaatatttt agctctcatc
    15481 gctttatttg ctctcattgc tgtaccattt acaattaaag cagttaggaa taaaaacact
    15541 gtcaaagata caacaagtta ttcaacttat cttatctata aaatcactcc tccaaaagag
    15601 tcggccaaaa cgattttgaa tcatcaaatt ggtggttata gtgattttta tgggaaattg
    15661 attgatggta atttgaatgg agcttatctt ttcaatgatg tagaacccag tgagttgaaa
    15721 aaaattgcca gtgaattaga cacgacagaa acaaccttga aaaattcaac gaatgactat
    15781 tggtggaaaa aattgaccgt ctactatatg attgacgatg caggggttgg tgtgaaaatt
    15841 ttgacatcaa gtaaagatgc caatgacttg ttagagaaaa aaattgatgg gttgattgag
    15901 aaatttaaac atgcctatgc aaatgtgaaa attgaaaaac tggaaaccat caactctaaa
    15961 gaattgaacg caaatggtga aacagcgctt ggcttaaatg tgaaaaatct gattcttcgt
    16021 ttagttgtta ttggagtggt ttgtgtgatt ttggttgtga tgggaaatgt gttagtttat
    16081 ctctttaatc caacaatcaa tagagtaggt gatttttctc agtatcaaat tgattttgta
    16141 acagagatta caacaattgc taacctagca gatgttttgt catacaaaaa tactggacag
    16201 gaattgacca tcgttagctc aaataaagct atcctagata aattgaaaca gagtcaagaa
    16261 gctttaaaag gaatgcattt tgtagattta caggatgtat catctctttt ggaaagagat
    16321 acagtccttc ttgttgaaga gtacggagtg actcgttata agaaatttga gcaaagtctt
    16381 caaattctca gaaacttaaa tcgttctatc ctcggtgtag caacctttaa attataagta
    16441 ttctgatgta taaggtcttc aaagtcggct aagagccgat tttgaaggct ttttctatct
    16501 gtctgcagaa attttctttt gataaaatcg taaaaatcta gtataataga tagaactgaa
    16561 agtatgaggt tactagcaat gaaaatgaaa caaattagtg atacaacttt aaaaatcacg
    16621 atatctttag aggatttgat ggatcgtgga atggagattg ctgactttct cgttcctcaa
    16681 gaaaaaacag aagagttctt ttatgctatc ttggatgagc tagagatgcc tgatagcttt
    16741 ctggatacag gcatgttgag cttccgtgtg actccaaaac ctgataaggt agatgtcttt
    16801 gtgaccaagt caaagattga ccaaaatcta gattttgaag acttatcgga tttgccagat
    16861 atggaagaat tggctcaaat gtctccagat gaatttatca aaaccctgga aaaaagcatc
    16921 gcagacaaaa ccaaggatga tatcgaagcc attcaatctc ttgagcaagt tgaagccaag
    16981 gaagaagaac aagagcaggc tgaacaagaa gctgagagta agaaagaacc ttacatctac
    17041 tacatccttt cttttgctaa gttggctgac ttggtagctt ttgccaagac agtgactttt
    17101 gaaatggaaa cttctgaact ctacaaaatg aacgagcgct attatttgac cattttagtg
    17161 gatattgaaa atcatccaag cccatatcca gcttggctgt tggcccgtat gcgcgagttt
    17221 gcagaggata gtgatatcag tcgctcagtc ttacaagagt atggtcaagt tttgatgagt
    17281 cacgatgcag tgctcaatct gcaaaaaatc ggctaatctt ttctggaaag ctattttcag
    17341 attttaagaa gagcgaatcg tctgattcgt tttttctttt tatactgaaa tagtgattta
    17401 ctataatagg aattttcaca aaattctgtt ataatggcta tattagaaaa tttcgaggag
    17461 ataaagatga cagttaaaat tgctttacta ggatttggta ccgttgcaag tggtgtgcct
    17521 ttcctcctaa aggaaaatgg aggaaaaatc aatcaatcag cacattcaga tatcaaagtt
    17581 gctaaggtat tggtcaagga tgaagatgaa aaaaatcgct tgcttgcagc agggaatgac
    17641 tttaactttg taaccaatgt ggatgatatt ttatcagacc aggatattac tatcgtagtg
    17701 gaattgatgg ggcgtattga gcctgctaaa acctttatca ctcgtgcctt ggaagctgga
    17761 aaacacgttg ttactgctaa caaggacctt ttagctgtcc atggcgcaga attgctagaa
    17821 atcgctcaag ctaacaaggt agcactttac tacgaagcag cagttgctgg tgggattcca
    17881 attcttcgta ctttagcaaa ttccttggct tctgataaaa ttacgcgcgt gcttggagta
    17941 gtcaacggaa cttccaactt catggtgacc aagatggtgg aagaaggctg gtcttacgat
    18001 gatgctcttg cggaagcaca acgtctagga tttgcagaaa gcgatccgac gaatgacgta
    18061 gatgggattg atgcagccta caagatggtt attttgagcc aatttgcctt tggcatgaag
    18121 attgcctttg atgatgtagt ccacaaggga atccgcaata tcacaccaga agacgtagct
    18181 gtagctcaag agcttggtta cgtagtgaaa ttggttggtt ctattgagga aacttcttca
    18241 ggtattgctg cagaagtgac tccaaccttc ctacctaaag cgcacccact tgctagtgtg
    18301 aatggcgtaa tgaacgctgt ctttgtagaa tctatcggta ttggtgagtc tatgtactac
    18361 ggaccaggtg cgggtcaaaa accaactgca acaagtgttg tagctgatat tgtccgtatc
    18421 gttcgtcgtt tgaatgatgg tactattggc aaagacttca acgaatatag ccgtgacttg
    18481 gtcttggcaa atcctgaaga tgtcaaagca aactactatt tctcaatctt ggctccagac
    18541 tcaaaaggtc aggtcttgaa gttggctgaa atcttcaatg ctcaagatat ttcctttaag
    18601 caaatccttc aagatggcaa agagggtgac aaggcgcgtg tcgttatcat cacacacaag
    18661 attaataaag cccagcttga aaatgtctca gctgaattga agaaggtttc agaattcgac
    18721 ctcttgaata ccttcaaggt gctaggagaa taagatgaag attattgtac ctgcaaccag
    18781 tgccaatatc gggccaggtt ttgactcggt cggtgtagct gtaaccaagt atcttcaaat
    18841 tgaggtctgc gaagaacgag atgagtggct gattgaacac cagattggca aatggattcc
    18901 acatgatgag cgtaatctct tgctcaaaat cgctttacaa attgtaccag acttgcaacc
    18961 aagacgcttg aaaatgacca gtgatgtccc tttggcgcgc ggtttgggtt cttccagctc
    19021 ggttatcgtt gctgggattg aactagccaa ccaactgggt caactcaact tatcagacca
    19081 tgaaaaattg cagttagcga ccaagattga agggcatcct gacaatgtgg ctccagccat
    19141 ttatggtaat ctcgttattg caagttctgt tgaagggcaa gtctctgcta tcgtagcaga
    19201 ctttccagag tgtgattttc tagcttacat tccaaactat gaattacgta ctcgcgacag
    19261 ccgtagtgtc ttgcctaaaa aattgtctta taaggaagct gttgctgcaa gttctatcgc
    19321 caatgtagcg gttgctgcct tgttggcagg agacatggtg accgctgggc aagcaatcga
    19381 gggagacctc ttccatgagc gctatcgtca ggacttggta agagaatttg cgatgattaa
    19441 gcaagtgacc aaagaaaatg gggcctatgc aacctacctt tctggtgctg ggccgacagt
    19501 tatggttctg gcttctcatg acaagatgcc aacaattaag gcagaattgg aaaagcaacc
    19561 tttcaaagga aaactgcatg acttgagagt tgatacccaa ggtgtccgtg tagaagcaaa
    19621 ataaagaata gaagatagga tggggaaact cttgaccaga gggtttcata tcctttttgt
    19681 gaaaagaagt ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggctg
    19741 ctcaaaacag tgttttgagg ttgcagatag aactgacgaa gtcagtaaca tatatacggt
    19801 aaggtgacgc tgacgtggtt tgaagagatt ttcgaagagt attagttaaa aacgtgataa
    19861 aggagaaata aagatggcag aaatttatct agcaggtggt tgtttttggg gcctagagga
    19921 atatttttca cgcatttctg gagtgctaga aaccagtgtt ggctacgcta atggtcaagt
    19981 cgaaacgacc aattaccagt tgatcaagga aacagaccat gcagaaacgg tccaagtgat
    20041 ttacgatgag aaggaagtgt cactcagaga gattttactt tattatttcc gagttatcga
    20101 tcctctatct atcaatcaac aagggaatga ccgtggtcgc caatatcgaa ctgggattta
    20161 ttatcaggat gaagcagatt tgccagctat ctacacagtg gtacaggagc aggaacgcat
    20221 gctgggtcga aagattgcag tagaagtgga gcaattacgc cactacattc tggctgaaga
    20281 ctaccaccaa gattatctca ggaagaatcc ttcaggttac tgtcatatcg atgtgaccga
    20341 tgctgataag ccattgattg atgcagcaaa ctatgaaaag cctagtcaag aggtgttgaa
    20401 ggccagtcta tctgaagagt cttatcgtgt cacacaagaa gctgctacag aggctccatt
    20461 taccaatgcc tatgaccaaa cctttgaaga ggggatttat gtagatatta cgacaggtga
    20521 gccactcttt tttgccaagg ataagtttgc ttcaggttgt ggttggccaa gttttagccg
    20581 tccgatttcc aaagagttga ttcattatta caaggatctg agccatggaa tggagcgaat
    20641 tgaagttcgt tctcgttcag gcagtgctca cttgggtcat gttttcacag atggaccgcg
    20701 ggagttaggc ggcctccgtt actgtatcaa ttctgcttct ttacgctttg tggccaagga
    20761 tgagatggaa aaagcaggat atggctatct attgccttac ttaaacaaat aaaacagaga
    20821 gtggggcttc ccactttctt catttctaga atatgaatag aagggattta tgaaacacct
    20881 attatcttac ttcaaaccct acatcaagga atcaatttta gcccccttgt tcaagctgtt
    20941 agaagctgtt tttgagctct tggttcccat ggtgattgct gggattgttg accaatcttt
    21001 acctcaggga gatcaaggtc atctctggat gcagattggc ctgctcctta tctttgcagt
    21061 aattggcgtt ttagtggcct tgatagctca attttactca gcaaaggcag cagtaggttt
    21121 tgctaaggaa ttgacaaacg atctttatcg tcatattctt tccttgccca aggacagcag
    21181 agaccgtctg acaacatcta gtttggtcac tcgcttgact tcggatacct accagattca
    21241 gactggtatc aatcaattcc tgcgtctctt tttgcgagcg cccattatcg tttttggtgc
    21301 catttttatg gcttatcgaa tctcaactga gttgactttc tggttcttag tcatggttgc
    21361 cattttgacc attgtcattg tagggttatc tcgattggtc aatcctctct acagtagtct
    21421 cagaaagaaa acggaccaac tggttcagga aacgcgccag caattgcaag ggatgcgggt
    21481 tattcgtgct tttggtcaag aaaaacgaga gttacagatt tttcaaaccc ttaaccaagt
    21541 ttatgctaga ttacaagaaa agacaggttt ctggtctagt ttattaacac ctctgaccta
    21601 tctgattgtc aatggaactc ttctcgttat tatctggcaa ggctatattt caattcaagg
    21661 aggagtgctc agtcaaggtg ctctcattgc tcttatcaat tacctcttac agattttggt
    21721 ggaattggtc aagctagcca tgttgatcaa ttccctcaac cagtcctata tctcagtcaa
    21781 gcgaatcgag gaagtctttg ttgaggctcc agaggatatc cattcagagt tagaacaaaa
    21841 gcaagctacc agagataagg ttttacaagt ccaagaattg acttttacct atcctgatgc
    21901 ggcccagcct tctctgagat acatttcctt tgatatgact caaggacaaa ttctaggtat
    21961 catcggggga actggttctg gtaaatcaag cttggtgcaa ctcttacttg gactttatcc
    22021 agtagacaag gggaacattg acctttatca aaatggacgt agtcctctta atttggagca
    22081 gtggcggtct tggattgcct atgtacctca aaaggtcgaa ctctttaaag gaaccattcg
    22141 ttccaacttg actctaggtt tcaatcaaga agtatctgac caggaactct ggcaggcctt
    22201 ggagattgcg caagctaagg attttgtcag tgaaaaggaa ggactcttgg atgctctagt
    22261 tgaggcaggg gggcgaaatt tctcaggtgg acaaaaacaa agattgtcta tcgcccgagc
    22321 agtcttgcgc caagctccgt ttctcatcct agatgatgca acctcggcac tggataccat
    22381 tacagagtcc aagctcttga aagctattag agaaaatttt ccaaacacga gcttaatttt
    22441 gatctctcaa cgaacctcaa ctttacagat ggcggaccag attctcctct tggaaaaagg
    22501 tgagttgcta gctgttggca agcacgatga cttgatgaaa tccagccaag tctattgtga
    22561 aatcaatgca tcccaacatg gaaaggagga ctagaatgaa acgacaaact gtaaaccaga
    22621 cgctcaaacg tttagccgta gatttagcaa gccatccttt cctccttttc ctagcctttc
    22681 taggaactat tgctcaagtt ggcttatcaa tttacctacc tattctgatt gggcaggtca
    22741 ttgaccaagt cctagtggct ggttcatcac cagttttttg gcagattttt ctccagatgc
    22801 tcttggtggt aataggaaat actctggtac aatgggccaa tcctctcctc tataatcgtc
    22861 taatcttctc ttataccaga gatttacggg agcgaatcat ccataagctc catcgtttac
    22921 cgattgcctt tgtagatagg caaggtagtg gagagatggt tagtcgtgta accacggaca
    22981 tcgaacagtt ggcagctggc ttgaccatga tttttaacca atttttcatt ggtgttttga
    23041 tgattttggt cagtattcta gccatgctcc aaattcatct cctcatgact ctcttagtct
    23101 tgctgttgac gccactgtcc atggtgattt cacgctttat tgccaagaaa tcctatcatc
    23161 tcttccagaa gcaaacagag acgaggggaa ttcagactca gttgattgaa gaatcgctta
    23221 gtcagcagac tataatccag tccttcaatg ctcaaacaga atttatccaa agattgcgtg
    23281 aggctcatga caactactca ggctattctc agtcagccat cttttattct tcaacggtca
    23341 atccttcgac tcgctttgta aatgcactca tttatgccct tttagctgga gtaggagctt
    23401 atcgtatcat gatgggttca gccttgaccg tcggtcgttt agtgactttt ttgaactatg
    23461 ttcagcaata caccaagccc tttaacgata tttcttcagt gctagctgag ttgcaaagtg
    23521 ctctggcttg cgtagagcgt atctatggag tcttagatag ccctgaagtg gctgaaacag
    23581 gtaaggaagt cttgacgacc agtgaccaag ttaagggagc tatttccttt aaacatgtct
    23641 cttttggcta ccatcctgaa aaaattttga ttaaggactt gtctatcgat attccagctg
    23701 gtagtaaggt agccatcgtt ggtccgacag gtgctggaaa atcaactctt atcaatctcc
    23761 ttatgcgttt ttatcccatt agctcgggag atatcttgct ggatgggcaa tccatttatg
    23821 attatacacg agtatcattg agacagcagt ttggtatggt gcttcaagaa acctggctca
    23881 cacaagggac cattcatgat aatattgcct ttggcaatcc tgaagccagt cgagagcaag
    23941 taattgctgc tgccaaagca gctaatgcag actttttcat ccaacagttg ccacagggat
    24001 acgataccaa gttggaaaat gctggagaat ctctctctgt cggccaagct cagctcttga
    24061 ccatagcccg agtctttctg gctattccaa agattcttat cttagacgag gcaacttctt
    24121 ccattgatac acggacagaa gtgctggtac aggatgcctt tgcaaaactc atgaagggcc
    24181 gcacaagttt catcattgct caccgtttgt caaccattca ggatgcggat ttaattcttg
    24241 tcttagtaga tggtgatatt gttgaatatg gtaaccatca agaactcatg gatagaaagg
    24301 gtaagtatta ccaaatgcaa aaagctgcgg cttttagttc tgaataagcc attctctttt
    24361 gaaagtttat ggacgaaaaa agttgccttc gagtgacttt tttgttacaa tagctagaaa
    24421 aattgttcac tgtaatactc aatgaaaatc aaagagcaaa ctaggaagct agccgtaggt
    24481 tgctcaaagc acagctttga ggttgtagat aagactgacg aagtcagttc aaaacactgt
    24541 tttgaggttg cagatagaac tgacgaagtc agctcaaaac actgttttga ggttgcagat
    24601 agaactgacg aagtcagtaa ccacacctac ggcaaagtga atctgaagtg gtttgaagag
    24661 agtacaactt gtcttttaga aaaggagcct ataatgaaag tctttcagca tgtaaatatc
    24721 gtgacttgtg atcaagattt ccatgtttat cttgatggaa tcttagcagt caaggattct
    24781 caaatcgtct atgtcggtca agataagcca gcgtttttag agcaagctga gcagattata
    24841 gactatcagg gagcttggat tatgcctggt ttggtcaatt gtcacaccca ttctgcaatg
    24901 acaggtctga gagggatccg agatgacagc aatctccatg aatggctcaa tgactatatt
    24961 tggccagcag aatctgagtt tactcccgac atgactacca atgcggtcaa agaagccctg
    25021 acagagatgc tccagtcagg aacaacaacc tttaacgata tgtataatcc caatggtgtg
    25081 gatatccagc aaatttatca ggtggtgaaa acttccaaga tgcgttgtta tttttctccg
    25141 actctctttt cttcagagac agaaacaact gctgagacta taagtagaac tcgatccatc
    25201 atagacgaaa tcttaaaata taaaaatcca aatttcaagg ttatggtagc acctcattct
    25261 ccgtatagct gcagtagaga cttgctggaa gcgagtttgg aaatggcaaa agagctaaat
    25321 attccgctcc atgtccatgt ggcggagacc aaggaggagt caggaattat cctcaaacga
    25381 tacggcaaac gcccccttgc ttttcttgaa gaactgggtt atttagatca tccgtccgta
    25441 tttgctcacg gggtcgaatt aaacgagaga gaaattgaac gcttggcatc ttctcaagtg
    25501 gctatcgccc acaatcctat cagtaacctc aaactggcat caggaattgc tccaattatc
    25561 cagctccaaa aagcgggagt agcagtcgga attgcgactg actcggttgc ttccaataac
    25621 aatctagata tgtttgagga aggaaggact gcagctcttc ttcagaagat gaaaagtggg
    25681 gatgccagcc agtttccaat cgaaacagct ctcaaggtac tgacaatcga aggggctaag
    25741 gtccttggaa tggaaaatca gataggaagt ctggaagtcg gcaagcaagc agattttctg
    25801 gtcattcaac cacaagggaa aattcatctc caacctcagg aaaatatgct gtctcacctg
    25861 gtttatgcag ttaaatctag tgatgtagat gatgtttata tcgccggaga acaggttgtt
    25921 aagcaaggtc aagtcctgac agtagaactt taaaagaaaa attacgaaaa attttaaaaa
    25981 aagtttgcaa aaatcttgca ttcttttttt gactatgcta tacttatata cggtttaaaa
    26041 aaactgccta agacagtagg ggagctcgac tcataaatat cctaccgagg acaaaacgta
    26101 tcatgtaaaa agaagcgtat tgtactttcg tgtctaggtt tgggcgcgtt tttctttttg
    26161 aaaaattccc caagcaaaat aattacggag gtgaacacac taatgagtga agcaattatt
    26221 gctaaaaaag cggaactagt tgacgtagta gctgaaaaaa tgaaagctgc tgcatctatc
    26281 gtcgttgtag acgctcgtgg tttgacagtt gagcaagata cagttcttcg tcgtgagctt
    26341 cgtggaagcg aagttgagta taaagttatt aaaaactcaa tcttgcgtcg tgcagctgaa
    26401 aaagctggtc ttgaagatct tgcatctgta tttgttggac catctgcagt agcattttct
    26461 aatgaagatg ttatcgcacc agcgaaaatc ttgaacgact tttctaaaaa cgctgaagca
    26521 cttgaaatta aaggtggtgc aatcgaaggc gctgtcgcat ctaaagaaga gattcttgca
    26581 cttgcaactc ttccaaaccg cgaaggactt ctttctatgc tcctttctgt acttcaagcg
    26641 ccagtgcgca acgttgctct tgcagtcaaa gcggttgcag aaagcaaaga agacgcggct
    26701 taatcttaag ctacacagcg tagcttagct acgaaaaaaa ctattataaa atttaaaact
    26761 tatttggagg aaataacaat ggcattgaac attgaaaaca ttattgctga aattaaagaa
    26821 gcttcaatcc ttgaattgaa cgaccttgta aaagctatcg aagaagaatt tggtgtaact
    26881 gcagctgctc ctgtagctgt tgctgcagct gatgcagctg atgctggtgc tgctaaagat
    26941 tcattcgacg ttgaattgac atctgcaggc gacaaaaaag ttggcgttat caaagttgta
    27001 cgtgaaatca ctggtcttgg tcttaaagaa gctaaagaac ttgttgacgg tgcaccagca
    27061 cttgttaaag aaggcgttgc aactgcagaa gctgaagaaa tcaaagctaa attggaagaa
    27121 gctggagctt cagttactct taaataatag agtatcgcaa tttgattgcg gaatccctat
    27181 ttaccagtct ttgagagcga taagcgattg atagaaactg ataaattgat ttggtttcct
    27241 gccaaaaatt ctttggcagg atttttgttt taataaactt tcgggggtag acactagatt
    27301 gtctacccct ttttttgtgt ttcggaggta attatgcaaa aggaaggaca gtcatcacgt
    27361 caattcgtga agagtcatct catgactatc atgggagttg atattgtaaa agcaactcag
    27421 ttagttgatg aaatggaaca agtaggttta atccgatttg atgaatttgg aaatgttgga
    27481 attttagtct tggagggaca ataatgaagc gtattaccgc taatcaatat cagacatcag
    27541 agcgttatta taagctcccc aaacttttat ttgagagtga acggtataag aatatgaaac
    27601 tggaggttaa ggtggtttac tcagttttaa aagatcggtt ggagttgtct ttgagcaaag
    27661 gttggattga tgaggatggg gctatttatt tgatttattc caattcaaat ttgatggcac
    27721 ttttaggctg ttcaaagtca aaattactct ccatgtgagt ttgaagtgat atttttagat
    27781 gattaccata aaaaacataa ctacccacta ttttacgaat cctatcttca aaacgttatg
    27841 gaattccttg aaagtcaaga cataaagaat ggggttgatg cctttgtaga tgatcatcaa
    27901 aatctcgttt ttgttttata tggacaaggc tatcgagccg agggaaaaga gggaatactt
    27961 acaacccaag taactgtaaa agcttatgat gaagacaaga aaccgattaa cttcgcaaat
    28021 ttattagatt ccttaattta ttagattcct taatcgtgtc agaatatcaa atggaaccga
    28081 atctttggga ggtctcccat gattgatctc tatctaagta aaaatagaca aagaaatcaa
    28141 cttcttttag atttcttcca aaactatggc atagaggtat cttgtcattc agtttctgaa
    28201 atgacaaagg acaaattaat tgagatgatg agctattctt cagattgttt tgagttttta
    28261 tctcccaatt tattacgatt taagaaccgt gacaatctaa gattaacgga tttcgttgag
    28321 ataattttaa aaaatcctga actaaccatc agacttcctc ttgcggtttc aaataagcga
    28381 gtttatccaa gtctgaattt ggaagaggct agagctctat tgccaagaga tacgaaacaa
    28441 ttgatttaca tggcgcaaac acattattta tctaactaaa ggagaagcaa tatggctgaa
    28501 cgattttggg agaacttgtc cattattttg gctgaaagaa atatcagctg gattgagtta
    28561 accagaaaaa tgtttgcggg agagtttcac tatccaagtg aattgaatcg tttgtatcaa
    28621 aagattcgcc actagaagat ggaacaatga atgcctcaaa gtccatgggt agaaaggatt
    28681 gtgcaggtat tagatttaga ttatgaagat ttatttcgga ggtaactatg aaaagaataa
    28741 ttccagttta tatattccaa caagtaaatg tcctattggt atctctatac ttattgaaat
    28801 ttctttgtat cggtgagtta actatactac agattctcta tggttcgtca ctcatttctt
    28861 ttttatggat gtatggccaa cgcaaacaag cgcataaggt caatatgaaa tctaggatga
    28921 aatggcttgg tgttgaattt gttagcctac tgattataag tctatgtttt agtctgattc
    28981 atgctcaagg aagcacgaat caagcaaact taattggcct tcaacatcaa attccttggt
    29041 tttcattttt attgttctta atcaatgcga gtatggttga agaatttctg tatcgagaaa
    29101 ttttatggaa tttggtcaga aaattagata tcagagttgc tttgacaagt gttttatttg
    29161 ccttagcaca tcatccagga accattatag cttggtgttt gtatgtatcg ctggggatgt
    29221 ttttagggct tgtacgctac aaattggact tatggggcag tatggggcta catttggtgt
    29281 ggagcctact agtttatagt ttgctgcttt tttaaaatag gtaacctgat ttttaggtaa
    29341 cttctaaaac taacttccag ttttgggaga gagatggaag ttactttgaa aagttacgga
    29401 aacattattt tctacgtgac aacattgtat cattgcttgc attttactgc tggagagagt
    29461 ataatgaagg tggataaagc acttaatccc aaaaatttaa ataggaggag ttagctatgg
    29521 cagaagaagt attaaatctt caattagtat ctgttcaagt tgacgaaaca gatgaagttg
    29581 atggaatgcg atttagtaca ttcagtacaa accgttgcgg taactggagt gcttttagtt
    29641 gggaaaattg ttaattcatt taaattgtaa actttaggga ttctctggag ttatggatct
    29701 cgttccaata tgtgcacgtt ggaatgtcag tgcttatatg gggggaatcc aaaggattct
    29761 ccctttttat tttaataagt ctgtctagct atagatatat taagtttgat agaatttctt
    29821 ggagattttg ttatggatta taatttcaat ttggagcatc ctttcttttt cactaataat
    29881 gattatagta cagatactag cataaagtac taagtaagtc tgccttttaa ttggcacgaa
    29941 gtaatgaata atgatgagtg ggtttatcaa taccctattg gaaaatttgt tgaaagacaa
    30001 gggtggaaaa tacacattag ttcagagtat aatagttcac acgaattgtt acaagatgta
    30061 gcaaaaattt gccatgaaat gcgaattcca tttaaacatc ttagtacaga agacaaattt
    30121 atcatgcgaa acgggaaatt ggttagtcga ggattttctg gtaaatttat tacatgttat
    30181 ccaaatcaaa atgaattaga gtcggttttg caaaggttag aaagtgcctt aaagcaatat
    30241 aatgggccat atattttaag cgataaacgt tgggatgaag caccaatata tctgaggtat
    30301 ggtgtgttta ggccgtcaag ggatgatgaa aagaaggttg ttattgatga attaattgtt
    30361 ggcgatgaag ttgttaaaga tgaacgttta ccagtattta aaattccaaa aggtatagtg
    30421 ccccctgact ttttgaataa atggcttgac aagaaagata aaaaacaggg agactttccc
    30481 tttattatag ataatgcaat tagattttca aacagtggtg ggatatataa tgctagacta
    30541 aaagaagatg ggaaaaaaat aattttaaaa gaggctagac cgtatacagg tctaggattt
    30601 gatggtactt attcttcaga gagattagct tcggagtgca aagcactaaa aattctaaat
    30661 gaatggagtg agatgcctaa aatatactgg tacggtaaga tctgggagca caccttctta
    30721 ggaattgaac atatgaaggg cgttccacta aatagatggg ttaccaataa ttttccttta
    30781 tatgaagttg ttgataagac caaagattat ctattgagag tttcaaagat agtagaaaaa
    30841 ctcatcgatt taacgaataa gtttcattct gaaaatgtat atcatcaaga tttacattta
    30901 ggaaatatct tggttaagga tgaagatgag attagtataa tagactggga acaggctgtc
    30961 ttttcgaatg atgaaaaagt tgtacataaa gttgcagccc ctggttttag agcatggcga
    31021 gaaacacttc ccagtgagat agattggtat ggtattagac aaatagctca ttatttatac
    31081 atgccactag taactacatc ggatttgaca tacaactatg taagtcaaac caggatagaa
    31141 ggaaaaaaac tatttgagtc tttgggttat acaagagaac atatagatta tgtagaaagt
    31201 ttattgtcat atctagattc taaatgtcca cagattgaga atatttccag gaaaaaagtt
    31261 ttaaaaccca tgcatgagat tagaactatc gagagtgagc aagatataca agattttatt
    31321 ataaaagctt ttcttttagt gaactgatag gtattttctt agcaaacgat aaagttttag
    31381 ggccgataca atcaattgcc tattcattaa ataagataaa tacaaccaaa gatttaagga
    31441 aaccgttttt aaaatactta agtggagaga agaattttat agacgctgaa catgataata
    31501 acggactgta tacttcatca atagatgtga tacacatgaa aga
//
LOCUS       DAVLRT010000017        29543 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLRT010000017 DAVLRT010000000
VERSION     DAVLRT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.017"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..541
                     /locus_tag="VXM85_000765"
     CDS             <1..541
                     /locus_tag="VXM85_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345386.1"
                     /translation="SKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARA
                     LINKPAVLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEASLLCDYVAL
                     LNKGKLVEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHS
                     CEPTLEDIFITLTGGKLNA"
     gene            534..1247
                     /locus_tag="VXM85_000766"
     CDS             534..1247
                     /locus_tag="VXM85_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345387.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFCKYLMETQG
                     KVNDQQELVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(1569..1661)
                     /locus_tag="VXM85_000767"
                     /pseudo
     CDS             complement(1569..1661)
                     /locus_tag="VXM85_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1982..2683
                     /gene="pyrF"
                     /locus_tag="VXM85_000768"
     CDS             1982..2683
                     /gene="pyrF"
                     /locus_tag="VXM85_000768"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1345388.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QLIKQATNPDFICLTPGIRPQGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            2717..3349
                     /locus_tag="VXM85_000769"
     CDS             2717..3349
                     /locus_tag="VXM85_000769"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1345389.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            3529..3932
                     /locus_tag="VXM85_000770"
                     /pseudo
     CDS             3529..3932
                     /locus_tag="VXM85_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4028..4792
                     /locus_tag="VXM85_000771"
     CDS             4028..4792
                     /locus_tag="VXM85_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345390.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMVVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            4792..5544
                     /locus_tag="VXM85_000772"
     CDS             4792..5544
                     /locus_tag="VXM85_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345391.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            5554..6252
                     /locus_tag="VXM85_000773"
     CDS             5554..6252
                     /locus_tag="VXM85_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345392.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLANWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            6249..6896
                     /locus_tag="VXM85_000774"
     CDS             6249..6896
                     /locus_tag="VXM85_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345393.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(6976..7848)
                     /locus_tag="VXM85_000775"
     CDS             complement(6976..7848)
                     /locus_tag="VXM85_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1345394.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLARKTSSTRPMKKHFTQPTVTLLK
                     QMTWLLKVEKLISH"
     gene            complement(7860..8618)
                     /locus_tag="VXM85_000776"
     CDS             complement(7860..8618)
                     /locus_tag="VXM85_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1345395.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGASGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRTLLMHPE
                     IILFDEVTASLDSEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAHAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            complement(8619..9536)
                     /locus_tag="VXM85_000777"
     CDS             complement(8619..9536)
                     /locus_tag="VXM85_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275026.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1345396.1"
                     /translation="MYIAVWGSLGSFLLGLIVSIIRHYRIPVLAQVATAYIELSRNTP
                     LLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSAMQDSGIQVLFQGNNLLRILQGL
                     GVTIGISILSVLLSMMFGTVMGIIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFG
                     LARNFNINISGETSAIIVFTLWGTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYY
                     HIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFW
                     IYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            9569..9715
                     /locus_tag="VXM85_000778"
     CDS             9569..9715
                     /locus_tag="VXM85_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1345397.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            9843..11333
                     /gene="lysS"
                     /locus_tag="VXM85_000779"
     CDS             9843..11333
                     /gene="lysS"
                     /locus_tag="VXM85_000779"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1345398.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            11958..13094
                     /gene="lctO"
                     /locus_tag="VXM85_000780"
     CDS             11958..13094
                     /gene="lctO"
                     /locus_tag="VXM85_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1345399.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      13346..13434
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13649..14317
                     /locus_tag="VXM85_000781"
     CDS             13649..14317
                     /locus_tag="VXM85_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1345400.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            14599..15381
                     /gene="thiM"
                     /locus_tag="VXM85_000782"
     CDS             14599..15381
                     /gene="thiM"
                     /locus_tag="VXM85_000782"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1345401.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPV
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKKFKRVK"
     gene            15383..16012
                     /locus_tag="VXM85_000783"
     CDS             15383..16012
                     /locus_tag="VXM85_000783"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1345402.1"
                     /translation="MFHKELLKLYFICGTTTCQGKDLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      16221..16309
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16571..17131
                     /locus_tag="VXM85_000784"
     CDS             16571..17131
                     /locus_tag="VXM85_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1345403.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            17132..18517
                     /locus_tag="VXM85_000785"
     CDS             17132..18517
                     /locus_tag="VXM85_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345404.1"
                     /translation="MGLELRAIQSPIFPEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSPPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTEVASKEVASSPELFHMDRTTCEFGNRSLFSIADFTFYQGISCILGDNGVGKSTF
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRDFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            18519..19169
                     /locus_tag="VXM85_000786"
     CDS             18519..19169
                     /locus_tag="VXM85_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1345405.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCVLFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            19180..19872
                     /gene="tenA"
                     /locus_tag="VXM85_000787"
     CDS             19180..19872
                     /gene="tenA"
                     /locus_tag="VXM85_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1345406.1"
                     /translation="MEFTDIAMELSKKAWQVSFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            19877..20401
                     /gene="thiW"
                     /locus_tag="VXM85_000788"
     CDS             19877..20401
                     /gene="thiW"
                     /locus_tag="VXM85_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1345407.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAIIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            20401..21204
                     /locus_tag="VXM85_000789"
     CDS             20401..21204
                     /locus_tag="VXM85_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1345408.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYVAQVCLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            21197..21829
                     /locus_tag="VXM85_000790"
     CDS             21197..21829
                     /locus_tag="VXM85_000790"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1345409.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITVAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLIGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(22039..22830)
                     /gene="thiD"
                     /locus_tag="VXM85_000791"
     CDS             complement(22039..22830)
                     /gene="thiD"
                     /locus_tag="VXM85_000791"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1345410.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(22914..23012)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            23196..23591
                     /locus_tag="VXM85_000792"
     CDS             23196..23591
                     /locus_tag="VXM85_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1345411.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            23602..23973
                     /locus_tag="VXM85_000793"
     CDS             23602..23973
                     /locus_tag="VXM85_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1345412.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            23983..26226
                     /locus_tag="VXM85_000794"
     CDS             23983..26226
                     /locus_tag="VXM85_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1345413.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPTHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWIISIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            26433..28208
                     /gene="spxB"
                     /locus_tag="VXM85_000795"
     CDS             26433..28208
                     /gene="spxB"
                     /locus_tag="VXM85_000795"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1345414.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            28319..28666
                     /locus_tag="VXM85_000796"
     CDS             28319..28666
                     /locus_tag="VXM85_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1345415.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(28783..29543)
                     /locus_tag="VXM85_000797"
                     /pseudo
     CDS             complement(28783..29543)
                     /locus_tag="VXM85_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         8775 a         5290 c         6312 g         9166 t
ORIGIN      
        1 tagtaaattt tataatatta gtaaatcacg tgttgataat ttgttaaagc gagtaggatt
       61 atatgatagt cgcaagatgg tagcaggaaa attatccact ggaatgaggc aacgaatgct
      121 tttagcacga gctcttatca acaaacccgc tgtactcttt ctggatgaac cgacctcagg
      181 tctagatccc acaacttctc gaacaattca tgagttaatt ttagaattga aaacagcagg
      241 gacaacgatt tttctaacga ctcatgatat gaatgaagca agtcttttat gtgattatgt
      301 tgccttatta aataaaggga aattagttga gcaaggagct ccttctgaac tcattcaaag
      361 atataataaa gataaaaaga ttaaggttac agattataat gggaatcaga taacttttga
      421 ttttacatca ctagaacagg tatctcaggc tgatctggaa aatatttttt caattcattc
      481 atgtgagcct actttagaag atatttttat cacattaaca ggaggaaagc taaatgctta
      541 aacggtttct ggctttggta tggttgcgtt gtcaaattat cctttccaat aagagtattt
      601 tattgcaagt tttagtgcct tttgctttca catatttttg taaatatctt atggaaacac
      661 aggggaaggt caacgatcaa caggaattag ttcttttgat gatgtgttta cctttttctt
      721 tggctttggc tgttggaagt cctataacta ttatcttgtc tgaagaaaaa gaaaagtaca
      781 atttacaaac tcttctgttg agtggtgtta aaggatccga atacatttta tcaacgatgt
      841 ttcttccttt tttgctaact tttgtgatta tgggaactac tcctcttatt ttaggagtta
      901 caattgtaca tacttttaat tatattacaa tcgttcttct aacctcttta tccatcattt
      961 tattctattt attgataggt ttaaccgcga agagccaagt agtagctcag gttatcagtc
     1021 ttcctgctat gattttagtt gctttcttac cgatgctatc tggtttggat aagacagttg
     1081 cgaagataac agattatagt tttatgggac tgtttactaa gtttttcaca aaatgggagg
     1141 gattttcatg gaatgaaact ctaattccta atctaacact acttatttgg attgttcttc
     1201 tattaacttt aattacgata actattagga aaaagaaaat ttcttaattg agttatttta
     1261 atgattataa acacaagtgg gaaggaaaaa tgaactgatc tttttgacag caattctaca
     1321 gaatagtctt attgctatat tttgatttga gtgtacgaaa aaagaaaaat aacaatagtg
     1381 ctcatactaa ttgcagaagt tttgggtgat aagataactg ataaattgca ataaaaaatg
     1441 caacattttt aaatctcctc tataagtgct tcaaaacctg tcttgtaatc caagtatttt
     1501 tgggaacggt gattaataag ctagcaaagc accattaagg attttttcgg taattgttgc
     1561 caaatcggtt taagaaaata ctcacgaaga agtccattcg cattctcatt acttcccctt
     1621 tgccaagatg aataggcatc cgcaaaataa aacagaattc ccatttgttc aattaaaggg
     1681 taacaagcaa actctttttc tctgtcctca gtgaaagtct ttaactattc ttttggaaag
     1741 agtcttgtga ggtgttcaat agcagtcaac acatacgtga ccatgttttt acttgacaag
     1801 tgctagtaga aataatagaa taatagaaaa cctttaaagc agtccagaga ggcagctaag
     1861 gttagacggt gaaagggtgg agactaccca tttttcgtgg aaccttgctg ttggcaggtt
     1921 ccttttttcg tggcttctgt tggccagact ctctcactag taaaggtaaa aggagaaacc
     1981 tatgcgagaa catcgtccaa tcattgctct tgattttcct agttttgagg cggtcaagga
     2041 atttttagct cttttcccag cagaagaaag cctttatctc aaggtaggga tggagcttta
     2101 ttacgcagcg gggcctgaga ttgtgtccta cttaaaaggt ttgggtcata gtgtcttttt
     2161 ggatctcaaa cttcatgaca ttcctaatac agtcaagtca gccatgaagg tcttgtctca
     2221 gcttggtgtc gatatgacca atgttcatgc ggctggtggt gtagagatga tgaaggctgc
     2281 gcgtgaaggt cttggaagtc aagcaaaatt gatcgctgta acgcagctga catcaacatc
     2341 agaagcccag atgcaggagt ttcaaaatat ccaaacaagc ctacaagagt ctgtgatcca
     2401 ctatgccaag aagacagctg aagcaggctt ggatggtgtc gtttgctcgg cacaggaagt
     2461 tcaacttatc aagcaggcca ccaatccaga ttttatctgt ctgacaccag ggattcgccc
     2521 acagggagct gctgttggag accaaaaacg cgtcatgacg ccagcagatg cttatcaaat
     2581 tggtagtgac tatatcgtag tgggacgtcc cattacccaa gctgaggatc ctgttgcagc
     2641 ttatcatgcc atcaaggatg aatggacaca ggactggaat taaagaacta gattagaaaa
     2701 ataaaaggag aataccatga cacttgctaa agatatcgct agccacctct tgaaaatcca
     2761 agccgtttac ctcaaaccag aggaaccctt cacttgggcg tctggtatca agtcaccgat
     2821 ttacactgat aatcgtgtga cactagccta tccagaaact cgtaccctaa ttgaaaatgg
     2881 ttttgtggaa gctatcaaag aagcctttcc tgaagtagaa gtgattgcag gaactgcaac
     2941 agcagggatt ccacacggag ccattattgc tgataagatg gacttgcctt ttgcctacat
     3001 ccgtagcaaa ccaaaagacc acggagctgg taatcaaatc gaaggtcgcg tagctcaagg
     3061 tcaaaaaatg gtagtggttg aagaccttat ttcaacgggt ggttcagttc ttgaagctgt
     3121 agcagcagcc aagcgagaag gagcagatgt acttggagtt gtagcgattt tcagctacca
     3181 attgccaaaa gcagataaga actttgcaga tgctggtgtt aaacttgtga cgctttcaaa
     3241 ctatagcgag cttatccatc tagcccaaga agaaggttac atcacgccag agggccttga
     3301 tcttctaaaa cgctttaaag aagaccaaga aaattggcaa gaaggttagg tcagtaagat
     3361 aaagagagac gaggctaccg agtctctttt accattttat ttaaaatatg ataatatttt
     3421 aaataaaata attgacaacg ctttctttta ttgatataat gaaaatgtaa tatgtatcaa
     3481 attgatcaat caactatctt gtttatcact tcttaagagg acgatgtcat gaaaagaaga
     3541 aaaatatatt ccttttttgc gtagttacaa ttgcatctag tttgtttctc cctacagttt
     3601 tagctagaca gattggaaat tatgatttaa cgttgccgcg ttggggttcg gatacaacta
     3661 gtgagcttga gaaagaaaac gcctctgctg gaattaataa tagtgacagc actggtggcg
     3721 gtaaaaggtt aaatacctct attcgtagcg cctatagtgg gtcagatatt accccggtat
     3781 attcattggg gtctggctct aggattgtca tgtactataa tggaggtggt gacaattata
     3841 ttggttctgg tactagatta gctatggcgc cacaatttgg aaatcatgta agaattcata
     3901 cttcaggttc ttggagtcca gattcttatt aacttacttg tcagagtaag ccttaaagat
     3961 ggttgattgt gggtgtagca tgaaaaaaga atgctacacc ctatttttat tataaggagg
     4021 agtaaggatg gaatttttca tttgtaatct tgtacgagtc gttcaatcac ctcggtttta
     4081 tatgtcttta tttttgaccc ttctttgcat gagtttagga aatttccttg ctttcaatgg
     4141 tatttataaa attgaaggtt tatcgatttt ttttgccgct tcttctattc gaggattttc
     4201 accgattagc ctagtagctg cacttatctg tacactgccc tattctagtc agataataga
     4261 ggatgctgag agtcattttc taacagcaca attgtgccga atttctaaaa agaggtatct
     4321 agctattgta ggtagtactg caattatttc ttcttttcta gtcttttttc tcccctattt
     4381 attattatta ggaattaatc ttttagtgac tccttatcag gaaatttata ttggagatta
     4441 tagtggtgcc ttaaaagaat tatttgattc taatcagttt ctctatagtc ttgtgacgac
     4501 tctctggtat ggagtttggg gcgctgtgtt ctctattttt ggactagcta gtgctttgct
     4561 agtgaagaaa aaattaggag ctattttcat cccagttgcc tatatggtgg ttggtggtat
     4621 tttatgggct attttagggc tatcttactt agaacctgtg acaacgctag ctttgggata
     4681 tcagaaagat atcagtcttt ccttagttag tgggcatctt gcttttattt tatttgttag
     4741 ttgtttggtt gtttatggta cattttttct acattcagag gactatgtat aatgaaacaa
     4801 tttgttcaat tttataaaaa agatttctta gcagtattgg tttattttat attactgcta
     4861 tcctgtgttt tatctagtac agtatattta ttgcgctgtc gccaatattc aatccatcca
     4921 aatgtattag aatggatttt agttttactt caagatatga cgactggagt atattgcttt
     4981 ccgttcacat atatattgtt ctttttttat ttgatgaata actattttaa taggttggag
     5041 tgtcgcattc gtctgaaatc aattaagcac tttaccagtt ttagtttcaa attagcagct
     5101 cttagtacgg ggatttggac ggcgacttta tttttattga tttttctaat tgcatttagt
     5161 aatggtttta gcttctcttt ggagataaag gaggttgatt ttttaagaga attttatggt
     5221 ataagtattg caaacaatgc tagtttcttt ataggatttt ttttctctta tatagcatac
     5281 tatttctttt tatccttact tactattagc agtttttctt ggtttaaaaa atcaaacatg
     5341 agcttagtat ttctgtttac ttttttattt gtagaatcct tattctggat ttatcagttg
     5401 aacaatggga taattggatt attgccaatt tttcagtata tggtaaattc caatccgtat
     5461 gcattgattt attggcttac attactatct atcataattc cattgactgt attttctgtt
     5521 catagaaact ggaggagagt gtaaaagttg gaaatgggaa agttaagtag tcacatgtgg
     5581 aggttgaatc agataatcta taccaagtac ttttggggtt atgttctttt ttggatattg
     5641 atttgtttag gattatggta ttggttagaa ggaaatgata gacttgttat agaaatttta
     5701 aaagggccta atctgagtca aaactctttt ttagtcttat ctatatggtt gcttcattgg
     5761 tttattattc atacattttt tctagcagtt gtatatcgta gaagagcatc cgatttcttt
     5821 atggaagtga ttcgattttc ttctattaag ctctggatta ggtatcagat ttggacctgt
     5881 tttctttatg gactcatttt aatcatggta aaagttctag tgattcaatt tatgttacag
     5941 ttagcaaact gggatatagg agttttgttt atagttgatt ctttgaatgc ttgtgtgcta
     6001 tccttgcttt gctttatgtt atacgcacta ggagcgaatg tacaaatgaa ctttgcttgc
     6061 gttagtttct ttttactcat gattgtgttt ggtggtctat ttgtaggaaa tcgaactaat
     6121 tatctatttt atattttgaa tcgagggaat ggagatatag gacgagattt atttcttcag
     6181 ttgctatttt tagtctttct ttttaaaagc attttctatt tcactcgtca aaagaggagg
     6241 tttatagaat gaatgaaata attacattaa aaaatattga gttgaaatta aaaaaaacat
     6301 gtgtttttca aaaccttaat tttagttgta aacaggggga aattatagga attactggtg
     6361 cgaatggctc aggtaaaagt gtattgttta aattaatagc tggtttatat agtccgtctt
     6421 atggagaagt gttaatcaat ggggaaaata ttgttcctga gagaaaaatt ccagctaatt
     6481 tgggagcttt gattgaagaa cctggtttta taaattatta tagtggcttt aagaatttac
     6541 aatatttggc aagcatacga ggagtagttg gtaatcagga aatcaatgat acactgaaaa
     6601 tagttggtct atatgagcaa aaagaccaga aagttaaaac ttattcgcta ggtatgagga
     6661 aaaagctagg gattgctcaa gcaattatgg agaatccctc tattctttta ctagatgaac
     6721 ctatgaatgc cttggataaa tcaagtgtag aaaatatgag aacattgttt agaaagctct
     6781 ctagtgaaaa agggacaaca attttgattg ctagtcatag tgaagaggat attcgtatct
     6841 tatgtgataa agtatatgca atagaagata aagtatgtac actgtgttca gattgaacat
     6901 cttagataga ctcaggatgt tataggtaaa atcttaaaaa gggattggag cttaaaatcc
     6961 agttcctttt aagagttaat gactaatcaa cttttccacc ttcaacaacc aggtcatctg
     7021 ctttagcagt gtcaccgtgg gttgggtgaa gtgttttttc ataggccttg tggaagaagt
     7081 tttccttgct aatttttcaa tatctttatt gatgaagtct agcaattctt ggttgccttt
     7141 ttgaactgct gccgcaatgg tatcgggatc accgagggaa gtaattccta cttcaaatcc
     7201 tttattttca agcgcccaag ctagaacttc cgtattgtca gttgaaaagg catctccacg
     7261 tccgtcaaga agagcttggt aagagtcact gtattggtcg tatttttgga gtttgatttc
     7321 tggatgattc ttttcaaaat aagtctcagc agtcgttcct tttgtgacaa ttaaggtttt
     7381 accttcaagt tgtttgacgt ctgtaatgag accagtctta ggtgatacga cacccagaga
     7441 aactttcata tatggaaggg caaaatcaac ttgtttctta cgttcgtcag ttactgtaaa
     7501 gttagcaaga gtaatatcta ccttgtttga aatcaagtat tccgcacggt tggcagcatc
     7561 gactgaaatg tatttaacct tgacaccaag gtcttgagct agttggttcc ctagttcaat
     7621 atcgtagcct tggtaagaac catcattgtc aacgtagcca aacggttttt tatctccaaa
     7681 cacggcgatt cgcagttcac cgcttttttt gatttcatcg atagtgcgag ctttggcagt
     7741 tgtttttcca gaggatgaac cagcgtttcc acctgagcta caagcagtga taaagataag
     7801 ggcaaaggca agtgctaaaa cagttaagag tggtttgaat agtttcataa gaatctcctt
     7861 tatagatatg agccaaattg gctaaagtca aagacgttta aaaattcctg ggctcgtttg
     7921 gtttgcggat tggtaaagaa ggcatgagct gttccttctt cagcgatttt cccttggtcg
     7981 aggaagataa tccggtcagc aatggcttgg gcaaactgca tttcgtgggt tactaaaatc
     8041 atggtacggc cttcttgggc caaatcattg ataagttcca gcacctcacg caccatttct
     8101 gaatccagcg aagcagtcac ctcgtcaaaa aggatgattt ctggatgcat taggagggta
     8161 cggacaattg caacacgttg cttctgtcca ccagataatt gacgggcaaa gctatgttgt
     8221 ttatccagca aaccgacacg atctagtaat tgcaaagctt cttccgttac ttctttcttg
     8281 tcccttcctt gagttttgat agggcctagg atgaggtttt gtaagacatc cagatgggga
     8341 aagagttcat aactttgaaa gaccatgcca atcttttggc gaactaggtg aaaatctttt
     8401 ttattttcaa cgatagactg accatccaga aaaatatctc caccttgaat actttctaag
     8461 ccgttgaggc aacgaaggag ggtacttttc ccacaaccag atgcccctag gatgacaaca
     8521 acttcccctt ttttaatttc tagagaaagt ccttggagga tgggattgtc tccgaaggat
     8581 ttttttagtt ccttgatttc taagatagtt tcagacattt agtttctcca atgtttttct
     8641 aagtgagtgg atagtttgga aatagggtag caaactgcga aatataagac tagaatggtt
     8701 ccataaatcc aaaatgaagc agttgggatg gtcaggcgat tgctatcgat gatttgttgt
     8761 ccaactttgg tcacttccac aaccccaatc aaaacaacta atgaagtggt tttaatcatc
     8821 cgagtgacaa gattgatagc ctgcggtagc agtcttctca agacttgtgg gatgatgatg
     8881 tggtagtaaa gttgaacatt agtcaagccg agtgcttgtc cactttcaaa ctgatgttta
     8941 gggagagaag tgatagctcc acgtaccaag tctcccattt cagctgttcc ccagagggta
     9001 aaaacgataa tagctgaagt ctcacctgag atattgatat taaagtttcg agccaagcca
     9061 aagtaaacga tgaagagtag caccagctgg ggcatgatac gggtaaagtc cagatacaat
     9121 cgtgttaaaa atcgtatgat tctagaatgg gaggtcatga tgattcccat gactgttccg
     9181 aacatcatgg ataagaggac agacaggatg gatatcccaa tcgtaacgcc caatccctgt
     9241 aagattctca ggagattatt tccttgaaag agtacttgga ttcccgaatc ctgcatggcg
     9301 gagcctccta aaaagacaag cccaagcgtt gcacagactt ctgaagatag gacaatcccg
     9361 attcggggaa gaccgaagta gagaaagaag agttgaatca aaaggggcgt attacgtgac
     9421 aattcaatgt aggctgtcgc tacttgcgcc aaaacaggga ttcgataatg tcggatgata
     9481 ctaacgatta aaccgagcag aaaggatccc aaacttcccc aaactgcaat atacaaagta
     9541 aacgcttact tatcaaggag tgcattttat gtcatacaaa acaagcaatg cagaaggtca
     9601 tgtagatttc atcaatacct atgatttgga gccaatggcg caacaagtta ttcctaaagc
     9661 agcatttggc tatatcgcta gtggggcgga agatactttc acttctttcc agtgatttta
     9721 gcgtcaggtt ctttttagtt tttaaagatt atccgtgaat ttcttgctta tttatgataa
     9781 aatgggagta tcgcaaaaaa tgactcatcg tattcaattt tgagtaaaac taggaggatc
     9841 ccatgtctac agaacatatg gaagaactaa atgaccagca gatcgttcgc cgtgaaaaaa
     9901 tggctgcgct ccgcgaacaa ggaatcgatc ctttcggaaa acgttttgaa cgtactgcaa
     9961 attcacaaga attaaaagat aaatatgcca acctcgataa agaacaatta cacgataaaa
    10021 acgaaacagc tactatcgca ggacgcttgg taaccaaacg tggtaaagga aaagttggtt
    10081 ttgcccacct tcaagaccgc gaaggccaga ttcagatcta tgttcgtaag gatgctgtcg
    10141 gtgaagaaaa ctacgaaatc ttcaaaaaag cagaccttgg tgacttcctt ggtgtcgaag
    10201 gtgaagtgat gcgtacggat atgggagaac tctctatcaa ggcaacccac atcacacact
    10261 tgtctaaggc tcttcgtcct cttcctgaga aattccatgg tttgacagac gttgaaacaa
    10321 tttaccgtaa acgttacctt gacttgattt ctaatcgtga aagctttgaa cgctttgtca
    10381 ctcgttcaaa aatcatctct gaaatccgtc gttaccttga ccaaaaagga ttccttgaag
    10441 tggaaacacc tgttcttcat aatgaagccg gtggtgctgc tgcccgtcca tttatcaccc
    10501 accacaatgc ccaaaacatt gacatggtgc ttcgtatcgc gactgagctt cacttaaaac
    10561 gccttattgt gggtggtatg gaacgtgtct atgaaattgg ccgtatcttc cgtaacgaag
    10621 gaatggacgc tactcataac cctgagttca cttctatcga agtttaccaa gcttatgcag
    10681 acttccaaga tatcatggac ttgacggaag gcattatcca acatgctgct aaatcagtca
    10741 aaggcgatgg cccagtcaac taccaaggta ctgaaatcaa gattaacgaa ccatttaagc
    10801 gtgttcatat ggtggatgct atcagagaaa ttactggtgt cgatttctgg caagacatga
    10861 ctttggaaga agctaaaact atcgctgctg agaagaaagt tccagttgag aaacactaca
    10921 ctgaggttgg tcacatcatc aatgccttct ttgaagagtt tgttgaagaa actttaatcc
    10981 aaccaacctt tgtctatgga catccagtag ctgtatctcc actcgctaag aaaaatcctg
    11041 aagaccaacg ctttactgac cgtttcgagc tctttatcat gactaaggag tacggtaatg
    11101 cctttactga gttgaacgac ccaatcgacc aacttagccg ttttgaagcc caagctaaag
    11161 ccaaagaact tggtgatgat gaagcgacag gaatcgacta tgactacatt gaagctcttg
    11221 aatacggtat gccaccaaca ggtggtttgg gaatcggtat cgaccgtctc tgcatgctcc
    11281 tcactgatac aacaactatc cgtgatgtat tgctcttccc aacaatgaaa taaattctta
    11341 tcctctggct cttaccaggg gattttttga ttcaaaaaga gactgaattt aaggagaaaa
    11401 tgaagtgtag tatattgaag ttgaaatagt acactttgat ttctaagaca ttgttagaaa
    11461 ttggtttaaa ttccctaagc aatttgtgca tgttttattt cattttacga tagtacgctg
    11521 aaactgttca aaaagtacta gaaattgact tggattcccc aattgatttg ttcagattca
    11581 ctataaataa aaaattaata agtgggatag gaagttagcg tcaactagga tagtatcttg
    11641 cttaaacagt atatatggga ctgatataag tccataggtc ctattagagg atgttctggt
    11701 gtcttattca cttgtttttt tatagtatta gtagatagaa tccgcaaata aaaaagtagt
    11761 tcaaatgcct ggcagcaaaa aatctcaacg attcacttaa tatagactaa agtttagagt
    11821 ttaaggaaag tagggctagt atattgtatg aaaaaataag atggttttta agcaaaactc
    11881 ttgaaaatga ttgaaaaaag agttattata gttgtgtagt aaaaagcaaa cgcttactta
    11941 atcaaggagg gcattttatg tcatacaaaa caagcaatgc agaaggtcat gtagacttca
    12001 tcaataccta tgatttggag ccaatggcgc aacaagttat tcctaaagca gcatttggct
    12061 atatcgctag tggggcggaa gatactttca ctttaagaga gaatatccgc gcctttaacc
    12121 acaaactcat cgttcctcat acactttgcg atgttgaaaa tccaagtaca gagattgaat
    12181 ttgcaggtga aaaactatct tcaccaatca ttatggcgcc tgttgcggct cataaattgg
    12241 caaatgaaca gggggaagtg gcgactgcgc gtggtgtgca tgagtttggt tctctttata
    12301 caaccagctc ttactctact gttgaccttc cagagatttc tgaagccctt caagggacac
    12361 cccattggtt ccaattttac tttagtaagg atgacggtat caaccgccac atcatggacc
    12421 gtgtgaaggc tgaaggttat aaagcgattg tcttgacggc agatgctact gtagggggga
    12481 atcgtgaagt ggataagcgt aatggttttg tcttcccagt tggcatgccg attgttgaag
    12541 aatacctgcc agaaggtgct ggtaaatcaa tggactttgt ttacaaatca gctaaacaac
    12601 gcttgtctcc acgcgatgta gaatttatcg ctgaatactc tggtcttcct gtgtatgtca
    12661 agggaccaca atgccgtgag gacgttgaac gttcacttgc tgcaggagct tctggtatct
    12721 gggtaaccaa ccacggtggt cgtcaaatcg acggtggacc agctgccttt gactcgcttc
    12781 aagaagtggc agaagcagtt gatagacgtg tgccgattgt ctttgactct ggtgttcgtc
    12841 gtggtcaaca cgtctttaaa gccttggctt caggagcaga cttggtagct attggtcgcc
    12901 ctgttattta tggcttggct ctcggtggta gtgtcggtgt gcgtcaagtc tttgaacact
    12961 tgaatgcgga attgaagaca gttatgcaat tgtctggagc tcagaccatt gaagatgtca
    13021 aacactttaa gctccgtcac aatccataca acccaacctt cccagttgac cctcgtgact
    13081 taaaattgta ttgataaaag agattgcctc cactgaatgt ggaggttttt cttcgtgtat
    13141 tggaagaata aattgctatc gaaaactata gtaaaatgaa ataagaatag gacaaatcaa
    13201 tcaggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattctagtt
    13261 tcaatatact atataaagat atttttcggt caaatatctt gctagggctt tcattttttg
    13321 ctatactggt tcagtaatta aataatagag acatttgggg tgctttaagc tgagatgata
    13381 cccattgaac ctgatacagt taagactggc gaagggaaat gtgaaacgtt ttttcgtatg
    13441 gcgaaatgaa cgatctaatc ttgtaagtca actctaattc ttgattgtaa ttggagtttt
    13501 ttgtttatat aaactggata ggcttgttta gttagctctc tgcaaatgct ctcgatgtgc
    13561 tttaaaagga tcaagtggaa ttacgatttt gcttgatatt tcttgctgtt ttattaatta
    13621 ctaacaagta aagagaggag aagtaaatat ggaaacacaa gactatgcat ttcagccagg
    13681 tttgactgtt ggagaattat taaaaagcag tcagaaggat tggcaggctg caatcaatca
    13741 tcgttttgtt aaggaacttt ttgcggggac aattgagaat aaggtcttaa aggactatct
    13801 gattcaagat tatcacttct ttgatgcctt cttatccatg ctgggtgcct gcgtagctca
    13861 cgcagataaa cttgaatcca agcttcgttt tgccaagcaa ctaggctttc ttgaagcaga
    13921 tgaagatggt tatttccaaa aggcttttaa agagttaaaa gtagcagaga atgactatct
    13981 agaagtgact ttgcaccctg taacaaaagc ctttcaggat ctaatgtatt cggcagtggc
    14041 ttcatcagac tatgcccatc ttttggttat gctggtcatt gcagaaggtc tctatttaga
    14101 ctggggttct aaagatttgg ctctacctga agtctatatt cattcggaat ggatcaatct
    14161 ccacagaggt cctttctttg cagagtgggt tcaatttctg gttgacgaac tcaatcgtgt
    14221 cggtaaaaat cgagaagatt tgacagaact tcagcaacgc tggaatcaag cagtcgcttt
    14281 agaattagct ttttttgata ttggttacga tgtctagaga aggctcatga tatttacaaa
    14341 tttgatgagg taagactctt cgaaaatctc ttcaaaccac gtcagttcta tccacaacct
    14401 caaaacagtg ttttgagctg acttcgtcag tcttatctac aggctcaaag cagtgttttg
    14461 agcaacctgc ggttagcctc ctagtttgct ctttgatttt cattgagtat aaatcattca
    14521 atgtaaatga aaaaaattct caactaaaga taaatcgaaa ttagtagatc gtataaagtg
    14581 aaaaggaagg atttcaatat gacaagttta aaattattaa aagaaaaagc acctttggtc
    14641 atttgcatca ccaatgatgt agtaaaaaat ttcacagcaa atggattagt agcactgggc
    14701 gcctcaccag ccatgagtga gtttccagta gatttagagg acttattaaa gtatgctggt
    14761 ggtttattaa taaacatagg aacattgaca gatgaaaatt ggaaattata ccaagctgct
    14821 ctgaaaattg cggagaaata taatgtccca gcagttttag atcctgtagc ctgtggagca
    14881 ggagaatata gaaaaaaagt agcagatgat ctaatcaaca attataaact agcagcgatt
    14941 agaggaaatg ctggcgagat tgcctcttta gtaggaatag atgtggcatc taaaggagta
    15001 gatagtgcgg gcgtagataa tattgacgaa attgctctag cagcaaatga gaagttcaat
    15061 attccaatag tagtaacagg tgaagtggat gccattgctg ttaatggaga agtggtaacg
    15121 attcataatg gtagtgctat gatgccgaaa gtcattggga caggatgctt attaggagct
    15181 gtagtagcaa gctttatcgg acttgaaaaa ggtcaagaat tgaaatcatt agaaacagca
    15241 atgttggttt acaatatcgc tggagaaatg gcggaaaaac gtccaaatgg acatcttcct
    15301 ggaacattta aagttgaatt tataaatgcc ttatatgaaa ttacagatga agatgtcaag
    15361 aaattcaaaa gagtgaagta agatgtttca taaagaatta ctaaaactat attttatttg
    15421 tggaacgact acttgccaag gaaaagatct atatacagtc gttgaggaag ccttaaaagg
    15481 tggtataacc ttatttcaat ttcgagaaaa aggtgagggt gctttagaag gcttagaaaa
    15541 acttgagcta gctatccaaa ttaaagaact ttgtaaaaaa tacaatgttc catttatcgt
    15601 taatgacgat atcgatttgg caatggaaat tgacgccgat ggcgtacatg taggtcaaga
    15661 tgatattggt gttgatgaaa ttagaaaatt gatgccagat aaaataattg gtctttctat
    15721 aaaaaacgag gaagaatttc aacaatcaaa agttgaatat gtagattatg ttggtgttgg
    15781 gcctgtattt gatacccagt caaaggatga tgctggtggt gctataggtt atgaaggtct
    15841 tgaattgatg agaaaactat tgccacaaat gcctttagtt gcaattggtg ggatacagac
    15901 gaaacatatt aaagacatta taaagaccaa tatggatggc gtttcaatca tttcggcaat
    15961 atcgtatgca aaaaatatag aaaaaactgt tcgggaaatg agtgaacaat agatatctat
    16021 ccttggattc taagtaagta ggattttcct tttttaaaaa actatgtcta gattcagctt
    16081 gaatggctaa ttcatcaaac aggtcattta ttttttgcaa aagaaagatc tgctgtcttt
    16141 gtgagtgcaa ggctgtattt ccagttcaat ttcttgaact agtataaaat gtgtgttata
    16201 ataatcacat aattaaataa taaaggcatt tggggtgctt taggctgaga tgatacccat
    16261 tgaacctgat acagttaaga ctggcgaagg gaaatgtgaa atgttttttt cgtatgtcga
    16321 aaagaactgt ctaatcttgt aagctgaact ctaattcgta tctgtaatta gagttttttc
    16381 ttttacgaaa ctggatgaag atgatatagt agattgaaat aagatgcgaa caaatcaatt
    16441 agaaaattca aattaatttc tagaaatatt ttagcattcc tgtgtactgt tctaaaatca
    16501 atctgctata caaagtattt aggtcggtct tcttgagtgc cccacttttc ttttaaaaaa
    16561 ggagtttttt atgttgaaaa aatggcagtt aaaagatgtt atcttgcttg ctttcttgtc
    16621 tatctttttt ggtggggttt tcgttggttc aggatatgtg tataatattc tcagtctact
    16681 cttaacacct cttggtttgc aggcctttgc caatgaaatc ctcttcggtc tctggtgtat
    16741 ggctgcgccc attgctgcca tctttgttcc gagagtcgga agtgcaacga ttggagaagt
    16801 gctagctgcg cttgctgaag tcctttatgg tagccaattt ggtctaggag ctcttttgtc
    16861 tggcttggtt caaggtttgg gaagtgaatt tggttttatc gtaactaaga atcgctatga
    16921 aagttggctc tctctaactg ctaatagtat tgggattacg cttgttagct ttgtctatga
    16981 atacattaag ttaggttact acgccttttc ccttccgttt gtcctttcct tacttgtggt
    17041 acgttttatt tctgtttatt tcttctgtac catcttggtt cgtgccattg tcaaactcta
    17101 tcatcagttt gcaactggag gaaaagcata gatgggtctg gaactacgag cgattcagtc
    17161 tccaatcttc cctgagccgt ttgattttac ttttcatgcg caagccttta ccttgttagt
    17221 tgggagcagt ggttcaggaa aatccagcct ttttcaagtc attgcccaag tcagttctcc
    17281 tccctatagt ggtcaagtcc tgatagatgg gagcgaggtc agtcagcttt ctatcatcgc
    17341 acgtgtccag aaggttggca ttctctttca aaatcccaat catcaattta ccatggagaa
    17401 cttgtttgag gagctgattt tcaccttgga gaatatcggc tatcaccttc aggaaattga
    17461 ttctaaaata gcagaggttg tccagcaatg tcgttgcgag gcaatcttac accgtcccat
    17521 tcatcaccta tcaggtgggg aaaagcaaaa agcagcgctg gctgtcctct ttgccatgaa
    17581 tcctagggta tatctcttgg atgagccctt cgcttccatt gaccgcaaga gcagaatcga
    17641 gatattggag attctaaaag agttggctct tgatgggaag acagttattt tgtgcgacca
    17701 tgatttatct gactataaag cctatatcga ccatatggtt gagctaagag acggaaaact
    17761 aagggaagtg tttcaaatcc cttcctatga gatgacagag gttgcttcaa aggaagttgc
    17821 ttctagcccg gaactattcc atatggatag aacgacttgt gagtttggaa atcgctccct
    17881 cttttcaatt gctgatttca cattctatca agggatttcc tgtatcctgg gtgacaatgg
    17941 tgtcgggaaa tcaaccttct ttcgctctat tcttcaattt caaaagtata aggggcgcat
    18001 tgcatggaag gggacagtcc tgaaaaagaa aaagagtttg taccgtgatc tgactggtgt
    18061 tgttcaggaa gctgagaagc agtttattcg agtcagtctg cgagaggagc ttcaattaga
    18121 tggacctgat tctgaaagaa atcagcggat ttttcaagct ttacgagatt ttgatttgga
    18181 gcaggcagtc gataagagtc cctatcaatt aagtggtggt cagcaaaaaa ttcttcagct
    18241 cttgaccatc ttgaccagta aggcttccgt gatcttgcta gatgaacctt ttgcaggttt
    18301 ggatgataga gcctgccatt atttttgcaa gtggattgtg gaggagagga atcaaggaag
    18361 aagttttctg ctcattagtc atcgtttaga ccctttgatt tctgtggttg attattggat
    18421 tgagatgact agtcagggtc ttcggcatgt gaaagaagtg accattacca aaccacttac
    18481 atctcagagt agcaataccc aaggggaggt gagatagtat ggtcaaagta gcaacccaga
    18541 caccgattat cagtctcttc ttgctgattt tatccttgga aacatctttc attccttcga
    18601 ttgctctgaa tctttcggta gtcgcatttt gtgttctctt tatgctctat taccgtcgat
    18661 ttaaaatgtt agcttggatg atcatacttg ccattttacc atcttttgcc aactactggg
    18721 cagttcagtt acacggagat gcttcacagg cagtcatgct tggaacgagg gcctttgtga
    18781 cagtttgtat cggccttgtc tttgtttcct ctatttcact aaaagagctt ctcttgtact
    18841 tggctcaaaa ggggctatca cgctcttggt cctatgcctt gattgtggta ttcaattctt
    18901 ttcctctcat tcagcaagaa atcaagtccc tcaaagaagc ttgcctatta cgtggtcaag
    18961 aactacattt ttggtcgccc ttgatttaca gtaaggttct gatgacagtc tttaggtggc
    19021 gccatcttta cctgagagct ctatctgctc acggatatga cgaacatgca cagttgaaga
    19081 atagctatcg gactttttat attcctaaaa aaacaaaatt aatctacctg cttttctttt
    19141 tattgcttca aaccagtcta tttttataaa ggagttatta tggaatttac agatattgcg
    19201 atggaattat ccaagaaagc ttggcaggtt tcctttcatc atccctttat tttacaatta
    19261 caagagggga atttagaacc tgccattttc cgctattacc tgattcagga tgcctactat
    19321 ctgaaggctt tctcagaaat ctatcatctt ttggctgata agacttcaaa ccaagagatg
    19381 aaaagacttt tgaaacaaaa tgctcagggt ttagtggagg gtgagttatt tattcgccaa
    19441 caatttttca aggaattgga aatcaacgac caggaaatgg agcaacatcc aatcgctcca
    19501 acctgttatc attacatttc tcatatttat aggcaatttg cagaaccgaa cttagccata
    19561 gcttttgcaa gcttgcttcc ttgtccttgg ttataccatg atataggcaa atcacttaat
    19621 cttaaaccat caccaaatcc tctctatcaa caatggattg aaacttatat tacggatgag
    19681 ttagagcaac agatcagaga ggagggagca ctggtcaatc agctctatcg agaaagtgac
    19741 gagacagaca agcaaaaaat gctagatgcc ttccacatca gtgttcatat ggaagccaag
    19801 ttttgggaaa tggcctacca acaccagaca tggaagagcg atttacagtc tttagaaaaa
    19861 ggagaagaat agaaacatga gaaagcacca attacaagtt cacaaattaa ccattttatc
    19921 tatgatgatt gcccttgatg tagtccttac acctatcttt cgaattgagg gaatggcacc
    19981 gatgtccagt gtagtcaata ttctagcggg aatcatgatg ggacctgttt atgccttggc
    20041 tatggctaca gtgacagcca ttatccgtat gacgactcaa gggattccgc ctttagctct
    20101 cacaggagcg acttttggag cccttctagc aggtctcttt tataagtacg gtcgaaaatt
    20161 ttacttttct gccttgggag aaattgtggg aacaggtatt attggttcta ttgtttccta
    20221 tcctgttatg gtactcttta caggatcagc tgctaagctt agctggttta tctacacgcc
    20281 tcgatttttc ggagcaacct tgattggtac agcgatttcc tttattgcct ttcgattttt
    20341 aatcaagcag gaattcttta aaaaagtgca gggatatttc tttagtgaaa ggatagactg
    20401 atgcaggaat ttacaaatcc ctttcctata ggctctagtt ccctcattca ctgtatgacc
    20461 aatgagattt cttgtgagat gctggcaaat gggattttgg ctctgggatg caaacctgtc
    20521 atggcagatg atccccgtga ggttcttgat tttactaagc aaagtcaggc tctcttcatc
    20581 aatttggggc atttgtcagc tgagaaggag aaagcaatcc gtatagcagc ttcttatgta
    20641 gctcaagttt gtctcccaat ggtagtagat gcggttggcg taacgacttc atccattcgt
    20701 aagagcttag ttaaagacct tttagactat agacctacgg tccttaaagg aaacatgtca
    20761 gaaattcgaa gtcttgttgg attaaagcac cacggcgttg gggtcgatgc gagtgctaaa
    20821 gatcaagaaa cggaggattt gcttcaagtc ttgaaagatt ggtgtcagac ctatcctggt
    20881 atgtcattct tagttactgg ccccaaggac ctcatcgttt cgaaaaatca ggtcgctgta
    20941 ctggagaatg gctgtactga attagactgg ataacaggga caggagattt ggttggagcc
    21001 ttaacagccg tttttctcag ccaaggaaag actggttttg aagcttcttg cttagcagtc
    21061 tcttatctca atatcgctgc tgagaaaata gttgttcaag gaatgggatt ggaagaattt
    21121 cgttaccaag tactcaatca gctttcgctc ctaagaagag atgaaaattg gctagatacc
    21181 atcaaaggag aggtttatga atagagaagc acttagacta tatctggtaa ccaatcgcta
    21241 tcaagattcc gtggaaagct ttcttgcaaa agttgagacg gcctgccgtt caggggttac
    21301 catagtccaa ttgcgagaaa aaaatctcac aaccaatcaa tattatcaac tggcaaaaca
    21361 agtcaaggaa ataacagttg cttatcaggt acccttgata atcgatgatc ggttggatgt
    21421 ttgtcttgcg gttgatgcag caggtctgca tatcggagat gatgaattac cagtttcggt
    21481 agctcgcaaa gtcttgggtc ctgaaaaaat cctcggtgtc accgctaaaa cggttaaaag
    21541 agctctggaa gcggagaaat caggtgcaga ttacttgggg acaggagcca tttttccgac
    21601 aaccaccaag gaaaatgcac ccatcaccct gatttcaacc ttgaaaacaa tttgtcaaac
    21661 ggttgccatt ccagtagttg ctattggcgg cttgacatca gagaatattg accaacttat
    21721 cggcacgggt atagctggtg tagctgttgt acgtgatttg atgcaagcag aagatattga
    21781 ggcaaaaacg caagcctttt taacaaagtt gcatgacatt ctttcctaat taatactcaa
    21841 tgaaaatcaa agagtaaact agaaagctag tcgcaggttg ctcaaagtac tgccttgagg
    21901 ttttagataa gactgacgaa gtcagctcaa aacagtgttt tgaagttgca gatggaagct
    21961 gacgtagttt gaagagattt tcgaagagta taaaaactct ctaattccct taaagtggga
    22021 actagagagt tttttttctt aatctttaaa agttgtatgg ttgactggac cagaaccatg
    22081 accgagttga ggggcatctt gaatagcttt tgtgataaag gccttggcct tatcaactgc
    22141 ctggtaaaga ctcttgccct tggctagttc agcagtaatc actgcagcaa aggtacatcc
    22201 agtaccatgg gtgtgacagg tttgaattcg tgggctttcc cagacaaatt gttcattctt
    22261 ggtaaagagg aaatctttag caccaccttt gagatgtccg cctttgataa ccacagactg
    22321 aggaccaaat tcttttaaaa tcaggcgacc agcacgctgc atgtcttcgg ggtcatggat
    22381 tgaaaaacca acaatctctt ctgcttcagg aagatttggc gtaataatag ttgctagagg
    22441 tagtaagttt gttttgagat agtctctagc acttgagtca atcaaggcat ctccacttgt
    22501 agcaaccata acaggatcaa ggacataggg acaatccagt tttttaagat agggttggat
    22561 gatttccatg atttcagtag tagccaacat tccagttttt acagcctgag gtggaatatc
    22621 agaaaagaca ctctccaatt gggctttcaa catttgagga gaaacgtgct cgattagctg
    22681 aacacctctg gtattttgag cgacaagact ggttacaaca gccattccat agacatctct
    22741 cgcttggaat gactttaaat ctgccataat gccagcacca ccactagggt cagtccctgc
    22801 aatggtcaaa gcaacgggta aataagtcat ctaaaacctc ccaaccaact gaagtttgta
    22861 ttcttaagaa caagctagtc tgttttagaa aggcaataga agactgctag tccccattat
    22921 catttccact tccatcagct agcattacct agattgagtc agagggatct aacagtcgtt
    22981 aatctcagct ttacgcaccc ctagtggttg ttttcttttt tctttagtat agcatatttt
    23041 tattgtttat atagtaatat ttgactgcaa atcgtttttc aaatgattaa aattctattt
    23101 ttcgaagaaa atattatttc tataattgac aaatgtagat tttaagagta tactgatgag
    23161 tgtaattgac aaatgtagat tttggaggta tggtaatgca gatttcagat gcagaatggc
    23221 aggtcatgaa gattatttgg atgcaggggg agcagaccag tacggatttg attagggttt
    23281 tggcagagcg gtttgactgg tccaagtcca cggttcagac acttttggct cgtctagttg
    23341 agaaagagtg tttgactcgg aaaaaagaag gcaagtcctt tgtttattca gcccttttaa
    23401 ctctagacca aagtcgggat ttacttgtcc aagatatcaa agacaaggtt tgttcccgta
    23461 ggattaggaa tttgttggct gacttgattg tagaatgtga atttactcag actgacttgg
    23521 aagacttgga agctgtgatt tcagagaaga aatcaagtgc tgtaacagaa gtaagatgta
    23581 attgtatgta aaggaaacgt catgttaaat agtattgtaa ccattatttg tattgccctt
    23641 atcgcgttta tcttgttttg gtttttcaaa aagcctgaaa aatctggaca aaaagcccag
    23701 caaaaaaacg gataccaaga gattcgagtg gaagtcatgg gaggctatac tcctgagttg
    23761 attgtcctca agaaatcagt gccagcacgc attgtctttg atcgcaagga tccttcacca
    23821 tgtctggatc aaattgtttt tccagatttt ggtgtacatg cgaacctgcc aatgggggaa
    23881 gagtatgtag tggaaatcac gcctgagcag gctggagagt ttggctttgc ttgtggtatg
    23941 aacatgatgc acggcaagat gattgtagag taggtggaga ctatgacaga aattgtgaaa
    24001 gcaagcttag aaaatggcat tcaaaaaatc cgtatccgag ctgaaaaagg ctatcatcca
    24061 acccatatcc agcttcaaaa gggaattcca gctgagatta cctttcatcg tgctactcct
    24121 tcaaactgtt ataaggaaat tctgtttgaa gaagaaggta tcttggaacc aatcggcgta
    24181 gatgaggaga aagtcattcg ttttacacct caagaattag ggcgacatga attttcttgt
    24241 ggcatgaaga tgcaaaaggg aagctataca gtcgttgaga agactcgaaa atctctatct
    24301 ctcctgcaac gtttttggat tattagtatc tttactgtgc ctcttgtgat tctcatgatt
    24361 gggatgttga caggtagcat tagtcatcaa gtcatgcatt ggggaacctt tctagcaaca
    24421 acgcctatta tgttagttgc aggtaagcca tatatccaga gtgcttgggc cagttttaaa
    24481 aagcacaatg ccaacatgga taccttggtt gcgctgggaa ctctagtggc ttatttctat
    24541 agcctagttg ctctctttgc tggtctccct gtttacttcg aaagtgctgg atttatcctc
    24601 tttttcgttc ttttgggagc agtttttgag gaaaaaatga gaaaaaatac gtcccaagct
    24661 gtggagaaat tactggactt gcaagctaaa accgcagaag tcttgagtga tgatagttat
    24721 gtccaagttc ctttggaaca agtcaaggta ggcgacctga ttcgagtgcg tcccggtgaa
    24781 aagattgctg ttgatggtgt cgtagtagaa ggtgtctcta gtattgacga atccatggtg
    24841 acaggtgaga gtctgcctgt ggacaagaca gttggagata ctgtcattgg ctcaaccatc
    24901 aatcatagtg gaacgcttgt ctttagagca gaaaaagttg gctcagagac tgttttggct
    24961 cagattgtgg attttgtgaa gaaagctcag acaagtcgtg cgccgattca ggacttgacg
    25021 gataagattt cagggatttt tgtcccagta gttgtcattt taggaatcat gaccttttgg
    25081 gtttggttcg tcttgctcag ggatagtgtg gttgtgcttg gagctagctt tgtgtcctct
    25141 cttctctacg gagtggcggt tttgattatc gcctgtcctt gtgccttggg acttgcaaca
    25201 ccgacagccc ttatggtggg gacaggacgt agtgccaaga tgggggttct cctcaaaaat
    25261 ggaactgtct tacaggaaat ccagaaagtt caaactcttg tctttgataa gaccgggact
    25321 ttgacggaag ggaaacctgt ggtaacagat atcatcggcg acgaagtaga agtgtttgga
    25381 ttggcagcct ccttggaaga tgcttctcaa cacccactgg ctgaggctat cgttaagcga
    25441 gcgagtgaag ctggacttga gtttcaaact gttgaaaatt ttcaggcctt gcacgggaaa
    25501 ggtgtttcag ggcgaatcaa tggaaaacaa gttttacttg gaaatgctaa aatgctggat
    25561 ggcatggata tttctaatac ttatcaagat aaactagaag aactagaaaa agaagctaag
    25621 acagttgtgt ttttagctgt tgacaatgaa atcaaaggct tgcttgcttt gcaagatatt
    25681 cctaaggaaa atgctaagct agccatcagt cagctaaaaa aacgtggtct ccgaacagtc
    25741 atgctgacag gagacaatgc tggtgtggcg cgtgctattg cagatcaaat cggaattgaa
    25801 gaggtcattg caggcgtctt gccagaagaa aaagcccatg aaatccataa actgcaacag
    25861 tcaggtaaag tagcctttgt tggggacggt atcaatgacg ctcctgccct tagtgtagca
    25921 gatgtgggaa ttgctatggg tgctggaaca gatatcgcca tcgagtcagc agatttggtg
    25981 ttgacaacca ataatctttt aggagtggtt cgtgcctttg atatgagtaa gaaaaccttt
    26041 catcgaattc tactcaatct tttctgggct tttatctaca atgttgtcgg aattccgatt
    26101 gcagcaggag tcttttcagg tgttggactg gctctcaacc cagaattggc aggtctagcc
    26161 atggccttta gttctgtatc tgttctgacc agttcccttc tgttaaattt tagtaaaata
    26221 gactaaaagt aatgactaga tttctttgtt ataaaacaga aacgacaaca ctttcaaaac
    26281 tgtattagaa tagagaatag agagttttga gcagattttt agaaaagtca gcataaatat
    26341 gatacagtgg aatagtaaaa atttggagaa cgtttccaat tctatgtaat cgtattctcc
    26401 aagtttaaaa aaattgaagg agagttatca ttatgactca agggaaaatt actgcatctg
    26461 cagcaatgct taacgtattg aaaacatggg gcgtagatac aatctacggt atcccatcag
    26521 gaacactcag ctcattgatg gacgctttgg ctgaagacaa agatatccgc ttcttacaag
    26581 ttcgccacga agagacaggt gctcttgcag cggttatgca agctaaattc ggcggctcaa
    26641 tcggggttgc agttggttca ggtggtccag gtgcgactca cttgattaac ggtgtttacg
    26701 atgcagctat ggataacact ccattcctag cgatccttgg atcacgtcca gttaacgaat
    26761 tgaacatgga tgctttccaa gagcttaacc aaaacccaat gtacaacggt atcgctgttt
    26821 acaacaaacg tgtagcttac gctgagcaat tgccaaaagt aattgacgaa gcctgccgtg
    26881 ctgcaatttc taaaaaaggt ccagctgttg ttgaaattcc agtaaacttc ggtttccaag
    26941 aaatcgatga aaactcatac tacggttcag gttcatacga acgctcattc atcgctcctg
    27001 ctttgaacga agttgaaatc gacaaagctg ttgaaatctt gaacaatgct gaacgcccag
    27061 ttatctatgc tggatttggt ggtgttaaag ctggtgaagt gattactgaa ttgtcacgta
    27121 aaatcaaagc accaatcatc acaactggta aaaactttga agctttcgaa tggaactatg
    27181 aaggtttgac aggttctgct taccgtgttg gttggaaacc agccaacgaa gtggtctttg
    27241 aagcagacac agttcttttc cttggttcaa acttcccatt tgctgaagtt tacgaagcat
    27301 tcaagaacac tgaaaaattc atccaagtcg atatcgaccc ttacaaactt ggtaaacgtc
    27361 atgcccttga cgcttcaatc cttggtgatg ctggtcaagc agctaaagct atccttgaca
    27421 aagtaaaccc agttgaatca actccatggt ggcgtgcaaa cgttaagaac aaccaaaact
    27481 ggcgtgatta catgaacaaa ctcgaaggta aaactgaggg tgaattgcaa ttgtatcaag
    27541 tttacaatgc aatcaacaaa catgctgatc aagacgctat ctactcaatc gacgtaggta
    27601 acactactca aacatctact cgtcaccttc acatgacacc taagaacatg tggcgtacat
    27661 ctccactctt tgcgacaatg ggtattgccc ttcctggtgg tatcgctgct aagaaagaca
    27721 atccagatcg ccaagtatgg aacatcatgg gtgacggagc attcaacatg tgctacccag
    27781 acgttatcac aaacgttcaa tacgaccttc cagttatcaa ccttgtcttc tcaaatgctg
    27841 agtacggctt catcaagaac aaatacgaag atacaaacaa acacttgttt ggtgtagact
    27901 tcacaaacgc tgactacgct aaaattgcgg aagctcaagg agctgttgga ttcacagttg
    27961 accgtatcga agacatcgat gcagttgttg cagaagctgt taaattgaac aaagaaggta
    28021 aaactgttgt catcgatgct cgcatcactc aacaccgtcc acttccagta gaagtacttg
    28081 aattggatcc aaaacttcac tcagaagaag ctatcaaagc cttcaaggaa aaatacgaag
    28141 cagaagaact cgtaccattc cgtctcttct tggaagaaga aggattgcaa tcacgcgcaa
    28201 ttaaataatt cctctcgccg aaaatcaaat atgaaacttg tagactttat tctttgattt
    28261 tcatagagca gaactttaaa agatcggagc aatccggtct ttttatggag gagagtaaat
    28321 gaatttaaat caattagata ttatcgtttc aaatgttccc caagtctgtg ctgacttgga
    28381 gcatattttg gataaaaagg cagattatgc tgatgatggt tttgctcagt ttacgattgg
    28441 tagtcactgc cttatgttgt cacaaaatca tttggttcct ttggaaaact ttcagtcagg
    28501 aatcattatt catatcgagg ttgaggatgt agaccagaac tacaaacggt tgaacgagct
    28561 tggtataaag gttttacacg gaccaactgt aaccgattgg ggaacagagt ccttattagt
    28621 tcaaggacct gctggtctag tgcttgattt ttatcgtatg aaataggtcg cgtagtcatt
    28681 tgacgttaac cttagctatt tttaaaatag aaattgagaa tatgaactat tgaaagatcg
    28741 gagcaatccg gtctttttat ataaagaaat tgcatataat actcatctgc tttcaaaaag
    28801 cattctagtc catctccgat taacgatgga ctttatcacc tccttctcca gtccttgtat
    28861 gacatcttgg agttgattca tgacatcttc caaagttcga aaggctttat tcttaaatcc
    28921 acgtttacga atctctttcc acacttgttc aatggggttc atctctggtg tgtatggagg
    28981 aataaatgca aagccaatat tagtcggaat ctttaaggta cttgatttat gccatatagc
    29041 attgtctata acgagtaaaa aataatcata tggataagct tgtgaaagct cttctaaaaa
    29101 ggcgttcatc cactcagtat tacatctacc agctattaag aaaaatgatt cgcctgtatg
    29161 ggcatcaaca gctccataac aatagcgaaa ttctcgtata tagtgactat ggacatgtgg
    29221 acctactcct attggagacc aacaagatcc cagtttactg attctaccga aaccagcttc
    29281 atcttggtac atcaagcgaa ccttatgaaa aggtctactg gttttaaaac gctttcttgt
    29341 cttcttgaat tgagatttta tttttagacg cgacaatggt ttgagcgtct gctttcttag
    29401 gatgttctgg acgtggcgta atatttcgcc aaccatggcg cttcaacagt tgatagaagg
    29461 catcacgtgt gtaggaacga cctaactcct ttttataagc ctgaaataag gcatcaattg
    29521 taacaaattc tcctgcctct gta
//
LOCUS       DAVLRT010000018        28520 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLRT010000018 DAVLRT010000000
VERSION     DAVLRT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.018"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            143..2125
                     /locus_tag="VXM85_000798"
     CDS             143..2125
                     /locus_tag="VXM85_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1345416.1"
                     /translation="MKSSRLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSEEQKTSTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVVIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2426..7729
                     /locus_tag="VXM85_000799"
     CDS             2426..7729
                     /locus_tag="VXM85_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1345417.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPRAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRSDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(7896..10055)
                     /locus_tag="VXM85_000800"
     CDS             complement(7896..10055)
                     /locus_tag="VXM85_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1345418.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10052..10648)
                     /gene="recU"
                     /locus_tag="VXM85_000801"
     CDS             complement(10052..10648)
                     /gene="recU"
                     /locus_tag="VXM85_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1345419.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10714..11241
                     /locus_tag="VXM85_000802"
     CDS             10714..11241
                     /locus_tag="VXM85_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1345420.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGTLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11311..11640
                     /gene="gpsB"
                     /locus_tag="VXM85_000803"
     CDS             11311..11640
                     /gene="gpsB"
                     /locus_tag="VXM85_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1345421.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11661..12041
                     /gene="rnpB"
                     /locus_tag="VXM85_000804"
     ncRNA           11661..12041
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VXM85_000804"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12126..13283
                     /locus_tag="VXM85_000805"
     CDS             12126..13283
                     /locus_tag="VXM85_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1345422.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLCSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13296..14690
                     /gene="mapZ"
                     /locus_tag="VXM85_000806"
     CDS             13296..14690
                     /gene="mapZ"
                     /locus_tag="VXM85_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1345423.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14766..16190
                     /gene="gndA"
                     /locus_tag="VXM85_000807"
     CDS             14766..16190
                     /gene="gndA"
                     /locus_tag="VXM85_000807"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1345424.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSHKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVYASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16202..16891
                     /locus_tag="VXM85_000808"
     CDS             16202..16891
                     /locus_tag="VXM85_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1345425.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVAYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESVTETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            16989..17945
                     /gene="cbpC"
                     /locus_tag="VXM85_000809"
     CDS             16989..17945
                     /gene="cbpC"
                     /locus_tag="VXM85_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1345426.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGQFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEPKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYFGNKWYYLNAGN
                     GDMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYNGEWVQ"
     gene            18067..18945
                     /gene="mvk"
                     /locus_tag="VXM85_000810"
     CDS             18067..18945
                     /gene="mvk"
                     /locus_tag="VXM85_000810"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1345427.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFWVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            18927..19880
                     /gene="mvaD"
                     /locus_tag="VXM85_000811"
     CDS             18927..19880
                     /gene="mvaD"
                     /locus_tag="VXM85_000811"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1345428.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            19867..20874
                     /locus_tag="VXM85_000812"
     CDS             19867..20874
                     /locus_tag="VXM85_000812"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1345429.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            20858..21868
                     /locus_tag="VXM85_000813"
     CDS             20858..21868
                     /locus_tag="VXM85_000813"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1345430.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDNS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            21945..22643
                     /locus_tag="VXM85_000814"
     CDS             21945..22643
                     /locus_tag="VXM85_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1345431.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            22640..23635
                     /locus_tag="VXM85_000815"
     CDS             22640..23635
                     /locus_tag="VXM85_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1345432.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FFFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            23649..24281
                     /locus_tag="VXM85_000816"
     CDS             23649..24281
                     /locus_tag="VXM85_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1345433.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            24282..24523
                     /locus_tag="VXM85_000817"
                     /pseudo
     CDS             24282..24523
                     /locus_tag="VXM85_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            24505..24759
                     /locus_tag="VXM85_000818"
     CDS             24505..24759
                     /locus_tag="VXM85_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1345434.1"
                     /translation="MAANYLKAMQSYLKETDLPKLEQLVVTKSWWDTVDILDRVVGSL
                     VYEHSELEEIILILFENLFKPRQLYLQPQNSVLSNLRLAS"
     gene            24784..25050
                     /locus_tag="VXM85_000819"
                     /pseudo
     CDS             24784..25050
                     /locus_tag="VXM85_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            25200..25439
                     /locus_tag="VXM85_000820"
     CDS             25200..25439
                     /locus_tag="VXM85_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345435.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            25520..26341
                     /gene="cbpG"
                     /locus_tag="VXM85_000821"
     CDS             25520..26341
                     /gene="cbpG"
                     /locus_tag="VXM85_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV1345436.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            26360..27382
                     /gene="cbpF"
                     /locus_tag="VXM85_000822"
     CDS             26360..27382
                     /gene="cbpF"
                     /locus_tag="VXM85_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEV1345437.1"
                     /translation="MKLLKKMMQIALATFFFGLLATNTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLNPATGI
                     MQTGWQYLGNRWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQESSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAINTTVGGYYLNY
                     NGEWVK"
     gene            complement(27510..28520)
                     /locus_tag="VXM85_000823"
                     /pseudo
     CDS             complement(27510..28520)
                     /locus_tag="VXM85_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8689 a         5136 c         6519 g         8176 t
ORIGIN      
        1 tatagttgag ggatttttaa atgatattca tattttttgc aaagatgttg tttgaaaaat
       61 aattttcaaa aattctgaaa attctgttga caactttctg aaaagagtct ataatggaga
      121 gaaagtttta aaggagaaaa tgatgaaaag ttcaagacta tttgcccttg cgggcgtgac
      181 attattggcg gcgactactt tagctgcatg ctctggatca ggttcaagca ctaaaggtga
      241 gaagacattc tcatacattt atgagacaga ccctgataac ctcaactatt tgacaactgc
      301 taaggctgcg acagcaaata ttaccagtaa cgtggttgat ggtttgctag aaaatgatcg
      361 ctacgggaac tttgtgccgt ctatggctga ggattggtct gtatccaagg atggattgac
      421 ttacacttat actatccgta aggatgcaaa atggtatact tctgaaggtg aagaatacgc
      481 ggcagtcaaa gctcaagact ttgtaacagg actaaaatat gctgctgata aaaaatcaga
      541 tgctctttac cttgttcaag aatcaatcaa agggttggat gcctatgtaa aaggggaaat
      601 caaagatttc tcacaagtag gaattaaggc tctggatgaa cagacagttc agtacacttt
      661 gaacaaacca gaaagcttct ggaattctaa gacaaccatg ggtgtgcttg cgccagttaa
      721 tgaagagttt ttgaattcaa aaggggatga ttttgccaaa gctacggatc caagtagtct
      781 cttgtataac ggtccttatt tgttgaaatc cattgtgacc aaatcttctg ttgaatttgc
      841 gaaaaatccg aactactggg ataaggacaa tgtgcatatt gacaaagtta aattgtcatt
      901 ctgggatggt caagatacca gcaaacctgc agaaaacttt aaagatggta gccttacagc
      961 agctcgtctc tatccaacaa gtgcaagttt cgcagagctt gagaagagta tgaaggacaa
     1021 tattgtctat actcaacaag actctattac gtatctagtt ggtacaaata ttgaccgtca
     1081 gtcctataaa tatacatcta agaccagtga agaacaaaaa acatctacga aaaaggctct
     1141 cttaaacaag gatttccgtc aggctattgc atttggattt gaccgtacag cctatgcctc
     1201 tcagttgaat ggacaaactg gagcaagcaa aatcttacgt aatatctttg ttccaccaac
     1261 atttgttcaa gcagatggta aaaactttgg cgatatggtc aaagagaaat tggtcactta
     1321 tggggatgaa tggaaggatg ttaatcttgc agattctcag gatggtcttt acaatccaga
     1381 aaaagccaag gctgaatttg ctaaagctaa atcagcttta caagcagaag gtgtgacttt
     1441 cccaattcat ttggatatgc cagttgacca gacagcaact acaaaagttc agcgcgtcca
     1501 atctatgaaa caatccttgg aagcaacttt aggagcggat aatgtagtca ttgatattca
     1561 acaactacaa aaagacgaag taaacaatat tacatatttt gctgaaaatg ctgctggtga
     1621 agactgggat ttatcagata atgtcggttg gggtccagac tttgccgatc catcaaccta
     1681 ccttgatatc atcaaaccat ctgtaggaga aagtactaaa acatatttag ggtttgactc
     1741 aggggaagat aatgtagctg ctaaaaaagt aggtctatat gactacgaaa aattggttac
     1801 tgaggctggt gatgagacta cagatgttgc taaacgctat gataaatacg ctgcagccca
     1861 agcttggttg acagatagtg ctttgattat tccaactaca tctcgtacag ggcgtccaat
     1921 cttgtctaag atggtaccat ttacaatacc atttgcattg tcaggaaata aaggcacaag
     1981 tgaaccagtc ttgtataaat acttggaact tcaagacaag gcagtcactg tagatgaata
     2041 ccaaaaagct caggaaaaat ggatgaaaga aaaagaagag tctaataaaa aggctcaaga
     2101 agatctcgca aaacatgtga aataactgtt gcaaaatata agaaaggatt tagtatttcc
     2161 cttgaatgct gaatcctttt ttacatttgt aaagaaagat tctaaatgta ctgaccccca
     2221 aaagttggag cctctttttg tcagaataga gaaaattttt gttaatttta cttgtttcct
     2281 attgctttct cagctattat ttgttatatt aaaagcataa ttatttttta tttatcaggg
     2341 ttaagcattg cactttcaga ggaaggagta ttttttaaaa aggaaatgta aacgcttgct
     2401 aaaaaatgaa aggatttaga agtttatgaa taaaggatta tttgaaaaac gttgtaaata
     2461 tagtattcgg aaattttcat taggtgttgc ttctgttatg attggagctg cattctttgg
     2521 gacaagtccg gttcttgcag atagcgtgca gtctggttcc acggcgaact taccagctga
     2581 tttagctact gctcttgcaa cagcaaaaga gaatgatgga cgtgattttg aagcgcctaa
     2641 ggtgggagaa gaccaaggtt ctccagaagt tacagatgga cctaagacag aagaagaact
     2701 attagcactt gaaaaagaaa aaccggctga agaaaaacca aaagaggata aacctgcagc
     2761 tgctaaacct gaaacaccta agacggtaac ccctgaatgg caaacggtag agaaaaaaga
     2821 acaaaaggga acagtcacta tccgagaaga aaaaggtgtc cgctacaacc aactatcctc
     2881 aactgctcaa aatgataacg caggaaaacc agccctgttt gaaaagaagg gcttgaccgt
     2941 tgatgccaat ggaaatgcaa ctgttgattt aaccttcaaa gatgattctg aaaagggcaa
     3001 atcacgcttt ggtgtcttcc tgaaatttaa agataccaat aataatgttt ttgtcggtta
     3061 tgacaaggat ggctggttct gggagtataa atctccaaca actagcactt ggtatagagg
     3121 tagtcgtgtt gctgctcctg aaacaggatc aacaaaccgt ctctctatca ctctcaagtc
     3181 agacggtcag ctaaatgcca gcaataacga tgtcaatctc tttgacacag tgactctacc
     3241 agctgcggtc aatgaccatc ttaaaaatga gaagaagatc cttctcaagg cgggctctta
     3301 tggcaatgac cgtacggttg tcagcgttaa aacagacaat caagaaggcg taaaagcgga
     3361 tgatactcct gcccagaaag aaacaggtcc ggttgttgac gatagcaagg tgacttatga
     3421 tacgatccag tctaaggtac ttaaggcagt aattgaccaa gccttccctc gtgtcaagga
     3481 atacagcttg aacgggcata ctttgccagg acaggtgcaa cagttcaacc aagtctttat
     3541 caataaccac cgaatcaccc ctgaagtcac ttataagaaa atcaatgaga caacagcaga
     3601 gtacttgatg aagcttcgcg atgatgctca cttaatcaat gcggaaatga cagtacgcct
     3661 gcaagttgtg gacaatcaat tgcactttga tgtgaccaag attgtcaacc acaatcaagt
     3721 cactccaggt caaaagattg atgacgaaag aaaactactt tcttctatta gtttcctcgg
     3781 caatgcttta gtctctgttt ctagtgatca aactggtgct aagtttgatg gggcaaccat
     3841 gtcaaacaat acgcatgtca gcggagatga tcatatcgat gtaaccaatc caatgaaaga
     3901 cctagccaag ggttacatgt atggatttgt ttctacagat aagcttgctg ctggtgtttg
     3961 gagtaactct caaaacagct atggtggtgg ttcgaatgac tggactcgtt tgacagctta
     4021 taaagaaaca gtcggaaatg ccaactatgt aggaatccac agctctgaat ggcaatggga
     4081 aaaagcttat aagggcattg ttttcccaga atacacgaag gaacttccaa gggctaaggt
     4141 tgttatcact gaagatgcca atgcagacaa gaaagtcgat tggcaggatg gtgccattgc
     4201 ttatcgtagc attatgaaca accctcaagg ttgggaaaaa gttaaggata tcacagctta
     4261 ccgtatcgcg atgaactttg gttctcaagc acaaaaccca ttccttatga ccttggatgg
     4321 tatcaagaaa atcaatctcc acacagatgg tcttgggcaa ggtgttctcc ttaaaggata
     4381 tggtagcgaa ggccatgact ctggtcactt gaactatgct gatattggta agcgtatcgg
     4441 tggtgtcgaa gacttcaaga ccctgattga gaaggctaag aaatatggag ctcatctagg
     4501 tatccacgtt aacgcttcag aaacttatcc tgagtctaaa tacttcaatg aaaaaattct
     4561 ccgtaagaat ccagatggaa gctatagcta tggttggaac tggctagatc aaggtatcaa
     4621 cattgatgct gcctatgacc tagctcatgg tcgtttggca cgttgggaag atttgaagaa
     4681 aaaacttggt gacggtctcg actttatcta tgtggacgtt tggggtaatg gtcaatcagg
     4741 tgataacggt gcctgggcta cccacgttct tgctaaagaa attaacaaac aaggctggcg
     4801 ctttgcgatc gagtggggcc atggtggtga gtacgactct accttccatc actgggcagc
     4861 tgacttgacc tacggtggct acaccaataa aggtatcaac agtgccatca cccgctttat
     4921 ccgtaaccac caaaaagatg cttgggtagg ggactacaga agttatggtg gtgcagccaa
     4981 ctatccactg ctaggtggct acagcatgaa agactttgaa ggctggcaag gaagaagtga
     5041 ctacaatggc tatgtgacta acctctttgc tcatgacgtc atgactaagt acttccaaca
     5101 cttcactgta agtaaatggg aaaatggtac accggtgact atgaccgata acggtagcac
     5161 ctataaatgg actccagaaa tgcgagtgga gttggtagat gctgacaata ataaagtagt
     5221 tgtaactcgt aagtcaaatg atgtcaatag cccacaatat cgcgaacgta cagtaacgct
     5281 caacggacgt gtcatccaag atggttcagc ttacttgact ccttggaact gggatgcaaa
     5341 tggtaagaaa ctttctactg agaaggaaaa gatgtactac ttcaatacgc aggccggtac
     5401 aacaacttgg acccttccaa gcgattgggc aaagagcaag gtttaccttt acaagctaac
     5461 tgaccaaggt aagacagaag agcaagaact aactgtaaaa gatggtaaaa ttaccctaga
     5521 tcttctagca aatcaaccat acgtacttta ccgttcaaaa caaaccaatc ctgaaatgtc
     5581 atggagtgaa ggcatgcaca tctatgacca aggatttaat agtggaacct tgaaacactg
     5641 gaccatttca ggcgatgctt ctaaggcaga aattgtcaag tctcaagggg caaacgatat
     5701 gcttcgtatt caaggaaaca aagaaaaagt tagtctcact cagaaattaa ctggcttgaa
     5761 accaaatacc aagtatgccg tttatgtcgg tgtcgataac cgtagtaatg ccaaggcaag
     5821 tatcactgtg aatactggtg aaaaagaagt gactacttat accaataagt ctctcgccct
     5881 caactatgta aaagcctacg cccacaatac acgtcgtaac aatgctacag ttgacgatac
     5941 aagttacttc caaaacatgt acgccttctt tacaactgga tcggacgtct caaatgttac
     6001 tctgacattg agtcgtgaag ctggtgatca agcaacttac tttgatgaaa ttcgtacctt
     6061 tgaaaacaat tcaagcatgt acggagacaa gcatgataca ggtaaaggca ccttcaagca
     6121 agactttgaa aatgttgctc agggtatctt cccatttgta gtgggtggtg ttgaaggtgt
     6181 tgaagataac cgcactcact tgtctgaaaa acacgatcca tatacacaac gtggttggaa
     6241 tggtaagaaa gtcgatgatg ttatcgaagg aaattggtca ctcaagacaa atggactagt
     6301 gagccgtcgt aacttggttt accaaactat tccgcaaaac ttccgttttg aagcaggtaa
     6361 gacctaccgt gtaacctttg aatacgaagc aggttcagac aatacctatg cttttgtagt
     6421 cggtaaggga gaattccagt caggtcgtcg tggtactcaa gcaagcaact tggaaatgca
     6481 tgaattgcca aatacttgga cagattctaa gaaagccaag aaggtaacct tccttgtgac
     6541 aggtgcagaa acaggggata cttgggtagg tatctactca actggcaatg caagtaatac
     6601 tcgtagtgat tctggtggaa atgccaactt ccgtggttat aacgacttca tgatggataa
     6661 tcttcaaatc gaagaaatta ccctaacagg taagatgttg acagaaaatg ctctgaagaa
     6721 ctacttgcca acggttgcca tgactaacta caccaaagag tctatggatg ctttgaaaga
     6781 ggcggtcttt aacctcagtc aggccgatga tgatatcagt gtggaagaag cgcgtgcaga
     6841 gattgccaag attgaagcct tgaagaatgc tttggttcag aagaaaacgg ctttggtagc
     6901 agaagacttt gcaagtctta cagctcctgc tcaggctcaa gaaggtcttg caaatgcctt
     6961 tgatggaaac ttatctagtt tatggcatac atcatggggc ggaggagatg taggcaagcc
     7021 tgcaaccatg gtcttgaaag aagcaactga aatcactgga cttcgttatg ttccacgtgg
     7081 atcaggttca aatggtaact tgcgtgatgt gaaacttgtt gtgacagatg agtctggcaa
     7141 ggagcatacc tttactgcaa ctgattggcc agataacaat aagccaaaag acattgattt
     7201 tggtaagaca attaaggcta agaaaattgt ccttacaggt actaagactt acggagatgg
     7261 tggcgataaa taccaatctg cagcggaact tatctttact cgtccacagg tagcagaaac
     7321 acctcttgac ttgtcaggct atgaagcagc tttggctaag gctcagaaat taacagacaa
     7381 agacaatcaa gaggaagtag ctagcgttca ggcaagcatg aaatatgcga cggataacca
     7441 tctcttgacg gaaagaatgg tggaatactt tgcagattat ctcaaccaat taaaagattc
     7501 tgctacgaaa ccagatgctc caactgtaga gaaacctgag tttaaactta gctctttagt
     7561 ttccaatcaa ggtaagacgc cagattataa gcaagaaata gatagaccag aaacacctga
     7621 acaaatcttg ccagcaacag gtgagagtca atctgacaca gccctcttcc tagcaagtgt
     7681 tagtctagcc ctatctgctc tctttgtagt aaaaacgaag aaagactagt atttagtaaa
     7741 acctcttaac aagattacgg aagcagtctc tatcttttcc aatgaggttt atagtacaga
     7801 aaaagcctga gaagatgtct tctcaggctt ttgttaagca cataaataca atagtgctat
     7861 gacaaaatca cccagaaaaa tctgggtgat aaatgttatg gttgtgctgg ttgaggattc
     7921 tgattttgtt gatcaggggt tgtatttgat tgttgcgtat tattgttagg attggtagtc
     7981 gtactattat ttgtgcttgg agtggttgag ctagactgtg aagttgaact atctgatgat
     8041 gagcttgaac tttcagttga tgggggttgt tgtggagcag gtgagttcca cgtagaacga
     8101 gcaccatttt taaatacgaa ttctccattt ctgtagagcc cctctggtat attccaatcc
     8161 tctggattgc ttccttcaga cagataggtc atcatagagc ggtaaacttt ggcagcgacc
     8221 gtaagaccat tgcctacaag tggtgtcaga cggttagaat agcctgtcca tacagccatt
     8281 gaatatttac gcgtatagcc agcaaatagt tcatcaggtg ctacaaattg agaggtcttg
     8341 atgtggtttt caatttcctc gtctgtatag ttagaggttc ctgttttacc agcctgaggg
     8401 agccaagcaa gataggcatt tcgtccagtt ccataactca agactgtttt catcatgtcg
     8461 gtcatcatat aggctgtcgt ttccttcatg gcacgagttc cgacattaga gaactctttt
     8521 tcactcccat cactaaagac gactttatgg atatacattg gtttatagta agttccacca
     8581 tttgcaaagg cagcgtaagc agcagccatc ttttcactac ttgctccata ttttttgtct
     8641 gattcggttg tgttacttga aatggcattt gagtagtgaa tacttgggta gtcgattccg
     8701 agaccattta ggaaagtctt ggcgcggttg agtccgacct tgtttagagt ttccacggct
     8761 gggacgtttc gcgattgttg cagggcgtat tgcaaggtga tgttgccaaa gtagccccta
     8821 tcccagttat aaacaggggt atttgtccca gggtagttat agggctcatc gtgaacgata
     8881 gtagcagttg aatcgtagat accgtactcc aaggcaggag catagtctgt gatcggtttc
     8941 atagttgatc cccagtcgcg gtttgtttct actgcttggt taattccgaa ggaaacatta
     9001 cttgactgat ggcgtgctcc tagctgggca atgactttac cgttagaaac atcaacaatg
     9061 gtagaagcga cttgcaattc atcgtctgga taggcaacgt attcgtctgt attgtaaata
     9121 tcccacagat gtttttgagc ttcttggtct acatttgtgt agacatccat cccagttgtg
     9181 agcaggttat agcctgtttc ttcttcaact tgattgatga cttccttgag gtaattatcc
     9241 atgtaagcag ggtaattact tgctgatttg agactttgta gtccatcagt aattggtgta
     9301 ttgactgctt tctcatactg ttcagcagag atgtagcctt gatttttcat ttcagataag
     9361 accaagtttc ggcggtcttg ggctgcttct ggatgtgaat aggggtcata ttggtttggt
     9421 gcctgaggca ttccagccag caaggctaac tgaggtaaac ttaaattatt gaggtcttta
     9481 ccatagtagt tttgagctgc tgtctgcatt ccatagttcc cattagacat gtagacctta
     9541 tttatatagt aggtcaagat ttcttgcttg gttgcttttt gttctaactg aatcgctaac
     9601 caagcttcct gagccttacg agaaatagtc tggtcggaag tcgaagttga aaagtaagtc
     9661 aacttaatca actgttgagt gagagttgat ccaccttgga gggaattgct ttgcagattg
     9721 cgcaagaaag ctcccaggat acggatggta tcaatccccc tgtggtcgaa gaagcgatgg
     9781 tcttcgatag aaacgattgc tttaaccaaa tctgtgggaa tatcattagc ttgggcattg
     9841 acgcggcgtt cagaacccaa gtcagcaatg agttgatttt tattgtcgta gattttacta
     9901 gaagttgttg caactagttt actctcggat aggctaggag ccttgctaac gtagtagaaa
     9961 aaaactcctc cgcctaagac aatggctgcg ataaccaagc ttaagaagct aatgctcaga
    10021 tacttgatta ggcgcagaat cgttggtttg ttcatcttgt tttaccacct aataaatgtt
    10081 ctttgataac attgagataa ggaatttgag ggaaggcacc agccttgatt tcatatccat
    10141 attctcgaat atattcaagt ggcattgatt tttgtccctt atcttgatga tagaagcgaa
    10201 tcaaatcgaa tgccggcaat aagtaggttt cttgctgaga agaaaagtga agaaggacaa
    10261 agcagattcc ttgttgggca aggacttgtt ccatatgctg aatctgatgt ggatgaaaat
    10321 ttttcatcgg aatcgcacgt ttttgttttg tttccttgac ttcaaagtcg atgtaatatc
    10381 cattataaac gccagaatag tccgtcgttg aagcttgtcg aaaataggct tcaacaatct
    10441 tggcacgact tcgttgtgga tagtccactt gtacgatttg aataggagtt ggtttcttat
    10501 gtataacagc caagccctga gacaaatagt agtcgttggt agcattgatc atcttttcaa
    10561 aagacattcc tcgatttgcg aaatttttgg gttgagaaag agatgtttgt cttttttgtg
    10621 atgaaacttt atgtggatag ttgaccataa ttctccttat tggtacaata acatcacttt
    10681 attataccat aatttgcaca gaaagggtta aaaatggcta cagctttggt tttaggctat
    10741 tctgcttttg atttgggttt attttctgat aaggatcctc gtcttaaatt gattaaaaaa
    10801 gcgatacgta aagatttaga ggctatggca gcagatgggg tgtcttggct tgtgtttaca
    10861 gggactttgg gctttgaata ttgggtttta gaggttgctc aggagatgaa aaccgaatac
    10921 ggtttccagt tggccaccat ttttgctttt gaaacccatg gggaaaattg gaatgaaggc
    10981 aatcagatga aactgagtcg ctttaagcag gtagactttg tcaaatatgc ctatcctcgc
    11041 tatgaacaca aggggcagtt aagagattac cagcagtttt tgctggaaaa cacgactagt
    11101 tcctatcttt tttatgatga agaaaatgaa acaaaactag cttattttta ccaaaagatg
    11161 aaaaatcaag aggactattt tataaagaga ttaacatttg atcaattaaa tgaacttgct
    11221 gaaaattttt ccgaaaattg aagctttgac cttgattttt gtttgccttt ttttatataa
    11281 taatactagc aagcgagaat ggagagagac atggcaagta ttattttttc agcgaaagat
    11341 atttttgaac aagagtttgg acgtgaagtc cgtggctata ataaagtaga agttgacgag
    11401 tttttagacg atgtcatcaa ggactatgaa acctatgctg ccttggtcaa gtcacttcgt
    11461 caggaaattg cggatttgaa ggaagaatta actcgtaaac cgaaaccttc accagttcaa
    11521 gcagaacccc ttgaagcggc aattacaagt tctatgacga attttgatat tttgaaacgc
    11581 ctgaatagat tggaaaaaga agtttttggt aaacaaattt tagataactc agatttttaa
    11641 gtagttattt gagatgtgca atttttggat aatcgcgtga ggagaattgc ttctcatgag
    11701 gaaagtccat gctagcacag gctgtgatgc ctgtagtgtt tgtgctaggc gaaaccataa
    11761 gcctagggac gagaaatcgt tacggcagtt gaaatggcta agtccttgga taggccagag
    11821 taggcttgaa agtgccacag tgacgaagtc tttctggaaa cagagagagt ggaacgcggt
    11881 aaacccctca agctagcaac ccaaattttg gtcggggcat ggagtacgcg gaaacgaacg
    11941 tagtattctg actgctatca gctagagctg ttagtggtag acagatgatt atcgaaggaa
    12001 gtggtcctag tcacttctgg aacaaaacat ggcttataga aaattgcata taggttgggg
    12061 ctgagaaatt ttctcaacct cattttttaa agtggacata tagaaaggtc ttgcaagact
    12121 gtaacatgaa aaaagaattt aatttaattg caactgtggc agcagggctt gaggctgtcg
    12181 ttggtcgtga agtgcgagag ttgggctacg attgtcagat tgaaaatgga cgtgttcgtt
    12241 ttcaaggaga cgtgagagct attatcgaaa ccaacctttg gctgcgggca gcagatcgta
    12301 tcaaaattat cgtaggaacg ttccctgcta agacttttga agagctattt cagggagttt
    12361 tcgctttaga ttgggaaaat tatttaccac ttggagctcg gttcccgatt tcaaaagcta
    12421 aatgtgttaa gtccaaactt cacaatgagc ccagtgttca ggctatttct aagaaagctg
    12481 ttgtcaagaa attgcagaaa cactatgctc gcccagaagg ggttcctctg atggagaatg
    12541 gcccagagtt taagattgag gtctctattc tcaaagatgt ggcaactgtc atgattgata
    12601 cgaccgggtc tagcctcttt aaacgtggtt atcgtaccga aaaaggtggc gctcctatca
    12661 aggaaaatat ggcagcagcc attttacaac tttctaactg gtatccagac aagcctttga
    12721 ttgatccgac ctgtggttcg gggactttct gtattgaggc agttatgatt gctagaaaga
    12781 tggcgccagg tcttcgtcgc tcttttgcat ttgaggaatg gaactggatc agcgatcgct
    12841 tgattcaaga agtgcgcaca gaagcggcta aaaaagtaga ccgtgagctt gagctggata
    12901 tcatgggctg tgatattgat gctcgcatgg tggaaattgc taaggccaat gctcaggcag
    12961 ctggtgttgc aggagacatt acttttaagc agatgcgcgt gcaggattta tgttccgata
    13021 aaatcaatgg agtaatcatt tccaatccgc cttatggtga acgtttgtca gatgatgcag
    13081 gggtgaccaa gctctatgct gagatggggc aagtatttgc accgctgaaa acttggagca
    13141 aatttatcct gactagtgat gaagcttttg aaagcaagta tggtagccaa gcagataaga
    13201 agcgtaagtt atacaacgga accttgaaag tggatctgta tcaatatttt ggtcagcgtg
    13261 tcaaacggca agaggtaaaa tagaaaggga tactcatgag taaaaaaaga cgaaatcgtc
    13321 aaaaaaaaga aggtcaagaa ccgcaatttg attttgatga agcaaaagag ctaacagttg
    13381 gtcaagctat tcgtaaaaat gaagaagtgg aagcaggagt cttgcctgag gattccattt
    13441 tggacaagta tgttaagcaa cacagagatg aaattgaggc ggataagttt gcgactcgtc
    13501 aatacaaaaa agaggagttc gttgaaactc agagtctgga tgatttaatt caagagatgc
    13561 gtgaggctgt agagaagtca gaagcttctt cggaggaagt tccatcttct gaagacatct
    13621 tactaccctt gcctctggac gatgaggagc aaggcttgga tcctctattg ctagatgatg
    13681 aaaatccaac agaaatgact gaagaagtgg aagaggagca aaacctttct cgtctggatc
    13741 aagaggactc agaaaagaaa agtaaaaaag gctttatttt gaccgttttg gcgcttgtat
    13801 cagtaattat ttgtgtcagt gcttattatg tctaccgtca agtggctcgt tcgaccaagg
    13861 aaattgaaac ttctcaatca actacagcca atcaatcgga tgtggatgat tttaatacac
    13921 tttatgacgc cttttacaca gatagcaata aaacggcttt gaaaaatagc cagtttgata
    13981 aactgaatca actcaagact ttacttgata agctggaagg tagtcgtgaa catacgcttg
    14041 ccaaatctaa atatgatagt ctagcaacgc aaatcaaggc tattcaagat gtcaatgctc
    14101 aatttgagaa accagctatt gtggatggtg tgttggatac caatgccaaa gccaaatcgg
    14161 atgctaaatt tacggatatt aaaactggaa atacggagct tgataaagtg ctagataagg
    14221 ctatcagtct tggtaagagc cagcaaacaa gtacttctag ctcaagttca agtcaaacta
    14281 gcagctcaag ttcaagtcaa gcaagttcaa atacgactag tgagccaaaa ccaagtagtt
    14341 caaatgagac tagaagtagt cgcagtgaag tcaatatggg tctctcgagt gcaggggttg
    14401 ctgttcaaag aagtgccagt cgtgttgcct ataatcagtc tgctattgat gatagtaata
    14461 actctgcctg ggattttgcg gatggtgtct tggaacaaat tctagcgact tcacgttcac
    14521 gtggctatat cactggagac caatatatcc ttgaacgtgt caatatcgtt aacggcaatg
    14581 gttattacaa cctctataag ccagatggaa cctatctctt tacccttaac tgtaagacag
    14641 gctactttgt cggaaatggc gctggtcatg cggatgactt ggactactaa gcagtcgtta
    14701 caaaattctt tcttttcaaa agtaaaaatg ataaaataaa acaaatgaaa caagaggagt
    14761 gtcaaatgac aaaagctaac tttggtgtcg taggtatggc cgtaatgggt cgtaaccttg
    14821 cccttaatat tgaatcacgt ggttacacag ttgctatcta caaccgtagt aaagaaaaaa
    14881 cggaagatgt gattgcttgc catcctgaaa agaattttgt accaagctat gacgttgaaa
    14941 gttttgtaaa ctcaatcgaa aaacctcgtc gtatcatgct gatggttcaa gctggacctg
    15001 gtacagatgc tactatccaa gcccttcttc cacaccttga caagggtgat atcttgattg
    15061 acggaggaaa cactttctac aaagatacca tccgtcgtaa tgaagaattg gcaaactcag
    15121 gtatcaactt tatcggtact ggagtttctg gtggtgaaaa aggtgccctt gaaggtcctt
    15181 ctatcatgcc tggtggacaa aaagaggcct acgaattggt tgcggatgtt cttgaagaaa
    15241 tctcagctaa agcaccagaa gatggcaaac catgtgtgac ttacatcggc cctgatggag
    15301 ctggtcacta tgtgaaaatg gttcacaatg gtattgagta cggtgatatg caattgatcg
    15361 cagaaagcta tgacttgatg caacacttgc taggcctttc tgcagaggat atggctgaaa
    15421 tctttactga gtggaacaag ggtgaattag acagctattt gatcgaaatc acagctgata
    15481 tcttgagcca taaagacgat gaaggccaag atggaccaat cgtagactac atccttgatg
    15541 ctgcaggtaa caagggaact ggtaaatgga ctagccaatc atctcttgac cttggtgtac
    15601 cattgtcact gattactgag tcagtgtttg cacgctacat ttcaacttac aaagaagaac
    15661 gtgtatatgc tagcaaggtg cttccaaaac cagctgcctt caactttgaa ggagacaagg
    15721 ctgaattgat tgaaaagatc cgtcaagccc tttacttctc aaaaatcatt tcatacgcac
    15781 aaggatttgc tcaattgcgt gtagcctcta aagaaaacaa ctggaacttg ccatttgcag
    15841 atatcgcatc tatctggcgt gatggctgta tcatccgttc tcgtttcttg caaaagatta
    15901 cagatgctta caaccgcgat gcagatcttg ccaaccttct tttggacgag tacttcttgg
    15961 atgttactgc taagtatcaa caagcagtgc gtgatatcgt agctcttgcg gttcaagctg
    16021 gtgtgccagt gccaactttc tcagcagcta ttacttactt tgatagctac cgttcagctg
    16081 accttccagc taacttgatc caagcacaac gtgactactt tggtgctcac acttaccaac
    16141 gtaaagacaa agaaggaacc ttccactact cttggtatga cgaaaaataa gtaggtcagc
    16201 catggggaaa cggattttat tacttgagaa agaacgaaat ctagctcatt ttttaagttt
    16261 ggaactccag aaagagcagt atcgggttga tctggtagag gaggggcaaa aagccctctc
    16321 catggctctt cagacagact atgatttgat gttattgaac gttaatctgg gagatatgat
    16381 ggctcaggat tttgcagaaa aattgagtcg aactaaacct gcctcagtca tcatgatttt
    16441 agatcattgg gaagacttgc aagaagagct ggaagttgtt cagcgttttg cagttgcata
    16501 catttataag ccagtcctta tcgaaaatct ggtagcgcgt atttcggcga tcttccgagg
    16561 tcgggacttc attgatcaac actgcagtct gatgaaagtt ccaaggactt accgcaatct
    16621 taggatagat gttgaacatc acacggttta tcgtggtgaa gagatgattg ctctgacccg
    16681 ccgtgagtat gaccttttgg cgacacttat gggaagcaag aaagtattga ctcgtgagca
    16741 attgttggaa agtgtttgga agtatgaaag tgtgaccgag acaaatatcg tagatgtcta
    16801 tatccgctat ctacggagca agcttgatgt taaaggccaa aaaagctaca ttaaaaccgt
    16861 gcgtggtgtt ggatatacca tgcaagaata gataagcagt tgcagttgtg taactgcttt
    16921 tttgaggagt ttttatatat tgacatgcag tcgggtcttt gctacaatcg ttttaggagg
    16981 atatatagat gaaacttttg aaaaaaatga tgcaagttct actagcagtc tttttctttg
    17041 gtttgctagc tacaaatacg gtatttgcga ataccacagg tggccaattt gttgataagg
    17101 ataatagaaa atattatgta aaagatgatc ataaagcaat ctattggcat aaaatagacg
    17161 gtaaaactta ctattttggt gatattggag agatggttgt cggttggcaa tacttagaaa
    17221 ttcctggaac aggttatcgt gataatttat tcgataacca accagttaat gagattggcc
    17281 ttcaggagaa gtggtactat tttggacaag atggtgcttt gctagaacaa acagataaac
    17341 aagtactaga gccaaaaacg tctgaaaata caggaaaagt atacggtgaa caatatcctc
    17401 tatctgctga aaagagaact tattattttg ataataatta tgctgtaaag acaggctgga
    17461 tttatgaaga cggcaattgg tattatttaa ataagctagg aaattttggc gatgattctt
    17521 acaatccact accaattggt gaagttgcta agggttggac tcaagatttt catgttacta
    17581 ttgacattga tagaagcaaa cctgctccat ggtactacct agatgcttca ggtaagatgc
    17641 ttacagattg gcaaaaagta aacggaaaat ggtattattt tggctcctct ggttctatgg
    17701 caacaggttg gaaatatgta cgaggcaaat ggtattactt agataataaa aatggtgata
    17761 tgaaaacagg atggcaatac tttggtaaca agtggtacta cctaaatgca ggtaatggag
    17821 acatgacgac aggttggttc caggtcaatg gcagatggta ctacgcttat agctcaggtg
    17881 ccttggcagt gaatacgacc gtagatggct attctgtcaa ctataatggc gaatgggttc
    17941 aataatgaaa gaagcgattg tgaatggaaa caatcgcttt ttttgtgaaa atataataaa
    18001 atagatagga aagaattaat acttgtatga aaaataagac gttttttcgt ctagtaaaag
    18061 gaaaaaatga caaaaaaagt tggtgtcggt caggcacata gtaagataat tttaataggg
    18121 gaacatgcgg tcgtttacgg ttatcctgcc atttccctgc ctcttttgga ggtggaggtg
    18181 acctgtaagg tagttcctgc agagagtcct tggcgccttt atgaggagga taccttgtcc
    18241 atggcggttt atgcctcact ggagtatttg aatatcacag aagcctgcat tcgttgtgag
    18301 attgactcgg ctatccctga gaaacggggg atgggttcgt cagcggctat cagcatagcg
    18361 gccattcgtg cagtatttga ctactatcag gctgatctgc ctcatgatgt actagaaatc
    18421 ttggtcaatc gagctgaaat gattgcccat atgaatccta gtggtttgga tgctaagacc
    18481 tgtcttagtg accaacctat tcgctttatc aagaacgtag gatttacaga acttgagatg
    18541 gatttatccg cctatttggt gattgccgat acgggtgttt atggtcatac tcgtgaagcc
    18601 atccaagtgg ttcaaaataa gggcaaggat gccctaccgt ttttgcatgc cttgggagaa
    18661 ttaacccagc aagcagaagt tgcgatttca caaaaagatg ctgaaggact gggacaaatc
    18721 ctcagtcaag cgcatttaca tttaaaagaa attggagtca gtagccctga ggcagacttt
    18781 tgggttgaaa cgactcttag ccatggtgct ctgggtgcca agatgagcgg tggtgggcta
    18841 ggaggttgta tcatagcctt ggtaaccaat ttgacacacg cacaagaact agcagaaaga
    18901 ttagaagaga aaggagctgt tcagacatgg atagagagcc tgtaacagta cgttcctacg
    18961 caaatattgc tattatcaaa tattggggaa agaaaaaaga aaaagagatg gtgcctgcta
    19021 ctagcagtat ttctctaact ttggaaaata tgtatacaga gacgaccttg tcgcctttac
    19081 cagccaatgt aacagctgac gaattttaca tcaatggtca gctacaaaat gaggtcgagc
    19141 atgccaagat gagtaagatt attgaccgtt atcgtccagc tggtgagggc tttgtccgta
    19201 tcgatactca aaacaatatg cctacggcag cgggtctgtc ctcaagttct agtggtttgt
    19261 ccgccctggt caaggcttgt aatgcttatt tcaagcttgg attggataga agtcagttgg
    19321 cacaggaagc caaatttgcc tcaggttctt cttctcggag tttttatgga ccactaggag
    19381 cctgggataa ggatagtgga gaaatttacc ctgtagagac agacttgaaa ctagctatga
    19441 ttatgttggt gctagaggac aagaaaaaac caatctctag ccgtgacggg atgaaacttt
    19501 gtgtggaaac ctcgacgact tttgacgact gggttcgtca gtctgagaag gactatcagg
    19561 atatgctgat ttatctcaag gaaaatgatt ttgccaagat tggagaatta acggagaaaa
    19621 atgccctggc tatgcatgct acgacaaaga ctgctagtcc agccttttct tatctgacgg
    19681 atgcctctta tgaggctatg gactttgttc gccagcttcg tgagaaagga gaggcctgct
    19741 actttaccat ggatgctggt cccaatgtta aggtcttctg tcaggagaaa gacttggagc
    19801 atttatcaga aattttcggt catcgttatc gcttgattgt gtcaaaaaca aaggatttga
    19861 gtcaagatga ttgctgttaa aacttgcgga aaactctatt gggcaggtga atatgctatt
    19921 ttagagccag ggcagttagc tttgataaag gatattccca tctatatgag ggctgagatt
    19981 gctttttctg acagctaccg tatctattca gatatgtttg atttcgcagt ggacttaagg
    20041 cctaatcctg actacagctt gattcaagaa acgattgctt tgatgggaga cttcctcgct
    20101 gttcgcggtc agaatttaag acctttttcc ctaaaaatct gtggcaaaat ggaacgagaa
    20161 gggaaaaagt ttggtctagg ttctagtggc agcgtcgttg tcttggttgt caaggcttta
    20221 ttggctctct ataatctttc ggttgatcag gagctcttgt tcaagctgac tagcgctgtc
    20281 ttgcttaagc gaggagacaa tggttccatg ggcgaccttg cctgtattgt ggcagaggat
    20341 ttggttctct accagtcatt tgatcgccag aaggcggctg cttggttaga agaagaaaac
    20401 ttggcgacag ttctggagcg tgattgggga ttttttatct cacaagtgaa accaacttta
    20461 gaatgtgatt tcttagtggg atggaccaag gaagtggctg tatcgagtca catggtccag
    20521 caaatcaagc aaaatatcaa tcaaaatttt ttaagttcct caaaagaaac ggtggtttct
    20581 ttggtcgaag ccttggagca ggggaaagcc gaaaaagtta tcgagcaagt agaagtagcc
    20641 agcaagcttt tagaaggctt gagtacagat atttacacgc ctttgcttag acagttgaaa
    20701 gaagccagtc aagatttgca ggccgttgcc aagagtagtg gtgctggtgg tggtgactgt
    20761 ggcatcgccc tgagttttga tgcgcaatca accaaaacct taaaaaatcg ttgggccgat
    20821 ctggggattg agctcttata tcaagaaagg ataggacatg acgacaaatc gtaaggacga
    20881 gcatatcctc tatgcccttg agcagaaaag ttcctataat agctttgatg aggtggagct
    20941 gattcattct tccttgcctc tttacaatct ggatgaaatc gatctttcga cagagtttgc
    21001 tggtcgaaag tgggactttc ctttttatat caatgccatg actggtggaa gtaataaggg
    21061 aagagaaatc aatcaaaagc tggctcaggt ggcggaatcc tgtggtattt tatttgtaac
    21121 gggttcttat agcgcagccc tcaaaaatcc aacggataat tctttttctg tcaagtctag
    21181 tcatcccaat ctcctccttg gaaccaatat tggattggac aaacctgtcg agttaggact
    21241 tcagactgta gaagagatga atcctgttct attgcaagtg catgtcaatg tcatgcagga
    21301 attactcatg cccgagggag aaaggaagtt tagaagctgg caatcgcatc tagcagatta
    21361 tagcaagcaa attcccgttc ctattgtcct caaggaagtg ggctttggaa tggatgccaa
    21421 gacaatcgaa agagcctatg aattcggtgt tcgtacagtg gacctatcgg gtcgtggtgg
    21481 caccagcttt gcctatatcg aaaaccgtcg tagtggccag cgtgattacc tcaatcaatg
    21541 gggtcagtct accatgcagg cccttctcaa tgcccaagaa tggaaagata aggtcgaact
    21601 cttggttagt ggaggggttc ggaatccgct ggatatgatt aagtgcttgg tttttggtgc
    21661 taagtctgtg ggattgtcac gaaccgttct ggaattggtt gaaacctaca cagttgaaga
    21721 agtgattggc attgtccaag gctggaaagc agacctacgc ttgattatgt gttcccttaa
    21781 ctgtgccacc atagcagatc tacaaaaagt agactatctt ctttatggaa aattaaaaga
    21841 agcaaatgat cagatgaaaa aggcgtaacc agcgcctttt ttccatcctc agactgaggt
    21901 gacttttttg aattgtgata aaatagaagg gagaggatga acctatgaga aaatttaaaa
    21961 tctttttatt tatcgaagcc tgtcttctga caggagctct gattttgatg gtatcagagc
    22021 atttttcgcg ttttctgctg atactattcc tctttttgct tttgattcgc tactacactg
    22081 gtaaagaggg gaataatctt cttttagtag cggcaaccat tctcttcttt ttcattgtta
    22141 tgctcaatcc ttttgtgatt ctagctattt ttgttgcggt tatctatagt ctctttcttc
    22201 tttacccgat gatgaaccag gaaaaagagc agaccaattt ggtttttgaa gaggttgtga
    22261 cggttaagaa ggagaaaaat cgttggtttg gaaatcttca tcatttttca agctaccaga
    22321 cttgccaatt tgatgatatc aatctctttc gcttcatggg caaggacact attcatctgg
    22381 agagagtcat cttaaccaat catgacaatg tcattatcct cagaaagatg gtaggaacga
    22441 ccaaaatcat cgtacctgta gatgtggaag tcagtctcag cgttaactgt ctctatgggg
    22501 atttgacttt tttcaaccag cccaagcgag ccctccgcaa tgaacactat catcaagaaa
    22561 caaaagacta tctcaagagt aacaagagtg tcaagatttt cttgaccact atgattggtg
    22621 atgttgaggt ggttagagga tgaaaaaaca agcctatgtc attatcgctc tcacctcctt
    22681 cctgtttgtc ttttttttct cccatagctt gctggaaata cttgattttg actggtctat
    22741 ctttttgcac gatgtcgaaa aaacagaaaa atttttcttt ttattgttgg ttttcagcat
    22801 gtccatgacc tgtctcttag ccctgttttg gcgagggatc gaagagcttt ctctaagaaa
    22861 aatgcaggct aatctcaagc gtttattggc agggcaagaa gtggttcagg ttgtagatcc
    22921 agatttggat gccagtttca agtccttatc aggtaaactt aaccttttga cagaggctct
    22981 tcaaaaagct gaaaatcata gccttgctca ggaagaggaa atcatcgaga aggaacggaa
    23041 gcgaattgct cgggatttgc acgatacagt cagtcaggag ttgtttgcgg cccacatgat
    23101 tttatcgggt atcagtcagc aggctttgaa attggataga gaaaagatgc agacccagtt
    23161 gcagagtgtc acagctattt tagaaacagc ccagaaggat ttgcgggttt tgctcttgca
    23221 tttgcgacca gttgaactgg agcagaagag cttgatagaa gggattcaaa ttcttttaaa
    23281 agagcttgag gacaagagtg atcttagggt tagtctcaag cagaatatga cgaaattgcc
    23341 taagaaaatc gaggagcata tcttccgtat cctgcaagag ttgattagca ataccctccg
    23401 ccatgcccag gcatcttgcc tagatgtcta cctctatcag acagatgttg aattgcaact
    23461 gaaggtggtg gacaatggga ttggtttcca gttagggagc ttagacgact tgagttatgg
    23521 actgcgaaat atcaaggagc gggttgaaga tatggctgga acagttcaac tcttgacagc
    23581 tcccaagcaa gggctggcgg ttgatatccg tattcctctg ttagataagg aatgataaag
    23641 gagtaaagat gaaaatttta ctagtagatg accatgaaat ggtccgattg ggcttgaaaa
    23701 gctactttga cctccaagac gatgtagaag ttgtgggtga ggcgtccaac gggtctcaag
    23761 ggattgactt ggccttggaa ctgcgtccag atgtcattgt catggatatt gtcatgcctg
    23821 agatgaatgg gattgacgcg accttagcaa tccttaaaga atggcctgaa gccaagattt
    23881 tgattgtgac ctcttatttg gacaatgaaa aaatcatgcc agtcttagat gctggtgcca
    23941 aaggctatat gctcaagact tctagtgcag atgaattgct tcatgccgtc agtaaggtag
    24001 ctgctggcga gctggccatt gagcaagagg ttagcaagaa ggttgaatac caccgcaatc
    24061 atatggaact tcatgaagaa ttgactgcgc gtgagcgaga tgttctccaa ctcatcgcca
    24121 agggctacga aaatcagcgc atcgcagatg acctctttat ctctctcaag acggtcaaga
    24181 cccacgtgtc caatattctt gccaaacttg aagtcagcga tcgtacccag gcggctgtct
    24241 atgcctttca gcaccatttg gtggggcaag aggagtttta gatgagttta gcagatttac
    24301 ttgaggagct agaagcagca aaagactcta aaaaagcaag gtccatggaa gcctatatgc
    24361 gccatcaatt ttcttttcta ggtattgcgg tgcctgaaag aaataaactc tataaaatat
    24421 tttccagaag cgaaaaaaac aaagattatc gattgggatt ttgcagacac ttgctgggaa
    24481 aagaatccta gaaaatacca atatgtggct gctaactatt tgaaagcaat gcagtcttat
    24541 ctaaaggaaa ccgatttgcc taagctagag cagttagtcg tgacaaaatc ctggtgggac
    24601 acggtagata tcctagatcg agtagtaggg agtttggtgt atgaacactc tgaactagaa
    24661 gaaataatct taatactctt cgaaaatctc ttcaaaccac gtcagctcta tctgcaacct
    24721 caaaacagtg ttttgagcaa tctgcggcta gcttcctagt ttgctctttg attttcattg
    24781 agtataaaat ggagcctatc agataatatc tggctgagac gtgtcgctat tgaccaccag
    24841 ttgttaagaa aagagaagac caatactcaa ttaatggaaa agattctact tcataacttg
    24901 aaccaaacag aattttttat caataaagcc attggctgga ctctgcgaga ctactccaaa
    24961 acgaatccca cttgggtaac atgctttatt gagaaaaaca aggaaagaat ggctgaactt
    25021 agtatcaaag aagcaagcaa gtacctctag cgtgattgaa aagcgcagtc attaattgaa
    25081 aagtttacct gtttatgtga taatggaggg tatttaaaga aggtggtaga tttcttgatt
    25141 ctatagtaga ttaagaaatt tagaatagta cgctataatt acattaggag gatacaataa
    25201 tgaagaaaac tgtttataaa aaattgggta tttcaattat tgcgagtact ttattggcta
    25261 gccagttatc gacagtatct gctttgagtg ttatttctag tacaggtgaa gaatatgagg
    25321 taagtgagac actagaaaaa ggtccagagt ctaatgattc ttcattatct gagatttcac
    25381 caacgtatgg ttcatactac caaaagcaat cagaagtatt atcggtaatg atgatttgat
    25441 taaaattgat aatactttac agtatccgta ttcgacttca gctatggttt tatctaagta
    25501 ttatggagta gccgatggaa tgaatgtaga agggagggga agtgcgaatt ttattaaaga
    25561 taatgtgtta attacagcgg ctcacaacta ctacagacat gactatggga aagaagcgga
    25621 tgatatttat gttcttccgg ctgttagtcc aagtcaagaa ctatttggaa agatcaaagt
    25681 aaaggaagtt cgttatttga aggaatttag aaatttaaat tctaaggatg caagggaata
    25741 tgacttggct ttattaattc tagaagagcc cattggtgca aaattaggga ctttgggtct
    25801 tcctactagt caaaaaaatt tgacaggaat aactgtgact atcacaggct atccatcata
    25861 taattttaaa attcatcaaa tgtatacaga taaaaaacaa gttttaagtg atgatggcat
    25921 gttcttggat taccaagttg atactttaga ggggtctagt ggatctacag tttatgatgc
    25981 tagtcaccgt gtagtaggag tgcatacttt aggagatgga gctaatcaaa ttaacagtgc
    26041 agttaaatta aatgaacgaa atttgccatt tatttattcg gttcttaaag gttactctct
    26101 tgaaggatgg aagaaaataa atggtagttg gtaccattat agacaacatg ataaacaaac
    26161 gggttggcag gagataaatg atacttggta ttatttagac agttccggta agatgcttac
    26221 agattggcaa aaagtaaatg gaaaatggta ttatctcaat tcaaatggag caatggttac
    26281 aggtagccaa actatcgatg gtaaagttta taacttcgct tcatctggtg agtggattta
    26341 atgttggagg atatataaaa tgaagctttt gaaaaaaatg atgcaaatcg cactagccac
    26401 atttttcttc ggtttgttag cgacaaatac agtatttgca gatgattctg aaggatggca
    26461 gtttgtccaa gaaaatggta gaacctacta caaaaagggg gatctaaaag aaacctactg
    26521 gagagtgata gatgggaagt actattattt tgatccttta tccggagaga tggttgtcgg
    26581 ctggcaatat atacctgctc cacacaaggg ggttacgatt ggtccttctc caagaataga
    26641 gattgctctt agaccagatt ggttttattt tggtcaagat ggtgtattac aagaatttgt
    26701 tggcaagcaa gttttagaag caaaaactgc tacgaatacc aacaaacatc atggggaaga
    26761 atatgatagc caagcagaga aacgagtcta ttattttgaa gatcagcgta gttatcatac
    26821 tttaaaaact ggttggattt atgaagaggg tcattggtat tatttacaga aggatggtgg
    26881 ctttgattcg cgcatcaaca gattgacggt tggagagcta gcacgtggtt gggttaagga
    26941 ttaccctctt acgtatgatg aagagaagct aaaagcagct ccatggtact atctaaatcc
    27001 agcaactggc attatgcaaa caggttggca atatctaggt aatagatggt actacctcca
    27061 ttcgtcagga gctatggcaa ctggctggta taaggaaggc tcaacttggt actatctaga
    27121 tgctgaaaat ggtgatatga gaactggctg gcaaaacctt gggaacaaat ggtactatct
    27181 ccgttcatca ggagctatgg caactggttg gtatcaggaa agttcgactt ggtactatct
    27241 aaatgcaagt aatggagata tgaaaacagg ctggttccaa gtcaatggta actggtacta
    27301 tgcctatgat tcaggtgctt tagctattaa taccacagta ggtggttact atttaaacta
    27361 taatggtgaa tgggttaagt aatgaagact aattgtaaac tgtgatggat acttaacttt
    27421 gtataatagg tggataaaag tcttcacaat caaaaaacgc atagtatcaa ggtttttctg
    27481 tactgccccc caaaagttag acaaataatt tatccgaagg atttagttct gtattgcaca
    27541 gggctaagtc cttttagttt taccttaatt cgtttattgt tgtagtaatc aatatagtct
    27601 acaatagctt gttccaattg attaagtgat ttaaatgttt tctcatagcc ataaatttca
    27661 gaatcccaaa gaaggactcc atcataccgt tgtcttggct gtttcccttg cgtgacatgg
    27721 atgcttgaat tcccttactc tctaagaacc gatgataaga atcgtgttga tattgccagc
    27781 cttggtcact atggagaatc gtattctcat agtatttctc tgtaaaggcc tgttctaaca
    27841 tactcttcac ttgttctaag ttgggtgaag ttgaaagatt ataggcgata atttcgctgt
    27901 taaagccatc taaaactggt gataagtaaa gcttttgagt acttgctgga atggcaaatt
    27961 ctgtcacatc tgtgtagcac ttttccattg ttttagagcc ttcaaattgg ccttgaatga
    28021 gattctctgc cttcttacca acgtctcctt tattttcgtt tctgtcacat tttagcttgt
    28081 aaattgagta ctttcatcaa gtcttgaact cttttatgat ttaccagata agcacgattt
    28141 cttagttcta aataaattcg acgataagca taatttccct tgtgttcgat aaagatggat
    28201 tgaatttcag ctttaagctc ttggtcctta tctggtttat ctagctgttt caagtgatag
    28261 tagtaggtcg aacgagctag tttaatggct tttagaagaa gatctaacga aaactcagtc
    28321 attaattctt gaacaatttc tgtctttctt ctttctcttt ttcctccttc aatcggagtt
    28381 ctcttaactt ttttaggatg gcattctccg atctcaggta ctctctctct tgttttctca
    28441 acaatagtat acccgttttt cctgtattgt gctagccagt taagaagtat cgtacgactt
    28501 gggaggcagt attcaagaga
//
LOCUS       DAVLRT010000019        28158 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLRT010000019 DAVLRT010000000
VERSION     DAVLRT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.019"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..659
                     /locus_tag="VXM85_000824"
     CDS             <1..659
                     /locus_tag="VXM85_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1345438.1"
                     /translation="LLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            1084..1317
                     /locus_tag="VXM85_000825"
     CDS             1084..1317
                     /locus_tag="VXM85_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1345439.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            1600..3549
                     /gene="mutL"
                     /locus_tag="VXM85_000826"
     CDS             1600..3549
                     /gene="mutL"
                     /locus_tag="VXM85_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1345440.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3874..4341)
                     /locus_tag="VXM85_000827"
     CDS             complement(3874..4341)
                     /locus_tag="VXM85_000827"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1345441.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4342..5547)
                     /locus_tag="VXM85_000828"
     CDS             complement(4342..5547)
                     /locus_tag="VXM85_000828"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1345442.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5567..6202)
                     /gene="ribE"
                     /locus_tag="VXM85_000829"
     CDS             complement(5567..6202)
                     /gene="ribE"
                     /locus_tag="VXM85_000829"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1345443.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(6187..7287)
                     /gene="ribD"
                     /locus_tag="VXM85_000830"
     CDS             complement(6187..7287)
                     /gene="ribD"
                     /locus_tag="VXM85_000830"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1345444.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7421..7535)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7692..8285
                     /gene="ruvA"
                     /locus_tag="VXM85_000831"
     CDS             7692..8285
                     /gene="ruvA"
                     /locus_tag="VXM85_000831"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1345445.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8295..8858
                     /locus_tag="VXM85_000832"
     CDS             8295..8858
                     /locus_tag="VXM85_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1345446.1"
                     /translation="MTKRCSWVKMTNPLYITYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8855..9532
                     /locus_tag="VXM85_000833"
     CDS             8855..9532
                     /locus_tag="VXM85_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1345447.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALIHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9669..10699)
                     /locus_tag="VXM85_000834"
                     /pseudo
     CDS             complement(9669..10699)
                     /locus_tag="VXM85_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
     gene            complement(10954..11637)
                     /locus_tag="VXM85_000835"
     CDS             complement(10954..11637)
                     /locus_tag="VXM85_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1345448.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11649..12593)
                     /locus_tag="VXM85_000836"
     CDS             complement(11649..12593)
                     /locus_tag="VXM85_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1345449.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12725..15556
                     /gene="uvrA"
                     /locus_tag="VXM85_000837"
     CDS             12725..15556
                     /gene="uvrA"
                     /locus_tag="VXM85_000837"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1345450.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILASVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15549..16610
                     /locus_tag="VXM85_000838"
     CDS             15549..16610
                     /locus_tag="VXM85_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1345451.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASSLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16742..17140
                     /gene="spx"
                     /locus_tag="VXM85_000839"
     CDS             16742..17140
                     /gene="spx"
                     /locus_tag="VXM85_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1345452.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            17206..17775
                     /locus_tag="VXM85_000840"
     CDS             17206..17775
                     /locus_tag="VXM85_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1345453.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGTATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17862..18128
                     /locus_tag="VXM85_000841"
     CDS             17862..18128
                     /locus_tag="VXM85_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1345454.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            18132..18551
                     /gene="ruvX"
                     /locus_tag="VXM85_000842"
     CDS             18132..18551
                     /gene="ruvX"
                     /locus_tag="VXM85_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1345455.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18567..18872
                     /locus_tag="VXM85_000843"
     CDS             18567..18872
                     /locus_tag="VXM85_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1345456.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            19131..20381
                     /locus_tag="VXM85_000844"
     CDS             19131..20381
                     /locus_tag="VXM85_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1345457.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKDGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMREGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20378..20920
                     /locus_tag="VXM85_000845"
     CDS             20378..20920
                     /locus_tag="VXM85_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1345458.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            21066..22598
                     /gene="cls"
                     /locus_tag="VXM85_000846"
     CDS             21066..22598
                     /gene="cls"
                     /locus_tag="VXM85_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1345459.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIMEIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22676..22819
                     /locus_tag="VXM85_000847"
     CDS             22676..22819
                     /locus_tag="VXM85_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345460.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            22856..24394
                     /locus_tag="VXM85_000848"
     CDS             22856..24394
                     /locus_tag="VXM85_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1345461.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     YFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILY
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24514..26721
                     /gene="nrdD"
                     /locus_tag="VXM85_000849"
     CDS             24514..26721
                     /gene="nrdD"
                     /locus_tag="VXM85_000849"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1345462.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTD"
     gene            26733..26873
                     /locus_tag="VXM85_000850"
     CDS             26733..26873
                     /locus_tag="VXM85_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345463.1"
                     /translation="MGKYQLDDKGCAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26917..27423
                     /locus_tag="VXM85_000851"
     CDS             26917..27423
                     /locus_tag="VXM85_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1345464.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            27416..28006
                     /gene="nrdG"
                     /locus_tag="VXM85_000852"
     CDS             27416..28006
                     /gene="nrdG"
                     /locus_tag="VXM85_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1345465.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            28014..>28158
                     /locus_tag="VXM85_000853"
     CDS             28014..>28158
                     /locus_tag="VXM85_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345466.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTV"
BASE COUNT         8299 a         5330 c         6105 g         8424 t
ORIGIN      
        1 tattgttgtt gttaaaattt agaactagca atgcgaatat agaaaacttc tctttcaaat
       61 attacaagag agaagtgttg caaggtacaa agtttatttt aaataataaa ttattattta
      121 aaaccagtat ttctttaacg cttataaact ttttttattc atttcagaca gtagttgtac
      181 cgattttttc tattcgatat tttgatggtc cgatttttta tggtattttt ttaactattg
      241 ctggtttggg tggtatattg ggaaatatgc tagcgccaat cgtaataaaa tatttaaaat
      301 cgaatcaaat tgttggtgta tttctttttt tgaacggctc aagttggtta gtagcaattg
      361 ttataaaaga ctatacttta tcacttattt tatttttcgt ttgttttatg tctaaaggag
      421 tcttcaatat tatttttaat tcgttgtacc aacaaatacc tccacatcaa cttcttggta
      481 gggtaaatac taccattgat tctattattt cttttggaat gccaattggt agtttagttg
      541 caggaacgct tattgatttg aatattgaat tagtgttaat tgctattagc ataccttatt
      601 ttttgttttc ttatattttt tatacggata atggattgaa agaatttagt atatattaga
      661 aatgtttatg ttcattcaaa agcataatga ctataactga aaaagaaaag tgatatcttt
      721 aaggttgttc ttcttggtgg tgagattcgt gagacaaccc aagcttttgt cggaaagatt
      781 accaatgctt tgatggatag gatgtacttt agcaagatgt ttttagtggt aacggtatcg
      841 tggatggacg tgtaataacc tcttctttcg aggagtattt tactaaaaaa ctagccttgg
      901 agcgttcccc agaaacggac ttactcattg actcttcaaa gatttgggga gaagattttg
      961 cttcatctgt tccttgaaaa aagtcacagc agtcatcaca gacgatagta ctgaacaaaa
     1021 ctatgaagag ttagaaattt atacgcaggt gattgtataa aggatctgga aatagataag
     1081 aagttgatta gtattgacct aggtggtaca aatattaaga ttactgttct ttcaaatgac
     1141 ggtgagattg aaactttgtg gagtattaca acagatacaa gtgagaaagg ttctcaaatt
     1201 atatcggaca tcatcagttc tattaaaaat aaattgaccg aacggaatat tcctgatagc
     1261 gaccttcttg gaatcggtat gggaagttgc tcatcatact ttccttgtaa atcatagggg
     1321 ctataaactc tccgtctact tgtcctgcaa caattgaagt ctgctcaaaa cgccgtccgc
     1381 taatcttttc atagactttc tcccttttag gagcctagct ttctagtttg ttctttgatt
     1441 tttattgagt ataccactat tttactccct ctggcaaggg actttgtcta tgtggaggga
     1501 ttgggctcct atgtggtgga gcttttctgt tctttctgaa atatggtata atagcactaa
     1561 tcaatttcta ggaaaataga tacagaaagg ggctgaaaga tgtctcatat tattgaattg
     1621 ccagagatgc tggcaaacca aatcgcggct ggagaggtca ttgaacgtcc tgccagtgtg
     1681 gtcaaagagt tggtagaaaa tgccattgac gcgggctcta gtcagattat cattgagatt
     1741 gaggaagctg gtctcaagaa ggttcaaatc acggataacg gtcatggaat tgcccacgat
     1801 gaggtggagt tggccctgcg tcgccatgcg accagtaaga taaaaaatca agcagatctc
     1861 tttcggattc ggacgcttgg ttttcgtggt gaagccttgc cttctattgc gtctgttagt
     1921 gtcttgactc tgttaacggc ggtggatggt gctagtcatg gaaccaagtt agtcgcgcgt
     1981 gggggtgaag ttgaggaagt catcccagcg actagtcctg tgggaaccaa ggtttgtgtg
     2041 gaggatctct ttttcaacac gcctgcccgt ctcaagtata tgaagagcca gcaagcggag
     2101 ttgtctcata tcattgatat tgtcaaccgt ctgggcttgg cccatcctga gatttctttt
     2161 agcttgatta gtgatggcaa ggaaatgacg cggacagcag ggactggtca attgcgccaa
     2221 gcaatcgcag ggatttacgg tttggtcagt gccaagaaga tgattgaaat tgagaactct
     2281 gacctagatt tcgaaatttc aggttttgtg tccttgcctg agttgactcg ggctaaccgc
     2341 aattatatca gcctcttcat caatggccgt tatattaaga acttcctgct caatcgtgct
     2401 attttggatg gttttggaag caagcttatg gttggacgtt ttccactggc tgtcattcac
     2461 atccatatcg acccttatct agcggatgtc aatgtgcatc caactaagca agaggtgcgg
     2521 atttccaagg aaaaagaact gatgactctg gtttcagaag ctattgcaaa tagtctcaag
     2581 gaacaaacct tgattccaga tgccttggaa aatcttgcca aatcgaccgt gcgcaatcgt
     2641 gagaaggtgg agcaaactat tctcccactc aaagaaaata cgctctacta tgagaaaact
     2701 gagccgtcaa gacctagtca aactgaagta gctgattatc aggtagaatt gactgatgaa
     2761 gggcaggatt tgaccctgtt tgccaaggaa accttggacc gattgaccaa gccagcaaaa
     2821 ctgcattttg cagagagaaa gcctgctaac tacgaccagc tagaccatcc agagttagat
     2881 cttgctagca tcgataaggc ttatgacaaa ctggagcgag aagaagcatc cagcttccca
     2941 gagttggagt ttttcggaca aatgcacggg acttatctct ttgcccaagg gcgagatgga
     3001 ctttacatca tagatcagca cgctgctcag gaacgggtca agtacgagga gtaccgtgaa
     3061 agcattggca atgttgacca gagccagcag caactcctag tgccctatat ctttgaattt
     3121 cctgcggatg atgccctgcg tctcaaggaa agaatgcctc tcttagagga agtgggcgtc
     3181 tttctagcag agtacggaga aaatcaattt attctacgtg aacatcctat ttggatggca
     3241 gaagaagaga ttgaatcagg catctatgag atgtgcgaca tgctcctttt gaccaaggaa
     3301 gtttctatca agaaataccg agcagagctg gctatcatga tgtcttgcaa gcgatctatc
     3361 aaggccaatc atcgtattga tgatcattca gctagacaac tcctctatca gctttctcaa
     3421 tgtgacaatc cctataactg tcctcacgga cgtcctgttt tggtgcattt taccaagtcg
     3481 gatatggaaa agatgttccg acgtattcag gaaaatcaca ccagtctccg tgagttgggg
     3541 aaatattaaa agtataaaaa agtctgggaa aaattttcaa aatcagaaaa acgcataaaa
     3601 tcaggtgttc aaaaaccttg attttatgcg ttttatcata gaaatattta cttcattttt
     3661 tcctaattct tttcgaaact ctttttaaac cacgtcagtt ttatcagtaa tctcaaaaca
     3721 gtgttttgag ctaattttgc cagttttgtc tgtaacatcg aagttgtgtt ttaccactct
     3781 gcgactggtt tcctagtttg ctctatgatt ttcacagagt attaaattgc gattttgcca
     3841 agtttcttta ttcgtctaaa agtagagtct gttctatgcg tctaatgtac gaattaggtt
     3901 gaccatttca atagctcctt gtgcacactc agaaccctta tttcctgctt tagtaccagc
     3961 tcgttctatg gcttgttcaa ttgtatctgt cgttaacaca ccaaacataa caggaatttc
     4021 gctatttaaa ctgatttggg cgattccctt agatacctcg ctacatacat aatcataatg
     4081 acttgtattc cctctaatga cagctcccaa gcagataatt gcatcatatt ttttactttt
     4141 tgccattttt gatgcaatca gtggtatttc aaaagctcct ggaacccagg ctacctcgat
     4201 atctttctcg tttacattct ctcttttgag attatctagt gctccagata ataattttga
     4261 agttataaat tcattaaatc tcgctacaac aatacctatt ttaatattgt ttgctactaa
     4321 attaccttca taagtgttca tttatttttc ctccatattt aaaatgtgac ccattcgatt
     4381 tttctttgtt tctaaataaa aactatcgta aggattggct tctatttcga ttgatattct
     4441 actggaaatg gtaattccat atttttctaa ctgttcaacc ttgtcaggat tatttgtcag
     4501 taaatgaagt gactgaagtc ccagatcttt aagcatttgt gctccaatat gatattctct
     4561 taaatcacct tcaaagccta atgcaagatt ggcatcaagc gtatccatgc cttgatcttg
     4621 taaatgatag gcttttaatt tattgataag tccaattcct cgtccctcct gtcgcaagta
     4681 aagtaagaca cccgaaccat tctcaacaat cattttcata gctttatcga attgctgtcc
     4741 acaatcgcaa cgtaaagagc ctaaaacatc tcctgttaaa cattcggagt ggacccgaca
     4801 taatacattg gcttcatcct ctatgtttcc cataataaga gcaagatgat gttccccatt
     4861 tagtttatct atatagctaa ttgctttgaa attaccgtat ctagtaggca tattgacagt
     4921 tgaaactcgt tctaccagct gatcatatac ttttctatat tcttgtaatt ctttgatggt
     4981 aattagtgga atgttgtgtt tcttcgagaa ctgaattaaa tcatctgttc tcatcatttt
     5041 gccatcatga ttcattattt cacaacatag gccacactct tttagtccag ctaattttaa
     5101 taaatcaaca gttgcttctg tgtgtccatt tctttctaga acaccacctt tttttgcaat
     5161 taaaggaaac atgtgtcctg gcctgcgaaa atcagagggt gttatatctt cagctacaca
     5221 catacgtgcg gtcagtcctc tttcctcggc agaaatacct gtggtcgttt ctttataatc
     5281 aattgaaact gtaaaagcag tcttatgatt atctgtattg ttttcaacca taggtgaaag
     5341 cattaattga ttagctaaac tttcgctcat aggcatacaa attaatcctt tggcataagt
     5401 agccataaaa ttaacatttt ctgttgtagc tgcttgtgca gaacaaatta agtctccttc
     5461 attttctcta tccttgtcgt ctataacaag aacaagtcgt cccttctgca atgcttctaa
     5521 tgcttcttgt atttttcgat attccattga ctgattatcc tttctgctaa aatccatttt
     5581 gatataatag ttccttagat atttctgatt ttggagagtt atccatcagt ttttgcacat
     5641 atttacctaa gatatcattt tcaagattta ctgtactccc gacttgttta ctcttaagaa
     5701 tggtttgttc caaggtatga gggataacag atactgaaaa gtttactttg gagactttag
     5761 cgacagtcag actaatgccg tcaattgtaa tagatccttt ttcaactatt aaatctaaaa
     5821 tttctttttg tgtgttgatt tgataccata cagcattatc atcttttttt attgacgaga
     5881 tttttcctgt accatcaatg tgtcctgtaa cgacgtgacc cccaagtcga ccgttgacag
     5941 ataaggctct ttctagattc acctcacttc catgttttaa tagagtaaga gctgttcgac
     6001 tccatgtttc attcattaca tcaactgtaa aggattgatg attgaaatga gtaactgtaa
     6061 gacagatacc atttactgct atactatcgc ctaaatggat atccgttaat atttttgagg
     6121 ctttaattga tagtttacaa ttacgagagt ctttctgtat tctttcaact tttccgattt
     6181 cttcaattat tcctgtgaac atggataaat cacttcactt tctatgagat agtcatttcc
     6241 tatttgagaa aaagcataag gtttcaatct aatagcgtca tttggcaaag aaatgccttc
     6301 acctccgaca ggaaacttgg cactgcctcc aaaaattttt ggtgcaatat atattttcag
     6361 ctcatcaaca atttgttgtt ccaaagcact ccaattcatt agactgcccc cttctagaac
     6421 taggctatca atctgcatgt ttcctagatg ttgcattaaa ctcgataagt ctatatgatt
     6481 gccttttttc tttatggaaa gtatttcaca gccatgattt tgatatagct tcattttatt
     6541 tttgtcttca gaggaagtgg caatgtaagt tttaatatca tttgctgttt ttacgatttt
     6601 agaggtaaga ggagttcgta aatgtgtatc gcatatgata cggataggat ttttcccttc
     6661 ctccaatcta catgtcagca aaggatcgtc ttgaataaca gtattgactc ccaccataat
     6721 tgcactaaca tagtgtcgta actgatgcac atgctttctt gcttcttctt cagtaatcca
     6781 tttggattga tttgttttag tggctatttt tccatccatt gacattgcat atttcataaa
     6841 aacatagggt acatgctggg taatatactt tctaaaactt tttattaagt taagacactc
     6901 attttctaaa attccaacag taacttgaag attattttcc tcaagtatct ttactccttt
     6961 tccagataca ataggattac agtctaggct tccaatgact actcttgtaa taccactatc
     7021 gattatagca tctatacagg gaggtgtttt cccgaagtga caacagggtt caagtgttac
     7081 ataaagcgtc gctccgacag gggattttct acagttttta agagcatttc tctcagcatg
     7141 tgggccacca aaaaactcat gataaccttg tccgataatg tgattatctt ttacaataac
     7201 tgcgccgacc ataggattgg gattgacgta accagcccct ttttgtgcca gttttattgc
     7261 taatttcata tattttgaat cgctcatctc actacctcca aaaaaatata ccttgaatag
     7321 gggactactc aaggcataca aaagaaaact tatgcgatta acaaaaatgc tctgaaatga
     7381 caagtaatca tttcagagca cgcaaaaagc acaaatatac ttttatcttc tttcatccag
     7441 actatactgt cggctttgga atttcaccaa atcatgcctt tcggctcgtg ggctatacca
     7501 ccggtaggga attgcaccct gccctgaaga tagttattca attacagatg attatagtac
     7561 ttaattttga atatgtcaac agataaatac cgattgtttt tgatatactg tatttgtgat
     7621 aatcgattct cgctcctcgg ataaagaaaa tatgatatac tagataaacg aaataagaga
     7681 gaaggaacac tatgtacgca tatttaaaag gaatcattac caaaattact gccaaataca
     7741 ttgttcttga aaccaatggt attggttata tcctgcatgt ggccaatcct tatgcctatt
     7801 caggtcaggt taatcaggag gctcagattt atgtgcatca ggttgtgcgt gaggacgccc
     7861 atttgcttta tggatttcgc tcagaggatg agaaaaagct ctttcttagt ctgatttcgg
     7921 tctctgggat tggtcctgta tcagctcttg ctattatcgc tgctgatgac aatgctggct
     7981 tggttcaagc cattgaaacc aagaacatca cctacttgac caagttccct aaaattggca
     8041 agaaaacagc ccagcagatg gtgctggact tggaaggcaa ggtagtagtt gcaggagatg
     8101 accttcctgc caaggtcgca gtgcaagcaa gtgctgaaaa ccaagaattg gaagaagcta
     8161 tggaagccat gttggctctg ggctacaagg caacagagct caagaaaatc aagaaattct
     8221 ttgaaggaac gacagataca gctgagaact atatcaagtc ggcccttaaa atgttggtca
     8281 aataggagca gagaatgaca aaacgttgtt cgtgggtcaa gatgaccaac ccgctctaca
     8341 tcacctatca tgatgaggag tggggccagc ccctccatga tgaccaagca ttgtttgagt
     8401 tgttgtgtat ggaaacctat caggcaggcc tgtcttggga aacggtactc aacaaacgcc
     8461 aagctttccg agaagccttt catagctatc aaattcactc agtcgcagag atgactgaca
     8521 ctgaattgga agccatgctg gagaatccag ctatcattcg aaatagagcc aagatttttg
     8581 ctacacgcgc taacgcccaa gcctttctac agttacaggc agagtacggc tcttttgatg
     8641 cctatctttg gtcttttgtt gaggggaaaa ctgtcgttaa cgatgttcct gattatcgcc
     8701 aagccccagc taaaacaccc ttatctgaga aattagccaa agatctcaaa aaacgaggct
     8761 tcaagttcac aggcccagtc gccgtattgt cttttctaca ggctgcaggg ctagttgatg
     8821 accacgagaa tgattgtgag tggaaaggtc ttaaatgatg tctaacaaaa ataagggaat
     8881 tctgattttt gcgattctct atacagtcct ctttgtgttt gatggcgtta aattgctggc
     8941 ttctttaatg ccatctgcca ttgcaaatta tcttgtttat gtagttttag ctttatatgg
     9001 ctccttcttg ttcaaggata gattgatcca acaatggaag gagattagaa agactaaaag
     9061 aaaattcttc tttggagtct taacaggatg gctctttctc attctgatga ctgttgtctt
     9121 tgaatttgta tcagagatgt tgaagcagtt tgtgggacta gatggacaag gtctaaatca
     9181 gtctaatatt caaagtacct ttcaagaaca accactactg atagctgttt ttgcttgtgt
     9241 cattggacct ctggtagaag aattattttt ccgtcaggtc ttattgcatt acttgcagga
     9301 acggttgtca ggtttactaa gcattattct ggtaggactt gtttttgctc tgattcatat
     9361 gcacagtttg gctctatcag agtggattgg tgcagttggt tacttaggtg gaggccttgc
     9421 cttttctatt atttatgtga aagaaaaaga gaatatctac tatcccctac ttgttcacat
     9481 gttaagcaac agcctctcct taatcatttt agctatcagt atagtaaaat gaaatgagaa
     9541 caggacaaat cgatttctaa caatgtttta gaagtagagg tgtactattc tagtttcaat
     9601 atactgtaat atgtgatgaa aatgccagta atgataccga gaaaaaagct gagaaacttt
     9661 tcccagcttt atttgttata gtcaaagaga atgacttgtt cctgtgcatc tacatgagca
     9721 tggaccccaa agggtacaat tgctcttgga gttgcgtggc cgacattcag attatagaca
     9781 atcgggatat tgctgtcaat gatatccaat agtgcctctt tatagtcgtc atggaaagtt
     9841 tcatccatag gttttccgac caagagtcca ttgatgaccg cgaatatgcc agtgtccttt
     9901 aaagttagca acatcttttt gaagtcttct ggcttaggct tttcttcgct tgtttcgagc
     9961 aagaggattt tcccttccca gtctgacaag tcagggaaaa gtttgtattt ttggcagagt
    10021 tccgtgctat ctgcgtatcg agagttgtca aagatatcgt agagggattc gaggcaacca
    10081 ccgaggattt tcccctcgaa ctgggcactt ccttgcaaca agtcaaaacc tgtatttgta
    10141 tgactgacac gaggtgttcc cagggccgtg ggactaaaat cagttcgttc ctcataccaa
    10201 acgtcactag ggcggatttc tgaaattctt cccgtctcaa tcaattcttt aaagtagtga
    10261 aggctatagg ctagcatttc tttgtctaat tcacaaatgt ctgctaaaaa ggattgacca
    10321 taaaaagtct tgattcctaa tttatgcaac atgaggtggt tcatggttgt atccgagaag
    10381 ccaagaaaaa ttttttgttt gatacccttt tggagttggt cattttcaaa aagataaggt
    10441 agcaagcgat aggcatcgtc tccaccgatg gcacatagga tcatgtcgat ctatcatcag
    10501 aaaaggcatg aatcaaatcc tctgcacgag cttcaggatg gtccttgata aagtctaatc
    10561 cttttaacga atggggcaaa aagatgggat tgagtcccag atccttgaga cgttggatac
    10621 ccaagtccac ttcgtgtttg acaaaatcct ctccgataat gccactagat aaactaacaa
    10681 taccaatagt agaaaccata tctcatcctc ctagaaatag attgagccta ttttatcaca
    10741 aaatataaga gaaagctatg tgggatttca gaagaatact tttaatactc ttcgaaaatc
    10801 tcttcaaacc acgtcagttt tatttgcaac ctcaaagctg tgctttgagc aacctgcagc
    10861 tagcttccta gtttgctctt tgattttcat tgagtataac tcaaaaaagt aagtgaaaaa
    10921 gcaaccgcac ctgcagttgc ttttatcgtt ttcttattct tgatagcgtg ttggtcctgc
    10981 acttgcacta cggatattca agcgtttctt gagatagaag cgaagggcta ggagggctgc
    11041 tccaataata gctagtggca atggatccag tactgggtta aggctagctg gtaggaagct
    11101 tgttgcaaag aagacaagca accaaaggaa catagaagct aggataacta gtacagattt
    11161 ccagaaaggt ggacgttgac tgcgatccat atctggtcca tagtattggt aaacaaagta
    11221 gtacatcaag tagaaggcaa atccaccaac cagtccaact aatagaagag taatcaatcc
    11281 atagccgaaa gcttgatctg ccgcaaagaa ggttgtgagg gcgctgacaa gggcaaagag
    11341 gctagtgatg aaaagagctg agtccataat catcagtttt ggatcatcat tttcttttgg
    11401 atgctctttt tcgtactgct ctttgacagt gaagctatga gcccaatgag ttggtgcgcc
    11461 gtatagggaa cgggcagttg tacctttaga ttgctcctca aggatttggg gaatgacttc
    11521 ctcaaaaata gtctggattt cagcgtctgt tttcccatct ttgatgaatt gttgggtagc
    11581 aatgtggaca aactcttggt ttttcttagt taatttttgt agatcaattt gagacatagg
    11641 aactcctctt agaaccattt tttatggatg agatagagag tgagcgagac actcatagca
    11701 aaggcgataa agacgattaa ccagaaggca tttggctctc cgtttagggg gatttcatta
    11761 tccttaaagt tcatcccgta ggcagaaaag accatggttg ggatggacat gacgatggtc
    11821 acaagggcca aggttttcat gatgttgttc tggttgttag aaatgataga ggcaaaggtc
    11881 tctgtcatag aatgcaagac gtttccataa atatctgcca tctcgatggc ctgttgggtt
    11941 tcaatcaggg tgtcttcaag caggtcttcg tcttcaaggt atttcttgat attgctggtt
    12001 gaactggtca atttcttaat cacgcgctca tttgttttga gggaggcctt gaaatagacg
    12061 atagtttttt ccaattccat gagctcaatc aattcttcat tacgagttga ttgatgcagt
    12121 tgactttcga tttgttcact cttgcggtcg attgaacgaa gggctgttag gtaaagctct
    12181 gcattgcgat aaagaatttg aaagataaaa cgtgaacgca tgaaggtata gaaattacgc
    12241 aatcgacggt tgataaagac atcaaggaca ggtagtggtt ccaaacacgt agtgataatg
    12301 gtttcctcag tgatgataat accaagcggg atggttacgt agtaggtgcg gttatttctt
    12361 tcctccgtga ccggcacgtc tacgataatc agggtatact cgtcttcaat ggtaatacga
    12421 gacatttctt ccgcatcgag cggtgctcga aggtcggcaa tatcaatatc gaaggtgttg
    12481 gcgatttcga gtgattcatt ttgagtcgga ttgacgagat tgatccaagt acccggttca
    12541 agcgtatcga tctctttaaa ttcagttgtt gtagagagaa aaacttgttt catatccctt
    12601 atcctttctc atttttcaga ttttttcaca ctgtactatt atactacaaa atcggccttt
    12661 tgggtaatag aatctcactt tttttggtat aatggtaagc aataatggac tagaaagaac
    12721 aaagatgcaa gataaaattg tcattcatgg ggcgcgtgcc cataatttaa aaaatattga
    12781 tgtggagatt ccgcgagaca agttggttgt cgtgactggc ttgtcaggtt cagggaagtc
    12841 cagtctggct tttgataccc tctatgcgga gggacaacgt cgctatgtag agagtttgtc
    12901 cgcctacgct cgtcagttct tgggaaatat ggaaaaacca gatgtagatg ccattgatgg
    12961 tctcagtcca gctatttcta tcgaccagaa aacgactagc aaaaaccctc gctcgacggt
    13021 gggaacaacg actgaaatca atgactatct gcgtctcctc tacgcacgtg tggggacgcc
    13081 ttactgtatc aacggacatg gagctatcaa tgcttcttct gtggagcaaa tcgttgataa
    13141 ggttttggag ttacctgaac gccagcgctt gcagatcttg gcctctgtca tccgcaagaa
    13201 aaaaggccaa cataagagtg ttatcgagaa ggttcagaaa gacgggtatg ttcgtgtccg
    13261 tgtggatggg gaagtctatg atgtgaccga agtgccagag ttgtctaaga gcaagcaaca
    13321 caatatcgat gtcgtggttg accgtattgt tatcaaggag ggcattcgta gtcgtctctt
    13381 tgattccatt gaggctgccc ttcgtattgc agaaggttat gtcattatcg acaccatgga
    13441 cgactctgag ttgttgttct ctgagcatta tgcctgtcca gtttgtggtt ttactgtccc
    13501 agagttagag cctcgtctct tctctttcaa tgctcctttt ggctcttgta gtgagtgtga
    13561 cggcttgggc atcaagctgg aggtggatac tgatttggta gtgccagatg ccagcaaaac
    13621 cttacgtgag ggagcgttag caccttggaa tcctatctca tccaactact atccaaacat
    13681 gttagagcag gccatgaaag tatttggagt ggctatggat aagccttttg aggacctgtc
    13741 agaagaagat aagaacttga ttctctatgg gtcagatggt aaggaattcc atttccacta
    13801 tgagaatgaa tttggtggcg tgcgcgatat cgacattccg tttgagggag ttatcaataa
    13861 tatcaaacgt cgttaccatg aaaccaatag cgattacact cgcactcaga tgcgtctcta
    13921 catgaatgag ctgacctgcg gaacctgtca aggctatcgt ctcaatgacc aggccttgtc
    13981 tgtccgtgtg ggcggccagc aagggccaca tattggagaa atctcagacc tgtctatcgc
    14041 tgaccacttg gacttggtga gccagttaac tttgtctgaa aatgaagcca tcattgctcg
    14101 tcccattctc aaggaaatca aggatcgttt gaccttcctt aataatgtgg gtcttaacta
    14161 tctaaccctg tcacgttcag caggaaccct ttcaggtggg gaaagtcagc gcattcgttt
    14221 ggcaacccag attggttcca acctatcagg cgtcctctat atcctagacg agccgtcaat
    14281 cggtcttcac cagagagaca atgaccgcct gattgccagt ctgaaaaaga tgcgtgactt
    14341 gggcaatact cttatcgtgg tggaacacga cgaagatacc atgcgtgaag ctgattatct
    14401 gattgacgtt ggtcctggtg ccggtgtttt tggtggtgag attgttgcag caggtacacc
    14461 taagcaagtg gctcgcaaca gcaagtctat cacaggtcag tacttgtcag gcaaacgtgt
    14521 catcccagta ccagaagagc gccgtgtcgg aaatggtcgt tttattgaag tgataggagc
    14581 gcgtgagaac aacttgcaaa atgtcacggc tcgctttcca ctaggaaaat ttatcgcagt
    14641 gacaggtgtg tcgggttcag ggaaatcgac tctaatcaac agcattctca aaaaagccat
    14701 tgcccagaag ctcaaccgta attcagacaa acctggtaag tttaagacta ttacagggat
    14761 tgagcatgtc gaccgtttga ttgatattga ccagagccct atcggacgga cgccgaggtc
    14821 taacccagct acctatacag gagtttttga cgatatacgt gacctttttg ctcagacaaa
    14881 tgaagccaag attcgaggct acaagaaggg gcgcttcagt ttcaacgtca agggaggtcg
    14941 ctgtgaagct tgctcaggtg acgggattat caagattgag atgcacttct tgccagatgt
    15001 ttatgtggct tgtgaagttt gccacggaac ccgctataac agtgaaaccc tagaagttca
    15061 ctacaaggaa aagaatattt cgcaggtctt ggatatgacg gtcaacgatg cggtagaatt
    15121 tttccaacac attccgaaaa ttcaacgcaa actccagacc atcaaggatg tagggctagg
    15181 ctatgtgacc ttgggacagc cagctaccac cctttctggg ggagaagccc aacgtatgaa
    15241 gttagctagc gaactccaca aacgctcgac aggaaaatct ttctacattc tggatgagcc
    15301 gacgacaggg cttcatacag aggacattgc tcgcctgctc aaggttttag ctcgctttgt
    15361 agacgatggc aatacagtcc tcgtcatcga gcacaatctg gatgttatca agacggcaga
    15421 ccatatcatt gacttgggac ctgagggcgg tgtcggtggt ggaaccatca tcgtaacagg
    15481 aactccagaa gaagtagctg ccaatgaagc cagctataca ggacactatt tgaaaggaaa
    15541 gttacatcat gaataaacgc gtacaagcat ttctagctaa aatgcaagaa aaagaactag
    15601 atggtatcat catcaacaat cttaaaaacg tctattattt gactggtttt tggggctcaa
    15661 acggaacagt ctttatcagc cgtgaccgtc aggtcttagt gacagactct cgctatatca
    15721 tcgcagctaa gcaagaaacc agtggttttg agattgtggc tgatcgtgat gaattggctg
    15781 tcattgcagg aattgttaag gacatgggct tgactcgtat cggttttgaa gatgagattt
    15841 cagtatctta ttaccaccgt atgcaggcag cttttgcagg tttggacttg cttccacaaa
    15901 ctcaatttgt ggaaggtctt cgtatgatta aggatgaggc agagattgca gcgattcgca
    15961 aggcttgttc tatctcagac caagctttcc gcgatgcgct tgactttatc aaaccaggaa
    16021 aaactgaaat tgaaattgcc aacttccttg atttccgcat gcgtgagttg ggagcatcta
    16081 gcttatcttt tgatacgatc ctagctagcg gtatcaattc ttctaaaccc catgcccatc
    16141 caatgcacaa accagtggag ttgggagaag ccattaccat ggacttcggc tgtctctatg
    16201 accactatgt cagtgatatg acccggacta tctatctagg gcatgttagc gatgagcagg
    16261 cagagattta caatacggtt ctaaaagcta accaagcctt gattgaccaa gctaaggcag
    16321 gcttaggttt ccgtgacttt gacaaaatcc ctcgtgatat tatcattgag gcaggttatg
    16381 gcgactactt tactcacggc attggccacg gtattggtct ggatatccat gaggaaccct
    16441 actttagcca gacttctaca gaaactatta agacaggtat ggccttgacc gatgaaccag
    16501 gtatctatat cgaaggcaaa tatggcgttc gtatcgagga tgatatcctg attacagaga
    16561 caggttgtga attattgacc ctagctccaa aagagttgat agtcatttag ttgtttgtca
    16621 agaaaaaagt acgaaaatga cgaaaaaagt caaaaaaaat taaaaatagg tcgcaagtcg
    16681 gatgtttttt atggtataat agactaaact gatagtaaca tgtagcgaaa ggggtaggta
    16741 catgattaaa atttatacag tctcaagttg tactagctgt aaaaaagcaa aaacctggct
    16801 caatgcccac cagttaagtt ataaagaaca aaaccttggt aaagaaggaa ttacgagaga
    16861 agaattactg gatattctaa ccaaaacaga taacggaata gccagcatcg tttcgtctaa
    16921 aaatcgctat gccaaagccc ttggagtgga tattgaagat ttgagtgtca atgaagttct
    16981 caatctgatt atggaaacac cgagaatttt aaagagccca atccttgtag atgaaaaacg
    17041 cctgcaagtt ggctacaagg aagacgatat tcgtgccttc ctaccacgct ctgtccgtaa
    17101 tgtagaaaat gcagaagcac gtttacgtgc agctctataa acatcaaggc tgggagcact
    17161 tcccagtctt attctatttt aatttcaaaa agaaagaaga gagaaatgaa aaaaatagtt
    17221 cttgttagtc tagctttcct ttttgtcctg gttggttgcg gacagaaaaa agaaactgga
    17281 acagctacaa aaacagaaaa ggatacgctt cagtcggcat tgccagttat tgaaaatgcc
    17341 gagaagaata cagttgtaac caagactttg gtcttgccca agtcagatga tggtagccag
    17401 caaactcaaa ccattacata caaggacaag acttttttga gcttaacgat tcaacaaaaa
    17461 cgtccagttt ctgatgagtt gaagacttat atcgaccaac atggagtgga agaaactcaa
    17521 aaagctcttc ttgaagcgga ggagaaggat aagtctatca ttgaagctcg taaattggca
    17581 ggtttcaaac ttgaaacaaa actattgagc gcaacggaac ttcaaacaac gactagtttt
    17641 gattttcaag ttctggatgt caagaaggct tcccagttgg aacatctgaa gaatattggt
    17701 ttggaaaatc ttttgaaaaa tgaaccaagc aaatatattt cagatagatt ggcaaatggc
    17761 gcgacagaac aatagaaaat ccaaataaat agactgcctg aattgtagaa ggtaaggaat
    17821 tatgctataa tggtactatt ctaaggaaag agggtgttag aatgggattt actgaagaaa
    17881 cagtacgttt taaattggac gattccaata aaaaagaaat tagcgaaact ttgacagatg
    17941 tttatgcttc gttgaacgat aagggttaca acccaattaa ccaaatcgta ggttacgtat
    18001 tgagtggaga ccctgcctac gttcctcgtt ataataatgc acgaaatcaa atccgtaagt
    18061 atgagcgtga tgaaatcgtt gaggaattgg ttcgttacta cctcaaagga caaggagtcg
    18121 atctataacc tatgagaatt atgggattgg acgtcggttc aaaaacggta ggggtggcga
    18181 ttagcgatcc gcttggtttt acagctcaag ggcttgaaat catccagata aatgaagaac
    18241 aaggccaatt tggttttgac cgcgttaagg aattggttga tacttacaag gtagaacgat
    18301 ttgtagtggg cttgcctaaa aacatgaaca atacaagtgg accgcgcgta gaagctagtc
    18361 aagcctacgg agcaaagcta gaagagtttt ttggtttacc agtagactat caggatgaac
    18421 gcttgacaac agtggctgct gagcgcatgt tgattgaaca agcagatatc agtcgcaata
    18481 agcgcaagaa agtcattgat aagttagcag ctcagctgat tttacaaaat tatttagata
    18541 gaaaatttta atataaagga gaggctatgt cacacgatca taaccacgac cacgaagaac
    18601 gtgaactaat cacactagta gatgagcaag gaaatgaaac cttgtttgaa atccttttga
    18661 caattgatgg aaaagaagaa tttggtaaaa actatgttct tctagtacca gttaacgcag
    18721 aagaagacga agacggacaa gttgaaatcc aagcttactc attcattgaa aatgaagatg
    18781 gaacagaagg cgaattgcaa ccaatcccag aagactcaga agacgaatgg aacatgattg
    18841 aagaagtctt caacagcttt atggaggagt gatacagtct gggagactgt atcagcccaa
    18901 ctcccagaaa tggggaagag ttggaacgtc cagtgaacat ttttacccca actcttagaa
    18961 cttgagaaga gttggaacat ctagtggatg tttttagccc atattcacat tcatagtagc
    19021 tagttattag ctaaccaggt aagaggtcgg gacgaaaatc ctggccttgt ttttattagt
    19081 aaccatactt taatgcgata attgattgga cttttgtcaa ggagatgtgt atgtttgaag
    19141 tagaagaatg gctccatagt cggattggtt tgaattttcg atcaggtttg ggccgaatgc
    19201 agcaagcggt ggatttgtta ggaaatcctg agcagtctta ccctattatc cacgtaacag
    19261 ggactaatgg gaaaggatct accattgctt ttatgaggga attatttatg gggcatggca
    19321 aaaaagttgc gacctttacc tcccctcata tcgtctctat caatgaccga atctgcatta
    19381 atgggcaacc tatagcagac gcagacttta tccgtttgac tgatcaggtc aaagagatgg
    19441 agaaaacgct tctgcaaact cctgcccagt tgtccttttt tgaattgctg accttagttg
    19501 cttttcttta ttttagggag caggaggtgg atttggtttt attagaagtg ggaattggtg
    19561 gcttacttga cacgaccaat gtggtaactg gagagcttgc tgtcatcacc tccattgggc
    19621 ttgaccatca agaaaccttg ggtgatagtc tagaagcaat tgcagagcag aaagctggta
    19681 ttttcaagga tggtaagaag gcagtgattg cgaaattgcc tccagaagct aggcttgtct
    19741 gtcagaaaaa agccgaatct ttagctgtta acctttatca ggcaggtcaa gattttttaa
    19801 tgctgaatgg tgatttttca agctctttac taaatctttc acagctgaac ataggcttag
    19861 aaggagtcta tcagcaggag aatgcagcct tggcgttgca aacttttctt ctttttatga
    19921 gagaaggaaa ggaagctgtt gatgaacagg ctgtaagaaa ggccttggaa cagacccatt
    19981 gggctggtcg cttggagcgt attcgcccac agatttactt ggatggtgct cataacctcc
    20041 ctgccttgac tcgcttggtt gagtttatca aagaaaaaga gcaggaaggc tatcgacctc
    20101 aaatcctctt tggatccttg aaacggaagg attatcaagg gatgttgggt tatctgactg
    20161 aaaatttacc tcaagtggaa ctcaaaatga ccggctttga ctatcaaggg tctttggacg
    20221 agacagatgt cacaggttac catgtaattc cctcttaccg agaatttatc agcagtttcg
    20281 aagaaagggc agacgctcaa gacttgctgt tcgttacagg gtctctctat tttatctcag
    20341 aagtacgggg ctacctgctg gaccgtgagc agataaattg acccccttgt ttgaacttgt
    20401 tatactaggg gaattgctgg ttagagaaaa tttctctaaa ttggtagcag aaaggaaatt
    20461 catcatgaaa ttaaaaagat tcacactttc tcttgcttct ctagcaagtt ttagtctctt
    20521 agtagcttgt tcacaaagag ctcaacaggt tcaacagcct gttgctcagc aggtccaaca
    20581 gcctgctcaa cagaatacca atactgcaaa tgcaggaggt aaccaaaatc aagcggctcc
    20641 agtacaaaac caacctgttg ctcaaccgac cgatattgat gggacttata ctggtcagga
    20701 tgacggagac cgtatcactt tagtggtaac tggaacgact ggtacatgga ctgagctcga
    20761 atctgacggg gatcagaaag tcaaacaggt tacatttgat tcagcaaatc aacgcatgat
    20821 tattggcgat gatgtcaaaa tttacactgt aaacggtaat caaatcgtcg tagatgatat
    20881 ggatagagac ccatcggacc aaatcgtttt aactaaataa gactatgcaa gtagaaaggg
    20941 ctggattttt cagccttttt acttttacct agaaaataat cataaatact tgccttctac
    21001 cgaatacctg cgttatacta gtatcaatta cttgttttta gcattcaaac tatcaaggag
    21061 gggatatgaa atataggaaa tttcaattat tgatgtccaa gtatggcttt agtctttcga
    21121 ttatgctact tgaactttgt cttgtttttg gtctctttct ttatttagga cgcatggctc
    21181 ccattttatg gattactgtc ctcattctac tgagtatcat cacaatcatt tcgatagtca
    21241 accgtaatac gactcctgag aataaggtaa cctggttgtt agtagccttt gtgccagtat
    21301 ttggtccctt gctctatctg atgtttggtg aaaggcgatt gtccaaaaaa gaaatcaaac
    21361 aactgaagaa gctaggctct atgcatttcc aagaagcaaa tagccagcta ctaaaagaga
    21421 aattaaaaga aagtgacaag gcagcttatg gagtcatcaa gtccttattg agtatggata
    21481 ccaatgctga catctatgat caaactgcct ctacattttt tcctaacgga gaagctatgt
    21541 ggaaaaagat ggtagaagat cttaaaaagg ctgagaaatt tattttcttg gaatattaca
    21601 ttatagaaga aggtttgatg tggaatcgca tactagatat actagagcaa aaggtagctc
    21661 agggtgtaga ggttaagatg ctctatgatg atatcggctg tatggctact ttaacaggag
    21721 attatgcaca tcgacttcgt cagctgggca tcgaggccca taaattcaat aaagttattc
    21781 ctcgtttgac agtggcttat aataacagag atcatagaaa aatattgatt gttgatggtc
    21841 agatagccta tactggtggg gtcaatctgg cagatgagta cattaaccac gtcgagagat
    21901 ttggttattg gaaggatagt ggaattcgct tagacggact agcagtaaaa gccctgacac
    21961 gcttattttt gaccacttgg tacattaatc gaggagaaat tagtgatttt gatcaatatc
    22021 atttagaaaa tcattctatc ccgagtgacg gtttaaccat tccatacgga agtggaccca
    22081 agccaatttt tcgagcgcag gtagggaaaa aagtttatca gagtttaatc aatcaagcaa
    22141 cggaatcggt ctatattacg acaccttatt tgattataga ttatgattta acagagacaa
    22201 tcaaaaatgc agctatgaga ggggtcgatg ttcgaattat caccccttac ataccagata
    22261 agaagttcat tcagttagtc acgagagggg cttatcccga ccttctttct gctggtgttc
    22321 ggatttatga gtatagtcca ggttttattc atagtaagca gatgttggta gacgaagatt
    22381 ttgcggtggt ggggacaatc aatctcgact accggagctt ggtacaccat tatgaaaatg
    22441 cagtcttact ctataaaact ccttctataa tggaaatcgc ccgagatttt cgaaatatat
    22501 ttgcagattc tcaggaagtc tatcctcatt ctatcaaaac gagctggtat caaaagcttg
    22561 taaaagaaat cgcccagcta ttcgccccta tcttataaga aatctagaac ttaggataca
    22621 aaccagacct atttttcttg tcttttacga atagaaagaa agaggagggg aattcatgct
    22681 aactaaggaa gaagtgaatg atatgattga gttcaaacag acacatttac accgctttcg
    22741 ggaaattgaa acctttgtga aactatgcaa aaaatcactc aaacagccat cgcaagctga
    22801 caatcctagg actttttgat atactaagac agataaattg aacaggagaa acgatatgag
    22861 tgtgtatggt agagtagaag aagttcataa ggagaatcgt gaacctctag aataccaaat
    22921 cgaacaagaa tcgcatcatc gtgaatcaag tcgtcttcct ttggtgaaaa ttttactatg
    22981 gagtacgctt gtaacgggga taactctagg agtaccgcta ttactcgact taatgagtgc
    23041 acaagaagtg caggattttt atgcaggttg ggcgcttcat cagacaggga agatttacag
    23101 cgactattat ggaagtcaag gtttgcttta ttatttgctg acttacgtga gtcagggcgg
    23161 atatttcttt gccatctttg agtggttagc cttggtagca ggaggatttt tcctttttag
    23221 atcagcggac accttgacag agcaaggaga ccaagctgga catctggtga ctatttttta
    23281 catgctagtt acaggtcttg cttttggtgg aggctatgcg actcttttag cgcttccttt
    23341 cttattcgca gcctttagtt tagttgcggc ttacctaagc aatccaagcc atgataaggg
    23401 atttgtacgg attgggctag ctttggcagg cggatttttc tttgctccct tatcatcgct
    23461 cctgtttatt gctgtagtga gtttaggctt gttggtcttt aaccttgggc atagacgttt
    23521 tgtgcatggg ttttatcagt ttcttgcagt ggctttaggt ttttcacttg tcttttatcc
    23581 aactgcctac tatagtgctg caacaggaag ttttggggat gcgattagtg gtattcgtta
    23641 tcctattgac agtattcgct ttgattttac ttctaaaatt ttagagaata tgttttttta
    23701 tggcttgttg tcccttggtt tgggatttgt ggtttgtatc tttttaggtc tctttcaatc
    23761 caaacccttt aaactatacg tcatttcggt tcctgcaagt cttgtggtaa ttttaggttt
    23821 gattttactt ttcttttcac aagagcctct gcacgcttct tatttgatgg tcgtcttccc
    23881 tgttttccta cttttattgg taaccaatat taagagtcaa cagagggggc gtagtgctag
    23941 aagaagccga agagaaacgc cagttagcct atggagtcgt ttcttcaaag gaaatctata
    24001 tctgctagtt tttgggtttg tctatctttt gtctgttcct tttttgatga agtttgtcct
    24061 ttatccagta ccttatcaag aacgtaatcg tcttgctgat ttggtaaaag aggagacaaa
    24121 tacggaagat gctatctatg catgggatga tactgcgact ctttatcgta agagtgagcg
    24181 cttgtcgcca tcggcgattt tgtacccgtt gcactataca gcaactgaag aaaatcgtaa
    24241 taagttactt aatgacttga aagaaaaaca acctaaggtg attgtggtaa atgacaaggt
    24301 ggtagtctgg tctgaagtgg aaacactctt aaaagaaaat taccaacaag taaagactga
    24361 ttactcagag tttaaagtct ataaaattaa ataaccaaat caatatcttg tgtattttta
    24421 aaaattttag gatttttaac acaagatatt gatttttctt tttagagtgg tataatactt
    24481 tttagaaaga acattttaga aaagagcatg catatgattg cactagaaga aaaaattaca
    24541 attttgccaa ctctcttcgt cgagaaacga gatgggagac gtgttgtatt tgatgtggac
    24601 aagattgaca aggctctcca caaggcggct gacaaggtta tggatgtgac acccctggtt
    24661 gaaaaacgcc tcaatgatct gactgagcga attattacag aaattcatag tcgctttcca
    24721 cagggaatta agatttacga aattcaaaat atcgtagaac atgaactcct tgaagccaaa
    24781 gaatatgcac tggctgagga gtatattact tatcggacac agagggattt tgagcgctca
    24841 aaagcgacgg atatcaactt tagtattcat aaacttctca acaaagacca gacagttgtc
    24901 aatgaaaatg ctaataaaga cagtgatgtc tttaacactc agcgtgattt gacagcaggg
    24961 attgttggga aatcaatcgg actgcaaatg cttcctaagc acgtagccaa tgcccaccaa
    25021 aagggggata tccactatca cgatttggac tacagtccct atacccctat gaccaactgc
    25081 tgtttgattg attttaaggg tatgttggaa aatggtttta agattggaaa tgcagaggta
    25141 gagagtccca agtctatcca gactgcgaca gcacagattt ctcaaatcat tgccaacgtt
    25201 gcttctagcc agtacggtgg ctgttcagct gaccgtatcg atgaaatttt ggcgccttat
    25261 gcagagaaga actatcaaaa acatctcaaa gatgcagaag agtgggtatt gcctgaaaaa
    25321 cgggaagatt acgcttggaa gaaaacgcaa aaggacatct acgatgccat gcaatctctt
    25381 gagtatgaaa tcaatactct cttcacttca aatggacaaa caccttttac ttcgctaggt
    25441 tttggtctgg gaaccagtcg ttttgaacga gaaattcaaa aagctatttt aaacattcgc
    25501 atcaagggtc ttggttcaga acaccgtacg gctatctttc ctaaacttat tttcactctg
    25561 aaaagaggcc ttaatttaga ggaaggaact cccaactacg acatcaagca gttggctctt
    25621 gagtgtgcaa ccaaacggat gtatccagat gttttatctt acgataagat tgttgacttg
    25681 acaggatcct tcaaggtgcc tatgggttgc cgttctttcc tccaaggatg gaaggatgaa
    25741 aatggtgtag aagtcaattc aggtcgcatg aatctgggtg ttgtgacggt taatctgcct
    25801 cgtattgctc ttgagtctga aggtgatatg aataagttct gggaaatctt caacgagcga
    25861 atgaatatcg cagaagatgc tcttgtttac cgtgtcgaac gcactaaaga ggcgacacca
    25921 gcgaatgctc ctatccttta tcagtacggt gcttttggcc atcgtctagg taaagaagaa
    25981 agtgttgacc agctctttaa gaatcgtcgt gcgaccgttt cgctgggcta tatcggcttg
    26041 tatgaagtag cgacagtttt ctttggtaac agctgggaaa gtaatccaga tgctaaggaa
    26101 ttcacgctag acatcattca cgatatgaaa cgccgtgtag aagagtggtc agaccaatat
    26161 ggctaccatt tctctatcta ctcaacacca tccgaaagtc tgacagaccg tttctgtcga
    26221 ctagatatag acaagtttgg ctctattcct gatatcacag acaaggaata ctacaccaac
    26281 tctttccact acgatgttcg taaaaatcca acaccgtttg aaaaattgga ctttgagaaa
    26341 gtctatccag aagcaggtgc gtcaggtggt ttcatccatt attgtgagta tccagtcctt
    26401 cagcaaaatc caaaggcctt ggaagctgtc tgggattatg cttatgaccg tgtaggctat
    26461 ctaggcacca atactccgat tgaccgttgc tacaagtgtg actttgaagg ggattttgaa
    26521 ccaactgaga gagggtttgc ttgtccaaac tgtggcaata gcgaccctaa aacagtagat
    26581 gtggtgaagc gaacttgtgg ctacctaggt aatcctcaag caagaccgat ggtcaacggg
    26641 cgtcacaagg aaatcgctgc gcgtgtcaaa catatgaatg gttcaacgat taaaatagct
    26701 gggcatcaag taacagatta gaaagaaatg aaatgggaaa atatcaacta gacgataagg
    26761 ggtgcgcaca agtgacccgt tatcacgaga aacactctaa aggtggagct ggtaagaaag
    26821 aacgcttgct tagcttcaga gaacaatttt taaacaagaa caagaaaaaa taaaagtgag
    26881 agccagctct cgcttttctc atagtgggag gtaaggatgg aattacgcag accaagatta
    26941 gcggataaga aagctgtttt agatatgatg acagagtttg aaaaatttca gtcgcctcac
    27001 gacggcggtt tctgggatac agagaacttt gtgtatgaag actggttaga aagcaatcag
    27061 gaacaggaaa tggggattaa tctgcctgaa ggatgggttc ctgcaattca gttagtggct
    27121 ttttctgaga aaggtcaagc agttggattt cttaatctcc ggttgcgcct cagtaacttt
    27181 ctactagaag aaggtggcca cattggctac tccattcgtc catctgaaag aggcaagggt
    27241 tatgcaaaag agactctccg tcagggcttg caagttgcta aggaaaagaa catcaagaaa
    27301 gctctggtga cctgtagcgt gaataatcct gctagcagag cagtcattct agcaaatggt
    27361 ggaatatttg aggatgctcg caatggagtc gagcgttatt ggatagaggt agcgaatgaa
    27421 taatccaaaa ccacaagaat ggaaaagcga ggaacttagt caaggtcgta tcattgacta
    27481 caaggccttt aactttgtgg acggcgaagg cgtgcgtaac tctctctatg tatcaggctg
    27541 tatgtttcac tgcgagggat gttataatgt tgcgacttgg tcttttaatg ctggcattcc
    27601 ctatacagca gaattagaag agcagatcat ggcagatctt gctcagccct atgttcaagg
    27661 tttgactttg ttgggagggg agccttttct taatactggg attctcttgc cacttgttaa
    27721 gcggattcgg aaggaattgc cagacaagga catctggtcc tggaccggct acacttggga
    27781 agaaatgatg ttggaaactc cagataaact ggaattcttg tcactgattg acattcttgt
    27841 cgatggaaga tatgatcgaa ctaagagaaa tcttatgctc cagtttcgag gttcatctaa
    27901 ccaacgaatt atcgatgtgc aaaaatcgct caaaagtggg caagtagtga tttgggacaa
    27961 gctcaatgac ggaaaagaaa gctatgaaca ggtgaagaga gaatgaagaa aaaatgaaga
    28021 aaaaagactt agtagaccaa ctagtctcag agatcgagac ggggaaagtc aggacactgg
    28081 gaatatacgg tcatggagct tcaggtaaat caacctttgc acaggaattg taccaagctt
    28141 tagattctac tacagtaa
//
LOCUS       DAVLRT010000020        24057 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLRT010000020 DAVLRT010000000
VERSION     DAVLRT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24057
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.020"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..440
                     /locus_tag="VXM85_000854"
     CDS             <1..440
                     /locus_tag="VXM85_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1345467.1"
                     /translation="CLEADNQEQLFDQVATLLEEREIVTPTYREALITREKSFPTGLD
                     MEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINN
                     SSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAETTA"
     gene            478..783
                     /locus_tag="VXM85_000855"
     CDS             478..783
                     /locus_tag="VXM85_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1345468.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            831..2306
                     /locus_tag="VXM85_000856"
     CDS             831..2306
                     /locus_tag="VXM85_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1345469.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2332..2403
                     /locus_tag="VXM85_000857"
                     /pseudo
     CDS             2332..2403
                     /locus_tag="VXM85_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2701..9387
                     /locus_tag="VXM85_000858"
     CDS             2701..9387
                     /locus_tag="VXM85_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1345470.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTANVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPSKLVLERYVGPGFEVPTYYSNYQAYESGHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGKERIFT
                     VINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEADQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9635..10489)
                     /locus_tag="VXM85_000859"
                     /pseudo
     CDS             complement(9635..10489)
                     /locus_tag="VXM85_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10701..11060)
                     /locus_tag="VXM85_000860"
     CDS             complement(10701..11060)
                     /locus_tag="VXM85_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1345471.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKLSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            11152..12108
                     /locus_tag="VXM85_000861"
                     /pseudo
     CDS             11152..12108
                     /locus_tag="VXM85_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12092..12649
                     /locus_tag="VXM85_000862"
     CDS             12092..12649
                     /locus_tag="VXM85_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1345472.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12642..12896
                     /locus_tag="VXM85_000863"
     CDS             12642..12896
                     /locus_tag="VXM85_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345473.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12908..14962
                     /locus_tag="VXM85_000864"
     CDS             12908..14962
                     /locus_tag="VXM85_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1345474.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLILPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15141..16001
                     /locus_tag="VXM85_000865"
     CDS             15141..16001
                     /locus_tag="VXM85_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1345475.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSINFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQFLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGVLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDQVFKKD"
     gene            16212..16922
                     /gene="ccdA2"
                     /locus_tag="VXM85_000866"
     CDS             16212..16922
                     /gene="ccdA2"
                     /locus_tag="VXM85_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1345476.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            16942..17508
                     /locus_tag="VXM85_000867"
     CDS             16942..17508
                     /locus_tag="VXM85_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1345477.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17519..18631
                     /gene="msrB"
                     /locus_tag="VXM85_000868"
     CDS             17519..18631
                     /gene="msrB"
                     /locus_tag="VXM85_000868"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1345478.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18899..19636
                     /locus_tag="VXM85_000869"
     CDS             18899..19636
                     /locus_tag="VXM85_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1345479.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19633..21324
                     /locus_tag="VXM85_000870"
     CDS             19633..21324
                     /locus_tag="VXM85_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1345480.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            21440..22003
                     /locus_tag="VXM85_000871"
     CDS             21440..22003
                     /locus_tag="VXM85_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1345481.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALTSRPKLIGGARAHYPQDAIRKF"
     gene            22025..>24057
                     /locus_tag="VXM85_000872"
     CDS             22025..>24057
                     /locus_tag="VXM85_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1345482.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNYVNK
                     EIISQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYK
                     RKKIASVFLVGTMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTL
                     GLDTVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPK
                     EEPKSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPA
                     EESKVEQAGEPVAPRKDEQAPVEPENQPEAPEEEKAVEETPKQEESTPDTKAEETVEP
                     KEETVNQSIEQPKVETPAVEKQTEPTEEPKVEQAGEPVAPREDEQAPTAPVEPEKQPE
                     VPEEEKAVEETPKPEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPV
                     LETAKGVYASEPVKQPEVNSETNKLKTAIDALNVDKTELNNTIADAKTKVKEHYSDRS
                     WQNLQTEVTKAEKVAANTDAKQSEVNEAVEKLTATIEKLVELSEKPILTLTSTDKKIL
                     EREAVAKYTLENQNKTKIKSITAELKKGEEVINTVVLTDDKVTTETISAAFKNLEYYK
                     EYTLSTTMIYDRGNGEETETLENQNIQLDLKKVELKNIKRTDLIKYENGKETN"
BASE COUNT         7409 a         4531 c         5311 g         6806 t
ORIGIN      
        1 tttgcttaga agcggataat caagaacaac tctttgatca ggttgcaact ttattggaag
       61 aacgagaaat tgtcactcca acttatcgtg aagccttgat cacgcgtgaa aagtcatttc
      121 caactggttt agatatggaa tttctaggaa aggacttgcc aaatgtagcg attcctcata
      181 cagatattgt tcataatcta gctgagaaag tggtggttgt tcgattagaa aaaccagtaa
      241 cttttcacaa tatgatagct cctgataagg aagtagaagt atccttgctc ttctttatca
      301 tcaataactc aagttcaagt caaacaaata ttctggctca gttgatggac tttttcacag
      361 gaaatggaca tcttgaagac ctatcaaaaa tttccgaacc agaaaaactt tatgcttaca
      421 ttgctgaaac aaccgcttaa tcttgtctat taaaaaataa aatcggagga aatctaaatg
      481 attaaaattc ttgctgcctg cggtgcaggt gttaactcaa gtcaccaaat taaaagtgct
      541 ctagaagaag aactttcaaa ccgtggttat gatgttcact gtgatgcagt catggtgaaa
      601 gatgtaaacg aagaccttat gaaaggttac gatatcttta caccaatcgc tgcaacagac
      661 cttggttttg aaccaggtat cccagttatc gaagctggac caatcttatt ccgtatccca
      721 gcaatgagcg ctccagtatt tgacaatatt gaagcagcta ttaaagaaca tggattaagc
      781 taattctaat ttgttaacat tcatataaca taaaaaaggg aggaactgtt atggatgcaa
      841 tctttgacct aatcggaaag gttttcaatc ccatcttaga aatgggtgga cctgtcatca
      901 tgttgatcat tttgacagta ttggctttac tttttggagt gaaattctcc aaagcgcttg
      961 aaggtggtat caaacttgcc atcgctctta caggtatcgg tgctatcatc ggtatgctaa
     1021 atggtgcttt ctcagcatca cttgcaaaat tcgttgaaaa cactggtatc caattgagta
     1081 ttacagacgt tggttgggca ccactcgcaa caatcacttg gggttctgct tggacactat
     1141 acttcttgct catcatgttg attgtcaacg tagtgatgct tgctatgaag aaaaccgata
     1201 cacttgatgt cgatatcttt gatatctggc acttgtctat cacaggtctc ttgattaaat
     1261 ggtatgctga taacaatggt gtgagtcaag gggtttcact ctttattgct acagcagcta
     1321 tcgtccttgt cggtgtgttg aaaattatca actctgactt gatgaaacct acatttgatg
     1381 accttcttaa cgccccaagt tcatcaccaa tgacatcaac tcacatgaac tacatgatga
     1441 acccagttat catggttttg gataagattt ttgaaaaatt cttcccaggc cttgataaat
     1501 atgactttga tgctgctaaa ttgaacaaga aaatcggttt ctggggatct aaattcttca
     1561 tcggtttcat ccttggtatc gttatcggta ttatgggaac tccacatcca attgcaggtg
     1621 ttgcagatgc agataaatgg cgtcttgtta tcaaaggatg gttgtctctt ggtttgactg
     1681 ccggtgtatc tttggaactc ttctcactta tcggttcatg gttcatcgca gccgtagaac
     1741 cactatcaca aggtattaca aacgttgcta ctaaacgtct tcaaggacgt aaattcaata
     1801 tcggtcttga ctggccattc atcgctggtc gtgctgaaat ctgggcttgt gccaacgtac
     1861 ttgcaccaat catgttgatt gaagcagtgc ttctttcaaa agttggaaat ggtatcttgc
     1921 cacttgcagg tatcatcgct atgggtgtta ctccagctct cttggttgta actcgtggta
     1981 aattgctccg tatgattatc ttcggaacac tcttgttgcc actcttcctt ctttcaggta
     2041 cacttattgc accatttgca acagaacttg ctaaaggtgt aggtgccttc ccagaaggtg
     2101 tgagccaaac tcaattgatt actcactcta ctcttgaagg accaatcgaa aaacttcttg
     2161 gttggacaat tggtaacact acaactggtg atatcaaagc aatccttggt gcagtagtct
     2221 tccttgtatt ctatatcggt atctttgctt ggtacagaaa acaaatgatc aaacgtaacg
     2281 aagagtacgc agcaaaagca aaataatgcg ctcctataaa atataaactt aatgaaaatc
     2341 aaagagcaaa ctaggaagct agccgcaggt tgctcaaaac acagttttga ggttgtagat
     2401 aagactgacg aagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc
     2461 agctcaaaac atggttttga ggttgtagat gaaactgacg aagtcagctc aaaacatggt
     2521 tttgaggttg tagatgaaac tgacgaagtc agtaatcata tctacggcaa ggcgacactg
     2581 acgaagtttg aattggattt tcgcagagtg taaattgttg aaactaggtt gtcataccat
     2641 gtataccact tgggctttgg tagtagctaa ctgcactaaa tataatataa ggagggtttt
     2701 atggggaaag gccattggaa tcggaaaaga gtttatagca ttcgtaagtt tgctgtggga
     2761 gcttgctcag taatgattgg gacttgtgca gttctattag gaggaaatat agctggagaa
     2821 tctgtagttt atgcggatga aacacttatt actcatactg ctgagaaacc taaagaggaa
     2881 aaaatgatag tagaagaaaa ggctgataaa gctttggaaa ctaaaaatgt agttgaaagg
     2941 acagaacaaa gtgaacctag ttcaactgag gctattgcat ctgagaagaa agaagatgaa
     3001 gccgtaactc caaaagagga aaaagtgtct gctaaaccgg aagaaaaagc tccaaggata
     3061 gaatcacaag cttcaagtca agaaaaaccg ctcaaggaag atgctaaagc tgtaacaaat
     3121 gaagaagtga atcaaatgat tgaagacagg aaagtggatt ttaatcaaaa ttggtacttt
     3181 aaactcaatg caaattctaa ggaagccatt aaacctgatg cagacgtatc tacgtggaaa
     3241 aaattagatt taccgtatga ctggagtatc tttaacgatt tcgatcatga atctcctgca
     3301 caaaatgaag gtggacagct caacggtggg gaagcttggt atcgcaagac tttcaaacta
     3361 gatgaaaaag acctcaagaa aaatgttcgc cttacttttg atggcgtcta catggattct
     3421 caagtttatg tcaatggtca gttagtgggg cattatccaa atggttataa ccagttctca
     3481 tacgatatca ccaaatacct tcataaagat ggtcgtgaga atgtgattgc tgtccatgca
     3541 gtcaacaaac agccaagtag ccgttggtat tcaggaagtg gtatctatcg tgatgtgact
     3601 ttacaagtga cagataaggt gcatgttgag aaaaatggga caactatttt aacaccaaaa
     3661 cttgaagaac aacaacatgg caaggttgaa actcatgtga ccagcaaaat cgtcaatacg
     3721 gacgacaaag accatgaact tgtagccgaa tatcaaatcg ttgaacgagg tggtcatgct
     3781 gtaacaggct tagttcgtac agcgagtcgt accttaaaag cacatgaatc aacaagccta
     3841 gatgcgattt tagaagttga aagaccaaaa ctctggaccg ttttaaatga caaacctgcc
     3901 ttgtacgaat tgattacgcg tgtttaccgt gacggtcaat tggttgatgc taagaaggat
     3961 ttgtttggtt accgttacta tcactggact ccaaatgaag gtttctcttt gaatggtgaa
     4021 cgtattaaat tccatggagt atccttgcac cacgaccatg gggcgcttgg agcagaagaa
     4081 aactataaag cagaatatcg ccgtctcaaa caaatgaagg agatgggagt taactccatc
     4141 cgtacaaccc acaaccctgc tagtgagcaa accttgcaaa tcgcagcaga actaggttta
     4201 ctcgttcagg aagaggcctt tgatacttgg tatggtggca agaaacctta tgactatgga
     4261 cgtttctttg aaaaagatgc cactcaccca gaagctcgaa aaggtgaaaa atggtctgat
     4321 tttgacctac gtaccatggt cgaaagaggc aaaaataacc ctgctatctt catgtggtca
     4381 attggtaatg aaataggtga agctaatggt gatgcccact ctttagcaac tgttaaacgt
     4441 ttggtcaagg ttatcaagga tgttgataag actcgctatg ttaccatggg agcagataaa
     4501 ttccgtttcg gtaatggtag cggagggcat gagaaaattg ctgatgaact cgatgctgtt
     4561 ggatttaact attctgaaga taattacaaa gcccttagag ctaagcatcc aaaatggttg
     4621 atttatggat cagaaacatc ttcagctacc cgtacacgtg gaagttacta tcgccctgaa
     4681 cgtgaattga aacatagcaa tggacctgag cgtaattatg aacagtctga ctatgggaat
     4741 gatcgtgttg gttgggggaa aacagcaacc gcttcatgga cttttgaccg tgacaacgct
     4801 ggctatgctg gacagtttat ctggacaggt acggactata ttggtgaacc tacaccatgg
     4861 cacaaccaaa atcaaactcc tgttaagagc tcttactttg gtatcgtaga tacagccggc
     4921 attccaaaac atgacttcta cctctaccaa agccaatggg tttctgttaa gaagaaaccg
     4981 atggtacacc ttcttcctca ctggaactgg gaaaacaaag aattagcatc caaagtagct
     5041 gactcagaag gtaagattcc agttcgtgct tattcgaatg cttctagtgt agaattgttc
     5101 ttgaatggaa aatctcttgg tcttaagact ttcaataaaa aacaaaccag cgatgggcgg
     5161 acttaccaag aaggtgcaaa tgctaatgaa ctttatcttg aatggaaagt tgcctatcaa
     5221 ccaggtacct tggaagcaat tgctcgtgat gaatctggca aggaaattgc tcgagataag
     5281 attacgactg ctggtaagcc agcggcagtt cgtcttatta aggaagacca tgcgattgca
     5341 gcagatggaa aagacttgac ttacatctac tatgaaattg ttgacagcca ggggaatgtg
     5401 gttccaactg ctaataatct ggttcgcttc caattgcatg gccaaggtca actggtcggt
     5461 gtagataacg gagaacaagc cagccgtgaa cgctataagg cgcaagcaga tggttcttgg
     5521 attcgtaaag catttaatgg taaaggtgtt gccattgtca aatcaactga acaagcaggg
     5581 aaattcacac tgacagccca ttcagatctc ttgaaatcta gtcaagtcac tgtctttact
     5641 ggtaaaaaag aaggacaaga gaagactgtt ttggggacag aagtgccaaa agtacagacc
     5701 attattggag aggcacctga aatgcctacc actgttccgt ttgtatacag tgatggtagc
     5761 cgtgcagaac gtcctgtaac ctggtcttca gtagatgtga gcaagcctgg tattgtaacg
     5821 gtgaaaggta tggctgacgg acgagaagta gaagctcgtg tagaagtgat tgctcttaaa
     5881 tcagagctac cagttgtgaa acgtattgct ccaaatactg acttgaattc tgtagacaaa
     5941 tctgtttcct atgttttgac tgatggaagt gttgaagagt atgaagtgga caagtgggag
     6001 attgccgaag aagataaagc taagttagca attccaggtt ctcgtattca agcgaccggt
     6061 tatttagaag gtcaaccaat tcatgcaacc cttgtggtag aagaaggcaa tcctgcggca
     6121 cctgcagtac caactgtaac ggttggtggt gaagctgtca caggtcttac tagtcaaaaa
     6181 ccaatgcaat accgcactct tgcttatgga gctaagttgc cagaagtcac agcaagtgct
     6241 aaaaatgcag ctgttacagt tcttcaagca agcgcagcaa acggcatgcg tgcgagcatc
     6301 tttattcagc ctaaagatgg tggccctctt caaacctatg caattcaatt ccttgaagaa
     6361 gcgccaaaaa ttgctcactt gagcttgcaa gtggaaaaag ctgacagtct caaagaagac
     6421 caaactgtca aattgtcggt tcgagctcac tatcaagatg gaacgcaagc tgtattacca
     6481 gctgataaag taaccttctc tacaagtggt gaaggggaag tcgcaattcg taaaggaatg
     6541 cttgagttgc ataagccagg agcagtcact ctgaacgctg aatatgaggg agctaaaggc
     6601 caagttgaac tcactatcca agccaatact gagaagaaga ttgcgcaatc catccgtcct
     6661 gtaaatgtag tgacagattt gcatcaggaa ccaagtcttc cagcaacagt aacagttgag
     6721 tatgacaaag gtttccctaa aactcataaa gtcacttggc aagctattcc gaaagaaaaa
     6781 ctagactcct atcaaacatt tgaagtacta ggtaaagttg aaggaattga ccttgaagcg
     6841 cgtgcaaaag tctctgtaga aggtatcgtt tcagttgaag aagtcagtgt gacaactcca
     6901 atcgcagaag caccacaatt accagaaagt gttcggacat atgattcaaa tggtcacgtt
     6961 tcatcagcta aggttgcatg ggatgcgatt cgtccagagc aatacgctaa ggaaggtgtc
     7021 tttacagtta atggtcgctt agaaggtact caattaacaa ctaaacttca tgttcgcgta
     7081 tctgctcaaa ctgagcaagg tgcaaacatt tctgaccaat ggaccggttc agaattgcca
     7141 cttgcctttg cttcagattc aaatccaagc gacccagttt caaatgttaa tgacaagctc
     7201 atttcctaca ataaccaacc agccaatcgt tggacaaact ggaatcgtag taatccagaa
     7261 gcttcagtcg gtgttctgtt tggagattca ggtatcttga gcaaacgctc cgttgataat
     7321 ctaagtgtcg gattccacga agaccatgga gttggtgcac cgaagtctta tgtgattgag
     7381 tattatgttg gtaagactgt cccaacagct cctaaaaacc ctagttttgt tggtaatgag
     7441 gaccatgtct ttaatgattc tgccaactgg aaaccagtta ctaatctaaa agcccctgct
     7501 caactcaagg ctggagaaat gaaccacttt agctttgata aagttgaaac ctatgctatt
     7561 cgtattcgca tggttaaagc agataacaag cgtggaacgt ctatcacaga ggtacaaatc
     7621 tttgcgaaac aagttgcggc agccaaacaa ggacaaacaa gaatccaagt tgacggcaaa
     7681 gacttagcaa acttcaaccc tgatttgaca gactactacc ttgagtctgt agatggaaaa
     7741 gttccggcag tcacagcaaa tgttagcaac aatggtctcg ctaccgtcgt tccaagcgtt
     7801 cgtgaaggtg agccagttcg tgtcatcgcg aaagctgaaa atggcgacat cttaggagaa
     7861 taccgtctgc acttcactaa ggataagaac ttactttctc ataaaccagt tgctgcggtt
     7921 aaacaagctc gcttgctaca agtaggtcaa gcacttgaat tgccgactaa ggttccagtt
     7981 tacttcacag gtaaagacgg ctacgaaaca aaagacctga cagttgaatg ggaagaagtt
     8041 ccagcggaaa atctgacaaa agcaggtcaa tttactgttc gaggccgtgt ccttggtagt
     8101 aaccttgttg ctgaggtcac tgtacgagtg acagacaaac ttggtgagac tctttcagat
     8161 aaccctaact atgatgaaaa cagtaaccag gcctttgctt cagcaaccaa tgatattgac
     8221 aaaaactctc atgaccgcgt tgactatctc aatgacggag atcattcaga aaatcgtcgt
     8281 tggacaaact ggtcaccaac accatcttct aatccagaag tatcagcggg tgtgatcttc
     8341 cgtgaaaatg gtaagattgt agaacggact gttgctcaag ccaaacttca cttctttgca
     8401 gatagtggta cggatgcacc atctaaactc gttttagaac gctatgtcgg cccaggcttt
     8461 gaagtaccta cctactattc aaactaccaa gcctacgaat ctggacatcc atttaacaat
     8521 ccagaaaatt gggaagctgt gccttatcgt gcggataaag acatcgcagc tggtgatgaa
     8581 atcaacgtaa catttaaagc tgtcaaagcc aaagccatga gatggcgtat ggagcgtaaa
     8641 gctgacaaga gcggtgttgc gatgattgag atgaccttcc ttgcaccaag tgaattgcct
     8701 caagaaagca cgcaatcaaa gattcttgta gatggaaaag aacttgctga tttcgctgaa
     8761 aatcgtcaag actatcaaat tacctataaa ggtcaacggc caaaagtctc agttgaagaa
     8821 aacaatcaag tagcttcaac tgtggtagat agtggagaag atagccttcc agtacttgtt
     8881 cgcctcgttt cagaaagtgg aaaacaagtc aaggaatacc gtatccagtt gactaaggaa
     8941 aaaccagttt ctgctgtaca agaagatctt ccaaaactcg aatttgttga aaaagatttg
     9001 gcctacaaga cagttgagaa aaaagattca acactgtatc taggtgaaac tcgtgtagaa
     9061 caagaaggaa aagttggaaa agaacgtatc tttacagtga ttaatcctga tggaagtaag
     9121 gaagaaaaac tccgtgaagt ggtagaagtt ccgacagacc gcatcgtctt ggttggaacc
     9181 aaaccagtag ctcaagaagc taaaaaacca caagtgtcag aaaaagcaga tacaaaacca
     9241 attgattcaa gtgaagctga tcaaactaat aaagcccagt taccaaatac aggtagtgcg
     9301 gcaagccaag cagcagtagc agcaggttta gctcttctag gtttgagtgc aggattagta
     9361 gttactaaag gtaaaaaaga agactagaaa ttttgatacc ttctttatca ttaaagacca
     9421 aagaaaaact cttgtcctaa atagaagata aagagtctgg aacaaaatag ttctcaacca
     9481 caaaaaagct agatatttcc aattgcggaa ctctagcttt ttaattttga gtcgttctct
     9541 tattgtatag tagattgaaa ctagaatagt acacttctac ttctaaaaca ttgttagaaa
     9601 tcaatttgac tgtccttatc gatttgtcct ggtcttattt catttcacta tgttttagga
     9661 ggttattggc cataagtacc aatcccatgt caattctcac ttgacgcttc cctctcagat
     9721 tacatctctt gtaacccaaa caagccttta tctgcccaaa gacaggtttc acatcaatct
     9781 tgcgttgagc gaaatctgtc taccttcagg agataaaagc gcctgacatt ctttagcttt
     9841 caagttttga tagcgttcgt tcatatacag tcccttttga gggactgatt caggttcatc
     9901 agcgtagtaa accttgattt cctgttgaaa gtctgtctgt gttttctgat gtttggtatg
     9961 gtgaaaacga taataccagc catcaggatg tgtgtagcta tcctccttgt cattatagtg
    10021 ccaattcgct aagtttttag ctgactgttt atgtcctctc ttttgtttct tatcaaacat
    10081 ggcatattta atcagaaggg gaggatttct tcttgaaaat agacctgtat ctgttcttga
    10141 agtttggcgg agaatttctc tgtcgttttc ttccacacaa aactatactt gttggcattg
    10201 gcttcaatct tagttccgtc aataaacaaa caatctaagg tcactaactc ttccattttt
    10261 caatttttcg accagagaaa atcccttgtg aataggcaaa tagaagagta gatacaagca
    10321 ttttaaggtg ataagacggg cgatcaaagg catgatagaa ggcgtagaag tgatgttcct
    10381 ccaaggtatt caccactttt tcaatagtaa agacgagatg gtcttgtggt aagaaggaac
    10441 tgatttctag tggtaaagtt gtttgatttg tgttatagtg aatatgcatg ggatagcctt
    10501 tctaaatggt ttattgggca attctatttt accaagacta tcgtaaccat gcaaaaaaag
    10561 caacggcaaa tccgttgctt tttttggaga taagagacta ttgtcccaga ctcttttctt
    10621 tgtataaaat tgtatagtgt tattgtctat actattttgt agagaaagca aaaatcccta
    10681 gaaaatgctt agggattcga ctatttcatt aagaacaatt caacaatcat ttctatatac
    10741 atgataaggg ttaataagaa tcctgcacgc cagaagaatt tgatgaattt aggatagtaa
    10801 aagctgagtt ttttcaaaag gaagaaaaag acgagaataa tggctaggat tgagagggct
    10861 agccccagtc tagggaggaa attatgggta aaggttttag ctgtaatcag gtaatattca
    10921 aataccaaga ccggaaaagc caaatccgca aagtttcgtc ctagtttttt taatctaaaa
    10981 agtttggtta ggataatgca gacgactaag gtcagtatca ataataaaat agatgctaat
    11041 ttcattaaaa tcatacccat attgtatcat aaaaaaaagc taatggaaac gagaaacaga
    11101 ggaatttata ggaaagtcag gcttttgaga ttgtgggtga tttttgttat aatgaaagtt
    11161 ataaaatctt ataatacctt gaatgattat tatcgaaaac tctttggaga aaagactttt
    11221 aaagtcccta ttgatgcgga atttgactgt cccaatcgtg atggaactgt ggctcatgga
    11281 ggctgtactt tttgtacggt ttcaggttct ggagatacta ttgtagcccc agatgcgcct
    11341 atccgtgagc aattttataa ggaaattgac tttatgcacc gtaagtggcc ggatgttcag
    11401 aagtatctgg tttattttca aaattttacc aacacccatg aaaaggtgga agttattcga
    11461 gagcgttatg aacaggctat caatgagcca ggtgtggtag gaatcaatat tggaatgcgc
    11521 ccagactgtt taccagacga aaccatcgaa tatttggctg agttatcgga gtgcatgcat
    11581 gtgacggttg aattgggctt gtagactact tatgaagcaa cttctgacct gattaaccgt
    11641 gcgtattctt atgaattgta ggtggaaacg gtcaagcgtt tgagaaagta tcccaagatt
    11701 gagattgttt cccatttgat taatggtttg cctggtgaaa cccatgagat gatggttgaa
    11761 aatgtccgcc gttgtgtcac gaataacgat attcaaggga ttaaactgca tttgctccat
    11821 cttatgacaa atactcgtat gcaacgagat taccatgagg gacgtttaca attgatgagt
    11881 caggacgaat atgtcagggt catctgtgac caactggaaa tcattcccaa acatatcgtc
    11941 atccatcgaa tcacaggaga tgcacctagg gatatgctga ttgggcttat gtggagcctc
    12001 aataaatggg aagctctcaa cagcattgag atggagatgc gacgtcgtgg aagtgttcaa
    12061 ggatgcaagg ctgtaaaaca ggagtttgaa aatgaaaaga ccacttgaga tggcacatga
    12121 ttttttggct gaggtcgtga caaaagagga tgtcgtagtg gatgcgacta tgggaaatgg
    12181 tcatgacacg ctttttttag ccaagctagc caagcaagtc tatgcctttg atattcagaa
    12241 gcaagccttg gaaaagaccc aagagcgttt gcatcaggct ggcttgacaa atgcccagtt
    12301 aatcttgcaa ggccatgaga cactggacca gtttgtgaca aaagctaagg cagggatttt
    12361 taatttgggc tatttgccgt cagctgataa gtctgtcatc acccgaccgc agacaacgat
    12421 tgaggcatta gaaaagctat gtggcttact tgtcaagggt ggacgaattg ctattatgat
    12481 ttactatggt catgaaggag gcgacctcga gagagatgct gtcttggatt ttgtaatcca
    12541 gttgaaccaa caagagtaca cagctgccat ttaccgaact ttaaaccaag tcaacaaccc
    12601 gccgttttta gtgatgattg aaaaattaga gagatacaga catggataaa caatacctac
    12661 acgaaaagct ggatgccatg cgccagaatt ttgttgaatc aacccaccac gaacgatcgg
    12721 tgggcgtgct agaccaagcg catatgagca aaaaaatgct taaaatcaag aaaaaattag
    12781 ttgctcttga aatggaacgg tgccagagaa aaattgagca caaagactgt tccaagattg
    12841 accaaaaaat caaagagcag aaggagatat ttgaatcctg ttgtaaaaaa gattaaggag
    12901 gacgtgcgtg gaattactga tttacctcat cctattttta ctggttttga ttgtctcaag
    12961 tacaaccaat aagctcctgc cctttttgcc tctccctttg gtgcaaattc ttttgggaat
    13021 tgtgattggt ctctttttac ccaataccga ctttcacctc aatacggagt tgtttttggc
    13081 cctggttatc ggacccttgc ttttccgaga ggctgaagaa gcagatgtta cggctatttt
    13141 aaaacactgg cgaatcattg tttatctcat atttccagtg atttttatct cgaccctgag
    13201 tttgggtggc ttggcccatc ttctttggtt cagccttccc ttggcagctt gcttggctgt
    13261 tggggcagcc cttggtccta cggacttggt ggcctttgcc tctctttcgg agcgttttag
    13321 ctttcctaag cgcgtgtcca atattcttaa gggcgaagga ctcttgaatg atgcttctgg
    13381 tttggtggct tttcaggtag ctttgacagc ttggacaact ggagcttttt ctctggggca
    13441 agctagcagt tcgctcatct tttcaatcct aggcggtttt ttaattggat ttttaacagc
    13501 catgaccaac cgcttcctcc ataccttctt gctaagtgtg cgcgcaacgg atattgccag
    13561 tgaactttta ttagaattaa gtttgcctct agtgaccttc tttctggcag aagaagttca
    13621 tgtttcaggg attattgccg tcgtagttgc tggaatttta aaggcaagtc gtttcaagaa
    13681 aatcacgctc ctcgaagccc aagtggatac ggtgaccgag acggtctggc atacagtgac
    13741 ctttatgctc aacggttctg tctttgtgat tttagggatg gagttggaaa tgatagcaga
    13801 acctatcttg accaatccaa tctataatcc tctactttta ttgctatctc tcatcgccct
    13861 tacctttgtc ctctttgtca ttcgttttat tatgatctat ggctattatg cctatagaac
    13921 ccgacgcctc aagaaaaagc taaataagta tatgaaggac atgtttctct tgaccttttc
    13981 aggtgttaag ggaacggtgt cgattgctac gattctcttg ataccaagta atctagaaca
    14041 agagtatcct cttttgcttt tccttgttgc aggtgtgacg cttgtcagct ttttaacagg
    14101 tctcttgatc ttgcctcatc tttctgatga agaggaagaa agcaaggatt atctcatgca
    14161 tatcgccatt ttgaatgaag taacgctaga gttggaaaaa gagttggaag acaccagaaa
    14221 taaacttccc ctctatgcgg ctattgacaa ttatcatgga cgtattgaaa atctcatttt
    14281 aagccaagaa aaccaggatg atcaagaaga ctgggctgct ttgaaactct tgattcttag
    14341 tattgaaagt gatggtttgg aacaggccta tgaagagggg aacattagca atcgtgctta
    14401 ccgagtttac caacgttatc tgaaaaatat agaacaagga atcaatcgta aacttgcctc
    14461 aagactgacc tattattttc ttgtttcctt gaggatttta cgttttctcc ttcatgaagt
    14521 ttttactctt ggaaagacct tccgtagctg gaaggacaag gagcaaagcc gtctccgtgc
    14581 tcttgattat gaccaaattg cagagctcta tcttgccaat acggagatga ttattgaaag
    14641 tttggaaaac ctgaagggag tctacagacg ctctttgatt agttttatgc aggagtctcg
    14701 tcttcgagaa acaactatta tcagcagtgg tgcatttgtc gaacgggtta tcaatcgtgt
    14761 caaacccaac aatatcaatg aaatgctgag aggctattat ctggagcgca agttgatttt
    14821 cgaatacgaa gaaaaacgat taattacaac taagtatgcc aagaaattac gacaaaatgt
    14881 aaataactta gagaactatt ccttgaagga agctgccaat accctgccgt atgatatggt
    14941 ggaattggta agaagaaatt agttaatact cttcgaaaat ctcttcaaac cacgtcagcg
    15001 tcgccttacc gtatgtatgt gactgacttc gtcagtctta tctacaacct caaaacagtg
    15061 ttttgagcag cttgcggcta gctttctagt ttgctctttg attttcattg agtataagat
    15121 tataaatgaa ggagtatgac atgaaaaagt ggtggaaaga gctgatagac aagcctttat
    15181 taaaagcttt tttgcattat tatcaagcat cagatagtga gttgaccagt gtcgcagtag
    15241 cctactattg gttgatttcg attttccccc tgcttttggt ggtggtcaat atcctcccct
    15301 attttcagat tcctgtggga gaatttctgg gctttatgaa agacgtcctg cccccaagcc
    15361 tctatgaagg tgtggaaaaa atagccagag aagtcttgac gcaaccttca acaggtttat
    15421 tgagtttttc tgttttatcg gcgttatgga gtttttcaaa atctataaat ttcttgcaaa
    15481 aagcttttaa caaggcttat ggcgtcgaaa agagtcgtgg acttatttcc catcagatgc
    15541 taagtctcct agtcagtttt ggcttgcagt tcctctttgc gtttgccttg tttttgatct
    15601 tatttggtca gatgattttg gctttacttg ctcattattg gacaaaagat ggcattatct
    15661 atcaggcttt acaagggttg gcaggtcctc tgatttacgc cctcctcttt gctatcttgg
    15721 tcatgcttta ttattttctt cccaatctat ctaacaggaa aattagctat accctaccag
    15781 gcagtgcctt tgttctcttg gtgattttag gtgtactaac tctgttctct agttatctca
    15841 attactatgt ccatcatttg gtggatgtcc gaattttagg ttccgttctt cttgttgtta
    15901 tgatgttttg gtttatcttg attgctaaaa ttgttatcct aggcgcggtg atcaatgcca
    15961 gtatgcagag tttgaaagat caggtcttta aaaaagacta actatttatc cattacaaaa
    16021 gcgcatagta tcaaggcttt atagaatctt gatactatgc gcttttgtgc tttgacagtt
    16081 ttttgtatag tttttgtcca agatttctaa atcatctgat taaattccta ttttccccta
    16141 tcattgtccc tgttttttcg aaaatggaag gagtataata aatctatcaa taaaatgata
    16201 ggaggaactt tatgggtcat attttctttt ttctaagtgt ctttttggca gggattctat
    16261 ccttcttttc tccttgtatc ttacctttgt taccggtcta tacaggagtg ttactagatg
    16321 ataaggatgg tgctcaggct tctagcggca aattttcaat ctcagttact agtttattac
    16381 gaactctggc ctttatagca ggaatttcct ttatatttat tttgttgggc tatggagctg
    16441 gttttttagg cgatttgctt tatgcttctt ggttccaata tcttactggg gcaattatta
    16501 tccttcttgg tttgcaccaa atggagattc tacactttaa ggggctttat aaggaaaaga
    16561 ggctacaact gcaaggacag gggcaaaatg gtaagggcta tagtcaggca tttttattgg
    16621 gcttgacctt tagttttgct tggacgcctt gcgtggggcc ggttctgggg tctgttttgg
    16681 ccttggcggc ttcaggtggt tcaggagctt ggcagggagc tggtctcatg ttggtgtata
    16741 cgctgggctt ggcgctacca ttcttgcttc tagctctgac ctctagttat gttttgaaac
    16801 atttccgaaa acttcatccc tatctcggaa tcctcaaaaa agtgggtggt tttctcatta
    16861 ttgtgatggg attcttggtt ctgtttggaa atgcttcaat tttaagtcaa ttatttgaat
    16921 aaaatggaaa ggaatatcaa tatgaaaaaa tggcaaacat gtgttcttgg agcaggttcg
    16981 ctcctttgtt tgacggcttg ttcaggcaag tccgtgacta gtgaacacca aacgaaagat
    17041 gaaatgaaga cggagcagac agctagtaaa acaagcgcag ctaaagggaa agaggtggct
    17101 gattttgaat tgatgggagt agatggcaag acctaccgtt tatctgatta caagggcaag
    17161 aaagtctatc tcaaattctg ggcttcttgg tgttccatct gtctggctag tcttccagat
    17221 acggatgaga ttgctaaaga agctggtgat gactatgtgg tcttgacagt agtgtcacca
    17281 ggacataagg gagagcaatc tgaagcggac tttaagaatt ggtataaggg attggattat
    17341 aaaaatctcc cagtcctagt tgacccatca ggcaaacttt tggaaactta tggtgtccgt
    17401 tcttacccaa cccaagcctt tatagacaaa gaaggcaagc tggtcaaaac acatccagga
    17461 ttcatggaaa aagatgcaat tttgcaaact ttgaaggaat tagcctagga ggcgtcttat
    17521 gaatgataag ttaaaaatct tcttgttgct aggagtattt tttctagcca taaccggttt
    17581 ctatgttcta ttgatacgaa atgcagggca gacagatgcc tcgcaaattg aaaaggcggc
    17641 agttagccaa ggaggaaaag cagtgaaaaa aacagaaatt agtaaagacg cagacttgca
    17701 cgaaatttat ctagctggag gttgtttctg gggagtggag gaatatttct cacgtgttcc
    17761 cggggtgacg gatgccgttt caggctatgc aaatggtaga ggagaaacaa ccaagtacga
    17821 attgattaac caaacaggtc atgcagaaac cgtccatgtc acctatgatg ccaagcaaat
    17881 ttctctcaag gaaatcctgc ttcactattt ccgcattatc aatccaacca gcaaaaataa
    17941 acaaggaaat gatgtgggga cccagtaccg tactggtgtt tattacacag atgacaagga
    18001 tttggaagtg attaaccaag tctttgatga ggtggctaag aaatacgatc aacctctagc
    18061 agttgaaaag gaaaacttga agaattttgt ggtggctgag gattaccatc aagactatct
    18121 caagaaaaat ccaaatggct actgccatat caatgttaat caggcggcct atcctgtcat
    18181 tgatgccagc aaatatccaa aaccaagtga tgaggaattg aaaaagaccc tgtcacctga
    18241 ggagtatgca gttacccagg aaaatcaaac agaacgagct ttctcaaacc gttactggga
    18301 taaatttgaa tccggtatct atgtggatat agcaactggg gaacctctct tttcatcaaa
    18361 agacaaattt gagtctggtt gtggctggcc tagttttacc caacccatca gtccagatgt
    18421 tgtcacctac aaggaagata agtcctacaa tatgacgcgt atggaagtgc ggagccgagt
    18481 aggagattct caccttgggc atgtctttac ggatggtcca caggacaagg gcggcttacg
    18541 ttactgtatc aatagcctct ctatccgctt tattcccaaa gaccaaatgg aagaaaaagg
    18601 ctacgcttat ttactagatt atgttgatta atactcttcg aaaatctctt caaaccacgt
    18661 cagcgtcgcc ttaccgtatg tagggttact gacttcgtca gttttatcta caacctcaaa
    18721 gctatacttt gagctgactt cgtcagtttt atttacaacc tcaaagctat actttgagaa
    18781 acctgtggct atcttcctag tttacttttt gatttttatt gagtataaga ggtctttcct
    18841 aagcagttag aggagaattt tgctatactg atactagtaa gtggtaaagg agcagagcat
    18901 gacctacaca atcttaatcg tagaagatga atatctggta agacaaggtt tgactaaact
    18961 ggtcaatgta gcagcctacg atatggaaat catcggtcag gctgaaaatg gaaggcaggc
    19021 ttgggaattg atccaaaagc aggtgccaga tatcatttta accgatatca acatgcctca
    19081 tctaaatggc atccagttgg ccagtctggt acgagaaacc tatcctcagg ttcatttggt
    19141 ctttttaaca ggttacgatg attttgatta tgccttgtct gctgtcaaac taggtgtgga
    19201 cgactacctg ctcaaaccct tttctcgtca ggatattgag gaaatgttgg ggaaaatcaa
    19261 acaaaaacta gacaaggaag agaaagaaga gcagttacaa gatttattga ctaataggtt
    19321 tgaaggaaac atggcccaga aaatccagtc tcatctggct gatagccaat ttagtttaaa
    19381 gtctttagcc agtgacttag gttttagtcc gacctatctg agttccttga ttaagaaaga
    19441 gttgggcttg ccttttcagg attatctggt gagagaacgt gttaaacaag ccaagctctt
    19501 gcttttaact acagatctga agatttatga gatcgcagag aaggttggtt ttgaagatat
    19561 gaactatttt acccaacgtt ttaagcagat tgcaggtgtg acacctcgtc agtttaagaa
    19621 gggagaagac cgatgaagcg ttcttctctt ttagttagaa tggttatttc catctttctg
    19681 gtctttctca ttctcctagc tctggttgga actttctact atcaatcaag ttcttcagcc
    19741 attgaggcca ccattgaggg caacagccaa acgaccatca gccagactag ccactttatt
    19801 cagtcttata tcaaaaaact agaaaccacc tcgactggtt tgacccagca gacggatgtt
    19861 ctggcctatg ctgagaatcc cagtcaagac aaggtcgagg gaatccgaga tttgtttttg
    19921 accatcttga agtcagataa ggacttgaaa actgttgtgc tggtgaccaa atctggtcag
    19981 gtcatttcta cagatgacag tgtgcagatg aaaacttcct ctgatatgat ggctgaggat
    20041 tggtaccaaa aggctattca tcagggagct atgcctgttt tgactccagc tcgtaaatca
    20101 gatagtcagt gggtcatttc tgtcactcaa gaacttgttg atgcaaaggg agccaatctt
    20161 ggtgtgcttc gtttggatat ttcttatgaa actctggaag cctatctcaa tcaactccag
    20221 ttggggcagc agggctttgc cttcattatc aatgaaaacc atgaatttgt ctaccatcct
    20281 caacacacag tttatagttc gtctagcaaa atggaggcta tgaaacccta catcgagaca
    20341 ggtcagggtt atactcctgg tcacaaatcc tacgtcagtc aagagaagat tgcaggaact
    20401 gattggacgg tacttggcgt gtcatcattg gaaaagttag accaggttcg gagtcagctc
    20461 ttgtggacct tgcttggggc cagtgtcaca tctcttcttg tctgtctctg cttagtgtgg
    20521 ttcagtctta aacgctggat tgctcctttg aaggatttga gagaaaccat gttggaaatt
    20581 gcttctggtg ctcaaaatct tcgtgccaag gaagttggtg cctatgaact gagagaagta
    20641 actcgccaat ttaatgccat gttggatcag attgatcagt tgatggtagc tattcgtagc
    20701 caggaagaaa cgacccgtca gtaccaactt caagcccttt cgagccagat taatccacat
    20761 ttcctctata acactttgga caccatcatc tggatggctg aatttcatga tagtcagcga
    20821 gtggtgcagg tgaccaagtc cttggcaacc tatttccgct tggcgctcaa tcaaggcaag
    20881 gacttgattt gtctctctga cgaaatcaat catgtccgcc agtatctctt tatccagaaa
    20941 caacgctatg gagataagct ggaatacgaa attaatgaaa atgttgcctt tgataattta
    21001 gtcttaccca agctggtcct acaacccctt gtagaaaatg ctctttacca tggcattaag
    21061 gaaaaggaag gtcagggcca tattaaactt tctgtccaga aacaggattc gggattggtc
    21121 atccgtattg aggatgatgg cgttggcttc caagatgctg gtgatagtag tcaaagtcaa
    21181 ctcaaacgtg ggggagttgg tcttcaaaat gtcgatcaac ggctcaaact tcattttgga
    21241 gccaattacc agatgaagat tgattctaga ccccaaaaag gaacgaaagt tgaaatatat
    21301 ataaatagaa tagaaactag ctaactccca gtcaattctg ggagttttat gcgtaattgg
    21361 gcaaactttc taggttgtct atcttgctaa aaacaagaaa aaacggtatg atagaagagt
    21421 gacaacagag gtaacagaga tgaacgagac acagattcaa agagaaacac gtcaggtagt
    21481 ggaagatgtt ttagaaaaga ccaatttgaa acagggggcc ctttttgttt taggtctatc
    21541 ttctagtgag gtgctaggtg gtcagattgg gaaagaatcc agtcaagaga ttggtgagct
    21601 gatagtggag actatcttgg gtattctagg tagtagaggt atccatttag cagttcaagg
    21661 ttgtgagcat gtcaatcggg ctctcgttgt tgaacgtcag gtggcagagc agtttggtct
    21721 ggaaattgtc agtgtccatc ctactcttca tgcaggaggt tcggggcagt tggcagcctt
    21781 taagtttatg caggatccag ttgaggttga atttatcaag gctcatgctg gattggatat
    21841 cggagacact gcaattggca tgcatgtcaa gcatgttcag gttccgattc gccctatttt
    21901 gagagagatt ggtcatgccc atgtaacggc actgactagt cgtccaaaat taatcggagg
    21961 tgcgcgtgcg cactatccgc aagatgctat taggaagttt tgagaatgaa taaaggagat
    22021 aaaagtgttt aaaaaagacc gtttttcaat tcgtaagatt aagggagttg taggctctgt
    22081 atttcttgga agccttttga tggctccttc tgtagtggac gcagccacct atcactatgt
    22141 aaattatgta aataaagaga ttatttcaca agaagctaaa gatttaattc agacaggaaa
    22201 gcctgacagg aatgaagttg tatatggttt ggtgtatcaa aaagatcagt tgcctcaaac
    22261 agggacagaa gcatctgttt tgacagcttt tggtttgctg actgttggga gcttgctttt
    22321 aatctacaag agaaagaaaa ttgctagcgt ctttctagtt ggaactatgg gattggtagt
    22381 tcttcctagt gcaggggctg tagacccagt tgcgacccta gcactggcta gtcgagaggg
    22441 tgttgttgaa atggagggct atcgctatgt tggttatcta tcaggtgaca tcctcaaaac
    22501 gcttggcttg gacactgttt tagaagaaac ctcagcaaaa cctggagagg tgactgtagt
    22561 cgaagttgag actcctcaat caacaacaaa tcaggagcaa gctaggacag aaaaccaagt
    22621 agtagagaca gaggaagctc caaaagaaga agcacctaaa acagaagaaa gtccaaagga
    22681 agaaccaaaa tcggaggtaa aacctactga cgacaccctt cctaaagtag aagaggggaa
    22741 agaagattca gcagaaccag ctccagttga agaagtaggt ggagaagttg agtcaaaacc
    22801 agaggaaaaa gtagcagtta agccagaaag tcaaccatca gacaaaccag ctgaggaatc
    22861 aaaagttgaa caagcaggtg aaccagtcgc gccaagaaaa gacgaacagg caccagtcga
    22921 gccagaaaat caaccagaag ctcctgaaga agagaaggct gtagaggaaa caccgaaaca
    22981 agaagagtca actccagata ccaaggctga agaaactgta gaaccaaaag aggagactgt
    23041 taatcaatct attgaacaac caaaagttga aacgcctgct gtagaaaaac aaacagaacc
    23101 aacagaggaa ccaaaagttg aacaagcagg tgaaccagtc gcgccaagag aagacgaaca
    23161 ggcaccaacg gcaccagttg agccagaaaa gcaaccagaa gttcctgaag aagagaaggc
    23221 tgtagaggaa acaccgaaac cagaagataa aataaagggt attggtacta aagaaccagt
    23281 tgataaaagt gagttaaata atcaaattga taaagctagt tcagtttctc ctactgatta
    23341 ttctacagca agttacaatg ctcttggacc tgttttagaa actgcaaaag gtgtctatgc
    23401 ttcagagcct gtaaaacagc ctgaggtaaa tagcgagaca aataaactta aaacggctat
    23461 tgacgctcta aacgttgata aaactgaatt aaacaatacg attgcagatg caaaaacaaa
    23521 ggtaaaagaa cattacagtg atagaagttg gcaaaacctc caaactgaag ttacaaaggc
    23581 tgaaaaagtt gcagctaata cagatgctaa acaaagtgaa gttaacgaag ctgttgaaaa
    23641 attaactgca actattgaaa aattggttga attatctgaa aagccaatat taacattgac
    23701 tagtaccgat aagaaaatat tggaacgtga agctgttgct aagtatactc tagaaaatca
    23761 aaacaaaaca aaaatcaaat caatcacagc tgaattgaaa aaaggagaag aagttattaa
    23821 tactgtagtt cttacagatg acaaggtaac aacagaaact ataagcgctg catttaagaa
    23881 cctagagtac tacaaagaat acaccctatc tacaactatg atttacgaca gaggtaacgg
    23941 tgaagaaact gaaactctag aaaatcaaaa tattcaatta gatcttaaaa aagttgagct
    24001 taaaaatatt aaacgtacag atttaatcaa atacgaaaat ggaaaagaaa ctaatga
//
LOCUS       DAVLRT010000021        24036 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLRT010000021 DAVLRT010000000
VERSION     DAVLRT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.021"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            99..962
                     /locus_tag="VXM85_000873"
     CDS             99..962
                     /locus_tag="VXM85_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1345483.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            complement(1148..1618)
                     /locus_tag="VXM85_000874"
     CDS             complement(1148..1618)
                     /locus_tag="VXM85_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV1345484.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(1584..2390)
                     /locus_tag="VXM85_000875"
     CDS             complement(1584..2390)
                     /locus_tag="VXM85_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1345485.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(2597..3238)
                     /locus_tag="VXM85_000876"
     CDS             complement(2597..3238)
                     /locus_tag="VXM85_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345486.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(3235..5343)
                     /locus_tag="VXM85_000877"
     CDS             complement(3235..5343)
                     /locus_tag="VXM85_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1345487.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(5395..5691)
                     /locus_tag="VXM85_000878"
     CDS             complement(5395..5691)
                     /locus_tag="VXM85_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1345488.1"
                     /translation="MIGEINHEKISVASLMVCGILATTNALAVWVEGGQWNYGVGWTG
                     TFGYSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            6096..6416
                     /locus_tag="VXM85_000879"
     CDS             6096..6416
                     /locus_tag="VXM85_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1345489.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(6468..7055)
                     /locus_tag="VXM85_000880"
     CDS             complement(6468..7055)
                     /locus_tag="VXM85_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1345490.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQKLVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            7297..7968
                     /gene="sdaAB"
                     /locus_tag="VXM85_000881"
     CDS             7297..7968
                     /gene="sdaAB"
                     /locus_tag="VXM85_000881"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1345491.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            7977..8849
                     /gene="sdaAA"
                     /locus_tag="VXM85_000882"
     CDS             7977..8849
                     /gene="sdaAA"
                     /locus_tag="VXM85_000882"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1345492.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            8871..9503
                     /locus_tag="VXM85_000883"
     CDS             8871..9503
                     /locus_tag="VXM85_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1345493.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            9553..9810
                     /locus_tag="VXM85_000884"
     CDS             9553..9810
                     /locus_tag="VXM85_000884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345494.1"
                     /translation="MKIKEQTRKLAAGCSKHCFKIVDGTDEVSSKHCFKVVDGTDEVS
                     SKHCFEVADRTDEVSSKHCFKVVDGTDEVSNHIRQGDVDVI"
     gene            complement(9896..11746)
                     /locus_tag="VXM85_000885"
     CDS             complement(9896..11746)
                     /locus_tag="VXM85_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1345495.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(11788..12945)
                     /locus_tag="VXM85_000886"
     CDS             complement(11788..12945)
                     /locus_tag="VXM85_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1345496.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKEDIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVINNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNML"
     gene            13003..14169
                     /locus_tag="VXM85_000887"
     CDS             13003..14169
                     /locus_tag="VXM85_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1345497.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            14308..14634
                     /locus_tag="VXM85_000888"
     CDS             14308..14634
                     /locus_tag="VXM85_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1345498.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEHLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            14621..15214
                     /locus_tag="VXM85_000889"
     CDS             14621..15214
                     /locus_tag="VXM85_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1345499.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYATRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            15207..16118
                     /locus_tag="VXM85_000890"
     CDS             15207..16118
                     /locus_tag="VXM85_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1345500.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            16181..17245
                     /locus_tag="VXM85_000891"
     CDS             16181..17245
                     /locus_tag="VXM85_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345501.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIANLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLKSPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(17317..18177)
                     /locus_tag="VXM85_000892"
     CDS             complement(17317..18177)
                     /locus_tag="VXM85_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1345502.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(18299..19285)
                     /locus_tag="VXM85_000893"
     CDS             complement(18299..19285)
                     /locus_tag="VXM85_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1345503.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(19557..21032)
                     /locus_tag="VXM85_000894"
     CDS             complement(19557..21032)
                     /locus_tag="VXM85_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1345504.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(21116..22039)
                     /locus_tag="VXM85_000895"
     CDS             complement(21116..22039)
                     /locus_tag="VXM85_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1345505.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(22053..22982)
                     /locus_tag="VXM85_000896"
     CDS             complement(22053..22982)
                     /locus_tag="VXM85_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1345506.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(23301..23651)
                     /locus_tag="VXM85_000897"
     CDS             complement(23301..23651)
                     /locus_tag="VXM85_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345507.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
BASE COUNT         7285 a         5171 c         4360 g         7220 t
ORIGIN      
        1 tatttcgact ttctttttta ctttttacat tgctatgata caatattttt aacaagattt
       61 tattatttgt taactttttt cacagaaagg agaaaatcat gcgttatgat ttcggaaagg
      121 tctataaaga aatacgtgag tcaaaggggc tgacccaaga agaggtctgt gggggtgttc
      181 tctcaagaac cagcttatca aaaatcgaaa gtggcaagac aaccccaaaa tatgaaaata
      241 tggaatttct tctccgtcaa atcaatatga gttttgaaga gtttgaatat atctgtcaac
      301 tttatcaacc aagtcaacgc acagagatta tgcaaaccta tctcaatatg cgttccatta
      361 tcgggaccag cgatcttgtc aatttatttc aaaaatgcca agactacctc aagacccacc
      421 acgatctccc tatagaagaa atcagagata tgcttgaagt tgtcatttat atccgtcaac
      481 acggggcagg ggaactatca gatcacgcag aacaggttgt caaaaaactt tggagaaaaa
      541 ttgaaaaaca agatacatgg tatgagagtg atctaaaaat cctcaatacc attcttttca
      601 gttttcccat tgaatacctc catctcatca ctggaaaaat attgcaacgc ctagaagtct
      661 ataaaaacta tcaacattta tatgacttgc gaatagcaat cctgctcaac ctttccacac
      721 tctatctata caatcaagat aaaaacatgt gtaagcaaat ctgctacact ttactggagg
      781 acgccaagaa taagaaaagc tacgataggc tggctatctg ctatgtccgc attgggattt
      841 gtacagacga ttctaaactt atccaaaaag gtttctcact cctagagctg accgaggaaa
      901 cttctatgct gtctcatctc aaaaaagaag tagagatcta ttaccaagcg aaggaaagat
      961 aaaaaagccg agggagctcc tcgacctttt catagaatcg ccgaacgatt taacgagaaa
     1021 gtatgacttt tacgtttatc ccaactcaat tatgacattt ttttcaaaag tcaatatatc
     1081 tcactttttc aacgacaaga aagaggctga taatctacca acctcttatt ctgaacccat
     1141 cactccatca ctttttagct tcattcgctt tcttagcgac tgcaatctgg tattcgactt
     1201 ggtcattccc cttaccggta caaccatgag caattgtagt cgctcctatc tgatgcgcta
     1261 tttcaaccaa ttttttagaa atcagagggc ggctcaaggc agataccaag ggatactttt
     1321 gttcataata ggcatgtgac tgaagagcca ctagcacata atctgtagca aattcgtcct
     1381 taacatcaat gacataagat tctactgccc caaccttaag agccttatca tggatgaaat
     1441 ctaagtcttt cccttcaccc acatccatac aaactgaaac aacatcatag tctttcttta
     1501 accaggtaat agcaactgat gtgtccaaac cgccagaata ggccaaaatt actttttctt
     1561 tactcatgtt cttttccttc tttctatttt tcaatggatg ctttaaaggc atcctcaatg
     1621 agtttgtcta attccccaga ctccttcaac ttttgaatgg ttttatcgac tgcctctttc
     1681 aattccttgc tatctttttt catggctacc gcgtaggaat catcttgctc tttttcaaaa
     1741 ttgaggtctg cgattgctaa atcaggatta ttttccacaa atcccttgga aacaggttct
     1801 tcaaagataa cggcatccac ttgtcctgat tttaaatctg tgattaaatt cccattttta
     1861 ggcagagata cgagggaaga attttgtagc aaatctttcg ccatcgtctc ttgaatcgaa
     1921 cctttctgcg ctccaacctt tttctgcgcc aagtcgttta cagactgata agtagtcaag
     1981 tcagattttt tgacaatgag tttatttttt gcagtatagt agggaatgga aaagtcaaac
     2041 accttgctcc gttcatctgt cttagaaaca cctgatatgg caaggtcggc ttttcctgat
     2101 tgaacactag ccagtacatt atcaaaactc atgggagata gttccaattc gacacctagt
     2161 tctgttgcga tagccttggc taattcgata tctgaaccca caatctgatt tttcccatca
     2221 accacttttt ggtattcaaa tggagcaaaa tccggattga gggctacaac caattttcct
     2281 ttggacttga tggcttcaat tccagccgat tgattgttgc tacaagcaaa tagtaggggg
     2341 agcatcacta aaccaaacat cgtcatcaac accttcttca tcttattcat aataaagcct
     2401 actttatgtt tattcctttt gtttgtataa acaatacatc tatattcatc ctttgtcaag
     2461 cctaaattga aaaaattttc aatttgaaac aaaaaaaacc tagaaatact acataaaatg
     2521 tcattcctag gcgtatttat gctatttttc tctggaaaac atgagtattc agccagtcaa
     2581 aaggagctga atgaactcat tttccctcgc ccaattcaat gacacgatga cattgttggg
     2641 ctacataagc atcgtgggtc acaataatga ctgttttccc ttctcgattc atctctaaga
     2701 gaaacttcaa gaccaaatct ctattttcag gatccagcga acctgtaggt tcatcggcta
     2761 aaatcagctg gctgggtttt aagatggctc tagcaactgc aattcgttgt tgttcgccac
     2821 cagacaactc ggagaccctt tgatgcaaag tagctgacaa acctactctc tctaaaatct
     2881 cttccacctt tttgagcttg tctttcttag gcaatttcac atatttcagc gccagcatga
     2941 gattgtactc gaccgtttca tcatcaatca gggcaaaatt ttgaaacaga taagagatat
     3001 gttcacggat tattgtttgc gacttagcag aattaaccgc tagatttgtc tgaccaaaaa
     3061 tctcataccg tccgctataa tcaccatcta tcaaacccaa taaatttaac aaggtcgact
     3121 tcccactacc actcttacca acaatagcta ccaaatcccc ctgatcaatc ctgagagata
     3181 agttatccaa aatcactttt cccccaatgg ttttggtaat atttttcaac tcaatcataa
     3241 gatgccccct ttcaataact ctactagact tcttttctcc atcctagaag ctaagcctag
     3301 cacaaatagt atatccagac atgtaaaacc tgcaaacagt agaagtggta agaacgcatg
     3361 ggcaaagaaa atcaagacta gaagagggaa actatagccc agcaagagca gaacgaggag
     3421 aggacggtag cgatcgacca gtttccaccc cataaacttc ttggtaatga tatccctgcg
     3481 cttcaataag aaagttgtta ctagtaagaa gtaggaaatc atcatgctaa ggagaccaaa
     3541 caaagcaaag agtaggttaa aattccgaac agcatctcta taagaatcca ctttctcttg
     3601 ttgaatggct tgaatagatg aaaattttaa ataatttcca tctgacaatt tctcaactaa
     3661 ctctgtaatc tctttttgat gttgaaccgt attttcaatt ttaatcggat tatttaagcc
     3721 agttgttgac agggaggctt tctcatccca catcatatca gaatcattga ccaagctaat
     3781 aattggattg gagagatttt cctttcgctt atcactatat gggaaaaatg accaatctcc
     3841 ttcataatag gcaatctcga catccatctc ctctatcgtt cgtttttgct gctcttcata
     3901 cttcatcgaa tgaaaggcaa ttaacttccc caagagctga tttttatctt cttcaccttt
     3961 cgtacttgct ggcatcaaaa taactttttt aataccggta tctggtagct tgaatccctt
     4021 gctctttaga aaattgcgat tggcatagta aacatccacc gtatctgtta actgatattg
     4081 ctgaatctgt tctgattgga caaaattttt tacaggaaga ctgctactct gcacatagcc
     4141 cgcctgcgtt ttttctacca aatcctgata aaatcgatag aaataatctg tagatttccc
     4201 tgaccctgct agctcttctt gccacagatt atcattgagt ttgaaggttt ctaaggtcag
     4261 gtaattacct tgacttaccc actgttgctg ataagcaagt tctttgtttt cttgttctaa
     4321 acttctgccc accccaatca gtaaggccgt cagtaaaata gttgtcccta ttttcatcac
     4381 ataattgaag ataagaccaa atttgaaaga tgaaaaacct ttcagcagag agctgattgt
     4441 cattttttgg attaagaggt aagtcaacca actgataaag agataaagct gcaacagcaa
     4501 aaaatgagac aaccacagca taggaaacaa atcttttggc ttataatcaa gcaagaaaaa
     4561 cacgcctaga ttgatcacaa gagccccacc taggaggagg taaaggttgc cttttacaac
     4621 atcagctaaa acagccctat cttgaaaacc aagtaatttt tgtaccccaa ctcttttcat
     4681 ctccatcatc ggttgataca ctgtcactaa cacaagaagc aaaatagcca agacaaaaac
     4741 aatggcagat aaaagcaaat ctcgatttat gacttccact gcacttttgt aggtcggctc
     4801 tagcaaggta gcctggtcta tcttgaaaaa atcgctccat ttctgtacaa tcctatcctt
     4861 gtccatctct tgtgtagaag ttatcgtata gcgaccattt aaactacgag atgtatcctt
     4921 gatataggtt tgaaaagtca taagctgaat aggtttggct tttagaaagg tcggaatcgt
     4981 accaagttta ttggaaattt ctttattact atagactcct tcaccatctg tggtaaaatc
     5041 aagagaagaa atcccaaact cttggtaggg gaaggtatct ttatcaaaaa caccagactt
     5101 gaccacctca tcaccactgt ctgttttgat gatggagact ttatactcct ttgatacatc
     5161 ctcaaaaaat cgaagaacag acgctgcagg ttcgttaata tctttcaaat acaaatccaa
     5221 agaatctaca gtctttccca tttcttgaat ccgaaccact tctcgtgtat agtttacatt
     5281 aaaaacaaaa aaagtggtac acagaagcag gagtaacata cagaaattac tgattttttt
     5341 cataaaagtg cctccactac agttttccct ctccaaatag gagagggaag aattttactc
     5401 aaatccatag aaatattcca ttcctgttgg cggaatcttg gtgagggaag ctctagccca
     5461 tgcctcaggt tttgcataat ccttagaggt tctaccccca tgtctaacag ttgctgtatg
     5521 atatcgagta gaatgtaagt aatcagaata tccaaaagtt cctgtccaac ctactccata
     5581 gttccattga ccaccctcaa cccatactgc taaagcattt gtagtagcaa gtataccaca
     5641 taccattaaa gatgcaacac taattttctc atgatttatt tctcctatta tcgagagctg
     5701 ttccgtaaaa acagctatag taatttatca acaagtatcg tagtccattg gactcacctt
     5761 ccttctatct catttcattt ttaacaacga acttactctt aaccgggaga atggtgcgct
     5821 tctgtatcag gtccttgaac aaccagagta gtattaagca cactctaact attactcaag
     5881 aatcagtcca ataaacaata tggatagcgc tttcaaattt ttaaactcct atcccatcct
     5941 tttatctata taataagtga aaatataata actgtcaagt aactgaagtg aattttataa
     6001 aaaaattaca agccaaattt gtaaagttta cactaagccg ctagggcaat cgtctatcag
     6061 aatatccgtt tatttgtcaa taatccgaga aaatcttgca acgcttagaa gtctataaaa
     6121 actatcaaca tttatatgac ttgcgaatag caatcctgct aaacctttcc acactctatc
     6181 tatacaatca agataaaaac atgtgtaagc aaatctgcta cactttactg gaggacgcca
     6241 agaataagaa aagctacgat aggctggcta tctgctatgt ccctattggg atttgtaggg
     6301 atgatgcgaa acttatccaa aaaggtttct cactcctaga gctaaccgag gaaacttcta
     6361 tgctgtctca tctcaaaaaa gaagtagaga tctattacca agcgaaggaa agataaaaaa
     6421 gccgagggat ttcctcgact ctttacatat tatttgttcg ttagttctta ataccagcca
     6481 ttgttaagcc agaagttttt agcagctgtc catgaaccgt aacgtcctgc aacgtaggca
     6541 tctgctacac gttcttggtt ttcagctgag tagtcaccgt tcaggtatga atctgttaat
     6601 tggtaacgtc cgatataacg tccatttgta gctgtatagc taccacctga ttctttttga
     6661 gcgatccatt ctttggcttc tgcttcagat ccgcttacag ttgaaacaac tgtttcactt
     6721 actactggag tttctgccac tggagctgaa acagtttcat cttgagcggc tggtgccgca
     6781 taagtagctg gcgctggtgt tgcaacaggc gctacagggc catcgataac caacttttga
     6841 tcaacataaa tcaaatgaat gttatcaatg tggttgtttt ctgccaattt ttcaactgtt
     6901 gtgttgtgag tttcagcgat ttctgaaagt gtatcacctt ctttaacagt gtaagttgat
     6961 gattcttgag cagatacaaa tgatggagca aatactgcaa acaaggcagc tactcctgca
     7021 agagttgctt taatcttttt agttattgat ttcatattcg aaaattctcc ttctttctat
     7081 agttctatca taccctttaa atattaccgt ttcttgacac ttttatgtag aaatattaca
     7141 aaattatttt ttatttccct aaataaggtg ttttttgtca taaaagatac tatttttatg
     7201 ctatttgagg tattagaaag gttttcatcc acaattaaag ccaagcccct cattctttga
     7261 tataatagag ataagaattt ttataagggg aaactgatga aatcacttcg ttttcaatct
     7321 gtctttgata tcatcggacc agttatgatt ggcccatcta gtagccatac cgctggtgct
     7381 gttcgtattg ggaagattgt ctcttccatt tttgatgata ctccgacaga agttgaattc
     7441 caacttttta actcatttgc caagacctat cgtggtcacg gaacagacct agcccttgtt
     7501 gcaggtattt taggaatgga tacagatgat cctgaaattc caaatagtct ggaaattgcc
     7561 cacaagcgtg gtatcaagat tgtctggacc attcagaaag acagcaatgc ccctcaccct
     7621 aacaccacta aaattactgt taaaaatgcc cacaagacca tcagcgtgac tggtatttct
     7681 atcggtggag gaaatattca ggtaaccgaa ctcaatggtt ttgccgtctc tctcaatatg
     7741 aatacaccga ctatcatcat cgttcatcaa gatattccag gtatgattgc ccttgttaca
     7801 gaggcgcttt cccgctatgg tatcaatatc gcccagatga atgtcactcg tgaaaaagct
     7861 ggtgaaaaag ccattatgat tatcgaagtt gacagtcgca actgtgatga ggctatcgaa
     7921 gaaattcgaa aaatccctca tctccacaat gtcaatttct ttaaatagga ggaagcatgt
     7981 tttattctat caaagaattg gtcgagcaag cagatctgga ctttcaagga aatgtcgcag
     8041 aactcatgat tacaacagag tttgaattga ccggtcgcga acgtgaagaa gtcttccttc
     8101 tcatggaacg caatctggaa gttatgaaag cctctgtcca acttggcctc aatgaaaata
     8161 aatctcgtag tggcctgaca ggtggagatg ctgccaaatt ggatcactac atcgaaaacg
     8221 gaaaaactct gtcagattat acgattctct cggctgcccg aaatgccatc gcagtcaatg
     8281 aacacaatgc caaaatgggc ttggtctgtg ccactccaac cgctggaagt gctggctgtc
     8341 tgccatccgt tctcactgct gctattgaaa aattagacct cagccacgag caacaattgg
     8401 atttcctctt tgctgctggt gcctttggac tagtcatcgc aaacaatgcc tccatctcag
     8461 gtgctgaggg tgggtgtcaa gctgaagttg gttcagcctc tgctatgagt gctgccgcct
     8521 tgactctggc tgcaggtgga acaccttatc aggccagtca agctattgcc tttgtcatta
     8581 aaaatatgct aggcctcatc tgtgaccctg ttgcaggttt ggtcgaagtt ccctgtgtca
     8641 aacgtaatgc catgggagct agctttgctt tcatcgcagc agacatggcc ttggcaggta
     8701 tcgaatctaa aatccctgtg gatgaagtga tcgatgccat gtaccaagta ggagcaagca
     8761 tgccaactgc ctttcgtgaa acagctgaag gtggactcgc taccacccct actggtcgtc
     8821 gcctccaaaa agagattttc ggagaataag cttatcaaat aggagaaacc atgacctcta
     8881 tcacagcgat ttttttcgat ttggatggaa ccctcgttga tagttctatc gggattcaca
     8941 atgcctttac ctataccttt aaagagctgg gggtgcctag ccctgatgcc aaaactattc
     9001 gtggttttat gggaccacct ctcgaaagta gttttgcgac ctgcctgtcc aaagaccaaa
     9061 tttctgaagc catgcagata tatcgttctt actataaggc aaaaggcatc tatgaagctc
     9121 aactctttcc tcagattata gacttgcttg aggagttatc gagcagttat ccactctaca
     9181 tcaccacgac aaagaataca tctactgctc aagacatggc caaaaacttg gaaatccatc
     9241 atttctttga tggtatttat ggttctagcc ctgaagcacc ccataaggca gatgtcattc
     9301 accaagcctt acagacacat caactagcac cagaacaagc catcatcatc ggagatacca
     9361 agtttgatat gctgggagct cgagaaacag gtattcagaa attggccgtc acttggggat
     9421 ttggagagca agcagatcta ctaaactatc aacctgatta tatcgctcac aaacccttag
     9481 aagttttggc gtattttcaa tagcatagac tgggtaacaa ctccatttca gaagaaaatg
     9541 aagtaatact caatgaaaat caaagagcaa actaggaaac tagctgcggg ctgctcaaaa
     9601 cactgtttta agattgtaga tggaactgac gaagtcagct caaaacactg ttttaaggtt
     9661 gtggatggaa ctgacgaagt cagctcaaaa cactgttttg aggttgcaga tagaactgac
     9721 gaagtcagct caaaacactg ttttaaggtt gtagatggaa ctgacgaagt cagtaaccat
     9781 atacggcaag gcgacgttga cgtgatttga agagattttc gaagagtata agtctctaca
     9841 taataaaacg catattacca aggttgttta acttgataat atgcgttttt cttttttatt
     9901 caatgtgggt tgtttgatta gcaaaggcga taatagcttg cttcaactca tctccactga
     9961 gagtgcggaa ctctccaatc ttttgattga tggattctaa aggcatgaca ttaaccttac
    10021 caacgaaaat cttatccata acttgattgt caacgagttc ggttgatacc aagagttctt
    10081 cgtagagttt ttcacctggg cggattccaa cttcaacgat tggaatttca ctctcagtgt
    10141 ggccacttag aagcaccatc ttcttggcca agtcataaat cttgactggt ttgcccatat
    10201 caaggataaa gacttcccca tctttggcat aagcaccagc atggataacc agacgactag
    10261 cttctggaat ggtcataaag taacgggtca tgcggaagtc tgtcaccgtt acaggcccac
    10321 cttcagcaat ctgacgttca aagactggaa tgacactacc acggctacca agaacattcc
    10381 caaaacgaac tgcacagtag gttgattggc tacgttggtt aaagccagtg acaatcaact
    10441 ccgccacgcg cttggttgct cccataacat ttggtggatt aactgcctta tctgtcgaaa
    10501 tcataaccat cttaggcacc ttggcttcat caacggctct agcaacattg taagttccac
    10561 ggatattgtt tttgaaggct tcttttggat tgcgctccat cataggaaca tgcttgtggg
    10621 ctgccgcatg ataaacaata gcaggtttgt actgctcaaa gacttgcaac aaacgatcat
    10681 agtcttgaat gtccgcaatc acaggtacat aatcaatccc ttggaactta cgaatcaatt
    10741 catgataaac aaggtagatt gagttttccc catgaccgag caagacaatg cgttcaggat
    10801 tgaagcgact aacttgacga cagatttcag aaccgattga acctccagct cctgtgacta
    10861 agatggtctt acctgtcagt tctgcaccca gacgcgattc gtcaagacgg atttcctgac
    10921 gacccaaaag gtccgtaata tcaatttttt ggaagccagt acctgcttgg tgaaggccct
    10981 gaacaacagt ttcaacctta ggcatcttgt aacatttgac acccagctta ttacacatct
    11041 gcaagatacg ctcatattct gacggatcca gcgacggaat cgcaacgatg acacgctcga
    11101 tttgatggcg tttggctaat tcaggcagat tgtcataaga gcccaaaaca ggaataccac
    11161 caagtttttg accctttttc ttagaatcct tatccaaaat accgaccagt tctaattcac
    11221 tggttggatg ttggtaacta tccataaaga gagccccacc atcaccggca ccaatcaaga
    11281 aggtccgacg gtgttctcca tcaccactac cttttttgcg tctggagtag attaactgcc
    11341 aagtaatccg tggcaataaa atcaagaagg tactcaacaa gataaagaga atgatgaaac
    11401 ggatggagaa gagtggcaag aaggcataac agatactata tgacaagaca ctgctagcag
    11461 tcacaccaaa aaagattttc atgaaatccg taatcttgct gtaacgacta atgctcgcgt
    11521 tcaaccccca aaaaccaatc atcaattgat agaacaagaa ggccaaactc gtatagataa
    11581 tgtagtcaac aggtgctgga ttaatcagcc cataaaataa aatataagat acaattatgg
    11641 aaaccaccat actgaaaata tcaaatattc cccagaaaac ctgcttttgt tgtttattta
    11701 aaatttccac cagatcaatc acataatctg ttagtttttt attcatagaa atctactcct
    11761 ccttaaaaga gttttactat attgttatta tagcatattt tgatttattt ggcttatttt
    11821 atccaaataa attattgttc ccttgtactc cttcttccat ttaaatcctc taattaaacc
    11881 tacaatcgtc ccaaggccat aagcaaagtg aatggaaaat aaaagaaagg gcatcacaat
    11941 tagaaatcca tttttatgtt ttaataaagt cagcaaagtg agtaatgaca aaagtagaaa
    12001 ataggcacct aatagtaaag ttatgaatac gaatgtgatc ggtaacaatg ctagactaaa
    12061 cacaagactc aaaacaaata aacaaggaac atagtgaaat aatgataaac acttaaactg
    12121 aacatgactt gtcaagccaa tccacaaacc atttgaatac ttttgatgca gcattttctt
    12181 gaatgttggt cgaatatact gataagatag aatacttggg ctatagcgga ttttataacc
    12241 atgttctcga attctataat gaatatcatt atcttcagtt cggccaagtt gctcatttac
    12301 taaaccaacc ttctggaaaa cctctcgttt atacatccca tgaaaaatag aagaaacata
    12361 tctatcctcg gaactatttc gataattggc aatgctactg ccaaacatat tttcctcaac
    12421 aagatgcaag gtctctgccc attttccttt tccttcgaca atcgtcggtc taggcccccc
    12481 acagacaaat tcaccttgtt gaataatagc cacattgtta attacaaaac tctcagtaac
    12541 ttttgaatga gcatcaattt ttaaaataag gtcccctaca gaatgtttaa ctcccaggtt
    12601 aaaaccacta gcttgatttt tcttaggatt gttatacaat ctaattgagt taaactctgt
    12661 atcttccttt ataaattgct gaatgatagc tgtggtccca tctgtggaca tagcatttat
    12721 aaatagaatt tcaatatcct ctttaggata ggtttgattt tttaagtctt caattagacc
    12781 aggcagatat ttttcttcat tgtatgcact gattacaatg gatacgagaa gactttcaac
    12841 attttgagtc actactactc tcctgaattt ttccttccac cattctatca taattttcaa
    12901 gactttccca tttttatctt gaaagccaga aaccttactt gacattatag tgaaaaagcc
    12961 ttaaaataag ctgttattaa aatcagctag aagaggtatt atatgaaaaa gcaatcactc
    13021 ttttttgttc caggtattat cctgattggt gtttccttgc gaactccttt tactgtttta
    13081 cccattattt tgggaaatat ttcgcaaggt ctggaggtag aagttagttc gcttggtgtc
    13141 ttgaccagcc tgcctctcct tatgtttacc ctcttttcac cattttctac ccaactggct
    13201 cagaaaatcg gcttggagca tctcttcacc tacagcctct tcttcttgac catcggctca
    13261 cttattcgac taatcaatct gcccctgctc tatctaggaa ccttgatggt tggggcaagt
    13321 gtcgcagtca tcaatgtcct gcttcctagt cttatccaag ccaatcaacc aaagaaaatt
    13381 ggttttctga ccaccttata tgtaacgtct atggggattg caacggctct ggcttcctat
    13441 ctagctgtgc ccattacaca agccagttct tggaaaggac ttatccttct cctcacgtta
    13501 ctctgtctag caactttttt ggtctggctc ccaaatcacc gctataatca tagactagct
    13561 ccacaaacca aacaaaaaag tcaaataaag gtcatgcgta ataaacaggt ttgggcaatt
    13621 attatctttt caggttttca atccttgatc ttttacaccg tcatgacctg gttacctacc
    13681 atgtctatcc atgcaggtct atccagtcac gaagctggct tgctgacttc tatcttatct
    13741 ctgattagca ttcctttttc aatgaccatc ccaagcctga caaccagttt atctactcgc
    13801 aaccgtcagc tcatgctcac tctggtttca ctagctggtg tggtcggcat ttccatgctc
    13861 tttttcccaa tcaataattt catttactgg cttgccatcc atctcctcat cggaaccgca
    13921 accagtgccc tcttccctta tctcatggtc aacttttcac tcaagacaag cgcccctgaa
    13981 aagacagccc aattgtccgg cctatctcaa acaggaggct atatcctagc agcctttggg
    14041 ccaaccctct ttggttacag ttttgacctg ttccactctt gggtaccatc tgtagctgcc
    14101 ctcttgctca tcgatatcct gatgactgtg gccctcttta cagtggacag agcggataag
    14161 atcctttaaa cttctagttt tagctagaag ttttttatat ataaaaattt tacacatttc
    14221 tcttgacagg gctttctttt agatgtacaa tgtatgtgta gaaaaattat atataaaaat
    14281 cttacacatt agaaaaggag gtttcccatg tactttccaa catcctctgc cttgattgaa
    14341 tttctcatct tggctgtact ggagcagggt gattcttatg gttatgagat tagccaaacc
    14401 attaagctga tcgctaatat caaagaatcc acactctatc ccattctcaa aaaattggaa
    14461 ggcaatagct ttctgacaac ctattctaga gagttccaag gtcgcatgcg caaatactac
    14521 tccttgacaa acggtggtat agagcatctc ttgaccctaa aagatgaatg ggcactctat
    14581 acagacacca tcaatggcat catagaaggg agtatccgcc atgacaagaa ctgaatacct
    14641 gactcagcta gaactctatc tcaagaaact acctgaagct gaccgtatcg aagccatgga
    14701 ctatttcaga gagctctttg acgatgctgg agtcgaagga gaagaagaac tcatcgctag
    14761 tttgggaact cccaaagaag cggcccacga agttctatcc aatcttctcg ataaaaaaat
    14821 caatgaagca cccgctcaaa aaaataaccg acaaatttta catatcgcct tgttagccct
    14881 ccttgcagca cctatcggca ttcctctggg aatcgccatc ctcgtgaccc tgttcgcaat
    14941 tcttgtagcc gctttgactg tcattctggc tttctttgca gtttccatac tgggtatcat
    15001 cggcggattc ctatttttag ttgaaagttt cactgtcctc gcccaagcca aatcagcctt
    15061 tatcttgatt tttggttctg gtttactggc tatcggtgct tcttcgctag ttttacttgg
    15121 catttcctat gcaactcgct tcttcggtct actcattgtt cgtctggtac aatttgttct
    15181 taaaaaagga aagagaggta atcagcatgc gtaaatggac aaaaggattt ctcatctttg
    15241 gtgtggtgac taccgttatc ggctttatcc tgctttttgt aggtatccaa tctgacggga
    15301 ttaagagcct actttccatg tccaaagaac ctgtctatga tagccgtacg gaaaagctaa
    15361 cctttggcaa ggaagtcgaa aacctagaaa ttactctcca ccaacacacg ctcaccatca
    15421 cagactcttt cgatgatcaa atccacattt cttaccatcc atctctttct gctcaccatg
    15481 atcttatcac caatcagaac gatagaactc tgagtctcac tgataagaaa ctgtctgaaa
    15541 ctccgtttct ctcttctgga attggtggga ttcttcatat cgcaagtagc tactctagtc
    15601 gttttgaaga agttattctc cgactaccaa aagggagaac tctaaaaggg atcaacatct
    15661 cagccaatcg cggacaaacc accatcataa atgctagcct tgaaaatgcg accctcaata
    15721 caaacagcta tatcctccga attgaaggaa gtcgtatcaa aaacagtaaa ctcacaacgc
    15781 ccaatatcgt taatatcttt gatacagttc ttacagatag tcagctagag tcaacagaga
    15841 atcacttcca cgctgaaaat atccaagtcc atggcaaggt tgaactgact gccaaagatt
    15901 atctcagaat catcctagac cagaaagaaa gccaacgaat taactgggac atctcaagca
    15961 actatggttc tatcttccaa ttcacaagag aaaagcctga atcaagaggt acggaattaa
    16021 gcaaccctta caaaactgaa aaaaccgatg tcaaggatca actcattgcg agatctgatg
    16081 ataatattga tctaatatcc acaccaagca gacgttgaca aaaacgctta catttgctac
    16141 tatggaaaca tatttatcta actaaaaagg aggttctacc atgaaacaag aatggtttga
    16201 aagtaatgat tttgtaaaaa caacaagcaa gaacaagcct gaagagcaag ctcaagaggt
    16261 tgcagacaag gctgaagaaa cgatagccaa tctcgataca ccaattggaa aaaatactca
    16321 agtagaggag gaagtctctc aagctgaagt cgaactggaa agccagcaag aagagaaaat
    16381 tgaaactcct gaaggcggtg aagcgagaac agaaatagaa gaaaagaagg catctaattc
    16441 tactgaagaa gagccagacc tttctaaaga aacagaaaaa gtcactatag ctgaagagag
    16501 ccaagaagct cttcctcagc aaaaagcaac cacgaaagag ccacttctta tcagtaaatc
    16561 tttaaaaagt ccttatatcc ccgaccaagc tccaaaatct agggataaat ggaaagagca
    16621 agtgcttgat ttttggtctt ggctagtaga agcgatcaaa tctcctacaa gtaagttgga
    16681 aacaagtatc acacacagtt acacagcctt tctcttgctc attctgtttt ctgcatcttc
    16741 ctttttcttt agtatctatc acatcaaaca tgcttactat ggacatatag caagcattaa
    16801 cagtcgcttc cctgagcagc tagctccttt aactcttttt tctatcatct ctatcctagt
    16861 agcgacaaca ctcttcttct tttcattcct cttgggtagt ttcgttgtga gacgatttat
    16921 ccaccaggaa aaggactgga cgctagacaa ggttctccaa caatatagtc aactcttggc
    16981 aattccaatc ttcctcactg ctattgctag tttctttgct ttctttgata gcctacgatt
    17041 tacagctctc ttgtgtgtga ttagcattgg aatcattctg cttgccagtc tccatatcat
    17101 tacaagacca agtcaagcaa gtgaaaccga ctccttctat caattattct tgtctgtcct
    17161 tgtgaacgga gttattatcc tcctcttctt tgtagctgaa gtggcactga ttggagatta
    17221 tcttcgtatc ttggcctttc tttaaaattc aatcctgtca tccttggcag gattttttct
    17281 atgtcaaaaa agcaagtcga ttgacctgct tctgctttac ttttcttgtg ctaatgtttt
    17341 aaaatctttg tctaatatgg gttgcacttc tatttgattg gcgtctagtt ggtggtaaaa
    17401 agatgggggt aatacctcta agaattttgc atagtccaag cgagctatgg attcatagtc
    17461 ttctggttta gagccgtctc ctgtgatttg aaccaagtca atctcccact ggacttcctt
    17521 atccaccaat tgctcaatat aggctgcagg aacaaacggt tctctcggta aaactgtctt
    17581 gactccttga gctaaatggt aaacgccatg cacttttcct tctccatcct gatagaagga
    17641 aactcttgca aagtaggcat ctgtctcttg aaccgcacgc acacgcgctt caaactgagc
    17701 caagccatct gcctctaaaa agcttttcaa gttctcacga ctaaagaaaa taggattaaa
    17761 aattccttgg caaatcgtaa aacgagctac agggtctgct agataggctt gaatacttgc
    17821 ttggaaataa gcttcaaaat caaaaccatc tagcccttca atatcagtcc ctgtataagc
    17881 aagcaaagca tctaaaaatg acttgataat ctgccagtct gtctttgtta gtaggtcagg
    17941 aagatcgaca cggtaagcct tttgaccatc agcataactg accttaaaga gaaattgtga
    18001 ttgccccact atcgcacact caatatactc gagacgatta agaggctgac gaagatagac
    18061 tacatcatag ctatgtgact ccaaaccgtc tagcaaggcc aagatcgact tggcattcaa
    18121 aatctcttgt tctcctaaaa tgctctgttt atttggaata aaaaatgttt ttgccataac
    18181 tggactcctt tgaaatcgtt tacatacagt ataccttatt ttcctgaaag atacaaaaac
    18241 aaactttaga tttttgagta caaagcatag aaaaacagcc cctgaggact gtttgatctt
    18301 atacagtagc tgcctgatcc aagctttcac cgatagcggc taggcgctca atcacttcag
    18361 cttgtgtcaa ttcatttttt gaaacatagc ggttacgtgg gtgaacacgg cactcgtgtg
    18421 agcatccacg aaggtacttg tcttcatttt cttctgatgt caagatacga cggttacaga
    18481 agggatttcc acagttgaca taacgttcac atggtgttcc atcaaaccag tctttcccta
    18541 cgatggttgg gttgacatgg ttgacatcaa cggcaatacg ctcgtcaaag acgtacattt
    18601 tcccatccca aagctcacct tgaacttctg ggtctttacc gtaagttgcg attcctccgt
    18661 gcaattggcc gacatctttg tagccttcac ggaccatcca gcctgagaat ttctcacagc
    18721 gaacgccacc tgtacagtaa accacgacac gcttgtccat gaatttttcc ttgttatcac
    18781 ggacccattg tggtaactca cggaagttgc gaatatctgg gcgaatagcc ccacggaaat
    18841 gtcctaggtc gtactcataa tcgttacgtg tgtcaaggac aacggtatct ttatcaagaa
    18901 gcgcttcttt gaactctttt ggagacaagt aagcacctgt tgtttcaagt gggttgatgt
    18961 cattgtcaaa gtcgttgtct tccaaaccaa ggtggacaat ttctttcttg tagcgaacaa
    19021 acatcttctt gaaggcttgt tcattttctt cgtcaatctt gaaccagagt tcttccattc
    19081 ctggaaggct gtgaacgtag tccatgtatt tttgagttgt ttcatagtca cctgaaactg
    19141 ttccgttaat tccctcgtca gcgactagga tacggccttt aaggccgatt gatttacaga
    19201 aagccaagtg gtctgcagca aattgctctg cattttcaat tggagtataa aggtagtaaa
    19261 gtaagacacg aatatctttt gccataagat ttgttctctt ttctattctt aaattttcta
    19321 aattttttat ttatctacac tagtatacct gcttgagaaa acgaatgcaa tttatttgac
    19381 tggaaattgt agagggttct cgaaatttcc tgaatggtta aaataaggac aagagaaaac
    19441 atggatatct atatccttgt gccaaaaaaa ccactgccct ccccagacca acctgaggaa
    19501 agcggtgatt cttattttag gagttaggaa tgaatacacg aaatcaattt agctgattat
    19561 tttttgtttt tcaagaattc atcgtattgt ttttgcattt cgttcaatac tttttcgtag
    19621 gcaccttcag atttcaattt ttccatcaat tctggaatcg ctttatctgg gtctacagta
    19681 ccagtgttga tagctgtatc aaattgttgc attgtgttag caatagctga gatttcagat
    19741 ttcacattgt cagtattgaa gataaatcca agcgctggag attctttagc ttctgccaat
    19801 tctttcttag aattttcgat ttgttggtct gtaacgtttt cgttgatgta aaggatccag
    19861 ttgttaccag tgttccatcc acccatgtga gtgtttcctt tgtagccatc aagaacgcga
    19921 acacggtttt ctttaccttc aattttttcc cagttcttgc cttctggacc gtaaacaaga
    19981 ccgttcaaaa gttctgggtt cgtattcaag aggttcaaga tttccattga tttttctttg
    20041 ttcttagagt tgtttgagat gacaaagtta gcaacttgtg ttgtttggtt tttcttgatg
    20101 aagttagtga ttggtttgat ttggatatct ttgttggcaa cacgtgaaag caagctgtta
    20161 ccgtagtcag ctggtcctac tgtttcttca cgaacgaacc aagtatcttg ttgaaggtca
    20221 aatgaagtgt cacttgttgc gacgtctttt ggaatgtagc cagcttcata gaatttgtga
    20281 agagtcttca agtgttcttt gaaacgaggc acttcgtaac ggtttacaac tttagtagta
    20341 tcgccttcaa ggtcaataac gaatggaagc ccgtttgcta ctgggtagtc aaagttatca
    20401 gatgggatga aaactttacc aatagcaaat ggtactacgt ctggagcttt ttctttgatt
    20461 tgtttcaaga ctggctcaag agtttcgtaa gaagtaacac ctgaaatatc gataccatat
    20521 ttggcaagga gagttccgtt gaaggcaaag ttttgagatg atgctacgtt ggctgcaact
    20581 ggtacagcgt aaatcttacc atttacagta ttacccttga tataagctgg atcaagtgcc
    20641 ttgtaaaggt ctttaccttc ttttttgtac aattctgtca agtcagcata agcacctttt
    20701 tgagcattta caatatagtt atctgcaaag gcgatatcat agttttcacc agatgatgtg
    20761 ataactgaca ttttcttacc atagtcaccc cagccaaggt attggatatc caatttggca
    20821 ccaacttttt cttcgatgat tttgttggca tttgctaaca attcatccaa gttatctggt
    20881 ttgtcaccga tttggtacat tttgataaca ggtttgtcac ctgaatcagc agcttttttg
    20941 ctgttacctg tcaaatttcc acaagcagca agacctgcag ccaaagcgac tacactagca
    21001 gatgcaaaag catatttttt ccagtttttc atgataaaaa ctcctttttt tatttttaaa
    21061 cttataaaca atgtaatgat cttaacttca cgcaagggaa gtttggaaac aagtgctatt
    21121 ctttcacacc accgatagtc aaacctttta caaagtagcg ttggaagaat ggatacaaaa
    21181 ttgcgattgg aagggttgct accacaacca tggccatacg acctgtttct ttcggtagag
    21241 caactcccag ttgaccagtc aagccgaccg ctttggcaat gtagtccata ttttgttgga
    21301 tttgcatgag caaatattgc aatggataca agttgtcact cttgatgtaa agaagggcgt
    21361 tgaaccagtc attccagaaa ccaagagctg tcaaaagcgt gatggttgcg atacctggta
    21421 gtgacaatgg caaacagatt tggaagaaaa tccgggcctc actggcacca tcgatacgag
    21481 ccgattcgag aatggcttct ggaatggtct tcttgaagaa ggaacgcatc aagatgatat
    21541 tgaatggtga gagaagcatt ggaacaatca aggcccaaac agtgtcacca agctgaagta
    21601 cacgggtcac catgatataa cctggtacca aaccagcgtt gaacaacata ctgagaagga
    21661 cgaagatggt aaaaaatctg cgatacttaa aggttgtccg tgaaatagcg taggcatagg
    21721 ttgttgtgat aaagacattt gtcaatgtcc caactacggt tacaaagaca gagatgaaga
    21781 gggcttgtag gattttatcc ttaaactgtg ccaaaaactc aaaaccgtct aagccaaatt
    21841 gggatgggaa gaagctatag ccgtattgga ggaggctttt ctcgtctgtc actgaaataa
    21901 tgataacgaa tacaaaaggt aggatacaag agagggcaat caaacccgaa atgatactga
    21961 agaagatatc tgctttctta ctgaaggagt gaatgccgac attatcaatt ttttcttttt
    22021 taattttctt ttttgccata ttctcctcct ttctagaaca aagctgagtt tggatcgact
    22081 cgtcttgcaa gcaagtttga taggataacc agaatcaaac caacaacgga ttggtaaaga
    22141 ccggctgctg cagccatacc gatatctgct gtctgagtca aaccattaaa aacatatacg
    22201 tccaaaacgt tggttacatt gtaaagctga ccagcattgt gtgggatttg atagaagaga
    22261 ccgaagtctg cgcggaagat atttccgact gcaaggatgg tcaatacagt tacaagcgga
    22321 gtcaactgag gaatggttac gttgcgaata cgttgccact tgctagctcc gtccactgtc
    22381 gctgcttcgt agtaggttgg atcaattccc atgatcgtcg catagtacat gacactgcta
    22441 tatccaaaac ctttccaaat acctaggaaa agtaggagat agggccagat gcccaggtca
    22501 gcgtagaaat tgacttcttt gagaccaaga ctttccaata gatgattgaa caccccttta
    22561 tcaatattta ggaaggcatc tgtaaagaaa ctgatgataa cccaagacaa gaagtaaggg
    22621 aacaacatag aagtttggaa aatcttcacc attctcttag aacggagctc gctgaggata
    22681 atggcaatcc ctacagatac aactaaacct agaaagataa agccaagatt gtagaggaca
    22741 gtatttcgtg tgataataaa ggcgtctctt gaactaaata agaatctgaa attatcgagt
    22801 ccgacccatt tactatttat gatactatct atgaaaccat tactggtcat gtggtagtct
    22861 ttgaaggcaa ccacgttccc aaatactgga atgtaaaaga atagaatcaa ccagagtgcc
    22921 cctggcaaaa ccatcaagag aaagatccag ttgtctctca atgtttttga aaactttttc
    22981 ataatttcct ccctttttat tttgatatcc atctaaaaat tcttttttag acttttgata
    23041 acgattacat tattagtata ctcctatttg caggttaggt taaactccta attatagaaa
    23101 aaattccaca aattatgtag cagatttaaa actttatcac cactatcaaa caaatgtcct
    23161 aaatcaattg tttattttat ctctattagc ccagtgatgg cgtcactctg ttataagcat
    23221 ccaacaacgg ggtatactga aaaatctcca gactagggaa ctcagcgata gttcctaatc
    23281 tggagatttt taatatgtta ttaggcgttt gctttcaact tagcaataac ctctttaaga
    23341 ttatcaatca actctgcttc agtatgctca gagccttttt catctgccaa gaacaaaact
    23401 gctttttgaa gttcttcttg agagttttca aggacatcct tatctgctgt ttctttgccc
    23461 gctgtttgtc ctccatcctt agttgctgac tggctttcct taatggactc tagggaagca
    23521 atggcatctt tgactgtttg caagatatca cgtaaacctt gctctgtcaa actatcatct
    23581 gtaagagctt tcttagcctc tgccaaaacc agacgtgctg aatctgtggt aggattcgat
    23641 acacctgtca atgatctcaa aagattttct aaggtttgag tctgcttact aatactagac
    23701 taaaatcaaa aagcattaca taacagtgat atgaaatcaa ctaaagaaga aatccaaacc
    23761 atcaaaacac ttttaaaaga ctctcgtaca gctaaatatc ataaacgcct tcaaatcgtt
    23821 ctatagtaaa atgaaacaag aacaggacaa atcgatcagg acagtcaaat cgatttctaa
    23881 caatgtttta gaagtagagg tgtactattc tagtttcaat ccattatatt tcgtctgatg
    23941 ggcaaatctt ataaagagat tatagagctt ttatagtgat ttgaaataag atgtgaacaa
    24001 ctctatcagg aaagtcaaat taatttatag aaatat
//
LOCUS       DAVLRT010000022        23970 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLRT010000022 DAVLRT010000000
VERSION     DAVLRT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23970
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.022"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(39..3215)
                     /gene="carB"
                     /locus_tag="VXM85_000898"
     CDS             complement(39..3215)
                     /gene="carB"
                     /locus_tag="VXM85_000898"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1345508.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3528..4607)
                     /gene="carA"
                     /locus_tag="VXM85_000899"
     CDS             complement(3528..4607)
                     /gene="carA"
                     /locus_tag="VXM85_000899"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1345509.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4657..5580)
                     /locus_tag="VXM85_000900"
     CDS             complement(4657..5580)
                     /locus_tag="VXM85_000900"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1345510.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5599..6120)
                     /gene="pyrR"
                     /locus_tag="VXM85_000901"
     CDS             complement(5599..6120)
                     /gene="pyrR"
                     /locus_tag="VXM85_000901"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1345511.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6331..6960)
                     /gene="nth"
                     /locus_tag="VXM85_000902"
     CDS             complement(6331..6960)
                     /gene="nth"
                     /locus_tag="VXM85_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1345512.1"
                     /translation="MVLSKKRARKVLEEIITLFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6960..7502)
                     /locus_tag="VXM85_000903"
     CDS             complement(6960..7502)
                     /locus_tag="VXM85_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1345513.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLCARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            7672..7989
                     /locus_tag="VXM85_000904"
     CDS             7672..7989
                     /locus_tag="VXM85_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1345514.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FEF"
     gene            8228..9787
                     /locus_tag="VXM85_000905"
     CDS             8228..9787
                     /locus_tag="VXM85_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1345515.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLSKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIHSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9831..10730)
                     /gene="htpX"
                     /locus_tag="VXM85_000906"
     CDS             complement(9831..10730)
                     /gene="htpX"
                     /locus_tag="VXM85_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1345516.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(10732..11292)
                     /locus_tag="VXM85_000907"
     CDS             complement(10732..11292)
                     /locus_tag="VXM85_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1345517.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11386..12099
                     /gene="rsmG"
                     /locus_tag="VXM85_000908"
     CDS             11386..12099
                     /gene="rsmG"
                     /locus_tag="VXM85_000908"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1345518.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12353..13636
                     /locus_tag="VXM85_000909"
     CDS             12353..13636
                     /locus_tag="VXM85_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1345519.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMLATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(13831..15402)
                     /gene="ffh"
                     /locus_tag="VXM85_000910"
     CDS             complement(13831..15402)
                     /gene="ffh"
                     /locus_tag="VXM85_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1345520.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKVLAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15414..15746)
                     /locus_tag="VXM85_000911"
     CDS             complement(15414..15746)
                     /locus_tag="VXM85_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1345521.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(15837..16214)
                     /locus_tag="VXM85_000912"
     CDS             complement(15837..16214)
                     /locus_tag="VXM85_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1345522.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            16286..17590
                     /locus_tag="VXM85_000913"
     CDS             16286..17590
                     /locus_tag="VXM85_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1345523.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            17603..18409
                     /gene="yidA"
                     /locus_tag="VXM85_000914"
     CDS             17603..18409
                     /gene="yidA"
                     /locus_tag="VXM85_000914"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1345524.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETDTGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTLVL"
     gene            complement(18464..18535)
                     /locus_tag="VXM85_000915"
     tRNA            complement(18464..18535)
                     /locus_tag="VXM85_000915"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18500..18502),aa:Arg,seq:cct)
     gene            complement(18577..18924)
                     /gene="rplS"
                     /locus_tag="VXM85_000916"
     CDS             complement(18577..18924)
                     /gene="rplS"
                     /locus_tag="VXM85_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1345525.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(19043..19372)
                     /gene="crcB"
                     /locus_tag="VXM85_000917"
     CDS             complement(19043..19372)
                     /gene="crcB"
                     /locus_tag="VXM85_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1345526.1"
                     /translation="MVIVYLAIACGFGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(19366..19740)
                     /gene="crcB"
                     /locus_tag="VXM85_000918"
     CDS             complement(19366..19740)
                     /gene="crcB"
                     /locus_tag="VXM85_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1345527.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(19737..20003)
                     /locus_tag="VXM85_000919"
     CDS             complement(19737..20003)
                     /locus_tag="VXM85_000919"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1345528.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(20119..20562)
                     /locus_tag="VXM85_000920"
     CDS             complement(20119..20562)
                     /locus_tag="VXM85_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1345529.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            20666..21601
                     /locus_tag="VXM85_000921"
     CDS             20666..21601
                     /locus_tag="VXM85_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1345530.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            21655..21939
                     /locus_tag="VXM85_000922"
     CDS             21655..21939
                     /locus_tag="VXM85_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1345531.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(21996..22574)
                     /locus_tag="VXM85_000923"
     CDS             complement(21996..22574)
                     /locus_tag="VXM85_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEV1345532.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKT
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSIAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(22549..23562)
                     /locus_tag="VXM85_000924"
                     /pseudo
     CDS             complement(22549..23562)
                     /locus_tag="VXM85_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            complement(23565..23970)
                     /locus_tag="VXM85_000925"
                     /pseudo
     CDS             complement(23565..23970)
                     /locus_tag="VXM85_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         7025 a         5408 c         4585 g         6952 t
ORIGIN      
        1 ctgttctaaa ttcagtctgc tatatcttat ttttctattt aaatcgcttc tgtaacaaag
       61 ctacgacttt caagtacctt aagcatggca ttagctgtat ctagcgctgt gaagaggggc
      121 accccgtgtt caatggctga acgacgaatt tgctcaccat cttcgtcagc agttcgtttt
      181 gttcctactg tgttaatgat agcttgaatt cttcctttgc gtacaaaact tgggatatcc
      241 ttatcgtcat caccaatctt accaacaggt tgggcttgca agccatggct agcaaagaag
      301 gctgctgtcc cttctgtcgc aaggattcca taaccaatgt tttggaaacg acgagccaag
      361 ttcaaggctt cttctttggc atcatcagcg atggtaaaga cgacattacc aaaagttggc
      421 aagtgtagat aagaagcttc aaaggcttta tagagagctt tttccaaagt agcatcagaa
      481 cccataactt cacctgttga cttcatttca ggaccgagca agctgtctac cttagctagt
      541 ttggtaaagg agaagacagg tgccttgata tgaacacggg tgctttcagg gtaaagtcca
      601 ttttggtagc caagttctga taaactttga ccaagaatga gcttggtcgc tacttgagcc
      661 ataggaatat tggttacctt agataggaat ggaacagtac ggctggcacg tggattgacc
      721 tcaataacgt agactttttc atccttgata acaaactgga tgttcatcat tccaaggcag
      781 tgaagaccga ttgctaagcg tttggtgtag tctgcgatgg tctcctgaac cttttgcgac
      841 aaggtttgtg gtgggtaaac agccattgag tcacctgagt ggacaccagc acgttcgata
      901 tgctccatga taccaggaat gagtacatct ttaccatctg aaatggcatc aacttcgcac
      961 tcttgcccaa cgatataaga gtcgacaaga actgggtgat ctggactagc cttaacagca
     1021 gttcgcatgt aagaacgaag gtcttcttcg ttttcaacga tttccatggc acgtccacca
     1081 agtacataag atgggcggac aagaactggg aagccaatct tacgagctgc aagagctgct
     1141 tcttcttcat tggtagccgt ttgtcctggt ggctgtggaa tatccaattc tttgagagct
     1201 tgctcgaaga ggtcacggtc ttcggcacgg tctaggtcag caacctgtgt accaaggatg
     1261 gtcacacctg cttttgccaa tggctccgca aggttgatgg ctgtttgacc accgaactga
     1321 acgataactc cctttggttg ttccaagtca atgacgttca taacatcttc gaatgtcaat
     1381 ggctcaaagt aaagcttatc tgatacagag aagtctgttg aaacggtctc tgggtttgag
     1441 ttcatgatga tagcttcata accagctgcc tggatagcct taacagagtg aacggttgcg
     1501 tagtcaaact caaccccttg accgatacgg attggacctg aacctaggac aagtacagat
     1561 tctttatcag atctgataga ttcattttcc caaccatagg ttgaatagaa atatggcgtt
     1621 tcggagtcga actctgccgc acaagtgtct accatcttat aaactggaac aatcttgttt
     1681 tccaagcgaa gttggcgaac tttatcatca gtcgttcccc agagttcagc aatcttacgg
     1741 tctgaaaaac cattaagttt ggctgttttc aaaacttcta gatcttgtgg atgagcaccc
     1801 aattcttgct caatttcaaa gatatgcaag agtttatcaa gatagaagat atcaattttt
     1861 gtaagctctg caatttcttc aggtgtgtag ccacgacgaa ttgcttctga tacgtagaag
     1921 agacggtcat cttgggcttt gacaaccttt tcaatcaagg catcatcaga aactgctgca
     1981 agttcaggta tttcattgtg gtgcacccca atttcaaggg agcggcaggc cttgagaaga
     2041 gattcctcga tgttacgacc gattgccatg acttctccag tcgccttcat ttgtgtaccg
     2101 agacggcgtt cacccttttc aaacttgtca aatgggaaac gtggaatctt agcaactacg
     2161 tagtcaaggg ctggttcaaa catggcatag gttgaacctg taactgggtt tataacctca
     2221 tccaaggtca aacctactgc aatcttggca gccaacttag caatcggata tcctgtcgct
     2281 ttagaagcaa gggctgacga acgtgataca cgagggttta cttcgataac ataatacttg
     2341 aagctgttag gatcaagagc tagctgaaca ttacatccac cttcaatctt gagggcacga
     2401 ataatgctca agctcgcatc acgaagcatt tggttttcat agtctgacat ggtttgcgca
     2461 ggggcaaata caatggaatc ccctgtgtga atcccaactg ggtcaaagtt ttccatgtta
     2521 caaacaacca aggcattgtc agctgagtca cgcatcactt cgtattcaat ttccttgaaa
     2581 ccggcaatcg aacgctcaat caaacattgg gtaacaggtg acaatttcaa accattttca
     2641 gtgatttcac gcaattcttt ctcgttggca cacataccac caccagtacc accaagggta
     2701 aaggctggac gaacgatgac tgggtagcca attgtcgctg caaaggcaac tgcttcttct
     2761 actgtgttaa caatttcaga ttctggaatg ggttgttcaa gctcttccat caattgttta
     2821 aagaggtcac ggtcctccgc ttggtcaatg gcagataatt tggtacccag aagttcaacg
     2881 ccaagctcgt ctaggatacc atttttagat aattccatgg ccatgttgag acctgtctga
     2941 ccaccgagtg ttggtagcaa ggcatctgga cgttccttac gaagaatacg tgtcacaaac
     3001 tcaagtgtaa tcggttcaat gtaaaccttg tcagcaattt ccttgtccgt catgatggtt
     3061 gcaggatttg agttaaccaa aacaacctca taaccttcct ctttcaacga caagcaagcc
     3121 tgagtcccag cgtagtcaaa ctcagcagcc tggccaataa taatcggacc agaaccaatc
     3181 accataattt tttgaatatc agtacgttta ggcatatata agatattaag ggcgtcaagc
     3241 ggacaaagct aaaataggag ttatgacgaa gaactgtcag ttctaggaat aactatcttt
     3301 ttagcaccgt ccgtagcccg tattcagttc agcaaatacg gagcaccctt ctcctttcta
     3361 ttcgtcgcct ctcagggcga cattaaataa gatacaaagg acgaatagaa agcgattgaa
     3421 ttttagaaaa tcaaggaagg attgacaatc caagttgatt tctctacatt ctgagctttc
     3481 cgtccgtgtt cagttacata aattctccga cgagctttta ctcgttctta gtttgattgt
     3541 ttaaaaactt ccatcatctc gataaactcg tcaaataggt agctagcgtc gtgtggccca
     3601 ggagctgcat ctgggtggta ttgaacagag aaagcaggtt ggtatctgtg gcgcacacct
     3661 tccactgact tgtcattgat ttcttcgtgg gtaataatca agtgctctgg caaatcctcg
     3721 cggctgactg cataaccatg gttctggctg gtgaagtcta ctcgtcctgt tgcgatttca
     3781 cgtaccgcat ggttgaatcc acggtggcca aacttcatct tataggtctt agccccgttt
     3841 gccattgcaa agagttggtg tcccatacaa ataccaaaga ttggaatttt tccttgtaca
     3901 ccgcgaatca tgtcgagtgc ttgtggaacg tcttctgggt tacctggacc atttgacaac
     3961 ataaccccat caggattgag atggagaatt tcttcagccg ttgtcgaata aggaacaact
     4021 gtcacgttac agttgcgttt agaaagttca cgtaggattg agtgcttgag accaaagtcc
     4081 actagcacca cgctcaaacc aactcctgga gctggataag acgttttagt agaaacctgt
     4141 ttgatattgt ctgtcggtaa aactgttgct tggagctggt ccgtcacatg gtccatactg
     4201 tccccaacat gggtcaaggt tgcacgcata gtaccatgct tacggataat cttggtaaga
     4261 gcacgcgtat caattcctga aatccctgga atttttttgg ctttcaaaaa ttcatccaag
     4321 gtcatttggt tgcgccagtt gctagctcta cgcgcttctt caaaaacaac gactccctta
     4381 caagttggaa taatggattc ataatcatca cgattaatac cataatttcc taccaaagga
     4441 taagtaaagg tcaagatttg tccattataa gactggtctg taatggattc ttggtagccg
     4501 gtcatccctg tattaaagac gatttcgcct gttacatcaa tatctgctcc gaaggccttg
     4561 ccttcaaaaa ctgtgccatc ttctaatact agaattcttt ttgtcatatt ttcacctctc
     4621 gtggacgctc actggcgtct tttaacgtct tgtgttttag ttggcgtttc tactcgctag
     4681 tacggattct aagattgcca ttcgaacaaa gacaccattg gtcatttgtt ggacaatccg
     4741 tgattttggt gcttcaacca agtggtctgc tatttctaca tcacgattga ttggagctgg
     4801 gtgcatgagg attgctgttt ctttcaaacg atcgtaacgt tcttgagtca agccatgttg
     4861 ggcatggtag tcttcttttg aaaatacagc tccactatca tggcgttcgt gttgcacacg
     4921 gagaaacatc atgacatcaa cctgatcaat gatttcatca atggttacaa actgtccata
     4981 gtctgcaaac tcttgacttc tccattcctc aggtccagcg aaaaagagtt cagctcccaa
     5041 gcgtttcaaa atctgcatat tggatttggc aacgcgtgag tgatccaagt cacctgcaat
     5101 agcaacttta agaccctcaa agtggccaaa ttcctcataa atggtcatca aatcaagcaa
     5161 gctctggcta gggtgttggc ccgaaccatc tccaccattg atgatggaag tcgtaatcgt
     5221 tggactagca atcaattctc tatagtagtc gacctctgga tggcgaatca cacagacatc
     5281 cactcctaaa gcagacagag tcaaaatggt gtcataaagt gtctcaccct tattaaccga
     5341 actagtcttc acatcaaagt caagtcgttc caatccaagt ttaatctctg cgacttcaaa
     5401 ggacttatgt gtccgtgtag aatcctcaaa gaagagattg gaaacaatcg gatggtcttc
     5461 atagggaagc tgggctccat ttttaaactc aattcctcgc ttgatcaatt tcattacttg
     5521 atcgacagtg aggtcttcca tggacaccac atggttcaat gcttgttgat tttctgacat
     5581 ggctactcct ttaactttct aagcttcttc agtaatcaga actctgtctt ggtcatcaag
     5641 ttctgtcatc tctacgatga tttcttcaga acgactggtt gggatatttt ttccaacgta
     5701 atctggacgg attggcaatt ctctatgtcc acgatcgact agaactgcta aactcacacg
     5761 cgcaggacga ccatgaccga caatattatc aatagcagca cggatggtac gacctgtata
     5821 gagcacatca tccaccaaga taacttcgcg gtctgtcaca tcgacagaaa ccaaagaagt
     5881 atcttctcca cttttaacat catcacggaa aggtttagta tccaattcca caacaggaac
     5941 tgaaagattt tctaactgct tcaaacgttc ttggattcgg tgggcaataa agacaccacg
     6001 agttttaata ccagccaaga cgatcttatt caaatctttg ttgcgttcga taatctcata
     6061 agtaatacgc gtaatcgctc gtttgacggt caattcgtct acaacttctt ttgttttcat
     6121 gacaaacctc caaaaagaaa agtctcctta aacaaggaga cttgaaattt atagccaagc
     6181 gagccctact gcacacagta tagacttcac ccttctactt tatcgcgctc cttgcctgcc
     6241 tcacgggaca ggtttaaagg aatatttagt tatcatttac tatagcacaa agcatgctta
     6301 aaatcaagca aaaagtttca atgtagcatc ttacaaattg ctaaaatcat ataattgtgg
     6361 gtactggtca cactctggat tttttggatg gcaaatggct cttccaaaat aaatcatggc
     6421 ctgatgggca gctaaccact gctcaggcgg caagatatcc atgacccgct tttccacctc
     6481 aagtggcgtc gctgattttt tgacaatatc gtggtgtttg caaatacgct ccacatgagt
     6541 atccactgca aaggctggaa tcccaaatcc tacactcatg acaacattgg ctgtcttgcg
     6601 accaacacct gccaaactct ccaattcttc acgtgtctga gggacttgac catcaaaatc
     6661 gtctagtaac tgttgggcac attttttaag gaatttagct ttattccgat acaatcccaa
     6721 gcgagaaata tgtgaagcaa tctcactctc tgtcgctaca gacatagctt ggggtgttgg
     6781 aaaggcaaca aagagacctg gtgtggcctt atttaccgct gcatctgtcg tctgggctga
     6841 taacatgacc gcaaccagga gttcaaaatg attggtaaaa tcaagactag gcttggcatc
     6901 tgggaagagg gtaatgattt cttctagcac ctttcgtgct cgtttttttg acaagaccat
     6961 tattcatctc cgtcaaatag tccttgtaag ccagcaaaag gactgttttc ttctttcttt
     7021 actgcttttt gagcttggta ttcttcctct gtcatgattt gccagtcatt tcctgagata
     7081 aatccttgac cagcttcttc ttcagccgtc aagaccttga taggaatgtt tagcaggata
     7141 ttgtctgata cactctcagc aaggtcaagc tccccatttt cgatgggcaa gaccaagtca
     7201 tcatctaaaa cttcttgatc tagctggtta gttgcgcctt ccatgaaaac ttccgtgact
     7261 ggataagatt caactaactc aactggctcc atactgcgac tcgaagcaag aacaatggta
     7321 taagatagtt gataatctaa gaaatacata cggtcttcat attgtacttt cccaactgca
     7381 aggatatctt ttacatctaa aatttcttga ttacgtgcac acaggtcatc aactaaatct
     7441 aacgtttgtt caaagttcaa accttcagac tgcttacgaa tttcttgaat atttaatttc
     7501 atacttcctc cataaagatt tactctcttg attataccat gaaaaggcta caaatcagca
     7561 caccaaactt tgtaattaaa attcaaaatt ttaacatatt tactattata gttttatttt
     7621 ttagtgctat actataggga aagagtacat cagatcaagg aggatgctca catggaagac
     7681 aagaaactca ttcaactcct atccaagtta aataaaagct accaaaactg taaacagggt
     7741 acggcagatg atattcgact acaagagctg ctaaacacta ctatgcaaga gctcaaaaaa
     7801 acggaacagt tgaacaacag tatcttaatt gatcttgaga aattttacca acctaccagt
     7861 cttctgattg gactgggtag cctaaaacta aacgatcaag cacgcactgc ttggcgaaac
     7921 tatgataaat tccattacga gcatgtcaaa cacgtactaa gtctctatgg acctgttttt
     7981 gaattttaga gcatagaatt tccagttttc tgttgacaaa atttccttaa aggtataata
     8041 taaagatact aatactcgga ggtaagggag acatgaacaa ctaagtctat caaataaaga
     8101 acctttattt agtagatctt gtttttgtct ctttttgtgt gctcttttat gctctttttc
     8161 tggcatgtta atagagtttt tttgacatag actttgggct ctactaggta aagtagagct
     8221 ttttgttatg cactatgaac attctagaaa gggaaatcat atgataaaaa tcaatcatct
     8281 aaccatcaca caaaacaaag atttacgaga tcttgtatct gacctaacca tgaccatcca
     8341 agacggggaa aaggttgcta ttattggtga agaaggaaat ggcaaatcaa ccttacttaa
     8401 aattttaatg ggggaagctt tgtctgattt cactatcaag ggaaacatcc aatctgacta
     8461 tcagtcactg gcctacattc ctcaaaaact tcccgaggag ctgaaaaaga aaactttaca
     8521 cgactacttc tttttagatt ctattgattt agactacagt atcctctatc gtttggcgga
     8581 ggaattgcat tttgatagca atcgtttcgc aagtgaccaa gagattggca atctatcagg
     8641 gggagaagct ttgaaaattc agcttatcca tgagttagcc aaaccctttg agattctatt
     8701 tttagatgaa ccttcaaatg acctagacct tgagacagtt gattggctaa aaggccagat
     8761 tcaaaagacc aggcaaaccg ttattttcat ttcccatgat gaagactttc tttctgaaac
     8821 ggcagacact attgttcact tgcgactggt caaacaccgt aaagaagcgg aaacgctagt
     8881 agagcattta gactatgata gctatagtga gcagagaaag gctaattttg ccaaacaaag
     8941 tcagcaagct gctaacaacc aaagagccta cgataaaacc atggaaaaac atcggagagt
     9001 taagcaaaat gtagaaactg cgcttcgagc taccaaagat agtactgccg gtcgcctatt
     9061 gtctaaaaag atgaaaactg tcctctcaca agaaaaacgc tacgaaaagg cagctcagtc
     9121 catgactcaa aaaccacttg aagaggaaca aatccaactt ttcttttcag acatccaacc
     9181 attaccagct tctaaagtct tagtccaact ggaaaaagaa aatttgtcca ttgacgaccg
     9241 agttttggtt caaaaactac aactaactgt ccgtgggcaa gaaaaaatcg gtattatcgg
     9301 gccaaatggt gttgggaaat caactctgtt agccaaatta aagcaacttc tgaatgataa
     9361 aagagagatt tcccttggtt ttatgccaca agattaccac aaaaaactac aattggattt
     9421 atcaccaata gcttatatca gcaaaactgg ggaaaaagag gaacttcaga aaatccactc
     9481 tcacctagcc agtctcaatt tcagttatcc agaaatgcag catcaaattc gctccttatc
     9541 tggcggacaa cagggaaaac tcctgctttt ggatctagtc ctgcgcaaac caaactttct
     9601 cctgctggat gaacccacac gaaacttttc tcccacttct caaccccaaa tcagaaaact
     9661 ctttgcaacc tatccaggcg gtctcatcac tgtttcgcat gaccgtcgtt tcttaaaaga
     9721 agtctgctcg atcatctatc gcatgacaga acacggtttg aagctagtta atttagaaga
     9781 tttataaatt tgcaacatag caaaaatcca gagacgacct ctggattctt ttacatctgt
     9841 tttaaacgtt caatccgttc tgagataggt gggtgggtat aaaagagttt ttggaacccc
     9901 ccacctttct tgggatcatt gatataaaga gcactgctag catcatcgac gtggcgactc
     9961 atcggtttgc tattgtccaa cttatctagg gcattaatca ttccctgggg attgcgagtc
    10021 agctcaacac tagatgcatc tgccagaaat tccctctgac gagaaatagc gagctgaacc
    10081 aaggttgcag cgagaggtgc cagtacaata gctagtaggg aaaccactag cataatgatt
    10141 tcaagaccat ttccatctcg gtcatcatca cttcgtctgc gacctgctcc accccaccac
    10201 atcatacgac ctgccatacc agaaagcatg gtgatagcac tagcaagggc aactgcaata
    10261 gtcgaaatac ggatatcata attacgaata tgactgactt catgtcccat aacagcttct
    10321 agttcttcac gattcatgat agctagtaga cctgaagtcg cagcaacagc cgcattttga
    10381 ggattagaac ctgtcgcaaa ggcatttaag gctggatcat caatgatgaa aacacggggc
    10441 ataggaatct gagcgaccag agccatatct tccactacat ggtagaggtc tggtgccgtt
    10501 tgctcatcca cctcacgcgc tccattcatg gacatgacaa tctctgtcga ttgaaaaatc
    10561 atagacaaag cgtagataaa gccgataatc agtgcgataa ccaaaccacc aagtccagat
    10621 cttataaaga gataaccaac cgcataacca acaagagcta agagtaggaa aaataccagc
    10681 aacaaaatcc aggtttttcg tttattgctt gcaatttgat caaacaacat cttagtcacc
    10741 taaaccgcta aaatcaactt taggaaccga cttttcctct tcaggtgttt gaaggaaatc
    10801 tgccgcttta aatccaaaca ttccagcgat aatattgctc gggaaagttt ctaattttac
    10861 attgtagttg ctgacaacac tgttatagag ttgacgagag taagaaattt tattttctgt
    10921 gtttgtcaac tcctcttgca atttaacaaa gttagcacta gctttcaaat ctggatagct
    10981 ttctgcaact gcaaaaatac ctgaaacctg acgagtgagg gcatcactgg ctttcatagc
    11041 ttctgctggt gaagtcgctg ccgccacttg gttacgtagt tctgccacct tttcaagggt
    11101 agaaccttca tatttggcat aaccttttac agtctcaatc aagttcggca agaggtcatt
    11161 tcgacgtttc aactgaacat caatctgact ccaagcctcc ttggtttgca tacgattttt
    11221 aaccaaaccg ttatagctaa caatcacaaa aataacaata agagcgataa ctccaagaat
    11281 aatccaagtc ataatataag tcctttctgc ttttagatta gtaccagtat atcaaatttt
    11341 ctatgattgt ggtaaaataa gatgatacta aagaaggaaa taactatgaa accaaaaaca
    11401 ttttacaact tgcttgccga gcagaatctt ccactttcgg accagcaaaa agaacaattt
    11461 gaacgttatt ttgagctctt ggtcgagtgg aatgagaaga ttaatttgac ggcgattacg
    11521 gacaaggaag aagtttatct caaacatttt tacgattcga ttgcacccat tcttcaaggt
    11581 ttgattccca atgaaactat caaacttctt gatatcgggg ctggggcagg atttcctagt
    11641 ctaccaatga aaattctcta tccggagtta gatgtgacca ttattgattc actcaataag
    11701 cgcatcaact tcctacaact cttggctcaa gaactggatt tgaacggagt tcatttctac
    11761 cacggacgtg ccgaagattt tgcccaagac aagaacttcc gtgctcaata tgattttgta
    11821 acagctcgtg cggttgcccg tatgcaggtc ctatctgaat tgaccattcc ctaccttaag
    11881 gttggtggaa aactattggc actcaaggcc agcaatgcgc ctgaggaatt attagaagct
    11941 aagaatgccc tcaatctcct ttttagtaag gtcgaagaca atctcagcta cgccctaccg
    12001 aatagagatc cgcgctatat cacagtggta gaaaagaaaa aagaaacacc aaataaatat
    12061 ccacgtaagg ctggtatgcc aaataaacgc ccactttaaa ttttttagta aacaaatgtt
    12121 tacaaaatca gcctcgctct tttatttcta ggctcgggaa aaaatgattt acaaaatcat
    12181 ttttttctgc tatactatcc taagcaaagg tttttaatgt catcccgtga ggtgacgaag
    12241 acgcagaaat atttaaaact ctttaaaatc taaattttaa agaagtctta ctctgagggc
    12301 ctattgctgt aaaataatgg gctctttttt gatgcccaaa agtgaggttt atatgaaaca
    12361 agaatcaact gttgatttgt tactagacgt tgaccaacgt ccttcggctg gaaaaggaat
    12421 tctccttagt ttccaacacg ttttcgccat gtttggtgcg accatcttgg taccattgat
    12481 tttgggaatg cctgtatctg ttgccctttt tgcttcaggt gttggaacac tcatctacat
    12541 gcttgctact ggttttaaag ttccagttta tctaggttct tcattcgcct ttatcacagc
    12601 tatgtcactg gctatgaaag aaatgggggg ggatgtatct gctgctcaaa caggggttat
    12661 cttgactggt ttggtctatg tccttgttgc taccagcatc cgatttgtag gaacaaaatg
    12721 gattgataaa ctcttgccac caatcattat cggtcctatg atcatcgtta tcggtcttgg
    12781 acttgcaggt tcagctgtta ccaatgcagg tcttgtagca gacggaaatt ggaaaaatgc
    12841 tctggtagcc gttgttactt tcctaattgc tgcctttatc aatacaaaag gaaaaggctt
    12901 cctacgaatc attccattcc tctttgccat tatcggtggt tacctcttcg cactaactct
    12961 tggtttggtt gactttacgc ctgtcctaga agctaactgg tttgaaattc ctggtttcta
    13021 cttgccattt agcacaggtg gtgcctttaa agagtacaat ctttactttg gtccagaagc
    13081 catcgctatc ttgccaatcg ctatcgtaac aatttctgaa catatcggag accatactgt
    13141 tttgggtcaa atctgtggtc gtcaattctt aaaagaacca ggtcttcacc gtactcttct
    13201 tggtgacggt atcgcaactt ctgtttctgc cttccttggt ggaccagcca atacaactta
    13261 cggagaaaat acaggggtta tcggtatgac tcgtatcgct tctgtctcag ttatccgtaa
    13321 cgctgccttc atcgcgattg ccctcagctt ccttggtaaa ttcactgcct tgatttcaac
    13381 tattccaaac gctgtacttg gtggtatgtc aatccttctc tatggggtta tcgccagcaa
    13441 tggtttgaaa gtcttgatta aagaacgtgt tgatttcgct caaatgcgaa acctcatcat
    13501 cgcaagtgct atgttggttc ttggacttgg aggagctatc cttaaacttg gtccagttac
    13561 actttcaggt actgcccttt cagccatgac aggaatcatc ttgaacttga tcttgccata
    13621 cgaaaataaa gactaagagt ctaaatacac ctaatccact cagacagctg agtggatttt
    13681 tcgtatacca taataaaagt gtcttaacaa aattattaaa atcaaaaaac gtataatatc
    13741 agatattcta aaaccttgat actgtacgtt ttatcataga aatttttact ttattttctc
    13801 atcaaatgag atttgcatca atctcttgtc ttacttgcgt ttcttcttcg ctttcttcat
    13861 tttgttagcc atacgtttca tggactgttt catggcaaat tcaccaattt tacctttcaa
    13921 accgccacca aacatctggc tcatatctgg cattcctgct cctccgagag ctgataagtc
    13981 aggcataccg ccttgtccca tcattccttc aagggcagac atatccattc ctcccatatt
    14041 tggcatattt ttaggaaggt tatttggatt aatccccatt tgcttcatca ttttattcat
    14101 atccccagac ataacaccct gcatgagctg tttagcctgg ttaaagtcct tgatgaattt
    14161 attgacttcg acgaatgtat ttccagaacc agcagcaata cgacggcgac ggcttggatt
    14221 taacaaatct gggttttcac gctcttcagg tgtcatcgaa gacacaatgg cacgtttacg
    14281 agcaatctgg cgttcatcca ccttcatgtt ttgaagggct ggattgttgg ccatacctgg
    14341 aatcatcttg agcaagtctt ccatcggccc catattttgc acctgatcta attgatcgat
    14401 gaaatcatta aaatcaaagg tgttttcgcg catcttctca gccatttcaa gggctttttg
    14461 ttcatcgtat tcctgagaag ctttctcaat caaagtgagc atatccccca tgccaaggat
    14521 acggctagac atacggtctg ggtggaaggt ttcgatatct gtaatttttt cacctgtacc
    14581 agtgaacttg attggttttc cagtgatgtg acgaacagac agagcagcac caccacgagt
    14641 atcaccatca atcttggtaa ggatgacccc agtcacttcc aactgagcat taaactcacg
    14701 cgcaacattg gctgcttcct gaccaatcat agcatcaacg acaagcaaga tttcatttgg
    14761 ttgagccaat actttcacat cacgaagctc attcatgagg agctcatcaa tctgcaaacg
    14821 acccgcagta tcaatcaaga catagtcgtt atgattagtt tgggcttgct ccaaaccttg
    14881 acgtacaatc tcaacagctg gtacttctgt tccaagtgca aagacaggca catcaatctg
    14941 ttgtcccaag gtcttaagct ggtcaatggc agctggacga taaatatccg ccgcaatcat
    15001 caaaggacga gcattttctt ctttcttgag tttgttggcc aatttaccag caaaggttgt
    15061 tttaccagcc ccttgtaaac caaccatcat gatgatggtt ggaatcttag gtgacttgat
    15121 aatttctgcc gtatcagaac ctaaaacggc tgtcagttcc tcatcaacga ttttaataat
    15181 ctgttgcgca ggattaagcg tatcaatgac ctcatgcccg actgcacgct cacgaacttt
    15241 cttgataaag tcctttacaa caggcaaggc aacgtcggcc tcgagcaagg ccaagcgaat
    15301 ttctttggtt gcctcttgga catcagattc agagattttt ccttttttac gtagattttt
    15361 aaagacgttc tgcaaacgtt ctgttaaact ttcaaatgcc atttttcttc ctcttattct
    15421 ctattatcaa tgcttgttaa aatttctatc tgctcctgca gaaagtcatc cttgggatag
    15481 cgctccaaaa tctgatcaaa aatctgactg cggacaatat agtccgagta catgtgcaat
    15541 ttcatctcat aatcttccag aatcttttct gttcgcttga tattgtcata gacagcctga
    15601 cgactgacac cgaactcctc ggcaatttca gcaaggctgt aatcatcagc gtagtagagc
    15661 tcgatataat tcatttgctt atctgtcaaa agcgccgcat aaaattcaaa gagcgcattc
    15721 atacgattgg ttttttcgat ttccataact tttattatac caaaaattag cctaatctac
    15781 cacactagga agccgatcca agaagataga tagctaaatt tgaaaaagac atgagcctag
    15841 ccccaagtaa tttccaattg atagctggca aagggatgtc cctcttgatt ttgtagttga
    15901 taatctagtt caatcttttg cccatccact tgataacgat tcgtttggat aataaactcc
    15961 tgcataccca taggtgtggg aatataggct aaactatcac tatcctttag aaagcgcata
    16021 atggtcttgg gattggaaaa tcgactcatc acaagttctt gaccatgaaa tttaatcact
    16081 actttttcct tttcctcatt atagaaaagc aggtagctat aatctccttt ttcatgcact
    16141 tccacatcat aaagctggtc aatcacttcc aactgctcat caaactgaat tgtatttcgc
    16201 atccgaatct tcacatcagg ccctctttct tgtctcttgt cctactattt taccaaaaag
    16261 agcaggattt tgctataatg gtcatatgaa cgaaaaagta ttccgtgacc ctgttcacaa
    16321 ctacatccat gtcaataatc aaatcatcta tgacttgatt aatacaaaag aatttcaacg
    16381 tttgcgccgg atcaaacaac tgggaacttc cagttatacc ttccacggtg gagagcatag
    16441 tcgcttctct cactgtctag gagtctatga aattgcacga cgcatcacag agattttcga
    16501 agaaaaatat cctgaggaat ggaatcctgc cgagtctctc ttgaccatga ccgctgctct
    16561 cctacacgac cttgggcatg gtgcctactc ccatactttt gaaaatctct ttgatacaga
    16621 ccatgaagcc attactcagg agattattca aaatcctgag acagagattc accaagtcct
    16681 gctacaagtg gcacctgatt tcccagaaaa ggtggccagt gtcattgacc atacctatcc
    16741 taataagcag gtcgtgcagc tcatttctag ccagattgat gcggaccgca tggactatct
    16801 cttgcgtgac tcctatttta caggagcatc ctatggggaa tttgacctga ctcgaatcct
    16861 ccgagtcatt cgtcctatcg aaaatggtat cgcctttcag cgcaatggca tgcacgccat
    16921 cgaagactac gtcctcagtc gctaccagat gtacatgcag gtttatttcc accccgcaac
    16981 acgcgccatg gaagttctcc tacagaatct tctcaaacgc gccaaggaac tctatcctga
    17041 ggacaaggat ttctttgccc gaacttctcc acacctcctg cctttcttcg aaaaaaatgt
    17101 gaccttgact gactatctgg ctctggatga tggcgtgatg aatacctact tccagctttg
    17161 gatgaccagt cctgacaaga ttcttgcaga tttatcgcat cgctttgtca accgcaaggt
    17221 ctttaaatcc attacctttt cacaagagga ccaagatcaa cttactagca tgagaaaatt
    17281 ggttgaggat atcggctttg atcccgacta ctacactgcc attcataaga actttgacct
    17341 cccttatgat atctatcgtc ccgaatctga aaacccacgg acacagattg agattttaca
    17401 aaaaaatgga gaactggccg aactctctag cctgtctcct atcgtccaat cccttgctgg
    17461 cagtcgccac ggagataatc gcttttattt tccaaaagaa atgttggacc aaaacagcat
    17521 ctttgcaagc attacccagc aatttttaca cttgattgag aacgatcatt ttaccccaaa
    17581 taaaaactag aagaggaatt ttatgagtat taaactaatt gccgtcgata ttgacggaac
    17641 cctagtcaac agccaaaagg aaatcactcc tgaagtcttt tctgccatcc aagatgccaa
    17701 agaagctggt gtcaaagtcg tgattgcaac tggccgcccc atcgcaggtg ttgccaaact
    17761 tctggacgac ttgcagttga gagaccaagg tgactatgtg gtgaccttca atggtgccct
    17821 tgtccaagag actgatactg gccatgagat tatcagcgaa tccttgacct atgaggatta
    17881 tctggatatg gaatttctca gtcgcaagct tggtgttcac atgcatgcta ttactaagga
    17941 cggtatctat accgccaatc gcaatatcgg aacatacact gtgcacgaat caaccctcgt
    18001 cagcatgcct atcttctacc gcactcctga agaaatggct ggcaaggaaa ttgtcaagtg
    18061 tatgttcatc gatgaaccag aaatcctcga tgctgcgatt gaaaaaatac cagccgaatt
    18121 ttacgagcac tactctatca acaaatctgc tcctttctac ctcgaactcc ttaaaaagaa
    18181 tgtagacaag ggttcagcta ttactcactt agctgaaaaa ctaggattaa ccaaagatga
    18241 aaccatggct atcggagacg aagaaaatga ccgtgccatg ctggaagtcg ttggcaaccc
    18301 tgtagtcatg gaaaacggaa atccagaaat caaaaaaatc gccaaataca tcaccaaaac
    18361 aaatgacgaa tccggcgttg cccatgctat tcgtacattg gtactgtaaa aggaaagaat
    18421 agataaaaac cgctcagctg agcggttttt gatgtctaat tactgccccc tgcaggaatc
    18481 gaacctgcaa ctactcctta ggagggagtt gttatatcca ttgaactaag ggagctagat
    18541 aaaaactctg ctgaaaagca gagtttttta gtcgaattaa cgacggattt ctttgatacg
    18601 agctgcttta ccttgaagag cacgcaagta gtacaatttc gcacgacgta ctttaccgta
    18661 acgaacaact tcgatttttt caacacgtgg agtgtggatt gggaagatac gctcaacacc
    18721 tacaccgtta gagattttac gaactgtgta gttttctgag attccagcac ctttacgtgc
    18781 gataacaaca ccttcaaaaa tctggatacg ttcacggtta ccttcgacaa ctttcgcgtg
    18841 tacacgaaca gtgtcaccag gacggaatga tgggatatct gtacgaagtt gaccttcagt
    18901 caagctttgg attaatggat tcattttatt ctcctatctt tgtcaatctt gaggaatctt
    18961 cctcagcgga taaactgtat ttttgtgcgt ccattacaca caagatatag tttaccaaat
    19021 ttctaaaaaa aagtaaagaa atttatagca aaattcctaa aaaaatcgca accaaaccac
    19081 ctatgtaagt caaagtcagg taagaataaa agaccttctt atcacttagc agtctttgaa
    19141 gttcgtcatt caaggtcgaa aaagttgtta aaccgccaca aaatcctgtt gctagaatag
    19201 catatacttc cttagactct acatgattgt agaatactcc aattaaaaaa caacctaaaa
    19261 gattagctat gagcgttccg agtggcaatt tagaagcttg attatagcga gaaaagaaat
    19321 accgtacgag tgctccgaaa ccacaagcaa ttgcaagata gacgattacc atttcttcct
    19381 ccctagaaaa taagctaaga gtaggcctcc accgatgctc aaaagcaaat acaggactaa
    19441 actaagataa cgccctgtat caagcagctt cacagtatca agcattaggc tggaaaaagt
    19501 tgttaaacct ccgcaaaaac ccgtccccag tgctaaaatc aagcccttac tagtcccctt
    19561 atagaccaga tagcccttga caagaaaaat caagcagaaa attcccagat agttgacaaa
    19621 gagagtgccc caaggaaagt ctggactggc tggtaaccag gtggaaacta gatatcggac
    19681 aagtccgccc aacatagcag ctagaaaaat tcctagcgga taaaattgtt cttttttcat
    19741 ttgatatttt gatcctgata atcacgcgaa cgtttgagta tgtccgaaaa agtcgcgaca
    19801 atagtctcct gatagcgctt gtcctctaca cgactcctaa ccttttcaaa aataacttct
    19861 tctctcttag tatctaaaat cggtttacct gaagctttct tataagcaac caccccctca
    19921 actaaatgca ttcgttcttc taacagtttg acaatttggt cgtcgatttg atcgatttct
    19981 tgacgaataa tatctaaatc catacagtct cctcctttat ttgaattatt gtatcaaaaa
    20041 gccatcaatt aggctagtaa aatcccaact ctcgataaga aaaatgcact gattgattac
    20101 atcagcgcac ttttatgttt atcctacttt agcagccaat tcttctgcga attgttccaa
    20161 gcgttcaata tcttcttcct cggcagaaag atcaacttta acacactctg aacctttttc
    20221 tgctcctgtt gacacaaaga cacggtcaaa gtcatcaaca gccttacaga attcatcgta
    20281 gaaggtatct cctgagccga ccactccgta gattttgcca ttcaagttga gatctgctag
    20341 gtcttcgtag aagtccatca tctcatctgg caattcacca tcaccataag tataggtcgc
    20401 aacgatagcg atgtctgctt ccaagaagtc tgaagcgtca acagttgtac attcatcaac
    20461 atcaacatcc aagcccaagt cacgtaattt gtctgctaca atatctgcaa tttcttcggt
    20521 attaccggtc atactggcaa atacaatttt tgctaatgcc atatcgtcct cctcaattta
    20581 tctttctcca ttatatcaca tctttttcat tttaaaaata aaaattcttg tgctacaatg
    20641 aaatgagaaa gaattgaggt tatttatgga gatttgccaa caaattttag aaaaaattaa
    20701 agaatacgac accattatca ttcatcgtca tatgaaacca gaccctgatg ccttgggaag
    20761 tcaggtggga ttgaaagcct tgctggaaca tcatttccca gaaaaaacca tcaaagccgt
    20821 cggttttgat gaaccaactc ttacttggat ggctgagatg gatcttgttg aagatagagc
    20881 ctaccaaggc gcacttgtca tcgtctgtga tacagctaat actgctcgta tcgatgataa
    20941 gcgctatagt caaggtgatt ttctcattaa gattgaccac catccaaatg atgatgtata
    21001 cggtgacctg tcttgggtcg atactagttc aagtagcgct agcgagatga ttaccctatt
    21061 tgcccaaaca acccaactag ccttggcaga tcgcgatgct gagttgctct ttgcaggaat
    21121 tgtcggtgat acaggtcgct tcctctaccc ttctaccact gcacggactc ttcgcctggc
    21181 tgcttatttg agagaacata actttgactt tgcggctctc actcgcaaaa tggacactat
    21241 gagctacaaa attgctaaac tgcaaggcta catctacgac catctggaag tggatgaaaa
    21301 tggtgctgct cgcgttatcc tgagtcagaa aatcttgaaa caatacaata taaccgatgc
    21361 tgaaactgcg gccattgtag gtgcacctgg acgcattgac agagtgagtc tctggggaat
    21421 ttttgtcgaa caggctgatg gccactaccg agttcgctta cgcagtaaag tccatcctat
    21481 caatgaaatt gccaaggagc atgatggtgg aggccaccct ctagcaagtg gtgctaattc
    21541 ctatagccta gaagaaaacg aaatcatcta ccaaaagtta aaaaacttgc ttaaaaactg
    21601 ataaaatact tgccaaactt ttcagaatct gatagactag tatagtaaca atctatggct
    21661 cgcaaagaga ccatggcaga aaggaaatat tgcaaaatga aaaaagatat ccatccagaa
    21721 tatcgcccag ttgtcttcat ggacacaact actggttacc aattccttag cggttcaaca
    21781 aaacgctcta acgaaacagt tgagttcgaa ggcgaaactt acccattgat ccgtgtggaa
    21841 atttcatcag actcacaccc attctacact ggacgtcaaa agttcactca agcagatgga
    21901 cgcgtggatc gtttcaacaa aaaatacggt ctcaaataat gataagagaa cagttttggc
    21961 tgttcttttt tgtttcttga aatcaactgc tgttttcatg ttccagactc atctgtaggt
    22021 tcgatttcca tgctactagg caggaaggaa atagctgttt caacacgtcc ataatgagct
    22081 atactattgt cacgaaccac actttcattg atggtccaag tggaattcat tttcttaaaa
    22141 gcttctcgga ctttttccaa atctttggag gcaatggcct gctctaaggt ttcaaaacga
    22201 ggacttagac ggtttaccaa tttttccttt tccgcatcta ggtcaacagg attttgttct
    22261 ttttcaaaag ctaatagagc tgaggaaatt tcggtgagtt tggcttcagt aatatctcca
    22321 gatagagcta acttttcctt aacaatctta ccaacctcag aatccttatt ttctactctt
    22381 tcgaagtctg atgccatctt cgtaacgagt ttctgggtct cagtctgacc accattttta
    22441 accgttttgg atgcatctgt gattttcaca aagaaaaaac tcaagctttc ttgggcacct
    22501 gctggggaaa gaattaaaaa tgacaaagct aaaactagta accagctttt agccttccaa
    22561 taatttctga ccaatgtagc ctcctttctc cacaccacca aagcaagtaa aaagtccact
    22621 acctatgtga gtgatgtatt cattcatctt atcagtagca cctaggttag tttgaacctt
    22681 aacaaagtta tcaggatcct tctggaaaga aatgaagagc aatcccgtat caaactgacc
    22741 agtcttttca tcaatgccat ctgagtaaga ataagaacga cgtaaaagtg gcttatccac
    22801 ttctttggcc aaacaaacat gcgaatcatc aggtaagaga ctcaaatcca cttcatcaaa
    22861 ctcatttttc ttaccaaagg gggcaccgct ttccttgtaa cgaccaaagg tattttctcg
    22921 ttcttcgaga ctagtgcggt cccaggtctc taaaaacatc tgaatccgac gaactgccat
    22981 ataagaacca ttttccatcc agtccttact atcagcccag ataacgcggt caaagtcttg
    23041 ctccttggtt ggatttgctg ttccatcctt aaaaccaaag aggttccacg gagtctccat
    23101 tcgatccccg atagctgcaa atccagactg actccaacga aggggcaccg catttctccc
    23161 cttacggatg agattgcgaa tagcgtgaaa ggcaatttgc tcatcatctg cacaagcatg
    23221 aatcacaata tctccaccag tatatttttc acgtaattgc tccttaggaa atagaggtaa
    23281 atccctgaac agtcgagggc gcttgttttc caagttcatc cttttcaaga aacttgcaga
    23341 tacaccaaaa gtcagcgtca aacgatgagg attgagccca actgtctcac cagtatcgct
    23401 cggaggaaag agagcattct gtccatcttt tttaaccaat tccccctcga ccaacttggc
    23461 actataatcc gtccaatcct tgaatagctg gataatttta tctctatccg tcgtatgcag
    23521 gtccaagaca acaaaataga tattcttttg catgggcgta ctatttggta catcaagcga
    23581 accttatgaa aaggtctact ggttttaaag cgctttcttt tcttcttgaa ttgagatttt
    23641 atttttagac gcgacaatgg tttgagcgtc tgctttccta ggatgttctg gacgtggtgt
    23701 aatatttcgc caaccatggc gcttcaacag ttgatagaag gcatcacgtg tgtaggaacg
    23761 acctaactcc tttttataag cctgaaataa ggcatcaatt gtaacaaatt ctcctgcctc
    23821 tgtagccttc aaatgacggg caagaaaggc tttctcttcc tcaactgtca tatatgcatg
    23881 gttacgacca ccacgtgttt cttgaaggag agagtcgagt ccgaactcct catatttttt
    23941 tacatttcgc taaatcgttg tttgattaca
//
LOCUS       DAVLRT010000023        23272 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLRT010000023 DAVLRT010000000
VERSION     DAVLRT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.023"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(35..271)
                     /locus_tag="VXM85_000926"
     CDS             complement(35..271)
                     /locus_tag="VXM85_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1345533.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(339..1259)
                     /locus_tag="VXM85_000927"
     CDS             complement(339..1259)
                     /locus_tag="VXM85_000927"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1345534.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDSDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            1449..1852
                     /locus_tag="VXM85_000928"
                     /pseudo
     CDS             1449..1852
                     /locus_tag="VXM85_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2068..2649
                     /locus_tag="VXM85_000929"
     CDS             2068..2649
                     /locus_tag="VXM85_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1345535.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            2646..3923
                     /locus_tag="VXM85_000930"
     CDS             2646..3923
                     /locus_tag="VXM85_000930"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1345536.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            3941..4603
                     /gene="deoC"
                     /locus_tag="VXM85_000931"
     CDS             3941..4603
                     /gene="deoC"
                     /locus_tag="VXM85_000931"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1345537.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            4590..4979
                     /locus_tag="VXM85_000932"
     CDS             4590..4979
                     /locus_tag="VXM85_000932"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1345538.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            5069..6121
                     /locus_tag="VXM85_000933"
     CDS             5069..6121
                     /locus_tag="VXM85_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1345539.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            6265..7800
                     /locus_tag="VXM85_000934"
     CDS             6265..7800
                     /locus_tag="VXM85_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345540.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINMHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            7793..8851
                     /locus_tag="VXM85_000935"
     CDS             7793..8851
                     /locus_tag="VXM85_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345541.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLTELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            8854..9810
                     /locus_tag="VXM85_000936"
     CDS             8854..9810
                     /locus_tag="VXM85_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345542.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            9895..11243
                     /locus_tag="VXM85_000937"
                     /pseudo
     CDS             9895..11243
                     /locus_tag="VXM85_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(11288..11929)
                     /gene="plsY"
                     /locus_tag="VXM85_000938"
     CDS             complement(11288..11929)
                     /gene="plsY"
                     /locus_tag="VXM85_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1345543.1"
                     /translation="MITIILLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            12065..14008
                     /gene="parE"
                     /locus_tag="VXM85_000939"
     CDS             12065..14008
                     /gene="parE"
                     /locus_tag="VXM85_000939"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1345544.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KVGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRSDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            14428..16899
                     /gene="parC"
                     /locus_tag="VXM85_000940"
     CDS             14428..16899
                     /gene="parC"
                     /locus_tag="VXM85_000940"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1345545.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            17029..18051
                     /locus_tag="VXM85_000941"
     CDS             17029..18051
                     /locus_tag="VXM85_000941"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1345546.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGSVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            18107..18781
                     /locus_tag="VXM85_000942"
     CDS             18107..18781
                     /locus_tag="VXM85_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1345547.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGFAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQIPK
                     RTRKIH"
     gene            18850..19539
                     /locus_tag="VXM85_000943"
     CDS             18850..19539
                     /locus_tag="VXM85_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1345548.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            19536..20459
                     /locus_tag="VXM85_000944"
     CDS             19536..20459
                     /locus_tag="VXM85_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1345549.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            20474..21118
                     /gene="pcp"
                     /locus_tag="VXM85_000945"
     CDS             20474..21118
                     /gene="pcp"
                     /locus_tag="VXM85_000945"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1345550.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            21286..21516
                     /locus_tag="VXM85_000946"
     CDS             21286..21516
                     /locus_tag="VXM85_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1345551.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            21566..21646
                     /locus_tag="VXM85_000947"
     tRNA            21566..21646
                     /locus_tag="VXM85_000947"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21600..21602,aa:Tyr,seq:gta)
     gene            21656..21727
                     /locus_tag="VXM85_000948"
     tRNA            21656..21727
                     /locus_tag="VXM85_000948"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21688..21690,aa:Gln,seq:ttg)
     gene            21889..23091
                     /gene="rpsA"
                     /locus_tag="VXM85_000949"
     CDS             21889..23091
                     /gene="rpsA"
                     /locus_tag="VXM85_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1345552.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
BASE COUNT         7026 a         4092 c         5299 g         6855 t
ORIGIN      
        1 ttcttcaacc cactacagtt gacaaagagc cttcttattt agcaagttta gctgaaagac
       61 gagctttatc gcggcttgct ttgtttttgt gaatcaaacc tttagtttct gctttatcga
      121 tagctgagct agcagcacgg aaaagttctt cagatgggtt tgcttcgaaa gcttttatag
      181 cagtacgcat agctgatttt tgagctgagt tcttttcgtt ttgtttaacg ttcaattcag
      241 cgcgtttgat agctgattta atgtttgcca atgttcttac ctccatattt actaactata
      301 ccattatata tgaaaactta cgttttgaca agggaaaatt atttttttaa gtaaatttca
      361 tcgatttcat ggttcttgct tttatgaaga atcacttctg cacgatttct ggttggttca
      421 atataatttt gtagatttgt gagattgata ctggtccaga cctgatgggc aaaggcttcc
      481 acttccccaa tcggcatctg agtaaaacga taatagtagc tatcagagtc gttttgggct
      541 agactcagca ttttcaagaa acggtccaga taccaacttt caatatcatc cactccagca
      601 tctacataga tggaaaagtc aaagaagtca gtgatataga gacgatcgtt ttgtggattt
      661 tgaaagacat tgattccctc aacaattaca aaatcagcag ctttgacact ttgtttctct
      721 tcgggtacga tgtcgtaaac ttcatgagaa tagacaggaa tatctacatc ttgtccattt
      781 ttgatgcggt ccaagaagtt gagaagagct tccatatcat agctttcagg aaatccttta
      841 cgatttaaaa tcccctgctc aatcaaggtt tgattgggat agagaaaacc atcagttgta
      901 accaactcaa ccgtagcatc tgtaaacgta cgggacagta ggatttgaag taggcgactg
      961 gttgtggatt ttccaacggc aacactccca gaaaccccaa taataaaagg ttgagattta
     1021 ctttcacgtt ggaggaaaat tccttttgaa aaggctaaat cttccttagt tcgcttgtaa
     1081 atctgaatca gatgagccaa ggggagatag atatctgtaa cgtcttggag actgatttgg
     1141 tcattaaaac tcttgataga ttccaattct tcttctgtca aaggaggtgt tgtctttcga
     1201 tgtaaagatt gccaagtctg gcggctgatt ttttcaaaat gtaaaaattc gttggtcatt
     1261 tgttttcccc tagatatttt gtctatcata ccataaaaag ctaaaaaaat aaagcggttt
     1321 cttgaatgtg gtaagtcaat ggtatatagt ataagtgatc aatttattga tgaaatagga
     1381 gatagattct actgatttat aagcttataa agggggcttt ttagttcaca acttatagga
     1441 ggtgtactat gaaaatctta aaacgttaca tattggaact ctgttttatt ttaagttttg
     1501 ctttaccttt tataaaagga gccaatgcag ataatggtag atgctttgtg gaaacctatt
     1561 acggttttac ttttttgatg gaacatgcta ttgtaacagc tgtctttatc tgttcgttct
     1621 taattgcttt cttactaaaa aacgatgggc gaaatggatt gctgcgggta gttattgctt
     1681 tttagtccta cggattgcga cagagggtta tttcttccac atgtcactag aagatttgat
     1741 acgactttgg acaagtttgg aaatcctgac acaaacgtat cagttgggct tttatctaaa
     1801 cattttgtta ggaattttgt tgataataaa gtattttaag gttaagcaat agaaataata
     1861 atctgcctga ttatagatga taaacttttt gtttttatct ggactgtgat atttatagtt
     1921 ctaaatggtt tattatttat aagtgttgaa agagattgaa gaaaatggct tcgtgacttg
     1981 ctttcttcgg tctttttcgt tttattatca atttgtaaac gatttacaat ttgagtcaaa
     2041 ttatggtaaa atagtgtcat gagtaaaatg tactatgcag aaaatcctga cgctgctcac
     2101 gacattcatg agttgagagt ggacttgttg ggagaaaaaa tgactttttt gacagatgcg
     2161 ggtgttttta gcaagaaaat ggttgacttt ggaagtcaac tcttgctcaa gtgtctggat
     2221 gtcaaccaag gagagacagt cctagatgta ggctgtggct atggtccatt aggtttgtcc
     2281 ttggtcaagg tttacggagt tcaggcgacc atggttgata ttaacactcg tgccctggat
     2341 ttagctcaga gaaatgctga aaaaaataat gcaaaagcga cgatatttca atctaatatt
     2401 tatgcaagag ttcaagggcg atttgaccat gtcatttcca atcctcctat ccgtgcgggc
     2461 aagcaagtcg ttcatgaaat catcgagaaa agtaaagatt tcttggaaat tggtggagat
     2521 ttgaccatcg tcatccagaa aaaacaagga gctccaagtg ccaagtctaa gatggaagat
     2581 gtgtttggca attgtgaaat ccttaaaaaa gacaagggat actatatcct tagaagtgtg
     2641 aaagaatgag agcagttgat ttaatccaaa aaaaacgaga cggtcaagaa ctgacttcaa
     2701 gtgaaattga atggctgata gaaggctatg tatcaggaac tgttcctgat tatcagatgt
     2761 ctgcctttgc tatggctgtt tattttaaag gaatgacgac tcgagaaatc tctgatttaa
     2821 cgatgaatat ggttaagact gggcaagagt ttgacctgtc agccattgat ggtgtgaaag
     2881 ttgacaagca ttctactggt ggtgtcggcg ataaggtaac cttgatttta gctcctcttg
     2941 ttgctagctt cggtgtgcct gtagcaaaaa tgagtggtcg tggtcttggc catacaggcg
     3001 gaacaattga taaattggag tccattaagg gctatcaagt agaacgtagt caagaggatt
     3061 ttattcgtca ggttcaggat attggagtat ctgtcattgg gcaatcagac cagctggtca
     3121 aagcagataa gcttctctat gcccttcgtg atgtgaccgc aactgtcgac acgattcctt
     3181 tgattgcgag ttcggtgatg agcaagaaaa ttgcggcagg ggcggatgct attttgctag
     3241 acgtgactgt cggtgagggt gccttcatga agacggttga tgaggcgcgt gagttggctc
     3301 aaactatggt agatcttggt aaggttgttg gacggaagac agtagcagtg attacggata
     3361 tgagccagcc tttgggacga gcgattggaa atcgtctgga aatccttgaa gcattggaga
     3421 ttttgcaagg tcaaggacgt caggatatta cccactttat ttgtgaattg gctcaaatta
     3481 tgcttggttt ggcaaatgtt aacaaaactg ttgaagaagt tcgccaacat cttgaaaatg
     3541 gtcaagcact ggctaagttt gaggaaatgg tgcaagccca aggtggagac ttggaagacc
     3601 tctatcgtcc tgtaaatgta gctcatgtgg tggaaatccc tgctcaggaa acgggtgtca
     3661 tttcagctct tccagctatg gactttggtc tttatgccat gaggctgggt gctggtcgtg
     3721 cagttaagtc tgatgtcttg gactatgaaa caggaattgt ttttgaaaag aagattggag
     3781 actccgttca aaagggtgaa attgttgcaa aagtatacac aaatgaaaaa attccacctc
     3841 aactagttac agatttccaa aaatgtgtta aaataagtga tgaagtgaaa aaaatccgtg
     3901 aaattgtaga aatcatctcc taaatggagg gaaaaccgaa atgaaattaa ataaatatat
     3961 agatcatacg cttttaaaac aagatgcaaa gaaaaaacaa attgatagtt tgttgtctga
     4021 ggctagagag tatgactttg ccagtgtttg cgttaatccg acctgggttg aacatgctaa
     4081 aaaaggactt gaaggcacag atgttaaggt ttgcacagta gtaggtttcc ctttgggagc
     4141 aacaacttca gccgtgaaag catttgagac aaaagaagct atccaaaatg gtgcagatga
     4201 gattgatatg gtgatcaatg ttggagctct caaatcaggt aatttagcct tggttgagtc
     4261 agatattcgc gcagtagtgg aagcaagtgg tgataagtta gtgaaagtca ttattgaagc
     4321 ttgccttctg acagaccaag aaaaaattgt tgtttgccaa ttggcccaaa aagctggggc
     4381 tgactttgtt aaaacatcta ctggcttttc aactggtggt gctacgatag cagatgttag
     4441 attaatgcgt gaaacagttg gatctgatat gggagtcaag gctgctggtg gagctcgttc
     4501 ttatgcagat gctcttgcct ttgtcgaagc aggtgcgacc cgtatcggaa cgtcagctgg
     4561 ggtagctatt ttaaaaggag aattggcaga tggcgactac tgagttgatt gaactggcaa
     4621 ttgaaaccag caaacatgcc tatgtcccct attctcactt tcctatcgga gcagttttag
     4681 tagccaaaga cggaagtgtt tacacgggag tgaatatcga aaatgctagc tatcctttga
     4741 ccaattgcgg tgagagaaca gctgttttta aggctatatc tgaaggccaa agagaatttt
     4801 cagaattgat tgtctatggt caaactgaaa aaccgatctc gccatgtggt gcttgtcgcc
     4861 aagtaatggt cgaatttttt gaacaagatt taaaagtgac cttagtcgca aaagataaat
     4921 cgacggtcga gatgacggtc ggggaattac ttccatactc ttttacagac ttaaactagt
     4981 ctgagtcgct ctctgagtgg cacggtcctt gtggccaacc aatccatact tgcaacatcg
     5041 ttgcacatct tatttaggag gttcagtaat gaacaagaaa caatggctag gccttggtct
     5101 agttgcagtg gcagcagttg gacttgctgc atgtggtaac cgctcttctc gtaacgcagc
     5161 ttcatcttct gatgtgaaga caaaagcagc aatcgtcact gatactggtg gtgttgatga
     5221 caaatcattc aaccaatcag cttgggaagg tttgcaggct tggggtaaag aacacaatct
     5281 ttcaaaagat aacggtttca cttacttcca atcaacaagt gaagctgact acgctaacaa
     5341 cttgcaacaa gcggctggaa gttacaacct aatctttggt gttggttttg cccttcataa
     5401 tgcagttgaa gaagcagcta aagaacacac tgacttgaac tatgtcttga ttgatgatgt
     5461 gattaaagac caaaagaatg ttgcgagcgt aactttcgct gataatgagt caggttacct
     5521 tgcaggtgtg gctgcagcaa aaacaactaa gacaaaacaa gttggttttg taggtggtat
     5581 cgaatctgaa gttatctctc gttttgaagc aggattcaag gctggtgttg cgtcagtaga
     5641 cccttctatc aaagtccaag ttgactacgc tggttcattt ggtgatgcag ctaaaggtaa
     5701 aacaattgca gccgcacaat acgcagccgg tgcagatatt gtttaccaag tagctggtgg
     5761 tacaggtgca ggtgtctttg cagaggcaaa atctctcaac gaaagccgtc ctgaaaatga
     5821 aaaagtttgg gttatcggtg ttgaccgtga ccaagaagca gaaggtaaat acacttctaa
     5881 agatggcaaa gaatcaaact ttgttcttgt atctactttg aaacaagttg gtacaactgt
     5941 aaaagatatt tctaacaagg cagaaaaagg agaattccct ggcggtcaag tgatcgttta
     6001 ctcattgaag gataaagggg ttgacttggc agtaacaaac ctttcagaag aaggtaaaaa
     6061 agctgtcgaa gatgcaaaag ctaaaatcct tgatggaagc gtaaaagttc ctgaaaaata
     6121 atggatggaa gtcatttctt aggaagcggc caagggccgc ttttttaaga gttgagacaa
     6181 agtcattttg tctcagtaaa gcttgctact agacaaacta gcaagtttta ttgaaataaa
     6241 ataagactct gaaaggaaga gcacatggca cacgaaaatg tcattgagat gcgtgatatt
     6301 accaaggtgt ttggtggatt tgttgccaac gacaaaatca acatgcacct acgaaaaggt
     6361 gaaattcatg cacttttagg agaaaatggg gctggtaagt ccacgctaat gaacatgtta
     6421 gcaggccttc ttgaaccaac tagtggtgaa atcgcggtca acggtcaagt tgtcaatctc
     6481 gactccccat ctaaagcagc tagcttggga atcgggatgg ttcaccagca ctttatgttg
     6541 gttgaagcct tcacagtggc tgaaaatatc attttaggta gtgaattgac taaaaatggt
     6601 gtgctagata tcgctggagc tagcaaagaa atcaaggctc tttctgaacg ttatggctta
     6661 gctgttgacc cttctgccaa ggtagcagat atctcagttg gagcccaaca acgtgtagaa
     6721 attttaaaaa cactttatcg gggggctgat atccttatct ttgacgaacc aacggctgtt
     6781 ttgactccat cagaaattga tgagttgatg gctattatga aaaatcttgt caaagaggga
     6841 aaatcaatta tcttgattac ccacaaattg gatgaaattc gagcagtttc tgaccgtgtt
     6901 acagttatcc gtcgtgggaa atcaattgaa accgttgaaa ttgcaggggc taccaatgct
     6961 gatttggcgg aaatgatggt aggacgttct gtttccttta aaacagagaa gcaagcctct
     7021 aaaccaaaag aagtggtttt gtctatcaaa gatttggtgg tcaatgaaaa ccgtggtgtt
     7081 ccagctgtta aaaatctgtc cttggatgtt cgtgctggag agattgttgg tattgcgggg
     7141 attgatggaa atggtcagtc tgaactgatt caagccatta caggtcttcg taaggttgaa
     7201 tctggtagca ttgagctaaa aggagattca attgtaggct tgcacccacg tcagattaca
     7261 gaactaagtg ttgggcacgt tccagaagac cgtcaccgtg atggcttgat tttggaaatg
     7321 atgatatctg aaaatattgc ccttcaaacc tactataaag aaccacatag taaaaatgga
     7381 attttgaact attcaaatat tacttcttat gctaaaaagc tgatggaaga gtttgatgtt
     7441 cgcgctgcca gtgaattagt tcctgcagct gcactctcag gaggaaatca acaaaaagca
     7501 attattgctc gtgaaattga tcgagatcct gatctcctta tcgttagtca gccaactcgt
     7561 gggttggatg tcggtgccat tgagtatatc cacaaacgct tgattgaaga gcgtgataat
     7621 ggcaaggctg tccttgttgt cagctttgaa ttggatgaga ttttaaacgt ctcagaccgt
     7681 attgccgtta tccacgatgg taagattcaa ggtattgtat caccagaaac aaccaataaa
     7741 caagaacttg gtgtcttgat ggctggtgga aacttgggaa aggagaagag tgatgtctaa
     7801 aaaattacaa caaatttcgg ttcccttgat ttctgtattc ctaggaattt tactcggagc
     7861 cattgtcatg tggatcttcg gttatgatgc tatttggggc tacgaagaat tgttctatac
     7921 agcctttggc agtctgcgtg ggattggaga aatcttccgt gctatgggtc ctctggtctt
     7981 gattggtctt ggttttgccg ttgccagtcg agctggtttc tttaacgtcg gacttcctgg
     8041 tcaggctttg gcaggttgga ttctcagtgg ttggtttgcc ctgtcgcatc cagatatgcc
     8101 ccgtcccttg atgattctag caaccatcgt gattgccttg attgctggtg ggattgtcgg
     8161 agcgattcca ggtattctta gggcctatct agggacgtca gaggttattg taaccatcat
     8221 gatgaactac attgtcttgt atgtagggaa tgcctttatc catgctttcc ctaaagactt
     8281 catgcaaagt acagattcga ccattcgtgt tggggctaat gcaacctatc agacaccttg
     8341 gttgactgag ttgactggta actcacggat gaatattggt attttctttg ccatcattgc
     8401 cgttgcagtt atttggttca tgctcaagaa aacaactctt ggttttgaaa tccgtgcagt
     8461 tggtcttaat ccacatgctt cagaatatgc tggtatttct gccaagcgga ctattatcct
     8521 atctatgatt atttcaggtg ccttggcagg tcttggtgga gctgttgaag gtttgggaac
     8581 cttccagaac gtctatgttc aaggttcgtc attagctatc ggatttaacg gaatggcggt
     8641 tagtttgctt gcggccaact caccaattgg tatactcttt gcagccttcc tatttggcgt
     8701 tctccaagtt ggggctcctg gtatgaatgc ggcgcaggta ccatctgagc ttgtcagcat
     8761 tgtaacagcg tctattatct tctttgtcag tgttcattac cttatcgaac gctttgtcaa
     8821 accgaaaaaa caagttaaag gaggtaagta aagatgtcta ttataacctt gctccccctc
     8881 ttggtgtctt ctatgctgat ttactcagca cctctcatct ttacaagtat cggtggtgtt
     8941 ttctctgaac gtggtggtgt ggtaaacgtt ggccttgaag gaattatggt tatgggtgcc
     9001 ttttctggag ttgtctttaa ccttgaattt gcagaacaat ttggagcagc aactccatgg
     9061 ctatccttgc ttgtagcagg attggttggt agtgttttct ctatcatcca cgcagcagcg
     9121 acggttcatt tccgtgcaga ccatgttgtc agcggtacgg tattgaactt gatggcgcct
     9181 gccttggctg ttttcttagt taaagttctt tataacaaag gacaaaccga caacctaagt
     9241 caaacttttg gacgctttga tttcccagtc ttggcaaata tcccagtgat tggtgatatc
     9301 ttcttcaagt caactagtct acttggttat ctggcgattg ccttctcatt ccttgcttgg
     9361 tttattctct ttaagactca atttggtctt cgtctccgct ctgtcggtga acatcctcaa
     9421 gcagcggata ccttgggaat caacgtctac aagatgagat atttaggggt tattatttca
     9481 ggttttctag gtggaattgg cggagcgatt tatgcgcaat caatctcagt taacttctca
     9541 gtgacaacta ttgttggacc tggatttatc gcccttgctg cgatgatctt tgggaaatgg
     9601 aatccaatcg gtgctatgct ttctagtctc ttctttggac tttcacaaag tttggctgtt
     9661 atcggttctc aattgccgtt cctacaagga gtgccagcgg tttatcttca aattgcacct
     9721 tatgttttga caattcttgt cttagcagcc ttctttggaa aagcagtcgc accaaaagca
     9781 gatggtatca actacatcaa atcaaaataa gcatacaaaa aaacgtcagt ccaatgactg
     9841 aactacaccc caaaagttag acagaaaaaa tctaactttt ggggtgtttt tattatgaaa
     9901 ttaacttatg atgataaagt tcagatctat gaacttagaa agcaaggata tagcttagag
     9961 aagctttcaa ataaatttgg gataaataat tctaatctta ggtacatgat taaattgatt
    10021 gatcgttacg gaatagagtt cgtcaaaaaa gggaaaaatc gttactattc tcctgattta
    10081 aaacaagaaa tgattaataa agtctgacat gaaggctgga ctaaagatag agtttctctt
    10141 gaatacggcc tcccaagtcg tacgatactt cttaactggc tagcacaata caggaaaaac
    10201 gggtatacta ttgttgagaa aacaagaggg agagtaccta aaatgggacg taagccaaaa
    10261 acgagacctg aagagaggac agaattagaa tatcttcaag cagaaaatga gtacctgaga
    10321 gcggagaatg ccatcctaaa aaagttaaga gaactccgat tgaaggagga aaaagagaaa
    10381 gaagaaggac agaaattgtt caagaattaa tgactgagtt ttcgttagtt cttcttctaa
    10441 aatccattaa actagctcgt tggacctact actatcactt gaaacagcta gacaaaacag
    10501 ataaggacca agagcttaaa gctgaaattc aatccatctt tatcgaacac aaaggaaatt
    10561 atgcttatcg ccgggttcat ttagaactaa gaaatcgtgc ttatctggta aatcataaaa
    10621 gagttcaagg cttgataaaa gtactcaatt tacaagctaa aatgcgacag aaacgaaaat
    10681 attcttctca taaaggagac gttggtaaga aagcagagaa tctcattcaa ggccaatttg
    10741 aaggctctaa aacaatggaa aagtgctaca cagatgtgac agaatttgcc attccagcaa
    10801 gtactcaaaa gctttactta tcaccagttt tagatggctt taatagcgaa attatcgcct
    10861 ataatctttc aacttcaccc aacttagaac aagtacaaac aatgttggaa caggtattca
    10921 cagagaagca ctatgagaat acgattctcc atagtgacca aggctggcaa taccgacacg
    10981 attcttatca tcggttccta gagagtaagg gaattcaagc atctatgtca cgtaagggta
    11041 acagcccaga caatggtatg atggagtcct tctttgggat tctgaaatcg gaaatgtttt
    11101 acggttatga gaagtcgttt cagtcgctta agcaattgga acaagctatt gtagactatg
    11161 ttgattacta caacaataaa cgaattaagg taaaactaaa aggacttagt cctgtgcagt
    11221 acagaactaa atcctttgga taaattattt gtctaacttt tggggggcag ttcacagaac
    11281 tggcatttta ttttttagga tcttgatggg ttaggttcaa tccccaaggg accaaatttt
    11341 cagttttatt tttgatacga gctatattgt ccttatgacg aatgataatc aaactagcaa
    11401 gtgctaagat aatagcgatg aagagagagt catagttact caggataaaa ccaaaaagtg
    11461 gaaagagcag aaccccgata acagccgcga tcgatgctgt gacactagac agtgaaatca
    11521 tactgccaag atagagagct ccaaagaaga taatcgcaag gtagagacag aagataggcg
    11581 caaatccgaa aatcactcca gcactggttg cgacagcctt accaccttta aatcctgcaa
    11641 agatagggaa ggtatggccg ataacagcca aaagtccaaa gatgagagga gaaacgcctt
    11701 gtagatgaaa aataatcgga agcagcgttg ctagggttcc tttgaaaaag tcaatcacaa
    11761 aggttgccat accagctttc ttacctaaaa tgcggaaggt attggtcgtt ccagtgttac
    11821 cagaaccatg ctcgcgtaga ttgatttgaa agaatacttg tccaatccag agaccagatg
    11881 gaatcgaacc cagcagatag gctaggatta ataaaattat tgtaatcata ctcctattat
    11941 atcatgaatt gggaaagaaa agcagagaat ctctgctgaa attgtcacat cggagaaaga
    12001 aaaattttgc aaaatccttg gaaaacctgt agaatagtaa agatgaacga ataggaggtt
    12061 ccttgtgtca aaaaaggaaa tcaatattaa caattataat gatgatgcta ttcaggtgct
    12121 agaagggttg gatgcggtcc gaaaacgtcc agggatgtat attggatcga ccgatggcgc
    12181 tggtcttcat cacctagttt gggaaatcgt tgataatgca gtcgatgaag ccttgtctgg
    12241 gtttggtgat cgtattgatg taactatcaa taaagacggt agtctaacgg ttcaagacca
    12301 tggacgtggg atgccgacag gtatgcacgc tatgggaatt ccaactgttg aggttatctt
    12361 taccattctt catgccggag ggaaattcgg tcaaggtggc tataagacat caggtggact
    12421 tcacggagtg ggttcttccg ttgttaacgc cctttctagc tggttagaag ttgaaattac
    12481 ccgtgatggc gcagtttaca agcaacgttt tgaaaatggt ggaaaacctg tcacgacttt
    12541 gaagaaagtc ggtacagcac ccaagtctaa aacaggcacc aaagttactt ttatgcctga
    12601 cgcgactatc ttttctacga cagatttcaa gtacaatacc atttcagagc gccttaatga
    12661 atcagccttt ctcttgaaaa atgtgacctt gtctttaacg gacaagcgat cagatgaagc
    12721 gattgagttc cactatgaga atggagtaca agattttgtt tcttatctca acgaagataa
    12781 ggaaatcttg acgccagttc tttactttga aggggaagac aatggttttc aagtggaagt
    12841 agccctccag tacaatgacg gattctcaga taacattcta tcctttgtca ataacgttcg
    12901 caccaaggac ggtggaacgc acgagacagg actcaagtct gccattacca aggtcatgaa
    12961 tgactatgca cgtaaaacag gtcttctcaa ggaaaaagat aaaaaccttg aaggttcaga
    13021 ctatcgtgag ggactagcgg ccgttctttc tatcttagtt cctgaagaac acttgcagtt
    13081 tgaaggacag accaaggata aactaggaag ccccttagct cgcccagttg tggatggaat
    13141 agtggctgat aagttgacct ttttccttat ggaaaatggg gaattagctt ctaacctcat
    13201 ccgcaaggct atcaaggccc gtgatgctcg tgaagcagca cgtaaggcgc gtgatgagag
    13261 ccgaaatggg aagaaaaaca agaaagataa gggcttgttg tctgggaaat tgaccccagc
    13321 ccaatctaag aatcctgcta agaatgaact ctatctagtt gagggggact ctgccggtgg
    13381 ttctgccaaa caaggtcgtg accgcaagtt ccaggctatt ctacctcttc gtggtaaggt
    13441 tatcaataca gccaaggcca agatggcgga tatcctcaaa aatgaagaga tcaataccat
    13501 gatttatacc attggtgcgg gtgttggagc agacttctct attgaagatg ccaactatga
    13561 taagatcatt atcatgaccg atgcggatac cgacggtgcc catatccaga ccttgctctt
    13621 gacatttttc taccgttaca tgcgtccgct agtcgaggca ggtcatgtct atattgccct
    13681 cccacctctt tacaagatgt ccaaaggtaa aggcaagaaa gaagaagtgg cctacgcttg
    13741 gacggacgga gaactagaag aactacgtaa acagttcggt aaaggcgcta ccctccaacg
    13801 atacaaagga cttggtgaga tgaatgcgga ccagctctgg gaaacaacca tgaacccaga
    13861 aacacgtacc ctcatccgtg tcacaattga agatttagcg cgcgccgaac gtcgcgtcaa
    13921 tgttctcatg ggagataagg tagaaccacg ccgtaaatgg attgaagata atgtcaagtt
    13981 tacgctagaa gaagcgacag tgttttaatg aaagaaaata atatgttagt aaactggaaa
    14041 ttagagtcgg atgtgaatga ctatgtgaaa aagcagtttg aaaatcttgg tttgaaaaaa
    14101 ttgcagaatt attaatgaag aatcttctat gagtgcttat ctcaaagagg cgtttaaggg
    14161 agcggctaag acacaaacta agactaattt tggaaaacca gattttcata ttgaaaaata
    14221 caaacaggat agtttggtta cttatcgtaa ctgaacacgg gacgaattcc cgtaaaatat
    14281 cttttactgc ataaaaacct actctacatt ctttgaaagg agttgaacac gccctagata
    14341 ttgtgtgaaa aagataaatt ctcttgtgag tttgcttact tcaagaattt tctattttca
    14401 cttggtattt tatgggcttt gtatcttatg tctaacattc aaaacatgtc cctggaggac
    14461 atcatgggag agcgctttgg tcgctactcc aagtacatta ttcaagaccg ggctttgcca
    14521 gatattcgtg atgggttgaa gccggttcag cgccgtattc tttattctat gaataaggat
    14581 agcaatactt ttgacaagag ctaccgtaag tcggccaagt cagtcgggaa catcatgggg
    14641 aatttccacc cacacgggga ttcttctatc tatgatgcca tggttcgtat gtcacagaac
    14701 tggaaaaatc gtgagattct agttgaaatg cacggtaata acggttctat ggacggagat
    14761 cctcctgcgg ctatgcgtta tactgaggca cgtttgtctg aaattgcagg ctaccttctt
    14821 caggatatcg agaaaaagac agttcctttt gcatggaact ttgacgatac ggagaaagaa
    14881 ccaacggtct tgccagcagc ctttccaaac ctcttggtca atggttcgac tgggatttcg
    14941 gctggttatg ccacagacat tcctccccat aatttagctg aggtcataga tgctgcagtt
    15001 tacatgattg accacccaac tgcaaagatt gataaactca tggaattctt gcctggacca
    15061 gacttcccta caggggctat tattcagggt cgtgatgaaa tcaagaaagc ttatgagact
    15121 gggaaagggc gcgtggttgt tcgttccaag actgaaattg aaaagctaaa aggtggtaag
    15181 gaacaaatcg ttattactga gattccttat gaaatcaata aggccaatct agtcaagaaa
    15241 atcgatgatg ttcgtgttaa taacaaggta gctgggattg ctgaggttcg tgatgagtct
    15301 gaccgtgatg gtcttcgtat cgctatcgaa cttaagaaag acgctaatac tgagcttgtt
    15361 ctcaactact tatttaagta caccgaccta caaatcaact acaactttaa tatggtggcg
    15421 attgacaatt tcacacctcg tcaggttggg attgttccaa tcctgtctag ctacatcgct
    15481 caccgtcgag aagtgatttt ggcgcgttca cgctttgaca aagaaaaggc tgagaaacgt
    15541 ctccatatcg tcgaaggttt gattcgtgtg atttcgattt tggatgaagt cattgctctt
    15601 atccgtgctt ctgagaataa ggcggacgcc aaggaaaacc tcaaagttag ctatgatttt
    15661 acggaagaac aggctgaggc tatcgtaact ttgcaactgt accgtttgac caataccgat
    15721 gtggttgtct tgcaggaaga agaagcagag cttcgtgaga agattgctat gctggcggct
    15781 attattggtg atgaaaggac tatgtacaat ctcatgaaga aagaacttcg tgaggtcaag
    15841 aagaaatttg caactcctcg tttgagttct ttagaagaca ctgcgaaagc aattgagatt
    15901 gatacagcta gtcttatcgc tgaggaagat acctacgtca gcgtgaccaa ggcaggttac
    15961 atcaagcgta ccagtccacg ttcctttgcg gcttccacct tggaagaaat tggcaagcgt
    16021 gatgatgacc gtttgatttt tgttcaatct gccaagacaa cccagcacct cttgatgttc
    16081 acaagtcttg gaaatgtcat ctacagacca atccatgagt tggcagatat tcgttggaag
    16141 gacatcggag agcatctgag ccaaaccatc acaaactttg aaacgaatga agaaatcctt
    16201 tatgtggaag tactggatca gtttgacgat gcgacaacct actttgcagc gactcgcctt
    16261 ggtcaaatca aacgggtaga gcgaaaagaa ttcactccat ggcggaccta tagatctaag
    16321 tctgtcaagt atgctaagct caaagacgat acagatcaga ttgtagcagt ggctccgatt
    16381 aaactagatg atgttgtctt ggttagtcaa aatggttatg ccctgcgttt caatatcgaa
    16441 gaggttccgg ttgtcggtgc taaggcagca ggtgtcaagg ctatgaattt gaaagaagat
    16501 gatgtcctcc aatctggctt tatctgtaat acttcgtcct tctacctctt gacccagcgt
    16561 ggaagcttga aacgtgtttc cattgaggaa attctagcaa ccagccgtgc caaacgagga
    16621 ttacaagtct tgcgtgagtt gaaaaacaaa ccgcatcgtg tcttcttggc aggagcagtt
    16681 gcagagcaag gatttgttgg cgatttcttc agtacggaag tggatgtgaa cgaccaaact
    16741 ctgcttgtcc aatccaataa aggaacaatc tatgaaagcc gattgcaaga cttgaacttg
    16801 tcagaacgca ctagcaatgg aagcttcatt tctgacacga tttcagatga agaagttttt
    16861 gacgcttatc ttcaggaagt agttactgaa gataaataag tgaaatgaag aatcagttct
    16921 aagatggttt ttattggagg aattttctga aaattacaaa atatacttgc tattttagaa
    16981 aaatagtgta gaatataaga aataggtttt tagaataagg agtggaatat gacagtaacg
    17041 attgattggg aaaacctcgg tttttcctat atgaaattac cttatcgcta tcttgctcat
    17101 ttcaaaaatg gacaatggga tcaaggagag cttacagagg atgcaacttt gcatatttca
    17161 gagtcttctc caagtcttca ctatggacaa caagcatttg aaggtttgaa agcttatcgt
    17221 actaaggatg gcagtgttca actgttccgt cctgatgaaa atgctaaacg cctgcaacgt
    17281 acatgtgacc gtctcttgat gccacaagtt ccgacagaca tgtttgtaga agcttgtaaa
    17341 gcagttgtcc gtgcgaatga agaatacgta ccaccatacg gaataggtgg aaccttatat
    17401 cttcgccctc ttttgattgg tgtcggagat attatcgggg taaaaccggc agaagagtac
    17461 attttcacca tctttgctat gccagttgga aattacttta aaggtggttt ggtcccaacc
    17521 aacttcttga ttcaggatga gtacgaccgt gcagcaccaa atggtacagg tgcggctaag
    17581 gttggtggaa actatgctgc aagtctctta ccaggaaaaa tggccaagtc acgccatttc
    17641 tcagatgtta tctatctgga cccatcaact catacaaaga ttgaagaagt cggatcagct
    17701 aatttctttg gaattacagc tgataatgaa tttgtaacac cattgagtcc atctatcttg
    17761 ccatctatta ccaagtattc cttgctttat ttggcagaac accgcttggg attaactcct
    17821 attgagggtg atgttccaat tgataatctt gaccgttttg tagaggcagg tgcctgtggt
    17881 acagcagcgg ttatttctcc aattggaggt attcaacatg gtgatgattt ccatgtattc
    17941 tatagtgaaa cagaagtagg ttctgtgacg cgtaaattat ataatgaatt gacgggtatt
    18001 cagtttggcg atattgaagc gccagaaggt tggattgtaa aagtagatta aaataaaccg
    18061 aaggagattt tttatgaaat agaaaaagtg gctcttaaca gcaggagtgg tcctgagcac
    18121 gtcagctatt ttagtggctt gtggaaaaac tgataaagaa gccgatgcac cgacaacatt
    18181 ttcttatgtc tatgcagtag atccagcatc attgggctac agtatagcga ctcgaacatc
    18241 gacgacagac gttattggaa atgttattga tggtttgatg gaaaatgata aatacggcaa
    18301 tgttgctcct tctcaaaaag actatgattt gaatagtaca ggatgggctc caagctatca
    18361 agatccagcg tcttacttga atattatgga tccaaaatct ggttttgcca tgaaacacct
    18421 tggtattacg aaaggaaaag ataaggatgt tgtagctaaa cctggtttgg ataaatataa
    18481 gaaattgtta gaagatgctg tttctgagac cactgaccta gagaagagat atgaaaaata
    18541 tgccaaagct caagcttggt cgacagatag ttcattattg atgccaacag cttcatctgg
    18601 tggttctcca gttgtaagta acgtagtacc attctcaaaa ccatactcac aagttggtat
    18661 taagggggat ccatatatct ttaaaggaat gaaattgcaa aaagatattg ttacaacaaa
    18721 agaatataac gaggttttta aaaaatggca aataccaaaa agaactagaa aaatacatta
    18781 aataaggaat ggtattgatc ttgataaaat ttttaaaata ctgtcatttt gaatataaag
    18841 gagtttgata tggagtggat tagattaata ggaatagcaa tcattgttgt gggttttatt
    18901 ttaaaatttg atacaattgc aacagtagtc ttagctggtt tggttacagc tttagtttca
    18961 ggtgtttctc tcgttgaatt tttggagatt ttgggaaaag aatttagcaa tcagcgagtg
    19021 ctcacgattt ttatggttac cttgcctctt gtggggctgt cagaaacctt tggactcaag
    19081 caacgatcaa tcgatttgat tcgaaagatt aaaggtctga cagttggaaa cttctataca
    19141 gtttatttct ttattcgaga gttagctggt ttcttttcaa ttcgtctagg aggacaccct
    19201 cagtttgtca gacctttggt tcaacctatg ggagaagcag ctgcagagtc tcaattaggt
    19261 agaaagttaa cagaggttga agatgagaca ataaaagcgc gtgcggctgc gaatgaaaat
    19321 tttggaaatt tctttgctca aaatacgttt gtaggtgctg ggggagtcct cttgataggg
    19381 ggaacattag atcagttagg ctatgaaagc aattatgcag ggattgcttc tacatctatt
    19441 attgttgctg ttataacact tattgtagtg gggatttaca attatttatt tgataaaaaa
    19501 ttgatatcaa aaaagactag gggaggagaa caaaaatgac agagttggca aagcaactat
    19561 tagagttgac ctatattgtg attggttgtc aatttctcca tacagcctat tgtagttata
    19621 aagataaaac aaacccagtt cgacttggga catctgcatt ttggactcta ttgtctatta
    19681 cgtttatagg tggttcctat atgccaaata tgagtattgg tattattgta atcctattat
    19741 cgctgttaac attgtttaag caagtccgta tcggaacctt gccatcctta gatgaaatga
    19801 aagccaatat tgaatctaac aggttgaaaa ataaaatttt tattccagtt atgctgatgg
    19861 caatacttgc gttggtctta gcgcaaatga ttccagaatt tagcaagatt tcgattagcc
    19921 ttgccgcctt gtttgctaca atttctgttc ttgtgattac caatagtcac cctaagagtc
    19981 tgttatcaga aaataatcga atgactcagc aagtttcaac aagtggtatt gttcctcaat
    20041 tattaggggc tttgggggct atttttactg tagcaggtgt tggtgatgtt atctctcatc
    20101 tgattagcgg tattgttcct tcagatagtc gctttatagg agttttggcc tatgttcttg
    20161 gaatggttct attcacaatg attatgggaa atgcttttgc agcattcacc gttattacag
    20221 caggtgttgg agttcccttt gtatttgctc tgggagctaa tccaattgtg gctggtgctc
    20281 ttgccatgac agcaggttat tgtgggacct tattgacccc aatggctgct aattttaacg
    20341 ctctaccagc agcattgatg gatatgaaag atcagaatgg cgttataaag gctcaagcag
    20401 gtgttgctct agtaatgatt gttattcaca tattcttaat gtactttctc gcattttagt
    20461 aaaggaaaat aaaatgaaaa tattggttac aggttttaat ccttttggag gtgaaaagat
    20521 taatccagct ttggaggctg taaaattatt accatctgag attaatgggg ctgaagttcg
    20581 ctgggtagaa attccaacgg ttttttataa gtcgtcagaa gttttagagg cagaaatatt
    20641 acgatatcaa ccagatgctg tactttgtat tggacaagca ggcggcagga ccggcttaac
    20701 acctgaacga gtggctatta atcaagatga tgctcgcata cctgataacg aaggcaatca
    20761 accaattgat acaccgattc gtattgatgg agcatcggcc tattttagta gtttacctat
    20821 caaagcgatg gtacaagcta tcaaaaaaca gggacttccg gcagttgtat ccaatagtgc
    20881 aggaaccttt gtttgcaatc atttgatgta ccaagctctt tatttagtgg ataaaaaatt
    20941 ccctaatatg agagcaggtt tcatgcatat tccatatatg atggaacaag tagtaaataa
    21001 gccgaatact gcaggaatga gtctatgtga tattgtaaga ggcatagagg tagctattga
    21061 agctattgta gactacaaag ataaggattt gcagttagta ggcggtgaga ctcactgata
    21121 acctaaaaag gatagtcaat tatgcttgtt tactaactat taactatgct aaatcaattg
    21181 aggttgttta cataaaactc tatatcagag aagcctgata tagagttttt tcttgctagt
    21241 tttagaattt tcttgtaaaa tagaaaaagt gaagagaggt atgaaatgag caagaaagat
    21301 aaaaaaatcg aaattcaagt agcggatgcc aaagttaatg ttggtaaaga cagttttgaa
    21361 ggttatacat tgactatcgg taaaaaagtt atcggagaaa ttgccgaatt agacggacaa
    21421 tttgccatta taaagaatgg gaatgtcgat agtttttata aaaaattgga aaaagctgtg
    21481 gaaattttga ttgaaaatta taatttagca aaataagtct tgtttttttg aaattttcat
    21541 gatataatag tccatgttga ttgtaggaga gatagcgaag aggctaaacg cggcggactg
    21601 taaatccgct ccttcgggtt cgggggttcg aatccctctc tctccatttc attaatgggg
    21661 tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc gaatccagct
    21721 accccagttc ttaggtaata atcaagataa aaagcaaaat atcttagggt attttatttt
    21781 tataattgaa agacgtgaat gatatgaaca tgtccttgcg ggtgcttagg aaaaaaatta
    21841 taagtatgtc aagtttaaga aaaacttgat tgttggagga ttttttagat gaatgaattt
    21901 gaagatttgc taaatagcgt tagtcaagtt gagactggtg atgttgttag tgctgaagta
    21961 ttgacagttg atgcgactca agctaacgtt gcaatctctg gaactggtgt tgaaggtgtc
    22021 ttgactcttc gcgaattgac aaacgatcgt gatgcagata tcaatgactt tgttaaagta
    22081 ggagaagtat tggatgttct tgtacttcgt caagtagttg gtaaagatac tgatacagtt
    22141 acataccttg tatctaaaaa acgccttgaa gctcgcaaag catgggacaa acttgttggt
    22201 cgcgaagaag aagttgttac tgttaaagga acgcgtgccg ttaaaggtgg actttcagta
    22261 gaatttgaag gtgttcgtgg atttatccca gcttcaatgt tggatactcg tttcgtacgt
    22321 aatgctgagc gttttgtagg tcaagaattt gatactaaaa tcaaagaagt taacgctaaa
    22381 gaaaaccgct tcatcctttc acgtcgtgaa gttgttgaag cagctactgc agcagctcgc
    22441 gctgaagtat tcggtaaatt ggctgttggt gatgttgtaa ctggtaaagt tgctcgtatc
    22501 acaagcttcg gcgctttcgt cgaccttggt ggtgttgacg gattggttca cttgactgaa
    22561 ttgtcacatg aacgtaacgt atcaccaaaa tcagttgtaa ctgttggtga agaaattgaa
    22621 gtgaaaatcc ttgatcttaa cgaagaagaa ggacgtgtat cactttcact taaagcaaca
    22681 gtaccaggac catgggatgg cgttgagcaa aaattggcta aaggtgatgt agtagaagga
    22741 acagttaaac gtttgactga cttcggtgca tttgttgaag tattgccagg tatcgatgga
    22801 cttgttcacg tatcacaaat ttcacacaaa cggattgaaa atccaaaaga agctcttaaa
    22861 gttggtcaag aagttcaagt taaagttctt gaagttaacg cagatgcaga acgcgtgtca
    22921 ctttctatta aagctcttga agaacgtcca gcccaagaag aaggacaaaa agaagaaaaa
    22981 cgtgctgctc gtccacgtcg tccaagacgt caagaaaagc gtgatttcga acttccagaa
    23041 acacaaacag gattttcaat ggctgatttg tttggtgaca tcgaacttta atcaaattga
    23101 aaattcacaa aatcctttgt ttactaaaca agggattttt ctggctcttt gtcaactgta
    23161 gtgggttgaa gaaaagctaa gctcgagaaa ggggctcttt gtcaactgta gtgggttgaa
    23221 gaaaagctaa gctcgagaaa ggggctcttt gtcaactgta gtgggttgaa gt
//
LOCUS       DAVLRT010000024        23172 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLRT010000024 DAVLRT010000000
VERSION     DAVLRT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.024"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            663..2816
                     /locus_tag="VXM85_000950"
     CDS             663..2816
                     /locus_tag="VXM85_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1345553.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLFSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNSLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2829..4178
                     /locus_tag="VXM85_000951"
     CDS             2829..4178
                     /locus_tag="VXM85_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1345554.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQSEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQFFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4348..5055
                     /locus_tag="VXM85_000952"
     CDS             4348..5055
                     /locus_tag="VXM85_000952"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1345555.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIVG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5257..8982
                     /locus_tag="VXM85_000953"
     CDS             5257..8982
                     /locus_tag="VXM85_000953"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1345556.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFTQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVCVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9075..10517
                     /locus_tag="VXM85_000954"
     CDS             9075..10517
                     /locus_tag="VXM85_000954"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1345557.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10554..11576
                     /locus_tag="VXM85_000955"
     CDS             10554..11576
                     /locus_tag="VXM85_000955"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1345558.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11573..12118
                     /gene="purN"
                     /locus_tag="VXM85_000956"
     CDS             11573..12118
                     /gene="purN"
                     /locus_tag="VXM85_000956"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1345559.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12202..12711
                     /locus_tag="VXM85_000957"
     CDS             12202..12711
                     /locus_tag="VXM85_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1345560.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12736..14283
                     /gene="purH"
                     /locus_tag="VXM85_000958"
     CDS             12736..14283
                     /gene="purH"
                     /locus_tag="VXM85_000958"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1345561.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDATYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14405..15667
                     /gene="purD"
                     /locus_tag="VXM85_000959"
     CDS             14405..15667
                     /gene="purD"
                     /locus_tag="VXM85_000959"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1345562.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKTFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15858..16349
                     /gene="purE"
                     /locus_tag="VXM85_000960"
     CDS             15858..16349
                     /gene="purE"
                     /locus_tag="VXM85_000960"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1345563.1"
                     /translation="MTKPIISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTP
                     DLMFKHAEEAYSRGIKVIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSN
                     ELI"
     gene            16336..17427
                     /gene="purK"
                     /locus_tag="VXM85_000961"
     CDS             16336..17427
                     /gene="purK"
                     /locus_tag="VXM85_000961"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1345564.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEVD
                     LEAAYALAESADCVLEEFVNFALEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17437..17664
                     /locus_tag="VXM85_000962"
     CDS             17437..17664
                     /locus_tag="VXM85_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1345565.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            17727..19025
                     /gene="purB"
                     /locus_tag="VXM85_000963"
     CDS             17727..19025
                     /gene="purB"
                     /locus_tag="VXM85_000963"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1345566.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19080..23018)
                     /gene="strH"
                     /locus_tag="VXM85_000964"
     CDS             complement(19080..23018)
                     /gene="strH"
                     /locus_tag="VXM85_000964"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1345567.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKADVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYDDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKIISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAVTHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQEEDIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKVLPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
BASE COUNT         6299 a         4627 c         5542 g         6704 t
ORIGIN      
        1 ttatatggat gatttaatta gaaaaaaatt tttaaagtct tcggagaaga aaacagaaaa
       61 atctgttaaa aaataatcaa aactataaat gatgaatctg aatcaaaatt attttgcgca
      121 tgtaaagagg agtcttatag taacgagtca aaaaaggagt aactatgaat cgtaatttag
      181 aacggtgtta tctattctga ctaggaatag atcataccag aggtagctta gaaatagcag
      241 agacattaga aattgaagta ataaatggga tgtcgtaagt gttactatca atgatttatt
      301 tgtttcaagc ttgcctaggg tgacagtaaa aaatcaattt cctttcaata gcatattttt
      361 agtgggcagg actcttgttc tgcctatttt tttatccaaa aagtgcagtt gggagggaga
      421 taggctcatt tgggaaggaa gtccagtttt tgtttagtga ttggggtaag atagttgtta
      481 tcagatgagt ttatactctt cgaaaatcaa attcaaacca cgtcaacgtc gccttgccgt
      541 atatatgtga ctgacttcgt cagtcctatc tacaacctca aaacagtgtt ttgagcagcc
      601 tgcggctagt ttcctagttt gctctttgat tttcattgag tattagggaa aaggagatga
      661 atatgaaatt tgggaaacgt cactatcgtc cgcaagtgga tcagatggac tgcggtgtag
      721 cttcattagc catggttttt ggctactatg gtagttatta ttttttggct cacttgcgag
      781 aattggctaa gacgaccatg gatgggacga cggctttggg cttggtcaag gtggcagagg
      841 agattggttt tgagacgcga gccattaagg cggatatgac gctttttgac ttgccggatt
      901 tgacttttcc ttttgttgcc catgtgctta aggaagggaa attgctccac tactatgtgg
      961 tgactgggca ggataaggat agcattcata ttgccgatcc agatcccggg gtgaagttga
     1021 ctaaactgcc acgtgagcgt tttgaggaag aatggacagg agtgactctt tttatggcac
     1081 ctagtccaga ctataagcct tataaggaac aaaaaaatgg tctgctctct tttatcccta
     1141 tattagtgaa gcagcgtggc ttgattgcca atatcgtttt ggcaacactc ttggtaaccg
     1201 tgattaacat tgtgggttct tattatctgc agtctatcat tgatacctat gtgccagatc
     1261 agatgcgttc gacactaggg attatttcta ttgggctagt catcgtctac atcctccagc
     1321 aaatcttgtc ttacgctcag gagtatctct tgcttgtttt ggggcaacgc ttgtcgattg
     1381 acgtgatttt gtcctatatc aagcatgttt ttcacctccc tatgtccttc tttgcgacac
     1441 gcaggacagg ggagatcgtg tctcgtttta cagatgctaa cagtatcatc gatgcgctgg
     1501 cttcgaccat cctttcgatt ttcctagatg tgtcaacggt tgtcattatt tcccttgttc
     1561 tattttcaca aaataccaat ctctttttca tgactttatt ggcgcttcct atctacacag
     1621 tgattatctt tgcctttatg aagccgtttg aaaagatgaa tcgggatacc atggaagcca
     1681 atgcggttct gttttcttct atcattgagg acatcaacgg tattgagact atcaagtcct
     1741 tgaccagtga aagtcagcgt taccaaaaaa ttgacaagga atttgtggat tatctgaaga
     1801 aatcctttac ctatagtcga gcagagagtc agcaaaaggc tctgaaaaag gttgcccatc
     1861 tcttgcttaa tgtcggcatt ctctggatgg gggctgttct ggtcatggat ggcaagatga
     1921 gtttggggca gttgattacc tataatacct tgctggttta ctttaccaat tctttggaaa
     1981 atatcatcaa tctgcaaacc aagcttcaga cagcgcaggt tgccaataac cgtctaaatg
     2041 aagtgtatct agtagcttct gagtttgagg agaagaaaac agttgaggat ttgagcttga
     2101 tgaagggaga tatgaccttc aagcaggttc attacaagta tggctatggt cgagacgtct
     2161 tgtcggatat caatttaacc gttccccaag ggtctaaggt ggcttttgtg gggatttcag
     2221 ggtcaggtaa gacgactttg gccaagatga tggttaattt ttacgaccca agtcaagggg
     2281 agattagtct gggtagtgtc aatctcaatc agattgataa aaaagccctg cgccagtaca
     2341 tcaactatct gcctcaacag ccctatgtct ttaacggaac gattttggag aatcttcttt
     2401 tgggagccaa ggaggggacg acacaggaag atatcttacg ggcggtcgaa ttggcagaga
     2461 ttcgagagga tatcgagcgc atgccactga attatcagac agaattgact tcggatgggg
     2521 cagggatttc aggtggtcaa cgtcagagaa tcgctttggc gcgtgctctc ttgacagatg
     2581 cgccggtctt gattttggat gaggcgacta gcagtttgga tattttgaca gagaagcgga
     2641 ttgtcgataa tctcatagct ttggacaaga ccttgatttt cattgctcac cgcttgacta
     2701 ttgctgagcg gacagagaag gtagttgtct tggatcaggg caagattgtc gaagaaggaa
     2761 agcatgctga tttgcttgca cagggtggct tttacgccca tttggtcaat agctagaaag
     2821 aggagaggat gaaaccagaa tttttagaaa gtgcggagtt ttataatcgt cgttaccata
     2881 atttttccag tagtgtgatt gtacccatgg cccttctgct cgtgttttta cttggctttg
     2941 caactgttgc agagaaggag atgagtttgt ccactagagc tactgtcgaa cccagtcgta
     3001 tccttgcaaa tatccagtca actagcaaca atcgtattct tgtcaatcat ttggaagaaa
     3061 ataagctggt taagaagggg gatcttttgg ttcaatacca agaaggggca gagggtgtcc
     3121 aagcggagtc ctatgccagt cagttggaca tgctaaagga tcaaaaaaag caattggagt
     3181 atctgcaaaa gagcctgcaa gaaggggaga accactttcc agaggaggat aagtttggct
     3241 accaagccac ctttcgcgac tacatcagtc aagcaggcag tcttagggct agtacatcgc
     3301 aacaaaatga gaccatcgcg tcccagaatg cagcagctag ccaaacccaa tccgaaatcg
     3361 gcaacctcat cagtcaaaca gaggctaaaa ttcgcgatta ccagacagct aagtcagcta
     3421 ttgaaacagg tgcttccttg gccggtcaga atctagccta ctctctttac cagtcctaca
     3481 agtctcaggg cgaggaaaat ccccaaacta aggttcaggc agttgcacag gttgaagcac
     3541 agatttctca gttagaatct agtcttgcta cttaccgtgt ccagtatgca ggttcaggta
     3601 cccagcaagc ctatgcgtca gggttaagca gtcaattgga atcccttaaa tcccaacact
     3661 tggcaaaggt tggtcaggaa ttgacccttc tagcccagaa aatcttggag gcagagtcag
     3721 gtaagaaggt acagggaaat cttttagaca aggggaaaat tacggcgagt gaggatgggg
     3781 tgcttcatct taatcctgag accagtgatt ctagcatggt tgcagaaggt gccctactag
     3841 cccaacttta tccatctttg gaaagagaag ggaaagccaa actcacagcc tatctaagtt
     3901 caaaagatgt agcaagaatc aaggtcggtg attctgttcg ctatactacg actcatgatg
     3961 ccgggaatca attttttcta gattctacta ttacaagtat tgatgcgaca gctactaaga
     4021 ctgagaaagg gaatttcttt aaaatcgagg cggagactaa tctaacttcg gagcaggctg
     4081 aaaaacttag gtacggggtg gaaggccgtc tacagatgat tacgggcaag aaaagttatc
     4141 tacgttatta tttggatcaa tttttgaaca aagagtaatg ttcgtgtttt tagagttaaa
     4201 taatttttaa actgtgagaa agattcttct tgcagttttt tctttacaat ttttgaaaaa
     4261 catatgctat ttattcggtt aaattcttgt gttttttggt tttttgtggt aaaatgtgct
     4321 caagtaatac gaaaggcgaa ctttaaaatg tcaaaacaat tgatctattc gggaaaagct
     4381 aaagatatct atacaactga ggatgaaaat cttattattt caacttacaa ggaccaggcg
     4441 actgctttca acggtgtcaa gaaggagcag attgtaggta agggagtctt gaataatcag
     4501 atctcatctt ttatttttga gaaattaaat gcggctggtg tggcgactca ctttgtggag
     4561 aaactttcag acacggaaca actcaataaa aaggttaaga ttattccttt ggaagtcgtg
     4621 ctccgcaact atactgctgg ttccttttca aaacgttttg gtgtggatga gggaatcgcc
     4681 ttggagactc cgattgtcga attttactac aaaaatgatg atttggatga tccatttatc
     4741 aatgatgagc atgtgaaatt cctacagatt gcgggtgacc agcagattgc ctacttgaag
     4801 gaagaaacgc gtcgtatcaa tgaactattg aaagtctggt ttgctgagat tgggcttaaa
     4861 ttgattgact ttaagctaga gttcggtttt gacaaggatg gcaagattat cttggcagac
     4921 gaattttcac cagataactg ccgcttgtgg gacgctgatg gcaaccacat ggataaggat
     4981 gttttccgta gaggattggg agaactaacc gacgtttatg agattgtttg ggaaaagttg
     5041 caggaattga aataatctgt ttgcaacgga aaaccttcgt ctctcaacta aaaggactca
     5101 ggctgaaaag gtcccccaga ccttttcact ctgtagagaa ctaggtgaac taacagatgt
     5161 ttacgaaatt gtctgggaaa agttgcaggg tttaaaataa caacctcaag gctgtttggg
     5221 aatattgcaa gagctgaaat aaaggaataa gaattgatgg ataaacgtat ttttgttgaa
     5281 aaaaaggctg attttcaggt caagtcagag agtttggtta gagagctcca gcacaacttg
     5341 ggactgtcaa gcttgaaaag tattcgtatt gtgcaagtat atgatgtatt tgacttggct
     5401 gaggacttgt ttgcacctgc agagaagcac attttctctg agcagggaac cgaccatgtt
     5461 ttagatgaag tatctgtgca ggcggatctt gctaactatg ctttctttgc cattgaaagt
     5521 ctgccagggc agtttgacca gcgtgcagct tcgtcacagg aagccttgct tttgttggga
     5581 agttcgagtg acgtgacagt caacacagcc caactttact tggtgaataa agatattgat
     5641 gcgactgagt tggaagctgt caaaaactac ctgctcaatc cagttgattc tcgtttcaag
     5701 gatatcacga cagggattgc caagcaggag ttttcagagt cagacaagac cattccaaaa
     5761 ttgactttct ttgaaagcta tacagcagaa gactttgctc gctacaaggc cgagcaaggg
     5821 atggccatgg aagtggatga tttgctcttt atccaagact actttaagtc aatcgggcgc
     5881 gtgccaactg agactgaact caaggttttg gacacttact ggtctgacca ctgccgtcat
     5941 acgacttttg agacagagtt gaaacacatc gacttttcag cttctaaatt tcaaaagcaa
     6001 ttgcagtcaa cctatgacaa gtatattgcc atgcgcgagg agttaggtcg gtctgaaaaa
     6061 ccacaaacct tgatggatat ggcgactatt ttcggtcgtt atgagcgtgc taatggacga
     6121 ttggatgata tggaagtctc tgacgaaatc aatgcctgct cagttgaaat tgaagtggat
     6181 gttgatggtg tcaaggaacc ttggctcctc atgtttaaaa atgaaaccca caaccatcca
     6241 acagaaattg agccatttgg tggagcggct acctgtatcg gtggagctat tcgtgatccg
     6301 ttgtcaggcc gttcctatgt ttaccaagcc atgcgtattt caggtgctgg tgatattaca
     6361 gcaccgattt cggaaactcg tgctgggaaa ttgccacaac aagtcatttc taaaacagca
     6421 gctcatggtt attcttcata tggtaaccag attgggcttg ccacaaccta cgttcgtgaa
     6481 tacttccacc caggctttgt agctaaacgt atggaacttg gtgccgttgt tggtgcggct
     6541 cccaagggca atgttgtccg tgaaaaacct gaagcaggtg atgtgatcat ccttctcgga
     6601 ggcaaaacag gtcgtgatgg tgtcggtggt gcgacgggct cttctaaggt tcaaacagtt
     6661 gagtctgtag agactgctgg tgctgaggtt caaaaaggaa atgccatcga agaacgcaag
     6721 attcagcgcc tcttccgtaa tggcaatgtc actcgtctga tcaagaagtc caatgacttt
     6781 ggggcaggcg gcgtctgtgt ggctatcggt gaattggcag acggtcttga aatcgacctc
     6841 aacaaggtgc ctcttaaata ccagggcttg aatggtacag aaattgccat ctctgaatca
     6901 caagaacgga tggcggtcgt ggttcgtcct gaagatgtgg ataccttcgt tgccgaatgt
     6961 aacaaagaaa atattgatgc tgttgtggtg gcgacagtaa ctgaaaaacc aaatcttgtc
     7021 atgcactgga atggtgagac aatcgttgac ttggagcgtc gtttccttga caccaatggt
     7081 gtgcgcgtgg ttgtcgatgc caaagttgtg gacaaggatg tcaaactccc agaagagcgt
     7141 caaacatctg ctgaaacact ggaatcagat acccttacgg ttctatctga cctcaaccat
     7201 gcaagtcaaa aaggattaca gactatcttt gactgctctg ttggacgctc aacggttaat
     7261 cacccacttg gtggtcgtta ccaactcaca ccaactgagg catctgtgca gaaattgcca
     7321 gttcaacacg gtgtgactca tactgcgtcg gtcattgctc aaggtttcaa cccatatgta
     7381 gctgaatggt ctccatacca cggtgctgct tatgcggtta tcgaagcaac tgctcgtttg
     7441 gtggctgctg gtgccaactg gtccaaggct cgtttctctt accaagagta tttcgagcgt
     7501 atggataaac aagcagagcg tttcggtcag ccagtagctg ctcttctagg ttctattgaa
     7561 gcacaaattc agcttggctt gccatctatc ggtggtaagg actccatgtc tggtaccttt
     7621 gaagaattga ccgttccgcc aaccttggtt gcctttgggg tgacgacggc agatagccgt
     7681 aaggtgctct ctccagaatt taaagctgtt ggggaaaata tctactacat cccaggtcaa
     7741 gccctctctg cagagattga ttttgacttg attaagaaaa attttactca gtttgaagcc
     7801 atccaagctg accataaagt gacatctgca tcagctgtca aatacggtgg tgtagttgaa
     7861 agtttggctc ttgctacctt tggaaactat attggtgcag aggtgacctt gcctgaactt
     7921 aaaacagctt tgacagctca attaggcggc tttgtcttca catctcctga agaaattgct
     7981 ggagtagaga aggttggaca aacgaaagca gactttacac tgactgtcaa cggtgtgaag
     8041 ctagatggac acaagcttga cagtgcattt caagggacat tggaagaagt ttacccaaca
     8101 gaatttaccc aagcgaaaga actagaagaa gtaccagctg tggcatcaga tgttgtgatt
     8161 aaagccaaag aaaaggttga aaaacctgtg gtttacatcc cagtctttcc aggaaccaac
     8221 tcagaatatg attcagctaa ggccttcgaa aaagaaggtg cagaggtcaa tttggtgcca
     8281 ttcgtgacct tgaatgaaga agctattgtc aagtcagttg aaactatggt tgacaatatc
     8341 gacaagacta atattctctt ctttgctggt ggattctcgg ctgcggatga accagatggt
     8401 tcagctaagt ttatcgtcaa tatcctgctt aatgaaaaag tgtgtgtggc tattgatagc
     8461 tttatcgccc gtggtggttt gattatcggt atttgtaatg gattccaagc cttagtcaaa
     8521 tcgggtctcc taccctacgg aaactttgaa gctgctaaca gtactagccc aaccctcttc
     8581 tacaatgatg ccaaccaaca cgtggccaag atggtggaaa ctcgcattgc caataccaac
     8641 tcaccatggt tggctggtgt gcaagtgggc gatatccacg ctattcctgt ttcgcacggt
     8701 gaagggaagt ttgtcgtgac ggctgaggaa tttgcagagc tccgtgacaa tggacaaatt
     8761 ttcagccaat acgttgactt taacggtaaa ccaagtatgg attctaagta caatccgaat
     8821 ggttctgtcc atgccatcga aggaattacc agcaagaatg gtcaaatcat cggtaagatg
     8881 ggccactcag aacgttatga ggatggtctt ttccaaaata tcccaggcaa taaagaccaa
     8941 cacctgttcg catcagcggt taaacatttc actggaaaat aagacttaca gattttctaa
     9001 tagatagtat cagtaatgta aaagtcatgt aaatctagct cttgatgatt acaaatgaaa
     9061 attaggtata aaaaatgaca tacgaagtaa aatctcttaa tgaagaatgt ggtgttttcg
     9121 gtatttgggg acatccagat gctgctaagt tgacctattt tggactccac agtcttcaac
     9181 accgtggtca ggagggggca ggaatcctct ccaatgatca aggacaactg aagcgccatc
     9241 gtgacatggg gcttttatca gaagttttca gaaatccagc caatttggat aaattgacag
     9301 gagctggtgc gattgggcat gtgcgttatg cgactgctgg cgaagcttct gttgataaca
     9361 tccaaccctt cctcttccgt tttcacgata tgcagtttgg tttggctcat aatggaaatc
     9421 tgaccaatgc agcctctctc aagaaagaac tggaacaaag aggagcaatt ttcagcgcga
     9481 cttcggactc tgaaatcttg gctcacctca ttcgtcgcag tcataatcct agcctgatgg
     9541 gcaaaatcaa ggaagcgctc agccttgtca aaggtggttt tgcctatatc ttgctgtttg
     9601 aggacaagtt gattgcggct cttgacccta atggctttcg tccgctttct atcgggaaaa
     9661 tggccaacgg agcggtggtt gtttcctctg aaacctgtgc ttttgaggtc attggtgccg
     9721 agtggattcg tgatttgaag ccaggtgaga ttgtgatcat tgatgacgag ggcattcagt
     9781 atgacagcta tacagatgat acccagttgg cgatttgttc tatggagtat atctactttg
     9841 cccgccctga ttctaatatc cacggtgtca atgtccatac ggcacgtaag agaatgggag
     9901 cgcaattggc gcgagaattt aagcatgagg cagatattgt agttggtgtg cccaattctt
     9961 ccctaagcgc ggctatggga tttgcggaag aatcaggctt accaaatgaa atgggtctga
    10021 tcaaaaacca atacacccag cgaactttta tccaaccgac tcaagaattg cgggagcaag
    10081 gagtgcggat gaaactgtct gctgtttcgg gtgttgtcaa aggcaaacgt gtggttatgg
    10141 tggatgattc cattgtacgt ggaacaacct ctcgtcgtat cgttcagctc ttgaaagaag
    10201 cgggtgcgac tgaggttcac gttgccattg gaagtcctgc actagcgtat ccatgtttct
    10261 acgggattga tatccagacc cgtcaggagc tgattgcagc caatcatacg gtcgaagaaa
    10321 ctcgccaaat cattggtgcg gacagtctga cttatctttc aattgatagc ttgattgagt
    10381 cgattggtat cgaaacagat gcgccgaacg gtggtctctg tgtcgcttac tttgacggtg
    10441 actacccaac gcctctctat gactacgaag aagactatcg tagaagtttg gaagaaaaga
    10501 ccagttttta caagtaggcg acagattctc cattaaagaa aaggaaaaaa caaatgacaa
    10561 ataaaaatgc atatgcccag tcgggtgtgg atgttgaagc gggttatgaa gttgttgaac
    10621 ggattaaaaa gcacgtggcc cgtacggagc gtgcaggtgt catgggagct cttggtggct
    10681 ttggtggtat gtttgacctt tccaagactg gggttaaaga acccgtcttg atttcaggga
    10741 ctgacggtgt cggaaccaag ctcatgttgg ctatcaagta cgacaagcac gataccatcg
    10801 ggcaggactg tgtggccatg tgtgtcaacg acatcattgc tgcaggtgcg gaacccctct
    10861 attttctcga ctacgtagcg acagggaaga atgaaccagc taagctagaa caagtggttg
    10921 ctggtgtggc agaaggttgt gtgcaggctg gtgctgccct catcggtggg gaaacggctg
    10981 aaatgccggg catgtacggc gaagacgact atgacttggc tggttttgcg gtcggtgtgg
    11041 ctgaaaaatc tcaaatcatt gacggttcaa aggtggtaga gggagatgtt cttctcggac
    11101 ttgcttcaag tgggattcac tcaaatggtt actctttggt tcgtcgtgtc tttgcggatt
    11161 acacaggtga ggaagtccta ccagaattgg aaggcaagaa acttaaggaa gttctacttg
    11221 agccgactcg tatctatgtc aaggctgtct tgccgctcat caaagaagag ttggtcaacg
    11281 gcattgccca catcacaggt ggtggcttta tcgaaaatgt ccctcgtatg tttgcagatg
    11341 acctagctgc tgaaattgat gaaagcaaag ttccagtgct tccaattttc aaagcccttg
    11401 aaaaatatgg tcagattaaa cacgaagaaa tgtttgaaat cttcaatatg ggtgtgggac
    11461 ttatgttggc ggtcagccct gaaaatgtag agcgtgtaaa agaattgttg gatgaagcag
    11521 tctatgaaat tggtcgcatc gtcaagaaag aaaacgaaag tgtcattatc aaatgaaaaa
    11581 aatagcggtt tttgcctctg gtaatggctc aaattttcag gtgattgccg aagaatttcc
    11641 agtggagttt gtcttttcag accatcgtga tgcctatgtg cttgagcgtg caaagcagct
    11701 cggcgttctg tcctatgctt ttgaactcaa ggagtttgag agcaaggcag actacgaagc
    11761 agcccttgtc gaactcttgg aagaacacca gattgacttg gtttgcctag caggctacat
    11821 gaaaatcgtt ggaccaacct tattgtcggc ttatgaaggt cggattgtca acattcatcc
    11881 agcctacttg ccagaatttc caggagctca tgggattgag gatgcttgga atgctggcgt
    11941 gggtcagtct ggtgtgacca ttcactgggt ggattcgggt gtggatacag gccaggtcat
    12001 caaacaggtt cgtgtgccac gactagctga tgataccatt gacagatttg aagctcgcat
    12061 ccatgaagca gagtacaggc tgtatccgga agtagtgaag gctctattta cagattgact
    12121 ttttgatgat tcatatgata tctttgattt taaattggag tcagtgtttg ttgaagacgg
    12181 cttcaaacgg aggtatttgt aatattagaa tctaaaaaaa caactcgata tgtattttat
    12241 gtctatctga tgttattaac ttggggaatc ttatttaagt ttgaaacaaa tcctgaattt
    12301 atagcatttt tcttagctcc aaggtatatc aattggattc cattttcaga accactaata
    12361 gtcgatggaa aaattgtttt tgctgaaatg ttatttaatc tgatttcctt tattccatta
    12421 ggtgtttgtt tccctttgat aaaaactaat ttatctagtt taagaatagt cgggacaggt
    12481 ttcttgatta gtttattgtt tgagtgctta cagtatattt tagcaatagg tataacagat
    12541 ataacggatt tgactttaaa tacgctaggt gtctgtgtag gcttactgat ttatcaaatt
    12601 tttataagag tgttcaaatc acagactaga aaatggatca atatcttagg tatgcttagc
    12661 cttggttttg cttatcttgt tttactgtta ctgcatttaa ttggtgttta actaatgatt
    12721 aaaaaggaga atataatgac taaacgcgtc ttaatcagcg tctcagacaa agcgggcatt
    12781 gttgaatttg cccaagaact caaaaaactt ggttgggaga ttatctcgac aggtggaact
    12841 aaggttgccc ttgataatgc tggggtggat accattgcta tcgatgatgt gactggtttc
    12901 ccagaaatga tggacggtcg tgtgaagacc ctccacccaa atatccacgg ggggcttctc
    12961 gctcgtcgtg acttggatag ccacttggaa gcggctaagg acaacaagat tgagctcatt
    13021 gaccttgtgg tggtcaacct ttacccattt aaggaaacta tccttaaacc agatgcgact
    13081 tatgctgatg cagttgaaaa tatcgatatt ggtgggccat ctatgcttcg ttcagcagcg
    13141 aaaaatcatg ccagtgttac agttgtggta gatcctgctg actacgctgt ggttttggac
    13201 gaattggcag caaacggcga aacctcttat gaaactcgcc aacgtttagc agccaaagta
    13261 tttcgtcaca cagcggctta tgacgccttg attgcagaat acttcacagc tcaagtgggt
    13321 gaaagcaagc ctgaaaaact cactttgact tatgacctca agcaaccaat gcgttacggt
    13381 gagaatcctc aacaagacgc ggacttttac cagaaagctt tgcctacaga ctactccatt
    13441 gcttcagcca aacagctcaa cgggaaagaa ttgtcattta ataatatccg tgatgcagat
    13501 gctgctatcc gtatcatccg tgacttcaaa gatagtccaa ccgttgtggc tctcaaacac
    13561 atgaatccat gtggaattgg tcaagctgat gacatcgaga ctgcttggga ctacgcttat
    13621 gagtctgacc cagtgtctat ctttggtggg attgtcgtcc tcaaccgtga ggtggatgct
    13681 gcgacagctg agaagatgca cggcgttttc ctcgaaatca tcattgcacc aagctatacg
    13741 gatgaagcgc tagccatttt gatcaataaa aagaaaaact tgcgtatcct tgccttgcca
    13801 tttaatgctc aagaggctag cgaagtggaa gcagaataca caggtgtagt cggtggactt
    13861 ctcgtgcaaa atcaagacgt ggtcaaggaa agcccagctg actggcaagt ggtgaccaaa
    13921 cgtcagccaa ccgagacaga agcgactgct cttgagttcg cttggaaggc tatcaagtac
    13981 gtcaaatcaa atggtattat cgtgaccaac gaccacatga cacttggtgt tggtccaggt
    14041 caaaccaacc gtgtggcttc tgttcgcctt gccattgacc aagccaaaga tcgtctggac
    14101 ggggcggtcc ttgcttcaga tgccttcttc ccatttgcgg ataacgtgga agaaatcgct
    14161 aaagcaggaa ttaaggccat catccagccc ggtggctctg tccgtgacca agaatccatc
    14221 gaagcagcgg ataaatacgg cttgactatg gtctttacag gtgtgagaca ttttagacat
    14281 taagaagata aaagggaaga aaacagtttc tttccttttt tggcttaaaa tgctaactga
    14341 aacaagatta aaacgaactt ttttgatata atattggtaa ataattcgca aaagaggttg
    14401 aggaatgaaa ctgcttgttg tcggttcggg tggtcgtgag catgcgattg ctaaaaagtt
    14461 acttgaatca aaagatgtgg aaaaagtctt tgtagctcct gggaatgatg ggatgactct
    14521 ggatggtttg gaattggtaa atatctctat ttccgaacat tctaaattga ttgacttcgc
    14581 aaagaccaat gatgttgctt ggacctttat cggtccagat gatgcccttg ctgctggtat
    14641 cgtggatgat tttaaccaag ctgggcttaa gacctttggt ccgactaggg ctgcagcgga
    14701 gctggagtgg tccaaggatt ttgccaagga aatcatggtc aaatatggtg tttcgacagc
    14761 agcctatggc acattttcag atttcgagga agcaaaggct tatatcgaaa agcatggtgc
    14821 gcctatcgta gtcaaggcgg atggcttggc gcttgggaag ggtgtcgtcg ttgctgagac
    14881 ggttgagcaa gcggtcgaag ccgctcatga gatgcttttg gacaataaat ttggtgactc
    14941 aggtgcgcgc gtggttattg aggaattcct tgaaggagag gaattttcac tctttgcctt
    15001 tgtcaatggt gataagttct acatcatgcc aacggctcag gaccacaaac gtgcctacga
    15061 tggcgacaaa gggcctaaca cgggtggtat gggtgcctat gcgccagttc cacacttacc
    15121 acagagtgta gttgatacag cggttgacac cattgtcaag ccagttctag aagggatgat
    15181 taaagaaggt cgcccttatc tgggagttct ttacgcaggg cttatcctga cagctgatgg
    15241 accgaaagtc atcgagttca acgctcggtt cggagatcca gaaactcaga ttatcttgcc
    15301 tcgcttaacc tctgactttg ctcaaaatat cacagatatc ctggatagca aggagccaaa
    15361 tatcatgtgg acggacaagg gtgtgactct gggtgtggtt gtcgcatcca agggctaccc
    15421 gctagactat gaaaggggcg ttgagttgcc agccaagaca gaaggcgatg tcatcaccta
    15481 ctatgcaggg gctaagtttg cggaaaatag cagagcactg ctctcaaacg gcggacgagt
    15541 ttatatgctc gttaccacag cagataccgt caaagaagcc caagccagca tctaccaaga
    15601 actatcccaa caaaaaatag aaggactctt ctaccgaaca gatatcggaa gcaaggcaat
    15661 taagtaaaga tataagaata acgcgccgta gtcgccaaac acgataatgg tcgtcgtggt
    15721 gaaaagacca gaacagtgaa tgttctggtc agggggaaat ttggagaccg taggctcaaa
    15781 gtttaggaat gaaaccgaag gtttgcttcc gcctccatca cctaagatca ttattaaaaa
    15841 agaagaaaag gaaaattatg accaaaccaa ttatttccat catcatgggc tcaaaatccg
    15901 attgggcaac catgcaaaaa acagcagaag tcttagaccg cttcggtgta gcctacgaaa
    15961 agaaagttgt ttctgcccac cgtacacctg atctcatgtt caaacatgca gaagaagcat
    16021 atagccgtgg tattaaggtc atcatcgcag gtgctggagg cgcagcccac ttgccaggaa
    16081 tggtagctgc caaaacaacc cttcctgtca taggggtacc tgtcaagtct cgtgctctta
    16141 gtggagtgga ttcactctat tctatcgttc agatgccggg tggggtgcct gttgcgacca
    16201 tggctatcgg tgaagctgga gcgactaacg cagctctctt tgccctccgt ctcctctctg
    16261 tagaagataa gtccattgcg gatgcacttg ccaactttgc tgaagaacaa ggaaaaatcg
    16321 cagaggagtc gtcaaatgag ctcatctaaa acaatcggaa ttatcggtgg cggtcaactg
    16381 ggtcagatga tggccatttc tgctatctac atgggccaca aggttatcgc gctggatcct
    16441 gcggcggatt gcccggtctc tcgtgtggcg gaaatcattg tggcacctta taacgatgta
    16501 gacgccctcc gtcagttggc agaccgttgc gatgtcctca cttacgagtt tgaaaatgtc
    16561 gacgctgacg gtttggatgc cgttatcaag gatggacaac tccctcaagg gacagacctg
    16621 ctccgcattt cgcaaaatcg tatttttgaa aaggactttt tgtcaaacaa ggctcaagtc
    16681 actgtggcac cctacaaggt cgtgacttct agcctagact tggcagatat cgacttgtcg
    16741 aaaaactatg tcctcaagac tgcgactggt ggctacgatg gtcatggaca aaaggttatt
    16801 cgttcagaag tagacttgga agcagcctat gcgctagcag aatcagcaga ctgcgtcttg
    16861 gaagaatttg tcaactttgc ccttgagatt tctgtcatcg tgtcaggaaa tggcaaggag
    16921 gtgacgtttt tcccagttca ggaaaatatc caccgcaaca atatcctgtc taagaccatc
    16981 gtaccagccc gcatttctga aagtctagta gacaaggcta aagctatggc agtgcgaatc
    17041 gcagaacaac tcaacttgtc tggaactctc tgtgtggaaa tgtttgcgac tgatgatgac
    17101 gttattgtca atgaaatcgc cccacgacca cataactctg ggcactattc tattgaagcc
    17161 tgtgatttct ctcagtttga cacccatatt ctcggtgttc tcggagcacc attaccagtc
    17221 atcaaactcc atgcgccagc cgttatgctc aatgtcctag gtcaacacgt cgaggctgct
    17281 gaaaagtatg tcgcagaaaa tccaagcgcc cacctccaca tgtatggtaa aatagaagca
    17341 aagcataatc gtaagatggg acatgtgacc ttgtttagtg atgtaccgga tagtgtggat
    17401 gagcttgggg aagggattga tttttaggac aagtctatga tacaaattat cgttaataca
    17461 tttattgaaa aggataagac tggagcagtt gttgaagtgt tgtatgccag tgctgaccaa
    17521 gataaggtac aagctaaata tgaagaacta gctgcacaat accccgaaaa ttatttagct
    17581 atctatgatg taccgctgga tacggatttg aatacactag atcattaccc gtctgtgttt
    17641 attggaaaag aggagtttga gtagaaatct tggtttacct agatagctta ttcccaacag
    17701 cttaagaaga aaggaaaaat taacacatga tcaaccgtta ctctcgccct gagatggcga
    17761 atatttggag tgaagaaaat aaataccgtg cttggcttga ggtggaaatc ctctctgacg
    17821 aggcatgggc tgagttgggg gaaatcccta aggaagatgt ggctttgatt cgcaagaagg
    17881 cggactttga catcgaccgt attttggaaa ttgagcagga gacgcgccac gatgtggtgg
    17941 ctttcacgcg tgcggtttct gagactcttg gtgaagagcg caagtgggtt cactatgggt
    18001 taacttctac cgacgtggtg gatactgctt atggttacct ctacaagcag gccaacgaca
    18061 tcatccgtcg tgaccttgaa aacttcacta atatcatcgc tgacaaggcc aaggagcaca
    18121 agttcaccat catgatgggg cgtactcatg gtgtgcacgc tgagccgaca acctttggtc
    18181 ttaaattggc aacttggtac agcgaaatga aacgcaatat cgagcgcttc gagcatgcgg
    18241 ctgctggtgt agaagctggt aagatttctg gtgcggttgg gaactttgcc aatatcccac
    18301 catttgtaga ggagtatgtc tgcgataaac ttggcatccg tgcccaagaa atctctacac
    18361 aagtccttcc tcgtgacctt cacgctgagt actttgcggt tcttgccagc attgcgactt
    18421 caatcgaacg tatggcgact gagattcgtg gtctacaaaa atctgagcaa cgcgaagtag
    18481 aagagttctt tgctaaaggg caaaaagggt cttcagcaat gcctcacaaa cgcaacccaa
    18541 tcggttctga aaatatgact ggtctggcgc gtgtcattcg tggtcacatg attacggctt
    18601 atgaaaacgt cgctctctgg cacgaacgcg atatttctca ctcatcagct gagcgtatca
    18661 tcacaccaga tacgaccatt ttgattgact acatgctcaa ccgttttgga aatatcgtca
    18721 agaacttgac agtcttccca gaaaatatga tccgaaacat gaactcgact tttggtctta
    18781 tctttagcca acgggctatg ttgacattga ttgaaaaagg catgacccgt gagcaagcct
    18841 atgacttggt gcaaccaaaa acagcctact cttgggacaa ccaagtagac tttaaaccac
    18901 ttcttgaggc agattcagaa gtaacatcac gtctcacaca agaagaaatc gatgaaatct
    18961 tcaacccagt ttattacacc aaacgagtgg atgatatctt tgaacgtctt ggactaggtg
    19021 attaattaaa aaataaacag cgagcttcaa tctcgctgtt tattttttat cgaaaagact
    19081 tagtcttctt ttcttttagt gagtccatag gctgctagtg tggacatgag tcctgcgact
    19141 actagtcctg cagaatcgtg agttcctgtt tcaggaagtt ttttctctgt taccacagga
    19201 gctggatctt gaggaagaac tttgctttcc tcagcaggag cagttgatgg agctggttgg
    19261 cttgggattt ctagttttgg tttttcttca gcaatagcgg cttgtccgtt ttcatcgcct
    19321 acatgtgtta ccatagttcc gacttcgact atttgagtaa cggcttcctg tgctacgaca
    19381 ctatttacaa gtgttttcac ttccttacca tcggcagaag tgctcacaga gtagaagttg
    19441 ctacgatgtc cattgacgcc cttagtaatg acttgtgttt ttcctttgag taagagtgga
    19501 ttttcacgag tcactgtggt aaatggaatg tcttcttctt ggatatccag tctaggtttt
    19561 acctcagtag ttggtgcaag accactttca tcacccttgt gagttacagg agcgccaact
    19621 tcaaccactt ggtttataac ttctttggtt acctggctat caaggactgt ttctgttgtt
    19681 tttccatttt cagtgagtac agagatgtaa tgagttcgtt cacctttgac tcctgctgtg
    19741 ataatatttt cctgaccggc tgggaggtta ggattttctt tcttgataac ttcaaatgga
    19801 atttcttcag ttcttgtgat gagttctggt ctggtttcaa cattggcagc cacttcattt
    19861 tcatctaggc ttcctgaatg agttacagct ggtttgaggc cttgaagagc ggcttttagg
    19921 ttggctacaa gtgtgtcaag ctcagcttgt ttattacggt tgaggttgta atttagagct
    19981 gttttagctg cgtcaagggc ctcaagactt tctttactat atccttctaa gtttgtagga
    20041 attttagcta attcttcgcg gagagcatta taattagcac ggaagtagtc tttgttgtgg
    20101 tctgcaaagg cagtcatgag ttcaaagatt tcctcttcct tgtattcagc gcttggtcta
    20161 tctgcccaga ttgaaagcat acttccgact gttggaagat ctacttcagg atatttggta
    20221 gaagctagtt gattgaatgg tgtttttcca gtattctcaa tagctttctt gaggaaacca
    20281 ccaccatctt ctggtttttg accaagaatg tagtaccagt caccgttggt attcaagaat
    20341 ttatagcctt tgcttgctag gtattgaggt gatgcgaggt tatatcccca ccagccttta
    20401 gaccagtaag aaatcaagac atctttgtca aactgaacat cgtccttgtc ttcatagtag
    20461 aagccatcgt tgaaggccat tggttgaagc cctctttctt tggccatagc tgcgagggtg
    20521 ttggcatatt cggcaaattt gccatagagt tgataccact tgaggtagta ccagccttgg
    20581 gcactagtcg catcgttggc gtattcgtca gtaccaaagt tgaaaatctt tgttttacct
    20641 gcaaagaagt ccatgtattt accgatgagg gcttttacaa agttcatcgc ttcttcgttt
    20701 ttcaagtcca tagttgtttt tgaaacttta tcaaagtggg cttgaggatt tttaatacct
    20761 aatttttcca tggcaaccag catagcatcc atgtgacctg gactgttaat agctgggatg
    20821 agaccgatgt ccttagattt agcgtattca attagctctg tcacttctgc ctgtgttagt
    20881 gcagtaccgt ttggatcgtc gtagtaagct ttagttcctt cgataatagc ttttttaacg
    20941 tcatcactag cataggtttt tccgttggca gtaatggtca tatcatcgag tagaaagcga
    21001 agtccgtcat ttcctagaag gagatggaca tcagaatatc cgagctcact ggccttgtct
    21061 acgatgcgtt tgagctggtt cagagtaaag tatttgcgtc cagcatcgat tgagattatc
    21121 ttgtttttgg caagtttttc aacctcacgt ttagcttctt cttctttttg agcttcaggc
    21181 gtgagggtca agttgttgac agtttcttga agtttagcaa tggcttgatc aatcgtatct
    21241 tgttgggcac ggctaaggtt gctatcgaga gagcgaatag ctttttcagc ttcttttacg
    21301 gccgtgacgc tttctgcagt ataacggttc aggtcttttg gtacctcgtt aagtgcttgc
    21361 tctgcagatt cataatcagc tgcgaagtat tcagcgttgg catttgcaaa atgacgcatg
    21421 agtttgaaga ggcgtgatgg tgaataacgt gcagatggag tgtcagccca agcagctacc
    21481 ataccaccga tgattgggat atcagctcct tctgtttttg gtacagaagt gattggtgtg
    21541 tttttaatac cattgagccc ctgatcgaga ttgtaccagc cttggccatc agcgtttcgt
    21601 ccaagaacgt agtaccaagc atcattggta ttaaggattt ggtgaccttt ttcagctagt
    21661 agtttagaag aagcgacatc gtagcctccc caaccaccag tccacataga aacgatgatg
    21721 tctttgtcaa aactaccaaa gcttgtgtcg ctattgtagt agataccgtc gttaaaggcc
    21781 attggtttga gaccgtgcga ttttacaata cgagcgaggt cattggcgta ggcaataaat
    21841 ttttcatagc cttttacagg gtagccttcg tttggatagt atttatcagc ttgaagcaca
    21901 ctccaacctt tagcatctgt cgcatcattg gcatattcat caagtccgat gttgaagatt
    21961 tcagtctttt tcgcgaaata agcagcatac ttgtcgataa gggcttttgt aaaagcgaca
    22021 gcttgttcgt tgtcaagatc gacagtacgg gctgattcct tcccaaaata gctaaagtta
    22081 gggttttgga ttcccaattc tttcatggca ttgagaatcg catccatgtg cccaggacta
    22141 tttactgtcg gaatgagacc gataccttta tctttggcat agttaatcag atctgtcatt
    22201 tgactttctg ttaagtgatt gccgtttgga tcgtcgtaat aatcatttgt acctttttca
    22261 atggcgcgtt tgacatcgtc actggcatag gtcttgccgt tagctgtgat gctcatatcg
    22321 tccaacatga aacggagtcc atcatttccg actaataggt gtaaatcagt gtagccataa
    22381 tgtttcgctt tatcgatgat ttccttgagc tgttctggtg agaaatattt acgtccagca
    22441 tcaatagaaa caattttctt tttcgctagt ttttcattta cagttgcagc acgttccttt
    22501 cctgcctctg tttccggttt gtcagcctct gctttcgctt catctttttt agctggttta
    22561 tccttgtcag tcttgtctgt atttgactct ttagaatcaa cctctttcgc ttcttccttt
    22621 ttagggctag cttcttctgc ctttttatta gcagtttctt tttcagcaga agttggagtt
    22681 accacgtctg ctttatcact aggagttgaa ctaacttcct cttgtggttt ttcttctgtt
    22741 tttggaagac tagctacctt atcagtagct ggagtttctg tttctacagt ttttggagct
    22801 tctggttgaa gctctgcttt aggtgtttcc tcagtccgat tttcggatga ttgaggggaa
    22861 tcagaaaccg tatggatggt cggttggttt tctgtagtag taggagtaac tccatcggct
    22921 gcaacagtct gtgcttggaa ggcaaatcca attagaacag aagctgctcc tacagcgtat
    22981 ttacgaatag aaaaacgctg ttgtttttca tgtttcattg caaaacctcc tgattgcatt
    23041 gttatattga tagcgattat ataaatcaac gcctttattt tatttcttat attaacgaga
    23101 gtcaagagga gatgacaaaa aactataata agtataaaaa aatataaaat ttaaacttaa
    23161 gatttcagat tg
//
LOCUS       DAVLRT010000025        22884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLRT010000025 DAVLRT010000000
VERSION     DAVLRT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.025"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            380..727
                     /gene="ssrA"
                     /locus_tag="VXM85_000965"
     tmRNA           380..727
                     /gene="ssrA"
                     /locus_tag="VXM85_000965"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(910..997)
                     /locus_tag="VXM85_000966"
     tRNA            complement(910..997)
                     /locus_tag="VXM85_000966"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(961..963),aa:Ser,seq:gga)
     gene            1159..1899
                     /locus_tag="VXM85_000967"
     CDS             1159..1899
                     /locus_tag="VXM85_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1345568.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPAVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1896..2807
                     /gene="pfkB"
                     /locus_tag="VXM85_000968"
     CDS             1896..2807
                     /gene="pfkB"
                     /locus_tag="VXM85_000968"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1345569.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAELIKETYGKVEVEKR"
     gene            2804..4756
                     /locus_tag="VXM85_000969"
     CDS             2804..4756
                     /locus_tag="VXM85_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1345570.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YVAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4920..7145
                     /locus_tag="VXM85_000970"
     CDS             4920..7145
                     /locus_tag="VXM85_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1345571.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGSSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7190..7618)
                     /locus_tag="VXM85_000971"
     CDS             complement(7190..7618)
                     /locus_tag="VXM85_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345572.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            7756..8898
                     /locus_tag="VXM85_000972"
     CDS             7756..8898
                     /locus_tag="VXM85_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1345573.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHSLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDGVQALTKIPTEKYLTERVDFATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8907..10121
                     /gene="thiI"
                     /locus_tag="VXM85_000973"
     CDS             8907..10121
                     /gene="thiI"
                     /locus_tag="VXM85_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1345574.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            10256..11061
                     /locus_tag="VXM85_000974"
                     /pseudo
     CDS             10256..11061
                     /locus_tag="VXM85_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            11083..11822
                     /locus_tag="VXM85_000975"
                     /pseudo
     CDS             11083..11822
                     /locus_tag="VXM85_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            11835..13001
                     /locus_tag="VXM85_000976"
     CDS             11835..13001
                     /locus_tag="VXM85_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1345575.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            13509..15002
                     /locus_tag="VXM85_000977"
     CDS             13509..15002
                     /locus_tag="VXM85_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1345576.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            15015..16124
                     /locus_tag="VXM85_000978"
     CDS             15015..16124
                     /locus_tag="VXM85_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1345577.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLA
                     LQNEFADFVVQVDKSQFACEIAIKVWRNSLKFSII"
     gene            16154..19439
                     /locus_tag="VXM85_000979"
                     /pseudo
     CDS             16154..19439
                     /locus_tag="VXM85_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            19683..19784
                     /locus_tag="VXM85_000980"
                     /pseudo
     CDS             19683..19784
                     /locus_tag="VXM85_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(20025..20495)
                     /locus_tag="VXM85_000981"
     CDS             complement(20025..20495)
                     /locus_tag="VXM85_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1345578.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(20512..22785)
                     /locus_tag="VXM85_000982"
     CDS             complement(20512..22785)
                     /locus_tag="VXM85_000982"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1345579.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSSQDVIREVVYVESRVDTDQKGLPDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         6921 a         4108 c         5020 g         6835 t
ORIGIN      
        1 ctgtcctgat ctattcgtcc tattcttatt tcattttact atattttggt gcaataagtg
       61 aaaagtagtc cgaataatat aaggattgat tttatagttt ttaaactcaa atgaattaaa
      121 ataaagagag tacgaaaatt ctcatctgaa agtattttag aataattttc ttcgtgaatt
      181 tcttcaaaac agatagcttc atcttaggta tgtgttaatt ggatttcgta agctctattt
      241 acaatcgtaa agttatgcac tgagaaagtt gtagttagtc ttccagtttg ctaaatttct
      301 cgaatagtat cttttttagg tatgtgattt ctttttgcat ttttgagtta gataaggtat
      361 aatggtttta ttgtcttttg gggtcgttac ggattcgaca ggcattatga ggcatatttt
      421 gcgactcgtg tggcgacgta aacgctcagt taaatataac tgcaaaaaat aacacttctt
      481 acgctctagc tgcctaaaaa ccagcaggcg tgacccgatt tggattgctc gtgttcaatg
      541 acaggtctta ttattagcga gatacgatta agccttgtct agcggcttga taagagattg
      601 atagactcgc agtttctaga cttgagttat gtgtcgaggg gctgttaaaa taatacataa
      661 cctatggttg tagacaaata tgttggcagg tgtttggacg tgggttcgac tcccaccggc
      721 tccattattc ctttgcattc ttttgcattc cttggtaaaa cgttgttaaa tcaacgtttt
      781 ttatttttat ctttggtatt cctttgcatt cttttgctaa aaagggagtc acaaacagac
      841 cctattttaa aaaaaggata gaaaaaagga tacaacattt gtcgcatcct aaaaataatc
      901 ttttttccac ggaagacatg ggattcgaac ccacgcacgc tattacacgc ctaccgcgtt
      961 tccaacacgg cctcttaagc ctcttgagta atcttccaat acttactcaa atagtctacc
     1021 ataaaggctc ttatcttgca ataaaaattc tagaaatatg aaaaatgata gattttgaaa
     1081 gaaaatgata aaaaatgctt gacttcgaaa gaaagtatga tagaatgaat agtgtaaacg
     1141 ataacaggag gtgattcagt gttaaaaaca gaacgtaaac aactaatttt agaggagtta
     1201 aatcaacatc atgtagtttc tctagaaaaa ttagttagtt tgctagaaac gtcagaatca
     1261 acggttcgaa gagacttgga tgagttggaa gcggaaaaca agcttcgtcg tgtgcatggt
     1321 ggagcagaac tcccccactc cttacaggaa gaagaaacca ttcaagaaaa atctgtcaaa
     1381 aaccttcaag aaaagaagtt gctggctcag aaagcagcct ctctcattaa agaaaaagat
     1441 gtcatcttta tcgatgctgg aacaacaact gcttttttga ttcatgaatt ggtcaataag
     1501 aatgttacag ttgtgaccaa ctccattcac catgccgctc agttggttga aaagcagatt
     1561 ccagctgtca tggttggagg aaacgtcaag acggcgacag atgctagtat cgggggcgtt
     1621 gctcttaacc agattaacca attgcacttt gaccgtgcct ttatcggaat gaatggtgtt
     1681 gacgatggct attatacgac tcctgatatg gaggagggag ctgtgaaaag agctattttg
     1741 gagaatgcca agcagaccta cgtcttggtg gattcgtcaa aaattggaca aacttgcttt
     1801 gccaaggtag caccactcaa acgcgctatc gttatcacta gtcaagggca tgagctcttg
     1861 caggttatta aggagaaaac ggaggtaata gaagtatgat ttatacagtc acactcaatc
     1921 catccattga ctatatcgtt cgtttggacc aagtcaaagt tggtagtgta aatcgtatgg
     1981 acagtgatga taagtttgct ggtgggaaag gaatcaatgt cagccgtgtc ttgaaacgtt
     2041 tgaatatacc aaatacagcg acgggattta tcggtggctt tactggtaaa tttatcacag
     2101 atactttagc agaggaagaa atcgagacac gttttgtcca ggtggcagaa gatactcgta
     2161 tcaatgttaa aatcaaagca gaccaagaaa cagaaatcaa cggaacgggt ccaactgttg
     2221 aatcggttca gctagaagaa ttgaaagcta ttttatctag tctgacagca gaagatacag
     2281 ttgtctttgc aggttcaagt gctaaaaatc taggcaatgt tatctataag gatttgattt
     2341 ccttgacgcg ccagactggt gcgcaagtgg tctgtgactt tgaaggacag accttaattg
     2401 atagtttgga ctaccagcct cttcttgtaa aaccaaacaa tcatgaactt ggagcgattt
     2461 ttggggttaa actcgaaagt ttagatgaaa ttgagaaata cgctcgtgag ttactggcta
     2521 agggtgctca aaatgttatt atctctatgg ctggggacgg tgctcttctt gtcacatctg
     2581 agggagctta ctttgctaaa ccaatcaaag gaacagtcaa aaattcagtt ggggctggtg
     2641 attctatggt tgctggattc acaggtgaat ttgtcaaatc aaaagacgca gtagaagcct
     2701 tcaaatgggg agtggcttgc ggaacggcaa ctaccttctc agatgacttg gcaacggcag
     2761 aacttattaa agaaacatat ggaaaagttg aggtagaaaa acgatgaaaa ttcaagacct
     2821 attgagaaaa gatgtcatgt tgctagattt gcaggcaact gaaaaaacag ctgtcatcga
     2881 cgagatgatt aaaaatttga cagaccacgg ttatgtaaca gattttgaaa catttaaaga
     2941 aggaattttg gcgcgtgaag ctttgacttc tactggtttg ggtgatggaa tcgcaatgcc
     3001 tcacagcaaa aacgctgctg tcaaagaagc gacagttcta tttgctaagt caaataaggg
     3061 tgttgactac gagagcttgg atggacaagc aactgacctc ttcttcatga ttgcagctcc
     3121 agaaggtgcc aatgatactc acttggcagc cttggcagaa ttgtctcaat acttgatgaa
     3181 agacggtttt gcagacaaac ttcgtcaagc aacatctgca gaccaagtta tcgaactttt
     3241 tgaccaagct tcagaaaaaa ctgaggaact tgttcaagca cctgctaatg actctggtga
     3301 ctttatcgta gctgttacag cttgtacaac aggtattgcc cacacttaca tggcccaaga
     3361 agcccttcaa aaagtagctg ctgaaatggg ggttggtatc aaggtcgaaa ccaacggtgc
     3421 tagcggtgtt ggaaatcaac taactgcaga agatatccgt aaggctaaag ctattatcat
     3481 tgcagcagac aaggccgttg aaatggatcg atttgatgga aaaccattga tcaatcgtcc
     3541 agttgctgac ggtatccgta agacagaaga gctaattaac ttggctcttt caggagatac
     3601 tgaagtctac cgtgccgcta atggtgcaaa agctgcaaca gcctctaacg aaaaacaaag
     3661 ccttggtggt gccttctaca aacacttgat gagtggtgta tctcaaatgt taccattcgt
     3721 tatcggtggt ggtatcatga ttgcccttgc cttcttgatt gacggtgctt tgagtgttcc
     3781 aaatgaaaac cttggcaatc ttggttctta ccatgagtta gcttctatgt tcatgaaaat
     3841 tggtggagct gcctttggtt tgatgcttcc agtctttgcg ggttatgttg cctactctat
     3901 tgctgaaaaa ccaggtttgg tagcaggttt cgtggctggt gctattgcca aagaaggttt
     3961 tgcctttggt aaaattcctt atgtcgcagg tggtgaagca acttcaactc ttgcaggtgt
     4021 ctcatctggt ttcctaggtg cccttgttgg tggatttatc gcaggtgcct tggttcttgc
     4081 catcaagaaa tacgttaaag ttcctcgttc actcgaaggt gctaaatcaa tccttctatt
     4141 gccacttctt ggaacaatct tgacaggatt tgttatgcta gctgtgaata tcccaatggc
     4201 tgcaatcaac actgctatga atgacttcct aggtggtctt ggaggaggtt cagctgtcct
     4261 tcttggtatc gtccttggtg gaatgatggc tgttgacatg ggtggaccag ttaataaagc
     4321 agcttatgtc tttggtacag gtacgcttgc agcaactgtt tcttcaggtg gttctgtagc
     4381 catggcagca gttatggctg gaggaatggt gccaccactt gcaatctttg tcgcaactct
     4441 tcttttcaaa gataaattta ctaaggaaga acgtaactct ggtttgacaa acatcatcat
     4501 gggcttgtca tttatcactg agggagcgat tccatttggt gccgctgacc cagctcgtgc
     4561 gattccaagc ttcatccttg gttcagcagt agcaggtgga ctcgttggtc ttactggtat
     4621 caaactcatg gcgccacacg gaggaatctt cgttatcgcc cttacttcaa atgctctcct
     4681 ttacctcgtt tctgtcttgg taggagcaat cgtaagtggt gtggtttatg gttacctacg
     4741 caaaccacaa gcataaaaaa tagaaaaatg aaaagattgg accgtttggt gcagtctttt
     4801 tctcttcccg aaatgcctgt gaaatatggt ataatagaag aatggcaaac aagaatacaa
     4861 gtacaacaag acggagaccg tctaaagcag aactggaaag aaaagaagcg attcaacgaa
     4921 tgttgatttc gttaggaatt gcgattttat tgattttcgc agccttcaaa ttaggggctg
     4981 caggtataac cctttataat ttaattcgct tgctagtggg tagcctagct tatctggcga
     5041 tattcggcct attaatctat ctcttctttt tcaagtggat acgaaaacag gaaggactct
     5101 tatctggttt tttcaccata tttgctggct tactcttgat ttttgaggcc tacttggttt
     5161 ggaaatatgg tttggacaag tccgttctaa aagggaccat ggctcaggtt gtgacagatc
     5221 tgactggttt tcgaacgact agctttgctg gagggggctt gatcggggtc gctctttata
     5281 ttccaacagc ctttctcttt tcaaatatcg gaacttactt tattggttct atcttgattt
     5341 tagtgggttc tctcctagtc agcccttggt ctgtttacga tattgctgaa tttttcagta
     5401 gaggctttgc caaatggtgg gaagggcacg agcgtcgaaa agaggaacgc tttgtcaaac
     5461 aagaagaaaa agctcgccaa aaggctgaga aagaggctag attagaacaa gaagagactg
     5521 aaaaagcctt actcgatttg cctcctgttg atatggaaac gggtgaaatt ctgacagagg
     5581 aagctgttca aaatcttcca cctattccag aagaaaagtg ggtggaacca gaaatcatcc
     5641 tgcctcaagc tgaacttaaa ttccctgaac aggaagatga ctcagatgac gaagatgttc
     5701 aggtcgattt ttcagccaaa gaagcccttg aatacaaact tccaagctta caactctttg
     5761 caccagataa accaaaagat cagtctaaag agaagaaaat tgtcagagaa aatatcaaaa
     5821 tcttagaagc aacctttgct agctttggta ttaaggtaac agttgaacgg gccgaaattg
     5881 ggtcatcagt gaccaagtat gaagtcaagc cggctgttgg tgtaagggtc aaccgcattt
     5941 ccaatctatc agatgacctc gctctagcct tggctgccaa agatgtccgg attgaagcac
     6001 caatccctgg gaaatcccta atcggaattg aagtgcccaa ctccgatatt gccactgtat
     6061 ctttccgaga actatgggaa caatcgcaaa cgaaagcaga aaatttcttg gaaattcctt
     6121 tagggaaggc tgttaatgga accgcaagag cttttgacct ttctaaaatg ccccacttgc
     6181 tagttgcagg ttcaacgggt tcagggaagt cagtagcagt taacggcatt attgctagca
     6241 ttctcatgaa ggcgagacca gatcaagtta aatttatgat ggtcgatccc aagatggttg
     6301 agttatctgt ttacaatgat attccccacc tcttgattcc agtcgtgacc aatccacgta
     6361 aagccagcaa ggctctgcaa aaggttgtgg atgaaatgga aaaccgttat gaactctttg
     6421 ccaaggtggg agttcggaat attgcaggtt ttaatgccaa ggtagaagag ttcaattccc
     6481 agtctgagta caagcaaatt ccgctaccat tcattgtcgt gattgtggat gagttggctg
     6541 acctcatgat ggtggccagc aaggaagtgg aagatgctat catccgtctt gggcagaagg
     6601 cgcgtgctgc aggtatccac atgattcttg caactcagcg tccatctgtt gatgtcatct
     6661 ctggtttgat taaggccaat gttccatctc gtgtagcatt tgcggtttca tcaggaacag
     6721 actcccgtac gattttggat gaaaatggag cagaaaaact tcttggtcga ggagacatgc
     6781 tctttaaacc gattgatgaa aatcatccag ttcgtctcca aggctccttt atctcggatg
     6841 acgatgttga gcgcattgtg aacttcatca aggctcaggc agatgcagac tacgatgaga
     6901 gttttgatcc aggtgaggtt tctgaaaatg aaggagaatt ttcggatgga gatgctggtg
     6961 gtgatccgct ttttgaagaa gctaagtctt tggttatcga aacacagaaa gccagtgcgt
     7021 ctatgattca gcgtcgttta tcagttggat ttaaccgtgc gacccgtctc atggaagaac
     7081 tggagatagc aggtgtcatc ggtccagctg aaggtaccaa acctcgaaaa gtgttacaac
     7141 aataaaaaaa tagcttcttt ccgagtttgg agggaagcta ttttagtggc tattgattgc
     7201 ttttattttc tgaagttggc gcattggact gtttttcgtt ttcagtagca ggtttacttg
     7261 aagcaggagt agaagagtcc tgagttgctg ttttctgatc ttcttttttc tcttccttga
     7321 cgctagattt tggtgtttcc tcttgctgtg ttttttcttg actagtgtta gtctctttag
     7381 ttggactggt gttttcctta ggggattcct tttggatttc tttgacaatg gttgtcgtct
     7441 ggcttgtcgt aggttctttt ttaatatttt tgttattatc caaggcgttc ataatcgtga
     7501 tacttgcggt aatcaagcca aggatactag cgattgtagc aacggttttt tgaaagactg
     7561 taaagccttt tttgatgtgt tctgtttttg gttttgaagt tctacgatag ttagacatac
     7621 attctctcct ttgattatga tttattatac tctgtttctt taaaaaaatc ttaaaaaaag
     7681 agaaattgcc ctttcttgtc gcaagctccg tttcatgata caatagagga agtgatttta
     7741 gaaagcgtga gaaacatgat ttactttgat aattcagcga cgaccaagcc ttatcctgaa
     7801 gcccttgaaa cctatatgca ggtcgcttca aaaattttag gaaacccatc tagtctccat
     7861 agtttgggag accaagcaac acgaatctta gatgcttctc gccaacagat tgcagattta
     7921 atcggtaaga aaagcgatga aatcttcttt acctctggtg gaacagaagg ggataactgg
     7981 gttatcaagg gtgtggcctt tgaaaaagct cagtttggca agcacatcat tgtttcagcc
     8041 attgaacatc cagcagtcaa agagtcagcc ctctggttga aaagtcaagg atttgaagtg
     8101 gattttgctc cagttgataa gaaaggcttg gtcgatgttg aggcgttagc aggtttgata
     8161 cggcctgata caatcctcgt ttctatcatg gctgtgaaca atgaaatcgg ctctatccaa
     8221 cctattgagg ctatttcaga attcttggca gacaagccga ctatttcctt ccacgttgat
     8281 ggggttcagg cgcttaccaa gattccgact gaaaagtatc tgacagaacg agtggatttt
     8341 gcgactttct ctagtcacaa gttccacggg gttcgaggtg ttggctttgt ctatatcaaa
     8401 tctggcaaga agattacacc tcttcttaca ggtggtggcc aggagcgaga ttatcgttcg
     8461 acaactgaaa atgtggcagg gattgcagcg acagccaagg ccctccgttt gtctatggaa
     8521 aagctagata tctttaggag caagactggg cagatgaagg cagtgattcg ccaagctctt
     8581 ctgaactatc cggatatttt tgtcttttca gatgaggaag actttgcacc tcatattctg
     8641 acttttggaa tcaaaggtgt tcgaggtgaa gtcatcgttc acgcctttga agactatgat
     8701 attttcatct caacaacctc agcttgttca tctaaggcag gaaaaccagc cggtaccttg
     8761 attgccatgg gagtggacaa agataaggcc aagtcagctg tgcgtcttag cctagacttg
     8821 gaaaatgata tgagtcaggt cgagcagttt ttgaccaagt taaaattgat ttacaatcaa
     8881 actagaaaag taagatagga gcattcatgc agtattcaga aattatgatt cgctacggag
     8941 agttgtcaac caagggtaaa aaccgtatgc gtttcatcaa taaacttcgt aataatattt
     9001 cggacgtttt gtctatctat acccaagtta aggtaacagc agatcgcgac cgtgcccacg
     9061 cttacctcaa tggagctgat tacacagcag ttgcagaatc tctcaaacaa gtttttggaa
     9121 ttcaaaactt ttctcctgtt tataaggttg aaaaatctgt agaagttttg aagtcttctg
     9181 tccaagagat tatgcgggac atctacaagg aaggtatgac ctttaagatt tctagcaagc
     9241 gtagcgacca caactttgaa cttgatagtc gtgaactcaa ccaaacactt ggaggggctg
     9301 tattcgaagc cattccaaat gtgcaagttc aaatgaaaag tcctgacatc aatcttcagg
     9361 tggagattcg tgaagaagca gcctatcttt cttatgaaac cattcgtggg gctggtggtt
     9421 tgccagttgg aacttcaggt aaagggatgc tcatgttgtc aggagggatt gactcacctg
     9481 tagcaggtta tcttgctctt aagcgtgggg tggatatcga ggcagttcac tttgctagtc
     9541 caccatatac tagtcctggt gccctcaaga aagcgcagga cttgacccgt aaattgacca
     9601 agtttggcgg aaatatccag tttatagagg tgcctttcac agagattcaa gaggaaatca
     9661 aagccaaagc gccagaagct tatttgatga ctctaactcg tcgctttatg atgcggatta
     9721 ctgaccgtat tcgtgaggta cgaaatggtt tggttatcat caatggggaa agtctaggtc
     9781 aagtagccag ccaaaccctt gaaagtatga aggctatcaa tgctgttacc aacactccca
     9841 tcattcgtcc tgtggttacc atggacaagt tggaaatcat tgacatcgcc caggaaatcg
     9901 atacctttga catttcaatc caaccgtttg aagactgttg taccattttt gcaccagatc
     9961 gtccaaaaac aaatcctaaa attaagaatg cggagcagta cgaagcgcgt atggatgttg
    10021 aaggcttggt tgagcgagca gtggctggaa tcatgattac tgaaatcaca cctcaagccg
    10081 aaaaagatga agttgatgac ttgattgata atctactcta attcagaaaa tccaaaagaa
    10141 tagcgaaaat cagtaaaaaa agttagtttt ttctctaaaa acaggtaaaa aactaacttt
    10201 ttttattttt atgatataat gatataaaat tttgaatata gagagttttc tgacaatgaa
    10261 tcaatcctac ttttatctaa aaatgaaaga acacaaactc aaggttcctt atacaggtaa
    10321 ggagcgccgt gtacgtattc ttcttcctaa agattatgag aaagatacag accgttccta
    10381 tcctgttgta tactttcatg acgggcaaaa tgtttttaat agcaaagagt ctttcattgg
    10441 acattcatgg aagattatcc cagctatcaa acgaaatccg gatatcagtc gcatgattgt
    10501 cgttgctatt gacaatgatg gtatggggcg gatgaatgag tatgcggctt ggaagttcca
    10561 agaatctcct atcccagggc agcagtttgg tggtaagggt gtggagtatg ctgagtttgt
    10621 catggaggtg gtcaagcctt ttatcgatga gacctatcgt acaaaagcag actgccagca
    10681 tacggctatg attggttcct cactaggagg caatattacc cagtttatcg gtttggaata
    10741 ccaagaccaa attggttgct tgggcgtttt ttcatctgca aactggctcc accaagaagc
    10801 ctttaaccgc tatatcgagt gccagaaact atcgcctgac cagcgcatct tcatctatgt
    10861 aggaacagaa gaagcagatg atacagacaa gaccttgatg gatggcaata tcaaacaagc
    10921 ctatatcgac tcgtcgcttt gctattacca tgatttgata gcagggggag tacatctgga
    10981 taatcttgtg ccatccatag tgaaatccct tggtcagaaa atctaccaga ttgtctgaga
    11041 ttttttgcag aaaaatggta agttaagaaa ggaaaaaacg aaatgcatat tgaacatctt
    11101 agccactgga gtggtcatcc taaccgtgaa atgtacctta accgttatgg acatggtggg
    11161 attccagttg tggtctttgc ttcatcaggt ggtagtcaca acgaatacta tgattttggc
    11221 atgattgatg cctgtgcttc ctttatcgag gaaggccgtg tccagttctt taccctatct
    11281 agtgtggata gtgagagctg gttggctact tggaaaaatg ctcatgacca agcggaaatg
    11341 caccgtgcct acgaacgtta tgtgattgag gaggccattc ttttatcaag cacaagacag
    11401 gttggtttga tggcatgatg acgacaggtt gctctatggg agcctatcat gcacttaatt
    11461 tcttcctcca gcatccagat gtctttacca aagtgattgc tctcagtggt gtttacgacg
    11521 cacgtttctt tgtcggtgat tactacaacg atgatgctat ttaccaaaac tcgccagtag
    11581 attatatttg gaaccaaaac gacggctggt ttattgaccg ttaccgtcag gcagagattg
    11641 tgctgtgtac ggggcttgga gcctgggaac aagatggttt accatccttt tacaagctca
    11701 aagaagcctt tgaccagaaa caaattccag cctggtttgc tgaatgggga catgatgtcg
    11761 cccacgactg ggaatggtgg cgtaaacaaa tgccttattt cctcggtaat ctctatttat
    11821 aaaaggagtt acctatgaat taccttgtta tttctcccta ctatccacaa aactttcaac
    11881 agtttaccat cgaactagct aataaaggca tcacagtctt gggaattggt caagagtctt
    11941 acgagcaatt ggatgagccc ttgcgcaata gcttgaccga gtattttcgt gttgataatc
    12001 ttgagaacat agatgaagtc aaacgtgcag ttgcttttct cttttataaa catggtccaa
    12061 ttgaccgcat cgagtctcac aatgaatact ggcttgagct agacgcaaca ctcagagaac
    12121 aattcaatgt ttttggtgcc aaaccagagg atctcaaaaa gacgaaatat aagtctgaaa
    12181 tgaagaaact tttcaaaaaa gcaggtgttc ctgtggtacc tggagctgtt atcaagacgg
    12241 aagcagatgt tgatcaagca gtgaaagaaa tcggtcttcc aatgattgcc aaacctgata
    12301 atggagtggg agcagccgca acctttaaac ttgagacaga agacgatatc aatcacttca
    12361 agcaagaatg ggaccattca accctttatt tctttgaaaa atttgtcact tccagcgaaa
    12421 tctgtacctt tgacgggctc gtggacaagg atggaaagat tgtcttctca acaacctttg
    12481 actacgccta tacaccgctt gacctcatga tttataagat ggacaattct tattatgtgc
    12541 tcaaggatat ggatcctaaa ctgcgcaagt atggggaagc aattgtcaaa gaatttggta
    12601 tgaaagaacg gtttttccat attgagttct tccgtgaggg ggacgattat attaccatcg
    12661 agtacaataa ccgccctgca ggtggtttta ccattgatgt ttataacttt gctcattcct
    12721 tggaccttta tcgtggctat gcagctattg tcgcaggaga ggagttcccg gcgtcagact
    12781 ttgaaactca gtattgtttg gctacttctc gccgtgcaaa tgctcactat gtttattcag
    12841 aagaggattt gcttgccaaa tatagccagc agttcaaggt taaaaaagtc atgccagctg
    12901 ccttcgcgga acttcaagga gattacctgt atatgctgac aactccgagt cgacaagaaa
    12961 tggatcagat gattgctgat ttcggacaac gtcaagaata agaactatcg gattaaggaa
    13021 attcactccc ttaatccttt tgttttgtct ggtaaaaaat aagagcatcc taacaaggta
    13081 gctatcataa aacttgttcg ataactattt gaagcaggat taggtggcta gaaattgaat
    13141 tttaacattt caattgagtc atagtattgt gttttcgtat ccttaaatca gctaaaagga
    13201 tccatgacga cacctatacg atatagtttt caggatacca aacaagtcta ttaatactca
    13261 atgaaaatca aagagcaaac taggaagcta gccgcaggtt gctcaaaaca ctgttttgag
    13321 gttgtggata gaactgacag agtcagtatc atatacctac ggcaaggtga agctgacgtg
    13381 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
    13441 aagctttgtt tgaaatctga taaaataatg atattactaa gttctaaaaa ctaaagaaaa
    13501 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
    13561 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
    13621 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
    13681 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
    13741 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
    13801 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
    13861 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
    13921 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
    13981 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
    14041 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
    14101 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
    14161 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
    14221 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
    14281 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
    14341 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
    14401 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
    14461 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
    14521 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
    14581 atgctgtaat ctcaatgcct agtggtgttt tcaagcctta tgctggagtt tcaactgcca
    14641 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
    14701 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
    14761 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
    14821 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
    14881 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
    14941 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
    15001 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
    15061 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
    15121 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
    15181 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
    15241 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
    15301 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
    15361 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
    15421 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaaaggctta
    15481 ttactaaaag aaaattgcag ttagatgaac taaacttgct cgtcaaatcc cgatttaacg
    15541 agatgtttgg ggaaaataaa atatttgaaa gcattgataa cttatttgat attatagatg
    15601 gtgatagggg caaaaattat cctaaatcag atgagttgtt tagtgaggag tactgtttat
    15661 ttttaaatac aaagaatgtt actaaaaacg gattttcatt cgatacaaag caatttatca
    15721 ctaaaacaaa ggataaatta cttcgaaaag gcaaacttga gcgttatgat atagtcttga
    15781 caacaagagg tactgttgga aatgtagcgt actacgatga attaataaaa tataaacatt
    15841 tacgtataaa ttcaggtatg gtaatattac gtcccaagac accaaatcta aatcagaaat
    15901 ttattatcca tgttttaagg aataataatt atagtcgagt gatatcagga agtgctcagc
    15961 ctcagttacc aattacaaaa ttaaaaaaaa tacttctccc cctcccccca ctagccctcc
    16021 aaaatgagtt cgcagacttt gtagtccagg tcgacaaatc acaatttgct tgtgagatag
    16081 ctataaaagt gtggagaaat agcttgaaat ttagtataat atagctaaac tatttattta
    16141 aagtgagaaa aaaatgggaa attttagctt tcttttaaaa aatgacgaat atgaatcttt
    16201 ttcaaaacct tgcattgaag ctgagaatat gattgctaca tcaactgtgg ctactgcctt
    16261 tatggcgcgt cgtgctttag agcaggctgt ccattggata tatagtcacg attcatattt
    16321 agaagctccc tatcgtgcta ctctatcttc tttagtatgg gatgatgatt ttagggatat
    16381 cgtagattct gaactccaca agcagatagt tctgttgatt cggtggggaa accatgctgc
    16441 tcatggtggt gaaattaagg aacgagaagc gattttagct ttgcatcatt tgtatcagtt
    16501 tgttaatttt atcgattatt gttacagcaa tgagtttgtg gagcgttatt ttgatgagaa
    16561 gtgcttacca ctttcagcaa acatcaaata ccgagaaact ccacaatcta tgataaagtt
    16621 acaagacagt ttaccagaac tgcctgattt tcatgaacag atggctgctc agtccgtaga
    16681 agttcaagag acttatactg aaaaacgtga gactgcagcg caacggcaag atgtgccttt
    16741 ccatattgat caattatctg aggcagagac aagaaagctc tttattgata tcgatctccg
    16801 tttagcagga tggatatttg aagaaaactg tcgtgttgag atagccgttg atggtctcaa
    16861 gcacggttca ggaattggtt actgtgacta tgtactttat ggtaaaaatg ggaaaatttt
    16921 agcgattgtg gaggctaaaa aagcctctgt caatccagaa gtaggggaag tacaggtcaa
    16981 agaatatgct gaagctttgg agaaacatat cggctatcag ccaatttgct ttattacaaa
    17041 tgggttgaag cactatatac ttgatggtcc gaaccgccgc cagattgcag gcttttactc
    17101 tcaagaagaa ttgcaattag tgatggatag acgtcatctt caaaaaccgc ttgaggatat
    17161 ttctagtaaa attagggacg atatttccgg gcgtcactac caaaaacatg ccattgcaag
    17221 cgtttgtgaa gctttctctg atcatcgtag acaggcactt ttggttatgg caactggggc
    17281 ggggaaaact cgtacagcag tttctctagt tgatatctta tcacgtcata actgggtaaa
    17341 aaacgttctc ttcttagccg atagaacttc cttggttaag caagcctatg attcgtttag
    17401 aaaattactc ccagatcttt ccgtttgtaa cttcttagaa gataaagaag gagctcaatc
    17461 aagtcgtatg gtcttttcaa cttatccgac catgattgga gcgattagtg gtcaagaaga
    17521 agtaaatcaa cgccctttta ctgttgggca ttttgacctt atcataattg acgaatctca
    17581 ccgttctatt tatcagaaat acaagtccat ttttgattat tttgatgcaa ggattgtagg
    17641 cttaacagct actccgcgtc aagatttaga taaaaacacc tatggattct ttaatttgga
    17701 gaatggggtt ccaacatatg catatgattt ggaagaggct gttaaagacg gatatttagt
    17761 agcctatcat tctatcgaaa ccaaactgaa gctacctacg gatggtctac attatgatga
    17821 tttgtccgaa gaagaaaagg aacattttga tagcaaattt gaagacgata gctgtgaaaa
    17881 agatattgat gggagtgtgt ttaattcctt tattttcaat aaaagtacag tagaaattgt
    17941 tttaaatgaa ctcatgacaa gaggaattca gacagcctcg ggtgatgaaa ttggtaaaac
    18001 tattattttt gctaaaaatc atgaccatgc ggaatatatc agaggtattt ttaacaaccg
    18061 ctatcctgaa aaagggagcg actatgctca ggtgattgat tatagtatta agcattatca
    18121 gaccttgatt gatgatttta aaattaagga gaagtatcct caaattgcga tttctgtcga
    18181 tatgttagat acaggtattg atgtaccaga ggttgttaat ttagtcttct tcaagaaagt
    18241 acgctctaaa actaagtttt ggcagatgat tggtcgagga acccgtctat gtaaagattt
    18301 atttggacct gagcaggata agaaaaactt cttggtattt gattatgggg acaattttga
    18361 ttattttcgt gcagatccaa gagatggaga gggtcgtcac attgtttcgc tgactcagcg
    18421 tttatttaat atcaaagtgg acttgattcg agaacttcag ggactccaat accaagaaga
    18481 tcagtttgcg agagcatacc gtcagcagct tgtctcggaa cttcaaggtc gtatagagag
    18541 cttaaatgag ttggacttca gggttcgtat ggttttagat acagtttata gctataggaa
    18601 attggaaagt tggcagaatc taactgctgt tacaagtgaa accattcaaa aaaatctctc
    18661 tccgctttta tttgatgaag ataaagaaga tgagatggcg aggagatttg atttgtggtt
    18721 gcttcatatt cagttggggc aactgacagc taaatcttcc actgttcata tttcccaagt
    18781 gatgaagacg gctagagctc tttctgctat tggcaatatc ccgcaggttt ttgagcaggc
    18841 tgaaattatc agggaagtac aggagcctga attttggaaa gaagttaact tgtctgattt
    18901 ggaaaaaatt cgtcttgcta ttcgagattt attacagttt ttggataaaa cagaccgtaa
    18961 accctactat gttaactttg aagatcgtat actctccact gttcacgaga ccacagcatt
    19021 tttgcaggtc aacgatcttc ggtcttacaa tgaaaaagtt gacatctgat gatatgcttg
    19081 cacttgaaaa attgctttgg gaaaaattag gtagtaaagc agactaccaa agtcattatg
    19141 aaaataaggc aattccgaga ttggttcgtg agattattgg cttagataga gagtctgcca
    19201 atcgtatttt ttctaaattt ttgtcggatg agaatcttaa tgccaggcag atttcatttg
    19261 taaaattgat tgtagactac attgtagaaa atggtttttt agagacgaaa gtgttaacgc
    19321 aagagccgtt taaatcttat ggttctgttc aactactctt ccaacaccaa ctaccagtac
    19381 ttcgtaatat tgttcaaatc attgaactta tcaataatcg agctggagaa gcggcttaaa
    19441 ttctaaagtg attgccatgc tgagactcat ttaaaattaa aaagagtaga aatttatgat
    19501 atatatgaga agttttatta ggaagaatgt catcgttttt ctagaataca gtatcagttg
    19561 ttaagtggct gataaatttc aaagtagata cttgtaccac gatgtttgtt gatcgagtta
    19621 ttaacaaaag agctactttg attttaaaga aatagaaaac aaaaagccga gcaagaattc
    19681 aattgcagga gaaaatgaaa taatactcaa tgaaaatcaa agagcaaact aggaaactag
    19741 ctgcaggctg ctcaaaacac tgttttgagg ttgtagataa gactgacgaa gtcagctcaa
    19801 aacatggttt tgaggttgta gatgaaactg acgaagtcag taaccatacc tacggcaagg
    19861 cgacgttgac gcggattgaa gagattttcg aagagtataa atcttcctag gataaagcaa
    19921 aacgcatagt atcaagggtt ttcaacactt gatactatgc gttttctgat gttaaagact
    19981 ttctaccagt ttttttaagc ataattgtta gttgtagtca tttattattc ttcaaagaaa
    20041 aatggtgggg cgaatttttt cagttcttca aagcactttt gagcagtatc tgcatcttca
    20101 cagatgataa gacagacatc attaccacaa agggtagcga tagcgtcagg gaagctcaaa
    20161 gtatcaatga tagaaccaaa ggattgagcc agtccaggaa gggtttttag taggacttgg
    20221 tgttgaactg ggcgcatcca gacaagggcg tcttccatgt agagttcgag acgtttttcc
    20281 cattttgaga tggaaccatt gttaagaaca taataagcgc tatcttcttc gcggactttt
    20341 gataggttca tatttttgat gtcgcgtgag agggttgcct gggttacttg aatgtcgttc
    20401 ttagcaagaa gggcttgcaa ctcagcctgt gtatgaatct tgttttttgt gataagagcg
    20461 cgtataagtt ggtggcggtg ttctgattta ttcataataa tgtaactcct tttagcaagg
    20521 taaggtaagc atggactgag cgaggtcgac agtcaagtgg tagtctgtat tgtcacggat
    20581 ggtgatttca aagtcagtag tatagaggac taaacggaga gtgtctcctt cttttagctt
    20641 gtaaatagtt ggctgcagtt caaattgaac gtccatccat tcatctgcag taatatcctc
    20701 tactaacagt aaatcatttc tattttgtaa attaaggtaa ccttttgtca cgactcgttg
    20761 tgcctctggt ctaaatggca attcacagag attttccaac atgtgatagc gaccgttgtc
    20821 aatggttcta gcacttaaaa tagctggata aggttgtagg tatttctttt gcccaaattc
    20881 tagcagttgg gcagataaga gccccttgtt tgtactggat ttgatacgaa gattgagctg
    20941 agcgcgacca tttaggtgga gatctttagt cacaggaagg ttaatagtaa tctgattggc
    21001 tttcccttga tagagctctg tattgaaggt ttggtatgtc ttaccatagc gctcaaaatc
    21061 cttatctggg tactggtttt gaatagcttg ctcttcttga ccaagtgaga aggtttcaca
    21121 gttttcttgc ccaccgaagt tatcaagtga taaccaagtc tgtggagctg tattgtcctg
    21181 ccagataaca gtaggaagtt gaaagtctgt ttcctgtcct agtaatttct tggtcaataa
    21241 ggcatttatg gactcacgga agtcaattga ttgccaattg ttcatgtaaa catgagcacc
    21301 attatggaaa aagagatgct tgtgtatatg agtaggaaga gcatggaaca tctggtaaac
    21361 atgaagtggt ttgacattcc aatcctgaga accatgagta aagacaacct ctgcctttac
    21421 tttatgggca ttgagcagat aattgcggtc atgccaaaac tgattgtagt ccccagtttt
    21481 tcgatctagc tgagctttca ctttttctaa gtcagcttgg tgagcttcat tgccacggat
    21541 atagtcgcca gctaagagat tacgagaata ggttaactca gcaagggagt caaagtcctc
    21601 acctggataa ccacctgggc tagtcaccag accgttttca cggtagtagt tgtaccatga
    21661 tgaaattcct gcctcggcaa tgataacttc taaaccatcg actcctgtag tcgcaagacc
    21721 attggacatg gtacctagat aggaaagtcc tgttgtagca acttttccgt ttgaccaatc
    21781 agccttgact tgacgctggc gcgtgtgatc agtaaaggca cggcaacggc cgttaagcca
    21841 atcgatgaca tttttataag cctcgatttg ctggtagtct ccattagtca tgaaacctgt
    21901 cgagtctttg gtaccaacac ctgagacata gagattggca aagcctctcg gaaggaagta
    21961 gtcgtttagt gtatagctag agttgatgtg agttagcttt tcctcagcct ctgctataag
    22021 ctcagcttta ccttggggtt ggacgagatt tagttgaggt ttctctagct caatcttgtg
    22081 aggaagctta acctcaagct cgccctccat cttgtagaga gccttgtcac tagccttgtc
    22141 attggttccc tgatgataag ggctggctgt catgatggca gggatttttc catcaaaacg
    22201 agggcgaata atgctaacct ttactaggtc tggtagccct ttttggtcag tatcgacacg
    22261 agactcaacg taaacgactt cacgaatgac atcctggcta gaaaaagtag ccaaactctt
    22321 gccgttaaag tagtggtagt cattatcctc cggaataaga ccatcactaa caagttggtc
    22381 gataagagta tttccttttt tggtgcgagt atttagtaac tgatagagat tttcaatcaa
    22441 gtcaccatat ataatgggaa atccagtttc tttacgaaaa acgtcactat cttcgaagtc
    22501 aaccaaataa gaaaagccta aaagttgaaa agcaacagta taaaaaatat ctgctgtcag
    22561 ttcatcttct gattgaaaaa atgtcagcag gtctgttttt ttatcagctg ctaggataga
    22621 aagtgggtag ttggtgtctt gataagtgaa aaagaaacga cgtaaaaagg tttcaagtga
    22681 gtctttgtga ttggctgtat tttgtaaatc aaagccacat ttttttagtt cagataagac
    22741 attttctttt ggaaaattga tataactata ttgattaaaa cgcatagaac ctccatatag
    22801 aatgacagtt aaggttatta tatcaaaaaa aaagcagaaa gggaattgtt aacttcaaaa
    22861 ggaaataatc caataaaaat gaat
//
LOCUS       DAVLRT010000026        22421 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLRT010000026 DAVLRT010000000
VERSION     DAVLRT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.026"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            110..706
                     /locus_tag="VXM85_000983"
                     /pseudo
     CDS             110..706
                     /locus_tag="VXM85_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            782..1231
                     /locus_tag="VXM85_000984"
     CDS             782..1231
                     /locus_tag="VXM85_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1345580.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            1244..2335
                     /locus_tag="VXM85_000985"
     CDS             1244..2335
                     /locus_tag="VXM85_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345581.1"
                     /translation="MSEIEIINAKKIYHDVSVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNLSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGCTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            2325..3970
                     /locus_tag="VXM85_000986"
                     /pseudo
     CDS             2325..3970
                     /locus_tag="VXM85_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4073..4765)
                     /locus_tag="VXM85_000987"
     CDS             complement(4073..4765)
                     /locus_tag="VXM85_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1345582.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(5009..6346)
                     /locus_tag="VXM85_000988"
     CDS             complement(5009..6346)
                     /locus_tag="VXM85_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1345583.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(6356..6622)
                     /locus_tag="VXM85_000989"
     CDS             complement(6356..6622)
                     /locus_tag="VXM85_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1345584.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(6887..7273)
                     /gene="rplQ"
                     /locus_tag="VXM85_000990"
     CDS             complement(6887..7273)
                     /gene="rplQ"
                     /locus_tag="VXM85_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1345585.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(7285..8220)
                     /locus_tag="VXM85_000991"
     CDS             complement(7285..8220)
                     /locus_tag="VXM85_000991"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1345586.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(8263..8646)
                     /gene="rpsK"
                     /locus_tag="VXM85_000992"
     CDS             complement(8263..8646)
                     /gene="rpsK"
                     /locus_tag="VXM85_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1345587.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(8664..9029)
                     /gene="rpsM"
                     /locus_tag="VXM85_000993"
     CDS             complement(8664..9029)
                     /gene="rpsM"
                     /locus_tag="VXM85_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1345588.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(9047..9163)
                     /gene="rpmJ"
                     /locus_tag="VXM85_000994"
     CDS             complement(9047..9163)
                     /gene="rpmJ"
                     /locus_tag="VXM85_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1345589.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(9188..9406)
                     /gene="infA"
                     /locus_tag="VXM85_000995"
     CDS             complement(9188..9406)
                     /gene="infA"
                     /locus_tag="VXM85_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1345590.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(9523..10161)
                     /locus_tag="VXM85_000996"
     CDS             complement(9523..10161)
                     /locus_tag="VXM85_000996"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1345591.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(10312..11622)
                     /gene="secY"
                     /locus_tag="VXM85_000997"
     CDS             complement(10312..11622)
                     /gene="secY"
                     /locus_tag="VXM85_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1345592.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(11635..12075)
                     /gene="rplO"
                     /locus_tag="VXM85_000998"
     CDS             complement(11635..12075)
                     /gene="rplO"
                     /locus_tag="VXM85_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1345593.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(12220..12444)
                     /gene="rpmD"
                     /locus_tag="VXM85_000999"
     CDS             complement(12220..12444)
                     /gene="rpmD"
                     /locus_tag="VXM85_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1345594.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(12416..12910)
                     /gene="rpsE"
                     /locus_tag="VXM85_001000"
     CDS             complement(12416..12910)
                     /gene="rpsE"
                     /locus_tag="VXM85_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1345595.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(12928..13284)
                     /gene="rplR"
                     /locus_tag="VXM85_001001"
     CDS             complement(12928..13284)
                     /gene="rplR"
                     /locus_tag="VXM85_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1345596.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(13368..13904)
                     /gene="rplF"
                     /locus_tag="VXM85_001002"
     CDS             complement(13368..13904)
                     /gene="rplF"
                     /locus_tag="VXM85_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1345597.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(14096..14494)
                     /gene="rpsH"
                     /locus_tag="VXM85_001003"
     CDS             complement(14096..14494)
                     /gene="rpsH"
                     /locus_tag="VXM85_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1345598.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(14779..15048)
                     /gene="rpsN"
                     /locus_tag="VXM85_001004"
     CDS             complement(14779..15048)
                     /gene="rpsN"
                     /locus_tag="VXM85_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1345599.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(15066..15608)
                     /gene="rplE"
                     /locus_tag="VXM85_001005"
     CDS             complement(15066..15608)
                     /gene="rplE"
                     /locus_tag="VXM85_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1345600.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(15632..15937)
                     /gene="rplX"
                     /locus_tag="VXM85_001006"
     CDS             complement(15632..15937)
                     /gene="rplX"
                     /locus_tag="VXM85_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1345601.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(16015..16383)
                     /gene="rplN"
                     /locus_tag="VXM85_001007"
     CDS             complement(16015..16383)
                     /gene="rplN"
                     /locus_tag="VXM85_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1345602.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(16409..16669)
                     /gene="rpsQ"
                     /locus_tag="VXM85_001008"
     CDS             complement(16409..16669)
                     /gene="rpsQ"
                     /locus_tag="VXM85_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1345603.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(16694..16900)
                     /locus_tag="VXM85_001009"
     CDS             complement(16694..16900)
                     /locus_tag="VXM85_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1345604.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(16910..17323)
                     /gene="rplP"
                     /locus_tag="VXM85_001010"
     CDS             complement(16910..17323)
                     /gene="rplP"
                     /locus_tag="VXM85_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1345605.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(17327..17980)
                     /gene="rpsC"
                     /locus_tag="VXM85_001011"
     CDS             complement(17327..17980)
                     /gene="rpsC"
                     /locus_tag="VXM85_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1345606.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(17993..18337)
                     /gene="rplV"
                     /locus_tag="VXM85_001012"
     CDS             complement(17993..18337)
                     /gene="rplV"
                     /locus_tag="VXM85_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1345607.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(18349..18630)
                     /gene="rpsS"
                     /locus_tag="VXM85_001013"
     CDS             complement(18349..18630)
                     /gene="rpsS"
                     /locus_tag="VXM85_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1345608.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(18734..19567)
                     /gene="rplB"
                     /locus_tag="VXM85_001014"
     CDS             complement(18734..19567)
                     /gene="rplB"
                     /locus_tag="VXM85_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1345609.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(19585..19881)
                     /locus_tag="VXM85_001015"
     CDS             complement(19585..19881)
                     /locus_tag="VXM85_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1345610.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(19881..20504)
                     /gene="rplD"
                     /locus_tag="VXM85_001016"
     CDS             complement(19881..20504)
                     /gene="rplD"
                     /locus_tag="VXM85_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1345611.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKLVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(20529..21155)
                     /gene="rplC"
                     /locus_tag="VXM85_001017"
     CDS             complement(20529..21155)
                     /gene="rplC"
                     /locus_tag="VXM85_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1345612.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(21372..21680)
                     /gene="rpsJ"
                     /locus_tag="VXM85_001018"
     CDS             complement(21372..21680)
                     /gene="rpsJ"
                     /locus_tag="VXM85_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1345613.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(21946..>22421)
                     /locus_tag="VXM85_001019"
     CDS             complement(21946..>22421)
                     /locus_tag="VXM85_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1345614.1"
                     /translation="NLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDIL
                     ALQAGMDVLTIEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELK
                     RRLARDTTVRNRYASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
BASE COUNT         6400 a         4896 c         4144 g         6981 t
ORIGIN      
        1 caaagtgtac tgttatagat tcaatacact atagaataga attgtaggtt ttaaaaacgc
       61 ttacattgtt ttaaaataaa cttaaataac acaacggaga tatcccatca tgaaaaagaa
      121 atggatgtat tatgctgctt gttcttctaa tgaatctgcc gatgacagtt catctgataa
      181 aggagacggt ggttcgctag tcgtttattg accaaactca gaggacttaa ttggagcaac
      241 tattcctgcc tttgaagaaa aatatggtat caaagtagaa ctgattcaag ctggtactgg
      301 agaacttttc aaaaaactag agtcagaaaa agaagttcct gtagctgatg ttatctttgg
      361 tggttcttat acacaatatg ctacccacgg agaactcttt gaaaactata cttcaaaaga
      421 aaatgataat gttatcaaag aatatcaaaa cacaactggc ttctctactc cttatacact
      481 agatggtagt gttttaatcg tccaccctga tttaactaaa ggcatgaaca tcgaaggata
      541 tagcgacctt ctcaaacctg aactaaaagg aaaaatcgca actgctgacc cagcaaactc
      601 ttctagcgcc tttgctcaat taacaaatat gctacaagct caaggtggtt acaaagatga
      661 taaggcttgg tcttatgtaa aagatctttt cacacttatt gatggtatag taaaatgaaa
      721 taagaacagg acaaattaat caggacagtc aaatcgattt ctaacaatgt tttagaagta
      781 gatgtgtact attctagttt caatatacta taaaatcggt ttaagttcat ctggtgtcta
      841 taaagcagtc gctgatggag aaatgactgt tggtctctct tatgaagatc cagcagttaa
      901 actcttaaat gacggagcta acattaaggt agtctatcca aaagaaggaa ccgtcttcct
      961 acctgctagt gctgctatcg ttaaaaaatc taaaaatatg gaaaatgcca agaaatttat
     1021 cgattttatt atctctcaag aagtacaaga tacacttggt acaaccacta ctaaccgtcc
     1081 tgttcgtaaa aatgctaaaa caagcgaaaa catgaaacca attgacaaaa tcaaaacact
     1141 cactgaagat tatgattatg tcatcaagaa taaatcagat atcgttaaga aatacaacga
     1201 agtctttaca gatatccaat ctaaacagta aaagaggttc actatgagtg agatcgaaat
     1261 tattaacgcc aaaaaaatct accacgatgt ctctgttatt gagaatttga acattacaat
     1321 tccaaaagga agtctcttta cccttcttgg agcttcagga tgtgggaaaa cgacccttct
     1381 tcgtatgatt gcaggtttca acagtatcaa agatggagaa ttttacttcg atgatacaaa
     1441 aatcaataat atggaaccca gcaaacgcaa tatcgggatg gttttccaaa actacgctat
     1501 tttcccacat ttgactgtcc gagacaacgt tgcttttggt cttatgcaaa agaaggttcc
     1561 aaaagaagaa ttgattcaac agaccaacaa gtatcttgaa ctcatgcaaa ttgctcaata
     1621 tgcggatcga aagcccgata aactcagtgg tggacaacaa caacgtgtca ccttggcatg
     1681 cgccttagcg gttaatctaa gtgttctcct catggacgag ccacttagta atctggaggc
     1741 caaacttcgc ttggatatgc gtcaagccat ccgagaaatc caacacgaag tgggaattac
     1801 aactgtttat gtaacccacg accaagaaga agccatggct atttcagacc aaattgctgt
     1861 tatgaaagat ggggtgatcc aacaaatcgg ccgaccaaaa gaactctatc ataaaccagc
     1921 taatgagttt gtggcaacct ttatcggatg cacaaatatt atccctgcca atcttgaaaa
     1981 acggagcgac ggcgcttata tcgtcttttc agatggctat gcccttcgaa tgctagctct
     2041 tgatcaggtt gaggagcaag ctattcatgt aagcattcgt cccgaagagt ttatcaaaga
     2101 tgaatctgga gatattgaag gaactattag cgatagcgtc tatcttggac taaatacgga
     2161 gtatttcatt gagacaggtt ttgcctcaaa aattcaagtt agtgaagaat caacttttga
     2221 agaagatcta caaaaaggca atcgtattcg tctacgaatc aatacgcaaa aattaaacat
     2281 cttttctgca gatggttccc aaaacctgat aaaaggagtc aaccatggaa cgtaaaaaac
     2341 taaatatttg gacagcctcc tctttcttca tctttcttac ctatcttgtc tttctcgttt
     2401 atcctatcgt taccgtgctc aagtaagcac ttatacatga aggacaattc tcactagcta
     2461 attttgtcac tttctttagt aaagtctact actctgagac actagtcaac agtttcaagg
     2521 tttccattac cgctactgtc acttccttag ttgtaggaac cctattagct tatctcttct
     2581 ctatgtatga cttcaagggg gagaaatttc tacaaatatt gattatcatt gcttccatgt
     2641 cagctccttt cgtaggagcc tactcctggg ttctcttgct aggacgaaat ggggtcatca
     2701 ctaaattttt gacaaatgcc cttcatcttc cagctatcga tatttatgga ttcaaaggaa
     2761 ttgtacttgt ctttacactg caactattcc cactggtatt tctatatgtt gctggaacaa
     2821 tgaacagtat tgacaattct ctacttgaag cagctgaaag catggggtcc ttcggattta
     2881 agcgtatcgt aacggttgtt ttacctctcc tagttccaac cttactagca gttccttgct
     2941 tgtatttatg agagcattct cagactttgg aacgcctatg ttgattggcg aaggatatcg
     3001 gactttccct gtcctgattt atacccaatt tattagcgag gttggaggaa attctgcttt
     3061 tgcaattatg gcgattatca ttgccttggc gattttcctt atccaaaaac acattgcaaa
     3121 ccgctacagt ttcagcatga atctgctcca tccaattgag cctaaaaaaa ctacaaaagg
     3181 aaaaatagct gccatttatg caacagtcta cggaattatc tttatctctg ttttacctca
     3241 aatctactta atttatacct ctttcctaaa aacatcaggt atggtatctg ttaaaggtta
     3301 ttctccaaac agttacaagg tagctttcca tcgtatggga tctgctattt tcaataccat
     3361 tcgtatccct ttgattgcct tagttctagt tgttctattt gcgacattta tctcctacct
     3421 agccgttaga aaacggaatt tgtttacaaa cttaattgac agcctcagta tggtacctta
     3481 tattgtacca ggaaccgttc tagggattgc cttcatttct tccttcaata ctggtctatt
     3541 tggaagtgga tttcttatga ttacagggac tgctttcatc ttgattatgt ctctatctgt
     3601 cagaagatta ccttatacta ttcgctcatc tgttgctagc ttacaacaaa tagcaccaag
     3661 tattgaagaa gctgctgaaa gcttaggaag tagtcgtctc aatacctttg ctaagattac
     3721 aactccaatg atgctatctg gtatcatttc tggagccatc ttatcttggg tcacaatgat
     3781 ttcaaaactc tctacttcta tcctcctcta caatgtcaaa acaagaacaa tgactgtagc
     3841 tatttataca gaggttctca gaggaaatta cggtgtagcc gcagccttgt caactatcct
     3901 gactgttcta acagtaggtt ccttgctctt gtttatgaaa atctctaaaa gcaatagcat
     3961 tacactttag ttttccacat caaaaacagc cgaaaggagt accctcggct gttttttctt
     4021 atcttgatat tcttatcaaa tccctagctc atggcaagca agtctagact gattaaatag
     4081 agccttcttc catcttctca cgtcctagct ctcggtaact acggtcaccg acaacttcaa
     4141 ccctaaactg accgtcctca tattcaagaa tcgtcacgct accattatct agaccatgcg
     4201 gatgcatgcc attaatcaga taaacaatgg ttccaatagt cattccatgg ctgacaacaa
     4261 gggcgttacc tccaccttga tcttccattt cttttgcaat catttcaaag ccttccttga
     4321 ttcggccact gagtttttcc cagccttcag cccaaccagc tgtatcgacc tctaccaagc
     4381 cctcagccag ttcagcataa gacaattggt gaacgtggtc cacattaaag atacgaggaa
     4441 taatgcccat gaaaagatcg ccatcatagg ctccatcaaa actaccgaaa caccattctc
     4501 tgatacgctt gtccatgcga taagggattt tcccctgcaa gccaagttct tcaaggataa
     4561 ttcccatggt ctgaatggta cgaccagaat cactcgaata agcgcgctca aactgtagat
     4621 cagattctcg caaaccgatt cctaactctt gaatccctcg ttcaccttca gcagttaagg
     4681 gagtatcgct ccaaccttgc gcgcgaccaa tcgtgttaaa catggtcttg ccatgacgta
     4741 ccaaatacaa tcgtactttt accattttcc gtcctccgtt tgcttctatt atatcatgaa
     4801 tgataaaata aaagccaccc atacaggcga cttttgaagg agattattat gaaaaagttt
     4861 aggagttcat taaataaaga tattagatga aaatcaaatt caaactaatt cagtgttatc
     4921 tgtttcaaat aatattagga ggtctttatt taatgattat agtatacacg tctaatctta
     4981 aatagaactt aaaatttctc ctattttctt aatttttaaa actatgaatt ggtgctggga
     5041 tttgtccacc gcgagagatg aagtcgacag acgaagcccc attaaccttc ataacaggtg
     5101 cagttcctaa tagaccacca aactcaatca tatcgccttc tcttcctttg ggaatgatac
     5161 gaacagctgt tgttttcatg ttgataacac cgattgctgc ttcatccgca atcatagccg
     5221 caatggtttc agcaggcgta tcttctggga tggcaatcat atccaatcca acagaacaga
     5281 tagccgtcat agcttctagt ttttctaaat taagagagcc attttgcact gcagcaatca
     5341 ttccttcatc ctcagaaaca gggataaagg caccagataa accaccgact tggttgcagg
     5401 ccatcactcc acccttttta acttggtcgt tcaagagggc caaggcagcc gtcgttccat
     5461 gcgtgccaac tgtttctagc cccatttcct caaggacacg tgccacagag tctccaaccg
     5521 caggggttgg tgccaaactc aagtccacaa taccaaactc cacacccagt ctctcactgg
     5581 ccatttgacc aaccaattga ccgatacgag tgattttaaa ggcagttttc ttaactgttt
     5641 cggctactac atcaaagctc tgtccacgaa ctttttccaa agcacgtttc acaacaccag
     5701 gaccagaaac tccgacattg atgataacat ctgcttcccc aacaccatga aaggcacccg
     5761 ccataaatgg attgtcctca acagcattag cgaatacaac caacttggcc actcccatat
     5821 ctgaaagatt tgctgtttcc ttgataattc gtcccatatc tgccacagcc gtcatattaa
     5881 taccagactt ggttgagccg atattgactg acgagcagac cttatccgtc tcagccaaag
     5941 cgcgaggaat ggaattgatg agaatctcat ctcccttttg ataacctttt tgtactaagg
     6001 cagaaaaacc accaataaag tccacaccaa tctctttcgc agccttatca agcgcttttg
     6061 ccagaaccac gtagtccgtc gcatctgtcg ctgccccaat cagagaaata ggtgtcaccg
     6121 atacacgctt attaacgata ggaattccca actcagccgc aatttcatca ccaacagcta
     6181 ctaaattagc cgcctttgtc gtaatttttt gatagatttt ctccgcagca cgattgatat
     6241 ttggatcgat acagtccaaa agagaaatcc ccatggtaat ggttctaata tcgaagtttt
     6301 gctcctcaat catggcgatg gtttcagtaa cttgtctaat atccatgatg acctcctaga
     6361 tattatacat agcttcgaaa atcgctgcac tctgaatatt gatttttaca ttcaaagttt
     6421 gcccaaaagc ttcaaattca ttacgaagat aggtaaaatc ttgcttttca tcactagata
     6481 caacagccat catcgtaaaa tattcatcca atacagtttg agagatatcg tcaatattca
     6541 atcctaattc tgcaatttta ccagaaacac ctgcaacaat tccagattta tctttaccaa
     6601 caacagttat aatcgctttc ataagtaaac tccaattctt cttaaaataa gaaaccttaa
     6661 cattaaacag gattcttttc aaatagtata gcataattct aacgaaaata ctcaaaaacg
     6721 cctgcttacg aagttgttct taagaaaaaa acaatttcgt aaacaagcgt ctactatccc
     6781 aacttatgat gagtgttccc gctaggaccg aaatcctagc ggtagaccag agctagacta
     6841 agagcacaag actccatcat cataacactc aacaaaattg atgattttat actaattcga
     6901 tgatcgccat tggcgctgca tcaccacgac gtggttcagt tttaaggata cgagtgtatc
     6961 caccgttacg ttcagcataa cgaggtgcga tttctgagaa caatttttga agtgctgtag
     7021 tagaagtgta cttatcagtt gcttcatcat agttttcaga tgcgatttca ttacgtacga
     7081 aagcagctgc ttgacgacgt gcatgcaaat caccacgttt acctagagta atcatttttt
     7141 caacagtttt acggatttct ttagcacgag cttcagttgt cacgattgat tcgttgatca
     7201 aaaggtcagt tgtcaaatcg cgaagcattg ctttacgttg tgagctagtg cgtcctagtt
     7261 tacggtaagc catgtattcc tcctttattt atcttttaat ccaagaccca aatcaatgag
     7321 tttgagtttc acttcttcca aactcttgcg tccaagattt cgtactttca tcatctctgc
     7381 ttcagatttt tctgtcaaat catgcacagt attgataccg gcacgtttta aacagttgta
     7441 tgaacgcaca gacaagtcca gttcctcaat cgtacgatct aaaatacggt cgtcagattc
     7501 agtatcagct tctttcatca cttcagttga cttagcaatc tcagtaagat ttgtaaacaa
     7561 atcaagatgt tctgtcaaaa tacgtgctga aagccctaaa gcatcttctg gaataattgt
     7621 tccatttgtc aagatttcaa gggttaattt gtcgaaacca tcattgctac ctacacgagc
     7681 aggttccact tgatagttga cttttgtaac tggtgtataa atagaatcta cagcaagtgt
     7741 tccaactggt gcattatcct ttttattttc atcagcaggt acatatccac gaccactgtt
     7801 aacagtcata gtcgctttta gagaagaacc ttcaccgatt gtaaagagat aatgatctgg
     7861 atttacaatt tcaatatcgc tatctgtcaa aatgtcacca gctgttactt cagcaggacc
     7921 ttcaacatcc agttcgatga ttttttcgtc ttcaacgtac gatttcactg caattccttt
     7981 aatgttcaga atgatttgca tcacgtcttc acgaacacct ggaactgtgt caaactcatg
     8041 taacacacca tcaatgttga tagatgtcac agctgctcct ggtagagaag ctagaagtac
     8101 acgacgaaga gagttaccaa gagttgtacc gtagccacgt tcaagtggtt cgattacaaa
     8161 cttgccataa tctttatttt catcaatttt tgttatattt ggtttttcaa actcgatcat
     8221 ttagttactc cctcttaaac gaaaagcagt gtaatgcgat gattatacac ggcgacgttt
     8281 tggaggacga gcaccattgt gtggcactgg agtcacatca cgaattgctg ttacttcaag
     8341 accagcggca gcaagcgcac gaatagctga ctcacgacca gaacctggac cttttacagt
     8401 aacttcaact gatttaagac cgtgttcttg tgcagattta gcagcagctt cagaagccat
     8461 ttgagcagcg aatggtgtag atttacgaga acctttgaaa ccaagagcac cagctgatga
     8521 ccaagcaatt gcattaccat gcacatcagt aatcataaca atagtgttat taaatgtagc
     8581 gtgaatatga gcaataccag attcgatatt ctttttcaca cgacgtttac gtgttggttt
     8641 agccaagact tttacctcct atattatttt ttcttaccag caatcgcaac agctttacct
     8701 ttacgagtgc gagcgttgtt tttagtattt tgtccacgga caggaagtcc acgacggtga
     8761 cggataccac ggtatgaacc gatttccatc aaacgtttga tgttcaagtt tacttcacga
     8821 cgaaggtcac cttcaacttt gattgcatcc acttcacgac ggatagcatc ttcttgatct
     8881 gatgtaagat cacgtacacg aacatcttct gagattccag cagcagccaa aattttctta
     8941 gatgttgcaa gtccgatacc ataaacataa gtcaatgaga ttactacgcg tttgtcattt
     9001 ggaatatcaa ctccagcaat acgagccatg ttttctcctt tctatcttat ccttgacgtt
     9061 gtttgtgttt tggatttgct gggcaaatta ccataacacg accattacga cgaataactt
     9121 tacagtattc gcaaattggt ttgaccgatg gtcttacttt catttcttat ccctccaagt
     9181 ttttcgatta tttaaagcgg taagtgatac gtccacgtgt caagtcatat ggactcattt
     9241 cgacagtaac acgatctccc gctaaaatac gaatatagtt tttacgaatt ttaccagaaa
     9301 ctgttgctaa aatctgatgt ccattttcaa gttcaaccgt aaacattgca ttcggcattg
     9361 tatcaactac tttgccttca acttcaatca catcgtcttt tgccacgcaa aagcacctcc
     9421 ataaatttcg atttgatgcc tctagacaca gagacaacaa ttataagtca gactatttca
     9481 gtataacatt tgtagcggat ttttgcaagt gtgaaaaacg ctttatttca aatttgtcaa
     9541 tactttttca atatctgaga agacatcatt gatatcttga ttaccttcga tgtcatgaac
     9601 caaacctttg gcacggtagt gagcaatgat tggttctcct tgagcaatat taacatccaa
     9661 acgacgtttt actgtctcag gcttatcatc ttcacgttgg tagtaatctt cttctttata
     9721 gtcaactggt gggttaaaga ccttgtggaa agtttctcca gttacgcggt ggatgatacg
     9781 gccactcaaa cgttccaaga ggctgtcagg gttcacttca atattgataa taccttctag
     9841 ttcaatgcca agttcagcca atgttttgtc caaggcatga gcttgttcaa ttgtacgtgg
     9901 gtaaccatcc aataagaatc ctgtttcttt aatatcatct tgtgaaaggc gttcttttac
     9961 gattccattt gtaacttcgt caggaaccaa ttcacccttg tcaatatatg acttagcaag
    10021 aacacccatt tcagtttgat ttgccattgc agcgcggaac atatcacctg ttgagatatg
    10081 tgcaacatgg aattgttcta cgatttttgc tgcttgagtt cccttacctg caccaggtaa
    10141 gcccataatc aaaagattca tgatttgatc tccttatttt atttttaaat agaagcaaaa
    10201 agtcttttca cccctattta aaaacaaaat agaagagggg agatccaact cttacaaatg
    10261 tcagagttta aacctccact ccctcagtat ttactgattc agtaaatact tttattctgt
    10321 tctgtccatg aaaccaacat acttacgttt caataggtaa ccttccaatt gcttgattcc
    10381 ttcgatacct gtagagataa tgatcaagag acttgttcca ccaaaggcaa caacatcaga
    10441 aagaccaaat acatctttag ctgcaatcgg taaaatggaa atcacaccaa gaaagaggga
    10501 accaacagtt gcaagacgac gaagaagttt agacatatat tcttctgtac ctttaccagg
    10561 acgaactcca tggatatagg caccactctt ttgtaggctc tctgctgctt tttcaggatt
    10621 aatctgtaca aacgtataga agaatgtaaa gagaataatc aacaaagcat acatggcaat
    10681 accagttgga gaagtagttg ccaacatctc ttgtgctacc cttacccaag cccaatcatg
    10741 acctgtggca ctcaaaaact gaagaatagc cgcaggcgct gcagtaatcg aactggcaaa
    10801 gataacaggg ataactccag cagggtttac ctttaacgga aggtaagagc tagatggagc
    10861 accttgtgca accttagtat attggattgg aattttgtat tctgcttgtt gaacataagt
    10921 tgtaaagtaa ataatcaaca atacagtaat aatcaaaatg attacgaaaa tgatagatga
    10981 agtgatacgg ctacttggga cgttcacaaa gtagtccaca tagatgccct gaatcatctc
    11041 tggaattgag gaaacaatcc cggcaaagat aatcatggaa acaccgtttc cgtatccctt
    11101 atctgtaatt tgctcaccca accaagtgac aatcatacta ccagctgtta agatgatacc
    11161 aatcgtcaga aaaacttgtg gagttaaagc agttttaatc aattgagctc cagccaaggt
    11221 attaaaacca gctgtaatcc cgatagattg cacaaaagcg agaactagag caatataacg
    11281 agtagcttga ttcaattttc ttcgacctac ttccccttgt ttaccccact ctacaaactt
    11341 gggtaaaata tccatttgca agagttggac aacaatagaa gcggtgatat agggactaac
    11401 tcctagggca aaaatcgaaa agttttttag ggcattcccc gacaccaagc tcaacatgtt
    11461 taagaaggat aatccactta aagcattcaa gctattggca ttcacaccag gaactgtaat
    11521 gctagttccg atacgaaaga ccaaaacgat aaaaattgta aataaaattt ttgatcgaac
    11581 ctgcttgact ttaagagctt ctcttaataa tttaaaaaac ataggtcacc tctcttagat
    11641 gacttctact gaaccacctt tagcagtgat agcttcttca gctgatttag agaatttagc
    11701 tgctttcaca gtcaatttct tagtcaactc accgttacca agaattttaa tacctgactt
    11761 ttcagcttta acaattcctg cttcgataag aacaactgga gttacttcag caccatcttc
    11821 aaagacgttc aattggtcaa ggttcacaat tgcgtattct ttagcgttga tgttagtgaa
    11881 tccacgtttt ggaagacgac ggaacaatgg agtttgtcca ccttcaaaac caaggcgaac
    11941 tccgccaccg ctacgagctt tttgaccttt ttgaccacga ccagatgttt taccgttacc
    12001 tgatgaagta ccacgaccaa cgcggttacg tactttacga gaaccttctg caggtttcaa
    12061 ttcatgaagt ttcattttta ttttctcctc ttttgtaaaa tgctagcgcc gataagggag
    12121 aaaaggttgt ctcccccatc aactcgccta tacgatgtca tcatagatga ctatatctag
    12181 ttttagggga tggtacagtg cacatcccct aaaacttcat tagtttactt cttcaactgt
    12241 tactaagtga gatactgctg taatcatacc acggatagca gcgttatctt ctttaataac
    12301 agagctgttc aatttgccaa gtccaagtgc tacaacagtt ttacgttgtg atggaatgcg
    12361 tccgattgga gacttagtca aagtaatttt aatttgagcc attttatccc ctttcttatg
    12421 ccaaatcaga aactgaaata ccacgaaggg cagcaatttc ttcagcgcgt ttcaattgtt
    12481 tcaaaccttc aacagttgca cgaacaatgt tgattggagt gttagaacca agtgatttag
    12541 atgtaatatc tgccacacct gccaattcca caacggcacg aactgcacca ccagcggcaa
    12601 ctccagaacc ttctacagca ggtttcaaca atactttagc tccaccgaat tctgaaagaa
    12661 cttcgtgtgg gattgttgtt ccaaccatag gaacttcgat caagtttttc ttagcatcat
    12721 ctactgcttt acggattgct tctggaactt cttgagcttt accagtacca aatcctacgc
    12781 gaccattgtg gtcaccaaca acaacaagag ctgcgaaacg aagacgacgt ccacctttaa
    12841 caacttttgt aacacggttg acagcaacta cgcgttcttc taattcaact gcattgtctt
    12901 taaatgccat tttctagtgt cctcctatta gaatttcaat ccgttttcac gagctgcatc
    12961 agccaaagct ttcacacgtc cgtgatatag atatccaccg cggtcgaaca ccacttctga
    13021 aataccttta gcgtttgcac gttctgcaac gagtttaccg acagcaacgg cttgttcagt
    13081 tttagttcct tttgaaactt ctttatcaag agttgaagca cttgcgagcg ttacacccgc
    13141 tacgtcatca atcacttgag cgtagatgcc tgtattagaa cggaacacgt tcaaacgtgg
    13201 gcgatcagca gttccagaga gttttccgcg aacgcgacgg tggcgttttt ggcggagttt
    13261 gtttttatct ggttttgaaa tcacagtttt cacctcttta gttttaaatc gtgtgctatg
    13321 cacaaagttg gaaaataggt tggtggttga tgatcaacca ctcaacatta tttacctgtt
    13381 ttaccttctt tacggcgaac gaattcacca acgtaacgga tacctttacc tttatatggt
    13441 tctggtgaac gaaggctacg tacgtaagca gctgtttgac caactacttc ttttgaaatt
    13501 ccgctaacaa cgattgttgt tgggtttgga agttcaaaag taattccttc tggagcttca
    13561 acttcgtctg gatgagattt accaacagcc aaaacaagtt tagatccttg aagctgtgca
    13621 cggtaaccaa ccccacgcat ttcaagttct ttcttgaatc cttctgatac accaacaacc
    13681 atgttgttca aaagggcacg agtagttccg tggatagttt tcatttcttt tgaatcgttt
    13741 ggacggtgaa gagttatttc agtaccttcc acacggattt caatatcttt tgagaactca
    13801 cgagtaagtt ctcctttagg tccttttaca gttacaacgt tgtcattgtt agcgagttca
    13861 acaccagcag gcaacacgat aactttatta ccaatacgtg acatgtttat tttctcctgt
    13921 taaattgtca ggccagaacg gccagttttc acggggttca gatacttatt tagttcaaga
    13981 gctgaactga acacgctcta agaactgtgc aaaatagata agttggcttg tttgtaaaca
    14041 aacttctcca acttcctaat tttgctttgt tctttacgag ctttgtatct tgattttacc
    14101 aaacgtaagc gataacctca ccaccaacat tcttttggcg tgcttcttta tcagtaagca
    14161 aaccttcaga agttgaaagg atggcaattc caagtccgtt aagaactttt ggaaggtctt
    14221 cacgtttttt gtagacacga agtcctggtt tagaaacacg tttcaagtta gtgataactt
    14281 tctcaccatt tggtccgtat ttaagaaata cacggatgac gccttgtttg tcatcttcaa
    14341 tgatttcaac gttttttaca aaaccttcgc gtttaaggat ttcagcaatc ccttttttga
    14401 tgtttgatgc aggtacttca agtacttcgt gtttagcttg gttagcatta cgaatacgag
    14461 ttaggaagtc tgcgattggg tcagtcataa ccattttgtt tttctcctct tactagtagt
    14521 ttgcaagttg cacttgctag ttaatacatg atacaaagag cgtaacagca cgagcaaaaa
    14581 tagacaattt aatgcagaag caatgctcca aagcaaatta gtcttttttg ccaaagctgt
    14641 agctcgtgtt caaattggca agcccaattg aacccgggct aaactctgtg tgaaaaagat
    14701 aaactttcct agaaacttta gtttcttcgt caagtttcct atttttactt ggagttttga
    14761 cgctcttgat atcttaaatt accaagatgc ttttgttaca ccaggaattt gacctttatg
    14821 cgcaagttcg cgaaaagcga tacgactcag accaaatttg cggtaaactg aatgtgggcg
    14881 ccccgtaacc ctacaacgat tatgtaaacg agtcggtgag gcgttgcgag gtaatttaga
    14941 taaaccttcg tagtcccctg ccgcctttaa tgcagcacgt ttttcagcat aacggtcaac
    15001 aattttttgg cgtttagcct ctctagctac cattgatttt ttagccatta gatttacctc
    15061 ctatattatt ttgcaaaagg cattccaagg cctgtaagca atgcacgtga ctcttcgtca
    15121 gtgttagcag ttgttacgat aacgatgtca agaccacgag ttttgtcaac gtcatcgaag
    15181 ttgatttctg ggaagattaa ttgttctttc acaccaagtg tgtagttccc gcgtccatca
    15241 aatgattttg ttgggacacc gtggaagtca cgtacacgtg gaagtgaaac tgataccaat
    15301 ttatccaaga attcgtacat acgttcacca cgaagggtaa cttttgcacc gatcgcaaca
    15361 ccttcacgaa gacggaagcc ggcgattgat tttttagctt tagtgataag tggtttttga
    15421 cctgagataa gtgccaattc ttcagcagct ttttcaaggc ttttagcgtt tgatacagct
    15481 tcaccaacac ccatgttcaa aacaatctta tctactttag gcacagccat cactgatgag
    15541 tagttgaatt gttctgtcaa agcaggaact acttcattaa gatatttttc ttttaaacga
    15601 tttgccatta tacttctcct ttccttcgtg attaatcaag cacttcgcct gattttttgt
    15661 tgtagcgaac ttttttaccg tctacaaatt tgtaaccaac acgaccagct acaccatttt
    15721 tgtccaaaac ttgaacattt gatacgtgga tagctgcttc tttctcgatg ataccacctt
    15781 gaggaagctc gttagttgga cgttggtgtt tcttaacaat gttaacacct tcaacgataa
    15841 ctttgtttac ttttggaagg gcagtaagga caacagcttc tgttccctta tctttaccag
    15901 cgattacgcg aactttgtcg ccttttttta caaacattag gtttctcctt gatttttctt
    15961 acgcccataa gggcacccta gcttgaagct aggggactag tttgttccta aaaattaaag
    16021 tacttctgga gcaagtgaca cgatcttcat gaaaccacct tcacgcaatt cacgtgcaac
    16081 tgggccaaag atacgtgttc cgcgaggagt tttgtcttca cggatgataa ctgctgcgtt
    16141 ttcgtcaaat ttgatgtatg aaccatcagc acgacgagca cctgatttag tacgaacgat
    16201 aactgcttta acaacgtcac cttttttaac cgcaccacca ggagtagctt gttttacaga
    16261 tgccacgata acatcaccga tgtttgcaaa tttacgtcct gaaccaccaa gaactttgat
    16321 agtcaagatt tcgcgagcac cgctgttgtc tgcgactttc aaacgagttt ctgtttgaat
    16381 catttcagtt ttctcctttc aggtttgatt agatgatgac cgcttcttca acaacttcta
    16441 caagacggaa acgttttgta gctgaaagcg ggcgagtttc catgatacgt acgatatcgc
    16501 cttctttggc aacattgttt tcatcatgag ctttgtattt tttagagtag ttaatacgtt
    16561 taccatagac tgggtggtta cgttttgttt caactacaac tgtgattgtc ttgtccattt
    16621 tgtcagatac aacacgtcca acaagaactt tacgattatt gcgttccatt gaaatttctc
    16681 cttccctagt ctattatttc gcttcagatt gaactgtttt gatacgagcg atttgttttt
    16741 taacttcttt caagcgagct gtttgttcca attgaccagt agcagcttgg aaacgaagtt
    16801 caaacaattc ttttttcaat tcgttttcgc gcttcgcgag ttcttcttga gaaagaccac
    16861 gaagttcttt aacaaattct tttacttcat taagtttcat gccttctcct tattctgctt
    16921 cacgttttac gaatttacat ttaactggca atttgtggct agcaagtcga agcgcttcac
    16981 gtgcaatctc ttcagataca ccagcgattt cgaacatcac tttaccacgt ttaactggtg
    17041 ctacccaacc ttcaggtgcc cctttaccag atcccatacg cacaccgata gctttagcag
    17101 tgtatgattt gtgtgggaag attttaatcc aaactttacc accacgtttc atgtaacgag
    17161 tcatggcgat acgagcagct tcgatttggc ggttagtgat ccagtggcta gttgtagctt
    17221 gaagaccgta ttcaccgaat gctacttctt ttccaccttt tgcttcaccg cgcatttttc
    17281 cacggaactc acgacggtgt ttaacacgtt taggtactaa cattggttat ttacctcctt
    17341 tagtgttttt acgagctgga agaacttcac cacggtagat ccatacttta acaccaagtt
    17401 taccgtatgt agtatctgct tcttcccaag cgtaatcgat atctgcacga agtgtgtgaa
    17461 gcggaacagt tccttcagag tatccttcag cacgggcgat atctgcaccg ttcaaacgac
    17521 ctgatacttg agttttgatt cctttagctc cagcacgcat tgcacgttgg attgcttgtt
    17581 tttgtgcacg acggaaagca acacgttgct ccaattgacg agcaattcct tcacctacaa
    17641 ggtgagcatc caaatcaggt tgtttgattt cgatgatgtt gatgtgtact tgttttccag
    17701 tcaatttgtt aagttttgca cggagtgcat caacgttagc accaccttta ccgataacca
    17761 tacctggttt agcagtgtga agtgaaacgt taactttgtt tactgcgcgt tcgatttcaa
    17821 tagttgaaac tgctgcgtca gcaagttctt tttgaacgaa tttacggatt gcaagatctt
    17881 catgaaggta atccgcgtat tctttttcag cataccattt ggcatcccaa tcacggatga
    17941 tgccgacacg cataccaatt ggatgtactt tttgacccac gattttacct ccttattttt
    18001 ctgcaacagc tacagtgatg tgagctgtac gtttgttgat tggtgaagct gaacctttcg
    18061 cacgtggacg gaaacgtttc atagttggtc cttcgtttgc gaatgcttca gatactacca
    18121 agttagcttt atccaaacca aagttgtttt cagcgttagc tacagctgag ttcaaaactt
    18181 tcaagatgat ttcagcagct ttgtttggag tgaatgtcaa gattgcgatt gcatcggcta
    18241 cgcttttacc acggatgtta tcaagaacaa gacgtgattt acgaggtgaa acacgtactg
    18301 tacgagccat tgctttagct gaagtaattt ctgccattta tgttctcctt attttctacg
    18361 tgttttcttg tcgtctgcag cgtgaccttt gtaagtacga gttggtgcaa attcaccaag
    18421 tttgtggcct accatgtctt cttggatgta aacaggtacg tgtttacgtc cgtcataaac
    18481 tgcaatagtg taaccaatga aacttgggaa gatcgttgaa cgacgtgacc aagttttaat
    18541 aacttttttc ttttcgtcgt tagcttgagc ttcaactttt ttcatcaaat gctcatcgac
    18601 gaaaggtcct tttttaagac tgcgtcccat ttttatattt tctcctttaa atgttgtacc
    18661 acagcggctt gcactcctat ggagcgctac cgagctggcg gatttactag ttgcttaagc
    18721 gactagttta atattatttc tcgttgcgac gacgaacgat aagtttgtca gatttcgctt
    18781 tcttgttacg agttttaaga ccaagagcag gtttgcccca tggagtagat ggtgctttac
    18841 gaccaactgg tgctttacct tcaccaccac cgtgtgggtg atcgttaggg ttcattacag
    18901 aaccacgaac tgttgggcgg atacctttcc aacggctacg tcctgcttta ccaaggttta
    18961 caagtccatg ttgttcgttt ccgacaacac caactgtagc acggcaagtt ccaagaatca
    19021 tacgaacttc acctgattga agacgaacaa gaacatattt accttcagaa cccaatactt
    19081 gagcagatgc accagcagca cgtaccaatt caccaccacg acctggtttc aactcgatgt
    19141 tgtgaatcaa agtaccaact gggatgttag caagtggaag agcgtttccg actttgatat
    19201 ctgcttctgg acctgaaacg atacgttgac ctacttcaag accttttgga gcgatgatgt
    19261 atgctttcac accgtcagtg tagtgtacaa gagcgatgtt tgcagaacgg tttggatcgt
    19321 actcgattgt tttaacaact gcttcaacgt tgtctttatt acgtttgaag tcaaccaaac
    19381 ggtagaaacg tttgtgtcca ccaccttggt gacgaactgt gatacgaccg ttgttgttac
    19441 gaccagcctt gctcttcaat gcaacaagca atgatttttc aggagtgctt gttgtgattt
    19501 cagcgaaatc caaagaagtc atattacggc gaccgtttgt tgttggttta taaacacgaa
    19561 ttcccacgat atttcctcct tagattattc agcttcagca gcaaacaact cgattgcttt
    19621 agaatcagct gtaagtgtga tgatagcttt tttagttttg ttagtaaaac cagtgtaacg
    19681 tccaacacgt ttagcttttg gttttacgtt gattgtgtta acattggcaa ctttaacacc
    19741 ttcgaaagca gcttcaacag cttgcttgat caaaagtttg tgtgcacgag tgtcaacttc
    19801 aaatacatat ttccctgctt caagttgagc cattgagctt tcagtgatga caggtttttt
    19861 gataacatca tacaaattca ttatgcaaga acctcctcga ttttagagat agctgcttgt
    19921 gtgacaagaa gtttgtcgct atttgcgatg tcaagaacac ttgcagttgt agcagttgca
    19981 actttcacgt ttggaaggtt acgagctgaa agagctgcga attcatttcc ttcttcaagg
    20041 ataacaagaa ctttagaatc gatgctcaat gctgcaagaa cttttgcaaa ttcagcagtt
    20101 tttggagctg taaatgaaag agcgtctaca gctacgaatt tgttttcagc aactttttca
    20161 gagtaaacta atttaagagc taggcgacga actttttgtg gaagtttgta gccgtatgaa
    20221 cgtggagttg gtccgaagac aacaccacca ccacgccatt gtggtgagcg gatagaacct
    20281 tgacgagcac gtccagttcc tttttgacgc catggtttgc gtccaccacc tgatactgca
    20341 gagcggtttt taacagcgtg tgttccttga cgaaggcttg cgcgttggct gatgattaca
    20401 tcaaacacaa ctgattcatt tggttcgata ccaaatactg catcgttaag aacaacttgg
    20461 ccagcttctt taccagtttg gtcaaataat gttacgtttg ccattgtgac tgatttcccc
    20521 tttctttatt atttaccagc tttaactgct gatttgatag tgataagaga tttcttagca
    20581 cctggtacgt tacctttgat aaggataacg ttcttttctg gaacaacttg tacaacttca
    20641 aggttttgaa ttgttacgcg gtcgccaccc atacgtcctg caaggttttt acctttgaat
    20701 acgcggttag gtgcaacagg ccccatagaa cctggacgac ggtggtaacg agaaccgtga
    20761 gccattggtc cacgtgattg tccgtggcgt ttgataacac cttggaaacc tttaccttta
    20821 gaagtaccag ttacatcaac aacatctcca gctgcgaatg tttcaactgt gatttcagcg
    20881 ccaacttcca agccttcaac gtttttgaat tcacgaatga agcgcttagg agccgtgtta
    20941 gctttcgcta catgtccttt agcaggtttg ttgctcaata cttcgcgttt gtcatcgaaa
    21001 ccaacttgga tagcgttgta tccgtctgtt tcaacagttt taacttgaag aacaacgttt
    21061 ggagttgctt caataactgt tacagggatc aattcgccag cttcagtgaa gatttgagtc
    21121 attcccactt ttttccctaa gattcctttt gtcatgagaa aatagttcct tttctatatt
    21181 ttttattcaa aaagttttta acgagcgttt tttatgctca agtctaagat acaaagggcg
    21241 tcaaactcaa agtgaaaata ggaaactaac gcagtgtcta gcggacaata ggaagtttat
    21301 ctttttcaca ccgagtttag cccgtgttca attagattga aacaccagcc tctgctcttt
    21361 tatattttag attaaagttt gatttctacg tttacaccac ttggaagatc caatttcatc
    21421 aaggcatcaa ctgttttttg agttgggtta acgatatcga tcaaacgttt gtgtgtacgc
    21481 atttcaaatt gttcgcgaga gtctttgtat ttgtgagtcg cacgaatgat tgtgtagagg
    21541 ctacgttcag ttggaagtgg gattggaccc gcaacttgtg cacctgtacg agtagctgat
    21601 tctacgattt ttgcagccgc tgtgtcaagc gtacggtgtt cgtaagcttt caaacggata
    21661 cggatttttt tgtttgccat ctttttctcc ttttcgtcta tttaagataa taggctagct
    21721 ccacaagaaa accgacgcgg tgttgcgtgg caatgcaacc gagcgtgtcg caacctcttg
    21781 catcaaaact aaggctgtaa tttacagcac cataatagaa taacacaaat cccctgcgat
    21841 tgcaagggat ttgttaggtt ttttcgtttt tttgggatga aactgttttc ctactttttc
    21901 tttctaaaat tagggatttt cttctatatt tcttctatac ataagttaag tcctcttgac
    21961 atcaaattta tcttctgatt ggtccactaa gatatccgcc ttagactcag tttctttata
    22021 gtatcgcaga tactgctccc gtctcatctg atggctagcc aatataaagg atgcatagcg
    22081 atttctcaca gtcgtatctc tagcaaggcg tcgctttaat tcggtctcct catccgtgta
    22141 gaaacagatg gttttttcaa agagttcctt gggtagaaaa ccaacagaca tcccttcgac
    22201 aatcaaaatt ggtttggctc cagacaagac ctcactagcc ttccaaggtt cttcaattgt
    22261 caagacatcc atacccgcct gcaaggcaag gatatctctc tgcaaactct ccagttcatg
    22321 cgccactggc agactggctg tcaccttttg attcggcgcg tccttgggta ctaccaaata
    22381 gcgtccggag gtgatatagg gatctgtttc tagcaaattt a
//
LOCUS       DAVLRT010000027        22230 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLRT010000027 DAVLRT010000000
VERSION     DAVLRT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.027"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..875
                     /locus_tag="VXM85_001020"
     CDS             <1..875
                     /locus_tag="VXM85_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345615.1"
                     /translation="EGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQ
                     AMYPLNIKAHTLEFPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAH
                     LYPVSKVTFAISSLGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQE
                     VTIGKIQDVLFPGLLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQT
                     IQPLQRIAHLIPFTYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFI
                     ERWGSSRKKEVFKV"
     misc_feature    1035..1166
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1182..1712
                     /gene="infC"
                     /locus_tag="VXM85_001021"
     CDS             1182..1712
                     /gene="infC"
                     /locus_tag="VXM85_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1345616.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            1745..1945
                     /gene="rpmI"
                     /locus_tag="VXM85_001022"
     CDS             1745..1945
                     /gene="rpmI"
                     /locus_tag="VXM85_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1345617.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            1997..2356
                     /gene="rplT"
                     /locus_tag="VXM85_001023"
     CDS             1997..2356
                     /gene="rplT"
                     /locus_tag="VXM85_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1345618.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            2414..2794
                     /locus_tag="VXM85_001024"
     CDS             2414..2794
                     /locus_tag="VXM85_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1345619.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            3044..3844
                     /locus_tag="VXM85_001025"
     CDS             3044..3844
                     /locus_tag="VXM85_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1345620.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            3807..4793
                     /locus_tag="VXM85_001026"
     CDS             3807..4793
                     /locus_tag="VXM85_001026"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1345621.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGAAAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            4893..7001
                     /gene="lytB"
                     /locus_tag="VXM85_001027"
     CDS             4893..7001
                     /gene="lytB"
                     /locus_tag="VXM85_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV1345622.1"
                     /translation="MKKVRFIFLALLFFLASPEGAMASDGTWQGKQYLKEDGSQAANE
                     WVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGYMAKSEWVEDKGAFYYLDQD
                     GKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKADGQHAEKEWLQIKGKDYYF
                     KSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFYIKENGNYADKEWIFENGHY
                     YYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDSHSQAWYYFKSGGYMAANEW
                     IWDKESWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDG
                     KIAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQ
                     AWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNKNAAYYQVVPVTANVYDSDGEKL
                     SYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRF
                     YHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEEL
                     DKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFF
                     GITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPY
                     WGEKIASVMMKINEKLGGKD"
     gene            complement(7062..8717)
                     /gene="pavA"
                     /locus_tag="VXM85_001028"
     CDS             complement(7062..8717)
                     /gene="pavA"
                     /locus_tag="VXM85_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1345623.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            8822..9319
                     /gene="ybeY"
                     /locus_tag="VXM85_001029"
     CDS             8822..9319
                     /gene="ybeY"
                     /locus_tag="VXM85_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1345624.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            9300..9695
                     /locus_tag="VXM85_001030"
     CDS             9300..9695
                     /locus_tag="VXM85_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1345625.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            9712..10611
                     /gene="era"
                     /locus_tag="VXM85_001031"
     CDS             9712..10611
                     /gene="era"
                     /locus_tag="VXM85_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1345626.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKIPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            10660..11484
                     /gene="mutM"
                     /locus_tag="VXM85_001032"
     CDS             10660..11484
                     /gene="mutM"
                     /locus_tag="VXM85_001032"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1345627.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            11484..12089
                     /locus_tag="VXM85_001033"
     CDS             11484..12089
                     /locus_tag="VXM85_001033"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1345628.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDTQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            12076..13275
                     /locus_tag="VXM85_001034"
     CDS             12076..13275
                     /locus_tag="VXM85_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV1345629.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            13275..13424
                     /gene="rpmG"
                     /locus_tag="VXM85_001035"
     CDS             13275..13424
                     /gene="rpmG"
                     /locus_tag="VXM85_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1345630.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            13464..13697
                     /gene="secG"
                     /locus_tag="VXM85_001036"
     CDS             13464..13697
                     /gene="secG"
                     /locus_tag="VXM85_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1345631.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            13799..16153
                     /gene="rnr"
                     /locus_tag="VXM85_001037"
     CDS             13799..16153
                     /gene="rnr"
                     /locus_tag="VXM85_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1345632.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     AGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYLEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRDSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            16116..16583
                     /gene="smpB"
                     /locus_tag="VXM85_001038"
     CDS             16116..16583
                     /gene="smpB"
                     /locus_tag="VXM85_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1345633.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKRQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            16599..17459
                     /gene="tehB"
                     /locus_tag="VXM85_001039"
     CDS             16599..17459
                     /gene="tehB"
                     /locus_tag="VXM85_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1345634.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMETVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            17532..18485
                     /gene="coiA"
                     /locus_tag="VXM85_001040"
     CDS             17532..18485
                     /gene="coiA"
                     /locus_tag="VXM85_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1345635.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIQQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            18504..20306
                     /gene="pepF"
                     /locus_tag="VXM85_001041"
     CDS             18504..20306
                     /gene="pepF"
                     /locus_tag="VXM85_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1345636.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            20308..21021
                     /locus_tag="VXM85_001042"
     CDS             20308..21021
                     /locus_tag="VXM85_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1345637.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEEFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            21088..22029
                     /gene="prsA"
                     /locus_tag="VXM85_001043"
     CDS             21088..22029
                     /gene="prsA"
                     /locus_tag="VXM85_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1345638.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(22146..>22230)
                     /locus_tag="VXM85_001044"
     CDS             complement(22146..>22230)
                     /locus_tag="VXM85_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1345639.1"
                     /translation="IDYYNNKRIKVKLKGLSPVQYRTKSFR"
BASE COUNT         6731 a         4102 c         4999 g         6398 t
ORIGIN      
        1 aagaagggcg ctggaaagag gcctactatc tgcagtggca agatgaagag aagaattatg
       61 aatttgtatc aaatgacccg actgctagct ctggcttaaa aatgggggtt gaccgcgaac
      121 ggaagattta ccaagccatg tatcccttga acataaaagc acatactttg gagtttccga
      181 cccacgggat tgatcagatt gtctggattt tagaggttat catcccaagc ttgtttgtgg
      241 ttgcgattat ttttatgcta acacaactat ttgcagaaag atatcaaaat catctggaca
      301 cagctcactt atatcctgtt tcaaaagtga catttgcaat atcctctctt ggagttggag
      361 tgggatatgt aactgtgctg tttatcggaa tctgtggctt ttcttttcta gtgggaagtc
      421 tgataagtgg ttttggacag ttagattatc cctacccaat ttatagctta gtgaatcaag
      481 aagtaactat tgggaaaata caagatgtat tatttcctgg cttgctctta gctttcttag
      541 cctttatcgt cattgtggaa gttgtgtact tgattgctta ctttttcaag caaaaaatgc
      601 ctgtcctctt tctttcactc attgggattg ttggcttatt gtttggcatc caaaccattc
      661 agcctcttca aaggattgca catctgattc cctttactta cttgcgttca gtggagattt
      721 tatctggaag attatctaag cagattgata atgtcgatct aaattggagc atggggatgg
      781 tcttacttcc ttgcctgatt atccttttgt tagtggggat tctatttatc gaaagatggg
      841 gaagttcacg taaaaaggaa gtttttaaag tttagttttc ctatagggaa gctaaagaaa
      901 aactaacata gggagagaat caacttggtt ctctcttttt gattcgaaaa ccaaaccaaa
      961 atacaaacac aaacttttca aaaaataact ttttatcttg acaagagcta gaaaacttgg
     1021 tatcatataa aagttgagaa aagcagaagt gagagcttct cgccttgtga cattaagttg
     1081 cctggcccta cggatgaaaa gtttcgaaga aacgctatca taacgtgcgg gcttgtatat
     1141 ttacgagtcc gctattgttt ttctctaata aaacaaaaga ggtgaaaacc atagcaaagc
     1201 aagacttatt catcaatgat gagattcgtg tacgtgaagt tcgcttgatt ggtcttgagg
     1261 gagaacagct aggcatcaag ccactcagtg aagcgcaagc tttggctgat aacgctaatg
     1321 ttgacctagt attgattcaa ccccaagcca aaccgcctgt tgcaaaaatt atggactacg
     1381 gtaagttcaa atttgagtac cagaagaagc aaaaagaaca acgtaaaaaa caaagcgttg
     1441 ttactgtgaa agaagttcgt ctaagtccaa caattgacaa gggtgacttt gatacaaaac
     1501 ttcgcaatgc acgtaagttc cttgaaaaag gaaataaagt taaggtatct attcgcttta
     1561 agggtcgtat gattacccac aaagagattg gtgcaaaagt tttagccgag tttgctgaag
     1621 caactcaaga tattgccatc atcgaacaac gtgctaaaat ggatggacgt caaatgttca
     1681 tgcaattggc gccagcgact gacaaaaaat aattgtcaga aagttaaata aaggagaaaa
     1741 aatcatgcca aaacaaaaaa cacaccgcgc atcagctaaa cgtttcaaac gtacaggttc
     1801 tggtggactt aaacgtttcc gtgcttacac ttctcaccgt ttccacggaa aaactaagaa
     1861 acaacgtcgt catcttcgta aagcatctat ggtgcattca ggagattaca aacgtatcaa
     1921 agcaatgctt actcgcttga aataatagct tgactgtaag taaacaattc taggaaatat
     1981 ttggaggaaa taaaacatgg cacgtgttaa aggtggcgtt gtatcacgca aacgtcgtaa
     2041 acgtattctt aaattagcaa aaggttacta tggagctaaa cacatcttgt tccgtactgc
     2101 aaaagaacaa gtaatgaact cttactacta tgcataccgt gaccgtcgtc agaaaaaacg
     2161 tgacttccgc aaattgtgga ttactcgtat caatgcggca gctcgtatga acggactttc
     2221 atactcacaa ttgatgcatg gtttgaaatt ggctgagatt gaagttaacc gtaaaatgct
     2281 tgctgacttg gctgttaacg atgcagtagc tttcacagct cttgcagatg cagctaaagc
     2341 aaaacttggt aaataacaac tgatgagact ggaacaggat tgtccagtct ttttaaaaat
     2401 aaaggagaaa gatatggctt caaaaatgct acacacttgc ttacgagtag aaaatcttga
     2461 aaaatcaatc gcattttatc aagatgcttt tggttttaaa gaattgcgtc gcagagattt
     2521 tccagaccat gccttcacga ttgtctatct aggtcttgag ggtgatgatt atgagttgga
     2581 gttgacttat aactacgatc acggtccgta tgtggttggt gatgggtttg cccatatcgc
     2641 cctcagtaca cctgatcttg aggcacttca tcaagaacac agtgcaaaag gctatgaagt
     2701 gactgagcca aatggtctac caggaactac accaaactat tactttgtca aagaccctga
     2761 tggatataag gtcgaagtca ttcgtgaaaa ataatttcgt gcaagttaag tagaatattc
     2821 gttttctact tgactttttt tagctgaaag agtatactaa taaaaattca acctttaagg
     2881 ggagtccaga gagactcaca aggtgtcaga taaaagaata gtgcaatttt ctagaggaga
     2941 ccttttgagt gtgctctctt gtgttgtacg attttaactg aggccttgca ctagcaaggt
     3001 cttttcttta tctggtcccc ttaaaattta aggaggaaaa gttatgaatc tcacatgtaa
     3061 gaagcgtttg ggtgtcattc ggttggaaac catgaaggtg gttgcacaag aggaaatcgc
     3121 gccagcaatc tttgaattag tcctagaagg agaaatggtt gaagccatgc gagcaggcca
     3181 atttcttcat ctgcgtgtac cagacgatgc ccatctctta cgtcgtccta tttcaatttc
     3241 gtctattgac aaggcaaaca agcagtgtca cctcatttat cggattgagg gagctgggac
     3301 tgcaattttt tcaaccttaa gtcagggaga cactcttgat gtgatggggc ctcagggaaa
     3361 tggttttgac ttgtctgacc ttgatgagca gaatcaggtt ctccttgttg gtggtgggat
     3421 tggtgttcca cccttgcttg aggtggccaa ggaattgcat gaacgtggag tgaaagtagt
     3481 gacagtcctc ggttttgcta ataaggatgc tgttattttg aaaacggaat tggctcagta
     3541 tggtcaggtc tttgtaacga cagatgatgg ttcttatggc atcaagggaa atgtttccgt
     3601 tgttatcaat gatttagata gtcagtttga tgctgtttac tcgtgtgggg ctccaggaat
     3661 gatgaagtat atcaatcaaa cctttgatga tcacccaaga gcctatttat ctctggaatc
     3721 tcgtatggct tgtgggatgg gagcttgcta tgcctgtgtt ctaaaagtac cagaaagcga
     3781 gacggtcagc caacgcgtct gtgaagatgg tcctgttttc cgcacaggaa cagttgtatt
     3841 ataaggagaa aattatgact acaaatcgat tacaagtgtc tctacctggt ttggatttga
     3901 aaaatccgat tattccagca tcaggctgtt ttggctttgg acaagagtat gccaagtact
     3961 atgatttaaa ccttttaggt tctattatga tcaaggcgac aacccttgaa ccacgttttg
     4021 ggaacccaac tccaagagta gcagagacgc ctgctggtat gctcaatgca attggcttgc
     4081 aaaatcctgg tttagaggtt gttttggctg aaaagctacc ttggctggaa agagaatatc
     4141 caaatcttcc tattattgcc aatgtagctg gtttttcaaa acaagagtat gcagctgttt
     4201 ctcatgggat ttccaaggca actaatgtaa aagctatcga gctcaatatt tcttgtccca
     4261 atgttgacca ctgtaatcat ggacttttga ttggtcaaga tccagatttg gcttatgatg
     4321 tggtgaaagc agctgtggaa gcctcagaag tgccagttta tgtcaaatta accccgagtg
     4381 tgaccgatat cgttactgtc gcaaaagctg cagaagatgc gggagcaagt ggcttgacca
     4441 tgatcaatac tctggttgga atgcgctttg acctcaaaac cagaaaacca atcttggcca
     4501 atggaacagg tggaatgtca ggtccagcag tctttccagt agccctcaaa ctcatccgcc
     4561 aagtagccca aacaacagac ctgcctatca ttggaatggg gggagtggat tcggctgaag
     4621 ctgccctaga aatgtatctg gctggggcag ctgctatcgg agttggaaca gctaacttta
     4681 ccaatcctta tgcctgccct gacatcatcg aaaatttacc aaaagtcatg gataaatacg
     4741 gtattagcag tctggaagaa ctccgtcagg aagtaaaaga gtctctgagg taaactgcaa
     4801 tcaatctgtt cttgattttt tattagtttg taatatgaat ttaggagaat tttggtacaa
     4861 taaatataaa taagaacaga ggaagaaggt taatgaagaa agtaagattt atttttttag
     4921 ctctgctatt tttcttagct agtccagagg gtgcaatggc tagtgatggt acttggcaag
     4981 gaaaacagta tctgaaagaa gatggcagtc aagcagcgaa tgagtgggtt tttgatactc
     5041 attatcaatc ttggttctat ataaaagcag atgctaacta tgctgaaaat gagtggctaa
     5101 agcaaggtga cgactatttt tacctcaaat ctggtggcta tatggccaaa tcagaatggg
     5161 tagaagacaa gggagccttt tattatcttg accaagatgg aaagatgaaa agaaatgctt
     5221 gggtaggaac ttcctatgtt ggtgcaacag gtgccaaagt aatagaagac tgggtctatg
     5281 attctcaata cgatgcttgg ttttatatca aagcagatgg acagcacgca gagaaagaat
     5341 ggctccaaat taaagggaag gactattatt tcaaatccgg tggttatcta ctgacaagtc
     5401 agtggattaa tcaagcttat gtgaatgcta gtggtgccaa agtacagcaa ggttggcttt
     5461 ttgacaaaca ataccaatct tggttttaca tcaaagaaaa tggaaactat gctgataaag
     5521 aatggatttt cgagaatggt cactattatt atctaaaatc cggtggctac atggcagcca
     5581 atgaatggat ttgggataag gaatcttggt tttatctcaa atttgatggg aaaatagctg
     5641 aaaaagaatg ggtctacgat tctcatagtc aagcttggta ctacttcaaa tccggtggct
     5701 acatggcagc caatgaatgg atttgggata aggaatcttg gttttacctc aagtttgatg
     5761 gcaaaatggc tgaaaaagaa tgggtctacg attctcatag tcaagcctgg tactacttca
     5821 aatccggtgg ttacatgaca gccaatgaat ggatttggga taaggaatct tggttttatc
     5881 tcaaatctga tgggaaaata gctgaaaaag aatgggtcta cgattctcat agtcaagctt
     5941 ggtactactt caaatccggt ggttacatga cagccaatga atggatttgg gataaggaat
     6001 cttggtttta cctcaagtct gatggcaaaa tggctgaaaa agaatgggtc tacgattctc
     6061 atagtcaagc ctggtactac ttcaaatctg gtggctacat ggcgaaaaat gagacagtag
     6121 atggttatca gcttggaagc gatggtaaat ggcttggagg aaaagctaca aataaaaatg
     6181 ctgcttacta tcaagtagtg cctgtcacag ccaatgttta tgattcagat ggtgaaaagc
     6241 tttcctatat atcgcaaggt agtgtcgtat ggctagataa ggatagaaaa agtgatgaca
     6301 agcgcttggc tattactatt tctggtttgt caggctatat gaaaacagaa gatttacaag
     6361 cgctagatgc tagtaaggac tttatccctt attatgagag tgatggccac cgtttttatc
     6421 actatgtggc tcagaatgct agtatcccag tagcttctca tctttctgat atggaagtag
     6481 gcaagaaata ttattcggca gatggcctgc attttgatgg ttttaagctt gagaatccct
     6541 tccttttcaa agatttaaca gaggctacaa actacagtgc tgaagaattg gataaggtat
     6601 ttagtttgct aaacattaat aatagccttt tggagaacaa gggcgctact tttaaggaag
     6661 ccgaagaaca ttaccatatc aatgcccttt atctccttgc ccatagtgcc ctagaaagta
     6721 actggggaag aagtaaaatt gccaaagata agaataattt ctttggcatt acagcctatg
     6781 atacgacccc ttacctttct gccaagacat ttgatgatgt ggataaggga attttaggtg
     6841 caaccaagtg gattaaggaa aattatatcg ataggggaag aactttcctt ggaaacaagg
     6901 cttctggtat gaatgtggaa tatgcttcag acccttattg gggcgaaaaa attgctagtg
     6961 tgatgatgaa aatcaatgag aagctaggtg gcaaagatta gtactataag tgaatatgat
     7021 ttgagtgaat agtaagttaa aaatcctgat ttcaagtaaa atcaggattt tttcatggat
     7081 gcaatttttt tggagtctgg tgtgacgcgg agggtctttt gtcctgtgta agtgacaaag
     7141 ccgggttttc caccagttgg tttattgagt tttttgactt caatcatatc tacctgcacc
     7201 agattcgaca ggcgcccttg agagaagtag gcagctaact ctgctgcgtc tgtcttgact
     7261 gcatcagatg ggtcaagatt tcctgagatg acaacatggc ttccaggaat gtccttagca
     7321 tggaaccaaa gttcctcctt gcgggccatt ttaaaggtca attcctcatt ttgaagattg
     7381 tttcgtccga catagatgat ggttttgcca tcgcttgcta gatattgttc tagttttttg
     7441 cgtttctgga ttttctcccg ttgtcttctg cggataaaac ctgtttgaat caattcttca
     7501 cggatttcag cgatttcttc cagtccagct tggttgagga cggtttctac actttccaga
     7561 tagagaatag tggctttggt ttcttcaatc aaatcagtca agtatttgac agcttctttg
     7621 agtttctgat accgtttaaa atagcgttgg gcattctggt tgggagtcag agccttatca
     7681 agcgcaatca tgataggttg gttggtatag tagttgtcta ggataacctg gtcttggtcg
     7741 ttaggcactt ggtgtaggaa ggttgtcagc aattctcctt tttgacgaaa ttcttcagcg
     7801 ttgtctgtcg ccagtaactc tttttcctgt tttttgagtt tgtgtcggtt tttctgaagt
     7861 tcattttcaa cacgacgaat cagttcactg gcctgctgtt tgacgcggtc gcgctcagcc
     7921 ttatccttat agtaggtgtc caacaaatca gaaagatttg caaaaggctc tcccacctga
     7981 tttgcaaaag gaactggact gaaggaagtc tcagtcaagc atggcttggt ttcttgattg
     8041 aaaaaatttc ggaaagcgga aagtttttca ctaaccagta tcctttccaa ttcatttgca
     8101 gtatcgcgtc ccagaccttg aaagaggctt tgaagatttt ttgctgttag ttcttgggtt
     8161 tgcaggattt caaagagctt ttcatctttg atagtaaaag gattgagaga ttctgtactt
     8221 ggcggagcga tataggtcga tcctggaagt aaggtgcggt agctattttg tgaaaaaccg
     8281 acgtgtttga taacttcgag gattttatga ctgcttttat cgaccagtag aatattactg
     8341 tgtttcccca taatttcaat aatcaaggta gcctggatat ggtctccaat ctcgttttta
     8401 ttggaaactg tcatttccac aatacggtca ttttccactt gctcaatcga ctcaatcagg
     8461 gccccctgca aatactttct caaaaccatg ataaaggtag aaggttgggc tggattttca
     8521 aaagtcgttt gggtcagctg aatgcgtcca aaaactggat gggcagaaag gagcaggcga
     8581 tggctttggc gattgctgcg gatttgcaag accaactctt gttcaaaagg ctgattgatt
     8641 ttctggatgc gaccattcac taattcgctt cgcaattcct caactatgtg gtgtaaaaaa
     8701 aatccatcaa atgacatcgt tctctccttg tgattgtatt ccatagtatt atatcaaaaa
     8761 ggtagaataa aatcatggaa atgtggtata ataaagccaa gtaaagagaa acgagaagca
     8821 catgtatatt gaaatggtag atgaaactgg tcaagtttca aaagaaatgt tgcaacaaac
     8881 ccaagaaatt ttggaatttg cagcccaaaa attaggaaaa gaagacaagg agatggcagt
     8941 cacttttgtg accaatgagc gtagtcatga acttaatctg gagtaccgtg acaccgaccg
     9001 tccgacagat gtcatcagcc ttgagtataa accagaattg gaaattgcct ttgacgaaga
     9061 ggatttgctt gaaaatccag aattggcaga gatgatgtct gagtttgatg cctatattgg
     9121 ggaattgttc atctctatcg ataaggctca tgagcaggcc gaagaatatg gtcacagctt
     9181 tgagcgtgag atgggcttct tggcagtaca cggcttttta catattaacg gctatgatca
     9241 ctacactccg gaagaagaag cggagatgtt cggtttacaa gaagaaattt tgacagccta
     9301 tggactcaca agacaataaa cgaaaatgga aaaatcgtga cctgatatcc agtttagaat
     9361 ttgctttgac aggtattttt actgctatca aggaagaacg aaatatgcga aaacatgcag
     9421 tgacggctct agtggttatc cttgcaggtt ttgtttttca ggtgtcacga atcgaatggc
     9481 tctttctcct attgagcatt ttcttggtag tagcctttga gattatcaac tctgctattg
     9541 aaaatatggt ggatttggcc agtcactatc acttttccat gctggctaaa aatgccaagg
     9601 atatggcggc tggtgcggta ttagtggttt ctcttttcgc agccttaaca ggcgcattga
     9661 tttttctccc gcgaatctgg gatttattat tttaaacagt aagaggaaat tatgacattt
     9721 aaatcaggct ttgtagccat tttaggacgt cctaacgttg ggaagtcaac ctttttaaat
     9781 cacgtcatgg ggcaaaagat tgccatcatg agtgacaagg cgcagacaac gcgcaacaaa
     9841 atcatgggaa tttacacgac tgataaggag caaattgtct ttatcgacac accagggatt
     9901 cacaaaccta aaacagctct cggagatttc atggttgagt ctgcctacag tacccttcgc
     9961 gaagtggaca ccgttctttt catggtgcct gctgatgaag cgcgtggtaa gggggacgat
    10021 atgattatcg agcgtctcaa ggctgccaag attcctgtga ttttggtggt gaataaaatc
    10081 gataaggtcc atccagacca gctcttgtct cagattgatg acttccgtaa tcaaatggac
    10141 tttaaggaaa ttgttccaat ctcagccctt cagggaaata acgtgtctcg tctagtggat
    10201 attttgagtg aaaatctgga tgaaggtttc caatatttcc cgtctgatca aatcacagac
    10261 catccagaac gtttcttggt ttcagaaatg gttcgcgaga aagtcttgca cctaactcgt
    10321 gaagagattc cgcattctgt agcagtagtt gttgactcta tgaaacgaga cgaagagaca
    10381 gacaaggttc acatccgtgc aaccatcatg gtcgagcgcg atagccaaaa agggattatc
    10441 atcggtaaag gtggcgctat gcttaagaaa atcggtagca tggcccgtcg tgatatcgaa
    10501 ctcatgctag gagacaaggt cttcctagaa acctgggtca aggtcaagaa aaactggcgc
    10561 gataaaaagc tagatttggc tgactttggc tataatgaaa gagaatacta agtagaggta
    10621 ggctcatgcc tgcttcttgt ttttacagaa ggaggactta tgcctgaatt acctgaggtt
    10681 gaaaccgttt gtcgtggctt agaaaaattg attataggga agaagatttc gagtatagaa
    10741 attcgctacc ccaagatgat taagacggat ttggaagagt ttcaaaggga attgcctagt
    10801 cagattatcg agtcaatggg acgtcgtgga aaatatttgc ttttttatct gacagacaag
    10861 gtcttgattt cccatttgcg gatggagggc aagtattttt actatccaga ccaaggacct
    10921 gaacgcaagc atgcccatgt tttctttcat tttgaagatg gtggcacgct tgtttatgag
    10981 gatgttcgca agtttggaac catggaactc ttggtgcctg accttttaga cgcctacttt
    11041 atttctaaaa aattaggtcc tgaaccaagc gaacaagact ttgatttaca ggtctttcaa
    11101 tctgcccttg ccaagtccaa aaagcctatc aaatcccatc tcctagacca gaccttggta
    11161 gctggacttg gcaatatcta tgtggatgag gttctctggc gagctcaggt tcatccagct
    11221 agaccttccc agactttgac agcagaagaa gcgactgcca ttcatgacca gaccattgct
    11281 gttttgggcc aggctgttga aaaaggtggc tccaccattc ggacttatac caatgccttt
    11341 ggggaagatg gaagcatgca ggactttcat caggtctatg ataagactgg tcaagaatgt
    11401 gtacgctgtg gtaccatcat tgagaaaatt caactaggcg gacgtggaac ccacttttgt
    11461 ccaaactgtc aaaggaggga ctgatgggaa aaatcatcgg aatcactggg ggaattgcct
    11521 ctggtaagtc aactgtgaca aattttctaa gacagcaagg ctttcaagta gtggatgccg
    11581 acgcagtcgt ccaccaacta cagaaacctg gtggtcgtct gtttgaggct ctagtacagc
    11641 actttgggca agaaatcatt cttgaaaacg gagaactcaa tcgccctctc ctagctagtc
    11701 tcatcttttc aaatcctgaa gagcaaaaat ggtctaagca aattcaaggg gagattatcc
    11761 gtgaggaact ggctactttg agagaacagt tggctcagac agaagagatt ttcttcatgg
    11821 atattcccct actttttgag caggactaca gcgattggtt tgctgagact tggttggtct
    11881 atgtggaccg agatacccaa gtagaacgct taatgaaaag ggaccagttg tccaaggatg
    11941 aagctgagtc tcgtctggca gcccagtggc ctttagaaaa aaagaaagat ttggccagcc
    12001 aggttcttga taataatggc aatcagaacc agcttcttaa tcaagtgcat atccttcttg
    12061 agggaggtag gcaagatgac agagattaac tggaaggata atctgcgcat tgcctggttt
    12121 ggtaattttc tgacaggagc cagtatttct ttggttgtac cttttatgcc catcttcgtg
    12181 gaaaatctag gtgtagggag tgagcaagtc gctttttatg caggcttagc aatttctgtc
    12241 tctgctattt ccgcggcgct cttttctcct atttggggta ttcttgctga caaatacggc
    12301 cgaaaaccca tgatgattcg ggcaggtctt gctatgacta tcactatggg aggcttggcc
    12361 tttgtcccaa atatctattg gttaatcttt cttcgtttac taaacggtgt atttgcaggt
    12421 tttgttccta atgcaacggc actgatagcc agtcaggttc caaaggagaa atcaggctct
    12481 gccttaggta ctttgtctac aggcgtagtt gcaggcactc taactggtcc ctttattggt
    12541 ggctttatcg cagaattatt tggcattcgt acagttttct tactggttgg tagttttcta
    12601 tttttagctg ctattttgac tatttgcttt atcaaggaag attttcaacc agtagccaag
    12661 gaaaaggcta ttccaacaaa ggaattattt acctcggtta aatatcccta tcttttgctc
    12721 aatctctttt taaccagttt tgtcatccaa ttttcagctc aatcgattgg ccctattttg
    12781 gctctttatg tacgcgactt agggcagaca gagaatcttc tttttgtctc tggtttgatt
    12841 gtgtccagta tgggcttttc cagcatgatg agtgcaggag tcatgggcaa gctaggtgac
    12901 aaggtgggca atcatcgtct cttggttgtc gcccagtttt attcagtcat catctatctc
    12961 ctctgtgcca atgcctctag cccccttcaa ctaggactct atcgtttcct ctttggattg
    13021 ggaaccggtg ccttgattcc cggggttaat gccctactca gcaaaatgac tcccaaagcc
    13081 ggcatttcga gggtctttgc cttcaatcag gtattctttt atctgggagg tgttgttggt
    13141 cccatggcag gttctgcagt agcaggtcaa tttggctacc atgctgtctt ttatgcgacg
    13201 agcctttgtg ttgcctttag ttgtctcttt aacctgattc aatttcgaac attattaaaa
    13261 gtaaaggaaa tctagtgcga gtaaaaatta atctcaaatg ctcctcttgt ggcagtatca
    13321 attacctaac cagtaaaaat tcaaaaaccc atccagacaa gattgaggtt ttaaagtatt
    13381 gtcccaagga aagaaaagta accctacatc ttgaatctaa gtagatttta tggtaaaata
    13441 atagggattt taaggagttt gatatgtata acctattatt aaccatttta ttagtattat
    13501 ctgttgtgat tgtgattgca attttcatgc aaccaaccaa aaaccaatcc agcaatgtat
    13561 ttgatgccag ttcaggtgat ttgtttgaac gcagtaaagc tcgcggtttt gaagctgtaa
    13621 tgcagcgttt gacagggatt ttagtctttt tctggctagc cattgcctta gcattgacgg
    13681 tattatcaag tagataagaa aataatgggc aggactaggt ctttgcctct ttttattttt
    13741 aaaggatgtt tgagaaggtt ttacagtaaa agaaaattaa aaaatctaga aagaaaatat
    13801 gaaagataga ataaaagaat atttacaaga caagggaaag gtgactgtta atgatttggc
    13861 tcaggctttg ggaaaagaca gttccaagga ttttcgtgag ttgattaaaa ccttgtcctt
    13921 aatggaaaga aagcaccaaa ttcgttttga agaagatggt agtctgacat tagaaattaa
    13981 gaaaaaacat gagattaccc tcaaggggat ttttcatgcc cataaaaatg gctttggctt
    14041 tgttagtctg gaaggcgagg aggacgacct ttttgtaggg aaaaatgatg tcaactatgc
    14101 tattgctggt gataccgtcg aggtagtgat taagaaagtc gctgaccgca ataagggaac
    14161 agcagcagaa gccaaaatta ttgatatcct agaacacagt ttgacaacag ttgtcgggca
    14221 aatcgttctg gatcaggaaa aacctaagta tgctggctat attcgttcaa aaaatcagaa
    14281 aatcagtcaa ccgatttatg ttaagaaacc agccctaaaa ttagaaggaa cagaagttct
    14341 caaagtcttt atcgataaat acccaagcaa gaaacatgat ttctttgtcg cgagtgttct
    14401 cgatgtggtg ggacactcaa cggatgtcgg aattgatgtt cttgaggttt tggaatcaat
    14461 ggacattgta tccgagtttc cagaagctgt tgttaaggaa gcagaaagtg tgcctgatgc
    14521 tccgtctcaa aaggatatgg aaggtcgtct ggatctaaga gatgaaatta cctttaccat
    14581 tgacggtgcg gatgccaagg acttggacga tgcagtgcat atcaaggctc tgaaaaatgg
    14641 caatctggag cttggggttc acatcgcaga tgtttcttat tatgtgaccg aggggtctgc
    14701 ccttgacaag gaagccctta accgtgcgac ttctgtttac gtgacagacc gagtggtgcc
    14761 aatgcttcca gaacgactat caaatggcat ctgctctctc aatccccaag ttgaccgcct
    14821 gacccagtct gctattatgg agattgataa acatggtcgt gtggtcaact ataccattac
    14881 acaaacagtt atcaagacca gttttcgtat gacctatagc gatgtcaatg atatcctagc
    14941 tggcgatgaa gaaaagagaa aagaatatca taaaattgta ccaagtatcg aactcatggc
    15001 caagcttcat gaaactttag aaaacatgcg tgtgaaacgt ggagctctca attttgatac
    15061 caatgaagcg aagattttag tggataaaca aggtaagcct gttgatatcg ttcttcggca
    15121 gcgtggtatt gccgagcgga tgattgagtc ttttatgttg atggctaatg aaacagttgc
    15181 cgaacatttc agcaagttgg atttgccttt tatctatcga attcacgagg agcctaaggc
    15241 tgaaaaggtt cagaagttta ttgattatgc ttcgagtttt ggcttgcgca tttatgggac
    15301 tgccagtgag attagccagg aggcacttca agacatcatg cgtgctgttg agggagaacc
    15361 ttatgcagat gtattgtcca tgatgcttct tcgctctatg cagcaggctc gttatttgga
    15421 gcacaatcac ggccactatg gactagctgc tgactattat actcacttta ccagtccaat
    15481 tcgtcgttat ccagaccttc ttgttcaccg tatgattcgg gattacggcc gttctaagga
    15541 aatagcagag cattttgaac aagtgattcc agagattgcg acccagtctt ccaaccgtga
    15601 acgtcgtgcc atagaagctg agcgtgaagt cgaagccatg aaaaaggctg agtatatgga
    15661 agaatacgtg ggtgaagagt atgatgcagt tgtatcaagt attgtcaaat tcggtctctt
    15721 tgtcgaattg ccaaacacag ttgaaggctt gattcacatc actaatctac ctgaatttta
    15781 tcatttcaat gagcgtgatt tgactcttcg tggagaaaaa tcaggtatca ctttccgagt
    15841 gggtcagcag atccgtatcc gtgttgaaag agcggataaa atgactggag agattgattt
    15901 ttcattcgta cctagtgagt ttgatgtgat tgaaaaaggc ttgaaacagt ctagtcgtag
    15961 tggcagaggg cgtgattcaa atcgtcgttc ggataagaag gaagacaaga gaaaatcagg
    16021 acgctcaaat gataagcgta agcattcaca aaaagacaag aagaaaaaag gaaagaaacc
    16081 tttttacaag gaagtagcta agaaaggagc caagcatggc aaagggcgag ggaaaggtcg
    16141 tcgcacaaaa taaaaaggca cgccacgact atacaatcgt agatacgcta gaggcaggga
    16201 tggtcctgac tggaactgaa atcaagagtg tacgagctgc tcgaattaat ctcaaggatg
    16261 gctttgctca agtgaaaaat ggagaagttt ggctgagcaa tgttcatatc gcgccttacg
    16321 aagagggcaa tatctggaac caggaaccag aacgtcgtcg taaactcctg ctccataaaa
    16381 ggcaaattca aaaattggaa caagagacca aagggacagg aatgacctta gttcccctta
    16441 aggtctatat aaaagatggc tacgctaagc ttcttttagg acttgccaaa gggaagcatg
    16501 actatgacaa acgggagtct atcaaacgtc gtgagcaaaa tcgagatatc gcgcgtgtga
    16561 tgaaagctgt taatcagcga taaaaagagg aattgaaaat ggaaaaatta gttgcctata
    16621 aacgcatgcc tttgtggaat aaacaaacaa tgcctgaagc tgttcagcaa aagcacaata
    16681 caaaagttgg gacttggggg aaaattactg tcttgaaggg agctctcaag tttattgaat
    16741 tgacagaaga aggggaagtt ctagctgaac acctctttga agcaggggca gacaatccaa
    16801 tggcccaacc tcaagcctgg caccgagtgg aagctgccac agatgatgtg gaatggtact
    16861 tggaatttta ttgtaaacct gaggattatt ttgctaaaaa atacaatacc aatcctgttc
    16921 attcagaggt cctagaggcc atggagacag tgaaacaagg gaaagctttg gatttgggtt
    16981 gtggtcaggg gcgtaattct ctttttctag cccagcaaga ttttgatgtg acggctgtag
    17041 atcaaaatgg actagctctt gaaatcttgc aaagcattgt ggagcaggaa gatttggaca
    17101 tgcctgttgg cctttacgat atcaattcag ctagcattga acaagaatat gattttatcg
    17161 tttcaacagt tgttctcatg tttctacaag cggaccgcat tccagctatt attcaaaata
    17221 tgcaggagaa aaccagtgtt ggtggttaca accttatcgt ttgtgccatg gacacggagg
    17281 attatccttg ctcggttaac ttcccattca cctttaaaga aggagaactg gcagactatt
    17341 acaaggattg ggaattggtt aagtacaatg aaaatccagg ccatttgcat cgtcgcgatg
    17401 agaatggcaa tcgtattcaa ctacgctttg cgaccttact agctaagaaa atcaagtaaa
    17461 cacacatgaa gattaggaat tttcctgatc ttttttcttt tttacgaatg atatagaaaa
    17521 ggagggaatt catgtttgtt gcgagagatg ctaggggaga attggtaaat gtgttagagg
    17581 ataaacttga gaagcaagca tacacctgcc cagcttgtgg aggccagctc catttgcgtc
    17641 aaggaccaag tgtacggacg cattttgccc ataaatcctt aaaagactgt gattttttct
    17701 ttgaaaatga aagtccagaa cacctggcca ataaggaatc cctctatcac tggttgaaaa
    17761 aagagacaaa ggttcaatta gagtacccgc tttcagaact taaacagatt gcggatgtat
    17821 ttgtaaatgg caatctagct ctagaagttc agtgtagtcc cttgcctcag aaagtcctta
    17881 aagagcgaag tgagggctat cgtagtcagg gttaccaagt actgtggttg ctgggtcaaa
    17941 aactgtggct caaggagcgt ttgactcgtc tacagcaagg ttttctttat ttcagtcaaa
    18001 acatgggctt ttatgtttgg gaattagaca aggaaaaaca agttttaaga ctcaaatacc
    18061 tgatttacca ggatctccgc ggtaaactcc attatcaaat caaggaattt tcctatggtc
    18121 aaggtagttt attggaaata ttgcgtcttc cctataagag acaaaaaata tctcatttta
    18181 cagtttctga ggacaaggac atctgtcgct atatccagca acaactttat tatcaaaatc
    18241 tcttttggat gaaagaacaa gcagaagcct atcaaaaggg agaaaatatc ctgacttatg
    18301 gactgaaaga atggtatcca caaattcgac caatagtggg caaatttttc cagattgaac
    18361 aagacttgac tagctattat cagcactttt atacctatta ccaaaaaaat cctcaaaatg
    18421 attggcaaaa gctttatcca ccagcctttt atcagcaata tttcttgaaa aatatggtag
    18481 aatagaaagg atggaggaat ctaatggtat tacaaagaaa tgaaataaat gaaaaagata
    18541 catgggatct atcaacgatc tacccaactg accaggcttg ggaagaagcc ttaaaagatt
    18601 taacagaaca attggagaca gtagcccagt atgaaggcca tctcttggat agtgcggata
    18661 acctactaga aatcactgaa ttttctcttg aaatggaacg ccagatggag aagctttacg
    18721 tttatgctca tatgaagaat gaccaggata cacgtgaagc taagtatcaa gagtactatg
    18781 ccaaggccat gacactctac agccagttag accaagcctt ttcattctat gatcctgaat
    18841 ttatggagat tagcgaaaag cagtatgctg actttttaga agctcaacca aagctgcagg
    18901 tttatcaaca ctattttgac aagctcttgc aaggcaagga tcacgttctt tcacaacgtg
    18961 aagaagaatt attggctgga gctggagaaa tctttggttc agcaagtgaa accttcgcta
    19021 tcttggacaa tgcggatatt gtgttccctt atgtcctaga cgatgatggt aaagaagttc
    19081 agctatctca tgggacttac acacgtttga tggagtctaa aaaacgtgag gttcgccgtg
    19141 gtgcctatca agctctttat gcgacttacg aacaattcca acacacctat gccaaaacct
    19201 tgcaaaccaa tgttaaggtg caaaattacc gtgctaaagt tcgtaactac aagagtgctc
    19261 gtcatgcagc cctcgcagcg aattttgttc cagaaagtgt ttatgacaat ttggtagcag
    19321 cagttcgcaa gcatttgcca ctcttacatc gctatcttga gcttcgttca aaaatcttgg
    19381 ggatttcaga tctcaagatg tacgatgtct acacaccgct ttcatctgtt gaatacaatt
    19441 ttacctacca agaagccttg aaaaaagcag aagatgcttt ggcagtcttg ggtgaggatt
    19501 acttgagccg tgtcaaacgt gccttcagcg agcgttggat tgatgtttac gaaaatcaag
    19561 gcaagcgttc aggtgcctac tctggtggtt cttacgatac caatgccttt atgcttctca
    19621 actggcagga caatctggac aatctcttta ctcttgttca tgaaacaggt cacagtatgc
    19681 attcaagcta tactcgtgaa actcagcctt atgtttacgg agattactct atctttttgg
    19741 ctgagattgc ctcaactacc aatgaaaata tcttgacgga gaaattattg gaagaagtgg
    19801 aagacgacgc aacacgcttt gctattctca ataacttcct agatggtttc cgtggaacag
    19861 ttttccgcca aactcaattt gctgagtttg aacacgccat tcaccaagca gatcaaaatg
    19921 gggaggtctt gacaagcgat ttcctaaata aactctacgc agacttgaac caagagtatt
    19981 atggtttgag taaggaagac aatcctgaaa tccaatacga gtgggctcgc attccacact
    20041 tctactataa ctactatgta tatcaatatt caactggctt tgcggccgcc tcagccttgg
    20101 ctgaaaaaat tgtccatggt agtcaagaag accgtgaccg ctatatcgac tacctcaagg
    20161 caggtaagtc ggactatcca cttaatgtca tgagaaaagc tggtgttgat atggagaagg
    20221 aagactacct caacgatgcc tttgcagtct ttgaacgccg tttaaatgag tttgaagccc
    20281 ttgttgaaaa attaggattg gcataaaatg gttgaatcgt atagtaagaa tgctaaccat
    20341 aacatgcgtc gtcctgtcgt caaagaagaa attgtagact tgatgcgtca gcgtcaaaag
    20401 caggtcacag gtttcttgaa agaattggaa gaatttgccc gcaaggaaaa tattcctatt
    20461 attccccatg aaacggttgc ttatttccgt tttcttatgg aaaccatgca gcctaaaaat
    20521 attctggaaa ttgggacggc tatcggtttt tcagctctct tgatggctga acatgcgcca
    20581 aatgctaaga ttacaactat tgatcgtaat ccagaaatga ttggttttgc caaggaaaat
    20641 tttgcccagt ttgacagtcg caagcaaatc actctcctag agggagatgc ggtggatgtc
    20701 ttatctacac tgacagagtc ttatgatttc gtctttatgg attctgccaa gtctaaatac
    20761 atcgtctttc tgccagaaat cctcaaacat ttggaagttg gtggtgtggt tgtcttggat
    20821 gatatttttc aaggtggtga tgttgccaag gatattatgg aagtccgtcg tggtcagcga
    20881 accatttatc gaggccttca aaaattattt gatgcaacct tagacaatcc agaactcacc
    20941 gcaacattag tgcctttagg agatggtatt ctcatgcttc gtaaaaatgt agcagatgtt
    21001 caactgtctg aaagcgaatg attttcagaa aaatttaaga aaaaatagta aaatagatag
    21061 agtaacactt atctcaaagg agtagacatg aagaaaaaat tattggcagg tgccatcaca
    21121 ctattatcag tagcaacttt agcagcttgt tcgaaagggt cagaaggagc agaccttatc
    21181 agcatgaaag gggatgtcat cacagaacat caattttatg agcaagtgaa aaacaaccct
    21241 tcagcccaac aagtcttgtt aaatatgacc atccaaaaag tttttgaaaa acaatatggc
    21301 tcagagcttg atgataaaga ggttgatgat actattgccg aagaaaaaaa acaatatggc
    21361 gaaaactacc aacgtgtctt gtcacaagca ggtatgactc ttgaaacacg taaagctcaa
    21421 attcgtacaa gtaaattagt tgagttggca gttaagaagg tagcagaagc tgaattgaca
    21481 gatgaagcct ataagaaagc ctttgatgag tacactccag atgtaacggc tcaaatcatc
    21541 cgtcttaata atgaagataa ggccaaagaa gttctcgaaa aagccaaggc agaaggtgct
    21601 gattttgctc aattagccaa agataattca actgatgaaa aaacaaaaga aaatggtgga
    21661 gaaattacct ttgattctgc ttcaacagaa gtacctgagc aagtcaaaaa agccgctttc
    21721 gctttagatg tggatggtgt ttctgatgtg attacagcaa ctggcacaca agcctacagt
    21781 agccaatatt acattgtaaa actcactaag aaaacagaaa aatcatctaa tattgatgac
    21841 tacaaagaaa aattaaaaac tgttatcttg actcaaaaac aaaatgattc aacatttgtt
    21901 caaagcatta tcggaaaaga attgcaagca gccaatatca aggttaagga ccaagccttc
    21961 caaaatatct ttacccaata tatcggtggt ggagattcaa gctcaagcag tagtacatca
    22021 aacgaatagt ccaaatcaat gagtcaggga aaaaaactcg acttcaggaa aaaatgaagc
    22081 aaacattccc aaaataaaac gcatagtaca aggtttgtac tgcccccaaa agttagacaa
    22141 ttaatttatc tgaaggattt agttctgtat tgcacagggc taagtccttt tagttttacc
    22201 ttaattctct tattgttgta ataatcaata
//
LOCUS       DAVLRT010000028        21312 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLRT010000028 DAVLRT010000000
VERSION     DAVLRT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.028"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            55..351
                     /locus_tag="VXM85_001045"
     CDS             55..351
                     /locus_tag="VXM85_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1345640.1"
                     /translation="MDTPDENGYVADNYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDLVSAGTGEMNKRYGFIYVDRDNVGNGVLKRSKKKSFYWYKDVIDSNGASIG"
     gene            472..1416
                     /gene="manA"
                     /locus_tag="VXM85_001046"
     CDS             472..1416
                     /gene="manA"
                     /locus_tag="VXM85_001046"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1345641.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(1437..2800)
                     /locus_tag="VXM85_001047"
                     /pseudo
     CDS             complement(1437..2800)
                     /locus_tag="VXM85_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3028..3777
                     /locus_tag="VXM85_001048"
     CDS             3028..3777
                     /locus_tag="VXM85_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1345642.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(3797..4252)
                     /locus_tag="VXM85_001049"
     CDS             complement(3797..4252)
                     /locus_tag="VXM85_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1345643.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4272..5447)
                     /locus_tag="VXM85_001050"
     CDS             complement(4272..5447)
                     /locus_tag="VXM85_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1345644.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDNQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5506..6351)
                     /locus_tag="VXM85_001051"
     CDS             complement(5506..6351)
                     /locus_tag="VXM85_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1345645.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6476..7033
                     /locus_tag="VXM85_001052"
     CDS             6476..7033
                     /locus_tag="VXM85_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1345646.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7052..7519
                     /locus_tag="VXM85_001053"
     CDS             7052..7519
                     /locus_tag="VXM85_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1345647.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7608..8237
                     /gene="upp"
                     /locus_tag="VXM85_001054"
     CDS             7608..8237
                     /gene="upp"
                     /locus_tag="VXM85_001054"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1345648.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8411..9001
                     /gene="clpP"
                     /locus_tag="VXM85_001055"
     CDS             8411..9001
                     /gene="clpP"
                     /locus_tag="VXM85_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1345649.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9080..9328
                     /locus_tag="VXM85_001056"
     CDS             9080..9328
                     /locus_tag="VXM85_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1345650.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9430..10590
                     /locus_tag="VXM85_001057"
     CDS             9430..10590
                     /locus_tag="VXM85_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1345651.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDSLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10858..11727
                     /locus_tag="VXM85_001058"
     CDS             10858..11727
                     /locus_tag="VXM85_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1345652.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11731..12687
                     /locus_tag="VXM85_001059"
     CDS             11731..12687
                     /locus_tag="VXM85_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1345653.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12687..13451
                     /locus_tag="VXM85_001060"
     CDS             12687..13451
                     /locus_tag="VXM85_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345654.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13451..14161
                     /locus_tag="VXM85_001061"
     CDS             13451..14161
                     /locus_tag="VXM85_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345655.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14469..15125
                     /locus_tag="VXM85_001062"
     CDS             14469..15125
                     /locus_tag="VXM85_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1345656.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIISL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15233..16328
                     /gene="prfB"
                     /locus_tag="VXM85_001063"
     CDS             join(15233..15304,15306..16328)
                     /gene="prfB"
                     /locus_tag="VXM85_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1345657.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16346..17038
                     /gene="ftsE"
                     /locus_tag="VXM85_001064"
     CDS             16346..17038
                     /gene="ftsE"
                     /locus_tag="VXM85_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1345658.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17031..17957
                     /locus_tag="VXM85_001065"
     CDS             17031..17957
                     /locus_tag="VXM85_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345659.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            18243..20423
                     /locus_tag="VXM85_001066"
     CDS             18243..20423
                     /locus_tag="VXM85_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1345660.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEV
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     NAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20475..21290
                     /locus_tag="VXM85_001067"
     CDS             20475..21290
                     /locus_tag="VXM85_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1345661.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
BASE COUNT         6526 a         4014 c         4645 g         6127 t
ORIGIN      
        1 tataatgctt tttgatttta gtttagtatt atcgtagaaa atggactcgg tgctatggat
       61 acaccggatg agaatggtta tgtagcagat aactatcgga ttacttactt agaggcccac
      121 atcaaggcca tgcgagatgc catttaccaa gacggggttg acttgcttgg ttatacgact
      181 tggggctgta tcgatctggt ttcagctggc acaggggaaa tgaataagcg ctatggcttt
      241 atctatgtag atcgagataa tgtaggaaac ggagttctca aacgtagcaa gaagaagtcc
      301 ttctattggt acaaggatgt cattgatagc aatggtgcaa gcatcgggta gagcacattt
      361 gtttactatt tttataaaat cttctccctg ctttataaga tgtaaaaaag agttcaattc
      421 gaactctttt tttgctataa tgaggggaga aaaatcagac aggagatcga catgtcagaa
      481 ccattatttt tacaatcagt tatgcaagaa aaaatctggg gtggagccaa gctacgtgat
      541 gagtttggct acgacatccc aagtgaaaaa atcggagaat attgggccat ctcagcccat
      601 ccaaatggag tctctaaagt tgccaatggt cgttacgagg gaacagatct tgctactttg
      661 tatgcggaac accgtgaatt atttggcaat cgtccagaac ctgtatttcc acttttgacc
      721 aagatcctcg atgccaacga ctggctcagt gtccaagttc acccagacga tgcttatgga
      781 ctcgagcatg aaggcgaact cggaaaaaca gaatgctggt acattatcgc agcggatgaa
      841 ggttcagaga ttatctacgg tcacaatgcc aagtcaaaag aagaactccg ccagcaaatc
      901 gaggataaga actgggatgc gctgttgaca aaagtgccag ttaaagcagg agatttcttc
      961 tatgtaccaa gcggtaccat gcacgctatc ggggcgggta tcttgattct tgaaacccag
     1021 cagtctagcg ataccactta ccgtgtctat gactttgacc gaaaggacga taagggcaac
     1081 ttgcgtgaac ttcaccttga aaaatctatc gatgtcttga acattggtga acccgcaaat
     1141 agccgtccag tgacaatcaa agcagatgat ctacgttcca ctctccttgt atccaatgat
     1201 ttcttcgcag tttacaaatg ggaaattact ggaaaagtta actttgaaaa gacagctgac
     1261 tacagcttgt tgagtgtctt ggctggtcaa ggtcaactaa ctgttgacgg gaaaaactat
     1321 ccaatccaaa aagggagaca ctttatccta ccaagtgatg ttgaatcttg gactttagaa
     1381 gggcaagatt tggaattgat tgttagtcat ccataaaaag aaagggcctg agttcactac
     1441 tcaagtcctt tttgattaca taaattgggc gatgaagacc acgatgatga ttggaatgac
     1501 gaaacgaaga aacgaaggag gaagagccag acttggaaaa gtacttgttt ccaggctctt
     1561 tcatcgagat ggagttcctc cataacaaga gcctttttaa agatatagcc tgtaaaaagt
     1621 gaaaggcaga gagctccaaa tggcatgaga agattggaaa ccaagaaatc catggcatca
     1681 aagaaagtct taccaaagat gtgaacatcc gccataactc cataggatag ggctgaagga
     1741 atgccaaaga caaaggtcaa aattcctaaa ataacactcc atttggcacg tttgctgtta
     1801 tcctggttgg tgatattgcc cacattgatc tccagcatca cgacagaaaa agtgactggc
     1861 gcaaaaagga agagcaagag gaaaaggacg tagaaaatgg ttccaaaagg catcttgtca
     1921 aagagttgag gcaagacgat aaagagcaag ctcggtcccc cttcagactg gatattgaag
     1981 ggggaccgag cttggaaaat ggctagacct gccatgatgg atatcgagat attcatggct
     2041 acgatggaga ttcctgactg gactaggttg gttttcttgt ccaagtaaga agcataggtc
     2101 aacatgactg taacccctag tgagagggca aagaaagatt gtcccagagc atagaggaga
     2161 ccagcgctag tcagttttga aaagtctggt ttgaggaagt aaagaacccc ttccatggca
     2221 tttggcaaac tgagagagcg accgatgata acaacaaaga tgataaagag caggggcatc
     2281 atgactttcg aagctctttc aatccctttt tgaaccccac gtgatacaat aaagatattt
     2341 aataagataa aggctgcttg agctcctagg gcaatggctg gatttgaaat gattgaagta
     2401 aataactgag cataatcacc cgttccacca agttggaaca atttcccaaa ctcaatacct
     2461 agatagacta gaatccatcc tccgataaca ctgtaaaaag ataagaggat aaagagggca
     2521 aaggcgccaa tccaaccgat aaagttgtac ttgttattct tgcccagttt tccaaaggtt
     2581 ttgatagcgg aaacgccagc actacggcca agggcaaact cagccagcag gagagggaaa
     2641 ccgattaaaa tagtggaaat gagaaagatt agtaaaaagc ctccatcgcc attagcagca
     2701 gtcatgtagg gaaacttcca aacggaacca agcccgatgg ccagccagca gatgctagaa
     2761 taaaaccaag tttcgacccc cattgtgatt tttctgacat agttaaactc tgatctcctt
     2821 tttcaaaata agaaaaagct acttgagttg tcaaatagcc ttctgtttga ttgatagact
     2881 tgactatcct atcatgtttt ctaaggtctg tcaagaaaat cattttcaag ttttcacacc
     2941 tttctcaaaa aagttaaaaa attttctcaa aaacgcttga ctctgaccta aggggagggg
     3001 ttatactatc attgtaagga ggaaatcatg taccatataa aagaagctgc gcagctttca
     3061 ggtgtctctg tcaagaccct gcatcactat gacaagatag gactcttggt ccccttaaag
     3121 tcggaaaacg gctatcgaac ctacagtcaa gaggatttgg aacgccttca ggtcattctt
     3181 tactacaaat atctaggctt ttctttagag aaaatagcag agctgttaaa ggaagaaagg
     3241 acagatttat tgccccattt gactaggcag ttggactatc taactcgcga aaggcaacat
     3301 ctggatacct tgatttccac cttgcaaaaa actattcaag aacaaaaagg agaaagaaaa
     3361 atgaccattg aggaaaaatt cacgggattt agctatcaag acaatcaaaa ataccaccaa
     3421 gaagcggtag agaaatatgg tcaagaagtc atgggacaag cgctcgaacg ccaaaaaggt
     3481 cacgaagacg aggctacggc cgcctttaac caagtctttc aaactttggc acaaaatctt
     3541 caagttggtt tacctgcaac agcaaccgaa aaccaagagc aagcagccaa gctcttgcaa
     3601 gccattcgca cttatggatt tgactgctct attgaggtat tcggtcatat cggtaaaggt
     3661 tacgtctata acccagagtt taaggaaaac attgacaagt ttggttctgg aacagcccag
     3721 tacacgtcag atgccattgc agcttacgtt cagacaaatg cagaaaaaat aggctaggaa
     3781 tttcctagcc tattttttac ttcaaatcat aaagccagtc gtcaccgttt ttgtagtaaa
     3841 agaattcact gagatcttct tctagaaaca cacgaagcat atcagacata tcatcggttg
     3901 caagttttag atgagaaaga ttttcaaagt cctcccacca aactttccct tcgtctgaag
     3961 actggagttc accagtaaag tgttctgtct tgtaaaaaag gacgacataa cgataatcct
     4021 tgtcgtcata ccagtttttg ataccacaga gttggggttt ggaaatgatc agaccagttt
     4081 cttctttcac ttcacgaatg acagcatcga caaaggattc gccacgttca acatgaccac
     4141 caggaaaagt aatgccaggc cagtcgggac taactcggtc ttggaccagg accttatctc
     4201 cgtttttaat catacacatg ttaacaaatt cgactgcctc tcttctgttc attcttcaca
     4261 acctttaatc tttaatcata atgcagactt cccgccaccc agccggtaca gagggcagaa
     4321 gtgatgttaa agccacccgt gtgggcattg atatccataa cttcgcctgc aaagtggagg
     4381 ccaggtacca gcttactttc aagggtttta ggattgattt ccttgagact gactccaccc
     4441 ttggtaacaa aggactttgc aagggacatt tttccagtta caggaatttt aagttcttta
     4501 atggactgga caagctgttc tcgttccttt tcagtcagct gtttgacttt ttcaggatat
     4561 ccttgtacaa aaaattcggc caagcgttct ggtaacaagg tttttaaagc gtttttcaag
     4621 gatttttccc gattttcttc tagaaatgta accaagtcct tctcagaaag ttgaggcaaa
     4681 acatcgagtg agagaacctc cccacctttg acaaagctag acatgcgtag ggcagcagga
     4741 cctgacaaac caaagtgggt aaagagtaaa tcatgagtga tgacatgctt accataactt
     4801 agggtcacat cgtccaaaga aatcccttgt aaggctttat gtggaaaatc tgttaataaa
     4861 ggactttcag cagcctcaag atcggtgatg gtatgcttaa aatggcgagc aatctcgtga
     4921 ccaaaaccag tcgaaccagt cgaaggataa gacttaccac ctgttgtgac aatgagtttc
     4981 tcacaagtga aggtttgatc cgctgactta aggacaaact ggttatctac ttttttaaca
     5041 gaaacgattt ctatttgagt agcaacttga ccacctagtt cggtgatttt cttttccaaa
     5101 gcttcgataa tagtccgaga cttgtcactg gctggaaaga cgcgtccgtg gtcttcgacc
     5161 ttaagtttaa caccattttc tgtaaaaaag ttgatgatgt catgattatc gaactgggag
     5221 aaaacactgt aaagaaagcg tccgtttcca ggaattccag ctagcaggtt gtctaagcta
     5281 ccattgttgg tcacattgca acgtccccca ccagtcccag ctaatttttt tccaagtttc
     5341 cgattttttt cgatgaggag ggttttctgt ccataaaagc tactggaaat cgtagccatc
     5401 ataccagcag gtcccccacc gatgacaata gtatcaaaat gtttcatagc tctattgtac
     5461 cacaaaaaaa caagagatga tggtcacctc ttgtcaagaa tgcaattaat caatttcata
     5521 gcccatcagc aaaccgccct cttctgcata gaaactgcag agaccagagg ttggtagaat
     5581 tttaatatcc gcttgtggga aggtttcacg gattcgctct gagagctgtt gacaacattt
     5641 ttcgttattg cgttgggcca tgacaatacg gccaccagca tatccagctt ttactaactc
     5701 atcataggca gcttgaactg atttctttga tccccttgct ttttgtagca attcgagagt
     5761 cccagtttca ctagcttttc cgaccatacg aatgttgaga aggccaacga ccgtaccgat
     5821 aagcttgctc aaacggccgt tcttcaccaa gttatcgact ttggctagga caaagagcaa
     5881 cttagttttt tcttgatagg cggtgatagc ttcaaccact tcttcaaaag acaagccctg
     5941 gtcaatcaag tcattcaatt tttctacgag taggtcaact tcaccaccag cagataaact
     6001 atcaatcaca tgaatcttag tgtcaggatg gtcttccaga taaatattct ttgctagttg
     6061 agcactattg tgactgccag aaagggtacc tgtgatggtt actaggaaaa tgtttttggc
     6121 accttcaaat gctcgcaaat agtcatctgg gcttggacaa gctgattttg aagcttctgc
     6181 agttgcatac atggtttcca tcatttggtc aatatcgaga ctggcgtcat caacaaagac
     6241 ctgatcagct acttgaatgg ttaaggggac acttacaaag gttgtgttaa tagctggtgt
     6301 tggcagttga cgataatcac aaccagagtc agcaataatc ttccaagtca tagaaattct
     6361 ccatctttgt cagttataca ttgacaaagg ttctgtcttt ttttacaatt atatcatgaa
     6421 aactcttgaa acaaaagcat catccgtctg ttgtgacaag tagaaagaaa atgttatgtc
     6481 tgaacgtaga atctctgaaa agtctcttga aaatctcaga aaatcaaatc aagaatccaa
     6541 tttattaacc agagaagcca ttgaaacagc cctcttgcaa ctcttggaaa aaaaggagct
     6601 gaccaagatt agtatttctg aattggtcaa acgtgcaggt gtttcgcgtg cggcctttta
     6661 tcgcaattat gactccaaag aggaaatttt agagagcgtc tttaaacgaa ctgtccacaa
     6721 tattatggaa cagctgcatc attacgattt aaagacagac ctttatttgg tttgggttca
     6781 ccttttccgg gaggccagaa aggaagctag agtaattcaa ttggccttgg attaccatct
     6841 ggaaaaaatc tttgtccaag ccatgcagga atttctagaa aaataccatg ggaaatcaaa
     6901 aggtgtcagc tcttatcttc attccttctg gagctcagcc atcgtctctg tccttctaaa
     6961 atggatcaag gatggcatga aggtaccagc tgaaaagatt gcagatttag gtttaccatt
     7021 ttttaaaaaa tagagaaaaa ggagaaaaga gatgactgaa aaaagactgg cctgggatga
     7081 gtattttgca gcccaagccc tactaattgc gaatcgttcc acttgtaaac gtgctaaagt
     7141 gggcgcgatt ctggtaaaag ataataaggt tatttccact ggttacaatg gttcggtgtc
     7201 agggactgag cattgtattg atcacgaatg tttggtcatt gaaggccact gtgttcgcac
     7261 ccttcacgct gaggtcaatg ctatccttca aggtgctgag cgtggtgttc ccaaaggctt
     7321 tacagcctat gtaacccatt ttccttgtct caactgtaca aaacaattgc ttcaggtcgg
     7381 ctgcaagcgc gtggtttata tcaaccagta ccgaatggac gactacgccc aataccttta
     7441 tcaagaaaaa gggacagaat tgacccattt accacttgag acagtacaga cagctcttaa
     7501 agaggctgat ctaatgtaaa aattatcaaa aataaatggt ttagaaagat ttttaaaccg
     7561 ttttttggta taatgagaag aataaattga aagaaggaat tccaaaaatg ggaaaaattg
     7621 aagttattaa tcacccactg attcaacaca aattgtcaat cttgcgtcgt acagatactt
     7681 ctacaaaagc ttttcgtgag ctagtagatg agattgcaat gttgatgggg tatgaagtac
     7741 ttcgtgatct tccactagaa gatgtggaaa tcgaaacacc aattacaaaa acagttcaaa
     7801 aacaattggc aggtaagaaa ttggccatcg tcccaatctt gcgtgcaggt atcgggatgg
     7861 ttgatggtct cttgagcttg gttccagctg ctaaagttgg ccacatcggt atgtaccgtg
     7921 atgaagaaac acttcaacca gttgaatact tggtgaaatt gcctgaggac attgaccaac
     7981 gtcaaatttt tgtagtagac ccaatgttgg caacaggtgg ctcagcaatc ttggctgttg
     8041 attctcttaa aaaacgtggc gcatcaaata tcaaatttgt ctgccttgta tctgctccag
     8101 agggtgtaaa agcccttcaa gaagctcatc cagatgtaga aatctttaca gcagccttgg
     8161 atgaacgttt gaacgaacac ggttatatcg ttccaggtct tggagatgct ggagaccgct
     8221 tgttcggtac aaaataagat cgaaaagaag aatgacttgg aaaagtattt ccagtcacga
     8281 aaggaggttg ggtttttgtt tctgtctaat gaaagcagag caaaaatttg accttttttg
     8341 accaagatat tataatagtc ttatcttcag tcatttgacc aacaaaatta aaactcaaaa
     8401 ggagaaatga atgattcctg tagttattga acaaacaagc cgtggagaac gttcttacga
     8461 tatttactca cgtcttctca aagaccgcat cattatgctg acaggtccgg ttgaagacaa
     8521 tatggctaac tctgttattg cccaattgct tttcttggat gcccaagata gtacaaaaga
     8581 tatttacctt tatgtcaata caccaggtgg ttctgtttca gctggtttgg caatcgtaga
     8641 taccatgaac tttatcaagg cagatgtcca aaccattgtt atgggaatgg ctgcatctat
     8701 gggaacagtc atcgcatcaa gtggagcaaa aggcaaacgt ttcatgcttc caaatgctga
     8761 atacatgatt catcaaccaa tgggcggtac aggtggtggt acccaacaaa ctgatatggc
     8821 tatcgctgca gaacacttgc tcaaaactcg taataccttg gaaaaaatct tggctgaaaa
     8881 ttcaggtcag tcaatggaaa aagtccatgc agatgcagaa cgtgataact ggatgagcgc
     8941 ccaggaaaca cttgaatatg gctttattga tgaaattatg gccaacaatt cattgaacta
     9001 atgatgatag aaggcaaact cgactgggtt tgcttttttt ggtataatag ggagagattt
     9061 cttagaaaga ggatttatca tgtttgaaaa agtcaatcgc tctggcttga ttatctatct
     9121 ttactataat cgtgatgcca aaaaactgca ggattatgga gatattacct atcattccaa
     9181 gaaacatcgt tacttacaac tctatgttcc aactcaagaa gtggagcaat tggtcggacg
     9241 cttgagcaag gaaaaattta ttaaaaaagt tagagtttgt catatccaag agttggaaac
     9301 accctttgtg ggcaatcttt atcgatagga aaacgttatc atcgaaaaag ttcaaggaaa
     9361 acattgacaa ttttctgata attcggtata ttcttaacag actatttaag aaataaggag
     9421 acaaaaaaga tgaagaaaaa atttgcccta tcgtttgtgg cgcttgcaag tgtagcactt
     9481 cttgcagcct gtggagaagt gaagtctgga gcagtcaaca ctgctggtaa ctcagtagag
     9541 gaaaagacaa ttaaaatcgg gtttaacttt gaagaatcag gttctttagc tgcatacgga
     9601 acagctgaac aaaaaggtgc ccaattggct gttgatgaaa tcaatgccgc aggtggtatc
     9661 gatggaaaac aaatcgaagt agtcgataaa gataataagt ctgaaacagc tgaggctgct
     9721 tcagttacaa ctaaccttgt aacccaatct aaagtatcag cagtcgtagg acctgcgaca
     9781 tctggtgcga ctgcagctgc ggtagcgaac gctacaaaag caggtgttcc cttgatctca
     9841 ccaagtgcga ctcaagatgg attgactaaa ggtcaagatt acctctttat tggaactttc
     9901 caagatagct tccaaggaaa aattatctca aactatgttt ctgaaaaatt aaatgctaag
     9961 aaagttgttc tttacactga caatgccagt gactatgcta aagggattgc caaatctttc
    10021 cgcgagtcat acaagggtga aatcgttgca gatgaaactt tcgtagcagg tgacacagac
    10081 ttccaagcag cccttacaaa aatgaaaggg aaagactttg atgctatcgt tgttcctggt
    10141 tactataatg aggctggtaa gattgtaaac caagcgcgtg gcatgggaat tgacaaacca
    10201 atcgttggtg gtgatggatt caacggtgag gagtttgtac aacaagcaac tgctgaaaaa
    10261 gcatcaaaca tctactttat ctcaggcttc tcaactactg tagaagtttc agctaaagct
    10321 aaagccttcc ttgacgctta ccgtgctaag tacaatgaag agccttcaac atttgcagcc
    10381 ttggcttatg attcagttca ccttgtagca aacgcagcaa aaggtgctaa aaattcaggt
    10441 gaaatcaagg atagccttgc taaaacaaaa gattttgaag gtgtaactgg tcaaacaagc
    10501 ttcgatgcag accacaacac agtcaaaact gcttacatga tgaccatgaa caatggtaaa
    10561 gttgaagcag cagaagttgt aaaaccataa tagaaaaatg ttgaaatagg gaatgagcct
    10621 ttgactcact ccctgtttcg atatttaata ctcttcgaaa atctcttcaa actgcgtcaa
    10681 cgtcgccttg gattatatat gtgactgact tcgtcagtct tatctacaac ctcaaagcag
    10741 tgctttgagc aacctgcggc tagtttccta gtttgctctt tgattttcat tgagtataag
    10801 aacctatcaa aaagtgaggg aaaaccctcg gaattataaa tagaaagagt gaatcttatg
    10861 ctccaacaac tcgtaaatgg tttgattcta ggtagtgttt acgcgctgtt agccctagga
    10921 tataccatgg tttacggaat tatcaagctc atcaacttcg cccatggtga tatttatatg
    10981 atgggagcct ttatcggtta tttcttgatc aattctttcc aaatgaattt ctttgtagcg
    11041 cttattgtag ctatgctagc gacagctatt cttggtgtcg tgattgagtt tcttgcttac
    11101 cgacctttgc gccactctac tcgtattgct gttttgatta cggctattgg ggtttctttc
    11161 ctattggagt atggaatggt ctatctggtt ggtgccaata cccgtgcctt ccctcaagcg
    11221 attcaaacag ttcgatatga tttgggacca attagcttaa caaatgtgca gttaatgatt
    11281 ttggccattt ccttgatttt gatgattttg ttacaagtca ttgtccaaaa gactaagatg
    11341 gggaaagcca tgcgtgcagt atcagtagat agcgacgcgg cgcaattgat ggggatcaat
    11401 gtaaaccgta cgattagctt taccttcgct ttgggttctg ctcttgcggg tgcggctggt
    11461 gttctgattg ctctttatta taactctctt gagcctttga tgggggttac tccaggtctt
    11521 aaatctttcg ttgccgcagt acttggtggt atcggaatta ttcctggtgc ggctcttggt
    11581 ggctttgtga ttggtctatt ggaaaccttt gcgacagcct ttgggatgtc agatttccgt
    11641 gatgccattg tttatggaat cttgttgttg atcttgattg tccgcccagc tggtatcctt
    11701 ggtaagaatg tgaaagagaa ggtgtaaacg atgaaggaaa atttaaaagt taatattcta
    11761 tggttactcc ttttgttagc tggctatagc ttgattagtg tactggtttc agtcggagta
    11821 cttaatctat tctatgtaca gattttacaa caaattggaa ttaatattat tttggctgtt
    11881 ggtctcaact taatcgttgg tttttcagga caattttcac ttggtcatgc tggtttcatg
    11941 gcgattggtg cctatgcagc agctattatt ggttctaaat caccaaccta cggtgccttc
    12001 tttggagcta tgcttgtagg ggctttgctt tcaggagcag ttgccttact tgtcggcatt
    12061 ccaaccttgc gcttgaaggg ggactatctt gcggtagcaa ctctgggtgt ttctgaaatt
    12121 atccgtatct ttatcatcaa tggtggaagt cttacaaatg gtgcggcagg tatcttaggg
    12181 attcctaact ttacaacttg gcaaatggtt tacttctttg tcgtgattac aaccattgca
    12241 accttgaact tcttgcgtag cccaattggc cgttcaaccc tctctgttcg tgaagatgaa
    12301 atcgctgctg agtcagttgg ggttaatacg actaaaatta aaatcatcgc ttttgtcttt
    12361 ggtgccatta ctgcaagtat tgctgggtca cttcaggcag gatttatcgg gtctgttgta
    12421 ccgaaagatt acaccttcat caactcaatc aacgttttga ttattgttgt atttggtgga
    12481 ctcggttcca ttacaggtgc gattgtttcg gctattgttc tgggaatttt gaatatgctt
    12541 ctccaagatg ttgctagtgt gcgtatgatt atttacgctt tggccttggt attggtaatg
    12601 attttcagac caggtggact ccttggaacg tgggaactga gcctatcacg tttctttaaa
    12661 aaatctaaga aggaggaaca aaactaatgg cattacttga agtaaaacag ttaaccaaac
    12721 attttggtgg tctaacagct gttggagatg tgactcttga attgaacgaa ggggaactgg
    12781 ttggattaat cggtccaaac ggagctggga aaaccaccct tttcaacctt ttgaccggtg
    12841 tttatgaacc aagcgaggga acagtaaccc tagatggtca ccttttgaat gggaaatcac
    12901 cttataagat tgcctctttg ggacttggac gtactttcca aaatatccgt ctttttaaag
    12961 atttaacagt tttagacaat gttttgattg cttttggaaa ccatcacaaa cagcatgttt
    13021 ttactagttt cttacgctta ccagcttttt acaagagtga aaaagaatta aaggctaaag
    13081 ctttggaatt gttgaaaatc tttgatttag atggtgatgc agagactctt gctaaaaatc
    13141 tttcctacgg acaacaacgt cgtttggaaa ttgttcgtgc ccttgctacg gaacctaaaa
    13201 ttctcttctt agatgaacca gcagcaggta tgaacccaca ggaaacagcc gaattgactg
    13261 agttaattcg tcgtatcaaa gatgaattta agattacaat catgttgatt gaacacgata
    13321 tgaatctggt catggaagta acagaacgta tctacgtact tgaatatggc cgtttaattg
    13381 ctcaaggaac tccagacgaa attaagacca ataaacgcgt tatcgaagct tatctaggag
    13441 gtgaagccta atgtctatgt taaaagttga aaatctttct gtgcattacg gtatgatcca
    13501 agcagtccgt gatgtaagct ttgaagttaa tgaaggagaa gttgtttccc ttatcggtgc
    13561 caacggtgca ggtaagacaa ctattcttcg caccttgtca ggtttggttc gaccaagttc
    13621 aggaaagatt gaatttttag gtcaagaaat ccaaaaaatg ccagctcaga aaatcgtggc
    13681 aggtggtctt tcacaagttc cagaaggacg ccacgtcttt cctggcttga ctgttatgga
    13741 aaatcttgaa atgggagctt tcttaaagaa aaatcgtgaa gaaaatcaag ctaacttgaa
    13801 gaaggttttc tcacgctttc ctcgtcttga agaacggaag aaccaagatg cagctactct
    13861 ttcaggaggg gaacaacaaa tgcttgccat gggacgcgct cttatgtcaa caccaaaact
    13921 tcttctttta gatgaaccat caatgggact tgccccaatc ttcatccaag agatttttga
    13981 tatcattcaa gatattcaga agcaaggaac aaccgtcctc ttgattgaac aaaatgccaa
    14041 taaagcactc gcaatctctg accgaggata tgtattggaa acaggaaaaa tcgtcctatc
    14101 agggacagga aaagaactcg cttcatcaga agaagtcaga aaagcatatc taggtggcta
    14161 aaacaatcca gtggattgtt ttagtcggca gatgaagatt acgaagtaat catcaatata
    14221 gtccggggga cctttttagc cggtagattg agattgcaaa caaatctgca tctacattga
    14281 aagcttaatt tctaataatt gaaaaaatcg aatgaaaagt ttcttacctt cattcacaga
    14341 gctcgatttt agagctcttt ttgctagctt attcatactt ttctaaattt tgaaaaagaa
    14401 atgtaagcgt ttgatagatt tgcaaaaaga ttgtataata gggataagaa tagaaaagga
    14461 gaagtctcat ggcagttaaa gattttatga cccgcaaggt agtttatatt agtccagata
    14521 caacagtatc tcatgcagca gatttgatga gagagcaagg attgcaccgc ttgcctgtta
    14581 tcgaaaatga tcaattggtt ggtttggtga ctgagggaac cattgcacaa gcaagtccat
    14641 ctaaagcaac aagtctttct atctatgaga tgaattatct tctgaataag acaaaagtaa
    14701 aagatgtcat gattcgcgat gttgtcactg tctcaggcta tgctagtcta gaagatgcaa
    14761 cttatctgat gttgaaaaat aagattggta ttctccctgt cgtagataac catcaagtat
    14821 acggagttat tactgaccgt gacgttttcc aagcctttct tgaaattgca ggttatggcg
    14881 aagaagggat tcgtgtacgc tttgttacag aagatgaagt tggtgttctt ggaaaaatta
    14941 tttctttgat tgtagaagaa aatttgaata tctcccatac agtcaatatt ccgcgtaagg
    15001 atggtaaggt cattatcgaa gtgcaaatcg atggatcaat tgatttacca gccttgaaag
    15061 aaaaatttga agcaaatggt attcaagtgg aagaaatcgc tcgcacttca gcaaaagtct
    15121 tgtaagaagg gaagcccaaa ggcttctttt ttcatgaaaa ggggattaga gcaaaagatg
    15181 gaaagaaatg ataaaatatg ctataatgaa atgatgtaaa aaaggagtat ttatggacat
    15241 ttcagtaatt cgtcagaaaa ttgacgcaaa tcgtgaaaaa ttagcttctt tcagggggtc
    15301 tctttgacct cgaagggcta gaggaagaga ttgccatctt ggaaaacaag atgacagaac
    15361 ctgatttttg gaacgataat attgcggccc aaaaaacgtc gcaagaattg aatgaattaa
    15421 aaaacactta caataccttc cataagatgg aagagttgca ggatgaagtc gaaattttat
    15481 tggatttttt ggctgaagac gagtcagtgc atgatgaact ggtagcgcag ttagccgaac
    15541 ttgataagat aatgaccagc tacgagatga ctctactctt gtcagaacct tatgaccaca
    15601 acaatgccat cttggaaatc catccaggtt ctggtggtac tgaggcgcag gactggggtg
    15661 atatgttgct tcgtatgtat actcgttatg gtaatgctaa aggctttaaa gtggaagtgt
    15721 tggattacca agcaggtgat gaggctggta ttaagtcggt aactttatca tttgaagggc
    15781 ctaatgccta tggtctcctc aagtcagaaa tgggtgttca tcgcttagtg cgaatctcac
    15841 catttgactc tgccaaacgt cgccatacct ctttcacatc tgtagaagtg atgccagaat
    15901 tggatgatac tattgaagtg gaaatccgtg aagatgatat caagatggat accttccgtt
    15961 caggtggtgc cggtggacaa aacgtcaata aggtttcaac aggtgtacgt ttaacccaca
    16021 ttccaactgg aattgttgtc caatcaacag tggatcgtac ccagtatgga aatagagatc
    16081 gcgccatgaa gatgttgcag gctaagctct atcaaatgga gcaagagaag aaggctgcgg
    16141 aggtagattc tctcaaaggt gagaaaaagg agatcacttg gggaagtcaa atccgttctt
    16201 atgtcttcac gccttatact atggtaaaag atcaccgaac tagctatgag gttgctcagg
    16261 tagataaggt tatggatggg gacctagatg gttttatcga tgcttatctc aagtggcgaa
    16321 ttagctaaga tagaaaggaa ctcacatgtc aattattgaa atgagagatg tcgttaaaaa
    16381 atacgacaac ggaacgactg ctctacgcgg tgtttcggtt agcgttcaac cgggggaatt
    16441 tgcttacatc gtaggacctt caggagcagg gaagtcaact tttattcgtt ctctgtatcg
    16501 tgaagtaaaa atcgataaag gaagtctatc agttgctggt tttaatctgg ttaagatcaa
    16561 aaagaaagat gtcccgcttc tacgtcgtag tgttggggtt gtcttccaag attataaact
    16621 gttaccaaag aaaactgtct atgaaaatat tgcttacgct atggaagtaa tcggggaaaa
    16681 tcgccgtaat atcaaaagac gagtgatgga agttttggac ttggttggat tgaagcataa
    16741 ggttcgttct ttcccaaatg aactctcagg tggggagcaa cagcggattg cgattgcgcg
    16801 tgcaattgta aataatccca aagtattgat agctgatgag ccaacaggaa atctggatcc
    16861 ggataattca tgggaaatta tgaatctctt ggaacggatt aacctacaag gaacaactat
    16921 tttgatggcg actcataata gccagattgt aaataccttg cgccaccgtg tcattgccat
    16981 tgaaaatggc cgtgtcgttc gtgacgaatc aaaaggagag tatggatacg atgattagta
    17041 gattttttcg ccatttattt gaagccttaa aaagtttgaa acgaaatggt tggatgacag
    17101 tagctgctgt cagttcagtc atgattactt tgaccttggt ggcaatattt gcatctgtta
    17161 ttttcaatac agcgaaacta gctacagata ttgaaaataa tgtccgtgta gtagtttata
    17221 tccgaaagga tgtggaagat aatagtcaga caattgaaaa agaaggtcaa actgttacaa
    17281 ataatgacta ccacaaggta tatgattctt tgaagaacat gtctacggtt aaaagtgtta
    17341 ccttttcaag taaagaagaa caatatgaaa aattaaccga gataatggga gataactgga
    17401 aaatctttga aggagatgcc aatcctctct atgatgccta tattgtagag gcaaacactc
    17461 caaatgatgt aaaaactata gccgaagatg ctaaaaaaat tgaaggtgtc tctgaggttc
    17521 aagatggcgg tgccaataca gaaagactct tcaagttagc ttcatttatc cgtgtttggg
    17581 gactagggat tgctgctttg ttaattttta tcgcagtttt cttgatttca aataccattc
    17641 gtattaccat tatttcccgc agtcgcgaaa ttcaaatcat gcgcttggtc ggagctaaaa
    17701 acagttatat ccgtggaccg ttcttgttag aaggagcctt tatcggttta ttgggagcta
    17761 tcgcaccatc tgttttggtc tttattgttt atcaaattgt ttaccaatct gtcaacaaat
    17821 cgttggtagg gcaaaatcta tccatgatta gtccagattt atttagtccg ttgatgattg
    17881 ccctactatt tgtgattggg gttttcattg gttcattggg atcaggaata tccatgcgcc
    17941 gattcttgaa gatttaggta aaatagctgc ttttatgagg agattgtaaa atctcctttt
    18001 ttgctacaag agtttttgaa aagagatgcg cagaagaaaa gagcttccaa agaagtcccc
    18061 cagagaagac ttcttgatgg ctaatttgaa cttgaaattt ggcgcactag aaatttttta
    18121 gaaaaaagtg taattttttt ataaaagcct ttacaaaaaa aattaaagtg gtataattaa
    18181 atcatagaaa gtgcaaacgt tttcgtaaaa cgtttgccta aataaaaata aaggagattt
    18241 gaatgatgaa agatacattc aaaaatgtct tgtctttcga attttggcaa aaattcggta
    18301 aggctttgat ggtagttatc gcggttatgc cggctgctgg tttgatgatt tcaatcggta
    18361 agtctatcgt gatgattaac ccaacctttg caccacttgt catcacaggt ggaattcttg
    18421 agcaaatcgg ttggggggtt atcggtaacc ttcacatttt gtttgcccta gccattggag
    18481 gaagctgggc taaagaacgt gctggtggtg ctttcgccgc tggtcttgcc ttcatcttga
    18541 ttaaccgtat cactggtaca atctttggtg tatcaggcga tatgttgaaa aatccagatg
    18601 ctatggtaac tactttcttt ggtggttcaa tcaaagttgc tgattacttt atcagtgttc
    18661 ttgaagctcc agccttgaac atgggggtat tcgtagggat tatctcaggt tttgtagggg
    18721 caactgctta caacaaatac tacaacttcc gtaaacttcc tgatgcactt tcattcttca
    18781 acgggaaacg tttcgtacca tttgtagtta ttcttcgttc agcaatcgct gcaattctac
    18841 ttgctgcttt ctggccagta gttcaaacag gtatcaataa tttcggtatc tggattgcca
    18901 actcacaaga aactgctcca attcttgcac cattcttgta tggtactttg gaacgtttgc
    18961 tcttgccatt tggtcttcac cacatgttga ctatcccaat gaactacaca gctcttggtg
    19021 gtacttatga cattttaact ggtgcagcta aaggtactca agtattcggt caagacccac
    19081 tatggcttgc atgggtaaca gaccttgtaa accttaaagg tactgatgct agtcaatacc
    19141 aacacttgtt agatacagta catccagctc gtttcaaagt tggacaaatg atcggttcat
    19201 tcggtatctt gatgggtgtg attgttgcta tctaccgtaa tgttgatgct gacaagaaac
    19261 ataaatacaa aggtatgatg attgcaacag ctcttgcaac attcttgaca ggggttactg
    19321 aaccaatcga atacatgttc atgttcatcg caacacctat gtatcttgtt tactcacttg
    19381 ttcaaggtgc tgccttcgct atggctgacg tcgtaaacct acgtatgcac tcattcggtt
    19441 caatcgagtt cttgactcgt acacctattg caatcagtgc tggtattggt atggatatcg
    19501 ttaacttcgt ttgggtaact gttctctttg ctgtaatcat gtactttatc gcaaacttca
    19561 tgattcaaaa attcaactac gcaactccag ggcgcaacgg aaactacgaa actgctgaag
    19621 gttcagaaga aaccagcagc gaagtgaaag ttgcagcagg ctctcaagct gtaaacatta
    19681 tcaaccttct tggtggacgt gtaaacatcg ttgatgttga tgcatgtatg actcgtcttc
    19741 gtgtaactgt taaagatgca gataaagtag gaaatgcaga gcaatggaaa gcagaaggag
    19801 ctatgggtct tgtgatgaaa ggacaagggg ttcaagctat ctacggtcca aaagctgaca
    19861 ttttgaaatc tgatatccaa gatatccttg attcaggtga aatcattcct gaaactcttc
    19921 caagccaaat gactgaagta caacaaaaca ctgttcactt caaagatctt actgaggaag
    19981 tttactcagt agcagacggt caagttgttg ctttggaaca agtaaaggat ccagtatttg
    20041 ctcaaaaaat gatgggtgat ggatttgcag tagaacctgc aaatggaaac attgtatctc
    20101 cagtttcagg tactgtatca agcatcttcc caacaaaaca tgcttttggt attgtgacgg
    20161 aagcaggtct tgaagtattg gttcacattg gtttggacac agtaagtctt gaaggtaaac
    20221 catttacagt tcatgttgct gaaggacaaa aagttgcagc aggagatctc cttgtcacag
    20281 ctgacttgaa tgctatccgt gcagcaggac gtgaaacttc aacagtagtt gtcttcacaa
    20341 atggtgatgc aattaaatca gttaaattag aaaaaacagg ttctcttgca gctaaaacag
    20401 cagttgctaa agtagaattg taatatactt gaggttggaa gctgtattcc aacctcttat
    20461 tttgggagaa aagaatgaaa tttttaacac tcaatactca tagttggatg gagaaagaag
    20521 cagaggaaaa attccagatt ttgcttgaag atattcttga aaaggactat gatttgattt
    20581 gtttccaaga aattaatcag gagatgacct cgtcagaggt ggaggttaat gacctttatc
    20641 aagctttgcc agcagctgag cctattcacc aagatcacta tgttagactc ttggttgaaa
    20701 agttatctga gcaagggaaa aattactact ggacctgggc ctataaccat atcggctatg
    20761 accgctacca cgaaggtgtg gctatcttgt ctaaaacacc tattgaagcc agagaaattt
    20821 tggtttcaga tgtggatgat ccaacagact atcatactcg ccgtgttgcc ctagctgaaa
    20881 ctgtagtcga tggcaaggag ctagcagttg ccagtgttca tctctcttgg tgggataaag
    20941 gttttcaaga agaatgggca cgatttgagg ctgtcttgaa aaaattgaac aagccacttt
    21001 tactagctgg agattttaac aacccagcag gtcaggaagg ttaccaagct attttagcta
    21061 gtccattagg cttacaagac gcatttgaag ttgctcaaga gaaaagtggt agctatactg
    21121 ttccgcctga aattgatggc tggaaaggga acactgaacc ccttcgaatc gattatgtct
    21181 ttactaccaa agagttagcg gtggaaaatt tacatgtcgt atttgatggt aacaagagtc
    21241 cacaagtgag tgatcactat ggcttgaatg ctatgttaaa ctggaaataa taactgaaaa
    21301 gaggttggaa ct
//
LOCUS       DAVLRT010000029        20788 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLRT010000029 DAVLRT010000000
VERSION     DAVLRT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.029"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            409..647
                     /locus_tag="VXM85_001068"
                     /pseudo
     CDS             409..647
                     /locus_tag="VXM85_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            791..2020
                     /locus_tag="VXM85_001069"
     CDS             791..2020
                     /locus_tag="VXM85_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1345662.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2040..4736
                     /gene="ppc"
                     /locus_tag="VXM85_001070"
     CDS             2040..4736
                     /gene="ppc"
                     /locus_tag="VXM85_001070"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1345663.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    4819..5036
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5149..6153
                     /locus_tag="VXM85_001071"
     CDS             5149..6153
                     /locus_tag="VXM85_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1345664.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6222..6922
                     /locus_tag="VXM85_001072"
                     /pseudo
     CDS             6222..6922
                     /locus_tag="VXM85_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            6919..7677
                     /locus_tag="VXM85_001073"
     CDS             6919..7677
                     /locus_tag="VXM85_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345665.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            7767..9527
                     /gene="dnaG"
                     /locus_tag="VXM85_001074"
     CDS             7767..9527
                     /gene="dnaG"
                     /locus_tag="VXM85_001074"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1345666.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAAQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            9530..10639
                     /gene="rpoD"
                     /locus_tag="VXM85_001075"
     CDS             9530..10639
                     /gene="rpoD"
                     /locus_tag="VXM85_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1345667.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            10653..10982
                     /locus_tag="VXM85_001076"
     CDS             10653..10982
                     /locus_tag="VXM85_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1345668.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11097..12140
                     /gene="cpoA"
                     /locus_tag="VXM85_001077"
     CDS             11097..12140
                     /gene="cpoA"
                     /locus_tag="VXM85_001077"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1345669.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12156..13481
                     /locus_tag="VXM85_001078"
     CDS             12156..13481
                     /locus_tag="VXM85_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1345670.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            13512..13688
                     /locus_tag="VXM85_001079"
     CDS             13512..13688
                     /locus_tag="VXM85_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1345671.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            13747..13881
                     /locus_tag="VXM85_001080"
     CDS             13747..13881
                     /locus_tag="VXM85_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1345672.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            13945..15255
                     /gene="obgE"
                     /locus_tag="VXM85_001081"
     CDS             13945..15255
                     /gene="obgE"
                     /locus_tag="VXM85_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1345673.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15265..15423
                     /locus_tag="VXM85_001082"
     CDS             15265..15423
                     /locus_tag="VXM85_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345674.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            16473..17732
                     /locus_tag="VXM85_001083"
     CDS             16473..17732
                     /locus_tag="VXM85_001083"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1345675.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            17748..18299
                     /locus_tag="VXM85_001084"
     CDS             17748..18299
                     /locus_tag="VXM85_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1345676.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            18292..19569
                     /locus_tag="VXM85_001085"
     CDS             18292..19569
                     /locus_tag="VXM85_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1345677.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            19585..20445
                     /locus_tag="VXM85_001086"
     CDS             19585..20445
                     /locus_tag="VXM85_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1345678.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         6362 a         3577 c         4677 g         6172 t
ORIGIN      
        1 gaataacttt tccgagagat attaaaacca tcaataaaat agagcgatac tttatatgcc
       61 atgatacaaa tgatatacaa tgatacttct gaccgttcag gctgccatcg taaaagagca
      121 gcaagtgaaa ttcttatgat gacttcatca gtcatgccac gttaaatgtg tgagtttgtt
      181 agagaaacgc aattaatcct caaaaggttc cccgaacctt ttgagttcca cagacgcatc
      241 acgtggagtg tgtaagcttg ttgctaaaag cgtaaaaacc ttggaacgaa aggaataata
      301 gacttcctgc gaaacaaaaa tacaatacaa taaaactatg gatgatgaag caagtaaaca
      361 attaagcgat agccgtttca agagccttgt aggtgttcag cgcacgagat gttagctgtg
      421 ttaaaaacag cttatcaacg caacgtaaac gcgcaaaagg tggacgaaaa accaaattaa
      481 gcctagacga tctcctcatg gtaactattc aatacatgcg agaatagcac acttatgaac
      541 aaattgcggc tgattttggc attcacgaaa gcaacataat tagtcggagt caatggattg
      601 aagcaactct tattcaaaat ggttttacga tttcaaattc tgccttaatt ctgtaaaaac
      661 agtaaaattc gaaggattgt aaggtaagag tttttttctt tctgaaaaaa tggtataata
      721 gcaatcaaaa ctagaaaata aaacggaatt tggaacagat ttgtctgtat cctagtagag
      781 tggtgatact atgaagatta gtaagaggca cttattaaat tattccatct tgattcccta
      841 cttgctttta tctattttgg gcttgattgt ggtctattcg accaccagtg ctattttaat
      901 tgaagaaggc aagagcgcct tgcagttggt tcgaaaccaa ggaatctttt ggattgttag
      961 tttgatactg attgccttaa tttataaatt gagactagat tttttgagaa atgagcgact
     1021 aatcatttta gttatattaa tagaaatgct tttattgttc ttggctcgtt ttattggtat
     1081 ttccgtaaac ggggcatacg gttggatttc ggttgcagga ataactattc agccagctga
     1141 gtacttaaaa atcattatta tttggtattt agctcaccga ttctccaaac agcaagaaga
     1201 aatagctact tatgattttc aagttttgac tcaaaatcaa tggcttcccc gtgcttttaa
     1261 tgattggcga ttcgttctcc tagttctgat tggaagtttg ggaattttcc ctgatttagg
     1321 aaatgcgact attttagtct tggtttcctt gattatgtat acagttagtg gaatcgctta
     1381 tcgctggttt tcaaccattc tggcgctcgt atctgccgct tctgtctttg tcttgaccac
     1441 tatcagccta atcggtgttg agaccttttc aaaaattcca gtatttggct atgtagccaa
     1501 gcgctttagt gcctttttta atccttttgc cgatcgtgct gatgcaggtc accagttagc
     1561 taattcttat tttgccatgg tcaatggcgg ttggtttggt ctaggtcttg gaaactcgat
     1621 tgaaaaacga ggttatttgc cagaagctca tacagacttt gtcttttcta tcgtgattga
     1681 agaatttggc tttgttggtg ccagtcttat tttagctctc ttatttttca tgattttgcg
     1741 gattatcttg gtcggtatcc gagcggagaa tcctttcaat gccatggttg cactcggtgt
     1801 cggagggatg atgttggttc aggtatttgt caatatcgga gggatttcgg gcttgattcc
     1861 atctacagga gtaaccttcc ccttcttatc ccagggtgga aatagtcttc tagtcttatc
     1921 agtggcagta gcctttgtct taaatattga tgccagtgaa aaacgcgcta aattgtaccg
     1981 agaattggaa aatcaaccaa tgaaccttct gttgaagtag gataaagaaa ggatagttta
     2041 tgtctcttca aaaattagaa aattatagta ataaaagtgt tgtgcaagaa gaagtcttga
     2101 ttctaacaga attactggaa gatattacta aaaatatgct tgccccagag acctttgaaa
     2161 aaataataca gttgaaagaa ttatcaacgc aggaagatta tcaaggtcta aaccgtctag
     2221 tgactagctt atcaaatgat gaaatggtct atatttcacg ctatttctct atcttgcctc
     2281 ttttgattaa tatttcagag gatgtggatt tagcttatga aatcaatcat caaaataata
     2341 ttgatcagga ctatttaggt aaattatcta caacgattaa attggtagca gaaaaggaaa
     2401 atgccgttga gatcctagaa cacttgaatg ttgtccctgt tttgacagcc catccaacac
     2461 aagtgcaacg caaaagtatg ttggatttaa caaatcatat tcatagtctt ttgcgtaaat
     2521 accgtgatgt taagttgggg ttgattaata aagataaatg gtacaatgat ttgcgtcgtt
     2581 acatcgaaat tatcatgcag acagacatga ttcgtgagaa aaaattaaaa gtgactaacg
     2641 aaatcacgaa tgctatggaa tattataaca gctccttttt gaaagctgta cctcatttga
     2701 cgacggagta taagcgctta gcgcaagcgc atggtctgaa tttaaaacag gctaaaccaa
     2761 tcaccatggg tatgtggata ggtggtgacc gtgatggaaa tccatttgtt acagcaaaga
     2821 ccttgaagca gtctgcactc actcagtgtg aagtcatcat gaactactat gataaaaaga
     2881 tttaccaact ttatcgtgaa ttttctcttt caactagcat tgtcaacgtc agcaagcaag
     2941 tcagagaaat ggctcgtcaa tccaaggata actcgattta ccgcgaaaaa gagctttacc
     3001 gtcgtgcctt gtttgatatt caatcaaaaa ttcaggcaac taaaacctat ctgattgagg
     3061 atgaagaagt tgggactcgt tatgaaaccg ccaatgattt ctacaaggat ttgattgcca
     3121 ttcgagattc tctactagaa aataagggtg agtccttgat ttcaggtgat tttgtggaat
     3181 tattgcaggc agtagagata tttggttttt acttagcgtc aattgatatg cgacaagact
     3241 ctagcgtcta tgaagcctgt gtggcagaac tcttgaaatc agcaggaatt cattctcgtt
     3301 atagcgagtt gagcgaagaa gaaaagtgtg accttctctt gaaagaatta gaagaagatc
     3361 cccgaattct ttctgcgact cacgcagaaa aatcagaatt attagcaaaa gaattagcta
     3421 tttttaagac ggctcgtgtt ttgaaagata agttgggaga tgatgtcatc cgtcagacca
     3481 tcatttcaca tgcaaccagc ctttctgata tgctagaatt agctattctg ttaaaagaag
     3541 taggactggt ggatacggaa agggcgcgtg ttcagattgt tccccttttt gaaacaattg
     3601 aagacttgga tcattcagag gaaacaatga gaaaatatct ttctcttagc cttgccaaaa
     3661 aatggattga ctcacgaaat aactaccaag aaatcatgct tggctactct gacagtaata
     3721 aagatggcgg ttacctgtca tcatgttgga ccctctacaa ggctcaacaa caattgactg
     3781 ctattggaga tgaatttggc gttaaggtta ccttcttcca tggtcgtggt ggtactgtcg
     3841 gtcgtggtgg tgggccaacc tatgaagcca ttacatctca accgctcaag tctatcaagg
     3901 atcgtatccg cttgacggag cagggtgaag taattgggaa taaatacggt aacaaagacg
     3961 ccgcttacta taaccttgaa atgctagtat cggcagctat taaccgtatg attactcaga
     4021 agaagagcga taccaatacc ccaaatcgtt atgaagccat tatggatcaa gtagtggacc
     4081 gtagttacga tatctaccgt gatttggtct ttggtaatga gcatttctat gattatttct
     4141 tcgagtcaag tccaatcaag gctatttcaa gttttaatat tggttctcgt ccagccgctc
     4201 gtaagactat tactgaaatc ggtggtttgc gtgccatccc ttgggtattc tcatggtcac
     4261 agagtcgtgt tatgttccct ggatggtacg gggttggttc aagcttcaag gaatttatca
     4321 ataaaaatcc agagaatatt gctatcttac gagatatgta ccaaaattgg cctttcttcc
     4381 aatcgcttct ttcaaatgtt gatatggttt tgtcaaaatc aaatatgaat attgcttttg
     4441 aatatgctaa actttgtgaa gacgagcaag ttaaggccat ctatgagact attttaaatg
     4501 aatggcaagt tactaagaac gttatcttgg ctattgaagg acatgacgaa ctcttagctg
     4561 acaatccata tctaaaagct agtctggatt accgtatgcc ttactttaat attctcaact
     4621 atattcagtt agagttgatt aaacgccaac gtcgtggaga attgtccagt gatcaagaac
     4681 gattgattca tatcaccatc aacggaattg cgacaggatt gcgtaattca ggttgataat
     4741 tttcaagagt gaatgctaaa agtgaatatc aaaaaaattc taatagacta ttgacaagta
     4801 gtttaaaaat gatataattt aaccattcag aaaagtaatc atacaaactt tttagagagt
     4861 ctgtggtagc tgaaaacaga taagtggcga tgatgaaaat tgggctgaat gctatttaga
     4921 atttgaaatt ataaaaattc ggtaagcaca ccttacagtg catctcgtta ttgcgagact
     4981 gagcgatagg gaaattccct ataattgagg tggtaccgcg catcgacgtc ctcacacaag
     5041 ttttttgtgt gaggattttt ttgatggagg ttagtatgga aagaaaacga tggcgtcgct
     5101 tgtttagata agtgaaatat gttaaaggaa ataaaaagga gaaacagaat gaaaaataaa
     5161 cgtttaattg gaattattgc tgcattagca gtcttagtag caggaagctt gatttattct
     5221 tcaatgaata aatcagaagc tcagaataat aaggatgaga agaaaataac caagattggt
     5281 gtgcttcaat ttgtgagcca tccatccctt gatttgattt ataaagggat ccaagatgga
     5341 cttgcagaag aaggatataa agatgatcaa gttaaaattg attttatgaa ctcagaaggt
     5401 gatcaaagta aggttgcgac aatgagtaaa caattggttg caaatgggaa tgaccttgtg
     5461 gttggtattg caacaccagc agcccaaggg ttggctagtg caacaaaaga cctaccagtt
     5521 atcatggccg ctattacaga cccaattggt gctaacttgg ttaaagattt gaaaaaacca
     5581 ggtggtaacg ttacaggggt atctgaccac aatccagctc aacaacaaat tgaactcatc
     5641 aaggctctga caccgaatgt gaaaacaatc ggagctcttt actcaagtag cgaagacaat
     5701 tcaaaaacac aggttgaaga atttaaggct tatgctgaaa aagcaggtct gacagtggaa
     5761 acatttgcag ttccttcaac aaatgaaatt gcctcaactg tcactgttat gactatcaag
     5821 gtagatgcta tttgggttcc aattgataac accattgcat caggatttcc aacggttgtc
     5881 tctagcaatc aaagttctaa gaaaccaatt tatcccagtg cgacagctat ggtagaagta
     5941 ggtggtttgg catcagttgt aattgaccaa catgaccttg gtgtggcaac aggtaaaatg
     6001 attgtgcaag tcttgaaagg tgcaaaacca gccgataccc cagtcaatgt cttttcaact
     6061 ggtaagtcag tcatcaataa aaaaatagca caagaactag gtattactat tcctgagtct
     6121 gttctcaaag aagcaggaca agtcatcgaa taaataaggt agggaggagg agactcttcc
     6181 cattttttaa aaagatgaat atagaaagga ttaaataaaa tatgattgtt tccattattt
     6241 ctcaaggatt tgtctgggct attctaggtc tgggaatctt tatgacattt aggattttaa
     6301 actttccaga tatgacgaca gaaggttcct tccctcttgg gggagctgtt gctgtcactt
     6361 tgataaccaa aggcgtgaac ccatttttag cgacacttgt tgctgtagga gcaggttgtt
     6421 tggctggaat ggcagcaggc cttctttata caaaagggaa gatcccaacc ttgctctcat
     6481 gctctttttc ttggacacta aactcggaca agcctatatt gctacagggg ataatcctga
     6541 tatggctaga agtttcggga ttcatactgg acgcatggag ctcatgggct tggtcttatc
     6601 aaatggtgtg attgcccttg caggtgccct cattgctcag caagaaggct atgccgatgt
     6661 gtctcgaggg atcggggtta tcgttgtggg gcttgcaagt ttgattattg gagaagttat
     6721 tttcaagagt ttgagcttgg cagagcgttt ggttactatc gttgtaggtt ctatcgctta
     6781 tcaattttta gtgtgggcag ttatcgcact tggctttaat acaagttacc ttcgtttata
     6841 cagtgccttg attttagcag tctgcctcat gattccaaca tttaagcaaa caatcttgaa
     6901 aggagccaag ttaagcaaat gacagcaatt gtagaattaa aaaatgcaac caaaattgtt
     6961 aaaaatggct ttgatgaaga aaagattatt ttaaatgatg tttccttaga aatttttgaa
     7021 cgggacttta tcacgatttt gggcggaaat ggtgctggaa aatcaaccct ctttaacact
     7081 atagcaggga ccttatcact aactagtgga actatccgta ttttaggtga agatctcact
     7141 aagttttcac ccgagaagcg tgccaagtac ctgtctcgtg tcttccaaga tccaaagatg
     7201 gggacagctc cccgtatgac ggtcgctgaa aatcttttaa tcgccaagtt tcgtggtgag
     7261 aagcgtggat tgttaccacg acgcttgact agctataagg atgaatttca ggcaaccatt
     7321 gaaaaagtag gaaatggtct tgagaaacac ttgaatacac cgattgagtt cttatcaggt
     7381 ggacaaagac aggctttgag tctcttgatg gcaaccttga agcgacctga attactcctg
     7441 ttagatgagc atactgctgc cctggatcca aagactagtg ttgctttgat ggaattgaca
     7501 gatgaatttg ttaagaaaga tcagctaaca gcccttatga ttactcatca tatggaagat
     7561 gctctcaaat acggcaatcg cttgattgtc atgaaagaag gacggattat ccaagattta
     7621 aaccaagaag aaaaagcaaa aatgaaaatc tctgattatt atcaactctt tgaataaagt
     7681 agggaaaatg agtgaaatgg tttacttttt ttctgaaata aagtatacta tataaagtaa
     7741 actatgataa catggaggta ttgtgtatgg ttgacaaaca agtcattgaa gaaatcaaaa
     7801 acaatgccaa cattgtggaa gtcataggag atgtgatttc tttacaaaag gcaggacgga
     7861 actatctagg gctctgtcct tttcatggtg aaaaaacacc ttctttcaac gttgtagagg
     7921 acaagcagtt ttaccactgt tttggttgtg gtcgctcagg tgatgtcttt aaattcatcg
     7981 aggagtacca aggggttccc tttatagagg ctgcccaaat cttaggtcag cgtgtcggga
     8041 ttgaggttga aaaaccgctt tatagtgaac agaagccagc ctcgcctcac caagctcttt
     8101 atgatatgca cgaagatgcg gctaaatttt accatgctat tctcatgaca acgactatgg
     8161 gcgaagaggc cagaaattac ctttatcagc ggggtttgac agatgaagtg cttaaacatt
     8221 tttggattgg tttagcacct ccagaacgaa actatctcta tcaacgtttg tctgatcagt
     8281 atcgtgaaga ggatttactg gattcaggcc tgttttatct ctcggatgcc aatcaatttg
     8341 tagacacctt tcacaatcgc attatgtttc ccctgacaaa tgaccaagga aaggtcattg
     8401 ccttctcagg tcgtatctgg caaaaaacgg attcacaaac ttctaagtat aaaaacagcc
     8461 gatcgactgc aatttttaac aaaagttacg aattatatca tatggatagg gcaaaaaaat
     8521 cttctggaaa aactagtgag atttacctga tggaaggatt catggatgtt attgcagcct
     8581 atcgggctgg aatcgaaaat gctgtggcgt cgatgggaac ggccttgagt cgagagcatg
     8641 ttgagcatct gaaaaggtta accaagaaat tggttcttgt ttacgatgga gataaggctg
     8701 ggcaagccgc gatattgaaa gcattggatg aaattggtga tatgcctgtg caaatcgtta
     8761 gcatgcctga taacttggat cctgatgagt atctacaaaa aaatggtcca gaagacttgg
     8821 cctatctatt aacaaaaact cgtattagtc cgattgagtt ctacattcat cagtacaaac
     8881 ctgaaaacag tgaaaatctg caggctcaga ttgagtttat tgaaaaaata gctcccttga
     8941 ttgttcaaga aaagtccatc gctgctcaaa acagctatat tcatatttta gctgacagtc
     9001 tggcgtcctt tgattatacc cagattgagc agattgttaa tgagagtcgt caggtgcaaa
     9061 ggcagaatcg catggaaaga atttccagac cgacgccaat caccatgcct gtcaccaagc
     9121 agttatcggc tattatgagg gcagaagccc atctactcta tcggatgatg gaatcccctc
     9181 ttgttttgaa cgattaccgt ttgcgagaag actttgcatt tgctacacct gaatttcagg
     9241 tcttatatga cttgcttggc cagtatggaa atcttcctcc agaagtttta gcagagcaga
     9301 cagaggaagt tgaaagagct tggtaccaag ttttagctca ggatttgcct gctgagatat
     9361 cgccgcagga acttagtgaa gtagagatga ctcgaaacaa ggctctcttg aatcaggaca
     9421 atatgagaat caaaaagaag gtgcaggaag ctagccatgt aggagataca gatacagccc
     9481 tagaagaatt ggaacgttta atttcccaaa agagaagaat ggagtaataa tggcaacaaa
     9541 acaaaaagaa gtaacaacat ttgacgtaca ggtagcagaa tttatccgta atcataagca
     9601 aaaagggaca gcaacagatg atgaaatcaa tgcaagtctg gttattcctt ttaccttgga
     9661 cgctgatggg attgaagatc tcttgcaacg gattcaggat gcagggattt ctatcacaga
     9721 taacgaagga aatccaagtg cgcgtgttct tagcaatgaa gaagaaccag aactcagcga
     9781 tgaggacttg attggatcaa cttctgctaa ggtcaatgac cctgtccgta tgtacttgaa
     9841 agaaataggg gtcgttcctc tcttgaccaa tgaagaggag aaagagttgg cactggctgt
     9901 tgaagctggc gatatcgaag ccaaacaacg tcttgcggaa gccaatcttc gtttggttgt
     9961 ttccattgcc aaacgctatg tcggtcgtgg catgcagttc cttgacttga ttcaagaagg
    10021 aaatatgggc ttgatgaagg cggttgacaa gtttgactat tctaaagggt tcaagttttc
    10081 aacttatgca acttggtgga ttcgtcaggc tatcactcgt gctattgcgg accaagctcg
    10141 taccatccgt atcccagttc acatggttga aactatcaat aaattggttc gtgaacagcg
    10201 gaatctcctt caagaattgg ggcaagatcc gacaccagaa cagattgctg aacgaatgga
    10261 tatgacacct gataaggttc gtgaaatctt gaagattgcc caagaaccag tatctcttga
    10321 aactcctatc ggtgaagagg acgatagcca ccttggagac tttatcgaag atgaagtgat
    10381 tgaaaatcca gtggattata cgactcgtat cgtcttgcgt gagcaattgg atgaaatctt
    10441 agatactctt acagaccgtg aagaaaatgt tctgcgtcta cgttttggac tagatgatgg
    10501 aaaaatgcgc acacttgaag atgtggggaa agtctttaac gtaactcgtg agcgtatccg
    10561 tcagattgaa gcaaaggctt tgagaaaact acgccaacca agtcgcagca aaccgcttcg
    10621 tgattttatt gaagactaag agtgaggaaa atatggctta tacagaagag caaattgaaa
    10681 acatcaaaac acggatttta acagccttgg aagaagtcat cgaccctgag ttgggaatcg
    10741 atattgtcaa tcttggtttg atctatgaga ttcgttttga cggtgacaca gggcaaacag
    10801 agattgacat gactttgaca actatgggtt gtcccttagc agaccttttg acagaccaga
    10861 tttatgatgc tatgatagag gttccagaag taacggatac tgaggttaaa ttagtctggt
    10921 atccagcctg gacagttgaa aaaatgagtc gctatgcccg cattgcccta ggcattaagt
    10981 aaacatataa aagcatgtga gagactagag gaaaagcgag gaaatttccc ctcttttcct
    11041 ctagtctctc ctttcttttg ctgattttat tcaaagaaaa tgatataata gtagttatgg
    11101 agaaaaagaa attacgcatc aatatgttga gttcaagtga gaaagtagca ggacagggag
    11161 tttcaggtgc ttaccgtgaa ttagttcgtc ttcttcaccg tgctgccaag gaccaattga
    11221 ttgttacaga aaatcttcca atcgaggcag atgtgactca ctttcatacg attgattttc
    11281 cctattattt atcaaccttc caaaagaaac gctcagggag aaagattggc tatgtgcatt
    11341 tcttgccagc tacacttgaa ggaagtttga aaattccatt tttcttaaag ggaattgtga
    11401 aacgctatgt attttctttt tacaaccgga tggagcactt ggttgtggtc aatcctatgt
    11461 ttattgagga tttggtagca gctggtattc cacgtgaaaa agtgacctat attcctaact
    11521 ttgtcaacaa ggaaaaatgg catcctctac cacaagaaga ggtagtcaga ctgcgcacag
    11581 atcttggtct tagtgacaat cagtttatcg tagtaggtgc tgggcaagtt cagaaacgta
    11641 aagggattga tgactttatc cgtctggctg aggaattgcc tcagattacc tttatctggg
    11701 ctggtggctt ctcttttggt ggtatgacag atggttatga acactataag aaaattatgg
    11761 aaaatccccc taaaaatttg atttttccag gcattgtatc gccagagcgg atgcgcgaat
    11821 tgtatgctct agcggatctt ttcttgttgc ctagttacaa tgagctcttt cctatgacta
    11881 ttttagaagc tgcgagttgt gaggctccta ttatgttgcg tgatttagat ctctataagg
    11941 tgattttgga gggaaattat cgggcgacag cgggtagaga agagatgaaa gaggctattt
    12001 tggaatatca agcaaatcct gctgtcttaa aagatctcaa agaaaaggct aagaatattt
    12061 ccagagagta ttctgaagag catctgttac aaatctggtt ggacttttat gagaaacaag
    12121 ccgctttagg gagaaagtaa aaagtgaggt aatctatgcg aattggttta tttacagata
    12181 cctattttcc tcaggtttct ggtgttgcga ccagtattcg aaccttgaaa acagaacttg
    12241 aaaagcaggg acatgctgtt tttatcttta cgacgacaga taaggatgtc aatcgctacg
    12301 aagattggca aattatccgc attccaagtg ttcctttctt tgcttttaag gatcgtcgct
    12361 ttgcctaccg aggttttagc aaggcacttg aaattgctaa acagtatcag ctagatatta
    12421 tccatactca gacagaattt tctcttggcc tgttggggat ttggattgcg cgtgaattga
    12481 aaattccagt catccatacc tatcacaccc agtatgaaga ctatgtccat tatattgcta
    12541 aggggatatt gatccggccg agtatggtca agtatctggt tagaggtttc ctgcatgatg
    12601 tggatggggt tatttgccct agtgagattg tccgtgactt gctatctgat tataaggtca
    12661 aggttgaaaa acgggtcatt cctactggga ttgaattagc caagtttgag cgtccggaaa
    12721 tcaagcagga aaatttgaaa gaactgcgta gtaaactagg gattcaagat ggtgaaaaga
    12781 cgttgcttag tctttcgaga atctcctatg aaaaaaatat tcaagcagtt ttagcagcct
    12841 ttgctgatgt tctgaaagag gaagacaagg ttaaactggt agtagctggg gatggccctt
    12901 atctgaatga cctcaaagag caagcccaga acctagagat tcaagactca gtcatcttta
    12961 cagggatgat tgctcctagt gagacggctc tttactataa agcggcggat ttcttcattt
    13021 cggcatcgac aagcgaaacg caaggtttga cctacttgga aagcttagcc agtggaacac
    13081 ctgtcattgc tcacggaaat ccttatttga acaacctcat cagtgataaa atgtttggag
    13141 ccttgtacta tggagaacat gatttggctg gtgctatttt ggaagccctg attgcaacac
    13201 cagacatgaa tgagcatacc ttatcagaga aattgtatga gatttcagct gagaactttg
    13261 ggaaacgagt gcatgagttt tatctggatg ccattatttc aaataacttc cagaaagatt
    13321 tggctaaaga tgatacggtc agtcagcgta tctttaggac agttttgtat cttccgcaac
    13381 aggtggttgc tgtacctgta aaaggatcta gacgcatgtt gaaggcttca aaaacacagt
    13441 tgatcagtat gagagactat tggaaagacc atgaagaata gaaagaggaa cagctatgaa
    13501 aaaaacaatt aatgagaagc ggtcgtgata aaaagattgc gggtgtttgt gctggggtgg
    13561 cccattatct ggatatggat ccgactatcg ttcaagtcat ttggggtgtt cttacttgct
    13621 gttacggagc tggaattgta gcttacatta ttttatggat tatcgcacca gtagcaactg
    13681 actattgaaa actagctcaa ttcatgctaa gataatagaa aatagaatgt aacgaaggag
    13741 agaaaaatgg catttggaga taatggaaat cgtaaaaaaa ctatgtttga gaaaataacc
    13801 ttgtttatcg tgattatcat gctagtagca agtttattgg gaatttttgc aactgcaatt
    13861 ggtgccctca gtaatctata aaatagattc aagaaaattt agtgactggg atttcccagc
    13921 ccttttttaa agtgagaaga aataatgagt atgtttttag atacagctaa gattaaggtc
    13981 aaggctggta atggtggcga tggtatggtt gcctttcgtc gtgaaaaata tgtccctaat
    14041 ggaggccctt ggggtggtga tggtggtcgt ggaggcaatg tggtcttcgt tgtagacgaa
    14101 ggactacgta ccttgatgga tttccgctac aatcgtcatt tcaaggctga ttctggtgaa
    14161 aaagggatga ccaaagggat gcatggtcgt ggtgctgagg accttagagt tcgagtacca
    14221 caaggtacga ctgttcgtga tgcggagact ggcaaggttt taacagattt gattgaacat
    14281 gggcaagaat ttatcgttgc ccacggtggt cgtggtggac gtggaaatat tcgtttcgcg
    14341 acaccaaaaa atcctgcacc ggaaatctct gaaaatggag aaccaggtca ggaacgtgag
    14401 ttacaattgg aactaaaaat cttggcagat gtcggtttag taggattccc atctgtaggg
    14461 aagtcaacac ttttaagtgt tattacctca gctaagccta aaattggtgc ctaccacttt
    14521 accactattg taccaaattt aggtatggtt cgcacccaat caggtgaatc ctttgcagta
    14581 gccgacttgc caggtttgat tgaaggggct agtcaaggtg ttggtttggg aactcagttt
    14641 ctccgtcaca tcgagcgtac acgtgttatc cttcacatca ttgatatgtc agctagcgaa
    14701 ggccgtgatc catatgagga ttacctagct atcaataaag agctggagtc ttacaatctt
    14761 cgcctcatgg agcgtccaca gattattgta gctaataaga tggacatgcc tgagagtcag
    14821 gaaaatcttg aagaatttaa gaaaaaattg tctgaaaatt atgatgaatt tgaagagtta
    14881 ccagctatct tcccaatttc tggattgacc aagcaaggtc tggcaacact tttagatgct
    14941 acagctgaat tgttagacaa gacaccagaa tttttgctct acgacgagtc cgatatggaa
    15001 gaagaagctt actatggatt tgacgaagaa gaaaaagcct ttgaaattag tcgtgatgac
    15061 gatgcgacat gggtactttc tggtgaaaaa ctcatgaaac tctttaatat gaccaacttt
    15121 gatcgtgatg aatctgtcat gaaatttgcc cgtcagcttc gtggtatggg ggttgatgaa
    15181 gcccttcgtg cgcgtggagc taaagatggg gatttggtcc gcattggtaa atttgagttt
    15241 gaatttgtag actaggagac tggtatggga gataaaccga tatctttccg agatgcggat
    15301 ggtaattttg tttccgccgc agacgtttgg aatgaaaaga aattggaaga actatttaat
    15361 cgtctcaatc caaatcgtgc cttgagattg gcacgaacta aaaaggaaaa tccatctcag
    15421 taaagaagct aaaaaatccc gtgcctcatc agacacggga ttttgtggta cgacaggcat
    15481 gtatagcaaa ctgaatctgg aatagcacag catatcttct aaaatattgt tataaattga
    15541 tttgaatttc aaaattaaat tgtttgattc ttatctcaat ttgttatagt atatctgatg
    15601 tcaaagttct cggcgagtca aatagcgatt cccaagcctg actatcgtga ggtagcggat
    15661 taaaatggtc tggggataga ccgttttaag tctgacgctg gaaataagaa ttgtcagaag
    15721 aagggatagc gaaatcgtgg ctctacgaac aggaacgtga taataaggcg tatatagcgg
    15781 ataagagggc ctcaaactct aaagtccaaa aaggtagtcg taacctatat gcgtaaatca
    15841 cgagagtaat tgaattcgta ctaagatttt ctattttcac tgtaagcttt taacgtcctt
    15901 atatcttgta tacacgagga aagatgtacg acttatcccg tgaggtctat cactataaag
    15961 agaaaacgac agatagaagt gatcctgagt cacggttatc tttaaaatag attgtctgat
    16021 aggacggtat gtataaaacg cttctgtgaa ctgagagaag ggggagaagt tcttgctaaa
    16081 atttagttga acagccgtat tccgatactt agataagaga tctagtctta gctcctactc
    16141 agttttaggg gataaaaaag gggaaatagc gattcgagaa agattatact cttcgaaaat
    16201 ctcttcaaat cacgtcaata tcgccttgcc gtatgtgtag gatactgact acgtcagttc
    16261 catctacaac ctcaaaacag tgttttgagc aacctgcggc tagtttccta gtttgatctt
    16321 tgattttcat tgagtattag taattcagtt actaactcgt caactctgat ttatccaata
    16381 aaattgaaaa ggatggaaaa aaggataaat ttatgatata ctttattttg aagaccttat
    16441 tagaaatctt gaaagagtat tgaaaactta gaatgagaaa aattgttatc aatggtggat
    16501 taccactgca aggtgaaatc actattagtg gtgctaaaaa tagtgtcgtt gccttaattc
    16561 cagctattat cttggctgat gatgtggtga ctttggattg cgttccagat atttcggatg
    16621 tagccagtct tgtcgaaatc atggaattga tgggagctac tgttaagcgt tatgacgatg
    16681 tattggagat tgacccaaga ggtgttcaaa atattccaat gccttatggt aaaattaaca
    16741 gtcttcgtgc atcttactat ttttatggga gcctcttagg ccgttttggt gaagcgacag
    16801 ttggtctacc gggaggatgt gatcttggtc ctcgtccgat tgacttacac cttaaggcgt
    16861 ttgaagctat gggtgccact gctagctacg agggagataa catgaagtta tctgctaaag
    16921 atacaggact tcatggtgca agtatttaca tggatacggt tagtgtggga gcaacgatta
    16981 atacgatgat tgctgcggtt aaagcaaatg gtcgtactat tattgaaaat gcagcccgtg
    17041 aacctgagat tattgatgta gctactctct tgaataatat gggtgcccat atccgtgggg
    17101 caggaactaa tatcatcatt attgatggtg ttgaaagatt acatgggaca cgtcatcagg
    17161 tgattccaga ccgtattgaa gctggaacat atatatcttt agctgctgca gttggtaaag
    17221 gaattcgtat aaataatgtt ctttacgaac acctggaagg gtttattgct aagttggaag
    17281 aaatgggagt gagaatgact gtatctgagg acagcatttt tgtcgaggaa cagtctaatt
    17341 tgaaagcaat caatattaag acagctcctt acccaggctt tgcaactgat ttgcaacaac
    17401 cgcttacccc tcttttacta agagcgaatg gtcgtggtac aattgtcgat acgatttacg
    17461 aaaaacgtgt aaatcatgtt tttgaactag caaagatgga tgcggatatt tcgacaacaa
    17521 atggtcatat tttgtacacg ggtggacgtg atttacgtgg ggccagtgtt aaagcgaccg
    17581 acttaagagc tggggctgca ctagtcattg ctgggcttat ggctgaaggt aaaactgaaa
    17641 ttaccaatat cgagtttatc ttacgtggtt attctgatat tatcgaaaaa ttacgtaatt
    17701 taggagcgga tattagactt gttgaggatt aaaccgtaga ggtgtttatg aatatttgga
    17761 ccaaattagc aatgttttct ttttttgaaa cggatcgctt gtatttgcgt cctttctttt
    17821 ttagtgatag tcaggacttc cgcgagatag cttcaaatcc agaaaatctt caatttattt
    17881 tcccaacgca ggcaagtctg gaagaaagtc aatatgcact ggccaattac tttatgaagt
    17941 cccctttggg agtgtgggca atttgtgacc agaaaaatca acaaatgatt ggttctatta
    18001 aatttgagaa gttagatgaa atcaaaaaag aagctgagct tggctatttt ttgagaaaag
    18061 atgcttggtc gcaaggattt atgacagagg ttgttagaaa aatttgtcag ctttcttttg
    18121 aggaatttgg cttaaaacaa ttatttatca ttacccacct tgaaaatgaa gctagccaaa
    18181 gagttgctct taagtctgga tttagtttgt tccgtcagtt taagggaagt gatcgttaca
    18241 caagaaaaat gcgggattat cttgaatttc ggtatgtaaa aggagagttc aatgagtaag
    18301 catcaggaaa ttctaagcta tttggaggaa ttaccagtag gtaaaagggt cagtgttcgt
    18361 agcatttcga atcatctagg agttagtgat ggaacagcct atcgagctat taaagaagct
    18421 gaaaaccgtg gaattgtgga gacccgtcct agaagtggaa caattcgtgt taaatcccag
    18481 aaagttgcta tagatagatt aacgtttgct gaaattgcag aagtgacttc ttctgaggtt
    18541 ctggctgggc aagaaggttt agagagagaa tttagtaagt tttcaattgg tgccatgact
    18601 gaacaaaata tcttgtctta ccttcatgat ggggggctct tgattgtcgg agaccgaacc
    18661 cgtattcagt tgctagcctt ggaaaatgaa aatgcagttc tggttacagg gggatttcag
    18721 gttcatgatg atgtgcttaa actggccaat caaaaaggga ttcctgttct aagaagtaag
    18781 catgatacct ttaccgtcgc taccatgatc aataaagcct tgtcaaatgt ccaaatcaag
    18841 actgatattc tgacagttga gaaactttat cgccctagtc atgagtatgg ttttctgaga
    18901 gagacagata cagttaaaga ttatttggac ttggttcgta agaatcgtag cagccgtttc
    18961 cctgttatca atcaacatca ggtcgttgtt ggcgttgtaa ccatgagaga cgctggtgat
    19021 aaatcaccaa gcacgacaat tgataaggtt atgtctcgta gtctattttt ggttggatta
    19081 tcgacaaata ttgccaatgt gagtcaacgg atgatcgcag aagactttga aatggtacca
    19141 gttgttcgaa gcaatcaaac tttgcttggc gttgtgacgc gacgagatgt catggagaag
    19201 atgagccgtt cccaagtttc ggctctacca actttttctg agcagattgg acaaaagctc
    19261 tcttatcacc atgatgaagt agtcattaca gtggaaccct ttatgctaga aaaaaatgga
    19321 gttttggcta atggtgtatt ggcagaaatt ctgacccaca tgacccaaga tttagttgtt
    19381 aatagtggtc gcaatctcat tatcgagcag atgctgatct actttttgca ggctgttcag
    19441 atagatgata tattgcgcat tcaggcacgg attattcatc atacgagacg gtcagctata
    19501 attgattacg atatttatca tggtcaccag attgtttcaa aagcaaatgt gactgttaaa
    19561 attaattaga aactaggaga aaagatgata acattaaaat cagctcgtga aatcgaagct
    19621 atggacaagg ctggtgattt tctagcaagt attcatatag gcttacgtga tttgattaag
    19681 ccaggcgtag atatgtggga agttgaagaa tatgtccgcc gtcgttgtaa agaagaaaat
    19741 ttccttccac ttcagattgg ggttgacggt gccatgatgg actatcctta tgctacctgt
    19801 tgctctctta acgatgaagt ggctcacgct ttccctcgtc attatatctt gaaagatggt
    19861 gatttgctca aagttgatat ggttttggga ggtcccattg ctaaatccga cctgaatgtc
    19921 tcaaaattaa acttcaacaa tgttgaacaa atgaaaaaat acactcagag ctattctggt
    19981 ggtttagcag actcatgttg ggcttatgct gttggtacac cgtccgaaga agtcaaaaac
    20041 ttgatggatg taaccaaaga agctatgtac aagggtattg agcaagctgt tgttggaaat
    20101 cgtatcggtg atatcggtgc ggctattcaa gaatacgctg aaagtcgtgg ttacggtgta
    20161 gtgcgtgatt tggttggtca tggtgttggc ccaactatgc acgaagaacc aatggttcct
    20221 aactatggta ttgcaggtcg tggactccgt cttcgtgaag gaatggtctt aaccattgaa
    20281 ccaatgatca atacaggcga ttgggaaatt gatacagata tgaaaactgg ttgggcgcat
    20341 aagaccattg acggtggatt gtcatgtcag tatgaacacc aatttgtcat tacgaaagat
    20401 ggacctgtta tcttgactag ccaaggtgaa gaaggaactt attaataaaa agtgaaaaga
    20461 ctactggaag tttattttga taaaaaatcc agtagatctt ttcataataa aacgcattgt
    20521 atcaagtgtt aggggctgat atcatgcgtt tttctgcttt taagattttt tccaactttg
    20581 tttgtaagcg catcataaca aagggtctag gattcagggc tctcctccta tatactatta
    20641 gtaaagtaaa actaagggag gatattttag tgtcgcagtc tattgttcct gtagagattc
    20701 cacaatattg tcgttttgat tctaaaaaga gaaatggaat tctgtttaac gtttgtattg
    20761 ccaatcttaa atttattttt ttatatta
//
LOCUS       DAVLRT010000030        20465 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLRT010000030 DAVLRT010000000
VERSION     DAVLRT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20465
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.030"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            111..545
                     /locus_tag="VXM85_001087"
     CDS             111..545
                     /locus_tag="VXM85_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1345679.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAARGGVVNGKELFSTDAEEEEEDFESGI"
     gene            557..1747
                     /locus_tag="VXM85_001088"
     CDS             557..1747
                     /locus_tag="VXM85_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV1345680.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1758..2249
                     /locus_tag="VXM85_001089"
     CDS             1758..2249
                     /locus_tag="VXM85_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1345681.1"
                     /translation="MTMPNIVMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKNVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKVALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2264..3043
                     /locus_tag="VXM85_001090"
     CDS             2264..3043
                     /locus_tag="VXM85_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1345682.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFVFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3030..3848
                     /locus_tag="VXM85_001091"
     CDS             3030..3848
                     /locus_tag="VXM85_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1345683.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEVFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3848..4141
                     /locus_tag="VXM85_001092"
     CDS             3848..4141
                     /locus_tag="VXM85_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1345684.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4163..6064
                     /locus_tag="VXM85_001093"
     CDS             4163..6064
                     /locus_tag="VXM85_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1345685.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPRSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6124..7125
                     /locus_tag="VXM85_001094"
     CDS             6124..7125
                     /locus_tag="VXM85_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1345686.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKKLNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7235..7570)
                     /locus_tag="VXM85_001095"
     CDS             complement(7235..7570)
                     /locus_tag="VXM85_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345687.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7581..7823)
                     /locus_tag="VXM85_001096"
     CDS             complement(7581..7823)
                     /locus_tag="VXM85_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345688.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7839..8033)
                     /locus_tag="VXM85_001097"
     CDS             complement(7839..8033)
                     /locus_tag="VXM85_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1345689.1"
                     /translation="MNRVKEFRKELGISQLELAKNIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            8199..9149
                     /gene="rsmH"
                     /locus_tag="VXM85_001098"
     CDS             8199..9149
                     /gene="rsmH"
                     /locus_tag="VXM85_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1345690.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DRKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            9161..9478
                     /gene="ftsL"
                     /locus_tag="VXM85_001099"
     CDS             9161..9478
                     /gene="ftsL"
                     /locus_tag="VXM85_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1345691.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9482..11734
                     /gene="pbpX"
                     /locus_tag="VXM85_001100"
     CDS             9482..11734
                     /gene="pbpX"
                     /locus_tag="VXM85_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV1345692.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            11736..12716
                     /locus_tag="VXM85_001101"
     CDS             11736..12716
                     /locus_tag="VXM85_001101"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1345693.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLFAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13279..15384
                     /locus_tag="VXM85_001102"
     CDS             13279..15384
                     /locus_tag="VXM85_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1345694.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMSQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFNILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGNFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(15680..16162)
                     /locus_tag="VXM85_001103"
     CDS             complement(15680..16162)
                     /locus_tag="VXM85_001103"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1345695.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(16257..17741)
                     /locus_tag="VXM85_001104"
     CDS             complement(16257..17741)
                     /locus_tag="VXM85_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1345696.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPGGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMSERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            17894..19501
                     /locus_tag="VXM85_001105"
     CDS             17894..19501
                     /locus_tag="VXM85_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1345697.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKELLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            19666..19833
                     /locus_tag="VXM85_001106"
     CDS             19666..19833
                     /locus_tag="VXM85_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV1345698.1"
                     /translation="MKKRILSAGLTFTSDLLLAACGQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(19826..20465)
                     /locus_tag="VXM85_001107"
                     /pseudo
     CDS             complement(19826..20465)
                     /locus_tag="VXM85_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         6177 a         3689 c         4633 g         5966 t
ORIGIN      
        1 taatgaaagc gctaatattt ttttaaaaaa actattgaca agataaaaat aaaggattac
       61 aatggggaat ataaagtaaa ccggtaaacc taaacaagaa aggagaaaag atgaaaattg
      121 tacttgtagg gcatggacat tttgctacag ggatttatag ttctttacaa ttgattgcag
      181 gcaatcaaga aaatgtggag gcaattgact ttgtggaagg aatgtcagca gatgaactca
      241 agcaaaaaat cttacttgca atttcaaatg aagaagaagt tttaatccta agtgatctct
      301 tgggaggatc gccattcaag gtttcttcta ccataatggg agaaaatcca gccaaaacaa
      361 tgaatgttct ctcgggtttg aacttagcca tgttaatgga agcagtcttt gctagaatgg
      421 ctcatagctt tgatgaggtt gttaataaat cagtagtggc ggcccggggc ggagtcgtaa
      481 atggtaaaga attgttttca acggatgcag aggaagagga agaagatttc gaatcgggta
      541 tttaaagggt aaaagaatga taaaaaaggt tacgattgaa aaaataaaat cgcctgagcg
      601 cttcttagaa gtaccacttc tgacgaaaga agaagtcggc caggcaatcg ataaggttat
      661 tcggcagtta gaactcaacc ttgactattt caaggaagat ttcccgacgc cagctacctt
      721 tgataatgtc tatccaatca tggataacac ggaatggacc aatggtttct ggacaggaga
      781 actgtggttg gcttatgaat acagtcaaca ggatgcattt aaaaacatcg ctcataaaaa
      841 tgttctttct ttcctggatc gtgtcaataa gagagtagaa ttggatcacc atgatctcgg
      901 cttcttgtac acaccgtctt gtatggctga atataagata aatggagatg gagaggctag
      961 agaagcaacc ttgaaagctg cagataagtt gattgaacgc tatcaagaaa aaggtggttt
     1021 tattcaagct tggggagact tgggcaagaa agagcattac cgtttgatta tcgactgctt
     1081 gctcaatatc caactcttat tctttgctta tcaagaaaca ggcgatcaaa aatactacga
     1141 tattgcagaa agtcatttct atgcttcagc taataatgta atccgtgatg acgcttcgtc
     1201 cttccacacc ttctattttg atcctgagac aggtcaaccc tttaaaggtg taacgagaca
     1261 agggtatagt gatgattcat gctgggcacg tggtcaatca tggggagtct atggtattcc
     1321 tttgacttat cgtcacttaa aagacgagtc ctgctttgac ttgtttaagg gtgtgaccaa
     1381 ttatttcttg aatcgtctgc caaaagatca tgtgtcctat tgggatttga tttttaatga
     1441 tggtagtgct caatcacgag attcttcagc aacagctatc gccgtctgtg ggattcatga
     1501 aatgctaaaa catctcccag aggtggatgc tgacaaagat atttataaac atgctatgca
     1561 tgccatgctt cgttccttga tcgaacatta tgcaaatgat caatttaccc ctggtgggac
     1621 aagtctcctc cacggtgtgt actcatggca ttcaggtaaa ggagtggatg aaggcaatat
     1681 ctggggtgac tactattacc tagaagccct tatccgtttc tacaaagact ggaacctata
     1741 ttggtaggag gagaaatatg acaatgccaa atattgttat gacccgtatc gatgaacggt
     1801 tgattcatgg acaaggacaa ctttgggtaa aatacctagg ttgtaatacg gtcattgttg
     1861 ccaatgacga agtaagcacg gacaagatgc aacaaactct gatgaaaaca gttgtgccag
     1921 actcagttgc catgcgtttc ttccctttgc aaaaggtgat tgatatcatt cacaaggcta
     1981 atcctgctca aacgatcttt atcgttgtaa agaatgtgaa ggatgcttta accttggtag
     2041 aaggtggtgt ccctatcaaa gaaatcaata ttgggaacat tcacaatgct actggtaaag
     2101 agcaagtgac acgctccatc ttcctgggtg aagaggacaa ggtggccctc aaggaattga
     2161 gccaaactca tcaagtaaca tttaatacga aaacaactcc aacaggaaat gatggagctg
     2221 ttcaagtcaa cattatggac tatatttaaa agaggagatc gttatgtcga ttaatgtatt
     2281 tcaagcgatt ttaattggat tatggacagc tttctgtttt agtggaatgc tgttaggaat
     2341 ttacaccaat agatgtattg ttctgtcatt tggtgtcgga attattctag gtgatctgcc
     2401 tactgctctt gcaatgggag ctattggtga attggcttat atgggattcg gtgttggtgc
     2461 tggaggtact gttccaccaa acccaatcgg acctggtatc tttggtacct tgatggctat
     2521 cactagtgct ggtaaagtca gtccagaagc ggctcttgcc ctctctactc cgattgctgt
     2581 ggcgattcaa ttcttacaaa ctttcgccta cactgtacgt gctggtgcgc ctgaaacggc
     2641 gatgaagcac ttgaaaaacc ataatttgaa gaaattcaag ttcactctaa atgcaacaat
     2701 ttggttgttt gtctttattg gatttacctt gggttgcttg ggcgcccttt caatggatac
     2761 cttgttgaaa ctcgtagact acattccacc ggtattgctt acaggtttga cagttgctgg
     2821 taaaatgctc ccagctatcg gttttgcgat gatcttgtca gtgatggcta agaaagagtt
     2881 gattcccttt gtcttgttgg gatatgtttg tgcagcttat ctaaacatcc caacaattgg
     2941 tattgcaatt gtaggtacta tctttgcttt gattgaattt tataacaagc caaaaacagc
     3001 ggatcatgtg gtagaggagg aagcacacga tgactggatc taacaaatta acaaaacgtg
     3061 attatcttaa aacgtctttg cgggcattct tttgtcaaaa tggatttaac tatagtaact
     3121 atcaagggtt gggatatgcc aatgtgatgt atcctgcttt gaaaaaacac tatggagagg
     3181 atcaggaagt tttctaccaa gccttggaag aaaactgtga attctataat accaacccac
     3241 acttcctgcc ttttattacc agcttgcatc ttgtaatgtt ggaaaatggc cgaccaacaa
     3301 aagaaacacg tagcatcaag atggccttga tgggaccatt ggcaggtatt ggggattctc
     3361 tttctcaatt ctgtttagct ccattgttct caaccatcgc agcttcgttt gctcaagaag
     3421 gcttggttgt cggtccaatc ttgttcttcc ttgcgatgaa tacgatttta acagcgatta
     3481 aattgtcaac tggtctgtat ggatacaaac taggaacaac tgtgattgat aaactaagcg
     3541 aacagatggc aacgatttct cgtattgcca atattatcgg tgtaaccgta attgctggtt
     3601 tggcagcgac atctgttaag attatggtgc cgattacctt tgctgcaggg gaagttaaag
     3661 cagacgctaa acaaagtatc gtaagtattc agggaatgct tgataaggtt gctccagctc
     3721 ttctaccagc cctatttaca cttttagttt actacttgat caaagaaaag aaatggacaa
     3781 catataaact cgttatttta acagttatca tcggaattat cggaagctgg cttaagatta
     3841 tagcttaatg gatacaacat tgttttatgg aatagtgatt gtcttggcag tgagtcccct
     3901 tttactgtca agctttcatt ctattcgtca gcaaaagttg cttcgcaaac agatggagca
     3961 acgacaagag tatttagctt ctttaacatc tggtgatgaa gtgttgttat tgtctggaat
     4021 tcatggaaaa atcatttcta tcaaagatga cttggtctcc ttgcagattg caaaaggtgt
     4081 ggtcatctat gtagaaaagg aaagtgtaat gggaaagaca aaagaactgc tttttaagta
     4141 gttctttttc tggtaataga taatgaaaga agaaagaaga caattttttg aaagaatcga
     4201 tggaaaccaa tgtcgtgatt atatcttgtc ccactgttca aaagactatg agaaagtcaa
     4261 gagttccctt gaacgcttga tggacaatcg ttttatgttt gatagtcctt gggatatgga
     4321 gccttgttca aaaatccatc aaatccagcc gatggtatgg gatcaagtat ttgaagatga
     4381 tccagaatgg gcttatatgc tcaatcgaca agaatatctc ttgcagttta tgatagggta
     4441 tctggtagaa ggagataagg actatattca aaagtgcaag ttctttctat ttgattggat
     4501 tgagcaggtg agagaatttt ctcctcaatc cttgatgact agaaccttgg atacgggtat
     4561 tcgttccttt acttggttga aactactctt gcttctcttg aaatttgact tgctagagga
     4621 gaaagaacta gagaaaattt tggtcagtct agaaaagcag attgacttta tgaaaagcta
     4681 ctatcgcgcc aagtacaccc ttagtaactg ggggatttta caaacaattc cgatgcttgc
     4741 tatctatcat ttcttttcag ataagatgga cctagaagaa gcttaccatt ttgcttcaga
     4801 ggagttgaaa cagcaaattg agacacagat tttaggagat ggaagccagt ttgaacagtc
     4861 gattctctat catgtagagg tttataaagc cttgctggat ttgtgtctct tgcttccaga
     4921 cttgcaagat agttaccaag agttgctgga aaagatggcg acctatattc aaatgatgac
     4981 aggcttagat ggacgaactt tggcttttgg tgatagcgat tctacagaaa cgacagaaat
     5041 tttgagcctg tctgctgtgg ttttgaacaa ggaagacctt cttaacggtc tggatgttaa
     5101 agtcgatttg cttagcctct tgttcctggg gcgagaaaag gtcaagcgac tgcaggaatt
     5161 tgaaaagaga gcttggcagc ctaggtccat gatctttgaa gactctggac atgtctgcat
     5221 taaggatgaa catcgttatc tatttttcaa aaatggtccg ctaggaagtg cccatagcca
     5281 tagtgacgag aatagttttt gcttacagta tcaaggccaa cctattttca tagatgctgg
     5341 gcgttattct tatcgggaga tatatgaacg ttatctctta aagagtgctt ggagtcattc
     5401 gacctgcatt gtagatggga aagctccgga aagaatcacg ggatcctggg aatatgaata
     5461 ctatcctcac tccctgtttt gtcaccataa agaaagggag ggagtgcatt atattgaagg
     5521 ggcttattgg tcagcagaac ctgatttgcc ttatcttcac aagagaaaaa tcctcatgtt
     5581 ggtagaggat gtctggctct tggtagatga catcaggtgt caaggtcagc atgaggcgtt
     5641 gactcagttt atccttgaca aggatgtgac ctatcaagat gggaaaatca atcagttgag
     5701 actatggagt gaagttgatt ttgatttgga agataccatc atttctccta aatacaatga
     5761 gcttgaaaga agtagcaaac tcaccaagcg ccaattcttt gagaatcaga tgctggatta
     5821 taccatcatt gcgcatgaga gttttgaaat catccgtcat tctgtctacc agacagatga
     5881 tcgtgaagtg gaaaatgctc tggcttttga agtgaaaaat gacgaaacag acaagctgat
     5941 tctgttatta agcgaggata ttggtgtagg tgaaaaattg tgcctcgttg acggaacaaa
     6001 aatgcgtgga aaatgtctag tatatgataa aataaatgag agaatgattc gcttgcagtg
     6061 ctagaaatag gcattttgaa tagtgaacat gttataataa gtattagtag gaggtgtttt
     6121 agattggaga agaaactgac cataaaagac attgcggaaa tggctcagac ctcgaaaaca
     6181 accgtgtcat tttacctaaa cgggaaatat gaaaaaatgt cccaagagac acgtgaaaag
     6241 attgaaaaag ttattcatga aacaaattac aaaccgagca ttgttgcgcg tagcttaaac
     6301 tccaaacgaa caaaattaat cggtgttttg atcggtgata ttaccaacag tttctcaaac
     6361 caaattgtta agggaattga ggatatcgcc agccagaatg gctaccaggt aatgatagga
     6421 aatagtaatt acagccaaga gagtgaggac cggtatattg aaagcatgct tctcttggga
     6481 gtagacggct ttattattca gccgacctct aatttccgaa aatattctcg tatcatcgat
     6541 gagaaaaaga agaaaatggt cttttttgat agtcagctct atgaacaccg gactagctgg
     6601 gttaaaacca ataactatga tgccgtttat gacatgaccc agtcctgtat cgaaaaaggt
     6661 tatgaacatt ttctcttgat tacagcggat acgagtcgtt tgagtactcg gattgagcgg
     6721 gcaagtggtt ttgtggatgc tttaacagat gctaatatgc gtcacgccag tctaaccatt
     6781 gaagataagc atacgaattt ggaacaaatt aaggaatttt tacaaaaaga aatcgatccc
     6841 gatgaaaaaa ctctggtatt tatccctaac tgttgggccc tacctctagt ctttaccgtt
     6901 atcaaaaagt tgaattataa cttgccacaa gttgggttga ttggttttga caatacggag
     6961 tggacttgct tttcttctcc aagtgtttcg acgctggttc agccctcctt tgaggaagga
     7021 caacaggcta caaagatttt gattgaccag attgaaggcc gcaatcaaga agaaaggcaa
     7081 caagtcttgg attgtagtgt gaattggaaa gagtcgactt tctaaaatga aggaaaatga
     7141 cttgcaatct ctgttaagaa ataaaataat cccacctaga acaagctagg tgggattatt
     7201 tgcctatgaa atgagaaatt atgggagcaa gctcctaaat caactgtttt tgatctactt
     7261 ctttaactac ttgataaaag ttatagaagt aggccaaact tgaaatgatg gttacgacta
     7321 ggaatattga aaatttccat tggacagggt tggttaaaag ttgtggaaag gatatgagga
     7381 gaaagaagag ggctgcgttg aggacaggta tccgttttga ttgtattttc tcaagtcctt
     7441 tattgagcgc aggaagaaag aggagtagga gtagtaaaac tgtatgagaa acagctcctg
     7501 aaataagggc aaagaaaagg aaaatactga taaaaacatg aatgatcagt agtctagcta
     7561 gtgatttcat aaggtacctc ctaatcctgg tcttttttag ctcttgcaat acgaagtgag
     7621 tcgacaatat gtatcatcac tccgaaaaag aaagctccca gtatagtttt aaaaatatgt
     7681 tttgtattta gaagagaact gataaaattt ggattttcac ttgttagggt atcaatgagt
     7741 ggaattataa aaaatatcac tgttccataa atcgaacctg ctttcagacc aggataacgt
     7801 aactgtttct tttctttttt catgagtttg ctcctttttt aaaaatcttc ctcccaaaag
     7861 agactgttga ggtcagtttg gaggctgcgg gcgagattga gacagagttc caaggttgga
     7921 ttgtacttgt cgttttcaat catattgata gtctgtctcg agacaccgat attcttggcg
     7981 agctcgagct gggaaatgcc cagttccttg cgaaattctt tcacacgatt catctgttct
     8041 tctttctgat ttatgtcgta tatatttgac tatattatag tcttttaaac ataaagtgtc
     8101 aagtattttt gacatatttt ttgaagaaat agtagtctcc ttgtcctatt tgtctgacaa
     8161 gtgcaagctg gtcggatttg tggtaaaata gataagatat gacaaaagaa tttcatcatg
     8221 taacggtctt actccacgaa acgattgata tgcttgacgt aaaacctgac ggtatctacg
     8281 ttgatgcgac tttgggtgga gcaggccata gcgagtattt attaagtaaa ttaagtgaaa
     8341 aaggccatct ctatgccttt gaccaggatc agaatgccat tgacaatgcg caaaaacgct
     8401 tggcacctta cattgagaag ggaatggtga cctttatcaa ggataacttc cgtcatttac
     8461 aggcacgttt gcgcgaagct ggtgttcagg aaattgatgg aatttgttat gacttgggag
     8521 tgtctagtcc tcaattagac cagcgtgagc gtggtttttc ttataaaaag gatgcgccac
     8581 tggacatgcg gatgaatcag gatgctagtc tgacagcgta tgaagtggtg aacaattatg
     8641 actatcatga cttggttcgt attttcttca agtatggaga ggacaaattc tctaaacaga
     8701 ttgcgcgtaa gattgagcaa gcgcgtgaag tgaagccgat tgagacaacg actgagttag
     8761 cagagattat caagttggtc aaacctgcca aggaactcaa gaagaagggt catcctgcta
     8821 agcagatttt ccaggctatt cgaattgaag tcaatgatga actgggggcg gcagatgagt
     8881 ccatccagca ggctatggat atgttggctc tggatggtag aatttcagtg attacctttc
     8941 attccttaga agaccgcttg actaagcaat tgttcaagga agcttcaaca gttgaagttc
     9001 caaaaggctt gcctttcatc ccagatgatc gcaagcccaa gatggaattg gtgtcccgta
     9061 agccaatctt gccaagtgcg gaagagttag aagccaataa ccgctcgcac tcagccaagt
     9121 tgcgcgtggt cagaaaaatt cacaagtaag agggaaaaag atggcagaaa aaatggaaaa
     9181 aacaggtcaa atactacaga tgcaacttaa acggttttcg cgtgtggaaa aagcttttta
     9241 cttttccatt gctgtaacca ctcttattgt agccattagt attattttta tgcagaccaa
     9301 gctcttgcaa gtgcagaatg atttgacaaa aatcaatgcg cagatagagg aaaagaagac
     9361 cgaattggac gatgccaagc aagaggtcaa tgaactatta cgtgcagaac gtttgaaaga
     9421 aattgccaat tcacacgatt tgcaattaaa caatgaaaat attagaatag cggagtaaga
     9481 tatgaagtgg acaaaaagag taatccgtta tgcgaccaaa aatcggaaat cgccggctga
     9541 aaacagacgc agagttggaa aaagtctgag tttattatct gtctttgttt ttgccatttt
     9601 tttagtcaat tttgcggtca ttattgggac aggcactcgc tttggaacag atttagcgaa
     9661 ggaagctaag aaggttcatc aaaccacccg tacagttcct gccaaacgtg ggactattta
     9721 tgaccgaaat ggagtcccga ttgctgagga tgcaacctcc tataatgtct atgcggtcat
     9781 tgatgagaac tataagtcag caacgggtaa gattctttac gtagaaaaaa cacaatttaa
     9841 caaggttgca gaggtctttc ataagtatct ggacatggaa gaatcctatg taagagagca
     9901 actctcgcaa cctaatctca agcaagtttc ctttggagca aagggaaatg ggattaccta
     9961 tgccaatatg atgtctatca aaaaagaatt ggaagctgca gaggtcaagg ggattgattt
    10021 tacaaccagt cccaatcgta gttacccaaa cggacaattt gcttctagtt ttatcggcct
    10081 agctcagctc catgaaaatg aagatggaag caagagcttg ctgggaacct ctggaatgga
    10141 gagttccttg aacagtattc ttgcagggac agacggcatt attacctatg aaaaggatcg
    10201 tctgggtaat attgtacccg gaacagaaca agtttcccaa cgaacgatgg acggtaagga
    10261 tgtttataca accatttcca gccccctcca gtcctttatg gaaacccaga tggatgcttt
    10321 tcaagagaag gtaaaaggaa agtacatgac agcgactttg gtcagtgcta aaacagggga
    10381 aattctggca acaacgcaac gaccgacctt tgatgcagat acaaaagaag gcattacaga
    10441 ggactttgtt tggcgtgata tcctttacca aagtaactat gagccaggtt ccactatgaa
    10501 agtgatgatg ttggctgctg ctattgataa taataccttt ccaggaggag aagtctttaa
    10561 tagtagtgag ttaaaaattg cagatgccac gattcgagat tgggacgtta atgaaggatt
    10621 gactggtggc agaatgatga ctttttctca aggttttgca cactcaagta acgttgggat
    10681 gaccctcctt gagcaaaaga tgggagatgc tacctggctt gattatctta atcgttttaa
    10741 atttggtgtt ccgacccgtt tcggtttgac ggatgagtat gctggtcagc ttcctgcgga
    10801 taatattgtc aacattgcgc aaagctcatt tggacaaggg atttcagtga cccagacgca
    10861 aatgattcgt gcctttacag ctattgctaa tgacggtgtc atgctggagc ctaaatttat
    10921 tagtgccatt tatgatccaa atgatcaaac tgctcggaaa tctcaaaaag aaattgtggg
    10981 aaatcctgtt tctaaagatg cagctagtct aactcggact aacatggttt tggtagggac
    11041 ggatccggtt tatggaacca tgtataacca cagcacaggc aagccaactg taactgttcc
    11101 tgggcaaaat gtagccctca agtctggtac ggctcagatt gctgacgaga aaaatggtgg
    11161 ttatctagtc gggttaaccg actatatttt ctcggctgta tcgatgagtc cggctgaaaa
    11221 tcctgatttt atcttgtatg tgacggtcca acaacctgaa cattattcag gtattcagtt
    11281 gggagaattt gccaatccta tcttggagcg ggcttcagct atgaaagact ctctcaatct
    11341 tcaaacaaca gctaaggctt tggagcaagt aagtcaacaa agtccttatc ctatgcccag
    11401 tgtcaaggat atttcacctg gtgatttagc agaagaattg cgtcgcaatc ttgtacaacc
    11461 catcgttgtg ggaacaggaa cgaagattaa aaacagttct gctgaagaag ggaagaatct
    11521 tgccccgaac cagcaagtcc ttatcttatc tgataaagca gaggaggttc cagatatgta
    11581 tggttggaca aaggagactg ctgagaccct tgctaagtgg ctcaatatag aacttgaatt
    11641 tcaaggctcg ggctctactg tgcagaagca agatgttcgt gctaacacag ctatcaagga
    11701 cattaaaaaa attacattaa ctttaggaga ctaatatgtt tatttccatc agtgctggaa
    11761 ttgtgacatt tttactaact ttagtaggaa ttccggcctt tatccaattt tatagaaagg
    11821 cgcaaattac aggccagcag atgcatgagg atgtcaaaca gcatcaggca aaagctggga
    11881 ctcctacaat gggaggtttg gttttcttga ttacttctgt tttggttgct ttctttttcg
    11941 ccctatttag tagccaattc agcaataatg tgggaatgat tttgttcatc ttggtcttgt
    12001 atggcttggt cggattttta gatgactttc tcaaggtctt tcgtaaaatc aatgaggggc
    12061 ttaatcctaa gcaaaaatta gctcttcagc ttctaggtgg agttatcttc tatcttttct
    12121 atgagcgcgg tggcgatatc ctgtctgtct ttggttatcc agttcatttg ggatttttct
    12181 atattttctt cgctcttttc tggctagtcg gtttttcaaa cgcagtaaac ttgacagacg
    12241 gtgttgacgg tttagctagt atttccgttg tgattagttt gtttgcctat ggagttattg
    12301 cctatgtgca aggtcagatg gatattcttc tagtgattct tgccatgatt ggtggtttgc
    12361 tcggtttctt catctttaac cataagcctg ccaaggtctt tatgggtgat gtgggaagtt
    12421 tggccctagg tgggatgctg gcagctatct ctatggctct ccaccaggaa tggactctct
    12481 tgattatcgg aattgtgtat gtttttgaaa caacttctgt tatgatgcaa gtcagttatt
    12541 tcaaactgac aggtggtaaa cgtattttcc gtatgacgcc tgtacatcac cattttgagc
    12601 ttgggggatt gtctggtaaa ggaaatcctt ggagcgagtg gaaggttgac ttcttctttt
    12661 ggggagttgg gcttctagca agtctcctga ccctcgcaat tttgtatttg atgtaagaat
    12721 ggcaccctga tgtttcaggg tgtttttgtg tttaaataca caatgaaaat caaagaacaa
    12781 actagaaagc taactttagg ctgctcaaaa tataatatat tgaaactaga atagtacacc
    12841 tctacttcta aaacattgtt agaaatcgat ttgactgtcc tgaacgattt atcctgttct
    12901 tatttcattt tactatacag tttcgaggtt gtagataagg cgaagctgat gtggtttgaa
    12961 gagattttcg aagagataaa aaatattggt caattaaagt caaaatcctt gaccttttct
    13021 gactaatgta gtatattatg aacgtaaggt aaatgaaagc cttactagaa cacttattta
    13081 aagtaaaata gaaagaggtg gggtctatgt ttaatctaga aatcttcaga agtaaagatg
    13141 gtctactcct gcttgaaaaa gaaaaaccag aaatagtacg tagggtagcg gtttagctag
    13201 tctaaatttt ttaaaacaaa ggtcaaagat agtcaatatc agtaatcata actaagtaaa
    13261 caaaaagagg taaagaatat gaataacaac tttaataatt ttaataacat ggatgattta
    13321 tttaaccaat tgatgggtgg tatgcgagga tacagttctg aaaatcgccg ttacttaatt
    13381 aatgggcgcg aagtcacacc tgaggaattt gctcactatc gtacgactgg tcaattacca
    13441 ggaaatgcag aaactgatgt gcaaatgtca caacaggcat caggtatgaa acaagacggt
    13501 gtccttgcaa aactaggtcg aaacttgaca gcagaagcgc gtgagggtaa gttggatcct
    13561 gttatcggac gaaacaagga aattcaagaa acatctgaaa tcctctcacg ccgcaccaag
    13621 aacaatcctg ttttggtcgg agatgcaggt gttggtaaga cagcagttgt cgaaggtcta
    13681 gcgcaagcca ttgtgaacgg agatgttcct gctgctatca agaacaagga aattatttct
    13741 attgatatct caggtcttga ggctggtact caataccgtg gtagctttga agaaaatgtc
    13801 caaaacttag tcaatgaagt gaaagaagca gggaatatta tcctcttctt tgatgaaatt
    13861 caccaaattc ttggtgctgg tagcactggt ggagacagtg gttctaaagg acttgcggat
    13921 attctcaagc cagctctctc tcgtggagaa ttgacagtga ttggggcaac aactcaagac
    13981 gaataccgta acaccatctt gaagaatgct gctcttgctc gtcgtttcaa cgaagtgaag
    14041 gtcaatgctc cttcagcaga gaatactttt aacattcttc aaggcattcg tgacctctat
    14101 caacaacatc acaatgtcat cttgccagac gaagtcttga aagcagcggt ggattattct
    14161 gttcaataca ttcctcaacg tagcttgcca gataaggcta ttgaccttgt cgatgtaaca
    14221 gctgctcact tggcggctca acatccagta acagatgtgc atgctgttga acgagaaatc
    14281 gaaacggaaa aagacaagca agaaaaagca gttgaagcag aagattttga agcagctcta
    14341 aactataaaa cacgcattgc agaattggaa aagaaaatcg aaaaccacac agaagatatg
    14401 aaagtgactg caagtgtcaa cgatgtggct gaatctgtgg aacgaatgac aggtatccca
    14461 gtatcgcaaa tgggagcttc agatatcgaa cgtttgaaag atatggctca tcgcttgcaa
    14521 gacaaggtga ttggtcaaga taaggccgta gaagttgtag ctcgtgctat ccgtcgtaac
    14581 cgtgctggtt ttgatgaagg aaatcgccca atcggcaact tcctctttgt agggtctact
    14641 ggggttggta agacggagct tgctaagcaa ttggcactcg atatgtttgg aacccaggat
    14701 gcgattatcc gtttggatat gtctgaatac agtgaccgca cagctgtttc taagctaatt
    14761 ggtacaacag caggctatgt gggttatgat gacaatagca ataccttaac agaacgtgtt
    14821 cgtcgcaatc catactctat cattctcttg gatgaaattg aaaaggctga ccctcaagtt
    14881 attacccttc tcctccaagt tctagatgat ggtcgtttga cagatggtca aggaaataca
    14941 gtaaacttca agaacactgt cattattgcg acctcaaatg ctggatttgg ctatgaagcc
    15001 aacttgacag aagatgcgga taaaccagaa ttgatggacc gtttgaaacc cttcttccgt
    15061 ccagaattcc tcaaccgctt taatgcagtc atcgagttct cacacttgac taaggaagac
    15121 ctttctaaga ttgtagattt gatgttggct gaagttaacc aaaccttggc taagaaagac
    15181 attgacttgg tagtcagtca agcggctaaa gattatatca cagaagaagg ttacgacgaa
    15241 gtcatggggg ttcgtcctct ccgtcgagtg gttgaacaag aaattcgtga taaggtgaca
    15301 gacttccact tagatcattt agatgctaaa catctggaag cagatatgga agatggcgtt
    15361 ttggttattc gtgagaaagc ctaagataga attttgagga taaaaaagaa ggagccagct
    15421 gaaaaaaact ggttcctttt gaggtacgac gggcatgtcg tacagtagaa gtgtactatt
    15481 ctagtttcaa tccactatag tagctcagaa gtcggtactt aaacgtgcta tatcaaaacc
    15541 agtccttgaa aaacgtggat tggtttcgtg tttggattat taccttgaac gacatgcgtt
    15601 aaaagttagt tgaaccgccg tatgccgaac ggcacgtacg gtggtgtgag aggggctaga
    15661 gattatcccc tactcgattt taaatcacat gacgttcaaa ggcatcatct gaaatccctt
    15721 gttccaagat gagttttgcc cattctttag cagagaagag gctgtggtcc ttgtagtttc
    15781 cgcaagattc gatggttgtc cctgggacat cttcccaagt agtagtttca gcgatttcct
    15841 tgagcgaatc cttgataaca gctgcgattt tagcactggt gtgacgtccc cacataatca
    15901 tgtggaagcc tgtgcggcaa ccaaatggtg aacagtcaat catgccgtca atgcgggtac
    15961 ggatgagttt ggctaagagg tgctcgatag tgtgaaggcc ggcagtaggg atagagtctt
    16021 cgtttggttg caccaagcga atatcataat tggagatgat gtctcctttt ggtcctgttt
    16081 cttccccaat caagcgaaca tagggtgctt tgacaatggt gtggtcaagt tcaaaacttt
    16141 cgacaataac ttcttttgac atggtaaatc ctttcagttt tcttctctca ttatatcata
    16201 aaggttgttc ctgagacaga gagaaaacct ctccgaggct agagaggttg aaatctttac
    16261 ttacgatata agcggtcgta ttggtagtat gggtcaaagg ttacgttgat acccagttta
    16321 cgaaggacat tcttgtcttc atcagtcaag atgatggttg agtgggcttc gcttcctttg
    16381 aggttgccta gttcttccat agcgcgggca gcatcaggat tttctgtagc tgtgatagca
    16441 agtgcaatca ggatttcatt tgaatgaagg cgtggattgc ggctaccgag atgatcgatt
    16501 ttaagacctt ggattggctt aacaacttca ggttcgatta gttttacttc tttagcgatg
    16561 tcagctgatt ttttgatggc gttgatcaag gcagcggctg taggaccaaa gagttctgag
    16621 ttcttaccag tgacgatttc cccatttggc aattcaaagg ctagggctgg tccaccagtt
    16681 tcttctgctt tttggcgcgc aacgacagca accttacggt ctgcaggtgt gataccgagg
    16741 tcgttcatga gcaactcaat tttcttgacg gcagcttcgc caactttttc agctttgaag
    16801 tcaagaactg tttgatagta acggcggatg atttcttgtt tagaagcttc gacagcggcc
    16861 tcgtcatctg taatagcgaa accaaccatg ttgacaccca tatctgtcgg tgaagcgtat
    16921 ggtgattttc cgagaatacg ttccgacatg cgtttgagca ctgggaagat ttcgatatca
    16981 cggttgtagt tgacagtggt ttctccatag gtttgaagat ggaaggggtc aatcatgttg
    17041 acatcatcaa ggtcagctgt ggcagcttca taagccaagt taactggatg atgaagggga
    17101 agattccaaa cagggaaggt ttcaaattta gcgtagccag atttgatgcc attgatttgg
    17161 tcgtggtaca tattggacat acacgttgcc aattttccag aaccgggtcc aggagcggtt
    17221 acgacaatca agttgcgact ggttttgatg tagtcgtttt tgcccatgcc tcctggggaa
    17281 atgatgtgat ccatatccgt aggatatcct ttgattggat aatgaagata agaatcaatt
    17341 ccgtttttct caagttggtt gcggaaggca tctgcagcgg gttggccagc gtattgtgta
    17401 atgacaacgg aaccaacaaa aatccctaat tcattgaatt tgtcaatcaa acgaagaact
    17461 tcttggtcat aagaaatgcc taagtcgcca cgtgctttgg aatgttcaat gttgctagca
    17521 ttaatggcaa tcacaacctc aacctgctct ttcaattctt gcaagagctt gattttgttg
    17581 tcaggttcat aaccaggaag gacacgagca gcgtggaaat cttctaacat tttaccgcca
    17641 aactctaagt agagcttgcc gtcaaattgg ttaatgcgct ccaaaatatg gtcgcgttgt
    17701 aaattcaaat actgttcaga actaaaagct tgttttttca tttttttacc tctggactct
    17761 attataataa aaaattggaa gttaggaaac tacggagcta aaaaagaaat taaaaagatt
    17821 aagcaaacgc ttgcacaaaa ttttaaaaag tgctatcata gactatagat tattaaaata
    17881 atgaggtaaa cagatgcaag aaaaatggtg gcataatgcc gtagtctatc aagtctatcc
    17941 aaagagtttt atggatagta atggagatgg agttggtgat ttgccaggta ttaccagtaa
    18001 gttggactat ctagctaagt taggaatcac agcgatttgg ctttctcccg tttatgacag
    18061 ccctatggat gataatggct acgatattgc tgattatcaa gcgattgcgg ctatttttgg
    18121 aaccatggag gacatggatc agctgattgc agaagctaag aagcgtgata tccgtatcat
    18181 catggacttg gtggtcaatc atacctcgga tgagcatgct tggtttgtcg aagcctgtga
    18241 aaatactgac agccctgagc gagactacta tatctggcgc gatgaaccca atgacctaga
    18301 ttctatcttt agtgggtctg cttgggaata cgatgaaaag tcaggtcaat actatctcca
    18361 ctttttcagc aagaaacagc cggatctcaa ctgggaaaat gaaaaacttc gccagaaaat
    18421 ttatgagatg atgaacttct ggattgataa aggtattggt ggtttccgta tggatgttat
    18481 tgacatgatt ggcaaaattc ctgacgagaa ggtagtcaat aatggtccta tgctccatcc
    18541 ctatctcaag gaaatgaatc aggcgacctt tggagataag gagctcttga cagtagggga
    18601 gacttgggga gcaacgccag agattgccaa gctctactct gatccaaagg ggcaagaatt
    18661 gtctatggtc ttccagtttg aacatatcgg tcttcagtat caggaaggtc agcctaaatg
    18721 gcactatcaa aaagagctga atatcgctaa gttaaaagaa atcttcaaca aatggcagac
    18781 agagttagga gttgaggacg gctggaattc cctcttctgg aacaaccatg acctccctcg
    18841 tattgtctca atctggggaa atgaccaaga ataccgcgaa aaatctgcca aagcctttgc
    18901 aatcttactt catctcatga gaggaactcc ttatatctac caaggtgagg agattgggat
    18961 gaccaactat ccgtttgaaa cactggatca agtagaagat attgaatccc tcaactatgc
    19021 gcgtgaggct cttgaaaaag gtgttccgat tgaagaaatc atggacagta tccgtgttat
    19081 tggacgtgac aatgcccgta cccctatgca atgggacgag agcaaaaacg ctggtttctc
    19141 aacaggtcaa ccttggttgg cggttaatcc aaattacgag atgatcaacg tccaagaagc
    19201 gctggcaaat ccagattcta ttttctatac ctatcagaaa ctggtccaaa ttcgcaagga
    19261 gaatagctgg ctaattcgag ctgactttga attgcttgat acggctgata aggtctttgc
    19321 ttatatacgt aaggatggcg accgtcgctt cctagttgtg gctaacttgt ctaatgaaga
    19381 gcaagacttg acagtagaag gaaaagtcaa atctgtcttg attgaaaaca ccctagctca
    19441 agaagtcttt gaaaaacaaa tcttagttcc atgggatgct ttctgtgtgg aattactata
    19501 aatatttttt gcagaaaaat ttaaaattga aatcgtataa aaacaaggga ggactgtata
    19561 aaaggcagaa atcctttgtt ttttataacc aaggtttata aaccttcatt ctcgaaattc
    19621 aattaacttt acaaattccc actattaagg agaaagaaga cgaacataaa gaagcgtatc
    19681 cttagtgcag gcctgacttt tacatctgat ttgcttttag ctgcttgcgg ccaatcaggt
    19741 tcagatacaa aaacttactc atcaaccttt agtggaaatc caactacatt taactatcta
    19801 ttagactatt acgctgataa tatagtcaat tgaaacaaga acaggacaaa agagcctcat
    19861 aaaaggtatt gcaacttggt aatacctttt tgaggtgctt tttgatatga gcccatgttt
    19921 tctcaatagg attgtactca ggcgagtagg gaggaagagg taaaagttta tgcccaaact
    19981 cttcacacaa gagttctagc ttccccattc tatggaatct tgcattatcc ataataataa
    20041 ccgatggtgt ggttaatgtt ggtaagagaa acttctgaaa ccaagcttca aaaaagtcgc
    20101 tcgtcatcat ctcttcgtaa gtcattggag cgattaactc accattcatt tgttagacct
    20161 gcaaccaaag aaatcctctg atatcttctt ccagatactt tacctcttct taactgacct
    20221 tttaatgagc gaccatattc tcgatgaaaa taagtatcga atcctgtttc atcaatctaa
    20281 acaggtgcta ggtgctttaa actattaaaa ttcttaagaa ataaggctac tttttctggg
    20341 tcttgttcat agtaggtgtg gttctttttt tcgagtgtag cccatagctt tgagcgcata
    20401 gtggatggta gttggatgac aggcaaattc agaagctatt tcagtcaaat aagcgtctgg
    20461 attat
//
LOCUS       DAVLRT010000031        20197 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLRT010000031 DAVLRT010000000
VERSION     DAVLRT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.031"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..619)
                     /locus_tag="VXM85_001108"
     CDS             complement(<1..619)
                     /locus_tag="VXM85_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1345699.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KRTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENY"
     gene            complement(728..1246)
                     /locus_tag="VXM85_001109"
     CDS             complement(728..1246)
                     /locus_tag="VXM85_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1345700.1"
                     /translation="MRTYENKEELKAEIEKTFEKYISEFDNIPENLKDKRVDEVDRTP
                     AENFAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1473..2342)
                     /gene="rsmI"
                     /locus_tag="VXM85_001110"
     CDS             complement(1473..2342)
                     /gene="rsmI"
                     /locus_tag="VXM85_001110"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1345701.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2345..2662)
                     /gene="yabA"
                     /locus_tag="VXM85_001111"
     CDS             complement(2345..2662)
                     /gene="yabA"
                     /locus_tag="VXM85_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1345702.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2702..3592)
                     /locus_tag="VXM85_001112"
     CDS             complement(2702..3592)
                     /locus_tag="VXM85_001112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1345703.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3589..4227)
                     /locus_tag="VXM85_001113"
     CDS             complement(3589..4227)
                     /locus_tag="VXM85_001113"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1345704.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4447..5244)
                     /gene="proC"
                     /locus_tag="VXM85_001114"
     CDS             complement(4447..5244)
                     /gene="proC"
                     /locus_tag="VXM85_001114"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1345705.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            complement(5248..6510)
                     /locus_tag="VXM85_001115"
     CDS             complement(5248..6510)
                     /locus_tag="VXM85_001115"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1345706.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASFLPRLEQ
                     VLVAERKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6520..7650)
                     /locus_tag="VXM85_001116"
     CDS             complement(6520..7650)
                     /locus_tag="VXM85_001116"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1345707.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7730..9607)
                     /gene="cbpE"
                     /locus_tag="VXM85_001117"
                     /gene_synonym="pce"
     CDS             complement(7730..9607)
                     /gene="cbpE"
                     /locus_tag="VXM85_001117"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1345708.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAETGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSLISVVKVNG
                     KKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQTS
                     DSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPSFQ
                     AGWHKSVYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFYLT
                     DSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYMGQWYY
                     FAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKANDW
                     YFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKSAT
                     IKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSESE
                     Q"
     gene            complement(9610..10497)
                     /locus_tag="VXM85_001118"
     CDS             complement(9610..10497)
                     /locus_tag="VXM85_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1345709.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLSLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10487..10948)
                     /locus_tag="VXM85_001119"
     CDS             complement(10487..10948)
                     /locus_tag="VXM85_001119"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1345710.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10945..11853)
                     /locus_tag="VXM85_001120"
     CDS             complement(10945..11853)
                     /locus_tag="VXM85_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1345711.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11846..12052
                     /locus_tag="VXM85_001121"
     CDS             11846..12052
                     /locus_tag="VXM85_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1345712.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLHYLPFSR
                     QSYFQNQFKKISGYTPLQYRKEKR"
     gene            complement(12104..12775)
                     /locus_tag="VXM85_001122"
     CDS             complement(12104..12775)
                     /locus_tag="VXM85_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1345713.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12829..13050)
                     /locus_tag="VXM85_001123"
     CDS             complement(12829..13050)
                     /locus_tag="VXM85_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV1345714.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13321..14130
                     /locus_tag="VXM85_001124"
     CDS             13321..14130
                     /locus_tag="VXM85_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1345715.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     NVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14311..15186)
                     /gene="aguB"
                     /locus_tag="VXM85_001125"
     CDS             complement(14311..15186)
                     /gene="aguB"
                     /locus_tag="VXM85_001125"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1345716.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15196..16281)
                     /gene="aguA"
                     /locus_tag="VXM85_001126"
     CDS             complement(15196..16281)
                     /gene="aguA"
                     /locus_tag="VXM85_001126"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1345717.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16278..17405)
                     /gene="nspC"
                     /locus_tag="VXM85_001127"
     CDS             complement(16278..17405)
                     /gene="nspC"
                     /locus_tag="VXM85_001127"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1345718.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17405..18664)
                     /locus_tag="VXM85_001128"
     CDS             complement(17405..18664)
                     /locus_tag="VXM85_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1345719.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(18661..19521)
                     /gene="speE"
                     /locus_tag="VXM85_001129"
     CDS             complement(18661..19521)
                     /gene="speE"
                     /locus_tag="VXM85_001129"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1345720.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDREGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            complement(19552..19689)
                     /locus_tag="VXM85_001130"
     CDS             complement(19552..19689)
                     /locus_tag="VXM85_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345721.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            19776..>20197
                     /locus_tag="VXM85_001131"
     CDS             19776..>20197
                     /locus_tag="VXM85_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1345722.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLK"
BASE COUNT         5846 a         4680 c         3591 g         6080 t
ORIGIN      
        1 tgtaattctc aagctgtgag ttcattatga tttgtaaatc cgatatatct tcagcagcta
       61 aaaacacatt ttgaaatgtt ccaagtccgt tttcaccaaa gctatattct ctcttactat
      121 acttactctt attattctct atatagttag agtcaaggtt cttaacctct tgaagttcat
      181 tttctttact tctgaggtta aaatctttta cttctgaagt taagtttttt gacttctgta
      241 agtcattttc ttttacctga aatatactca taaagtcttt aacataaatg atgttcggct
      301 taccaagtcc aagccttact ctttcgatca gtcctattcc tttttttaca tcaagctcat
      361 ctaatgtttt tatggcagtt ggctttgaga tatttcttct tttcataatt tcttcgacag
      421 taaaatagat aaatactctg ccttccttgt ctatccagtt attcttaaat gacattcctg
      481 tccgtttaag aagtatggaa taaaggataa ttgcttctgc tgataagccc cgaaattctt
      541 ctctatcaac taatatctcc ggtactttta agaagttaaa tctttccgct tctctgttat
      601 aaaaatattc aaagtccatg caagatcacc tcccttctta aaaatttgca aagaaaaaga
      661 cgatagtttt tcttctatcg cctttggtaa ccatttttat gaaatttttt aaagttaaaa
      721 atataattta taatactatc ttcttccatt ttctgatttt agttctgaaa gttccaaaag
      781 gtgcaaccgt atttacatga ataaacttat acacttccca agtcgctgtt ttagtcgctt
      841 catcagccca ctttctcata tgcggttcaa ataattcttc ctcactcaac gaatcaatca
      901 ttgcagagat agaattaata ttttcattta attttgcttt caactcttgc agagataaat
      961 gagcgtaggt atctgtgaac cactgatata attcaccaag ttgattccat ttaaatttat
     1021 ccgatggtgt ttttacttga agcccctttc tttcatcttc ttcccattta agaaccaagt
     1081 tggtccagcc aacctgataa gcaaagtttt ctgctggagt tctatcaact tcatcaactc
     1141 tcttatcttt taaattttct ggaatattat caaattctga aatatatttc tcaaatgttt
     1201 tctctatctc agcttttagt tcttctttat tttcgtatgt tcgcaaaaaa tcaccttctt
     1261 tctgatttat caatcctttg atttaaaatg accttatttt tctatttata ccttctagga
     1321 acgctataac tgctgctcca accatcggta ttccgtatgg gcttaataaa aaacttaata
     1381 ccaaagcttc tataccaagt gatacatctt tatattatca tcactaatta agttaaacag
     1441 aaacagacag aattattaca tcctgtctcc ctttattgtt tttcttccca gtcgtggtag
     1501 gcagcgtaga gctgactttt attccactgg taaatcttag cgacttcctt gatagcttgg
     1561 tttttcttca caccttgctg gatgcgggtt tgaatttcta cgaacaagtc ttcctcgtcc
     1621 ttttcctcca caccctgact ggcaccctca acaatgagaa gacattcgcc cttgagtggc
     1681 gtttcagcaa tgctttctaa taactcagag atagtacctc gttggtattc ttcatagatt
     1741 ttggtcaatt ccctgaccaa gacaactgag cggtcaccgt agacttctaa catattttcc
     1801 aacgtgtctg ctacacgatg aggtgattca taaaaaatct gtgtttcagg ataatctttt
     1861 ttcaagccaa aaaattgctt ctgctgacct gattttctcg gtaaaaaacc gtaaaagata
     1921 tgtggctgtg gcgctaaacc actggcaatc aaggcagaaa ttcctgcaga ggcacctgga
     1981 actgtcacaa ctgcaatttc ttcctcaata gctgccttaa ctaaatcatg accagggtct
     2041 gaaatgctag gcaaaccggc atcagagacc tgagcaatac tttgccctgc tttcaagaaa
     2101 ccaatcaaat caggaatttt ttccttggca ttgtgctcat gaaaactgat ctgcttggtg
     2161 gaaatgtcaa aatgcttgag caaaagacct gtattgcgcg tatcctcagc agcaatccaa
     2221 tctacttctt tcaaggtctg gatagctcga aaggtcatat catctagatt gccaatcggc
     2281 gttgccacta gatacagctt gccataggga gactgcccct taaaactttt ttgaatctgc
     2341 atgcctactc cctgtatagc aactcgtcac aaaacataca ttcctcgtcc tgctctcgac
     2401 gttgtccata aaaatcatta catacgtgaa atccatcacg gtaaatgcga cggacacttt
     2461 cacgaacatg cttggccttg acaggagcat ctgcttccac ctcacccaag cgttctcgca
     2521 acttactatt ttccaagcga agagctgtat tttcctctac caggctcttg agatttttct
     2581 tgatggcttc cacatcggct aaggttacca ataattgttg ggaaaaatca tccagcgcgt
     2641 caaataattc ttttttgtcc ataaaacagc cctttccttt ctttatcatt catttttgag
     2701 tttatatttc tttcaagacc agatattcca tggcattttg aaagctgaca ttagcttgcc
     2761 acatttttct agcttctagt aaatcttgta gaatcactct tactcttacc tgcaagaggt
     2821 cctgcccaca gagaacttca agaatccgta aaacctgatc ctgtttttcc ttatcatctg
     2881 ccaagttggc taatttggca acctgtagat aactttcttt tttcttagct actaaccaag
     2941 tcagcaggcg ttcactttca tcgaccaagg tccaaaaact tgcctgatgt gccaactttt
     3001 ctgcttcagc tcgcgattga ctaaacttag ctaaaagagt cgcttttttc ttaacaagtc
     3061 ccatttgttc taagagtaag ataagttttt cttcttgctt tttaaagtgg aagatctgag
     3121 tccgacttcg gattgtcggt aacatctttt cctcatcgct agtcaagaag aaaatataaa
     3181 cttcactctg gggttcttcg atgaccttga gcagagaatt ggctgcgttg ggatgcattt
     3241 tatccgcttg ctcgatgata aagacctgtt gctggctttc aatccctgct tgagaaaact
     3301 gtcccaccaa ttcccgaatg cgttctgtct tgatgacctg attgactggc ttaatcaagg
     3361 tgacatctgg aaactcttcc tgttcaatca gcttgcaact tcggcatttc tcacatggta
     3421 agacgccaac tttatccgta caaaagaggc tcttagctaa aaattgcgcc atttccaagc
     3481 ttccaaagaa acctgaaaag agataggcgt gattgagctg gtcttgttct aagatacgga
     3541 caaaacggtc aaactgagct ggttgccaag cctttagttg atcttgtttc atttggccaa
     3601 gcccattccg tcaaacaaga cagccttggt agtttccaca acttgctcca aagggagact
     3661 agcatcaatc ttgacaatgc gatttccctc tttatccaga agagaaaggt agccttgacg
     3721 aactttttta tgcaagtcca acccttccaa atccaaacga ttaacctcgc ggtcactatt
     3781 agcagcaata cgagccagcc cttcttccac ctcgatgtca aaatagagtg tcaaatcggg
     3841 tttgaggcca tctgtcgcaa actgattgag ccagtcaatg gcttcaatat ctaagccacg
     3901 accaaatccc tgataggcaa cagaactatc gataaaacga tccatgatga ccaacttgcc
     3961 agcttcaagg gctggaagaa ctttttccac caaatgctgt ctgcgactgg caatatagag
     4021 aagtagctct gttttagcat ccatctgagt atgacttgga tccaaaatca cttcccgaat
     4081 cttctcccca atcaagactc cgccaggttc acgggtcgtc aacacctcta ctcctttttc
     4141 ctctaaaatt ggtagcagag cctctaaaac actggtcttg cctgctccct ctggtccctc
     4201 aagagagact aaaaatcctt ttgacatgtc taactcattt cttttttact acttctattc
     4261 tatcaaaaaa ataggggttt gtgacaatct ttttgtcttc tcatttcata ctcaatgaaa
     4321 atcaaagagc aaactaggaa gctagccgca ggctgtactt gagtacggca aggtgaagct
     4381 gacgcagttt gaatttgatt ttcgaagagt atcaaccacc ataaaagagg cagataaaac
     4441 tacctcttat ttacctagtt cttgtgttcg tttataggct tgatgaactg cctccatgac
     4501 tatgcctcga aaagcatgcg cttctaggct tgctacgcca gcaatagtcg aaccgcctgg
     4561 actacaaact tgatctttca aaactccagg atgttgctgg ctttctagga ccaattgccc
     4621 agctcctacc acagtttggg ctgccatttt caaggctgtt tctcgtggta atcccgtctg
     4681 aacacctgca tctgccaaag cctcaataaa gagatagaca aaggctggtc cacagcctgc
     4741 aagacctgtc gctgcattga ttaagctttc tcctaattca accaagagac cagccttgga
     4801 taaaagctga caaaagagct cactgtcctc agccctgcaa ttaggagaca aggcataact
     4861 aatcactcct tgcccgatag aagcaggggt attaggcatc atacgaataa ttcggtgttg
     4921 acttgggata agacttgcta gtttttctaa ggtcaatcca gctgccatcg aaatcaaaag
     4981 aagactttct cttttttcaa ggatggtctg gtattgagaa agcagttcag aaaactgagc
     5041 aggcttaact cctagaaaaa tcacatctgc ttctgcaaac atttcttcat tgctggaagc
     5101 ctgaccacca aagtctgcaa tgaaagcatc taccttagct tgactacgat tggcaaggag
     5161 aatctcatct gacgtcctag tctgcaagac agattttgcc aagctagcac ccatattccc
     5221 caaaccgata aatccaatct tcatctctta ctcccttatc tgcccatcac cagtgaccac
     5281 atacttgtag ctagtcaatt ccttcaagcc cataggacca cgcgcgtgca atttctgagt
     5341 agaaatcccc atttcacaac caagaccaaa ttgtcctcca tctgtgaaac gagttgaggc
     5401 attaacatac accgctgcag agtccacttg atctgtaaag tatgctgcag cttcagcatt
     5461 ttccgtcaca atggcatccg aatgatgggt actgtgggat tcaatatgcg caaccgcttc
     5521 ttctaaactg ctcacaacct taacagctag aatatagtct aaaaactcgg tatcaaagtc
     5581 ttgtgcttga gcagcttgac ctgaaacaaa ctggcttgct ttgctatcta ggcggaattg
     5641 aattggttcc agtccagctt ccttacgctc tgcaaccaac acttgctcca agcgaggaag
     5701 gaagcttgct gccttgtttt catgaaccag cagaacctcc atggcattac aaacagaagg
     5761 acgactggtt ttagcattgt tgatgataga cagcgccttg tcttcgtctg catccttatc
     5821 cacatagaca tggacaatcc cagtccctgt ctcgataaca ggtacaatcg cattctcaac
     5881 cactgcattg atcaagccag ctcctccacg aggaatgaga aggtctagat agcccttggc
     5941 cttcatcata gcataactac tttcacggct agtatcctcc accagttgaa tcacatttgg
     6001 atgaatagta gtcgtctcca agcccttctt caaggctgtg acaatggcat gggttgtttg
     6061 ataggcatcc ttaccactac gaagaacaac cgcatttcca ctcttaagag tcaaagcagc
     6121 cgcatcagac gtcacatttg gacggctttc atagataata ccgatgacac ccatagctac
     6181 acgttttttt gtgataacca aaccattttc aagctgactt gtttctaaaa cttcaccgat
     6241 tggatctggt aaggcaacca cttcacgaat tcctcttgcc atcgcttcta tacgatctgc
     6301 atccaaataa agacgatcca acatcacatc tgagattttc cccttagccg ctgccatatc
     6361 gagggcatta gccgctaaaa tttcctcagt agcagccact aagtgatcag ccatggctag
     6421 caaggcttgg tttttcactt cttcactagc tgtgttaatc gattttttaa cagcctgtac
     6481 ctgttcaaat tgttcttgtc tactcaccat agtttacctc taaaattctg taaaaagtag
     6541 ttggatttca ggagtaatgg aaatccagtc gtcacggtaa atcaagacac ccttggcttt
     6601 ttgagaacgc aacatatcct ccaaagcaga tgctccaaat tgcacgcgtc cttttccaag
     6661 tgattttcca ctttccttgt caaatactgt cacgatatca ccgtaagaaa agactccttc
     6721 tgcttcaacg ataccagata agagaagact ctttccatgt tgagagagag cttccgcagc
     6781 ccctttatca acccaaatag aaccttgact ctgagcatag aaggcaagcc attgtttctg
     6841 ggtacgaagc cccttctctt gagcaacaaa gtaagaacca tcctcggtct cctctgccgc
     6901 ctcaatcatg gaatctgatt tcaaggatga gcagatataa acaggaactc ctgattccgt
     6961 cgcgatagtt gcagccttga ttttggttaa cataccccca gttccgtttg acgaaccagc
     7021 tccaccagcc atatcaataa tctcacgatt gatggtctcg attctctcca agcgtttggc
     7081 tcttggatct gaattaggat ttccagtata gagaccgtcc acatctgtca agagaaccaa
     7141 aaggtctgct tggaccatcg ccgctacttg ggcacttaga gtgtcattgt ccccaacctt
     7201 gacctcatca ataacgacac tatcattctc attgatgata ggaattgccc cacggttgag
     7261 taaaaccgac aaagcctgat gggcattttt ataacgacgc ttatccacaa agtcatcttg
     7321 ggtcagcaag atttgtgcag aaacgatttg acgcaagaga agattggtcg tatattcttc
     7381 caacaaaagt ccctgcccta ccgctgctga agcctgttta tcagcaatct tagtcggacg
     7441 ctttttaaat cctaaggctc caaaaccagc cgcaatggca cctgaagaca ccaaaatcaa
     7501 ctcatgacca gcctcgtgca gcatagccaa ctgctgggta atatccttta ccttactacg
     7561 tgataaactt ccatcctcat ttgtcagaga agaagtaccc accttaaaga caatccgttt
     7621 gtatttcata gtctcactcc gattattcat atttatccat tataacatga ataacaggaa
     7681 atagaaaaga gttgatagga aaaagaaggc cctaaaacct tcttttctac tactgttctg
     7741 attccgattt gtttacactc gtttcagagc tttgtgaagt tgaggatgtc aaatcttgac
     7801 tagttgaaga atttgtaaca ctttcatgtt gactcgtttc tttattttca agatcctttt
     7861 tcactacttc tttggattct tttatttctg aatgacttgt agttttaatt gtagcagatt
     7921 tctcgatcga aacatccact aaccaggcac cacttgaatc cacagtatag ccttctggtg
     7981 tcttaccatt cacaagtaat tgaccatttt ctttcaagaa gtaccattta tccttgtcct
     8041 tgatccaacc cacagctcgt gaaccgtctg tcttgtagaa ataccaatca tttgcctttt
     8101 tatgccagcc ttgcttcata gctcctgaat cttccagata ataatgttga ccagcaactt
     8161 ctatctcacc tgtcttcatg acaccagtag aatccatata gtaccaggtt tctttatctt
     8221 ttacccagcc cattttcatt tcccctgatg gagcaaagta ataccattgc cccatatatt
     8281 gaagccatcc tgtcttcata gaaccatcaa catccaaata ataccactgc tctttatctt
     8341 gaacccaacc aatttccatc tgattttctt tgttaaaata ataccagatt ccatcaattt
     8401 tcttccaatt tttagcagaa gcaccagagt ctgtcaaata gaaccaatga ttgttccatt
     8461 ttttccattg attctgtaac aagatacccg tttggttaaa gtaataccat tcaccttcga
     8521 tttcattcca accgacagca tactctcctg tagaatcagg cgcttgatac caccagttcc
     8581 catatacact cttatgccaa ccagcttgaa aacttggaat cggcttgtag gatgttgaaa
     8641 tattgacaaa accgtctttt cgaatatcaa aaactgtcgc atcatagtct ttgctggctg
     8701 cgttgattct ctcaattcct cgttctttga gccaattaac atactcacta tcaacaccat
     8761 ttttccaagg tagactatcc gaagtttgaa caatcaaact cggactcaaa tttttaatga
     8821 aatccttggt atttgatttg ttggtatcat ggtgatgatt aaacttcatc aaatcaactt
     8881 ttccaatgag aggaccatac ttgtcttctg ctccatgaac attatctaaa tcgcccccaa
     8941 ggtaaatttt cttgccattg actttcacca cgctaatcaa ggaattgtca tcccaaattt
     9001 tctttaattc acccgatgaa tcagtttcat tttcataatt atagagctga atatccatgt
     9061 ccccaaactg aaaatgagca tccccttgtg tgatattttg aataactgaa acacctgttt
     9121 ctgcagcagt ctgtaaaacc ttatcatagc catacagatt atcccataga cgttcagaat
     9181 tagtaatacg actatcacta tatttcttaa gatagactcg gtcaactgga taggtagaca
     9241 gtaattcatc aacatttcca atatgatcac tgtgggtatg ggtcaccaaa ataaaatcaa
     9301 gtttttggac acccaattcc ttcaaacgac gaaagacacg gtctgttaga acatgcttat
     9361 aagacgtttc aattccttct ctccatggat agcgagaatc acttccatct gggaaatcat
     9421 aatcttctcc tgtatccacc atggcaaaat gtccattgct ttcaagaata atcgcatcac
     9481 tgccaccttc ttgaacattg ataaagtgga ttttatttcc tgaactttct tgagcctgaa
     9541 cattcccata ccatgacaaa cctaaaaaag cgcctacaag tgctaaacta gttaatttct
     9601 ttttcattct tactttctca atctctccaa ggtttcctta aaaatctctg ggatatctgc
     9661 tttaaattcc aaggtcttac ctgttctcgg atgagtaaaa cctaaagtct tggcatgaag
     9721 aaattgtcca tgtcctttca aagtcttgcg aggaccatag acctcatcac cagcgactgg
     9781 atggccgata taagccatgt ggacacggat ttgatgagtg cgccctgtct ccagttgcaa
     9841 ctctactaag ctataatcgc caaagcgttc caagacgtga aaacgcgtca ctgcaggctt
     9901 ccctttagca gttacagcct gtttcttacg gtctttttca ctccggccaa tcggcgcttc
     9961 aattacacca cgatcattag gcagatttcc atgaacaatc gcccaatatt tgcggagaga
    10021 ctttttatcc ttgagttctt gggcaagtgc tagatgcgca tcatcgtttt tagcaatcat
    10081 gagaagacct gacgtatcct tatcaatacg gtgaacaatc cctggacgca gaaccccatt
    10141 gatacccgac aagtccttaa tatgatacat gagggcattt actagggttc cactggtatg
    10201 accagcactc gggtgcacaa ccattccctg aggtttgtta acgacagcca catcctcatc
    10261 ttggtagact atttctagcg aaagatcctc agccacatac tctaatacct ctggttctgg
    10321 cacatggtaa gtgacgacat caccctcttg gactgtgtat ttagctttct tgacttgacc
    10381 attgaccaag acctggcctg atttaatttg ttcattcgcg agactacgtg ataattctga
    10441 caaatctgac aaagccttat ccaaacgcag accaccagtt tcaattttaa tttccattta
    10501 tttcctcttt tagcattgca atcaataaaa taatcactcc aaccgtcaga tagctatctg
    10561 ccacattgaa aattgcaaag ttgataaagt caaggtggaa catatccaca acaaagccct
    10621 gactgaccct gtcaataaag tttccaagac cacccgcgat tattagagtc aaacccaaga
    10681 ccatccagaa tgagtcctcc atgtgtttat gtaaatacca aatggcacct atcacgacaa
    10741 ccagagtaat gacagcgaat aacagctgct gatcttgtaa gatagaaaag gctgcacctc
    10801 gattttgcag gtaggtcaag ctaacgaaat tgggaatcca ggagcgcact tcacccagtg
    10861 gaatctgctg gacgatatag gatttgacca actgatccag cccaattaaa agcagtacaa
    10921 tgactgccac tattgctctt tttttcatga tttcctcttt tgatcaaaat attcttgcat
    10981 gacttctacg aagagagtcc cagcttgact aagctccact tcttcacgtt taacatagac
    11041 catgcggtta tctaggttat ccttgagacg aataactgta atgccattaa cactgtcact
    11101 atctaaaaat ccagaacctg tcgcataggc gtccgtccgc tccaaaatac cattcaaggt
    11161 ggcacggtct gtcacattaa acatctgtga gctagcgctg gtatcgacaa agttctctga
    11221 ataataaagg tactcgtctt tctcttgagt gaaacgaacc gttggtaaat ccgctaaatc
    11281 ctccatgact aattcctctt tctgggctaa aggatgaccc tcacagagat aaatatgggt
    11341 atggaaagga atcaattcga tgacctccag acctaacttt tcaacccgtt gcataatccc
    11401 ctttttattt tgattgttga ggtagataat cccaatctca ctatgccctt gcgccacttc
    11461 atctaatatt tgaacagtag ttgattcaaa aatacggaag ttcttatagt caggatagcg
    11521 ctctgaaaag gccgtaatag ttggtggtaa gaagtcatag tgctggctag caacggaaaa
    11581 ttcatctttt tcttcttcag gattggcata ctgattttga aaaatatcaa atcctttaac
    11641 caattcttgc gctttttcat aaaattccat cccacgacgg gtcaagaaag tccctgagct
    11701 ggtccgacgg aaaatcttaa agcccaactc tttttccaaa tcacgaacag aaatagacag
    11761 actcggctga ctaacataca tcttttcagc agcttcacga aaagtaccac tattggcaat
    11821 agccacaaca tagcgtaatt gttgaatgtt catcttctac ccccaacttc tttatctttt
    11881 cattatacca tattttagaa gttttccaaa aaggaaaaaa gaacatccta ttcttcttaa
    11941 ctatcttcac tatcttcact atctgccttt ctcacgccaa tcttattttc aaaatcaatt
    12001 caaaaaaata agtggctaca caccacttca gtatcggaaa gaaaaacgtt gactatttgt
    12061 gaaaaaagaa aatgccggaa aattccgaca tttttttagt aagctaactt cctgaaaata
    12121 ggagtaacca aacatcccag aaggcagtga tattgatgag aatatgaact agtattggat
    12181 agtagagatt tttagtcatg cgatacaata gagctaaaat cagacctcca ctagcataaa
    12241 tgaaaaaagc aagaggagtt aaagcaaaat tgatatgaat ataaccgaaa ataatagcag
    12301 aaagcaaaac atctccgtac caaggtgagt ttttgaaaaa ggttgtcata agcacacctc
    12361 gataaatcaa ttcctcagca ataggggcga tgaagcaaac gatgagcaag aaataaggga
    12421 actcctgtct ccccatcatt tctaccgttt cattcaaaga aatctgattt gaagacaggg
    12481 atatgaaata cgaaaagagg aagtcagaca tatatgaaat gatgtagccc agtaaaaggt
    12541 agatgaagta cctcagctgc cacttgaaat gaaaaatttt ctttcctgca aaaaccagta
    12601 gatagatgac cgctaagaaa agaactccgc tctccatcaa aagcagaatc tgaaaaaatt
    12661 cacgacttgc tggtagatag ggctttgcga ggtgattgaa aactctccaa aaccaagttg
    12721 atttgtaaaa aattaaggac aatgctagta aaatttgaat agcccgtttt ttcatattaa
    12781 atcctctgtt ttctcccctt gtttcttcat attcctaata aattttactt aaaatctcgc
    12841 agcacttcct ttgcaaagat gattgcctcc tccaatatat cctaaatcat aggtgcctct
    12901 gggacaaaat ccatgaaccg gcagagttaa tactggttct atttcccaat cgtacctacc
    12961 attaggattt ttaccatagg gatctgctcc accttgtatc agctctaatt cttcatttgt
    13021 tagatttata ttttttgtca ttaaaatcat gatatatttc ctccattatt cttcagatta
    13081 gttgagtaat ctactactca tacctactta caaaaaaact attttaagct agttttttta
    13141 tgcttgtcta gttgcaatat tgacaagttt agtatatcat atctttgcca aatatcctct
    13201 tcaaatcttg aattgtcatc gaaacgtcct aaactgcaaa atcaattagt ctcaagcttt
    13261 ctatcaattt cttctccaaa atatgctata ataatagcaa aagataaaga aggaagacct
    13321 atgattaaac tactagcctt ggatatggac ggcaccctcc tcaatgaagc caaggaaatc
    13381 ccacaagctc acattactgc tattcacaaa gccattgaaa aaggtgtcaa actggttctc
    13441 tgtacgggtc gccccctttt cggtgtcctc ccctactaca aaaaactggg gcttgacctc
    13501 cagaacgaat atgttattgt taacaacggt tgttcaactc accaaaccag tgattggggt
    13561 ctagttgact ggaaagaact gagtccagct gacatcgaat acctctatga ccttgctgaa
    13621 aagagtaatg ttcagttgac actttttgac gagtcacatt attttgttct cggtggcaag
    13681 cccaatcaag ttattgaaaa tgatgctaaa ctagtatttt cagacctgac tgaaatttct
    13741 cttgaggaag cgactagtgg aaagttccgg atgttccaag gtatgttttt aggaacaaaa
    13801 gaacaaacag acgattttga gcagcgtttt gctgaagagc tttgccaacg attcagtgga
    13861 gttcgttcgc agcctgtcat ttatgaagca atgccacttg gaacgacaaa ggctactgct
    13921 ctttcacgac tagctgagat tttgaagatt gattcctcag agattatggc catgggcgat
    13981 gctaataacg atatcgaaat gctccagttt gcagggcttg ggattgcaat gggaaatgcc
    14041 agcgattatg tcaaatctct tgcggatgcc gttacctcaa gcaacgaaga agacggcgtt
    14101 gcgcgtgcta ttgagaaata tattctataa aaaagaaaag caaatagaca gaagttactg
    14161 ctatttgctt ttttgctaat attttaaaat acggactaat caatagacaa gaatagcgaa
    14221 tcataaaaat cgatttacta gatgccatag tatcttatag ttgctaataa gaagttagtc
    14281 tagcagaatg aatctctcat ttctcccaca ctaatctgta atttgtctat acatttctgg
    14341 tcttctatct cgaaacaagc cccagtttag gcgttcactc gctcccttgt ctaggtcata
    14401 agtagctaac agaacagctt cttcttgtct ttcagctcgt tctagaatag ctcctgtttc
    14461 atccgtcata aaggaggaac cgtagaagtc aagactggaa ctctgtccgc cattttcctc
    14521 actaggagta acctcctcta aaccataacg attggctgcg atgactggaa caatattcgc
    14581 tgctgcgtgc ccttgcatag tacgttgcca gtgaccacaa ctatctgtat ccaaaatcgg
    14641 ctctgaaccg atggctgttg gataaaagag caattctgcc ccattcagag caagacagcg
    14701 ggctgtttcg gggaaccatt gatcccaaca gataccgata ccaatcttag catagcgagt
    14761 attccagacc ttgaaaccag tgttaccagg cgtgaaatag aatttttctt gataataatg
    14821 gtcatctggt atgtgggtct ttcgataaac gcccagcact tccccatctg catcaataac
    14881 ggcaatagag ttatacaaga cattaccatc tttttcatag aaactgattg gtaaaacaac
    14941 ttgtagttcc ttagcaatca ccttaaaatg ctgaatggca gtattttccg ctacagattg
    15001 ggcatactgg tagtagtcat actgacgttc ctgacagaaa tagggatgtt caaacaactc
    15061 gggcaagaga ataatttggg ctccttgctc agcagcctga cgtactaaac gctctgcggt
    15121 ttggatattt gttgccacat ccttagcgca ttgcatctga atggttgcaa ctcttacatt
    15181 tctcatcttt ttctcctatt ctggaatttg ttgggtgata cagtggatat tgccaccacc
    15241 taagagaata tctctggctg gtattccgac aactttacgg tctgggaaac acttgctgag
    15301 gatatctaag gccacttggt cgtttacatc ctcaaactgt ggaaccaaga cagccttgtt
    15361 ggcgatataa aagtttacgt aggaagctgc tagtcgttca cctgcgtatc gcttttcttc
    15421 tccttcttca tagatgtagc ctggcaaatc ttcttctgtc acaacttgtc gaactgcagg
    15481 gataggcaat ttatgaatgg tgaagtgaca accttttgca tctgtttcct gttctaagag
    15541 ttcgagatct gcttttgaca tggcatactg gggatcattt tcgtcatctg tccaagctaa
    15601 aacaacctca gcaggaccaa caaaggcagc aacattatcg acgtgttcat tggtttcatc
    15661 ctgataaata ccataaggaa gccaaataac tttttcagca ccaagacttt ctaataatgt
    15721 gttttcaatc tcctctttag tcaggttagg attgcgacca ggactaagca agcaactttc
    15781 agttacgaga atagttcctt gaccatcgct atggattgcg cctccttcca gtacaaaagg
    15841 tttagcatca tagacaggct tttccaaggc ctcagcaaaa cgactggcta cttggtcatc
    15901 ctcttcataa tcttgataaa gaccatcata ggtgcctccc caagcattga aggcccaatc
    15961 cacggctaat ttcttacctt tatcattgac gagaatggtt gggccagtat cacgcgccca
    16021 ggcatcattg gtgggaatgt ctaaataaac aaccttgtct ccaagatagg attgggcttc
    16081 agatagatag gcctgctcca ccaaaagata gactctttcc ccttctgcta tggtctcgat
    16141 gatctgagta aatgatcttt tagcagcctt tccttgaaaa ggccatgatc ctggtcgagt
    16201 cggccatatc atgagggtac catgatgggg ttcgtactct gctggcatgt gatagcctaa
    16261 tttttttgga ctgtccatca tgataatctc cctttaaagt cttgatagcc aaaagctttg
    16321 agtaagctac agtctccctg ttcgtccatg agatagagac ttggcaatcc aataccatta
    16381 aaggtattat ttttgacaaa agaataaatg gccatgtctt gaaaataaag tctgtctccg
    16441 atttggactg gattttcaaa actataatca ccaatcacat cgcccgtcag acaggtatta
    16501 gaagaaagtc tgtaggtatg ggctttttcc tgtgcctcaa agccatttct caaaggtgga
    16561 cgatagggca tctcaagtac atcaggcata tggcaggtcg cagaggcgtc taaaaccaag
    16621 atttccatac cattttctac aatatctaat acctcagttg ctaaataacc cgcattaagc
    16681 gcaatggctt caccaggctc gatatagatt tcaagattgt aagtttttcg gatacgcttg
    16741 atttctgaaa tcagcaaatc cacatcgtaa ccttctcttg taatatgatg accaccaccc
    16801 atattgagcc attttacctc atgtaagtag ggaccaaact gttcttctac tgctttcaaa
    16861 gttgtttgta aatcatctgc tccctgctcg caaagggtat gaaaatgaag tccgtcaacc
    16921 aaatctagca aatcactcgg aatcttgtct atagtaactc caaagcgaga accaggtgca
    16981 caagggtcat agagcgcgtg atctccttga gttgaacact gagggttgag gcgcaaacca
    17041 acactgacac cagcctctcg acaacgagcc ccatgcttac gcaactgtct ctctgagtta
    17101 aagacgatat ggtctgttat cccaagcaat tcctccatgt cctcatcctt gaaagctggt
    17161 gcaaaaacat ggacttcacc aggaaattct tcccttgcca atttggcctc atagagtcca
    17221 ctagctgtcg tacctgatag atactggcta atcaagggat aagttttgta gagggaatat
    17281 gccttctggg caagcaagac cttgcaaccg gcctcttctt gtacatattg tagaatgcgg
    17341 caattagctt ctaacttggc caagtcaata acataggctg gtgttggtac ttgttctaac
    17401 ttcattagtc caccatttgt ggattttcaa ccacaaccca tggcaaacca tactcattca
    17461 aagcttccat gaatggatct ggatctaact cctcaaggtt atacactcca gcttgtttcc
    17521 aagttccgtt catgactaat tttgtcccaa tcatggctgg aactcctgtc gtataagaaa
    17581 tagcttgcga accaacctct gcgtaacatt cctgatggtc gcagacattg tagatataga
    17641 tagtcttttt gacaccgtct ttgacacctg taaagataca tccaatattg gttttaccta
    17701 cagtacgtgg accaaggcta gcaggatctg gaagcaaggc tttcaaaaat tgaattggaa
    17761 caatttcttg gccgttaaag ttaatggtat ccgtacgaag gagtccaaca ttttcaagac
    17821 atttcatgtg cgtcaagtaa gattgaccaa aagtcataaa gaaacgaatg cgtttgactc
    17881 caggaatgtt cttggccagc gattcgattt cttcatggtg aaggagatac atatcttttt
    17941 gtccaacttg agggaaatca tactcacgct tgatagacat agcttcgact tcgacccatt
    18001 tcccatcttc ccagtaagaa cctggcgcag aaacctcacg gagattaatt tctggattaa
    18061 agttggttgc aaatggataa ccgtggtcac cgccattaca gtctaaaatg tcgatataat
    18121 ggatttcatc aaaatagtgt ttgagggcat aagctgaaaa gacactagtt acacctgggt
    18181 caaaaccaga accaagaaga gcagtcaagc ctgcttcttt gaatttctct tgataagccc
    18241 actgccatga gtagtcaaag taggctgtaa aaccaagttc cttacaacgt ttttcgtaga
    18301 tagcacgcca ctcagggtct tctgtgtctt ctgcttcgta gttggctgta tcgatatagt
    18361 gaacacctgt tgccaaacaa gcatccataa tggttaaatc ttgataaggc agagctacat
    18421 tcaaaacagc ttctggtttg tagctttcaa tcagggcaat cacttcttca accttgtcag
    18481 catcaagtgc tgcagtttca attttagtac ttgttttgcc ttctagcttc gctttcaagt
    18541 catcgcattt tgacttggta cggctagcaa tcataatctc tgtaaatgtt tcgctatctt
    18601 gacaaatctt tgaaatagca acttgggcaa cgcccccaca accaataact agtaaacgac
    18661 tcattttttt ccttcctctt cttctaaaat gtcctcaaca tacttgggca acataaagga
    18721 tcccacgtgt aagtttgcag tgtagtattc tgtgaaaagc tggcgttttt tccaaccttc
    18781 cctgtcaaaa tctttgacag ggtggtattt tttcgatgca aatccaaaca accaatagcc
    18841 agctgggcta gttggaatat gggcctgata aacccgactg attggaaagg cttgattgac
    18901 cttgcggtgc atgcttcggc aggccgactc atcctcgtca aagaagggac tcccatgctg
    18961 gtaaatcatg atgccgtctt ccttcagagc tcgataacta ttgccgtaga attctttggt
    19021 aaagagtcct tccgtatggc caaatggatc tgtcgcatcg ttgatgataa tatcgtaatc
    19081 atcttcgcag tttcgcaaaa agcgtagccc attttggtag taaatggtaa cacgaggatc
    19141 atctagccct gcagcaaagt ctgggaaata ctcacgacag acctcgacca acatttcatc
    19201 cggttccacg atatcgattt gttccagttc aggatagagt gttaatactt gggcaacacc
    19261 gccgtcacca cccccaataa ccaatacttt ctttggattt gggtggacag ccatgggaac
    19321 gtggacggtc atttcattgt agacgaaatc atccgcatct gagaacaaga catggccatt
    19381 taaaatcagt attttcccaa aagctggcgt atccaagact tcgatatcct gccattcact
    19441 ttttccagcg taaagttgct tggctgttct cagagacaat ttgacatctg gagtatgaac
    19501 ttcagaaaac cataaatcca tctatttctc cttatattat atttagcgca gttagctact
    19561 gccaaagccc aagtggtata cttggaataa gccactgtgg attagttcat ttcctccaat
    19621 cgcttgacga tttccgaaag gctctaacca cagcttttgt ttcaattggt aatgagtttg
    19681 ataacgcata gacgttttat ctcgtctaag gtaacaaggc aaaaagttcg tggataacca
    19741 agtagacttg aaatactata tctggaggta ctcccatgct ttatgtgggc attgatatcg
    19801 ctaaaaataa acacgatgtt acagccttga atgttccagg aaaaactgtt cttaaaccac
    19861 tcactttttc aaataataaa gctggttttg aactcttaga tctgtctctt cgacagctca
    19921 accaagactg tctcatcgct cttgaagata ctggacatta tgcctttaat ctactgaact
    19981 tcctgcatga acaaggatat aaagtttaca cctacactcc tttgctcatc aaggaatttg
    20041 ccaaatcatt atcacttcgc aagactaaaa cggataagaa agacgctcat ggcatcgctc
    20101 ttaaacttct ttctgacccc aatcgtgaac aatttcaaca cgataatcgg caagtagaac
    20161 taaaaatact ggctagacat attcatcgtc tcaagaa
//
LOCUS       DAVLRT010000032        20075 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLRT010000032 DAVLRT010000000
VERSION     DAVLRT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.032"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(85..1026)
                     /locus_tag="VXM85_001132"
     CDS             complement(85..1026)
                     /locus_tag="VXM85_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1345723.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VXM85_001133"
     CDS             complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VXM85_001133"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1345724.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            complement(2952..3032)
                     /locus_tag="VXM85_001134"
                     /pseudo
     CDS             complement(2952..3032)
                     /locus_tag="VXM85_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3074..3304)
                     /locus_tag="VXM85_001135"
     CDS             complement(3074..3304)
                     /locus_tag="VXM85_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345725.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(3322..3963)
                     /locus_tag="VXM85_001136"
     CDS             complement(3322..3963)
                     /locus_tag="VXM85_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1345726.1"
                     /translation="MKLLKNLGWILLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(3975..4709)
                     /locus_tag="VXM85_001137"
     CDS             complement(3975..4709)
                     /locus_tag="VXM85_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1345727.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(4712..4909)
                     /locus_tag="VXM85_001138"
     CDS             complement(4712..4909)
                     /locus_tag="VXM85_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345728.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(4909..5115)
                     /locus_tag="VXM85_001139"
     CDS             complement(4909..5115)
                     /locus_tag="VXM85_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1345729.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(5125..5439)
                     /locus_tag="VXM85_001140"
     CDS             complement(5125..5439)
                     /locus_tag="VXM85_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345730.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(5706..5783)
                     /locus_tag="VXM85_001141"
                     /pseudo
     CDS             complement(5706..5783)
                     /locus_tag="VXM85_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5780..7069)
                     /locus_tag="VXM85_001142"
     CDS             complement(5780..7069)
                     /locus_tag="VXM85_001142"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1345731.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(7147..8394)
                     /locus_tag="VXM85_001143"
     CDS             complement(7147..8394)
                     /locus_tag="VXM85_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1345732.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNAYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLDLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(8585..9436)
                     /gene="rgg"
                     /locus_tag="VXM85_001144"
     CDS             complement(8585..9436)
                     /gene="rgg"
                     /locus_tag="VXM85_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1345733.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELLV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            9560..9901
                     /locus_tag="VXM85_001145"
     CDS             9560..9901
                     /locus_tag="VXM85_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1345734.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIEDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            9959..10039
                     /locus_tag="VXM85_001146"
                     /pseudo
     CDS             9959..10039
                     /locus_tag="VXM85_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10155..11858)
                     /gene="ilvD"
                     /locus_tag="VXM85_001147"
     CDS             complement(10155..11858)
                     /gene="ilvD"
                     /locus_tag="VXM85_001147"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1345735.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIIDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(12091..13023)
                     /locus_tag="VXM85_001148"
     CDS             complement(12091..13023)
                     /locus_tag="VXM85_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1345736.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     NFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(13020..13877)
                     /locus_tag="VXM85_001149"
     CDS             complement(13020..13877)
                     /locus_tag="VXM85_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1345737.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(13881..15227)
                     /locus_tag="VXM85_001150"
     CDS             complement(13881..15227)
                     /locus_tag="VXM85_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1345738.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(15240..15524)
                     /locus_tag="VXM85_001151"
     CDS             complement(15240..15524)
                     /locus_tag="VXM85_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1345739.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(15528..17558)
                     /locus_tag="VXM85_001152"
     CDS             complement(15528..17558)
                     /locus_tag="VXM85_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1345740.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            17749..18756
                     /locus_tag="VXM85_001153"
     CDS             17749..18756
                     /locus_tag="VXM85_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1345741.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            18759..18959
                     /locus_tag="VXM85_001154"
     CDS             18759..18959
                     /locus_tag="VXM85_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1345742.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            19111..19293
                     /gene="rpmF"
                     /locus_tag="VXM85_001155"
     CDS             19111..19293
                     /gene="rpmF"
                     /locus_tag="VXM85_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1345743.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            19309..19458
                     /gene="rpmG"
                     /locus_tag="VXM85_001156"
     CDS             19309..19458
                     /gene="rpmG"
                     /locus_tag="VXM85_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1345744.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(19938..>20075)
                     /locus_tag="VXM85_001157"
     CDS             complement(19938..>20075)
                     /locus_tag="VXM85_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345745.1"
                     /translation="ESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT         6396 a         4306 c         3520 g         5853 t
ORIGIN      
        1 agatagaact gacgaagtca gtaacataca tacggtaagg cgacgctgac gtggtttgaa
       61 gagattttcg aagagtatta atcactaatt atctatctca acaaatcttc ctagaatatg
      121 aacattttcc gagacagaga caaaggagct tggatccact tgtgtcataa tctgtttaaa
      181 ttcattaaac tctgcacgtg taatgacagt gattaaaact gcctttctct cgtgattata
      241 ggttccttct gcatcatgga tcatggttgc tccgcggtgc aattttttat ggattttttc
      301 aattaccttc tctggatgat ttgtcacaat catggcctgc atacgctttt gcttagtaaa
      361 gactgcgtct gtcacacggc tagagacaaa gatggtaatc atagaataaa gagcgtattt
      421 ccaaccaaag gtcaaacctg ctatcagcat gatagttcca tttaccaaga aagaaatact
      481 accaacattc ttacccgttt tcttgcgaat agtcaggctg acgatatccg tcccaccact
      541 ggagatattg tttcgaagag caaaaccaat ccccaaaccc ataacaacac ccccaaaaag
      601 ggcattgata atgggatcct ccgtcaaggt tgccacaggg acaaactgga taaagaagga
      661 actcatagat accgtgataa aggtaaagac ggtgaactta tggccaatct gataccaagc
      721 taagaccatc aaagggaagt taatggcgta gaagcttagc gaaatcggaa tatgaaaacc
      781 aaaccagtga ttactcaagg cagagataat ctgtgccaga cctgttgcac cactcgaata
      841 cacatgccct ggttggaaaa agaaattaac tgctactgct gataaaaaac catagaccag
      901 agaggccgaa atcttctcat catacttttc tcgagagata ctttgtaaga cacgtaaaat
      961 ttttatctga taagcaaagc ggcgcagata atagcgccac cgcttaattc gttttgcttg
     1021 tttcatcttc ttctacttgt aagctgagtt cctctagttg tttgagagcg actgttgatg
     1081 gagcttgtgt cattgggtca gttgccttgt tgttcttagg aaaggcaatg acttcacgga
     1141 tattttcttc tccagcaagc aacatgacaa aacggtcaag cccgatagcc aaaccaccgt
     1201 gtggtgggaa accatagtcc atggcttcaa gaaggaaacc aaactggtca ttggcttctt
     1261 cagctgagaa accaagagcc ttgaacatgc gttcttgaag gtctttttgg ttgatacgaa
     1321 ggctaccacc accaagctca taaccgttca agacgatatc gtaagcaatg gcacgaacct
     1381 tagccaaatc accttctaat tcgtgagcag tctcttcctg tggaagtgtg aatggatggt
     1441 gggcgctcat gtagcggcct tcttcttcag accattcaaa catcggccag tcaaccaccc
     1501 aaaggaagtt gaacttatca ttatcaatca agccaagctc tttagcaata cgtccacgaa
     1561 gggcacccag tgttgcatta gccacttcaa gcgtatccgc cacaaagaga accaagtcct
     1621 tatcttcaag agcaagcgct gttgtcaatt cttcttggat accagtcaag aacttggcaa
     1681 ctggtccgtt taattctcca tcaaccacct tgacccaagc aagacctttg gcaccatact
     1741 gtttggctac ttccgtcatc ttgtcgatgt ctttacgtga atagttgtcc gcagctcctt
     1801 tgaccacaat cgcttttaca gcaggtgctt ctgaaaagac tttaaagtct acacctttga
     1861 ccacttctgt caagtcctga agcaacatgt caaaacgagt atctggcttg tcagaaccgt
     1921 aaagagccat agcatcatcg tatttcatac gagggaacgg tagcgttact tcgatgcctt
     1981 ttgtttcctt catcacgcgc gcgatcaagc cttctgtaat atcttggatt tctttctcag
     2041 taaggaagga cgtttccaag tcgacctgag taaattcagg ctggcggtct ccacgcaagt
     2101 cctcgtcacg gaaacattta acgatttggt agtaacggtc aaaaccagca ttcatcaaga
     2161 gctgtttcgt gatttgtgga ctttgaggaa gagcgtaaaa atgcccctta ttaacacgag
     2221 acggcactaa ataatcacgc gccccttcag gcgttgactt agaaaggaat ggtgtctcca
     2281 cgtcgataaa ctccaactca tccaaatagt tgcggataga gtgggtcacc ttggcacgaa
     2341 gtttaagatt ttccaacatt tctggacgac gaaggtcaag gtaacggtaa cgcaaacgtg
     2401 tatcgtcatt tgcctcaatg ccatccttaa tctcaaatgg tgttgtctta gctgtgttaa
     2461 gcacaataag agctgtcacg tttaactcaa ccgcaccagt tggcaactta tcattggctt
     2521 gctcacgcgc agcgacctga ccagtcacct caataacaaa ttcgctacga aggctttcag
     2581 ctgttgccat aacctctgca gatacttttt cagggttgat aaccaactgc atgattcctt
     2641 cacggtcacg aagatcgata aagatcaaac caccaaggtc acgacgacgg ccaacccatc
     2701 ctttcaaggt tatttcttgt ccgatgtgtt cctcacgaac acgaccagca tacatactac
     2761 gtttcattat ttctctcctc ttttattctg ttactatttt accataaaag cgcaggctct
     2821 tcatgaaaat catcagaaaa gtttgccagt ctttaaaagt caggtgaaag ccctaaaaat
     2881 tagcgctaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt accgtaggta
     2941 tggttactga ctccgtcagt tctatccaca acctcaaaac agtgttttga gcaacctgcg
     3001 gctagcttcc tagtttgctc tttgattttc attgagtata atacaaaaat ccgatgaact
     3061 tcaccggact cttttatttt gaatttttgc ctgctttacg cttttcagcg atttcggctg
     3121 cctttcgagg caagacaatt tccgttatgt aagccgtccc aaaacgcagt acacctgcaa
     3181 taggagcaaa gacaactgct agatagttat agaagaaatc gcctttgaag gcataagcta
     3241 gcgctccaat gatgaaaaat agaacgactg cctgaatcac tgctaataaa attactcgtt
     3301 tcatgtgacc tcctgactct attatagcat gagaatcatc aaaaagccga ctaaattatt
     3361 caaagcgtga agagaaatac tgtagaccag accttttctg ctaatgtaag ccaaacccaa
     3421 actaaaacca aggctaaaat agacaaaaaa ttgttgcaca tcacctggaa aatgaatcaa
     3481 ggcaaataga agactagata ccagaagaaa aatcagggtt cgtttactat tgtcctgctt
     3541 aggaaagaga tagcgtgcta acatccctct aaaaacaatc tcttccgtca aaggagcaaa
     3601 aataaccaca gcaaagaatg agaaaagtgg ttgagacaag gtcaagtctg tcgctatttg
     3661 ctgatttact gaaggatcat ctggcaagaa gaattgaacg accagagata agaaccaaac
     3721 caaggcagga agccaaataa atcggttaaa gccgctcttc tcaatataaa caggagcttt
     3781 ctgataccat ttgtaaatgc cgtacacata tactccagcc aagaccacat agagtagagt
     3841 aacagcatag ggtgaagcgc ctaaagcaag cgacgcagtc gcgagcccct gaataaagcc
     3901 atagataaag aaaaaggata gaagggctag aagaatccag ccaaggtttt taagtaattt
     3961 catagataac tcctttattt gaaataacgt tttaccatag gtaactgcat cacattgata
     4021 taaacatgga tggctcctac aagcaagaaa gctagtaact gaatctctcc tgtcaagaaa
     4081 gaaatgataa taagaaaaat atataaggct ggtaagacat attggtgtaa ttggaataaa
     4141 attcgaaaac tctgttccaa attagcctga cgctcccctt catcataaga atttatatag
     4201 ttcaagacat cctttggtgt agcgaaaaat tccaaatcaa actgacgaac aatcgcaatg
     4261 gttttaaaaa gagatttttg agcgactaag aataccacaa agagtaagaa agaaaggaaa
     4321 aatgtttgag ggtttgtatg caatataatc acctcactta atgaaataaa aatagccaat
     4381 ggaatcgcta cacctgtaat attaaaagca atggttccaa actcaagatt ccgatacatt
     4441 tgcacataat aggtttcatt cagatcgtca tccatttcct cttgatacaa ggaatgaaat
     4501 tttctgcttt tctttaagaa attgaaagtc aaaaacatac taatgaaacc tatcagtaaa
     4561 caaatagctg atatccatgg catcaaggct tttacatcta aaataatttc gtgggattcg
     4621 acacgtgcct taaacatccc tacaaacatg cccaagaacc cccccaagac aatagacatc
     4681 aaaaataaca atctacgttt ctttttcata ttcattctcc tttttcactt gctagatttt
     4741 tggatttctt ttcaatccat tcagttactg ggatgagagc aaagtagacc caaacaaatt
     4801 ggtcgctttg atagggatta aaccagctta ggtccatccc aatcagtaga aatacgctga
     4861 ctaataaagc tatgaccact acataataaa tcactttata cttgttcatc actcgtcctc
     4921 ctccaaacga aataccgatt cgactgtttc gttgaaaatt tgagatattt tcagggcaat
     4981 gataatggat ggggtgtact catcccgttc tagtaggcta atggtctgtc tggaaacccc
     5041 tgccagtttg gctaggtcgg tttgattgag accatcgcga gctcgaagct cttttagacg
     5101 attttttagt tgcatgttac acacctactc tccgtcaaat tcaacggttt ggatatcttc
     5161 aatacgttgc aacttgaatt tttctttccc ctttttatca acactacgta gtttgaccca
     5221 ttcctcgtca acatccacaa cttcccagtt atctggccca atatacactc ccgttataat
     5281 tggttccttt ccaatcattt cttgcaataa tctcgacatt tctgcgtttc ctttctcttt
     5341 tcgctcaagt tttttgattt tattctctag tttcttgatt tttttagaat tattagaata
     5401 aaagaaaatc ataaatagta taaatactag tacccacatt ataactcctt tctgcttcct
     5461 atttcttaac ttgaattcat tgtaacacat ctttttcttt ttgacaatta tagttgtcaa
     5521 aaagtttatg atttttgtca ttttgcaaaa gaaaaaggtc aggagtaagt tcctgaccac
     5581 tttatctatc attaatactc ttctaaaatc tcttcaaacc acgtcagctt caccttgccg
     5641 taggtatagt tactgacttc gtcagtttca tctacaacct caaaaccatg ttttgagctg
     5701 acttcgtcag ttctatccac aacctcaaaa cagtgttttg agcaacctgc ggctagcttc
     5761 ctagtttgct ctttgatttt cattgagtat aaaatcctag tttttcaaag atttctgaga
     5821 agttttggct gattgtctca agtgacactt gcacttcttc tcgggtttgg ttgttcttga
     5881 ccgtcacttg tccgctttcg acttcgctct ctcctagggt gatgagggtc ttagccgcaa
     5941 agacatctgc tgacttgaac tgagctttga gcttacggtt gaggtaatca cgctctgctt
     6001 tgaaaccttg ttggcgaaga gcttggacta gttctagggc tttgacattt gctccatcac
     6061 ccaagactgc gatatagaca tctagggcgt tttcgatagg gagggccacg ccttgttttt
     6121 caaggatgag aagcaggcgc tctacaccaa gcccaaaacc aaatccagca gtttcagggc
     6181 ctccaaagta agcaaccaaa ccatcgtagc gaccacccgc acagactgtc aagtcattgc
     6241 cctcaatctc tgtgataaac tcgaaaatgg tgtggttgta gtagtccaga ccacgcacca
     6301 tattggtatc gatgatgtaa tctactccaa gattttccaa catctgacgc acagcatcaa
     6361 aatgagtttg gctttcttca tcaagaaagt ccaagataga cggcgcattc tctactgcca
     6421 ccttgtcttc tttttcctta gagtccaaga cacgaagagg attttcctcc aagcgacgtt
     6481 ggctatcctt agacaaggtc tccttgagcg gtgtcaaata gtcaatcaag gcttggcggt
     6541 aggctgcacg gctctcagga tttccaagag tgttgaggtg caatttgaca ccttgaatac
     6601 cgatttcctt caaaaaatgg gctgccataa cgattgtttc cacatcggta gctggattgc
     6661 tagagccaaa acactcaaca ccaatctggt ggaattggcg caagcgccct gcctgtggac
     6721 gctcataacg gaacataggt cccatgtagt agaacttgct tggcttttgc acttctgggg
     6781 cgaagagttt attttccaca taggaacgga caacgggcgc agttccttct ggacggaggg
     6841 taatatggcg gtcacccttg tcatagaaat cgtacatttc cttggttacg atatccgttg
     6901 tatctccgac agagcggctg ataacctcgt aatgctcgaa aataggcgta cgcacttctg
     6961 cgtagttgta gcgcttgaaa atctcacggg caaagccctc aacgtactgc cacttagcag
     7021 actcagcagg taaaatatcc tgcgttcctt ttggtttttg taatttcata gggaatcctc
     7081 tttaaactta atagtcttat tttaccataa atagagggat taaaacagtg agaaaaaaat
     7141 taggatttag atatcatttt tgagattaag aattgtcaaa aaaatagcta gcaaggaaag
     7201 accaacaaat agcatccaag tcaactgtat attccatacg gctactagtg aaaaacaagc
     7261 tgttcccaca ggtatggata aggtaaacaa tagatctaaa aaattactag tacgagctag
     7321 aacctctgga gctagatttt tcatgagcat ggcactaatc tttggttgaa ctttaccaga
     7381 cacatacaga gtaaagaaga gaaatagcaa accaagcacg acttgattga ataaattagc
     7441 caaaccaact agactaagtc ctacagtcgc ccacatcatc aatctaggca aggactgctt
     7501 cccaaaataa gcattgcccg taaggctact gatgatgatt gctactaaaa cacagaattg
     7561 attgataaat agtgcctctg tataagaaaa attcaagaga gaatggctca aaaagaagat
     7621 attataaatt ccacccaaag cgccacccaa ggaattaata agcaagacag caaagagcat
     7681 aaaaccaaag tttttctgcc cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt
     7741 taggaactgg tctttgatag aaagcttctc attttttaag ttttcaccat cagcagatga
     7801 cattgacagg ctcaatttgc tttttcctaa aaagaggata gtggctgata ctaggaaaaa
     7861 gcaggcattg attcccgcaa cgagagaaaa attgttgacc gatagagcta agagccagac
     7921 tccgaaagct tgaccaccaa tagctgaaat ataggtgatg aactgtgaaa aagaataagc
     7981 ctccatcaga tcatcttcag ctactttttc cttaataaga ggcatacgca ggccacctgc
     8041 aaaatcactg atgatatcac taatgacatt gatcaaacac aggctagaaa aggcaaagag
     8101 actagcttgc tgaacaacta gggctgctag aaaaaataga accgcctgaa acaaaccgct
     8161 atagaccatc catttgacct tgtccctcgt gtaatctgcc cgaatccctg caaaaactgt
     8221 aaagaaggtc ggaagaatca tgacaatatt cgccatagca acagcaaaag atgcttgtga
     8281 caaggtcgat gcatagacga taaagaccag gttgaaaatc gaagcaccaa aagcattgaa
     8341 gaagcgtgat aaagtcaaaa ttctataagc tggatttcta aacaatagtt tcatagataa
     8401 tctccctcct tgttctaagt tctacatcaa gaatagcttt agtatagcac ttatgaattt
     8461 gcgaaacgat tcttttgaat atatgcaaca aacaaaatga gcaaaaaaac gatagaactt
     8521 ttgtaatgac ctgagagagg gtaacagttc tatcgcttca aattttttct tggtttgcag
     8581 atactcaaca ttcggtcgta tttctatagt attcggcaga tttattacag ccaagcatct
     8641 caaaaatacg gacagcatcc tccatctttt tctggccttc cttgactcta ccttgcttgc
     8701 tatcaaggag accttctgcc cacagataaa caattcggaa ataggtctca ttttccttgt
     8761 agaaatgctc ttcgataaca cgtttaaaat aataggcatt ggtaaattct tcacactcaa
     8821 tactagctaa aaagccattc aatagtatag tatgaaaaag gtttcgattg ccagacattt
     8881 ccattagaaa atcagattta cgtaccattt ctcgtacata tctagtaaaa agagaaacag
     8941 ataaaaatgg agaactgact gaaaataaat tgagttcata gattccccag atctcggtag
     9001 aaaacaaata atcatgaagg acttttcctt cctctgctgt taactctacc ctttcatcta
     9061 tgctcttcat ataagacttg ataataatgg catttagaat atgtttctgt ttgttgtgag
     9121 aatgggcatg cttttgatac tccctgcgat ataagtcctc aagaggtgct atattctttg
     9181 gttccaagac atctgtaatt tcttttctca actcagaatc tgtatcatac tggaaacctc
     9241 tcgccagaaa gaggatctcc tccacactgg cagatatatt ttccagagca aatagaaact
     9301 tttccaccaa aagctcactc tgacctgttt caaaacggga caacatagac ggcgaaaatt
     9361 gtcctccggt tgcttgtctc agtgagatat ttcttgactc tcgtaattgt ctaaagactt
     9421 ttccaatctg ctccatagac ttccccttga ttccgtattt tcttcatttt atcatatttt
     9481 tcagaaaatt catcaaaaac ttgccaaatt gtcagaatta tgagaaaata gaggatattt
     9541 atcacgtgga gggactgcta tgagagacga tatcaaaatc aatgaccgtg ctttggcctt
     9601 gcaagaccaa attatcgaaa aactagagaa agtttttgat acagatgtgg aattggatgt
     9661 ttacaatcta ggtctgattt atgaaatcaa tctggatgaa acggggctct gcaagattgt
     9721 catgaccttc accgatactg cctgtgattg cgccgaaagc ctgcctattg aaatcgtggc
     9781 aggtctgaaa caaatcgagg gtatcgaaga tatcaaggtt gaagttacct ggtcgcctgc
     9841 ttggaaaatc acacgaatca gtcgctatgg ccgtattgcc cttggactac cacctcgtta
     9901 agcaggccaa tcacttttaa agatgaaaaa agcaagccga aaatggcttg ctttttgata
     9961 ctcaatgaaa atcaaagggc aaactagaaa actagccgca ggttgctcaa aacactgttt
    10021 tgaagttatg gatagaactg acgaagtcag ctcaaaatac tgttttgagg ttgtggatag
    10081 aactgacgaa gtcagtaacc atacctacgg caaggcgacg ttgacgtgat ttgaagagat
    10141 tttcgagtat gagtttattt tttacctgac ttgtccatat tccagaagtc tgtcacggct
    10201 ccgcgtgaag cagatgatac gatgtgggca tatttaccga ggacaccacg gctgtaaagt
    10261 ggtggcaagg ttgtttctgc cttgcgtttt tcaagttctt cttcggatac ggccatggaa
    10321 atttctttgg tatcttggtc aaccgtaacg atatcgccgg tacggagata ggcaattggt
    10381 ccaccatcct gagcttcagg agcgatatgt ccaacaacca gaccataagt accaccagag
    10441 aaacgtccgt ccgtcaagag ggccacctta tctccctgac ctttaccaac aatcattgaa
    10501 gaaagtgaca gcatctcagg cataccagga ccacctttag gtccaacaaa acgaacaacg
    10561 actacatcgc catcaatgat ttcatctgtc aggacggcct gaatcgcatc ttcttctgag
    10621 tcaaagacct tagctggccc aacgtgacga cgcactttaa cacctgatac cttggcaact
    10681 gcaccgtcag gagcaaggtt cccgttcaag atgataagcg gaccatccgc acgttttgga
    10741 ttttcaagtg gcatgataac tttttggcct ggagtcaagt ctgcaaagtc agccaagttt
    10801 tcagctacag tcttaccagt acatgtgatg cgatctccgt gaaggaaacc atttgccaac
    10861 aagtacttca taaccgcagg gacaccaccg acttcgtaga ggtcttggaa gacatactga
    10921 ccagatggtt tcaagtcggc caagtgaggc acacgttctt gaatcgtatt gaagtcctca
    10981 agtgacaagt caacatttgc ggcatgggca atggcgagca agtgaagagt ggcgtttgta
    11041 gaaccaccga gagccatcgt tacagtaata gcatcttcaa aggcttcacg agtcaagata
    11101 tctgatggtt tgagaccaag ttccaacatc ttaacaacag cacgtcctgc tgcttcgata
    11161 tcttctttct tatcagctga ttcagctggg tgagaggatg accctggcaa actcatccct
    11221 agaacttcga tagcagttgc catggtatta gcagtataca taccaccaca accaccaggg
    11281 ccagggcagg cattacattc aagacgtttc acgtcctcag ctgtcatgtc accgtggttc
    11341 cattttccga taccttcaaa gacagaaacc aagtcgatat ctttaccatc aagatttccc
    11401 ggtgcaatag ttccaccata ggcgaaaata gctgggatat ccatattagc aatagcaatc
    11461 atagatccag gcatgttctt gtcacagcca ccgatagcga cgaaggcatc cacgttgtga
    11521 ccactcatag ccgcctcgat ggagtccgcg atgatgtcac gagatgttag agagaaacgc
    11581 ataccaggcg ttcccatagc gatcccgtcc gctacggtaa tggttccaaa ctgtacaggc
    11641 caagcgcctg cagatttgac accttcttta gccagtttcc cgaaatcatg caagtgaatg
    11701 ttacatggtg tattttccgc ccaagtcgaa atcactccca caatcgatgt ttcaaagtcc
    11761 ttatctgtca taccagtcgc acgaagcata gcacggttag gtgatttaac catgctgtca
    11821 taaatgctac tgcggtgacg tttatctaat tcagtcatct tatccctccc atttcagttt
    11881 ttactattat agcacaattt aaaggcaatg aacagaataa aattcttgaa ttttcagaaa
    11941 attctatacg catgtgaaat atttaaaatt aaaaacaaca aagcggatta gtgcactttc
    12001 tgatgaccag aatatgcttt ttaatccgct ttctttaaat aacgtactgt aatttttaca
    12061 gaaattcttt cagataagtg tatttaacat ctatcttgca ttataaattt ctagaacctt
    12121 ctcttttata ttcgattcac tcaaaccata ctcattaaga agataatcca ttttccctac
    12181 ttgaccgaat ctttcttgaa cacccatccg atgaattttt gttattccat catcagagaa
    12241 taattcacat aaagcactgc caattccacc tatctgattg tggttttcta cagtaaatat
    12301 agtttttcca cttaacattg tttttatctg ttctggtatc ggtttgattc taaataaatc
    12361 tatcacacct actgaataac ctaatttaga cagttcatct gcaactcgaa tacttggagc
    12421 aaccattatg ccagaagcaa cgattacaag atcttcacca tgtcttaact caatgtagcc
    12481 cttagaaaaa tcttctccac cttgatatac agcctctgga gcttttctaa ttgttcgaat
    12541 atattttagt ccttttaagt ctaatgtctg attcaatatt tcacgaaatt ggatatcatc
    12601 agttgcttca aaaatgattg atttaggaat taaacgtaac aatccaattt cttcaaatgg
    12661 catatgtgtc ccaccattca tttctgcagt tactcctgca tctgatccaa tcacagtggc
    12721 atccaattgt gcgtatccaa gagaaataaa taattgatca aatactcttc gtgaagcaaa
    12781 aggaccaaat gtatgaagat aaggtctaaa cccctgaata gacaagcctg ctgcaaggcc
    12841 gaccatttct gcttccataa tcccaacatt cacataacgg tctccaaagt tcttttcaag
    12901 attattagta gccatcgaac ttgacaaatc ggcttctaag actactatat cagaataact
    12961 ttgattagcc tctaaaagga agtctctata tacatgccgt aattctttcg tacttctcat
    13021 cattctgttt cctccaattc ctgacttaat ctttctacaa ctgaagttaa catttgtttc
    13081 tcctctacag tagggcgaag atgatgattg gatttcattt cttccagctc ttgaacccct
    13141 tgacctttaa tagtatctaa tacaatgcac ttaggtgatg aattatttga ctgttttaat
    13201 tggacaatcc cttcataaat ttctctaata tctgaaccct tgaccctaat ggattcaaat
    13261 ccaaatgctg aaaatttttc tacgaaatca cctggattac aaatatcctt tgtaaaacca
    13321 tctaattgtt ttttgttatc atcaacaaat acaattaagt tggataactg ttgatgagaa
    13381 gcaaactgta tagcctccca acattgtccc tcatttaact caccatctcc aacaatagca
    13441 taagtataaa agggactctt tcttattctc tgaccatatg caagtccagt tgcaacacta
    13501 attccttgtc ctaaagagcc cgttgtcata tctatgcctg gcgttagatt tctatcagga
    13561 tgagacggta atttggttcc atttgtattt aaagaatata agaattcttt gtcaaagaaa
    13621 ccattcaaat agagtgtact gtatagagct ggtcctccat gaccttttga taatatgaaa
    13681 taatctctat ctcgtgctgc aaatatttct ggagtcattg gcattatttc accataaagc
    13741 actgctaaaa cttctacgat agacagactt cctccgtaat gtccgaatcc aagatgattc
    13801 aatgttctaa gagtatttaa tcggatgtta gtcgcaaatt ttcttaaccc atcttctcta
    13861 tttttactta aaatcatccc ttattcctcc tttgcagatg gctttttaat aaaggatact
    13921 ccaaacataa ctgctagaat aagaacaaga ccaatcacaa tacctgcttg tgagccaaat
    13981 tgatttaaca ttcctaaaat aattcctgat agaccaaaat ctgcatctga gaaagttgat
    14041 ccttggaaac caagtcctcc caaaactggc attaaaaaga ctggaagaaa actgattaaa
    14101 ataccttgta aaaatgctcc aatagtggcg ccacgaacac caccagatgc attcccaatg
    14161 acacctgcag tcgctccaca gaagaaatga ggcacaacac ctggtaagat aacaaccgtt
    14221 cctgaagcaa tcataattgc catacttact aaaccaccaa caaaactaga gataaatcca
    14281 attagaactg cattgggtgc ataagtataa acaatcggac aatccaaagc aggttttgaa
    14341 ttaggtacaa gacgctctga aataccttta aaggctggaa caatttcgcc caaaataagg
    14401 cgaacacctg ctaaaataac aaatacccct gctgcaaatt gacctgctaa ttgtaaagca
    14461 taaactagac cacttgtacc actactgatt tctttttcta tatattctga ccctgcaaag
    14521 atagctacaa taatgtaaat aactgccata gataaagtga tactaacagt actatcacgt
    14581 aaaaaagcta aactctttgg aaatttaatg tcctctgttg attttgattt gtcaccgata
    14641 aggctaccag taaaaccact caaccaatat cccaaagaac tgaaatgacc taaagccacc
    14701 ttgtcatttc cagttaattg aaccatatat ttttgcacaa atgctgggga aatactcata
    14761 ataataccga gtgctaatcc tcctagtaag atgagaggca agctagtaaa gccagcaact
    14821 gataaaatga ccgcaatcat acatgccata tatagagtgt ggtgccctgt taaaaaaata
    14881 tatttaaatc gagtaaaacg agcgattaag atattgaaca ccatgcctgc aaacataatc
    14941 attgcagtag ctgagccata tgttgttaaa gctacagcta caattgcttc attattcggc
    15001 acaacgccag ataaatgaaa agcatgctca aacatggtac caaatggatt taaagaattt
    15061 tgtacaattc ctgcaccacc agatacaact aagaaaccaa caaaggtctt aattccacct
    15121 ttaataatat caggtaattt cttcttctga agaactaatc ctaaaattgc aattaaagct
    15181 actaaaatag ctggtgtact aacaatatcc aatatgaact tcatcatgac gctagcctcc
    15241 tatataagtc ctttttcttc acaaagttta gtaattaatt ctcgtagttc atccatatca
    15301 ataatactat ttaagatacg aacatctcca agatgactag ctgaatcagc tagatcacga
    15361 ccaacaatcc aaatatcagc tgcatttgga tctgctccac ctaaatcata atgttcaact
    15421 tctacatccg aaacattcaa atcactcaat acagattcaa tattcatctg taccataaaa
    15481 cttgaaccta atcctgaacc acaagctgta ccaattttta acattatcta atcctcctgt
    15541 ttaattatca ttttaatgtc atcatagttt tttgatgata ttaaagtttg aacatgattt
    15601 ttatctctta aaattgttgt taaatgtgac aaagccttta aatgactctc attatcaatg
    15661 gctgcaatac aaatcaacaa tcttacctct tgttctggat tatccaataa ataaatcggt
    15721 tcttccaaaa ctaacattga cattcctatt tcattcacac cttcatctgg ccgagcgtga
    15781 ggaattgcta ctcccttccc taaattaata aaaggtccaa actcttctac tttttgaatc
    15841 attgcctcag ggtagttctc agttatctta tcttgatcca aaagcggttt agctgctaaa
    15901 cgaatcgcct ccttccatcc taatttttgc gaactaacct gataggtttc tttggtaata
    15961 agttgttcta gcactggtac aatttccttt ctatcatttt tttggtaaag ataattcttt
    16021 aacgccaatc ttaattccaa ttcttgtgta ataattccat atcttttgac aatattcagg
    16081 atttgttcaa tctcaaaatc tccatactct aaattcggaa aatcttttaa cactagttct
    16141 actagttgta ttgcttgctc ttcagtcatc ataaccgaaa ctagataatt tggcttttct
    16201 gtctccacct ttatggtaga aaaaaccata tcatagtcac tactagcttt cacctgtaaa
    16261 tcatcaatct ttgaggttcc tataaactca atttgaggaa ataatgctaa tagattctct
    16321 tttaacatca atgaagaact aacaccatta ggacaaatga ttgctgcttt atagcatttt
    16381 tgaggcaaag tatctgcttt ctttaaataa cctccgaaat ggataacaaa atatgctgtt
    16441 tcactatcag gtatgggatt gtcaatagcg tccatcaagg gcatcaaaga atctttgact
    16501 aattcaaata aatcaggata atgttcttta acatgcaata catattcatt tgaactaggt
    16561 aggccgaact ttaatctata gtaagccggt ataaggtggc ggcgaagatt ttctctcaat
    16621 ccttcccttt gtttaaaatg taacaaagaa atatcttcca ttctacttat aatagcctct
    16681 gttaattgat taaagtaaac cggagcaaca tctacttcac cttcaaagca acttgataat
    16741 aaaactgtga tatagcgata atcatcctca gaaaacaccg tatctataat tcccaaatca
    16801 accactgtat ccaataaaat agtggttata tcttgaataa caggagatac taatgtctct
    16861 gaaagacata ctctttcaac atccctttga tacctacaca gaatgaatac taaaccgaaa
    16921 aggcaaactt ttaattgatt aacaataggt actagttgta gcttctcata ataatcttta
    16981 actacctgat ttatcaaatc ataagttaat gaataccccc aactggataa aacataatcc
    17041 aaaccccaaa tccctatgga ggattccagc aactcactaa ccatttgaaa agctaagcgg
    17101 tgcttattcc actctgaacc gtgtaaagta taaccttttg ctctactgta ccctagctcc
    17161 aaatcattat ctaacataat ctttcttaat gattgaatat cagataaggt tgtattctta
    17221 cttactttca aaaagtcttg gtaatgatta ttcgatataa aatctaatcg gcaaaaagtg
    17281 taaagataga ttaaagctaa gcgagtcgac tttggtaaaa ccaattcatc cgacttaata
    17341 atatctgtca aagactgctt cgtacgattt gataaactat agcgaccttg ttttttatcc
    17401 agcactatcc ctttattagc tagataaggc actaaataat ctattccttc tttgacttcc
    17461 tttataggta agctcacctt aacagataat tcatataatg atagctcaca atgatccatc
    17521 aaagtcatca aaataactag tgctctataa tcaaacattt ctcttccttt ctaactacaa
    17581 ttatagtgta aaaattataa gaataaaaga gtatttgagc acaatataat ctcacctttt
    17641 aaaagaaatt attgggctga aagtttttag tgattatttt caactttcta ttgactttta
    17701 aaaacaaagt gatatcattt agatatcaaa taatgggagg tcattcttat gactgaaaaa
    17761 ctaatcaatt caaaaccaaa tggtgtattc gcattgattc tcattgagtt gacaatcgta
    17821 cttggtgtct ttatatttat aatgggtgtt ggttcggaaa acatttttgg aattattatc
    17881 ggacctttac taatcgtaat tgcaggtcta gctcatgctg gtttaaaagt tgtcaaacct
    17941 caggaagctc tggttctgac actctttggt aattatacag gtaccatcaa agaacctggt
    18001 ttttactttg tcaatccctt cagcgtagca gtcaaccctg caaaccacac tcgacttgga
    18061 caaagtggtg atgttagcac aaaatctcct tttttaggag ctaaatcatc aaatgacaat
    18121 gatgtaaatc ttgaaattgg caagaaacac atttccctca aagtcatgac cttgagcaat
    18181 tctcgtcaaa aaatcaatga ttgcttagga aaccctgtag aaatcggtat cgcggtaact
    18241 tggagagttg tcgataccgc taaggcagtc ttcaacgttg ataactacaa agaatatctt
    18301 tcactgcaat gtgatagcgc cctccgtaat attgtccgca tctatcctta cgatgtgtct
    18361 cctaatgtgg atactacggg tgacgggcaa gcagatgaag gtagtctccg tggctctagc
    18421 gaaattgttg ctaaccgtat tcgtgaagaa atccaaagtc gtgttgagga tgctggcttg
    18481 gaaatccttg aagcacgtat cacttaccta gcttatgctc cagaaattgc tgccgttatg
    18541 cttcaacgcc aacaagcatc tgccattatt gatgcacgta agatgattgt agatggtgct
    18601 gtaggaatgg ttgaaatggc actagaacgg ctcaatgaag gggaattggt agaacttgac
    18661 gaagaacgaa aagctgccat ggtttcaaat ctccttgtcg ttctttgtgg caatcatgat
    18721 gcacaaccaa ttgtcaacac aggaagtctt tactaaagat ggctgaagct aaaaaaaagc
    18781 agatccccct tcgactctca acaaagttat acgctgcact cgcatcatgg gcagaagacg
    18841 actttcgttc agtcaatggc caaatcgaat atctcctcac agagtgtgtc aagcaacgga
    18901 agaaagacgg aaaatacgta tcagaaacca ttgacgaacc atttgagatt gatatttaac
    18961 accttctcct gtctgccgtg tcggatagga gaattttttc atcttttttt gtcaagtaac
    19021 tttactttac aaaaaaaatg tgttatccta gtatggttga tgaaaatcag tagattgaat
    19081 cgaatataaa tacctaaagg agaaatcaaa atggcagtac ctgcacgtcg cacttcaaaa
    19141 gcgaagaaaa acaaacgtcg tacacactac aaagtaacag ctccatctgt aaactttgac
    19201 gaaactactg gagattactc acgttctcac cgtgtatcac ttaaaggata ctacaaagga
    19261 cgtaaaatcg ctaaagctgc atcagctgaa taatagaagg gagataccat gcgcgtaaat
    19321 attacacttg aacacaaaga atctggtgaa cgcttgtacc ttacttctaa aaacaaacgt
    19381 aacactccag accgccttca attgaagaaa tactcaccaa aacttcgcaa acacgttgtg
    19441 tttacagaag tgaagtaagc attcaatacg aatcaataca gaagaaaaac gcttgatcag
    19501 cgttttttct ttttgttaat ttcactatat tgcaagagaa atcattcgaa tgactacatt
    19561 ttttgaaata agaatacgga ttcggacgat taaaagtaag agaaactcaa atgtaagttg
    19621 gggttccttt tttgtatatt tttcaaataa cgcctctaca ctatttgtag caaattcacc
    19681 aactacagtt gcatcttagt taaaataagt tagaatatgt aagtgagtac cagatatacc
    19741 aagacatcgt caccatctaa ggtatattca aaatacaaaa gttgaccaac tagatttctg
    19801 aatatcctta tatatccatt cttaaaattg gtttaaatag cgtagtcttt taaactagtt
    19861 ttgagaatcc aaaaaatctt cctacatatg taagaagatt ttttagttca gaatgattag
    19921 atttagctaa tggataccta tcctacccac tcaccgttag cattgactcg atagccatcg
    19981 ggtgtagtcg tgtttactaa taaatctcct gaattatagg tatagtacca tttaccagaa
    20041 actgtaaacc aaccagtagc cattgaacct gattc
//
LOCUS       DAVLRT010000033        20013 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLRT010000033 DAVLRT010000000
VERSION     DAVLRT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.033"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(103..1734)
                     /gene="rlmD"
                     /locus_tag="VXM85_001158"
     CDS             complement(103..1734)
                     /gene="rlmD"
                     /locus_tag="VXM85_001158"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1345746.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(1808..2782)
                     /locus_tag="VXM85_001159"
     CDS             complement(1808..2782)
                     /locus_tag="VXM85_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1345747.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSAHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(2856..3470)
                     /gene="pmp23"
                     /locus_tag="VXM85_001160"
     CDS             complement(2856..3470)
                     /gene="pmp23"
                     /locus_tag="VXM85_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1345748.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3470..4444)
                     /locus_tag="VXM85_001161"
     CDS             complement(3470..4444)
                     /locus_tag="VXM85_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1345749.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4452..5708)
                     /locus_tag="VXM85_001162"
     CDS             complement(4452..5708)
                     /locus_tag="VXM85_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1345750.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(5770..6201)
                     /locus_tag="VXM85_001163"
     CDS             complement(5770..6201)
                     /locus_tag="VXM85_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1345751.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            complement(6203..6805)
                     /locus_tag="VXM85_001164"
     CDS             complement(6203..6805)
                     /locus_tag="VXM85_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1345752.1"
                     /translation="MMDRIRQELEKGGAVVLPTETVYGLFSKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLSEISFEEA"
     gene            complement(6789..7628)
                     /gene="prmC"
                     /locus_tag="VXM85_001165"
     CDS             complement(6789..7628)
                     /gene="prmC"
                     /locus_tag="VXM85_001165"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1345753.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7628..8707)
                     /gene="prfA"
                     /locus_tag="VXM85_001166"
     CDS             complement(7628..8707)
                     /gene="prfA"
                     /locus_tag="VXM85_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1345754.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(8704..9294)
                     /locus_tag="VXM85_001167"
     CDS             complement(8704..9294)
                     /locus_tag="VXM85_001167"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1345755.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9418..9600
                     /locus_tag="VXM85_001168"
     CDS             9418..9600
                     /locus_tag="VXM85_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1345756.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            9737..11110
                     /gene="mnmE"
                     /locus_tag="VXM85_001169"
     CDS             9737..11110
                     /gene="mnmE"
                     /locus_tag="VXM85_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1345757.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11157..12092)
                     /gene="dapA"
                     /locus_tag="VXM85_001170"
     CDS             complement(11157..12092)
                     /gene="dapA"
                     /locus_tag="VXM85_001170"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1345758.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12148..13224)
                     /locus_tag="VXM85_001171"
     CDS             complement(12148..13224)
                     /locus_tag="VXM85_001171"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1345759.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDMDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(13566..13638)
                     /locus_tag="VXM85_001172"
     tRNA            complement(13566..13638)
                     /locus_tag="VXM85_001172"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13603..13605),aa:Thr,seq:ggt)
     gene            complement(13738..14247)
                     /locus_tag="VXM85_001173"
     CDS             complement(13738..14247)
                     /locus_tag="VXM85_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1345760.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14245..14341)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14370..14801
                     /locus_tag="VXM85_001174"
     CDS             14370..14801
                     /locus_tag="VXM85_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1345761.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            14883..15260
                     /gene="mscL"
                     /locus_tag="VXM85_001175"
     CDS             14883..15260
                     /gene="mscL"
                     /locus_tag="VXM85_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1345762.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15302..16396)
                     /gene="hemH"
                     /locus_tag="VXM85_001176"
     CDS             complement(15302..16396)
                     /gene="hemH"
                     /locus_tag="VXM85_001176"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1345763.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGMRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENIKMPYLIKKMLEKAGKLPKE"
     gene            16550..17773
                     /gene="pepT"
                     /locus_tag="VXM85_001177"
     CDS             16550..17773
                     /gene="pepT"
                     /locus_tag="VXM85_001177"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1345764.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(17809..18141)
                     /locus_tag="VXM85_001178"
     CDS             complement(17809..18141)
                     /locus_tag="VXM85_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345765.1"
                     /translation="MDALLDQLSANSKDKTALLESTKAILVLNHQIQSKASASEETSP
                     ARNAEANGDNTSAENQPNATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTT
                     GNQEKPVE"
     gene            complement(18146..18439)
                     /locus_tag="VXM85_001179"
     CDS             complement(18146..18439)
                     /locus_tag="VXM85_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345766.1"
                     /translation="MSKANQLLERIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHFYLESNNGSDADF"
     gene            18404..18625
                     /locus_tag="VXM85_001180"
     CDS             18404..18625
                     /locus_tag="VXM85_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345767.1"
                     /translation="MDSFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(18762..18971)
                     /locus_tag="VXM85_001181"
                     /pseudo
     CDS             complement(18762..18971)
                     /locus_tag="VXM85_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
     gene            complement(19092..19234)
                     /locus_tag="VXM85_001182"
                     /pseudo
     CDS             complement(19092..19234)
                     /locus_tag="VXM85_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262445.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(19268..19408)
                     /locus_tag="VXM85_001183"
     CDS             complement(19268..19408)
                     /locus_tag="VXM85_001183"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1345768.1"
                     /translation="MLEITLKSPYQFAHILFQSTIVPHGGHYHFIPESDLSAGELAAA
                     KV"
     gene            complement(19452..20012)
                     /locus_tag="VXM85_001184"
                     /pseudo
     CDS             complement(19452..20012)
                     /locus_tag="VXM85_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5760 a         4208 c         3810 g         6235 t
ORIGIN      
        1 ctattttcta attgatacct tgcaagaaag gaataatagc tgttcctata ccaagtagga
       61 atgtaataaa tgcagaaagt catatactgt cattcttttc tattaaatca ttttgaagtg
      121 tctcaaagca tccatgatgg cttcttcttt ttcaggtgta cactgtggaa taatttgttt
      181 atccttttta gacttgttgt aatgttctcg taattctatt ccacatttct tttttatctg
      241 tgcaatatat aatgtcgaaa cttttaattc aaatttattc caaacatatt ctttgatttg
      301 agcatatgtt gctttgctct ccgcacttgt caaattcatc tcatccagct caatttcaac
      361 acttatgtgc ttatcgacat cgagtttgga caaaagtgct accgtctcga cgtgatgcgt
      421 ttgaggaaat agatccaccg gctggacttt cttcaattca tatcccaatt cttggtagag
      481 tttgatatca cgcgccatgg ttgcgacatt gcaggagata taggcgatgc gatcggctcc
      541 tgtttgggcg cttgctttga taaagctttc tgtcaagccc ttgcgtggag gatcaaccaa
      601 gataacggtt ggttgaatcc catctttaag ccaattcttc atagcatttt cagctgtgtc
      661 acagacatag tgggcgtttg aaatattgtt cagctgagca ttttttttac tattctcaac
      721 cgcttctgga atcagttcaa caccgtagac ttctttgaca tgcttggcga ctgataaacc
      781 aatggttcca ataccagaat aggcatcaat aatcacgtca tcttttttta actctgcaaa
      841 gtcaatggct gtttgataga gtttctccgc catttcagta ttgacttggt aaaaggctgg
      901 gccagcgatt tggaagtcat ttcccaacat ctggtccgta atatagtctt gaccataaag
      961 agtgcgccac tccttaccaa aaatcgcatt ggtattctgg tcgttgatat tttgcatgac
     1021 agacacaatc tctgggaact gcttgataac ttgttcaatc aattggtcaa cacgaaaaac
     1081 ttttggacga gttgtcacca aaacgaccat gatttgtcct gaatagtgac cacgacgcac
     1141 cacaagattc cgaatcaatc cagactgttc cttttcgtca taaggtttta aatcaaaacg
     1201 acggagcagg tctcgaagag ctactacgac ttggtcaatg acaggatcct ggataaagaa
     1261 atcttcaagg ggcatgaggt tgtgcgaatt cttacggaaa aatcctgttt ccaagacacc
     1321 attcactcga cgaacgggca cctgcgcctt attgcgatac ttgactggat gttccatacc
     1381 aagcgtttca gcaacttcta catctgcaat tccagcaatc ttgtagagac tgtccttgac
     1441 ttgcttggtt ttaaacttga gctgttctgg ataagaaagg tgtcctaaat ccgcgattcc
     1501 tgaacgcagg taagccaaat ctagatcttg attacggtgt ggtgactgga caaggtattt
     1561 ttcaactttt ccaaagccaa tctttttatt gaccttgagg acacgcatga gaattttttc
     1621 actcggtaaa gcattctcta caaaaaagac caaaccatct accttggcaa ctcctgcccc
     1681 ttcatgggtc aagtcaacaa tttcaacttc tacaatatca tttttcttta acattctttt
     1741 ctctttctat tggccgacaa aaaagacggc aacgttacgg ttaccatcca ttataccatg
     1801 aaatttctta agaaccaaaa ctcttgaaga agttgacaag ggcttgccag agggagctta
     1861 agaagttacc accgtcctct agcgccttat ccgcatcaaa gttaaggttg atatttttaa
     1921 aactgtcgcc agcttgtgat acgatgcttt gtttaaggtc atttagggtt ttagtgaaat
     1981 ctgcattgct gaggatatca ctctttgaga gattcaaggc aaaattgatg atgatattga
     2041 tctggtttcc tgttatgacc tgatcaagtt tgtaattttt taaggtatct tcaacaatct
     2101 tgcgtacatc ttcttctgtc agatttccct tgctttcttt agctttggcg agtcctgact
     2161 tgatatcagc tagggcaacg tttaatttat tagcatcata gcctgatttg tccttgtttt
     2221 cagcattgat atctgacaaa gcctttagct cttcttgagc caaatcttta ttggcttgcg
     2281 gcaccttggc tccattagct tctagcgaat agtagatccc agccaaagca ctctcacctg
     2341 taactggaat aggggctgct acagtgattt tggcatgttc catacccagc gttactgctg
     2401 cgtttcggta catatcctga gtcaccttag tgatattttc tggtgtttca atcttgacct
     2461 caagtggcga tttgtcacct agcttttgaa tcttggctga tgaatacaac tgtaagctag
     2521 agtcattggc cacattcatg attttagaat aaacatcagg tgtcatggtc ttgagttctt
     2581 tggtatctgt tgaggcattg tagcccagtt ttttaagagt ttgatttttt tggtcttcag
     2641 atagggagga acctaggaca tattcaggtt ggacataggt ttcatcgata actttttgaa
     2701 catctgttgc tgcatgggcg ctattcatag ctgttactgc ccacaagacc gcagcactgg
     2761 tcagaaagag tttctttctc atagggaatt tcctccttta cttttctaga gtaatatatc
     2821 tatcttaaag aaaacttata acaaaaacac ctggtctagc cagatgttga aaagagagtg
     2881 aaacatttga tgatgtaaag gttaagtcgt acctgtctag aataataata gtttcctcca
     2941 tttacataga gttcagcacc gtgaaaaatg gaaatggggt gaatataact ataagtcttt
     3001 ccagtcgtat taccaagcaa gggggcaaca gtctcacgag agtactgttt ggctagagcc
     3061 aaggtatttt ccttgccatt ttgggcgata aaatcgatat aggcaggtcc aaaattatag
     3121 gcttgaacag ctgtccagat atctacaccc ttcttctgcg ccagatagag attgcctgtc
     3181 agagtttgaa tgccttgccg aatgctagag gcattatcat tgatggtatt ggtggaacca
     3241 cttgcagact cactagactg cataacatcg ccttcttttc cttttgtttc agtataaatc
     3301 atagcaagca caagctcttc gtttgctggg gtgtcttttt cactcaatat ttctcgcacc
     3361 atgggttgat aggtcatgac ttgtttgaca tcttgatgaa cgcggtaagc tttatatcca
     3421 gcaaaaagga agactgctag tacaagcact cttcgaattc gtttaaacat tatttacttt
     3481 ggatatcctc gatatttttg attaagatag agtaggttcc attttcgttt tggataaact
     3541 caacagactc ggcgtcttga tagacgttat tgggaacgat gagctcaatt ccatttgata
     3601 aggagagttt ttggttttca aatttcttta attggcgact ggcatcaatt tcatcaaatt
     3661 gaacaggttc tggtacggct tctttgactt ggtcaataaa gctcaaacga gccgtcagat
     3721 tgttgtcaaa aaggtcatta gccaatttct caggtgacaa ttcattgctt tcttctaggt
     3781 tgttgaaaat agctgatttg accttggatt gaaattgaaa atcatctgtg ttaaaagatt
     3841 cagcaattct ctgggctgtt ttttccagtt ccttgataga ttttttagga gaaatcttag
     3901 gagcgacagc aagaagatta tctgaaaaat agttcaaaaa agtcccgttg tacttgattc
     3961 gtttttcaat caggtgatac ttgctactct gaagattgac caccaaggcc tcatcagctc
     4021 ctgttccaaa tccaggcagg ttattctgag ttagcttgat tggattatca acttctcctc
     4081 cgaggtgggt caaggtctcc cgcagggcaa ttcgcaagaa agcgaaatgt tctacacctt
     4141 ctttagaaaa ttgaacaaaa atcaagtcat tggtcttgag attttcagaa atgctaaact
     4201 cctctttcca gagattagcc agcgttactg atgtctccaa caaatcgtct gtaatatgat
     4261 tgaagaaggg attttcttct tcgaaaatcc cagtcttggc ttcatctgaa tacacatgtt
     4321 caattttttt acgtaggtat tcttcgattt ttggagtaat attgagaaac ttatctgcta
     4381 agaacagctc ggtatcatcc ggactgaact ggtgaataat ggctttctta atataaatgt
     4441 ccataaaagt tttattcctc gtataatggg aaggcatctg tcaattcttt gactgcactt
     4501 ctcacttctt ctaatacagc ctcattttct gaattcttaa gggttttaat gatgagttca
     4561 gccactttgc gactttcttc ttcaccaaat ccacgtgcag taatagctgc tgctccgata
     4621 cgaatcccac ttgtcttgaa tggtgacaag ctttcgtaag ggattgagtt tttatttaag
     4681 gtaatattga cttcatccaa caagttttga gcaactttgc cgttttctac aactttagtc
     4741 acatcaacaa ggaagagatg gttttcagtt ccacctgaaa taatacggaa atcagggtct
     4801 tgcaagaaga catctgccat agccttgctg ttcttaatta cattggcagc atattccttg
     4861 aaggctggat ccaaaacttc tttgaaggaa actgccttag ccgccacaac atgctctaaa
     4921 ggaccgccct gaatacctgg gaaaatagct gaattgattt ttttagcaag ttcttcgtca
     4981 ttggtcaaaa tcaaaccacc acgaggtcca cgaagggttt tgtgggtcgt tgttgttgtg
     5041 atatgagcgt atggaactgg gcttggatga aggccagccg caaccaagcc agcgatatgg
     5101 gccatgtcca ccatgagctt cgcaccgaca gcatctgcga tttcacggaa ttttgaaaaa
     5161 tcgataattt gagaataggc tgaagcacca gctacaatca gttttggttt tacttcttgg
     5221 gcttgtttca agatagcatc aaagtctaag agttccgttt taggatcaac actataagaa
     5281 acaaagttgt aggtttgacc agagaagcta acaggagccc catgagtcaa atgaccacct
     5341 gatgccaaat ccattcccat aaccgtatca cctggctcaa tcaaggacat gtaagccgca
     5401 cagttagctt ggcttcctga atgtggttga acattggcaa atttagcacc gaaaatttct
     5461 tttgcgcgtt caatagcaag agtctctaca acgtctacta catcagttcc accataataa
     5521 cggcgtcctg ggtaaccctc ggcatattta tttgtcaaga tagacccttg agctgccata
     5581 acagccttgg aaactacgtt ttccgaagca attaactcga tattattttg ttggcgttct
     5641 tcttctttgg caatagcatt ccagagatca gcatcatatg ctttaaaatc atctttgtca
     5701 aaaatcatag gtcttctcct ttattgtgtg actagtccat tagtttgatt ttacaataag
     5761 aaaatcaaac taacagatgc gaataaaccg tttctgcatt ttatcacaag tatagccaac
     5821 tttttcataa aatgcatgag cacccagacg atgattggca gaatttaagc ggataaaccc
     5881 ataaccacat ctttttgctt cttcttccaa cccttgtagt aaacttttac caataccttg
     5941 accttgcgct tgaggtgaaa ctgctaaagc taagatatta aatcctgctt tggaatagag
     6001 tgattcgtaa acttcagcgt ggacatatcc aagtaagaca tgattagctg catcctcata
     6061 gccaagtagg aaatgatggg aatcctgaga cagtctagct agttggctag ccgtttcctc
     6121 tggactaaaa gtataaccca aagcctcttg gttgatgtca catatcgctt tcacatctgt
     6181 ttcttgcaaa tctcttagca tttcaagcct cctcaaaaga aatctctgac aaccgagcaa
     6241 gaatatcttc tcgtttaatt gcgccttggc gtaagatttt caccttgtct ccagacaaat
     6301 ccacaatagt tgaatcctgt ccagttagaa aagcatcgtc ttccagaccc agaacctctt
     6361 ggtcaaaatc cttcagaatt tgttcaaagg ttacaccact tgcctgacct gagatattgg
     6421 cagacggccc aatcaaggga cctgtctctc gaattaaatc cagtgtgata gggtgactgg
     6481 gcatccgaaa tccaatagtt gcaaggtcag aatttaccca atagggaact cggtcattgg
     6541 cttcgagaat aatggtcaag ggacctggca aaaaggtctc tacaagtttt tgtagataag
     6601 ctggctgatt ctttgaaaag tgcaagatgt cctcgaaaga ggcgatattg agattgagtg
     6661 ccttatctct aggacgacgt ttgagttggt aaacatggtc aactgctttt tcatctaagg
     6721 ccttggaaaa aagaccataa acagtctctg taggtagaac gacagctcca cccttttcca
     6781 actcttgtct aatcctgtcc atcatcaacc acaaccatcc tattttgacc aaattggtct
     6841 ttgagtgttc gaactctttt ttcaggaaga tgttttctaa aaagttcggg aacactttga
     6901 ccttgcttgt atccaatttc aaggtaaatc ttaccaccat ccttgagata gtcttttgca
     6961 tcttccgcaa ttctacagta aatagctagg ccatcctcgt ctgcaaagag agctagatga
     7021 ggctccgaat gcaaaacatt caagcctacc tctgactcat cttcacgaga gatatagggt
     7081 ggattggata caattatatc atatttttca gaaatttctg caaaacaatc agatttttta
     7141 aaaaatatat taagattttg atcgctagca ttctctgatg caagctctaa ggcctcttga
     7201 gaaacatctg ctgctgtcac tgaccaatct ggtctatttt ttgctagagc gagagcaata
     7261 gctccactac ctgttccaat atccagaact gagagattcg tctcaagatt ttcagttaga
     7321 ataagctcca ctaactcctc tgtttctgga cgaggaatca aaacccgttc atccaccttt
     7381 aaatgcattc cataaaaatc tgcctgtcca atgatgtact gagctggctt gtgagctgct
     7441 agttgctggt aaatatcttc tacaaattgt ttttcttcct ctgttgtcac ctcctgctgg
     7501 agggcaaaaa taaagtctgt aaaagataga tttttcagac tacgatagac aaaagagagg
     7561 ctttccgctt cctctccttg tcttatcaac tcttcttcaa aatttgaaaa taattgagct
     7621 aatttcatta tttgtttaat tcttctagtt tttgtgtttg gtcataaagc accaaggcat
     7681 ccacaacttc gtccaattta ccagacaaaa tcgtatctag tttttggagg gtcaagccga
     7741 tacggtggtc tgtgacacgg ttttgtggga agttataagt tcggatccgt tctgaacggt
     7801 caccagtacc gattgtcgac ttacgctcag cgtcttgttc atcctgagca atctgagcaa
     7861 agtggtcagc gacgcgtgca cggataatct tcatagcctt ctcgcggttc ttctgctggg
     7921 tacgttcttc ctgcatctca accttgatat tggttggcaa gtgaacgata cgaacggcag
     7981 tcgcaacctt attgacgttc tgtccaccag caccagaggc gtgatagatg tcgacacgaa
     8041 ggtcttttgg atcaatgtcg tattcaacct cttcaacttc tggcataaca agaactgtcg
     8101 ctgtcgaagt atgaacacgg ccttggcttt ctgtcacagg aacacgttgc acacggtggg
     8161 cacctgattc atacttaagc ttagagtata cagactgacc tgaaaccata gcaaccactt
     8221 ctttaaaacc accgacacca ttcatagagg cttccatgac ttcaaagcgc caaccttggg
     8281 cttccgcata cttttggtac atagttagca aatctccagc gaaaagtgcc gcttcgtctc
     8341 caccagctgc tccacggatt tcaaggatga tattcttgtc atcgtttgga tcctttggaa
     8401 ggagcaaaat tttcagtttt tcttcatatt cttctttttc agccttggca tctttgagtt
     8461 cttgcttggc caattcttcc aagtccgcat ctccgcctga ttccttaatc atctcttcgg
     8521 catcgacgat attttgaagg acttgtttat actcacggta ggctattacg gtgtcacgat
     8581 tggaagcttc ttcttttgaa agctccataa aacgcttggt gtctgaaacg acatcagggt
     8641 cactcagcaa ttctcctagt tcttcataac ggtcttctac agcttgtagt tgatcataga
     8701 tgttcatttt tcttcattct ccttattgat ttcaggggca aaataatgtt tacggcaaac
     8761 cgagatatag gtttcattac caccaatctg gatctgttct ccatcgtaaa cgggcagtcc
     8821 atcctgtgtt cgcaacacca tggtcgcctt tttcttgcaa tactgacaga tggtcttgat
     8881 ttcgtcaatc ttgtccgcta aaagcaagag atatttggaa ccttcgaaca attcattgcg
     8941 aaagtcattt ttcaagccaa aagccatgac gggtatgtct aactcgtcca caacacgagc
     9001 taggtcataa acatggtggc gtttgagaaa ctgggcttca tcgaccaaaa cacagtaagg
     9061 tttttctggt aggtctcgga tatagccaaa gatatccgtt gtttcctcaa tcgcaagggc
     9121 agggcgtttc atgccaattc gactcgacac atagccaaca ccgtcacgcg tatccagagc
     9181 cgaggtcata atgacaacac cttttccttg ctcctcgtag ttataggcca ctttgagaat
     9241 ctcaatcgtt ttaccagagt tcatggtccc ataacgatag tacaactgtg ccatgtttct
     9301 tgcttcacgt ccatttctaa atttttgcta cattctagta tatcataatt ttcttaagct
     9361 ttaaacggca aaatgtggta aaatagaaga aatcaaaaac tagtggagga agctattatg
     9421 ccatttgtac gcatcgattt atttgaagga cgcacgctcg agcaaaagaa agctcttgct
     9481 aaggaagtaa cggaagcggt tgttcgcaac actggagcac ctcaatctgc tgtccatgtc
     9541 atcatcaacg acatgccaga aggaacttac ttcccacaag gggaaatgcg taccaaataa
     9601 gctagcttaa gcagaattgc ttaggctttt tcaatctcca agtagcattc attgaagaaa
     9661 tatcctaaat ttgttacaat ttgaaaagaa acttggagaa tttccaagaa aagagcaatt
     9721 aattaaagga aacattatga ttacacgtga atttgatacc atcgctgcta tatctactcc
     9781 actaggtgaa ggggctattg gtattgtccg cctgagcgga acagacagtt ttgctattgc
     9841 gcaaaagatt tttaaaggaa aagacttgaa caaggttgcc agccacactc tcaactacgg
     9901 tcacattatc gatcctctga ctggtaaagt catggacgag gttatggttg gggctatgaa
     9961 gtctccaaag accttcactc gtgaggatat tatcgagatt aacacccacg gtgggattgc
    10021 ggtgaccaat gagattctcc agctagctat ccgtgaggga gctcggttgg cagaacctgg
    10081 tgaatttacc aaacgtgcct ttttaaacgg tcgcgtagat ttgacacagg ccgaggcggt
    10141 gatggatatc atccgcgcca agacagacaa ggccatgaac attgcagtta agcaattaga
    10201 cggctccctt tctgacctca ttaacaatac ccgtcaagaa atcctcaata cacttgccca
    10261 agttgaggtc aatatcgatt atcctgaata tgatgatgtt gaggaagcta ctactgccgt
    10321 tgtccgtgag aagactatgg agtttgagca attgctaacc aagctcctta ggacagcccg
    10381 tcgtggtaaa atccttcgtg aaggaatttc aacggctatc attggacgtc ccaacgttgg
    10441 gaaatcaagc cttctcaaca acctcttgcg tgaggacaag gctatcgtaa cagatatcgc
    10501 tgggacaaca cgagatgtca tcgaagagta cgtcaacatc aatggtgtac ctctcaaatt
    10561 gattgataca gccggtattc gtgaaacaga tgacatagtg gaacaaatcg gtgttgagcg
    10621 ttcgaaaaaa gccctcaagg aagccgactt ggttctacta gtgctaaatg ccagtgaacc
    10681 actgactgcg caagacagac aacttcttga aattagccaa gataccaatc gcattattct
    10741 acttaataaa accgacctgc cagaaacgat tgaaacttcg aaactacctg aagacgttat
    10801 ccgtatttca gtccttaaaa accaaaacat cgacaagatt gaagagcgaa tcaacaacct
    10861 cttctttgaa aatgctggct tggtcgagca agatgctact tacttgtcaa acgcccgtca
    10921 catttccctg attgaaaaag cagttgaaag cctacaagcc gttaatcaag gtcttgagct
    10981 ggggatgcca gttgatttgc ttcaagttga cttgactcgt acttgggaaa tcctcggaga
    11041 aatcactggg gatgctgctc cagatgaact catcacccaa ctctttagcc aattctgttt
    11101 aggaaaataa gaaaaatcca tgatccttca ttcggtcatg gattttattg tctttattag
    11161 taatctggtc ttaagacccc tgttacagtt gccttagttg cttcgtagtc gccatctacg
    11221 acaaccttga taatgcgttt ggcatcttct tctggtgctg gaacaagagg tagacgagtg
    11281 ggtccagctt caaatcccat atagttaaga actgccttaa ctggagcagg acttggataa
    11341 gagaagagag cattaacctt aggaatgaat ttacgctgaa ttgctgcggc tttcttcata
    11401 tcgctttctg caatggcagt aaacatctcg tgcatttcat ccccatttgt atgagaggca
    11461 acagaaataa ccccatccgc cccaaggttc atggcatgga aagcatctcc atcctcacct
    11521 gtataaatca agaactcttc aggcttgtgc tcaatcaagt aagccatatt agccaagcta
    11581 gtacattctt tgacaccgat aatatttgga tggtcagcca aacgaagcat ggtttctgga
    11641 gtcaattcga caactacacg ccctggaatg ttatagataa taattggtag gtcagaagca
    11701 tctgcaatag ccttaaagtg ctgatacatc ccttcttgag aaggtttgtt gtagtaagga
    11761 acaatagcaa gcccagctgc gaaaccacca aattccgcta cttctttgac aaactcaata
    11821 gagtcacgcg tatcattagt acctacaccc gcaatcaaag gaacgcgtcc attgacaacc
    11881 ttttgtacag ccgcaaacaa ctccaactcc tcatcgtggg tcaaagttgg actctcagca
    11941 gtcgttcctg cgagaagaat tccatccgta tgatgggcca ataaatgctc aatcaaggct
    12001 ggaatagcat caaagttaat ggaaccatcc tcatggaagg gggtaataaa ggctgtaatg
    12061 attttacatt cttttaaatc ttgataagac acgaaaacac ctctctattt caaagaagga
    12121 gttcatctga actcctaaac gatatgacta ttttaattca aatttcaatt cggctgttgg
    12181 acgaaccaat ccacgttcat gaagagtttc agcaatctga actgagttcc aagcagcacc
    12241 tttgagaagg ttatctgaaa caacccacat gtgaattcct ttttctgcat ccatgtcttt
    12301 acggatacga ccaacaaagg tatcacgcga accaactgca ttgatagctt gaggatagat
    12361 ttgatgagct acatcatctt caagaacagc acctgggaag gctgcgatag ctgcttttac
    12421 ttcttcgatt ggagccactt cttttgtttc gatataaaca gactcagagt gagctgacaa
    12481 gactggaata cgcacacatg ttgcagatac tgcaatgcta tcatcttcca taattttctt
    12541 agtttccttg gtcatcttca tctcttcgta cgtgtaatca ttatcagtga aaacatcaat
    12601 ttgtggaaga gcgttaaagg cgataggata atgtttcttg tcaccacctg aaggcaagat
    12661 ttccgcatgc aaatcacgtg gtttcacacc atcattcaag acttcacgaa gttcacgttg
    12721 tgtctcaaga attgctccca taccagcacc tgaaacggct tgataagttg aaacaatgat
    12781 acggtccaag ccccattttt ggcgaaccgg ctcaagagcc accatcattt ggattgttga
    12841 acaattaggg caggcaatga ttccgttgtg agcatcaagt gcatgagcat tgacctctgg
    12901 aacaaccaaa ggaacatctg gattttgacg gaaataagat gtattatcta ctactaccac
    12961 gccagctttt actgcgtatg gtgcatactt agctgatgta gaactacctg ctgaaaagag
    13021 agcaatatca actccttcaa aagctgtttc agtcgtttct tcaattgtaa tatcttgatc
    13081 tttaaatttc aatgacttac ctgctgaacg tgcagaagca aggtaacgga ttttatcgat
    13141 tggaagtgtt gattcttcca acatttttat catctgagca ccgacagcac ctgtcgcgcc
    13201 gactacagca actgtatatc ccataaataa cctctttcgg aattttctaa aaatttctat
    13261 aatagaagta ttatactact ttttccatga attgcaaagc tttttcatta ttttttggaa
    13321 aataaaaatc ccagtcgcta gactagggac taagaggcat cttcatgtga tgagcaggtt
    13381 cacacaactc atcaaggtcc gctcctgcgt tatgacctcc ttatgctcaa tagtagtccg
    13441 aagactacct atcgactcat cgcatattct atattactag agatacttga ttttgtcaat
    13501 aagaaatttt ttcgatcgag tataacaaaa aactcttgta ttcaagagtt tccctataca
    13561 tatcatgcta attgccggga ttgaaccggc gacctcatcc ttaccatgga tgcgctctgc
    13621 caactgagct aaatcagctt acttaagaag tatactataa tcagaaaaac ttgtcaagtt
    13681 tccttagaaa ttttccatag gaaaaagcca ggtaagaact acctagctta tcttcttcta
    13741 tttacttaaa tcaattcgac gaccaatttt accaataata atcgctccaa taatcaatga
    13801 agcaaattca ccgattcctg ttgtaaacca agtaaggaag aatggtaatt ctgctacaat
    13861 atttaactct gcagcgatag taaacatgga aattgaaaag aggattgaaa agaagaaatg
    13921 atcttttcga atcaatccgt taaagaggta atccttgcta tattttgcaa aaagccaaac
    13981 acctaaactg aggaatacta aggttgaacc accaccaaca aagacatcta gcagtccgaa
    14041 gctaaagaaa ttagcaatca tacaaccgat ggtaactccg atgatgtact taggattgta
    14101 aaaagccata aagttcatca tttctgaaat acgaaactga taagcaccat agctgatggc
    14161 atttaggggc ggtgtgacag tcaaaacgac gtagatagca gcgacgattg caatatctgc
    14221 aacatcacga atagttaatt ttttcatctt ttctcctttg gcggttatcc gcgtgtaata
    14281 tgcttggtga aagaagctaa gcaccaaggg ttgattcatt caaccttact agtatagcac
    14341 aatagcctgc tttatgctat acttaaagca tgaaaaaacc tattcaaaaa ttttttaaca
    14401 atgaaatctt agcctatctc ttttttggcc ttgcgacaac tctagtttcg attctctcac
    14461 gactagtcat ttatcaacta agtcatcagg aactccttgc tacggccttg gcaaatatta
    14521 tcggtatctt atttgccttt atcacaaatg atacgattgt atttaagcaa aaaaagaaaa
    14581 atagacttat ccgtttagtg aaattttctc ttgctcgcct ttctactttt ctgttagact
    14641 tgtttttaac ttttctcttt gtaacccaat ttcctcacat catagggcaa ttcgtaactg
    14701 aaaacattga taagataaat gctattgaaa caataattgc acaaatattc attattatca
    14761 tcaattatat cttgagcaag gtctatattt ttagaaaata atgctccatt tatgagcctt
    14821 ttaattgcct tctataatga tttaggatac aatgaaacac gaaacattga aaggaaaaca
    14881 gtatgttaaa aaatctaaaa tcgttcttgc ttcgaggaaa tgttattgac cttgctgtcg
    14941 gtgttgtcat tgcctctgct tttggtgcta tcgttacttc acttgtaaac gacattatca
    15001 ctcctcttat tttaaatccc gctttgaaag ctgctaaagt cgaacgtatc gctcaacttt
    15061 cttggcatgg agtcggctat ggtaactttt taagtgctat tatcaatttt atctttgtag
    15121 gtaccgccct cttctttatt atcaagggca ttgaaaaagc acagaaactg actggcataa
    15181 agaaagaaaa aactgccgaa aaaaaaccaa ccgaattgga agtccttcaa gaaataaaag
    15241 ctctccttga gaaaaaataa tcgattaaaa ggatctagcg caaagctaaa tccttttttc
    15301 tttactcttt gggtaacttg cctgcttttt caagcatttt tttgataaga taaggcatct
    15361 taatattctc ccgccctttt ttctcaatca gttttttcac aaattctggc atttgtaaat
    15421 cttcagattc agctaaaatg tttttcgtct cgtctgaagg aactaactca tcaaaggtct
    15481 cttcttctgg gagaaattcc ttaaactctt gattttcatt gactctaact gtattgacca
    15541 aagcatcaat caaacgagaa tctgtatttg gcattggtgg acgatggtag tttaccccaa
    15601 attcctgaca taaatcataa cattccacat cattgtcaaa caagacctca atatgctcac
    15661 tgatgaaact aataggtaca aaaatataat ggtctggatg ttctgtctgt tctctgagat
    15721 actccaaaac atctggttta atccatggaa taccaatatc actctcactc tgccaagtgt
    15781 tggtatattg ttctgaactc aagcccagtt tttcagcgac tagcttgcta ttttcgaaaa
    15841 tttggtctat ataaggatct ccaaagtcca aggcaaaaat aggcacacta tgggctgaaa
    15901 agatgacttt aaagctgtct tgctttactt cttcttttaa aatcttagca atttcatctg
    15961 cccaatagtt taagagcgct tcttcctgat accagtcctt aatgaccaaa aattgaattt
    16021 gtttgctttc taaaaatttt tcatagccca tgacagagta gaaagaataa tggggctcca
    16081 aaatcaagca aatacactgt tcaattccgt ctgcttccat ttgaccgatc acatctggaa
    16141 taaagggact ggaaaactta ttagcaaagt agacactgta ttcattcccc agcctagctt
    16201 ctaccaaggc aacctcttga cgggtaattt tttgcagagg cgtgcctcca attcgtacat
    16261 aattatcata gagagtctga atctcgtggt cttggggtct catcccacga cgaatatttg
    16321 tgaaaaaatc agctacacct tcaaaggtaa tctcttctgg tgaaccgaag gtcatcatta
    16381 aaattgcttt tttcatgact gcttccttgt gatgttttta tcaagtttat tttatcatta
    16441 attcatcgat aagtaaacgc tagcataggg acattctcaa tcttctgtct tttgtttttc
    16501 tcttctttct atgatacaat ggaaaaaata aattcaaaag gagtttttta tgacttatcc
    16561 aaatctcttg gaccgcttct taacctacgt taaggtcaac acgcgctctg atgaacactc
    16621 tactactact ccaagtacac agagtcaggt tgacttcgca acaaatgtcc taattcctga
    16681 aatgaaacgt gttggactgc aaaatgttta ctatctaccg aatggttttg ctattggaac
    16741 cttgccagcc aacgatccgt ctttaacacg taagattggt tttatatcgc acatggatac
    16801 tgctgatttt aatgctgaag gagtcaatcc acaggtaatt gaaaactacg atggtggtgt
    16861 gattgaacta gggaattctg gtttcaaact cgatccagct gacttcaaga gtcttgaaaa
    16921 atatccagga caaacgctca tcacaacaga tggaacaagc ttgctaggcg ctgatgacaa
    16981 atcaggaatt gctgaaatta tgacagccat tgaatatcta actgctcatc ctgaaattaa
    17041 gcactgtgag attcgtgttg gttttggtcc agatgaagaa atcggtgttg gtgccaataa
    17101 atttgatgca gaagattttg atgtggattt tgcctacact gttgatggtg gtccactagg
    17161 tgaacttcag tacgagactt tctcagccgc tggtgctgaa ttgcatttcc aaggccgtaa
    17221 tgtccaccct ggtactgcca aagggcagat ggtcaatgcc cttcagctag caattgattt
    17281 tcataatcaa cttccagaaa atgaccgacc tgagttaact gaaggttacc aaggttttta
    17341 ccatctaatg gatgtgacag gtagtgttga ggaggcgcgt gcaagctaca tcattcgtga
    17401 ttttgaaaaa gatgcctttg aagcgcgtaa agcatccatg caatctatcg ctgataagat
    17461 gaatgaagaa cttgggagcg accgtgtcac tctcaacttg acagaccagt actacaatat
    17521 gaaagaagtc attgaaaaag atatgactcc aattaccatt gctaaagccg ttatggaaga
    17581 tctaggtatc acgcctatta tcgaaccaat ccggggtgga acagacggct ctaagatttc
    17641 ctttatggga atcccaactc cgaatatctt tgcaggtggc gaaaatatgc acggacgttt
    17701 tgaatacgtt agccttcaga ctatggaacg tgcagttgat accatcattg gcatcgtagc
    17761 ttataaaggc taaaaagacg aggtagctca gctacttcgc ctttcttttt attctactgg
    17821 tttttcttga tttccagtag ttgtagaaga ttctgttgtt ttattttctg aagttgattc
    17881 agcaggttta gaatctcttg tattgcttgg tttgttttcg tcgctagcag tttcaatgtt
    17941 agattctgca gttgcgtttg gttggttctc agcactggtg ttatcaccat ttgcttcagc
    18001 atttcttgct ggacttgttt cttcacttgc gctagctttt gactggattt gatgattcaa
    18061 aactagaata gcttttgtcg attcaagtaa agctgttttg tctttactat tagcagaaag
    18121 ttgatctaat aatgcatcca ccttatcaaa agtccgcatc agatccatta ttactttcta
    18181 aataaaagtg aagcgacatg agaatatcgt agagtttttg atagagtaca agtgtctgag
    18241 gatcttgctc agcattttcc ttttcttgtt gaagggcgct agcgatacga gtcaagacat
    18301 cttttacctg actgtttact tcatccaagt ctgcatcagc cttgtttgtg gcagctttta
    18361 gattttctac ttcttctgcc aaagattgtc tgattccttc ttcatggatt cgttccaaga
    18421 gttgatttgc cttgctcaaa agactttcta cttcttcctt gctatctgtc gcagattatt
    18481 ggttgctatc taccatgtac tcctaaaaca ggagagttat aatccaagat tacaaggcct
    18541 tacagaaata agaaatccag ataagacaat gttcgtccaa gacgctattc gcttcgcaca
    18601 gcagcacgga ttcaatatgc tttaatttta aagtttaggt gtcaagacct ctttttagtg
    18661 tgcccaaaat ttagagaagt aatcaatcaa ctaactttta tttttttcaa actttcagta
    18721 aactgaccta aagctaactc aatctgtctt tgttcgatag gcttgtcttt gtagatgctt
    18781 ctgctatcag ctagaagttg atctactttt gccaagactg ccttctcatc aaaagttcca
    18841 ggttgatagt tggattgcag ggatggaatc ttgtttttca aagccgcttc atatccctta
    18901 gtttgaacct tgatgtagtg attgtggtcg ccacgaggaa tcacaaaacc ttctgaatct
    18961 tcacttataa ttcgattggc atcaaaacca tgaccatctt cttcctcatg gtggacatgt
    19021 agtgacggat tacttaatac agaactagaa gaacttccta cctcttccgt gttagagtgt
    19081 gatgggggat tgttaagaga tgacttagga atatagtgat agtgacccca tgtcttacta
    19141 tataagcatc acctgtatct ctgacaatat cattagggtt aaagacataa ccatcatctg
    19201 ctgcagaaac accattattc ggtgtcaccg acaaagattg actgagagct gtagtattct
    19261 ctgataatta tacttttgca gctgctaatt cacctgccga caagtcactc tcaggaatga
    19321 aatgatagtg accaccatgt ggtactatag tagattgaaa tagaatatga gcaaattgat
    19381 aaggggattt taaagtaatt tctaacaatg atttagaaac tatgatgtgc tattctaaat
    19441 tcaactcact atatataacc atcatcggta gtataacgtc cctgtaattt tactacagat
    19501 acttctgcac tagctccttt atcgtcttta ccatgttctt gtttttggcg attgatttca
    19561 tcttttattc gtacattttc tgcatgagct tgatctttaa ggtaaacata atactttcca
    19621 tctaccttaa taatatatcc tcccttaacc taactgacga tatcttgatc tttcggctga
    19681 tagttggggg ctttcattaa tagctcttca ctaaagagcg catccaaagg aactttacca
    19741 ttatagcagt gataatgatc gccatgagaa gttacataac cttgatctgt aatcttaata
    19801 acaatttgtt ttgcttgaat tccttctttt tgactaacct agtctggagt caaattttca
    19861 gtcttcttag tgtctttatt actgtttaca tatgaaacac gatttttatc tgtattggcc
    19921 tgttagctat gttggttcag agcataaaca cacagactta aggaaaggat aacaacagat
    19981 ccagctgcta tatatttctt tttaaatttc ata
//
LOCUS       DAVLRT010000034        19643 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLRT010000034 DAVLRT010000000
VERSION     DAVLRT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.034"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            83..880
                     /locus_tag="VXM85_001185"
     CDS             83..880
                     /locus_tag="VXM85_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1345769.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..2287
                     /locus_tag="VXM85_001186"
                     /pseudo
     CDS             1028..2287
                     /locus_tag="VXM85_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2383..3657
                     /locus_tag="VXM85_001187"
     CDS             2383..3657
                     /locus_tag="VXM85_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1345770.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VXM85_001188"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VXM85_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1345771.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VXM85_001189"
     CDS             4786..5088
                     /locus_tag="VXM85_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1345772.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKHQS
                     VMADLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VXM85_001190"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VXM85_001190"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1345773.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VXM85_001191"
     CDS             5962..7302
                     /locus_tag="VXM85_001191"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1345774.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VXM85_001192"
     CDS             7280..7492
                     /locus_tag="VXM85_001192"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1345775.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VXM85_001193"
     CDS             7489..8364
                     /locus_tag="VXM85_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1345776.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQVPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VXM85_001194"
     CDS             8357..9172
                     /locus_tag="VXM85_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1345777.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VXM85_001195"
     CDS             9165..9596
                     /locus_tag="VXM85_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1345778.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VXM85_001196"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VXM85_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1345779.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDMTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VXM85_001197"
     CDS             11272..12000
                     /locus_tag="VXM85_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1345780.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VXM85_001198"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VXM85_001198"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1345781.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14106..14186)
                     /locus_tag="VXM85_001199"
                     /pseudo
     CDS             complement(14106..14186)
                     /locus_tag="VXM85_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            14359..14835
                     /locus_tag="VXM85_001200"
     CDS             14359..14835
                     /locus_tag="VXM85_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1345782.1"
                     /translation="MVLDYFFDKNLVLCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMNFFTGNGHLEDLSKISEPEKLYAYIDEAIA"
     gene            14873..15175
                     /locus_tag="VXM85_001201"
     CDS             14873..15175
                     /locus_tag="VXM85_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1345783.1"
                     /translation="MIKILAACGAGVNSSHQIKSTLEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSAPVFDNIRLPAKQNMV"
     gene            15183..15986
                     /locus_tag="VXM85_001202"
                     /pseudo
     CDS             15183..15986
                     /locus_tag="VXM85_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            16035..16499
                     /locus_tag="VXM85_001203"
     CDS             16035..16499
                     /locus_tag="VXM85_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1345784.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTSLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            16656..17081
                     /gene="lacA"
                     /locus_tag="VXM85_001204"
     CDS             16656..17081
                     /gene="lacA"
                     /locus_tag="VXM85_001204"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1345785.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            17112..17627
                     /gene="lacB"
                     /locus_tag="VXM85_001205"
     CDS             17112..17627
                     /gene="lacB"
                     /locus_tag="VXM85_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1345786.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            17638..18567
                     /locus_tag="VXM85_001206"
     CDS             17638..18567
                     /locus_tag="VXM85_001206"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1345787.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            18569..19549
                     /gene="lacD"
                     /locus_tag="VXM85_001207"
     CDS             18569..19549
                     /gene="lacD"
                     /locus_tag="VXM85_001207"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1345788.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQRTATSWKERV"
BASE COUNT         6003 a         3547 c         4501 g         5592 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg atacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat gatatggagg
     1021 tcaccttatg acttgtgatt ttaaatctga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatgttga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aaacttgtct tgattgaaac cttgggtaac tccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaata ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggatggctt cagggaaatt ccctcaattt gttgacgagg gttcaagctg
     1741 ccacaatttg agctatactc gtgatgtggg tgcagtagcc tttattatag ttgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa accctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttattat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtgacgagg aagaagcacg caaggtcatt gatcatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caatcactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaatc aaatccactt
     2221 gtctatcggt cttgaaaatg tagaagattt gattgaagac ttacgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg ttttgactca ctgtttttga ttttccttca
     2341 tgcatgatat aatggttaca gaagtctaga aagaggaacg atatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gagagtcagt atgccgaact cttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaagcagga actggccttg
     2521 gcggagcaaa aggccatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattac agagtcagat ccaaaacttt gatacagaaa aagaattggc caagaaagag
     2641 gttgaacaga caagccatga ggctctcttg gctaaggaca aggaagtaca gctcttagaa
     2701 aatcagttgg ctaccttgcg tttggagcat gaaaatcaac tacaaaagac cctttctgac
     2761 ctagaaaaag aacgggatca ggttaaaaac caactacttt tgcaggaaaa ggaaaatgaa
     2821 ttatctttgg cttctgttaa gcaaaactac gaagcccagc tcaaggcagc tagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcagac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat caacttaaaa aaagaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaattactc tgggttattc cacgacgact
     3901 gaggatgcta gtggggaagt gatcgcagaa acccctgttt tgtctccctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta cccagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgctcgatt cacttttcgt gtaaaatgca gtaaagggac ttacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctggt gggaacttag gcccaggatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaaca ccaatcggtc
     4921 atggccgatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggagcctcaa gatgtcttta
     5521 tcgttgaggg cattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgta ttattcgtcg tatcaagcgt gatatggagg
     5641 aacgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat aaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaaatat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggcctgttt caagaacgct tcaagcaagc tctaccccaa
     6361 ttttctaaga gaattggggt agtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatcctac caaggttcaa
     6481 ggtgaagggg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggacgat
     6541 ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattaactca tttgcaaaat
     6781 caggaaaaac ggatggcaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagat caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttgcca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttggagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagacccat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagattaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca cttgtctctg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gtaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ttgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattggaag aaattgccca gcaggttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgataccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 tttccgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta agattcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggca ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaaa
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattaccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgatacttt tgcagacaag atttttagtt gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgcctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct ctctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctga
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgctaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaatgaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagtatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattttt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacagtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatatgacag
    11221 aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tctcaaaacc
    11341 tgggatggtc agacccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtct
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcggcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatctttgt tcacgcaggt
    11701 attgatttga ccttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttaca
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gctttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacagga gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccac ggaaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggat
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatggag aaacttatat tttccacttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 gggattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aagtacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggttttc gttgtggatt tcttggactt
    13141 ctccatatgg atgttatcca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc aacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 atccttctca atggagataa ggtggatgct ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataagaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcatcgtcgc cttaccgtat
    14041 gtatggttac tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagctgact
    14101 tcgtcagtct tatctacaac ctcaaaactg tgttttgagt aacctgcggc tagcttccta
    14161 gtttgctctt tgattttcat tgagtattag aacagaaaaa tgcttggagt atttgtttgt
    14221 gtgtttattt ttatataaca aactataaac aaaacaaaaa tataaaaaag agacaaaaaa
    14281 gaacagaaag taattaacaa cgatttcata tagtgttata cttatggcat aaagttaatg
    14341 gaaagaaggg aaaaccttat ggtattagat tatttctttg acaaaaacct tgtgctttgc
    14401 ttagaagcgg ataatcaaga acaactcttt gatcaggtag caagcttgtt ggaagaacga
    14461 gaaattgtca ctcctactta tcgtgaagcc ttgatcacgc gtgaaaagtc atttccaact
    14521 ggtttagata tggaatttct aggaaaggac ttgccaaatg tagcgattcc tcacacagat
    14581 attgttcata atctagctga gaaagtggtg gttgttcgat tagaaaaacc agtaactttt
    14641 cacaatatga tagctcccga taaggaagta gaagtatccc tactcttctt tatcattaat
    14701 aattcaagtt caagtcaaac gaacattctt gctcagttga tgaacttctt cacaggaaat
    14761 ggacatcttg aagacctatc aaaaatttcc gaaccagaaa aactttatgc ttatatagat
    14821 gaagcaatcg cttaatcttg tctattaaaa aataaaatcg gaggaagtct atatgattaa
    14881 aattcttgct gcctgcggtg caggtgttaa ctcaagtcac caaattaaaa gtactctaga
    14941 agaagaactt tcaaaccgtg gttacgatgt tcactgtgat gcagtcatgg tgaaagatgt
    15001 aaacgaagac cttatgaaag gttacgatat ctttacacca atcgctgcaa cagatcttgg
    15061 ttttgaacca ggtatcccag ttatcgaagc tgggccaatc ttattttgta tcccagcaat
    15121 gagcgctcca gtatttgaca atattagact ccctgcgaaa caaaatatgg tatagtagtt
    15181 ctatgaatga tgaagcaagt aaacaactaa ccgatgcacg atttaagcgt cttgttggtg
    15241 ttcagcgtac cacttttgaa gagatgttag ctgtattaaa aacagcttat caacttaaac
    15301 acgcaaaagg tggacgaaaa cctaaattaa gcctagaaga ccttcttatg gccactcttc
    15361 aatatgtgcg agaatatcgc acttatgaac aattgcggct gattttggta tccacgaaag
    15421 caacttactc cgtcggagcc aatgggttga agtaactctt gttcaaagtg gttttacgat
    15481 ttcaagaact cctctcagtt ctgaggacac ggtaatgatt gatgcgacga aagtaaaaat
    15541 caatcgccct aaaaaaagaa ttagcgaatg attctggtaa aaagaaatgc cacgctatga
    15601 agtctcaggc gattgtcaca agtcaaggga gaattgtttc tttggatatc actgtgaact
    15661 attgtcatga tatgaagttg ttcaaaatga gtcgcagaaa tatcggacaa gctggaaaaa
    15721 tcttggctga cagtggttat caagggccca tgaagatata tcctcaagca caaactccac
    15781 gtaaatccag caaacttaag ccgctaatag ctgaagataa agcctataac tatgcgctat
    15841 ctaaggagag aagcaaggtt gagaacatct ttgccaaagt aaaaacgttt aaaatgtttt
    15901 caacaaccta tcgaaatcat cgtaaacgct tcggattacg aatgaatttg attgctggta
    15961 ttatcaatca tgaactagga ttctagtttt tcaggaagtc taataaagaa actagcaagg
    16021 tcaggcctta ctttattggt ggacatccag gatttaattg tcctctactt gatgatgaag
    16081 tctatgaaga ttattatcta gagtttgaga aagaagagac ttgctctgtt ccacgtcctt
    16141 tcccagaaac aggtatgttg gatttccaag atagaagtcc atggctagag ggacaaaaag
    16201 aaatagatct tagttatgat cttttcagca cagatgcagt gactttggat gaattgcaat
    16261 ctcgaacaat tgcccttcgt tctcttaaac atgataaggg attgaaagtg cattttgcag
    16321 aatttccaaa cctcatcatc tggtcaactt tgaacaaggg acctttcatt acctttgaac
    16381 catggtctgg cttgtcaaca tcccttgaag aaggagatca tttagaagat aagaagaatg
    16441 tttgtctctt agaagccaat caggttgaag aattagggtt tgagatagaa gttttataaa
    16501 aaagtaaaaa ataaataaca aaaacaaaca caaactattg acttcttcaa acaatagtag
    16561 tatattatgt ttgtgaggac taattgactg ttttttaaag atagccaata cactgctgga
    16621 atccagcata gaaaaaataa aaaggagtat acaatatgtc tattgttatc ggtgcagatg
    16681 ctgcaggttt gagattgaaa gaagttgtga aagacttctt agaaaaagaa aacttccacc
    16741 ttgtggatgt tacagctgaa ggtcaagact ttgttgatgt gactctcgct gttgctgcag
    16801 aagtaaacaa agaagaacaa aaccttggta tcgtgattga tgcttatgga gctggtccat
    16861 ttatggttgc aactaagatc aaaggaatgg ttgctgcaga agtatctgac gaacgttcag
    16921 cttatatgac tcgtggccac aacaactcac gtatgatcac tatgggggca caacttgttg
    16981 gtgatgaatt ggctaaaaac atcgctaaag gatttgttaa tggtaaatac gacggtggtc
    17041 gtcaccaaat ccgggttgac atgttgaaca aaatgggcta attagattat agtaagaaag
    17101 gtaagttaaa aatgagaatt gcaattggat gtgaccacat cgtaactgat gaaaaaatgg
    17161 cggtttcaga atttttgaaa tcaaaaggat atgaagtcat tgactttggt acatatgacc
    17221 atacacggac tcactaccca atctttggta aaaaagtagg ggaagcagta actagtggtc
    17281 aagctgatct tggggtatgt atctgtggta ctggtgttgg tatcaacaac gctgtaaata
    17341 aagttccagg tgttcgttct gccttggttc gtgatatgac aacagccctt tatgctaaag
    17401 aacaattgaa cgctaacgtt atcggctttg gtggtaagat tactggtgaa ttgcttatgt
    17461 gtgatatcat cgaagctttc attcatgctg aatacaaacc atctgaagaa aacaagaaat
    17521 tgattgcgaa aattgaacac cttgaaagtc acaatgctca acaaacagac gcaaacttct
    17581 ttacagaatt ccttgaaaaa tgggatcgcg gagaatacca cgactaagag gtgacctatg
    17641 attttaacag tcacaatgaa cccatccatt gatatttcct atcccttgga tgagttgaag
    17701 attgatactg tcaatcgtgt ggtggatgta accaaaacgg ctggtggtaa gggacttaat
    17761 gttacccgag tactttcaga atttggcgat tctgttcttg ctactggttt agtgggtggc
    17821 aaacttggtg agtttttggt tgaacatatc gataatcaag taaagaaaga tttcttctca
    17881 attcagggag aaactcgtaa ctgtatcgct attctccacg gagacaacca aacagaagtt
    17941 cttgaaaaag gtcctgaagt attggaacag gaaggtcaag actttttgga acatttcaaa
    18001 aaactcttgg agtcagttga agtagtggcc atctcaggta gtctgccagc tggccttcca
    18061 gttgattact atgcgagctt ggtagaactt gctaatcaag ctggcaagcc tgtagtcttg
    18121 gactgctcag gtgcagcact tcaggctgtt cttgaatcac cccataaacc aacagtcatc
    18181 aaaccaaata atgaagaatt gtctcagctt cttggaagag aagtttctga ggatttggat
    18241 gaattaaaag aagtacttca agaacctttg tttgcaggga ttgaatggat tatcgtttca
    18301 cttggtgcca acggtacttt tgccaaacat ggtgacactt tctacaaggt agatattcct
    18361 agaattcagg tggtaaatcc tgttggatct ggagactcta ctgtggcagg aatttcttca
    18421 ggacttcttc acaaagaatc ggatgcagaa ttactcatca aggcaaatgt ccttggtatg
    18481 ctcaatgctc aagaaaaaat gactggtcat gtcaacatgg ccaactatca agctctatat
    18541 gatcaattaa tagtaaaaga ggtataaaat ggctttaaca gaacaaaaac gtgtacgctt
    18601 agaaaaactt tctgatgaaa atggtatcat ctcagctctt gcatttgacc aacgtggtgc
    18661 tttgaaacgc ctcatggctc aacaccaaac agaagaacca actgtggccc aaatggaaga
    18721 acttaaagtc ttggtagcag atgaattgac taaatacgct tcatctatgc ttcttgaccc
    18781 tgagtatgga cttccagcaa ctaaagctct tgatgaaaaa gctggtcttc tccttgctta
    18841 tgaaaaaaca ggttatgaca caacaagcac aaaacgcttg ccagactgct tggatgtttg
    18901 gtctgcaaaa cgtattaaag aagaaggtgc agatgcagtt aaattcttgc tttactatga
    18961 tgtagatagc tcagacgaac tcaatcaaga aaaacaagcc tacatcgaac gcatcggttc
    19021 tgagtgtgtg gctgaagata tcccattctt ccttgaaatc cttgcttacg atgaaaaaat
    19081 tgcggatgca ggttctgtag aatacgctaa agtaaaacca cacaaagtta tcggcgctat
    19141 gaaagtcttt tcagacccac gctttaacat tgatgttttg aaagttgaag ttcctgttaa
    19201 cattaaatat gttgaaggct tcgctgaagg tgaagtagtt tatacacgtg aagaagcagc
    19261 agccttcttc aaagcgcaag atgaagcaac gaacttgcca tacatctact tgagtgctgg
    19321 tgtatcagct aaactcttcc aagatactct tgtatttgct catgaatcag gtgcaaactt
    19381 taacggagtt ctttgtggcc gtgctacatg ggcaggatca gttgaagctt acatcaaaga
    19441 tggtgaagca gcagctcgcg aatggcttcg cacaactgga tttgaaaaca ttgacgaact
    19501 caacaaagtt cttcaaagaa cagcaacttc atggaaagaa cgcgtgtaag aaagtcctcc
    19561 tagtttagga acatgaatct aaaaaaattt aaaaaaagtt gtatgtaaag gtttacaaaa
    19621 taacttactt gtgctatact taa
//
LOCUS       DAVLRT010000035        19061 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLRT010000035 DAVLRT010000000
VERSION     DAVLRT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.035"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            86..2308
                     /gene="recJ"
                     /locus_tag="VXM85_001208"
     CDS             86..2308
                     /gene="recJ"
                     /locus_tag="VXM85_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1345789.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFITFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2483..4144
                     /locus_tag="VXM85_001209"
     CDS             2483..4144
                     /locus_tag="VXM85_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1345790.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4213..4992
                     /locus_tag="VXM85_001210"
     CDS             4213..4992
                     /locus_tag="VXM85_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1345791.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5104..6324
                     /gene="murM"
                     /locus_tag="VXM85_001211"
     CDS             5104..6324
                     /gene="murM"
                     /locus_tag="VXM85_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1345792.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6329..7561
                     /gene="murN"
                     /locus_tag="VXM85_001212"
     CDS             6329..7561
                     /gene="murN"
                     /locus_tag="VXM85_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1345793.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQNKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7640..8341
                     /locus_tag="VXM85_001213"
     CDS             7640..8341
                     /locus_tag="VXM85_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1345794.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETNE"
     gene            8380..10224
                     /gene="uvrC"
                     /locus_tag="VXM85_001214"
     CDS             8380..10224
                     /gene="uvrC"
                     /locus_tag="VXM85_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1345795.1"
                     /translation="MNNLIKPKLELLPTSPGCYIYKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNEHYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKNDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVTAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            10214..11047
                     /locus_tag="VXM85_001215"
     CDS             10214..11047
                     /locus_tag="VXM85_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1345796.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLQGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEVLDGEYGLEYPKEIQSMRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSKGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQRHNLKLLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(11225..12025)
                     /locus_tag="VXM85_001216"
     CDS             complement(11225..12025)
                     /locus_tag="VXM85_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1345797.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKESSSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTYLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSN
                     YIVFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12196..12801
                     /locus_tag="VXM85_001217"
     CDS             12196..12801
                     /locus_tag="VXM85_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1345798.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVCEKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            12814..14214
                     /gene="pepV"
                     /locus_tag="VXM85_001218"
     CDS             12814..14214
                     /gene="pepV"
                     /locus_tag="VXM85_001218"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1345799.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFDFAGPAKEYLDITGKILLNDHEGESLKIAHVDEKMGALSMNAGV
                     FRFDETCADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14268..14846
                     /locus_tag="VXM85_001219"
     CDS             14268..14846
                     /locus_tag="VXM85_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1345800.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFVAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     DFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKYYLPAYFPLV
                     HPSPRNQIWMSKNPWFEAEVVPDLKKRIKTIL"
     gene            15006..15158
                     /locus_tag="VXM85_001220"
     CDS             15006..15158
                     /locus_tag="VXM85_001220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1345801.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDELAALHLEGTHNLPLSQ
                     LADSYD"
     gene            15201..15293
                     /locus_tag="VXM85_001221"
     CDS             15201..15293
                     /locus_tag="VXM85_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1345802.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15464..16789
                     /gene="brnQ"
                     /locus_tag="VXM85_001222"
     CDS             15464..16789
                     /gene="brnQ"
                     /locus_tag="VXM85_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1345803.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAVAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16887..17924
                     /locus_tag="VXM85_001223"
     CDS             16887..17924
                     /locus_tag="VXM85_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1345804.1"
                     /translation="MNQTINYIKELTAIASPTGFTREISDYLVHTLEKLGYQPVRTAK
                     GGVNVTIKGQNDEQQRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQTVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRVYKEEGIELPVTTHFAFSVFEE
                     VGHGANSNIPTQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            17926..18367
                     /locus_tag="VXM85_001224"
                     /pseudo
     CDS             17926..18367
                     /locus_tag="VXM85_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
     gene            18375..19061
                     /locus_tag="VXM85_001225"
                     /pseudo
     CDS             18375..19061
                     /locus_tag="VXM85_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5653 a         3529 c         4411 g         5468 t
ORIGIN      
        1 ttataaaaaa gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga
       61 attcttagaa agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct
      121 gcaggtagaa gatgcggatt ttacaaagat agccaagaag gctggactgg gtcctgaggt
      181 ggctcggtta ttgtttgaga gagggattca gaaccaagaa agtctgaaga agtttttaga
      241 accttccttg gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga
      301 gcggattcgt caggctattg aagaagggga aaatattctt gtttatggag actatgatgc
      361 ggatggcatg acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgctgagtg
      421 ccgagtttac ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa
      481 atactttatc gagcaagaag ggatttcctt gattgtgacg gtggacaatg gggttgctgg
      541 tcatgaggct attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca
      601 ttccatgcct gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc
      661 ggattatcct tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct
      721 gttagaagaa gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat
      781 ggtgagtctg acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca
      841 tacccagcgc attggtctgc aagaaatgct ggacatggct gggattgctg ccaacgaagt
      901 aacagaagaa acggttggtt tccagattgc tcctcgtttg aatgccttgg gtcgcttgga
      961 tgatcccaat cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat
     1021 tgcccttatg attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga
     1081 agaagccaag accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg
     1141 gaatcctggg gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt
     1201 cattgttctt aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt
     1261 cgatattttt gaagctctgg atccccatcg agacctcttc atcacctttg gaggtcatgc
     1321 aggtgcagcg ggtatgacgc tggaagttga gcaactctca gatttatctc aggttttgga
     1381 agattatgtt cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga
     1441 agagttggat ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc
     1501 ttttggaatg gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc
     1561 tcgtactatg ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggcgag
     1621 ttttgaagtg gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa
     1681 tctagagtta gcggttaaat tgtctgtcaa ccaatggaat ggccaaactg ccctccagtt
     1741 gatgatggtg gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc
     1801 agtcttgcca gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc
     1861 tagtgaagct gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt
     1921 tcaggaacag catttctctg ctgtctattt caaaaatgat attgacaaga cctattatct
     1981 gacaggttat gggactagag atcagtttgc taaattgtac aagactattt accagttccc
     2041 agagtttgat attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat
     2101 cttgctggtc aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg
     2161 tgtgatgaca gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca
     2221 aaatctcaaa caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat
     2281 ttatgatttt ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt
     2341 tttgaaaaca gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga
     2401 ttcggctgaa agtatcagaa cttttagaat aagagggtag aattgcccta taatcaagat
     2461 aaactaagat tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg
     2521 tgagaatgga aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt
     2581 agggttaaaa tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga
     2641 ttaccttttt gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga
     2701 tgccattggt gctctacctt atctcttggc agaggctaaa gttcctgtat ttgggtctga
     2761 gttgaccatt gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa
     2821 tgatttccat gtcattgatg agaatacgga gattgatttt ggtgggacag tggtttcctt
     2881 cttccctacg acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg
     2941 aagcatcgtt tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac
     3001 tgattttgct cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc
     3061 ggccaatgca gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac
     3121 ccaaactatt gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc
     3181 tcgtattcag cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg
     3241 atttgatatt gaaaatatcg tccgcacagc gattcgtctt aagaagttgt ctttagccaa
     3301 cgaaattctc ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat
     3361 tcttgagaca ggtcgtatgg gtgaacctat caatggactt cgtaagatgt cgattggtcg
     3421 ccatcgttat gtagaaatca aggatgggga cctggtctat attgctacgg ctccgtctat
     3481 tgctaaagaa gcctttgttg cgcgtgtgga aaatatgatt tatcaggcag gtggggttgt
     3541 caaattgatt acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct
     3601 gatgatcaat cttttgcaac ctaagtacct cttccctgtc caaggggagt atcgtgagtt
     3661 ggatgctcac gctaaggctg ccatggcagt tgggatgttg ccagaacgca ttttcattcc
     3721 taaaaagggg acgaccatgg cttacgagaa tggagacttt gttccagctg gatcggtttc
     3781 agcaggagat atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg
     3841 tgaccgtaag gtcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg
     3901 tgagaagaaa attgtggcta gggctcgtgt tcacacgcgt ggatttgttt atctcaagaa
     3961 gagtcgcgat attctccgtg aaagttcaga attgattaac caaacggtag aagattatct
     4021 tcaaggagat gactttgact gggcagatct taaagggaag gttcgagata atttgaccaa
     4081 gtatctcttt gaccaaacca agcgtcgtcc agctatttta ccagtagtca tggaagcaaa
     4141 ataatcgttg aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag
     4201 aaggagaaaa tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag
     4261 tggggggtga atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat
     4321 attcccgtct tgtacctttt gcacgggatg tctggaaatc ataatagttg gcttaagcgg
     4381 accaatgtag aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc
     4441 aatggttggt acaccgatac ccagtatggt tttgactact acacggctct agcagaggaa
     4501 ttgccgcagg ttctgaaacg cttcttccct aatatgacta gcaagcgtga aaagaccttt
     4561 atcgctggtc tttctatggg aggctacggc tgcttcaaac tggctcttac gacaaatcgt
     4621 ttttctcatg cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt
     4681 caaaatctgg gaagtccagc ctactggaga ggtgtttttg gagagattag agactggaca
     4741 actagtccct attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg
     4801 gcgtggtgtg gcgaacagga tttcttgtac gaagccaata atctcgcagt gaaaaatctc
     4861 aaaaaactag gttttgatgt gacctatagc catagtgctg gaactcacga gtggtactac
     4921 tgggaaaaac aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag
     4981 agactgactt agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc
     5041 actagacttt tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag
     5101 gaaatgtacc gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa
     5161 catgaattag ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa
     5221 catgagaagt ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt
     5281 agaactcttc cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat
     5341 ggggataaag aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag
     5401 agagcggttt ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag
     5461 gaaaagacag aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta
     5521 aggtggtcag gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa
     5581 atatacaagg aaaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga
     5641 acagcacgaa acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt
     5701 tcggagttga tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat
     5761 tataaaaaat tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat
     5821 gtttctaaac gttcgcaaga attagaagaa cagttagcga aaaatagagc cttggaagag
     5881 acctttactg agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt
     5941 ttgttagagg aattgacctt cttgcaggaa tatatagatg taggtcaagc gagagttcct
     6001 ttagcggcta ctttgagttt ggaatttggt actacctctg tcaatatata tgctggtatg
     6061 gatgatgatt ttaaacgtta caatgcacca attttaacat ggtatgaaac ggctcgctat
     6121 gcctttgagc gaggtatggt ctggcaaaat ttaggtggtg ttgaaaactc tctcaatggt
     6181 ggactttatc attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt
     6241 acaatgccca ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca
     6301 ttaagaaaaa aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt
     6361 tcagacttat tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga
     6421 tttgctagaa aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat
     6481 tcaagttgca gctctggttt atagcttgcc catgctgggt ggtctgcata tggaactcaa
     6541 ttcggggccg atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga
     6601 atatgccaag caaaatggtg tattagagtt gcttgtaaaa ccttatgaaa cttatcaaac
     6661 ttttgatagc caaggtaatc caatagatgc tgagaaaaaa agtattattc aaggtttgac
     6721 tgatttaggt tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg
     6781 gttatactat aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa
     6841 aaagggtaaa cccttggtga aaaaggctga aacctttggc attcgattga aaaagttaaa
     6901 acgtgaagaa ctatcgattt ttaagaatat aacaaaagaa acctctgaac gtagagaata
     6961 tagtgataaa agtttagaat attatgagca tttttatgat acttttggag aacaagcgga
     7021 gtttctcata gcaagcttaa atttttcgga ctatatgagc aaattgcaag gtgaacaaaa
     7081 taaactagaa gaaaacttgg acaagttgcg acttgatttg agtaaaaatc ctcattctga
     7141 gaaaaaacaa aatcaactga gagaatattc tagtcaattt gaaacgtttg aagttcgaaa
     7201 agcagaagcg cgagacttga ttgaaaaata tggagaagaa gatattgttt tagctgggag
     7261 tttatttgtt tatatgcctc aggaaacgac ttatctcttt agtggttcct acactgagtt
     7321 taataagttc tatgcccctg cactgcttca aaaatatgtt atgttggaaa gcataaaacg
     7381 tggaatacct aaatacaact tcctaggcat tcaagggatt tttgatggaa gtgatggtgt
     7441 tttgcgtttt aaacagaatt ttaatggcta tattgtacgc aaagcaggta ctttccgtta
     7501 ccatccatct cctttaaaat acaaagctat ccagttactc aaaaaaatag taggacgtta
     7561 agatgaaaaa gtcagtattt agatttcttt tagcttcttt tagtaaaata atttttattt
     7621 gctagaaagg tggagagaca tgcgctggct ttttcgtttg ataggggctt tcttttcttt
     7681 tgtgtggcgt ttgttttggc gtctggtttg gatagttgtg ctcttatgtg tgcttgcttt
     7741 cggacttctc tggtatctga acggggattt tcaaggagcg ctaaagcaag cagaacggtc
     7801 agtaaaaatt ggtcaacaaa gtattgacca atgggagaaa acagggcaac tgcctaagtt
     7861 aagccagaca gatagtcacc agcattctga aggaaggtgg ccacaggcct ctgctcgtat
     7921 ttacctggat ccgcagatgg attcacgctt tcaagaggct tatttagaag caatccagaa
     7981 ctggaatcaa actggtgctt ttaactttga actcgtgact gagtctagta aggcggatat
     8041 tacggctacg gagatgaacg acggaggcac tcctgtggca ggagaggcgg aaagtcaaac
     8101 taatctctta acagggcaat tcttgtccgt aacggtgcgg ttgaatcatt attatttgtc
     8161 caatccatac tatggctatt cctatgaacg ccttgtccat acggcagaac atgagttagg
     8221 tcatgcgatt ggcttggacc atacagatga gaagtctgtc atgcaaccag caggttcctt
     8281 ttatggtatc caggaagagg atgttgcaaa cctccgaaaa atatatgaga ctaatgagta
     8341 gggtactatc tttccctact tttttgctat aatggaacta tgaacaactt gattaaacca
     8401 aaactagagc tcttgccgac cagccctggt tgctacattt acaaggataa aaacggcacc
     8461 attatctatg taggaaaggc taaaaatctg cgtaatcgcg tgaggtctta tttccgtgga
     8521 agtcatgata ccaaaacaga ggccttggtg tctgaaattg tggattttga atttattgtt
     8581 acggagtcta atattgaggc gcttctgctg gaaatcaacc tgatcaagga aaataagccc
     8641 aagtacaata tcatgctcaa ggatgacaag tcttatcctt ttatcaaaat caccaatgag
     8701 cactatccac ggcttatcat tactcgtcag gtcaaaaagg acggtggtct ttattttggc
     8761 ccttatccag atgtgggggc agccaatgaa atcaagcgac ttctagatag gattttccct
     8821 tttcgaaaat gtaccaaccc gccctctaag gtctgttttt actaccatat cggccaatgt
     8881 atggcccaca ccatctgtaa gaatgacgag gcctatttca agtccatggc ccaggaggtg
     8941 tctgattttc tgaaaggtca ggatgacaaa atcatcgatg atctcaagag taaaatggca
     9001 gtaacagcac aaagtatgga gtttgaacgg gcagcggaat accgtgacct gattcaggcc
     9061 attggaacac ttcgaaccaa gcaacgggtc atggctaagg atctccaaaa tcgcgatgtc
     9121 tttggctact atgtggataa gggctggatg tgtgtgcagg ttttctttgt tcgtcagggc
     9181 aagctcatcg agcgcgatgt caatctcttc ccctacttca atgatccaga tgaggatttc
     9241 ttgacctatg tgggacaatt ctatcaagaa aaatctcacc tagttcccaa tgaggtactg
     9301 attccgcagg atattgacga agaagctgtc aaggctttgg tggataccaa gattctcaaa
     9361 ccccaacgtg gagagaaaaa acagctggtc aatctagcca taaaaaatgc tcgtgttagt
     9421 ctagagcaga agttcaatct gctagaaaaa tcagtcgaaa agacccaagg agctattgaa
     9481 aatctaggac gtttgctcca aattccgact ccagttcgca tcgagtcctt cgataactcc
     9541 aacatcatgg ggaccagtcc tgtttcagcc atggtggtct ttgttaacgg caggccaagt
     9601 aaaaaagatt accgtaagta caagataaaa accgttgttg ggccggacga ctatgctagt
     9661 atgagagaag tcattcgcag acgctatggt cgagtacagc gtgaggcttt gactcctcca
     9721 gatttgattg tgattgatgg ggggcaaggt caagtcaata ttgctaagca agtcatccaa
     9781 gaggaactgg gcttggatat tccaattgct gggctgcaaa agaatgataa gcaccaaacc
     9841 catgaattgc tctttggaga tccgcttgag gtggtggact tgtctcgcaa ttctcaggaa
     9901 tttttcctcc tccaacgcat ccaagatgag gtgcaccgct ttgctatcac tttccaccgc
     9961 caactgcgct ccaaaaattc tttctcatct caattggatg ggattgacgg tctgggacct
    10021 aaacgcaagc agaatcttat gaagcatttc aagtctttga ccaaaatcaa ggaagccagt
    10081 gtggatgaga ttgtcgaagt tggggtacct agagtcgttg cagaggctgt gcaaagaaag
    10141 ttgaacccgc agggagaagc cttgcctcaa gtagcagaag aaagagtaga ttaccaaacg
    10201 gaaggaaacc acaatgaacc ataaaatcgc aattttatca gatgttcatg gcaatgcgac
    10261 ggcgctagaa gcagtgattg cagatgctaa aaatcaaggg gccagtgaat attggcttca
    10321 gggagatatt tttcttcctg gtccaggcgc aaatgactta gtcgccctgc taaaggacct
    10381 tcctatcaca gcaagtgttc gaggcaattg ggatgatcgt gtccttgagg tcttggatgg
    10441 cgaatatggt ttggaatatc cgaaagaaat ccagtcaatg cgcatgaccc agtttttgat
    10501 ggagcgaatg gatcctgcaa cgattgtctg gctacgaagc ttgcctttgc tggaaaagaa
    10561 agaaattgac ggattgcgct tttctatctc tcataattta cctgacaaaa actatggtgg
    10621 tgacttgcta gttgagaatg atacagagaa atttgaccaa ctgctagatg cggaaacgga
    10681 cgtggcagtt tatggtcatg ttcacaagca gttgcttcgc tacggcagta aagggcaaca
    10741 aatcatcaat ccagggtcga ttggcatgcc ctattttaat tgggaggcgt taaaaaatca
    10801 ccgtgcccag tatgccgtga tagaggttga agatggggaa ttactcaata tccaatttcg
    10861 taaagttgct tatgattacg aggctgagtt agaattggcc aaatccaagg ggcttccctt
    10921 tatcgaaatg tatgaagaac tacgtcgtga agataactat cagaggcaca atctgaaact
    10981 attagcaagc ttaatagaaa agcataggta tgtagaggat gtgaagaatt tttttgattt
    11041 tttgtaagag tttcctaaaa taaccaatgc aaactaaaaa cgattggtag tccaatcgct
    11101 tttatatatc ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgtaggttg
    11161 ctcaaagcac agctttgagg ttgcagataa agctgacgtg gtttgaagag attttcgaag
    11221 agtgttattg taactgagat tgatctggga ggtaagaacc acctagatag gtattgctga
    11281 gtttttcaag ggttccgtct tgatagagtt ctttgagcgc tttatcaaat ttctctttaa
    11341 actctttttg gtcacttgag aaaacgatat agttgctggg gctatctgta gaaggtaaat
    11401 caacgactga gaggtctaaa ccacggtcct tgataatctt ttgaacggat accttgtcaa
    11461 aaactaggaa atcaaactct ccgttagcaa ggtctaggat tcgtttacca atatcctcac
    11521 cagaaaaatc aattgtagcg ggattatcag tgtgtttctg attccagtta ttgatgaatt
    11581 gagcgttaga agttccggta tcctcttgtg ttgttttacc agcgatctgg tcaagataag
    11641 tcaaaggatt tttcttgttg ctgacaagga cgaggggatt gttggaaatt ggaagcgagt
    11701 agaggtattt ttcagcgcgc tcttttgtgt aactcaaatt attagctgct gcttgatagt
    11761 gaccagaatc aagtcctggg aagatgctct cccaagcagt tctttggaat tgaatctcgt
    11821 aatcgctgag tttttcatcc actgctttta ggacttcgat atcgaagcct gtcagattgc
    11881 ccttgtcttc gtagtcaaat ggtggcacat cgccagctgt agcaaggacg attgtctttt
    11941 gagagctgga ctctttgggt gtagcttgat tcccacaggc aaccaaaaat ggtaggatag
    12001 ctagtaatag gctaaatttt ttcatactgt ctccattcaa atgtaaagta cttgctagtt
    12061 tatcagaaac tttcctgata aaccaatata tatcttttat gtctgtgata gaaaaactta
    12121 atcatgaaaa atccctgcaa gctctttcaa attatggtag aatggaagca gtagaattag
    12181 aaaaggaaat cgattatgaa atttcttgaa ttaaataaaa aacgtcatgc gaccaagcat
    12241 ttcactgata agcctgttga tccaaaagat gtgcgtacgg ctatcgaaat tgcaaccttg
    12301 gcgccaagcg cccacaacag ccagccttgg aaatttgtgg tggtatgtga gaaaaatgct
    12361 gaactggcaa agttagctta tggttccaat tttgaacagg tatcatcagc acctgtaacc
    12421 attgccctat ttacagatac agaccttgcc aaacgcgctc gtaagattgc ccgagttggt
    12481 ggtgctaaca atttctcaga agaacaactt caatacttca tgaaaaattt gccagctgaa
    12541 tttgcacgct acaatgaaca acaagtcagc gactacctag cactcaatgc aggtttggtt
    12601 gctatgaact tggttctcgc tcttacagac caaggaattg gttccaatat tatccttggt
    12661 tttgacaaat caaaagttaa tgaagttttg gaaatcgaag accgtttccg cccagaactc
    12721 ttgatcacag tgggttacac agacgacaaa ttggaaccaa gctaccgctt gccagtagat
    12781 gaaatcatcg aaaaaagata ggaagaagaa aaaatgatag caattgattt tacagcagaa
    12841 gtagaaaaac gcaaagaaga cctcttggct gacttgttta gccttttgga aatcaactca
    12901 gaacgtgatg atagcaaggc tgatgcccaa catccatttg gacctggtcc agtaaaagcc
    12961 ttggaaaaat tccttgaaat cgcagaccgt gatggttatc caactaaaaa tgttgataac
    13021 tatgcaggac attttgagtt tggtgatgga gaagaagttc tcggaatctt tgcccatatg
    13081 gacgtggttc cagctggtag cggttgggat acagatcctt acacaccaac tatcaaagat
    13141 ggtcgccttt atgcgcgcgg ggcttcggac gataagggtc ctacaacagc ttgttactat
    13201 ggtttgaaaa tcatcaaaga attgggtctt ccaacttcta agaaagttcg cttcatcgtt
    13261 ggaacagacg aagaatcagg ctgggcagat atggactact actttgagca cgtaggactt
    13321 gccaaaccag atttcggttt ctctccagac gctgaattcc ctatcatcaa tggtgaaaaa
    13381 ggaaatatca cggaatacct ccactttgca ggagaaaata caggtgctgc ccgtcttcac
    13441 agctttacag gtggtttacg tgaaaatatg gtaccagaat cagcaacagc agtcgtttca
    13501 ggtgatttgg ctgacttgca agctaaacta gatgcctttg ttgcagaaca caaacttaga
    13561 ggagaactcc aagaagaagc tggcaaatac aaggtgacga tcattggtaa atcagcccac
    13621 ggtgctatgc ctgcttcagg tgtcaatggt gcgacttacc tagccctctt ccttagccag
    13681 tttgactttg ctggtccagc caaagaatac cttgacatca ctggtaaaat tctcttgaac
    13741 gaccatgagg gtgaaagtct caagattgct catgtggatg aaaagatggg tgccctttct
    13801 atgaatgcag gcgtcttccg cttcgatgaa acatgtgctg ataataccat tgccctcaac
    13861 atccgctatc caaaaggaac aagtccagaa caaatcaagt caatcctaga aaacttgcca
    13921 gttgcttctg tcagtctatc agaacatggt catacccctc actatgtgcc aatggaagat
    13981 ccacttgtgc aaaccttgtt gaatgtctat gaaaaacaaa caggcctaca aggtcatgaa
    14041 caagtcatcg gtggtggaac ctttggtcgc ttgttagagc gcggagttgc ctatggtgct
    14101 atgttcccag actcaattga tactatgcac caagccaatg aatttattgc cttggacgat
    14161 ctcttccgag cagcagcaat ttatgccgaa gctatttacg aattgatcaa ataaaacgat
    14221 agaagtctga gatcttatgc ttggacttct ttttggaggg aaagtagatg tctcaaatcg
    14281 aaagaatcaa acaggctatc atggcggatt cgcagaatgc cagctataca gagcgtggca
    14341 ttgagcctct ctttgtagcg ccaaaaactg ctcgcatcaa tatcatcggt caggctccgg
    14401 gacttaaaac tcaagaagca ggcctttact ggaaagataa aagtggtgac cgcttgcggg
    14461 actggctagg tgtggatgaa gatacctttt acaattcagg ttattttgct gttttgccta
    14521 tggatttcta ctttccagga catggcaagt cgggtgatct tccgcctcgt acagattttg
    14581 cagaaaaatg gcatccgcag ctcttacagg aattgcctga tattcagtta accctcttga
    14641 ttgggcaata tgcccaagcc tactatttac aggagaaaat cagtgggaag gtaacggaga
    14701 gggtgaaaca ctataaatac tatctgccag cctattttcc gctagttcac ccatcaccac
    14761 gaaatcaaat ctggatgtcc aaaaatcctt ggtttgaggc agaagtagtg ccagatttga
    14821 aaaaaagaat taaaaccatt ttatagtcaa tgaaaatcaa agagcaaact aggaagctag
    14881 tcgtaggctg ctcaaagtac agctttgaag ttgcagataa aactgacgaa gtcggtaaca
    14941 tacgcacggt aaggcgacgc tgacgtggtt tgaagagatt ttcgaagagt attagaagaa
    15001 aaagaatgaa agaaatagcc tttgacgcat tttatcagct ttaccaaaac gaccagcttt
    15061 ctctagtgga tgtgagagaa gtggatgagc ttgcagctct tcatttagaa ggtacccaca
    15121 acctaccgct tagtcaattg gctgatagtt atgattaatt ggacaaagat cgcttgcatt
    15181 atattatttg caaatctgga atgagatcgg cgcgtgcttg ccaattcctg ttagaacaag
    15241 gttataatgt tatcaatgtc cagggtggca tgttagcctt tgaagaactt taaaattttg
    15301 catttctcct acttggtgtg gactgggtag gagagttttg tttttagata attcttattt
    15361 ttaagaaaat tgaaaacatt taatatttgc ctcgtgatgc ttttttcaga ctcctaatcg
    15421 tggtatacta ggtcagtatt ttataaatat gaaggagatt tttatggcta aaaaaggtac
    15481 cctaacaggt ttgctcctgt ttggaatatt ttttggtgcg gggaacttga tttttccgcc
    15541 ttctctaggt gctctatctg gagaacattt tcttcctgcc atcgcaggtt ttgtcttttc
    15601 aggcgttggt atcgccgtct tgacccttat tattggaacg ctaaatccta aaggatatat
    15661 ctacgagatt tcaacgaaga tagcgccttg gtttgcgact ctttacctct cagttcttta
    15721 cttgtcaatc ggtccattct ttgctacccc acgtactgct acaacagctt acgaagtagg
    15781 gattagcccc cttttgtcgg atgcaaataa aggacttggc ttgattgtat ttacggttct
    15841 gtattttgcg gtagcctatt tgatttcgct taatccatca aaaatcttag accgcattgg
    15901 acgtatttta acgccagtct ttgcaatttt gattgttatc ttggtcgttc tgggagctat
    15961 caaatatggt ggaacaagtc ctcaagctgc ttcagctgct tatcaagctt ctgcctttgg
    16021 tacaggtttc ctagaaggtt ataatacctt ggacgccctt gcctcagtgg cctttagcgt
    16081 aatcgcagtt caaaccttga aacaacttgg attttcaagt aagaaagaat acatttcaac
    16141 tatttgggtt gttggtatcg ttgttgccct tgccttcagc gctctttaca tcggtttagg
    16201 ttttcttgga aatcatttcc cagtaccagc tgaagcgatg aagggtggaa caccaggtgt
    16261 ttacatcttg tcacaagcca ctcaagaaat ctttggctca acagctcaac tcttccttgc
    16321 agctatggtt accgtaacct gcttcacaac gactgttggt ttgattgtgt caacagctga
    16381 gttctttaat gagcgcttcc cacaaatcag ctacaaggtt tatgcgacag cctttacctt
    16441 gattggattt gctattgcca atttgggtct tgatgcgatt atcaagtact caattccagt
    16501 actggttatc ttgtacccaa tcacgattgc tatcgttatg attgtcattg tcaacaaatt
    16561 tgttgccctt tcaaaaccag gtatgcagtt gacaattgct gtggttacag ttattgccat
    16621 tgcaagcgta ctaggaagct cgtttaaggt tgagtttctt gcaaatcttg ttagcgttct
    16681 tccttttgcc aaggcatctc tcccatggtt ggtgccagcc attgttggaa tcttgctctc
    16741 attggttcta ccaaacaagc aagaaagcga tgtttttgaa atggaataat cacttaaatc
    16801 acttttgtag ccaagtctac aggagtgatt ttcttttttt atccgatgat aaatgtgtta
    16861 taataggtag cgaaagaggt gaagaaatga atcagactat aaactatatc aaagaactaa
    16921 ccgctattgc atctccaacg ggctttactc gtgagatctc agactatcta gtccatactt
    16981 tagagaaact tggttaccag cctgttcgta cagctaaggg tggtgtcaat gtgaccatta
    17041 aaggtcaaaa tgatgaacaa caacgctatg tgacagccca tgtggatacg ctgggtgcta
    17101 ttgttcgtgc agtcaaacca gatggccgtc tcaaattgga tcgtattggt ggttttcctt
    17161 ggaacatgat tgaaggagaa aactgtaccg ttcatgtggc tagcacaggt caaacggtat
    17221 ctgggaccat cctcatccac cagacttctt gtcatgtcta caaggatgca ggaactgcag
    17281 aacgtacgca ggacaatatg gaagtgcgtt tggacgcaaa agtaaccaat gaaaaagaaa
    17341 cgcgcgcctt gggtattgag gttggagact ttattagttt cgatccgcga actgttgtga
    17401 cagagactgg ttttatcaag tctcgccact tggatgacaa ggtcagcgca gcaattttgc
    17461 tcaatcttct tcgtgtttat aaggaagagg gaattgaatt gccagtaaca actcattttg
    17521 ctttttcagt tttcgaagag gtgggccacg gtgctaattc caatattcca actcaagtgg
    17581 tggaatatct agctgtcgat atgggggcaa tgggagatga ccagcagaca gatgagtaca
    17641 cagtatctat ctgtgtcaag gatgcttcag gtccttatca ctatgacttc cgtcaacact
    17701 tggttgcctt ggctaaagag caagatattc cttttaagct ggacatctat ccattttatg
    17761 gttcggatgc ttcagcggct atgtctgcag gggcagaggt caagcatgcc cttcttggtg
    17821 cgggtatcga gtctagtcat tcttatgagc gcactcatat tgactcggtg gtcgcaacag
    17881 aacgaatggt cgatgcctat cttagaagca atttagtaga ttgatatgtg ccttatttgc
    17941 cagagaattg agctcatcaa gaagggagaa aatccctact ttgtcaaaga gttgggaaca
    18001 ggctatcttg tgattggaga ccatcagtat tttgcaagct atagtctctt tctagccaag
    18061 gaacatgtca cggaattgca ccattttaga aaaggacaca agactccatt ttctagaaga
    18121 aatgagttta gtccaagagg cagttgccaa gggctttact gctgagaaaa tgaatattga
    18181 acttctagga aatggcgatg cccatctcca ctggcacctt ttccctagac gaagaggtga
    18241 tatgaatggc cacggtctca aggactgtgg gccagtctgg tgggtccctt ttgaaaaaat
    18301 gaccgcagaa acccgccaag caaaaccgga tgagattaga cttcctgcga aacaaaatat
    18361 ggtatagtag ttctatgaat gatgaagcaa gtaaacaact aactgatgca cgatttaagc
    18421 gtcttgttgg tgttcagcgc acgacttttg aagagatgct agctgtatta aaaacagctt
    18481 atcaacttaa acacgcaaaa ggtggacgaa aacctaaatt aagcctagaa gaccttctta
    18541 tggccactct tcaatatgtg cgagaatacc gcacttatga agaaattgcg gctgattttg
    18601 gtgttcacga aagcaactta ctccgtcgga gccgatgggt tgaagtaact cttgttcaaa
    18661 gtggtgttac gatttcaaga actcctctca gttctgagga cacggtaatg attgatgcga
    18721 cggaagtaca aatcaatcgc cctaaaaaaa gaattagcga atcattctgg taaaaagaaa
    18781 tttcacgcta tgaaggctca agcgattgtc acaagtcaag ggagaattgt ttctttggat
    18841 atcgctgtga actatagtca tgatatgaag ttgttcaaaa tgagttgcag aaatatcgga
    18901 caagctggta aaatcttggc cgacagtggt tatcaagggc tcatgaagat atatcctcaa
    18961 gcacaaactc cacgtaaatc cagcaaactc aagccgctaa cagctgaaga taaagcctgt
    19021 aaccatgcgc tatctaagga gagaagcaag gttgagaaca t
//
LOCUS       DAVLRT010000036        18175 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLRT010000036 DAVLRT010000000
VERSION     DAVLRT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18175
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.036"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            291..1337
                     /gene="pheS"
                     /locus_tag="VXM85_001226"
     CDS             291..1337
                     /gene="pheS"
                     /locus_tag="VXM85_001226"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1345805.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1337..1846
                     /locus_tag="VXM85_001227"
     CDS             1337..1846
                     /locus_tag="VXM85_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1345806.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1923..4328
                     /locus_tag="VXM85_001228"
     CDS             1923..4328
                     /locus_tag="VXM85_001228"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1345807.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQINVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARDGEKLVTLDGEERDLAETDLVITVADKPVALAGVMGGQATEISEKS
                     SRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVIQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIVSLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4396..5397)
                     /locus_tag="VXM85_001229"
     CDS             complement(4396..5397)
                     /locus_tag="VXM85_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1345808.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5553..5968
                     /locus_tag="VXM85_001230"
                     /pseudo
     CDS             5553..5968
                     /locus_tag="VXM85_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6094..6360
                     /locus_tag="VXM85_001231"
     CDS             6094..6360
                     /locus_tag="VXM85_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1345809.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            6621..8870
                     /gene="metE"
                     /locus_tag="VXM85_001232"
     CDS             6621..8870
                     /gene="metE"
                     /locus_tag="VXM85_001232"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1345810.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEANRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8934..9800
                     /gene="metF"
                     /locus_tag="VXM85_001233"
     CDS             8934..9800
                     /gene="metF"
                     /locus_tag="VXM85_001233"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1345811.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10359..12572
                     /gene="pnp"
                     /locus_tag="VXM85_001234"
     CDS             10359..12572
                     /gene="pnp"
                     /locus_tag="VXM85_001234"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1345812.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDYKPKKEFTETPKDSE"
     gene            12588..13205
                     /gene="cysE"
                     /locus_tag="VXM85_001235"
     CDS             12588..13205
                     /gene="cysE"
                     /locus_tag="VXM85_001235"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1345813.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13217..14101
                     /locus_tag="VXM85_001236"
     CDS             13217..14101
                     /locus_tag="VXM85_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1345814.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14183..15526
                     /locus_tag="VXM85_001237"
     CDS             14183..15526
                     /locus_tag="VXM85_001237"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1345815.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLAAQGIKLLDTKDGVRWTRD"
     gene            15519..15905
                     /locus_tag="VXM85_001238"
     CDS             15519..15905
                     /locus_tag="VXM85_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1345816.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            15909..16793
                     /locus_tag="VXM85_001239"
     CDS             15909..16793
                     /locus_tag="VXM85_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1345817.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            16930..17385
                     /locus_tag="VXM85_001240"
     CDS             16930..17385
                     /locus_tag="VXM85_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345818.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            17382..17528
                     /locus_tag="VXM85_001241"
     CDS             17382..17528
                     /locus_tag="VXM85_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345819.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            17671..17841
                     /locus_tag="VXM85_001242"
                     /pseudo
     CDS             17671..17841
                     /locus_tag="VXM85_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         5480 a         3496 c         4123 g         5076 t
ORIGIN      
        1 gctaacaagc agaagtaaga taggtcttgc aagaatggta aaaatctgat aaactaagta
       61 gtaattgaat aggaatctga aagactagta gcccaaggga agtatatcag ggaggagagc
      121 cgtgactgca agctctctat atgaaagctg ggtgaattca cttgcgcatg gatttagaaa
      181 tgaaggtctg acttgtcaga tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag
      241 tttttggcat gtggttttct ttttatctac gagagactga tggaggaaat atgtcaacta
      301 ttgaagaaca attaaaagcg cttcgcgaag aaacgctggc tagcttgaag cagattactg
      361 ctggaaatga aaaagagatg caagatttgc gtgtctctgt ccttggtaaa aagggttcgc
      421 tcactgaaat cctcaaaggg atgaaagatg tttctgctga gatgcgtcca atcatcggga
      481 aacacgtcaa tgaagctcgt gatgtcttga ctgctgcttt tgaagaaaca gctaagctct
      541 tggaagaaaa gaaagtcgcg gctcaactgg ctagcgagag tatagatgtg acgcttccag
      601 gtcgtccagt tgcgactggt caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag
      661 atatcttcat cggtatgggt tatcaagtcg tggatggttt tgaagtggag caagactact
      721 ataactttga acgtatgaac cttccaaaag accacccagc tcgtgatatg caggatactt
      781 tctatatcac tgaagaaatc ttgctccgta cccacacgtc tccagttcag gcgcgtgcta
      841 tggatgccca tgatttttct aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc
      901 gtcgcgatac ggacgatgcg acccacagtc accaattcca ccaaatcgaa ggcttggtag
      961 ttgggaaaaa tatctctatg gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa
     1021 tgtttggtga agagcgtcaa attcgtttgc gtccatctta cttcccattc acagagccat
     1081 ctgttgaggt ggatgtttct tgcttcaagt gtggtggaga aggctgtaat gtatgtaaga
     1141 aaacaggttg gatcgaaatt atgggggccg gtatggttca cccacgtgtc cttgaaatga
     1201 gtggtatcga tgcgactgta tactctggct ttgcctttgg tcttggacaa gagcgtgtag
     1261 ctatgctccg ttatggaatc aacgatatcc gtggattcta ccaaggagat gtccgcttct
     1321 cagaacagtt taaataatga ttagaaaagt agaaatggca gatgttgagg tgttggctaa
     1381 aattgccaaa caaacctttc gtgaaacctt tgcgtatgat aatacggaag agcagttaca
     1441 ggaatacttt gaagaggctt atagtctgaa aactttgtca actgagttgg gaaatcctga
     1501 ctctgaaacc tatttcatta tgcatgagga ggagatagct ggttttctca aagtcaactg
     1561 gggaagtgct caaactgaga gagaattaga ggacgctttt gaaattcaac gcctctatgt
     1621 gctacaaaaa ttccaaggat ttggactagg taagcaactg tttgaattcg cacttgaact
     1681 tgctacaaaa aatagttttt cttgggcttg gctaggtgtt tgggagcata atacaaaagc
     1741 tcaagcgttt tataaccgat atggttttga aaaatttagc caacatcatt ttatggttgg
     1801 tcaaaaagta gatacggatt ggttactgag aaagaaatta aggtaagaag aggattcttc
     1861 tattgaaatg ataggaaagg aaaagaacta gagtgacaac tgtcattctg gagaactaaa
     1921 ttatgcttgt atcttataaa tggttaaaag aattggtgga cattgatgtg ccatcacaag
     1981 agttggctga aaaaatgtca actacaggga tcgaggtaga gggtgtcgaa tcaccagctg
     2041 ctggtctctc aaaaattgtc gtcggtgagg tcttgtcttg cgaagatgtg ccagagactc
     2101 acctccatgt ttgtcagatt aacgttggcg aagaagaaga gcgtcagatc gtttgtggtg
     2161 ccccaaatgt gcgtgctggg atcaaggtca tggtggctct tccaggagct cgtatcgctg
     2221 ataactacaa aatcaaaaaa ggaaaaatcc gtggtttgga gtcacttgga atgatctgtt
     2281 cacttggtga attgggaatt tctgactcag ttgtgcctaa ggaattcgca gatggcatcc
     2341 aaatcttgcc tgaagatgcc gtgccaggtg aggaagtctt ttcttaccta gacttggatg
     2401 atgaaatcat cgaactttcc atcacaccaa accgtgcaga tgccctttct atgtgtggag
     2461 tggctcacga agtggcagcc atctatgaca aggcagtcaa ctttaaaaaa tttactctaa
     2521 cagaaactaa tgaagctgcg gcagatgccc tttctgtcag cattgagaca gacaaggcgc
     2581 cttactatgc agctcgtatc ttggacaatg tgactatcgc accaagtcca caatggttgc
     2641 aaaaccttct catgaacgaa ggcatccgtc ccatcaataa cgttgtagac gtgacaaact
     2701 acatcctgct ctactttggt caacctatgc atgcttttga cttggacaca tttgaaggga
     2761 ctgacatccg tgtgcgtgaa gcgcgtgatg gtgaaaaatt agtgaccctg gacggtgaag
     2821 aacgagactt ggctgagaca gacctcgtga ttacagttgc tgacaaacca gtagcccttg
     2881 ccggtgttat gggtggtcag gctacagaaa tttctgaaaa atctagtcgt gttatccttg
     2941 aagctgctgt ttttaatggc aaatctatcc gtaagacaag tggtcgcctg aaccttcgtt
     3001 ctgagtcatc ttctcgcttt gaaaaaggaa ttaatgtggc aacagttaat gaagcccttg
     3061 atgcggcagc tagcatgatt gcagagcttg caggcgcgac ggtgcgtaag ggtatcgttt
     3121 cagcgggtga gcttgatacc tctgatgtgg aagtttcttc aacccttgct gatgttaacc
     3181 gtgtcctcgg aactgagctg tcttatgctg atgtagaaga cgtcttccgt cgtcttggct
     3241 ttggtctttc tggaaatgca gacagcttta cagtcagcgt accacgtcgt cgttgggata
     3301 tcacaatcga agctgatctc tttgaagaaa tcgctcgtat ctatggatat gaccgcttgc
     3361 caaccagcct tccaaaagac gatggtacag ctggtgaatt gactgtgata caaaaactcc
     3421 gccgtcaagt tcgtaccatt gctgaaggag caggtttgac agaaatcatc acctatgctc
     3481 tgacaactcc tgaaaaagca gttgagttca cagctcaacc aagtaacctt actgaactca
     3541 tgtggccaat gactgtggat cgttcagtcc tccgtcaaaa tatgatttca gggatccttg
     3601 ataccgttgc ctacaacgtg gctcgtaaga ataaaaactt ggccctttat gagattggaa
     3661 aagtctttga acaaacaggt aatccaaaag aagaacttcc aaatgagatc aacagctttg
     3721 cctttgcctt gacaggcttg gttgctgaaa aagatttcca aacagcagca gttccagttg
     3781 atttcttcta tgctaaggga atccttgaag ccctatttac tcgtttggga ctccaagtaa
     3841 cctatacagc aacatctgaa atcgttagcc ttcatccagg tcgtacagcc gtgatttcac
     3901 tcggtgacca agttcttggt ttccttggcc aagtgcatcc agtcactgcc aaggcttacg
     3961 atattccaga aacgtatgta gctgagctta acctttcagc catcgaaggg gcgctccaac
     4021 ctgctgttcc atttgtggaa atcaccaaat tcccagcagt cagccgtgac gttgcccttc
     4081 tcctcaaggc agaagtgact caccaagaag ttgtagatgc tatccaagct gccggcgtga
     4141 aacgtttgac agatatcaaa ctctttgacg tcttctcagg tgaaaaactg ggacttggta
     4201 tgaagtcaat ggcttatagc ttgaccttcc aaaatccaga agacagctta acggacgaag
     4261 aagtcgcacg ctatatggaa aaaatccaag cgtctctcga agaaaaagtg aatgcagaag
     4321 tgcgttaaac gatagtagga tacaaattcc ccctctagtt tatgaacatt agaggggtga
     4381 ttttatataa aataattaaa atgtgatttc tgtaaaaata catctatgat cagagttttg
     4441 aaaacttaca atgtctaaat ctttaacata gtagtggttt ttaggcaata aaatatgatc
     4501 tattgttgca ttaaaaagtt ttggactttg gctattccaa gttcctcttt caaaaggtgg
     4561 cagtgcattt aatgcgtccc tatgagagct tatttttgca agtgctccat gacgcatagt
     4621 tgcattaaaa tcacctgcaa taatagcctt tggatatttt gaagccaatt gattatgaat
     4681 gatgtttaag tcttgcttcc agatttccat taaacctggc agaggaggag cagtatgcaa
     4741 ggcaatgata tctggtatat tttgttttct cgaatgtaat acaattgtcc cgaaccgtgt
     4801 tgtatgaaaa gttttagctt ctgtatagaa accataactt ttcttgacaa tcacagtcac
     4861 aggagctatt ccactattgg taggtggaga agtgaaaata tcatagttgg ccatagaaag
     4921 tccaacttga tgaaacaata gtttgattct ctggttttct tgctcacctc tgatattggt
     4981 agctagttca gggaatatag ccatatcggc gtcaaaatgg ctaaaaattc gctcaatatg
     5041 ttgtgcttct atttgattag cgacgttcca agttactaat tttaaggttt tagtcttatt
     5101 tgatgcagaa gaaagggttt tatctgttcc gaataatagt aatatgagcg atagtaagag
     5161 caaaatgcca gagtaaaacc gtatttgttt atttttcttg taaaaagata ggaaaaaaaa
     5221 taagaaactt aaaatagcag ccatagttgc agtagctaag gggaaggcat aaatctgtgc
     5281 caagataggt tgggtagata gccaaatgtt aagattagaa gatgggaaaa aagaataagt
     5341 tatgataggg atagaaaaga gaatgaccga aagggcagag agaaatgttt taatcattgt
     5401 gtgagtacct caatttgcaa tggttgctcg gagctaatgt gactttgtga ctttacttga
     5461 ttgatattct ttttcttgtc ttcctcatag attaagtaca acccaagtaa aaaaagtcca
     5521 atttaagatt gaaatgatta aaaggcacag aaatgcaaga caattatact acaaaaggca
     5581 aacatttgac aatcgatagc cgtcgcttaa tcgaacgatg gaaaaaagaa ggaaaatcaa
     5641 atagagaaat tgcctctcta cttggaaaag ctcctcaaac tatccacact gaaatcaagc
     5701 gtgggacagt ccgacaatgt cttggaaaag ggcgcttcaa agagatttat tctgccgact
     5761 acgctcaaca gtcttaagaa actaaaggaa aagattctcc actatacata accaaaaaca
     5821 taaccaaaaa ttttcgcctg aaatgatggt gatggctaaa ggggttaacg tgggaatttc
     5881 aaccatttac tattggattc atcgtggaaa attggggtta agcaagcagg aactgcttta
     5941 tcctagaaaa ggaaaatcgc ttaagaaata ggctagcacc aactttaaac ccactggtca
     6001 atctatcgaa tcataattcg ctcatttttt taaaaataca tagacgtaaa aaaaagaggg
     6061 ttaagtgatg aagaatagtg ctattgggag taattggaag gatgtccatc tgagctcttt
     6121 agcaaggagg aaatccttga aagtgatatg cgagtggcta tcatgagcga gttgattgag
     6181 gctaggtatg aacaaggaat cagtcagaaa aagctggaag aagtcagtgg aataagccag
     6241 cctgtcatag ctaggatgga gacaggaaag actagtcctc agttggatac agtcttaaaa
     6301 gtcctagcca gtttaggaaa gacactagca atcgtcccac ttgaacaggg aaaaagttga
     6361 ctagaatcag ttatagtctt ttctaataac aggtcatagt cacttataag aattactaat
     6421 aacatgtttt agtattctaa ttggaatata gctgtaaagt aagaattaca aaggatttga
     6481 gacgtttgct tcaagtcttt ttcttttgtc cgaaacagat atatagtttc aaactatatc
     6541 aaaccctaat tttttacaat tatacagacg ctttcttttc tgttaagata gtttcaacaa
     6601 caaattttgg aggacacatc atgtcaacta caatcatcgg tttccctcgt ttgggcgaat
     6661 tccgtgaatt aaaatttaca actgaaaaat actttagaaa agaaatctca gaagaagaac
     6721 ttttagcagc agcaaaagac ttgcgtgcta aacactggaa cattgtcaaa gaaaaaggca
     6781 tcactgaaat tccatcaaat gacttttctc actatgataa cttcctagat gcggctttcc
     6841 ttttcaacgt ggtgcctgca tccgttcaaa acttggactt gtctgacctt gagcgctact
     6901 tcgctttggg acgtggttac cagggagaaa aaggggacgt tcgcgccctt ccaatgaaga
     6961 aatggttcaa cactaactac cactacatcg ttcctaaatt tgaaaaagac actcaagtaa
     7021 aattggctgg tcacaagatt ttcgatgagt tccaagaagc caaagaactt ggattgaaca
     7081 ctcgtcctgt tcttgtaggt cccttcactt tccttcaatt gtcagacttt gaagaaggcg
     7141 tgaaagcaga cgacttcgta gatagcttag tggctgctta ccaagaagtt tttgctaaat
     7201 tggctgaact tggtgcgact cgcatccaat tggatgaagc ggctcttgta aaagacttga
     7261 cagctgaaga aaaagctctc ttcttgaacc tttacaacaa actcttggct gacaaaaaag
     7321 gtcttgaagt cttgcttcaa acttatttcg gtgatgttcg tgacgtatac gctgaccttg
     7381 taaacttacc agtagatgct atcggtcttg actttgttga aggtaagaaa actcttgaac
     7441 tcgttaaagg tggcttccca gctgacaaga ctctctatgt aggtattgtc aatggtaaaa
     7501 acatctggcg caacaactac gaaaagagct tggctgttct tgagcaaatt ccagctgaaa
     7561 acatcgtttt gacaagctct tgctcacttc ttcatgtgcc atttacaact gctaatgaag
     7621 aatttgaacc agcactcttg aaccactttg cctttgcagt tgaaaaattg gatgaaatcc
     7681 gtgacttgga tgctatccga aatggtcaag gttcagaagc tcttgcagcc aacaaagaac
     7741 tctttgcgac tgagcgtgtt ggtgaaaatg ctgaacttcg tgcgcgtatc gctggcttga
     7801 cagacgcaga ctacactcgt ttgccagcct ttgcagaacg tgaagctatc caagaagaag
     7861 ctttcaaact tccagctctt ccaacaacaa cgattggttc attccctcaa acaaaagaag
     7921 ttcgtgccaa acgtttggct taccgtaaag gtgaattgtc tcaaaaagag tacgacgctt
     7981 tccttgctga aacgattgac gaatggatca agtggcaaga agatattgac tttgatgtcc
     8041 ttgttcacgg tgaatttgag cgtaatgaca tggttgagta cttcggtcaa aacttgtcag
     8101 gatacctctt ctctaaaaat ggatgggtac aatcatacgg tatgcgtggg gtaaaaccac
     8161 caatcatctg gggtgatgtc actcgtctta accctatcac tgttaaatgg tctagctatg
     8221 cacaaagtcg tacaaacaaa cctgttaaag gtatgttgac tggacctgtt accatcctta
     8281 actggtcatt cccacgtgaa gacatctcta tcaaggattc aactctccaa atcgcccttg
     8341 ctatcaagga tgaagtactt gaccttgaag ctgctggtgt gaaaatcatc caaatcgacg
     8401 aggctgctct tcgtgaaaaa ttgccactcc gccgtagcga ctggtacgaa gactaccttg
     8461 actgggctat tcctgccttc cgcttggtac actcaacagt agcgccagac acacaaatcc
     8521 acactcacat gtgttactca gaatttacag atatcatccc agctatcgat aacatggatg
     8581 cagacgttat ttcctttgaa gctaaccgtt caaaccttga aatcttggac gaactcaaag
     8641 cgaaaaactt ccaaacagaa gtgggacctg gggtttacga tatccactca cctcgtgtac
     8701 caaatgaagg cgaaatcgac aacacaatcg aagccatcct tgctaaagtg ccaagcaaga
     8761 aagtttggat caaccctgac tgtggtttga aaacacgtgg tatcccagaa acaaaagaaa
     8821 gcttgatccg ccttgttgaa gcagctaaag ctgcgcgtga gaaattgtaa gattggattt
     8881 ctctccatac agtggttgtt caaaaagaaa tcaactagaa aaggttatta catatgtcac
     8941 gccaaacacc gtcactctca tttgaagtgt tccctccaaa cccagccgtg ggtaatgata
     9001 acattatttc agccttgcag gatatgcagg agttggctcc ccattttatc agtgtaactg
     9061 ccagcaataa taaatttaat atcaaggaaa cgacggttcg tttggctgac tttatccaaa
     9121 atgatttggc gattccgacc attgcccact tgccagctat ttatctaact aaggacaagg
     9181 ttgctgaaac tattgctgac ttggacaaag ttggggtgca gaaaatcttg gctcttcgtg
     9241 gggatattat tccagacgtg gaaccacaaa aggatttccg ctatgcaacc gacttgattg
     9301 agtttatcaa ggaacaaacc cctcactttg atattattgg agcttgctat ccagaagggc
     9361 atccagattc gccaaatcag atttcagata ttcaaaatct taagaagaaa gtggatgcag
     9421 gctgttcgag cctcgtaact cagctcttct ttgacaatga gcgcttctat gattttcagg
     9481 acaagtgtat cttggctggg attgatgttc ccattcatgc aggaattatg ccaattctga
     9541 atcgaaatca ggctctccga ctcttgaaga cttgtgagaa tatccatctt ccacgcaaat
     9601 ttaaagccat cttagacaag tatgagcatg accctgagtc gctcagagca gcaggacttg
     9661 cctatgcagt ggaccaaatc gtggacttgg taactcagga tgttgccggt gtgcatctct
     9721 atacgatgaa taatgctgat acagcaaaat acatccatca agcaacccat gccttgttta
     9781 atcaccagtc tctaggataa taaaagcaaa ccattcttct caggtgaggg gaatggttcc
     9841 tttttaatgg taaagacctc actttttaag aaaaatatga taaaatagat tctgtacgta
     9901 cttgatacaa agatgaaggt attaaataat acaatgcatt taaactgtaa gatttaacga
     9961 cacgagtgag tcaaatcgat gttatttgac gaaagagtta gtaaagcatt tagctaatcg
    10021 cccgatagcg attagcgtga aaggtttaga tactttaata ttaaaccttt ctaatgatgt
    10081 atcttgaaaa ctgaacacgc ctacaactct gtggaaaaag ataaatctgc ctaggagcag
    10141 tcgctccgtc gttcgatttc ctattttcct ttgagttgct tgacggctta gcatcttgag
    10201 taattgaaca cggccgaaaa gctgtgtaaa aaagataaac tgtcttgtct tcatcgaaga
    10261 cttcgtcagt ttcctatttt tactttgctt ttgacgtcct tggtatcttg atctttgtag
    10321 gcaaggcgta taatttcatc aatccaaagg ggattaaaat ggcaaaacaa gtgtttcaaa
    10381 cgacttttgc gggtcgtgag ttaattgtag agactggtca ggttgctaag caagcaaatg
    10441 gctctgttgt cgtacgttac ggtgagtcaa ctgtcttgac tgctgccgtt atgtctaaga
    10501 aaatggcaac tggggatttt ttcccactcc aagtcaacta cgaagaaaaa atgtatgcgg
    10561 ctgggaagtt tcctggtggc tttatgaaac gtgaaggacg tccttcaaca gatgcgacct
    10621 tgacagcgcg tttgattgac cgtccgattc gtcctatgtt tgcggaaggt ttccgtaatg
    10681 aagtccaagt catcaataca gtgctttctt atgatgaaaa tgcatctgca ccaatggctg
    10741 ctatgtttgg ttcatctttg gcactgtcta tttcagatat tccatttgac ggaccaattg
    10801 ctggggtaca agtgggatat gtagatggcc aaatcatcat caacccaagt caagaacaag
    10861 cagagcaatc tcttcttgaa ttgacagtag ctggaaccaa gcacgctatc aacatggtag
    10921 agtctggtgc caaagaattg tcagaagaaa tcatgttgga agcgcttctt aaagggcacg
    10981 aagctgtcaa agaattgatt gccttccaag aagaaatcgt tgctgctgtc ggtaaagaaa
    11041 aagcagaagt ggaattgctt cacgtggatg ctgaattgca agctgaaatc attgcagcct
    11101 acaacagtga cctccaaaag gcagttcaag tagaagagaa attggcccgt gaagctgcaa
    11161 ctcaagcagt gaaagaccaa gtgactgccg tttacgaaga aaaatatgcg aaccacgaag
    11221 aatttgaccg tattatgcgt gatgtggctg aaatcttgga acaaatggaa cacgcagaag
    11281 tgcgacgttt aattacagaa gacaaggtgc gtcctgatgg tcgtaaggtc gatgaaatcc
    11341 gtcctttgga tgcggttgtt gacttccttc ctcgtgtaca tggttcaggt ctctttactc
    11401 gtgggcaaac tcaagctctt tcagtcttga ccttggctcc gatgggagaa actcaaatca
    11461 ttgatggttt ggatccagag tacaagaaac gctttatgca ccactataac ttccctcaat
    11521 attctgtagg ggaaacaggt cgttacggtg cgccaggtcg tcgtgaaatc ggtcacggtg
    11581 cccttggtga gcgtgctctt gctcaagtct tgccaagctt ggaagaattc ccatacgcta
    11641 tccgtctagt agcagaagtt ttggaatcaa acggttcttc atctcaagct tctatctgtg
    11701 cgggaactct tgcccttatg gctggtggtg tgccaatcaa ggcgccagta gctggtattg
    11761 ctatgggact tatctcagat ggaaataact acacagtatt gacagatatc caaggtttgg
    11821 aagatcactt tggagatatg gacttcaagg ttgcaggtac tcgtgatggg attacagccc
    11881 ttcaaatgga tatcaagatt caagggatta ctgcagaaat cttgacggag gctcttgctc
    11941 aagccaagaa agcgcgtttt gaaatccttg atgtcattga agcaaccatt ccagaagttc
    12001 gtctagaatt ggctccaact gctccgaaaa ttgatacgat caagattgat gtggacaaga
    12061 ttaagattgt catcggtaag ggtggagaaa ccatcgacaa gattatcgct gaaacaggtg
    12121 ttaagattga tatagacgaa gaaggaaatg tgtctatcta ctctagtgac caagatgcta
    12181 ttaaccgtgc caaagaaatt attgctggtt tggttcgtga agccaaagtg gatgaagttt
    12241 accgtgctaa agtcgttcgt atcgagaaat ttggtgcctt tgttaacctc tttgataaga
    12301 cagatgccct tgttcatatc tctgagatgg cttggactcg taccaatcgt gtagaggatt
    12361 tggtagaaat cggggatgaa gttgatgtta aggttatcaa gattgatgaa aaaggccgta
    12421 tcgatgcctc tatgaaggct cttctacctc gtccgccaaa acctgagcat gatgaaaaag
    12481 gtgaaaagtc tgagcgccct caccgcccac gtcatcacaa ggattacaaa cctaagaaag
    12541 aatttacaga aacaccaaaa gattcagaat aagaaaagga gaaatgtatg gggtggtggc
    12601 gcgaaaccat tgatattgta aaagaaaatg atccagcggc ccgcaccact ttggaggttt
    12661 tgctgactta tccaggtgtc aaggccttgg cggcccaccg tctctcgcat tttctctgga
    12721 agcacggctt caaactatta gctcgtatgt acagtcagtt ttggcgcttt tggactcaga
    12781 ttgagattca tccaggagcc cagattgatt caggtgtttt tattgaccat ggttctggtc
    12841 tggtgattgg agagacagcg attgttgaaa aaggcgttct tctctatcac ggagtgactc
    12901 tcgggggaac agggaaagac tgtggcaaac gccatccgac tgtacgaaag ggagccctca
    12961 tatcagccca tgcccaagtt atcgggcctg tggaaattgg tgaaaatgcc aaggtcggtg
    13021 ctgcagcagt tgtcgtagca gatgtaccta gtgacgtgac ggttgtcggt attccggcca
    13081 agattgtccg tcttcatggt aagaaagatg agcctgtcat tcacgaagtc gaagaaaaac
    13141 gagagtatta tgtcaataaa ctcgagcagg ctaaagatgc cagtcacaga tcgtctggtt
    13201 tgtagaggat acctatatga ttcaagcaag aaacaagtta agccaagagg agctatctga
    13261 ggcgaaaaaa gtaattaact gttgccaaaa ctatgacggt acctatcgcg atccctatct
    13321 ctctaacatg cttaattttg acccaaacat gcccgccttt ttcctttatt atgaaaaagg
    13381 cgaacttgtt ggtttattaa ctgtctatgc agatgaccaa gatgtagaag tgacgatact
    13441 ggttcatcca ggtcatcgcc gtcaggggat tgcgcgtgca ttgtttacta gttttgagag
    13501 agaaacagct tctttcccca ttcggtcagt cacttttcag acagaacgta tttttttaga
    13561 gaatcatcct gactttgcca gtaactgggg actaatcgag gatgaagaga cagaaacttg
    13621 gttaggtaag gatagaagac cttatccgtt agcaaatgtt tcccatcttg aaattttgtt
    13681 agcagatagt tcgtatcagg atcaaattag tcaattaaaa tttcaggcat tttcagagga
    13741 acatgaatcg agagaagttg tggatagata tgtcgctgaa gctctgaaag atccagaaag
    13801 ccgactatat attttattaa aagctggtca ggttattgga acttgtacgg ttgatttatc
    13861 gactaatacg aattacctct acggtttagc aatattggaa cctgaacgtg gaaaaggcta
    13921 tggaagctac ttagcaaaat ccctcgtcaa ccaactaatt gagcaaaatg acaaggaatt
    13981 tcagattgca gtggaagata gcaatgtagg tgccaaacgt ttgtatgaaa aaattggctt
    14041 tgtcaaacag actcaggtgg tttatctgaa tgagaaagga gcaagggatt ccgaagtgta
    14101 gagatattcg gactgaaatt tacttgaact tttagtgatg aaactaattg ttcttggatt
    14161 tcagctttcc tgattatgat ttatgattaa aatctatgac accatgtctc gtgatttgcg
    14221 agaatttgtc ccgattgagg acggcaagat caagatgtat gtttgtgggc caacggtgta
    14281 caactatatc cacgtgggaa acgcccgttc gacagtagct tttgatacga ttcgtcgcta
    14341 ttttgagtac cgtgggtata aggttgccta tatttccaat tttacagatg tggatgataa
    14401 gattatcaac cgtgccaggg aagaaggcat cacgcctcag gaggttgcgg ataagtacat
    14461 cgctgccttt cgtgaggatg tgacggcctt aggcgtgaaa cctgcgactc gccatccgcg
    14521 tgtagtggag tttatggcag acatcatccg ttttgtggaa gacttgatcg agaaaggctt
    14581 tgcctatgag agtcaagggg atgtctattt ccgtgtagaa aaatcccaca actatgctaa
    14641 attggctaat aaaaccttgg aagatttgga tctaggtgct tcaggtcgta ccgatgaaga
    14701 aacggctcgt aaggaaaatc ctgtagattt tgctctatgg aaatcttcca aaccaggtga
    14761 gatttcttgg gatagtcctt ggggacctgg acgtccgggt tggcatattg agtgttcagt
    14821 catgtcgaca gagattttag gtgataccat tgatatccac ggtggtggag ctgacctaga
    14881 gtttccacac cacaccaatg aaattgccca atctgaagct aaaacaggca aggcttttgc
    14941 taactactgg atgcacaatg gctttgtcaa tatcgacaat gtcaaaatgt ctaagtcctt
    15001 gggtaacttc attacggtac acgatgccct taaaactctt gatgggcaag tacttcgttt
    15061 cttctttgcg acccagcact accgtaagcc tatcaacttc acggaaaagg cagttcgcga
    15121 tgccgagacc aatctcaagt atctgaagaa tacttacgaa caaccattta caggaaacgt
    15181 ggatgcccaa gagttacaaa actttaaaga taagtttgta gcagctatgg atgaagattt
    15241 taatgctgcc aacggtatca cagttgtctt tgaaatggcc aaatggatca actcagggaa
    15301 ctatgatgca agtgtcaagc aagctcttgc agatatgtta gaaatttttg gaattgtctt
    15361 tgttgaggaa gttttggatg cagagattga agacttgatt caaaaacgcc aagaggcgcg
    15421 tgccaatcga gactttgaga cagcagacca aatccgagac caactggctg ctcaaggcat
    15481 taaactattg gatacaaaag atggagtgag gtggacacgt gattgatgtc aatctcatta
    15541 acgggattgc gctagccttt gagggggatg cggtgtattc tatgtatatt cgccgtcacc
    15601 tcatcctcaa aggtatgacc aaacccaata aactccatca agaagcaacc aagtatgtgt
    15661 cagccaaggc tcaggctcgc ctgattgctc tcatgttgga ggagcaggtc ctaacggaaa
    15721 aagaagaaga aatctataaa cgtggccgca ataccaatag ccacacaaag gctaaaaatg
    15781 cagatgtcgt gacttatcgt atgtccacgg gatttgaagc ggttatgggc tatctccata
    15841 tgactgagaa tctggaacgt cttgagagtt tggtttcatg gtgcatccaa aaagtggagg
    15901 gctagaacat gagggcaaaa gaactacaag actggtttcc tgaggctcgg atttcagacc
    15961 aaccagtaga gaaagagggc tatctcacgc tccctttagc ttctcagcag tggattttgc
    16021 tggaggaagc tgggctcagc gagcgtgaaa agcagttggt tgcccttttg acccagcagg
    16081 agcaggctcg ttcgctaaac ccttggtatt cctatctggt tgaaggcaag ggacaggcac
    16141 cgcaagtttt taaaaagatt cagttggttt attgccatct ttcttatttt cagcaggaaa
    16201 atctggcttc ttggctagat atgatgcgga ctctttttcc gaattgtcag acagtgctac
    16261 aggtcggagc tcaggattat gttttcgtgc ttcaacaaga caaatacact tctgtaagag
    16321 atattttaag tgatacgatt gaagcggttg agtatgactt tggacttcgt ctttctatca
    16381 tgttgggtca ggtttggtct cagacaggac atcaagccct atcagactta atcaaagctg
    16441 agcgggattt gttcaagaca tggtggcgtc agggtcacca aggtgttcat actttttctc
    16501 agctctatct ttggagtatg ggagaaagac tcgtggactt gaagccaatt aaagaatgtc
    16561 tacaccagat gattttggat caagatcaga ttcaggaaat cattctctct ctttgggaaa
    16621 atagtgctgt tctcactaaa acagcccagc aactctatct gcaccgcaat tctctccaat
    16681 acaagattga taaatgggaa gagttgacag ggcttcagtt gaaagagttg accgacctga
    16741 ccttgtgtta tcaattgatt ttaggttctt tgtcaactat agttggtttg taaagaagtt
    16801 aatatttgga gaagaggatt gccatcttct ccatttttat gtgcagagtt atagtggctt
    16861 gatgctggga aagtacactg tgactgctaa aacattccta gaagttgctt tgatttacct
    16921 aatctattta tgcaaatttt atgttatttt actataacag ttgttgctaa gccaaataat
    16981 agtggggaag ttcatttaga cgtaagcatt gaagataatc agggaggtag tgggcataat
    17041 ttcagttctg tttcaagtag ctcacaaaca gctaaatatg aagaaactgg ttataataac
    17101 aattcatcat tatatataac gattgataaa acgtctgatg caacagctct tttgaaatta
    17161 aagttgaata atgttgataa tcaacctgct actgaagttc ctagttcagg aattactgta
    17221 aaattaaatg ctaaagataa tgctggaaac tggacaagtg cttcgaataa aaaagaaata
    17281 acagtaaaaa ttgtttctgc taaaccgaca tatccagaca aaatcttagt gaaaaatcct
    17341 gataatataa aagatacaga aaaaatgcca ttattgaaaa attgaaagag gcaaataaaa
    17401 atcatccagc aggagctcca acctttgcta aaggtgaagg agagcatgca aatgatattg
    17461 tagcaactta ttcagatggt acaacttatt atgtaccgtt aaatgatgtg acaaaatatg
    17521 cgaggtagtg gctgtaccac tcacttattc acctccccgt gatttgtagt agtgataggc
    17581 tttctcacta ttattataaa acaaaataaa gatcacaaca ctttttcatt ctgtgttgtg
    17641 ccttgagtga aacgaaagga atgaattata aatatgaaaa gtatagtcac tagcatagat
    17701 gagcgcttgc gtactcgcct acgagtgatt atctggaagc aatggaagaa gaaatcgaga
    17761 cgattatggg gattgcttaa gttaggggtt cctaaatgga tagcagataa ggtatctggc
    17821 tggggcgacc actatcaatt atagtaaaat gaaataagaa taggacgaat cgttcaggac
    17881 agtcaaatcg atttctaaca atattttaga agtagaggtg tactattcta gtttcaatct
    17941 actatagtag ctcagaagtc ggtacttaaa cgtgctatat caaaaccagt ccttgaaaaa
    18001 cgtggactgg tttcgtgttt ggattattac cttgaacgac atgcgttaaa agttagttga
    18061 accgccgtat gccgaacggc acgtacggtg gtgtgagagg ggctagagat tatcccctac
    18121 tcgatttcga aatctagtgg aatgaatctg gaatagtcca tcgagctttc taata
//
LOCUS       DAVLRT010000037        17398 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLRT010000037 DAVLRT010000000
VERSION     DAVLRT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.037"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(446..1030)
                     /gene="pdxT"
                     /locus_tag="VXM85_001243"
     CDS             complement(446..1030)
                     /gene="pdxT"
                     /locus_tag="VXM85_001243"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1345820.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEDVEILATVDNQIVA
                     AQEKNMLVTSFHPELTDDAHLHQYFIEMCKEKRQ"
     gene            complement(1031..1906)
                     /gene="pdxS"
                     /locus_tag="VXM85_001244"
     CDS             complement(1031..1906)
                     /gene="pdxS"
                     /locus_tag="VXM85_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1345821.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(2058..3437)
                     /locus_tag="VXM85_001245"
     CDS             complement(2058..3437)
                     /locus_tag="VXM85_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1345822.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            3731..4654
                     /locus_tag="VXM85_001246"
     CDS             3731..4654
                     /locus_tag="VXM85_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1345823.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            4714..5319
                     /locus_tag="VXM85_001247"
     CDS             4714..5319
                     /locus_tag="VXM85_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1345824.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQSFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEEFDWENL"
     gene            5337..6581
                     /locus_tag="VXM85_001248"
     CDS             5337..6581
                     /locus_tag="VXM85_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1345825.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGDHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(6698..6955)
                     /locus_tag="VXM85_001249"
     CDS             complement(6698..6955)
                     /locus_tag="VXM85_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1345826.1"
                     /translation="MDPKKIARINELAKKKKTEGLTLEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(6997..9033)
                     /locus_tag="VXM85_001250"
     CDS             complement(6997..9033)
                     /locus_tag="VXM85_001250"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1345827.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(9245..10162)
                     /gene="glyQ"
                     /locus_tag="VXM85_001251"
     CDS             complement(9245..10162)
                     /gene="glyQ"
                     /locus_tag="VXM85_001251"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1345828.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(10355..11116)
                     /locus_tag="VXM85_001252"
     CDS             complement(10355..11116)
                     /locus_tag="VXM85_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1345829.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(11389..12231)
                     /locus_tag="VXM85_001253"
     CDS             complement(11389..12231)
                     /locus_tag="VXM85_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1345830.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(12345..13736)
                     /locus_tag="VXM85_001254"
     CDS             complement(12345..13736)
                     /locus_tag="VXM85_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1345831.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(13971..14348)
                     /locus_tag="VXM85_001255"
     CDS             complement(13971..14348)
                     /locus_tag="VXM85_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1345832.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            14563..15540
                     /locus_tag="VXM85_001256"
     CDS             14563..15540
                     /locus_tag="VXM85_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1345833.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            15537..16619
                     /locus_tag="VXM85_001257"
     CDS             15537..16619
                     /locus_tag="VXM85_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1345834.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(16683..17398)
                     /locus_tag="VXM85_001258"
                     /pseudo
     CDS             complement(16683..17398)
                     /locus_tag="VXM85_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         5114 a         3838 c         3164 g         5282 t
ORIGIN      
        1 atttcctctg gatctcgaat aaaggtcttt agcgaactaa gaattttttc tattaccata
       61 atagtatcat aaatgagact tgattgaaag cgtaaacata gctttgttaa gtgatacaac
      121 tattctatac aaaatttaaa cataaaactt aggtatactt aataaatgag aatttaagtt
      181 agctataaca aagatagtag catatgtaaa actctaagca ggcagagcct gcttagagtt
      241 ttacaatcga atttcccaaa gaggaagcaa gatgaaagcc gtcattgctt gtgctcataa
      301 gattctcaga atcgtttaca aactactctc caccaagcag acttatcaaa aagaaaaggc
      361 gctaggactg aggaaacagt tctaacgcaa aaactaaaaa aatttcaatt acagtatagc
      421 acaggaagtg attttttgag cttttttact gtcttttttc tttacacatt tcgataaagt
      481 actggtgtaa gtgagcatca tctgtcaatt ctggatgaaa agaagttacc aacatatttt
      541 tttcttgggc tgcaacgatt tgattatcaa ctgttgctag aatttctaca tcttctccaa
      601 cactgctgat aattggacca cggataaagg tcattggaat cttgccaact cccttacatt
      661 ctgcttccgt gtagaaactt cctaattggc gcccataagc attacgctcg accaccatat
      721 ccatagttcc tagatgactc tctttctgag aagtgatttc cttagccagc aaaattaagc
      781 ccgcacaggt cccaaacact ggtaagccag atagaatggc ttctcggatg ggaagtagca
      841 tgttctggtc acgtaagagc ttgcccatgg ttgtagactc accaccaggc aaaatcaaac
      901 ccgacaagtc actctgatct tgctgaaaat catctagatt tctgagttct acactctcga
      961 cacctaattg atctagcact tttgcatgtt ctgcaaaggc cccttgcaag gccaatattc
     1021 cgattttcat ctattttcct cgttcagcca tgagaatttg gatttcattt tcattaatac
     1081 caaccatggc ttctcctaaa tcttcagaga tttgagctag gatttgagga ttacggaagt
     1141 tagtcacagc cttaacaatg gcactcgctc gtttaacagg atctcctgac ttgaaaatac
     1201 ctgaaccgac aaagaccccc tctgccccta gttgcatcat taacgcagca tctgctggcg
     1261 ttgcaacacc tccagcagcg aaatttacaa ctggcaattt tccatgttca tgaacatatt
     1321 ggaccaattc tacagggact tgcaaatcct tggcagcaac ataaagctcg tcctcacgta
     1381 agttttgaat gcggcgaatt tcctgattca tcatacgcat atgacgaaca gcttggacga
     1441 tatcccctgt ccctggttct cctttggtac gaatcatgga agcaccttca gcgatacgac
     1501 gcaaggcttc acccaaatcc ttagcaccac agacaaaagg aacttggaat tctttcttgt
     1561 ccacatggaa acggtcatca gctggagata gaacttcact ctcgtcgata taatcaattt
     1621 caatagcctc taaaatctga gcttcaacaa aatgcccgat tctgacctta gccattactg
     1681 gaatactaac cgcttcttgg atttccttaa tcatctttgg gtcgctcatg cgggaaactc
     1741 ctccagctgc acgaatatca gccggaattc gttccaaggc catcacagct gccgcaccag
     1801 cagcttctgc gatacgagcc tgttcaggat tctgcacatc cataataaca ccacccttga
     1861 gcatctgtgc caagttttta tttagttcat aacgattttc agtcatttgt tttatcctca
     1921 tcatttgtat tggtagctac cttttcattt taagtcagaa cagaatgaag cacaagataa
     1981 aaaaactata attgtatggg gacagattag ctaaaaacta tctgacctta acttaaggcc
     2041 agatagctca ttcattttta tttttcagcc gtaagggcag ccattgtgat gtagttgtat
     2101 ggtttgttga agtgtggcaa gaagaagagg tctgtcaatg ccaatttatc aattgtcaca
     2161 tgctcttgga tagcaagtga gaacatgtgg attcccatgc taattgcaat atcatgggaa
     2221 accatttggg caccaagaat ttcacggcta tctttgtcaa agacaatctt aattgctact
     2281 tcatggttgt catgtttcat gaattctggt ttttgaagat cgttaaagcc tgtttcagtt
     2341 gcgttgtaac cagcagcttt cgctttttca agagtcaaac cagttgaaac catgtgaaga
     2401 ccgtagattg agataccatt tgaaccttga acaccgattc cttccaattc atgtccacaa
     2461 gcgttgtagg caccaacgat accagtgcga acagcatttg aagcaagagc gatatagctt
     2521 gtatctttac gagcattgtc ataaacagtc gcacagtcac caacagcgta aacgcctggg
     2581 atagatgttt cttgtttctt gtctacaagg aaggcaccgt tgcggaagag ttcgatctta
     2641 ccatcagcaa gggctgtgtt tggacggaaa ccaactgcaa ggataaccat atccacgtca
     2701 aagctttctt tgtcagtaat caagcgttca actttaccgt caccttcgat tgctttaaca
     2761 gtttgaccta gagccaagcg gatgttgtga tcttccaagt tcttcgccat catttgtgtg
     2821 aagtctttgt catagtaacc gttcaagaca gtatcaacga tatcaacaag gacaacttct
     2881 tttccaagac gttcaaaggc ctcagcaagt tcaacaccga tgtaaccacc accaacaacg
     2941 gcgatacggt cgaggtgttg gctcttgtca gaaagtttat tgataacttc ttcagcattt
     3001 tggtacaatt tcacgaattg aacattttca agagttgctt taaattcgcg gtttccttta
     3061 acaatttcaa caccttcgat tggtggcaag attggtgtag aacctgtagc gaaaatcaat
     3121 ttttcgtatg attctttgtg ctcttttcct tcaacttccg ctgtaactac tttgttatca
     3181 tagtcgattg aaagaacagg tgagttcatg taaactttag cacctttagc ttccaatttt
     3241 tctttatcag aatagaacaa gccttcagca ccgtcaattt gttcaccaat ccaaagagcc
     3301 attccacatc ctaggaaaga gatgttagag ttttggtcaa atacaacaat ttcgttctca
     3361 tttccaaaat tatccaacat ggtattgata catgctgtac cagcgtggtt agcaccgact
     3421 acaacgattt tactcataaa aatttcctgc cttcattcta aatttacctt actagtatac
     3481 catttaccgt tagcgtttac aaggggggta ctattatttc agcatttttt atcaaaaata
     3541 attccccatt tatcctgttt tcaagcctca tgaattttat gagcatattt cttgactttt
     3601 tccaaatttt gtttgtgaaa agtctaactt tatggtatta taataacatg aaaataaaat
     3661 gtaagggctt caatttagat gattatttga tattacaact tattttctat aagagaaagg
     3721 agttggtagc ttgactcttc gttcacattc tgaacggcta atggggacta ctatcactat
     3781 ttcattagtg gatgagcaag ccgatatctt tctccaaaaa tcctttgact tgctcaaaga
     3841 gcttgaatac cgcttcaacg ccaatagtca agaatctgag ttgatggaaa tcaattatca
     3901 agcaggaata tcccccgtca cggttcatcc agacctgttt gagctgattt cacttgggtt
     3961 agagcatagc ctagcgctct ctagccacct caatatcagc attggtccct taattcaaac
     4021 ctggcgtatc ggtttttcag atgccaaggt cgcccagcct caagaaattg aatcggtgct
     4081 gcctctaatc aatcctcatg gtatcgagtt agattcttct acttctactg tgtttttaaa
     4141 acagaaagga atgaagatcg atctaggttg tttagccaaa ggatacagtg cggataaggt
     4201 tgcccaattt cttaggaaag agggggtgac ttctgccttg atcaatctgg gagggaatat
     4261 cctgaccatt ggaaaaaatc aggcaagagg ggataaccca tggcaaatcg ggattcaaga
     4321 cccagccaat cctaggggaa atcatttaat gaccatccct gttgtcaata aatctgtcgt
     4381 gacttcaggc atttatgaac gtcacctgac cgtcgatgga caagattacc atcacatttt
     4441 tgacagtcaa acaggatatc ctgttgaaac ggaactagcg agtctaacaa tcatctctga
     4501 taaatcagtc gatggcgaaa tctggacgac tcgactcttt ggagaaagac ctgcttctat
     4561 cctctggcaa gtcgaaagtt tggagggcat cgaagttatc ctcatcgata aagaaggcca
     4621 cctaagctgt tcttcaggaa ttcctactct atagagacaa ctgtttcaat ggaattttaa
     4681 aattgtatta tgtaaaaaga gaaaggaaat ctcatgttaa aacttattgc tattgttgga
     4741 acaaattcaa aacgttctac aaaccgtcaa ttgcttcaat acatgcaaaa acactttgct
     4801 gacaaagctg aaattgagct tgttgaaatc aaggccattc ctgtcttcaa caaaccagct
     4861 gacaagcaag tacctgctga aatattggaa attgctgcta aaatcgaaga ggcagatggc
     4921 gttattatcg gtactcctga gtatgatcac tctattccag ctgttttgat gagtgctctt
     4981 gcttggctat cttacggtat ctacccactt ttgaacaaac caatcatgat tacaggtgct
     5041 tcttacggta cgcttggttc atctcgtgcc caattgcaac ttcgtcaaat cttgaatgct
     5101 cctgaaatca aggcaaatgt tcttccagat gaattcttgc tctcacactc tcttcaatca
     5161 tttaacccaa gtggcgactt ggttgacctt gatgttatca agaaattgga tgccatcttt
     5221 gatgacttcc gtatcttcgt gaagattact gagaaattgc gcaatgcgca agaattgctt
     5281 cgcaaagatg cagaagaatt tgactgggaa aatttgtaag ataggagacc gaaagaatga
     5341 aatttgttgg acttgttgga tcaaactacg atcaatcata taaccgcaaa ctcttggaat
     5401 ttatccgtcg caatttcaaa ttcaaatttg aattagaagt acttgaaatc gacgaagttc
     5461 caatgtttaa ccaagacgaa aaatgggacg aaagcttcca attgcgtttc ttgtataaca
     5521 agattacacg tgctgatggt gtcattatcg ccactcctga gcacaaccac actatctcag
     5581 cttcactcaa atctgtactt gaatggcttt catacgaagt tcatccattt gaaaacaagc
     5641 ctgttatgat tgtgggtgca tcatactatg accaaggaac atcacgtgct caagttcacc
     5701 ttcgtaaaat ccttgatgct ccaggtgtta atgcctacac gcttccaggt aacgaattcc
     5761 ttcttggtaa agctaaggaa gcttttgata acaacggaaa tatcaccaac gaaggcactg
     5821 ttaaattcct tgaaacttgc ttagataact ttgttaaata cgtaggagtc gtttcgaaat
     5881 tgaaaaaacc aaaaccaatc gaatcagaag acttggattg tggaaaacca atcgctacaa
     5941 ccatcacaga agttgaccct gacgatccag aatgggtaga aaaagttgca gcaatcactg
     6001 gtgctgtttc tggtgatacc tatgtcaaat tggaccacgg tatcctaaca gttaaccaaa
     6061 ttgatatgtt cttgaaagct atgccatttg aattgacata cgctgacgac aacaaccaat
     6121 tcctctacta caacaacgct caccaagatc cagacactat gttcgctaaa cgtgtaccac
     6181 ctcaatcagg tagccgtatg tcgactgttc atggttctct tccaccagca cgtatgaaga
     6241 acgtagagtg ggttatcgga acacttcgca acggaaacca agaatacgtc cgtacgatcg
     6301 ttccaggttc tcctgcaggt gttatcaaca ctcacaacta ccaagcaatg tactatcctg
     6361 atggatcata cgctggtatc aatgaaattg tctttaactt ccaaccatgg cttgactggt
     6421 acctaaaaga gactggtcaa cgtttggtgg gtggtagcgg accatttgct cctgctgccg
     6481 gagatcatgg agacgccgat gctacttctg gcgcttctga ttcaggggat gctggaggcc
     6541 acggtggtga cgcagacgct acagccggtg caagttacta atgatagttt ttaggaacca
     6601 ttatggttcc ttttttagcg aaaaaagact agcaaacaca aactgctagt ctctttgtta
     6661 tcgtttcatc tatctttgtt aaaaaaaaga gtacatatta tgaatttggg tcatcaagac
     6721 tacggccatg taatcctttt tcacgttgta cttggcgtag tttttctggt gtaacatcgt
     6781 ttccttcttc gtccacaatt ttgattcctt caatgtgatg acgaacagcg cgacgataac
     6841 cttcgatgta ctcctcacgt agtttggctt gttccacttt ttcttctagt gttaagcctt
     6901 ctgttttttt ctttttagca agctcattga tacgagcaat ttttttcgga tccatttctt
     6961 ctcctctgta ataagataaa gtccgtttat caaattttat ttagtgttaa tttggttaaa
     7021 acaagcaaac ttagctgctt tcttggttag ttgtgacaag attgccaaac gattttgacg
     7081 gacagcctga tcttcagcca ttaccatagt attttcaaag aaagcatcaa tgactgggct
     7141 aagcgcaaag agttgtttca attgctgact tgcaggtcct gataaaacga gtgtttctac
     7201 tgcttctgcc aaagcttttt cttggtcatt ctcaaatagt gctgaatcaa ccgtagcaac
     7261 cccttctgcc ttctcagcca ggttaaaggc acgagaaagt gattcaacag atggtttaaa
     7321 atcttcttcc ttgcttactt ctacgagagc acttgctgct tccaacatat ctgccacaac
     7381 aaagtttgaa cctgcaagaa ctgcttcctt gatatctttt ggagtagagc ccatcatctt
     7441 atcaacacga gccttgataa agtccataac ctctgcttta ttttcataag tcaaactgtc
     7501 aaatttcaat gcataaaggc tatcaatcag ctcatccata gcaatgtgcc aaccaaaggc
     7561 atccaagata cgaaccacac cttgagttgc acgacgaagg gcataagggt cattagaacc
     7621 tgatggaatc aatcctactg agaagaaact caaaatcgta tccaatttgt ctgcaatggc
     7681 tagaactgcg ccgaccttgc tctctggaag ttctccttca gctgatgtag gcatgtagtg
     7741 ttcacgaata gcagctgcca ccgctggagt ttcaccagca agaagggtgt atttttcacc
     7801 cataattcct tggagttcgt caaattcacc aaccatacct gtcaacaagt caaacttgta
     7861 aatggctgct gcacgagcaa ggtcaactgt ttcatccact gacaaactag ctttttctgc
     7921 caaaagtaca gtgatttgac ccgtacgaat catgtgttca cgaagggaac caatcttctc
     7981 atggaaggtg acattgttta atttttcaac aagatctgaa atcaccaatt tttggtcttc
     8041 acgccagaag aattctccgt cttccaagcg ggctaccaag actttttcat ttcctttgat
     8101 gacatttttc aaacgctctg cgtttccgtt acgaacagaa atgaagtttg gcaagagttt
     8161 tccatcttga tcacgaacaa caaagtaacg ctggtgttcc ttcatagaag tcaccaagac
     8221 ttcttctgga acttcaaggt atttagcatc aaaacttccc atgaaggcag ttgggtattc
     8281 aaccaaattc aagacttcat tcagcaaatc cgcatcaatt tcgatacgta caccatgttt
     8341 tgcctcaatt tccttgattt ggtcaacaat catttgctca cgttcacatg gatctgcgat
     8401 tacaaactgc ttacgaaggt cttcttcata gctcaatgct gactgaattt tggtttcttt
     8461 ccccaagaaa cgatggccac ggctcacacg acttcccttg atatcaagga aatccaagtc
     8521 aaactcttgc tcatccaaga gaacagttaa agtgtgaaca gggcggatgt attcaaagct
     8581 atttccagcc cagtgcatgc tgacagggaa agtcaatgac ttcaagacat ctacaacacc
     8641 aggaacaatg gcttcaactg cttgaccaac ttcttcctta gtgacataga catattcttc
     8701 acccttgatt tcacggaatt cgatatcttc aacagtcaag ccttttccac ggacaaatcc
     8761 ttgagctgct ttggtgaagt ttccatcact atccaaggca attttctttg ctggtccctt
     8821 gaaatcttct gtcaaatcag actgtttgtc tgataaacca gtcacacgaa cagccaaacg
     8881 acgtggtgtt gagaaggttt gaatggcttc aaaagacagg cggttttcct tgaggaaggc
     8941 tgccattttt tcgcctagtt gtttttcact tggtgtgaca acataggctg gtaattcttc
     9001 aagaccgagt tctactaata agttttttgt catgtttttt ctccgaaaag tatcttttta
     9061 ttttccagtt tgccttatgt gattggcacg caagcaacca acttcgttgt ttcattgcta
     9121 aaattggagc caaaagtctc caattttgcc tagtatcctt agtttcacta aggatacctt
     9181 catcactgcg gcaactgact cagggctcac tctgttcgcc cattttcgta atttgtcact
     9241 ctctttattc tgcgtcttct gctaggagtt tagctcttgt ttcttcatcc aaaagtgggt
     9301 aacctaggcg tttgcgttct gcaacaaagg ttttggctac aacacgggcc aagttacgga
     9361 tacgagcgat atagcctgca cgctctgtta cagatacggc accacgcgcg tcaagcagat
     9421 taaaggtatg tgaacatttg agaacatagt cataggcagg gtgtaccaag ccttcttcta
     9481 atgcacgacc agcttctttt tcaaacttat caaagttttc aagcaacatt tcttggtccg
     9541 aaatttcaaa tgaatatttt gagtgctcat actcaggctg gataaagatt tctccgtatt
     9601 ttacaccatc agcccactcg atatcataga cagagtctac ttcttgaatg taagaagcca
     9661 agcgctccaa accataggta acttccgcag tcacagggcc agttgccaat ccaccgactt
     9721 gttggaaata agtgaactga gtgatttcca ttccgtcaag ccaaacttcc caaccaagac
     9781 cagctgaacc agttgatggg ttttcccagt tgtcctcaac aaaacgaata tcatgctcca
     9841 aaggattgat tcccaatttt tccaaagact caaggtaaag ttcttggata tttgatggag
     9901 aaggcttcat gaccacctgg aattggtggt gttggtagag acggttaggg ttttccccat
     9961 aacgaccgtc agcaggacga cgtgatggct ctacataagc tgcattccat ggctcaggtc
    10021 cgatagcacg aaggaaagtg taaggactca ttgttcccgc acctttttca ttatcataag
    10081 cctgcataag catacaacct tggtcattcc aaaattgttg caaagtaagg atgatttcct
    10141 ggaaagtcaa tttcttagac attgtttact cctttttata taaattactt gcgacatgag
    10201 tctgcctcgt cgatcatacg aggcaagacg agttagtact gcgtctagct caggcactct
    10261 agtgctgagc gtggggcagt ccgactgtaa ggaggtttct gccactgacg cagtgaaatg
    10321 tcaatttctt agacattgtt tactccttta gtttttatat ttctttttca attaatttct
    10381 gctgaacaaa ccaatctaac aagggaaaag ttttttctgt atttgcccat aaatgataag
    10441 aatctaagac cgcttgtaca ctacctaaat tttctaccaa cttatctact gtcaaaaagc
    10501 tgcgccagta ttcataaaaa atacttgcat agttcttatc ataagtcgaa gaaccaaaat
    10561 cattcaatga atgccaacca tacttcttct gaaaaagttc tacgagagat tgattacaaa
    10621 ttttttccgc ttgaaattcc tcttctgtca agaaatactt gcgactaata tattcaacca
    10681 tcccctcttc gaaccagata taagagtcat aaccatcaaa atcatctaaa aaatgctccg
    10741 accaatgagc taactcatgt cctacaatct gtaagaggaa attttcagat aaagaatgat
    10801 agtgactttc tattgcttga gtttgctgag aacactcgta gtccatcaat tgatacaaat
    10861 acaactcttt ccaaacagct aaatcaggcg ccataaccat tcgtctatta tttgtatagg
    10921 ctggaacagc gctttcccta ataatctgtg tagcagcatt aaaatctgac caaataacgg
    10981 cttgcggtaa atcacagact gcaagttcat tctttaaaaa agattgataa tctttcaact
    11041 tttcagtatt tcttactaca aaatcacgaa atgtagctag ttgactatcg tcttttacaa
    11101 gataaaggtt gtccacggct ctcctttctt tcacacaata gactgaataa gcaaaagaac
    11161 cgcgtccgac aatcctaggt tgatttaacc taggggcgtc agaaacgcgg ttccacccta
    11221 atttattact tcattgtttt tgaaaatact acagaaagcg ccagctcatt attttgccct
    11281 acttggctcc cactatccca agctcgcttg aagaacgcca taagactttc tttcctgctg
    11341 actattatat caaaaattag aataatgtac aattcttttt atgattttct agaaatccac
    11401 atcatcaact cgtggagcac ccgattgaac agcaatcgtt tttaagtttt ctctctcctc
    11461 atgactcagt tcaattccaa agcaatcaag attagcttga atacgagagg ttgtgacaga
    11521 ttttggaagt ggtaaaaatc cttctgccaa gctccaggcc aaggctatct gagcaatcga
    11581 ttttccgtga tttgctgcga tttcttgcac ttgcttgcta tcaaatagtt ctccctgacc
    11641 aaaaggcccc caagcttcca ataaaattcc cttttcacga cagtaagcta cgacttgatc
    11701 ttgatacaca cctggcgcca agcgaacttg attgaccgca ggaacgatag ttgcagtttc
    11761 aagcaaggca tccaaatgat ggggaagaaa attgctaacg ccgatagcac ggattttccc
    11821 ttcttgatag aggtcttcca tcgctctcca aacttccgca ttgcgagttt tccatgcgtc
    11881 attttctctg agcggttttg ggttcggcca atgaatcaaa tacaaatcca aataatccaa
    11941 gcccagtttt tctatagatt tttccaaagc ttgacgagtt tgctcatagg tttgctgact
    12001 attccaaagc ttggtagtta cgaacatttc ttcacgtgga actccgctat ctttgattgc
    12061 ttgaccaaca ctttcttcat tctgataaat cgccgccgta tcaatatgac gataaccagc
    12121 cttcaaggct tctaacactg cacgataggc ttcttctcca tccttagcct taaaagttcc
    12181 aaatcccaat actggaattt ctactccatt atttaattga taggtattca tatgcttctc
    12241 cttctatttt attcatcaat tatagcataa aacatttttt aagtagtact atcttctgta
    12301 aaaaataagt ctgtcaaata tctaacagac ctattttttc ttgcttattc atcacgacta
    12361 tagtacagct catgagcttt taggcgagta ttgagcatct ctggtatggt cacaaaggta
    12421 taaccttgat ttttcaaata ctcaatgacc cttggcaagg cattgactgt cggactgtga
    12481 atatcatgca tcaaaacgat agagccgtta gctacttgat gctgaatttc tgtcaaaata
    12541 gatgcttcat ttttactctt ccagtccaga ctatccacat cccacatgat aaagctcaaa
    12601 tccaagctat tgcgaatatc atctgtaata gcaccataag gtggacgcat gagtttagaa
    12661 ctagaaccca gcactttagt tagcacatcc tcagtatcag taatctgctt tttagcttca
    12721 tcaagagaga gttgcgagag aatcggatgg ctccaactat ggtttccaac aacatgacct
    12781 tcagatttta tcctcttcac caagtcctca ttcccagaaa catttttccc aagcacaaag
    12841 aaagtcgctt taatatcata tttagctagg gtctctaata cctgcggggt cgttgctgga
    12901 tttggaccat catcaaaggt tagagcgaca actttttgat atttcttatc aaagtaagat
    12961 tggtacaagg ccgcatcttt ttcgagtaag tacgaagatt ggataacatc aaagaaagca
    13021 gatactggca aggctatctc ttctaaattt tcaaccacag gacttggata aaggataatc
    13081 tgactatcct tgtaatcaaa attccatgca gacaagtctt ggtcagagaa gttttttaca
    13141 atctgctcac tctggtcttg ctctattttt ttatcctcta tgaaggaggt caactctttt
    13201 atcagctgtt ccttagcctt actagcatct gaaaacagtt ggtcaagtgt aaaaggttgc
    13261 ccatcttctg ttaagtgaac ctttcctaga ctggtctttt cagtctcttc aatcttaaaa
    13321 gcaactaaat catagatttg tttggtcaca ttacgattaa cgactccctt taaacctgac
    13381 tcttcttgct ctgtatagta gaaaacaaga ttgtccttac tttctagctt gtccttaaca
    13441 tcttgattta tcagctccct aacagaggaa atcactttct ccccttggag aggataatag
    13501 gcaatcactt cggcttgccc ctgacgaaaa tgctccttct gatttccctc actcaattga
    13561 tcatctttct cttttttgag cgattcaatc ttttgctcaa aacttttttg ctggtagatc
    13621 ttaaaagcaa taaatccgcc taataggcaa atagaaattg ctaaaatacc aatcaaagcc
    13681 aataatacat gtctcgtttt cccgtgtctg ccacgtccta gtctactttt attcatattt
    13741 atatcataac aaatttgagc aataatttca atgatacaat ttggcaatcc acaattcctc
    13801 tagcaagcca aagcatctta catcaactcc aacatccgta tctgtagaga ttatgtagtt
    13861 ttctatctat ggaatttcta gtagattgaa gctagaatag tagacctata cttctaaaac
    13921 attgttagaa atcgatttga ctgtcctgat cgatttgtcc tgttctcatt tcattttact
    13981 ataaaactat gaaaagctag gtcatctgag aagtaattac tatcgacaat tgcgatagcg
    14041 tatactggtg cgatttgttt gtagctctgg tgggtatcac cttcacgttg gcgaattttt
    14101 tctaggtttt gattaacctg actgcacaga taagcccata ggcgattgat gaaaaaattc
    14161 tgatgatgaa cctgaatttc gatgataact tggattccat tatctaactc cgccaagacg
    14221 tctatactag tatagaaatc ttgcgctgag tagggcatgg aaggcaagac gtgaatgtta
    14281 cttccctcca aaatcgtcac attttttgct ggcaagtcca gcatatcgcg gataaattga
    14341 caaatcatta gttgggctga tacctggatg tctgagaatc ctccttttcc tccttctatt
    14401 atagcacatt tttaaatcta ggtttgctag attctatgct tctatctatt tattcggaaa
    14461 agaaagctaa tagcttattt gaaattttct cctctctgtt ggatagtccc gtctatctat
    14521 gttataatga aagaaggatt taaaatcgga aaaggagtat ttatgctaaa attaggtgtc
    14581 atcggaactg gcgctatcag ccatcatttc atagaagcag cccataccag tggagaatac
    14641 caactggtcg caatctattc tagaaaacta gaaactgcag caacctttgc ttctcgctat
    14701 cagaatatcc aactattcga tcaattagaa gtcttcttca aatcttcttt tgatttagtc
    14761 tatatcgcca gtccaaactc cttacatttt gctcaggcaa aagctgcctt gtctgctggt
    14821 aaacatgtta ttctcgaaaa gccagctgtc actcaaccac aagaatggtt tgatttgatt
    14881 caaacagctg aaaaaaataa ctgttttatc tttgaagcag ctcgtaatta ccacgagaaa
    14941 gcctttacta ctatcaaaaa ctttttagca gataagcaag tgttaggagc agatttcaac
    15001 tatgccaaat actcttccaa gatgcctgac ttgttggctg gacaaacacc aaatgtcttt
    15061 tcagaccgtt ttgctggtgg agccctcatg gatttgggga tttatcccct ctatgctgct
    15121 gttcgtcttt ttgaaaaagc taatgacgcg acttaccatg ctcaacagct tgacaatagc
    15181 attgacctaa atggagatgg tatcctcttc tacccagact atcaagttca catcaaggct
    15241 ggaaaaaaca tcacttccaa tcttccttgc gagatttata caacagatgg aaccttgact
    15301 ctcaacacga ttgagcatat tcgctcagct atttttaccg accaccaagg aaatcaagtc
    15361 cagcttccta tccaacaggc tcctcatacg atgactgagg aagtcgctgc atttgcacac
    15421 atgatccagc aaccagatct gaatctctac caaacttggc tgtatgatgc aggctctgtt
    15481 catgagctac tatataccat gcgccagact gctggcatta gatttgaggc agaaaaatga
    15541 aaaccaaact accgactgaa tggcaagaac tgagtgacca actcggtttc caagaattca
    15601 cccccattca aactcaacta tttgagcccc ttcttgctgg agaaaacctc ctaggagtga
    15661 gccaaaccgg aactggtaag accctagctt atctcctacc aagtcttctc agactacaaa
    15721 agaaaaaagc ccaacaactc ttgattctag caccaaatac ggaacttgct ggacagattt
    15781 ttgatgtatg taaaacgtgg gcagaagcta tcggattgac tgctcaactc ttcctttcag
    15841 gttcaagtca gaaacgacaa attgaacgtc ttaaaaaggg gccagaaatt ctgattggaa
    15901 ctcctggccg tatctttgaa ctcattaaac ttaaaaaaat caagatgatg aatgtggaaa
    15961 ccatcatcct ggatgaattt gaccaattac tagatgattc tcagattcac tttgtagaaa
    16021 aaatcactca ctacgcacct cgtgaccacc aactggtcta catgagtgcc acgaccaagt
    16081 ttgatcaaga aaagattgta ccgaatacac gcactattga tctctctgat caaaaattgg
    16141 acaatatcca gcatttctac atgcaggtag accaacgtca tcgagtggat atactacgaa
    16201 aactggctca tgtagaggat ttccgtggtc tagtcttctt caatagcctg tcagaccttg
    16261 ggaatgccga ggaaaaacta cagtatcgtg atatattggc tgtttccctc gctagtgatg
    16321 ttaatgttaa atttagaaaa gtcatcttag aaaagtttaa agataaccaa ctaaccctgc
    16381 ttcttgcaac tgaccttttg gctcgtggaa ttgatatcga tagcctagaa tgtgtcgtaa
    16441 actttgatat tcctagagat agcgaaacct acacacaccg tgctggccgt actggccgta
    16501 tgggcaagga aggatatgtt attactctcg tcactcatcc agaagaaatc aaaaaactta
    16561 aaaagtttgc aagtatccga gaaattgtcc taaaaaacca agaactctat atcaaataaa
    16621 gccccattgc tctatacaca tccagtgtat ggagcttttt tacaagcaat tcaattttat
    16681 actcatctgt tttcaaaaag cattctaatc catctccgat taacgatgga ctttatcacc
    16741 tccttctcca gttcttgtat aatatcttga agttgattca tgacatcttc caaagttcga
    16801 aaggctttat tcttaaatcc acgtttacga atctctttcc acacttgttc aacggggttt
    16861 atctctggtg tgtatggagg aataaatgca aagccaatat tagtcggaat ttttaaggta
    16921 cttgatttat gccatatagc attgtccata acgagtaaaa gataatcatc tggataagct
    16981 tgtgaaagct cttctaaaaa ggcgttcatc cactcagtat tacatccacc agctcttaag
    17041 aaaaatgatt cgcctgtatg ggcatcaaca gctccataac aatagcgaaa ttctcgtata
    17101 tagtgactat ggacatgtgg acctactcct attggagacc aacaagatcc cagtttactg
    17161 attctaccga aaccagcctc atcttggtac atcgagcgaa ccttatgaaa acgtctactg
    17221 gttttaaagc gctttctttt cttcttgaat tgagatttta tttttagacg cgacaatggt
    17281 ttgagcgtct gctttcttag gatgttctgg acgtggcata atatttcgcc aaccatggcg
    17341 cttcaacagt tgatagaagg catcacgtgt gtaggaacga tataagcctg aaataagg
//
LOCUS       DAVLRT010000038        16783 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLRT010000038 DAVLRT010000000
VERSION     DAVLRT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16783
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.038"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            167..781
                     /locus_tag="VXM85_001259"
     CDS             167..781
                     /locus_tag="VXM85_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1345835.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGNAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFALILSSVVTGKVG
                     SLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAVLRLVF"
     gene            885..1715
                     /locus_tag="VXM85_001260"
     CDS             885..1715
                     /locus_tag="VXM85_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1345836.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKIFDKITVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            1756..1833
                     /locus_tag="VXM85_001261"
                     /pseudo
     CDS             1756..1833
                     /locus_tag="VXM85_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            1937..2767
                     /locus_tag="VXM85_001262"
     CDS             1937..2767
                     /locus_tag="VXM85_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1345837.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2921..3775
                     /locus_tag="VXM85_001263"
     CDS             2921..3775
                     /locus_tag="VXM85_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1345838.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
     gene            3804..5177
                     /locus_tag="VXM85_001264"
     CDS             3804..5177
                     /locus_tag="VXM85_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1345839.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAYFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQRHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEDRMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            5170..6231
                     /locus_tag="VXM85_001265"
     CDS             5170..6231
                     /locus_tag="VXM85_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1345840.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEESSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            6233..6925
                     /locus_tag="VXM85_001266"
     CDS             6233..6925
                     /locus_tag="VXM85_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345841.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIVIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6955..7524)
                     /locus_tag="VXM85_001267"
     CDS             complement(6955..7524)
                     /locus_tag="VXM85_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1345842.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7659..8273
                     /locus_tag="VXM85_001268"
     CDS             7659..8273
                     /locus_tag="VXM85_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1345843.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFISSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            8275..9921
                     /locus_tag="VXM85_001269"
     CDS             8275..9921
                     /locus_tag="VXM85_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1345844.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKVSPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIINVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9933..11219
                     /locus_tag="VXM85_001270"
     CDS             9933..11219
                     /locus_tag="VXM85_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1345845.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            11280..11813
                     /locus_tag="VXM85_001271"
     CDS             11280..11813
                     /locus_tag="VXM85_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1345846.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11891..12361
                     /gene="nrdI"
                     /locus_tag="VXM85_001272"
     CDS             11891..12361
                     /gene="nrdI"
                     /locus_tag="VXM85_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1345847.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12654..13916)
                     /locus_tag="VXM85_001273"
     CDS             complement(12654..13916)
                     /locus_tag="VXM85_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1345848.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIIIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(14226..14684)
                     /locus_tag="VXM85_001274"
     CDS             complement(14226..14684)
                     /locus_tag="VXM85_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1345849.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(14681..15121)
                     /locus_tag="VXM85_001275"
     CDS             complement(14681..15121)
                     /locus_tag="VXM85_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1345850.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            15554..16420
                     /locus_tag="VXM85_001276"
     CDS             15554..16420
                     /locus_tag="VXM85_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1345851.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
BASE COUNT         5106 a         3025 c         3772 g         4880 t
ORIGIN      
        1 gtgttttgag ctgacttcat cagttctatc tgcaacctca aaacagtgtt ttgagcaacc
       61 tgcggctagt ttcctagttt gctctttgat tttcattgag tattattaag acaatatgag
      121 gagaaagatg aaagaaaaag gattttagga gggggcgcag gcagccatgc caacggctct
      181 tggctatgtc agtatcggcc tggcctgtgg gactatcggt gcgccctatg tgacacctgt
      241 tgagatgggc ttgatgagtc tctttgttta tgctgggaat gcccagtttg ccatgttggc
      301 actgattgtg gttcaagctc ctgtggcagc tattgctatg acggtttttc taatcaactt
      361 gcgtctcttt ttgttgagtt tacacgcatc gacttatttc cgtcatacca gtctttggta
      421 caatatcggt atgtctagta tcttgacaga tgagacctat ggcgttttga tgggtgaatt
      481 ggcccataca gacaaggtaa atcctatgtg gatgcacgga aacaatctta acagctatgt
      541 ggcttggttt gtggggacag tagtcggaac ggctctgggt ggcctgctac caaatccaga
      601 aatctttgcc ttgattcttt caagcgtagt gacaggtaag gttgggagcc ttcctcaaat
      661 taaatggcta gacttcttag ccgtctttcc aacagcttgg gtagcctttc gctaccgcaa
      721 tctagtcgga acagttctct ttggagtagt cttgattgcc gtcttgcgtt tggtctttta
      781 aatcagccat aaagaaaacc tatcacagat atatatatca tataatggtg taaatgattt
      841 ttttctgtta agactataag gtattctatt ttggaggaaa tgacatgaaa aaaatcgtta
      901 aatactcatc tcttgctgcc ctagggcttg ttgctgcagg tgtgctagca gcttgctcag
      961 gtggtgctaa gaaagaagga caagcagcta gcaagaaaga aattattgtt gcaactaatt
     1021 cttcaccaaa accattcaac tatgaagaaa atggcgaatt gactggttac gagattgaag
     1081 tcgttcgcgc tatctttaaa gactctgaca agtatgatgt caagtttgag aagacagagt
     1141 ggtcaggtgt ctttgcgggt cttgacgctg accgttacca aatggctgtt aacaacatca
     1201 gctacactaa agaacgtgcg gagaaatacc tttatgcagc cccaactgtt aaagacccta
     1261 acgttcttgt agtcaagaaa gacgattcta gcatcaaatc gcttgatgat gttggtggaa
     1321 aatcaacaga agttgttcaa ggaacaacat ctgctaaaca gttagaagac tacaacaaac
     1381 aacactcaga taatccaact atccttaact atactaaagc aaacttacaa caaatcatgg
     1441 gccacttgag cgatggtcag tttgactaca agatttttga taaaatcact gttgaaacag
     1501 tcatcaagaa ccaaggtttg gacaacttga aagtcatcga acttccaagc gaccaacaac
     1561 cttacgttta cccacttctt gctaaaggtc aagatgagtt gaaaacattt gtagacaaac
     1621 gtatccaaga actctacaaa gacggaactc ttgaaaaatt gtctaaacaa ttcttcggag
     1681 acacttacct accggcagaa gctgatatta aataatttct tgaaatcatc cattctgagt
     1741 aagatgggtg atttttcagt tcgctaccgc aatctagtcg gaacagttct ctttggagtg
     1801 gtcttgattg ccatcttgcg tttggtcttt taaattaccc ataaaaaaaa cttatcacag
     1861 agatagatat catataatgg cgtaaatgct ctttttctgt taagattata aggtattcta
     1921 ttttggagga aatgacatga aaaaaatcgt taaatactca tctcttgctg ccctaggact
     1981 tgttgctgca ggtgtgcttg cggcttgctc agggggtgct aagaaagaag gagaagcagc
     2041 tagcaagaaa gaaatcatcg ttgcaaccaa tggatcacca aggccattta tctatgaaga
     2101 aaatggcgaa ttgactggtt acgagattga agtcgttcgc gctatcttta aagattctga
     2161 caaatatgat gtcaagtttg aaaagacaga atggtcaggt gtctttgctg gtcttgacgc
     2221 tgaccgttac aatatggctg tcaacaatct tagctacact aaagaacgtg cggagaaata
     2281 cctctatgcc gcaccaattg cccaaaatcc taatgtcctt gtcgtgaaga aagatgactc
     2341 tagtatcaag tctctcgatg atatcggtgg aaaatcgacg gaagtcgttc aagccactac
     2401 atcagctaag cagttagaag catacaatgc tgaacacacg gacaacccaa ctatccttaa
     2461 ctatactaag gcagacttcc aacaaatcat ggtacgtttg agcgatggac aatttgacta
     2521 taagattttt gataaaatcg gtgttgaaac agtgatcaag aaccaaggtt tggacaactt
     2581 gaaagttatc gaacttccaa gcgaccaaca accgtacgtt tacccacttc ttgctcaagg
     2641 tcaagatgag ttgaaatcat ttgtagacaa acgcatcaaa gaactttata aagatggaac
     2701 tcttgaaaaa ttgtctaaac aattcttcgg agacacttat ctaccggcag aagctgatat
     2761 taaataattt cttgaaatca tccattctga gtaaggtggg tggtttctca gtttttaggg
     2821 atatagtttt aaactatata tctgactatc taataacaat ttgtgaaatc aaacaaattg
     2881 tgagatacta gtacggtatt atttttaagg agaaagaatc atgaaaatca aaaaatggct
     2941 tggcctagca gcccttgcta cagtcgcagg tttggctctt gcagcttgcg gaaactcaga
     3001 aaagaaagca gacaatgcaa caactatcaa aatcgcaact gttaaccgta gcggttctga
     3061 agaaaaacgt tgggacaaaa tccaagaatt ggttaaaaaa gacgggatta ccttggaatt
     3121 tacagagttc acagactatt cacaaccaaa caaagcaact gctgatgggg aagtagattt
     3181 gaacgctttc caacactata acttcttgaa caactggaac aaagaaaacg gaaaagacct
     3241 tgtagcgatt ggagatactt acatctctcc aatccgcctt tactcaggtt tgaatggaag
     3301 tgccaacaag tacactaaag tagaagacat cccagcaaac ggagaaatcg ctgtaccgaa
     3361 tgacgctaca aacgaaagcc gtgcgcttta cttgcttcaa tcagctggct tgattaaatt
     3421 ggatgtttct ggaactgctc ttgcaacagt tgccaacatc aaagaaaatc caaagaactt
     3481 gaaaatcact gaattggacg ctagccaaac agctcgttca ttgtcatcag ttgacgctgc
     3541 cgttgtaaac aataccttcg ttacagaagc aaaattggac tacaagaaag cacttttcaa
     3601 agaacaagct gatgaaaact caaaacaatg gtacaacatc attgttgcga aaaaagattg
     3661 ggaaacatca cctaaggctg atgctatcaa gaaagtaatc gcagcttacc acacagatga
     3721 cgtgaaaaaa gttatcgaag aaacatcaga cggtttggat caaccagttt ggtaataaga
     3781 aacagggagg tgggagagag aatatggttt ttcctagcga acaagaacag attgaaaaat
     3841 ttgaaaagga tcatgtagcc cagcattatt ttgaggtttt gcgtaccttg atttctaaga
     3901 aatcagtctt tgcccagcag gttggactca aggaagtcgc aaattatctg ggtgagattt
     3961 tcaagcgtgt tggagctgaa gtggagattg atgagagcta tacagcgccc tttgtcatgg
     4021 catatttcaa gagttcgcgt ccagatgcca agaccttgat tttctataac cactatgaca
     4081 ctgtgccagc ggatggggat caggtctgga cagaggatcc ttttacgctt tcggtccgca
     4141 atggcttcat gtatgggcgt ggggttgatg acgacaaggg tcatatcaca gctcgcttga
     4201 gtgctttgag aaaatatatg cagcgccatg atgatttacc tgtcaatatc agctttatca
     4261 tggagggagc ggaggaatcg gcttcaacag acctagataa gtatttggaa aagcatgcag
     4321 acaaactccg tggggcggat ttgttggtct gggaacaagg gaccaaaaat gccttggaac
     4381 aactggaaat ttctggtggc aataagggga ttgtgacctt tgatgccaag gtaaaaagcg
     4441 ctgatgtgga tatccactcg agttatggtg gcgttgtgga atcagctcct tggtatctcc
     4501 tccaagcctt acagtctctt cgtgctgcgg atggccgtat cttggttgaa ggcttgtacg
     4561 aagaagtaca agagcccaat gaacgagaaa tggccttgct agaaacttat ggtcaacgaa
     4621 acccagagga agttagtcgg atttatggat tggagttgcc tctcttacag gaggatcgga
     4681 tggcctttct aaaacgtttc tttttcgagc cagcgcttaa tatcgaagga atccagtctg
     4741 gttatcaagg tcagggtgtt aagactattt tacctgcaga agccagtgcc aagctagagg
     4801 ttcgtctggt tccgggccta gaaccgcatg atgttctgga aaaaattcgg aaacagctag
     4861 acaaaaatgg ctttgataag gtagaattat actatacctt gggagagatg agctatcgaa
     4921 gcgatatgag cgcaccagcc attctcaatg tgatcgagtt ggccaagaaa ttctatccac
     4981 agggcgtttc agtcttgccg acgacagcgg ggacaggacc tatgcatacg gtctttgatg
     5041 ccctagaggt accaatggtt gcattcggtc taggaaatgc caatagccga gaccacggtg
     5101 gagatgaaaa tgtgcgaatc gctgattatt acacccatat cgaattagta gaggagctga
     5161 ttagaagcta tgagtagaga tattatcaag ttagatcaga tcgatgtgac ttttcatcaa
     5221 aagaagagaa ccattacagc ggttaaggat gtgaccattc acatccaaga aggggatatc
     5281 tacggaatcg ttggatattc tggagcaggg aaatcaaccc ttgtacgggt gattaacctc
     5341 ttgcaaaaac catctgcagg gaaaattacc attgacgacg atgtgatttt tgatggtaag
     5401 gtcaccttga cggcagagca gttgcgtcgt aaacgtcaag atatcgggat gattttccag
     5461 cattttaacc tgatgagcca aaagacagca gaggagaatg tagcctttgc ccttaaacac
     5521 tctggactca gcaaggaaga aaagaaggct aaagtagcta agttgttgga cttggttggt
     5581 ttggcagatc gtgctgaaaa ctacccttca caactatctg gagggcaaaa acagcgtgtg
     5641 gcaattgcgc gtgccttggc caatgatcca aaaatcttga tttcagacga gtcaacttct
     5701 gcccttgacc ctaagacaac caagcagatt ttggccttgt tgcaagattt gaaccaaaaa
     5761 ttaggcttga ctgttgtcct gattacgcat gaaatgcaga ttgtcaaaga cattgccaac
     5821 cgtgttgcag ttatgcagga tgggcatttg attgaagaga gtagtgtgct tgaaatcttc
     5881 tcagacccta aacaaccttt gactcaagac tttatctcaa cagctacagg tattgacgaa
     5941 gccatggtca aaatcgagaa gcaagaaatc gtggaacact tgtctgaaaa cagtctcttg
     6001 gtgcaactca agtacgctgg atcttcaaca gacgagccac ttttgaatga attgtacaag
     6061 cattatcaag taatggctaa tattctctat gggaatatcg aaatcctcga tggtactcct
     6121 gttggagaat tggtggtggt cttgtcaggt gaaaaagcag cgctggcagg tgctcaagaa
     6181 gccattcgtc aagcaggcgt acagttaaaa gtattgaagg gaggacagta agatggaatc
     6241 attgattcaa acctatttac caaatgtcta taagatgggt tggtctggtc aggcaggctg
     6301 gggaacagct atctacctaa ccctctatat gacagttctt tccttcatta tcggaggctt
     6361 cttggggcta gtggcaggtc tctttctcgt cttgacagcg ccaggtggtg tcttggagaa
     6421 taaagtcgta ttctggattt tagacaaaat tacctcaatt tttcgtgcgg ttccctttat
     6481 catcctcttg gcaatcttgt caccactttc tcacttgatt gttaaaacaa gtatcgggcc
     6541 aaatgcagcc cttgtcccac tttcttttgc agtctttgcc ttctttgccc gtcaggtgca
     6601 ggttgtcttg gctgaattgg atggcggtgt cattgaggcg gctcaagcga gcggagcgac
     6661 tttctgggac atcgtgggtg tttacctatc agaaggtctt ccagatttga tccgtgtgac
     6721 gactgtgacc ttgatttccc ttgttgggga aacagctatg gccggtgcgg ttggagctgg
     6781 tggtatcggt aacgtagcca tcgcttatgg atttaaccgc tacaatcacg atgtgaccat
     6841 cttggcaacc atcgttatca ttttgattat ctttgcaatc caattcttag gagatttctt
     6901 gactaagaaa ttgagccata aataaaaaag agccgtgtgg ctctttttaa ctgatcagat
     6961 tttctgggca aattttttac tcaaggcttg tccaatcaag gcacccacta gggctccgat
     7021 gacaatactt gcgataaata gaaggacagt tccagggttt ggagcgacca tgatgcggtc
     7081 gatatattct tgggattttc ctcttgccag aagagtagcc atataggctt tgggcgcaat
     7141 ccacataagc aagattggtc ctgttgtact aaaggcgaaa ataatgaaag aaaggaagtt
     7201 ctttgttttg tccttgtatt ttcctaaatg agctactcca tctgctagga ggccacagat
     7261 aattccagga aggaaggaac cagcaccgtg tttagttccc aagaaaaaga gggcaatgac
     7321 aaggccgata gtggtaatgg ctccaaagcg cggaactttt gcgactagga tcatatagac
     7381 gctaccgccg acaagggcag taaaggcagg cgcataaaac atgtttccag tttggtcaaa
     7441 gagattgccc aaaaggacac caatccccat gcagaggaag taaagaactg caaaaagcag
     7501 tgtagttaag atagatttct tcatgaattg tctcctgata attttttcac aattctcatt
     7561 ttaccatggt ttggtgggat tgtaaatgga tactagaagt ttttgaaaac gcttgaaata
     7621 tgatataatg gtttcatagt tatttttagg ggaatatcat gggtagattt ttagactttg
     7681 tctttaatcg tttcttttta gggatgattg cgacagcctt cttttggcta ttaactttag
     7741 caggagggat tatccttggt ctagcgccgg ctagtgccac cttgatgagc ttatatgcag
     7801 aacatggtta tagctttcgg gaatacagtt tgaaggaggc ttggtctctt tacaagcaaa
     7861 attttatctc aagcaacctg attttctata gctttttagg tgtgggtcta gttttgacct
     7921 atggtttgta tctcttggtg caattgcctc atcagaccat tgttcatttg attgcgaccc
     7981 ttttgaatgt cctagtagtt gccctgatct ttttggctta tacagtatct ttaaaattac
     8041 aagtttattt tgccttgtcc tatcgaaata gtctcaaatt atccttgatt ggcatcttta
     8101 tgagtctagc agctgtggct aaggttctcc ttgggactgt gctacttgta gcaattggtt
     8161 actatatgcc tgccctgcta ttttttgtag gaattgggat gtggcatttc tttatcagtg
     8221 atatgttgga acctgtctat gaaatcatcc atgaaaaatt ggcgacaaaa tagaatgaag
     8281 cacttttggc tacatacgct tctaagaacc tatagttcag tgatgatcat tatcattgcg
     8341 agttttgcaa tcttactctc ttacgctgac tgggattcac gtgaaaagga agcccagaga
     8401 gtagcccagc gtgtaactgc tagaacagtg agtgaaattg aatattacca tagagagtca
     8461 acccagatag ctcaggcttt agttgaaaac caagctcgta ttgagggaat ctataaatac
     8521 tttagcctta gcatgccaga ctatttttac tggcaattag agcggaaagt ttcgccttat
     8581 atatcagtct ctctgtatga aaatgttgat gacctctatg ttcgaaatga ttttgtaact
     8641 ggggtggcca ttgcttttca agattacaag gaagtctatg tttctactaa agacaaacgt
     8701 agtggagaaa aaatcagggc tgaggatttc aaaccagcag gaaatagttt tgccattcca
     8761 gtgtcagatc cagtgtcaga tcaagactta ggagtgattt acatctcctt ggatcctgct
     8821 gttttatacc atgccattga taatactaga ggtcatactc cgatggcagt aacagtgacc
     8881 tcaccttttg atacggagat ttttcatatt ggtgagacag ttgataagga gagtgaaaat
     8941 tggctagttg gcttaacttc tcatggttat caggttcagg tggcagttcc caaaaacttt
     9001 gttttacaag gaacggtgac cagctctgct ttgattgtgg gcttgagcct tctctttatt
     9061 gtcattcttt atctgacttt gaggcagacc tttgctaatt atcaaaagca ggtagtggat
     9121 ttggtggatt ccatccaagc tattgcccaa ggacaagaag gtcttcgcat tgatacgctt
     9181 gaaaaggatc aggaattgct cctaatcgcg gagacgacca atgatatgtt ggatcgattg
     9241 gaaaagaata tccatgatat ttaccagtta gagcttagtc aaaaagatgc caatatgcga
     9301 gccttgcagg cgcaaatcaa tcctcatttt atgtataata cgctggagtt cttgcgcatg
     9361 tatgcagtta tgcagagtca agatgagttg gcagatatca tttatgaatt cagtagtctc
     9421 ttgcgtaaca atatttccga cgaaagagag accctcctca aacaggaatt agaattttgc
     9481 cgtaaataca gctatctctg catggttcgc tatcccaagt ccattgccta tggtttcaag
     9541 atagatccag agttagagaa tatgaagatt cccaagttta ccttgcaacc gctggtagaa
     9601 aactatttcg cgcatggtgt tgaccacagg cggacagata atgtgattag catcaaggct
     9661 cttaaacagg atggttttgt ggaaattttg gtggtcgata atggtagagg aatgtcggct
     9721 gaaaagttgg caaatatccg agaaaaatta agtcagaaat attttgaaca ccaagccagc
     9781 tacagtgatc aaaggcagtc tatcggaatt atcaatgtac acgagcgttt tgtgctctat
     9841 tttggagacc gctatgccat tacgatagag tctgcagagc aagccggtgt tcagtatcgt
     9901 attacaattc aagatgagta gaaagggaga aaatgtataa agtattatta gtagatgatg
     9961 agtacatggt gacagaaggt ctgaagcgtt tgattccctt tgataagtgg gatatggagg
    10021 tcgtcgcaac agccagtcat gccgatgaag ctctagaata tgttcaggaa aatcctgtcg
    10081 atgtcatcat ttccgatgtc aatatgccag acaaaacagg gcttgatatg attcgggaga
    10141 tgaaagagat cttaccagat gctgcctata tcctgctctc aggttatcag gagtttgatt
    10201 atgtaaaaag agcaatgaac cttagtgtgg tggactattt ggtcaagcct gttgataagg
    10261 tagagctggg aaatctgctg gagaagattg caggtcagct cggcgagaga gggaagaaaa
    10321 gtcagactct tagtcaagaa ttagacgagg ctggatttgt tagttattta ggggataagg
    10381 agaattggtg gataggtcta tccaaggaaa aacaaggttc cttcaccatt ccctactatg
    10441 tcttgggcca agactggcag attttcattt ctgaccaacc cctagatggt ttagtcgtta
    10501 caccttttga agctccttat caagaacact ttgaacgctg gaagctgaat gctgagaaaa
    10561 ccctctttta cggttctgta aatttgcagc agtctgagag tctctttgcc tattacgaac
    10621 cgatttatag ggttatcatt cagggaaatc tcaatcaaat cgtagaagag ttaaatctct
    10681 tggagaaggt agttcttgaa aatacgccgc gtgttccgat tactaaacag ctttttattc
    10741 agtttgtcat ggatgttttc catttatttg aacatctcaa agctgatgac ctgacagaca
    10801 tcgtcaaaac cattcatgct attcaatcct tcgatgaatt ggttccttat atcaaggaaa
    10861 ctctgagcag ctttttcggt caataccgta tgaatgaaaa tgtggtcagt gtgctggaag
    10921 tcattggtcg tgattaccaa aaagagcttt ccctcaagga tatcagtaag gctctcttta
    10981 tcaatcctgt ctatctaggg cagttgatta agcgtgaaac cgattcgact tttgcagagt
    11041 tactaaacaa acaacgtatt aaggctgccc aacaactttt gctttcaact agtgacagca
    11101 tcgaagatat ttgttatgct gttggttaca gtaaccttgg atatttctat aaagttttcc
    11161 gaaaattgtg cggaaaatcg ccaaaagcct accgaaaaca ggtagaaact atactataag
    11221 atttgtattc ctttacaaaa tgtgctataa tataaatatt attattagga ggttctatca
    11281 tgaaaaagaa accaatttat ctatgggtct tgctaatctt gtctgccctt atttcagttc
    11341 cgtctctgtt tggaattgtg agtcccttgc ctagtaaaga agcccttcgt gctgctcaga
    11401 aacaagttgc aggggtcaat gctcagcaat tagaagacca gcttaattat acctatagag
    11461 tagcagaagc atctcattct atttttaatg ttgctttgat tgtgctatct actattttag
    11521 tggtcgtagc gattgtcttc cttgttcgta aaaatttgca atatgcaaac tatacttacg
    11581 ttggctatgt tttgttagct attattggtt cgatttatgg ctatgttggt ttacaagacg
    11641 ctgtgcagtt ggttcaagat gagagcatgc gtttgacagt gagtattggt tcaaaagctg
    11701 ttagcatttt ctatatcgtc atcaatgttc tcttcctagc ccttgtcttc tataagatgt
    11761 ggcgacaaca gaaagccttg gcagaagaag aggaaacaga agagcttgcc taagtattga
    11821 caaaaaagaa ctatcaagat acaatcttgg tagttgtttt ttgattatag ataaaatcat
    11881 ggaggtacat atgaagacaa tttctttagt ttatattagt ttgagtggca acactgaaag
    11941 ttttgtgacg cgcttgaaag actatctctt gtcccagtac aaagggattg aggttcaaaa
    12001 gattcatatc aaggatttgg tcaaggaagg caaaaatttc tacgaaatgg accatcccta
    12061 tgttgctttt ttgccgacct atctcgaagg tgggaatggc gtggataacg gagatgttga
    12121 gattttgacg acaccggtgg gagattttat tgcctatggt aacaatgcta gtaagtgttt
    12181 tggtgtggtt gggtcaggaa atcgtaactt taataaccaa tactgcctaa cagccaagca
    12241 atacagtcaa cgctttggct ttcctgtatt ggctgacttt gaaatgcgag gcatgctgga
    12301 agatatcaaa catgtcgcag ctattatcgc agatttgtat gagttggaaa aggagaatta
    12361 atactcaatg aaaatcaaag agcaaactag gaagctagtc gcaggttgct caaagcacag
    12421 ctttgaggtt gcagatagag ctgacgtggt ttgaagagat tctcgaatag tataaagttc
    12481 ttataagagg tggttgaagt tgttcaactg ttttttgagt ataaacagtc tttaagaacg
    12541 taaaaaagca taagttcagg tcttcaaaac cttgttctta tgctttttat catagaaata
    12601 tttgctgaat tttctcttga aatgaaacct tatcttcatc tcgcgcttgt cccttaccct
    12661 gcttttccga ttgcgagtgc gtagataaag aagatggcga agccaaagag gaaaatcaat
    12721 cccgcaatgg caaggtgttt gagccaagaa ggaagatttt tgttttcacg tgttaccaag
    12781 gcgtaattca aaagagcaaa gaaaggtgtt gtcaggaaag agccaatcat ggcaaagcgg
    12841 agcatggttg aaacctgacc agcgaagaac ttgataatga tgataccgat gatagcagtg
    12901 atggtcatcc agatgttcaa agatttacga ttatcctctt tttgactgat tagcagtcgg
    12961 agagattcct gattaacgcg agaatagcca tcgataacag ttataactgt tccaaagata
    13021 cagaggaagg caataaaggt aatcaagtaa cgggaccatt cgccaagaac agaggcatac
    13081 atgcccacga attgagagat gtatttggct gaagcagctt caaccgcctg ccctgtagga
    13141 tactgaatca gtgctcccag tgccacaaag aagacggcta ggatagctgt tccaatataa
    13201 ccagtgttaa agtcaaacag agcgtcttct gtgttaaagt tgacggtctt tctcttttca
    13261 gctgaccaaa gtgaattgat ggctgaaatt tcaataggag ccggcatcca tcctaggagg
    13321 gagacgatga agggcagagc tgccatttgc caaggtgtct tctcgacaaa atcagaactg
    13381 tattctggat gcttgaccgc cgcaatgata actgcaagaa cagtcgcaat ggttaaggca
    13441 gacataatcc atttgaccat gccgtctaaa agtttgtagc ctccaaagag tagcatagcc
    13501 caaatgattg caacgagaat gagggaccac tgagtaatgc taagtccaat cattgggaag
    13561 gcactggcga tgatagctga gcacagaatg gcaacaccag ccgtgttgac catagccgaa
    13621 aagacattga ggataaagaa aatccagaga tagagttttc ctttttcggc ataaccttca
    13681 accaaagtct ttccagtatc agctgtgtat tcagcaccaa aacggaaaaa tggatattta
    13741 aagacattgg ctaagatgac caagagaagt agagaccaac cgtaagaacc gccagcttga
    13801 gttgaggata caatgtggga acctccaaca gcggcagttg ccattaggat tccaggtccc
    13861 attgctttta gcttgcttgc ccaggttgat tgatttaagg aaatagcttg cgacatgata
    13921 aatactcctt tttgaatttt cagaataatc tgtatatgta atctatattc tacaaaaaat
    13981 tttggaaaaa tgcaagagta ttttgaaaaa agatagaaaa tttacaaaga aaatctgaaa
    14041 taactgttat gatatagagg attgaaacta gaatagtaca cctctgcttc taaaacattg
    14101 ttagaaatcg atttgactgt cctgatcgat ttatcctgtt cttatttcat ttcactatag
    14161 taaggtgcag caggataaag aaaatccgag aagtttcttt ctcggatttg cttattatga
    14221 ggttcctatt ttctatgctc caagagttga ttgatgtgat ctactttttt agcctctctt
    14281 ttatagagaa tagcccagat gatgagatag acaatcgcaa actcgataat gagctggagg
    14341 tagaaaatcc agtggaaagg gaaccaacca gcaagagttg ctagtgggac aaagccagcc
    14401 agcataagga agaaatgagt cagagtggca cgaagcatgc tccagtcacg gctaaataag
    14461 cggttgccaa agttgaagag cataccgatg gttgcccata taagtgtgca gtagagcaag
    14521 accagggcac cgtgaacctg atgctgatcc atcacttggc cgatgagaga gtagggattt
    14581 agtggtgcgt aggtatttgg tgcataaatg agtgaaaaga ggatagagag gatgaggccg
    14641 ataagaacac cggtagctgc atcgtgaaat acttgttttt tcatagttct aatttctcct
    14701 tgatggtttt tagataacgg cgtgaagagt aggtgaagct ttcgtttttc aagaaaattt
    14761 ctaccagacc gtttggcgtg agcttgaggt gagagatgga atcgatattg atgatttctg
    14821 attgggaaat ttggataaaa ttggttggca agagtttaag aacctgatag agtcgcaaat
    14881 caatgctgta ggtctgactc gcggtttctg ctagaacctt ccgattctcg atatagaagc
    14941 gctgaatctt gccaatctca actagataga cctgatcatc gatttttcct ttgatttttt
    15001 ctctttggtc cagattttct gcgaactcga tgactttctg gactttttcg gtttcttgag
    15061 gtgcttggac aatcagcttt tcctcctcgt aagtctcact aatctgtagt tctactttca
    15121 tagttttctc tccttttcag ttatacaagg ttgtgatcac ttcctgtata tccttattaa
    15181 acactatttt atagcccatg gcaaggaacc ttgtctatgt ggagggattg ggctcctatg
    15241 tggtggagct tttctgttct ttctgaaata tggtataata gcactaatca atttctagga
    15301 aaatagatac ggaaaggggc tgaaagaact aggggaacag ttggaattag aaagtgatag
    15361 gtatgaaaag tggcgttgaa gctgaataag tttataaagc gtttgaatga agaaatggaa
    15421 agtaaataag ttgacgatga attacaaaat agattttatc tacaatttgt atctttcaaa
    15481 tcattgattt gaattttaga tttaatcatg ttacaattaa aactgaaatc aaatagaaga
    15541 ggaaaatgca aacgtgggca cactattagc aacaagatta aaaaatagac ggaaagaatt
    15601 aaaaatgtct cagcgagaac tagctgaagg tatctgtaaa caggggcaaa tcagtcgatt
    15661 agagagcgga gagtttaccc ctggagcgga tttcctgaat gcccttgcta agaagttaaa
    15721 agtcagtatg gattattttt ttgatgagca ggttgtagag aaagttgagg agttatcaga
    15781 atttaagaaa ttagcacaga catttattac aaatcgcaat tatgagtctt tgaaatatat
    15841 atatgaattg gagaatgtaa aagctcaccg cctatcttta gcagataaaa tctacatgga
    15901 atggataaaa tctctgatag atttttattt ttacggacag aaagaagatg cgattgtacg
    15961 attagagggg gtattatcac agttaagtgt atctgatttg aactatctcc aagtttcaaa
    16021 caccctattc aatttttatt atgatattgg agatttaggt cgtttcaata aaattcgtga
    16081 aaaactagag tatcaagtaa atcacctcaa tttgagtaca atcgaagagt tagaactatc
    16141 tattaagttt aactacaacg tttgtcgtta tctatggtta cagaaaaata ttgaagaagc
    16201 tattactaaa attacagcta ctataaaaca atgtaaggct tacagaacga attatctttt
    16261 ggctgatttg tacttattga tgggaaatgt aagtgaaaat ttttcttcta aaagttcggt
    16321 gaaagaatat tttgagacgg catattttct atacaaactt gaggagaaca tgtcaatgac
    16381 tctgaaagtt gagcattaca ttgcagatat aacagaataa tgaatacggt gttcaagtga
    16441 gcatcgtttt tcaaacctag ttcagataca aaaaagtatc aaacaaaaaa aaataaaaaa
    16501 acattataaa aacaattgac aagctaattt tgaagtgata aattaaatgc aagaaaacaa
    16561 aaggagaggt atatgagaag tttgtttaag aaaatagttg ctgtattagt tattggctta
    16621 attctgttag gaattgcgag aacacctcaa gttcataaga tggctagagg gattgatcct
    16681 ggtcctgcga attttattta gaaatcaatt tctactaatt gagtactaat tgagtattaa
    16741 tgggtaaaaa gattgtatgg ataagaaaaa tttacttgta ttg
//
LOCUS       DAVLRT010000039        16537 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLRT010000039 DAVLRT010000000
VERSION     DAVLRT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.039"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            14..1945
                     /locus_tag="VXM85_001277"
     CDS             14..1945
                     /locus_tag="VXM85_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1345852.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            1932..3248
                     /locus_tag="VXM85_001278"
     CDS             1932..3248
                     /locus_tag="VXM85_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1345853.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            3423..4409
                     /locus_tag="VXM85_001279"
     CDS             3423..4409
                     /locus_tag="VXM85_001279"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1345854.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVSQLKADTPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4473..4544
                     /locus_tag="VXM85_001280"
     tRNA            4473..4544
                     /locus_tag="VXM85_001280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4506..4508,aa:Arg,seq:ccg)
     gene            4727..5425
                     /locus_tag="VXM85_001281"
     CDS             4727..5425
                     /locus_tag="VXM85_001281"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1345855.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5416..8955
                     /gene="smc"
                     /locus_tag="VXM85_001282"
     CDS             5416..8955
                     /gene="smc"
                     /locus_tag="VXM85_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1345856.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENACKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIVIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8952..9746
                     /locus_tag="VXM85_001283"
     CDS             8952..9746
                     /locus_tag="VXM85_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1345857.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            9746..10564
                     /locus_tag="VXM85_001284"
     CDS             9746..10564
                     /locus_tag="VXM85_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1345858.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVSEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10568..11845
                     /gene="ftsY"
                     /locus_tag="VXM85_001285"
     CDS             10568..11845
                     /gene="ftsY"
                     /locus_tag="VXM85_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1345859.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEAERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11889..13376)
                     /locus_tag="VXM85_001286"
     CDS             complement(11889..13376)
                     /locus_tag="VXM85_001286"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1345860.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(13525..14265)
                     /locus_tag="VXM85_001287"
     CDS             complement(13525..14265)
                     /locus_tag="VXM85_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1345861.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14265..16430)
                     /locus_tag="VXM85_001288"
     CDS             complement(14265..16430)
                     /locus_tag="VXM85_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1345862.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         5175 a         2938 c         3656 g         4768 t
ORIGIN      
        1 aggagttaga ttgatgaata aaagaaaagt tagtttagaa gatttttata aatggtatag
       61 tctaaataaa gaagagttat taaataaggc aactgttggt gaaaagttta atgataaatt
      121 aaaagaagag tttctccagg aatggccttt ggataggatt ttaacaatgt caatcgatga
      181 atatgtaata ggaaagggac agcaaaataa gtctttatgc tacgctcttg agaagggaaa
      241 atacaaaaat ctatttcttg gaatttctgg tggctcagct tcaaaatttg gtatttattg
      301 gaataaaaaa acaaacaaat ataaagatca agctaataat gagatttcag agttggatca
      361 gcgattttca aaattaaaat cagatttgta tgaaattatc aaagaaggta ttcgttttaa
      421 ctttgaaaat cctatttttg atatgaaaag atcaacaaat gaatttattg gtcgttctgc
      481 tatggtgaca aaattacttt gtatctatac tgagggagat cctttctttg gtgtaaatat
      541 taatagtcag aaagaatttt ggaaccactt tgtttctcag acaaatcaag gtggacctta
      601 tctgcaaaat cataaaataa ttgaactggt gtccaaaact tatcctgagt tggagccatc
      661 gaaattagga actatgcttt ttgagtattc taagcttttt atggaaaata aggaagacaa
      721 tagtacaatg gattcatcaa acaattttag tcatcaatta actcaatctc tattaaagtc
      781 tccaaacctt atccttcgtg gtgctcctgg cacaggaaag acttatcttg ctaaagaaat
      841 tgccaaagaa ttaacggatg gcaacgaaga ccaaatcgga tttgtacaat ttcacccatc
      901 ctatgattat acggattttg tagaaggttt aagaccagta tcaaatgggg atggagctat
      961 tgagtttagg ctacaggacg gtatttttaa agatttttgt cagaaagcaa aagaaaccca
     1021 attgattgga ggacaagata attttgatga ggcttgggat tcttacttag aatatataaa
     1081 tgttgctgaa gaaaaagaat atataacaaa aacatcttac ttatctgtta atagtagaca
     1141 aaatttgtca gtaaattatg atagtggtgt tccaggatgg tcactaccta gcaaatatgt
     1201 ttacgagttg tataaagata aaaattataa taagcaagaa tactacaaaa gtggtggaaa
     1261 aactgtccta gaaacattga gaaagagatt tggtttgaaa gactatgttt ccccaacaga
     1321 aattgatact gataagaaat tcgtcttcat catcgatgaa atcaatcgtg gggagatttc
     1381 taagattttt ggcgaactct ttttctctat cgaccctggc tatcgtggtg aaaaaggaag
     1441 tgtttctacc caatatgcaa atctacacga aactgatgaa aagttctata tccccgaaaa
     1501 tgtttacatc atcggaacta tgaatgatat tgatcgttca gtggatacct ttgattttgc
     1561 tatgcgtcgt cgttttcgtt ttgttgaagt tactgccgag ggtcaagttg gcatgttgga
     1621 taaagagctg aatatccatg cagaagaagc aaaaattcgt ctaagaaact tgaacgctgc
     1681 tatcgaaaat gttcaggaat taaacagtca ttatcatatt ggaccaagtt attttcttaa
     1741 gttgaaggat gtagattttg actatgaatt actctggtct gattatatta agcctctcct
     1801 agaagactac ttgcgaggtt cttatgatga ggttgaaact ttggaaactt tgaaaaaagc
     1861 atttgaactg acaaataatg agcaaaaaga tcaggcagta gctgatgaca atgaaggcga
     1921 tgaaaacgat gatgcggatt actgataatc aacacaagat tattaaagaa aaatttgttg
     1981 aagaatatcc taaactaagc aatcttcttt tagacagaac cttggaaagt ctatcccaag
     2041 atgaacgtat tttcattttt ccaaatgatt tgactcatac tcctgatttg gataaggacc
     2101 aaaagatttt tgaaacagtc aatcagaaaa tcaagacagg gaacgtgatt ggttttcttg
     2161 gatatggtca ggaaagatta acgatttcct cacgattttc tgatgagagt aatgaccatt
     2221 ttttgcatta tctcttaaac aaggttcttc atatcaatct cactagttta gatgttgctt
     2281 tgtctcgtga agagaggctt tatcaacttt tgatgtatct cttccccaag tatctacaag
     2341 ctgctattcg aaaaggtctt tataaggaat accagagatt ttctcataac gacagtcatg
     2401 ttaagggagt gattgatgta agaaaccatc tcaagaaaaa tcttcctttc acgggaaata
     2461 ttgcctacgc aacgagagag ttcacctatg ataatcccct catgcagttg gtccgtcaca
     2521 ctattgaata cattaagaat cagaaaagca ttggtcaagg ggtactagat aatctctcaa
     2581 ctagtcgtga aaacgtatct gaaatcgtgc gtgtaacgcc ctcttataaa ctagctgatc
     2641 gtgctaagat tattcgggga aatcaatcta aacctatacg tcatgcatac tttcacgagt
     2701 acagaaactt acaagaactt tgtctgatga tcctaaacca agaaaagcac ggtttagggt
     2761 atcaagatca aaaaatctat ggtattctct ttgatgttgc ctggctttgg gaagagtatg
     2821 tttacacctt gttgccaaaa ggttttgtac atcccagaaa taaggataag acggatggaa
     2881 tttcagtatt ttctgttggg aaacgaaaag tatatccaga tttttatgac agagaacgaa
     2941 agattgttct agatgcaaaa tataaaaaac tggaattgac tgaaaaagga atcaaccgtg
     3001 aggacttatt ccagctgatt tcctattctt atattttaaa agctgagaag gctggactga
     3061 tttttcctag tatggagcag tcagtaaata gtgaaatagg aaaagtagct ggctatggag
     3121 ctcaattgaa gaagtggtct attcgaatcc ctcagaatgc ctcattctat agtacatttt
     3181 gtaaaatgat ggaaaattca gaagaaaatt ttaaagcgat tattgatgaa gaagtgggga
     3241 gaaaataatt tcccctcttt cttttatttt taacacaagt aaaacccgaa cgatatattt
     3301 taattcataa aataatttga caaaaactgt actttgattt ataataaatt aaggaaacgt
     3361 cggaaaaggc gtagtgatgc aagagcatag gtaggtcatt acaaaagaaa cgagacatcg
     3421 atatgttaaa tgaatttcca atttttgatt acgaagatat tcaattgatt ccaaataaat
     3481 gtgtcattaa aagccgtgca gaagcggata caagtgtcac tttaggaaat cacaccttta
     3541 aactacctgt tgtgccagcg aatatgcaga cgattttgga tgaaaatgta gcagagcaac
     3601 tggctaaagg tggttacttc tacattatgc accgttttga tgaggcagga cgcattcctt
     3661 ttattaaacg aatgcacaat caagggctca ttgcttctat ctctgtcggt gttaaggatt
     3721 atgagtatga ttttgttagc cagctcaagg ctgatactcc tgagtatatc actattgata
     3781 ttgctcatgg tcatgcggat agcgtgattt ctatgattca acacatcaag aaagaattgc
     3841 cagatacctt tgtcattgct ggaaatgtgg gaacaccaga agctgtgcgt gaattggaaa
     3901 atgctggtgc ggatgctact aaggtcggaa tcggtcctgg taaggtttgt atcaccaagg
     3961 ttaagactgg ttttggtaca ggtggttggc agttggctgc tctacgctgg tgtgccaagg
     4021 ctgcgcgtaa accgattatc gctgatggag gaattcgtac tcacggtgat attgccaagt
     4081 ctatccgctt cggtgctagc atgatcatga ttggttccct ctttgcagga cacattgaaa
     4141 gtccagggaa aacgattgaa gtcgatggtg aacagttcaa agaatattat ggttcagcct
     4201 cacaatatca aaaaggtgct tacaaaaatg tggaaggcaa acgtatcttg cttcctgcta
     4261 aaggtcatct gcaagacact ttaactgaga tggaacaaga ccttcaaagt gctatttctt
     4321 atgcaggtgg acgtcaggtt gctgacctta aacacgttga ttatgtgatc gtgaaaaact
     4381 ccatctggaa tggggatgct tcccactaag acggggtctt tgaccaaaaa acttgttatt
     4441 agagcaaatt tctgttataa taaaacaagt ttccaccctt agtgtaatgg atatcacgta
     4501 agattccggt tcttgagatg ggggttcgat tccctcaggg tggatgtaaa catcctaaaa
     4561 agcctttaat agggcttttt tctttatcct gacccaaatt tgccgctaaa actgagaatc
     4621 tctgtctaaa gaattcccaa atgctttcca gtcccgtttt agagtgactc agggggcttt
     4681 ttttgatata ataaaaagga ctattatcag ttagaaagag gttggtatga aagaattaca
     4741 aactgtacta aagaatcatt ttgcaatcga atttgcagac aaaaatttac tggaaactgc
     4801 ctttactcat acgagttatg ccaatgagca ccgcctctta aaaatttcac acaatgaacg
     4861 cttggaattt ttaggagacg ctgttctaca gttattgatt tcagaatatc tatataaaaa
     4921 atatcctaaa aagcctgaag gtgacctatc aaaactccgt gctatgattg tccgtgagga
     4981 gagtttagct ggttttgcgc gtgattgcca gtttgaccag tttatcaagt tgggtaaagg
     5041 ggaagaaaaa tctggtggtc gcaatcgtga caccattctt ggtgatgcct ttgaagcctt
     5101 tcttggtgcc cttcttttgg ataaggatgt ggccaaggtc aaggaattta tctatcaagt
     5161 catgattcct aaggttgaag caggcgaatt tgagatgatt acagactata aaacccatct
     5221 ccaagagtta cttcaggtca atggtgatgt ggctattcgt tatcaggtga tttctgaaac
     5281 agggcctgct cacgataagg tctttgatgt agaagttctt gttgaaggta agagcatcgg
     5341 tcaaggccaa ggtcgttcta agaaattagc agagcaggaa gctgccaaaa atgccgttga
     5401 gaaggggctg gattcatgta tttaaaggaa atcgaaattc aggggttcaa gtcttttgct
     5461 gataagacca aggtcgtttt tgaccaaggt gtgacggcag ttgttggacc caatggatct
     5521 ggaaagtcca atattacaga aagtctgcgt tgggctttgg gggagtctag tgtcaagagt
     5581 ctccgtgggg gcaagatgcc ggatgtcatc tttgctggaa ccgaaagtcg caaaccgctc
     5641 aattatgctt ctgtagttgt gactctggat aatcatgacg gatttatcaa ggatgcaggt
     5701 caagaaatca gggtggaacg ccatatctat cgtagtggag atagcgaata caagattgac
     5761 ggcaagaaag tccgtctgcg tgatattcat gacctcttct tggatactgg attgggacga
     5821 gattccttct ctattatttc ccaagggaag gttgaggaga tttttaattc caagcctgag
     5881 gaacgacgag ctatttttga agaagctgca ggagttttaa aatacaagac tcgcagaaaa
     5941 gaaactgaga gtaaactgca acaaactcag gataatctag accgcttaga ggacattatc
     6001 tacgagttgg ataatcaaat caagcctctt gagaagcaag ctgagaatgc ctgtaagttt
     6061 ttagacttgg aaggacaacg taaggctatt tatttagacg ttctggttgc tcaaatcaag
     6121 gaaaataagg cagaactaga gtcgacagaa gaagagctgg ctcaggttca agaactcttg
     6181 atgagttatt accaaaagcg tgaaaaatta gaagaagaaa atcaaactct taaaaagcaa
     6241 cgccaagatt tacaggctga aatggccaaa gaccaaggca gtttgatgga cttgactagt
     6301 ctgattagtg atttagaaag aaaattagcc ctatcgaaac tggagtccga gcaagtggcc
     6361 ttgaatcaac aggaggcaca agctcgtttg gctgctttgg aggataagag aaattcactc
     6421 agcaaagaaa agtctgataa agaaagctct ttagctctgt tagagggaaa tctagtccaa
     6481 aataatcaaa aactcaatcg tttagaagct gaattgctgg ctttctcaga cgatcctgat
     6541 cagatgattg agctcttacg tgaacgcttt gtagctcttt tacaagaaga agcggatgtc
     6601 tcaaaccagt tgacccgtat tgagaatgag ttggaaaata gtcgtcagct ttctcaaaaa
     6661 caagcagatc aactagaaaa gctgaaagag caattagcta tagctaaaga gaaggctagt
     6721 cagcaaaagg aagagcttga aactgccaag gagcaggttc agaaattatt ggctgactac
     6781 caagctattg ccaaggagca agaggagcag aaaacttcct atcaagctca acaaagccaa
     6841 ctctttgacc gtctggataa tctcaaaaac aagcaggcca gagctcaaag tttggaaaat
     6901 atcctgagaa atcatagtaa cttttatgca ggtgttaaga gtgttctcca agaaaaagat
     6961 cgcctaggtg ggattattgg tgcagtcagt gagcatctga cctttgatat gcattatcaa
     7021 actgccctag aaattgcgct tggagcgagt agtcagcaca ttattgtaga agatgagaat
     7081 gcggcaacca aggctattga cttccttaaa cggaacagag ccggtcgtgc aacctttctt
     7141 cctttgacca ctattaaggc gcgtacgatt tctagtcaga accaagatgc tatcgctgta
     7201 agcccaggtt tccttgggat ggcagatgag ttggtgactt ttgatactag actggaagcc
     7261 attttcaaga acttgctagc tacgacggct atctttgata ccgtagaaca tgcgcgtgaa
     7321 gctgctcgac aagttcgtta tcaggttcgt atggtgacat tggatgggac agaattacgc
     7381 acgggtggtt cctatgcggg tggtgccaat cgccaaaata acagtattgt catcaagcca
     7441 gaactggagc aattacaaaa agaaattgct gcagatgaag caagcttggg ttcagaagaa
     7501 gcggctttga agaccttgca agaccagatg gctgcattga cagaaagatt agaagccatc
     7561 aaatctcaag gagagcaggc acgtattcag gagcaaggct tgtccctcgc ttatcagcaa
     7621 actagtcagc aagttgaaga actggaaact ctttggaaac tccaagaaga ggaaatagat
     7681 cgtctttctg agggagattg gcaagcggat aaggaaaaat gtcaagagag ccttgctact
     7741 atcgccagtg aaaagcaaaa tctggaagct gagattgaag aaattaagtc taataaaaac
     7801 gccatccaag aacgctatca aaatttgcag gaagaggtgg cgcaagctcg cttgcttaag
     7861 acagaactgc aagggcaaaa acgttatgaa gtagctgata ttgagcgttt aggcaaggaa
     7921 ttggacaatc ttaatatcga acaagaagaa attcagcgca tgctccaaga aaaagttgac
     7981 aatcttgaga aggttgatac agaattgctc agtcaacagg cggaagaatc caaaactcag
     8041 aaaacaaatc tccaacaagg tttgattcgc aagcagtttg agttggatga tatagaaggt
     8101 caactggatg atattgccag tcacttggat caagctcgcc agcagaatga ggagtggatt
     8161 cgcaagcaaa cacgtgctga agccaagaaa gaaaaggtca gcgagcgctt gcgccatcta
     8221 caaaatcaat taacagacca gtaccagatt agctatactg aagcactaga aaaggcacat
     8281 gaattggaaa acctcaatct ggcagagcaa gaggtgcagg atttagagaa ggctattcgc
     8341 tcattgggac ctgtcaactt ggaagctatt gaccagtacg aagaagttca caaccgtctg
     8401 gactttctaa atagtcagcg agatgatatt ttgtcagcga aaaatctgct ccttgaaacc
     8461 attacagaga tgaatgatga ggtcaaggaa cgctttaaat caacctttga agctattcgt
     8521 gagtccttta aagtgacctt caagcagatg tttggcggag gtcaggcaga cttgatattg
     8581 actgagggcg accttttaac agctggtgtg gagatttctg ttcaacctcc aggtaagaaa
     8641 atccagtcgc ttaacctcat gagtggtggt gaaaaagctc tatcggctct tgccttgctt
     8701 ttctccatta ttcgtgtcaa gaccattcct tttgtcatct tggatgaggt ggaagctgcg
     8761 ctggatgaag ccaatgttaa acgttttggg gattacctca accgctttga caaggacagc
     8821 cagtttatcg ttgtaaccca ccgtaaggga accatggcag cggctgattc catctatgga
     8881 gtgaccatgc aagaatcggg tgtttcaaag attgtttcag ttaagttaaa agatttagaa
     8941 agtattgaag gatgacaatt aaactagtag caacggatat ggacggaacc ttcctagatg
     9001 ggaatggacg ctttgatatg gatcgtctca agtctctctt ggcttcctac aaggaaaaag
     9061 ggatttactt tgcggtggct tcgggtcggg gatttctgtc tctggaaaaa ttatttgctg
     9121 atgttcgtga tgacattatt ttcatcgcgg aaaatggcag tttggtagag tatcaaggtc
     9181 aggacttgta tgaagcgact atgtctcgtg acttttatct ggcaactttt gaaaagctga
     9241 aaacgtcacc ttatgtagat atcaataaac tgctcttgac gggtaagaag ggttcatatg
     9301 ttctagatac ggttgatgag acctatttga aagtgagtca gcattataat gaaaatatcc
     9361 aaaaagtagc gagtttggaa gatatcacag atgacatttt caaatttaca accaacttca
     9421 cagaagaaac gctagaagct ggtgaagctt gggttaatga acatgtccct ggtgttaagg
     9481 ccatgacaac tggctttgaa tctattgata ttgttctgga ctatgtcgat aagggtgtag
     9541 ctattgttga attagctaaa aaacttggta tcacaatgga tcaggtcatg gcttttggag
     9601 acaatcttaa tgacttacat atgatgcagg ttgtgggaca tcctgtagct cctgaaaatg
     9661 cacgacctga aattttagaa ttagcaaaga ctgtgattgg tcaccataag gaacggtcgg
     9721 ttatagctta tatggagggc ttataatggc agatataaaa ttgattgcat tggacttgga
     9781 cgggaccttg ctgactactg ataaaaggct gacggatcgt accaaggaaa ccttgaaagc
     9841 tgcgcgtgat cgtggtatca aggtcgtatt gacaactggt cgtcccttaa aagccatgga
     9901 cttctttctc catgagttag ggactgacgg ccatgaagat gagtatacca ttacttttaa
     9961 tggtggatta gttcagaaaa atacaggaga aatccttgat aaaacagtct tttcatatga
    10021 tgatgtggca cgtttgtatg aagaaacaga gaaattatca ctgcctcttg atgccatctc
    10081 agaaggaaca gtttatcaaa tccaatcgga ccaagaaagt ctttatgcca aattcaatcc
    10141 agctttgacc tttgttccag tggactttga agacttatct agtcaaatga cctacaacaa
    10201 atgcgtgact gcctttgctc aagaaccctt ggatgcagcc attcagaaga tttctccaga
    10261 attgtttgac caatatgaaa tctttaaatc acgtgaaatg ttgctagaat ggtcaccaaa
    10321 gaatgttcat aaagcaacag gtttggcaaa actaatcagc catcttggaa tcgaccaaag
    10381 tcaagtgatg gcttgtggtg acgaggccaa tgacctctct atgattgaat gggcaggtct
    10441 tggtgttgct atgcaaaacg ctgtttctga agtaaaggca gccgcaaatg tagtgacgcc
    10501 gatgaccaac gatgaggaag ctgtcgcctg ggctatcgaa gaatatgtgc taaaggagaa
    10561 ctaagatatg ggattgtttg accgtctatt cggaaaaaaa gaagaaccta aaatcgaaga
    10621 agttgtaaaa gaagctctgg aaaatcttga tttgtctgaa gatgttgatc ctaccttcac
    10681 agaagttgag gaagtttctc aggaagaagc agaggttgaa attgttgaac aagctgtgtt
    10741 ccaagaagag gaaatccaag acacagttga agaaagtctg gatttagagc cagttgtaga
    10801 agtttctcaa aaagaagtcg aagaatttcc acactcagaa gaagggaata ctgagtttct
    10861 agagactata gaagaaaata attctgaagt tcttgaagca gaaaggcctc aagcagaaga
    10921 aaccgttcag gaaaaatatg accgcagtct taagaaaact cgtacaggtt tcggtgcccg
    10981 cttgaatgcc ttctttgcta acttccgctc tgttgacgaa gaatttttcg aggaactgga
    11041 agaactgctg attatgagtg atgttggtgt ccaagtcgct tctaacttaa cggaggaact
    11101 acgttacgaa gccaagcttg aaaatgccaa gaaacctgat gcacttcgtc gtgtcatcat
    11161 tgagaaattg gttgagcttt atgaaaagga tggtagctac gatgaaagca tccacttcca
    11221 agataacttg acagttatgc tctttgttgg tgtgaatggt gttgggaaaa caacttctat
    11281 cggaaaacta gcccaccgct acaaacgagc tggtaagaag gtcatgctgg ttgcagcaga
    11341 taccttccgt gcgggtgcag tagctcagct agctgaatgg ggccgacgag tagatgttcc
    11401 agtagtaact ggacctgaaa aagctgatcc agccagcgtg gtctttgatg gtatggaacg
    11461 tgccgtggct gaaggtatcg atattctcat gattgatact gctggtcgtc tgcaaaataa
    11521 ggataacctt atggctgagt tggaaaagat tggtcgtatt atcaaacgtg ttgtgccaga
    11581 agcaccacat gaaaccttct tggcacttga tgcatcaaca ggtcaaaatg ccctagtaca
    11641 ggccaaagaa ttttcgaaaa tcacaccttt aacgggaatt gttttgacta agattgatgg
    11701 aactgctcga ggaggtgtgg ttctagccat tcgtgaagaa ctcaatattc ctgtaaaatt
    11761 gattggtttt ggtgaaaaaa tcgatgatat tggagagttt aactcagaaa actttatgaa
    11821 aggtctcttg gaaggcttaa tctaatcaga agcaaaaatc ctgcaaggta caaacttgca
    11881 ggattttttt attcgaaacg accatcttga cgataggcga tatctggttg ccaagtccat
    11941 ttggcatcga atttttcaag taggtcaaag ctggcttgag gtcccatgct tccagcttta
    12001 tagtcatgaa gtggggcacc attttcggcc cagagctctt caatacggtc aatcaacttc
    12061 catgacgcac caacttcatc ccagtggcta aagttagttg agttgttatt taggacatca
    12121 taaatcaatt tttcgtatgg ttctggagaa gcaccagttg cagtcgcatc tgtacggtaa
    12181 tcaagtgagt taggagccaa gttaaattct tctcctactt gcttcccatt taggctaaga
    12241 gagaagcctt ctgttggttg aatatagatg gtcaaaatat ttggagcaag tggttctcca
    12301 aagatagaat ccatttgttt aaagacgatg ttgacatggg ttcctttttc agtcagtcgt
    12361 ttacctgtac ggaaaaagaa aggaacacca cggaatcgat cgctgtctac aaagaaggca
    12421 cctgatgtaa aggtttcagt tgttgattct ggattcacat ttggctcgct acgataagag
    12481 atgtatttca tgccatcaat cttaccagag cggtattgcc cacggataaa gtgttctttg
    12541 agttcttcat cagttggatg atagaggttt ttaaagacct taatcttttc agcacgaatc
    12601 tcgtcttttg tgaagcttgc tggtttgtcc atggcgagga gcgaaagaag ttgtagagtg
    12661 tggttttgga ccatgtcacg gagggcaccg gattggtcat agtagccacc acgttcttct
    12721 acacccaagc gctccgcaaa ggtaatttga acattgtcga taaaatcctt gttccaaacg
    12781 ttttcaaaaa tcaagtttgc aaagcgaact gcaaagatgc tttggatcat ttccttacca
    12841 agataatggt cgatacggaa aatttgttct tcgtcaaatg ttgctaggag ttcgtcattc
    12901 aacttgtttg cagttgcgta atctgtacca aatggttttt caacgatcaa gcgctcaaaa
    12961 cctttgccat cgacaatgtt ttcagatttg aggtgtttgg caatggttcc aaagaactga
    13021 ggtgccatag acaagaagaa gagcttattg tgttcagctt ggtatttttc attaagctca
    13081 gcttgtaatt gacgcaaagc aatataatgt tccgaatcat tgacatcatg gctttgatag
    13141 tagaagtggc tagcaaattc ttgggcttgc tcggtactat ctgccaaatc aaggatggac
    13201 tcgacaacta cagattcaaa atattcctta ctccaaggtc tacgggcagt tccaataacg
    13261 gcaaagtgct tggaaagatt gccggattta tatagtctaa aaagggaagg gtagagcttg
    13321 cgtttagcca ggtctccact cgcaccgaaa attgtaacaa taaccttaga tgacatctag
    13381 ctacctaatt tctattttta ttcctagtct tgctaggtat gaggaaacct catttcataa
    13441 acttaattct aacataattt tccgaaaaat caaaaattcc aatacgaggt agaaaaactg
    13501 caaggaaatc cttacagttt gagtttagac gtttaagacc ttatctaaga actctttcag
    13561 acgagggtgt tgtgggttat caaagatttg gtcaggtgtt ccgtcttcaa ggaactcgcc
    13621 atctgcagta aagataacgc ggttggcaac ctgacgagca aatcccatct catgggttac
    13681 gataatcatg gtcatgcctt gctcagccaa ttccttcata acgttaagta cgtctccaac
    13741 catctcaggg tcaagggcag aagttggttc atcgaagagc atgatgtctg gattcattgc
    13801 taggccacga gcgatggcca cacgttgttt ttgaccacct gataggctat ctggattggc
    13861 attagcttta tctgctagtc caaccttttc aagcaactcc attcccaatt cctcagcttc
    13921 ttccttagtc atcaacttgt gctcaatagg agcaaaggtg atgttgtcca atacagacat
    13981 atgagggaag aggttgaagt gttggaatac catgccgata ttttcacgga cgtggtcaac
    14041 attggttgtt ttttcagtta aatcatagcc gttcacagtg atgtgaccgc tagtgacttc
    14101 ttctaaaaga ttgaggctac ggaggaaagt tgacttacca gaacctgaag gaccgatgat
    14161 acaaacaaca tctccttcat agaacttagt cgtaattcct tttaggactt cattttttcc
    14221 atagtgcttg tgtaaatcat ttacatcaat ttttaatttt gccattaacg aatcctcttt
    14281 tctaagcgtt tcgctagtct agtcaaaagt gtgataatta caagatagaa gatagcaagg
    14341 attgcataca tcttgaaact ttggtagtta cgagcaatga taatcttacc agtttggaag
    14401 agttcaacca aaccgatagc agatacgata gttgtatctt taagagcgat aacgaattgg
    14461 ttgacaaagt ttggcaacat caatttagtt gcttgtggca agataatctt acgcatggtt
    14521 tttccataag agatacccaa gcttcggctg gcttccattt ggccaactgg aacggcctga
    14581 ataccaccac gaacgatttc agcgatataa gcagccgcat tgagtgagag ggcaatggtt
    14641 ccagctacaa agtcgttaat tgggctttgt tggcctgtga tagactcgat gaagtttgga
    14701 attccccaga agatgaaggc tgcaagaatc atcaatggaa taccacgaat aacgtcaacg
    14761 aaaatctcag agatgacgcg aagagatttg tatgggctaa cgctaaacat accgaagata
    14821 attccgatga caatggcaat agcaaatgag ataagagcta gagcaagagt gataccaaga
    14881 ccgctaagga gttgtttgta gttgttttga agcaagcccc agagcgttgt ttcgtcaaca
    14941 gtacttgttg aagcagttga agattcgcta gctaggtatt tgtcaagaat cttttggaat
    15001 tcaccgtttg ccttaaggtt tgcaagtccg ttgttgaaca tttcaatcag ttctggattt
    15061 gctccttttt taacggcaaa ggctgtttca ccgattggag ttccagagat tggagttttc
    15121 aatttttgac cttggctgat agaatatttg agaacaggtt catcatccat aacggcatca
    15181 atggcaccag tgtttaaact gtcatacatt gaagaaccat cagcaaaggt tttgattttg
    15241 taaccgtatt tgctttgatt ttctgttagg aaggtttgag aagcagttcc gtttttaaca
    15301 ccgactgtct ttccttttag atcttcataa gaagcaatat tgcttgattc tttgacacca
    15361 agaatggtat tagcagtgta gtatgattct gagaagtcaa aagttgcctt acgagcatct
    15421 gtgacagaca taccagcaat gataccatcg gcttgaccag cttggacagc actgatagca
    15481 gcatcaaaac cagggttggt gatttcaatt tcaaaacctt ggtctttagc gattgcctta
    15541 atcaattcca tatcaatacc agtgtattgg ttgcttgaat tttggaaaac aaaaggtgca
    15601 aaagaagaat cgctggcaat gatatattta gccttaacag gaatagcttt taatcctgcg
    15661 agagtagttg tagttggcac tgctgaagat gatgaagcag tccatttctt gataatttta
    15721 tcaagactac catctttttt catttcagcc aaggcttggt taaattcagt aaccaggtgc
    15781 tcgtatttac ttcctttttt cacaccgaaa gcaaaacttc ctacagcttc accatccatt
    15841 tcaatatgga ggtcttgacc ttggttaatg gcatattcga taacaggttt gtcatccatc
    15901 atggcatcga tggcaccagc actcaagctg ttgttcatta aatcaccagt gtcaaatgtt
    15961 ttaatagtaa agccgtattt atctttgatt gtttcaagga aacgttgagc ggcagttccg
    16021 tttttaacac caacggtttt gccagttaat tggtcgtact tgctaatttt gtgtgacttt
    16081 gtagtagcaa tgacaacttt tgtatcatag taagtatcag acatggtgaa gactttttca
    16141 cgttctttag tctttgtcat ccctgccatg atagcgtcgg cttgcccagc ttgaaccgca
    16201 ttgaccgctg cgtcaaatcc aggataggac atctgaatgt tccagccttt aatctcagcg
    16261 actttgttaa taatgtcaac atcaattcct ttataagttt gatctgaatc tttaaactca
    16321 aaaggtgcat aggcggtatc agaaacaatc ttaatcgttt ctgctttggc aatacctaat
    16381 gagaaaattg ggaataaaat tagcaaaaat gctagaaatt ttttcttcat ttttagtctc
    16441 cttttccgaa tattctctca ttatatcaga aaaagtaaaa aaaggcaaat ttttatattt
    16501 tccgtgtcag aaaatataac attgattttt ccttttc
//
LOCUS       DAVLRT010000040        16003 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLRT010000040 DAVLRT010000000
VERSION     DAVLRT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.040"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            374..757
                     /locus_tag="VXM85_001289"
     CDS             374..757
                     /locus_tag="VXM85_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1345863.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            809..1498
                     /locus_tag="VXM85_001290"
     CDS             809..1498
                     /locus_tag="VXM85_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1345864.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1540..1773
                     /locus_tag="VXM85_001291"
     CDS             1540..1773
                     /locus_tag="VXM85_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345865.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFLGAGYVVGFWLLILNENQRAN"
     gene            1924..2535
                     /locus_tag="VXM85_001292"
     CDS             1924..2535
                     /locus_tag="VXM85_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1345866.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFVPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYKSWPLLYYL"
     gene            2696..3490
                     /locus_tag="VXM85_001293"
     CDS             2696..3490
                     /locus_tag="VXM85_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1345867.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETVPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3487..4122
                     /gene="trmB"
                     /locus_tag="VXM85_001294"
     CDS             3487..4122
                     /gene="trmB"
                     /locus_tag="VXM85_001294"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1345868.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4248..4727
                     /gene="rimP"
                     /locus_tag="VXM85_001295"
     CDS             4248..4727
                     /gene="rimP"
                     /locus_tag="VXM85_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1345869.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4771..5907
                     /gene="nusA"
                     /locus_tag="VXM85_001296"
     CDS             4771..5907
                     /gene="nusA"
                     /locus_tag="VXM85_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1345870.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5929..6222
                     /locus_tag="VXM85_001297"
     CDS             5929..6222
                     /locus_tag="VXM85_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1345871.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6215..6514
                     /locus_tag="VXM85_001298"
     CDS             6215..6514
                     /locus_tag="VXM85_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1345872.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6531..9323
                     /gene="infB"
                     /locus_tag="VXM85_001299"
     CDS             6531..9323
                     /gene="infB"
                     /locus_tag="VXM85_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1345873.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9574..9924
                     /gene="rbfA"
                     /locus_tag="VXM85_001300"
     CDS             9574..9924
                     /gene="rbfA"
                     /locus_tag="VXM85_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1345874.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            10203..10982
                     /locus_tag="VXM85_001301"
     CDS             10203..10982
                     /locus_tag="VXM85_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345875.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQDPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            10984..11166
                     /locus_tag="VXM85_001302"
     CDS             10984..11166
                     /locus_tag="VXM85_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345876.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(11509..11583)
                     /locus_tag="VXM85_001303"
                     /pseudo
     CDS             complement(11509..11583)
                     /locus_tag="VXM85_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11614..11847
                     /locus_tag="VXM85_001304"
     CDS             11614..11847
                     /locus_tag="VXM85_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1345877.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            11847..13181
                     /locus_tag="VXM85_001305"
     CDS             11847..13181
                     /locus_tag="VXM85_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1345878.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLLEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWIQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            13191..13445
                     /locus_tag="VXM85_001306"
     CDS             13191..13445
                     /locus_tag="VXM85_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1345879.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            13541..13936
                     /locus_tag="VXM85_001307"
     CDS             13541..13936
                     /locus_tag="VXM85_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1345880.1"
                     /translation="MSKKLNRKKQLRNGLRRSGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            14348..14797
                     /locus_tag="VXM85_001308"
     CDS             14348..14797
                     /locus_tag="VXM85_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV1345881.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            14784..15341
                     /locus_tag="VXM85_001309"
     CDS             14784..15341
                     /locus_tag="VXM85_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1345882.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKVTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            15343..>16003
                     /locus_tag="VXM85_001310"
     CDS             15343..>16003
                     /locus_tag="VXM85_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1345883.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDINFTYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ET"
BASE COUNT         4879 a         2876 c         3635 g         4613 t
ORIGIN      
        1 ttaaccccgt tactgttcat tttaaatatt attggcctgt cttgcttttc agtgctaaca
       61 tacattaaca taaaagagat actcttaaat ataagtaaat gatgatttgg ttagtcctag
      121 aaccaatcaa cttatgttta tcggcttggc agattttatg tttgtcattt gtttgtatag
      181 aggtatttca gaaacagaat tctatcaaca actaatcgct tatataggtg tattctctgc
      241 ttgtttatca cgcttctgct catgtggggc ttgaagtatt ataaaaagta gctttctatc
      301 cttttctgac aggatatttg agtagaaggc tctattaaat tgttagtgaa ataaatgaat
      361 ggaggtattt aatatgaaat acagattatt ttttgttatt ttcttgagta gtgtgttgga
      421 tattctttta gggacatttt tacaaatctc tatcgtatct attgggtggc ttgttcttta
      481 cagtggtttg tttgaagcag gagttttcct tcttgctaat aaaggggtgg cggtaaaaat
      541 caaggaagta gatattcgaa atcgctttaa atttattttt ggaaaaacct tatggtttca
      601 aattcttttg ctcatctttt tgataatcaa actttatctt ggtttggatg cgaggttgat
      661 tttattctat ggacatattt tcattgtctt taatgcctta atgtatctat tatctagtag
      721 tcaggttagc cttaaaaaaa acaaactgtc ttcttaatct tacagcagta gagcctgaca
      781 aggtgaggtc atagtaggga ggatttctat gaaaaagtat caacttctat tcaaaataag
      841 tgcagtcttc tcttacttat ttttcgtatt tggtctttct cagctgacgc ttatcgtcca
      901 aaactattgg caattttctt ctcagatagg caatttattc tggattcaaa atatcttgag
      961 tttacttttt attggagtca tgattgtggt tcttgttaag acaggccatg gttatctctt
     1021 ccgcattcca agaaaaaaat ggctttggta ttcgattttg acagtattag tggtagtgtt
     1081 acagatctct tttaacgttc agacagctaa acatgttcag tcaactgcgg aaggttgggc
     1141 tgtattgatt ggttatagtg ggactaactt tgcagagcta ggtatttata tagccctgtt
     1201 ctttctggtt ccactgatgg aagaattgat ttatagagga ttactgcaac atgctttctt
     1261 taagcattcg cgatttggtc ttgatttgct tcttccttct attttatttg ctctccctca
     1321 tttttcaagc ctgcctagtc tgttagatat cttcgtcttt gcaacagttg gaatcatctt
     1381 tgctggtttg acccgctata ccaagagcat ttatccatcc tatgcggtgc atgtgatcaa
     1441 taatattgta gcgaccttcc cgtttttgct cacttttcta catagggtct tggggtaaaa
     1501 aaacaaaagg cttgcttttc agccatagag gaggtcatca tgtataaaca cttatttttc
     1561 ctagattcca aaactttaga tcggttgaca ccctatattc tagtcttggc ttctgacacc
     1621 attgctttta atgtttttgt gctaaccttt gtatctgcgg tggtttttaa tttcctaaat
     1681 tccatgctag ctttaatggc tatcttctta ggagctggct atgtggtcgg attttggtta
     1741 ctaatactca atgaaaatca aagagcaaac taggaaacta gccgcaggct gtacttgagt
     1801 acggcaaggc gacgttgacg tggtttgaat ttgattttcg aagagtataa aatggtttgt
     1861 tttggaaagg ctagaactaa aggatgacgt gtaggaagtc tgtttgttga ggaggataat
     1921 tttatggagt tttttgataa atttcatgcc ttgtgttttg gatttttagt actaataatt
     1981 gtcattacag ttccttatac gattaaccat ggggattttt ttcaaaatga atctgcattg
     2041 attattgtaa gtcttcttgt aacctcgctg agtgttgctt atgctagaaa gtttgaaatg
     2101 atttcttttg ggatgttaag caagaaacaa cttttgcttt tcattgcaat ctttcttcta
     2161 agtgtgcttg agacgctggt ttatattcat ttcttcgctg tttcttctgg ctcaggggtc
     2221 caacacttgg cggaagtcag cagaggaatt tccctgtctt tgattttgac tacctcagtt
     2281 tttgtcccca tccaggagga actcattttc agaggacttc ttcaaggtgc ggtttttgac
     2341 aattcttggt tagggcttgt gctaacttcc tctctctttt ctttcatgca tggaccttct
     2401 aatgtccctt cgtttatttt ttatctactt gggggcttgt tactgggctt tgcttataaa
     2461 aagagtcaaa acctatgggt ttctactcta gtacatatgc tttacaaaag ttggccactc
     2521 ttatattatt tataaaaatt atgaaaaggt aagcagaaga cggtgcttac ctttttcaag
     2581 ctataatgat acccaagcca atccagaggc ttttctttga gaatcgggtt agagtggttg
     2641 acgaataggc caaaaactag tagaatagta aggaaacttt atacggagga aagaaatgga
     2701 tttgggtgat aatgagctaa cactgactcc catacctggg aaaagtggca aggcttatat
     2761 gggtagctat cctgatggga agcgcatctt tgtaaaaatg aacacctctc caatcctacc
     2821 tggtctagct agagaacaaa ttgctccaca attattatgg agtcgtcgtt tggcagatgg
     2881 gcgtgatatg tgtgctcaag aatggttgac aggcaagata ttgaccccct atgatatgaa
     2941 tcgtaagcaa atcgtcaata ttttaacccg tcttcatcgc tcacgtccgt tgatgacaca
     3001 attgagtcgt ttgggctatg ccatggaaac acctgtagat ttactacagt cttggcagga
     3061 aacggttcca gatgctttgc gtaaaaatca ttttatcagt gaagtgatgg ctgatttacg
     3121 tcagactatt ccaggattta gagaggacca tgcgaccatt gtccatggag atgtacgaca
     3181 tagtaattgg attgagacag atagtggctt gatttattta gtagattggg attcggttcg
     3241 cttgaccgat cgcatgtttg atgtggccca tatgctctgc cattatattt cagaacatca
     3301 gtggaaggaa tggttgacct actacggtta caagtacaat caaacggtat taagtaaatt
     3361 gtattggtat ggtcaattgt cttatttgag tcagatttcc aagtattata tgaaccaaga
     3421 tttagaaaat gtcaatcggg agattcatgg tttgcgtcat ttccgagaca agtatggaaa
     3481 gagaagatga gagttagaaa tcgtaaaggg gcaacagaat tactagaggc aaatccccag
     3541 tatgtggtcc tcaatccctt ggaagccaag gcaaaatggc gggacttgtt tggcaatgat
     3601 aatcccattc atgtggaagt tggaagtgga aagggtgcct ttgtttcagg tatggccaag
     3661 caaaaccctg acatcaacta tatcgggatt gatattcaaa agtctgtttt gagctacgct
     3721 ttggacaagg tgcttgaagc tggagtgtct aacatcaagc tcttgtgggt agatggttct
     3781 gacttgactg actactttga agacggtgag attgatcgct tgtatctgaa cttttcagat
     3841 ccatggccga aaaaacgcca tgaaaagcgt cgtttgacct acaagacctt tttggatacc
     3901 ttcaaacgta tcttgcctga aaatggagaa attcatttca agacggataa ccgtggcttg
     3961 tttgagtaca gtttagtgag cttttctcaa tatggcatga aactcaatgg tgtctggcta
     4021 gatttgcatg ccagtgattt tgaaggcaat gtcatgacag aatacgagca aaaattctca
     4081 aacaaggggc aagttatcta ccgagttgag gcagaatttt aagagatagc ctaaaattag
     4141 gctgtacaag tgcttttgct ttacataagt tggcaaacgt gctatactga tagtaagaat
     4201 atgaaaagtg aggcggggaa atatcttcgc ctcttgctta tgaggaggtg gacgcaatcg
     4261 caacaatcgt agaattagtc agagaagttg tagaacctgt catagaagct ccttttgaac
     4321 tcgtggatat cgagtatgga aagattggca gtgacatgat tctcagtatt tttgtagata
     4381 aacccgaagg aattaccttg aacgacacgg cagacttgac agaaatgatc agtcctgtcc
     4441 tagacaccat caagccagat cccttcccag aacaatattt cctagaaatt accagtccag
     4501 gtttggaacg tcctttgaaa accaaggatg ccgtcgctgg agcggttgga aaatacatcc
     4561 atgtcgggct ctaccaagcc atcgataagc aaaaggtctt tgaaggaact ttgttggcct
     4621 tcgaagagga cgagttgact atggaatata tggacaagac gcgtaagaaa accgtccaaa
     4681 ttccatacag tttagtatca aaagcacgtt tagcagttaa attatagaaa aagaaaggat
     4741 agcttttgag gattcaaaag tgaagaaaac atgagtaaag aaatgctaga ggccttccgc
     4801 attttggaag aagacaaggg aatcaaaaaa gaagatatca tcgacgcagt agtagagtcg
     4861 cttcgttccg cttatcgcag acgctatggt cagtcagaca gcgtagctat tgacttcaac
     4921 gaaaaaacag gtgactttac agtttatact gtccgtgaag ttgttgatga agtatttgat
     4981 agccgtttgg aaatcagctt gaaagatgct cttgccatta attcagctta tgaacttgga
     5041 gacaaaatca agtttgaaga agcaccagct gagtttggtc gtgtagcagc ccaatctgcc
     5101 aaacaaacca tcatggaaaa aatgcgcaag caaacacgtg ccatcactta caatacttac
     5161 aaagaacatg agcaagaaat catgtctggt acagtagaac gctttgacaa ccgctttatc
     5221 tatgtcaacc ttggtagtat cgaagcccaa ttgtcaaaac aagaccaaat tcctggagaa
     5281 gtttttgctt ctcatgatcg tatcgaagtt tatgtttaca aggttgaaga caaccctcgt
     5341 ggtgtgaacg tctttgttag ccgtagccat ccagaaatga tcaaacgttt gatggagcaa
     5401 gaaattccag aagtttatga tggaactgtt gaaatcatga gcgtggctcg tgaagcaggt
     5461 gaccgtacga aggttgctgt tcgtagccac aatccaaacg tggatgctat cggtacaatc
     5521 gttggacgtg gtggtgctaa tatcaagaag attactagca aattccaccc agctcgttac
     5581 gatgctaaaa atgaccgcat ggtaccaatc gaagaaaata tcgatgttat cgagtgggta
     5641 gcagatccag ctgaatttat ctacaatgcc atcgctcctg ctgaggttga ccaagttatc
     5701 tttgatgaaa acgacagcaa acgtgccttg gtggttgttc cagataacaa gctttctctt
     5761 gccattggtc gtcgtggaca aaacgtgcgc ttggcggctc acttgactgg ttaccgtatc
     5821 gatatcaagt ctgctagcga atttgaagcc atggaagacg ctgcttcagt agagttggaa
     5881 gtagaaaacg atactgtaga agaataaaag ctgctagagg agggaaagat gaaaacaaga
     5941 aaaatccctt tgcgcaagtc tgttgtgtct aacgaagtga ttgataagcg tgatttgctc
     6001 cgcattgtca agaacaagga aggacaagtc tttattgatc ctacgggcaa ggccaatggc
     6061 cgcggcgctt atatcaaact agacaatgca gaagccctag aggcgaaaaa gaagaaggtc
     6121 tttaaccgca gctttagcat ggaagtggaa gaaagctttt atgacgagtt gatcgcttat
     6181 gtggatcaca aagtgaaaag aagagagttg ggacttgaat aagcaaaaga taagtaatct
     6241 cttggggctt gctcagcgag cagggcgcat catatcgggt gaagaattgg tggtcaaggc
     6301 cattcaagac ggcaaggcca agttggtctt tctagctcat gatgctggac ccaatctgac
     6361 caagaagatt caagataaaa gtcattatta tcaagtagaa attgtaaccg tgttttcaac
     6421 actggaatta agcatagcag tcgggaaatc gagaaaggtt ttggctgtaa cagatgctgg
     6481 atttacaaag aaaatgaggt ctcttatgga atagaagagg aggacatgat ttgtctaaga
     6541 aaagattgta cgaaatcgca aaagaacttg gaaaagaaag taaagaagtt gtagcgcgtg
     6601 caaaagagtt aggcttggat gtgaaaagcc actcatcaag tgtggaagaa gctgtcgctg
     6661 caaaaattgc tgccagcttt aagcctgcag ctgctccgaa agtagaagca aaacctgcag
     6721 caccaaaagt aagtgcagaa aagaaagccg aaaaatctga gccagctaaa ccagctgtag
     6781 ctaaggaaga ggcaaaaccg gctgagccag ttgctccgaa aacagaaaaa gtagcagtga
     6841 aaccgcaaag tcgtaatttc aaggctgagc gtgaagcccg tgctaaagag caggcagaac
     6901 gacgcaagca aaataagggc aataaccgtg accaacaaca aaacggaaac cgtcagaaaa
     6961 acgacggccg taatggtgga aaacaaggtc aaagcaaccg cgacaatcgt cgctttaatg
     7021 accaagctaa gaagcagcaa ggtcagcaaa aacgtagaaa tgagcgccgt cagcaagagg
     7081 ataaacgttc aaatcaagcg gctccacgta ttgactttaa agcccgtgca gcagccctaa
     7141 aagcagagca aaatgcagag tacgctcgtt caagtgagga acgcttcaag cagtatcagg
     7201 ctgctaaaga agccttggct caagctaaca aacgcaagga accagaggaa atctttgaag
     7261 aagcggctaa gttagctgaa caagcacagc aagttcaagc agtggttgaa gtcgtccctg
     7321 agaaaaaaga acctgcagtg gatacacgtc gtaaaaaaca agctcgacca gacaaaaatc
     7381 gtgacgatta tgatcatgaa gaagatggtc ctagaaaaca acaaaagaat cgaagtagtc
     7441 aaaatcaagt gagaaatcaa aagaatagta actggaataa caacaaaaag aacaaaaaag
     7501 gcaataacaa gaacaaccgt aatcagactc caaaacctgt tacggagcgt aaattccatg
     7561 aattgccaac agaatttgaa tatacagatg gtatgaccgt tgcggaaatc gcaaaacgta
     7621 tcaaacgtga accagctgaa atcgttaaga aacttttcat gatgggtgtc atggccacac
     7681 aaaaccaatc cttggatggg gaaacaattg aactcctcat ggtggattac ggtatcgaag
     7741 ccaaacaaaa ggttgaagtg gataatgctg acatcgaacg tttctttgtc gaagatggtt
     7801 atctcaatga agatgaattg gttgagcgtc caccagttgt tactatcatg ggacacgttg
     7861 accacggtaa aacaaccctt ttggatactc ttcgtaactc acgtgttgcg acaggtgaag
     7921 caggtggtat tactcagcat atcggtgcct accaaatcgt ggaaaatggt aagaagatta
     7981 ccttccttga tacaccagga cacgcggcct ttacatcaat gcgtgcgcgt ggtgcttctg
     8041 ttaccgatat tacgatcttg gtcgtagcgg cagatgacgg ggttatgcct cagactattg
     8101 aagccatcaa ccactcaaaa gcagctaacg ttccaatcat cgtagctatt aacaagattg
     8161 ataaaccagg tgctaaccca gaacgcgtta tcggtgaatt ggcagagcat ggtgtgatgt
     8221 caaccgcttg gggtggagat tctgaatttg ttgaaatctc ggctaaattc aaccaaaata
     8281 tcgaagaatt gttggaaaca gtccttcttg tggctgaaat ccaagaactc aaagcagacc
     8341 caacagttcg tgcgatcggt acggttatcg aagcgcgctt ggataaagga aaaggtgcgg
     8401 tcgcaaccct tcttgtacaa caaggtacct tgaatgttca agacccaatc gttgtcggaa
     8461 ataccttcgg tcgtgtccgt gctatgacca acgaccttgg tcgtcgtgtt aaagttgctg
     8521 gaccatcaac accagtctct atcacaggtt tgaacgaagc accgatggcg ggtgaccact
     8581 ttgccgttta cgaggatgaa aaatctgcgc gtgcagcagg tgaagagcgt gccaaacgtg
     8641 ccctcatgaa acaacgtcaa gctacccaac gtgttagcct tgaaaacctc tttgataccc
     8701 ttaaagctgg ggaactcaaa tctgttaatg ttatcatcaa ggccgatgta caaggttctg
     8761 ttgaagccct ttctgcctca cttcaaaaga ttgacgtgga aggtgtcaaa gtgactatcg
     8821 tccactcagc ggtcggtgct atcaacgaat cagatgtgac ccttgccgaa gcttcaaatg
     8881 cctttatcgt tggtttcaac gtacgcccta caccacaagc tcgtcaacaa gcagaagctg
     8941 acgatgtgga aatccgtctt cacagcatta tctacaaggt tatcgaagag atggaagaag
     9001 ctatgaaagg gatgcttgat ccagaatttg aagaaaaagt tattggtgaa gcggttatcc
     9061 gtgaaacctt caaggtgtct aaagtgggaa ctatcggtgg atttatggtt atcaacggta
     9121 aggttgcccg tgactctaaa gtccgtgtta tccgtgatgg tgtcgttatc tatgatggtg
     9181 aactcgcaag cttgaaacac tataaagacg acgttaagga agttacaaac ggccgtgaag
     9241 gtggattgat gatcgacggc tacaatgata ttaagatgga tgatgtgatt gaggcgtatg
     9301 tcatggaaga aatcaagaga taagattttt tgctcctttc ttaggtggtg agggacgcaa
     9361 gcaaaccgat ggtttcattg cttatttttg agcctagggt ctcaaaaata ccctgtgatg
     9421 ggactgataa atcagttcca tcactttcac cacggcgaaa gaagcagatg acttcaaatt
     9481 gaacttcgtt tcaatttaaa ctgaaaatca agaagtttaa aatagctagg tctgctggcc
     9541 tagcttttgg ttcaaagtag agaaaggaat atcatggcaa atcatttccg tacagatcgt
     9601 gtgggcatgg aaatcaagcg tgaagtcaat gagattttgc aaaagaaagt ccgtgatcca
     9661 cgtgtccaag gtgtgaccat cacagatgtt cagatgctgg gtgacttgtc tgttgccaag
     9721 gtttattaca ccattttgag taaccttgct tcggataacc aaaaagccca aatcgggctt
     9781 gaaaaagcaa ctggtaccat caaacgtgaa cttggtcgca atttgaaatt gtacaaaatc
     9841 ccagatttga ccttcgtcaa agacgagtcc atcgagtatg gaaacaagat tgacgagatg
     9901 ctacgcaatc tggataagaa ctaaagaaga ggggttgccc ctcttttttg gtggaggaaa
     9961 ataggttgaa tttgaaatgg aaaaatattc ttttataata gattgaaact agaatagtac
    10021 gcctctactt ctaaaacatt gttagaaatc gatttgactg tcctgatcga tttgtcctgt
    10081 tcttgtttca ttttactata aaaaaggggt tctgtatttt ttaatgttat ctaattagaa
    10141 aatgcttttt ttgtaggaaa tataatatga taaggtgcaa aaaagaaata aggagtttgt
    10201 atatggctga acaagactta gctatgcaag tattgcaaca agtggtgaaa ctacctgttg
    10261 ttaaggttga tcgttcgaaa tttttagtgg ataagttttc caaagaattg gatccaaaag
    10321 atattcctac cttattggaa caagacccaa cgactcttct atctcaagaa atattagatc
    10381 gtgtagctaa tgcttgtatt cgggacaatg tattattagc gagtgggact tctgttttgg
    10441 caggattacc tggagggctt gctatggcaa ttaccattcc agctgatgtg gctcaatttt
    10501 atgctttctc tctgaaattg gctcaagaat taggttatat ttatggttat gaggatcttt
    10561 gggcttcacg cgaggagttg agtgaagatg ctcaaaatac cctcttgctt tatctaggcg
    10621 taatgttagg ggtgaatgga accgctgctt tgctacgtgc tggtggtata acaattgcca
    10681 aacaggtaat gaaaacagtg cctaataaag ctttaacaaa gacgctttgg taccctattt
    10741 tgaaaaaagt cttaaaaata tttggtgtga atcttaccaa gggagggctg gccaaaggaa
    10801 tagggaaatt tattcctatc ttgggtggta tcatttcagg tggtttaacc tttgcaacta
    10861 tgaaaccaat gggggaaagc ttgcagaaag aattatccaa gctagtcaac tatagtgaag
    10921 ttcaatatca agaagatgtt gaaacaatcc gaaaagaggc tgaaatcatc aaaggagagt
    10981 aatatgaatc ctatcaaagc ttttgctaaa atttatggta attacttttt gaccgtgcaa
    11041 ggtgtaaaag tgatgaaaac gataaagaaa gctgaccatg tcgttgttgg tctgggcaaa
    11101 ctttttattg cagataagct aatggatacg gctcggtggc tcattaagcc agaggagaga
    11161 gaatgaaatt tttttggtct tcttgctatt ctttttatca aaccgattat tgggattgtg
    11221 aaattctttt ggatgatcat ttcttttgca gttcaattgc tgttttacaa gatattggat
    11281 tggttcttta aacttatcta gatggtaatc caagttgcag agaactagca ggaactccac
    11341 tgctagtttt ttattctctt tccatatggt ataatataag cagtaaaatc attttatact
    11401 cttcgaaaat ctcttcaaac cacgtcagct tcaccttgca gtatatatgt tactgacttc
    11461 gtcagttcta tccacaacct caaaacggtg ttttgagctg acttcgtcag ttctatctac
    11521 aacctcaaaa cactgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
    11581 cattgagtat tagaagatac aatggaggtc gtcatggaca atatcatcga tgtgtcaatt
    11641 cctgttgcag aagtggtgga caagcatcca gaagtcttgg aaattctagt ggagttgggt
    11701 tttaaacccc ttgccaatcc cttaatgcgc aatacagttg gtcgtaaagt atcacttaaa
    11761 cagggttcta agctagcagg aactcctatg gacaagattg tacgcacact ggaagcgaat
    11821 ggctacgaag tgattggatt agactaatga cagatgaacg gattcatatc ctacgggata
    11881 ttttgttaga attgcacaat ggcgcctctc ctgagtcggt tcaagatcgc tttgatgcga
    11941 cctttacggg cgtgtcagcc atcgagattt cccttatgga gcacgagctg atgaactcgg
    12001 attcgggcgt cacttttgaa gatgttatgg aactctgtga tgtccatgcc aatcttttta
    12061 aaaatgctat caaaggtgtc gaagttttag ataccgaaca ccctgggcac ccagttcgtg
    12121 tctttaagga agaaaatttg gccctccgtg cagctttgat tcgcattcgt agattgttag
    12181 atacctatga gttgatggaa gacgaggaaa tgctggcgga gatgcgcaag ggcttggtgc
    12241 gtcagatggg acttgtgggg caatttgaca tccattacca acgtaaagaa gaactcttct
    12301 ttcctatcat ggagcgctat ggacacgatt cacctcccaa agttatgtgg ggagtggatg
    12361 atcagattag ggaactcttt caaacagctc taacgacagc caagtcacta ctagaagtgt
    12421 caattagcag tgtaaaggaa gcttttgaag cttttgcgac agagtttgaa agtatgattt
    12481 tcaaggaaga gtccatcctc ctcatgattc tccttgagtc ttttactcag gatgactgga
    12541 ttcagattgc ggaggagagc gatgcctatg gctatgccat catccgtccg tcagagaaat
    12601 gggtgccaga acgacagagc tttattgagg aaaagattgc agaggagcct gtacagctag
    12661 atacggcaga aggtcaagtt caacaagtca tagatacgcc agaaggccat tttaccatta
    12721 cctttacccc taaggaaaag gaagctgtgc tggaccgcca tagtcaacag gcttttggta
    12781 atggctatct ttcagtcgag caggccaatc tcatcctcaa tcatctccct atggagatta
    12841 cctttgtcaa taaagaagat attttccagt attacaatga caatacgcca gctgatgaga
    12901 tgattttcaa acgaacgccg tcccaagtcg ggcgcaatgt cgaactctgt catccgccta
    12961 agtatttgga caaggtcaag gctatcatga agggacttcg tgaggggacc aaggacaagt
    13021 atgaaatgtg gttcaagtct gagtcgcgag gcaagtttgt ccacatcacc tatgctgcag
    13081 tacacgatga agacggagaa ttccaaggag tgttggagta tgttcaggat atccagccct
    13141 accgtgagat tgatacggac tatttccgtg gattagaata aggagaaaaa atgagttacg
    13201 aacaagaatt tatgaaggaa tttgaagctt gggtcaatac ccaaatcatg attaacgata
    13261 tggcgcacaa ggaaagtcaa aaagtttacg aagaagacca ggacgagcgt gccaaagatg
    13321 ccatgattcg ctacgagagt cgcttggatg cttatcagtt cttgcttggt aagtttgaaa
    13381 acttcaaagc aggcaaggga ttccatgatt tgccagaagg cttgtttggt gagcgaaatt
    13441 attaaacgag aaagattctt gatttttcac taaaatcttg atagaatgtt tatgttaaat
    13501 ccttgtcaga gcagggattt tttattgaaa ggattttatc atgtcaaaga aactcaatcg
    13561 taaaaaacaa ttacgaaatg gcctccgtcg ctcaggtgcc ttttcaagta ctgtgactaa
    13621 ggttgtagat gagacaaaaa aagtcgtgaa gcgtgcagaa cagtcagcaa gcgcagctgg
    13681 taaggctgtt tctaaaaaag ttgaacaagc agtagaagct accaaagagc aagctcaaaa
    13741 agtagctaat tctgtagaag attttgcagc aaacttgggt ggacttccac ttgatcgtgc
    13801 caagactttc tatgatgaag gaatcaagtc tgcttcagat ttcaaaaact ggactgaaaa
    13861 agaactcctt gacttgaaag gaatcggccc agctaccatc aagaaattga aagaaaatgg
    13921 catcaagttc aagtaatttt tcttgagcct tgcatttccg agaaaaactt gctacaatag
    13981 agccattaga ggtgttttga atcccacatt ttacagaaag tggcggcgct gagaagtcca
    14041 caaatgtgtc aaaactggtt gctaatgact gaaaaaatga aatatttctg tctttttatt
    14101 ttaaagacta aagatgcggg cttggcccga aattgggtgg taccgcggat aaacacattc
    14161 gtccctgtca tgtagatggc agggcttttc ttttgtgtct tagtcaaagg aggttgttat
    14221 aaaacaatca tttaaaacta ataaactata ttatggtttt cctgttttta ttttaggata
    14281 tcaagattag aattttggct ataacatcac gacctgtagt tcctcttata gtctaggaga
    14341 ttgggttgtg attggagtcg ttgcgagaga gaatgccgca gagcagatca aacagtatca
    14401 aaaatttact gtgaatattt ctgatgaaac ttctatgctt gcgatggagc aggctggttt
    14461 tatcagtcat caggagaaat tggaacgttt gggagtgcat tatgaaattt ctgaacgaac
    14521 tcagattcct attttagacg cctgtccact tgttttagat tgtcgggtag ataggattgt
    14581 tgaggaagac ggtatttgcc acatctttgc caagattctt gagcgacttg ttgccccaga
    14641 actcctggat gaaaagggac attttaaaaa tcaactgttt gccccaacct atttcatggg
    14701 agatggatat cagcgcgttt atcgctatct ggataagcgt gtagatatga agggcagttt
    14761 catcaaaaaa gcgaggaaga aggatggcaa gaactgagct gccagataaa atcgaaacag
    14821 aacgtctcgt tttacgagtc cgtactgtgg cggatgctga ggatatcttt gactatgcta
    14881 gtttgccaga ggtcgcctat ccagcaggtt ttcctccagt caagaccttg gaagatgaga
    14941 tttattatct ggagcacatt cttccggagc gtaatcaaaa ggaaaatctc ccagcaggct
    15001 acgggattgt cgtcaaagta acggataaga tcgttggctc tgtcgatttc aaccatcgcc
    15061 atgaagatga tgtgctggaa atcggctata ccttacaccc agactattgg gggcgaggtt
    15121 atgtgccaga agctgcgcgt gccttgattg acttagcctt taaagatttg ggtcttcaca
    15181 agattgaact aacttgcttt ggatataacc ttcaaagtaa acgagtcgcg gaaaagcttg
    15241 gctttaccct cgaagctcgc ataagagacc gaaaggatgt tcaaggaaac cgctgtgaca
    15301 gtctgatata tggcttgctg aagagcgagt gggaggaata agatgagcga tgtaaaagag
    15361 gaagtaagct ctctatctga gaagcagtta cgccaaattg atgttgagta tgctgaatta
    15421 aatgatagtg atattatcga gcgattagct tatttagaaa ttaacaacaa tgaaaaacgt
    15481 attgtcatct ctgatattga accaaccaag gaaataatgt cagtctcgga ccaaattttt
    15541 gagattcaaa agaattttca aaaaatcaag aatatgttcg aattgtttat ttcagatgtc
    15601 tcggattttt taagtattaa aaataaactt gagtcaaagg aattagaaat tgaagaggca
    15661 gatgtaaatc gtttcatgat tcatttatta tccagtggaa aattgtttgt cgattttaac
    15721 gaaaaccaaa ttaaacaaaa atattccaaa gatagtgagg agtttgattg tatacatggt
    15781 tttgcaagct atcagtatga tatcaatttt acttatcgat tttgccattc tttgagaaat
    15841 tattcgcaac atactgactt accaataaat gaggtaaaag ctgttagccc agatgatgag
    15901 actgttataa tagatttcta tattgattta gattatcttt taaattccaa ctttaaatgg
    15961 aaaaaattga aaggggagtt gataaaacta aaccaagaaa cat
//
LOCUS       DAVLRT010000041        15702 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLRT010000041 DAVLRT010000000
VERSION     DAVLRT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.041"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            187..1974
                     /locus_tag="VXM85_001311"
     CDS             187..1974
                     /locus_tag="VXM85_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1345884.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYIAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEERGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1971..2447
                     /locus_tag="VXM85_001312"
     CDS             1971..2447
                     /locus_tag="VXM85_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1345885.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2475..3380
                     /locus_tag="VXM85_001313"
     CDS             2475..3380
                     /locus_tag="VXM85_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1345886.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3367..4182
                     /locus_tag="VXM85_001314"
     CDS             3367..4182
                     /locus_tag="VXM85_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1345887.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4189..4593
                     /locus_tag="VXM85_001315"
     CDS             4189..4593
                     /locus_tag="VXM85_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1345888.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4892..6052
                     /locus_tag="VXM85_001316"
     CDS             4892..6052
                     /locus_tag="VXM85_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1345889.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATILVSFARSGNSPESVATVDLAKSLVDELYQVTITCATDGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEY"
     gene            6174..7211
                     /locus_tag="VXM85_001317"
     CDS             6174..7211
                     /locus_tag="VXM85_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1345890.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSITPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPVGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7418..8272
                     /locus_tag="VXM85_001318"
     CDS             7418..8272
                     /locus_tag="VXM85_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1345891.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIVQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            8422..8679
                     /locus_tag="VXM85_001319"
     CDS             8422..8679
                     /locus_tag="VXM85_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1345892.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8847..8975)
                     /locus_tag="VXM85_001320"
     CDS             complement(8847..8975)
                     /locus_tag="VXM85_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HEV1345893.1"
                     /translation="MDRTDEVSNHIYGKVKLMWFEEIFEEYKFLHNKTHITKVLMN"
     gene            complement(9009..9101)
                     /locus_tag="VXM85_001321"
                     /pseudo
     CDS             complement(9009..9101)
                     /locus_tag="VXM85_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9137..9433)
                     /locus_tag="VXM85_001322"
                     /pseudo
     CDS             complement(9137..9433)
                     /locus_tag="VXM85_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(9423..9584)
                     /locus_tag="VXM85_001323"
     CDS             complement(9423..9584)
                     /locus_tag="VXM85_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1345894.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            9654..10418
                     /locus_tag="VXM85_001324"
     CDS             9654..10418
                     /locus_tag="VXM85_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1345895.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            11031..12410
                     /locus_tag="VXM85_001325"
     CDS             11031..12410
                     /locus_tag="VXM85_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1345896.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIASRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12424..13089
                     /locus_tag="VXM85_001326"
     CDS             12424..13089
                     /locus_tag="VXM85_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1345897.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13203..13298
                     /locus_tag="VXM85_001327"
                     /pseudo
     CDS             13203..13298
                     /locus_tag="VXM85_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            13444..13983
                     /locus_tag="VXM85_001328"
     CDS             13444..13983
                     /locus_tag="VXM85_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1345898.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            14050..14460
                     /locus_tag="VXM85_001329"
     CDS             14050..14460
                     /locus_tag="VXM85_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1345899.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            14576..>15702
                     /gene="pavB"
                     /locus_tag="VXM85_001330"
     CDS             14576..>15702
                     /gene="pavB"
                     /locus_tag="VXM85_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1345900.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADG
                     LNPTPGQVLPEETPGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     SPSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLG"
BASE COUNT         4383 a         2995 c         3584 g         4740 t
ORIGIN      
        1 ctatattccg aatttaaaga gcgtttgaaa tgggtctaaa aatgtttgaa actaattgaa
       61 tgtgtgtata atagtaaaaa tgcagtaaat atcccgacaa tttctattga caattcaaac
      121 agattggttt ataattaata taacaacaaa tgaaagcgca aactttcgcg gtcggaaggt
      181 agttttatga cacgatttga gatacgagat gatttctatc tcgatggaaa atcatttaag
      241 attttatctg gtgccattca ttattttagg gttcctccag aggattggta tcattcgctc
      301 tataacttga aggctcttgg ttttaatacg gtagagactt atattgcttg gaatttacac
      361 gagcctcgtg aaggtgagtt tcattttgac ggtgatctgg atttagagaa atttctccaa
      421 atagcgcagg atttgggtct ctacgcaatt gtgcgtccgt ctccatttat ctgtgcggaa
      481 tgggaattcg gtggcttacc agcttggctc ttgaccaaga acatgcgaat tcgctcatcc
      541 gacccagcat atatcgaggc agttggtcgc tactatgatc agttattgcc aagactggtg
      601 cctcgtttgt tggacaatgg tggcaatatt ctcatgatgc aggttgaaaa tgagtatggt
      661 tcttacggag aagataaggc ttacctgaga gcgattcgac agctaatgga agagcgtggc
      721 gtaacctgtc ctctctttac atcagatggt ccatggcgag ctactctgaa agctggaacc
      781 ttaattgaag aggacctctt tgtaacagga aactttggtt ctaaggcacc ttacaacttt
      841 tcgcagatgc aggaattctt tgatgaacat ggtaagaaat ggccactcat gtgtatggag
      901 ttctgggatg gttggtttaa tcgctggaaa gaaccgatta tcacacggga tcctaaggaa
      961 ttggcagatg cagttcgaga ggttttggaa caaggctcta tcaatcttta catgttccac
     1021 ggtggtacaa actttggttt catgaatggt tgctcggctc gaggaacttt ggacctgcca
     1081 caagttacgt cttatgatta cgatgccctt ctggatgaag aaggaaatcc aactgctaaa
     1141 tatcttgcag tcaagaagat gatggcaaca catttttcag agtatccgca gttggaacca
     1201 ctctacaaag agagtatgga gttggatgct attccactag ttgaaaaagt ttctttgttt
     1261 gaaaccttag atagcttgtc aagtcctgta gaaagtctct atcctcaaaa gatggaggag
     1321 ctgggacaaa gttatggcta cctactttat cgaacagaaa caaactggga tgcagaagaa
     1381 gaaagacttc gtatcattga tggtcgagat agggcccagc tgtatgtcga tggtcagtgg
     1441 gttaaaactc aatatcagac agagattggg gaagatattt tttatcaagg taaaaagaaa
     1501 gggctatcta ggttagatat cttgatagaa aatatggggc gtgtcaacta tgggcataag
     1561 ttcttagcgg atacgcaacg taagggaatt cggacagggg tctgtaagga tctgcatttc
     1621 ttactaaact ggaaacacta tccacttcca ctagacaatc ctgagaaaat tgatttttca
     1681 aaaggatgga ctcaaggaca accagccttt tacgcttatg actttacagt cgaagagcca
     1741 aaagatactt acctagactt gtctgagttt ggtaagggag ttgcctttgt caatgggcag
     1801 aatctaggac gtttttggaa cgttggccca actctctcac tttatatccc tcatagctat
     1861 ctcaaggaag gtgccaaccg catcattatc tttgaaacag aaggtcaata taaagaagag
     1921 attcatttaa ctcgtaaacc tacactaaaa catataaagg gggaaaactt atgacaattg
     1981 taggatgccg tattgatgga cgtttgatcc acggacaagt agccaatctt tgggctggaa
     2041 aactaaatgt ttcacgcatt atggttgtag acgacgaagt tgtcaacaac gatattgaaa
     2101 agagtggttt gaaacttgcg acaccaccag gtgtgaaatt gagtattttg ccagttgaga
     2161 aagctgcagc caatattctt gctggtaaat acgatagcca acgtctcttt atcgtggctc
     2221 gtaaaccaga ccgcttcctt ggcttggtag aagcaggtgt accacttgaa acccttaatg
     2281 ttgggaatat gtctcaaaca ccagaaactc gttctattac acgttctatc aacgtagtag
     2341 acaaggatgt ggaagacttc cacaaactgg cagaaaaagg tgttaaactt actgctcaga
     2401 tggttccaaa tgatccaatt tcagactttt tgagcttatt aaaataggaa aaaaattttt
     2461 aggaggtcat tgttatgata caatggtggc aaattttact tctcactttg tactcagctt
     2521 atcaaatctg tgatgagttg acgatcgttt catctgcagg ttcccctgta tttgctggtt
     2581 tcattactgg tttaatcatg ggagatgtga ctactggttt acttatcggt ggtaacttgc
     2641 aactgttcgt tcttggggtt ggtaccttcg gtggtgcttc tcgtatcgac gcaacttctg
     2701 gtgcggttct tgcgacagcc ttctctgttt cacaaggaat tgatgcaccg cttgccatta
     2761 ctacaatcgc tgtaccagta gcagctctct tgacttactt cgacgttctt ggtcgtatga
     2821 ctactacctt cttcgctcac cgtgtggatg ctgcaatcga acgctttgac tataaaggta
     2881 ttgaacgcaa ctacttgctt ggtgcgattc cgtgggctct atctcgtgcc cttccagtct
     2941 tctttgccct tgcttttggt ggtgcctttg tacaatcagt agtagacttc gttgaagcct
     3001 acaaatgggt tgcagatggc ttgacacttg caggacgtat gcttccaggt cttggatttg
     3061 caatcttgct tcgttacctt ccagttaaac gtaaccttca ctaccttgct atgggatttg
     3121 gtttgacagc tatgttgact gttctttact catatgtaac aggtcttggt ggcgctgttg
     3181 ctggtatcgt aggtactctt cctgctgaag ttgctgaaaa aattggtttc gtgaacaact
     3241 tcaaaggttt gtctatgatt ggtatttcta tcgtaggtat tttccttgca gtgcttcact
     3301 tcaaaaatag ccaaaaagta gctgtagcag caccttctac accatcagaa agtggggaaa
     3361 tcgaagatga cgaattctaa ttacaaactt acaaaagaag attttaatca aatcaacaaa
     3421 cgtagcttgt ttactttcca attaggttgg aactacgaac gtatgcaagc ttctggttac
     3481 ctttacatga tcttgcctca gttgcgtaaa atgtatggtg atggaactcc tgaattgaaa
     3541 gaaatgatga aagttcatac tcaattcttc aatacttcac cattcttcca taccattatc
     3601 gctggttttg accttgccat ggaagaaaaa gatggtgtag gttcaaaaga cgctgttaac
     3661 ggtatcaaga caggtttgat gggaccattc gctcctcttg gggatacaat ctttggttca
     3721 cttgtacctg ctatcatggg gtcagtcgca gcaactatgg ctatcgctgg ccaaccttgg
     3781 gggatcttcc tttggattgc agttgcagta gcgtatgaca tcttccgttg gaaacagttg
     3841 gaatttgctt acaaagaagg ggttaacctt atcaacaaca tgcaaagtac cttgacagct
     3901 ttgattgacg ctgcatctgt acttggtgtc ttcatgatgg gtgctcttgt agcaacagtg
     3961 attaactttg aaatttctta caagttgcca atcggtgaaa agatgattga tttccaagac
     4021 atcttgaacc aaatcttccc acgtttgctt ccagcaatct ttactgcctt tatcttctgg
     4081 ttgcttggta agaaaggtat gaactctact aaagctatcg gtattattat cgtacttgct
     4141 ttggctcttt ctgcccttgg tcactttgca cttggaatgt aattccttat gactaaatca
     4201 ttaattttgg tgagccatgg tcgcttctgt gaggagctta gaggtagcac agaaatgatt
     4261 atgggcccac aagacaacat ttacacagta gctcttcttc cagaagatgg cccagaagaa
     4321 tttactgcta aatttgaagc tgttattgaa ggattggatg atttcctagt ttttgcggat
     4381 cttctcggtg ggacaccttg taatgtggtg agtcgcttga tcatggaagg tcgtgatatt
     4441 gacctttacg cagggatgaa tcttccaatg gtgattgaat ttatcaatgc gagccttaca
     4501 ggcgcagatg cggactacaa gagccgtgct gcagaaagca ttgtgaaagt taatgacctg
     4561 ttagcgggct tcgatgatga cgaagatgaa taatactctt cgaaaatctc ttcaaactac
     4621 gtcaacgtcg ccttaccgta ggtatatgtt actgacttcg tcagtcttat ccggcaacct
     4681 caaaacggtg ttttgagctg acttcgtcag tcttatccgg caacctcaaa gcagtgcttt
     4741 gagcagcctg cggctagttt cctacagatt ttagttggaa ctcgattcaa ttcatgtgac
     4801 aacgtgaaaa tcgttagagc attttatata gaatatacat gggaatggag cttactccca
     4861 ttcctatatt taatagaaaa agaggaactc aatgctacat tatacaaaag aagacttgct
     4921 cgaattgggt gcagaaatca ctacgcgtga aatctaccaa cagcctgatg tatggagaga
     4981 agcttttgaa ttttatcaag caaaacgtga agaaattgca gccttcctac aagaaatcgc
     5041 tgataaacat gactatatta aggttatctt gacaggtgct gggacttctg cttatgtggg
     5101 agacaccttg ctaccttact ttaaggaagt ctatgacgaa cgcaaatgga atttcaatgc
     5161 tattgcgaca acagatatcg ttgccaatcc agcaacctat ttgaaaaaag atgtggcaac
     5221 tatccttgtg tcttttgctc gtagtgggaa ctcgcctgaa agtgtggcga ctgttgattt
     5281 ggccaaatcc ttggtggatg agctttatca agtgacgatt acttgtgcaa cagatggtaa
     5341 attggctctt caagctcacg gtgatgaccg taatctcttg ctcttgcaac cagctgtctc
     5401 taatgatgct ggatttgcca tgacttctag ctttacgtct atgatgttga cagctctctt
     5461 ggtctttgat cctacagaat ttgctgttaa gtctgaacgt tttgaagttg tatctagtct
     5521 tgcccgtaaa gttttagaca aggcagaaga tgtcaaagag ctcgttgatt tagactttaa
     5581 ccgtgtcatc tatctaggcg ctggtccttt ctttggactt gctcatgaag ctcagctcaa
     5641 gattttggaa ttaactgctg gtcaagttgc gaccatgtat gaaagcccag ttggcttccg
     5701 tcacggtcca aaatctctta tcaacgacaa tacagttgtt ttggtctttg gtacaacgac
     5761 agactacact cgtaagtacg acttggactt ggttcgtgaa gttgctggtg accagattgc
     5821 tcgtcgtgtt gtgcttttga gtgatcaagc ttttggtctt gaaaatgtca aagaagtggc
     5881 ccttggttgt ggcggtgtct tgaatgatat ttaccgtgtc ttcccttaca tcgtttatgc
     5941 ccaactcttt gccctattga cttcactcaa ggtagaaaat aaaccagata caccgtctcc
     6001 tacaggtaca gtaaaccgtg tagtacaagg tgtcataatt cacgaatatt aaaagtaaga
     6061 cagtgtttat gaattcttga caagaggatt tgtaaattat cagataaacc atagattgtc
     6121 agtacgcttt ctatggtttg tttgcttgag agaaatagta aaaggagaac agaatgaaag
     6181 catacacaga gcgtgtattt ggaaatgttg agggcgagga tgtcttggcc tatcgatttg
     6241 agacagacgg tggctaccaa cttgaggtta tgacttatgg cgcgaccatc ttgcgctatg
     6301 tcgcacctga caaggctgga aattttgcca atgttatctt gggatttgat gactttgata
     6361 gttatgtagg caatagtccc aagcatggag caagtgtagg tcctgtagcg ggtcgtattg
     6421 caggtgcgac ctttgagctc aatggtaaga cctatgacct tgaggttaat aatgctagca
     6481 actgtaatca cagtggttca actggttggg attccagctt gtttgaagtt gaagaagtaa
     6541 gcgatcatgg cttgactctc tacacagaac gtacagatgg gacaggaggg ttccctggaa
     6601 atctcaagat ttggatcagt tatcacttgg aagaaactgg tgcctatgaa attagctaca
     6661 aggtaacgac cgatcaggat acgctggtca atccaaccaa ccacagctat ttcaacttgt
     6721 ctggtgattt cacgcagacg attgaccgtc atgtcttcca actaaacaca gagggcattt
     6781 actcaatcac tcctgacggt gttcctgcca aaactccaga agccaaccgt gatgtggtca
     6841 aacacatcta caatggtacc ttgttgaagg atatctttgc agaagaagat gagcaaatcc
     6901 agctggcatc aggtttggat cacccatttg cccttcctgt aggccatgac aatgctggat
     6961 tcctttatga ccaaaattca ggtcgcttcc tgcttttcaa gacagaagct ccttgctttg
     7021 tggtctacac agcaaacttt gtggatgaaa gtgtcatcat aggaggtcag ccaatgctac
     7081 agcacaatgg gattgctctt gaagcgcaag ctttaccaga tgccattcac agtgacctta
     7141 aaggccaagt cattcttaaa gctggtcaaa ccttcaccag taagacacgt tatgaacttg
     7201 ttgtgaaata aaaaagctgg tcaaaaagtc tttaaaatta aaataacgca tattgtcagg
     7261 tgatgaaaag tttgatacta tgcgttttat tatgaaatga tttactttat cttatcctga
     7321 aatggtgtta tttttgagtt atctggcttg actttcttga tggaaattat ataatagttg
     7381 taataacaat aattacaacg agaaaagagg accatttatg acctatgaat acaagagtca
     7441 catttatttg gcagagacag ttttaaatgt aaaggatttg gccagtcaaa cagcctttta
     7501 tcagcaagtt attggtttag aaatcttatc tcaaactgag acagagtcca ttctaggcct
     7561 tggtggaaaa gtcttggtac agctgattca agcacaagag agcggagaag tgagggaaca
     7621 ttatggtctt taccatttgg ccattctctt gcccacacgc aaggctttgg ctgatgtctt
     7681 gaaacacctg acagatttac agattcctct tgtcggcggt gcagaccatg gttacagtga
     7741 agctatttac ttggaagatt tggagggaaa tggcattgaa ctctatcgag ataagccagt
     7801 ttccacatgg gatattcgag aagatggacg cattatcgga gtgacggagg ctcttgcggc
     7861 gcaggacatt tatgagttgg gggaaagagt agagcccttt atcctggcag aaggtacaag
     7921 aatggggcat attcatcttt ccgtcaagga tagtcgaaag tccagccagt tttatcaaaa
     7981 ggtgttaggg ctagaggata aattcagtgt gcctagcgct agttggattg cggctggaga
     8041 ttaccatcat catttagcag ttaacgaatg gggaggaaaa ggtctggctt cgcgtaagca
     8101 aggcttacca ggtttagcct actatgtcat cgaagtcgca cataaagaag aactgttaac
     8161 gattgtccaa cgagcacaag aagttgacgc accaatcaaa tggatgacat cgagccaatt
     8221 ggaaatcaca gactcagatg gcatcgtgac ccgtattcgt ttagcaaggt aaaattagga
     8281 atttctaata agttataagt attgctagtt tgctttttga acggtctttg gtaacagaac
     8341 tgcgtgagct ggatgggtga gaattttgac aaagaagctg attagagata taatgaagaa
     8401 aaatcgagca aggaaaaaga aatgacaaat tcaaagtata ttacttgttt gaaacgttca
     8461 gagggccagt tgtgtgggat tcaaaagatg attgaaggag atcgtgactg tgctgatatt
     8521 gtgacgcaac tgacagcagt gagatctagt gtagagcgcg tgattgagat gataattacc
     8581 gaaaatctta ctgaatgtat caatcagcca ctagatgatt ctgaagctca aaaggaacgc
     8641 ctagaaaagg ctatccgata cttgattaaa cggaaataaa tgaatgtttt gagacgatat
     8701 gtgcaaaggt ctattaacta ttagaatagt ttaatgtgag tcaaaagaag ttgtaaaaaa
     8761 tctttaaaat taaaaaaacg catagtatca ggtgttgaat gtactgaccc ccaaaagtta
     8821 gattttttct gtctaacttt tgggggtcag ttcataagaa ccttggtaat atgcgtttta
     8881 ttatgtagaa acttatactc ttcgaaaatc tcttcaaacc acatcagctt caccttgccg
     8941 tagatatggt tactgacttc gtcagttcta tccacaacct caaaacagtg ttttgagctg
     9001 acttcgtcag ttccatccac aaccttaaaa cagtgttttg agcagcctgc ggctaacttc
     9061 ctagtttgca ctttaatttt cattgagtat tacttcatct tctcatgaaa ttgagttttt
     9121 gcccagctcc tcttgattat tctacttgac cgggccaagt attcatacca ccttctacgt
     9181 tggtaacggt gaggccttgg gagctgagaa attggcaggt agatgcagag cggactcctc
     9241 cttgacaaat aacatagtat tcatggtcag gtttgagttc tttgtagcct tgctctaagg
     9301 tacttaatgg aagatttttg gcacctggtg catgtcctgc ttggaattca tgtgcttcac
     9361 ggacatcgat aagttctaga ttttcatttt agtattttcc ataaaagtca gccatgctga
     9421 tactagtttc catattctac tccttctggg ttagccattt tgtatagtga ataagcgccg
     9481 tcaaggtttt ggacggtaaa tcctgcttgc ttgaggatag gctctgcgat atagctgcgc
     9541 aaaccactgt ggcagctgac gatgtaggct tgttttttgt ccagttcatc agcttcctcc
     9601 ttgacacttt gtcagttttt gatacaatag tacaaaatta gaggaggtgg gctatgattc
     9661 agaaacatgc gattcccatt ttagagtttg atgataatcc tcaggcggtt atcatgccca
     9721 atcacgaggg gttggatttg cagttgccaa agaagtgtgt ctatgctttt ttaggtgagg
     9781 agattgaccg ctatgcgagg gaagtagggg cgaactgtgt tggcgaattc gtttctgcta
     9841 ccaagaccta tccagtctat gtcatcaact acaagggtga ggaggtctgt ctggctcagg
     9901 ctcctgttgg ctccgctcca gcagcccagt ttatggattg gttgattggc tatggtgtgg
     9961 agcagattat ctctactggg acctgtggtg tcctagctga tatagaggaa aatgcctttc
    10021 tagtccctgt tcgcgctttg cgagatgagg gagccagtta ccactatgtg gcaccttgtc
    10081 gttatatgga aatgcagcca gaggctattg ctgctattga ggaagttttg gaagacagag
    10141 ggattcctta tgaagaagtc atgacctgga cgacagacgg tttttaccga gaaacggctg
    10201 aaaaggtggc ttatcgtaag gaagaaggct gtgctgttgt ggagatggag tgttctgctc
    10261 ttgcggcagt agctcaattg cgtggggttc tctggggtga attgttgttc acagcagatt
    10321 ctctagcgga cttggaccag tacgacagtc gtgactgggg ctcggaagct tttaataagg
    10381 cgctagaact gagtttagca agtgttcacc acctttagtt gtacaggcaa aggatttgtt
    10441 ttatcataaa atgtctagct catacttttc aaaaatatgt ttaaacgagg tcaccttcct
    10501 cttgtcctag gcatgttgag gttgggaaaa atctttaaaa tcagaaaaac gtatcatatc
    10561 aggtgatgaa aattttgata ctatgcgttt tatgttggta agatttagag tgagatgaaa
    10621 tgatactctt cgaaaatctc ttcaaaccag gtcagcttca ccttgccgta ggtatatgtt
    10681 actgacttcg tcagtcttat ccggcaacct caaaacggtg ttttgagctg acttcgtcag
    10741 ttctatccac aacctcaaaa cagtgttttg agcaacctgt gactagcttt ctaagcgatg
    10801 ccttggttct cattgcctat aatcaaaaag agaaattttc tcctgaaaag catatagagt
    10861 agctggcgtt aaaagctcct gtcttgcttt tttgacctat agtcacatct atcaagtatt
    10921 gttcttgcct aagctatcaa taaaaaggtg gcatttttta ggcttggtgt tagtgaattt
    10981 tgccttatcc tatctaagtc atttcgagct ttttatggta caatggaaac atgttattca
    11041 aattatctaa ggaaaaaata gagctaggct tatctcgttt atcgccagcc cgtcgtattt
    11101 ttttgagttt tgccttggtc attttactag gctctcttct tttgagcttg ccctttgtcc
    11161 aagttgaaag ctcacgagcg acttattttg atcatctttt cactgctgtc tctgcagtct
    11221 gtgtgacagg tctctcaacc cttccagtag ctcacaccta taatatctgg ggccaaataa
    11281 tctgtttgct cttgattcag atcggtggtc tagggctcat gacttttatt ggggttttct
    11341 atatccagag caagcaaaag cttagtcttc gtagccgtgc aactattcag gatagtttta
    11401 gttatggaga aactcgatct ttgagaaagt ttgtctattc tatttttctc acgacctttt
    11461 tggttgagag cttgggagct attttgctta gttttcgcct tattcctcaa cttggctggg
    11521 gacgtggtct ttttagttcc atttttctag cgatctcagc cttctgtaat gccggttttg
    11581 ataatttagg gagcaccagt ttatttgctt ttcagaccga tttactggtc aatctggtga
    11641 ttgcaggctt gattattaca ggcggccttg gttttatggt ctggtttgat ttggctggtc
    11701 atgtaggaag aaagaaaaaa ggacgtctgc actttcatac gaagcttgta ctattgttga
    11761 ctataggttt gttgttattt ggaacggcaa ctactctctt tcttgagtgg aacaatgctg
    11821 gaacgattgg caatctccct gttgccgata aggttttagt tagctttttt caaacagtga
    11881 cgatgcgaac agctggcttt tctacgatag attatactca ggctcatcct gtgactcttt
    11941 tgatttatat cttacagatg tttctaggtg gggcacctgg aggaacagct gggggactca
    12001 agattacgac attttttgtc ctcttggtct ttgcacgaag tgagcttcta ggcttgcctc
    12061 atgccaatgt tgcgagacga acgattgcgt cgcgaacggt tcaaaaatcc tttagtgtct
    12121 ttattatctt tttgatgagc ttcttgatag gattgattct gctagggata acagccaaag
    12181 gcaatcctcc ctttatccac ctcatatttg aaaccatttc agctcttagt acagttggtg
    12241 taacggcaaa tctgactcct gaccttggga aattggctct cagtgttatc atgccactta
    12301 tgtttatggg acgaattggt cccttgacct tgtttgttag cttggcagat taccatccag
    12361 aaaagaaaga tatgattcac tatatgaaag cagatattag tattggttaa gaaaggaaag
    12421 agcatgtcag atcgtacgat tggaattttg ggtttgggaa tttttgggag cagtgtccta
    12481 gctgccctag ccaagcagga tatgaatatt atcgctattg atgaccacgc agagcgcatc
    12541 aatcagtttg agccagtttt ggcgcgtgga gtgattggtg acatcacaga tgaagaatta
    12601 ttgagatcag cagggattga tacctgcgat accgttgtag tcgcgacagg tgaaaatctg
    12661 gagtcgagtg tgcttgcggt tatgcactgt aagagtttgg gggtaccgac tgttattgct
    12721 aaggtcaaaa gtcagaccgc taagaaagtg ctagaaaaga ttggagctga ctcggttatc
    12781 tcgccagagt atgaaatggg gcagtctcta gcacagacca ttcttttcca taatagtgtt
    12841 gatgtctttc agttggataa aaatgtgtct atcgtggaga tgaaaattcc tcagtcttgg
    12901 gcaggtcaaa gtctgagtaa attagacctc cgtggcaaat acaatctgaa tattttgggt
    12961 ttccgagagc aggaaaattc cccattggat gttgaatttg gaccagatga cctcttgaaa
    13021 gcagatacct atattttggc agtcatcaac aaccagtatt tggataccct agtagcattg
    13081 aattcgtaaa gagggatgac ccctcttttt tgatgcccaa gatggcaaat agagacagaa
    13141 gccccttgtc ttctagtaaa agttcttcaa aggctggact ttatggtaaa atagaaggaa
    13201 gtgacaagag agagtaatac tcaatgaaaa tcaaagatca aactaggaaa ctagctgcgg
    13261 gctgctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagc tcaaaacact
    13321 gttttgaggt tgcagataga actgacgaag tcagtaacat ctatacggca aggcgacgtt
    13381 gacgcggttt gaagagattt tcgaagagta taagaaaaaa tcagtcccct aaaggagcag
    13441 attatgaagt tattgtctat cgccatttct agctataatg cagcagccta tcttcattac
    13501 tgtgtggagt cgctagtgat tggtggtgag caagttggga ttttgattat caatgacggg
    13561 tctcaggatc agactcagga aatcgctgag tgtttagcta gcaagtatcc taatatcgtt
    13621 agagccatct atcaggaaaa taaatgccat ggcggtgcgg tcaatcgtgg cttggcagag
    13681 gcttctgggc gctattttaa agtagttgac agtgatgact gggtggatcc tcgtgcctac
    13741 ttgaaaattc ttgaaacctt gcaggaactt gagagcaaag gtcaagaggt ggatgtcttt
    13801 gtgaccaatt ttgtctatga aaaggaaggg cagtctcgta agaagagtat gagttacgat
    13861 tcagtcttgc ctgttcggca gatttttggc tgggaccagg tcggaaattt ctccaaaggc
    13921 cagtatacca tgatgcactc gctgatttat cggacagatt tgttgcgtgc tagccagttc
    13981 taactgcctg aacatacttt ttatgtcgat aatctctttg tctttacgcc ccttcagcag
    14041 gtcaagacca tgtactatct gcctgtcgat ttctatcgtt atttgattgg gcgtgaggac
    14101 cagtctgtca atgagcaagt gatgattaag tgcattgacc agcaactcaa ggtcaatcga
    14161 ctcttgatag accaacttga tttgtcccaa gtgagtcatc ccaaaatgcg agaatatctg
    14221 ctgaatcata ttgaactcac gacggtgatt tccagtaccc tgctcaaccg atctggaaca
    14281 gcggagcatc tggcaaaaaa acgccaattg tggacctata ttcagcagaa aaatccagaa
    14341 gtctttcagg ctattcgtaa gaccatgttg agccgtttga ccaaacattc tgtcttgcca
    14401 gatcgcaaac tgtccaatgt cgtctatcaa atcaccaaat ctgtttatgg atttaattaa
    14461 tataagtgtt ttataagagg gatttaagaa aaattttaac tttttcttag tcctttttaa
    14521 tttcaggaga ttatactaga gtcatcaaat aaagaaagac tctaaggaga atcctatgaa
    14581 attcaatcca aatcaaagat atactcgttg gtctattcgc cgtctcagtg tcggtgttgc
    14641 ctcagttgtt gtggctagtg gcttctttgt cctagttggt cagccaagtt ctgtacgtgc
    14701 cgatgggctc aatccaaccc caggtcaagt cttacctgaa gagacaccgg gaacgaaaga
    14761 gggtgactta tcagaaaaac caggagacac cgttctcact caagcgaaac ctgagggcgt
    14821 tactggaaat acgaattcac ttccgacacc tacagaaaga actgaagtga gcgaggaaac
    14881 aagcccttct agtctggata cactttttga aaaagatgaa gaagctcaaa aaaatccaga
    14941 gctaacagat gtcttaaaag aaactgtaga tacagctgat gtggatggga cacaagcaag
    15001 tccagcagaa actactcctg aacaagtaaa aggtggagtg aaagaaaata caaaagacag
    15061 catcgatgtt cctgctgctt atcttgaaaa agctgaaggg aaaggtcctt tcactgccgg
    15121 tgtaaaccaa gtaattcctt atgaactatt cgctggtgat ggtatgttaa ctcgtctatt
    15181 actaaaagct tcggataatg ctccttggtc tgacaatggt actgctaaaa atcctgcttt
    15241 acctcctctt gaaggattaa caaaagggaa atacttctat gaagtagact taaatggcaa
    15301 tactgttggt aaacaaggtc aagctttaat tgatcaactt cgcgctaatg gtactcaaac
    15361 ttataaagct actgttaaag tttacggaaa taaagacggt aaagctgact tgactaatct
    15421 agttgctact aaaaatgtag acatcaacat caatggatta gttgctaaag aaacagttca
    15481 aaaagccgtt gcagacaacg ttaaagacag tatcgatgtt ccagcagcct acctagaaaa
    15541 agccaagggt gaaggtccat tcacagcagg tgtcaaccat gtgattccat acgaactctt
    15601 cgcaggtgat ggcatgttga ctcgtctctt gctcaaggca tctgacaagg caccatggtc
    15661 agataacggc gacgctaaaa acccagccct atctccacta gg
//
LOCUS       DAVLRT010000042        15161 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLRT010000042 DAVLRT010000000
VERSION     DAVLRT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.042"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(231..1838)
                     /locus_tag="VXM85_001331"
     CDS             complement(231..1838)
                     /locus_tag="VXM85_001331"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1345901.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2228..2815)
                     /locus_tag="VXM85_001332"
     CDS             complement(2228..2815)
                     /locus_tag="VXM85_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1345902.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2943..3392)
                     /locus_tag="VXM85_001333"
     CDS             complement(2943..3392)
                     /locus_tag="VXM85_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1345903.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3397..3786)
                     /locus_tag="VXM85_001334"
     CDS             complement(3397..3786)
                     /locus_tag="VXM85_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1345904.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3814..4440)
                     /locus_tag="VXM85_001335"
     CDS             complement(3814..4440)
                     /locus_tag="VXM85_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1345905.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4454..5017)
                     /locus_tag="VXM85_001336"
     CDS             complement(4454..5017)
                     /locus_tag="VXM85_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1345906.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5182..5416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5470..5973)
                     /locus_tag="VXM85_001337"
     CDS             complement(5470..5973)
                     /locus_tag="VXM85_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1345907.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6166..6996)
                     /locus_tag="VXM85_001338"
     CDS             complement(6166..6996)
                     /locus_tag="VXM85_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1345908.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYLTLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6981..8663)
                     /locus_tag="VXM85_001339"
     CDS             complement(6981..8663)
                     /locus_tag="VXM85_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1345909.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQIQLQTELVKETPALQSLFILEEVSFSYNDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8765..9067)
                     /locus_tag="VXM85_001340"
     CDS             complement(8765..9067)
                     /locus_tag="VXM85_001340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345910.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     SKHGFEVVDETDEVSNHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9074..9622)
                     /locus_tag="VXM85_001341"
     CDS             complement(9074..9622)
                     /locus_tag="VXM85_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1345911.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9637..10485)
                     /locus_tag="VXM85_001342"
     CDS             complement(9637..10485)
                     /locus_tag="VXM85_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1345912.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10674..12023
                     /gene="trkA"
                     /locus_tag="VXM85_001343"
     CDS             10674..12023
                     /gene="trkA"
                     /locus_tag="VXM85_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1345913.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12027..13466
                     /locus_tag="VXM85_001344"
     CDS             12027..13466
                     /locus_tag="VXM85_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1345914.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13592..14050)
                     /locus_tag="VXM85_001345"
     CDS             complement(13592..14050)
                     /locus_tag="VXM85_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIB"
                     /protein_id="HEV1345915.1"
                     /translation="MEVVLFSYCLNTRYLLQVVDIASSNDIILEEDTSEIMPGEIDVI
                     KSKELKVLVLCAGSGTSAQLANAINEGANLTEVRVIANSGAYGAHYDIMGVYDLIILA
                     PQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            14609..14779
                     /locus_tag="VXM85_001346"
     CDS             14609..14779
                     /locus_tag="VXM85_001346"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1345916.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
BASE COUNT         4538 a         3416 c         2746 g         4461 t
ORIGIN      
        1 atccagttcc taaaactttg ctaaaaatta atttgacttc ccgaattaat ttgttcagat
       61 tttatttcat ttcaatatac tgataaatcc attcactagc acagggcaag agggagtccc
      121 tattgatttc aacggtagca ggtacataca gaggactcct acctcagata tactgcaata
      181 tcatcgtgtg caaaaaacct ctgagatttt tctcaaaggt tctgattttg ctaattgctg
      241 ttctcaaccg ctgcagtaac aaaggcagtg tagagttctt ctggtcggtt tggacggctt
      301 gacagttcag ggtgatactg acaagctaca aagaatttat tttcaggaat ttccacgatt
      361 tctaccaaac gattgtctgg agaaactcct gaaaagacaa agcctgctgc ctcaaactgc
      421 tcacggaagg cgttattaaa ctcataacgg tgacggtgac ggcgttgcac cacttcttga
      481 ttgtgataag cagcagcagc cttagagcca cgtttcaact tagacggata aagtcccaaa
      541 cgaagggttc cacccatatc ctcaatatca atctgatcac gcatgatatc aatgataggg
      601 tattttgttt ctggtgcaag ctctgcagaa ttggcacctt caagacctaa aacgtgacga
      661 gcaaactcga tacatgtcaa ctgcattccc aagcagactc ccaacattgg aacatcattt
      721 tcacgcgcat agcggatggc ttggattttc ccttctgtac cacgttgacc aaaaccacct
      781 ggtacgatga tcccgtccgc atcagacaag agttctgcta cattctctgc tgtcacatca
      841 ttggcattga cccaattgat tttaacttct acatcattga catagccaga gtgtttcaag
      901 gcttcgacca ctgagatata ggcatcttgc aactccacat acttaccaac aagggaaatc
      961 ttaacttgtt tcttgaggtt catgaccttg tccaccatgg ctgaccattc tgtcatatcc
     1021 gctgctggtg cgtctaattt caaatgatca caaacaattt ggtccatccc ttgtgcctgc
     1081 aagttcagtg gaatttggta aaggtgttca acatccaacg attcgataac ggcttctggt
     1141 gccacatcac agaactgggc cagtttattt ttaattcctt gaccagctgg ctcttctgta
     1201 cgaataacca acatatttgg ttggattccc aagccacgca attctttgac agagtgttgg
     1261 gttggtttgg ttttcatttc accagcagcc ttgaggtaag gaagcaaggt tgtatggata
     1321 tacatgacat tatccgcacc cacatctgcc ttcatctgac gaagagcctc taggaatggc
     1381 aaggactcga tatctcctac tgttccacca acctctgtga taatgacatc agagtcggtc
     1441 gttagagcgg cacgcttgat tttttctttc aaagcatctg tgatatgagg aatgacttga
     1501 acagttgccc caaggtattc tccacggcgt tctttacgaa gaacttcact gtaaattttc
     1561 ccagttgtca cgttggaata tttgttgaga ttgatatcga tgaaacgttc atagtgaccc
     1621 aagtccaaat ctgtctcagc tccgtcatct gtcacaaaaa cttccccgtg ctggtaagga
     1681 ctcatggttc ccggatcaat attgatataa gggtcaaact tttgaatggt tactttgaga
     1741 ccacgatttt tcaagagacg gcctagactc gctgccacaa tccctttccc aatagacgat
     1801 accacaccac cagttacaaa aatatatttc gtagacatag attcctcttt ctaaaatgct
     1861 caaggtcttg ttaactacga ggggatatag aaaacaagac cctactaata agaataatct
     1921 gggacgactg caccagattc acaactaata ctcttcgaaa atctcttcaa accgcgtcaa
     1981 cgtcgccttg ccgtagatat gtgttcctga ctttgtcagt cttatctaca acctcaaaac
     2041 agtgttttga gcagcctacg gctagtttcc tagcttgctc tttgattttc attgagtata
     2101 aaatacaaga agataacttc ttgaaaaaac aaaaatagct ccctaagaac tagggagccc
     2161 cgacctctaa aagaggtgcc caaacaatat gatacctaaa aataggataa ttgtcaatag
     2221 ctaactctta ttcctcgctg gtttcagcgt cgtcatctga aaactcgtcg tcgtcttcgt
     2281 tgagatccac atcttctccc aagtcatctg gagcgatttc gttgatttct gcatcataag
     2341 cttccacttc atttttttca tcatctggat tttcatcatc gtatgaaaga gctggatctg
     2401 cttcgtatgc atcttcgtct tctggatcat ctgcattgta gtcaatggca tctgaatcac
     2461 catccataaa ggcattgaca cgttttttct tagcttttgg tgctacttca tcgtcgtcat
     2521 tttcttcaag agcgatgatt tcttcgtcga tttcgtccac accataccat gaacgaagac
     2581 cccatttgtt gtccccaagt gagatgaagc taccgtcaaa gttcaactct gtgtagaaca
     2641 aaggcaaagc ttcgcggata tcgctgtttg atgttccaag gtagttttga atttcgttta
     2701 caagatcgct aaaatgcatc tcatgatcgc gaccacgaag ttctaatata gcacgcgcta
     2761 cctcaatcat agatagttca cttttttctt gcccagcaaa tacttctaat tccaaagcgt
     2821 ttctcctcat ttatactact atcgtcagag cgaacagact ctgacctcat tttatcattt
     2881 actctttatt ttacgataat tttgcggaat agtcaaaggt taagggggag aaagtggcag
     2941 gattagacta attccaatat aaaactcctt cctttttctg ttgctccatt ttccacaaat
     3001 ccaagcgact tgaaacacct cctagaagca tgattgtagg tgtagatttc cttgactctc
     3061 aattctttcc atccttttac tcgagccaat tcaatcaaag cacttagaat cttttttcca
     3121 agtcctcgat gttggtaagc ggaattccca atcacaatgg ggagattatc ctgagatagt
     3181 gtaatatccc caattggaaa ccattctccc ttctccttga cttcaatcca aaaaagctca
     3241 ccatgccgat ccaaatagga atacatggct tccaaggtcg cttgactgta aggaagcttc
     3301 accccatcta cgaggtaaac caagttcaca tcctgatacc aagcaaaagc ttcctcacaa
     3361 tgattgacct tataataagg aacaaaacac aatgcactac cttttcaaca aaagagttgc
     3421 tgcttgatta aaaccatcac accagttata ccattttgct tcatacccat cttgagctag
     3481 gatacgatct tctaaatcaa aaacagagta aatctttctt tcctcgcaag cttgcgcata
     3541 gagatgatat agttcatcac caccatctct atcccactca gcagaaatcg tatcccgacc
     3601 tgccaataaa gcctgataag ccctgtgatg cccatctgta atcagcaaac aatctccaaa
     3661 ggcaagaata ctgattggat cgacttggat tgtttctgcc gactggtaaa gcatctgaat
     3721 atcttgcaac ttcttttctg ataaatatag ttgagtcgga tgaagatctg ctatattgac
     3781 tttcatttct ttctcctcaa gggaattcga tactcacttc tgtttgcctt taaatcgcca
     3841 ttggaagcgg agcttgtcat agaagggaaa ctcgataaac aggactccca agcccacaca
     3901 gagactggca aggacgtctg atgggtaatg aactcccaga tagactcttg ataccagcac
     3961 actgactagg tagaggccaa ggacgatttg tacgattttt ctccagactg gatttttaat
     4021 tcgttgactg agaatgacaa tcagagtacc taccatcaaa gttacagcca gagaatgccc
     4081 acttgggaag gaaaatccct tctcttccac cagatgtaag atagctggtc gtgggcgctg
     4141 gtagatattt ttaaaggtca cgattaaaag acctgccaaa gccagatttc ccagcatgaa
     4201 gaaactttct atcttccatc gcttacgata aaagacaaaa gctgtaatga caacccaagt
     4261 gataatcact gggatatcaa tcagacgtgt gagggctcga aaaagaatag tcaaataatc
     4321 tggtaagtct cctcgaatgg cagtctgaat cgattggtca aaattgacca acatttcagg
     4381 gtaaaatttg accatatagc caagaataac gaaaagtaaa agggcaaaac tgcccttcat
     4441 taaaaatgtt tgtttatctt tcatagtgtt ttaaggttgg tttcaaaagg acgtacaaca
     4501 accagaatga aacggaaaag ataacacctt caatcaagtt aaaaggtaat accatggtca
     4561 ttaggtagtt ggaaagtccc aaaatttttc caatatcaaa gttagcaaac ttagcgtaca
     4621 aaggaacagc ataaacatag ttgagaacca acatggccaa ggttaaacca atagttccag
     4681 ctagagagcc tagtaggaaa cgaagggttg tccgttcctt tttccaaatc aaagcaaata
     4741 cgatgacaaa aactcccaaa gctacgatat tcatcggcaa accaatgtaa gtattcactc
     4801 cttggctgtt aagaagcaac ttcaagagtg agcgaagcaa gagcactcct agagcagcag
     4861 gcaaatccat gaccaccaga cccacaagga ctggcaagat actaaattcg atcttgagga
     4921 aagatgccgc tggtaaaagc ggaaagtcaa agtacatcag cacaaatgag atggctgata
     4981 gaattgcaat ggtcgaaagt cgacgtgtgt ttgtcataac aggttcctcc aattttctat
     5041 aaaatcagaa gaagttggaa aggattcctc tatctattct cactttttat atcccaaaag
     5101 ttccctctta ctctattaaa gaaaaacaaa gcaagtggtt acaatccggc tataaatcta
     5161 tcaaaacaga caaagctatt ctttcgtctt ctcccatcca gactatactg tcggttgtgg
     5221 aatctcacca catcagcttg cgctcgcgga cttctttaaa gagaaagaaa tagagatttt
     5281 tcttttagac taggcgacaa gctgtaggca taacttctgg ttaggcaaag cttgtcaaca
     5341 aagtataaaa ggaaaagatc atttcctagt taaagtcacc gccggtcggg aatcacaccc
     5401 tgccctgaag actcttttat cataacaaaa aacgcttgca agcgcaagca ttttgttcat
     5461 tttcattttt tatttcaatt tatccacttc ataggtatgg acgagctcaa gacctgcaaa
     5521 gttctgctgg cgaagagcct catagacaat catgcagacg gtattagaca cattgagact
     5581 gcggacatgt tcatcattca tgggaatacg gagagccttc tcaggatgtt ctcgcataaa
     5641 atcttcaggt aagcccttgt cttcacgtcc aaagagaaaa taatggtcct cgtcagtcga
     5701 taaatccacc tcagaataga ctttttccgc gaatttcgaa atcagataga gttttccctt
     5761 catctgagat atgaaatctt ccaaactctc gtaaaaataa atctctagct tatcccagta
     5821 atccaatcca gcccgcttca tcttgcggtc atcaatagga aagcccatag gcttgatgat
     5881 gtggagggga gaattggtcg cagcgcaagt acgcgcgata ttgcccgtat tttgtggaat
     5941 ttgaggttca aataatacaa tgtgatttgt catgacttgc ttcctttcac cattgcaaaa
     6001 aaatagccac actgcccgga gtcaagctca gcaaacagcg tggttaaggc atcgttaact
     6061 tacctcacaa caggtttgaa gtaaatcagc gaaactactt tcttagtata acactttcag
     6121 aatcattgtc aatagaaacg acttgatttt ttcaattttt tcaagctatt tccaagggtt
     6181 gtaaaatcgt ccctgattct gcaagataag tagtaaacta actactaaaa acaaggttgc
     6241 caagagcaag gtaagatagt ctcctttttt caaggcctga taactatacc atgtgcgttt
     6301 tttctctttc ccaaagcggc gaagctccat ggcagtcgca atggtatcaa tgcgttctag
     6361 cgagctaaaa atcaagggcg taataatgag cagattgcct ttgattcgtt gcataagaga
     6421 agctttcttg gataattcca tcccacgcgc ctcctgagac atcttgatag taaagaattc
     6481 ttcctgcaaa tctggaatat agcgcaaggt caggctgaca gaataagcaa tcttataggg
     6541 cacaccaatt tgatttaaac tggaagcaaa ctgactagga tgggttgtca tcaaaaagat
     6601 aatagccaga ggaatggtgc aaagatactt aatggccaaa tttagcagat aaaagagctc
     6661 ctggctggtt agagtgtaga caccgattcc ctgccaaatc acacttctct ctccataaag
     6721 tccaacccca tactcgggag aaaagagata gaccatcaaa acgtttaaaa cggcaaatac
     6781 cgtcgcaaaa acggctacaa aggaaacatc tttaaagcga atttctgata aatagaggag
     6841 aaagactgaa aagatggcaa tcagcaagag cattctggta tcatagctaa tcatggccgc
     6901 caatgatacc agaatgaaaa agagaagttt cccagctcct gacaagcgat gaatcacagt
     6961 atctctatgc tggtaaccga ttaatttagc ttgcatccct ctctcctttc tttgtaaaat
     7021 gccgttaaat ccaatggatc cacatctagt ttcttagcca agttaaagat ggaggtttct
     7081 tttagattag cttttactaa cagctcagga tcgctcaaca gactggctgg aacagtatcg
     7141 gcaatcaatt ctccatccac catgacaagg acccggtctg aataatccag catcaattgc
     7201 atatcatggg taatcatgac aatggtatgc cctttttgat gtaactcttc gagaaattcc
     7261 ataatctcag tatagttctt ctgatcttga cctgcagtcg gttcatctag gagaataatt
     7321 tcagctccta agaccaaaat tgaagcaatg gtgacgcgtt ttttctgacc aaatgacagg
     7381 gcagaaatag gccaattacg gaattcataa agtccacaga ttttcaaggt ttcatatact
     7441 ctcgtttcaa tttccttctc atccacacct cgcaaacgga gccctagagc cacctcatca
     7501 aaaatcatat tggttgaaat catttgatta ggattttgta gcacataacc tactcgttcc
     7561 gcccgctctg caacagaatc gccttttata tcctgttttt cccaaagata gcgtccttcc
     7621 gtctgaataa agctacttat agccttggct agagttgatt tccctgctcc attttttccg
     7681 acaatagcaa tcttttcacc ctttttaata tctaaatgta gggattttaa aatcggtcta
     7741 tcattataag aaaaagatac ttcctctagt ataaagagtg actgcaatgc tggggtttct
     7801 tttaccagtt ccgtctgtaa ctggatttgc ccttttgaga tagacaagtt atccagatct
     7861 gctaattgtt cttccttgac taagtccaca cctaattgac ggagagtcgt tagataaagg
     7921 ggttctcgaa ttccattttg agtcaataaa tcagtcgcaa gcaactggtc agggctccca
     7981 ttaaaaagga tacgaccatc gtttatcaag acaatccgat ccacagggcg atgcagaacg
     8041 tcctccaaac ggtgctcgat aataagagtc gtcgtcccct cttccttatg aatctggtca
     8101 atcaattcga taatatcctg acctgacttg ggatctagat tggcgagtgg ctcatcaaac
     8161 aagagaatcg gactttcatc aatcaagaca ccagccagac tgactcgctg cttttgtcca
     8221 cctgacaaat cctgaggacg ctgatccagt aaaggaagaa ggtccagctt ttcagcccat
     8281 ttataaacac gacctttcat ctcatctagg gctgtcacat cattttccag agcaaacgcc
     8341 aaatcttctg ccacagacaa gccaataaac tgcccatctg tatcctgcaa aactgtgcta
     8401 accagatgag acttatcata gatgctcata tcaaaggctg tttgaccctt tatcaaaaat
     8461 tctccatatg tctgaccctt gtaaatattg ggaataatcc cattcaaaca ctgacccaag
     8521 gtagatttac ctgatccaga tggtccaaca attaagactt tctctccctt gtaaatggtc
     8581 aagtctatcc cttgcaaggt cggttcttgt tgtgtttcat accggaaaga gaaatccttc
     8641 cactcaatta tagcttcttt catcttactc tcttcattcg cttcttagac ttctatttta
     8701 tcataaatca agcccttctt gcagtctctt ctcttaaaat cttagcgcca aaaagattcc
     8761 tatcctagct tacttgccta actaatctat aaacatcgaa aaagactagt tgcccagcct
     8821 tccccatcat tttatactct tcgaaaatct cttcaaacca cgtcagcttc gccttgccgt
     8881 aggtatggtt actgacttcg tcagtttcat ctacaacctc aaaaccatgt tttgagctga
     8941 cttcgtcagt ttcatctaca acctcaaaac catgttttga gctgacttcg tcagttctat
     9001 ccacaatttc aaaacactgt tttgagcaac ctgcggctaa cttcctagtt tgctctttga
     9061 ttttcattga gtattagtcc tttttcaaac ttcctgcacg agtttgggtt cctgcatagg
     9121 caagtaagag aagagttcct gcaatagcta cagatacacc attggcaatt cccgcaacaa
     9181 tcccttgtgc aaatactttt tctgccgctt cttgataaat cacaacatct ccaagtggtg
     9241 ccaagacacc ccaaacaagg gcatttgcaa gtagttgaat gaggttaaaa ataagaatat
     9301 ctttccagtc aaaaacacca ttgatcacgc gaacgtactt tctaaaaagt cccacaacta
     9361 aaccaaagag tccgctagcg ataatccaag tccaccatag accataacca gcaagagagt
     9421 ccttgattgc atgaccaatc aacccgacaa gcaaaccgat aatcggtcca aaaataatag
     9481 aaagtagcgc ttgtaccgca tactgaagct ggatgcttgt atttggaaca ggggttggaa
     9541 tgttgatcat cccgatgacg acaaagaggg cagcgccaat tccgacagca acaacttgtt
     9601 taattgtaaa tttgatttcc atactattct cctattttat ccttctattt tctttatttc
     9661 aatggtccaa gatgaaccga cacctacatt ataggccttg gcaaaggaac cttggttgat
     9721 agccaaacct aaacgataga gagagttgat gtaaaggatg ggttgcccaa ttctcacatc
     9781 tgcaaatgat ttgccataga caacctgatt ttgatagacc agcatatcag catgatagat
     9841 ggtcacttca aaacgatcac caaattctgg ttccaacttg taaaattctt cccgtgtgat
     9901 agaggtccaa agcgaaccga aacgcacatc cagaatatca atggctccct tcaccagatg
     9961 gtcttctatg atggtcgcta cgactggaag ctctacaatc tgttccacac tgagctctgg
    10021 ccctacttcc tcaaaagtaa tgtgaccact ggccagttta gcaccagtat aggcatagac
    10081 atcacgaccg tggaaggtat aagaatgctc tgtgttttga cgcctattgg ccacctcaga
    10141 aatctcacga atggctacaa tgccaacgtg tttcttgata aaggaaagcg tcccattatc
    10201 tggcgtgaca atgtattgat tttttgcagt cttggcaact acactcttac gtttcgaacc
    10261 gacacctgga tcgacaaccg atacaaacgt cgttccctca ggccagtaat ccaccgtctg
    10321 aaagagacga tagctcccct caaaaatatt ataaggcgtg atatcgtgcg tcaagtgatg
    10381 gatttttaag gttggagact cttctaaagc cactccaatc atagccgata ccgcaccatc
    10441 aaccagacca aagtctgatt gtaataccag taaattatta ttcattttat ctccttgtat
    10501 atcctttatc ataccatatt tcagagaaag tcgctaatag ctatttcaat tggttagggt
    10561 atagttttaa cttatagcaa atttcctact aaaaactctg gttattagct agtaggtgca
    10621 ttttttcttg aaaaagggta tgatagttac atcatgaaaa agtaggtttt attatgaaaa
    10681 ttatccttgt cggaggggga aaagttggtt ttgccctctg tcgctccttg gttgcagaaa
    10741 agcatgatgt tttgctgatt gagcaagacg aagctgttct caatcatatt gtcagtcgct
    10801 ttgatatcat tggtatcctt ggtaacgggg ccgattttgc cattcttgag caagccagcg
    10861 tccaagattg tgatatcttt atcgccctga ctgagcacga tgaagtcaac atgattgcag
    10921 cagttctagc caagaaaatg ggagctaaag aaactatcgt tcgggtgcgg aaccctgaat
    10981 attctaactc ttatttcaag gaaaagaata ttctcggttt ttctcttatc gttaatcctg
    11041 agctcttggc tgcccgcgct atcgcgaata tcattgactt ccccaacgcc ctgtctgtcg
    11101 aacgctttgc tggtggacgc gttagcctca tggaatttgt cgtcaagtcc accagcggtc
    11161 tttgccaaat gcccatttct gattttcgta aaaaatttgg taatgtcatt gtctgtgcga
    11221 tagagaggga tcatcaaatt atcattccaa gcggtgacat gactgtacag gataaagata
    11281 gaatctttgt cactggtaac cgtgtcgata tgatactctt ccataattat tttaaatcac
    11341 gcgccgtgaa gagccttctc atcgttgggg caggtagaat tgcctattat ctacttggta
    11401 ttctcaaaga tagtcgtatc gatacaaaag tcattgaaat caatcctgaa atcgccagct
    11461 tctttagcga gaaattccca aatctctaca tcgttcaagg agatggaacc gcaaaagata
    11521 tcctgctgga agaaagtgct caacactatg atgccgttgc gactctaaca ggagtcgatg
    11581 aggaaaatct gattacatct atgttccttg acagggtagg tgtacagaaa aatattacta
    11641 aggtcaatcg taccagtctc ctcgagatta tcaatgcgcc tgatttttca agtatcatca
    11701 cacctaaaag catcgctgta gatacgatta tgcactttat tcgtggtcga gttaatgccc
    11761 agtattcaga ccttcaagcc atgcaccatc tagccaatgg ccaaattgaa accctgcaat
    11821 tccatatcaa ggaagccaat aaaatgactg ccaaacctct ttctcaactg aaattgaaaa
    11881 aaggggttct tattgcagcc attattcgaa agggcaagac tattttccca actggggagg
    11941 atatgttgga agttggagac aagctcctag taacaacctt gttgccaaac atcaccaaga
    12001 tttatgactt gattgcgagg taagaaatga ataaaagtat gattcgttac ctcctttcaa
    12061 agttactttt gattgaagct gttctcctct tggttcctgt ggctatcgct gtctattacc
    12121 gtgaatcgag ccaagtcttt acagccctct tttcgaccat agggattctc gtattgttag
    12181 gcggttcagg gagtttacag aagccaaaaa atcaacggat ttatgccaag gagggagtct
    12241 tgatcgttgc cctctgttgg atcctttggt ctttctttgg tggtctcccc tttgtctttt
    12301 ctgggcaaat ccccagcctt attgatgcct tttttgaaat tagttctggg tttacaacta
    12361 ctggagcaag tattttgaac gacatttcgg ttctcagccg ttccctcctc ttctggcgaa
    12421 gttttaccca cttgattgga gggatgggag tgcttgtttt tgcacttgct attatggaca
    12481 atgccaaaaa tagccaccta gaggtgatga aggctgaggt tccaggtcct gtttttggca
    12541 aggttgtatc caaactaaaa aacactgccc agattctcta tctcctttat ctagctctct
    12601 tctccctctt tgtcatcatc tattatctag ctggtatgcc tctctacgat agttttgtca
    12661 ttgctatggg gacagcggga actggaggct ttaccgtcta taacgacgga attgcccact
    12721 atggcagctc actgattacc tatctggtca gtatcggagt tctggttttt ggggtaaatt
    12781 tcaacctcta ctactacctc atgctccgtc gcatcaaggc cttctttgga gatgaagagc
    12841 ttagggctta cttggtcatt gtactggttt ctacaggctt gattagcctc aacaccctct
    12901 acctctaccc agggttttca aagagctttg aaatgacctt cttccaggtt tccaacatca
    12961 ttacaacaac tggttttgga tatggagata ttaccaactg gcccctcttc tcccaattta
    13021 tcctcctttt cctcatggca atcggtggct ctgctggatc aactgcaggt ggactcaaga
    13081 ttattcgagg cctcatcctt tcaaaaattg ccaaaaacca aattttgtca atcctatcgc
    13141 cccaccgtgt tttgaccctc catgtgaatc aaacggtgat tgataaggat acccagcata
    13201 agattctcaa gtactttgtc atctatgcta tgattttgct agcgcttatc tttattgtca
    13261 gcctagatag caatgatttt ctggtcgtga ccagcgctgt ctttagctgt ttcaataata
    13321 tcgggcctat tctaggaacc acttctagtt tctcaatctt tagtcccatc tcaaaaattc
    13381 tcctatcctt tgcaatgatt gcagggcgct tggagattta cccaatcctc cttctcttta
    13441 tgaagagaac ttggtctaag agataaaata caaaatccct ctttttacaa ttgtaaaaga
    13501 gggattctta tattaaaaaa gggggaacgt ttttaaatcc aagcctatct gtaagcaaag
    13561 tccttactca aataaaagat ggaaaaactt actacacagc ttggtaatgc tccaatacaa
    13621 attgtaagga tttacttgga ctctttgtta aatgaatata ttccattcct ctggtagcaa
    13681 ctatctgaat acccaatctt tctgcatcca ccttcatctc tctataataa ctccgaactt
    13741 gtggggccag aataattaaa tcataaacac ccataatatc ataatgagct ccgtacgctc
    13801 ctgaattagc aatcactcta acctctgtta agttagcccc ctcgttaatt gcattggcta
    13861 attgcgcact tgttccagac cctgcacaaa gaaccagtac tttcaactcc ttacttttta
    13921 ttacatctat ctcaccaggc attatttcac ttgtatcctc ctctaaaata atatcatttg
    13981 agcttgcaat atctacaacc tgtagtaaat atctcgtatt tagacaatat gaaaacaaga
    14041 cgacttccat ataggaaacc gccttctcgc tatgttgagt gatttatatt aaaataactt
    14101 ttcttctagc tgcattttat tattataaaa acattcatca taaccctgaa cttaaataac
    14161 gatttttatt caagatacat actcctagaa taaactttat atgaaattct catttttgtt
    14221 tttacaattc tccttagtta aatcttgttt aatatatgtt ttacatatag tatttagcgc
    14281 cacatagtac tgaactctct ccaaaaacgg ttattcctct ttgaataggg cgttatcaca
    14341 agaaaagcat ctccacgttt caacttcata tggctcaaaa acaatcaatt gatgctaaaa
    14401 cctgtaccta gatgtttcgg ttcataaaac catgaaactg taaaagtgga tgaaattgat
    14461 agcgatagtc aaatcaagag gcatcataac tctaaaaagt cacaatatat aagttcatcc
    14521 tcggaaaaat atcattctaa ttgttgaaat gcctacatga aaagaaacgt caaatgctca
    14581 tgaaacaacg aatacaggta tcaaaactat gacaaaacaa gtccctaaat ttactaaaga
    14641 cactgctcaa ctttacacct gtaaatggtt gttgtataat aaagttacaa agatgtacga
    14701 ccacactgtt gtaaatcata gtgttcgcga atatattact gatagcattt ctacaaatac
    14761 aattgaagag actggataga ctttaaacga aatatgtcag tgttttggca ttcctagcct
    14821 tcatatcatt taaagaattc tatagacaaa atttttccca atacagacac tcgtaacaac
    14881 tgcttcattt ttctaccaac atatttagga acaggataag atacaagagg attaatccat
    14941 agatcagttc tataccaatc taagacaaat aagctaaaaa aacgattgat aataagcaaa
    15001 tagattccaa attttctcta tctgctcatt ttaataaaca atactagtgt aactatcctt
    15061 ccagtcagaa gcttgtcaaa tcacaccgaa aattcttcta aaatttatct cgttaggcaa
    15121 tcaagcaaaa actcgacgat agtacaaaca ttatcataca g
//
LOCUS       DAVLRT010000043        14986 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLRT010000043 DAVLRT010000000
VERSION     DAVLRT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.043"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..1008
                     /locus_tag="VXM85_001347"
     CDS             <1..1008
                     /locus_tag="VXM85_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1345917.1"
                     /translation="DFQLIEGLITHLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMT
                     REILANMDKFLEWTISDDEVAYVSLHFLAAMERSKESTKFNILAICATGFGAAQMLRN
                     RLETEFGKRVEVVDVIGYYELNQEKLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDE
                     MEMIRKAMDQMQVSSYVQSSKINKFENNSFRQYFSKENFLICTESDKVNLLEKMVESL
                     SVGESNEFEQSLLYGIKQREELSSVVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWD
                     NQSSYVQLVFLLSPSIYGNEGLATVTKKIVSLTENDELQNQLISCNNFEDFINIFEKI
                     K"
     gene            1018..1332
                     /locus_tag="VXM85_001348"
     CDS             1018..1332
                     /locus_tag="VXM85_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEV1345918.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            1363..1869
                     /locus_tag="VXM85_001349"
     CDS             1363..1869
                     /locus_tag="VXM85_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345919.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFENLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            1949..3295
                     /gene="celB"
                     /locus_tag="VXM85_001350"
     CDS             1949..3295
                     /gene="celB"
                     /locus_tag="VXM85_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEV1345920.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNSLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            3675..3851
                     /locus_tag="VXM85_001351"
     CDS             3675..3851
                     /locus_tag="VXM85_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345921.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALSLGMT"
     gene            4253..6466
                     /locus_tag="VXM85_001352"
     CDS             4253..6466
                     /locus_tag="VXM85_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1345922.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEDEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(6688..7164)
                     /locus_tag="VXM85_001353"
     CDS             complement(6688..7164)
                     /locus_tag="VXM85_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1345923.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTNPKQIEEAIQTLL"
     gene            7387..10590
                     /locus_tag="VXM85_001354"
     CDS             7387..10590
                     /locus_tag="VXM85_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1345924.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSTWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDIASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKSGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            10970..11355
                     /gene="tnpA"
                     /locus_tag="VXM85_001355"
                     /pseudo
     CDS             10970..11355
                     /gene="tnpA"
                     /locus_tag="VXM85_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(11734..12363)
                     /locus_tag="VXM85_001356"
     CDS             complement(11734..12363)
                     /locus_tag="VXM85_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1345925.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(12373..13374)
                     /locus_tag="VXM85_001357"
     CDS             complement(12373..13374)
                     /locus_tag="VXM85_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1345926.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(13405..14046)
                     /locus_tag="VXM85_001358"
     CDS             complement(13405..14046)
                     /locus_tag="VXM85_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1345927.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(14065..14880)
                     /locus_tag="VXM85_001359"
     CDS             complement(14065..14880)
                     /locus_tag="VXM85_001359"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1345928.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         4763 a         2482 c         3138 g         4603 t
ORIGIN      
        1 gatttccaac taattgaagg attgataact catctaatga cactacaagt acgtttagaa
       61 agccggatta ccttaaataa ccctttggta gatgaaatta aacagaatta tagtgatata
      121 ttctttatga ctagagaaat tcttgcaaat atggacaagt tcttggagtg gacaatatca
      181 gatgatgaag tagcatatgt aagtcttcac tttttagctg ctatggaacg tagcaaagag
      241 agtactaagt ttaatattct tgctatttgt gcaacaggat ttggtgcagc ccaaatgtta
      301 agaaatcgtt tggaaacgga gtttgggaag cgagtagaag ttgttgatgt aataggctat
      361 tatgaattga atcaagaaaa acttaaagga atagatttta tagtgtcagc tgttgattta
      421 tcaaatctat attttcaaat tccagttttt aaagttagtg tgtttcttaa gagtgatgag
      481 atggaaatga ttagaaaagc tatggatcag atgcaagttt ctagttatgt acaatcatca
      541 aagattaata aatttgaaaa caacagcttt agacagtatt ttagtaaaga aaatttttta
      601 atatgtactg aatcagacaa ggtaaattta cttgaaaaga tggttgagag tttatctgtt
      661 ggtgaatcaa atgagtttga acaatcttta ctttatggaa ttaaacagcg cgaagaatta
      721 agttcagttg ttttttctga aaaaattgca gttccgcatc ctattcagcc ctttggtaca
      781 gagggaaagg tttcggttgc aatttgtaaa gattcgttac tctgggataa tcaaagttca
      841 tatgtccagc tagtatttct tctatctcca tcaatatatg ggaacgaagg tttggcaact
      901 gtgactaaaa agatagtctc tctgacagag aatgatgaat tgcaaaatca gctaatatct
      961 tgcaataact ttgaagattt tattaacata tttgagaaga taaaatagga ggttcatatg
     1021 aatcaagagg aactgcaggt tgcagcattt gaaattattc tgcattcggg aaatgcaagg
     1081 tcagaaatac atgaagcatt tgcaaaaatg agagaaggta gttttgatga tgcagaaagt
     1141 aaattaaatc aatcaaatga gataattctt gaagcgcatc atgcacaaac caaactcttg
     1201 caagaatacg caagtggagt ggaaattaaa attgaaatta tcatggtaca tgctcaagac
     1261 catttaatga ccactatgac attattagaa gtagcaaagg aaatgttagc tctttataag
     1321 aaagttaact aaatatgtgt tgttatataa ggaggaaatg atatggataa aaaagaacga
     1381 caaaaaattg aacaacaacg tcgagagatg gctctaacta atactttttt taatcgatat
     1441 cttcttctac gttattcaat agcactcttc ttttttggga atatttattg gcttttgaat
     1501 cagtttatta gtccctcgcc tattattatt ttccccatta tgctcatagt attttctatt
     1561 ttagcaacta ttgaacaatt taaactttat ggaaatagga aagaaaagtt gggaataaca
     1621 ctaatgtttg ttagaataca aatgcttatt tcaataggat tacttgtttt aacttggaca
     1681 agttggtttg agaatctttt tccgattttt gaaaataacc aagtagcacg tttatttgtt
     1741 tttgtagttc ttctattagg tttagttctg agtttgttag acattagaag aattaaaaaa
     1801 atttataaac gaacagataa ggcttatcaa caatttgtcc aattggaaaa gaactcactt
     1861 agtttataat cctgaactag tataatttgt atttgaaact agtctttaaa aagtaaaaat
     1921 tattatgttt tattaaggag gtctatttat ggatcaacaa aacgggttgt ttggttttct
     1981 tgaaaaccat gttatgggac caatgggcaa acttgctcag tttaaagtag tgcgtgctat
     2041 cacggctgca ggtatggctg ctgtaccatt tactattgta ggatcaatgt ttttggtatt
     2101 cagtattttg ccacaagctt tctcattttg gccaattgtg gcagatattt tctctgcttc
     2161 atttgataaa ttcacatcac tttacatggt tgcaaactat gcgactatgg gttctctatc
     2221 tctttatttc gttctatcac ttgcatatga attgacaaaa atttatgcag aggaggaaga
     2281 actcaatatg aattctctta atggtgcctt gctcgccttg atggcttttg tcatgacagt
     2341 accgcaaatc atttttgatg gtggaatgat gaagactgtg acaagtctaa aagaaggtgc
     2401 agtaattgca gatggatggg caatgggaaa tggagtcgca cgttttggta caacaggaat
     2461 ttttaccgca atcattatgg caattgtgac tgttcttatt tatcgtatgt gtgttaaaca
     2521 taattgggtt attaaaatgc ctgaagctgt tccagaagga gtttctcgtg gatttaccgc
     2581 tttggttccg ggatttgttg ttgcatttgt tgttatcttt atcaacggtc ttcttgtagc
     2641 aatgggaaca gatattttta aagtcattgc aattccattt ggttttgtat ccaatctgac
     2701 taattcgtgg attggtttaa tgattattta tctattgact caactacttt ggattgtagg
     2761 tatccacggt gcgaacattg tttttgcatt tgttagtcca attgctcttg ctaacatggc
     2821 tgaaaatgct gctggcgggc acttcgctgt tgcaggtgaa ttttctaata tgtttgtaat
     2881 tgcaggtggt tctggtgcaa ctttaggact atgtttatat attgcttttg cctctaaatc
     2941 tgaacagctt aaagcaatag gacgagcatc tgtagttcca gccttattta atattaatga
     3001 accattaatt tttggattac ctattatcta taatccagcc ttggctatac catttatttt
     3061 agcaccaatg gttactgcta ctatttatta cgtagcgaat tctctaaact ttattaagcc
     3121 aattatcgca caggttccat ggccaactcc agtagggatt ggagctttct tagggacagc
     3181 agatcttcga gctgtattag ttgctctagt atgtgcattt gcagcattcc tagtctatct
     3241 tccattcatc cgtgtatatg atcaaaaatt ggtgaaagaa gagcaaggta tctaataagt
     3301 aaaaaagtat actcgatgat agaatccata tagtttaaat aatgagcgtt aacattagca
     3361 gtgaagaggt ctttttaaga tattcttctc tgctttttca tattttttcg ctatgtagag
     3421 tatgaagcaa aatagaaagt agttcgaata gttgataaac agggaattgc tgttatttaa
     3481 aaaaaacaat aaagtaaagt atgttatcgt agaatacgat acaatagtaa aaaagcaaaa
     3541 agaatgcgtt ttatatttgg gtttgaggct tataaaatag gataagcgta ctgaaaactt
     3601 agatatgaca aacaaaataa aatttttaaa agctacagag aaacatggta taatgtatct
     3661 aaaggaggta gcatatgtcc ttagatatag ataaagaaaa gatgacaatc atgggaatag
     3721 cttttgaaaa ccgatccgtt tttaagagtg tttggtatgc tttaagtaca aatatgattg
     3781 aaggatggcg acctacggtt agtgatgttg aaaaattacg agatgaagct ctttctctgg
     3841 ggatgactta atgaagcgca actgttatga gtcttatgac tatgttgatc cggataattt
     3901 atataccgat ccgggttctt cagttctaag aaataagcag gaaggaaaaa agcacgcgag
     3961 cttgaatatc gtatgattgc tagtaagagt ttgaaattat ttatcaatcc tatcttagtt
     4021 aatagtttaa aagtagtttg tttcacaact gcataagctt agaaaagttg aaaagtgata
     4081 actctttaat aaactcttgg tttgttaaaa gatattttgt ccactacatt tttttcaatt
     4141 gactattatt tgtcatagat ataacagata gaacattgca ttcagaataa tgaaaaacgc
     4201 atcaaaaatg gtgcgttctg tttttatgct accataaaag gaggaaaaca aattgaaaat
     4261 ttttaaggga gagttttatc gaatctctgt attaacagac aagctagtaa ggttagaata
     4321 ctctcgaact ggaaattttg aggatagaac gacacaactt atctataata gagattttgg
     4381 ccaagtttcg ttagattata tcgagacatc aaatgtacta gatattatga cggactattt
     4441 tcatctgcac tttaataaag gagaatttaa cgccgaaaat ttatttatag aattaaaagg
     4501 aaattttgcc gtatatggta gtcgctggta ttttggtgaa tctattgaaa cgttaaaagg
     4561 aacagctcgg actctggata aggcagatgg agcaatctcg ttagaagatg gaattattag
     4621 ccgaaatggt atagccttat tggatgattc tcaaggattt atttgggatg aacaatctgg
     4681 ttatattgag agagaaaatc aaattgacct gtatttcttt gcctatgggc atgattatag
     4741 aggagcaatc agagactttt accatttgac tggttcaaca cccttgttgc caagatatgc
     4801 tttaggcaat tggtggagta gatattggcc ttatacgtcg gatgaatact tggatttaat
     4861 agacagattt gaaacagaga aaattccatt atctatcggt gtgttagata tggattggca
     4921 tataactgac attccagctc gttttggaag tggctggaca ggatatagtt ggaatagaaa
     4981 cttaatacca aatccagaac agttattgca acaacttcat gatagaaagt taaaactctc
     5041 cttaaatgtc catcctgctg atgggatacg ggcttatgaa gaagcttatc ctcaaatcgc
     5101 aaaacggttg gggttaaatg tagaactaga agaacctgct atttttgatt tttttaatcc
     5161 ctcttttagg gaagcctact ttaaagatgt tcattatgag ctagaaaagc agggagtaga
     5221 tttttggtgg attgactggc aacaagggac acaaggtatg ctagatccac tttggctttt
     5281 aaaccattat cactatcagg atagttgtaa aaatgcagaa ggtggtttga ttttatcaag
     5341 atatgcaggt cctggtagtc accgctaccc tgttggtttt tcaggggaca ctattattag
     5401 ttggaattcc ttaagatttc aaccctattt tacagcgaca gcatctaata tcggttatag
     5461 ttggtggagt catgatatcg gtggacatat gttgggggat tatgacgaag agctacaaac
     5521 tagatggcta cagtttggtg tttttagtcc gattactcga ttacatagtt ctagaagtcc
     5581 ttttaatagt aaagaacctt ggttcttttc agaaacaaca tctaagatta tgaagaaata
     5641 ccttcgtttg agacatcaga tgattcccta tctatatacc atgaatgtaa agacacatga
     5701 ggaaggtgcc ccattaatca gtccaatcta ttatttctat ccagagaatg atgagagcta
     5761 taatgttcca aaccaatact tttttggaac agaactgatg gtggctccca ttgtagaaaa
     5821 gatggatttg acattccaat ctgcaaaagt agatgtatgg ttccctgaag atgaatggta
     5881 tgacttcttt tcagagaaaa aatacacagg tggtgtgaag ttaagtgttt atagggacat
     5941 ctcgactacg cctgtgtttg caaaaagtgg tgcaatcatt cccttggttg gttctgagat
     6001 aggtatgggt gttgatttac ctgaggttgt agattggtat gtattcccag ggaaacaaca
     6061 ttcttttgaa atgcttgaag atcaaaatgg tcaaagatat aaaacaagat tatcaatcga
     6121 ttgggaaatg ggaatggtag agttagcatt acaaggagat tctagtatcg ttccaagcaa
     6181 tagaaaacat agaattcatt ttaaaggaac gaatgtgtct ataattgaat tgccaaataa
     6241 gaatgataca gctaaatttg aatggaaaga taataaaagg acatctttaa atgacgaagt
     6301 ttttagacta ctaaagacag cttctcttcc atatgaatta aaagatagat tgttaaatca
     6361 attcatcaat gcgaaaaact ctcatgactt aatgaatatc ttgcatcatc aggataagga
     6421 attgagaggg cgtttgttgg aaatgatatt tactagccaa aactaattga aataattaga
     6481 aagtcatttg tttagtgtat atgaagattc tgtatggtat tcgttatcac tggtcacatt
     6541 gaaaatgtga aataagcacc tacaacatag actttacaac acttcatgtt ttatatcatg
     6601 gggtgttttt ctatttaagt caaagcccat caaaaaatct cactattcat caggttaaag
     6661 gaaacctaat catagtgaga ttgtgaatta tagtagagtt tgtatcgcct cttcaatttg
     6721 ttttgggttt gtttttgaag aaaagcgttc aaagacttgc ccatcacgac caatgagaaa
     6781 cttagcgaaa ttccattcga ttcgttttcc tagtgggcca gatttatggt cttttaacca
     6841 gacatagaga gggtctgctt ccttaccgtt gaccttaatc ttggcaaaac gtgggaaggt
     6901 ggtttgaaaa tgtaggctac agaaggcgtt gatttcctct gcgctgccgg gtgcttgtcc
     6961 cataaactga ttgcaaggga aatccaatat ttcaaagccc tgatcttgat agcgttcata
     7021 gagttcttga agtccctggt actggggcgt taaaccacat ccagtagcag tgttgacaat
     7081 caagagaact ttaccacgat agctatccaa gggagttgct tggttgtttt ggttcaaaac
     7141 ggaaaaatca taaagtgaag tcatgagggc ctcttttctt tttctacatt gtaacagttt
     7201 ttactttttt cgtcgagtcg gatggaagat attgttgaag aataagcctt caatatgata
     7261 gaaaccgttt acaaataact ttaaaaatgg tatgatttaa ttaaaccggt ttactttgtt
     7321 gctataaatc tctaatttga gatttagttc ctattgattt tacaatatgt ttattggagt
     7381 gtatacatgc aaacaaaaac aaagaagctc attgtgagtt tgtcttcact tgttttatca
     7441 ggatttttat taaaccatta tatgacagtt ggagcggaag aaacgactac gaataccatt
     7501 cagcaaagcc agaaggaagt tcagtatcag caaagggata caaaaaattt agttgaaaat
     7561 ggtgattttg gtcagacgga ggacggaagc agtccgtgga caggaagcaa agctcagggg
     7621 tggtcaactt gggtagacca gaagaatagt tctgcagatg cctcaactcg agtcattgag
     7681 gctaaggatg gggctatcac tatctcaagc catgagaaat taagggcagc gcttcaccgt
     7741 atggttccta ttgaagctaa gaaaaagtat aaactgcgtt tcaagattaa aacagatcat
     7801 aaagtcggga ttgccaaagt tcgtatcatt gaggaaagtg gtaaggacaa gcgattgtgg
     7861 aattctgcaa cgacgtcagg aacaaaggac tggcagacca ttgaagcaga ctatagcccg
     7921 actttagatg ttgataaaat caagctggag ttattctatg aaacaggaac tgggactgtt
     7981 tcctttaagg atattgagct ggtagaggta gcagaccagc cttctgagga ttctcaaaca
     8041 gataaacagc ttgaggaaaa gattgattta ccaattggaa aaaaacatgt tttttctctt
     8101 gcggactata cttataaggt agaaaatcct gacattgctt cagtcaaaaa tggaatttta
     8161 gaacctctta aggaagggac aaccaatgtc attgtcagta aagatggcaa ggaagtgaaa
     8221 aagattcctt tgaagattct agcctctgtt aaggatgcat acacagaccg tttggatgac
     8281 tggaatggca tcatcgctgg gaatcaatac tatgattcta aaaatgaaca gatggccaaa
     8341 ttaaaccagg aattggaagg aaaggtagct gatagcctat ctagtatttc aagtcaggcg
     8401 gaccgcacct atttgtggga aaaattttca aattataaaa tgtctgcaaa tctgactgcc
     8461 acttatcgga aattggagga gatggccaag caagtgacca atccttcttc tcgttattat
     8521 caagatgaaa ctgtcgttcg aacagtcagg gattccatgg aatggatgca taaacatgtc
     8581 tacaatagtg aaaagagcat tgttgggaac tggtgggatt atgaaatcgg tacacctcgt
     8641 gccatcaaca ataccttgtc tctgatgaaa gaatacttct ctgatgagga aattaaaaaa
     8701 tatacagatg tgattgaaaa atttgtacca gatcccgaac atttccgaaa gacgactgat
     8761 aacccattca aggctctagg tggaaactta gttgatatgg gaagggtaaa agtaatagct
     8821 ggtttactgc gtaaggatga tcaagaaatt tcttctacca ttcgctcgat tgagcaagtg
     8881 ttcaagttgg tagaccaagg tgaaggtttt tatcaagatg gatcctatat cgaccacacc
     8941 aatgttgcct atacgggtgc ttatgggaat gttttgattg atggcctgtc tcaactgttg
     9001 ccagtcattc aaaagaccaa gaatccaatc gataaagata aaatgcaaac catgtaccac
     9061 tggattgata aatcgtttgc tcctttgctg gtgaatggag agctgatgga tatgagtcgt
     9121 ggacgctcga tcagtcgtgc aaatagcgag gggcacgtgg ccgcagtaga agtactaaga
     9181 gggattcacc gaatagcgga tatgtctgaa ggagaaacca aacaacgttt gcagagtctt
     9241 gtgaagacca ttgttcaatc ggatagttat tatgatgtct ttaagaattt gaagacttat
     9301 aaggatatca gtttgatgca atccttgtta agtgatgcag gagtcgcaag tgttccaaga
     9361 acaagttacc tatctgcctt taacaagatg gataaaacag ccatgtacaa tgcagagaaa
     9421 gggtttggat ttggcttgtc actcttttcc agccgtacct tgaattacga acacatgaac
     9481 aaggaaaata aaagtggttg gtatacgagt gatgggatgt tctatcttta caatggcgat
     9541 ttgagtcact atagcgatgg ctactggcca acagttaatc catataagat gcctggtaca
     9601 acagagacgg atgctaagag agcggatagc gatacaggta aagttttacc gtctgctttc
     9661 gttggaacga gcaaactaga tgatgccaat gcgacagcaa ccatggattt caccaactgg
     9721 aatcaaacat tgactgctca taagagctgg tttatgctaa aggataagat tgccttttta
     9781 ggaagcaata tccaaaacac ttcaacagat actgctgcaa ctacaattga ccagagaaaa
     9841 ctggaatcaa gtaatccata taaagtctat gtcaatgata aagaagcctc ccttacagaa
     9901 caagaaaagg attatcctga aacccaaagt gtctttttag aatcgtccga ttcgaaaaag
     9961 aatattggtt actttttctt taagaagagt tcaatcagta tgagtaaggc tttgcaaaag
    10021 ggagcctgga aggatatcaa tgaaggacag tcagacaagg aagttgaaaa tgaatttctt
    10081 actattagtc aggctcataa gcaaaatgga gattcttatg gctatatgct cattcctaac
    10141 gtggatcgtg ccaccttcaa tcaaatgata aaagagttag aaagtagcct catcgaaaat
    10201 aacgaaaccc ttcagtctgt ttatgatgct aaacaaggag tttggggcat tgtgaaatat
    10261 gatgattctg tctctactat ttccaaccaa ttccaagttt tgaaacgtgg agtctatacc
    10321 attcgaaaag aaggggatga atataagatt gcctactata atcctgaaac ccaggaatca
    10381 gctccagatc aggaagtctt taaaaagcta gagcaagcag ctcagccaca agtacagaat
    10441 tcaaaagaaa aggaaaaatc tgaagaagaa aagaaccatt cggatcaaaa gaatctccct
    10501 cagacaggag aaggtcagtc aatcttggca agtctagggt tcttgctact tggggcgttt
    10561 tatttattcc gtagaggaaa gaacaactaa tttgttcatc atctagatga attacatgaa
    10621 actgtggaaa atagaaaaaa tccgaagaag gccaaatatc ttcggatttt tcttttaaaa
    10681 agggataagt tgatacttat ccttttttac ttcattatgt gactatagcg ttttgaaact
    10741 agaataggac gtcataactg ctaaaagatt tctataaatt catttgattt tcctaatcaa
    10801 tttgttcgta tcctatttca ctccactata ttagtccgtc tcgctccgtg aggtgcgaga
    10861 caaaacaacc acccgctatg cgggtgcgcg tcgaaggcta taccaaaaaa ctccaaacgc
    10921 gatacaataa aggtgttcaa tccaattgta aagcgaaagg agaaaaaata tggcacaaaa
    10981 gactcatagt ttatcataca caaagtggat gtgttctatc atattgtgtt cacccctaag
    11041 tatagacgaa aagtcatcta taatcaatat agaagtagtt taggcgaaat atttcatcgc
    11101 ttgtgtggtt ataaaggtgt tgaaatgatc gagggtcact taatgccaga ccatgtacac
    11161 atgttagtca gtattccact aaggctaagt gtttcgagtt tcatggggta tttaaaaggc
    11221 aaaagtgcac tcatgatgtt tgacaaacac gccaatctca agtacaagtt tgggaatcgg
    11281 catttctggg cggaaggtta ttatgtaagt acagtagggc ttaataaagc cacacttaag
    11341 aaatatagtc aagattaaag aaattatcga gtggatgatt tcttcacgag tatgaaaatt
    11401 tgagaacgag taaagtatga tatatagtaa aatgaaataa gaacaggaca aaatcgatca
    11461 ggacagtcaa atcgattttt aacaatgttt tagaagtaga ggtgtactat tccagtttca
    11521 atctactata gcactagata aattgagtgt aaaagaatat gaggatccct ttagggatag
    11581 tggtaagtaa taccaaagtc tcttaaagag gcaagtgacg agtcaagagc aataaggctt
    11641 gaacaacgtg aaagccagcg tctttaggcg ctggctggta atttgggctt atagctctgg
    11701 gataaaccac ccgtttgacg ggtggttatg attttatctg agtgtaacat actgttgggc
    11761 aatctcgctg atgcggtcaa agttgccttg ggaagcgagt ttattgagtt cgccaccaat
    11821 tccaacggcg tctgcaccag cagcgaacca ttgagggatg ttgtttagac cgactcctcc
    11881 ggttaccatt acggaaactt gtgggatcgg tgccttgact gcagagatat atgctggact
    11941 gagagcacta cctgggaaga gtttgatgat ttcactaccg gcttcaagtg cagtcgtgat
    12001 ctctgtgagg gtaatacagc ctggaatgta cggtgtgctg tagagattgc acattttcgc
    12061 agtttcagca tggaaagatg gagaaacaac gtaatttgct ccggctagaa tggcatctct
    12121 agcagttacg gcatcaagca cagtacctgc accgatacaa acactctgat cgtcctgata
    12181 caagtctaca agttccttga tgatttgtcc tgcatactga ttggtatagg cgatttcaat
    12241 agctttgata ccgcccttga tacaagcaat cgaggcttgc agtccttctt cctttgtatt
    12301 tcctcgaatg acagcgacaa ttttctgttt ttttagttca ataatcgtat ctgatttggt
    12361 catgtaattc tcctaacgaa tgatatcttg tgcatttgcc agtaaatttt caatactagt
    12421 tgcggaagtg gagagatggt ctcctggaag agtgcatttg agagttgcgc tcgcaactgc
    12481 aaagtcaatg gtagttttta gggaggaatg atggagtagt tggtaaaggg caccagatat
    12541 aaaggcatcc ccgctaccaa ttcgttgata gactgcagtt tttagttgga cagactcttc
    12601 aaatcgttct tccagagcat aggcttgata gacatttttg tcttgctcat cactagagcg
    12661 gagggtatgg aaaatggcct tgaaaccata ggcttctttt aaaagtcgca tgcgattttc
    12721 cacctcttct aggctagcac tgtctcttgg aaacatctct agattttggt catcaatcat
    12781 gagaggatca ataccgaagc aatagtcagt aaaacgtgca aacttagaaa attcatactt
    12841 ggcttctagg actgaaatca tctttgttct cagattgaga tccattgaaa cgacaattcc
    12901 tcggcgcttg gcttcttcca agagtaggag aaggatcgca cggacctctt gaccgatagc
    12961 tacggtgatt ccactaaaat gaaaatggct aatcccctga aagagagaat ccatatctag
    13021 catgtttggc cgaatctggc tgatactcgt atgcttacga tcgtagaaaa cttcactttg
    13081 acgacaacca aagccgttct ccaaatagta gaggccgatt cgatcgccaa gccgacagat
    13141 tgaactggta tcgatttggt gctgtttcaa gaatgtgaga aaacgatctc caatctcgtt
    13201 ggcagggagt gcggtaaaga ctttcgttga gatacccagg gcttgcaaat tacaagcgat
    13261 gttaatttct gatccgccaa aataagtcga actggcaaca tgatcgccga tactggtggc
    13321 atctaatggt gagattcgaa ttaatggttc gccaaacagt aatattttcg tcatccatta
    13381 gttcctttct ggtttagaat agctttactt ggctaatact tctttcaaat aagcagcgac
    13441 ttcatcatct tggcagtttt caaagaagta ttcttggaaa tatttgccag agatggtgtc
    13501 tttgaggaag tcttggtcga ttgttttaag gatggtcatc aaatcattgt gggtgatttg
    13561 tttcacctcg tttaagatac gagcgttgcg ttgttcaggg attacacgtt ctcttgggta
    13621 gccaccgccc atttcttcag caaataagcg ttcaaacatc aatttgaggg tcagttctgc
    13681 cccccagcca aatcctttgg cataagggat agacaaggcg ttaccaccat tgatttgaga
    13741 ataaaggtaa gcgtcagttg ggtccactgc tagaccacag acaacaccag ggaagctgtt
    13801 taaagcaagc atagccccta cacccgtacc acagccggta acaacaaagt caactgcctt
    13861 tgtatttaaa aggatggcag ccattagtcc gttctgcaca taagttaatt gactttctcc
    13921 ttcttctcca cgcataccat agttaaataa ttggtagcct tttttatctg tcgcttcttt
    13981 tagactatcg taaataatgt gattcttgct agcttgacta ttttcattga ttaatgcgat
    14041 tttcataaga tcttctttct atttttactc aggttgtttt ccgatgtagg ctaagatacc
    14101 gccatctaca tataggatgt ggccattgac aaaattgctg gcatcactag cgagaaagac
    14161 agcagggccc atcaaatctt caggatttcc ccaacgtgca gcaggtgttt ttgcaatgat
    14221 gaactggtca aatgggtggc gagaaccatc ttcttgcaac tcacgaagag gtgctgtttg
    14281 aggagtggca atataacccg gtccaattcc gttacattgg atattggctc caccgtattc
    14341 agacgcaatg ttgcgggtca acattttcaa gcccccttta gcagcagcat aagcgctaac
    14401 tgtttcacgt cccagttcgc tcatcatcga acaaatattg ataatctttc catgcccttt
    14461 ctttatcata gaaggaataa ctgcctttga aacgataaat ggtgcgttta agtcaatatc
    14521 gatgacctta cggaaatcag cggcgctcat ttcgcacatt ggaactcggc ggataatacc
    14581 agcgttatta acgaggatgt caatgacacc aacctcttgt tcgatttgct tgaccatggc
    14641 ttggataccg tcctcgtctg tcacgtcaca gacatatcca tgggcttgga tgccaacttc
    14701 acgataagct tcaatccctt tattgaccag atcttgattg atatcgttaa agacaatagt
    14761 agcgccggct tcagcgtagg atttggcaat agcaaatcca attccataag aagcgccggt
    14821 gatgagagca atttttcctt ttaaagaaaa ctgctcaatt gagaatgatg tatttgtcat
    14881 ttgatacttc ctttcaaatt tgttaagact tctctataac caaattatat tctagtaaac
    14941 cggttttgtc aataaataaa tatttttatt tataaaaaga tttatt
//
LOCUS       DAVLRT010000044        14140 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLRT010000044 DAVLRT010000000
VERSION     DAVLRT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14140
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.044"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            258..557
                     /locus_tag="VXM85_001360"
     CDS             258..557
                     /locus_tag="VXM85_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1345929.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            776..1853
                     /locus_tag="VXM85_001361"
                     /pseudo
     CDS             776..1853
                     /locus_tag="VXM85_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2169..3143)
                     /gene="pta"
                     /locus_tag="VXM85_001362"
     CDS             complement(2169..3143)
                     /gene="pta"
                     /locus_tag="VXM85_001362"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1345930.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(3187..4083)
                     /locus_tag="VXM85_001363"
     CDS             complement(3187..4083)
                     /locus_tag="VXM85_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1345931.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(4080..4898)
                     /locus_tag="VXM85_001364"
     CDS             complement(4080..4898)
                     /locus_tag="VXM85_001364"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1345932.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(4882..5553)
                     /locus_tag="VXM85_001365"
     CDS             complement(4882..5553)
                     /locus_tag="VXM85_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1345933.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            5681..6250
                     /locus_tag="VXM85_001366"
     CDS             5681..6250
                     /locus_tag="VXM85_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1345934.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            6363..7322
                     /locus_tag="VXM85_001367"
     CDS             6363..7322
                     /locus_tag="VXM85_001367"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1345935.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            7332..8447
                     /locus_tag="VXM85_001368"
     CDS             7332..8447
                     /locus_tag="VXM85_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1345936.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            8452..8799
                     /locus_tag="VXM85_001369"
     CDS             8452..8799
                     /locus_tag="VXM85_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1345937.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            8807..9022
                     /locus_tag="VXM85_001370"
     CDS             8807..9022
                     /locus_tag="VXM85_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1345938.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            9105..9746
                     /locus_tag="VXM85_001371"
     CDS             9105..9746
                     /locus_tag="VXM85_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1345939.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            9764..10453
                     /locus_tag="VXM85_001372"
     CDS             9764..10453
                     /locus_tag="VXM85_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1345940.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(10450..11130)
                     /gene="radC"
                     /locus_tag="VXM85_001373"
     CDS             complement(10450..11130)
                     /gene="radC"
                     /locus_tag="VXM85_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1345941.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(11173..13464)
                     /gene="pcrA"
                     /locus_tag="VXM85_001374"
     CDS             complement(11173..13464)
                     /gene="pcrA"
                     /locus_tag="VXM85_001374"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1345942.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            complement(13656..14140)
                     /locus_tag="VXM85_001375"
                     /pseudo
     CDS             complement(13656..14140)
                     /locus_tag="VXM85_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4339 a         3028 c         2655 g         4118 t
ORIGIN      
        1 cgaaccttta gtaaaatcat ttccatgaaa ctagaataga gcccctctta gcaaaaatca
       61 ttattttaat ttatttctaa tcactccttg acataaataa ctctcaccaa taaaagacta
      121 tgtcttaaaa aaatggtata ataaaatcaa tacctgggct tgatggctat gctactaata
      181 acaattagga gagaaaatca ggcacttgtt aacaacaagg attatcccct tgagatgaaa
      241 ggaactttag aaatcttatg atgaacatgc aaaacatgat gcgtcaagca caaaaacttc
      301 aaaaacaaat ggaacaaagc caagctgaac ttgctgctat gcaatttgtt ggcaaatctg
      361 ctcaagatct tgtccaagcg accttaactg gcgataagaa agttgtcagc attgatttca
      421 atccagctgt cgttgaccca gaggaccttg agactctttc tgatatgacc gttcaagcca
      481 tcaactctgc tcttgaacaa atcgatgaaa ctactaagaa aaaactgggt gctttcgctg
      541 ggaaattgcc attctaaaaa caaggagcta gaacaatact tgtcgataac aaaggctaag
      601 aaaggtgcaa aaattataaa aaacgcatag tatcaagcaa cctaaaactt tgggctctat
      661 aatatttgta gtgggtaaat ccactataga tattatggag cctattttat tgtagaaaaa
      721 aatcccataa gatctataat gaaaagcgac caaacaactc attagaaaga ttcatatggg
      781 acaattacat tttatcacaa aattactaga cattaaagac cctaatatcc agattttaga
      841 catcatcaat aaggatacac acaaggaaat catcgccaaa ctggactacg acgccccatc
      901 ttgccctgag tgcggaagtc aaatgaagaa atatgacttt caaaaaccgt ctaagattcc
      961 ttaccttgag acaactggta tgcctactag aatcctcctt agaaagcgtc gattcaagtg
     1021 ctatcactgt tcaaaaatga tggtcgctga aacttctatc gtcaagaaga atcaccaaat
     1081 ccctcgtatc atcaaccaaa agattgctca aaagttaatt gaaaagattt ctatgactga
     1141 tattgcccat cagctttcca tctcaacttc aactgttatt cgtaagctca atgattttca
     1201 ctttgagcat gatttttcgc gtcttcctga gattatgtct tgggacgttg aaacagtccg
     1261 gggagtgact gtttcaatcg ggagatggag atgagcttta ttgctcagga ctttgataag
     1321 ctcaatatca taactgttct tgagagcaga acacaagcca tcatccgaaa tcccatgaat
     1381 acaaggctat caagcgctac tggaaactca ttcaacagga tagccgtaaa ctgagtgata
     1441 agcgatttta tcgtcctact tttcgtatgc atttaaccaa taaagagatt ttagacaagc
     1501 ttttgagcta ttcacaagac ttgaaacatc actatcagct ctatcaactc ttgctgtttc
     1561 actttcagaa taaggaaccg gagaaatttt tcggactcat tgaggacaat ctaaagcagg
     1621 ttcatcctct ttttcagact gtctttaaaa cctttctaaa ggacaaagag aaaatcgtca
     1681 acgcccttca actaccctat tcaaacgcca aattggaagc gaccaataat ctcatcaaac
     1741 ttatcaagcg caatgccttt ggttttcgga actttgaaaa cttcaaaaaa cggattttta
     1801 ccgctctgaa tatcaaaaaa gaaaggacaa aatttgtcct ttctcgagct tagcttttct
     1861 tcaacccact acagttgaca aagagccaga aaacgcataa tagcaggtgt caagacactt
     1921 aatattatgc gttttctcat gaaaaaattc tcttttcata ctcatttaaa atcaaaaagg
     1981 gaaattatcg ttttacggac atacatatta cagatttcta taggtcgtgt atagacagga
     2041 tatcatcgct tggaagtttt cctttttgac accaatcagt aatttagttc tcaggaatta
     2101 atggcagtgc ttttcgtaca tagtttcata actacagtat agcatagtat atcgctttat
     2161 agttttcact attgatgaac tgcttgagct gctgtgatga gggtcaactt gtaaacatca
     2221 tctgcattac atccacgaga aagatcatta actggcttgt ttaaaccttg caaaacaggt
     2281 cctacagccg caaagccacc caggcgttca gccatcttgt aaccgatatt tccggcctcg
     2341 atacctggga agatgaagac atttgcttga ccagctaccg tacttccagg agctttcaga
     2401 gctgcagttt caggaacaaa ggctgcatca aattgcaact caccatcgat ttcaaggtca
     2461 ggacgcaagt cgtgagcaat tttagttgct tcaacgacct tatcaacgct ttcaccaaac
     2521 cctgaacctt tagtagaata gctcaacatg gcaattttag gttcgatgcc aaacatctta
     2581 gctgtgattg ctgagttgat ggcaatttca gccaaggctt ctgcatctgg attgatgtta
     2641 atggcacagt ctccaaatag gtaacgttcc gtaccacgaa ccatgaggaa ggctcctgaa
     2701 gtacgagtta catttggacg agttttgatg atttgtagag ctggacgaac tgttgaagct
     2761 gttgagtgaa tcgctcctga caccattcca tcaaccaagc ccaagtaaac caacatcaca
     2821 ccaaagtagt tgacatcttc aaccaaaacc ttgcgtacat cttcttcagt cattttgccc
     2881 ttgcgacgct ccaccaaggc agaaaccatt tcttcaaatt gaggataatg ttgagggtcg
     2941 atgacctcat aaccatccat gattccttca atttcaagat aaattttaat tttttcagga
     3001 tttccaagca aaacaggaat cacttctgtt tcttttacta agcgttttgt tgcttgaaga
     3061 atacgaggct cttccccttc agggagaacg atacgagcat ttttaccaac aaggttggct
     3121 ttgagacttt caaaaacttc catgagtttt ctcctttaag ataataatta tactctgaga
     3181 cagtttttat agagtattag ttgataactg ggtaataagg ttactgaaat catccggcaa
     3241 gggactttct aactgcaagt cttgctctaa aaatggatga taaaaggata ggtaatggca
     3301 atgcagagcc tgacgttgaa taccatcttc cagactacca ccatacaaat catctcccag
     3361 caaaggaaaa ccgatatgag aaaaatggac tcggatttga tgggttcgac cagtgtgcag
     3421 gtgaatatag accaagtgaa tatttccata agaagctaca atcttgtatg aagtatgggc
     3481 atactttccg cctttagcca ctcgtctggt aataatggaa tcttcatcac gcgcaatcgg
     3541 agcaataatt tccccttctg gctccaaatg tccatctccc ttaaccaaag caaagtagcg
     3601 tttctcgata gatttcttct gcaactgctt gtctaatcgt gcatgggcat aaccgtgctt
     3661 ggcaaagagc atcaagccag aagtgtccct atctagtctg gtaacaatgt gaacctgctg
     3721 attttcataa ttttgcttga catagtaacc cttgataaaa ttggcaatgg tgttagagtg
     3781 attgacacta ggaatagaag ccactccata gggtttattc aagactagaa agtggtcatc
     3841 ctcatagaga atatctaatg gatgctcaat agcctccaag gtttcaaagc cttcctcagc
     3901 gggaatgtca atggtaacgt agtctccaac gtccaataga tacgttgcat tttgcggttg
     3961 attattgacc agaatagctc cacctcgaaa cttaatctta gccagcaatc ccttagaaac
     4021 ctcgtgcttt tttaaaaagg tcttaacctt gacatgttca tctgcgataa attcaaacct
     4081 cattcatcca cctcaccgat aaaggcatcc ttaacacggt tccagaaact ggtatggcta
     4141 ggagtcgcga caaagtgaat cttatgatgg tcgatttgat actcaatacg ctcaatatta
     4201 cggaaagaat aaacgctatt gtcaaccgaa atagtatgat aatcgtttct tgttggaata
     4261 agttcaatct tatccttctt aggcacaata atggaagagc ccagtgttcg ataaacacga
     4321 ttattaaggc tggcaatttc cgttaattgc aaagcttcaa tggtagggtg taaaacagca
     4381 ccgccaagag acttgttata ggcagtacta ccagtcggtg tcgaaactgt tagcccgtct
     4441 ccacgaaaac gttcaaaggg aacaccattt attacaatat ctgccaccat ggttcgatca
     4501 gacctgcgga tgctggcttc gttgagtgct ctgaaaatct taacttcacc attttcaaga
     4561 aagaccttca cattcagaac agggtaagaa acccttgccc cagtatctag ctgcaaatta
     4621 gtcactagct tgtccaactc aaaatcacga taatctgtat agaagcccaa atgtccagta
     4681 tgaagaccga taaagcggac cttgtcaagc tgattttcgt acttatgaaa ggccgacaag
     4741 agcataccat ccccgccaat ggaaatgaca atatccggat tggtatcatt gagtataaac
     4801 tgatttctct tcaaacgatc tcgcaattca tacaaaaccc tttgactctg cggttttcta
     4861 ttggctatca gatcaattcg tttacctgta ttcttcatct gtatcgtcac tgtttcctac
     4921 accgtcattt aattttctac tcaaaggatc aaaaagtgcc tgggcttctt ggatatcatc
     4981 acgaatttca cccatttctt catccaactg atgggcgatt ctagctgtaa tttccagtcg
     5041 cttcttaatc tcatctggga aatccccttg gtacttgtag ttgagagaat gttctatcgt
     5101 tgcccagaaa ttcatggcca aagtacgaat ttgaatttct gccaaaatag tcttagctcc
     5161 attgatggta tcaaccgtat attctactac cacatgatag gaacgatagc ctgatgcttt
     5221 tctatgagta atgtaatctc gctcctgtat gattcgcata tcctgacgct tgtgcaaaat
     5281 atccactact tccttgacgt catctacaaa ctgaaccatc acacgtaagc cagcaatatc
     5341 ctgcaaatcg tgttccaagg tcgcataagt aatgccacga cgagccattt tttctttgat
     5401 gctctcaatt ggcttgactc gaccggtcac aaactcaatt ggagaatgct tattttgctt
     5461 acgatattgc ttacgaatac cacgaagttt aatctttaac tcaccaacag cttgaatgta
     5521 aggatctaga aattcttccc attctaaggt catatattct cccttgttct catattatcc
     5581 gtcaaaaata tctttgattt tttctccaga ttcatataca ataaatacaa tgcttattat
     5641 accataaatc cgctttcaac gataaaaaaa ggtaagaaaa atgaaacatt tagaaattga
     5701 attgaaaaca ctattgaaaa aagatgaata caatcgtcta aaagaccagt tcacaggtgt
     5761 cactcctgtt cttcaaacaa attactacat cgacacgcct gattttgaac tgcgagaaaa
     5821 gaaagtcgct atgcgcattc gaacctttga agactgggct gagttgacac tcaaagtccc
     5881 gcagagtgtt ggaaacatgg aatacaacca aaaattgcaa ctaaaagatg ctgaggacta
     5941 tctgagtaag gaagaacttc ctcaaggact agtactggat gaattggcga aacatggtat
     6001 ccaaagtaag aactggcagg ttcttggttg tctaacaacg cttcgctatg aaatgaaaac
     6061 agctattggt ctcatggcac tggatcagag ccgatacttt gatataacag attacgaatt
     6121 ggagcttgaa gtggaaaatc atgagcaagg caaacaggat ttccgacaat ttttagagaa
     6181 aaatcagatt tcctaccaaa aagctccttc aaaattggtt cgatttgtca aaagtatgaa
     6241 aaatagctga aataatctcc attttttggt aaaatagaaa agataaaaat acacaaatcc
     6301 cagttcatat atgtaaggta aatataacta ggatttataa agtttacaga ggacggtcta
     6361 taatgtcaga tagaaaaaac atgaaacttt tcgcactcaa ctctaaccaa gagattgcac
     6421 aaaaaattgc ccaagctgtt ggtgtcccac ttggaaaact atcatcacgt caattttcag
     6481 acggagaaat ccaagtaaat atcgaagaaa gtgtccgtgg ttatgatgtt tacatcatcc
     6541 aatcaacaag tttccctgtc aacaaccacc taatggaatt gttaatcatg gtcgatgctt
     6601 gtgtgcgtgc aagtgcccac agtatcaacg ttgtccttcc atattttggc tatgcacgtc
     6661 aagaccgcat tgcttgtcct cgtgagccac ttacagcaaa actagttgcc aatatgctgg
     6721 ttaaggctgg agttgatcgt atcctgactc ttgatttgca tgccgttcag gttcaaggtt
     6781 tctttgatat tccagtggat aatcttttca ctgttcccct attcgcaaaa cattactgcg
     6841 ataaaggatt gcttggttca gatgttgttg tcgttagccc taaaaattca ggtgtcaaac
     6901 gtgcgcgtag cctggctgaa tatcttgatg ctcctatcgc cattatcgac taccctcaag
     6961 acgatgcaac tcgtaacgaa ggttatatta ttggtgatgt tgaaggtaag aaagctatct
     7021 tgattgatga tattttaaat acaggacgta ccttctctga agcttctaaa atcgttgaac
     7081 gtgaaggagc tacagaaatt tatgctgttt ctagccacgg tctcttcgtc gagggagctg
     7141 ctgagcttct tgacaatact aatattaaag aaattcttgt gactgattca gtagcaacaa
     7201 aagaaaaaac tcctaaaaat gtatgctaca tcactgctag tgagttaatt ggtgatgcta
     7261 tcgtccgtat tcacgaaaga aaaccagtca gcccactctt tgcctacaat aaaaagaaat
     7321 aaggtgattc tttgatttat ttggacaatg ctgcaacgac tcctatgtca gcagttgcta
     7381 tttcagctat gaccaaggtt atgcaagaaa cccacggaaa tccttctagt attcatggtc
     7441 atggtcgtca agctggcaaa ctcttgcgag aagcccgtca ggaactagcc cagttactaa
     7501 ggacaaaacc tcaacatatc tttttcactt ctggtgggac tgaaggcaat aatactacca
     7561 tcattggcta ctgtcttcgt caccaagaac aaggaaaaca tatcatcaca actgccatcg
     7621 agcaccatgc tgtccttgaa acaattgatt acttggttca acactttggg tttgaagcaa
     7681 ccattatcca gccagaaaat caagaaatca cagcccagca aattcaaaag gctttacgtg
     7741 acgatacgat tttggtttct accatgtttg ccaataatga gacaggaaac ctactgccca
     7801 tcgctgaaat tggccaaata ctcaagcaac accctgctgc ctatcatgtt gatgcagttc
     7861 aggctattgg taaaatccca attcattcag aagaattggg cattgatttt ctcactgctt
     7921 ctgcccacaa attccatggt cctaagggaa tcggttttct ctacgcatct agcatggact
     7981 ttgattccta tctacatggc ggagaccagg aacagaaaaa acgtgcagga actgaaaatc
     8041 tgcctgctat cgtaggcatg gttgcagccc taaaagaaga cctagaaaaa caagaagaac
     8101 attttcaaca tgtacaaaat ctagaaaccg cctttctggc agagctggag ggcattcagt
     8161 attacctgaa tagaggaaaa catcatctcc cttatgttct caatattgga tttcctggtc
     8221 agaaaaatga cctcttactc cttcggctag atttagctgg aatttcaatc tctactggct
     8281 cagcctgtac tgcaggcgtt gtccaatcca gccatgttct tgaagccatg tatggcgcaa
     8341 attcagaacg cttgaaggaa tcccttcgca tcagtttgtc gccacaaaat accgttgaag
     8401 acctacaaac cctcgcaaaa accttaaaag aaattatcgg aggttagcca tatggcattt
     8461 gaaaaaatca ttcagttaaa aaattgtcgt tacgattaca ctcttagccc ttctgttaaa
     8521 aaattcaccc tcaaagataa cacctttttt gaaactaagg ttggtaacta tgaactgact
     8581 cgccttttgg aaaaagtgcc aaacagcggt gaaggcttcc aactcaaaat catcattaac
     8641 aaggaactta caggggctaa aatcaatatc actgacaagt ttagccttcg tctagttgat
     8701 attttcaaat cagaagacca ccatattcat caggaaaaat tctacttcct catggatagc
     8761 ttggtagaac gtggtgtctt tacaaaatcg gaaagataga gccaaaatgt ttgaactgac
     8821 ctataaggac tgctatcatg tagagcgtac tctcaagtat gaggatcatg aggctctcat
     8881 gttgactttg tcaggatgtg taaccttacc agatacactt tatgtgactt ctctgacttt
     8941 taggggccaa aaagtttatc agggtctggt cggagacctc taccgttttc tatctcatgc
     9001 agatttttta catcaaaact aagatttttc ttagtttttt cttatttttg ttgatttttt
     9061 cacaatgttt ctataaaata gaagaataga aaggttgtga ttttgtgaaa gataaacagt
     9121 ttgctattcc aaaagctaca gcaaaaagac tctctctcta ctatcgaatt tttaagagat
     9181 ttcatgcaga aaagattgaa cgtgccaact ctaagcaaat tgcagaggct atcggtattg
     9241 attcagcgac cgtacgtcgt gatttttcct attttggtga acttggtcgt cgtggttttg
     9301 gctatgatgt caaaaaacta atgacatttt ttgccgattt gctcaatgac aactctatta
     9361 ccaatgtcat gctggttggt attggaaata tgggccatgc ccttctccac taccgcttcc
     9421 acgaacgtaa caagatgaag attatcatgg cctttgacct agacgaccat cctgaagtcg
     9481 gtacccaaac tcctgacggg attcccattt acgggatttc tcagatcaag gataaaatca
     9541 aggatgctga tgtgaagact gctatcctaa ctgttcccag cgtcaagtca caagaggttg
     9601 ctaatctctt ggttgatgct ggtgtgaaag gaattctcag tttttcacca gtccatctgc
     9661 atttaccaaa agacgtggtc gttcagtatg tcgatttgac aagtgaactc caaaccctcc
     9721 tctacttcat gcgaaaagag gattagaaag cgaaaatcat tttatgaaaa aaccagttat
     9781 tgggattaca ggaaacgaaa aagctcatcc agatgatgac atcatgatga gctacgcagc
     9841 aaaaggcttt gttgaaggcg ttaaagatgc tggagggatt cccatcatcc taccgattgg
     9901 tgatcaagaa atggcctgcc actatatcag tttgattgac aagctcatct tgacaggtgg
     9961 gcaaaatgtc gatccaaaat tttatggcga acccaaaacc attgatagcg atgactataa
    10021 ccttcaaaga gatatcttcg aactggccct catcaaggaa gctattaaac agaaaaagcc
    10081 cattttctct gtctgccgtg gtacccaact ctttaacgtt gccatgggtg gaactttgta
    10141 ccaagatatc gaagaccatt ggcaggattc ttctgttgag tacacaactc aacgcttggt
    10201 aacagaacca gataccgttc tccaagaaat ctatggagaa atctctcata tcaactcctt
    10261 ccaccaccag agcatcaagg acttagcacc aaatttaaaa gttgtggctc atgatcctaa
    10321 agatggtatc attgaggctg tcatgagtac ggatgatgtc gcctttctcg gtgtccaatg
    10381 gcatccagaa cttctttttg aaaatcgtcc caaagataaa aaactctttg actatgtcgt
    10441 taatgaactt tagattaaat ctgtcttttc acgataacta aagtaattag aatgagagac
    10501 aatcaaatgg tccaagagaa caatccccat caattcgcag gcttctttaa caagtttagt
    10561 gacatgatca tcattttggc taggcgctac cgctcctgaa ggatgattgt ggaccaagat
    10621 aagagaagtc gccatatgct tgattgcata gtgaagaatc tctcgcggtt cagcgatact
    10681 acgagttaca gaaccgataa aaatggtctg ctgatggatg atttgatttt gagtattgag
    10741 atagagtgcc accaggtgct cttgtttttt atcccctaat tcctgctgca tcttcttggc
    10801 caacttttga ctgctgagaa tactttccat ttcaagagtc tcgtgtttgt gaatacgatg
    10861 ccccagttca atcatagctt gtaattctat ggccttaaca cgcccaatac cagacaaact
    10921 ctgcaattcc tgcagggtca tttttttcaa atccgttagg cttgaaagat tgttcaagac
    10981 tttttgggca atttcaaaaa cgctagcttg acgtgttcct gtcctgagta aaatagctag
    11041 caactcttgg ttactaagcg cttcaactcc ttccttggcc agcctttctc ttggtaatag
    11101 tgaatcttct tggaatgaaa tactgtacat aaaaatcctc ctcactttat tattcgtgag
    11161 aaggatggaa aattagattt ttttctcaat tggagccaca ctggctaaaa gttttttcaa
    11221 acctacttct gggaaattga ttttcaattc ctgcctagca ccgctacctg aaacttccag
    11281 aacagttccc tctccccatt tcttgtggag agcaatgtca ccaatggacc aatttgcctc
    11341 gctagatgct gattttgcgc cagctgtaaa ttgaccaaat ggaagaccgc ttgactggat
    11401 cgattttggg gcagcaccgc gtttacggtc ttgaagagcc tgagccaaac tcataccttg
    11461 accaaaggaa atactaccac tgctatatga tgccttaaag cttgtatttg caggacgagc
    11521 cagaccttga tactcaagca agtctgaact gatttcgtta ataaaacgag tcggacggtt
    11581 ataattggta cgaccaaaaa gcaagcgtga gttggcattg gtcagataga gaattttctc
    11641 tgcacgcgtg atacctacat aggctagacg gcgctcttct tctaattcat ctggatcttc
    11701 agtcgcacga ctaagtggaa agacattttc ttccatccca atcaaaaaga caactggaaa
    11761 ttcgagacct ttggcagcat gcagggtcat caaggtcact tctgatgtct cctgactacc
    11821 tgaatctgtg tcggcaatca aagccaagtc atttaagaaa cgactcagtt tgtccagacc
    11881 agtttcctct tctgtcacat cagtggtgtc atcaaagttc ttcgtaacag aaagaaactc
    11941 ttcgatattt tcaacccttg ccttgctttc tagagtcgct tgggcgttaa gaatatcgac
    12001 ataacctgtt ttttctagga cggactcaac caactctgta atgcttaagt ggtctagctg
    12061 ctcccgcaaa tcaagcatca tattggcaaa atcccagata gattgggctg ccttaccctt
    12121 gataccagac aacataatat tagcagaagc atccagcata gacatatttt gcaaatttgc
    12181 aaaatcacgg attttctcaa ctgtacctag accaattcca cgtttaggct cgttgataat
    12241 acgctcaaaa ctaatgttgt cactcaaatt agcaataagg ttgagataag caataatatc
    12301 gcgaatttcc ttacggctgt agaatttggt tccgccaacc atggtataag gaatgttaga
    12361 cttgagcagg gcttcctcaa ttgtacggga ctgggcatta gtccgataga gaactgcaaa
    12421 atccttatga aggaagtttt gactgcgact aagttcatcg atggttctgg ctacaaatac
    12481 agcctcatcc agctcatcat cagcacgata gtaaacgatt tgctccccat cagcgttttg
    12541 agtccagaga tttttaggac ggcgattttt attattttta ataacctcgt tggccgcttg
    12601 gagaatggtt ttggttgagc ggtaattttc ctccaacaaa acaaccttag ctttggggta
    12661 atccttttca aagtccaaga tattctgcat atcagcacca cgccaaccgt agatagactg
    12721 gtccgcatcc ccaaccacac agatattttt aaaacgggaa gccaagagtt tgaccaattg
    12781 gtactgagcg tggttggtat cttggtactc atcaacgtgg atgtattgga atttttgctg
    12841 gtagtaggtc aaaacatcag gattttgatc aaagagacgc aaggtcagca taatcaaatc
    12901 atcaaagtca acggattcag actgacgaag ttctttttga taggttgtat aacactgggc
    12961 cacaatttgc gtatacatat cgccagcttg agcagcataa gcaacatcat caatcaaatc
    13021 attcttagca ttggaaatgg tccccaaaat agttcgttca ttccattttt taggatccaa
    13081 gttcaactgt ttgagaatac gtttcatgag cgttcgctgt tcaccaggat ccacaattgt
    13141 aaaattacga ttgtagccaa tatggtccgc atcgcgacgc aaaatacgca cacacatgga
    13201 gtggaaggtc gcaatcagac agtcctgagt agctggattg aggctataag cacgctcttt
    13261 catctcacgc gcagccttgt tggtaaaggt aatggccaag atattccaag gattgaccag
    13321 cttttcatca atcaaataag cgatacggtg ggttaaaaca cgagtctttc cagaaccagc
    13381 tcctgccatg attagcaagg gaccttctgt cgtttgcacc gcctcagcct gacggtcatt
    13441 cattccattt aataatgcgt tcatcttctc ttccttactc ttgaagtcaa tatttctatt
    13501 atatcagaaa tcagggaaaa tagaaacttt acggggagta gcaaaatata gtggattgaa
    13561 actagaatac tacactgtgg gtttctaaaa cactcgactt tttcacccaa ttttttcata
    13621 aactagctca atacctcaat agacttcctg caaaactaga atcctagttc atgattgata
    13681 atgccagcaa tcaaattcat tcgtaatccg aagcgtttac gatgatttcg ataggttgtt
    13741 gaaaacattt taaacgtttt tactttggca aagatgttct caaccttgct tctctccttg
    13801 gatagcgcat ggttataggc tttatcttca gctgttagcg gcttgaattt gctggattta
    13861 cgtggagttt gtgcttgagg atatatcttc atgagccctt gataaccact gtcagccaag
    13921 atttttccag cttgtccgat atttctgcga ctcattttga acaacttcat atcatgacaa
    13981 tagttcacag cgatatccaa agaaacaatt ctcccttgac ttgtgacaat cgcttgagcc
    14041 ttcatagcgt ggcatttctt tttaccagaa taattcgcta attctttttt tagggcgatt
    14101 gatttttact tccgtcgcat caatcattac cgtgtcctca
//
LOCUS       DAVLRT010000045        14002 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLRT010000045 DAVLRT010000000
VERSION     DAVLRT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14002
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.045"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(116..2374)
                     /locus_tag="VXM85_001376"
     CDS             complement(116..2374)
                     /locus_tag="VXM85_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1345943.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGGVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2603..2833)
                     /locus_tag="VXM85_001377"
     CDS             complement(2603..2833)
                     /locus_tag="VXM85_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1345944.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3023..3706
                     /locus_tag="VXM85_001378"
     CDS             3023..3706
                     /locus_tag="VXM85_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1345945.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3706..4440
                     /locus_tag="VXM85_001379"
     CDS             3706..4440
                     /locus_tag="VXM85_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1345946.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4594..5451
                     /locus_tag="VXM85_001380"
     CDS             4594..5451
                     /locus_tag="VXM85_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1345947.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAMDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5593..6465
                     /locus_tag="VXM85_001381"
     CDS             5593..6465
                     /locus_tag="VXM85_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1345948.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6747..7430
                     /gene="rpiA"
                     /locus_tag="VXM85_001382"
     CDS             6747..7430
                     /gene="rpiA"
                     /locus_tag="VXM85_001382"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1345949.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7444..8655
                     /locus_tag="VXM85_001383"
     CDS             7444..8655
                     /locus_tag="VXM85_001383"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1345950.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8657..9199
                     /locus_tag="VXM85_001384"
     CDS             8657..9199
                     /locus_tag="VXM85_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV1345951.1"
                     /translation="MTRYSLLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9215..10024
                     /locus_tag="VXM85_001385"
     CDS             9215..10024
                     /locus_tag="VXM85_001385"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1345952.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGGFKGLLKAILAEL"
     gene            10500..10895
                     /locus_tag="VXM85_001386"
     CDS             10500..10895
                     /locus_tag="VXM85_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV1345953.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10905..11480
                     /locus_tag="VXM85_001387"
     CDS             10905..11480
                     /locus_tag="VXM85_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1345954.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11552..12016
                     /locus_tag="VXM85_001388"
     CDS             11552..12016
                     /locus_tag="VXM85_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1345955.1"
                     /translation="MELEGYNALSLAEVEVFRFIATNAETATQVSKPVQPISQTPVKD
                     KTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGCNQTAGFVLNLPIKENM
                     RNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTLNSKVSDDDVL"
     gene            12674..13162
                     /locus_tag="VXM85_001389"
                     /pseudo
     CDS             12674..13162
                     /locus_tag="VXM85_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
     gene            13166..13393
                     /locus_tag="VXM85_001390"
                     /pseudo
     CDS             13166..13393
                     /locus_tag="VXM85_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
     gene            complement(13416..13721)
                     /locus_tag="VXM85_001391"
                     /pseudo
     CDS             complement(13416..13721)
                     /locus_tag="VXM85_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
     gene            13769..>14002
                     /locus_tag="VXM85_001392"
     CDS             13769..>14002
                     /locus_tag="VXM85_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1345956.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCP
                     ECGSQMKKYDFQKPSKIPYLETTGMPSRILLRKR"
BASE COUNT         4169 a         2582 c         3131 g         4120 t
ORIGIN      
        1 agattcagtc tactatatat cataatggac aaagggcaag ctgtcaagaa attacagtta
       61 ttttagtaaa aaaagaagca gaaaaatttc attttctact ccttttctat aggatttatt
      121 tttctttttc agaactttta acttcagctt ttttggcaga tttaatctga atgtttccct
      181 tgctagtcat aactgctttg agatcttttt cgcttggatt ttcaaggtag tagtcagtga
      241 ttgtgtcctc aatatagtct tgaatagtcc gacgaagtgg gcgtgctccc atttttggat
      301 cataacctag gtcaaccaac ttttccttga ccttatcagt tacatccaaa cgaatgttgt
      361 tgctagagag gcgcttgtta acatctgcta gcatgagctc gacaatctga aggaggttat
      421 ccttgctgag agccttaaat tcgataatgc catcaaaacg gttcataaac tctgggctaa
      481 agaagttacc gagttcaccg aggacagaat tggtacgtcc ttctctagca gcaccaaatc
      541 caacgctagc ttcggtcttt cctgtacctg catttgaggt catgataatg atggcatcct
      601 tgaagctaac ggtgcgtcct tgcccgtctg tcaaacgacc atcgtccaag acttgaagaa
      661 acatgtgcat aacatctggg tgagcttttt ccacttcatc gagaaggatg agagaatatg
      721 gattgtggcg aactttttca gttaattgac cagcctcatc atagccaaca taacctggag
      781 gggcgccgac caacttagcc acactatgtt tttccatgta ttcactcata tcaaagcgaa
      841 tcatactatc agcagaacca aaaagttcga tagccagttg tttggaaagt tctgtcttac
      901 cgacaccagt tggcccaacg aagaggaagc ttccgattgg gcggttaggg gtaccaagtc
      961 cgacacgatt acggcgaata gccttggcaa tcttatcgac tgcatcatct tggccaataa
     1021 catgagactt gagatcttcg gctagatgga tgagttgaga ttgttctttc tctttcaaat
     1081 caccaacagg gatattggtt ttctgctcga taatgtgctc aatagttttc tcgctgatga
     1141 taggagtatc ctggtctgtg atctttttct tttgcatttc cttatacttg gcaatctggt
     1201 cgcggaagta ggccgccttc tcaaaatctt cttctcgtgt agcttgagac ttgagatttt
     1261 cagcctcaat caagcgctga tcaattactt taggatccac aaaattcaag gtcaagttca
     1321 tcttagaacc agcttcatct aggaggtcaa tggccttgtc aggcaagaag cgatcttgga
     1381 tgtagcgatt ggaaagagtt gcagctgctt caatcgcagc atcggtatat tgaacgtggt
     1441 ggtaatcttc gtatttcttt tgaatccctt tgagaatagt gattgtttcg tccaccgttg
     1501 gttcatcgac tttaacaggc tgcatacgac gctcgagggc agcatccttt tcaatgatac
     1561 ggtattcatt gagggtagta gcaccgacta gttgcagttc tccacgagca agggctggct
     1621 tgaggatatt tcctgcgtcc atattaccat cactcgcaga accagcacca acaatttcat
     1681 ggatttcatc gataaagagg atgatgtctt cacgtttgcg aatttcttcc atgagttttt
     1741 gcatgcgttc ttcaaattgt cctcgaatcc ccgttccttg aactaagcta accacatcca
     1801 gacggatgac ttgtttacct tggagtttat gtggcacacc gccatcgaca attttctgag
     1861 ctagaccttc gacaacggcc gtttttccga caccaggttc accgataagg acaggattat
     1921 tcttggttct acgattgaga atctcgatga cacggataat ctcatcgtcg cgcccaataa
     1981 cggggtcaat gtctccacga cgggcaattt cagttacatt aataccaaat tcttccagca
     2041 ggcctttttc ttggctaggt ggcggagttt gagcagatcc acgattttgg gaaccataac
     2101 cgccgtttcc accgtaacct ccacctgatt gggttggggg aataggagga gtattgctag
     2161 aaggtctgaa attgtttaga tcattgaaga aatcaccaaa gggatcgaag tcacgattgt
     2221 tcagatccgt catacctttg aagaggctat tgttaggatc tgttttgata atcttatagc
     2281 agttttgaca gaggtcaatt tgtttttgtt ttccattgag attggtgtaa agatgaattg
     2341 ttgagtcgtt aattttacag ttttgacaaa gcatacatct acctcatttc tttctttagc
     2401 ctgacttgtt taaaggtcaa aaatagtcaa attctatact ctcattataa caggattggg
     2461 gtgtaaagca agtaaaaaga ttgaagcttt gagaagttgt tacaatgaaa atttttgtga
     2521 atttggacta taaaaaaatc ctatttgtga gacaaaaagg attttactta gacatgcagg
     2581 gacaatccca gcttgtttta gattagtaga gaagttcgta aatttccatt gcgattaagt
     2641 caatgctatc aaacgtatac gtttcgcgat cttctacgtc acgaagggta aagattgggc
     2701 cgttttcttc gtcgtggctg tacgcaactt ctgcaacaac aactccttcg cgttcaaaat
     2761 tacgttttaa atttccgcca tcgtttgcca ttgcttcaag gcggttgatg attctaacca
     2821 aatgtgattc cattttgatt ctccttcatt tcttatcgtt actattttac cataaaggag
     2881 gccaacttga ctagaaaaaa ctaagatttc tcactaattc tgctagttta aagattttta
     2941 aataaattgg ataaaagatt taaattttaa ttcaaaacat gttataatca tacagtattc
     3001 tattttagaa agcagtgtga ctatgaattt ttctttttta cctaagtatt taccttattt
     3061 taactatggg gctgttgtga cgattcttat ttctatctgt gttatctttt tgggaactat
     3121 tttgggtgtt gtcttggctt ttgggcaacg ttcaaagttt aaaccgcttg tttggttggc
     3181 caacttgtac gtttggattt tccgtgggac accgatgatg gttcaaatta tgattgcctt
     3241 tgctcttatg catatcaatg ctccgactat tcagattgga attttaggtg ttgatttttc
     3301 gcgtctgatt ccagggattt tgattatctc tatgaatagt ggtgcttatg tttcggagac
     3361 tgttcgtgcc ggaatcaatg cggttccaaa aggtcagcta gaggcggctt attcgctagg
     3421 gattcgtcct aaaaatgcga tgcgttatgt gattttgcca caagcagtca aaaatatctt
     3481 gccagcattg gggaacgaat ttatcaccat tatcaaggac agctccctct tatcagctat
     3541 tggggtcatg gagttgtgga atggggctac aacagtttct acaacaacct atctaccttt
     3601 aacaccactt ttatttgcag cattttacta cttgattatg acctctattc tgacagtagc
     3661 cttgaaagct tttgaaaaac atatgggaca aggagataag aaataatgac agaaaccttg
     3721 ataaaaattg aaaatttaca taaatctttt ggaaagaatg aagtattgaa gggcatcaac
     3781 ctcgagatta aaagaggaga agttgtcgtt atcatcggtc cttcagggag tgggaaatct
     3841 accttgcttc gctctatgaa tttgttggag gaagcaacca aggggaagct tatctttgag
     3901 ggagtcgata ttacggacaa gaagaatgac ctgtttgcca tgcgtgagaa gatgggcatg
     3961 gttttccaac aatttaacct ctttcctaat atgactgtga tggaaaatat taccttgtca
     4021 cctatcaaga ctaaaggtga aagcagggaa gttgctgaga agagagccca agagcttttg
     4081 gaaaaagttg gtttgccaga taaggcagac gcttatccac agagtctgtc gggtgggcaa
     4141 caacaacgga ttgccatcgc gcgtgggttg gctatggaac cagatgtttt gctctttgac
     4201 gaaccaactt cagccttgga tcctgaaatg gtaggtgagg tattggctgt tatgcaagac
     4261 cttgccaagt cagggatgac tatggttatc gtaacacatg agatgggatt tgcccgtgag
     4321 gtggcagatc gtgttatctt tatggcagac ggtgtggttg ttgaagatgg gacacctgag
     4381 cagatttttg aacaaaccca agaacaacgg actaaagatt tcttgagtaa ggttttataa
     4441 tctattttaa aatttcaaga caagattagt gaaaagttca accagagctt tttcttatag
     4501 tttgaaacta taggataacc taggaaagaa gtgttagagg gcactagcag tttttggtat
     4561 aatggaagat atttgaaaga aaagagaagt gatatgacac agattattga tgggaaagct
     4621 ttagcggcca aattgcaggg gcagttggct gaaaagactg caaaattaaa ggaagaaaca
     4681 ggtctagtgc ctggtttggt agtgattttg gttggggata atccagccag ccaagtctac
     4741 gttcgcaaca aggagaggtc agctcttgcg gctggttccc gtagcgaagt agtgcgagtt
     4801 ccagagacca ttactcaaga ggaattgtta gacctgattg ctaaatataa tcaggatcca
     4861 gcttggcatg ggattttggt ccagttgcca ttaccaaaac atatcgatga agaggcggtt
     4921 ttattagcca tggatccaga aaaggatgtg gatggtttcc atcccctaaa catgggacgt
     4981 ctttggtctg gacatccagt catgattccc tcgacacctg caggaattat ggaaatgttt
     5041 catgaatatg ggattgactt ggaaggtaaa aatgcggtcg tcatcggtcg ttcaaatatc
     5101 gttggaaaac ctatggctca gcttcttttg gctaaaaatg cgacagtaac cttggcccac
     5161 tcacgaactc ataatcttgc caaggtggct gctaaagcag atattcttgt agtcgcaatc
     5221 ggccgcgcca agtttgtgac tgctgacttt gtcaaaccag gtgcggttgt cattgacgtt
     5281 gggatgaatc gagatgaaaa tgggaagctc tgtggagatg ttgattatga ggcagttgca
     5341 ccacttgcta gccatatcac gccagtccct ggaggtgtcg gtcctatgac cattaccatg
     5401 ctgatggagc aaacttatca agcagcactt cggacattgg atagaaaata agagaaacat
     5461 tcgattaata gagtgtattt tctatagcta tatctaaaat ggcagaaatg aatattaaat
     5521 tttagatata agattacaaa aggaggtctg cgtctccttt ttattgtata ataaaagtga
     5581 gaggaaaaaa ggatgaaagt gattaatcaa accttattag aaaaagttat tattgaacgt
     5641 tctcgttcaa gtcataaagg agattatggt cgtctgctgc tgcttggtgg tacttatcct
     5701 tatggaggtg ccatcatcat ggctgcttta gcagctgtaa aaagcggtgc aggattggtg
     5761 accgttggaa cggacaggga aaatatcccg gctctgcaca gccatttacc tgagcctatg
     5821 gccttttctc ttcaagacca gcaattgtta aaagagcaat tggagaaggc agaagttgtc
     5881 ttgctggggc ctggtttacg agacgatgct tctggagaaa atctagtaaa acaggtcttt
     5941 gttaatttaa gccaaaatca gattttgatt gtagacggag gtgccttgac cattcttgct
     6001 aggacaagtt tgtcatttcc ctcaagccag cttatcctaa ctcctcacca aaaagaatgg
     6061 gaaaaattat ctggaattac gattgaaaag caaaaggaag atgcaacggc tagtgttctg
     6121 acttccttcc ctcaaggaac aattttggtg gagaaaggcc cagctactcg tatttgggaa
     6181 gttggccaat ctgattatta ccagttacag gttggtggtc cttatcaggc gactggggga
     6241 atgggagata ctctggctgg gatgatcgca ggatttgtag gccaattccg acaagccagt
     6301 ctctacgagc gtgtggcagt agcgacccat cttcattcag ccatagccca agaactatct
     6361 caagaaaatt atgtggtctt accgacggaa atcagcaggt atcttcccaa aataatgaaa
     6421 attatttgtc aacaagagag ggtaagtaaa gataaacttg tttaaaatag acagataaat
     6481 ctacttcctt ctcttaattt atgattcttg aaatgcgcct gctttaaggc taaactagac
     6541 gtatcaaagt taagattttg tttttgtaac tggaaagaga tgaacaaatc aaatacataa
     6601 aagataaact catttttatt cgtttggtaa aaataaaatg catattttta aagaaagatt
     6661 gatgaaaaat gtagatttga tgtttttact tgaataaaaa gcggtttcat agtaaaataa
     6721 gaactgagaa tgatggaggt aaatcggtgg aaaatctgaa gaaaatggca ggtatcaagg
     6781 ctgctgagtt cgtgagcgat ggaatggtcg ttggacttgg aacaggctcg actgcctatt
     6841 attttgtcga agaaatcggt cgtcgtatca aggaagaagg attgcagatt acagctgtaa
     6901 caacttctag tgtgaccact aaacaggcag aaggccttaa tatcccgctc aagtctattg
     6961 accaagtaga ctttgtcgat gtgacggttg atggcgcgga tgaagtggat agtcagttta
     7021 atggaatcaa aggcggtggt ggtgcccttc tcatggaaaa ggtggtcgca acaccatcaa
     7081 aagaatacat ttgggtggtg gatgaaagca agctggtcga aaaactaggt gcttttaaat
     7141 tgccagtaga agtggttcag tatggtgcag agcaggtctt tcgtcatttt gaacgagctg
     7201 gctacaaacc aagtttccgt gaaaaagacg gccaacgttt tgtgacggat atgcagaatt
     7261 ttatcattga cctcgccttg gatgtcattg aaaatccaat tgcttttgga caagaattgg
     7321 accatgtcgt tggtgttgtg gagcatggtt tattcaacca aatggtggat aaggtaatcg
     7381 ttgctggacg agatggagtt cagatttcaa cttcaaaaaa aggaaaatag aagggggcat
     7441 aagatgtcta aatttaatcg tattcatttg gtggtactgg attctgtagg aatcggtgca
     7501 gcaccagatg ctaataactt tgtcaatgca ggggttccag atggagcttc tgacacactg
     7561 ggacacattt caaaaacagt tggtttgaat gtcccaaaca tggctaaaat aggtcttgga
     7621 aatattcctc gtgaaactcc tcttaagact gtagcagctg aaagcaatcc aactggatat
     7681 gcaacaaaat tagaggaagt atctcttggt aaggatacta tgactggaca ctgggaaatc
     7741 atgggactca acattactga gcctttcgat actttctgga acggattccc agaagaaatc
     7801 ctgacaaaaa tcgaagaatt ctcaggacgc aaggttattc gtgaagccaa caaaccttat
     7861 tcaggaacgg ctgttatcga tgattttgga ccacgtcaga tggaaactgg agagttgatt
     7921 atctatactt cagctgaccc tgttttgcag attgctgccc acgaagacat tattcctttg
     7981 gatgaattgt accgtatctg tgaatacgct cgttcgatta cccttgagcg tcctgccctt
     8041 cttggtcgca tcattgctcg cccttatgta ggtgaaccag gtaacttcac tcgtacggca
     8101 aaccgtcgtg acttggctgt atctccattt tccccaactg ttttggataa attgaatgag
     8161 gctggtatcg atacttatgc tgtgggtaaa atcaacgata tctttaacgg tgctggtatc
     8221 aaccatgaca tgggtcacaa caagtcaaat agtcatggaa ttgatacact attgaagact
     8281 atgggacttg ctgagtttga aaaaggattc tcattcacaa acctagttga ctttgatgcc
     8341 ctttacggcc atcgtcgtaa tgctcacggt taccgtgatt gcttgcatga gtttgatgaa
     8401 cgcttacctg aaattatcgc agctatgaga gagaatgacc ttctcttgat tactgcggac
     8461 catggaaatg acccaacgta tgcaggaacg gatcacactc gggaatatat tccattgttg
     8521 gcctatagcc ctgcctttaa aggaaatggt ctcattccag taggacattt tgcagatatt
     8581 tcagcgactg ttgccgataa ctttggtgtg gaaactgcta tgattgggga aagtttctta
     8641 gataaattgg tataagatga cgcgctattc tttgctggtg agaggtatca atgttggtgg
     8701 taagaataag gtcgtcatgg cggagcttcg tcaagaattg acaaacttgg gactggaaaa
     8761 ggttgagagc tacatcaaca gtggcaatat tttctttact tcgatagatt ccaaagccca
     8821 attggttgaa aagctagaga ctttctttgc agtccattat ccatttattc agagcttttc
     8881 tttactgagc ctagaggact ttgaggcgga acttgaaaat ctaccagctt ggtggagcag
     8941 agacttggca cgaaaagatt ttctctttta cactgagggt ttggatgtgg accaagtcat
     9001 cgcgacagtt gaaagtttag agctgaaaga tgaagtgctt tattttggaa aacttgggat
     9061 tttctggggg aaattttctg aagaatccta ttctaagact gcctatcata agtacttgct
     9121 gaaggtgcct ttctaccgcc acattactat tcgtaatgct aaaacctttg acaaaattgg
     9181 tcaaatgcta aaaaaataat aaaggagaca cacaatgaca tttttaaaca aaatccatga
     9241 aactgctact ttcctgaaag aaaagggaat tgcagcccct gagttcggtc taatccttgg
     9301 atcaggactt ggagaattgg cagaagaaat cgaaaatcca gttgtagtag actatgctga
     9361 gattccaaac tggggccgtt caacagtagt cggtcatgct ggtaaattgg tatatggtga
     9421 actggcaggt cgcaaggtct tggctcttca agggcgtttc catttctatg aagggaatcc
     9481 tctggaagtg gtgactttcc cagttcgtgt gatgaaagtt cttggatgtg aaggtgttat
     9541 tgtaaccaat gcagctggcg gtatcggatt tggtcctggt accttgatgg ctatctcaga
     9601 ccatatcaac atgacggggc aaaatccatt gatgggtgaa aacttggatg actttggccc
     9661 acgtttccca gatatgtcta gggcttacac accagaatac cgtgccactg cccatgaagt
     9721 ggctaaaaaa cttaatatca agcttgatga aggtgtctat atcggagtta ctggtccgac
     9781 ttatgaaaca ccagcagaaa ttcgttccta taagacactg ggagcagatg cagttggtat
     9841 gtctacggtt cctgaagtta tcgtggcagc ccactctggc ttgaaagttc tgggaatttc
     9901 atgtatcact aactttgcgg ccggtttcca agaagaactc aatcacgaag aagttgtaga
     9961 agtgactgaa cgtgttaaag gtggtttcaa aggcttgctt aaagcgattc ttgctgaatt
    10021 gtaagaaaaa agatttaaaa gggggagtgc ctctgtcttt tcaggattga ctgcctatcc
    10081 gtattaaaga agaaacagag gaatactatg agcttcttcc tgctcttata actgaaagaa
    10141 gcggaagaat aggtatgtct gatctgatag ccagcattgt gaaagacaag attctaggat
    10201 actagcatta gcttcctagc caagcagact agtatgataa ggagagatga gaatgaattg
    10261 actttctgaa tttctcagtc ttatcatata tagcacaatg agatttcgct tgagtctgct
    10321 tgtaaataaa cgaaaagaaa gataagaaat aatgaaaatt ggtcaacgaa ttatgcgctt
    10381 tggcataaaa aattaagtat cggagttgta tctgttgtag tcggctttga tttctagctc
    10441 cagctggaat ttcagccaat gaagtaaagc aagatgtaac atctgaagtg gtaataggtg
    10501 tgctagattc taaggaggaa ttgaaagagt cagaaaatga tgctccaaaa ctagaaactc
    10561 ctcttagaga ggagccaaga ctagctcctc aaacgcttcc ggaagcaagt gaagttcttg
    10621 aaaacaaaag ggaagagtca aaagtagaga taacagagcc agctcaagcg gatgatatcc
    10681 gcaaggttgt tggggaatta gccaaggata taagtattac taagttgtat atgacaggtc
    10741 attctcttgg aggctaccta gctcagattg cagcggttga agattaccaa aaatatcctg
    10801 atttttataa ccatgtattg aggaaagtga caactttcag tgctcctaaa gtgattactt
    10861 ccagaactgt ttgggatgct aagaatggtt tctgagatgt tggtttggaa agtcgtaaat
    10921 tagctgttag tggaaaaatt aagcattatg tggttgataa tgacaatgtt gtgactccct
    10981 tgattcataa taatcgtgat attgttacat ttacaggtaa ttcacgcttt aaacaccgtt
    11041 ctcgtggcta ttttgaaagt ccaatgaatg atattcctaa ctttaatatt ggtaaacaag
    11101 ctaccttgga taaacatggt tatcgtgatc cgaaattgga taaagtgcga ttctttaaga
    11161 aacaggctct acctcaatct tctagtcaac caagcgctga accaatggaa aatattgcct
    11221 taggaaaaca ggttactcaa agttcgacag ctttcggagg agatgctaga agagctgtgg
    11281 atggcaaagt cgatggtaac tatggtcaca attctgtcac tcatacaaac ttccaatcta
    11341 agccttggtg gcaagtagat ttggctaaag aagaaaccat tcgccaaatc aatatttaca
    11401 accgaacaga cactgcccag gatagattgg caaactttga tgtcattctt ttagacagtt
    11461 ctggtaaaga aattgagtga aaacgtataa tatctcctaa agatgtgtca gcacaaatta
    11521 cgattaacca taaaaaacgc gctatgttcg gattgagcta gaaggctata atgccctcag
    11581 tcttgcagaa gttgaagttt tccgctttat agctacgaat gctgaaacgg cgacacaagt
    11641 ttctaagcca gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat tgacaattca
    11701 acacagtgga gcttacattg cccgctactc cataacttgg gaagaagttc cagtagataa
    11761 agatggaaac caagttgttc gtagtcattc ttgggaagga aacggttgca accagactgc
    11821 aggttttgtc ctcaacctcc caatcaaaga aaatatgaga aatctgcgag ttaagattga
    11881 gaaaaagacg ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttt
    11941 agctcaaccc caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga
    12001 cgatgatgtc ttgtaatctg atggtacaat gacagttagt ttgtctagtt tataagaaag
    12061 tactacctga gcttgaatag aactcaggta gctctctgtg aaagaacaaa attaatactc
    12121 aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt tgctcaaagc actgctttga
    12181 ggttgtagat aagactgacg aagtcagtca catatataat ccaaggcgac gttgacgtgg
    12241 tttgaagaga ttttcgaaga gtataaacag aaaggtagag cgcgtgttct aatttgaaca
    12301 cgagtagaaa acttttctaa aaacaaaaac gaaaggatgg gtaaactgta ttcgctgaac
    12361 tgaatacggg cgactctcct ctaaatcaaa attaagaaag aaaggaattg accccaccct
    12421 aaaagcagtg ggaaaaagat agttggtcta gcgagcatcg ctcactgcgc ccaactccta
    12481 ttttcccttc gctttttgac gggtttggta tctttctcaa tataaaatat aaaataaaga
    12541 aaggtagagc gtgtgttttg atttgaacac gagcggaaaa ctcggaaaat agataatctg
    12601 actgaaaaat caggatttct cgtcggcttc ctaattttca gtcgttttct tctcgctctt
    12661 tgtatcataa attatgtcta tccatattgc tgctcagcag ggtgaaattg ctgataaaat
    12721 tcttcttcct ggggatcctc ttcgtgctaa gtttattgcg gagaatttcc ttggtgatgc
    12781 tgtttgtttt aacgaagtgc gtaacatgtt tggttacact ggtacttaca agggtcaccg
    12841 tgtatctgtc atgggaactg ggatgggaat gccatctatt tcgatttatg cgcgtgagtt
    12901 aatcgtagac tacggtgtga agaaattgat tcgtgtggga actgcaggtt ctttgaatga
    12961 agaggttcat gttcgtgaat tagttttggc gcaggcggct gcaaccaact caaacatcgt
    13021 tcgtaatgac tggccacagt acgattttcc acaaattgct agctttgatt tgcttgataa
    13081 agcctaccat atcgccaaag aacttggtat gactactcac gttgggaacg ttttgtcatc
    13141 tgatgtcttt tactcaaatt actagcgtta tcttaaatac cgtggaaaag tcagagaaag
    13201 tatggcggca gattgccctg aacgctttga ctgggagttt atcagatgga ttctttggga
    13261 tggacgtagc aaaactcaaa aagaaaatta ccaaaaactt tgccaagaat attcacataa
    13321 agtcactatt cttcgaaatc agagagagct agatcaattt ctggataaga aaaggaagtc
    13381 ctacaattca taaagggctt cctttttggc tataattatt ctgcaatcaa ggtttccaaa
    13441 ccaaccttca tcatatcagt gaaggtattt tgacgttctt ctgcagttgt gtcttcgtct
    13501 ggattgacca agctatcaga gatggtcatg atagctagcg catcaacatg gtattgggca
    13561 gcaagatagt aaagagctgc tgcttccatt tccacagcct tgactcccca tttaccaagc
    13621 tcgatattct tttcaaagta atttggctct ataatatttg tagtgggtaa atcccttata
    13681 gatattatgg agcctatttt tgtgtagaaa aaaagtccca tatgacctat aatgaaaagc
    13741 gacaaaacaa ctcattagaa agaatcatat ggaacaatta cattttatca caaaattact
    13801 agacattaaa gaccctaata tccagatttt agacatcatc aataaagata cacacaagga
    13861 aatcatcgcc aaactggact acgacgcccc atcttgccct gagtgcggaa gtcaaatgaa
    13921 gaaatatgac tttcaaaaac cttctaaaat tccttatctt gaaacaactg gtatgccttc
    13981 tagaattctc cttagaaaac gc
//
LOCUS       DAVLRT010000046        13623 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLRT010000046 DAVLRT010000000
VERSION     DAVLRT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.046"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..1655
                     /locus_tag="VXM85_001393"
     CDS             <1..1655
                     /locus_tag="VXM85_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEV1345957.1"
                     /translation="NRQLFTNIDNMNDWFIDATKDHVYIAERASEVEEIKNAKYRAYA
                     NLNVAQHRKMILPLLNITKSHMFIASNYNAIAIGSAEKLGKKSLEDIKASVDYAADGY
                     RNYYDFWYRLVPDNVKQYLLRDTVIPIWEGYNAPGGWVEKYGRYNTDNVYTPLREFFG
                     PIEKYYGYNGTGAYAALYDNPMDPRSDVSYVALNMIGDYGISVYTHETTHINDRKIYL
                     GGFGRREGTDAEAFAQGMLQLPVPGSGFNEYGSLGLNTVFKKPNDGNQWYDTDPKSLT
                     TRDDIDKYMRGYNDALMLVDHLEADAVINQNNKELNGAWFKKIDKKFRDASQLNQWDE
                     IRNLTTEEKDNLNITSIDSLIDQQLMTNRQIGNRVYKPEVVSQYDQSPYVGVEMTAGI
                     YGGNTSKGAPGAVSFKHNAFRLWGYYGYLNGFLGYTSNKYKQQSTKDGESVLSDEYII
                     KKISNNTFNTIEEFKKAYFKEVKDKATKGLTTFEVNGSSVSSYDDLLTLFKEAVKKDA
                     ETLKQEANGHKTVSMTNTVKLKEAVYKKLLQQTDSFKTSIFR"
     gene            1716..3437
                     /gene="pabB"
                     /locus_tag="VXM85_001394"
     CDS             1716..3437
                     /gene="pabB"
                     /locus_tag="VXM85_001394"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1345958.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            3438..4055
                     /locus_tag="VXM85_001395"
     CDS             3438..4055
                     /locus_tag="VXM85_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1345959.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            4105..5103
                     /locus_tag="VXM85_001396"
     CDS             4105..5103
                     /locus_tag="VXM85_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1345960.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLTSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTV
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            5230..6189
                     /locus_tag="VXM85_001397"
     CDS             5230..6189
                     /locus_tag="VXM85_001397"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1345961.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTVGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            6286..7125
                     /locus_tag="VXM85_001398"
     CDS             6286..7125
                     /locus_tag="VXM85_001398"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1345962.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRIDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(7173..7343)
                     /locus_tag="VXM85_001399"
     CDS             complement(7173..7343)
                     /locus_tag="VXM85_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1345963.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            7464..8348
                     /gene="miaA"
                     /locus_tag="VXM85_001400"
     CDS             7464..8348
                     /gene="miaA"
                     /locus_tag="VXM85_001400"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1345964.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHFVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            8341..9579
                     /gene="hflX"
                     /locus_tag="VXM85_001401"
     CDS             8341..9579
                     /gene="hflX"
                     /locus_tag="VXM85_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1345965.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKFQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDS
                     EVITGYISEKNKWRLEEFYD"
     gene            9572..10195
                     /locus_tag="VXM85_001402"
     CDS             9572..10195
                     /locus_tag="VXM85_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1345966.1"
                     /translation="MTDIKALALKYGGYTSLDKVYLVQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     DSDGLGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            10210..11139
                     /locus_tag="VXM85_001403"
     CDS             10210..11139
                     /locus_tag="VXM85_001403"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1345967.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTVRPRKISKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTNASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            11160..11915
                     /locus_tag="VXM85_001404"
     CDS             11160..11915
                     /locus_tag="VXM85_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1345968.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(11969..12877)
                     /locus_tag="VXM85_001405"
     CDS             complement(11969..12877)
                     /locus_tag="VXM85_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1345969.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLSHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            12941..13210
                     /locus_tag="VXM85_001406"
     CDS             12941..13210
                     /locus_tag="VXM85_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1345970.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            13210..13590
                     /locus_tag="VXM85_001407"
     CDS             13210..13590
                     /locus_tag="VXM85_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1345971.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILRARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT         4124 a         2563 c         3070 g         3866 t
ORIGIN      
        1 ataatagaca attattcaca aatatagata atatgaatga ttggtttatt gatgctacaa
       61 aagaccatgt ctacatcgca gaacgcgcat ctgaggtcga agaaattaaa aatgctaaat
      121 atagagcata tgctaactta aacgtagctc agcacagaaa aatgatttta ccattattga
      181 atataactaa atcacacatg tttattgcct ctaactacaa cgcaattgca attggtagtg
      241 ctgagaaatt aggaaaaaaa tcattagaag acattaaagc atctgttgat tatgctgctg
      301 atgggtacag aaactactat gatttttggt atcgtctagt tccagataat gttaaacaat
      361 atctactaag agatactgtg attcctatct gggaaggtta taatgcgcca ggtggctggg
      421 ttgaaaaata cggtcgatat aatactgaca atgtatatac tcctcttaga gaattctttg
      481 gacctataga aaaatattat ggttataatg gaacaggagc atatgctgca ctttatgata
      541 atccaatgga tcctcgttct gatgtaagtt atgttgctct aaatatgatt ggggattatg
      601 gtatttctgt gtatactcat gaaacaacac atataaacga tcgtaaaatc tatttgggtg
      661 gttttggacg tcgtgaaggt actgatgctg aagcatttgc tcaaggtatg ttacaactac
      721 cagtcccagg aagtggattt aacgaatatg gttcattagg tctgaatacc gtattcaaaa
      781 aacctaatga tggtaatcaa tggtacgata cagatcctaa atctcttaca acacgtgatg
      841 atatcgataa atatatgcgt ggttacaatg atgctttaat gcttgtcgat catttagagg
      901 cagatgctgt tattaatcaa aataataaag agttaaatgg agcatggttc aagaaaatag
      961 ataagaaatt ccgagatgcc agccaattaa atcagtggga cgaaattcga aatctaacaa
     1021 ctgaagaaaa agataactta aatattacat caattgatag tttaatagat caacagttaa
     1081 tgactaatag acaaattgga aaccgtgttt ataaacctga agtcgttagc caatacgatc
     1141 aatcacctta tgtgggggtt gaaatgacag ccggtatcta cggaggtaat actagtaaag
     1201 gtgctcctgg agctgtttca ttcaaacata acgcctttag attatggggt tactacggat
     1261 accttaacgg attcctaggc tatacttcga ataaatataa acaacaatct acaaaagatg
     1321 gcgagtcagt tctaagtgat gaatatatta tcaagaaaat atctaataat acattcaaca
     1381 ctattgaaga atttaaaaaa gcttatttca aagaagttaa agataaagca acgaaaggat
     1441 taacaacatt cgaagtaaat ggttcttccg tttcatcata cgatgattta cttacactat
     1501 ttaaagaagc tgttaaaaaa gatgctgaaa ctcttaaaca agaagcaaac ggtcacaaaa
     1561 cagtatctat gactaataca gtaaaactaa aagaagctgt ttataagaaa cttcttcaac
     1621 aaacagatag ctttaaaact tctatcttta gataatcgaa atctctcatc tgctttgcgg
     1681 atgagggttt tcttatttta tgctatactt aaggtatgca tagaaaaaca gtgattgatt
     1741 ttaggacttt aggggagaga tataccttta cccagcctat taaagagttg aaaacgagaa
     1801 atgtagaaga agtggcagat ttgctggcac aagtggaaag ctaccaagag caaggttatt
     1861 atgtggtggg gtatgtcagc tacgaggctg cacctgcttt tgaggaaaaa ttagcagttc
     1921 acaaggatcc cctactggga gagtacctgc tttattttac tgttcatgat agcgtagaaa
     1981 cttcccctat tcctctgact tatgaggata ttgatttgcc ctcaaattgg caggaagtaa
     2041 cgtctgcagc agactatgaa aaggccattg cccagataca ccatcacttg aggcagggag
     2101 acacctatca ggtcaactac accgtccaac tcaagcaaaa gttaagtgcc aatccttttg
     2161 ccatctacaa tcgtatggtg gtagagcagg aggcgggcta caatgcctat gtggaacatg
     2221 acgagatggc agtgatttcc atgagcccag agctcttttt tgagcaaaat gatcgcgagt
     2281 tgacaacacg accaatgaag gggacgactc agcgtggggt aactgaccaa gaagatcttg
     2341 aacaggccag ttggttggaa caggatccca aaaatcgctc tgaaaatatg atgattgtgg
     2401 acctcttgcg caatgatatg aaccgtattt ctgaagttgg gagcgagcac gtggagcgtc
     2461 tgtgccaggt agagcagtat tcaactgttt ggcagatgac ttcgaccatc aagagtcagt
     2521 tgcgagagga tgtggacctt gttgaaatct tccgctcact ctttccttgt ggttccataa
     2581 cgggtgcacc gaaaattgcg acaatggaga ttatcaagaa cttggagcct caaccgcgtg
     2641 gagtctactg tggaacgatt ggtctcttgc ttccaaatgg acgacggatt tttaatgtgg
     2701 ccattcgtac cattcaactt cacaaaggtc aagccattta tggagttggc ggagggatta
     2761 cttgggatag cacatgggaa tctgaatacc gagaggttca tcaaaaggca gcagttcttt
     2821 atcgtaaaca agctcgcttc caactgatta cgacagggga aatcagccag aagaacctgc
     2881 tgtttgaaga tcaacatctg gaaagactga gaaaagctag tcgttatttt gcctttcctt
     2941 ttgatgcaga agacttggga cacaagattg aggaagagtg tcaggattgt gaagctaatc
     3001 aagattaccg cttgcgaatc agccttagca aatctggaga gatagaagtc aatcgtcaag
     3061 tattaacccc tctcagtaca agcttttgtc aggcccaagt ctgccttcag gaagctgctt
     3121 tgaatcaatc ctttacctac tttaaaacca ctcaccgacc gcatttgagc ctaggagaac
     3181 aagagaagat ttaccacaat aagtcaggag aactgcttga aacctctata ggaaatttgg
     3241 ttctgaaaat cgctggaaaa ctctacacac cgcctatccg acttggaatc ttgccaggaa
     3301 tttaccgtca gcatttgcta gaaacaggac aggtagaaga gaaagtcttg accttggcag
     3361 atttaaccca agcagaagct atttacggct gtaatgcagt gagaggcttg tatgaactaa
     3421 gccttgagga gaactagatg aaattgatat ttttgcatgg gttagggcag tcagcagagt
     3481 cttggaaaga agttcggaat ctactgacag attatccttc tgaggctatc gagttatttc
     3541 cttctggagt tagtaactat caacaagcta aagagcgagt ttatcagcac ctagctcaag
     3601 agacagaacc ttttgttttg attggattgt ccttaggtgc tgccctgaca ctagaacttt
     3661 ccagttacga tttaccaaat cttcgagctt tgattctgtc aggatgcccg cttaaattag
     3721 cagggaatat cctattttat cttcagttgc tgatttttaa actacttccc aaaagagtat
     3781 ttgaaaaaca gggagcagac aagactctta tggtcggagt ttctgaggaa ttgaagacac
     3841 ttgatttaac ggatatagca gggacttgct cctatccaac cttactaatt tgtggcagca
     3901 aagataaacc gaatcttagt tctatgaaag ctctccatag actgttaaca gattcgcagt
     3961 ttcagatcat tcctgatggc cctcatgtct tgaataaggc aaaaccaaag gagtttgtag
     4021 aaaagaccag aagtttcctt gaattgctga aataagtttg ataaaataat attgaaatcg
     4081 atatcataat tatgggagaa agaaatgaat aaacgtctat ttttaaaaat gagtctggtg
     4141 acgttgccaa ttttagcctt gttttcacaa ccagttttgg cggaagaaaa catccatttt
     4201 tcgagctgta aggaagcttg ggcgaatggc tattcggata ttcacgaggg agaacctggt
     4261 tattctgcca agttagaccg tgatcatgat ggtgtggctt gcgaattgaa aaatgctcct
     4321 aagggtgctt ttaaagcaaa acagtcaacg gctattcaaa tcaacacaag ttcagcaaca
     4381 acaagtggtt gggttaagca ggacggcgct tggtactact ttgatggaaa tggaaatcta
     4441 gtgaaaaatg catggcaggg aagctattac ctgaaagctg atggtaaaat ggcacagagt
     4501 gaatggattt atgactcttc ttatcaagct tggtattatt tgacatcaga tggttcttat
     4561 gcaaaaaatg catggcaagg agcttattac cttaaatcaa acggtaaaat ggcacaaggt
     4621 gagtgggttt atgattcttc ttaccaagca tggtattact tgaaatcaga tggttcatat
     4681 gctcgcaatg catggcaagg aaactactat ttgaaatcag atggtaaaat ggctaaaggt
     4741 gaatgggttt atgatgccac ctatcaagct tggtattatt tgacatcaga tggttcttat
     4801 gcttacagta catggcaagg aaattactat ctaaaatcgg atggtaaaat ggctgtcaat
     4861 gaatgggttg atggtggacg ttattatgtt ggcgctgacg gagtttggaa ggaaggtcaa
     4921 gcaagtacag tttcttctag taatgatagc aatagtgaat attctgctgc tttaggaaag
     4981 gcaaaaagtt ataattcgtt attccacatg tcaaaaaaac gtatgtatag acaattaact
     5041 tctgattttg ataaattttc aaatgatgca gctcaatatg ccattgatca tttagatgat
     5101 taacagatac tggtaggcga aaaaattcct tataattcat ttacgcaaac ctttgcataa
     5161 gtgaatggat tttgatatac tagtactgta agcattttca attactacac aatgaaaaag
     5221 gagaatatga tgagtcaaaa gattattggg attgaccttg gtggaacttc tatcaaattt
     5281 gcaatcttaa caacagtagg agaaatccaa ggaaaatggt caatcaagac caacattttg
     5341 gatgagggaa gtcatatcgt tgatgatatg attgagtcta ttcaacatcg tttggacttg
     5401 cttggattgg cagcagcgga cttccaaggc attggaatgg gatcaccagg tgtggttgac
     5461 cgtgacaaag ggactgttat cggtgcctac aacttgaact ggaaaaccct tcaaccaatc
     5521 aaacaaaaga ttgaaaaagc tttgggcatt ccatttttca tcgataatga tgccaacgta
     5581 gcagctcttg gtgagcgctg gatgggtgct ggagataacc aaccagacgt tgtctttatg
     5641 acactcggta ctggtgttgg tggtggtatt gtcgcagaag gcaaattgct tcatggtgtt
     5701 gctggtgcag caggtgaact tggtcatatc actgttgact ttgaccaacc aatctcatgt
     5761 acttgtggta aaaaaggctg ccttgagaca gttgcttcag caacagggat tgtcaacttg
     5821 actcgtcgct atgccgatga atacgaaggc gatgcagcct tgaaacgctt gattgataac
     5881 ggagaagaag taactgctaa gactgtcttt gatctcgcaa aagaaggaga cgaccttgct
     5941 ttgattgttt accgtaactt ctcacgttac ttgggaatcg cttgtgctaa cattggttca
     6001 atcctaaacc catcaacaat cgtcatcggt ggtggtgtgt cagctgcggg agaattcctt
     6061 ctacaaggtg ttcaaaaagt ttacgatgaa aatagtttcc cacaagtacg cacatctact
     6121 aaattagctc ttgcaactct aggaaatgac gctggagtta tcggagcagc atcacttgta
     6181 ttgcaataag ataatgagag aggaaaatgc taacctagag ttagtaatgt tcctctttta
     6241 ttttttatgc tatactagtt aggacaatga atgaggtgaa acgaaatgac aaaagcagat
     6301 acgattttta aagagaatat tgaacgcatt ctaaaggaag gtgttttctc agagcaggct
     6361 cgtcctaagt acaaggatgg gactgttgct aattccaagt atgtaactgg tgccttttcc
     6421 gagtacgact tatctaaagg agaatttcct atcacaacct tgcgtcccat tgcaatcaaa
     6481 tccgccatca aagaagttct ctggatttac caagaccagt caaatagctt agaagtgctt
     6541 aatgacaagt acaatgttca ctactggaat gactgggaag ttggagacac gggaaccatt
     6601 ggtgagcgct atggtgccgt tgttaagaaa cacgacatta tcaataagct tctcaaacag
     6661 ttggaaacca atccttggaa ccgccgcaat attatttcgc tctgggatta ccaagctttc
     6721 gaagaaacag atgggctgct cccgtgcgcc tttcagacca tgtttgatgt ccgccgtata
     6781 gatggagaaa tctatctgga tgcgaccttg acccagcgct cgaatgatat gctggtggcc
     6841 caccacatca acgctatgca gtatgtggct ttgcagatga tgattgccaa acattttggc
     6901 tggaaggttg ggaagttctt ctacttcatc aacaacctcc atatctatga taatcaattt
     6961 gaacaagctc aggaattgct ccgtcgggag ccgtcaaact gccaaccacg cttggtttta
     7021 aatgttcctg atgggactaa tttctttgat atcaaagcag aagattttga gttggtggat
     7081 tatgaccctg ttaagccaca gttgaagttt gacctagcta tttaaaagaa tagaaaaaag
     7141 aagttgagaa taatcccaac ttcttttgtt tcttaacgtg atactcggcg acgagctgct
     7201 tttttacggt tttcttcgat gaaagctgct ttttgctctt ctggttcgat tactttcttt
     7261 ttaaatgcgt atactgcacc tgcaacggca gcgacagttc ctgcgacacc tgttacaaga
     7321 cctttagcga atcctttagc catgagtctt cctcctttat attctcaatc agccagcctc
     7381 ctcaagaggt cacatttttc tgactgacct ttttgtgtta taataatagt aacgaaaaaa
     7441 tgggaatttt tcaaggaaaa aagatgaaaa caaaaataat tgtgattgtt ggaccgactg
     7501 ctgttggaaa gacggctcta gctattgaag ttgcaaagcg ttttaatggc gaagtggtta
     7561 gtggagatag ccaacaagtc tatcgaggac ttgatattgg gacagctaag gctagtccag
     7621 aagagcaggc agctgttctt catcatttaa tcgatgttag agagataacc gagtcttact
     7681 cggcttttga ttttgtttca gaagctaaga tgactattga ggatattcac agccgtggca
     7741 agctagccat tatcgccggt gggactggac tttatatcca gagcttgcta gaaggttacc
     7801 acctaggtgg ggagactcct catgaggaga ttttagctta tcgagctagt ttggagccgt
     7861 attcagatga ggaattagcc cattttgtgg agcaagcagg ccttgagatt ccccagttta
     7921 atcgtcgtcg tgctatgcgt gccttagaaa ttgcccattt tggtcaggat ttggaaaatc
     7981 aagagacctt gtatgaaccg ctgattatct gcttggatga tgaacgtagt caactttatg
     8041 agcgtatcaa ccaccgagtg gatttgatgt ttgaggctgg gcttttggat gaggccaagt
     8101 ggctgtttga ccattcccct aatgtacagg ctgctaaagg aattggctat aaggaactct
     8161 ttccttattt ccgtggagag cagacctttg aggaagctag tgagagtctt aaacaggcga
     8221 cccgtcgttt tgccaagcgt cagctgacct ggttccgtaa tcgcatgcag gtcacctttt
     8281 atcagattgg agaatctggt gtacaagacc gcattttaag ccagatagag gagtttttag
     8341 atgattgaaa cggagaaaaa agaggagcga gtcctgctga ttggtgtgga attgcagggt
     8401 atggacagtt ttgacctctc catggaagaa ttggctagtt tagcgaaaac ggcaggggca
     8461 gtcgttgtag atagctacag acaaaaacgt gaaaaatatg attccaagac cttcgtcggc
     8521 tctggtaagt tggaagagat tgcgcttatg gtggatgcag aagaaatcac tactgtcatc
     8581 gtcaacaacc gtctgacccc aaggcagaat gtcaatctag aggaagttct cggtgttaag
     8641 gtcattgacc gtatgcagtt gattttggat atctttgcca tgcgggctcg aagccatgaa
     8701 gggaagttcc aagtccacct agcccaactc aaataccttt tgcctcgctt ggttggtcag
     8761 gggattatgc tcagccgtca ggcaggggga attggttccc gtggtcctgg tgaaagccag
     8821 ctggagctga accgtcgtag cgttcgcaat caaatcacgg atatcgagcg ccagctcaag
     8881 gtggttgaga aaaatcgtgc gactgtcaga gaaaaacgtt tggagtctag cacttttaag
     8941 attggtttga ttggttatac caatgctggg aaatcaacta tcatgaacat cttgaccagt
     9001 aagacccagt atgaagcaga tgagctcttt gcgactctgg atgcgacaac caagagtatt
     9061 catctgggag gcaatctcca agtaactttg acagataccg ttggctttat ccaagatttg
     9121 ccgacagagt tggtgtccag tttcaagtca accttggaag aaagcaagca tgtggacctt
     9181 ctggttcatg ttatcgatgc tagcaatcct taccacgagg agcatgaaaa aacggttctc
     9241 tccatcatga aagacctgga catggaagat attcctcact tgacgcttta taataaagcg
     9301 gatttggtgg aggatttcac gcctacccaa acgccatata ccctcatttc tgccaagtct
     9361 gaggacagtc gtgaaaactt gcaagcatta ttgctagata agattaagga aatttttgaa
     9421 gcatttaccc tgcgagtgcc tttttcaaag tcctacaaga ttcatgattt agagagtgtt
     9481 gcaattctgg aagaacgtga ttatcaggaa gacagcgaag tgattacagg ctacatttcc
     9541 gaaaaaaata aatggaggtt agaagaattt tatgactgat attaaagctt tggctctaaa
     9601 gtatggaggt tatacaagtc tggacaaggt ctatctggtt cagcttctag ctggtaaaac
     9661 agagcaggag cagttaacac ttattacacc tccgccaagt gttgttaatg cctactttgc
     9721 agaactctat cagaaaaaga gtcctcaggc tgcgacggat tattttgcag aactcagtca
     9781 ggaactgaat ctttacaata ctgagccaag tttcacccta gaaaataagc cttttattcg
     9841 gcttaatctg tctggtaaat cctttggttt ttgttatgat agtgacggcc ttggtcggat
     9901 tttctctgaa aatgaggaga aaatctcgga tgacttgctc tttgaaattg cgcaaatttt
     9961 cccccatcaa ttagtctttg aagagtctgg taagatttac atgaagcctg tcgaggacga
    10021 ggaagttgtc agcctagaaa gtctcacagc tttgactgat ttggaaagat tggctgatgg
    10081 tcgaaaacgt ctcaaaggct acagccaaga ggatttattg caagaagctg ctgctttttc
    10141 tggcaaacgc tatttccgat cggaaaaccg cacagccatg ttatatattg attaattaga
    10201 aagtatcgaa tggatattca atttttagga acgggggctg gtcagccctc taaagctcgc
    10261 aacgtttcaa gtctcgccct gaaacttttg gatgagatta acgaagtttg gctctttgac
    10321 tgtggagaag gtacgcaaaa tcgcattctg gaaaccacag ttcgaccacg taagatcagc
    10381 aaaatcttta ttacccatct gcatggagac cacatttttg gtttgccagg tttcctttct
    10441 agccgtgcct ttcaggccaa tgaagagcag acagatttgg aaatctacgg acctcaagga
    10501 atcaagtcat ttgtcttaac cagccttcgt gtgtcaggtt ctcgtctgcc ctaccgcatt
    10561 catttccatg agtttgacca agattctcta ggaaaaatcc ttgagaccga taaattcact
    10621 gtgtatgcag aggagctgga ccatactatt ttctgtgttg gctatcgtgt catgcaaaag
    10681 gatctagaag ggacgctgga tgctgaaaaa ctcaaggctg ctggtgttcc gttcggcccg
    10741 ctttttggta aaatcaaaaa cggccaggat cttgttttgg aagacggaac tgaaatcaag
    10801 gcggctgact atatctcagc gccacgtcca ggtaagatta tcactatttt aggagacact
    10861 agaaaaacca atgctagtgt gcgtctggct gtcaatgcag atgtcctagt tcatgagtcc
    10921 acttatggca agggtgatga aaaaattgct cgtaaccatg gtcactcaac taatatgcaa
    10981 gctgcacaag tagcggtaga agcaggtgcc aaacgcctcc tactcaacca tatcagtgcc
    11041 cgtttcctct caaaagatat tagcaaactc aagaaagacg ctgccacaat ttttgaaaat
    11101 gtccatgtgg tcaaagactt ggaagaagtg gaaatctagc agtcacagaa aggataagta
    11161 tgcctactat tctcattacc ggagctagcg gtggtctagc tcaagaaatg gtcaaactcc
    11221 tgcccaatga ccaactcatc ttgcttggta gaaataagga aaaattagcc caactctacg
    11281 gaaattattc ccatgcagaa ttgattgaaa ttgatattac cgacgattca gccctagaag
    11341 ctctggtaac tgatctttat ctccgctatg gcaagattga tgtcttgatt aacaacgctg
    11401 gttacgggat ttttgaggga tttgaccaga ttgctgataa agacattcac cagatgtttg
    11461 aggtcaatac ctttgccctg atgaatctgt ctcgtcacct tgcggctcgt atgaaggaaa
    11521 gcagcaaagg gcatatcatc aacatcgtca gcatggcagg tctaatagct actggcaagt
    11581 ctagtcttta ctcagcgacc aagtttgcgg ctattggttt ttcaaatgct ctgcgactcg
    11641 aacttatgcc ctatggagtc tatgtgacaa cagtcaatcc aggtccaatc cgaacaggat
    11701 tttttgacca agctgaccca gatggaactt atcttaaatc ggttgaccgc ttcctcttag
    11761 aggcagatgc agtggctaaa aagattgtca agattatagg aaaaaataaa cgagaactca
    11821 atctcccgat tttgttgaac ctagcccata agttttatac tctctttccc aagctagctg
    11881 ataagttggc aggggaaact tttaattata agtaaaaaga accaatgtgc aggttgttgc
    11941 tagcctacat attggttctt atcatttctc aactatcaaa ctgaacttct tctagtagat
    12001 agtcgataaa gcgttcgccc atcttagata ggctggtttt ctcatgctgg atatagacca
    12061 gctcaatcgg gtcatcaata tccagtggga tagaaacgat attgtctccg tttaggttgc
    12121 tgttcaaaat ccctgtcgca atggtataac catccaaacc aatcaagaga ttaaagaggg
    12181 tggcacggtc actgaccaca atggatttct tgtggtgttc ttgagaaaga atctcttctg
    12241 aaaagtagaa ggagttgtgc gtcccttggt catagctgag gtaagggaaa ttctccaaat
    12301 cagacagttt caccttgtct ttctttgcca gagggttggt cttgctgaca aagatatgcg
    12361 gttgcgctgt gaagagatgg tgggatagca ggtgattgtc atccagcatc ttggttaaaa
    12421 catcacggtt gtaactgttt aagaagagga ccccgacctc actgcggaag ttcttgacgt
    12481 cgtcgataat ctcccaagtc cgagtttcac gaaggaagag ttcgtatttc tccatatcgc
    12541 ttttcttgag caaagagaca aaggcattga ccacaaaggc atagtgttga gacgaaacgc
    12601 taaagagttc gcggtgggcg acaggatttt tatagcgttc ctccagaagc tgagtttgct
    12661 cgacaacctg acgggcataa gagagaaact ccatcccatc acgggtcaag gtaataccct
    12721 tgggattgcg gataaagatt tcaatgccca tttcattttc caaatctcgc actgcattgg
    12781 agagactggg ctgagtgata aagagttgct tggctgcctc attcatagag ccagtttcga
    12841 cgattttgat aatataatgt aattgttgaa ttctcatgtt tttattgtac cacacttgcc
    12901 ttagaatcgc caaagaagat aggaaaataa ggagcattgt gtgagtctct tcttgaaaaa
    12961 aacacaaaat tttgctagaa tgagtcttat gaaaacattc tatgatgtgc agcaattcct
    13021 caaacgattt ggtattattg tttacatggg aaaacgctta tatgatattg aactgatgaa
    13081 gttggaacta tctcggattt acgatgcagg gttgatggat aaattagact atctagaggc
    13141 agaagcagtt cttcgcagag agcacaaggt agaattggat tatattgaga aaaatggaga
    13201 aaagaactaa tggttacttg gattttgtgg gcacttatac tagcaatgtt ggcgtggatg
    13261 ggctttaact atcttcgtat tcgccgtgcg gctaaaattg tggacaatga ggagtttgaa
    13321 gccttgattc gtacgggtca attgattgat ttgcgcgacc cagcagaatt ccacagaaaa
    13381 catatccttc gtgcacgcaa tattccttca agtcagttga aaactagtct tgcagccctt
    13441 cgtaaagata aacctgtcct tctctacgag aaccaacgtg cgcaacgagt tacaaatgca
    13501 gctctttact tgaaaaaaca aggtttttct gagatttata tcctttctta tggcttggat
    13561 tcttggaaag ggaaagtgaa gactagctaa ccttatttcc aaattagtcg aatgatagga
    13621 tga
//
LOCUS       DAVLRT010000047        13499 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLRT010000047 DAVLRT010000000
VERSION     DAVLRT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.047"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(90..851)
                     /locus_tag="VXM85_001408"
     CDS             complement(90..851)
                     /locus_tag="VXM85_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1345972.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(884..1510)
                     /locus_tag="VXM85_001409"
     CDS             complement(884..1510)
                     /locus_tag="VXM85_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1345973.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(1526..1843)
                     /locus_tag="VXM85_001410"
     CDS             complement(1526..1843)
                     /locus_tag="VXM85_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1345974.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(1840..2139)
                     /locus_tag="VXM85_001411"
     CDS             complement(1840..2139)
                     /locus_tag="VXM85_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1345975.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(2424..2615)
                     /locus_tag="VXM85_001412"
     CDS             complement(2424..2615)
                     /locus_tag="VXM85_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV1345976.1"
                     /translation="MSYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGAMIANDWVKDNGK"
     gene            complement(2776..3450)
                     /locus_tag="VXM85_001413"
     CDS             complement(2776..3450)
                     /locus_tag="VXM85_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345977.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLAIIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(3456..3902)
                     /locus_tag="VXM85_001414"
     CDS             complement(3456..3902)
                     /locus_tag="VXM85_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1345978.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(4016..4519)
                     /locus_tag="VXM85_001415"
     CDS             complement(4016..4519)
                     /locus_tag="VXM85_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1345979.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVFIPATGFVILSLLRKKINAPRPYEEWDIKPLLDRDSPGQSM
                     PSRHVFSATIISMTCLHASLTVGFILLVLSALLGLVRVLGGVHYPKDVVVGYICGLAW
                     GVLFFLF"
     gene            complement(4516..4878)
                     /locus_tag="VXM85_001416"
     CDS             complement(4516..4878)
                     /locus_tag="VXM85_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1345980.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGEAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(5004..6803)
                     /locus_tag="VXM85_001417"
                     /pseudo
     CDS             complement(5004..6803)
                     /locus_tag="VXM85_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(6842..7291)
                     /locus_tag="VXM85_001418"
                     /pseudo
     CDS             complement(6842..7291)
                     /locus_tag="VXM85_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(7415..8131)
                     /locus_tag="VXM85_001419"
     CDS             complement(7415..8131)
                     /locus_tag="VXM85_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1345981.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(9012..10427)
                     /gene="ply"
                     /locus_tag="VXM85_001420"
     CDS             complement(9012..10427)
                     /gene="ply"
                     /locus_tag="VXM85_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1345982.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(10438..10770)
                     /locus_tag="VXM85_001421"
     CDS             complement(10438..10770)
                     /locus_tag="VXM85_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345983.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(10841..11449)
                     /locus_tag="VXM85_001422"
     CDS             complement(10841..11449)
                     /locus_tag="VXM85_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345984.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(11462..11920)
                     /locus_tag="VXM85_001423"
     CDS             complement(11462..11920)
                     /locus_tag="VXM85_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1345985.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            12045..12444
                     /locus_tag="VXM85_001424"
                     /pseudo
     CDS             12045..12444
                     /locus_tag="VXM85_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13208..13468)
                     /locus_tag="VXM85_001425"
     CDS             complement(13208..13468)
                     /locus_tag="VXM85_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345986.1"
                     /translation="MRERSATGAQDLSKSIKKHLNDITRLTASLLGDEKLSAIISSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT         4067 a         3055 c         2393 g         3984 t
ORIGIN      
        1 tttattatac atattttctt cctcctactt atctattcgt aggaaatcaa aaaaagttac
       61 agaaatttgt aacttttcga ggaaatttct tattttttat gaatcatgaa acctgtcgcc
      121 tgttgattgg ccataatggt catatctgta atctgaacac ggcgaggctg actggtcaca
      181 tagactactg tgtctgcaat atcctgagct tgcaaggctt ctattccttg gtaaacggac
      241 gcagcccgct ctttatcacc atgaaaacga acagttgaga aatctgtttc gacaatccca
      301 ggctgaatgg ttgtcacctt gatatccgtt gcgatggtat caattcgcag tccatccgaa
      361 aaggtcttaa ccgcagcctt ggtagctgag taaacagcgg caccagcata ggcgtagatt
      421 cctgcggttg accccatatt gataatatga ccttgattga cttttatcat tgctggcaag
      481 aaacagcgag taaccgccat cagaccttta acattggtat ccaacatggt caacatatcc
      541 aactcctcat agtcttgata gggagccaag tcaagagcca gtccggcgtt gttgaccaag
      601 atatcaatcc tccctatcgt ttctagaata tcagagcaaa cagtcttcac catgaccata
      661 tccgtcacat ctagtggaaa agtccagacg gtttgatttg gaaagcttac tgcaaactcc
      721 gacttgagga tttctaatct gtctatccgt cgtcctgtta gaacgacatc ctcaccctgc
      781 tccagataag cacgcgcaat cgcttcaccg attcctgagg ttgctcccgt aatcactaca
      841 ttttttgcca tcttatttcc ttctatcaga tgctgacaag ttcctaggca gtccagtgtt
      901 tcgctgggtc gaacggtgtt ccgacaactt ggtcttctga taattcaagc accccacgtt
      961 tttgcggagc atttggcaaa tgcaattcac gaggactaca catcatccca aaactctttt
     1021 caccacgaag ttcgcctggg aaaatgaaat tcccttttgg catcatggca ccaggaagag
     1081 ccacaatggt tttcaaccca acacgcgcat taggtgctcc tgcaacgatt tgcactatct
     1141 tgtcacttgc gactgcaact tggcagatgt tgaggtggtc actgtctgga tgagctacca
     1201 tctcgacaat ttcaccaaca acaaacttag gttccttatc attaacaatt tcttctgtaa
     1261 aaccttccgc ctgtaattct tggttcaaac gagcgacttg ctcatctgat aaaaagactt
     1321 gaccgcgctc tgcaatttca aacaagctcg aaacctcaaa aatgttccaa gctactgttt
     1381 ccccattttc tttgaggaaa acacgggcta ccttgccttt gcgttccaca ttcagtttgg
     1441 catctccgct atttttcacg atgaccataa ggacatcacc gacatgttct ttgttatacg
     1501 taaaaatcat tgtttccttt ttctcctatt tcaatcctgc taaaaaatcg ttaatttgct
     1561 ccttactttt acggtcgcga ttgacaaaac gaccgatttc cttgtccttt tctagaacaa
     1621 caaggctagg aattccgtaa acatcccaga gtttggccaa atccatatac tgatctcggt
     1681 ccattcgaat aaaggtgaac tctggattgg tctcttcaat ctctggtaag gcaggataaa
     1741 tataacgaca atcgccacac cagtctgcca caaaaaggaa gaccttcttg cccgcttttt
     1801 ccactaaaga tgctaattct tctaaacttg ctggctgtat cataagactt cctcctcata
     1861 gactaggtct tcattttcat agacaaaggt ataatgacgg ccatcctcaa aaatgacgcc
     1921 accaagcaag ctctctagac tgctttcata gacttgaaca tagagggtcg caatttcccc
     1981 catgtctgaa aaatggtctc gcacaatctc tgtcaactct tcctgagtct tcatgagctt
     2041 acggtcatct gcaacttttt tcgtagcaag agcaaggctt ccgataccta gcagagccaa
     2101 gcctgccatc cacatttttt tagctttcat accattcatt ttaacacaaa aaaggcttca
     2161 ggacaaatga ggaagcagca gaaaagcaag taaaaagcct cttcctttaa ggaaaaggac
     2221 ttcttatact aagagcaaac tagaaagcta gccgcaggct gctcaaaaca cggttttgag
     2281 gttgtagata aggcgacgtt gacgtggttt gaagagattt tcaaagagta ttattcttat
     2341 tgccaggcac ctaagttgcc aacgtagtaa ccatcaggtg tgtaggtatt gcgaagcatc
     2401 ttacctgatg aagccagata atactacttg ccattgtctt tgacccaatc attcgcaatc
     2461 atggcaccag aagaacttac ataataccat tctcccttgt cataaaccca agtactgact
     2521 ttcatggttc ctgagcaatt aaaggcaaaa aaactgtcca atatcattcg ttttttaaaa
     2581 gcatttgaca ctacatcatc tacgctgtag gacatagatg catcaaacgt aaaagccaaa
     2641 atatcgaaaa atatacaact tttaaaggca gcatttcatt ttttaaagtc taacaaccat
     2701 agaactgaaa tcagagcaag gcatcaattt cctactctta gtacgtctat tttgatggtt
     2761 gaagtgaaga tttttttatt taacgtagac aatttccaac ttcccaaaag ccacatctcc
     2821 acggataatc aaggttttgc tggttggggc tacaggagcg tcagccttag ctgcaccaaa
     2881 ggaggtttct actttcaaat ctacacgcca gtgttgtgga acatagacgg ttgcatttcc
     2941 gaaggccact tcaatattta aagttgcaaa atcacctagc atctctgcat tatcatagta
     3001 gattttagca tccccaaaag cgacttccac ttggtcatct acgagatctt gatcttgctt
     3061 atagaaggtc ccgctaccaa aagcgacttc cttatccgtg acgactgttt ttttcccatt
     3121 gtaccaccat tttttttcat tccagaactt acttgaatgc gtcagataac caacaccaag
     3181 taccaccaag atgctagccc aaataagaga atgattggta actggtaaca agtcataagc
     3241 gtaatttgca atgatgatcg ccagtaaacc tgtaaaaact gccgaagtga gatgacgtct
     3301 aaggatggac tcaatggact tataagcaaa aaaaacaata cctagtaaag gccatatttt
     3361 accatccaaa gaaggaattc caaaattccc ttgcagcaag atccaagctg ctaaaaccaa
     3421 taaaacaata ccaaatgctt tctttttcat gttttttacc tcatgtttct tagattttct
     3481 tttaggaggg attggtaatg ccgtgagaca tgaacctcct tgtgcgtctg ataaaaggaa
     3541 atggtgcccg ttcctgaaaa ggacttgtcc actgagtaaa tctgacggat gtttgcgatc
     3601 gttgacttgg aaactcgatt aaaatagcga ggcaagatag actccaactc atagagcttg
     3661 aggcgaacct cataagcttc cttctggtta tgagcgtaga tcttgctccc ttctgtttca
     3721 aagaagagaa tttctgacag gtctagataa tattcacctg tccccttgta aaaagtcaac
     3781 ctcggagcct ttgactcttg caagagccgt tgcaagtctg caatttcatc tgtcaaagtg
     3841 ggggtcttga tgacaatttc agtctcctct aaattgccgt caatctcaat tcgtaacttc
     3901 atcacatctc ctttctgact ctactatatc aaagctaagt taggaataca agggcaaaaa
     3961 agcaagtggt caatttgaag cagtaagtgg ttgcaagacc aacttaactt acaggtcaaa
     4021 atagaaagaa aagcacaccc cacgcaagac cacaaatata accaactact acatccttgg
     4081 gataatgtac accacctaag actctcacga gaccgaggag ggctgacaag accaacaaga
     4141 taaagcccac agttagacta gcatgtaagc aagtcatgga gataatggtt gctgaaaaga
     4201 catgacgact gggcatagac tgaccaggac tatctctgtc aagcaagggt ttaatatccc
     4261 attcctcata aggcctaggg gcattgattt tcttacgaag aagggataaa atcacaaaac
     4321 ctgtagcagg gataaacaca taaaccccaa cttgctgacc aagtcctagt tgcaggtaag
     4381 tggtgactag caagatcaga tagaccaggg gcatggcgac tgtcatcaaa cgattgaaac
     4441 tgcgtaatag gaataaaaga attggacgac tagttagtct ggagctgata tttcgatacc
     4501 attcttgata atttttcatt tatgcttctc atacttttct agaacttgtt gagatgtttt
     4561 ccccttggca agccaatcca ctagcttgtc caagcgacgt atttcctgca tcagaggatc
     4621 atcaatttcc tcaatacgga caccgcaaat cttccctgta attgcaattc gttcagggac
     4681 ataatatggt gcttgtcgaa aaaaatctcc gtaagtaaca tccctatcca gacaagcaag
     4741 aacgtcactc acttcatacc ctgtcaacca actggtcact tggtgaactg actctgcctc
     4801 tccacccttt cgctcaactt ttgcaatcaa ggctaaatag acagcagcaa atttcatatt
     4861 gtataatttc tgactcatac ttctctcttt ctatgtgtac tagtataaat aagaaaaaga
     4921 aggccgtcaa gccttctttt gatttattct tctgcttcat cttctgtaaa ttgactattg
     4981 tacaagtcag cgtagaagcc accttacacc atcagttcct catagttgcc ttgctcgatg
     5041 atatttccat ctttcatgac caagatcaag tctgcatttc ggatggttga caagcggtgg
     5101 gcaatgacaa aggatgtgcg tccttccatc aaacggtcca tggctttttg gatcaattcc
     5161 tctgtccgtg tgtcaacaga agaagtcgcc tcatccaaaa tcaaaagcgg tgcatcctta
     5221 agaagggcac gagcaatagt caatagttgt ttttgtccta cagacaaggt cacggtgtca
     5281 tccaagatgg tatcatagcc atctggcaag gtcataataa agtggtgaat tcccacagcc
     5341 ttactagctt ccatcattcg ttcatcacta atccctattt gattatagat gagattgtct
     5401 cgaatagttc cttcaaagag ccaggtatcc tgcaagacca ttgaaaaggc atcatgcact
     5461 tctgaacgcg tcatagcctt ggtatccaca ccatcaatgc gaatacttcc cttatcaatc
     5521 tcatagaatt tcatcaaaag attgacaatg gttgtcttac cagccccagt cggcccaaca
     5581 atggcaaccc tttgaccagc atgagctgtc gcagagaagt catagtcttg aacattgaca
     5641 ccgtccacca gaatttctcc tgctgacacg tcgtagaaac gtggaatcag attgaccaga
     5701 gttgatttac cagaacctat tgacccaata aaggccactg tttgaccagt ttctgcttta
     5761 aagctaacat gttcaataac tgcctccgaa tttgccgcat agcggaaggt cacatcctta
     5821 aactcgacct gacctttgaa gttttcatca gtcagctgca cttgagcagg gttttggata
     5881 gaagaatgca aatctaaaac ttgattaatc cgcttagcag agaccatagt tcggggaaga
     5941 acgatgaaga gtgctcccat gagaaggaag tccatgacaa cctacatggc ataagacatg
     6001 aaaacaatca tgtcactaaa gagaggcaga cgcgctatcg gagcagcgtc gttaatcaca
     6061 taggccccaa tccagtaaat cgccacactc aaaccacttg aaatccccat catgatagga
     6121 ttcaaaatag ccataagacg gttgacaaac aaattttaac gggtcaattc atcatttact
     6181 gctgcaaatt tttcattttg ataatcctct gcattgtagg cacgaacgac acgaatacct
     6241 gttaaactct cacgagtgat actgttcagt ttatctgtca gcccctgaat caaggactgt
     6301 tttggaaagg ctagcgtcat caaaacggtc gtcatcagga cgttgacaat cactgccaca
     6361 agtacggccc agagccagta ttctgaatga cctaaaatct tcccaatagc ccagatagcc
     6421 ataattggac cacacgttac cacttgcaag tccatagtaa tcaacatttg aacttgagta
     6481 atgtcattgg tagtacgcat caagaggcta ggaattgaaa atttcttaat ctctgtctgc
     6541 gagtaatcca aaactcggtt aaaaatatca cttctcagcc tactagtata agaagccgcc
     6601 actcgggatg caaaaaatcc aactgcaact acggacaaga aggcaagaaa gggcattccc
     6661 atcatcatgc ttgccggctg ccacaactca tctaaattag tttcttgact acctagcaaa
     6721 tccgtaattt tcgagatata ggtcggcact tccaactcta gatagaccga aaagcaagta
     6781 aagagaatgg ctagtaaaat catcccccat tcttttctac taattctttt ggttaatttc
     6841 tttattctct cctcctattc ccttgatatt ttgcctgtag ttgaccgaga actttctcaa
     6901 aaatcagtaa ttcatcttca tcaatgtctt ccatcaactg cttgtctatg cgttcaaaaa
     6961 aagccttaac ctgttgcatc tgagaacgtg ctttgtccgt cagacgaaca aacttagccc
     7021 gcttatcaac aggactcgcc tccaattcca ccaaaccatt ttgcactata cgcttaacca
     7081 gattactagc aacagacttg gtaatattga gttcctgctc gatatcttta atcaagacca
     7141 agtcttgatt tttctagcga ttatccaaaa aacgcacaac ctgaccttgc ggcccaccca
     7201 taaattcaat gccgcaacgt ttggcttcct tttgcaccat caggtaaatt tgatgaccaa
     7261 aacgcttaaa gactaacatc ggtttatcca taatctcccc cttctaaata aaaatagttc
     7321 tctggagaat aattaaattt ctatgagaac tattttcttg attaaaaaaa tcccaagtga
     7381 ttttctcact taggatcatg ttctataggt taaattaaaa cccatctacg ttcgtataaa
     7441 tcttttggac gtcttcgtcg tcttcaagaa cgctgtaaag tttttcaaag gtttcaaggt
     7501 cttcgcctga caattccact tctgactgag gaatcatttc caattcagtc acttggaatt
     7561 cttcaatacc agactcacgg agagcaacga tagccttgtg aaggtcagtt ggcgctgtgt
     7621 aaactgtgat tgtaccttct tgtgcttcta cgtcatccac atccacatcc gcttcgagca
     7681 attgctcaaa gactgcgtcc gcatcttcac ctccaaatac aataacacct ttgttgtcaa
     7741 aaaggtaaga aacagaacct gaagcgccca tgtttccgcc gtttttacca aaggctgcac
     7801 ggacattggc tgctgtacgg ttaacgttag aagtcaaagt atccacaatt agcatagagc
     7861 catttggccc aaaaccttcg taacgtcctt ctgtaaaggt ttcgtctgtg tttcctttgg
     7921 ctttatcaat cgctttatcg ataatgtgtt ttggcacttg ggcttgttta gcacggtcga
     7981 taacgaattt caaagctgag tttgattctg gatctggatc acctttttta gctgctacat
     8041 agatttctac accaaatttt gcatatactt tagagttagc tccatcttta gccgttttct
     8101 tggctacgat attggcccat ttacgtccca ttaggaatct ccttttttca cattttaatc
     8161 tttcttatta taacacaagt ttttttgatt ttcactagag gaaatggatt ttattagcaa
     8221 atcaagctag gatagcactt tacctgctaa gatggtcttg cctttctatc tttatcaaca
     8281 gacactcatc cacattcaaa aaacaaacta gaccattatc tgcaaataga aagtttcagc
     8341 caagtttgac aaagtcagct caaattactg tttgaagttt gtagatataa gcgacaaaaa
     8401 caatcatact gcaccttttg ttgacagtct actccagaca tatcatagtt caagtaaata
     8461 ctttgaaatt caacagttct tataggcgct attgtattct aagaaatcaa tagaagagtt
     8521 tctaagcaaa cctctaatac tcaataaaaa tcaaaaagca aactagaaag ctagccgcag
     8581 gttgctcaaa acactgtttt gaggttgcgg ataggactga cctggtttga agagattttc
     8641 gaagagtata atttacgtgt tcccaagatg gagaaattag actagtacac tggcacttct
     8701 aaaccattgc tagcaattga tttgttcata tttaatttca ttttttccat aaatgggtat
     8761 tagatataaa cagcaaaata tttccgatac gtgtcattct tgaatttcca atcatctaaa
     8821 acaagtaaag gataatcaat cccctgtata tcaaggaatt ggctaccctt tttacttttt
     8881 tacacattct gtttgataga ttcattttaa catcacgagc atactccaat ggaaatcgct
     8941 aggcaagaga taaactttca gatattcgca gagagctcat cgcctctttt tgtcgcaaac
     9001 attcttctct cctaatcatt ttctacctta tcctctacct gaggatagag agttgttccc
     9061 caaatagaaa tcgtccgctt acgcactagt ggcaaatcgg ttttttcata aaccgtacgc
     9121 caccattccc aggcaagccc ggtacactct ctaattttga cagagagatt acgaacattc
     9181 ccttttaaag gaatactagt ggtaaagtga gccgtcaaat cctgcccatt tctgtcccaa
     9241 gccttaggag tcaagacttc cttaccttga tgatcatagg ataattcatc ccaagtaata
     9301 taatattggg caacataggc accactatga tccagcagta aatctccgtt tctgtaagct
     9361 gtaaccttag tctcaacata gtctgtacta ttttgaaagg tcgcaactac attgtcacgt
     9421 aaaaaagaag ttgtatagga aatcggcaag cctggatgat ctgctgtaaa gcgactgcct
     9481 tcttgaatca agtcctctac catatccacc ttgcctgtta caactcgggc acccgaactt
     9541 gggtcgcccc ctaaaataac cgccttcact tctgtattgt ccaaaatctg cttccactct
     9601 gtctgaggag ctaccttgac tccttttatc aaagcttcaa aagcagcctc tacttcatca
     9661 ctcttactcg tggtttccaa cttgagatag acttggcgcc cataagcaac actcgaaata
     9721 tagaccaaag gacgctctgc agaaattcct ctctgtttta aatcctctac cgttacagta
     9781 tcttgaaaca catctcctgg atttttaaca gcatctacgc tgactgtata ataaatctgc
     9841 ttaaaattaa caatctgaat ctgcttttcg cctgaatgga cagagttaaa atcaatatca
     9901 agagaattcc ctgtcttttc aaagtcagaa ccaaacttga ccttgagttg ttccatgctg
     9961 tgagccgtga ttttttcata ctgcattcta gctgggacat tattgacctg accataatct
    10021 tgatgccact tagccaacaa atcgtttacc gctccgcgaa cacttgaatt gctggggtct
    10081 tccacttgga gaaagctatc gctacttgcc aaaccaggca aatcaatact ataagtcatc
    10141 ggagcacgat cgaccgcaag aagagtggga ttattctcta acaaggtctc atccactacg
    10201 agaagtgctc caggatagag gcgactgtcg ttggtagctg ttacagaaat atcacttgta
    10261 tttgtcgaca agctccgctt ctttctttcg ataacaacaa actcatcggg tagctgatta
    10321 ccctctttga tgaaacgatt ttcaatactt tctccctgat gggtcaagag tttcttttta
    10381 tcgtaattca tagctagtat aaagtcattt actgctttat ttgccatctt atacctccta
    10441 ataagttcct ggattgagtt gcataaactc agacttgttc agcgaaatca gccgtggttg
    10501 gactaagtga tccaaaattt cctcgtacaa ttcttctgag acattgcgtc gccgtctggc
    10561 tagataagaa gtcggaataa ccgtattatc caacataaat accttatcta agtcaatcaa
    10621 ggttggtctt gtaaaaggat tacgagctag atccggctct tctatcataa agttcttgac
    10681 caaacgtctg gtcaagagag ctggtttgaa ggtctgattt ttaaccaact ctttgttttt
    10741 agtcatgctg ttgtcaatac agatatacat atgattcttc acagccaaat cgctactaat
    10801 agtcggaaaa ggcaaataaa gagctacaac atctcctctc ttaatcaagc aagagcaccc
    10861 ccttttctcc taatgtaaca tagacaggat tgaccaagtc ttctgattga ctcagaattt
    10921 ccaaagtttg agtttggcgc gctgtcaatt tagtagcatc ttgtctcttc aatacaaaat
    10981 gcttgtcgcc aataaccttg acaatataat ccttctccaa agctgactgg taaatccaca
    11041 tcagatgttg tctgtcctga gaactcaaga gagaaggatt ttcaagcctc ccgatagtct
    11101 gataaaaatc aaaaacagga gctaactcct gccaatctga ttggctagtt gtcaaggcta
    11161 gaaaaagggc tttgcgagct gatacttctt ggttagcctt gagagttact ttcccctcca
    11221 agttttttag aaatcgggaa actccagaaa gcaaattttt ctctaactgc gagaaataaa
    11281 aacctttcgt tcccagacat aagtctttca tgtcgctttc tctagcaaat aagagctcaa
    11341 acatttgata gtaaaagaaa aatatctggc actgggtcgc gctcatcttt tccttatcgg
    11401 cttctttttt taaccagagc aagggcgaca ggtagctgga ttgagacatt tcctctacct
    11461 cctactcttt tttaactgga gcatctgcac tagctgccac ttcttttgac tggatacttt
    11521 cccactggtt aatctcctct gagataagac cttcgcatgt cttgacaaat agggcaaaag
    11581 ccttggtctt tcctgcatat ttctccgttt ggcattgata gaggaatttt tctttctcca
    11641 ggagttgcgc agttttttgg taagaaatcc aattttcctt tgcattatac aaattgataa
    11701 tcccctcaca cagcaagccg agactggata aggcaaccga aatcaaacgg tagcgatcac
    11761 ctggcatagg aatagcacaa aagacagcta tgaggaaacc tgccacgatt tctgttattt
    11821 ttaatacctt atagcgccta cgatgttgaa cgcttttctt taaaaaatga gctatctgta
    11881 cgtctaatcg ctctgtcagg tacatttctt ctggcgtcat attcgtaact cctttcattt
    11941 actttgataa ccatagtata acatatttga atatcaaagt atagtaaaaa ctggactata
    12001 acttattaga ctccctgcga aacaaaatat ggtatagtag ttctatgaat tatgaagcaa
    12061 gtaaacaatt aactgataca cgatttaagc gtcttgttgg tgttcagcgt accacttttg
    12121 aagagatgtt agctgtatta aaaacagctt atcaaaaaag tcggacaagc tggtaaaatc
    12181 ttggctgaca gtaattatca agggctcatg aagatatatc ctcaagcaca aactccacgt
    12241 aaatccagca aactcaagcc actaacagtt gaagataaag tctataacca tgcgctatct
    12301 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgttttca
    12361 acaacctgtc gaaatcatcg taaacgcttc ggattacgaa tgaatttgag tgctggtatt
    12421 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatatatat gcacagacat
    12481 atgaaatctt tttgatacag ccttcttttt acatagagag acagcagtta tgatatattt
    12541 atccattcac aacatcttct gtaccaatac tacaatattg gtacattttg attacacaat
    12601 cgtttcaaaa aatatgatgg aagcctgagt attttatagt aattcccttc ctatcaacta
    12661 gatcaaccag ttgagtgttg aatgttgaaa caccctagcc tcagcaaagg actatgatca
    12721 gcatttccta gccaatagga cgctccaaat cacctattct aagtccatca ctagcttggt
    12781 cagtaagata cagactgcaa ggcaagactg acgtggttga tgagattttg gaagagtatt
    12841 acaatcatag attcaccagt agacttgacc gcagatttaa ctatagtaga ttgaaactag
    12901 aatagtacac ttctgcttct aaaacattgt tagaaatcga tttgactgtc ctgatcaatt
    12961 tgtcctgttc tcgtttcatt ttactatact aaaaaaccac ctgaaacaaa tcaagtgact
    13021 agaggataaa aacaaagact gggcaaaaag ttttgaaaag caaaaaacgc ataatatcag
    13081 gtcttgaaaa accttgatac tatacgtttg attgtgggaa gattgacttc attttctcct
    13141 gaaattgaag ttttttctag tttccagata tagacgaatt caatatagcc atctcgctac
    13201 aattatttta accatcgaga aaatttttca gaatttcaaa aatttcattt tgatccaatg
    13261 aaatgtcatc attttgaaga atagttgact tcacaggctc taattctatc acaaagcggt
    13321 gcatgtctgc ttttaccgca ctacttgata ttatagccga taacttttca tctcctagca
    13381 aggaagctgt caaacgggta atgtcattca aatgcttttt aatggactta cttaaatctt
    13441 gagcacctgt agcagagcgc tctctcatat ccagccaagc tttagcctta aaaataatt
//
LOCUS       DAVLRT010000048        13278 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLRT010000048 DAVLRT010000000
VERSION     DAVLRT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.048"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            204..380
                     /locus_tag="VXM85_001426"
                     /pseudo
     CDS             204..380
                     /locus_tag="VXM85_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            563..769
                     /locus_tag="VXM85_001427"
     CDS             563..769
                     /locus_tag="VXM85_001427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1345987.1"
                     /translation="MREYRTYEEIAADFGIHESNFIRRSQWVEVTLVQSGFTVSRTPL
                     SSEDTVMIDATEVKINRPKKTISE"
     gene            795..1184
                     /locus_tag="VXM85_001428"
     CDS             795..1184
                     /locus_tag="VXM85_001428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1345988.1"
                     /translation="MKAQAIVTSQGRIVSLDITVNYCHDMKLFKMSCRNIGQAGKILA
                     DSGYQGLMKIYPQAQTPRKSSKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKIIS
                     TTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(1236..1598)
                     /locus_tag="VXM85_001429"
     CDS             complement(1236..1598)
                     /locus_tag="VXM85_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1345989.1"
                     /translation="MPKTKTLAKLADVILWSFDFANDHAHAFFMDNVEWSHADSYFFS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1952..3832)
                     /locus_tag="VXM85_001430"
     CDS             complement(1952..3832)
                     /locus_tag="VXM85_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1345990.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVTIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RHAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3826..4695)
                     /locus_tag="VXM85_001431"
     CDS             complement(3826..4695)
                     /locus_tag="VXM85_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1345991.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILYKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQTCTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4779..7424)
                     /locus_tag="VXM85_001432"
     CDS             complement(4779..7424)
                     /locus_tag="VXM85_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1345992.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKVLHPTEGYKKMAALILPSYRVEDL
                     DGHPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7515..8795)
                     /locus_tag="VXM85_001433"
     CDS             complement(7515..8795)
                     /locus_tag="VXM85_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1345993.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFVELDLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8966..11050
                     /locus_tag="VXM85_001434"
     CDS             8966..11050
                     /locus_tag="VXM85_001434"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1345994.1"
                     /translation="MKPLLETIDTRLGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTGQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDVIETGEANRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLKTASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(11090..12769)
                     /locus_tag="VXM85_001435"
     CDS             complement(11090..12769)
                     /locus_tag="VXM85_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1345995.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPAYG
                     NGGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGNCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3728 a         3132 c         2686 g         3732 t
ORIGIN      
        1 aaaaagtttt attcaatcat tatctagtag aatagagtag tacattttat aaattgtcat
       61 ctctccataa acacagtata tcatttttaa attttaagtc aaggaaaatt aactgagtaa
      121 aaactctcga aattcaattc gctatattat cactcaactt gaacaaatac cagaaccaca
      181 aaaaacagat gtaaaggaaa aatatggact taaaatttga aggggttgat ttagaataca
      241 aaaaagctaa aaacaatctt ccagagtctt tctgggaaac ctactcagca tttgcaaata
      301 caaatggcgg gaaaataatt ctcgggatag atgaaaaaaa cattgacacc taccaaagag
      361 tcaatagact ccctgcgaaa caaaattatg aagcaagtaa acaactaacc gatgcacgat
      421 ttaagcgtct tgttggtgtt caacgcacga cttttgaaga gatgttagct gtattaaaaa
      481 cagcttatca acttaaacac gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc
      541 ttcttatgcc cactcttcaa tagtgcgaga atatcgaact tatgaagaaa ttgcggctga
      601 ttttggtatt cacgaaagca actttatccg tcggagccaa tgggttgaag taactcttgt
      661 tcaaagtggt tttacggttt caagaactcc tctcagttct gaggacacgg taatgattga
      721 tgcgacggaa gtaaaaatca atcgccctaa aaaaacaatt agcgaatgat tctggtaaaa
      781 agaaatttca cgctatgaag gctcaagcga ttgtcacaag tcaagggaga attgtttctt
      841 tggatatcac tgtgaactat tgtcatgata tgaagttgtt caaaatgagt tgcagaaata
      901 tcggacaagc tggtaaaatc ttggctgaca gtggttatca agggctcatg aagatatatc
      961 ctcaagcaca aactccacgt aaatccagca aactcaagcc gctaacagtt gaagataaag
     1021 cctgtaacca tgcgctatcc aaggagagaa gcaaggttga gaacatcttt gccaaagtaa
     1081 aaacgtttaa aataatttca acaacctatc gaaatcatcg taaacgcttc ggattacgaa
     1141 tgaatttgag tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta
     1201 ttgaaatact aagcctacac agactgattt caattttacc attcttcata gtcaaaacca
     1261 tcataatctg gatattgttc tggcgacgtt ccaaccgaac gtacgagata agcttcttcg
     1321 tcctctgtct cgatttctct cagcacttgg cattcaaaac gccattcatc accgaagtcg
     1381 aaaataaact taaatttttg tttgacactt aggctatcca gatagacatt ttctgtgtaa
     1441 cgttcttcaa catcgtcact aacaaagcta aaaaagtaag aatctgcatg actccactca
     1501 acattatcca tgaaaaatgc gtgagcatga tcatttgcaa aatcaaaact ccataaaatc
     1561 acatctgcta gtttcgctaa cgtttttgtt tttggaatct atatatgtcg gtagcaacct
     1621 tttttaaatt tgacggaaat gacccaagct tctatttctt gttttactaa ttttaagtgt
     1681 agcataatac ctccaattgc tcatcgatag aattgctctt aggaaacatt cactttctgc
     1741 tattttatca taagtttgtc taactgtagc atttgaaacc agataattct tctgtttcac
     1801 gcgaaacacc ctgcgccaag caaggtgttc ttgtttttat actcaatgaa aatcaaagag
     1861 caaactagga agctagctgc aggctgctca aaacactgtt ttgaggttgt agatggaagc
     1921 tgacgtggtt tgaagagatt ttcgaagagt attaagtcgt ataaatcgtc gacgctgtcg
     1981 caatggtatg ccactggtta gctgtagttg ctgcgaagtc cttgtctgcg tagaagtcag
     2041 taaatggtag gatttcaact tccagctcgt cgatgcggtc aagctgacca gccagataaa
     2101 cctcgatacg gctttctgct cgtttgatgc gttgcaagag tccgcccata cggatgtcaa
     2161 ctgtatccaa accaaagacc ttgttttctt tcagccattg gtggctaaag agggcatgga
     2221 agtcttcaat ttggcttcta agttctggta attcttgtct ggcgatttgt tgtaaacttt
     2281 ctttatcatc cgcttggtag gcatgacgaa tgcgtcgtcc cacatctact ttgctactta
     2341 aaatagcatt caactgggcc tgagtttcaa agagataagc atagtttcca gctttttctt
     2401 taatgttagc aagcgtctca gcagcctgag cgaagtgcgg tttgtcctgt tcaggtgtca
     2461 tgtgttgatc aagaatcgga caaagaatat cctgataaaa aacatagcgg ttgggattga
     2521 taccgctgag attgcctggt aggtctggta agaggttggc aagatcaatc tgcataaaat
     2581 cctcaaccgt tagaccagta ttggtcttga agtgcgcaga caaactatct aggtcattgc
     2641 gatagctgag ttctgcccag atttgcaagc ttggtaggat agagaactgg gcagtttcac
     2701 caccattgtc tccccaacca gttacgatga cttctttaat ctgattggca cggcaggctt
     2761 tattagcctc gatagtcact agacggctaa aatggttgtg aggtgtaaag ccaatccact
     2821 tccaagctcc ccctgcaaat gcaaggtcat ggctaatctt gtgatgattg cggaaattac
     2881 ggttgtattt ttcctcgcta tcctgataat aatcccagta aaccagagtc acacggtctt
     2941 tgagacggtc taggtagaca cgagtttcct ctggaatttc cacatcacgg tcgtactggc
     3001 catccgctga catgagtttg aagaacatat cactccacat ctggcagtgg aaaccatatt
     3061 tgtcagcaat atccagcacg cgctccaagt gttggcacat gaggagacta cgatccacaa
     3121 caccgttcaa aatcaggtag cgtcccaaac caaccaagtg ggcttcgtcc atcccgatat
     3181 tgaccttgcg agtcttcagt ttagacaacg tggcaaacat gccatcaatc aggtcataaa
     3241 ccttttcttc gccaatgaga agaatgtcct ctacatcacg gagctcctgg acttccttga
     3301 caccccattt gacaaaggcc gacaagtggg ccaaggtctg gatgcatggt acaaaggtca
     3361 tgtcaaactg ttgggcatag gcttcgattt cctgcaactc ctctgctgaa taagctccac
     3421 ggaaatagcc aaagtaaggc tgtccttcaa tctggtaagt gtcttccatg taaagctcaa
     3481 aggttgagta gcccatgaga gccaagacct caatcatctg cttggcagaa gccacattca
     3541 gcaccgcatt tcgagaacag tcaaccatgt aagccaaatc ttcgtaagct gcttgttcct
     3601 caatctctac tttatcagct tctgctagaa ctgttaccaa caaggataag gcacgataaa
     3661 gttggtgagg tttgcggtag gtcaattgat agtgaccttc ctcaccctcg atagagatag
     3721 aggcttggtc agactgagtg acagccactt ccacatctgg tagagaaata tgacctttta
     3781 aaacctcaat agcttgcgtt tgtttgggac taagtcctgt aaatcttacc attgcttttc
     3841 ctcctgtagc cagttgacaa gggcaccgta gagattggca tctgcgtgat aggtgcaggt
     3901 ctggataact ggtgcgaccg tgtattcttc gtaggcatcg acaaagtctt caacagcctt
     3961 cttaacacct tggataaaat ctggattttg actgatagag ccacccagac tgatgacatc
     4021 tggatcgatc agatactgga tattgagcaa gccttgcgtc agattgcggt tcatgcgctc
     4081 aatggcttct tgacaaagag cattaccagc tgcggcctct tggtaaatct tgcgaccgtc
     4141 ccaatcagta tgaccagatt tttcaatcac gtatcgtacc atattcccag ttgatgctag
     4201 ttgcgaccag ttattaagtt tttcagcagg ggcaagggtt gtcatgtagc caaattctcc
     4261 acccagaccg tggcgacctc gatgaagtct accattgata atcatggctc cgccaatccc
     4321 tgtcccaatc acgacacagg ctgcattttc aagctctgga tgagctagta gttcactgag
     4381 tccaacgcag ttggcatcat tttctaaatg gacaggtagc tgataagagc taagcgcctc
     4441 ataccaagaa aagccatgga tgtagggcac cgcactgaag ccatcaatca cacctgtctc
     4501 ttgattgacc gcacctggaa cgctcatagc aatcccactg taatcctgtt ctgacaagcg
     4561 ttgatctagc cacgctagta aatcctccaa gttttcaggc gttgaaatac ttgtcttata
     4621 cagtattttc ccatcaggag tcagactggc aaacttaatc ccagtccctc cgatatcaat
     4681 cgttgcaatg gtcattgttt ttaccataaa aaggggcgaa tcagcagtta gttcttactt
     4741 tttcgcccct cctttctttt gatgtttact ttttgaaatt aaatttcttc ttttttgatg
     4801 aattctgtac gaatctcttg tggagccaat agtcctgaat gaactggata tggtcgctca
     4861 agtaagtcaa gaatggtttg ttgatgttct gatatgcgca cattttcttg actcatatta
     4921 tagtaacgga ggacatagcc ttcttcattt tccgccactt taaaggctgt tggacaaact
     4981 tgcggtatgc tgagaacaga atggctcaag aggctaccag tcgcaaccac gcttccttcc
     5041 tgtctagcaa gctgaaggct ggtaaacggt gtctgcaagg ctttggcacg acgataggct
     5101 gagaagcgtt cttgggcttg gtggcattca agtgcaaact cgacttcaaa ctcacgcaag
     5161 cattgtgctt ctggcgttgg gaagtagccc cagtcaccta gctcacctga tgcacgcaaa
     5221 atggtcacgg caatggtgtt atccccaagg atttcgtatt cattcaatcc cttattggat
     5281 acagtcaccc ctttttcatc gtcatacaga ctgacaaaag cttgttggtg ttgaggattt
     5341 tcaggatttt cccatgaagc agctggtttg tttggtcgtg tcaccacctc atagatactt
     5401 tcagaatcat tgcttggacg cgtgttatga gtcttgacca agagacggat acggtgatcc
     5461 ttggcagtgt tagtaaagcg agtcttgaag cggatttgtg gattgtcaac gaagacagtc
     5521 aactcagttt ccagaggaat gtttgtcaat tcttctgacc gtccagcctc acgcttcata
     5581 aactcgatga taccttgttg ctcttcttct agcttttcat ccgcgctgac aggcacggtc
     5641 aattcatgtt tgagcaagat tttagcatag caagctgtgt tttccaagac ctcgtggccc
     5701 ttaagttctg caaagattgg ctctgttcct tttggttgga aatagatata ctcgtttccg
     5761 atgtccccac ggtcttcaaa gcggataaag tcttcatagg cttcgtgagt tgtcttgtca
     5821 tagactgtga tgttgtcatc cacactcacc gttacgaatg gtgtatcaat cactccgttt
     5881 tggtaaatac cctcacggtg ttcttgtttt ccttccagca attggaaggt tgtccaagaa
     5941 agcggcgcta ggtgaactgg aatggtcacg cgcacttgac gagcaatacg agcttggcgg
     6001 aacttgtctt ttggtaaatc atactcaaaa ttagctccga ggtcttcgat tgtagcctct
     6061 acaggatgac catccaagtc ctccacacgg taacttggca agataagagc agccatcttt
     6121 ttgtagcctt ctgttgggtg caataccttg aaatcacaag tcgccacatc aatcactgtg
     6181 ctgacagtat cgaccttatc atgcaagcct gtgttaatga cagtaaagag atagtcactt
     6241 tgagccttat ccgtagcaat tttacccttc cactcgttga gcaagttact tttaacaaag
     6301 tttcctactt ggttgacctt ggcaaaacgc gtttccatct cgcggtgaac ttcgtccacg
     6361 ctacagccac agatactatc atgtggcgca ttctgcaaaa gtgttttcca agcataggtc
     6421 aactggtcct tgtggttgtg tccaccagtg ataatagtca agggttctac cacttgctct
     6481 aggaggttgc tattttcttg gaaggcttgt tttaggtaaa tgcgggatga agaagtgttg
     6541 gcaagtgtgt accagccatc tgtttcctga ctggtcaact cgcctgtaac agttgataag
     6601 tgttcaggaa gcgcaccttc tacagcttga acatattcat caaaagaact atgaacaaag
     6661 attacatccg ggaagagttc atttgccaca cgaatggctt cgctcagatt tttctgtaca
     6721 ggctggtggt cacagccgtt catcatcaac cattggttgg tcgaagcgta ggcacgcaca
     6781 tctgacaatt tttgtttcca gaaggtcaag gcctcatctt tgtcaactgg gatttcattc
     6841 ccattactgt accagttggc aaagagaata cctaaaacac gactaccatc cacaccctgc
     6901 cagtacattt ctgaaaactg ggatgtaaac tgctcatctt caaggacttg gttgtcaaat
     6961 ccaatcggct tcacaccacg accaaaggcc gccacgtgaa tgcctgattt ttgaagaatt
     7021 tgaggcgctt gtcccatatt tccaaaggta tctggaaagt agccaatctg ggttgattta
     7081 ccccatttgg cagcttcttg ttgaccaatc aaggtattgc ggacattggc ttcactggag
     7141 atcaagtagt catcctgcaa gatgtaaaag ggaccaattt taagtttgcc ctcgtcaatg
     7201 tagcgttgga ccttgtcgcg attttcaggg cgaatttgta agtagtcatc aaggacaata
     7261 gtttgtccat ccaagtggaa actcttgaac tcagggtcat tttcaaagag atcaaagaga
     7321 ttgtcaaaca attccaccaa ctgcatacga tggctttcaa aaggcaagta ccactcacgg
     7381 tcccagtgac tatgtgagat aatatgtaca acaacatttt ccatgaagta aaacctcatt
     7441 ctaaatttaa attttaacgt tttaaatgta aacttgtgat tctgataaga atcggttgag
     7501 ctaaagcgag ccccttagcg aatatccaag taatccaaga ccaactcaca gaacatcatg
     7561 ttagcccagg agaaccattc acgagagtag agggtcggat catctacgtg gaagctttcg
     7621 tgcatgacac ctgtaccacc atcgcaggca accagctgat ccagcaagaa ttttttctct
     7681 gccttatctc ttgttgtcaa tccttggata gaaagggcga ttggccagat atagcgatag
     7741 aaggtatgag aacttccgag accgctagcg tattctcctt ggtagaagta tggattttca
     7801 gagctcagaa tggtacggcg agtggcttga tacacttcat catcgatcga acagtagccc
     7861 agatagggcg cagctagtag acttggtaca tttggatcat ccatgatgct ggcatttcct
     7921 aggccatcta cttcaaaagc gtaaatcttt tcgcccttgc tgttggtggt gtaagcgtag
     7981 ttttcgattc cttcttggat ttcatcctga agacgcttgg catcagcaat aacactctgg
     8041 ctatcagcta ggtctaattc gacaaagatt tcttgcacat aacccaagac tactacggca
     8101 aacatattag acggaatcaa gtaactatac tgacagcaat cgtcactcgg acgaaaagct
     8161 gaccaagtca tacctgtcac tgcaaagtca ggtccaaagc catcatttac caaggtgtct
     8221 tccttacggt ccgtatctcg gacaaaacga taaggagagt tcttgtggtc ttgttcaact
     8281 gtccatagat ggagaatttc cttagtcgct gcgacaaaag tctcatcaaa ctgaccagtc
     8341 tcgccagtct ctttccagag gagataagcc aactgcaaag gatagcaaag tgaatccacc
     8401 tcatacttgc gctcccagat ccagccgtta aggtctgtgt ggtcagtctc gtggtgccct
     8461 ttccagttct tctcaatgtt gaaggagtta gcatagggat ccttgagtac caaggtcatc
     8521 tgacgtttga ccaaacctgc aatggtctga cgcaggaggg catctctttt agctacatga
     8581 aggtagggtc tgagttgggc tgtcgaatct cgaagccaca tggcaggaat atccccagtc
     8641 aagacaaaag ttgaaccatc ttctaagatt tcaaccgtat tgtccaaggt atcggtgtag
     8701 caacgctcga aaacatccac ccactctgga tggtccttag cccgctctgc tacttcatct
     8761 agccattctc taacaatttc tttcgaataa atcatcgtgc aattccctct ttcaaacaag
     8821 tttcattttc agtatatagc ttttgagaca gaaaatacat acacaaatga tagtcatttt
     8881 tctacaaaat agttatcttt gaaactcctt ttctaaaggc cctctttttc tgatataatg
     8941 tagaaaaaca gctaaaggaa agactatgaa accactactt gaaaccatcg atacccgctt
     9001 gggaactacc aacaagcatg ccttttctcg gggaaatacc ctgccataca caggcgtgcc
     9061 ttttgggatg aattactttg tgccccagac cagtgaccag gatggatctt ggttcttcga
     9121 tccgcatctg cctatctttc aggggattcg attaactcac cagcccagtc cttggattgg
     9181 ggactactct tggctccttc tgacacctgt cacaggtcag cttgggggag acagtctctt
     9241 tcaccgccaa tcttcctatg atagggataa ggcctctttc caacctcatt atctaaagat
     9301 tttctccctg cgctatcaaa ttgaaaccca gctcacaccg acttgctacg gtgcttctat
     9361 tcgtttgaag caaaagcaag gcaaagccct ctccctctat cttcacgcag cagatgaact
     9421 gacagtagag caagtagata agcgaactct tgccctgcga caagaaggta aaactgaaac
     9481 caacaaaaat tcgctaacga tgttcactgc cctgcaaatg aacacggata ttcttgctat
     9541 cagccaagaa gctggagact ggcgaattga cttagcaagt agtcaaaccg agatgcaact
     9601 agccacttct ttcatctctc cttctcaagc tctaatcaat ctacctcaag aagattttga
     9661 tagctgtaaa gcaagtgccc aagcggattg ggaaaatctc ctccatcgtt ttgatgttat
     9721 agagacagga gaggctaacc gaaccttctt tgaccactgt ctctacagac tcttcctctt
     9781 cccacagact ttttatgagg ttgatgagtc agggcaagcc atccacatgg atctggctac
     9841 tggtactgtc aagcctggtg tcctctttag caacaatggt ttctgggata ccttccgcac
     9901 caccttcccc ctctttgccc ttatcatacc ggagcactat caacgctttt tagaaggttt
     9961 cctcaatagc taccgcgata ctggtttcct tccaaaatgg ctggctccag atgaacgggg
    10021 tatgatgcca ggtacacttt tagacggtat tatcgcagat agcgcctgca aggacatggc
    10081 ccccgaccta gaaggagaac tcttccaagc catgctcaaa acagccagca aagccgaccc
    10141 tctcggcatc aatggccgcc acggactagc ccaataccaa gaactaggtt acctctctac
    10201 cgaccaccac gaaagtgtta gccatactct agactatgcc tatagtgact tttgtatcgc
    10261 caactgtgcc aaaaaactaa aaaacataga aatcgctgaa acctacaagg ctgcttcaca
    10321 aaattaccgc cagctatttg acgctgagac aggttacatg cgagcacgag acaatcaagg
    10381 aaactttcac cctgacttct ctccttatag ttgggggcga gactacgctg aatgctctgc
    10441 cattcaagct actttaggcg ttctccacga catccctggc ttaatccaac tgatgggtgg
    10501 aaaagaaacc tttagcaact atcttttgaa agcctgtcaa gatgctcccc tctttgaaac
    10561 aacaggctat ggttacgaaa ttcacgaaat gagcgagatg gctactgctc cttttggaca
    10621 aatagccatt tccaaccagc ctagtttcca cattccttat ctcttccgct acagcgatta
    10681 ccctgactac actgcccttc ttatcaagac gctccgtcag aaagcttttc acccaagttg
    10741 ggaagcctat cctggagatg aagacaatgg tagtctctct gcttggtaca tctggtcagc
    10801 tctcggattt tatccaacct gtccaggaaa accaagctat gatctcggaa ttcctctctt
    10861 tgaccatcta cgagtctacc tagctaaaga aaataaatgg ctagatattc atactgagca
    10921 aaaccacagc cattttaact ttgtcaaaga atgccgactg gacaaaacac tcgtatcaac
    10981 tattcaacac caagacctcc taaaagctga acaactaact ttcaccctca gctggctacc
    11041 aaatcactaa taaccaaaag aacctttgca tcgcgcaaag gttcttcttt tatgaaactt
    11101 ggacttgaag ttgatccaca agctgaccat ccaccgttag gttcagatag aaatccaagg
    11161 ttttatctcc tgcgaaatac agggttggta gatgcaaatc ttttttgctt tcccctgctt
    11221 ggaattgaag gacttgaatc tcatcctgat aggcgacacc atggacacct gtccccggtt
    11281 gaatcgaaat cttagcctct aaagaggagc tactttcact acgttcaatc ctaaaatgaa
    11341 gagtctctcc ctttgccaca gctaggcttt tttctgcaaa tgctagcccc tgaacagcta
    11401 ctgtttgtga taacctcgga gttttataga gtgaaatctt ggtcaacaaa ggcaaagcct
    11461 gtgattctgt aatgactaca cgtatcttct gtgcttcaac aactgctcct cgcaagaggc
    11521 gcttatgacc aactgtgaag cccctgccaa attcctgcca gacaccatcc acctctactt
    11581 gcacatggaa agcagaaata cgttggccta acttcaaatc ttctctcaac tcaattacat
    11641 caaaagtttt aggagaacct aagtcgagtt ctaactggat gggcaagtct gcatcgcttg
    11701 cccaagagct ggtctcaagg ccgtctgtca aatggcgaca agcaaagtct gcggaaagag
    11761 ctggaccaga tacctcagct cccagagcca aatcttcttt atagagctca ttgcgatagg
    11821 tcgcaaattc ataaagtcgt tcaatatcct ttgcatcaaa gagcccagct tgattcggcg
    11881 gaatattaag caagagtgga gttcctcgcc ctactgagtg aaagtagatt tcgaccaact
    11941 cctcgagaga cttaggatcc tgatcctcat ggtagaacca gcctggacgg atggaaacat
    12001 ctgcctctcc gattgaaaaa atcgtgcccg agggatcccc gtgctgaaga tagtccagct
    12061 ctgcttctgt tcctagttta tcaggattca ccttttgcca cagtggatca cctgcatacc
    12121 ctcgttcatt gccaatccat cggatactgg tgccttctgt tgaaaaaatc aagcaattgc
    12181 cctgcaggtc acgaatggtt tcaaaccatt tttcaaattc ataattaacc ttttgcgcgc
    12241 cctctcctct ggcaccatcc atccaaacct cagcaaattt ccctccattt ccataggcag
    12301 gatttgataa gatttccttc agctgagcca gataataggc attgtagtcc gcttctcggt
    12361 ccacatgata gaggggacta tgggcatccc acggtgacag gtagaccccc atatccatat
    12421 caaactctgt ggcagcttgg gatacttcaa ggagcaagtc gccctttcct ctcctccaag
    12481 gactgacctt aaccgaataa tctgtgtgag ctgtcggata aaggacaaag ccatcgtggt
    12541 gcttgaccac caaaatcaac tttttgaagc ccgtttcctt gagcacacga acccactcac
    12601 gcgcatccaa ctgactcggg ttaaagcgct caggatcctc ctgtccagtc ccccattctt
    12661 ggtcataaaa ggtattagga ccgaagtgga taaaagctgc tagttcatct cccagataag
    12721 ctagctgagt ctgacttggt aacggtccat gcggtttgat tttcttcatc gttatatatc
    12781 tccttcttta gcaacttctc ttgttcacaa ggttatccac aggttgtgga taataaaaaa
    12841 tgatctaacc gccagctctt tccaactcca actggtaggc tcaggatttt tctcctcaat
    12901 attccctctt caaacagctc tgtcatatgg ttctgctaac acctctcaga atgctactct
    12961 ccataattat tctcaggctt ctttcaggtc attgtgtgaa gaccattcac tctccagact
    13021 tttccacaga ttgtgaataa gtgattattc taaactatca tctcgtcgac tataacaaaa
    13081 aataaaaaga gggtcaataa gataattatc tcttcctaaa tactcagaaa agattctata
    13141 atttgtacaa ttgaacaaga aatgcttctc caaaatctat cttcaaaaga atgttgcatc
    13201 tttttatcct ttcacacaaa catttctctt tatacagatc caagtgataa ccagatgata
    13261 attggacact ttttggtt
//
LOCUS       DAVLRT010000049        13148 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLRT010000049 DAVLRT010000000
VERSION     DAVLRT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.049"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            472..765
                     /locus_tag="VXM85_001436"
                     /pseudo
     CDS             472..765
                     /locus_tag="VXM85_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            872..1223
                     /locus_tag="VXM85_001437"
                     /pseudo
     CDS             872..1223
                     /locus_tag="VXM85_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1499..2805
                     /locus_tag="VXM85_001438"
                     /pseudo
     CDS             1499..2805
                     /locus_tag="VXM85_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2845..3855)
                     /gene="tsaD"
                     /locus_tag="VXM85_001439"
     CDS             complement(2845..3855)
                     /gene="tsaD"
                     /locus_tag="VXM85_001439"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1345996.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3845..4282)
                     /gene="rimI"
                     /locus_tag="VXM85_001440"
     CDS             complement(3845..4282)
                     /gene="rimI"
                     /locus_tag="VXM85_001440"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1345997.1"
                     /translation="MIEIKRIQQQPALAQDIYAVMAAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLTVQETLFEAEVLQIAVKGACQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4279..4962)
                     /gene="tsaB"
                     /locus_tag="VXM85_001441"
     CDS             complement(4279..4962)
                     /gene="tsaB"
                     /locus_tag="VXM85_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1345998.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5140..5325
                     /gene="cibA"
                     /locus_tag="VXM85_001442"
     CDS             5140..5325
                     /gene="cibA"
                     /locus_tag="VXM85_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1345999.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5327..5479
                     /gene="cibB"
                     /locus_tag="VXM85_001443"
     CDS             5327..5479
                     /gene="cibB"
                     /locus_tag="VXM85_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1346000.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            6297..6530
                     /locus_tag="VXM85_001444"
     CDS             6297..6530
                     /locus_tag="VXM85_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1346001.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6532..8211
                     /locus_tag="VXM85_001445"
     CDS             6532..8211
                     /locus_tag="VXM85_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1346002.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8406..10319)
                     /gene="mnmG"
                     /locus_tag="VXM85_001446"
     CDS             complement(8406..10319)
                     /gene="mnmG"
                     /locus_tag="VXM85_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1346003.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWASFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10329..10784)
                     /locus_tag="VXM85_001447"
     CDS             complement(10329..10784)
                     /locus_tag="VXM85_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1346004.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(10925..12046)
                     /gene="mnmA"
                     /locus_tag="VXM85_001448"
     CDS             complement(10925..12046)
                     /gene="mnmA"
                     /locus_tag="VXM85_001448"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1346005.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            complement(12447..>13148)
                     /locus_tag="VXM85_001449"
     CDS             complement(12447..>13148)
                     /locus_tag="VXM85_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1346006.1"
                     /translation="PAPKPAPAPKPETPKTGWKQENGMWYFYNTDGSMATGWLQNNGS
                     WYYLNANGAMATGWAKVNGLWYYLNSNGAMATGWLQYNDSWYYLNTNGAMATGWLEYN
                     GSWYYLNANGAMATGWLEYNGSWYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAK
                     VNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKANQWFKVSDKWYYVNGLGALAVN
                     TTVDGYTVNENGEWV"
BASE COUNT         3735 a         3063 c         2597 g         3753 t
ORIGIN      
        1 aatttttata atcatcaata acctttgaat ctctagatac gattatcaaa ataggatata
       61 gagacatcct tctgatctgc tttttacaaa gtccaattat atgcggatct atacctccac
      121 aatgtccatt attataccta actataatat gagccgaaaa cactatatcc ttaatgtctc
      181 catatccatc agggatatta atatttattt ttccacaact atattgcatt gtaaccatct
      241 ccttaaacga cgcattatga tatttgatag agaaattttt atgaataact caataatttt
      301 atagtaaatc atgcttatat ctcaaagata cctattttat cttgtctcga ccttctccaa
      361 agaattgcta taatactatt acaaatccat ctgcactaca cttcaaattt tagcactgta
      421 taaaaacgtt tcaatacact aacttcaaga aaacttccac tattaattga aaaaattgat
      481 agagataaat taaaaatcta tattgaaact catcccgatg cttatttgac tgaaatagct
      541 gctgaattca actgtcctcc aacaactatt cattacgctc taaaggctat gggatatagt
      601 ctaaaaaaga gccgtaccta ctgcgaacaa gacccagaaa aagtaaatcg gttccttaaa
      661 gaattgaatc acttaagcta cctgactcct atttatattt atgagacagg ggttgagacc
      721 tatttttatc tcgaatatga tcgagccttg agcaggcagt tagtctctct ggaagaagat
      781 ataattattt gaattaagat cgagacaacg cacaccagag attgcgatac tgttatagaa
      841 gtactaatgc ccttttttgt ttcaatatac tatggctccg atgacctata aagatacgat
      901 gacgagtgac tttttcgaag cttgcttcca aaaattctta ctacctactt tagatacacc
      961 atcccttatc attatggacc atgcaaggtt tcacagaatg aacatgtgta aggagcaggg
     1021 catagactgt taccacttcc tacctattca cccgagtata atcccattga gaaaatatgg
     1081 gcttacatca aaaacatctc agaataatat tgtcaaatta cgatgctttt cttgaggcac
     1141 ttttgtccta ttcttgtttc agccgactat actccgttat tgggcagcta cggaacagtc
     1201 gatgggacga tggggggaca taaaaaaatc ctccagtttt gttttttata acagtatact
     1261 ggagaattga caatctcggt agatacctcg ttatagcgcg gttacttatt aggcagttac
     1321 aaaacaactg tgaacagaaa acattccaga gtcagacaag actttggaat gctttggctc
     1381 tataatttct gtagtgggta atcccacccc aggaattata gggtcgtttc ttgtagaaaa
     1441 aaagccccat atgacctata atgaaaagcg tctaaccaac tcattagaaa gggttcatat
     1501 ggaacaactt aagaatacca cagatttgct cggattggaa gacaaaaata tcaaaatctt
     1561 gtctgttctg aaataccaaa cccatctagt cgttcaggca aagttggatt cccccgctcc
     1621 tccttgtcct cattgtcaag ggaagatgat caaatacgac ttccagaaag cctctaaaat
     1681 tccgcttctc gactgtcagg gtttacccac ggtactgcat ctcaaaaagc gccgctttca
     1741 gtgcaagaat tgccttaagg tggtcgtttc tcaaacatcc attgtcaaga aaaattgcca
     1801 gatttccaac atggtgagac aaaaaatcgc tcagctcctc cttgaaaagc agtctatgac
     1861 tgagattgcc cacagattgg cggtctcaac ttccaccgtc atccgaaaac tgagggaatt
     1921 taagtttgaa accgattgga ccaagttgcc aaaagttatg agttgggatg agtatagctt
     1981 caaaaagagc aaaatgagct tcattgccca agattttgag tccaaatcca tcctcgcaat
     2041 tttagacggg cgaactcatg cggtgattcg aaaccatttc caacgctatc agagagaggt
     2101 tcgggagctg gtcgaggtca tcaccatgga catgtacagc ccttattatc ggctcgctaa
     2161 gcaactcttt ccaaaggcga agattgttct tgaccgcttc cacattgtcc aacatctgag
     2221 ccgagctatg aaccgagtac gaatccaaat catgaaccaa tttgaccgaa aatccttgga
     2281 gtatcgggcg ctcaagcgct tttggaaccc tcgctttttc gtttctaggc tcgggctaaa
     2341 tcagtccact ggactgattt actacaccag tatagcttca agctctgtca gaaacgattc
     2401 tatcagccca cgtttcgaat gcacttaacc catcgggaag tacgagataa gctgctttct
     2461 tactctgagg gattacaggt tcactacgaa ctctatcaac tcctgctctt tcattttcaa
     2521 gagaagaatg ccgaccattt ctttggattg attgagcaag aactgccaac ggttcatccg
     2581 ctttttcaaa cggtcttttg gactttttta agggatagag ataagattat caacgcactt
     2641 aagctgcctt attccaacgc taaacttgaa gcgaccaata atttgattaa gattatcaag
     2701 cgcaaagcct ttggtttccg gaactttaac aattttaaaa aacggatttt gatgactttg
     2761 aacatcaaaa aagagagtac gaatttcgta ctctccagat tgtagctttt cgcctaccca
     2821 ctacacttga caaagagcca ctctttattc catggtatca aaggcaagac ttggtttggc
     2881 attgaggtcc cagcctgcga agttttcttt gttccactcg ctgacgctgg cataggcaat
     2941 catacctgca ttgtctccgc agagtcgcag aggggggatg ataaccttga catctgtgat
     3001 ttcggctgct aggcgttctc tgagaccttt attggctgcc acaccacctg ccacaaccag
     3061 ggttttaaca ggatatttct ccaaagcctt cttggttttt gccatgagaa tgtccataac
     3121 tgctgcttgg aaggaagcac acaaatcttc tgtagacagg ctttctccct tttgctcggc
     3181 attgtgataa agattgataa aggcagattt caaacctgag aaggagaact ccagattatc
     3241 ttccttaatc atggcacggg ggaaatcata aatatcctgc ccctgatgag ccagctcgtc
     3301 aatctcacga cctgcaggat aggtcaagcc catgacacgg ccgaccttat cataagcctc
     3361 accaaccgca tcatcacggg tttccccaac aatcttataa tctcctgcct ccgaaacata
     3421 aaccaactct gtgtgcccac cgctaaccaa gagggctagc aagggaaact ccaaaggttc
     3481 cacactttga gccgccatga gatggccagc catgtggtta acgggaatca gcggaagtcc
     3541 atgtgcccaa gcaaaggcct tggcagctga caaaccaact agtaaggctc cgaccaagcc
     3601 cggtccatag gtaaccgcca cagctgtcac gtcctcttct gtaatccctg cttctgctag
     3661 cgcctcctcg atacaggctg taatgacctc gacatggtga cgactggcta cttctggcac
     3721 tacgccccca aaacgtttgt gactctcaat ttgactagca atgacattgg acaagagctc
     3781 atcgtcgttt ttcaagacgg caacactggt ctcatcacag gatgtctcaa atgctaaaat
     3841 atatctatcc ttcatctatt tctctcttca tgataatggc gtcctcgact gggtcatggt
     3901 agtaggcttt tcgctcagcg atgaccgcca ttttttcttt cttgtaaaat gcttgcgctc
     3961 gttgatttga ctgtctaact tcgaggaaaa tttccttgtc tgtcggcaat tgagcaaaca
     4021 aggctgacgc aattccctga ccctgacagg ctcctttgac agcgatttgc aggacttctg
     4081 cttcaaagag agtctcctga acagttagaa agccaatcac ttctgcccca tcataggcca
     4141 gagcatacca agtctggtct tgggacagat ctgcttggat ttgttctagt gtccaaggac
     4201 tgactgggta aacagctgcc atgacagcgt agatatcttg agccaaggca ggttgctgtt
     4261 ggatacgttt gatttctatc ataggcgttt aatgtaagac tcaccagact cggtatggtt
     4321 cttgagccag ttttcctctg cctcaacacg tttgaggtaa tttggcacaa aatcatgcaa
     4381 ggagtctgct tccttgtccc aggccaaaag agctagatta gctgcattgg gcaatgtttc
     4441 tttgtaatca gtccttggca agtgtttttg aatctgctca acaaaggggc caacttctcc
     4501 gacaaaggtt acctgactag cgcccttgat tagctcaatg actcgctcaa aaggcagatg
     4561 tgcttctgcc atgacaggtt tggcattttc ataaaatcct gcataaacat tattgcgacg
     4621 cgcatccatc aaaggaacaa acaaaccttc ttgttgatat ggcactagag ccaagagact
     4681 agacatacca accaactcaa tgttcagggt gtaagctaag gtcttagcag ttgctaccgc
     4741 aattcgcaag cctgtatagc tacccggccc ttcagctacc acgattcggt ccaaatcctt
     4801 gggtgtccaa tccaaacttg ccatcaaaaa atcgatggca ggcataagag taatactgtg
     4861 atttttctta atattaatcg tcgtctcggc aagaacctgc ttatcctcta aaatagccag
     4921 agaaagagcc ttgctggacg tatcaaaagc taatactttc ataacacatt cctatctttt
     4981 tgtctgctta ctattatact acaaaagctg gcacatggga attttctttg cccccagaca
     5041 agagtgccct cacttaacta aaaataattt aaaaaaatgc tcacttttcc ttttcttttc
     5101 cgaatataaa agtgaacaag aaaaaaggag gaaagttcaa tgacaaattt tgacattctt
     5161 gacaatcaat ttttatcctt atctgaaaat gaattatcag atattgatgg cggtctcgct
     5221 cccttggtta tctttggagt agcagtatct tggaaggcta ttgcaggtgg aacagcactt
     5281 ataggttctg gtttggcagc tggttatttt ttaggaggag attaatatga tgaaagattt
     5341 gaacaattat cgtgaaattt ctaataagga attgcaagaa atcaagggtg gctttggtgt
     5401 cggtgttggt atcgctttat ttatggcagg ttataccatt ggaaaagacc ttcgtaaaaa
     5461 gtttggtaag tcatgctaga taagaaacac atttttagaa ggataaattt tattgtcttc
     5521 atctcttaca gtttgctcag cattctcaat gatttgaaca ttactaccat ccctttacca
     5581 ttcgatttat ctgtttgtat tgttttattt ttatgcttca actctatttt tgagtagaac
     5641 aatgactccc ataaaaataa taagctttga aaattccatt gtcatgtcat gttagaaaaa
     5701 tgcaaagacc acctcatctt gatagatggg gtggaatttt cgtgtcgtaa atctactatc
     5761 tctacattcc caaacaaaaa aaaccagcat aagcagggca tctaagcatt taatccaaag
     5821 taaaatacaa accaaacgac ataggtcacg aggaggagaa aaagcgagta gagagtcaca
     5881 aaggtaattt tccacaagaa cttggtttgt catcgttcca gtttggcaaa tagaagattt
     5941 cctccataaa cgcaagcaac aaaaacgata aaagctagca aacgaactcc gatagcaaaa
     6001 gcaaataagt tatacatagg gcaacctcct tgacttaaaa tctatatgga attatgacaa
     6061 gtaataaact tcacttccgt tatcaacata acacattttc tttatttttg caaacgatta
     6121 ccaaagaaat cgccccctaa ctttctcgtt tccgtctttt actaattttt cattttgtgg
     6181 tataattgaa ataattgtaa cgaatcaagg tcaatctaga cacaaaatgg aatgaaatca
     6241 agcaaatatc tgctaaaagt ttggaataag ctgacctgta aatagaaagg aactatatga
     6301 tttacaaagt tttttatcaa gaaacaaaag aacgtagccc acgccgtgaa acaacacgcg
     6361 cgctttacct agacatcgat gccagctcag aacttgaggg tcgcatcact gctcgccaac
     6421 ttgtcgaaga aaatcgccca gagtacaata tcgaatatat cgaactcttg tctgacaaat
     6481 tgctcgatta cgaaaaagaa actggcgcct tcgaaatcac ggagttctaa tatggcctac
     6541 actcttaaac ctgaagaagt cggcgttttt gccatcggtg gtctaggaga aatcgggaaa
     6601 aacacttacg gaattgaata ccaagacgag attatcatcg tcgatgctgg gattaaattc
     6661 ccagaagatg acttgcttgg tatcgactat gtcattcctg actactctta catcgtagac
     6721 aatatcgacc gcgtcaaggc tgttttaatc acacacggac acgaggacca cattggtggg
     6781 attccgttcc tactcaagca agcaaatgtc cctatttatg ctggaccgct tgccttggct
     6841 ttgatccgtg ggaaactcga agaacacggc ctcttgcgca acgccaaact ttacgaaatc
     6901 aaccacaaca ccgagttgac ctttaaaaat ctcaaggcaa ctttctttag aacgactcac
     6961 tctattccag agcctttggg gattgtcatt catactcctc aagggaaaat cgtctgtacg
     7021 ggtgacttta agttcgactt tactccagtt ggagaacctg cggacttgca tcgtatggct
     7081 gcgcttggtg aagaaggcgt gctctgtctc ctgtctgact cgacaaatgc ggaagtacca
     7141 acctttacca actctgaaaa agtcgttggt cagtccatta tgaagattat ccaaggtatt
     7201 gaaggacgta tcatctttgc atcctttgcc tcaaatatct tccgtctcca gcaggcaaca
     7261 gaagctgctg ttaagactgg acgcaagatt gcggtctttg gtcgttctat ggaaaaggcc
     7321 attgtcaacg gaatcgatct tggctacatc aaagctccta agggaacctt tatcgagcca
     7381 aatgaaatca aagattatcc tgcaggagaa gttcttatcc tctgtacagg tagtcagggt
     7441 gagcctatgg cagccctctc tcgtatcgcc aacggaaccc accgtcaagt acaattacaa
     7501 ccaggtgata ccgttatctt ctcttctagt cccatccctg gaaacactac tagcgtcaac
     7561 aagctgatta acatcatttc tgaagctggt gtcgaagtta tccacggtaa agtgaacaat
     7621 atccatacat ctggacacgg tggtcagcaa gagcaaaaac tcatgctctg cttgattaag
     7681 ccaaaatact tcatgcctgt ccacggtgaa taccgcatgc aaaaagtcca cgctggacta
     7741 gcagtggata ctggtgttga gaaggacaat atctttatca tgagcaatgg cgatgtgctt
     7801 gcccttactg ctgactcagc tcgtatcgca ggtcatttca acgcccaaga tatctatgtc
     7861 gatggaaatc gtatcggtga aattggcgca gctgtcctca aagatcgtcg cgatctatct
     7921 gaagacggtg tcgttctggc agtcgcaact gttgacttca aatcgcagat gattctatct
     7981 ggtccagaca tcctcagccg aggctttgtc tacatgagag agtctggcga cttgattcgc
     8041 caaagccagc gtatcctctt caatgccatt cgtatcgcac tgaaaaataa ggatgctagc
     8101 gtgcaatctg tcaatggtgc cattgtcaac gctattcgcc ccttcctcta tgaaaatacc
     8161 gaacgtgaac cgatcatcat cccgatgatc ctcacaccag atgaagaata aagcaagaaa
     8221 acagccccgt cttcggagct gtttttctct atgctttctt ttgagattaa aactcaatga
     8281 aaatcagacc agaaggctag gaaaatgcat aacttgagtt agctcccata gttcggggaa
     8341 ctatgggagg ttggagatga atcaaagcca agctttgaac tcattcgtaa gaagccgacg
     8401 acgtatcatt ttgatttttg aagagtttta gaaatactac gatttttacc ttccagatac
     8461 accatcaaaa tagaaatatc tgctgggttt actcccgaaa tacggctggc ttggccgatg
     8521 gtttctggat tgatgagttt gaacttctga cgagcttccg tcgcaataga atcgatgtca
     8581 tcccagtcga tattggctgg aatgcgtttt tcttccatac gtttcatctt ggcaacctga
     8641 tccatggctt tggaaatata gccttcgtac ttgatttctg tttcaatcaa ttcgataatc
     8701 ttgtcatcca agtcttctgc agctggtccg atgaaggcca ccacatcttg gtaagaaact
     8761 tctggacggc gaaggaattc cttggctgtc accgcatctg tcaacggctt gaagcccatt
     8821 tcctcaacct tagcattggt ttccttgact ggcttgagtt tgatactgtc taggcgtttc
     8881 atctcattat caaattgatt tttcttgatt tcaaaactag cccagcgttc atcatccaca
     8941 aggccaatct cgcgtcccat ctcagtcaag cgcatatcag cattgtcatg acgaagaatg
     9001 agacggtatt cagcacgact tgtcaagaga cggtagggtt caatggttcc cttggtcacc
     9061 aagtcgtcga tcatcacccc gatataaccg tcactgcgct tcaaaatcaa ctcaggcttg
     9121 ccttggattt tcagagccgc attgataccc gcgataatcc cttggccagc agcttcttca
     9181 taacctgatg ttccatttgt ctgaccagca gtgaagagac ctgagatttt cttggtttcc
     9241 aaagtcgcac gcaactgatg aggcaagacc atatcatact caatagcata acctgtccgc
     9301 atcatctctg cattttccaa acctttgatg gaatgaacca agtcacgctg gacatcctca
     9361 ggcagactgg ttgaaagtcc ttgaacatag acttcctcag tattgcgccc ttctggctca
     9421 aggaagagtt ggtgacgttc cttgtccgca aagcgcacaa tcttgtcttc aatcgacgga
     9481 cagtaacgag gccccactcc cttgaccaca cctgtaaaca taggcgcacg gtggaggttg
     9541 ttttggataa tctcatgact ggtaccattg gtgtaggtca accagcatgg tacttggtcc
     9601 ttgacataat cctcatcacg tgaagtgtat gagaagtgat taggtgcttc atctcctggt
     9661 tggatttctg tcacgtcgta gttgatagaa gaggccttga cacgtggagg ggttcctgtc
     9721 ttgaaacgac cgatttcgag acccagttcc ttgagattgt cagctaggtt aatagaagcc
     9781 aagctgtggt taggacctga tgagtacttg aggtctccga tgataatttc cccacggaga
     9841 gcagtccctg tcgtcacaat aacagcctta gcagcatatt cttgatgggt ggctgtacgc
     9901 acaccgacaa ccttaccatc ttccaccaaa atctcatcaa tcatggtttg acgaagggtc
     9961 agattttctt gattttcaac tgtcttgcgc atttccttag agtaaagttc cttgtcagcc
    10021 tgcgcacgaa gagcacgaac agctggcccc ttccctgtgt tgagcatctt catctggatg
    10081 taagtcttgt caatagtctt ggccatctcg ccaccgaggg catcgacttc acgcacgaca
    10141 atccccttgg cagaaccacc gatagaggga ttacaaggca tgaaagccag catttcaata
    10201 ttgatggtcg caagcaggac cttacagccc atacggctag cggccaagga agcctcaacc
    10261 ccagcgtgtc ccgcaccaat tacaataata tcgtattctt cagtaaaatg ataagtcatg
    10321 tttctctcct attcctcaag atgaatgtgt cttagttggc cttcccaatc tggtagggct
    10381 gtttttaaaa aggctggaac tagctggata tcctcaagct tatccaagtc aatccactca
    10441 cagggctgcc ttttctcatc ttcctgcatg gtcaacgggg catcttcaag caaatccacc
    10501 agataatgaa actcgatatt gtgataggaa acgccgtcca cttcaaaacg attttcaacc
    10561 acaaaagcta gctgcccagc ttgagcttta acacccagtt cttccttcac ttcacggact
    10621 accgcgtctt ccgttttttc gttaacttga atcgcaccgc cgataatgta atacttgccc
    10681 ttgtctttgg tgactagaag cttgtgattt tggacaatca aggctgtcgc ccgaacacca
    10741 aaaaccgtat tgtctacttt tgtccgaaag tcttgttgag tcattcttgt cctttccctt
    10801 aaacgacaca aaaacagtca aaactccaaa gaagtgcagg acaaaaaagc ctgcaacatc
    10861 catgagcttt gaccatcatt tctattgctg ttattattgt agcaaattga gaaaatttgt
    10921 caatctaaat gtactgacaa acttgtccat cgcggtaagc attgtcaatc aaaccgccac
    10981 ctagacactc ttcgccatcg taaaagacaa ctgcctgtcc tggtgtaatc gcgcgttgtg
    11041 gttccgcaaa gataacttct gccttgtctc ctttgacatg gacggtcacc ttagagtcag
    11101 gctgacggta acggaattta gccgtacatt ctagcgtaaa ctcttctggc atttcacgag
    11161 taaagtggac ttggctagcc tctaggctgg ttgacatgag cgaatcatgg tagaaacctt
    11221 ggcctacata gagaatattc ttgcttagat cttttccgac aacgaaccaa ggggcattgt
    11281 caccaccgtg ttgcccaccg ataccgagtc cgccacgctg accgattgta tagtacataa
    11341 gaccagcatg ctcgcccata tcacgaccat ccacagtcat catacgacca ggctgagctg
    11401 gcaggtagtt gctgagaaag tttttaaagt tcttttctcc gataaagcaa atccctgtcg
    11461 agtctttctt cttagcagtc gaaagacctg cttcttctgc tagttttcga acttcaggct
    11521 ttttcaaatg tcctagtggg aacatggttt tttgaagttg ttcttgcgaa agttggctga
    11581 ggaaataggt ctggtccttg ccattgtcca cgccacgaag catgtgaaca gtgccatcct
    11641 catcacgcgc cactcgagca taatgcccag tcgctacata gtctgccccc aaggttatgg
    11701 catagtccaa aaaggccttg aacttgattt ccttgttgca cataacgtcc ggatttggcg
    11761 tgcgccctgc acggtattcc gctaggaaat actcaaaaac gcggtcccag tactcttttt
    11821 caaaattgac agagtagtag ggaatgccaa tctggtctgc caccgcaacc acatccttgt
    11881 aatcttcggt cgccgtacag acgccgtttt catctgtgtc atcccagttc ttcatgaaga
    11941 taccgatcac atcgtagccc tgctccttga gcaaaagagc cgtcaccgac gaatcaacac
    12001 caccactcat ccccacgaca acacgtgttt tagagttatc actcatggta agtctcccat
    12061 ctattcgttt attcacgatt gaaggtcgtg tgtgcttcac gattgaaggt cgcttgagca
    12121 gtattcatta taacacgctt ggttagagaa gacaaggaag agcttaaaaa ttgtttttta
    12181 aaacttatag tcaatataca gattatgtga atgatgtgag tataccttct tcttatcttc
    12241 attcaaataa ctataataaa taaaacaacc cattttctct aatctatttt tcaagttaga
    12301 gaaaaggagt cgttttttta atgacatttc ctgaaatcat aagataggta cctaaagaat
    12361 acgaacataa atctattcaa tcgaacctta tctttgtttc aaaattaaaa tgtcaaatgt
    12421 tcttaacatg atttaattta atcggcttaa acccattcac cgttttcatt gacagtatat
    12481 ccgtctacag ttgtgttgac tgcaagggca cctaagccat tgacatagta ccatttatct
    12541 gatactttga accattggtt tgctttcata gcacctgatg cttcaagata gtaccaggta
    12601 tctccatctt tcacccaacc tgttgccatt gaaccgttag cgttgaggta gtaccatgaa
    12661 ccgttgactt tagcccaacc tgttgccata gcgccgttag cgtttagata gtaccatgaa
    12721 ccattgtatt ggagccaacc tgtcgccata gcgccattgc tgttgaggta gtaccatgag
    12781 ccattgtatt ctagccaacc tgtcgccata gcaccattag cgttgaggta gtaccatgaa
    12841 ccattgtatt ctagccaacc tgtcgccata gcaccattag tattgaggta gtaccatgaa
    12901 tcattgtatt ggagccatcc tgtcgccata gcgccattgc tgttgaggta ataccataag
    12961 ccgttgactt tagcccaacc tgttgccata gcaccattag cgttgaggta gtaccatgag
    13021 ccattatttt gtagccagcc tgttgccatt gaaccatccg tattgtagaa gtaccacata
    13081 ccattttctt gtttccagcc tgtttttgga gtttctggtt taggagctgg agctggttta
    13141 ggagccgg
//
LOCUS       DAVLRT010000050        13027 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLRT010000050 DAVLRT010000000
VERSION     DAVLRT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.050"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            118..1599
                     /locus_tag="VXM85_001450"
     CDS             118..1599
                     /locus_tag="VXM85_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1346007.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1961..2962
                     /locus_tag="VXM85_001451"
     CDS             1961..2962
                     /locus_tag="VXM85_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1346008.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3113..4030
                     /locus_tag="VXM85_001452"
     CDS             3113..4030
                     /locus_tag="VXM85_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1346009.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4041..4931
                     /locus_tag="VXM85_001453"
     CDS             4041..4931
                     /locus_tag="VXM85_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1346010.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4960..6576
                     /locus_tag="VXM85_001454"
     CDS             4960..6576
                     /locus_tag="VXM85_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1346011.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVENLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6586..7905
                     /locus_tag="VXM85_001455"
     CDS             6586..7905
                     /locus_tag="VXM85_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1346012.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8115..8187)
                     /locus_tag="VXM85_001456"
     tRNA            complement(8115..8187)
                     /locus_tag="VXM85_001456"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8152..8154),aa:Lys,seq:ctt)
     gene            complement(8452..9723)
                     /locus_tag="VXM85_001457"
     CDS             complement(8452..9723)
                     /locus_tag="VXM85_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1346013.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            9993..10193
                     /locus_tag="VXM85_001458"
     CDS             9993..10193
                     /locus_tag="VXM85_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346014.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            10397..10576
                     /locus_tag="VXM85_001459"
     CDS             10397..10576
                     /locus_tag="VXM85_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346015.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10713..10892
                     /locus_tag="VXM85_001460"
     CDS             10713..10892
                     /locus_tag="VXM85_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1346016.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            10929..11381
                     /locus_tag="VXM85_001461"
     CDS             10929..11381
                     /locus_tag="VXM85_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1346017.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11672..12142
                     /locus_tag="VXM85_001462"
     CDS             11672..12142
                     /locus_tag="VXM85_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1346018.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFIMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12198..12740
                     /locus_tag="VXM85_001463"
     CDS             12198..12740
                     /locus_tag="VXM85_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346019.1"
                     /translation="MLEKVKQFFRSRSAKTEPSVDILPRNRFEDLDFERVLKSGARRL
                     VNEEGRYAEDGKITELEFPEDFAEFEFLVGFKTEEEEQFQQLLARLNSIDNAIQSYLE
                     SEMQQPIPQYAKDLGYTQKRWEKTFYFHPWILSGEENPPNLRYVADYVNDEFTVYFAK
                     KHGRWQAYWDAECQKVIEES"
     gene            12790..12954
                     /locus_tag="VXM85_001464"
     CDS             12790..12954
                     /locus_tag="VXM85_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEV1346020.1"
                     /translation="MELLNSRMDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPG
                     GGKSVRENLI"
BASE COUNT         4219 a         2115 c         2686 g         4007 t
ORIGIN      
        1 tgttattata cgaaataaaa gattttagga aaagtcgtta tatcatgcta tacctattct
       61 ttgtggtata attgcaagag gtttaatccg ataatttata aagagagaaa gatattcatg
      121 agagatttat tatctaaaaa aagtcatagg caattagaat tattagaatt attatttgaa
      181 cataaacgtt ggtttcatcg ttctgaacta gcagagttat taaattgtac agaacgtgca
      241 gtcaaagatg atctatccca tgttaaatct gcttttcctg acttgatttt tcattcttct
      301 actaatggta tacgcattat taataccgat gatagtgata ttgaaatggt ttaccatcat
      361 ttctttaaac attcaactca tttttcgatt ttagaattca tcttctttaa tgaaggttgt
      421 caagctgaga gtatttgtaa agaattttat atcagttcat cttcgctcta tcgtattatt
      481 agccaaatca ataaagtgat taaaaggcaa tttcaatttg aagtcagtct gacccctgtt
      541 caaatcattg gaaatgagag agacattcgt tacttttttg cacaatattt ttcagaaaaa
      601 tattatttcc tagaatggcc atttgaaaat ttttcatcag agccactatc tcaattgtta
      661 gaattggttt ataaggaaac aagctttcca atgaatttgt caacgcatag aatgctaaag
      721 ttgctcctag ttacgaacct atatagaata aagtttggtc attttatgga agtagataaa
      781 gattctttta acgaccaaag tttggatttt ttaatgcagg cagaaggaat agaaggtgtt
      841 gctcagagtt ttgaatcaga atacaatatc tctttagatg aagaagttgt ttgccaatta
      901 tttgtgtctt actttcaaaa aatgtttttc atagatgaaa gtctctttat gaaatgcgta
      961 aaaaaggata gttatgttga aaaatcttac catctattga gtgattttat tgatcagatt
     1021 tcagtcaagt atcagattga aatggagaat aaggataatc tgatttggca tctgcataat
     1081 accgcacatc tgtatcgtca agagttgttt actgagttta ttttatttga tcaaaaaggg
     1141 aatacaatca ggaactttca aaatattttt ccaaaatttg tttcagatat aaaaaaagag
     1201 ctttctcact atttagagac tcttgaggtt tgttctagtt caatgatggt aaatcatcta
     1261 tcttatacct tcatcactca taccaaacat ttggtgatta atttgctaca aaatcagcct
     1321 aaactaaagg ttttagttat gagtaatttt gatcagtatc atgcaaaatt cgttgcagag
     1381 acactttctt attactgtag caataatttt gaactggaag tttggactga attagaatta
     1441 tcaaaggaat ctttagaaga ttcaccttat gatatcatta tttctaattt tattattcct
     1501 cctattgaaa ataagagact catctattca aataatataa acacggtctc actcatatac
     1561 ttgttaaatg ccatgatgtt tattcgatta gatgagtaac caaaaataac aaaaaaacgc
     1621 tatttcgcgt ttgaactgta cctcaaaagt tagacagaaa aaatctaatt tttggggtgt
     1681 ttttgttaag aaaagacctt caatcattta agcaaaatgt acatctagat ttatagccct
     1741 ctaaaactct tgcttttttt gtagaagata cgaaaaaagc cataaaacca aggcttttcc
     1801 tgttttatgt agatgtttac acatgaaaat tgaagtattt tcaaaagcta attggagtga
     1861 ttttattcat gatgggataa atgatctggc caaaaatgat tgagaaatag ttgcttaaaa
     1921 agcatttgca tgatactata tattgaaagg aggtgataat atggtagaac aaagaaaatc
     1981 aattaccatg aaagatgttg ctttagaagc aggagttagt gttggaactg tttcacgtgt
     2041 aattaataaa gaaaaaggca ttaaagaagt aactttgaaa aaagtggaac aagcgattaa
     2101 aactttgaat tacattccag attactatgc tagaggaatg aaaaaaaatc gaacagaaac
     2161 gattgcaatc attgtaccaa gtatctggca tcccttcttt tcagaatttg ctatgcatgt
     2221 ggaaaatgaa gtctataaga gaaataacaa attactctta tgttctataa atggtacaaa
     2281 tagagagcaa gactatctgg agatgttgcg tcataataaa gttgatggag tggttgccat
     2341 tacctatagt ccaattgaac attacttgac gtcaggaatt ccctttgtta gtattgaccg
     2401 cacatactca gatattgcca ttccttgtgt ttcatcagat aatgatgcag gtggaagaga
     2461 agcagctaaa caattaatta gtaaaggatg tcagcattta gcctttgtgg gcggacataa
     2521 tacaaccatt aatgaaacca aacgtcgtag aatttcattt gaaaagtatg tccaagagca
     2581 taaaatacct cttagtattt ttgatttgga tgagacagtt actgactatt atggaaagct
     2641 agagagtttc ttggaagaaa attcttctat agatggaatt tttacaatca acgattttac
     2701 agctttggat gtgattgaaa tactagaaaa aaatggcaaa cgtattcctc atgatgtcca
     2761 aattattgga tacgatggaa ttaaaatggc tggagataga gattatctac tttcaactat
     2821 tgaacaacca ttggaagaaa tggcaaaaga agcggttcgt attttgtttg atataattga
     2881 tggaaagact gttaatttgc agacaatcct accagtaaaa tttgttgaag gaaaaacaac
     2941 aaaaaatgaa aataagtctt gacagaaagc gctatcaatg atagaatgaa ttcagataaa
     3001 aagatttatt tttaaaacaa aaatgaaacg tttcaaaaaa agaaataaaa agacagcgcc
     3061 aagcgctatc ttttatagaa aaaatgaaac gtttcaaaaa aaggaggttg ctatgaatag
     3121 caaagcgaag caagtttctc tttgggaaag aatcaagaaa caaaaactct tgttattgat
     3181 gactgtcccc ggtttagttt taacctttat ctttaaatac atccctatgt atggggtttt
     3241 aatcgcattt aaagattaca atcctttaaa aggaatttta gggagtgatt ggattggttt
     3301 ttctgagttt acaaaattca tatcctctcc caactttggt atcttgttag ccaacacatt
     3361 aaaattaagt atctatggtt tattgcttgg ctttttacca ccaatcattc ttgcgattat
     3421 gctcaatcaa ctcttgagtg aaaaagtcaa aaaacgaatt cagctcattt tatacgcacc
     3481 aaactttatc tcagtcgttg ttattgtcgg tatgattttc ctcttctttt cagtgggagg
     3541 accaatcaac aattttcttt ctatgtttgg aatgaaggct gacttcttga caaatccaga
     3601 cttctttaga cccttataca tctttagtgg tatctggcaa ggaatgggct gggcttcaac
     3661 gctctacacg gcaacattgg taaatgtaga tccagcctta gtagaagcag ctcgactgga
     3721 tggagccaat atcttccaac gaatctggca cattgatatt ccagctctta agcctattat
     3781 ggttatccaa tttgttttag ctgcaggtgg aattatgaat gtcggatatg aaaaagcatt
     3841 cttgatgcag acatcgttaa atttgccaac ttctgaaatt atctcgacat atgtctataa
     3901 agttggtctt gtatcaggag actattctta ctcaacagcg gttggtttgt ttaatgcagt
     3961 gattaacgta gtattgcttg ttgcagttaa ccaaatcgtt aaacgcatga ataatggtga
     4021 aggaatttaa ggaggaaagt atgaaaaatt cgattatgga tacaaaattt gatagacgta
     4081 tcttactctt aaataaaatc attattgtct ttatcgtttt gatgactttg cttcctttac
     4141 tttatatcgt cgtagcatcc tttatggatc ctaaggttct ggttagtaga gggattagct
     4201 ttaatccagc cgattggact gtagaaggtt accagcgtgt attcagtgac caatctattc
     4261 taagaggttt tatcaattct ctactatact cttttggatt tgcagcttta acagtattgc
     4321 tatctgtgtt tacagcttat cctctttcta agaaagactt ggttggacgt cgttggatta
     4381 actacttctt gattgtgact atgttctttg gtggtggttt agtcccaact tacttgctcg
     4441 taaaagaatt gggaatgctc aatactccat gggctatcat tgttccaggt gctgtcaatg
     4501 tttggaatat tattcttgct agggcctatt tccaaggatt gcctgaagaa ttagttgaag
     4561 ctgctgtcat tgatggagcg aatgacttgc agattttctt taaaatcatg cttcctcttg
     4621 caaaaccaat tatgtttgtt ctcttcctct atgcttttgt aggacagtgg aactcatact
     4681 ttgatgcaat gatttatatc aaggatccaa acttggaacc attgcaactt gtacttcgta
     4741 aaattctcat tcagagccaa ccaggtcaag acatgattgg agcacaagcg gctatgaatg
     4801 aaatgaaacg tttagctgaa ttgattaaat acgcaactat tgtcatttcc agcttgccat
     4861 tgattgttat gtatccattc ttccaaaaat actttgataa aggaattatg gctggttcac
     4921 ttaaaggata aaaaaagaaa aaataaaagg agttttctca tgaaattcaa aacattctca
     4981 aaatcagcag ttttgttgac agctagttta gcagtacttg cagcctgtgg ctcaaaaaat
     5041 acagcttcaa gtccagatta taagttggaa ggtgtaacat tcccgcttca agaaaagaaa
     5101 acattgaagt ttatgacagc cagttcaccg ttatctccta aagacccaaa tgaaaagtta
     5161 attttgcaac gtttggagaa ggaaactggc gttcatattg actggaccaa ctaccaatcc
     5221 gactttgcag aaaaacgtaa cttggatatt tctagtggtg atttaccaga tgctatccac
     5281 aacgacggag cttcagatgt ggacttgatg aactgggcta aaaaaggtgt tattattcca
     5341 gttgaaaatt tgattgataa atacatgcca aatcttaaga aaattttgga tgagaaacca
     5401 gagtacaagg ccttgatgac agcacctgat gggcacattt actcattccc atggattgaa
     5461 gagcttggag atggtaaaga gtctattcac agcgtcaacg atatggcttg gattaacaaa
     5521 gattggctta agaaacttgg tcttgaaatg ccaaaaacta ctgatgattt gattaaagtc
     5581 ctagaagctt tcaaaaacgg ggatccaaat ggaaatggag aggctgatga aattccattt
     5641 tcatttatta gtggtaacgg aaacgaagat tttaaattcc tatttgctgc atttggtata
     5701 ggggataacg atgatcattt agtagtagga aatgatggca aagttgattt cacagcagat
     5761 aacgataact ataaagaagg tgtcaaattt atccgtcaat tgcaagaaaa aggcttgatt
     5821 gataaagaag ctttcgaaca tgattggaat agttacattg ctaaaggtca tgatcagaaa
     5881 tttggtgttt actttacatg ggataagaat aatgttactg gaagtaacga aagttatgat
     5941 gttttaccag tacttgctgg accaagtggt caaaaacacg tagctcgtac aaacggtatg
     6001 ggatttgcac gtgacaagat ggttattacc agtgtaaaca aaaacctaga attgacagct
     6061 aaatggattg atgcacaata cgctccactc caatctgtgc aaaataactg gggaacttac
     6121 ggagatgaca aacaacaaaa catttttgaa ttggatcaag cgtcaaatag tctaaaacac
     6181 ttaccactaa acggaactgc accagcagaa cttcgtcaaa agactgaagt aggaggacca
     6241 ctagctattc tagattcata ctatggtaaa gtaacaacca tgcctgatga tgccaaatgg
     6301 cgtttggatc ttatcaaaga atattatgtt ccttacatga gcaatgacaa taactatcca
     6361 agagtcttta tgacacagga agatttggac aagattgccc atatcgaagc agatatgaat
     6421 gactatatct accgtaaacg tgctgaatgg attgtaaatg gcaatattga tactgagtgg
     6481 gatgattaca agaaagaact tgaaaaatac ggactttctg attacctcgc tattaaacaa
     6541 aaatactacg atcaatacca agcaaacaaa aactagaggt tgattatggg agataagaaa
     6601 tacacagtag aaaaagccaa tcgttttata gcagaaaata aacatctcgt taatactcaa
     6661 tataagcctg aagaacattt ttcagctgag attggttgga tcaatgatcc aaatggattt
     6721 gtctattttc gtggagaata ccatctcttt tatcaattct atccatatga tagtgtttgg
     6781 gggcctatgc actggggaca tgctaaaagt aaggacttgg tgacttggga gcacttgcca
     6841 gtggcacttg ctcctgacca agattatgac cgaaatggtt gtttctcagg ctctgccatt
     6901 gtcaaggatg atcgcctctg gctcatgtac actggacata tcgaagaaga aaccggtgtc
     6961 cgccaagtgc aaaatatggc attttcagat gacgggattc actttgaaaa gattgaacaa
     7021 aatccagttg caactggatc agacttgcca gatgagttga ttgctgctga ttttcgtgat
     7081 ccaaaactct ttgaaaaaga tggacgctat tactccgtag tagctgccaa acacaagaat
     7141 aatgtgggct gtatcgttct actagggtcc gataacctag tagaatggca gttcgaatcc
     7201 atctttttaa aaggggtaga acaccaaggc tttatgtggg aatgcccaga ttacttcgag
     7261 ttagatggga aagattgcct tattatgtca cccatgcgtt atcagcgtga gggagactca
     7321 tatcataaca tcaactcatc gcttttgttc acgggtaagg tagattgggg agaaaaatgt
     7381 tttatcccag aatcagttca agaaattgat catggccaag acttctatgc gcctcaaaca
     7441 ttgttggacg atcaaaatcg tcgtatcctg attgcttgga tgcagacatg ggggcgtacc
     7501 cttccaaccc atgaccaaga acacaaatgg gcatgtgcca tgactttacc tagaatttta
     7561 agattggaag atggcaaact aagacaattc cctgttaaaa aaggccaata tcaaatccaa
     7621 atagataaag attgtcatta ccacttagga aatgatatag attatcttga atttggttat
     7681 gatagtaatg cgcagcaagt ttacattgat cgtagccatc ttattcaaaa aattctaggt
     7741 gaagaagaac aggacactag tcgacggtat gtagatattg aagctaaaga attggaagtt
     7801 gttctagata aaaattccat cgagattttt gtcaatcaag gtgaagcaag cttgactgca
     7861 acttattact taacggtgcc agctgagcta tcacgaattg attaaaaatt aagttatttc
     7921 tcctaaagaa aaagttctct ttctaaaata gtggaaagag gactttttgt gttttgggta
     7981 tataagctta gtttatggta tttgtaaaat tggtgttgga ttatgattta agctagtttt
     8041 ctaaagaatt tgaaaaaaat tttatttaag caaaaaaaac ccttggttcc aaggcttttc
     8101 ctgttgtatt tatatgcccc ctacagggat cgaacctgtg acccacggat taagagtccg
     8161 ctgctctgcc agctgagcta aggaggcaaa gaaaaaagct gtattggtgc cgaaacttca
     8221 cggtttgtat tgaacccgcg caattaagca ggtgggcaac tcgctctaac tgaagctgtt
     8281 tccgtgtgag acggcctaca tgctgttaga agacttttgt ttccctaata atacaaaaaa
     8341 tagtcggtca acacttaagt gtgaagtcgt acaccacagc gtttctatac ttatatgata
     8401 ccactttttc aaaaaaaatc aagaggaaag tgcaattttt tgaaaaggat ttcagatttt
     8461 tcgcaaacgg tcgatagctt cctttctttg agccaaagcc cgttcatatt taccagtatc
     8521 ttctgcttgg aaatagtgat gattacgaat tttttctggc agatagtctt gcttgaccca
     8581 atttccagga tagttgtgtg gatagagata gtcttgggca ttccccagtt ccttgcttcc
     8641 actgtagtgc ccatcacgta ggtgtcgcgg aataggcaag tgccctgatg ttttgaggtc
     8701 agcaagtgcc ttatccatag ctacataggc tgagttggat tttggagaaa gggccaaatc
     8761 aatcacgaca ttggcaatga gaatgcgggc ttctgggaac ccaatcttct gggcagcatc
     8821 cagagcagtc acggtatgaa tctgggcctc aggattggct aagccgatat cttcataggc
     8881 gataacagtc aagcgacgag cgagactagg cagatcccca gcctcaatca agcgggcagt
     8941 atagtggaga ctggcatcca catctgagcc acgaatagac ttttgcaggg cagatagaac
     9001 atcatagtga ccgtctccat ccttgtccat agtgatgtag cttctctgaa gactattttc
     9061 catgatgtct aaggtgatgt ggcgaatgcc ctcgtcattc tcaggggtag agagaacagc
     9121 taagtccagt gagttaaagg cagagcgaag gtctccgttt gtagaggttg cgatgaaatc
     9181 cagcgcatcc tcatctagtt ctattggaaa atcaaaacca cgttcagggt tacttagagc
     9241 tatctgcagg gcctctttga cgtcttggtt agacagaggt tccaactcga aaatttgaac
     9301 tcggctacga atggcaggag tgacagagaa gaaaggattt tcagtcgttg ctccaatcat
     9361 gatgaccagt ccactttcca agagaggcaa gaggaagtct tgcttggtct tatctagtcg
     9421 atgaatttcg tctagcaata ggacgagacc accagaaaat ttagcttctt ccgagatttc
     9481 ttgcagtcgc tttttactat caactgtcgc attgaaagtt cgaaaggcat acttggtcgt
     9541 tccagcgatg gcagaggcaa tactggtttt gccgattcca ggagggccat atagaatcat
     9601 ggaggacagg cggttggctt ccaccatgcg gcggatgatt tttccaggtc cgaccagatg
     9661 ctcctgaccg atgacctggt cgatggtttt agggcgcatg cgaagcgcga gattgtctgg
     9721 catagcagtc ctttctagcg tggatttgag tagaatgact catctactat atcttactat
     9781 gtccattcta gcctattagt tgtgatattt caatggtttt gtaataatca gggagtgaga
     9841 tagaatcttt tttactattt aatttaatga ggagaggtat cagcatgaga gatttgattt
     9901 tccttaaagc tcaaaatagg atagttgagc ctaaagaagt ggaagtgctg acttgagaaa
     9961 taatattgct gaatgtcatt gtatgaacta taatgaaaag agaggtgata agcaatggaa
    10021 atgatggacc aagtcaggaa attttgattt ttacaaaaca aattcgtcat tggattctta
    10081 gtgatcaagt tatttcaggg aaaagaaaac ttttcttccg ggaagatact ccaaaagaga
    10141 ttttagattt gtacgaaaac attaaatcta aacttgattt tgcttaccaa taagtttatt
    10201 ctaataatgg gttgaaaaaa tatgaaaaat agaataattg atgtttttga agtagtaaat
    10261 cgcatccttg taataactgt tgaaaatcct gatttcgaag acttgagagt taatcatact
    10321 caatgaaaat caaagagcaa actaggaaac tagccgcagg ctgtacttga gtacggtaag
    10381 gcgacgttga cgtggtttga atttgatttt cgaagagtat cagtttgtaa aaattggaga
    10441 taaaaaatat agagtacgca gtgttcctat gattcattca actccgcctc aatctgtctt
    10501 agatcgagat acttttacaa ttgactatac agatgatgaa ttgctcgata aagaagcagt
    10561 ttttactact cattagaaaa ataggtctga ataaattggc ttcccttaga accacatcta
    10621 ggggctttta tgtataatag aataataagt atcactcctt tacaatacgt gtaacacgtg
    10681 ttataatggt gttgtcagga ggtaaaagcg ctatgcctat gacacaaaaa gagatggtta
    10741 aactccttac ggcccatggt tggataaaga ctagaggcgg taaaggctcc catattaaaa
    10801 tggagaagca aggggaaaga cttatcacaa tccctcatgg tgagctgaat aagtacactg
    10861 aaagagggat aagaaagcaa gctgggttgt aaaattcagc ccctgttttt tgatatggag
    10921 gtagtgaaat gttagttacg tatccagctt tattttatta tgacgacaca gacggaacag
    10981 aagcgactta ttttgtccat ttcccagatt ttgaatactc agctacacaa ggagagggga
    11041 tttctgaggc tttggctatg gggtcggagt ggctagggat aactgttgca gatttgatag
    11101 agagtgatgg agagttacct cagccatcag atatcaatag cttgtcttta attgataatg
    11161 atccttttaa agatgatgaa gatttcgtgt caacctatga ccttgataaa tcttttattt
    11221 ctatggtatc tgttgatgta tcagaatacc taggaagtca ggaacccata aaaaagactt
    11281 tgaccatacc aaaatgggca gataagttgg gacgagaaat gggacttaac ttttctcaga
    11341 ctttgacaga cgctattgca gataagaaag ttcaagccta agattaagcg tggtatcgtg
    11401 gtaatagtca ataaaaaagc acgtccactt gtgctagtta ctcgcctatt gattttatag
    11461 atttattagc cctttcgagg gctttttata ttgattttta tgagaaatga agaaatcaga
    11521 cttttaagaa aatcactgat accaagggtt taaatgaggt aatatggtat aattaggaca
    11581 taaaataatt ttgtggtaag atggtagtat ctattttagc atatttccga gcaatcgggg
    11641 cgattaaaga gtcgcataga aagaggacaa aatggcaaca tacggatttt tagatgtttt
    11701 agaggaagag ttggacaaga actttccctt tgactttgag attagctggg acaaacgcaa
    11761 ccatgcggta gaagtgagtt ttctattgga agcgcaaaat gctgcaggtg tggagatggt
    11821 ggatgaagac ggagaggttt catcagatga cattcttttt gaggaagcgg tccttttcta
    11881 caatcctgcc aagtcaacag tcaatgagga agactatttg actgttattc cttacctgcc
    11941 taaaaaagga ttttcccgtg aatttttagc ttattttgcg cttttcctta aagatactgc
    12001 tgaggttggg ctagatgtcc tcatggactt tttggaagac ccagaagcag aggaatttat
    12061 catggaatgg aaccaagaag tctttgaaga aggaaaagtt ggcttggaag agggagaatt
    12121 ttatccttat ccaaggtatt agaaaagatt ctaagtggac atttttaaat aatgaattca
    12181 aggagaaggg ggagattatg ctagaaaaag tcaaacagtt tttcaggagc aggtcagcta
    12241 aaactgagcc gtcagttgat attcttcccc gcaatcgctt tgaggatcta gatttcgaga
    12301 gagtattgaa gtcaggagct cgtcgccttg ttaatgagga aggacgctat gctgaggatg
    12361 ggaaaatcac agaacttgaa tttcctgaag attttgcgga atttgaattt ctagttggtt
    12421 ttaagacgga ggaagaagag cagtttcagc aactattagc tcgtttaaat agtattgaca
    12481 atgctattca gtcctatttg gaaagtgaga tgcaacaacc catccctcag tatgccaagg
    12541 atctcggcta tactcagaag aggtgggaga aaacctttta cttccatcct tggatattaa
    12601 gtggtgagga aaacccgcct aatcttcgct atgttgcaga ttatgtaaat gatgagttta
    12661 cggtttattt tgctaaaaaa catggcagat ggcaagcata ctgggatgca gagtgccaaa
    12721 aagtaatcga agaaagctag ctcgtatttt cctagagagt aattgttatc atagaaaaga
    12781 gtaaatatca tggaattatt gaatagcaga atggatttcc aaggttgcaa gattgccctc
    12841 ttttgtgggg ataagctact gacgattttg cgtgatgaca aggacgatat cccttgtccc
    12901 aacatgtggg agttgccagg tgggggaaag agtgtcaggg aaaatctgat atagttttgc
    12961 attaaaagaa tatcctttca aggttattct tttttaattt tgtcccaaaa taattgactt
    13021 tgtccca
//
LOCUS       DAVLRT010000051        12896 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLRT010000051 DAVLRT010000000
VERSION     DAVLRT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.051"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..1079
                     /gene="dnaN"
                     /locus_tag="VXM85_001465"
     CDS             <1..1079
                     /gene="dnaN"
                     /locus_tag="VXM85_001465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1346021.1"
                     /translation="AISSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNED
                     AGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQ
                     YPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVAT
                     DSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENI
                     SFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKD
                     GVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAV
                     RPFTLVPADTDEDFMQLITPVRTN"
     gene            1144..1338
                     /locus_tag="VXM85_001466"
     CDS             1144..1338
                     /locus_tag="VXM85_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1346022.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1422..2537
                     /gene="ychF"
                     /locus_tag="VXM85_001467"
     CDS             1422..2537
                     /gene="ychF"
                     /locus_tag="VXM85_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1346023.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLIESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYESEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2608..3177
                     /locus_tag="VXM85_001468"
     CDS             2608..3177
                     /locus_tag="VXM85_001468"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1346024.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     KADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3178..6687
                     /gene="mfd"
                     /locus_tag="VXM85_001469"
     CDS             3178..6687
                     /gene="mfd"
                     /locus_tag="VXM85_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1346025.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            6755..7021
                     /locus_tag="VXM85_001470"
     CDS             6755..7021
                     /locus_tag="VXM85_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1346026.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7014..7382
                     /locus_tag="VXM85_001471"
     CDS             7014..7382
                     /locus_tag="VXM85_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1346027.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7502..8770
                     /locus_tag="VXM85_001472"
     CDS             7502..8770
                     /locus_tag="VXM85_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1346028.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8767..10044
                     /gene="tilS"
                     /locus_tag="VXM85_001473"
     CDS             8767..10044
                     /gene="tilS"
                     /locus_tag="VXM85_001473"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1346029.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPKLEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKTQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10048..10590
                     /gene="hpt"
                     /locus_tag="VXM85_001474"
     CDS             10048..10590
                     /gene="hpt"
                     /locus_tag="VXM85_001474"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1346030.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEVDYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10606..12564
                     /gene="ftsH"
                     /locus_tag="VXM85_001475"
     CDS             10606..12564
                     /gene="ftsH"
                     /locus_tag="VXM85_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1346031.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNKARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         4440 a         1933 c         2640 g         3883 t
ORIGIN      
        1 gagctattag ttctaaaaat gccattccta ttttatcaac agtaaaaatt gacgtgacca
       61 atgaaggtat tactttaatt ggttcaaatg gtcaaatttc aattgaaaat tttatttctc
      121 aaaaaaatga agatgctggt ttgttaatta cttctttagg ttcgatcctt cttgaagctt
      181 ctttctttat caatgtagta tctagtttac ctgatgtaac tcttgatttt aaagaaattg
      241 aacaaaatca aattgtttta accagtggca aatcagaaat taccctaaaa ggaaaagata
      301 gcgaacaata tccacgaatc caagaaattt cagcaagcac tcctttaata cttgaaacaa
      361 aattactcaa gaaaattatt aatgaaacag cctttgctgc aagtacacaa gagagtcgtc
      421 cgattttaac aggtgtccac ttcgtattga gtcaacacaa agagttaaaa acagttgcaa
      481 cagactctca tcgcctaagc cagaaaaaat tgactcttga aaaaaatagt gatgattttg
      541 atgtcgtaat tcctagccgt tctctacgcg aattttcagc ggtatttaca gatgatatcg
      601 aaactgtaga gattttcttt gccaataatc aaatcctctt tagaagcgaa aatattagct
      661 tctatactcg tctcctagaa ggaaactatc ctgatacaga tcgcttgatt ccaacagact
      721 ttaacactac tattactttt aatgtggtaa acttacgcca gtcaatggag cgtgcccgtc
      781 ttttatcaag tgcgactcaa aatggtactg tgaaacttga aattaaggat ggggttgtta
      841 gcgcccatgt tcactctcca gaagttggta aagtaaacga agaaatcgat actgatcagg
      901 ttactggtga agatttgacc attagtttca acccaactta cttgattgat tctcttaaag
      961 ctttaaatag cgaaaaggtg actattagct ttatctcagc tgttcgtcca tttactcttg
     1021 tgccagcaga tactgacgaa gacttcatgc agctcattac accagttcgt acaaattaag
     1081 tgaaagaggt tgagcctggc tcgcctcttt tatgatataa tcgaaaaaga aaaggagagt
     1141 agtatgtatc aagttggaaa ttttgttgag atgaaaaaat cacacgcttg tacaatcaag
     1201 tcgactggta aaaaggctaa tcgttgggaa attacacgtg taggagcaga tatcaaaata
     1261 aaatgcagta attgtgagca tgttgtcatg atggggcgat atgattttga gcgaaaaatg
     1321 aataaaatta ttgactgaga acccttagtt agagggttag cactttatcc ctttttgtgt
     1381 tataatatta gggattgaaa tgaaaacgga gaatgagaaa tatggctttg acagcaggta
     1441 tcgttggttt gccaaacgtt ggtaaatcaa cactatttaa tgcaattaca aaagcaggag
     1501 cagaggcagc aaactaccca tttgcgacta ttgatccaaa tgttggaatg gtggaagttc
     1561 cagatgaacg cctacaaaaa ctaactgaaa tgataactcc taaaaagaca gttcccacaa
     1621 catttgaatt tacggatatt gcagggattg taaaaggagc ttcaaaagga gaagggctag
     1681 ggaataaatt cttggccaat attcgtgaag tagatgcgat tgttcacgta gttcgtgctt
     1741 ttgatgatga aaatgtgatg cgcgagcaag gacgtgaaga cgcctttgtg gatccacttg
     1801 cagatattga caccattaac ctagaattga ttcttgcaga cttagaatca gtcaataaac
     1861 gatatgcgcg tgtagaaaag atggcacgta cgcaaaaaga taaagaatca gtagcagaat
     1921 tcaatgttct tcaaaagatt aaaccagtcc tagaagacgg gaaatcagct cgtaccattg
     1981 aatttacaga tgaggaacaa aaggttgtca aaggtctttt ccttttgacg actaaaccag
     2041 ttctttatgt agctaatgtg gacgaggatg tggtttcaga acctgactct atcgactatg
     2101 tcaaacaaat tcgtgaattt gcagcgacag aaaatgctga agtagtcgtt atttctgcgc
     2161 gtgctgagga agaaatttct gaattggatg atgaagataa aaaagagttt cttgaagcca
     2221 ttggtttgat agaatcaggt gtagataagt tgacgcgtgc agcttaccac ttgcttggat
     2281 tgggaactta cttcacagct ggtgaaaaag aagttcgcgc ttggactttc aaacgtggta
     2341 tgaaggctcc tcaagcagct ggtattatcc actcagactt tgaaaaaggc tttattcgtg
     2401 cagtaaccat gtcatatgag gatctagtga aatacgaatc tgaaaaggcc gtaaaagaag
     2461 ctggacgctt gcgtgaagaa ggaaaagaat atatcgttca agatggcgat atcatggaat
     2521 tccgctttaa tgtctaaaaa ttaataaatg gtgtcaatta ggttggaaaa aaattccaac
     2581 ccttttggct tttgaaagga aaaataaatg accaaattac ttgtaggctt gggaaatcca
     2641 ggggataaat attttgaaac aaaacacaat gttggtttta tgttgattga tcaactagcg
     2701 aagaaacaga atgtcacttt tacacacgat aagatattta aagctgacct agcatccttt
     2761 ttcctaaatg gagaaaaaat ttatctggtt aaaccaacga cctttatgaa tgaaagtgga
     2821 aaagcagttc atgctttatt aacttactat ggtttggata ttgacgattt acttatcatt
     2881 tacgatgatc ttgacatgga agttgggaaa attcgtttaa gagcaaaagg ctcagcaggt
     2941 ggtcataatg gtatcaagtc tattattcaa catataggaa ctcaggtctt taaccgtgtt
     3001 aagattggaa ttggaagacc taaaaatggt atgtcagttg ttcatcatgt tttgagtaag
     3061 tttgacaggg atgattatat cggtatttta cagtctgttg acaaagttga cgattctgta
     3121 aactactatt tacaagagaa aaattttgag aaaacaatgc agaggtataa cggataaatg
     3181 gtgaccttat tagatttatt ctcagaaaat gatcagatta aaaaatggca tcaaaattta
     3241 actgataaga aaagacaact aatacttggt ttatcaacat ctactaaggc tcttgcaatt
     3301 gcaagcagtt tagaaaaaga agataggatt gtgttattga cgtcaactta tggagaagca
     3361 gaaggacttg ttagtgatct tatatctatc ttgggtgagg aactcgtcta tccatttttg
     3421 gtagatgatg ctcctatggt ggagtttttg atgtcttcac aggaaaaaat tatttcacgg
     3481 gttgaagcct tgcgtttttt gactgattca tctaagaaag ggattttagt ttgtaatatc
     3541 gcagcaagtc gattgatttt accgtctccc aatgcattca aagatagtat tgtaaaaatc
     3601 tcagttggtg aagaatatga tcaacacgcg tttatccatc agttaaagga aaatggctat
     3661 cgaaaagtta ctcaagtaca aactcagggc gaatttagtc ttcgaggaga tattttagat
     3721 atttttgaaa tatcccagtt agaaccttgt cgaattgagt tttttggtga tgaaattgat
     3781 ggtatcaggt catttgaagt agaaacacaa ttatcgaaag aaaataagac agaactcact
     3841 atctttccag ctagtgatat gcttttgaga gaaaaggatt atcaacgagg acagtcagct
     3901 ttagaaaaac aaatttcaaa aactttatca cctattttga aatcatacct agaagaaatt
     3961 ctttcaagtt ttcaccaaaa acaaagtcat gcagactctc ggaagttttt atctttgtgc
     4021 tatgataaga catggactgt ctttgattat attgaaaaag atactccaat attctttgat
     4081 gattatcaaa aattgatgaa tcagtatgaa gtctttgaaa gagacttagc gcagtacttt
     4141 acagaagaat tacagaatag taaagcattt tctgatatgc agtatttttc tgatattgaa
     4201 caaatctata aaaaacaaag tccagtgacc tttttctcta atcttcaaaa gggtttagga
     4261 aatctcaaat ttgacaaaat ttatcaattc aatcaatatc ctatgcagga atttttcaat
     4321 cagttttctt ttctaaaaga agaaattgaa cgatataaaa aaatggatta caccattatt
     4381 ctgcagtcta gcaattcaat gggaagtaaa acattggagg atatgttaga ggaatatcag
     4441 attaaattgg attctagaga taagacaaat atctgtaaag aatctgtaaa cttaatagag
     4501 ggtaatctca gacatggttt tcattttgta gatgaaaaga ttttattgat aactgaacat
     4561 gagatttttc aaaagaaatt aaagcgtcgt tttcgaagac aacatgtttc aaatgcagag
     4621 agattaaaag attacaatga acttgaaaaa ggggactatg ttgtccatca tatccatggg
     4681 attggtcaat atctaggaat tgaaaccatt gaaatcaagg gaattcatcg cgattatgtc
     4741 agtgtccaat accaaaatgg tgatcaaatt tctatccccg tggaacagat tcatctactg
     4801 tccaaatata tttcaagtga tggtaaagct ccaaaactca ataaattaaa tgacggtcat
     4861 tttaaaaagg ccaagcaaaa ggttaagaac caggtagagg atatagctga tgatttaatc
     4921 aaactctact ctgaacgtag tcagttgaag ggttttgctt tctcagctga tgatgatgat
     4981 caagatgcct ttgatgatgc tttcccttat gttgaaacgg atgatcaact tcgtagtatt
     5041 gaggaaatca aaagggatat gcaggcttct cagccaatgg atcgactttt agttggggat
     5101 gttggttttg gaaagactga agttgctatg cgtgcagcct ttaaagcagt caatgatcac
     5161 aaacaggttg tcattctagt tccgacgacg gttttagcgc aacagcacta tacgaatttt
     5221 aaggaacgat tccaaaattt tgcagttaat gttgatgtgt tgagtcgctt tagaagtaaa
     5281 aaagagcaga ctgcaacact tgaaaaattg aaaaacggtc aagtcgatat tttgattgga
     5341 acacatcgtg ttttgtcaaa agatgttgtg tttgctgatt tgggcttgat gattattgat
     5401 gaggaacagc gatttggtgt caagcataag gaaactttga aagaactgaa gaaacaagtg
     5461 gatgtcctaa ccttgaccgc tacgccaatc cctcgtaccc tccatatgtc tatgctggga
     5521 atcagagatt tgtctgttat tgaaactccg ccgactaatc gctatcctgt tcagacctat
     5581 gttttggaaa agaatgatag tgtcattcgt gatgctgtct tgcgtgaaat ggagcgtgga
     5641 ggtcaagttt actatcttta caacaaagtt gacacaattg ttcagaaggt ttcagaatta
     5701 caggagttga ttccggaggc ttcgattgga tatgttcatg gtcgaatgag tgaagtccag
     5761 ttggaaaata ctctattaga ctttattgag ggacaatacg atatcttggt gacgactact
     5821 attattgaga caggggtgga cattccaaat gctaatactt tatttattga aaatgcggac
     5881 catatgggct tgtcaacctt atatcagtta agaggaagag tcggtcgtag taatcgtatt
     5941 gcttatgctt atctcatgta tcgtccagaa aaatcaatca gtgaagtctc tgaaaagaga
     6001 ttagaagcga ttaaaggatt tacagaattg ggctctggct ttaagattgc aatgcgagat
     6061 ctttcgattc gtggagcagg aaatctttta ggaaaatccc agtctggttt cattgattct
     6121 gttggttttg aattgtattc gcagttatta gaggaagcta ttgctaaacg aaacggtaat
     6181 gctaacgcta acacaagaac caaagggaat gctgagttga ttttgcaaat tgatgcctat
     6241 cttcctgata cttatatttc tgatcaacga cataagattg aaatttacaa gaaaattcgt
     6301 caaattgaca accgtgtcaa ttatgaagag ttacaagagg agttgataga ccgttttgga
     6361 gaatacccag atgtagtagc ctatctttta gagattggtt tggtcaaatc atacttggac
     6421 aaggtctttg ttcaacgtgt ggaaagaaaa gataataaaa ttacaattca atttgaaaaa
     6481 gtcactcaac gactgttttt agctcaagat tattttaaag ctttatccgt aacgaactta
     6541 aaagcaggca tcgctgagaa taagggatta atggagcttg tatttgatgt ccaaaataag
     6601 aaagattatg aaattttaga aggtctgctg atttttggag aaagtttatt agagataaaa
     6661 gagtttaagg aagaaaattc catttgatat ttttcttcta taaaatagat aaaaatagat
     6721 aaaaatggta caataataaa ttgaggtaat aaggatgaga ttagataaat atttaaaagt
     6781 atcgcgaatt atcaagcgtc gtacagtcgc aaaggaagta gcagataaag gtagaatcaa
     6841 ggttaatgga atcttggcca aaagttcaac ggacttgaaa gttaatgacc aagttgaaat
     6901 tcgctttggc aataagttgc tgcttgtaaa agtactagag atgaaagata gtacaaaaaa
     6961 agaagatgca gcaggaatgt atgaaattat cagtgaaaca cgggtagaag aaaatgtcta
     7021 aaaatattgt acaattgaat aattctttta ttcaaaatga ataccaacgt cgtcgctacc
     7081 tgatgaaaga acgacaaaaa cggaatcgtt ttatgggggg ggtattgatt ttgattatgc
     7141 tattatttat cttgccaact tttaatttgg cgcagagtta tcagcaatta ctccaaagac
     7201 gtcagcaatt agcagacttg caaactcagt atcaaacttt gagtgatgaa aaggataagg
     7261 aaacagcatt tgctaccaag ttgaaagatg aagattatgc tgctaaatat acacgagcga
     7321 agtactatta ttctaagtcg agggaaaaag tttatacgat tcctgacttg cttcaaaggt
     7381 gataaaatgg aaaatttatt agacgtaata gagcaatttt tgagtttgtc agatgaaaag
     7441 ctggaagaat tgactgataa aaatcaatta ttgcgtttac aagaagaaaa ggaaaggaag
     7501 aatgcgtaaa ttcttaatta ttttgttgct accaagtttt ttgaccattt caaaagtcgt
     7561 tagcacagaa aaagaagtcg tctatacttc gaaagaaatt tattaccttt cacaatctga
     7621 ctttggtatt tattttagag aaaaattaag ttctcccatg gtttatggag aggttcctgt
     7681 ttatgcgaat gaagatttag tagtggaatc tgggaaattg actcccaaaa caagttttca
     7741 aataaccgag tggcgcttaa ataaacaagg aattccagta tttaagctat caaatcatca
     7801 atttatagct gcggacaaac gatttttata tgatcaatca gaggtaactc caacaataaa
     7861 aaaagcatgg ttagaatctg actttaaact gtacaatagt ccttatgatt taaaagaagt
     7921 gaaatcatcc ttatcagctt attcgcaagt atcaatcgac aagaccatgt ttgtagaagg
     7981 aagagaattt ctacatattg atcaggctgg atgggtagct aaagaatcaa cttctgaaga
     8041 agataatcgg atgagtaaag ttcaagaaat gttatctgaa aaatatcaga aagattcttt
     8101 ctctatttat gttaagcaac tgactactgg aaaagaagct ggtatcaatc aagatgaaaa
     8161 gatgtatgca gccagcgttt tgaaactctc ctatctctat tatacgcaag aaaaaataaa
     8221 tgagggtctt tatcagttag atacgactgt aaaatacgta tctgcagtca atgattttcc
     8281 aggttcttat aaaccagagg gaagtggtag tcttcctaaa aaagaagata ataaagaata
     8341 ttctttaaag gatttaatta cgaaagtatc aaaagaatct gataatgtag ctcataatct
     8401 attgggatat tacatttcaa accaatctga tgccacattc aaatccaaga tgtctgccat
     8461 tatgggagat gattgggatc caaaagaaaa attgatttct tctaagatgg ccgggaagtt
     8521 tatggaagct atttataatc aaaatggatt tgtgctagag tctttgacta aaacagattt
     8581 tgatagtcag cgaattgcca aaggtgtttc tgttaaagta gctcataaaa ttggagatgc
     8641 ggatgaattt aagcatgata cgggtgttgt ctatgcagat tctccattta ttctttctat
     8701 tttcactaag aattctgatt atgatacgat ttctaagata gccaaggatg tttatgaggt
     8761 tctaaaatga gggaaccaga ttttttaaat cattttctca agaagggata tttcaaaaag
     8821 catgctaagg cggttctagc tctttctggt ggattagatt ctatgtttct atttaaggta
     8881 ttgtctactt atcaaaaaga gttagagatt gaattgattc tggctcatgt gaatcataag
     8941 cagagaattg aatcagattg ggaagaaaag gaattaagga agttggctgc tgaagcagag
     9001 cttcctattt atatcagcaa tttttcagga gaattttcag aagcgcgtgc acgaaatttt
     9061 cgttatgatt tttttcaaga ggtcatgaaa aagacaggtg cgacagcttt agtcactgcc
     9121 caccatgctg atgatcaggt ggaaacgatt ttgatgcgct tgattcgagg aactcgcttg
     9181 cgctatctat caggaattaa ggagaagcaa gtagtcggag agatagaaat cattcgtccc
     9241 ttcttgcatt ttcagaaaaa agactttcca tcaatttttc actttgaaga tacatcaaat
     9301 caggagaatc attattttcg aaatcgtatt cgaaattctt acttaccaaa attggaaaaa
     9361 gaaaatcctc gatttaggga tgcaatctta ggcattggca atgaaatttt agattatgat
     9421 ttggcaatag ctgaattatc taacaatatt aatgtggaag atttacagca gttattttct
     9481 tactctgagt ctacacaaag agttttactt caaacttatc tgaatcgttt tccagatttg
     9541 aatcttacaa aaactcagtt tgctgaagtt cagcagattt taaaatctaa aagccagtat
     9601 cgtcatccga ttaaaaatgg ctatgaattg ataaaagagt accaacagtt tcagatttgt
     9661 aaaatcagtc cgcaggctga tgaaaaggaa gatgaacttg tgttacacta tcaaaatcag
     9721 gtagcttatc aaggatattt attttccttt ggacttccat tagaaggtga attaattcaa
     9781 caaatacctg tttcacgtga aacatccata cacattcgtc atcgaaaaac aggagatgtt
     9841 ttgattcaaa atgggcatag aaaaaaactc agacgtttat ttattgattt gaaaatccct
     9901 atggaaaaga gaaactctgc tcttattatt gagcaatttg gtgaaattgt ctcaattttg
     9961 ggaattgcga ccaataattt gagtaaaaaa acgaaaaatg atataatgaa cactgtactt
    10021 tatatagaaa aaatagatag gtaaaaaatg ttagaaaacg atattaaaaa agtcctcgtt
    10081 tcacacgatg aaattacaga agcagctaaa aaactaggtg ctcaattaac taaagactat
    10141 gcaggaaaaa atccaatctt agttgggatt ttaaaaggat ctattccttt tatggctgaa
    10201 ttggtcaaac atattgatac acatattgaa atggacttca tgatggtttc tagctaccat
    10261 ggtggaacag caagtagtgg tgttatcaat attaaacaag atgtgactca agatatcaaa
    10321 ggaagacatg ttctatttgt agaagatatc attgatacag gtcaaacttt gaagaatttg
    10381 cgagatatgt ttaaagaaag agaagcagct tctgttaaaa ttgcaacctt gttggataaa
    10441 ccagaaggac gtgttgtaga aattgaggta gactatacct gctttactat cccaaatgag
    10501 tttgtagtag gttatggttt agactacaaa gaaaattatc gtaatcttcc ttatattgga
    10561 gtattgaaag aggaagtgta ttcaaattag aaagaataat ctttaatgaa aaaacaaaat
    10621 aatggtttaa ttaaaaatcc ttttctatgg ttattattta tctttttcct tgtgacagga
    10681 ttccagtatt tctattctgg gaataactca ggaggaagtc agcaaatcaa ctatactgag
    10741 ttggtacaag aaattaccga tggtaatgta aaagaattaa cttaccaacc aaatggtagt
    10801 gttatcgaag tttctggtgt ctataaaaat cctaaaacaa gtaaagaagg aacaggtatt
    10861 cagtttttca cgccatctgt tactaaggta gagaaattta ccagcactat tcttcctgca
    10921 gatactaccg tatcagaatt gcaaaaactt gctactgacc ataaagcaga agtaactgtt
    10981 aagcatgaaa gttcaagtgg tatatggatt aatctactcg tatccattgt gccatttgga
    11041 attctattct tcttcctatt ctctatgatg ggaaatatgg gaggaggcaa tggccgtaat
    11101 ccaatgagtt ttggacgtag taaggctaaa gcagcaaata aagaagatat taaagtaaga
    11161 ttttcagatg ttgctggagc tgaggaagaa aaacaagaac tagttgaagt tgttgagttc
    11221 ttaaaagatc caaaacgatt cacaaaactt ggagcccgta ttccagcagg tgttcttttg
    11281 gagggacctc cggggacagg taagactttg cttgctaagg cagtcgctgg agaagcaggt
    11341 gttccattct ttagtatctc aggttctgac tttgtagaaa tgtttgtcgg agttggagct
    11401 agtcgtgttc gctctctttt tgaggatgcc aaaaaagcag caccagctat catctttatc
    11461 gatgaaattg atgctgttgg acgtcaacgt ggagttggtc tcggcggagg taatgacgaa
    11521 cgtgaacaaa ccttgaacca acttttgatt gagatggatg gttttgaggg aaatgaaggg
    11581 attatcgtca tcgctgcgac aaaccgttca gatgtacttg atcctgccct tttgcgtcca
    11641 ggacgttttg atagaaaagt attggttggc cgtcctgatg ttaaaggtcg tgaagcaatc
    11701 ttgaaagttc acgctaagaa caagccttta gcagaagatg ttgatttgaa attagtggct
    11761 caacaaactc caggctttgt tggtgctgat ttagagaatg tcttgaatga agcagcttta
    11821 gttgctgctc gtcgcaataa atcgataatt gatgcttcag atattgatga agcagaagat
    11881 agagttattg ctggaccttc taagaaagat aagacagttt cacaaaaaga acgagaattg
    11941 gttgcttacc atgaggcagg acataccatt gttggtctag tcttgtcgaa tgctcgcgtt
    12001 gtccataagg ttacaattgt accacgcggc cgtgcaggcg gatacatgat tgcacttcct
    12061 aaagaggatc aaatgcttct atctaaagaa gatatgaaag agcaattggc tggcttaatg
    12121 ggtggacgtg tagctgaaga aattatcttt aatgtccaaa ctacaggagc ttcaaacgac
    12181 tttgaacaag cgacacaaat ggcacgtgca atggttacag agtacggtat gagtgaaaaa
    12241 cttggcccag tacaatatga aggaaaccat gctatgtttg gtgcacagaa tcctcaaaaa
    12301 tcaatttcag aacaaacagc ttatgaaatt gatgaagagg ttcgttcatt attaaataag
    12361 gcacgaaata aagctgctga aattattcag tcaaatcgtg aaactcacaa gttaattgca
    12421 gaagcattat tgaaatacga aacattggat agtacacaaa ttaaagctct ttacgaaaca
    12481 ggaaagatgc ctgaagcagt agaagaggaa tctcatgcac tatcctatga tgaagtaaag
    12541 tcaaaaatga atgacgaaaa ataaccctga gagaggctgg agcctctctt ttttgtgcag
    12601 tttaggagct aaagggaaca gaatggagaa aatggaacaa atgtgttttc taatctgtta
    12661 gactgtatct agaaagggga aaattatgat taaagaattg tatgaagaag tccaagggac
    12721 tgtgtataag tgtagaaatg aatattacct tcatttatgg gaattatcgg attgggacca
    12781 agaaggcatg ctctgcttac atgaattgat tagtagagaa gaaggactgg tagacgatat
    12841 tccacgttta aggaaatatt tcaaaaccaa gtttcgaaat cgaattttag actata
//
LOCUS       DAVLRT010000052        12780 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLRT010000052 DAVLRT010000000
VERSION     DAVLRT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.052"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..303)
                     /locus_tag="VXM85_001476"
     CDS             complement(<1..303)
                     /locus_tag="VXM85_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346032.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDISSEEYQFAKNNLDVQKNLLKRKTEILTL"
     gene            complement(307..894)
                     /locus_tag="VXM85_001477"
     CDS             complement(307..894)
                     /locus_tag="VXM85_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346033.1"
                     /translation="MLRLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            complement(926..1150)
                     /locus_tag="VXM85_001478"
     CDS             complement(926..1150)
                     /locus_tag="VXM85_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346034.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYYSYSEYPVGSGWNYARYEVINYYTGGY"
     gene            complement(1292..3532)
                     /locus_tag="VXM85_001479"
     CDS             complement(1292..3532)
                     /locus_tag="VXM85_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1346035.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKAAQHGSKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGWNSW
                     KIESVR"
     gene            complement(3516..4166)
                     /locus_tag="VXM85_001480"
     CDS             complement(3516..4166)
                     /locus_tag="VXM85_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV1346036.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     VEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            complement(4234..4803)
                     /locus_tag="VXM85_001481"
     CDS             complement(4234..4803)
                     /locus_tag="VXM85_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1346037.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLVNPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            4980..5405
                     /locus_tag="VXM85_001482"
     CDS             4980..5405
                     /locus_tag="VXM85_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1346038.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDVLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            5406..5819
                     /locus_tag="VXM85_001483"
     CDS             5406..5819
                     /locus_tag="VXM85_001483"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV1346039.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            5765..6091
                     /locus_tag="VXM85_001484"
     CDS             5765..6091
                     /locus_tag="VXM85_001484"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346040.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(6458..6717)
                     /locus_tag="VXM85_001485"
                     /pseudo
     CDS             complement(6458..6717)
                     /locus_tag="VXM85_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6720..7220)
                     /locus_tag="VXM85_001486"
     CDS             complement(6720..7220)
                     /locus_tag="VXM85_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1346041.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGFFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            7256..7435
                     /locus_tag="VXM85_001487"
     CDS             7256..7435
                     /locus_tag="VXM85_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346042.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            complement(7413..8381)
                     /locus_tag="VXM85_001488"
     CDS             complement(7413..8381)
                     /locus_tag="VXM85_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1346043.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(8467..8682)
                     /locus_tag="VXM85_001489"
     CDS             complement(8467..8682)
                     /locus_tag="VXM85_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1346044.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDAIWQEYLEH"
     gene            complement(8691..9545)
                     /gene="cvfB"
                     /locus_tag="VXM85_001490"
     CDS             complement(8691..9545)
                     /gene="cvfB"
                     /locus_tag="VXM85_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1346045.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(9605..10162)
                     /gene="frr"
                     /locus_tag="VXM85_001491"
     CDS             complement(9605..10162)
                     /gene="frr"
                     /locus_tag="VXM85_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1346046.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(10171..10908)
                     /locus_tag="VXM85_001492"
     CDS             complement(10171..10908)
                     /locus_tag="VXM85_001492"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1346047.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(10992..12326)
                     /gene="trmFO"
                     /locus_tag="VXM85_001493"
     CDS             complement(10992..12326)
                     /gene="trmFO"
                     /locus_tag="VXM85_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1346048.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(12571..12765)
                     /locus_tag="VXM85_001494"
     CDS             complement(12571..12765)
                     /locus_tag="VXM85_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1346049.1"
                     /translation="MEAEAIRRNCERGAIFSGKIKYHEDSQFKGDHYVECYAVLDNTV
                     IARDRITVPIDPLCGKDFIE"
BASE COUNT         3645 a         2921 c         2409 g         3805 t
ORIGIN      
        1 taaagtcaga atttctgtct ttcgcttcaa aagatttttt tgcacgtcta aattattttt
       61 agcaaactgg tattcctccg agctgatatc agacatttgg gagagtttct cttcattttc
      121 attgatagcc ctctcgttgg ctgcaatgcg actttccaac ctgctctcca agctgtgtga
      181 gtttgcagtc tgactattta aataaaaggt aacaccgagt acagatacaa ataaagctaa
      241 gacaatccag tttaagcggc ttttgaaaac ttttttcaat aaaaatagac taacatcttt
      301 cataaactaa acctcttcta tctgcccctg atgaatggtt actactctat cgcagacatc
      361 aaccaactct tccttatagt gggaacttaa aagaaccagc tgttcttgtc tatcgatttg
      421 tgctagccta tcaaaaaact tctgtcgata atactcatct aagccatttg taatctcatc
      481 catgagccag catttggcct gactgaggaa atacatggca atcaccaagc gttgcttcat
      541 gcctaaggaa tacttgcgaa tgggaagact gatatagtca gacatttccc aataggcgat
      601 ttcatccctc aagttcagac ctgacttcca gatgtttttg ataagacgaa ggtagtccat
      661 cccacttaag tttccatcca gccattcaat actctcataa taaaataaag aaggaggggc
      721 tgcgatattt ccactactta tgggaattaa attgctaatg gcacggaaca gggtcgtctt
      781 tccagagcca ttgatagcaa gaagaccata aaccctaccc tttttaaagg tgaaatccac
      841 atcttgcaag atgacttgtc gcgtttttag ggtaacatga gtaagcctta acatatccag
      901 ccctcctttt ctcactcttt aaggattaat aacctccagt atagtagttt atgacctcat
      961 aacgagcgta gttccagcca gaaccaactg gatactcaga atagctgtaa taacgagacc
     1021 atgacggaat ccaagcatac tgatggtcgt gatagttggt actatattcc caaaccgtat
     1081 tccaatcata cttgtaactt ttagtgactg tcacagcaga tatactggac tgaaaaagac
     1141 caataatcat aaactaacta attaaacgat ttttgctaat ttttaatgat tttatatcct
     1201 cagtattgaa tacattttca ttataccatt tttatcatta ttatgcaaca aatttttagt
     1261 ttatttcact aatctacaac atttatcctt cctatcgaac actttcgatt ttccaactat
     1321 tccaaccttt aaaccgtata gctccttgct ggtcagttcg gtaaactttg ctattgatac
     1381 cttccagtcg tgtcaatgtt tcctgatggg ggagtttcat tcgattgctc tttccaactg
     1441 agataagagt aagctctggt ttgagttttt ctagaaaggc tggacttgat gactttttag
     1501 agccatgttg ggcagctttc aaaacattta ctttcaagtc tggatagtgc ttcagcaagt
     1561 ccttctctcc tttctcctcc aaatttcccg tgaagagaaa ttgcttatcc aagaatttcc
     1621 catacagaac tagggtatca tcgtgtcctc catctcccat tttccttgga gatagaactt
     1681 ctaactgact tccaaaaatg ggcaagttct cccctactgt catactacgc acctttgttt
     1741 gagtcgcctg tagttctacc acaaattgct tctgcttcaa actgcctttt gatactaaaa
     1801 tctcccctac atggaaagcc ttggtcacct ctaacaaatc tccgacatgt tccttgtccg
     1861 tatttgtcaa aatcagctgg tcaatcttag ctactcctcg acttttaaga tagggtatca
     1921 agcttctctg tgcattgctg gtcgtcatct tttcttgcca tttttcgatt ttcttataag
     1981 attctgcctt accacctaca tctatgagaa tggttttccc agttacatcc cgtaggaaaa
     2041 tactttctcc ttgccccaca tccagcatgg tgatttcatt ttccagtgga tgcttggtca
     2101 ggagaaagag ccctgtaatc aataagcaca ataccgttag ctttttaatg ttttttctca
     2161 aatcatagac caaagccaag gaaattaaca ataggattaa aaaccatgca ttgggttgtc
     2221 caaagaccag aggtctactt gtcacctgtg acaccaagcg aataatgccc tccaaccatt
     2281 caaagataaa gttcagctga atgactggat agagaaagga aaggacaaat aaaatagaca
     2341 agagcggtaa gaaggtcaag tcaaatagaa aggaaaagac aaaggtcaaa aggatagacc
     2401 aaggttgaaa ttccgcaaaa tagaaggata gaatgggcaa tatgcccaag gagatgacta
     2461 gactttcact agtaacagcc ttgagcccct ccccttcttt gctggtcatg gtcaggataa
     2521 aagcataagc gcaggacaag actcctcctg ctgtcaagaa aaagtttggc atgacaataa
     2581 agaggacaag caccgtcaag gcaaaattat ccaagccctt aaccccatgt tgagccagta
     2641 gcttttgcaa gagactgcga ataaccgatg ctgaaaagcc agttagtccc gcatagataa
     2701 gggaaaaggg ataagtcagc catttcaact tttcttgggt caagcccaat cgcaagagaa
     2761 gtttcttaaa tccattcatg aaaaaaccta cctgcatacc agatagagca aagaggtgga
     2821 taattcctag actggaataa agctcattca tctcctcaaa gtcggtgtcc agatgtccca
     2881 gcaagagtcc tgtcatgtaa ttgcgcatag ggtctggaaa gtgcgtctta atccaaacca
     2941 cagcctttcg acgtaaactg gacaagtttt ctcctatatc ccaactgcca atcttttgaa
     3001 gtgactggat tgttttgata ttgagagtct ggtaaattcc ctgagtcttc agataggctt
     3061 ggtaattaaa gccaccaaaa tttctctgcc cttctggctc cgaaagcttc ccttctagtc
     3121 ctatctcatg caggtcggtt aaagcttgaa aggcttcttt ctcctcctcg gactggagtt
     3181 tataatagac ttggaaagca cgaccgttag acttgccacg aaaggataga ctatcaccat
     3241 taaccttaat agtatcaggc aaaatccgta ccctttcaac agaatccgcc agattttgac
     3301 tcgcttgact ctgttgccaa ttttgaaaaa caaaccaaaa tccaaagatt ccgcaaatta
     3361 ttagaacttt accagcagat ttccacggaa attggataaa gagacagact agcagaaaaa
     3421 caaagcccaa caaagcaaga taagatgctg agaaaatagc gtaataaagc caaagtaata
     3481 gaaaactcag gtaaattagg ggaatagaga aattcttaat ccactgtaac atagtcttta
     3541 agcttttcta ttgttttgcc accaatgcca gagaccttct tgagctcgtc tactgacttg
     3601 aacttgccat ttgcctcacg atggtcgata atgtcctgag ctcgttttcc tcccagtccc
     3661 ttgacctgct tgagttcttc cagactggcc ttgttgagat tgaccttctt ttccttgctt
     3721 gttgaagaag ctgtccccga accagtctgt tgactaactg cttcttctcc cttagtagga
     3781 acgtaaacca gagcctcatc actaactttc tgagctagat tgagcgactt gctgtctgct
     3841 tgctctgtca agccaccagc cttctgaaca gcatcattga ctcgactacc tacaggcaag
     3901 tcataaatcc ctggcgattt gacagcacct ttgacatcta ctgtgattag atcttgttca
     3961 actggttctt ccttctcttc cttgttcact tccttttcgg ataccaaatc cttggaaaca
     4021 gctgcaactt caacctgcaa attcgtttct ttgacaggtg tttgtggagc tggctttagc
     4081 aggaaaaatc cgcctacaag caagcccaga ccagtacaga tgacgatgat tttatactct
     4141 ttgattttct cgataattgc ttccatattt tctcctctct tagattattc gtaagaggaa
     4201 gaaaaaacag tcgaaaaatt tcttttcaac tgcttattta tttgcaaaat agtcttcctt
     4261 ggtcaagaca taatgaactc ttgtcacgat tcggcctttt tcatgctggt ccatacaagc
     4321 atatggttct gcatgggaaa aacgcatgcc tgatttctcc atgacctttc ctgacgcggg
     4381 attagcctta tcgtgaaggg cagtcaactt attcatccct atcttctcaa aagctagctc
     4441 aatcacagca cgattggctt ctgtcgttaa tccttgattc caatactttt tattgataat
     4501 gtagccaata gctgccttct taagaacaga atcaatcttg tgcaagtcaa tggttccaat
     4561 aaactgacca ttgcttttta gttctattcc ccaacgtccc aagggattaa ccaaatagaa
     4621 ctgagcaatg ttattcttgg tttcttccaa gctttgattg gttggaaaag tgtaacgtgt
     4681 attacccttg tccgaggcat agtcaaacat tgcttccgca tcatccaaag ttacaggtct
     4741 aagcaataaa cgctccgttt ctatagacgg atactgggca agttttacaa aaattgattc
     4801 catacttttc cctccattag agcttgattt tcactagtct agcataaaaa aattgggatg
     4861 acaagttttt cttgattttt gaagcggttt catatacaat gaaaccgtca catttttgat
     4921 ttatgaactg gaatgtgaca accaaaacaa gaccgtcaat tgaaaggtag gttatctcaa
     4981 tgttaatcgg aatcccaaaa gaaattaaaa ataacgaaaa ccgtgtcgcc ctcacacctg
     5041 caggtgttca tagcttagtt agtcgtggtc atcgtgtcct tatcgaaaca aatgctggtc
     5101 tcggttctgg ctttactgat gctgactatc aaaagcaagg agctgagatt gtcgctactg
     5161 ctggtgaagc ttgggcagca gagttggttg tgaaagtaaa agaaccttta agttctgaat
     5221 acggttactt gcgcgacgtt cttcttctct tcacctactt gcacatggcc gctgctccag
     5281 aattagcaga tgctatgtta gcagcaaaaa caacaggaat tgcctatgaa actgttcgtg
     5341 acaatcaagg acaactaccg ctcctcgttc ctatgagtga ggttgcaggt cgtatggctg
     5401 tttaaatcgg agctcacttc cttactaagc aagctggtgg ctctggtgtt ctacctggtg
     5461 gtgtaccagg tgttccaaaa ggaaaagtaa ctatcatcgg tgatggtgtc gtcggtacac
     5521 atgctgcccg catcgccctt ggtcttggtg ctcaagtgac tattttagat attagtgcca
     5581 agcgtctctc agttctagaa gaagtctttg gaagtcaaat tcaaactctt atatctaatt
     5641 cattcaacat tgaagcaagt atgagagatg ctgatgtggt gattggagcc attctcatcc
     5701 ctggtgcaaa agcaccggaa ttggtgacag atgagatggt caaacaaatg cgtccaggct
     5761 ctgtatcgtt gacgttgctg ttgaccaagg tggcgttatc gaaacagctg accgtgtgac
     5821 aacgcacgat gaacccgtct atgaaaaaca cggtgttctc cactatgccg ttgccaatat
     5881 ccctggtgcg gttgctcgca cttcaaccat cgccctaacc aatgtcactc ttccttatat
     5941 cgaagctttg gctggcaaag gattcgcaca agcaatctct gaagatgaag gcttgcgtca
     6001 aggtgtgacc acttatcaag gttacttgac tagcctacca gttgctcaag gacttaatcg
     6061 tgactacact gatatcaatg atttagtata aattttgaaa agaaaagggt gacttcgcaa
     6121 gtcctaccca ctgtgctttg tgtcatcagc tattacatcg ttggtttatt gtactgtata
     6181 ctttgcaact ggagactttc gttccagact caattgtgca taagaaactc taaattgaat
     6241 aaagtcaaca gagtttcgcc caaccacttc gtcttgtaat gacaattata taaaccaaag
     6301 gaagctgagc tctttagtcc cagcttcttt ttatatataa aattttaccc gtgcaaagac
     6361 agggccttag cagacttctt ttttacttcg ttcacccttg ctttttcttt ttaggtttgg
     6421 gcgttggcag ttggttatac atagctaaaa tcaggtctta tagaaacatc ttattatcaa
     6481 gttcttccac tcaaatcatt tctttggcac ctttgtatgg aaactcaaaa gaagattggt
     6541 caatcttatc taagactgct tgcacgggtt taactaaaag cgatcgtcat aaatgccgcc
     6601 aataatcttg ccgcggaagt aaagaatata ctcccccatc atggaacggt aagtcacatc
     6661 atctaatcct gataattgtt ccaaaacaaa ttccaaatag ttcttacttg atgccatttc
     6721 taatcttcta ggctctgttc aacgataaca accgtataga gttcttgctt aacctcgcat
     6781 ccaattgatt taaagccctg cttttcccaa aaatgctgag attgcggatt tcccttaaca
     6841 taagccaaac gtgcctttcg aaagttctta gcaaaataag ctagtgcttc tgtcacaata
     6901 tgactaccaa tccctttcct ctgataggct tgatcaacca taaaaaaacc aataaaaaca
     6961 gtctcctcat caggatatgc atagacaaaa tccataacag ccacaaggtc agatccattc
     7021 caaaatccaa caaaaaactt atcagcctta gctttacctt caggtagaca aagcatgtcc
     7081 tcttttacag ttgcaaaatt tggctctggt ggacaatgct gaaaatacag aggattactt
     7141 tcatataaag ataaaatact tggaatatcc ttttcagtta gtatcctaca actgtaatac
     7201 ttagatagtt ggtcaatcat cttttcaaat tcctactttc ttgtgccctg tgattatgac
     7261 acaggaagat gcactgatcg tcatcagtca cataaaagtt ctttccatcg tgcctaatcg
     7321 ttgtctcaga cctttggata aaacctttag cctatacaac tggattttcc tctctcaaaa
     7381 gtatattctt ttgcaggcga acttcctcaa aatcagtcgt gtgcaacttc agtagaatat
     7441 tcataggctc ggataatctg agcgacaact ggatggcgaa ccacatcctt ggctgaaaaa
     7501 tgaacaaagt caatctgatg gatgttcttg agtttctctt gagcatcaat caaaccggac
     7561 ttgacattac gtggcaggtc aatctgactg atatctccat tgacaatcat cttagaatga
     7621 aagcctaaac gcgtcaagaa catcttcatc tgcatgatgg tcgtgttttg cgcctcatcg
     7681 agaatgacaa aggcatcatc caaggtccgg ccacgcatat aggcaagggg cgcaatttcg
     7741 ataatttcac gctccatgag acgagtcgtt tggtctttcc caagaatttg atacaaggca
     7801 tcgtaaacag gacgaaggta aggatccacc ttctccttaa gatcacccgg aagaaatcca
     7861 agactctctc ccgcttccac cgctggacga gttaggataa ttcgcttgac ttgcccacgt
     7921 ttaagggcag tcactgccaa ggtcactgca aggaaggtct tccctgtacc tgctggccca
     7981 attccaaagg tcacatcatg ctgtttgaca ctgtccacat aaagtttttg acctaaggtt
     8041 ttgacgcgga taggcttccc agtattatcc ttgatgattt cttcttcgta aagggcgaca
     8101 aacttgtcaa tttcatcatt tttgaccata ctaatagccg tcactacatc tggcgtacca
     8161 acggtcatcc cacgatttac caagaccatg agagcttgaa taacctgacg ggcttcctcg
     8221 caagcagact cttctcccaa aacctggaca atctccgtac gagcatgaat cacaacatca
     8281 agctcttctt ccatcaaacg aagatggcgt tcattggaac caaaaagatg aaacaggtca
     8341 tctggatgac tcagttgaat gtctattgaa tgttccttca aataaagaac ctctctaatc
     8401 cttttatttc tttttattat agcagaggga tggagaaatt actagcccaa acctagtgaa
     8461 taaatgctag tgttctaagt attcttgcca gatagcgtca aagtctccta ggttaaaaga
     8521 gaaactggca tgctcctcca aaaagcgact gacctcatca aaatcatctg tgtgttttgg
     8581 aaaagctgcc tcttcaaaag cgaggtctgc caaaattgct ttgggactgt tacttttagg
     8641 attgcgctcg gtcatgagcc aagtgtaaaa tgattttctc ataagcctcc ctaaatcaac
     8701 tctgtcccaa actggtcctg cttgatttta ccagccttca taagaccacc taaggctttc
     8761 ttgaactgac ctttagaaat gccaaaggtt gccttgatgt cgtctggaga tgacttatca
     8821 tttaaggtca tgaaaccgcc attactttcc aaataagtca aaatcatctg agcatcgttt
     8881 tccaacattt caaaggagcg tggtttgagg gagaggttca gagtgcggtc cacttcacgg
     8941 aaaccaataa cgcgcgcatc taatacttgc cccaaacgtg gctctgcgta acgctcgcta
     9001 ggatgaataa aaccaagcat attattttct ggtaggtaaa caaaagttcc tgacagcttg
     9061 agacggtaaa caatggctgg ccagttttgg ttctgcatgt tgttgtaggc aggacgagca
     9121 agacgttgga agtcttcttg ataagccaag aggccccaga tacggtcttt cttatccact
     9181 tcaagacgga tgtagagttg gtcgcccttc ttaggccaga gttccttgag ctcagggaga
     9241 atatcgagtg acacaacgat ttccttgtca ggaaggcctg tatccacaaa gacacccaag
     9301 tccttacgaa cctctgtgac acgtccccaa ccaaattggt cctgagtggc agtcacttct
     9361 aaggttgtca ggcggagttt ttgcttcata tccgtgtatg caaaaccctt gaccgtatcc
     9421 cctactgtat gttggccttc ttccttagca agagcatagg tttgaccatc cttttgcaca
     9481 aagtaaaaac ggtcgttttc atcgatgatc agtccaacga taaaacttgc aagatttgta
     9541 ttcatatttc cttctttcga ataaaactca gccagcaatg ccaactgagt ttttctgttt
     9601 atttttagac ttccaaaagt tctttctctt tgttagcagt catgtcgtcg atgtgtttaa
     9661 cagcatcgtc tgttactttt tgaatgtctt tttcaagagt cttcaattcg tcttcagtga
     9721 tttcttttgc tttttcttgt ttcttagctt cgtccatagc atcgcgacgg atattgcgga
     9781 cagccacttt agcattttcg ccgaccttct tcacttcttt agcaaggtca cgacgagttt
     9841 cttctgtaag agctgggata accaagcgaa tcacagaacc gtcattagcc ggtgtgatac
     9901 caagatcaga agcgttcaag gcacgttcga tgtctttcaa tgaagacttg tcaaatggtg
     9961 ttaccaacaa aacacgcgct tctggaatcg taattgaagc gatttggtta agaggagttt
    10021 cgactccata gtattctaca tgtacacggt caagcaagct tgcattggca cgaccagcac
    10081 ggataccacc aaattcacga gcaagtgatt ggtgagactg ggtcattctc tctttagctt
    10141 tttcaataat tacgttagcc atattctttc ttattccttt tcttcgatat tatttgaaac
    10201 tgttgttccg atattttcac caaatacgac acgtttgatg ttgcctgatt ggttcatgtt
    10261 gaagacaacc aagtcaatgt cgttgtccat tgagagggtt gaggctgttg agtccatgat
    10321 acgaagacct ttgttgataa catcacggtg ggtcaattct tcaaacttaa cggctgtctt
    10381 gtccttctta ggatcggcat tgtacacacc atcgacgcca tttttagcca tgaggatggc
    10441 atctgcttcg atttcagctg cacgaagggc cgctgttgta tctgtcgaga agtatggtga
    10501 accaattcca gcaccaaaga taacgatacg gcctttttca aggtgacgaa gggcacgtcc
    10561 acggacataa ggctctgcca cttgttgcat agcaatagct gtttgtacac gcgtatcaac
    10621 cccaacttgt tgcaatgaat ctgccatcac aagagcattc ataacagtcc caagcattcc
    10681 agtgtaatct gcctgaacac ggtccatacc tgcttctgct gcaggttctc cacgccagag
    10741 atttcctcca ccaataacaa gggcaatttc gatacctaag ctatgaactt cttgaatctc
    10801 ttttgcgatt gtttgaactg tttggatatc aatccctacg ccacgttcac cggcaagggc
    10861 ttcacctgat aacttgatta aaatacgttt atacttggga ttcgccattt tcactctcct
    10921 tctttcatcc tacctatttt atcacaattt ctaagatttt tatagtatca tgaacaattc
    10981 tttcaaaaaa attagacagt caaaaattcc tctaagtcgg caagggcacg ctctgcaatt
    11041 ttttcataac gagccttctt atcacggata cgctcgcctt ccaactcctt gatgatccca
    11101 aaattgacat tcattggttg gaaatgtttg ctgtcggcat gggtaatgta atgagctaag
    11161 cttccaatcg ctgtcgtctc ggggaaaata acctcgcttt cttccttgaa gagacgagct
    11221 gcgttaattc ccgcaactaa gcccgaagcc gccgactcaa catagccttc cacccccgtc
    11281 atctgaccag caaagaagag atttggttgt ttcttggaac ggtaagtctg ctcaagaaga
    11341 tttggtgaat ccatgtaaga attgcgatgc atgacaccat aacggacaaa ctccgcattt
    11401 tcaagacccg gaatcatttg gaagacacgc ttttgttctc cccatttgag gtgggtctgg
    11461 aaaccaacaa tattgtatag gctaccagct gcattatcct gacgaagttg cacaaccgca
    11521 taaggtgttt taaattctcc atcacgaggt cctgtgtagt cgtccggata ctcaagaccg
    11581 actggcttca tagggccata aagcatagtt ttaatgccac gtttggccat aacttcgatt
    11641 ggcatacatc cttcaaagta cttttctttt tcaaaagaat tgagcggtgc ttcttccgca
    11701 ttgaccaagg cttcatggaa atccataaac tcttgcttgg tcattggagc attgagatag
    11761 gctgcttctc ccttatcata acgagatttg agatagacct tactcatatc gatagtgttg
    11821 acatcgataa taggcgctgc tgcatcgtag aaatagaagc catcgccatt attaagggca
    11881 tgaatctttt cagccagggc atcgctagtc aagggaccag tcgccacaac tgtaataaca
    11941 tctgttggca attctgtaat ttcatcacgc accacttcaa tcaaggggtg gttgaccact
    12001 ttttcggtca ccatttgaga gaaaccatca cgatccaccg caagggctcc accggcagga
    12061 acacgtgtag cctcagcaga ttccaagata acagaaccca agcgacgcat ttcttccttg
    12121 agaagaccaa ctgcatttgt caaagcatcc ccacgcaaag aattggaaca aaccaactca
    12181 gcaaaattgt ctgttttatg ctggggtgta gacttgacac cacgcatttc atatagttta
    12241 actggaatac cacgctctgc gatttggtaa gctgcttcag aacctgccaa accagcaccg
    12301 ataacattga tataagattg agacacgaca ctaatacctc tttgggagtg tgaagttaag
    12361 attcacattg aaaaagccaa tcagacttac aagctttcga gtttcttggc tcaggctgaa
    12421 aaagtccaca ggactttttc actcccacaa atctttctat tttttcttct actagtataa
    12481 caaaaaaagg gaagaaggca aacttccctg tttagtcatt ttcttgatgt aaagagatag
    12541 tgagtattcc agttaagaat caattatatg ctactctata aaatcttttc cacataacgg
    12601 atcgataggg actgttattc tatctcttgc tataaccgta ttatctaaaa cagcataaca
    12661 ttcaacatag tgatctcctt taaactgtga atcttcgtga tattttattt tacctgaaaa
    12721 aatagcacca cgctcacaat ttcttctaat agcctcagct tcaatatttc ttactttcca
//
LOCUS       DAVLRT010000053        12054 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLRT010000053 DAVLRT010000000
VERSION     DAVLRT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.053"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..128)
                     /locus_tag="VXM85_001495"
     CDS             complement(<1..128)
                     /locus_tag="VXM85_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV1346050.1"
                     /translation="MNDEASKQLTDARFKCLVGVQRTTFEEMLAVLKTAYQLKHTKG"
     gene            271..582
                     /locus_tag="VXM85_001496"
     CDS             271..582
                     /locus_tag="VXM85_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346051.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            586..1302
                     /locus_tag="VXM85_001497"
                     /pseudo
     CDS             586..1302
                     /locus_tag="VXM85_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1432..2247)
                     /locus_tag="VXM85_001498"
     CDS             complement(1432..2247)
                     /locus_tag="VXM85_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1346052.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2244..3149)
                     /locus_tag="VXM85_001499"
     CDS             complement(2244..3149)
                     /locus_tag="VXM85_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1346053.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            complement(3524..3607)
                     /locus_tag="VXM85_001500"
                     /pseudo
     CDS             complement(3524..3607)
                     /locus_tag="VXM85_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            3651..5780
                     /locus_tag="VXM85_001501"
     CDS             3651..5780
                     /locus_tag="VXM85_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1346054.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIADLEKEAEKFVCEGFATGKEALAGAVDI
                     LVEALSEDVTLRAMTYQEVLRHSKLTSQVKDESLDEKQVFQIYYNFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNINTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKQIDTEREKVNLSLLAPNETD"
     gene            5767..6216
                     /locus_tag="VXM85_001502"
     CDS             5767..6216
                     /locus_tag="VXM85_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1346055.1"
                     /translation="MKLTDYVKQVSLEDFGRSFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTRRYRCGLCRGKLLLINQPED"
     gene            6275..6547
                     /locus_tag="VXM85_001503"
     CDS             6275..6547
                     /locus_tag="VXM85_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1346056.1"
                     /translation="MNSKFYKMRRNRMISGVLVGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(6595..6759)
                     /locus_tag="VXM85_001504"
     CDS             complement(6595..6759)
                     /locus_tag="VXM85_001504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346057.1"
                     /translation="MDEAGEILLILFKNQSANYVNFTLPTKLQPAAGFLVCTLIFIEY
                     KSKKALNQAV"
     gene            complement(6901..7092)
                     /locus_tag="VXM85_001505"
     CDS             complement(6901..7092)
                     /locus_tag="VXM85_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346058.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            7520..8278
                     /locus_tag="VXM85_001506"
     CDS             7520..8278
                     /locus_tag="VXM85_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346059.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            8280..10268
                     /locus_tag="VXM85_001507"
     CDS             8280..10268
                     /locus_tag="VXM85_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1346060.1"
                     /translation="MFRLTNKLAISNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKKLGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTIFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLNQFPDSDIYNQFYGGMNVSISEEEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSVLFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQIINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(10310..10954)
                     /locus_tag="VXM85_001508"
     CDS             complement(10310..10954)
                     /locus_tag="VXM85_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1346061.1"
                     /translation="MLRAGVLDANNGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKAPARTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(10980..11855)
                     /locus_tag="VXM85_001509"
     CDS             complement(10980..11855)
                     /locus_tag="VXM85_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1346062.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNCKRSVKKSSVTKELKEKILHYHNQ
                     KFSPDKKQASINFKPAGQSIEQRPEAINFRLENGHYEIDTVLLTRAKNYCLLVLTDRK
                     SRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHP
                     YASWERGTNENRNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKYLDYKSPREDFLM
                     ANLNLKFSVRNKSRN"
BASE COUNT         3678 a         2274 c         2502 g         3600 t
ORIGIN      
        1 ccttttgtgt gtttaagttg ataagcagtt tttaatacag ctaacatctc ttcaaaagtg
       61 gtgcgctgaa caccaacaag acatttaaat cgtgcatcag ttagttgctt acttgcttca
      121 tcattcatag aactactata ccatattttg tttcgcagga agtctaatgc taaaatctta
      181 tcagtgagaa gatttcttaa agtttattct ttgtagtttc atctgtatta gtacataatt
      241 taacttagag aaaataggag gactaatcga atggaattaa aagattttac agaaaaagaa
      301 caggaaatga ttaagaaaag gcttacaatg tctaatatta gtgacaaaga aactactgag
      361 aagattctgg cgctagtacc acaagacttg attaagcgaa tcccgttttt tgtcagaaaa
      421 catgctacaa cacgtacgat taaacgcatt tcaattgaac accctgaact ctacgctgta
      481 gctcaaacaa gtggtgagat tccagaaaaa aaacgcgaag aattgcgtca gattatcaca
      541 actatctttg aacaaaagat gaataagcat agtattaagt agagaatgaa aaaatatttt
      601 attggcggtt tgggaagcaa tgcctatcat agcaaggatt ttctttaaga actagattcg
      661 caggtctatt ttctaaatcc atatgaaaag catcttcgag atgaaacaga attgaaatca
      721 tggtttaaaa atgagattgt agaggaagaa tctatctgtc tgataggtca ttctattgga
      781 ggcgatttag ctcgttattt cgcatcggaa tttgaagaag taaagaaact gattcttttg
      841 gatggtggct atctagattt agataagatt ttacctttgg atacagagtt agaggaaact
      901 aaaaattata tcaaatctca aattatttcg gacttagatg ttcttacttc taaagaaaaa
      961 tctgaagcaa agcattggtc agaaaatatg gagaaagctg taagacagtc ctatcactgg
     1021 aatgttgagt ataatagata tgagttggct ataaattatg aaaatataga agcgatactc
     1081 cgcctacgga gaaaaataca agcttttaag agagaagtgg gagatacctt gtttattagt
     1141 cctcgctatc ctaatgaagc tacatggaga gaggaagccc taaaagaatt gccagactat
     1201 tttgatacta tttttttaga aaactttggc catgagcttt atactcaagc acctaaagaa
     1261 atcgctagtc taatgaatga gtggctcgct tattttctat gagctacttg tattgctgga
     1321 aatggtataa gatgaaaatc tacaagtcac taattttcat ataacaaaaa aacgaacagg
     1381 ctctttttct gttattcaga agagaacttg ttcgttttta tcttgatttt cttaaaaagc
     1441 tctatataca tagggatttt ctggtttatc gactttggta aagctgtcga tttccaaggt
     1501 tggaaggttt tgtttgagtt ctttatggta gtgattgacc agttttcctt tggctgtaat
     1561 ccaggtgttg ttttcatact gccgggtatt gcccttggtc agcaatccat agacaccaga
     1621 atctgcgata cagtggataa tgccgaatcg gaacagaaat tgactattgg catgactggg
     1681 gtcgttatag acaaagcctg taaactggat tgtcttgccc tcaaattcat ctggatagtc
     1741 gtagatagcc tccatgactt ccatatagtt ttcattagtg atctgaatac tatcttggga
     1801 taagtattta tccgccgccg ttcgcatttc cttttcatag gctgattttg aaaaataaga
     1861 actagtatct ggtttcaaat attggcttgt agtcccttca cttgtctgaa tagctagatc
     1921 cgttccttcc gataggggga aatgataacc tttagcagaa acggtctgag aatccaagct
     1981 aacagttggg aaagttaagc cgatgacaag tggaatagcc agaagagaaa tactcgtcat
     2041 cttggccaat cggctgttca gatgactgtg ggttttgact tgcttcatcc agatatacaa
     2101 ttgaacaatt gccaagataa aggaaagcac catggagata taggccagat aggaatagtg
     2161 catgttgatg tactggttta gtttgcccga cagatggagg taaatagtca gttcaaaata
     2221 gccagctaaa actaaaaatc gaatcatagg atgactccaa tcaagagaga ataggctaaa
     2281 acgaccagag tcacaatcgt aatgaagtga ctaataaatc gtgctttcaa gtaatttttc
     2341 atcatgagaa tatttttgat atccagcatt gggccaatga cgagaaaggc cagaactggt
     2401 gccaaaccga aactcgagag tagagaagca cctataaagg catccgcctc actacagagc
     2461 gaaagaagaa aggctaaaag catcaagagc aggatagcaa aaagaggagt cgcactgata
     2521 gaggtcagaa tccgagtcgg aacgtagacc tgtattatag aagcaaagag gcagccaaat
     2581 accaaataac gccccgtatc aaaaaattca tcaatggcct gcacaaagac ttgaaaaact
     2641 ttttttgcag aactcaagta agaaaaatca tgctcatgac aagccagacg attttctttc
     2701 tgaatcggtt cttgccagaa aaatcctaga aaaattccta gtattacagc cacaagaatg
     2761 gaacccagag ctcgtaatag tgcgacatgg aaggagttgc caaaggcaga ataggtcgca
     2821 aaaagaacaa tgggattgat aacaggtgct gtcacaagaa aaggaacggc cgtgtaactt
     2881 ggaacctttt tttccagaaa acgattgatg atggggacga ttccacattc acaagaaggg
     2941 aaaagtatac cgacaaaggt cccaaaaaag attctccccc aacgatttcg agggagaaaa
     3001 tgataaacct tgtcaggtgt gatataaact tcaatcagcc ctgagacaat gcttcctatc
     3061 agaacgaagg gaagggcttc aatgataatg gagagaaaaa tggctccagt ttgaagtaca
     3121 ctagatggga gttgttgaaa agcagtcatc tagtttttct tttctgcttt gacttttttc
     3181 tctttgtcat cagggaaagt aacttgtttc aaatcaggga gtttagcaaa ctcttcaaac
     3241 atcttgtcca agtcgtctcg ttttgtaaat ttgacagcca taggtgtacc tcgattctaa
     3301 attctacctc tattatacta ttattttgcg gaaaagggag ttttttaatt tgttttcgat
     3361 agttgataca cttcgaaaat ctcttcaaac cacgtcagct tcgccttacc gtagtacggt
     3421 tactgacttc gtcagttcta tccacaacct caaaacagtg ttttgagctg acttcgtcag
     3481 tcttatccac aacctcaaaa cagtgttttg agctgacttc gtcagtctta tccacaacct
     3541 caaaacagta ttttgagcag cctgcggcta gcttcctagt ttgctttttg attttcattg
     3601 agtatgaaaa ggattggaga aagccttcca gcttgtggta taatagattt atggataaaa
     3661 aatatgaaaa aatctctcag gatttgggag tgacgttaaa gcaaattgat accgttctaa
     3721 gtttgacagc tgaaggggcg actattccct ttatcgcgcg ttatcgcaag gacatgactg
     3781 gtagtctgga tgaggtggcg attaaggcta ttattgattt ggataaaagt ctgacaaatc
     3841 tcaatgaccg taaggaagct gtcttagcta agattcaaga acaaggtaag ttgaccaagg
     3901 aattggaaga agctatctta gttgccgaaa aattagcaga cgttgaagaa ctctatcttc
     3961 cttataagga aaagcgtcgt accaaggcaa ccattgcccg tgaagctgga ctctttcctc
     4021 ttgctcgttt gattttgcag aatatagctg acttagagaa agaagctgaa aagttcgtct
     4081 gtgaaggatt tgcgactggt aaggaagctt tggctggtgc agttgatatc ttggtcgaag
     4141 ccttatcgga agatgtgaca ctgcgtgcta tgacttatca ggaagtgctg agacactcta
     4201 aactcacttc tcaagtcaag gatgaaagtc ttgatgaaaa acaggttttt cagatttatt
     4261 ataatttttc agagacagtt ggaactatgc aaggctatcg taccttggct ctcaatcgtg
     4321 gggagaaact tggtgtcttg aagatcggtt ttgaacatgc gacggaccgt attcttgcct
     4381 tctttgctac tcgtttcaag gtgaaaaatg cttatattga tgaagttgtt cagcaatccg
     4441 ttaagaaaaa ggtcttgcct gctattgagc gtcgtattcg gacagaatta actgagaaag
     4501 ctgaagaggg agctatccaa cttttttctg acaatctgcg caatctcctc ttggttgctc
     4561 cattgaaagg gcgcgtggtt cttgggtttg acccagcctt tcgtacaggt gccaagctag
     4621 ctgtcgtgga tgcaacagga aaaatgctga caactcaggt catctatcct gttaaaccag
     4681 catcagctcg tcaaatcgaa gaagccaaga aagatttagc agatttaatt ggtcaatacg
     4741 gtgtagagat tattgccatt ggaaatggaa cggccagtcg tgaaagtgaa gcttttgtag
     4801 cggaagttct gaaagatttc cctgaagtca gctatgttat cgttaatgaa agtggtgctt
     4861 ctgtctattc tgccagcgaa cttgctcgtc aggagtttcc agacttgacc gttgaaaaac
     4921 gctctgccat ttctatcgcc cgtcgtttgc aagatcctct tgcggaattg gtcaaaatcg
     4981 atcctaagtc aattggtgtc ggtcaatacc aacacgatgt cagtcagaag aaactatctg
     5041 agagtctgga ctttgtcgtg gatacggtgg ttaaccaagt cggtgtcaat atcaatacgg
     5101 ccagtccagc tcttctttct cacgtggctg gactcaataa aaccatttct gaaaatattg
     5161 tcaaataccg tgaggaggaa ggaaaaatca cttcacgctc ccaaatcaag aaagttcctc
     5221 gtttgggggc taaggccttt gaacaggcag ctggtttcct tcgtatccct gaaagtagca
     5281 atatccttga taatacagga gttcacccag aaaattacgc tgccgttaag gaactcttca
     5341 aacgcttgga cattaaggac ctgaatgaag aggcccaaag caaactcaag tcgctttcag
     5401 tcaaggaaat ggcgcaagag ttagaccttg gtccagaaac tcttaaagat atcattgcag
     5461 atcttctcaa accaggtcga gatttccgtg attcctttga tgctccagtt ctccgtcaag
     5521 atgtcctaga tatcaaggac ttagtggttg gacaaaagct agaaggagtg gtgcgtaacg
     5581 ttgttgattt cggtgctttt gttgatatcg gaattcatga ggacggtctc atccatattt
     5641 cccatatgag tcgcaagttt atcaaacacc ctagtcaagt ggtggcagtt ggagatttgg
     5701 tgagcgtttg ggttaagcaa attgatactg aacgtgaaaa agttaatctg tctctcctag
     5761 ctccaaatga aactgactga ttacgtcaag caggtttcac tagaagactt cggcagatct
     5821 tttatccatc atgtccagtg gaataggcgt ctacgttcga caggtgggcg atttttcccc
     5881 aaagatgggc atttggattt taatcccaag gtttatcagg aactggggtt ggacgttttt
     5941 aggaaaattg tccgacatga actctgtcat tatcacctct attttcaagg gaaaggctat
     6001 caacataagg atcgagattt taaggaactg ttgaaagcag tggatggatt acggtttgta
     6061 ccatccttgc ccaatagtaa ctccaagcca atcaagctct atcgttgcca atcctgccag
     6121 caaacttatc agcgtaagcg aagaattgat accagacgct atcgctgtgg actttgtcga
     6181 ggtaaattgc tactaataaa tcagcctgag gactgatgaa agccgagcct acccgtgata
     6241 tactatgtct aacttagcag aaagaggaaa tatcatgaac agtaaatttt ataaaatgag
     6301 acgaaatcgc atgatttcag gagttttggt aggcctgtca gacaagtgga actttgatgt
     6361 tacccttgta cgttttctgt ttgccatttt tacagtcgca aactttggac ttggggtgat
     6421 tatctatatc atcctagctt ctatcatgcc aaccaaggaa gaaatcgaag cagaaatgta
     6481 cggaacaggc ccacgtaaac gcaaagaagc tcaagccatt gacgataatg aaggctggtt
     6541 ttggtgaggt tttacaaaag atttggaata cgcaaaaagc tttaaatcaa tagtctaaac
     6601 tgcttgattt aaagcttttt tacttttata ctcaataaaa atcaaagtgc aaactaggaa
     6661 gccagccgca ggctgtaatt tagtcggcaa tgtgaagtta acatagtttg cactttgatt
     6721 tttgaatagt attaataata tttcgccagc ttcatccaca aactatagtg gattggaact
     6781 agaatagtac acctctactt ctaaaatatt gttagaaatc gatttgacta tcctgatcga
     6841 tttgtcctgt ttttatttca ttttattata aaagcagtaa tttaaactca gttttttaat
     6901 ctatttttgc tgctgagaca atacattttt ggaaaaatgg tggatgctga taacaactga
     6961 taaccagcaa ataacataag cagtgccggt aaattctgta ccgataaagt gtgtgtgttc
     7021 aactaagagc tgaccgatga atggagagag gagatagaga agtccgatga gtatatgttc
     7081 tttgtgtttc attaaagcgc accttctttc cttataggtt gaacgacttg caagtgttaa
     7141 attgaatggt atgttatcta ggtaagctta ctcaaatgat agttcccgct tcctcctttt
     7201 caaagtaagc cttttcacac ttatgtttta caactttaaa aataggaaat caaatcattt
     7261 tctaagtaat tttgcttata tttcacccaa tcttacaaaa atgtaagatt aaagtctctt
     7321 ttgtaaggtt aggttatggc aagcggtctt tttttgatgt aaactagact cataaagaac
     7381 aaaggagtaa catcatgaaa caaatcttac ataagaaaac tagaaaacca agtcacaaag
     7441 aaattgagcg cgttcagctg ggatgcacta tgatgcaagc tacatttcat ttgatgggat
     7501 attaagaaag gagattctca tgacactttt agatgtaaaa cacgttcaaa aaatttataa
     7561 aacacgtttt cagggcaacc aagtagaagc cctcaaggat attcacttta ccgtcgaaaa
     7621 gggtgactac gttgccatca tgggtgagtc tggttctggt aaatcaactc ttctcaatat
     7681 tctagctatg ctggataaac caagtcgtgg tcaggtttac ttgaatggaa ctgacaccgc
     7741 aaccattaaa aattcacagg cctctagctt ccgtcgtgaa aagttgggct ttgtcttcca
     7801 agactttaac ttgctagata ctctatctgt aaaggacaat atcttgcttc cgcttgtctt
     7861 gtcaagaaga cctattacag agatgatgaa gaaattggtg gtgacagctg agaatctggg
     7921 tattaaccaa ttgcaagaga agtaccctta cgagatttct ggtggtcaga aacagcgtgt
     7981 agcagtagcc cgcgccatca tcacagaacc tgaaattctc cttgcggacg agccaacagg
     8041 agcccttgat tccaagtcat ctgcagcctt acttgatgtc tttaatgaaa tcaatgagcg
     8101 tgggcaaacc atcctcatgg taacccactc aacagcagct gctagcaggg ccaagcgtgt
     8161 tctctttatc aaagacggca ttctttacaa ccaaatctac cgtggagaga agacagagcg
     8221 tcagatgttc caagaaatct ctgatacctt gactgtcatg gcaagcgagg tgaattagta
     8281 tgtttcgatt aaccaataag ttagcgatat cgaacctgat taaaaaccgc aaactctact
     8341 atccctttgc actggctgtt ctcttggcag tcaccatcac ctatctcttt tactccctaa
     8401 ccttcaatcc aaagattgcg gaaatccgtg gaggaaccac cattcaagca acacttggat
     8461 ttggtatgtt tgtcgttacc cttgcgtcag ccattatcgt cctctatgcc aatagttttg
     8521 tcatgaaaaa ccgttccaag aaactgggta tatatggcat gttaggcttg gagaagcgcc
     8581 atctaatcag tatgaccttt aaggagttag tgctatttgg gattctaact gttggagcag
     8641 gaatcggtat tggagccttg tttgacaagt tgattttcgc tttcctactc aaactgatga
     8701 aattgaaggt ggaactggtc gctaccttcc agatgaaagt tgtcattaca gtacttcttg
     8761 tcttcggttt gattttccta ggcctcatgt tcctgaatgc ccttcgaatc gcccgtatga
     8821 atgccctcca gctctcacgt gagaaagcta gcggagagaa aagaggtcgt ttcctacctc
     8881 tccaaactat tcttggttcc ataagtttag ggattggcta ttatcttgcc cttacggtaa
     8941 ccgatcctct tacagcccta acaattttct tcctagctgt tttgctggtt atctttggta
     9001 cttatctatt gtttaatgca gggattacag tcttcctaca aatcttaaag aaaaacaaga
     9061 aatactatta ccaacctaat aacctcatat ctgtttccaa cttgattttc cgtatgaaga
     9121 aaaatgcggt tggactagca accatcgcta ttttgtcaac aatggttttg gtaaccatgt
     9181 cagcagcgac aagcattttc aattccgcag aaagctttaa aaaagttcta aatcctcatg
     9241 attttggggt ttcagggcaa aatgttgaaa aagaagattt ggacaaactc ttgagccagt
     9301 ttgcaagtga caaaggttat agtgtcaaag agaaagaagt acttcgttac agtaactttg
     9361 gtattgcaaa tcaagaagga accaagttaa ctatttttga aaaaggacaa aaccgtgtcc
     9421 aacccaaaac agttttcatg gtatttgacc aaaaagatta tgaaaatatg actggtcaaa
     9481 aactgtctct atcaggaaat gaggtcggac tctttgcaaa gaatgaggga gttaaagaac
     9541 agaaagctct aactctaaat gatcatcaat tttctgtcaa agaagaattt actaaagatt
     9601 ttattgtcaa ccatgttcca aatcagttta atattttgac tgctgattac aattaccttg
     9661 ttgtacctga tttacaagcc tttttgaacc aattcccaga ttcggatatc tataatcagt
     9721 tttacggtgg tatgaatgta agtatcagtg aagaagagca actcaaagtc gctgaagaat
     9781 atgaaaaata cttacaaaag tttaatgctc aattaaacac tgaaggtaac tatgtgtatg
     9841 gtagcactct agcagatgct agtgctcaaa tgagtgtcct ctttggcggt gtcttcttta
     9901 ttggtatttt cctatccatt atctttatgg tcggaaccgt tctggtcatc tactacaaac
     9961 aaatttctga aggctatgaa gaccgtgaac gctttattat cttgcagaaa gtcggtttag
    10021 atcaaaagca aatcaagcaa atcatcaaca aacaggtttt aactgttttc ttccttcctt
    10081 tgctttttgc cttcatacat ctagcctttg cctaccatat gcttagcctg attttaaaag
    10141 tgattggtgt actggatacg actatgatgt tgatcgtgac cttgtctatc tgcgctatct
    10201 tcctcatcgc ctatgtgctg attttcatga ttacttcaag aagttatcgc aagattgtgc
    10261 aaatgtaaaa aagatacctc gacttcaaaa tcgaggtatt tcttgtattc taaatgctga
    10321 aaagttgttc gagcaggaag gtaactccca tggtcaagag accaatagct aggttccgaa
    10381 tcatagctgt tctagccgga gcctttccaa gtctggcact agtgtaacca gtgagaagaa
    10441 gggccacacc gacaataagg acagtagcag ggatgcgata ttcacttggg aatatggtca
    10501 ctgacagcat tggaggcaaa cttctaagga aaaaggcaac gaagctagaa atggcagcgt
    10561 gccaaggatt ggtaaattct tcatactcaa tcccatattt ttcctctacc agagccttga
    10621 gtggattttt aagaaagatc ttattagtca agagttgggc agaagttttg cattctccat
    10681 tttggatata agcagcatag agggattttt tggctagttc cctatcttgg tctagcaaga
    10741 gtttttctcg cgaaacggca gcttcctcgg tatcttttgg agttgaaacg gatacatatt
    10801 ctccaccagc cattgaaaag gcaccagcta agatagccgc aaaacctgat aaaaagataa
    10861 tccagatatt ggtcgtggca ctggcaactc cgataaccac accagcaatg gaaataattc
    10921 cattgttagc atcaagaaca cccgcacgca ggatatttaa acgacctgca aaatttgaat
    10981 caatttcgtg atttgtttct gacgctaaat ttcaagttca agttagccat caagaagtct
    11041 tctctgggtg acttgtagtc caagtatttt ttaggatagt tgttgatcca cttttcgatg
    11101 aatgcgactt ctttgggagt cattttcttg gttcccttag gtaaccatct acgaatgagc
    11161 ctgttgcgat tctcattagt tcccctttcc caagaggcat aggggtgcgc ataatagatg
    11221 tgctcctcag aaaatacatc aaacaagcga ttgaattccg ttccattatc tgccgtgatg
    11281 gaaagaatct tgtgttgttt taagatgagt tttagagcct gattgaccac ctcagcactt
    11341 ttatttggaa tcaatcggat gatctgatgt ctactttttc gatccgtcaa gacaagcaag
    11401 cagtagtttt tcgctctcgt aagtagaacc gtatcaatct cataatgccc attctccaag
    11461 cgaaaattga tagcttcagg ccgctgttcg atggattgac cagcaggttt aaagttgatg
    11521 ctagcctgtt tcttatcagg cgaaaatttt tggttatgat agtggagaat cttttccttt
    11581 agttccttgg tcacgcttga tttcttgacc gagcgcttgc aattgttttc ataagactgt
    11641 tgagcatagt cggcagaata aacctctttg aagcgccctt ttccaagaca ttgtcggact
    11701 gtcccacgct tgatttcagt gtggatagtt tgaggaactt ttccaagcag agaggcaatt
    11761 tctctatttg atttcccttc ttttttccat ctttcgatta agcgacggct atcgattgtc
    11821 aaatgtttgg cttttgtagt ataattgtct tgcatctctg tgcctttctt gtgtttgtgg
    11881 ttgaacaaca agtataacac agaggtgttt tcttatgcct acaagagcta tcagctagtt
    11941 gaaccatcta atttttagga ggactgggtg gctaacttca ttatagaact ttcatttacg
    12001 aacatatagt aaaatgaaac aagaacagaa caaatcgatc aggacagtaa aatc
//
LOCUS       DAVLRT010000054        12016 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLRT010000054 DAVLRT010000000
VERSION     DAVLRT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.054"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            368..529
                     /locus_tag="VXM85_001510"
     CDS             368..529
                     /locus_tag="VXM85_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346063.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQDMKNSFD
                     MAKTQIETD"
     gene            621..2300
                     /locus_tag="VXM85_001511"
     CDS             621..2300
                     /locus_tag="VXM85_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1346064.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSDKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIIEELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYKEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            2568..3134
                     /locus_tag="VXM85_001512"
     CDS             2568..3134
                     /locus_tag="VXM85_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346065.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            3456..3644
                     /locus_tag="VXM85_001513"
     CDS             3456..3644
                     /locus_tag="VXM85_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1346066.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3705..4463
                     /locus_tag="VXM85_001514"
     CDS             3705..4463
                     /locus_tag="VXM85_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1346067.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDVRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            4521..5375
                     /locus_tag="VXM85_001515"
     CDS             4521..5375
                     /locus_tag="VXM85_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1346068.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEATYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            5487..6371
                     /locus_tag="VXM85_001516"
     CDS             5487..6371
                     /locus_tag="VXM85_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1346069.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            6534..7307
                     /locus_tag="VXM85_001517"
     CDS             6534..7307
                     /locus_tag="VXM85_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEV1346070.1"
                     /translation="MELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDGVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQ"
     gene            7374..8273
                     /locus_tag="VXM85_001518"
     CDS             7374..8273
                     /locus_tag="VXM85_001518"
                     /inference="COORDINATES: protein motif:HMM:NF012356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1346071.1"
                     /translation="MTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYW
                     IEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAV
                     MNYPLSDSIKDYFLRGIKNTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTA
                     NEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFM
                     KRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAV
                     VLASNCQELENQLVISPDGFVIF"
     gene            8548..9003
                     /locus_tag="VXM85_001519"
     CDS             8548..9003
                     /locus_tag="VXM85_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346072.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            9448..9924
                     /locus_tag="VXM85_001520"
     CDS             9448..9924
                     /locus_tag="VXM85_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1346073.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            9924..10700
                     /locus_tag="VXM85_001521"
     CDS             9924..10700
                     /locus_tag="VXM85_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1346074.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQSIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQENGKDSVEYTKDFAGKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYAINPNPARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAAEVLQEYLFGKWSKVEEEMLKVGEERMRDMSKSNGKDA"
     gene            10684..11493
                     /locus_tag="VXM85_001522"
     CDS             10684..11493
                     /locus_tag="VXM85_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HEV1346075.1"
                     /translation="MEKMHSYIKYLTQEDPIITDEGKQIEILHLDIQDDPEIFEDWAK
                     QFRRNYCSDDELAEMIGFMNISPKEYLENFKLPSDSGIGLSTMSGDFGEILVSDYLQY
                     IEEYVVPRTRYDSKVNKNTSTQGSDVLGYKQDSSNVSNDEVVVIEVKSSASDSSSSKA
                     KNKLQEAINHSDKDFERFSTSIVASYLRLKKSNSDQANVVERFLNITDKPFNVIYGAV
                     AVYSNQSYDIDVIKTVVSKNHRDFQKLRLLVIHSNELMKFIRELYSKASEV"
     gene            11490..>12016
                     /locus_tag="VXM85_001523"
     CDS             11490..>12016
                     /locus_tag="VXM85_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence associated protein"
                     /protein_id="HEV1346076.1"
                     /translation="MIEFGRRSLYFSKLVRSKAKMIEFDIPLESHLPISEDAQKSFLG
                     ALAIVADTARKYFEVYINRKSFNLQLKNQLHNAAEYFDALLVSGLGNSAEYQDYIAIL
                     GTTAYYLCDYNGSSRVMINYISDDIQLLEDCMTLIKVFIDVVTDELFLNHTPIEGKYS
                     SELNTLVESYRNYILS"
BASE COUNT         4259 a         1661 c         2479 g         3617 t
ORIGIN      
        1 ttttaaaaag attaataact ctcgataaat tttgaacctt aacaattgaa tagaccaaga
       61 caagacgtta attatttttt gagcgtaata acctatccgt caagatcttt cggctgaata
      121 attcggcaaa aaaagttctg ctaagcaaaa agaaagtgag cgataagtaa gagtttgaat
      181 atagggaggg ataccctatc cgctatgaag aaaatgagga taatgatact tctaaggttg
      241 aagccggctc atccggaata gaggcggagg tgagattcct atgttgctaa tccaaagcac
      301 ttgtcaatgt caaaaaactt aattaaacaa ataattatta gatataaaac tttagaggag
      361 gtatatattg gacaatgact ggaatggatt ggctgattta atagcaaatc tgattgcaaa
      421 atatgctggt gctttagatt tagataatct acccgatcca acgccagcaa aaaatcaaga
      481 tatgaaaaat agttttgaca tggcaaaaac acaaattgag acggactaat aaaagtgtta
      541 taatatactt gaaatgatag tccaaactta aattaaaaaa attatatatg atatagaaaa
      601 attatattga ggttaatact atgtcaaaag aaaaaataaa agtatacctc tatacacgag
      661 tatctacgtc aatacagata gaggggtatt cgttagaggc acaaaaatca cgaatgaaag
      721 cttttgctat ttacaacgat tatgaaattg ttggagagta tgaagatgcc gggaagtctg
      781 acaagtctat tgaaggtaga atacagttca atcgaatgat ggaagatata aaatctggaa
      841 aagatggagt atcttttgtt cttgtgttta agttatcaag atttgcaaga aatgccgctg
      901 atgttctttc tacacttcag ataatgcagg attatggagt taatcttatc tgcgttgaag
      961 atggcattga ttcatctaaa gatgctggga aactaatgat ttctgtttta tcagctgtgg
     1021 ctgaaataga aagagaaaac atccgtattc aaacaatgga aggtcgcatt caaaaagcaa
     1081 gggaaggaaa atggaatggt ggctttgctc cgtatggata taaacttgaa gacggcaagc
     1141 tgtttataaa tgaggaagag gcagttgcaa taagaacgat tttcgaccag tatgtaaata
     1201 ctacgatagg agccaatggg atatctaaat acttagagaa tcatggaatt agaaaaatcc
     1261 caagacagaa tggtaagaat cctttgtttg atgcaggtct tataagaaag atattaaaga
     1321 atcctgtata taatgggaaa atagcatttg gaagaagaac tttagaaaaa gttcatggta
     1381 caagaaatga atataagcag gttgaacaag atgaatatct aatatctgaa gggatacatg
     1441 aagctatagt ttccgatgaa gtttggcaag ctgctcaggt taagctaaaa tctcaagcaa
     1501 agaaatatga gcatgtgaat aaaggaaaag atacacgcac acacttgctt tctggaattg
     1561 taaaatgccc gatatgtgga gtgggaatgt ttgggaacaa gtgtatcaag aaaaagaaag
     1621 atggcacaaa gtataaagat ttttattact atggttgtaa acataggcag atgataagag
     1681 gtcataagtg tacattcagt aagcaaatta tagaagaatt gttagatgat gctgttgcag
     1741 aggtgattgt caagatagta agtaatccga aatttgcttc tatgatgcaa gaaaaaatca
     1801 acatgaaggt ggatacctct gaaatagaaa aagaaataga taattaccaa aaggaattga
     1861 gaaagagtca ttctacaaag tttaagctaa ttgaggaaat agataattta gatgttgaag
     1921 ataagcatta taagagaagg aaacaggatt tagatgatag actatatcgt atgtatgaca
     1981 aaatagatga attagaatca tcactaattg atgcgaaagc aaagaaacag actattgaag
     2041 ctgaaaagct tacaggagat aacatatata aggttctgat ctattttgat aagctctata
     2101 aagtcatgaa tgatgtagag cgtaggcagt taatttcagc tttgatttct gaaattcaag
     2161 tttataaaga aaaacaatct aacggacaat ggctaaaatc cattactttt aagctaccaa
     2221 ttatagaaga aaatctcaac ataggtttgg acaatgatga acaagttgaa tgtgtaagtt
     2281 tattagagaa acgtagttaa tcctcaaaag gttccccgaa ccttttgagt cccactgacg
     2341 catcacgctg aggcggtatc actgcttgta cgagttgaag catcagcaaa gtaaaagtag
     2401 atgttcagcc aatgcgacga ggtgagaccg aaggtcgata aggtgtgttc tgggtatagg
     2461 actcgtaaag taagtaggaa atatgatgag atgatacaga gagcctgaat tggtcaggtg
     2521 acgtgcttat ttacattagc ctgataatga aaatggagag attatcgatg gttatattgg
     2581 aattgtacca aaatgattat tctaaagatt tagtagcatt tgattccata gaagatggga
     2641 aagcctttgt agctcaaatc ccgggataca ctctggaaac agaagatggt tttgaagtgg
     2701 agtattttaa tcctaaaaac ataccggatt atatggaaat tatttttaac ggaaatattg
     2761 ttcctttatc taaatttatg ttcgatcctg gggaaaatgt aaacattatt tggaaagaga
     2821 tttcaaattt atccctcaaa aatgatagag tgatcgaagg atattcaaaa attgatgcat
     2881 atgtcgttaa caaccatgaa gttaaagcct atgtcgaaac aagagaaacg aactatcgta
     2941 aagcaaaaga cttcttagaa agccgcggat atgaaatcga cagaagcttt ttcggaagtg
     3001 aagacggtga agcgattctt tacagaaaaa aagggatcga agtttggcat ttcttgtgtc
     3061 acttagaccc aatgtttgta gagattgaag atgtagaagg atatgttaaa gaagaagttg
     3121 gagagattca atagtcaacc aaagttcctt gtgagtatat acctttgcta gtaaaagagt
     3181 caaaacgtta taacgaaggg ggggaagaaa atccttgagt gtaagtcttt ttcttgttta
     3241 gagggaaagt attagagtag ccgctcataa tttatactag atttgataac tgatttgaga
     3301 cagattttat ttctataaat attgtgaaaa ttttttaata ccaaagattt aaagttactg
     3361 tgtaaaaaat cctctccact tttaaactaa tgtattataa aagccagttg acacaagtgt
     3421 tacacctgtg atagaattaa cttgaggtga tgaaaatggc aaccgataaa aatagaataa
     3481 tgattagttt ggatgataaa aatctagaga aacttgaaaa tttagtagaa gatgctagag
     3541 atagaagggg aatgcgacta acaaagtccc aggttattga attactccta aataccgttg
     3601 attatttcga tgatattatg ggggctattt actcaaaaaa ataagagcaa aattgctatt
     3661 atttttacaa attggtgtaa cactagtgat ataggagtga tgaaatggtt gtttatatta
     3721 gacaaagtaa attaccaagt gaggtttcaa ttaataaata caatgctcaa gtaggtgcct
     3781 acttacaagg agaagaagtt attttatatc aatctttttc tgaaataaaa gagttaacaa
     3841 gtgaagatat agttgtagat tatataatgg agactagagc attactaaaa atgatgggct
     3901 taaacgttcc ggtttatgac tatcctattg agcttaaaga gttttatggt cgaaagattt
     3961 atgctggtat tttaggagag attgtgaata tacctgataa ttggggaaaa tttattaagc
     4021 ctaaagctgg ttcaaaagtc tttactggaa gagttgttaa tggaacccat gatttaatag
     4081 gtattggtct acctttcgac tatcctatat ggattagtga ggttgtagaa ttcatagctg
     4141 aatggcgttg ttttgtgtta gatgttcgcg tattagatgt tcgaccctat acaggtgatt
     4201 atcatgcaca atttgatgca agtgtaattg atgaagcgat atcatgttgg aaagatgcgc
     4261 caatagctta tggactagat atcggtgtta ctcgcgatgg cagaacactt gttgttgaag
     4321 taaatgatgg ttatgcattg ggaaattatg gcctatctcc tttaaaatca atcaattttc
     4381 atagagctag gtggaaagaa atggtaaaac cctattttga aaaaaatgaa atttttaaaa
     4441 tccaacagga tgttattttc taaaataaat atggtagata accaatataa aaagcatcaa
     4501 aatgaacaag gaggaatctg atgcttggag caattattgg agatattgtc ggttctgttt
     4561 acgaatggaa caatatcaaa acaaaggact ttcctttatt tcggaaggac tgctttttta
     4621 cagatgacac ggttatgacc tgtgctgttg cagaagcaat tatgaatggt ggacaaaaag
     4681 acgatttcat tgacgcgatg aagaaatatg gcagaatgta tccgaatgct gattacggtg
     4741 ctcggtttaa tgcatggcta aacagcgata accgtgaacc ttataatagc tttgggaatg
     4801 gatcagctat gcgtatttct ccatgtgctt ggatcatgga ctgtggtttt tatgcgaaaa
     4861 ctggtatgtg gccatcatct agaggactta cgagtctttc tgcagaggta actcataatc
     4921 atccagaagg tgtcaaaggc gctatggcta cagctgatgc tatctttcta tgtcgttttt
     4981 actttggtgg ttattgtaga gagtacgagc aatcaattaa cgacaatcct acagagtgta
     5041 aaagacgaat taaggattat atagaaaaag aatacgatta caatctatct caaactttag
     5101 atgaaatccg tcctaactat cgttttaacg aaacatgtca ggaaactgtc cctcaagcca
     5161 ttatcgcctt tcttgaaagt agagacttcg aagatgcgat aagaaatgcc atctcacttg
     5221 gtggcgacag tgatactctc gcagcaatca cttgtagcat agcagaggca acttacggta
     5281 ttcctgattg gatcaaggat aaggcctatt cttacttgga tgaaccctta aaggatgtag
     5341 ttaggcgatg ggaaaataga ataaaagcgt attaatctcc agtacgtcag atttctcttt
     5401 tctgtatttt cattgtataa taggattaca tttacttgca tacgacgaaa tgtaaacatt
     5461 tctgtttaaa attaaggata aaatcaatga aaaaagcaat ggtaattatc aaccctactt
     5521 ctggtggcga gaaggctttg gattacaaag aaaagctgga gaataaagca aaagaatact
     5581 ttgaatatgt tgaaaccaaa attaccgaaa aagcgctgga tgcaacacat tttgctgaag
     5641 aagcttctcg tgagcagtat gatgcagtgg ttgtgtttgg tggagatgga actgtcaatg
     5701 aagtcatttc aggtattgat gagagagact acattcctaa gttagggatt atcccaggcg
     5761 gtacgggtaa cctcattaca aaacttttgg aaatcaatca agacatcgat ggcgcaattg
     5821 acgaactgga ttttgattta accaataaga ttgatatcgg taaagcaaat gacaactatt
     5881 ttggttatat ctttagtatc ggttctctgc ctgaggcgat tcacaatgtt gaaatagagg
     5941 acaaaacaaa attcggtatt ttaacctatg ctgtaaatac catgaagtct gtcatgacag
     6001 atcaggtctt taacattaag gttgagacag aaaatggaaa ttatgttggt gaagctagcc
     6061 atgttttggt ccttttgaca aattacttcg ctgataagaa aatctttgaa gaaaacaagg
     6121 acggctatgc caacattttg attctgaaag atgcctctat attctccaaa ttatccgtca
     6181 ttcctgattt attaaaaggg gatgttatcg caaatgataa tatcgagtat atcaaagcgc
     6241 gtaatattaa aatctcttca gatagtgaat tggagtcaga tgttgacgga gataaatcag
     6301 ataacctacc tgtagaaatc aaagtcctag ctcagcgagt agaagtattt tcaaaaccga
     6361 aagaggatta gtatatagag aaagcctttt ttaaggcttt ttgtatactt taaaagatag
     6421 ttcctttaac aacggacatt ccttgcaaat agtttacaaa aatagtatac tggatttatt
     6481 gagtttgaaa acgtttgcgt aaaatttgaa tgaatacttt aggagacaaa ttgatggaat
     6541 taagtgctat ttaccatggg cctgagtcgg agtatgccta tctttataag gataagaaac
     6601 tccatattcg aattcgaact aagaaagggg acattgaaag catcaacttg cactatgggg
     6661 acccttttat ctttatggag gagttttatc aggatacaaa agaaatggtc aagataactt
     6721 ctggtacctt atttgaccat tggcaggttg aagtgtcagt tgactttgca cgtatccagt
     6781 atctctttga gctcagagat acagaaggtc aaaatatttt gtatggcgat aaagggtgtg
     6841 tggaaaattc tctagaaaat cttcatgcaa ttgggaatgg atttaagttg ccttatcttc
     6901 atgagattga tgcctgcaag gttcctgacg gggtttcaaa tacggtatgg tatcagatat
     6961 ttcctgaaag atttgccaat ggcaatgctc tattaaaccc agaagggact ttagactggg
     7021 attcatctgt cacacctaag agtgatgatt tctttggtgg tgatttacag gggattattg
     7081 atcatatgga ttacttgcaa gacttgggta ttactggact atatctttgt cccatctttg
     7141 aatctacaag caatcacaag tacaatacga cagattactt tgaaattgac cgtcattttg
     7201 gagacaagga gacctttcgg gaactggtgg atcaagcgca tcatcgtggc atgaaagtca
     7261 tgctggatgc ggtatttaat catattggtt cgcaatctct tcaatgaaaa aatgtcgtca
     7321 aaaatggtga acagtctgct tataaggatt ggttccatat tcaacaattc ccagtgacaa
     7381 ctgaaaagct agttaataag agagacttac cctatcatgt ttttggtttc gaggactata
     7441 tgcctaagct aaatacagcc aatccagagg tcaagaatta tcttttaaag gttgcgactt
     7501 attggattga agagtttaat atcgatgctt ggcgtttgga tgtggctaat gagattgacc
     7561 atcagttctg gaaggatttt cgtaaggcag ttttagctaa aaatcctgat ctttatatcc
     7621 taggagaagt ctggcataca tctcagcctt ggctaaatgg agatgagttc catgccgtca
     7681 tgaattatcc tttatctgat agtatcaagg actatttctt acgaggaatt aagaatacag
     7741 accagttcat cgatgaaatc aatggagagt ctatgtatta caagcagcag atttcagagg
     7801 tcatgtttaa tctcttggat tcacatgata cagagcgaat cctgtggacg gccaatgaag
     7861 atgttcaact ggttaaatca gccttagcct ttctcttttt acaaaaagga acaccgtgca
     7921 tttattacgg aaccgagcta gccttgactg gaggaccaga tccagattgt cgtcgttgta
     7981 tgccttggga acgtgtatca agtgacaatg atatgctgaa ctttatgaag aggctgatta
     8041 agattcggaa atacgcgtca gtaatcattt cgcatggcaa gtatagcctt caagaaatca
     8101 aatctgatct agtagctctg gaatggaaat acgaaggacg gatcctcaaa gcaatattca
     8161 accaatcaac agaagattat cttttagaga aagaagcagt agtactagca agcaattgcc
     8221 aagaattgga gaatcagctt gtcatctctc cagatggatt tgtgattttc taaaaactag
     8281 ttgatgaaga ttatggtaca tttcatatct tatatagtat aataaggcta gttactaaac
     8341 ttgtaaagga gaacttaaat gaattgtaga ggacatgaaa caagacaaag aattgttaga
     8401 gattttgaag tttagcctaa agcacatatt aagctgttag caaatcaaca aaaacatagt
     8461 gatgcaggag caactattga agatgaatat tatgtattta tcgctgagag taaaattgat
     8521 ggcaagaagg aagttattca gtgttgcatg ggtgcggcaa gggatttttt agaactaatt
     8581 aatcacaaag ggctacctct ttttaatccg cttgtaggtg attctcatgt aaataataga
     8641 caagaatatg acaatacagg gagtggaaat ttacaacctg aaaagtggaa tgaaactgca
     8701 aagcagcttt ataatgttat aatgtggttg attattttat ggaatgctaa gccggataca
     8761 cctttattta attttaaaga cgaagtaatt aagtataaaa catatgagcc ttttgaaagc
     8821 agtataaaaa gagtaaatac tactataaag aatggtagta aagggaaaac tctgactgag
     8881 atgattaatg gctacagagc ggataacgat attagagatg aaatttgtaa ctttaatatt
     8941 ctgaaaaata aaattcgtga tatgaaagac caacaaggaa atacaatgga atcttacttt
     9001 tagttattgt tgaattttgg gtattctata aaatatccta attgagattt aaatagtaga
     9061 ctatacaata tagttaaaat atcagtaaaa acaacacttt attgaggtat tggatacgct
     9121 ttgctaatag cctaataatc acatgtggag tgttgctaca acgaaaaagg tgataatcct
     9181 tgatttcaag ctattttata agcattttgt ctttgtagat aaaggcaatt ttgacaataa
     9241 aaatcctaaa aggtgaatcg ttatagatgt atttgtagat atcgtttgcg catcgaaaaa
     9301 attaatacaa gaataaatat ttatggctct ttaggtgact tttatagaag taaagtttag
     9361 gatagaaaaa cagtcaataa cgcaccattt ttggtgcgtt atgctttttt atgctataat
     9421 ggatttataa aaataaagga gtttgccatg attggaaaaa acataaaatc cttacgtaaa
     9481 acacatgact taacacaaca cgaatttgca cgaattatag gaatttctcg aaatagcttg
     9541 agtcgttatg aaaatggaac tagttcagtc tctacggaat taattgacat catttgtcag
     9601 aagtttaatg tatcttatgt cgatattgta ggagaagata aaatgctcaa tcctgttgaa
     9661 gattatgaat tgactttgaa aattgaaatt gtgaaagaaa gaggtgctaa tctattatct
     9721 cgactctatc gttatcaaga tagtcaggga attagcattg atgatgaatc taatccttgg
     9781 attttaatga gtgatgatct atctgacttg attcagacaa atatctattt agtagaaaat
     9841 tttgatgaaa tagagagata tagcggctat ttggatggaa ttgaacgtat gttagagata
     9901 tctgaaaagc ggatggtagc ttaatggaaa tccaagatta tactgatagt gaattcaaac
     9961 atgctttagc aaggaatctt cgttcactga caagaggaaa aaagtccagt aaacaatcta
    10021 tagcgatttt gcttggaggt caaagtggtg ccggtaagac tacaattcat cgtattaaac
    10081 agaaagaatt tcaaggaaat attgttatca tagatggtga tagttttcgt tctcagcatc
    10141 cacactattt agaactgcag caagaaaatg gcaaagatag cgttgaatac accaaagatt
    10201 ttgcagggaa aatggtagag tctttagtaa ccgaattgag tcatttggga tacaatcttt
    10261 tgatagaggg aactttacga actattgatg ttccaaagaa aacagcacaa ctattaaaaa
    10321 ataaggggta tgaggtacaa ttagccttaa ttgcaaccaa gcccgaattg tcctatctga
    10381 gcacccttat ccgatacgaa gaactgtatg ctatcaatcc aaatccagca cgcgcaactc
    10441 caaaagaaca tcatgatttc attgtaaatc atctagttga taatacacga caattggaag
    10501 aactagctat ttttgaaaga attcaaattt accaacgaga tagaagttgt gtatatgatt
    10561 caaaagaaaa tacaacttca gcagcagaag tgctacaaga atacctcttc ggaaaatgga
    10621 gcaaggtaga ggaagagatg ttgaaggtgg gagaagaacg gatgagggat atgagtaaaa
    10681 gtaatggaaa agatgcatag ttatataaag tatctaactc aagaagatcc tattattact
    10741 gatgaaggta agcagattga gattcttcac ttagatattc aagatgaccc agaaattttt
    10801 gaagattggg caaaacaatt cagaagaaat tattgttctg atgatgaact tgctgaaatg
    10861 ataggtttta tgaatatctc ccctaaagag tatcttgaaa attttaaatt accctcggat
    10921 agtggtattg gcctttcaac gatgtcagga gattttggag aaatattagt atctgattat
    10981 cttcagtata ttgaagaata tgtggttcct aggactcgtt atgatagtaa agtcaataaa
    11041 aatacttcaa ctcaaggaag tgatgttcta ggatataagc aagattcttc gaatgtgtcg
    11101 aatgatgagg ttgtagttat tgaagtaaaa tcatctgcat cagattctag tagctctaaa
    11161 gcgaaaaata agcttcaaga agctattaat cattctgata aagattttga acgattttca
    11221 acctcaattg tagccagcta tttgaggtta aagaaatcta atagtgatca agcaaatgta
    11281 gtagaaagat ttttaaacat aacggataaa ccgtttaatg taatctatgg agctgttgca
    11341 gtctactcca atcaatcata tgatattgat gttatcaaga cagtagtttc aaaaaatcac
    11401 agagattttc agaaattacg actattggtc attcattcta atgaattaat gaaatttatt
    11461 agagaactct attcgaaagc gagtgaggta tgatagaatt tgggagaagg tcattatatt
    11521 tttcaaaatt agtgcgttca aaggcaaaga tgattgaatt tgatattcct ttagaaagtc
    11581 atttaccaat ttcagaggat gctcaaaaat cgtttcttgg ggcactagca attgttgcag
    11641 atactgctag gaagtatttt gaagtttata tcaatcgtaa gagttttaat ttacaattaa
    11701 aaaatcaatt acacaatgct gctgaatatt ttgatgcatt gctggtgtca ggattaggaa
    11761 attcagctga atatcaagac tatattgcaa tattgggtac cacagcttat tatctatgcg
    11821 attataatgg tagttcgaga gttatgatta actatatatc ggatgatatc cagttattag
    11881 aagactgtat gactcttata aaagtattta ttgacgttgt aacggatgaa ctctttttga
    11941 atcatacgcc aatagaaggt aaatactcta gtgagttaaa tactctagtt gaatcatata
    12001 gaaattatat tttatc
//
LOCUS       DAVLRT010000055        11822 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLRT010000055 DAVLRT010000000
VERSION     DAVLRT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.055"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            7..999
                     /locus_tag="VXM85_001524"
     CDS             7..999
                     /locus_tag="VXM85_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1346077.1"
                     /translation="MNGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQKVI
                     EELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNAIVE
                     YFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVY
                     VSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVI
                     YGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFD
                     NIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1604..1681
                     /locus_tag="VXM85_001525"
                     /pseudo
     CDS             1604..1681
                     /locus_tag="VXM85_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2182..3543
                     /gene="trpE"
                     /locus_tag="VXM85_001526"
     CDS             2182..3543
                     /gene="trpE"
                     /locus_tag="VXM85_001526"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1346078.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3540..4106
                     /locus_tag="VXM85_001527"
     CDS             3540..4106
                     /locus_tag="VXM85_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1346079.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4117..5121
                     /gene="trpD"
                     /locus_tag="VXM85_001528"
     CDS             4117..5121
                     /gene="trpD"
                     /locus_tag="VXM85_001528"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1346080.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5118..5885
                     /gene="trpC"
                     /locus_tag="VXM85_001529"
     CDS             5118..5885
                     /gene="trpC"
                     /locus_tag="VXM85_001529"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1346081.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNTGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5872..6471
                     /locus_tag="VXM85_001530"
     CDS             5872..6471
                     /locus_tag="VXM85_001530"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1346082.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6449..7672
                     /gene="trpB"
                     /locus_tag="VXM85_001531"
     CDS             6449..7672
                     /gene="trpB"
                     /locus_tag="VXM85_001531"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1346083.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7665..8441
                     /locus_tag="VXM85_001532"
     CDS             7665..8441
                     /locus_tag="VXM85_001532"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1346084.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8413..8514)
                     /locus_tag="VXM85_001533"
                     /pseudo
     CDS             complement(8413..8514)
                     /locus_tag="VXM85_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            8743..8883
                     /locus_tag="VXM85_001534"
     CDS             8743..8883
                     /locus_tag="VXM85_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346085.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            8920..9474
                     /locus_tag="VXM85_001535"
     CDS             8920..9474
                     /locus_tag="VXM85_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346086.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            9489..10640
                     /locus_tag="VXM85_001536"
     CDS             9489..10640
                     /locus_tag="VXM85_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1346087.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(10692..11351)
                     /locus_tag="VXM85_001537"
     CDS             complement(10692..11351)
                     /locus_tag="VXM85_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1346088.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            11421..>11822
                     /locus_tag="VXM85_001538"
     CDS             11421..>11822
                     /locus_tag="VXM85_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1346089.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQM"
BASE COUNT         3733 a         2078 c         2718 g         3293 t
ORIGIN      
        1 aaaaatataa atgggaaaaa ggtaactatt tatgatattg cacgtttatc aggtttttct
       61 ccaaaaacag tttcacgagt tattaatggt ggagtaaatg ttaaagaaga aacttatcaa
      121 gcgattcaaa aagttataga ggaattgtcg tatattccaa atgcgtatgc aaaaaattta
      181 actaaaaaag aagctataaa tatattgatt tctgtaaaaa aaatcgactc ttttccttta
      241 atttggtttc atacgctgtt agataaagtg ttacggacgt gtaaagaatt tggtgttaat
      301 gctatagttg agtactttgg agaggaggat acaattagta attcaattat atcaagcaca
      361 ggtagcctag tagatggtgt tattgttttt tatgaaagtg tagatgatat cagaattcag
      421 tatttaaaga agaaccatat gccttttctt gttttcggtg aatctcaaac gtctggagta
      481 gtctatgtat ctaataataa ttttcaagct acttatgata tgatgaaagc tgtaactgaa
      541 gaaaaattta aaaatatgtg gttgcttatg ggaggagaat cccatgttaa taaggatcgt
      601 gaaagaggtg tgcgttcttt tctgaatgat aaaaattact ttatggattt aaaagtcatt
      661 tatggcttat ctacaattga ttcagtttat tcttatgcaa tgcagcactt gactacccaa
      721 aattatccgg acattatttt tgtttcagga gatgaaaagg ttcagggctt gattcgtgcg
      781 tgctatgaga aaggaattct tattccagat gatatttcta tcattggatt tgataatatt
      841 cctatttctc agtattatac tccagctttg tctacaattg ctcccaatta tgttaaattg
      901 gctaaagaaa tgatagaagg tgttttagca attattaaag gggaaagtgt cacatctgtt
      961 gaagtttctc ctaaatttgt taggagacag agtttctagt attgtttaat tcttgaaaat
     1021 cctagtggaa aagatggagt gttctaaaat aagttctgtt tagcctccat tcagtttcta
     1081 ttgagttgga tccaatttct aacaagtggg acatgttcat gttcgatcta aggaagatat
     1141 ggtcacattc gaatgatatc aaaaaaatcg taaaaaagtc ttgacaaaga aaaaacaaag
     1201 tattaaaata agttcaacaa atcagaaaag taactatttt tgatcttcag ggagcctgtg
     1261 gtgattgtga ataggtggtt ggaagtagtg aaagtgggct gattttgaaa atgaatttga
     1321 aacaatgaaa attcggtgcg cacaccttac agtgcagctt gttgttaaac aaggcagaga
     1381 tgtaaaggga tagtcccttt ataattgagg tggcaccgcg ttaccaacgc cctcacacgg
     1441 aagtatattc tgtgtgtggg cttttttcta tccgtcgttt ggtttatctt ttattagggc
     1501 cttaagtcgc tttgatgaac ttgagttcta tctacagctc cttgcctcct actaaaagta
     1561 aacaaaaagg ctggactaat acgaaaaaag ctagtaatac tcaatgaaaa tcaaagagca
     1621 aactaggaaa ctagccgcag gttgctcaaa acactgtttt gaggttgtgg atagaactga
     1681 cgaagtcagc tcaaaacact gttttgaggt tgcagataga actgacgaag tcagtaacca
     1741 tacctacggc aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt ataaaggtgt
     1801 accgagatga acatctcttc atcaatgtct ctggtcgtgg aggcaaggac ctagctgcga
     1861 ttgcagatta ctagaagcta aaaaataata gatggaaaaa ttacagtctt ttctctcaca
     1921 gagagcttgt ggttgctgaa aacaagcaga gaaagctgta aagttgggtt catctgaaac
     1981 gagagaagaa aacatcattc tcaagggagt gccctttatc gcacagcctg ttacaggaga
     2041 tatctgagac aggaatgaga gataggagaa atcctataat tgaggtggca ccgcgaattt
     2101 cgtcctcacg caagttattt tgcgtgggga tttttcatac agtcgccacg aactagaatt
     2161 agaactgaaa gggaaatgac aatggaacga atcattcatg gagatgtctt atcaccaatc
     2221 ttggcttata tgcgcttaaa ggggcaacat aaggttattt tagagagtat tccgagagac
     2281 aaggaaacag ctcgtttttc tatcctagcc tataatccag tttttgagat taagtttgaa
     2341 aatggagttc tctatcaaaa tggtcaagcg attgatcggg atcccttgga tttcctttat
     2401 gaagtgactc ataagagcca gcaccattca gacctccctt ttggtggggg agccattggt
     2461 tttgttggtt acgatatgat ttcgctttat gaagaaattg gtcaaatccc tgaggataca
     2521 attgggacgc cagacatgca tttctttgtc tatgagagct atatggtctt tgaccacaag
     2581 aaggagaaga tacatgtcat agaggatgca ctttatagtg agcgcagtca agaggccttg
     2641 gaaaaatcct tgaaccaagt gcttgaggaa ttacgcattc ctgctccaaa tgaatttgaa
     2701 gacttggatt tatcaccact tgacttcaaa ccgcatatcg ctccacataa gtttgaggga
     2761 atggtagaaa cagctcgtga cttgattcgt aacggagata tgttccaatg tgtactcagc
     2821 cagcgtttct cagcagaagt tactggaaat ccatttgact tctacagaaa tctccgcgtg
     2881 actaatccat ctaattacct ttatttctat gattttgggg attatcaaat catcggagct
     2941 agtccagaaa gtttggtttc tgtcaaaaat ggcatcgtga caactaatcc gattgcaggg
     3001 acgcgaccaa gaggggctac ggatgaagaa gacaaggcct tggcgactga cctcctctct
     3061 gatgagaagg aaacagcaga acatcgaatg ttggtagact tggggcgtaa cgatattggc
     3121 cgcatctctg aaacggccag tgtccaagtt actaagtata tggaggtgga gctcttccgc
     3181 tacgtcatgc atttgaccag tgtggtcaag ggacgtttgc ttgcagaact cactgccatg
     3241 gatgccttga aagcaacgct tcctgctgga accgtttctg gagcaccaaa aattcgagct
     3301 atgagacgca tctatgaact ggaaacggaa aaacggagcg tatatgcagg agcaatcggc
     3361 tacttgtctg cgacgggtga tatggatttg gccattgcca tccgaaccat gattctcaaa
     3421 aatcaaagag cctatgtgca ggctggggca gggattgtct acgactctat cgcccaaaac
     3481 gaataccaag aaaccattaa caaagcaaaa tctatgacta gaattggaga actaagacca
     3541 tgattttatt gattgacaac tatgattctt ttacctataa cttggcccaa tacattggga
     3601 attttgcaga agttcaggtt ttgagaaatg atgattccaa gctgtatgaa gaagctgaaa
     3661 aagcagatgg tctggtcttt tctcctggtc ctggttggcc agttgatgct ggaaagatgg
     3721 aagacatgat ttgtgatttt gcaggcaaga agccgattct agggatttgt ttgggtcacc
     3781 aagccattgc agaagtcttt ggtggtaagc taggtttggc tccaaaagtc atgcatggga
     3841 aacagagcaa tatcaacttt gaagcgccat ctgttttgta tcaaggtatt gaggatggac
     3901 gtgcggtcat gcgttatcac agtattttga ttgaggaaat gccagaagac tttgaagtga
     3961 cagctcgttc gactgatgac caagccatca tggggattca acataaaaac ctaccgattt
     4021 atggcttcca gtaccatcca gagagcattg gaacgccaga cggcttgtct tctattcgga
     4081 attttatcga gaaggttgta aagtgaggaa actaggatga aagagattat tgaaaaacta
     4141 gcaaaatttg aaaatttatc aggtgtggaa atgacggatg tcattgagcg tatcgtaact
     4201 gggcgtgtaa ctgaagcgca gattgcttct ctcctcttag ctcttaagat gaagggggaa
     4261 acacctgaag aacgtacagc cattgcccaa gtcatgagag gacatgccca gcatattcca
     4321 actgagattc atgatgctat ggacaactgt ggtacaggtg gggacaagtc tttcagtttt
     4381 aatatttcca caactgcagc ctttgtcttg gctggtggcg gtattcacat ggccaagcat
     4441 ggtaaccgct cgatttcttc taaatctggt tccgcagatg tcctcgaagc cttgggaatc
     4501 aatcttgacc tcaaaccagc tgaactaggt aaggtctttg ataaaactgg aatcgtcttt
     4561 ctcttcgcta aaaatatgca cccagctatg aaatacatca tgccagctcg tttggaattg
     4621 ggaattccaa cgattatgaa cttgactggt ccactgattc atccaatggc tttagaaaca
     4681 cagcttcttg gaattagtcg tccagaactc ctagaaagta cagctcaggt tttgaaaaat
     4741 atgggtcgca aacgtgccat cgtggttgct ggaccagaag ggttggatga agctggcttg
     4801 aacggaacaa ccaagattgc acttcttgaa aatggcgaaa tcagcttgtc aagctttact
     4861 ccagaggatt tgggaatgga agactatgct atggaagata ttcgtggtgg gaatgctcag
     4921 gaaaatgcag aaattttgct tagcgttctg aaaaacgaag caagtccatt cttggaaacg
     4981 acagtcttga atgctggtct tggtttctat gctaatggta agattgatag catcaaggaa
     5041 ggagttgcct tggcccgtca agtgattgct agaggcaagg cccttgaaaa actcagactg
     5101 ttacaggagt accaaaaatg agtcaggaat ttttagcacg aatcttagag cagaaggcgc
     5161 gtgaggtgga gcagatgaag ctggagcaaa tccagcctct gcgccagacc tatcgcttgg
     5221 cagaattttt gaagaatcat caggactgct tgcaggtaat cgctgaggtc aagaaagcta
     5281 gccctagttt gggagatatc aatctcgatg tggatattgt gcaacaggcc cagacttatg
     5341 aagaaaacgg agcagtgatg atttcggtgt tgacagatga ggttttcttt aaagggcatt
     5401 tggattatct acgggaaatt tccagtcagg tagagattcc gacgctcaac aaagacttta
     5461 tcatagatga aaagcaaatc atccgcgctc gcaatacagg tgcgacagtt atcttgctta
     5521 ttgtggcagc cttgtccgaa gaacgcctca aggaactgta tgactacgcg acagagcttg
     5581 gtctggaagt cttagtggag actcacaatc tagctgaact agaggtagcc cacagacttg
     5641 gtgctgagat tatcggggtc aacaaccgca acttgactac ctttgaggtc gacttgcaga
     5701 ccagtgtaga tttagcccct tactttgagg aaggtcgcta ttacatttct gaatctgcca
     5761 ttttcacagg gcaggatgcg gaacgactag ccccatactt taacggaatt ttggtaggga
     5821 cagctcttat gcaggcagaa aatgtggccc agagaatcaa ggagttgcag attgacaaag
     5881 gttaaaattt gtggactatc gaccaaagaa gcggtggaaa cagccgtttc agcaggagcc
     5941 gactatatcg gttttgtctt tgcacctagt aaaagacagg tgactttaga agaggcagct
     6001 gagttggcaa agcttattcc tgcagatgtg aaaaaggttg gagtatttgt ttcaccaagt
     6061 cgggtagaac tgctggaagc gattgacaaa gttggcttgg acttggttca agttcacggt
     6121 caggtggcag ctgatttatt tgagaatttg ccttgtgcca gtattcaggc tgtgcaggta
     6181 gatggaaatg ggcatgtccc caattctcag gcagattatc tactctttga tgcccctgtg
     6241 gcaggaagtg gtcagtcctt tgattggggt caactggata cgactggact agcacagccc
     6301 ttctttatcg caggtggcct taatgaagat aatgtagtaa aagcaattca acattttact
     6361 ccctatgcag tagatgtatc gagcggagtg gagacagatg gacaaaaaga tcatgaaaag
     6421 attagaagat ttatagagag ggtaaagcat ggcatatcag gaaccaaata aagatggatt
     6481 ttacggaaaa ttcggcggac gttttgtccc agaaacattg atgacagcag ttttggagtt
     6541 ggagaaggcc taccgtgaaa gtcaggcaga cccaagtttc caagaggaat taaaccagct
     6601 tttgcgccag tacgtaggac gtgaaactcc tctttattac gcaaaaaact tgacccagca
     6661 tatcggcgga gccaagattt atctcaaacg ggaagacctt aaccatacag gagcccacaa
     6721 gattaacaat gccttgggac aagtttggct tgccaaacgc atgggtaaaa agaaaattat
     6781 cgcagaaact ggtgctggtc agcacggtgt ggcaactgca actgctgcgg ccctctttaa
     6841 catggaatgt accatctaca tgggtgagga agatgtcaaa cgccaagccc tcaatgtctt
     6901 ccgtatggag cttttgggag ctaaggtcga ggccgtgaca gatggttcgc gcgtgctcaa
     6961 ggatgcggtc aatgcagctc ttcgttcatg ggtggctaat atagatgata cccactatat
     7021 ccttggttct gccttggggc ctcatccatt tccagaaatt gttcgtgact tccaaagtgt
     7081 cattggtcga gaggctaaac aacagtaccg tgacttgaca ggacaaaatc tgccagatgc
     7141 cctagtagcc tgtgttggtg gtgggtcgaa tgccattggt ctcttccatc cctttgtaga
     7201 agatgagtca gtagccatgt atggagctga agcggctgga cttggtgtgg atacggagca
     7261 ccacgcagct accttgacca agggtcgtcc aggtgtcctt cacggttccc tcatggatgt
     7321 gctccaagat gcccatggtc aaattcttga agccttctct atctcagcag gtttggacta
     7381 tcctggtatc ggtccagagc attctcacta ccacgatatt aaacgtgcca gctatgtccc
     7441 tgtgacagac gaagaagcct tggaaggatt ccaactcttg tctcgtgtgg aagggattat
     7501 cccagccttg gaatctagcc atgctatcgc ctttgcggtg aaattggcca aagaacttgg
     7561 accagaaaag tctatgattg tctgcctatc aggtcgtggg gacaaggatg tggttcaagt
     7621 caaagaccgc ttggaagcag atgcagcaaa gaagggagaa gctcatgcct aagacactaa
     7681 caaaaaaatt aaatgctatt aaagcagctg gaaaaggaat ttttgttccc tatatcatgg
     7741 ctggtgacca cgaaaaaggt ttggatggtc ttgctgaaac aattcacttt ttagaagatt
     7801 tgggtgtctc tgcaattgaa gtgggtattc ccttttcaga ccctgttgca gatggacctg
     7861 ttatcgaaga agctggcttg cgcagtttag cccacgggac ctctacccag gctttagttg
     7921 aaaccttgaa aaccattgaa acagagattc cactggtcat catgacctac ttcaaccccc
     7981 tctttcagta cggtgtggag aactttgtca aagatctggc tgatacagct gtgaagggct
     8041 tgattatccc agacctgcct catgaacatg ccaactttgt agaaccattt ttggcagata
     8101 cagatatcgc cttgattcct ttagtaagct tgaccacagg aattgagcgc cagaaagagt
     8161 tgattgaagg ggcggaggga ttcgtctatg ccgttgccat caatggggta acagggaaat
     8221 caggcaatta ccgtgcagat ttggacaagc acttggcgca acttcatcaa gtggccgaca
     8281 tcccagtctt gacaggtttt ggtgtatcta gtcaagccga tctggaacgc ttcaatgcgg
     8341 tgtcagatgg tgttatcgtc ggttcgaaaa tagtaaaagc tctccaccaa ggagagccga
     8401 ttcaggactt tatcaaacaa gcagtagctt accaaaaata attaaacaag tagcaagtaa
     8461 gaggaaaggc acgaaaggaa gtctttcctt tttcttttgc aggcgaaagg ctagttttat
     8521 agtgaattga atctggaata ttacactacg gttcctaaaa tattttaaga aagtgatttg
     8581 aatttcccaa taaatttgtt cagtttttaa ttgattttac tatagaagat ataattttct
     8641 aaattactct gacacagaac tttaagaaaa agctaattat tgtagtaatt tctttaagaa
     8701 aatgatataa ttcattaaaa agtatttgga ggaggaagaa ttatgttgaa tcaggatctc
     8761 tttgattcgc ttgaggcaca aaaaattgta gatactttga tgaaagggca aaaagattat
     8821 gtagatgaac gtctagaaaa aagagagaca atgatagtgt cgaatggtta tgcatggacg
     8881 tgacctaatc atattgatac tgcatttgca tcagcagatt tgtttgagta taaattacaa
     8941 ttagcaggac agacttgggg atatttagaa tttgaaacaa atacagaaaa atatgggaaa
     9001 gtattgttaa ttataaaggg taagaagcga cttacgaacc aatttccttt ggtacaaaaa
     9061 aataagagtg gctacttatt tgaatatgct cagatgaata cactttatct taatcaacat
     9121 tcttcctaca aaaatgatga agatagtcat tcctttccaa ttcagatgga gttagtttct
     9181 gatgaaatga ttcaagaaat tgaacaagct actaaaaatt cgaatatcga aaaatttatg
     9241 attttaactt atgaggcgga ctcagaaaac aatattatat ctgtagatgt tgttatgcct
     9301 gatgcacgaa ctggtcagtt acacttgatt caagatttgt ctgagtatat tcaatcaagt
     9361 tcgtaccatt tcgaagaagc taaatatcaa gatattccta atttttcaga attatcggaa
     9421 acagaagatt ttgaaattat tccaagaata gaaaaacaag aaggtcaaaa gtagtaagga
     9481 atatgtaaat gtttaatggt cgggtattga aagaattacg gctgttaaat ggtttaagta
     9541 gagcagaatt agctcagaga attaatttaa cggaacaagc catttggcag tttgagtcca
     9601 acgaaacgaa acctaaatta tcaaccaaaa tgcatttggc caaccaattt catgttgatt
     9661 taacttattt tgaacaggaa gaagagagca ttcgatttga ttcttctgta attgccttta
     9721 gaaatgcaga cctagcaaca cggaaaacaa tagatattca aactatgtat ttacataagg
     9781 tagatagttt gattgattat tttgaaagtt ttgtaattat acctaatatt acaattcatg
     9841 acctaagtaa tgtagtgagt gaatcttatc ataagggaga atccattgag gaattggctc
     9901 tttatgccag ggaaaaatta ggtatttcaa aagataatca tgatttgctt tataaattag
     9961 aacgttcagg catctatatc gtggaacgat taattaatgg ccaagctgat gcttatagcg
    10021 catggtcaaa attgggaaga ccttatattg tgttgggaac gaataaatca tctgtacgtc
    10081 gaaattttga cttagctcat gagctaggac atattctttt acacaaatat aaagatatga
    10141 atgaagatgg caatcgtttg gagcaagaag caaattattt tgcatcatgt tttttattgc
    10201 caaaagaaga gtttttagtc aaatttgaag agagggttgg caagcgtgtc agcaatcctg
    10261 atagttatat tttattgaag tcggatttga atgtttcgat acaggcttta gagtatcgag
    10321 cttttaagtt aggattattg actccaaagc aacattctta cttttatcgt caaattgcgc
    10381 aaaaaggtta caaaatgatt gaacctttgg atgatcaaat ttttgttaaa aaaccaagca
    10441 aagtaaagag tattctggac gtcgttttga gtaatcatct agtcagtcta gcgactataa
    10501 tgtctaaaca aagtattcgt ttacagttta taagcgaaat attttcagtc gaaatgaaat
    10561 tttttgatca gtatcaagaa gatagaagaa cagatcgatt tgataacatc atccctttgt
    10621 acaaaagaaa taatttataa tttaactgtg aaataatttc atgaaggaca tatggcagaa
    10681 attggatttt atcagacaag cagtagctta ccaaaaataa tcacacaagc agcaagtaag
    10741 aggaaaggca cgaaaggaag tctttccttt ttcttttgca ggagaaaggc cagaataccc
    10801 gtcgcagaag cgaactgaat caagatcagt aactccgtta cgctaaagac gagagcacaa
    10861 gaagctaaaa agaggaaatc tcctgcgccc atgcggatat cgataaaatg agccaaaatt
    10921 ccaagagcaa ggaaggagac catgaccaga ttccagccag aggaagctat taggattagg
    10981 tggaaagtca tccagaccag taagggatat tcctgatggc gaaagtcgta gatgcctaag
    11041 gtcaaaccag cggtgattag gacgacttgc cccaaggaaa gcaattccca agagtaaagc
    11101 agaaagagga gtcctaagac taattcaaag agggcatacc agacaggata gcgaactttg
    11161 cagtagcgac agcgaaagcg attgaagacc tgtgagagaa tcggaatcaa atctaaggga
    11221 cgcaagcgag tctgacagga atcgcagtga ctggctgaac tgataatgga ttgctctgga
    11281 aaacggtcaa tgaccaaacc aagaaaggaa gcgagaatgc tcccgacgag aaaaaaataa
    11341 aaatcaatca tacttatcta ttcgtaaaaa atgggagaaa tagtataatg gagaaacata
    11401 gataagatgg tgaggaaaag atgacaattc gttttgaaga aaaggtgagt atagaaaacg
    11461 ctcagctcgt atgccaatgg tccaactcct taggcaaagt ctttcaagaa caatggatgg
    11521 gaccaaggat tccttttcta ctgacccttc aagccttgga aggagtcttt tcaatctttg
    11581 atgaacaaga gtttgtggga cttatccaga aaatcaggct agaagacagc aatcttcata
    11641 tcgggagatt ttttatcaac ccccagaaac aggagcaagg cttaggtagc caggctttaa
    11701 ggaaatttgt tagtttggcc tttgaaaatg aagatataga tagtatttct ctaaatgtct
    11761 tcgaggcaaa tcaaagagct cagaatcttt accaaaaaga aggatttgaa atcgttcaaa
    11821 tg
//
LOCUS       DAVLRT010000056        11820 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLRT010000056 DAVLRT010000000
VERSION     DAVLRT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11820
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.056"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(128..1759)
                     /locus_tag="VXM85_001539"
     CDS             complement(128..1759)
                     /locus_tag="VXM85_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1346090.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2052..6962
                     /locus_tag="VXM85_001540"
     CDS             2052..6962
                     /locus_tag="VXM85_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1346091.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTPAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGKWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEPKKDYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHETSTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGSALAPLLEKNK
                     ALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNKVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVFEVYENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            7052..8248
                     /locus_tag="VXM85_001541"
     CDS             7052..8248
                     /locus_tag="VXM85_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1346092.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8384..8911
                     /locus_tag="VXM85_001542"
     CDS             8384..8911
                     /locus_tag="VXM85_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1346093.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            8988..9344
                     /gene="glnR"
                     /locus_tag="VXM85_001543"
     CDS             8988..9344
                     /gene="glnR"
                     /locus_tag="VXM85_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1346094.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9381..10727
                     /gene="glnA"
                     /locus_tag="VXM85_001544"
     CDS             9381..10727
                     /gene="glnA"
                     /locus_tag="VXM85_001544"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1346095.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10823..10942)
                     /locus_tag="VXM85_001545"
     CDS             complement(10823..10942)
                     /locus_tag="VXM85_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1346096.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11430..11820
                     /locus_tag="VXM85_001546"
                     /pseudo
     CDS             11430..11820
                     /locus_tag="VXM85_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3784 a         2157 c         2553 g         3326 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatatac tatatatctg cagaatactc tttcagtttc
       61 attcagccta gtcttgctta ggaaaaagta aaaagccatc caattatgat ggatagcttc
      121 ttggttctta gatttgttct gcaaaaacct tttcagcaag gttcatagcg tggtctgata
      181 cacgagtgta gtgtgagatg atgtcgataa agttgacccc agcttgtgtt gaacattcgc
      241 ctttgttgag gcgtttgatg tgggtttttc tgagaacacg ttctatctta ttgattgctt
      301 catgacgttc tacaagactg cgtgcttttt caatatcatt gttttccaca ctatccagag
      361 catctttgat aaagtcacta gtttggcggt aaacttcctc taattctttc aaggcggcat
      421 cagaaaattc aacatttttc cgttgaagat agtcagtcag attgagtaga gcctccgtgt
      481 ggtctccaat ccgttccaaa tcacgggagg aatcaaggat attggtaagc acttcacttt
      541 ctttttggct gagagcttcg cttgaaaggg caatgagata acgtgttaat tgctcatcga
      601 tggtgttaat tgcttcttcg gttttatgcc ctttttcagc aactttttca tccaagtcaa
      661 tgatgtactt ataggaaagg tcaaaggctt tagcagcgta gtttcctaag tgcaagagct
      721 ctttcttagc atttcctaga gcgatagatg gggcctgttt gatgaaatgt tcatcaagat
      781 ataagggttc gtatttgaca acctcgtcct ctccaggaat aatcttggtt acaaagtaag
      841 ccagagctcc gataaatgga aattggacaa tggtgttggt aatattaaag gttccgtgag
      901 caaaggcgat ggtcatttcc ggtgctagat ttagcgtagc ttcaaaccaa tgaatcagga
      961 cagtaaaagg aactagaaaa ataacgcaga cgactgttcc gataacgttg aaggcaacat
     1021 gagctcctgc tacccgttta gctgcaatat tagcccctaa agaggcaatg atggctgtaa
     1081 tggttgtccc gatattgtca ccaaatagaa ctggcaaagc tccctgtagg tcaattagat
     1141 tgccggcgta gaggttttgt aaaatcccaa tggtagccga agaagcttga attagcaagg
     1201 tcaagccggt accgacaaag acacccaaaa caggattctt acttagctca atcatatagt
     1261 ccttaaagac ctgtaaatcc ttgagtggag ccattgcgcc gctcatgaga ttgagggcaa
     1321 aaaagatacc accgacacca aagaggatgc gtccgatatt attgactgtc cgattttttg
     1381 taaaaaagag acagacggca ccgataaaga gcataggtag ggcatagtta cctaatttaa
     1441 aaccgatgag aaaggatgtg acagttgtcc caatattagc acccatgaca atcccgatag
     1501 cctgacgtaa ggttaagaga ccggcactga ccaggccgac tgtaataact gttacaccag
     1561 aactagactg aattagagca gtcatcccaa taccaaccag aactccaaag aaaggattac
     1621 tagtatattt gtcaatgtaa aaacgaaggc gatctccagc agcttgttgc aaaccgtctc
     1681 ccatggtctt gatgctatat aagaatagcc ccagaccacc taaaaagtga aataaaattt
     1741 cctgccaatt aatggacatt tctttttcct ccgaaaaata atcgcggaat atctcctatt
     1801 ctattttaaa ggataaaagt aaatctaaca agtgttaatg tcaagtttgt aaaagaaaat
     1861 tataattgat aagtgtatta tttttaagag tttttaacct tattttagat ttgtgcaaac
     1921 agttattgtt ttttgtctaa aaatgtttta ggataaaaat gtgattatca tggttctaga
     1981 gtttgataaa aaactacaga atataaagcg ctttctttaa aacaacaaaa tcaatctttt
     2041 aggaggagaa aatgaagaat ccattttttg aaagacgttg tcgttacagt attcgtaagt
     2101 tatcagtagg agcctgctcg ctgatgattg gtgctgtttt atttgctggt ccagccttgg
     2161 ctgaagaaac tgcagttcct gaaaatagcg gagctaatac agagcttgtt tcaggagaga
     2221 gtgagcattc gaccaatgaa gctgataagc agaatgaagg ggaacatgct agagaaaaca
     2281 agctagaaaa ggcagaagca gctagcgcag ctaaaccaga ggaaaaagca ggtgaggtgg
     2341 ttgcagaaac accatctgca gaagcaaaac ctaagtctga caaggaaaca gaagcaaagc
     2401 ccgaagcaac taaccaaggg gatgagtcta agccagcagc agaagctaat aagactgaaa
     2461 aagaagtcca gccagatgtc cctaaaaata cagaaaaaac attaaaacca aaggaaatca
     2521 aatttaattc ttgggaagaa ttgttaaaat gggaaccagg tgctcgtgaa gatgatgcta
     2581 ttaaccgcgg atctgttgtc ctcgcttcac gtcggacagg tcatttagtc aatgaaaaag
     2641 ctagcaagga agcaaaagtt caagccttat caaacaccaa ttctaaagca aaagaccatg
     2701 cttctgttgg tggagaagag ttcaaggcct atgcttttga ctattggcaa tatctagatt
     2761 caatggtctt ctgggaaggt ctcgtaccaa ctcctgacgt tattgatgca ggtcaccgta
     2821 acggggttcc tgtatacggt acactcttct tcaactggtc taatagtatt gcagatcaag
     2881 aaagatttgc tgaagctttg aagcaagacg cagatggtag cttcccaatt gcccgtaaat
     2941 tggtagacat ggccaagtat tatggctatg atggctattt catcaaccaa gaaacaactg
     3001 gagatttggt taaacctctt ggagaaaaga tgcgccagtt tatgctctat agcaaggaat
     3061 atgctgctaa ggtaaaccat ccaatcaagt attcttggta tgatgccatg acctataact
     3121 atggacgtta ccatcaagat ggtttgggag aatacaacta ccaattcatg caaccagaag
     3181 gagataaggt tccggcagat aacttctttg ctaactttaa ctgggataag gctaaaaatg
     3241 attacactat tgcaactgcc aactggattg gtcgtaatcc ttatgatgta tttgcaggtt
     3301 tggaattgca acagggtggt tcctacaaga caaaggttaa gtggaatgac attttagacg
     3361 aaaatgggaa attgcgcctt tctcttggtt tatttgcccc agataccatt acaagtttag
     3421 gaaaaactgg tgaagattat cataaaaatg aagatatctt ctttacaggt tatcaaggag
     3481 accctactgg tcaaaaacca ggtgacaaag attggtatgg tattgctaac ctagttgcgg
     3541 accgtacgcc agcggtaggt aatactttta ctacttcttt taatacaggt catggtaaaa
     3601 aatggttcgt agatggtaag gtttctaagg attctgagtg gaattatcgt tcagtatcag
     3661 gtgttcttcc aacatggcgc tggtggcaga cttcaacagg ggaaaaactt cgtgcagaat
     3721 atgattttac agatgcctat aatggcggaa attcccttaa attctctggt gatgtagccg
     3781 gtaagacaga tcaggatgtg agactttatt ctactaagtt agaagtaact gagaagacca
     3841 aacttcgtgt tgcccacaag ggaggaaaag gttctaaagt ttatatggca ttctctacaa
     3901 ctccagacta caaattcgat gatgcagatg catggaaaga gctaaccctt tctgacaact
     3961 ggacaaatga agaatttgat cttagctcac tagcgggtaa aaccatctat gcagtcaaac
     4021 tatttttcga gcatgaaggt gctgtaaaag attatcagtt taacctagga caattaacta
     4081 tctcggacaa tcaccaagag ccacaatcgc cgacaagctt ttctgtagtg aaacaatctc
     4141 ttaaaaatgc ccaagaagcg gaagcagttg tgcaatttaa aggcaacaag gatgcagatt
     4201 tctatgaagt ttatgaaaaa gatggagaca gctggaaatt actaactggc tcatcttcta
     4261 caactattta tctaccaaaa gttagccgct cagcaagtgc tcagggtaca actcaagaac
     4321 tgaaggttgt agcagtcggt aaaaatggag ttcgttcaga agctgcaacc acaacctttg
     4381 agtgggggat gactgtccaa gacacaagtc ttccaagacc tctagcagaa aacatcgttc
     4441 caggggctac tgttatcgga agtaccttcc cagatacaga aggtggagaa ggtattgaag
     4501 gtatgttgaa cggtaccatt actagcttgt cagataaatg gtcttcagct cagttgagtg
     4561 gtagtgtgga tattcgtttg accaagccac gtaccgttgt tagatgggtc atggatcatg
     4621 caggggctgg tggtgagtct gttaacgatg gcttgatgaa caccaaagac tttgaccttt
     4681 attataaaga tgcagatggt aagtggaagc tagctaagga agtccgtggc aacaaagcac
     4741 acgtgacaga tatcactctt gataaaccaa tcactgctca agactggcgc ttgaatgttg
     4801 tcacttctga caatggaact ccatggaagg ctattcgtat ctataactgg aaaatgtatg
     4861 aaaagcttga tactgagagt gtcaatattc cgatggccaa ggctgcagcc cgttctctag
     4921 gcaataacaa ggtacaagtt ggctttgcag atgtaccggc tggagcaact attaccgttt
     4981 atgataatcc aaattctcaa actccactcg caaccttgaa gagcgaagtt ggaggagacc
     5041 tagcaagtgc accattggat ttgacaaatc aatctggtct tctttattat cgtacccagt
     5101 tgccaggcaa ggaaattagt aatgtcctag cagtttccgt tccaaaagat gacagaagaa
     5161 tcaagtcagt cagcctagaa acaggaccta agaaaacaag ctacgccgaa ggggaggatt
     5221 tggaccttag aggtggtgtt cttcgagttc agtatgaagg aggaactgag gacgaactca
     5281 ttcgcctaac tcacgcaggt gtatcagtat caggttttga tacgcatcat aagggagaac
     5341 agaatcttac tctccaatat ttgggacaac cagtaaatgc taatttgtca gtgactgtca
     5401 ctggccaaga cgaagcaagt ccgaaaacta ttttgggaat tgaagtaagt caggaaccga
     5461 aaaaagatta cctagttggt gatagcttag acttgtctga aggacgcttt gcagtggctt
     5521 atagcaatga caccatggaa gaacattcct ttactgatga gggagttgaa atttctggtt
     5581 acgatgctca aaagactggt cgtcaaacct tgacgcttcg ttaccaaggt catgaagtca
     5641 actttgatgt tttggtatct ccaaaagcag cattgaacga tgagtacctc aaacagaaat
     5701 tagcagaagt tgaagctgct aagaacaagg tggtctataa ctttgcttca ccagaagtaa
     5761 aagaagcttt cttgaaagca attgaagcgg ccgaacaagt gttgaaagac catgaaacta
     5821 gcacccaaga tcaagtcaat gaccgactta ataaattgac agaagctcat aaagctctga
     5881 atggtcaaga gaaatttaag gaagaaaaga cagaacttga tcgcttaaca ggtgaggttc
     5941 aagaactctt ggctgccaaa ccaaaccatc cttcaggttc tgccctagct ccgcttcttg
     6001 agaaaaacaa ggccttggtt gaaaaagtag atttgagtcc agaagagctt gcaacagcga
     6061 aacagagtct aaaagatctg gttgctttat tgaaagaaga caagccagca gtcttttctg
     6121 atagtaaaac aggtgttgaa gtacacttct caaataaaga gaagactgtc atcaagggtt
     6181 tgaaagtaga gcgtgttcaa gcaagtgctg aagagaagaa atactttgct ggagaagatg
     6241 ctcatgtctt tgaaatagaa ggtttggatg aaaaaggtca agatgttgat ctctcttacg
     6301 cttctattgt gaaaatccca attgaaaaag ataagaaagt taagaaagta tttttcttac
     6361 ctgaaggcaa agaggcagta gaattggctt ttgaacaaac ggatagtcat gttatcttta
     6421 cagcaccaca ctttactcat tatgcctttg tttatgaatc tgctgaaaaa ccacaacctg
     6481 ctaaaccagc accacaaaac aaagtccttc caaaacctac ttatcaaccg gcttctgatc
     6541 aacaaaaggc tcctaaattg gaagttcaag aggaaaaggt tgcctttcat cgtcaagagc
     6601 atgaaaatac tgagatgcta gttggggaac aacgagtcat catacaggga cgagatggac
     6661 tgttaagaca tgtctttgaa gtttatgaaa acggtcagcg tcgtcttcgt tcaacagaag
     6721 tcatccaaga agcgattcca gaaattgttg aaattggaac aaaagtaaaa acagtaccag
     6781 cagtagtagc tacacaggaa aaaccagctc aaaatacagc agttaaatca gaagaagcaa
     6841 gcaaacaatt gccaaataca ggaacagctg atgctaatga agccctaata gcaggcttag
     6901 ccagccttgg tcttgctagt ttagccttga ccttgagacg gaaaagagaa gataaagatt
     6961 aaatatcgaa aaatcttgtg aaatctttcc ttatatttcc aaagtgtgat ataatagttt
     7021 cgaataaaat aaataaagga gtttttgtaa catggcaaaa cttactgtta aagacgttga
     7081 cttgaaaggt aaaaaagtcc tcgttcgtgt tgacttcaac gtaccattga aagatggcgt
     7141 aatcactaac gataaccgta tcacagcagc tcttccaact attaagtaca tcatcgaaca
     7201 aggtggacgt gcaattcttt tctctcacct tggacgtgtg aaagaagaag ctgataaagc
     7261 tggtaaatca cttgctcctg tagcagctga cttggcagca aaacttggtc aagatgttgt
     7321 tttcccaggt gtcactcgtg gtgctgaatt ggaagcggca atcaacgctc ttgaagatgg
     7381 acaagttctc ttggttgaaa acactcgtta cgaagatgtt gacggcaaga aagaatctaa
     7441 aaacgatcct gaacttggta aatactgggc atcacttgga gatggtatct tcgtaaacga
     7501 tgcattcggt acagctcacc gtgcacacgc atctaacgtt ggtatctcag caaacgttga
     7561 aaaagcagtt gctggtttcc ttcttgaaaa cgaaattgcc tacatccaag aagcagttga
     7621 aactccagaa cgtccattcg tggctatcct tggtggttca aaagtttcag acaagatcgg
     7681 tgttatcgaa aacttgcttg aaaaagctga taaagtcctt atcggtggtg ggatgactta
     7741 cacattctac aaagcacaag gtatcgaaat cggtaactca cttgtagaag aagacaaatt
     7801 ggatgttgcg aaagctcttc ttgaaaaagc aaatggtaaa ttgatcttgc cagttgactc
     7861 aaaagaagct aacgcatttg ctggttacac tgaagtgcgt gacactgaag gtgaagcagt
     7921 ttctgaaggc ttccttggtc ttgacatcgg tccaaaatct atcgccaaat ttgacgaagc
     7981 tttgactggt gccaaaacag ttgtatggaa cggacctatg ggtgtatttg aaaacccaga
     8041 tttccaagct ggtacaatcg gtgtgatgga cgctatcgtg aaacaaccag gagttaaatc
     8101 aatcatcggt ggtggtgact cagctgccgc agcgattaac cttggccgtg cagacaagtt
     8161 ctcatggatt agtacgggtg gtggagcatc aatggaactt cttgaaggta aggttcttcc
     8221 aggacttgca gccctgacag aaaaataaga ttttataaac aaatcaaaga agagagggat
     8281 gaaagttcct cttttctttt gcttaaaata aaaacgcttc ctctcaacta ttactcataa
     8341 aaatcaccga tttatgataa aatggaaata gaaagttgag attatgagtt attttaaaaa
     8401 atataaattc gataaatccc agttcaaact tggtatgcga acctttaaaa caggtattgc
     8461 tgtttttcta gttctcttga tttttggctt ttttggctgg aaaggtcttc aaattggtgc
     8521 tttgacagcc gtttttagcc tgagggagag ttttgatgag agtgttcatt ttgggacttc
     8581 gcgtattcta ggaaatagta tcggtggact ctatgccttg gtctttttct tattaaatac
     8641 ctttttccac gaagcctttt gggtgacctt ggtagttgtt ccaatctgca ccatgttaac
     8701 cattatgaca aatgtagcca tgaataacaa agcaggggtt attggtggtg tagcagctat
     8761 gttaatcatt accctatcaa ttccaagtgg tgagacaatt ttgtacgtgt ttgtgcgtgt
     8821 attagaaacg tttatgggag tttttgtcgc aattatcgta aattacgata ttgatcgtat
     8881 tcgtctcttt ttagagaaaa aagaaaaata atgttacatt ttataacatt atcaattgac
     8941 gtttgtcttt ttttagacta taatagacag aaagaaggaa attgtaaatg aaggaaaaag
     9001 aatttcgccg aaatatggct gtttttccta tcggcagtgt tatgaagttg accgatttat
     9061 cggcgcgtca gattcgttat tatgaagatc aagagttgat caagcccgat cgaaacgaag
     9121 gaaatcgtcg catgtattcc ttgaatgaca tggatcgtct gcttgaaatc aaagattata
     9181 tctctgaagg ttataatatc gctgccatta agaaaaaata tgctgaacgt gaagcgaaat
     9241 ccaagaaagc ggttagtcag actgaagtac gtcgtgcact tcacaatgaa ctcctccaac
     9301 aggggcgttt tgcttcagta cagtcacctt ttggtcgcgg ttaggcaacc gcaagtagtc
     9361 atagattaag gagaaaactt atgccaatca cagctgcaga tattcgtcgt gaagtcaagg
     9421 aaaaaaatgt tacctttatt cgtcttatgt tctcagatat tttgggaacc atgaaaaacg
     9481 tcgaaattcc tgctacagat gaacagttag ataaggtctt gtcgaacaag gttatgtttg
     9541 atggatcttc tattgaaggt tttgtacgta tcaatgagtc ggatatgtac ttgtacccgg
     9601 acttggatac atggacagtc ttcccttggg gagatgaaaa tggaagtgtt gcaggtctga
     9661 tctgtgatgt ctatacaaca gaaggtgaac catttgcggg tgaccctcgt ggtaatttga
     9721 aacgagctct tcgtcacatg gaagaagttg gattcaaatc cttcaacctt ggtccagagc
     9781 cagaattctt cctatttaag ttggatgaaa atggggaccc aacacttgaa gtgaatgaca
     9841 agggtggcta ctttgacttg gcacctactg atcttgcgga caacacacgt cgtgagattg
     9901 tgaatgtctt gaccaaaatg ggatttgaag tagaagcgag tcaccacgag gttgcggttg
     9961 gacagcatga gattgacttt aagtacgatg aagttctccg tgcttgtgat aagattcaaa
    10021 tctttaagct tgttgttaaa accattgctc gcaaacacgg actttacgca acatttatgg
    10081 cgaagccaaa atttggtatt gctggatcag gtatgcactg taatatgtcc ttgtttgatg
    10141 cagaaggaaa taacgccttc tttgatccaa atgatccaaa aggaatgcag ttgtcagaaa
    10201 cagcttacca tttcctaggc ggtttgatca agcatgctta caactatact gctatcatga
    10261 acccaacagt taactcatac aaacgtttgg ttccaggtta tgaagcgcct gtttacattg
    10321 cttgggctgg tcgtaaccgt tcgccacttg tgcgcgtacc tgcttcacgt ggtatgggaa
    10381 ctcgtcttga gttgcgttca gtggatccaa tggcgaaccc ttacgttgct atggctgttc
    10441 ttttggaagt tggtttgtat ggtattgaaa ataaaatcga agcaccagct cctatcgaag
    10501 aaaatatcta catcatgaca gcagaagagc gcaaggaagc tggtattaca gaccttccat
    10561 caactcttca caacgctttg aaagctttga cagaagatga agtggttaaa gctgctctcg
    10621 gagatcacat ctatactagc ttccttgaag ccaaacgaat cgaatgggca agttatgcaa
    10681 ccttcgtttc acaatgggaa attgataatt atttagacct ttactaatac taatatagaa
    10741 gaaagattgc ctgtgggtaa acagtcacac agcagtctaa gccatcaaat acctataacc
    10801 cttaaaatag cgatttcggt cgctatttat ttttcgaaaa atcctcttga ctcttttgcg
    10861 aattagattt atcatcagac gtgtagaaga aaattaagga gtcactatgg aaaaattcaa
    10921 tttcaaaaac aatatcgggc aagaaaacaa gttacttcaa atcgaaatct acaagtttac
    10981 taacttttgc aagcttcaaa attacacatc tgtaaatatt ttttcgaaag atatatttga
    11041 agccatagta aattgagact agaatagtac actacatctg ctaaaaattt ctagaaacta
    11101 atttaacttt cctaatcgat ttgtttacat cttatttcaa tctactatat tatctcaata
    11161 aatgatgatg gcgactgttt tgtttgtgct tgtcgatttt tgaatcctga tcgagcggaa
    11221 gaatatttct gccgaggttg ccaaggggaa aatcctgaag atattgaatt ttatgatgaa
    11281 caattacagg cagaaaaggt ggaggttttg aatattcgat tggaagtaaa aaattgaatt
    11341 atctacaatc tcatcaacaa taaacctatt tcaaccccat tatctatagg cgtattttta
    11401 cgccttttta tatacttgaa gcacataaga aacattatgg gaagtttgct gatggaagta
    11461 ctcaagaaat tgatgttcct tatgatattc ctgatacttg ggagtgggtg aggataaaat
    11521 caatttattg gaattttggg caaaataagc cagagaaatc ctttaggtat atagatacgt
    11581 ctagtattga tagaaaaaag aacataatca actacaaaaa tctacaatat ctttcacctg
    11641 aacaagcgcc ttcccgtgct agaaaattag tttcgcagaa tagtgtctta ttttcaacag
    11701 ttagaccata tctaaaaaat attgctgtag ttagagaact taaagagtat ttgatagcta
    11761 gtacagcatt tattgttttg gatactttac ttaacgaaac atatttgaaa tattatttat
//
LOCUS       DAVLRT010000057        11598 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLRT010000057 DAVLRT010000000
VERSION     DAVLRT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.057"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            116..874
                     /locus_tag="VXM85_001547"
     CDS             116..874
                     /locus_tag="VXM85_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1346097.1"
                     /translation="MVLVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDRMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            914..1678
                     /locus_tag="VXM85_001548"
     CDS             914..1678
                     /locus_tag="VXM85_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1346098.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1691..2368
                     /locus_tag="VXM85_001549"
     CDS             1691..2368
                     /locus_tag="VXM85_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1346099.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGATLLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2378..3037
                     /locus_tag="VXM85_001550"
     CDS             2378..3037
                     /locus_tag="VXM85_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1346100.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3295..4485
                     /locus_tag="VXM85_001551"
     CDS             3295..4485
                     /locus_tag="VXM85_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1346101.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVSSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4806..5687)
                     /locus_tag="VXM85_001552"
     CDS             complement(4806..5687)
                     /locus_tag="VXM85_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1346102.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5826..7154)
                     /gene="vncS"
                     /locus_tag="VXM85_001553"
     CDS             complement(5826..7154)
                     /gene="vncS"
                     /locus_tag="VXM85_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1346103.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(7151..7807)
                     /gene="vncR"
                     /locus_tag="VXM85_001554"
     CDS             complement(7151..7807)
                     /gene="vncR"
                     /locus_tag="VXM85_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1346104.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7856..7939)
                     /gene="pep27"
                     /locus_tag="VXM85_001555"
     CDS             complement(7856..7939)
                     /gene="pep27"
                     /locus_tag="VXM85_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1346105.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(8119..9498)
                     /gene="vex3"
                     /locus_tag="VXM85_001556"
     CDS             complement(8119..9498)
                     /gene="vex3"
                     /locus_tag="VXM85_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1346106.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9550..10197)
                     /gene="vex2"
                     /locus_tag="VXM85_001557"
     CDS             complement(9550..10197)
                     /gene="vex2"
                     /locus_tag="VXM85_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1346107.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10210..11487)
                     /locus_tag="VXM85_001558"
     CDS             complement(10210..11487)
                     /locus_tag="VXM85_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1346108.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3269 a         2597 c         2196 g         3536 t
ORIGIN      
        1 gatcgatttt tcctgttctt atttcactat agtgaatttt ttcagatatg atttgacaag
       61 aacattcttt tattgtagaa ccgtgttata aaatataaca caatagagat ctctcatggt
      121 tttagtagaa tttaaaaacg tcgaaaaata ttacggagac taccacgcac tccgcaacat
      181 caatctccgt tttgaaaaag gacaagttgt tgtcctgctt ggaccttctg gctctgggaa
      241 gtccactctt atccgtacga tcaatggttt agaggctgtt gacaaaggaa gtctcctagt
      301 caatgggcac caagttgctg gtgccagcca gaaagatttg gtacctcttc gcaaggaagt
      361 cggcatggtt tttcaacatt ttaaccttta tccacacaaa acagtgttag aaaacgtgac
      421 acttgcgccc attaaagttc taggaattga taaaaaagaa gctgaaaaaa ccgccaaaaa
      481 atatctggaa tttgtaaata tgtgggacaa gaaagattcc tatcccgcca tgctatctgg
      541 tggacaaaaa cagcggatcg ccatcgctcg tggtcttgct atgcatccgg aactcctcct
      601 ctttgatgaa ccaacatctg ctcttgatcc tgagactatc ggagatgttc tagcagttat
      661 gcagaaactg gcgcgtgata ggatgaacat gatcatcgtt acccacgaaa tgggctttgc
      721 tcgagaggtt gcggaccgca ttatctttat ggccgacgga gaagttttag tagatacgac
      781 agatgtcgat aacttttttg acaatccaag cgaacctcgt gcccaacaat tcctcagcaa
      841 aattatcaac cacgaaagtg acaaagtcaa ataaggaggc gcctatgaaa aaaaattctt
      901 tttatcagca ttattgatta gccttttcgg ccttgctgct gccaaaccag tccaggctga
      961 tacaagtatc gcagacattc aaaaaagagg cgaactggtt gtcggtgtca aacaagacgt
     1021 tcccaatttt ggttacaaag atcccaagac cggtacttat tctggtatcg aaaccgactt
     1081 ggccaagatg gtagctgatg aactcaaggt caagattcgc tatgtgccgg ttacagcaca
     1141 aacccgcggc ccccttctag acaatgaaca ggtcgatatg gatatcgcga cctttaccat
     1201 cacggacgaa cgcaaaaaac tctacaactt taccagtccc tactacacag acgcttctgg
     1261 atttttggtc aataaatctg ccaaaatcaa aaagattgag gacctaaacg gcaaaaccat
     1321 cggagtcgcc caaggttcta tcacccaacg cctaattact gaactgggta aaaagaaagg
     1381 tctgaagttt aaattcgtcg aacttggttc ctacccagaa ttgattactt ccctgcacgc
     1441 tcatcgtatc gatgcctttt ccgttgaccg ctctattcta tctggctaca ctaataaacg
     1501 gacagcacta ctagatgata gtttcaagcc atctgactac ggtattgtta ccaagaaatc
     1561 aaatacagag ctcaacgact atcttgataa cttggttact aaatggagca aggatggtag
     1621 tttgcagaaa ctttatgacc gttacaagct caaaccatct agtcataccg cagattaagg
     1681 aggataccct atgacagatt tatcatcttg gacagcctat tttcaggatt ttggacaatt
     1741 tttcaatggt ttcctcttta ctcttgccct agcgattgga tcctttatcc tcgccatggt
     1801 cttaggaatc ttctttggtg ccatgtcaac cagtaaacgt ccaattttac gcattttagc
     1861 tcgcatcttt gtggaatttt accaaaatac tcctctcttg gtgcagtttg ttattgtttt
     1921 ttatggccta cctcttatca gtgaccatat catcatgatt ccaatttatt ggacagccgt
     1981 tctctgcgtg ggactctatc acggcgctta tatcgctgag gttattcgtt cagggattca
     2041 gtctattcct agtggtcaga tggaggccgc cttgtcgcaa ggttttacct atatcagtgc
     2101 catgcgcttg attaccttgc ctcagacctt ccgcatcatt ctccctccat tgaccaacca
     2161 aattgttaac ctcatcaaga acacctctac tgtagctatc atctctggag tagacttgat
     2221 gtttgtgact aagtcttggt cggctctcaa cggaaactat atcccagcct ttttaggtgc
     2281 tactcttctc tactttgccc tatgcttccc tgttgcccag tttggtcgca agatggagca
     2341 agccaacaaa aaagcctatt cactttagga ggttactatg gaatctattt tagaagtttt
     2401 aaccccagat aacctagtct ttatctttaa aggatttggc ttgaccctct atatttctct
     2461 gattgccatc atcctctcta ctatcatcgg tacggtgcta gctgtcatga gaaatggcaa
     2521 aaatcctgtc ttgcgaatca tctccagcat ttatatcgaa tttgtgcgca acgttcccaa
     2581 ccttctctgg atttttacta tctttttggt gttcaaaatg aaatccacac cagcaggtat
     2641 tacagccttt actctcttta catcagcagc cttggctgag attattcgag gcggtctcaa
     2701 tgccgtagac aagggacaat acgaagcagg aatgtcacaa ggcttcacct cagcccaaat
     2761 cctctactac atcattctcc cacaagccat ccgcaaaatg ctaccagcca tcatttctca
     2821 gtttgttacc gtgattaagg ataccagcct cctctactct gttatcgccc tacaagaact
     2881 ctttggagcc agccaaattc tcatgggccg ttatttcgaa ccagagcagg tcttcagtct
     2941 ttgcatcctg attgccctca tctacttcag ctttaaccta gcaatttcta gcctgtctca
     3001 tatgctagcc aaacgttggc aacaagctgc agaataatac tcttcgaaaa tctcttcaaa
     3061 ccacgttagc tctatctgca acctcaaaac agtgttttga gcaacttgcg gctagcttcc
     3121 tagtttgctc tttgattttc attgagtata aacagccact ctccagttca attggagagt
     3181 tttttgatga gagtagatat ttatagacct caatttgaca ccatacattt tctatgataa
     3241 aatgtaacaa atttatttac aacaaatcga aatctaaatg aaatggaatt ttctatgaaa
     3301 tcagtcaaag gactactctt tatcatagct agttttatct tgactctttt gacttggatg
     3361 aacacttctc cccaattcat gattccagga ctagctttaa caagcctatc tctgactttt
     3421 atcctagcca ctcgtctccc actactagaa agctggtttc acagtttgga gaaggtctac
     3481 accgtccaca aattcacagc ctttctctca atcatcctac taatctttca taactttagt
     3541 atgggcggtt tgtggggctc tcgcttagct gctcaatttg gcaatcttgc catctatatc
     3601 tttgccagca tcatccttgt cgcctattta ggcaaataca tccaatacga agcttggcga
     3661 tggattcacc gcctggttta cctagcctat attttaggac tctttcacat ctacatgata
     3721 atgggcaatc gtctccttac atttaatctt ctaagttttc ttgttagtag ctatgccctt
     3781 ttaggcttac tagctggttt ttatatcatt tttctatatc aaaagatttc cttcccctat
     3841 ctagggaaaa ttacccatct caaacgctta aatcacgata ctagagaaat tcaaatccat
     3901 cttagcagac ctttcaacta tcaatcagga caatttgcct ttctaaagat tttccaagaa
     3961 ggctttgaaa gtgctccgca tcccttttct atctcaggag gtcatggtca aactctttac
     4021 tttactgtta aaaattcagg cgaccatacc aagaatatct atgataatct tcaagccggc
     4081 agcaaagtaa ccctagacag agcttacgga cacatgatca tagaagaagg acgagaaaat
     4141 caggtttgga ttgctggagg tattgggatc acccccttca tctcttacat ccgtgaacat
     4201 cctattttag ataaacaggt tcacttctac tatagcttcc gtggagatga aaatgcagtc
     4261 tacctagatt tactccgtaa ctatgctcag aaaaatccta attttgaact ccatctaatc
     4321 gacagtacaa aagacggcta tcttaatttt gaacaaaaag aagtgcccga acatgcaacc
     4381 gtctatatgt gtggtcctat ttctatgatg aaggcacttg ccaaacagat taagaaacaa
     4441 aatccaaaaa cagagcttat ttacgaagga ttcaagttca aataagtaat catactctcc
     4501 tgggatttcc caggagagtt ttttatagcc aaatgaaata agacaggaac gatggaattt
     4561 tggcattcaa atcaattgat aagaatgttt tatagtggag tgaaatagga tacgaacaaa
     4621 ttgattagga aaatcaaatg aatttataga aatcttttag cagttatgtt gtcctattct
     4681 agtttcaaaa cgctatagaa gcagcattgt gctagtctag attcagttta ctatactaaa
     4741 acgagtagct tgaaatcaaa aaacccaccc tcacaggcag gttttatctg tattattcag
     4801 ctagattatg ctttaccttc tgaaccgaat acgtcgatac gttcttcaac cgatgcttgg
     4861 atagctttta caccgtcagc caagaattta cgtgggtcga agagtttttt cttgtcgtat
     4921 tctgcttcgt ttgcttcgta gtcacgagca aatttacgag ttgcgttagc gaatgcgatt
     4981 tggcattctg tgttaacgtt aactttggca acaccaagtt tgatagctgc ttggatttgc
     5041 tcatcaggaa tacctgatcc accgtgcaat acgattggga atcctggaag agcttctgtc
     5101 aatttttgca agtggtcaag gtctagacct tcccagttta ctgggtaagg gccgtggatg
     5161 ttaccgatac cagctgccaa gaagtcgata ccagtttcaa ccattgcttt agcgtcttcg
     5221 attggagcca attcaccttt accgatgatt ccgtcttctt caccaccgat agtaccaact
     5281 tcagcttcta ctgagatacc tttagcgtgt gctttttcaa caacttcttt agccaattta
     5341 aggttttctt caactggaag gtgtgaaccg tcaaacatga ttgaagtata accaacttcg
     5401 atacactcaa gtgcatcttc gtagtgaccg tggtcaaggt ggatagctac tggtacagtg
     5461 atacccattg attcaacaag gttagcgatc aagttgcgag caactttgta accacccatg
     5521 tatttagcag cacccattga agtttggatc aaaactggag cttttttagc ttctgctgcg
     5581 cgcaagatag cttgagtcca ctcaaggttg tttgtgttaa atccaccaac tgcataaccg
     5641 ttgtcacggg ctgcttggac aaatttttct gctgaaacga ttgccatttt atcaggcctc
     5701 ctgtatattt ttatgggtca tcccatttac attgttcatt ttatcacttt ttgccaaaaa
     5761 aatctagttt ttcccgcagt ttcgattgat tttcttctaa ctccatctat gtaaaccctt
     5821 tctccctagt cttggacgac ttttggaaaa tctataaaga aggttaaacg attctcctcc
     5881 atctcgaaac gataagctaa tttttcatgt tctaatagac tcttaaccac aaagagcccc
     5941 ataccagacc ccttgacctt gcgactagca ttgtcagaaa aagactgggc tagtttttct
     6001 tgttcctctg agctacagct attttcgata aaaagttctc cttctctttc tccaattcga
     6061 actaagccac ctggaacaga gtgcttaatg gcattgctga tgagattaga aagaatcaac
     6121 ttcataactg atgggtttag ataagcctgc tgatgggtca aactattgtc tatctggagc
     6181 tctctttcct ttgctagcaa ggcataatct ttgaccagat tttgcgtcat ctggaggagg
     6241 tcaattgttt ccctatcatc tcgcaattcc tgcacagaag agagggaaag tatctgcaga
     6301 acatggtgat tgagttcatc cacaatcccc aaggcaactc ccagatactg gtctctatcc
     6361 ttataacgac cgatattctc tctcatattt tcgattagga ttttcaaact agccagcggt
     6421 gttttcaatt catgagaagc tcctcgtagg aattcgacct tcatcttctc cagctggaga
     6481 atggcttcat tcttttcatg caagtccgca ataacagtca agagatgctg gtagaggcta
     6541 ttgatttgtt ccttgagatt acctatctca tccttagaat ccacgcgcaa tcgcacttgg
     6601 gaatccaggt ccatcatccg acgggtcacc cgcttgattt ccaaaatcgg tgcaacaata
     6661 gtccgagcgt agatgtaggc caccaagagg gaaatcagaa aggaggccag caaggtatag
     6721 ggaagaaact ggagactgat ttgctccgct tccttttgta aatccatgga agctagaaac
     6781 tggagaatca tagtaccacc gtcttgcgtt ttcacctcgc gctcctcaat aaagagagag
     6841 gttgtctggc ggtctgtgtc cagaggaaga ctgtccttga cttctaactt gtcctcggtc
     6901 atctcacctt tgacggtccc cttgatatca ctagtctggg aatacaagtc taacacttgc
     6961 tcgatactct gcctatcttt cccttctagg gactgggcaa tggctgttgc cttttgacca
     7021 atggtttcct gacgatgact cagataagtc gaaggaaaaa gaaaataaat agctaaatga
     7081 aggcagataa ccagaacact aaatatcgag aaggtataga taaatatctt tgcaaataaa
     7141 cctgttcgtt tcattttcgc tccaatttat aaccaacatt gcgcacagtg aggatacaat
     7201 ccaagtctag ctttttccgc aattccttga tataaacatc aataacacgg tcaaagggaa
     7261 cctcatctgt cgctttccag acggcatcga taatctgaga tcgagtcaag gcccggcctt
     7321 catttttcac tagatagtcc agaatttcca actctttggc attgataggc acttcttggc
     7381 ctgcgaggct tgcactgtag ctttcaaagt ccaccttggt atccttgtaa gaaaagattc
     7441 gtcctgtatc gtagtagcgc ttgaaaatcg cgtccaccct cacttttaaa agggagaggg
     7501 agaaaggttt ttccagatag ccatctgcca aagaggcaaa ggcactcatc ttgtattcct
     7561 catcttgaaa agctgtcaac atcaagacag gaacctgact ggttttacga atctcagcta
     7621 ggacttctaa gccgttgagc ttgggcatct ggatatccag taaaaccagg gccacctcat
     7681 agctagaaaa ttgctccaga gcttcctgac cgtccgctgc ctcaatagtt tcatagccac
     7741 aatccgtcaa ataatcactg accccctcac ggatcatctc ttcatcttct acaattaaaa
     7801 ttttcatact ttaactgctc tctatttttt atttttctta gaataaatac ctaccctatt
     7861 ttctattata gtctcttgct ggccttttgt ctgcaagcaa ctgaccacta gataaaacgt
     7921 tgtgaaattc ctttctcata aattccataa ctttagtata ttatatttaa gcactaaagt
     7981 acaaagaaag caactgaaag caatgatttt caccactgct ttcggattta ttttgaattg
     8041 ttaaatagcc attcctatcc actattcttg aatagaaaca caagatgcaa tctttattct
     8101 agactcattt tttcaaattt attcaccatc cagcaagagc tcttttggtt gttttctaag
     8161 gagattgctt gaagcaagcg ccataacgag aaccactaga accaaggcaa ggacaaaaat
     8221 gatgataaag tctgatgtct gaatggaaat gtctaggctc gacaaggtct tgctaaagcc
     8281 atctacttct gcaccaccac caaggttaga ggcttgagcc gccttactag cctgtttggc
     8341 aacacctgaa gtcacattgg caaggacagt gtttccaatt gcacgggcag tgtaattagc
     8401 taggaagtaa gcagaaacta gagcagggat agcaatcaag atagattcgg tgatgaattg
     8461 acccaagata cttgcctgct tgaggccgat agagaggaga attcccactt ccttgcgacg
     8521 ggcgttgatc caaaggctga gcaagagggc aaggaggaga actgagaagc tcaagctacc
     8581 ccagaagagg aggttggcca tcttgtacat accagagata gattgctcaa gagctgggta
     8641 gttagaggag ctcttgacga gtgtgtagct cttccagttg ataccactga tgccattcaa
     8701 ctctttcata acatcatcca agttcttgtc tgctgttaca aagaaggttg cgtccccata
     8761 aatggctgtg tcttctgtgt atccataaag ttttgcagca gtgtgaatgt ctgtaatagc
     8821 tgtgttttcg taaagttctt gtgagtaggt tactgctgac ttattatgac catcaaagag
     8881 tcccttgatt gtcacttcaa ctgtttcctt ggctcctttt tcattatctg catcgtagat
     8941 attagagtcc agtttaacct tgtcccctac tttccagccg tgtttggctg ccaagtcctt
     9001 gtgcaagagg attttatcct tgtcgtcgtt ggttaagtgc tctccttcga ctagtttata
     9061 agaaccagag acaaacttgt cttctttaga ggagtcattg acacctgtaa tcatcaagct
     9121 acttccaaaa cgcttggcac gatcagcagt gagattcttc ttggtttctg gcgtttcaat
     9181 caggtcatat ccagtcaaat ctccgatagc gttgatacgt ttgacataag actcaatggc
     9241 cttgttttcg gtgatttttt tgatgtcttc acccttgata ttcccagcac cacgaggcgt
     9301 tccttggttg acgcgacgat tgatttgcat ggagaagcta ttggtgatat ttttaaaggt
     9361 ctcctgagaa gccttggcag tagctccctt gattgacaag ccgaccaaac tcaagctcgc
     9421 catgaggaga ataatcagga agatgacaat cgatttgaaa aacttccttg taacataggc
     9481 aaatgcgttg tgtaacatag attccctttc tagattttgt tttaatcatt ctattaaaat
     9541 aagctcaaat tatttactag tattgcgcgt ttcagtcagt ttcttatcct ttaattcaag
     9601 tgtaatatct gacgcttgtg ccacttcttt actgtgagtt acgacaatca cacatttacc
     9661 tgttttctgg gcaagtgatt tgagtagttc gacaatatct ccagcagttt taggatccag
     9721 atttcctgtt ggctcatcag ctagaataac tggagcttct gagaccaaac tgcgagcaat
     9781 ggcaacacgt tgctgttgac cacctgataa ctggagaaca ttccgcttga tctggctttc
     9841 atccaaacca agctcaagaa gtgtattctt gcttgccttt ttgttgacca atcggatatt
     9901 ttccagcgga gaaagataat ctatcaagtt ataattttga aagaccaggg aaatatggtg
     9961 catgcgatgg taagaatagc ccttcttacg aatatcctct ccttgaaaaa ggatagaacc
    10021 ttcaacagga ctatctagac cagcaagtag ggacaagagt gtggattttc ctgctcctga
    10081 ctccccaata atactgtaaa attttccggg ttcaaaatta taattgatct gatataggac
    10141 tgcttcagca gtattcttat aacggtaggt aacatcttgt aattgtaata aagtcatgat
    10201 ttctccttct taactaatag atgataaaat ttctttcggt gattttctaa ataagaatag
    10261 gaaacaaagg gctacagata agcaactaag cagaactaga aaaacatagg attctgcaaa
    10321 agataagatg ctagttgata aactgcttgc tttggctagt gtatcttgta agcttgcctg
    10381 atctccactt gctagtagag tttggagtag gtaagttgtg attgcgtttc ctgcaacaaa
    10441 tgctggaagc aaagctccaa gagataccaa aactacctct aaacagaatt gtaggaagat
    10501 cgagctcttg ccttttccaa gtgcaagtaa aatccccact tcatagaccc gttctctcaa
    10561 ccagagagac aaaaccagaa ttaaggctcc agctcctgct atcaacatcc cataaaggaa
    10621 gatggtcagg aaggtttgga aagttgcaac tgagtctttg atttgttcaa aagccttgtt
    10681 ttccttttcg acttggtagc cttgattttc caaagccaag ttttctacct gcttcatgag
    10741 tccgtccatt tccttaggat tttctacata gaagcgtgct gcactgactt gagcttcact
    10801 attgcccaaa agggtttggc tactttcata gtctgtaaag acttgatttt cactgaagtc
    10861 agaagacaag cctgtgaatt tctcttgttt tttaccagaa aagatgccga taatctcaaa
    10921 ctctactgtt tgtccttttc cagattcaga ctgaccagca tccaagccaa tcttgtcatg
    10981 aagcgaaaga ccgttcttct tagccaattc ttcgtggata aggattttct tggaatcccc
    11041 tttttgaagg tgtcgccctt cttttagatt gaaagccgaa ctggtaaagg ttacatcctt
    11101 ggatgaatcc tcaagagccg ttaagctaac caagttattg tctgcagctg ataaatcatc
    11161 acgctccacg ctctgctcgc cagtcactgc ttccttgtct tttagttttg cgaccgtctc
    11221 aagttcagga gagacatttt ccagcccctt aatcttgctt acagatgcta ggtctgacaa
    11281 cttgaatgtc tgaccattct ctatcttctt aatagaaaaa gatgtattga gtgatttata
    11341 aagattgctt tctactgttt tgttggactt catcagagtc aaacaggctg aaattccggc
    11401 caataagacc aataaaatca gaaataaaat aaaacttctc agtcgctttc tgctgacata
    11461 agcccaagat ctttggattg gattcatttg tcacctccat atttgtaaga ctattataaa
    11521 acccaaatat gaaatgttta tgaaatacga aaaaaaaata tcgagtaggg gataatctct
    11581 agcccctctc acaccacc
//
LOCUS       DAVLRT010000058        11559 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLRT010000058 DAVLRT010000000
VERSION     DAVLRT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.058"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            356..568
                     /locus_tag="VXM85_001559"
     CDS             356..568
                     /locus_tag="VXM85_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346109.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            652..1455
                     /locus_tag="VXM85_001560"
     CDS             652..1455
                     /locus_tag="VXM85_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1346110.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGNGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(1511..3052)
                     /locus_tag="VXM85_001561"
     CDS             complement(1511..3052)
                     /locus_tag="VXM85_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1346111.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(3054..3536)
                     /locus_tag="VXM85_001562"
     CDS             complement(3054..3536)
                     /locus_tag="VXM85_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1346112.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(3536..4621)
                     /gene="rlmN"
                     /locus_tag="VXM85_001563"
     CDS             complement(3536..4621)
                     /gene="rlmN"
                     /locus_tag="VXM85_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV1346113.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMTIGARHITVSTLGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDSVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(4642..5172)
                     /locus_tag="VXM85_001564"
     CDS             complement(4642..5172)
                     /locus_tag="VXM85_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1346114.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(5328..5933)
                     /locus_tag="VXM85_001565"
     CDS             complement(5328..5933)
                     /locus_tag="VXM85_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1346115.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(6106..7143)
                     /locus_tag="VXM85_001566"
     CDS             complement(6106..7143)
                     /locus_tag="VXM85_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1346116.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(7177..8112)
                     /locus_tag="VXM85_001567"
     CDS             complement(7177..8112)
                     /locus_tag="VXM85_001567"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1346117.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(8665..9855)
                     /locus_tag="VXM85_001568"
     CDS             complement(8665..9855)
                     /locus_tag="VXM85_001568"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1346118.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(10070..11413)
                     /locus_tag="VXM85_001569"
     CDS             complement(10070..11413)
                     /locus_tag="VXM85_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1346119.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
BASE COUNT         3425 a         2543 c         2148 g         3443 t
ORIGIN      
        1 cattaaaaaa caccccatgg tgtgaaccat gaagtgttgt aaatgctgtg ttgtagctag
       61 ttcttagcgt ttagagaact ggctagcttt acgagctttc ttaagatctg gttccctaaa
      121 tactttgagt acacttatgg cattgatgcc ccaaaacatc taaaaactct ggtagaaaaa
      181 gggtatgctc ttgtcgaaac ttcttttgac tcacttgacc atctaaataa taccatgaaa
      241 aagaatatcc tgaagaagaa gaagggagtt actggacttt ctaaaatgaa atcttgcgac
      301 ctggatcaag cccttcatga gcatttttca gaagaagaat tagctggtca ctttcatgtc
      361 cttctatgga cttttttaca atggcattgc tatcacaccc aatacctatc taagcgcctg
      421 gttcgtaaac tttattgcag ctcttcctct aaatttccta attgttgaac caattgcccg
      481 ttttatacta agttcttttc agaaaccatt tactggggaa gaagttgaag attttcaaga
      541 tgatgatgaa atcccaacta ttatctaagc cagttctgta aactactaat atttgaaatc
      601 cacttccttt tagggtacaa tggttataaa tgaatttttg agaggatcag aatgaaaaaa
      661 ctagcaaccc ttcttttact gtctactgta gccctagctg ggtgtagcag cgtccaacgc
      721 agtctgcgtg gtgatgatta tgttgattcc agtcttgctg ctgaagaaag ttccaaagta
      781 gctgcccaat ctgccaagga gttaaacgat gctttaacaa acgaaaacgc caatttccca
      841 caactatcta aggaagttgc tgaagatgaa gccgaagtga ttctccacac aagccaaggt
      901 gatattcgca ttaaactctt ccctaaactc gctcctctag cggttgaaaa tttcctcact
      961 cacgccaaag aaggctacta taacggtatt accttccacc gtgtcatcga tggctttatg
     1021 gtccaaactg gagatccaaa agggaacggt acaggtggtc agtccatctg gcatgacaag
     1081 gataagacta aagacaaagg aactggtttc aagaacgaga ttactcctta tttgtataac
     1141 atccgtggtg ctcttgctat ggctaatact ggtcaaccaa acaccaatgg cagccagttc
     1201 ttcatcaacc aaaactctac agatacctct tctaaactcc ctacaagcaa gtatccacag
     1261 aaaattatcg aagcctacaa agaaggtgga aaccctagtc tagatggcaa acacccagtc
     1321 tttggtcaag tgattgacgg tatggatgtt gtggataaga ttgctaaggc cgaaaaagat
     1381 gaaaaagaca agccaactac tgctatcaca atcgacagca tcgaagtggt gaaagactac
     1441 gattttaaat cttaaaaacc aaaaaaatac agtatccaca ttcggtactg tatttctttt
     1501 actctcattc ttaagttaaa ttattaaaat cccatatttg gtctatccag ccttcataaa
     1561 agtctggctc gtggcagacc ataaggatag atcccctata ttctttgaga gcgcgtttga
     1621 gctcatcctt tgcatccaca tccaaatggt tggtcggctc gtccagcact aaaacgttgt
     1681 tttcacgatt catcaagaga cagaaacgaa ccttggcttg ctctccccct gataatactt
     1741 gaatctggct ttcaatatgt ttggttgtca aaccacaacg ggcaagggct gcacggactt
     1801 ctgcttgatt aagggcagga aaggcattcc agacagcttc aagaggagtt tggcgattac
     1861 cgccttctac ttcctgctca aaataaccaa gttctaaata atctccacgc tccacttccc
     1921 cagcgattgg cgagataatg cctaagagac tcttcaagag agttgttttt ccaataccat
     1981 tagcaccaat aatcgcaacc ttttgattgc gttcgaaggt aagatttaaa ggcttagtaa
     2041 gaggacggtc gtaaccaatt tgcaagttct tggcttggaa gataaagcgc cctggtgtac
     2101 gagctggttt gaaatcaaag gatggttttg gtttctcact ttggagttcg ataatatcca
     2161 tcttatccaa tttcttttga cgagacatag ccatattacg agttgcaaca cgggctttat
     2221 tacgagccac aaagtccttg aggtctgcaa tctctttctg ctggcgttcg taggctgcct
     2281 ctagctgaga tttcttcata gcataaactt cttggaactg gtagtagtca ccagagtaac
     2341 gcgtcagctg ttgattttcc acatgataga caatattaat aacgtcattg aggaatggaa
     2401 tatcgtgcga aatgagaaca aaggcattct catagttttg gagatagcgc ttgagccaat
     2461 caatatgctc agcatccaag tagttggtcg gctcgtccaa cagcaagata tcaggctttt
     2521 caaggagaag ttttgccaaa agcaccttgg ttctttgccc acctgacaaa gaagttacat
     2581 ccgtatccat gccaaagtcc ataacaccaa gagcacgcgc tacttcgtca atcttagcat
     2641 ccaaggtata gaaatcacga ctctccagac ggtcttgaag ttctcctact tcttccatga
     2701 gagcatcaac atccgcgccg tcttcagcca ttttcatata gaggtcattg atacgagctt
     2761 cagctttaaa aagctcatca aaagccgtac ggagaacatc acgcaccgac tgtctttcag
     2821 caaggacaga gtgctgatcc aagtaaccag ccgtcacata tttggaccac tcaacctttc
     2881 cttcatctgg cagcatttta ccagtcacga tactcataaa ggttgatttt ccttcaccat
     2941 tggcaccgac caggccgata tgttctccct tgaggagacg gaaggacaca tcttcaaaaa
     3001 ttgcacggtc accaaaaccg tgactcagat ttttaacttc taaaatactc attttaattc
     3061 cttatcttgt ttttatgtaa tcgtttataa aggagccaag ccagatagcc acccaaagtg
     3121 ttggtccaca aatcatcaat ctcaaagacg cgattgaaat caaagaaaaa gtccaagatt
     3181 aattgcgtac actcgattcc aagactcaca agaaaactaa aaagaaggac cttttttgtt
     3241 ttccgcaaat ttggaaatag ataaaggagt tggaaaatca gaggaaaaaa caagaagaca
     3301 ttgaggacat tttgtaaaaa aatccaacat aattgtccaa tgtcactcac ttcgcccagt
     3361 ttccagagag aattgaaagg agtcaaaaga aaaaccaggc gtccaagatg ctgaatacct
     3421 ggagttccca ctcccacggt agattgttct tgaggagtaa agcaaaaaca gacaatgcaa
     3481 atgctataga aaatgactcc ccagaccaaa atatgattat aagtcttctt catcattaag
     3541 gatttaccgc tgcgactgcc ttctggcggt cacgtttcat tgtgttagag cgcaattgtc
     3601 cacaagctgc gtcaatatct gtaccatgct cttgacgaac cacacagttg accccttttt
     3661 tcttaagcgt atcatagaaa gccaacacgc gctctttggg actacggcta tattggtcat
     3721 gctcactaac tgggttataa ggaatcaagt ttacataaga caatttcttg atgttcttga
     3781 gcaattcagt caattccaag gcttgttcta cactgtcgtt gacttcatta agcatgatat
     3841 attcaaaggt tacacgacgg tttgttgtct caatgtagta ttcaatagca gcaaagagtt
     3901 tttcaatcgg aaaggcacgg ttaatcttca tgatacttga acgaagttca ttgttaggtg
     3961 cgtgaagaga cacggcaaga ttgacctgaa ccccttcatc agcaaagtca cgaattttat
     4021 gagccaaacc taaggttgaa accgtgatgt gacgagcacc gatagtcatt cctttatcat
     4081 cattgatagt acgaaagaaa ttcaagacat tgttgtaatt atcaaagggc tcaccgattc
     4141 ccatgacaac gatatggctg acgcgttcat cctgaccacg ctcatcaaag tatttctgaa
     4201 ccagcatgat ttgcgctacg atttcaccgt tattgaggtc acgttgcttc ttaatcaaac
     4261 cagaggcaca gaaggtacaa ccgatattac agccgacctg agtggtcaca cagacagata
     4321 aaccatagtg ttgacgcatg agtacagtct caattaacat accgtcgggc aattcaaaga
     4381 gatatttgac tgtaccatca gcagactctt gcacaatacg ttgtttcaag ggattgacca
     4441 caaactggtc attgagctta gcaatcaaat ccttggaaag gttggtcatt tcttcaaatg
     4501 actgcacacg tttacggtag agccattccc agatttgatc tgcacggaat ttcttttctc
     4561 cctgctccaa tacccattcc tgcatggttt gatgtgccaa actatgaatt gagggtttca
     4621 tttcttctcc ttattctcta ctcacttctg acgaatgaca aaatgacgtt gtcccttgtc
     4681 ctctttctga gaatgctggt ctttctgacg acgtctattc ttcttatctg cattcgactt
     4741 tcgtttagtt tgagtcggtt tctttccttt tctagaaggt gtttcttctt ccgtcttacg
     4801 cattttcttg tcaaatgatg ctcgcttagg ggcttcattt tctaagacaa aataggcaca
     4861 accataacta caatactcta aaaggtagtc ttgtaaacga ctgatttttt caagtttttc
     4921 ttctgttcgg tcatccttgt aaaaacctcg taggcgaagc tgttcgttgc tccagtcccc
     4981 cacgatataa tcaaacttgg ttaatacttc tgaaaaacgc tgattaaaag tcgtcacatc
     5041 aaaggcatcc ttgatatttt caaccaagga aaaagctatc ccttccgttt cgaccttgtc
     5101 cccgtgtaaa tggaactccg gaccaggaaa cttgttatag ttgtataatt caggtgcaat
     5161 ttcttttcgc atagatatcc tttttccacg attacttaat actttattct accataattt
     5221 ctagcagtta gcacgtttct cataaaaatg aaaaaagtct gacgattttg tcagaccaga
     5281 atcttataac ctaaaaagag aagaacaatt cttccctcca actatcatta tttagcagct
     5341 gcgtacaatt catctacttt attccagttg attactgaaa agaaagcttt gatgtagtca
     5401 ggacgcacgt tgcggtattt cacgtagtaa gcatgttccc aaacgtccaa gcccaagatt
     5461 ggttttttac cttctgagat tggtgtgtct tggtttgctg ttgaagtcac ttcaagtttc
     5521 ccttctttgt tgacaaccaa ccatgcccaa cctgaaccaa aacgagttgt tgctgctgca
     5581 gtgaaggctg cttggaattc ttcaaatgaa ccaaatgttg catcgattgc tgctgccagt
     5641 tctgctgaag gagctgtttt ctcgggagtc atcaattccc agaaaagagc gtggttcaag
     5701 tgtccgccac cattgttgat aagtgcttga cggatatcag ctgggataga ttctacatca
     5761 gcaagcaagg cttcaaggtc ttcaccgatt tcagggtgtt tttctaaagc tgcattggca
     5821 ttgttgacat aagtttgatg gtgtttgtca tggtgcaaat gcattgtttc cgcatcgatg
     5881 tatggttcca aagcgtcgta tgcatatgga agttctggta agataatagc catctgtaat
     5941 acctcttttt ctttctatat gaaaatgata acgcaaacat tcactttttt caagtttttt
     6001 gcttatagat aaggaaatca ctgacatact ttctaataaa ataagcaaat cagcaagaaa
     6061 cccacgacaa gataatcctg acgcgggttg tagtttcaac aaatgtcaat tgacctgact
     6121 agcaatctgt aagagtgcct tttcaaaaag gaaacctttt tcataaagac ctgtcttaat
     6181 ctgatagtct gtctcaatca aataggaaat agcttgcttc agaaagctca aagaaagtcc
     6241 tctcgaatct cttaatgcaa acttgatttg ataaggattt gggttacgtc ccagataact
     6301 acctaaacta cttgcaatct gcgattctgt ttggccagac tccgccaaaa tcttcacctg
     6361 agtaaaagtc cgaaattgtc ccagcatgac tgcaatcagt ttgatttcat cttccccttg
     6421 caaggtcaag tctctcacca aatcgcgcgc ctgatccatc tttttagtca gaataaactg
     6481 agttaaatca aaaatattgt cctgcaaggt cttgggaatt gcgttaacaa tatcctcttc
     6541 ctcgataaca gaatccgcct tataggactg taaaaagaga agatttttct ggatttcgct
     6601 aaattgaaac cccgacttga tgaggagatt ttcaaaagaa tgattggtaa actgcagacc
     6661 ttgtttctga ctccactttt ggaaatactg gcgcaattct tgttctttta cttctactgc
     6721 atcgaagacc ttggcatcac gcttaagtaa tttgactaac cgtcttttgc tatccagctt
     6781 tccttctgca aagattatca acttggttgt tggagaagga ttgtcaaggt attcctcaaa
     6841 tgacttaagc tcatcatctg tcaaaaagcg tttcttagca gtcgtgatat ccataaaata
     6901 atctaatatc acgattttct catccgcaaa gaaaggaagg ctgaccaact ccagttccac
     6961 atccttgtaa actacttctt tcatatcaaa gtaggcaaag ttgaggtcag cagaatcata
     7021 cccaatctgt ttcaacactt gactcttcat cacttcaaac tgaccctgat ctgtccctgt
     7081 aaataggctc aggctcggta agtttgataa agtcaacttc tgactttctt caatggctag
     7141 catcgtctct cctttcttca gatttttcga tttaatttag tcaatatagc gcaatttccc
     7201 acggaaatct tctaagctct cgtagccttt ttccaccatg attgctttca gttcattggt
     7261 aatgcggtca aaagcactga cgccttcttt gtgaagggtc gttcccacct gcaccatact
     7321 tgctccacag aggatgtgtt caaaggcatc tcgaccagtc agaacgccac ctgttccgat
     7381 aatttggatt tgaggattta aacgttgata aaaggcgtga acattggcta gagcagtcgg
     7441 tttgatgtat tctccaccaa ttccaccaaa accattctta ggccgaataa cgacagattc
     7501 gtcttctata tagaggccgt ttccgataga gttaacgcag ttgacaaact tgagcggata
     7561 tttgttgaaa atagctgccg cttgatcaaa gtgaacaata tcaaaataag gtggcaattt
     7621 aattccaaga ggtttggtga agtaagcaaa cacttctgcc aaaatccggt ctgttgtctc
     7681 aaaatcatag gcaatctgag gtttacctgg aacatttgga caggaaagat ttagctcagt
     7741 cagaccacga aaatcactct cttggacttt tttcaaaata gtatgggttt cctctggaga
     7801 catgccgacc agagataaga agaaagttcg gttcgactct ttttcctgca aatctaaaag
     7861 ataatccaaa taatagtcta agccattatt tggcaagccc atagagttga tggaaccaag
     7921 tggaacatct tggtagcgtg gctcaggatt cccctgacgg aagtccaagg tcgctgtctt
     7981 agtaacaaag gttcctgccg ctgagttttt gacctcttct aactcctcta tcgtcataca
     8041 agccacacct gctgcattca tcaagcaatt gtcaaactca aaaccagcaa tttgtgtttt
     8101 cgttgatacc atgattctaa tcctccagat ttcttttatt gctaatatta taccataatt
     8161 ttaattctag gtttactaga ttctgtggtt ctatctattt attcggaaaa gaaagtctag
     8221 tacagaagtc atgctatgga aacagctagt gtattagaag tttgaacaag gcttgggtaa
     8281 cagacttttg aatgaagtcc aagcaacaag gagaaatctc aaccatgagt ccattccatg
     8341 actctgttgc tctagaaaac cttgcaatag tcaagtgata acgaataaaa ctttggattg
     8401 aaatatcccc aagattatct attacgagga gaaatcttaa cctttatcat ttttaaagaa
     8461 atatagtaaa atgaaacaag aacaggagaa atcgatcagg acagtcaaat tgattgctaa
     8521 caatgtttta gaagttgagg tgtactattc tagtttcaat ctactataca tgtaaatctc
     8581 tactttcgca aaatggattt tcagaacagt atcccagtat agttaaaaac tataaaaaag
     8641 agaggacgtt cccctcttaa aatcttattt tactgcttct ttcaaagcat ctaccttatc
     8701 caaacgttcc catggaagat caatatctgt acgtcccatg tgaccgtaag ccgatgtttg
     8761 acggtaaatt ggacgcttga ggtccagcat ttggataatc cctgcagggc gaaggtcaaa
     8821 gatttgacga gccgcttttt caagttgact ttcagctact gttcctgtac cgaaagtatc
     8881 gatacgaaca gaaacaggtt gcgcaacacc gatagcatag gccaactgca cttctgcctt
     8941 cttagcaagg cctgctgcaa cgatattctt ggcaatatag cgagccgcat aagaggctga
     9001 acgatccacc ttagtcgcat ctttaccaga gaaggcacca ccaccatgac gagagtagcc
     9061 accataagta tctacaataa tcttacgacc agtcaaacct gagtcccctt gaggaccacc
     9121 gattacaaaa cgacctgtcg gattgataaa gaattttgtc ttatcatcaa gataagaaga
     9181 tggaataact tctttgatga ccttgtcaat cacatcttga tggatttgtt cattagtggc
     9241 ctctggatca tgctgagtag aaataacgac tgtatctaca cgtaccggac ggtcattttc
     9301 atcgtactca actgtaactt gtgattttgc atctggacgg agatagctaa tttctccaga
     9361 cttacgaagt tctgccagac gacgaaccaa tttatgactg agtgcaattg gcaatggcat
     9421 aagctcttct gtttcatcta ctgcaaatcc aaacatgagc ccttggtcac ctgctccaat
     9481 caagtccagt ggatcttgat cagcatttcc acgaacctcc aaggcttcgt taacaccttg
     9541 agcgatgtca ggagattgtt ccaccaaaga tgggtgtact cccaccgtct cagcagaaaa
     9601 tccatattct gtattggtat aaccaatctc tgcaatggta tcacgaacca cacggttaat
     9661 atccacatag gcatttgtag aaatttcacc aaaaacgtgg acagaaccag tatatacagc
     9721 tgtttcagca gcaacgtgcg cctctggatc ctttgctaaa atagcatcca aaatcgcatc
     9781 tgaaatttgg tctgcaatct tatccggatg cccctcagat acagattcag acgtgaataa
     9841 tttacgttct gacataaaaa tgtcccccct taaaaaatat tgttatagag ttacaaagta
     9901 ctgataggaa aactcttcgt aataaatacc taccatctta tcgggactat ataactttat
     9961 cattatacca tttttaatcg caatttgcag tctttaatgt agtgtttcgg tttagatagt
    10021 tcgttctcat aaagcaaaaa gttggagctc agtactccaa caatttctat taaaatgttt
    10081 gacgtttagc tttacgctct gcacgaccgc gagcgcgatt ttcagcacgc ttggttttac
    10141 ggcgcttttc atcaaccgcc cattgaattt tcttcttata acccggtttg acttttttct
    10201 ttttcttttt aaccaaacca atcatttcga tatcaagttt atcttgtttt ttctcacggt
    10261 tggcacgacg atcacggtca taggtatctt gaaattcccc gtctttgacc atcttaggac
    10321 taaacttgat tcccaatttc tccaactcac ggatatccga gtcatcactt ggctgataaa
    10381 gggtaatagc tgtacctggt aggccatttc gtccagtacg accaacacgg tgaacaaaaa
    10441 aagataagtc ttgcggaatg gcatcattga tgacatggct gacaccttca atgtcaatcc
    10501 cacgtgctgc caaatctgtt gcgacaatat actcaaaatc cagattttgc acctgattca
    10561 tgattcgctt gcgttcacga ggggcaatat cgccatggat ttttgcaacc ttcaagcctt
    10621 gagcagtcag atatgaatgc aattcatcag cacgcgtttt agtgttaaca aaaatcattg
    10681 ccaaatacgg ctgcatcaac tgagtcaact ggtaaatttg agcattctta tcatgtccct
    10741 tggtcgaaat caaccaatta tcaatggtgt cagaaataac cgttttggtc ttaattttct
    10801 ccataacagg attggataag tattttttca agaatggttg cagtttttgt gggatagtcg
    10861 ctgagaagac catgaattgc aagtcttttg gaagactgcc agcaatctta tcaacagttt
    10921 ccaagaatcc catatccaag gtcatatccg cttcatcaac aacaaatgtc ttggctttat
    10981 gaatagctaa atcaccagat ttaaccaagt cgtagatacg gcctggtgtt ccaataacaa
    11041 tatgaggctg attgcttgcc aatttctcaa tctggcgagc cttatccgta ccacccacat
    11101 aattaaccac acgaacttcg acatctgagt gagctgaaat ctgacgcgct acttggtaaa
    11161 tttgagtagc caactcacga ctcggtgcag taatcactgc ttgtacacta tcgctagctt
    11221 catctaattg ctggaaaatc ggtaacaaga aagtatgagt cttacctgaa cctgtttttg
    11281 attctcctac taggtcacga cctgccaaaa caataggaat caacttgtct tgcacctctg
    11341 ttggagttgt aaattttaac tcctccaagg cttctctaat atagttttta aattgaaatt
    11401 tcgtaaatga cataacatcc tcgattctat ctatcttatc aattatacca tattttattc
    11461 cattacagta gtctcactta tttaggctat ttccagtagc ttctctagta agaaaaggct
    11521 ggaattttat accccaatta ttcaccccta atctaaaaa
//
LOCUS       DAVLRT010000059        11532 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLRT010000059 DAVLRT010000000
VERSION     DAVLRT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.059"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            114..767
                     /locus_tag="VXM85_001570"
     CDS             114..767
                     /locus_tag="VXM85_001570"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1346120.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            777..1241
                     /locus_tag="VXM85_001571"
     CDS             777..1241
                     /locus_tag="VXM85_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1346121.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1254..2522
                     /locus_tag="VXM85_001572"
     CDS             1254..2522
                     /locus_tag="VXM85_001572"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1346122.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALFLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2536..2796
                     /locus_tag="VXM85_001573"
     CDS             2536..2796
                     /locus_tag="VXM85_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346123.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2807..4168
                     /gene="pdrM"
                     /locus_tag="VXM85_001574"
     CDS             2807..4168
                     /gene="pdrM"
                     /locus_tag="VXM85_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1346124.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4322..5290
                     /locus_tag="VXM85_001575"
     CDS             4322..5290
                     /locus_tag="VXM85_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1346125.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5306..6298
                     /locus_tag="VXM85_001576"
     CDS             5306..6298
                     /locus_tag="VXM85_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1346126.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6516..7559
                     /locus_tag="VXM85_001577"
     CDS             6516..7559
                     /locus_tag="VXM85_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1346127.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     RVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7623..9308
                     /gene="lpdA"
                     /locus_tag="VXM85_001578"
     CDS             7623..9308
                     /gene="lpdA"
                     /locus_tag="VXM85_001578"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1346128.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELES
                     EEDGYLIAILKGEGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESSLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVITDKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9372..10361
                     /locus_tag="VXM85_001579"
     CDS             9372..10361
                     /locus_tag="VXM85_001579"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1346129.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10434..11504)
                     /gene="xerS"
                     /locus_tag="VXM85_001580"
     CDS             complement(10434..11504)
                     /gene="xerS"
                     /locus_tag="VXM85_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1346130.1"
                     /translation="MKREILLERINKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAINLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3391 a         2165 c         2617 g         3359 t
ORIGIN      
        1 gtattcaaat cgtatatata gtcgatttat taaatcttta gataaactgt aaaaagtggt
       61 ttccatagcc actttttgct ataatagagg cagtaaaaat gaaggagtcg gctatggaac
      121 actcgtcttg gcatgctttg attaaggcgc aattacctga gggttatttc gggaaaatca
      181 atcagtttat ggagcaggtc tattctcagg ggattattta tccacccaag gaaaaggttt
      241 ttcaggctct cttgacaaca ctgcttgaag aagttaaggt ggtaattcta gggcaagacc
      301 cctatcacgg accaggtcaa gcgcagggct tgagtttttc tgtacctgac tctatcccag
      361 ctccaccatc cttgcaaaat atcttgaaag aattgtcaga tgatatcgga gttaagaaat
      421 ctcatgattt gacagcttgg gctgagcaag gagtcttgct tcttaatgct tgtttgactg
      481 ttcctgctgg acaggccaat ggtcatgctg gtcaaatctg ggagcctttt actgatgctg
      541 tgattcaggt ggtcaatcat ctagatagac cagtcgtttt tgtactctgg ggagcttatg
      601 cacgtaagaa gaaggcatta gttaccaatc ctcatcactt gattatcgaa tcagcccatc
      661 caagtccttt gtcggtttat agaggatttt ggggttccaa gcccttttcc aaggccaatg
      721 cattcttaaa agagacagga caagagccaa tcgattggct tagataagga gaaaatatgc
      781 ctcagttagc gacgatttgc tacattgata atgggaaaga actgctcatg ctccatcgta
      841 ataagaaacc caatgatgtc catgaaggga aatggattgg tgtgggtggt aagctagaga
      901 gaggagagac gccccaggaa tgcgcggcgc gtgaaatcct tgaagaaaca gggctcaaag
      961 ccaagccagt tctaaaaggt gtcatcactt ttcctgaatt tacaccagat ttagactggt
     1021 acacctatgt ttttaaggtg acggagtttg agggcgactt gattgactgc aatgagggga
     1081 cgctagaatg ggttccctat gatgaggttt tgagcaagcc gacttgggaa ggtgaccaca
     1141 cctttgttga gtggctttta gaggataaac ccttcttttc agccaagttt gtttatgatg
     1201 gggataaatt gttggatacc caagttgatt tctatgaata aaggagaaag cagatgctac
     1261 taatcaaaaa tggtcgtgta atggatccca agtctggttt ggatcaagtt tgtgatgtct
     1321 tagttcaaga tgggaaaatt atcaaaattg cgcctgagat cacggaagaa ggagcagaaa
     1381 cgattgatgc tactggtctt gtggttgctc ctggtttggt cgatattcat gtgcatttcc
     1441 gtgaacctgg tcaaacacat aaagaagaca ttcatactgg tgccctggca gccgctgcag
     1501 gtggttttac tactgtcgtc atgatggcta ataccagtcc aaccatttca gacgtggaga
     1561 ctttgcaagc agttctccag tcagctgcca aagagaagat taatgtcaag acagttgcga
     1621 ccattactaa aaactttaat ggtaaaaact tgactgactt taaggcgctc ttagaagctg
     1681 gtgcggctgg tttctctgat gacggtattc cgcttgagag cagtaagatt gtcaaggaag
     1741 ccatggagga agccaaaaaa ctcaatacct ttatcagcct tcatgaggaa gatccaggtt
     1801 tgaacggtgt tcttggcttt aatgaaaata ttgctagaga acatttccat atctgcggtg
     1861 ctactggggt ggctgagtac gctatgatgg cgcgtgatgt catgattgcc tatgcaacta
     1921 aagcccatgt tcacatccag catttgtcta aggaagaaag tgttaaagta gtggagtttg
     1981 ctcaggggtt aggtgcagaa gtcacagcag aagtagcgcc acagcatttc tctaagacag
     2041 aagcactttt tttaacacaa ggtagcaatg ctaagatgaa tccaccgctt cgtttggaat
     2101 cagaccgtcg tgccgttatc gaaggtctca aatcaggtgt catcacagtt attgcgactg
     2161 accacgcgcc tcatcatgta gatgaaaaaa atgttgagga tattaccaaa gcgccatctg
     2221 gtatgactgg cttagaaaca tccctgtctc tcagcttgac ctatttagta gaagctggtg
     2281 agttgagctt gatggaatta cttgaaaaaa tgacatacaa cccagccaag ctttacaact
     2341 ttgaagcagg ttacttggct gagaatggtc cagcagatat cactattttt gatgccaagg
     2401 ctgaccgcct tgtggactcc cattttgctt ccaaagcagc taattcacca ttcatcggtg
     2461 aaaccttaaa agggcaggtt aaatatacca tctgtaaggg acaaatcgtc tatcaagctt
     2521 gataggagtt agaatatgaa ttattttcga aaacgtaggg agagacaagc gaaatctaat
     2581 agtggaattt actgtcccgc agctaatcgt tccaaaattc aatatgaaac taaagaaaaa
     2641 gctgaccatg cggttcaata tgataacgga ggtttagtta gaagctatta ttgtcgtaca
     2701 tgtgcgtgtt ggcatactac ttcaaaatct aataaacctc cgctaagttt gaaaaactat
     2761 ggtctgaaac tctttggttt agataaatca gaatagaaag agaagtatgt ataagacaaa
     2821 gtgtttacga gagaagttag tattattttt aaaaattttc ttcccaatcc tgatttacca
     2881 atttgccaat tattctgcct cttttgttga tactgcaatg acaggtcaat acaacactat
     2941 ggacttggct ggtgtatcta tggcaaccag tatctggaat cctttcttta catttctaac
     3001 agggattgtg tcagccttgg tgcctatcat tggtcaccat cttggtcgag gaaaaaagga
     3061 agaagttgcg tctgattttt accaatttat ttatttggcc ttgggcctat ctgtggtatt
     3121 gctggggatg gtacttttct tggcaccaac aatcttgaat catattgggt tagaagcagc
     3181 agtagcggca gtagcggttc gctatctttg gtttttatct atcgggatta tccccttgtt
     3241 gctctttagc gtcattcgtt ccttgctgga ttcgctgggc ttgaccaaac tgtccatgta
     3301 cctcatgctt ttgttactcc ctctcaatag cggatttaac tatctcttga tttacggtgc
     3361 ctttggtgtt ccagaactgg gaggggctgg tgctggttta ggaacatcct tggcctactg
     3421 ggtcttgctt gggatttctg ttctggtttt atttaaacag gagaagctca aagccttaca
     3481 ccttgagaaa cgaattccac ttaatatgga taaaattaag gaaggagttc gtttaggtct
     3541 gcctattggg ggaactgtct tcgcggaagt ggctatcttt tcagtggttg gcttgattat
     3601 ggctaagttt tcgtccttga ttatagctag tcaccagtca gctatgaact tttcaagtct
     3661 tatgtacgcc tttcctatga gtatctcatc ggctatggct attgtcgttt cctatgaagt
     3721 gggagccaag cgatttgatg atgcgaaaac ctatattggt ctaggaagat ggactgccct
     3781 catttttgcg gccttcacct taaccttcct ttacattttt aggggaaatg tggccagtct
     3841 ttatggtaac gacccaaaat ttatcgattt gacagtgcgt tttttaactt atagtctttt
     3901 cttccagtta gcagatacct ttgcggcgcc gcttcaggga attttgcggg ggtataagga
     3961 tacagttatt cctttttacc ttggtttgct tggttattgg ggcgtagcaa tccctgtggc
     4021 tacgctattt gattctctaa cagattttgg agcttattct tactggatcg gcttgattat
     4081 tagtttgatt gtgagtgggg cgctctaccg ttggcgttta actgtgatta tgaagagatt
     4141 tgaatcttta gcaaaatcca aacgctaaaa agtttgtgaa aaaatattct tgataagaaa
     4201 atcccttgtt ttcacggggt tttctttttt attttgtgaa atttctttta gaaaaacttt
     4261 gacagtattt gccaaaaacg gtaaaatagt aaggtaagaa gaaagttaga aaggcaagaa
     4321 gatgtcaact ttagataaaa atcttttgct agaaatgttc cgtaagatgg aagaaatccg
     4381 tcgcatggac ttaaaaattg cacaattagt aaagaaaggg aaagtgccag gaatgacgca
     4441 cttttctgtt ggtgaagagg cagctaacgt gggggctatg ttagctctca atccagatga
     4501 tctgattacc tcaaaccacc gtggacacgg gcaagctatt gctaaaggga ttgacctcaa
     4561 cggaatgatg gctgaaatcc ttggtaaata cactggaacc tgtaaaggga aaggtggatc
     4621 tatgcatatc gctgaccttg atgctgggaa ccttggtgcc aatggtattg taggtggtgg
     4681 tatggggatc gctgtcggtg cagccctcag tcagcaaatg caaaataccg gtaaaatcgt
     4741 tgtctgcttc tttggagatg gtgcgaccaa tgaaggtgtt ttccacgaag cagtgaacat
     4801 ggcttctatc tggaacctgc cagtcatttt ctattgcatt aacaacggtt acggtatctc
     4861 tgcggatatc aagaaaatga ccaatataga acatatccat caacgtagcg ccgcttatgg
     4921 aattcctgga atgttcatcg aagatggtaa caatgtcatc gatgtctatg aaggatttca
     4981 gaaagctgtg gaccatgttc gcagtggcaa tggtccagtc ttgattgaaa gtgtaactta
     5041 tcgctggctc ggtcactcat catctgaccc tggtaaatat cgtacgcgtg aagaagtgga
     5101 attgtggaaa caaaaagatc caatcgaaaa cctccgcaat taccttattg aaaataacat
     5161 tgcaagtgcc gaagaattgg aagaaatcca agcgcaagta aaggaagcag tagaagcttc
     5221 tgttaaattt gcagaggaaa gcccattccc accgcttgaa tcagcctttg aagatattta
     5281 cgcagactaa ggagaaagag ataaaatgga aacaaaaaca atgtccttcc gtgacaccat
     5341 tatccttgct atgtctgagg aaatgcgtcg cgatgaaaat gtgttcttga tgggagaaga
     5401 cgtcggtgtc ttcggaggag acttcggtac ttctgttgga atgcttgaag aatttggtcc
     5461 agaacgcgtc cgtgactgtc cgatttctga agctgccatc tctggagcag cagcaggagc
     5521 tgccatgaca ggacttcgtc caatcgtcga tatgaccttc atggacttct ctgttattgc
     5581 catggacaat atcgtcaacc aagctgctaa aacacgttac atgtttggtg gtaaaggtca
     5641 ggttccaatg actgttcgtt gtgcagctgg taacggagtt ggctctgccg cccagcactc
     5701 acaatctcta gagtcttggt ttactcacat tccaggactt aaggttgtgg cacctggaac
     5761 acctgcggac atgaaaggcc tgcttaagtc ttctatccgt gataaaaacc cagttattat
     5821 acttgagtac aagtcagaat ttaaccaaaa aggggaagtg ccagttgatc cagactacac
     5881 aattccactt ggggttggtg aaatcaaacg ccaaggaacg gatgtaacag ttgttactta
     5941 tggaaaaatg cttcgccgtg tggttcaagc tgctgaagaa ttagcagaag aaggaatttc
     6001 agttgaaatt gttgacccac gtacccttgt tccgcttgat aaggatatca tcataaactc
     6061 agtgaagaag actggtaagg ttgttctggt caacgatgcc cacaaaacaa gtggctatat
     6121 cggtgaaatt tcagctatta tttcagaatc agaagcattt gactatctag atgcaccaat
     6181 tcgccgttgt gcaggagaag atgtgccgat gccttatgca caaaacctag aaaatgcaat
     6241 gattccaaca gttgaaagta tcaaagatgc aatccgaaaa acttataaca aagaatagag
     6301 ttacataaga taagttatgt aaataaaata aagctgacga gaaactttgt atcttttagg
     6361 tgcagagctc ttcgtcatta atatcttaga ttagagcacg ggctaaaagc tcggaaaaaa
     6421 gataaatctc cttggcttca tcgaagccat cgtcgatttc ctatttttca gtcgcttttg
     6481 acgccctttg tatcataaat agaattggag aggtcatggc tgatgacaag ctaagagcga
     6541 ctcctgcagc tagaaagtta gcggatgatt tagggataaa cctctacgac gtttctggct
     6601 caggtgcaaa cggtcgtgtc cacaaagaag acgtggaaac ttataaagac acaaacgtgg
     6661 ttcgcatttc gccacttgca aaacgaattg ccctcgaaca taacattgct tggcaggaaa
     6721 tccaaggaac cggtcatcgt ggtaaaatca tgaagaagga tgttttggcc ctgcttcctg
     6781 aaaatatcga aaacgatagc atcaaatctc ctgctcagat tgaaaaagtg gaagaagtcc
     6841 ctgataacgt aacaccatat ggtgaaatcg agcgtattcc aatgacacca atgcgtaagg
     6901 ttattgccca acgcatggtt gaatcttact tgactgcgcc aaccttcacc ctcaactatg
     6961 aagttgatat gactgaaatg ttggctcttc gtaagagggt tcttgagcca atcatggaag
     7021 caactgggaa gaagactact gtaacagacc ttctttcact tgcagttgtt aagactctta
     7081 tgaaacatcc atacatcaac gcttcattga cagaagatgg caagactatt atcactcaca
     7141 actatgtcaa tcttgccatg gcagttggga tggataatgg attgatgaca cctgttgttt
     7201 acaatgctga gaagatgagt ctttcagaac tggttgtagc ctttaaggat gttattggcc
     7261 gtaccttgga tggtaaattg gctccaagtg agttacaaaa ttcaacattc acaatcagta
     7321 atttgggaat gtttggtgtt cagtcctttg gtccgattat taaccaaccc aactcagcta
     7381 tccttggtgt cagttcgaca atcgagaagc cagttgtcgt caatggtgaa attgtgattc
     7441 gcccaatcat gagtttagga ttaactattg accaccgtgt cgtagatggt atggctggtg
     7501 ctaagtttat gaaggacttg aaagagttga ttgaaacccc aatctcaatg ttgatttaag
     7561 agcaagagta ttcattttta agatatagat aaaggaagga agacatggcc ttagaagtaa
     7621 ttatgccaaa agccggcgtg gatatgacag aaggacaaat cgtccaatgg aataaaaaag
     7681 tcggagaatt tgtaaaagaa ggagaaatcc ttttggaaat catgactgat aaagtcagca
     7741 tggaattgga atccgaagaa gatgggtact tgattgccat tctcaaagga gagggtgaaa
     7801 ctgtccctgt aacggaagtt atcggttacc ttggtgaaga aagggaaaac atcccaacag
     7861 ctggagcagc atcgccagaa gctagccctg tacctgtagc aagtacctca aacgatgatg
     7921 gtaagagcga tgatgctttt gatatcgttg tgattggtgg aggtcctgct ggttatgttg
     7981 cagccattaa agctgcccaa ctcggcggta aggttgccct tgttgagaaa tctgaacttg
     8041 gtggaacctg cttaaaccgt ggatgtattc caaccaagac ctaccttcat aacgctgaaa
     8101 ttattgaaaa tatcggtcat gctgcaaatc gtggtatcgt gattgaaaat cctaacttca
     8161 ctgttgatat ggaaaaactt ttagaaacta aatctaaagt tgttaatact cttgttggtg
     8221 gtgttgcagg acttcttcgt agctacggag ttactgttca taaaggaatt ggtacaatca
     8281 ctaaagacaa gaacgtcttg gtaaatggtt ctgaattgct tgaaaccaag aaaatcattc
     8341 ttgctggtgg ttcaaaagtc agcaagatca acgtccctgg tatggaatct tcacttgtca
     8401 tgactagtga tgacattctt gaaatgaacg aagtgccaga aagccttgtt atcatcggtg
     8461 gtggagttgt cggtatcgaa ctcggtcagg ccttcatgac atttggttca aaagtgactg
     8521 ttatcgaaat gatggaccgt atagttccag ctatggatgc ggaagtttct aagaaccttc
     8581 gcttgatcct tgagcgtaaa ggaatgacca tcttgactgg tactaaactg caagaaatca
     8641 ttgaggaaaa tggtcaactt cgtatcaagg ttgaaggaaa agacgatatc atcgcaagca
     8701 aagctcttct ttcaattggt cgtatgccag accttgaagg tattggagag gttgagtttg
     8761 aattggatcg tggttgtatc aaggtcaacg aatacatgga aacttcagtt ccaggcattt
     8821 atgcaccagg tgacatcaac ggtactaaga tgttggctca cgcagctttc cgcatgggtg
     8881 aagtttccgc tgaaaatgcc cttaaaggaa atcatgcagt tgccaaattg aatttgactc
     8941 ctgcagccat ctacactctc cctgaagtag cagcagtagg tttgacagaa gaacaagccc
     9001 gtgagaaata cgatgttgcc atcggtaagt ttaactttgc tgctaacggt cgtgctattg
     9061 catctgacgc tgctcaaggt ttcgtaaaag ttatcactga taagaaatac ggagaaatcc
     9121 ttggtgtaca cattattggt cctgcagccg cagaattaat aaacgaagca tcaagcatca
     9181 tcgaaatgga aatcactgtt gaggaaatgc tgaagaccat ccacgggcac ccaacttact
     9241 ctgaagtgat gtacgaagcg tttgcagatg ttctaggaat ggccatccat tcacctaaga
     9301 aaaaataaaa caaagataga ttagactgga aggttatttc cagtttctat catataaagt
     9361 aaagggatat catgaaatac attatcaatc attcaaatga cactgctttt aatattgcct
     9421 tggaagaata cgcctttaaa caccttttgg atgaggatca aatcttccta ctttggatta
     9481 acaagccatc tatcattgtt ggtcgtcacc agaacactat cgaagaaatc aaccgtgatt
     9541 atgttcgcga aaatggtatt gaggtagttc gccgtattag tggtggtgga gctgtttacc
     9601 acgatttaaa taacctcaac tacacgatca tctccaaaga agatgaaaat aaggcatttg
     9661 acttcaagag cttctcaact ccggttatca ataccctggc tcaacttggg gttaaagctg
     9721 agttcacagg tcgtaatgac cttgagattg atggtaagaa gttctgtggc aatgcccaag
     9781 cctatatcaa cggccgtatc atgcaccacg gttgcttgct ctttgacgtt gatttgtcag
     9841 tcctcgcaaa tgcccttaag gtttcaaaag ataaatttga atcaaaaggt gtgaaatccg
     9901 tccgtgcccg tgtaaccaat attatcaatg aattaccaaa aaaaatcaca gtcgaaaaat
     9961 tccgtgattt actcttggaa tacatgaaaa aagagtaccc agagatgact gaatacgtct
    10021 tttcagaaga agaattggcc gaaatcaatc gcatcaagga tactaagttt ggaacttggg
    10081 actggaacta tggtaaatca cctgaattta acgtccgtcg tggaaccaaa ttcactagtg
    10141 gtaaggttga agtctttgct aacgttactg aatcaaaaat ccaagacatc aagatttatg
    10201 gtgacttctt tggtattgaa gacgttgctg cagtagaaga tgtccttcgt ggggtgaaat
    10261 acgaacgcga agatgttctt aaggcgctaa aaaccattga tatcacacgc tacttcgctg
    10321 gtattagccg tgaagaaatc gctgaagcgg tagttggata aatcaaaaag aagttggcca
    10381 actaaccaac ttcttttttt gatatttatt tacataattt aaaatacgta aaatcataaa
    10441 ctatccagag cattcttttg ttcatcatta acaatatggg tatagaggtc agtgacttgt
    10501 gtgctggcat gtcctagttg gtgactgact aaaacttgtg atttagtagc atcatagagc
    10561 ctagttgcta gtgtatggcg cagtttatgg ggtgttacac gtactttaaa atcctctgag
    10621 tatttagcaa ccattttctc aacgctagaa gcatcgatac gattaggaac acctctgtag
    10681 agagttaaaa aaagggctgt atctgttttt tccgttttat agcgttgatt ccgaatggcc
    10741 agataattct ctaaataagg tttagcaaaa gcagcgacat tgactgagtc acgtttgcca
    10801 ccttttcgag taacatcaat aaccatcatt tttagattga gatctcttag atctagatta
    10861 atagcttcag ataagcgaac accagatgcc aagagaaggg caataatggc taaatctcgt
    10921 tctttatttt tgttgaatga tgagagagct cgatttgaaa gctgttgtgg gtgctcttga
    10981 tcgatataag ttagaaaacc ttctgtttca tcacctagaa agagtttttg cttgatattt
    11041 tcagctctgg cagcaagggt ttctttcttt ttcttggttg aaactttttt cattacatta
    11101 cgatagaaat aaggttcccc ctgatcgttt tcaacctcct cggttagata cttgtaaaga
    11161 ctagaaagtg ctgataaggt tcgattgata gttgtctgtg aaacaccttg ttttgttgta
    11221 ttagcattca gcaagggacg ttcacgtaga taaaggataa aggattccat gtctttctta
    11281 gacatatttt ccaaaactga taaaggaata tcggatattt tatcagcgtt tgaaatacca
    11341 gactccaaaa cccagctgaa aaatcggtca tattccttaa ggtattcgta cagggttgta
    11401 aaactgtagg gcacagccag tttagattgg tagtattcca gaacatacca gggcatgagt
    11461 tgttttagtt tgttgattcg ttccagtaaa atctcacgtt tcatgtattt tctccataag
    11521 taagtatact ag
//
LOCUS       DAVLRT010000060        11505 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLRT010000060 DAVLRT010000000
VERSION     DAVLRT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.060"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            16..6090
                     /locus_tag="VXM85_001581"
     CDS             16..6090
                     /locus_tag="VXM85_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1346131.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVSDKG
                     VPEVQPELPEAVVSDKGVPEVQPELPEAVVSDKGEPEQVAPLPEYKGNIEQVKPETPV
                     EKTKEQGPEKTEEVPVKPTEETPVNPNEGTTEGTSIQGAENPVQPAEESTTNSEKVSP
                     DTSSENTGEVSNKPSDSTTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSN
                     QETEKPVQPAEETQTNSGKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSG
                     NTTSENGQTEPEPSNGNSTEDVSTKSNTSNSNGNEEIKQENELDPDKKVEDPEKTLEL
                     RNVSDLELYSLSNGTYKQHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKND
                     KILYKITAKVEKLQQEIESRYKDNFPFYLAKKGTEETTNFTSFSNLVKAINQNLSGTY
                     HLGASLNANEVELSTDDKSYIKGTFTGQLIGEKDGKHYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDSYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DRDAQIQDSYAEGDINNVKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKNEVVSKESFEERGTMLDASQIASKKAEI
                     NLITPPIVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKATIVKYGNLVK
                     ENSILYQKELLSAVMMKDDQVITDIISNKQTANKLLLHYKDHSSEKFDLRYQADFANL
                     AEYSIGDSGLLYTPNQFLYHQDSIINQVLPELNRVNYQSDAVRNTLGISPEVKLTELY
                     LEEQFTKTKEHLAENLKKLLSSDAGLVTDNEVMTGYIIDKIKRNKEALLLGMSYLERW
                     YNFSYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYAISLAS
                     HHGTTDLFSTLENYRKVFLPDKTNNDWFKSQTKAYIVEEKSNIEEVKTKQGLVGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTISSLGFGAYDRYRNKEHQANGDLN
                     SFVEKSAHETAERQRDHYDYWYRILDEKGREKLYRNILLYDAYKFGTNHTEGKATEVA
                     DFDSPNPAMKHFFGPVGNKVGHNGHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHNS
                     DQDIYLGGYGRRSGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSKEGERLQVL
                     DPTTRFKDATDLQKYVHNMFDVVYMLEYLEGKSIVKKLNVYQKIEALRKIENQYLTDP
                     ADGNDVYATNVVKNLTEDEAKKLTSFDSLIDNNILSAREYKAGTYERNGYFTIKLFAP
                     IFSALSGEKGTPGDLMGRRIAFELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPRYGTK
                     TINNVDELQKLMDEAVLQDAKERNYYYWNNYNPETDSAVHKLKRAIFKAYLDQTNDFR
                     RSIFENKK"
     gene            6134..>11505
                     /locus_tag="VXM85_001582"
     CDS             6134..>11505
                     /locus_tag="VXM85_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1346132.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGTSTYHYLDYS
                     TLTQTERVQLKQGRPDESKESYALVYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DKKRKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVDYLELDDR
                     TVSPVEQPKVVTDKGEPEVQPALPEAVVTGKGKTEVQPTLPEAVVTEKGEPEVQPVLP
                     EAVVAEKGEPEVQPVLPEAVVAEKGEPEVQPALPEAVVTEKGEPEVHEKPDYTQPIGA
                     NLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEPISTVSEVASEREELPS
                     LHTDIRTETIPKTTIEESDPSKFIGDNSVKQVGEDGERQIVTSYEELHGKKISESVET
                     VTILKEMKPEIIVKGTKERPKEKTAPVLTLTKVTEDAMNRSANLNYELDNKDNAEISS
                     IVAEIKDGDTVVKRVDLSKEKLTDAVQNLDLFKDYKIATTMIYDRGQGSETSKLDERP
                     LRLELKKVEIKNIASTNLVKVNDDGTETPSDFMNEKPSEEDVKKMYLKITSRDNKVTR
                     LTVDSIEEVTEEGQKLYKITAEAQDLIQHTDPTKVRNKYVYYIEKPHPKEDNVYYNFK
                     DLVDAMNTDKNGTFKLGADLNATGVPTPKKWYVDGDFRGTLKSVEGKHYTIHNTERPL
                     FQNIIGGTVTKVNLGNVNINMPWADRIAPIADTIKGGAKIEDVKVTGNVLGRNWVSGF
                     IDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVERAYVDANLIGKKAKAA
                     GIAYWTQNSGDNHKVGVEGAVKKGIVKGTIQVESPVEVGGAVGRLSHHGYIGEVVSMM
                     KVKKGEIFYGSSDMNDDPYWVANNVRGNYVVNGVSEGTISYARAKEHHRIKPISQSEA
                     DTKIMMLGITAQDFAINEPVVNRLNRLTRKEDEYKSTQDYKVDRDLAYRNIEKLQPFY
                     NKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGTKEEMDV
                     TKNADSKVKQVREYTIAGQNVVYTPNMVEKDRNQLIQDIKDKLASVQLISPEVRALMD
                     ARKKPEENTDERKNGYIKDLYLEESFAETKANLDKLVKSLVENADHQLNSDEAAMKAL
                     VKKVDENKAKIMMALTYLNRYYDIKYGDMTIKNLMMFKPDFYGKSVDLLDFLIRIGSS
                     ERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFTNDTDLNDWFIHAAKNVYVSE
                     PQTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAHMFLISTYNTMAYSSFEKYGK
                     YTEEARNEFKKEIDKVAHAQQTYLDFWSRLALPSVRDQLLKSENRVPTPVWDNQNYSG
                     IKGINRMGYDEKKVPIAPIRELYGPTWKFHNTNWNMGAMASIFPNPNNNDQVYFMGTN
                     MISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAYAQGMLQTPSSIGHQGEYGAL
                     GLNMAYHRENDGDQWYNYDPDKLQTREDIDRYMKNYNEALMMLDHVEADAVLPQLNGD
                     NSKWFKKIDREMRRNLGDGLNNLVAPHQWDNVRDLNQEESSKKLSSINDLIDNNFMTK
                     HGNPGNGRYRPEDFRPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYD
                     KGFTSYVSSKYQGEADKQNQGRLGDDFIIKKVSNNQFSNLEDWKKYWYHDVKSRAEKG
                     FTEITIDGQTIHNYNELKDLFDKAVTEDLKKAGNYSNTENLKSKVFKALLKNTDGFFN
                     PLFKKD"
BASE COUNT         4174 a         1873 c         2374 g         3084 t
ORIGIN      
        1 tttcaaggag gaataatgga aaagtatttt ggtgaaaaac aagagcgttt ttcatttaga
       61 aaattatcag taggacttgt atctgcaacg atttcaagtt tattttttat gtctgtatta
      121 gctagttcat ctgtggatgc tcaagaaact gcgggagttc actataaata tgtggcagat
      181 tcagagctat catcagaaga aaagaagcag cttgtctatg atattccgac atacgtggag
      241 aatgatgatg aaacttatta tcttgtttat aagttaaatt ctcaaaatca actggcggaa
      301 ttaccaaata ctggaagcaa gaatgagagg caagccctag ttgctggtgc tagcttagct
      361 gctctgggaa ttttaatttt tgctgtttcc aagaaaaagg ttaagaataa aacggtatta
      421 catttagtat tggttgcggg aataggaaat ggtgtcttag tttcagtcca tgctttagaa
      481 aatcatcttt tgctaaatta caatacggac tatgaattga cctctggaga aaaattacct
      541 cttcctaaag agatttcagg ttacacttat attggatata tcaaagaggg aaaaacgact
      601 tctgattttg aagtaagtaa tcaagaaaaa tcagcagcca ctcctacaaa acaacaaaag
      661 gtggattata atgttacacc aaattttgta gaccatccat caacagtaca agctattcag
      721 gaacaaacac ctgtttcttc aactaagccg acagaagttc aagtagttga aaaacctttc
      781 tctactgaat taatcaatcc aagaaaagaa gagaaacaat cttcagattc tcaagaacaa
      841 ttagccgaac ataagaatct agaaacgaag aaagaggaga agatttctcc aaaagaaaag
      901 actggggtaa atacattaaa tccacaggat gaagttttat caggtcaatt gaacaaacct
      961 gaactcttat atcgtgagga aactatagag acaaaaatag attttcaaga agaaattcaa
     1021 gaaaatcctg atttagctga aggaactgta agagtaaaac aagaaggtaa attaggtaag
     1081 aaagttgaaa tcgtcagaat attctctgta aacaaggaag aagtttcgcg agaaattgtt
     1141 tcaacttcaa cgactgcgcc tagtccaaga atagtcgaaa aaggtactaa aaaaactcaa
     1201 gttataaagg aacaacctga gactggtgta gaacataagg acgtacagtc tggagctatt
     1261 gttgaacccg caattcagcc tgagttgccc gaagctgtag taagtgacaa aggcgaacca
     1321 gaagttcaac ctacattacc cgaagcagtt gtaagcgata aaggcgtacc agaagttcaa
     1381 cctgaattac ccgaagcagt tgtaagcgat aaaggcgtac cagaagttca gcctgaatta
     1441 cccgaagcag ttgtaagtga taaaggtgaa ccagagcagg tagcaccatt gccagaatat
     1501 aaggggaata ttgagcaagt aaaacctgaa actccggttg agaagaccaa agaacagggt
     1561 ccagaaaaaa cggaagaagt tccagtaaaa ccaacagaag aaacaccagt aaatccaaat
     1621 gaaggtacta cagaaggaac ctcaattcaa ggagcagaaa atccagttca acctgcagaa
     1681 gaatcaacaa cgaattcaga gaaagtatca ccagatacat ctagcgaaaa tactggggaa
     1741 gtatccaata aacctagtga ttcgacaacc tcagttggag aatcaaataa accagaacat
     1801 aatgactcta aaaatgaaaa ttcagaaaaa actgtagaag aagttccggt aaatccaaat
     1861 gaaggcacag tagaagggac ctcaaatcaa gaaacagaaa aaccagttca acctgcagaa
     1921 gaaacacaaa caaactctgg gaaaatagct aacgaaaata ctggagaagt atccaataaa
     1981 cctagtgatt caaaaccacc agttgaagaa tcaaatcaac cagaaaaaaa cggaactgca
     2041 acaaaaccag aaaattcagg taatacaaca tcagagaatg gacaaacaga accagaacca
     2101 tcaaacggaa attcaactga ggatgtttca accaaatcaa acacatccaa ttcaaatgga
     2161 aacgaagaaa ttaaacaaga aaatgaacta gaccccgata aaaaggtaga agatccagag
     2221 aaaacacttg aattaagaaa tgtttccgac ctagagttat acagtttgtc aaatggtact
     2281 tataaacaac acatttcgtt agagcaagtt ccaagcaatc caaatagcta ctttgttaaa
     2341 gtgaaatctt cttcattcaa agatgtatac ctaccagtag catcaatatc agaggaaaga
     2401 aaaaatgata aaatccttta taaaatcaca gcaaaagtag agaagcttca gcaggagata
     2461 gaaagcagat ataaagataa ttttcccttc tatctagcta agaagggaac agaagaaaca
     2521 acaaacttta cttcctttag taatctggtc aaagctataa accaaaatct ctctggaacc
     2581 tatcatttag gagctagcct gaatgctaac gaagtggagc ttagtactga tgataaatcc
     2641 tatatcaagg gaacctttac tggtcagttg attggtgaaa aagatggtaa gcattatgct
     2701 atttacaact tgaaaaagcc tctttttgaa aacttgagtg gtgctacagt agaaaaactg
     2761 agtctaaaaa atgttgctat ttcagggaaa aatgatattg gttcactggc aaatgaagct
     2821 acgaatggca caaagattaa acaagttcat gttgatggtg ttctggctgg agaacgtggt
     2881 gtcggtggtt tgttggctaa ggcagaccaa tcaagcatcg cagagagcag tttcaaggga
     2941 agaattgtca atacctatga aacgactgat tcctacaata ttggcggtct ggtcggtcat
     3001 ttaacaggaa aaaatgcgtc tattgctaaa tccaaagcga cagtaaccat ttcgtcaaac
     3061 accaataggt cagatcagac tgtcggtggt cttgcaggtc tagtagaccg agatgcacag
     3121 atccaagata gctatgctga aggtgatatc aacaatgtca agcactttgg tagagtcgct
     3181 ggagtggcag gcaatttgtg ggatcgaact tctggtgatg ttaggcatgc tggaagtttg
     3241 accaatgttc tcagcgatgt taatgtaaca aacggaaatg ccatcactgg ataccactat
     3301 acaggaatga aggtagctaa tacatttagt agtaaggcta atagagtttt caatgtcact
     3361 ttagagaaga atgaggtcgt cagcaaagaa tccttcgagg aaagaggaac aatgctagat
     3421 gcttctcaaa ttgcaagcaa aaaagcagaa atcaatctta tcactccacc aatagtggaa
     3481 cccctctcaa caagcggtaa gaaagacagt gatttttcta agatagccca ttatcaagcc
     3541 aaccgtgctt tggtttataa gaacattgaa aaattgttac ctttctataa caaggcgacc
     3601 atcgtgaaat acggaaacct ggttaaggag aacagtatct tatatcaaaa agaactcttg
     3661 tctgcagtta tgatgaagga tgaccaagta atcacagata ttatttccaa caaacagact
     3721 gcaaacaaac tcttgcttca ctacaaggac cattcatctg aaaagtttga tctcaggtac
     3781 caggctgatt ttgccaattt ggcagaatat agcattggag atagtgggct tctctatacg
     3841 ccaaatcaat tcttgtatca tcaagattct attattaacc aagtgctgcc tgaattgaat
     3901 cgagtaaatt atcaatcaga tgctgttaga aatacacttg gaatttcacc agaagtgaag
     3961 ctaacggagt tatatcttga agaacaattt accaaaacga aagagcatct agcagagaac
     4021 ttgaaaaaac ttttatcatc tgatgcaggt ttagtaacag acaatgaagt tatgacagga
     4081 tatataatag ataaaattaa acggaataag gaagccttac ttcttggaat gagttattta
     4141 gaacgttggt ataactttag ctatggtcaa gtgaatgtca aagacctagt tatgtatcat
     4201 ccggacttct ttggtaaagg aaatacttca ccattagata ctctgattga gttaggtaaa
     4261 tctggcttta acaatcttct tgctaaaaac aatgtcgata cttatgctat cagccttgcc
     4321 agccatcatg gaacgacaga tttgtttagc acgttggaaa attaccgaaa agtctttcta
     4381 ccagataaaa ccaataatga ctggtttaaa tcacagacta aggcttacat tgtcgaagaa
     4441 aaatccaata tcgaagaggt gaaaacgaag caaggactag ttggcactaa gtattctatt
     4501 ggtgtctatg accgtatcac tagtgccaca tggaaatacc gtaatatggt actacctcta
     4561 cttaccttgc cagaaagatc cgtatttgtc atttcaacca tatctagtct aggttttggt
     4621 gcttatgatc gctatcgtaa taaagaacat caagcaaatg gtgatcttaa tagctttgtt
     4681 gaaaagagtg ctcacgaaac agcagagcgt caacgagatc actatgatta ttggtatcgt
     4741 attctagatg aaaaagggcg tgaaaagctt tatcgaaata tcttgcttta tgatgcctat
     4801 aaatttggaa ctaatcatac tgaaggaaag gctacagaag tagcagattt tgatagtcca
     4861 aatcctgcaa tgaaacattt ctttggacct gttggaaata aggttggaca taatggacat
     4921 ggtgcttatg ctacagggga tgcagtatat tatatggggt atcgcatgct agataaggat
     4981 ggagctatta cgtatactca tgagatgacg cataattcag atcaagatat ttatcttggt
     5041 ggttatggaa gaagaagtgg acttggacct gagttctttg ctaaaggctt actacaagca
     5101 ccagaccaac caagtgatgc aacgattact atcaattcta tattgaaaca ttcaaaatct
     5161 gatagtaaag aaggagaacg attacaagta cttgatccaa ctacgagatt taaagacgca
     5221 acagatcttc aaaaatacgt ccacaatatg tttgatgttg tttatatgtt ggaatatctc
     5281 gaaggtaaat ctattgttaa gaaattaaat gtatatcaga aaatagaagc tctcagaaaa
     5341 atcgagaacc aatatctaac agatcctgct gatggaaatg atgtttatgc tactaatgtt
     5401 gtgaaaaatt tgactgaaga tgaagctaaa aaattgacga gctttgatag tttgattgac
     5461 aataacattc tttcggctcg tgagtataag gctggcactt atgagagaaa tggctacttt
     5521 actataaaac tctttgcacc aatcttttca gcattgagcg gtgagaaagg aactccaggt
     5581 gaccttatgg gacgaaggat agcttttgaa cttctagctg ctaaaggatt taaagatggt
     5641 atggtaccat atatttccaa ccaatacgaa gaagatgcta aacaacaagg gcaaactatc
     5701 aatctttatg gtaaagaacg gggcttggtg accgatgagc ttgttttgaa aaaggtattt
     5761 gatggtaagt ataaaacttg ggcagaattt aagacagcta tgtaccaaga acgggtggat
     5821 cagtttggaa acttgaagca ggtgaccttt aaagatccga caaaaccatg gccaagatat
     5881 ggcacaaaga ctatcaataa tgtggatgaa ttgcagaaac taatggatga agctgttttg
     5941 caggatgcaa aagaacgtaa ttattattat tggaataact ataatccaga aacggacagt
     6001 gccgtccata agttgaagag agcaatcttt aaagcctatc ttgaccaaac aaatgatttt
     6061 agaagatcaa tttttgagaa taaaaaatag tgtttactat taggaaataa aaagtgaatg
     6121 tggaggatta gaattgtcat tattaaaaaa agataaattt tccattcgga aaataaaggg
     6181 tattgttggt tcggtattct tggggagtct tttatttgca ccgtcagttg ttggtacatc
     6241 gacttatcat tacttagatt atagtacttt gacacaaact gaacgtgttc aactcaaaca
     6301 aggtagacct gatgaatcaa aagagtcata tgctttggtt tatgaaaaag acgcattacc
     6361 aaacacagga agttctcaat ctattatgac tgcattgggt ttgttagcta tcgggagtct
     6421 tattgtgatt atcacaaaag ataaaaagag gaaaaaaatc gctacatttt tgattgtagg
     6481 ggcgacaggt ttagttacac tatcaactgc ttcagcactt aatttgaatg ctaacatcca
     6541 tgagtctgga cgtgatggtg tgttgcaaat ttctggttac agatatgttg attacttgga
     6601 gcttgatgat agaactgttt ctccagttga gcaacccaaa gttgtgaccg acaaaggcga
     6661 accagaagtt cagccagcgt taccagaagc tgttgtaact ggaaaaggga aaactgaagt
     6721 acaaccgaca ttaccagaag ctgttgtgac tgaaaaaggg gaaccggaag tacaaccagt
     6781 gttaccagaa gctgttgtgg ctgaaaaagg ggaaccggaa gtacaaccag tgttaccaga
     6841 agctgttgtg gctgaaaaag gggaaccaga agtacaacca gccttacccg aagcagttgt
     6901 aactgaaaaa ggcgaaccag aagttcatga gaagcctgac tatacgcaac caataggtgc
     6961 aaaccttgtt gagcctgaag ttcatgagaa actagcatac actgaacctg taggtacaac
     7021 aggagtagat gaaaatggaa acttgattga gccacctgtt aacgatattc cagagtatac
     7081 tgaaccaata tctacagttt cagaagttgc atcagaaaga gaagagttgc cttctttaca
     7141 taccgatatt cgtacagaga ctattcctaa aaccactata gaagaatctg atcctagcaa
     7201 atttataggc gataattctg ttaaacaagt tggtgaggat ggcgaacgtc aaattgttac
     7261 tagctatgaa gagttacatg gcaaaaaaat cagcgagtca gttgaaacag taacaatttt
     7321 gaaagaaatg aagcctgaaa ttattgtaaa aggtacaaaa gaaagaccaa aagaaaaaac
     7381 ggctcctgtt ttaaccttga ccaaagtaac agaagatgct atgaatcgtt ctgccaactt
     7441 aaattatgag cttgataata aagataatgc tgaaatatca agtattgtag ctgaaattaa
     7501 agatggtgat acagtagtta aaagggttga tttatctaaa gaaaaattaa ctgacgctgt
     7561 acaaaatttg gatttgttta aagattataa gattgcaaca acaatgattt atgatcgtgg
     7621 tcaaggttct gaaacgtcta aactggatga aagacctctg cgtttggagt tgaaaaaggt
     7681 cgaaattaaa aatatcgcat ctactaattt agtgaaggta aatgatgatg gtactgaaac
     7741 acctagtgat tttatgaatg aaaaaccttc agaagaagat gttaaaaaaa tgtacttaaa
     7801 aatcactagt cgcgataata aggtaactcg tttaactgta gactctattg aagaagtgac
     7861 tgaagaaggt caaaaacttt acaaaataac tgctgaagca caagatttga ttcaacacac
     7921 agacccaacg aaggttcgta ataagtatgt ttattatata gaaaaacccc atcctaaaga
     7981 agataatgtc tactataact ttaaagattt agtagatgct atgaatactg ataaaaatgg
     8041 tacatttaaa cttggtgcag atttgaatgc cactggtgtt ccaacaccta agaaatggta
     8101 tgttgacggc gactttagag gtacattgaa gagtgtagag gggaaacatt atactatcca
     8161 caacacggag cgtccgttat tccagaatat tattggtggt acagtaacca aagtaaatct
     8221 gggaaatgta aatatcaata tgccatgggc agaccgcatc gcaccgattg ctgacactat
     8281 taaaggtggc gctaaaattg aagatgtcaa agtgactggt aacgtattag gtagaaactg
     8341 ggtatctgga tttattgata agattgacaa ccaaggtaca ctcagaaatg tagcctttat
     8401 cggaaatgtg acatctgtag gtgatggtgg tcaattcttg accggtatcg ttggtgagaa
     8461 ctggaaagga ctagtcgaac gtgcgtatgt tgacgcaaat cttattggca agaaagccaa
     8521 ggctgctggt attgcttatt ggacacaaaa cagtggtgac aatcataaag ttggtgtcga
     8581 aggtgcagtc aagaaaggaa ttgtgaaggg taccattcag gtagaaagtc ctgttgaagt
     8641 cggcggtgct gttggacgtt tgagtcatca tggttatatc ggggaagtcg tatctatgat
     8701 gaaagttaag aagggtgaaa ttttttatgg ttctagtgat atgaatgatg acccatattg
     8761 ggtggcgaat aatgtacgag gaaattatgt agttaatggt gtgagtgaag gtacgatatc
     8821 ttatgctcgt gctaaagaac accatcgtat caaaccaatt tcacagtcag aagcagatac
     8881 taaaattatg atgttaggta ttacagcaca agattttgct atcaacgaac ctgtggtcaa
     8941 tcgcttaaac cgtttaacca gaaaagaaga tgaatataag tcaacccaag actataaagt
     9001 agatcgagat ctggcttatc gtaatattga gaaacttcaa ccattttata acaaagaatg
     9061 gattgtcaac caaggtaaca aacttgcaga agattcaaac ttagctaaga aagaagtcct
     9121 ttctgttacg ggtatgaagg atggacagtt tgtaactgac ttatcagata ttgataagat
     9181 tatggttcac tatgctgatg gtactaaaga agaaatggac gtcactaaga atgctgattc
     9241 taaagtgaaa caagttcgtg aatataccat cgctggacaa aacgttgttt atacaccaaa
     9301 tatggttgag aaagaccgta accaattgat tcaagatatc aaagataagt tggcgtcagt
     9361 tcaattgatt tctcctgaag tacgtgcttt gatggatgca cgtaagaaac cagaagaaaa
     9421 tactgatgaa cgtaagaatg gttatattaa agacctttac ttagaagaaa gctttgctga
     9481 aacgaaagct aatcttgata aattggttaa atccttagtt gaaaatgcag atcatcaatt
     9541 aaatagtgac gaagcggcta tgaaagcact tgttaagaag gtggatgaaa ataaagctaa
     9601 aatcatgatg gctctgactt atctcaatcg ttactatgat atcaagtatg gggatatgac
     9661 tatcaagaac ttgatgatgt ttaaacctga tttctatggt aaatcagttg accttcttga
     9721 tttcttgatt cgaattggat caagtgagag aaatatcaaa ggtgacagaa ctttggatgc
     9781 ttatcgtgat atgattggtg gaactattgg taagtctgaa ttacatggat tcttagatta
     9841 caatatgcgt ctcttcacta acgatactga tttaaatgat tggttcatcc atgcagctaa
     9901 gaatgtatat gtatcagaac ctcaaacaac gaacccagat tttgttaata aacgacatcg
     9961 tgcatttgat ggtttgaaca acggtgttca taatcgtatg attctacctc tattaactct
    10021 taagaatgca catatgttct tgatttcaac ttacaataca atggcttata gttcttttga
    10081 gaaatatggt aagtatacag aagaagctag aaatgaattc aaaaaagaaa ttgataaggt
    10141 tgcacatgct caacaaacat acttagactt ctggtcacgt ttagcattgc caagcgtacg
    10201 tgaccaatta ctcaagagtg agaatagggt accaactcct gtgtgggata accaaaacta
    10261 tagtggtatt aaaggaataa accgtatggg ttatgatgag aagaaagtac caattgcgcc
    10321 aattcgtgag ttatatggtc caacttggaa attccataat accaactgga atatgggagc
    10381 tatggcatct attttcccaa atcctaataa caatgaccaa gtttatttca tgggaacaaa
    10441 tatgattagt ccgtttggta tttcagcctt tactcatgaa actacacacg ttaatgatag
    10501 aatgctttac ttcggtggtc atagacaccg tcaaggtaca gacgtcgaag cctatgctca
    10561 aggtatgcta caaactccgt caagtattgg tcaccaaggt gaatatggtg ctttaggttt
    10621 gaacatggct tatcatcgtg aaaatgatgg tgaccaatgg tataactatg accctgataa
    10681 gcttcaaact cgtgaagata tcgatcgata tatgaaaaac tataatgaag cactgatgat
    10741 gctagatcat gtagaagcag acgccgtact tccacaattg aatggtgata atagcaagtg
    10801 gttcaagaaa attgatagag aaatgcgcag aaatcttggg gatggtttga ataacttagt
    10861 agcaccgcac caatgggaca acgttcgtga cttaaatcaa gaagagagtt ctaagaaatt
    10921 atcaagtatt aacgatttga ttgataataa tttcatgacc aaacacggaa atccaggtaa
    10981 cggacgttat agaccagagg attttagacc aaactcagct tacgtaaatg tcaatatgat
    11041 ggcaggaatt tacggtggga atacgagtca aggtgctcct ggttcattat cgttcaaaca
    11101 taatgccttc cgtatgtggg gttattatgg atatgacaaa ggtttcacta gttatgtatc
    11161 tagcaaatat caaggtgaag ctgataaaca aaaccagggt agattaggtg atgatttcat
    11221 tatcaaaaaa gtttcaaaca accaattctc aaatcttgaa gattggaaga aatattggta
    11281 tcatgatgtg aaatccagag ctgaaaaagg attcacagag attacaattg atggacaaac
    11341 cattcacaac tataatgaac tgaaagactt attcgacaaa gctgtcacag aagacctcaa
    11401 gaaagctgga aactactcaa atactgagaa tttgaaatcc aaagtcttca aagctcttct
    11461 taaaaacaca gacggtttct ttaatccatt gttcaaaaaa gacat
//
LOCUS       DAVLRT010000061        10955 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLRT010000061 DAVLRT010000000
VERSION     DAVLRT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10955
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.061"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            14..736
                     /locus_tag="VXM85_001583"
     CDS             14..736
                     /locus_tag="VXM85_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1346133.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            733..1581
                     /locus_tag="VXM85_001584"
     CDS             733..1581
                     /locus_tag="VXM85_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1346134.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1613..2542
                     /gene="psaA"
                     /locus_tag="VXM85_001585"
     CDS             1613..2542
                     /gene="psaA"
                     /locus_tag="VXM85_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1346135.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKATTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2665..3156
                     /gene="tpx"
                     /locus_tag="VXM85_001586"
     CDS             2665..3156
                     /gene="tpx"
                     /locus_tag="VXM85_001586"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1346136.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3252..5960)
                     /locus_tag="VXM85_001587"
     CDS             complement(3252..5960)
                     /locus_tag="VXM85_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1346137.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NVFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     YKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5962..6663)
                     /locus_tag="VXM85_001588"
     CDS             complement(5962..6663)
                     /locus_tag="VXM85_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346138.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6913..9324)
                     /locus_tag="VXM85_001589"
     CDS             complement(6913..9324)
                     /locus_tag="VXM85_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1346139.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKISTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9377..10069)
                     /locus_tag="VXM85_001590"
     CDS             complement(9377..10069)
                     /locus_tag="VXM85_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1346140.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10604..10939)
                     /locus_tag="VXM85_001591"
     CDS             complement(10604..10939)
                     /locus_tag="VXM85_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346141.1"
                     /translation="MGGILLLICPFVLLGTAVNTAATTLNGGATAGAFSGVALLLNAL
                     KIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILAIIGGSLFLA
                     TLKKFKSEE"
BASE COUNT         3201 a         2515 c         2051 g         3188 t
ORIGIN      
        1 ggaatacaag tttatgatac gtatcgaaaa cctcagtgtc tcctacaaag aaacgttggc
       61 acttaaggat atttcactag tgctccatgg accaacaatt accggcatca ttggtccaaa
      121 cggcgctggg aaatcaacac tattaaaagg tatgctggga attatcccac atcaaggtca
      181 ggcatttctc gatgacaagg aagttaaaaa atccttacac cgaattgcct atgtcgaaca
      241 aaaaatcaat atcgactaca actttcccat caaggtcaag gaatgcgtct cgttaggact
      301 atttccctct attcctctct ttcgaagttt aaaggctaaa cattggaaga aagtgcaaga
      361 ggcccttgaa atcgtcggcc tagctgacta cgctgaacgt caaattagtc aactgtctgg
      421 aggtcaattc cagcgggtct tgattgccag atgtttggtg caggaagccg actatatcct
      481 cttggatgaa ccctttgctg ggattgactc tgtcagtgag gaaatcatca tgaatacgct
      541 gagagatttg aaaaaagctg ggaagacggt tctcatcgtt caccacgacc tcagcaagat
      601 tccccactac ttcgatcaag tcttacttgt caatcgagaa gtgattgcct ttggtccaac
      661 aaaagaaact tttaccgaaa ccaatctaaa agaagcttac ggtaatcaac tctttttcaa
      721 tggaggtgac ctatgattgc agaatttatc gatggattgc aaaaattcca tttcctacaa
      781 aatgccttga taacagctat tgtcgtcggg attgtagctg gagccgtggg atgtttcatt
      841 attctacgcg ggatgtcact catgggagat gccatttcac atgctgtctt accaggtgta
      901 gccctctcct tcatcttggg ccttgacttc tttatcggag ccattgtctt tggattgcta
      961 gctgccatca tcattaccta catcaaagga aactcgatta tcaaaagcga taccgccatc
     1021 ggcattacct tttcttcttt cttagccctc ggtatcatct tgattggtgt cgctaaaagt
     1081 tcaactgacc ttttccatat cctttttggt aatatcctgg ccgtccaaga tacggatatg
     1141 tttattacta tgggtgtggg ggcagccatc ctcttgttaa tctggatttt cttcaagcaa
     1201 ctcttgataa cttcctttga tgaactcttg gctaaagcca tgggaatgcc tgtcaatttc
     1261 tatcactacc ttctcatggt actcctgact ctcgtgtctg tgacagccat gcaaagtgtc
     1321 ggaactatcc tgattgtagc catgctgatt accccagctg caactgctta tctgtatgct
     1381 aatagtctga aaagcatgat tttcctttcc tcaaccttcg gagctactgc ttcagttttg
     1441 ggactcttta ttggctatag ttttaatgtt gcggcaggtt ctagtatcgt gcttacagct
     1501 gctagtttct ttctcattag cttctttatc gctcccaaac aacgatattt gaaactgaaa
     1561 aataaacatt tgttaaaata aggggcaaag ccctaataaa ttggaggatc taatgaaaaa
     1621 attaggtaca ttactcgttc tctttctttc tgcaatcatt cttgtagcat gtgctagcgg
     1681 aaaaaaagca acaacttctg gtcaaaaact aaaagttgtt gctacaaact caatcatcgc
     1741 tgatattact aaaaatattg ctggtgacaa aattgacctt catagtatcg ttccgattgg
     1801 gcaagaccca cacgaatacg aaccacttcc tgaagacgtt aagaaaactt ctgaggctga
     1861 tttgattttc tataacggta tcaaccttga aacaggtggc aatgcttggt ttacaaaatt
     1921 ggtagaaaat gccaagaaaa ctgaaaacaa agactacttc gcagtcagcg acggcgttga
     1981 tgttatctac cttgaaggtc aaaatgaaaa aggaaaagaa gacccacacg cttggcttaa
     2041 ccttgaaaac ggtattattt ttgctaaaaa tatcgccaaa caattgagcg ccaaagaccc
     2101 taacaataaa gaattctatg aaaaaaatct caaagaatat actgataagt tagacaaact
     2161 tgataaagaa agtaaggata aatttaataa gatccctgct gaaaagaaac tcattgtaac
     2221 cagcgaagga gcattcaaat acttctctaa agcctatggt gttccaagtg cctacatctg
     2281 ggaaatcaat actgaagaag aaggaactcc tgaacaaatc aagaccttgg ttgaaaaact
     2341 tcgccaaaca aaagttccat cactctttgt agaatcaagt gtggatgacc gtccaatgaa
     2401 aaccgtttct caagacacaa acatcccaat ctacgcacaa atctttactg actctatcgc
     2461 agaacaaggt aaagaaggcg acagctacta cagcatgatg aaatacaacc ttgacaagat
     2521 tgctgaagga ttggcaaaat aagcctctga aaaacgtcat tctcatatga gctggcgttt
     2581 tttctatgcc cacatttccg gtcaaatcat tggaaaattc tgactgtttc agatacaatg
     2641 gaagaaaaaa gattggagta tcctatggta acttttctcg gaaatcctgt gagctttaca
     2701 ggtaaacaac tacaagtcgg cgacaaggcg cttgattttt ctcttactac aacagatctt
     2761 tctaaaaaat ctctggctga ttttgatggc aagaaaaaag tcttgagtgt cgttccttct
     2821 atcgatacag gcatctgctc aactcaaaca cgtcgtttta atgaagaatt ggctggactg
     2881 gacaacacgg tcgtattgac tgtttcaatg gacctacctt ttgctcaaaa acgttggtgc
     2941 ggtgctgaag gccttgacaa tgccattatg ctttcagact actttgacca ttctttcggg
     3001 cacgattatg ccctcttgat caacgaatgg cacctattag cacgcgcagt ctttgtcctc
     3061 gatactgaca atacgattcg ctacgttgaa tacgtggata atatcaattc tgagccaaac
     3121 ttcgaagccg caattgcagc tgctaaagcc ctatagaaaa aatcctctgt cacaaagagt
     3181 tccctgctct ttgaaacgga ggatttttga atattagtaa atagaagttc aatcagctaa
     3241 aaataactac cttactctac agatttcagg gcttctaaca tatcaacctt tctcagataa
     3301 taattgacga agaaaccaag caaggtcaaa atgatgctta ctgctgccac tgggattaca
     3361 tagacttccc agcctacctg cggataaaag agaatagtcg caggcgaaat catttgaatc
     3421 aaaaattggt gtaaatagaa accagctatc agaccaagta cgattcccac aagggacagc
     3481 acaatcgtct cacggtaaat gtagagggtg acttcattat tatgaaaacc aagaaccttg
     3541 atagtggaga gttcacggat tctctcagct acgttgatat tggtcagatt gtaaaggatg
     3601 acaatagcta atagaaccga tacgatgacc aagatggtca tggtctgatt gagtgagcta
     3661 gcgatagagt cgaagagtcg aatggctgaa gcattttgga caacgctgga caccgcagat
     3721 tgattcataa gcaagcccgc ctgactttcg atactagttg cactggtatc ccttaatgag
     3781 accagataag tgttggcttg gggtagctgt ccgtaaagtt gctcatagct agcctgactc
     3841 atataaataa agtgaccaac gtagttctca gtaatagcag cgacctttag ttccttacct
     3901 tcaatttcta aagtctgccc aaccttgaca cctgccagct gggcgagttt agctgtaata
     3961 acgatgccat cttttaatgt cagctcctgc tgatgatgtt gaagatggat aaagggagtc
     4021 aaatcttcct tctctatcat cataagagta atgttttgaa gaccagcttt gcctttgaaa
     4081 tccttgtata gcgctttaga atagattttc tggtaggcta gtatctcctg ccctttcaac
     4141 acttctgcta gctctacctt gtcctgattg gtcgcactag gattttcaga gacaagcatc
     4201 tgatactgtt ggatttgttg aaactgttta gacggaactc ctgctacaga agattggatt
     4261 cccaaacctg caaagagcag agctacagaa cctgccacac caaagattgt catcaacatt
     4321 ctctgcttat aacgaaagat gttgcgggct gttaccttat gagtaaaact gagacgacgc
     4381 cagataaaac cgatacgctc caataagatt ttagctcctt tgacaggagg tttaggaagt
     4441 agaagctggg ctgcttcgtc atgaagttcc ctccaagcca ccagataggc tggtaacaca
     4501 ctcgccaaca agctcaagac aaaagctagt aagctatagg tccaatagaa ctgaatctgg
     4561 gtttctccca ccaccatgcc ttttgtaatg acacttgaaa ttacactggc tagcaaataa
     4621 tgaccaagta tactacctag agccgttccg acagtcccag ctactagtcc ataaaggaga
     4681 aacttggcga taatatcctt actacgataa cccaaggcct taaaaatccc tgcatgagtt
     4741 cgctcttcgt ctacaaagcg agtcatggtc gtaaaggtca ccatggctgc tacggcataa
     4801 agtaccacag gaaagatatt gcccactgct cgaatactgg atgaagcatt gctatacata
     4861 agatagccct gaccacctgg catggtctga cgattataga cctgatacct tggctccgcc
     4921 aaatcgtcca agacttgctg atgtttttct aatttttcct tttcttgggc tagattttgt
     4981 tcagcttgct ttagtttgtc ctcttccttg cccaattcct gcttggcttg ggtaagttga
     5041 gcactagcct gctctctctg aacttgagga aacaaggcta gttgactttc ttgagcctgt
     5101 atacgagctt gagcagctgc taaacgacgc ttgccttcct gcaaattagt ctgagccttg
     5161 tcaagggtct cttgcccctt gtctagagac tcttgtcctt cttttttcaa aagttgcaga
     5221 cgtaccttgc cattatctga taaaatttgt tcaagctctt cctgatgttg cttggatttt
     5281 tcttcataag ctgatgaaaa gacatttaaa cctgctaaat cttgatattt caaacgagct
     5341 atattgtaga ctttctgatc aaactgacta ggtaaaatca ccccataggc tgtcagagtc
     5401 ccacttccac ttcctgcgta gcccatatct cgctgggaga ggatttcagc cgaatccaca
     5461 aaaccagtaa tggtataagt atggtctttt aaagaggaat gaccctcttc tttttcttta
     5521 aaactaatct cctgtcccac gctgtattgg ccttgcaaat gagtggccaa agcgatttcc
     5581 ttgtctgact gaggaagtcg tccctttctt agctgaaagg ttgaaattcg ctctggtttg
     5641 gagtacagtc gaatggcatc ctgcccatta tccatagtca catctgtcaa atagccaaac
     5701 tcgacctctg cgccctccgt ctgttttagt tcttcttggt ctgcttgatc caagccatag
     5761 ttagacatga ctgccaaatc taaggtttga gcagttgtta aataagcatt agctgtcgcc
     5821 tccatgttgg gactggttac tttgaggcct actaaggcta gagatcccaa catcatcagg
     5881 atcaagatgg ataaaaaacg ccccttggag cctgtgaagg actgaattaa gtccttccaa
     5941 taagtttttc gcttgatcat gctagtactc caaactgtca atatcctgag gatgctggtt
     6001 gagcaccaca tccttgacac tggcatcgtg catttgaatc acgcgatcag caatgggcgc
     6061 caaagctcca ttatgagtca cgatgatcac cgtcgctccc ttttgacgag acatgtcttg
     6121 gagaattttc aaaacctgct tgcccgtctg ataatccaag gctccagtcg gttcatcaca
     6181 aaggagaatt ttaggatttt tggctaccgc gcgtgcaatg gagactcgct gttgctcccc
     6241 tccagaaagc tgggctggaa agttattgag acgatgagcc agacctacat ctgtcaagac
     6301 ctgatcagga ttcaaggcat ctgtcacaat ttcagaagcc agttccacat tttccttagc
     6361 tgtcagatta gaaactagat tataaaactg aaaaacaaac cccacatcat ttctacggta
     6421 attggtgcgc tggtgggaac tataatccgc aatattaaca ccatcaatcc agatttcccc
     6481 ttcatcattg gtatccattc ccccaagaag gttaagaact gttgacttgc ctgcacctga
     6541 agcaccaagg ataataacca gctccccctt ttcaatctca aaattcacat cacgattggc
     6601 cacaatctcc gtgtccccaa cctgataacg cttgtaacag tgtttcatct caatataagc
     6661 catcagatcc accctctatc aaacaaatta tactcctcga aaatctctct tcaaaccacg
     6721 tcagcttcca tctacaacct caaaacactg ttttgagcaa cctgcggcta gcttcctagt
     6781 ttgtgctctt tgattttcat tgagtattac ttcctattat cattataacg ctttttcaac
     6841 tagttggaaa ctgcctatat tcttaaatcc ttattaaaag gaagtttcaa attactgcct
     6901 tataacatct tcttaaaaat aaaattgaac aagatcacct tctgctgttg ccaccgaaat
     6961 acaatctttc cccatgcatt tcgcttttat tttttcttga ttcattttaa tcacactctt
     7021 ctcaatatct ggataagaaa ctcgcaaatc tcctccactc cttgataaaa tggtcaactg
     7081 taagagtttt ttatcttccc agctcatgct cacttcaaaa tgtccacgtg ccattaagcc
     7141 tgaaacagaa cctgttgacc aagcatcagg tagggcagct agaggtacca gataagctgc
     7201 atgagactgg agtaacattt ctgccatgcc actagtagca ccaaaattac catctatctg
     7261 aaaaggagga tggctacacc aaagatttgg caaggtggat atttttaact gctctgccaa
     7321 taatttatgg gctcgattgc catctcccaa acgcgcccag agattgatct tattagcctt
     7381 ggaccagcct gtgccgccat ctccacgatc attgaggcta gcacgtgccg cttcaatata
     7441 ctcttgtccc ttgtagctaa agagattgcc aggatagagt cccactagat gggaagcgtg
     7501 ccggtgctgg gcctccactt tctcattttg aaaatactgc tcttcctcct cataccactc
     7561 cctgattcga ccagattgag tgatttgcaa aggatttagt aaatcagact tctccttaac
     7621 ctcagtcaac aagtcctcat ccagtcccaa ttcctgagca gcctgaataa aatcatgaaa
     7681 taactgccaa atcagagatt ggtcataagt attgccaatc gaaatcggcc catgttctgg
     7741 ggaataagac ggagaagaca cccaacgctg cgcctgctga tccttatgta aaaaggcatt
     7801 ccaaaaacga accgtttccc tcaacatggg ataaattttc tctctgagat agtcttggtc
     7861 cctataaaat gaataggctt cataaacggt ttgcatcatc cacgcattgg cagctggtga
     7921 ccaaccccaa tagtaatccc aaccaggtgc cgtccaacca aagggagtcg cttgagtatg
     7981 aaccaaccaa ccattctcct cacctttctg agagacgatt cctgcatact ttacagccgc
     8041 tagacgacca tagacacgca aatcatctac atagttgatg actggaaaga ccgtctctag
     8101 gagattggta acataggctg gccaataatt catctgcaga ttgacattta agtgatagtc
     8161 cgaattccaa ggaggattgt cgaccgcatt ccagactccc tgtaggttag ctggtagagc
     8221 atctgggcag tctctggacg aactaatcaa taaataccgt ccatactgga agaacagctc
     8281 ctccaaagcc tgcccttctt gtggcttata attttttaac aaatcatctg tagtggatgc
     8341 gtcaacatca gcttccaaat ccaattgaac acgctggaat aaggcttggt agtcctcgat
     8401 atgccttgat ttcaattggg tatagccctt ttctttagct gtgtccacca agtctatcac
     8461 ctgttgctct aaatctagtt tcttgcgata attgctagca ggattttggg caaaatccgt
     8521 cttagcggcc aagaagagat tggcataact ggctcctgat atctgaaccc tatctgacca
     8581 aactctaata tctccatccg tttcccaagc tagataacta gcaaaccgca gatcattatc
     8641 cttaactctt cccttcatca agatatgaga atcagtaata tccaacttac actccttgta
     8701 atcagatttt tcctgctcat actttccatc agaagccaaa tcacaggtca aggatagttc
     8761 tatagtaaaa tctagagttt ccaacccttc cttagtaaaa cattgaatca agagatcatc
     8821 tggaaaactc gcaaaagctt tacgttcaaa tcgcgttccc ttatagacat aagaagtcgt
     8881 cgcaagtgcc ttactaatat tcagctgtct ctgatagtcc gtcacctgag acaaagtcgt
     8941 accttgctgg ctgaactcaa tgtgaatatc cccaaaagac agataggtcc catattgact
     9001 cgtttttggc ccaattaggt gctgctcagc cagttcctta gccagattgt aatctctctt
     9061 ctccaaagcc tgccgaatct cagctaaaaa aacatactga tcctgaagat ttccaccctg
     9121 ataatctgaa ctatcaggaa gtggacctcc agaccagaga cttttttcat taaattgaat
     9181 ccgttcagcc cctataaggc caaatacttt tgctcctaaa gaaccattac ctatcggtaa
     9241 agcttcttct tcccaaccca tgtaagtggt tgaagctggt tgcttgtagg ccagtacata
     9301 atcttgtttt ttattcctta tcatacttcc atactaacat aaaaagccta gaaatcaatc
     9361 ctaggcttac aattttttat tttccaaggt agtattcaga aacaacgttc aatttttcgt
     9421 cgaattcgaa taccaatggt gggaagttag ggatttccac gtccatgatc tcatcatctg
     9481 acaaaccttt gatgtgtttt acaagggcac ggattgagtt accgtgagct cctacgaata
     9541 cgtttttacc atctttaaga gctggagcga ttttatcttc ccagaatgga agagcacgtt
     9601 ccaaagtcac tttcaagttt tcagcatctg gaatgactga gtcgtcaagt gaagcataac
     9661 gacggtctgt gtgagctgag tgctcatcat cacggtccat atttggaggc aatacatcgt
     9721 atgaacgacg ccagatgtga acttgctcat caccaaattg ttcagcagct tcagctttgt
     9781 ttttaccagt caaaccaccg tagtgacgtt cgttcaagcg ccatgatttt tcaactggaa
     9841 cccacaattg gtcagaagct tcaagagcca agttagttgt tttgatagca cgtttcaata
     9901 ctgaagtgta agcttggtca aattcgatac cagcttcttt gatcaattta ccagcgtcaa
     9961 tcgcttgttg tgtacctttt tcagacaaat caacatcagc ccaaccagtg aaaaggttag
    10021 ctttgttcca ttcagactca ccgtggcgag caaaaaccaa ttttaccatt agatggattc
    10081 tcctttaaat tttccgaggt ttcccctcgt ttatctattc tattttacac aatttttcac
    10141 aaaaaagcta gtcaaaacca aaaaggaaag gactgtttga caatgtttgg caatcacatt
    10201 gtttttggtt gtttctattc tatcttcatt atttaacctt tttttctgcg catcgaataa
    10261 tttttgagtt gactctttta gtttagtaaa cgagtcatta ttttccatag ttgtgccttt
    10321 gaaaccttcc tcagctttaa taaaataatc atcaaaaata tttttataaa gatctatata
    10381 atctgcataa gtgcgtattg attcaatcgc atcgtctgtt acaaccggtg tatcgaaagt
    10441 gattgtttct tttgaactat tggatgttgc tactgtcttc tcatcattac ttgttgaagt
    10501 attctttgaa ctttatgttc cacatgcaac tagaagagaa agagataata gagctactcc
    10561 tataccgata aacttttttg ttcttttcat gctaaaatac cttttattct tctgatttga
    10621 atttcttcaa tgttgcaagg aatagagatc ctccgataat agcaagaatc cccccaaccc
    10681 atcctaagaa tggaatgaga ctaacttcac cagaaacaat cattagtaca gacggagctg
    10741 cacctacatg cttatctcct ttatagtaaa caatagcaat gataccaaga acaagatttg
    10801 caatcttcaa ggcattcaag agtaaggcaa cacctgaaaa agcccctgca gtagctcctc
    10861 cattaagagt tgtagcagct gtatttaccg ctgttcccaa caagacaaaa ggacaaataa
    10921 gaagtaaaat tcctcccaca agaccgacga tacca
//
LOCUS       DAVLRT010000062        10944 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLRT010000062 DAVLRT010000000
VERSION     DAVLRT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.062"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(15..650)
                     /locus_tag="VXM85_001592"
     CDS             complement(15..650)
                     /locus_tag="VXM85_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1346142.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQADQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(652..1479)
                     /locus_tag="VXM85_001593"
     CDS             complement(652..1479)
                     /locus_tag="VXM85_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1346143.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1501..1837)
                     /locus_tag="VXM85_001594"
                     /pseudo
     CDS             complement(1501..1837)
                     /locus_tag="VXM85_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(1838..2554)
                     /locus_tag="VXM85_001595"
     CDS             complement(1838..2554)
                     /locus_tag="VXM85_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV1346144.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRVQAPLVIKEVNGIKVALLAYSYGFNGIEQYISKEDYNRYLSD
                     LNEDKMKAEIERAEKEADITIIMLQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSEMVEKDGDKKLIIY"
     gene            3067..3678
                     /locus_tag="VXM85_001596"
     CDS             3067..3678
                     /locus_tag="VXM85_001596"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1346145.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3723..4451
                     /gene="rlmB"
                     /locus_tag="VXM85_001597"
     CDS             3723..4451
                     /gene="rlmB"
                     /locus_tag="VXM85_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1346146.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4490..5401)
                     /gene="trxB"
                     /locus_tag="VXM85_001598"
     CDS             complement(4490..5401)
                     /gene="trxB"
                     /locus_tag="VXM85_001598"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1346147.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVRKIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5467..5691)
                     /locus_tag="VXM85_001599"
     CDS             complement(5467..5691)
                     /locus_tag="VXM85_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1346148.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5769..6512)
                     /locus_tag="VXM85_001600"
     CDS             complement(5769..6512)
                     /locus_tag="VXM85_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1346149.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(6512..7312)
                     /locus_tag="VXM85_001601"
     CDS             complement(6512..7312)
                     /locus_tag="VXM85_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1346150.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNLGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7470..7826)
                     /locus_tag="VXM85_001602"
     CDS             complement(7470..7826)
                     /locus_tag="VXM85_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1346151.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7839..8351)
                     /locus_tag="VXM85_001603"
     CDS             complement(7839..8351)
                     /locus_tag="VXM85_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1346152.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGEPQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNK"
     gene            complement(8351..8845)
                     /locus_tag="VXM85_001604"
     CDS             complement(8351..8845)
                     /locus_tag="VXM85_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1346153.1"
                     /translation="MEIRLAFPNEVDAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            8980..9426
                     /locus_tag="VXM85_001605"
     CDS             8980..9426
                     /locus_tag="VXM85_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1346154.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTIKLYHQTLDLIDHTQEEVIQ"
     gene            9423..10070
                     /locus_tag="VXM85_001606"
     CDS             9423..10070
                     /locus_tag="VXM85_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1346155.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIVYYM"
     gene            complement(10422..>10944)
                     /locus_tag="VXM85_001607"
     CDS             complement(10422..>10944)
                     /locus_tag="VXM85_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346156.1"
                     /translation="INTGFWNLLSNPSYLKSIQTSMSMGVTLISGFILYTQNMYSFMN
                     TANGVNEARASFIAYMSAPIILIITSSISLTILSSNKDIEDIKQLKTLGVSRLQLFKI
                     RIGEAIIHSVLILLVSVIFNLIILILVSIIGQFLGRSLVDISGFWQPSLIVISLLVIF
                     YSITKGFYLFQDR"
BASE COUNT         3280 a         2493 c         1886 g         3285 t
ORIGIN      
        1 cattatttag ctatttagta aagatagtct ttcaaggctt ttgacaaagc ctgataacca
       61 gcaatactga ggtgcagtcc atcagttgta tattcttttt tgagttggtc tgcttggtct
      121 gtcaaacaat caaatactgg cacaaattcc acctgcatat aggcagatgc aagctcttga
      181 taggcttgat tccagttctg aattttttca ttcgagcgga tatagactgc ctgctggtac
      241 tcctctccct cattgacagg caaaatggaa agcaatttaa tctctgtcaa tggataatcg
      301 cgagcaacgg attgaatgat agcttcgaga ttattgagag cctcattcac aggaacatcc
      361 tttccgatat catttgtccc aatcagaaga aaaattttat ctactgctcc accatataga
      421 tgagcatcaa ggttctctag taacagtcct gtctgatagc cacgaattcc tcgattgaca
      481 atcgtctttg aagtcccaaa tagctcctgt agaggataat actcgacaat ggaatcccca
      541 ataaaaagaa tgtttggttc tacaacagaa atgtgattta ggtgacgata cttagtttga
      601 attttttctt gttcctttag gagccaattt tctaataact gtactgccac ttcattctcc
      661 tttttctaac cagttttcca aaacttgata aactcctcct tggctgttgg ctggtgctag
      721 agcagtcgcc acagctttgg ctttctcatc agcattttcc atcgcatagg caattccagc
      781 catttcaagc atttcaacat cattttcact atcaccaaaa gccatgattt cttgggattt
      841 caagtcccag cgcttgagta attcctccaa gccccatgct ttatgaatcc cagcttggag
      901 gatatcaatg caaccatagc cactggatac agctcggaca cggccatcaa agagagcatt
      961 gatttcttcc aaaaccgaac tcaaacgttc ctcaccaaca accatgctca tcttgagcac
     1021 accaccaaag aggtcagatg ttaattcatc cacaaattgc atccgttggt agaatttttc
     1081 aatcatttct ggagtcataa aactttcaag atctgtaaaa atcgtacctt ccttgacaaa
     1141 atcacccttc atccccgtta caacaaactg gtcctgacac gctcgaccct tgaaatgagc
     1201 caaagccttg ttgacaatgg cgtcatccca tgtctgagcc tgaatcaatt cattgttttc
     1261 aaaaatacga gcgccattag caacaaccag aaccactcga tccaccaagg gactcagtag
     1321 ttgtctcatg cggtgaattt cattgcccgt cgcaatgaca aaacgaatgc ccctttgatc
     1381 caactgatct aaaatctttt ccaatcgtgg gagatcaagc tgacccctag catccagcaa
     1441 ggtcccatcc atatctgtcg caattacctt aatcatcatt tactttcctc tggattcaag
     1501 ctaataccac ttcaatccca catgttcatt catttcttta taggctgtat caattcgttc
     1561 cttagtcgct tcatctaact ggtcacgatg actgccaccc ctctataaaa tcttccaaaa
     1621 tataagtttg gtaatgatac aagggatatc cttctggtga aaaggttccc tttggtgttc
     1681 gattgaccca agtaggatga gctttagctg ttccgatagt tgtttttcca tccttcttct
     1741 tgatggtgac atccatgaga acaccacgtt cagtccactt agcattctct tcatctccca
     1801 tagattcaat tcgttgattg gaaatgaagt tccccattta atagataatg agtttcttat
     1861 ctccatcttt ttcaaccatt tcagatggtt caacaacgtg aggatgccct ccaaagataa
     1921 tatccgctcc ccaatcgatc atcttgtgat aaagagcttt ttgttcttca gttggttcca
     1981 atcgatactc aacacccatc tgaagcatga taatggtgat atctgcttcc ttctctgccc
     2041 gttcaatttc agccttcatc ttatcttcgt ttaaatctga aagataacga ttatagtctt
     2101 ccttagaaat atactgctca attccattga aaccatagga ataggctaac aatgcaacct
     2161 tgataccatt cacttcctta atgaccagcg gagcctgaac acgtggttcg tgcgtataaa
     2221 ctccgattgg agtgattcca gctttctcaa taatatcggc cgttgaaata actccctcaa
     2281 tttgcgaatc caaaatatga ttatgagcta aatccagcac atgataacct gcctccttaa
     2341 tagcatccat aacttcaaca ggagcattaa agagaagata acccgctaaa taatgatcct
     2401 tattaatggt tccttcaaaa tcaccaatag ctaaatctgc ttgcttgagc caaggagtca
     2461 catactcaaa attttcatga aagtcatagg taccgtcttc ttttttagct gaaaagaaaa
     2521 gtccatcgtg gtagagcaaa tccccgtggg ccataattct ggcagttttt tcctcttcct
     2581 actcctgaga cttttgctta gtcccctctt gagaaatagt atctcgtttt tgactagtca
     2641 agggattcct tggaagcttt caaacaacaa gaccaagccc attgataaac caactgctag
     2701 caagagtatc gccacaaatc ccttattgct ccacttgcga taactcctaa aaagtttacc
     2761 aagcccttca taaaacgaaa agctaaacca ccctgatttc gattttgtct tctttgtatc
     2821 ttcattctcc ctactttctt atgcaagcct tttcttttta ttatatcaca gataagtatt
     2881 tctttcacaa ttgaattgaa cttcctatct attttctata aatcctaaat gccataatgc
     2941 tttcaattct tgtcattttg tgatatcatg tagaggaaat gaactaatcc acagtggctt
     3001 attccaagta taccacttgg gctttggcag tagctaactg cactaaacat aatataagga
     3061 gagaaaatgt ctgcaataga acgtattaca aaagctgctc acttaattga tatgaacgat
     3121 attatccgtg aagggaatcc tactctacgc acggttgctg aggaagtcac tttcccccta
     3181 tctgaccagg aaatcatcct aggcgaaaag atgatgcaat tccttaaaca ttcccaagat
     3241 cctgtcatgg ctgaaaaaat gggactccgc ggtggtgttg gactggctgc tccccagtta
     3301 gatatctcaa aacgcattat cgctgttttg gtacctaata ttgttgaaga aggcgaaact
     3361 ccacaggaag cctacgattt ggaagccatt atgtacaatc caaaaatcgt ctctcactct
     3421 gttcaagatg ctgctcttgg cgaaggagaa ggttgcctgt ctgttgaccg taacgtgcct
     3481 ggctatgttg ttcgccatgc ccgcgttact gttgactact ttgacaaaga tggagaaaaa
     3541 caccgtatca aactcaaagg ctacaactcc attgttgttc agcatgaaat tgaccacatt
     3601 aacggtatca tgttttacga tcgcatcaat gaaaaagacc catttgcagt taaagatggt
     3661 ttactgattc ttgaataaag aaaatcccgt tgcaagacgg ggttttgtgt tataatagag
     3721 gcatgaaaac aaatgatatt gtctatggtg tccacgccgt taccgaagcc ctccttgcaa
     3781 atacaggaaa caaactctac ctccaagaag atctccgagg taagaatgtt gagaaagtca
     3841 aggaactagc tacagaaaag aaggtgtcca tttcttggac atcaaaaaaa tctctctctg
     3901 agatgactga aggtgctgtt catcaaggtt ttgttctacg agtgtctgaa tttgcctata
     3961 gcgagctaga ttacatcctt gcaaaaacac gccaagaaga aaatccactt ctattgattc
     4021 tagatggtct aactgatccc cataatctgg gttctatctt gcgaacagcc gatgcgacca
     4081 atgtttcagg tgtcatcatt cccaagcacc gtgctgtcgg agtaactcct gtcgttgcca
     4141 aaacagccac aggtgctatt gaacacgttc caattgcccg agtgacaaac ctcagtcaaa
     4201 ccttagataa actcaaggat gaaggcttct ggacctttgg aacggatatg aacggtactc
     4261 cttgccacaa gtggaataca aaagggaaaa tcgccctcat catcggaaat gaaggaaaag
     4321 gcatctctag caacatcaaa aaacaggtcg atgaaatgat taccattccg atgaatggac
     4381 atgttcaaag cctcaatgcc agtgttgctg cagccattct catgtacgaa gttttccgaa
     4441 atagactata aaaaagtttc cagtcatctg attggaaact tttttatgat taactatgtt
     4501 ctgtgataaa cttgtaggct tcttgaccag cgatagctcc atctccaact gctgttgtta
     4561 cttggcgaag gtctttcaag cgaacatctc caactgcaaa gataccgtcg actgcagttt
     4621 tcatgtggtt atctgtcaca atccatcctg cctgatcttg gatattcaat tctttaacaa
     4681 aatcgctaag agggtccaat ccaacataga taaagacacc accgaaggct tgttctgtca
     4741 cttgacctgt tttcacattt tcaaatacga ctgattctac tcggttttca cccttgattt
     4801 tccttactac agaatcccag ataaagctga ttttctcatt ggcaaaggcg cgatcttgta
     4861 aaaccttttg ggcacgaagt tggtcacgac ggtgaacaat ggtaacagtc ttagcaaaac
     4921 gagtcaagaa gagggcttct tcaacagctg aatctccacc accaactacc aataaatctt
     4981 ggtcacggaa gaaagcacca tcacacacag cacagtaaga aacaccacga ctgttcagtt
     5041 cttcttctcc aggcactccc aaaggacggt gtttagaacc agttgctacg ataactgtac
     5101 gtgtttcata tgtttggtca tcagtcatca ctttcttaaa atcaccatgg tcttcgacat
     5161 tttcaacata accataaatg tgctccacac caagattttc aagtggttca aacatctttt
     5221 cagccaattc aggtccacta atattagcgt atcctgggta attttcgata tcagatgtat
     5281 tattcatctg accacctggc agaccacctt caatcaaagc tacttttaga ttgcttcgag
     5341 cagcatacaa ggccgcagtc atccctgcag gtccagcacc gataataata gtatcgtaca
     5401 tatagattcc ttctttcttg ttgtaactat ctttattcta actctttctt gtcaatcacg
     5461 caaggattat gccttgaaaa caagaattaa actcataacc gcaaaggata atactgggac
     5521 aaccaagact ccaatcataa tcatatcata gagatgggct tggcgagcct tggctgtctt
     5581 atcaactccc gacatggctc tcagaccaat ccaaatccct aaaaaaatca gaacaaggat
     5641 ggtggtcaat atcaaactct cgaaatataa agaaaatagt tgcagtagca tgatttctct
     5701 catttctatc ttttttaaag agtaaactca gctagtccaa ctaactgagt tttcctttat
     5761 ctattatatc aaatataagt ccgtttgtaa ctagcgaaga attcttttgt ccgctcttct
     5821 ttaggggtgt ggataatctc atccggagtt ccagactcga tgattttccc cttatctaag
     5881 aagagaatct tatccgcaac ttgggctaca aaggacatgt catgactgac caaaatcatg
     5941 gtctgacctg acttagcagc atctgcaata gacttttcta cttcaccgac caattctggg
     6001 tcaagggctg aagttggttc gtctaagagc aaaacatctg gtttcatagc aagcgcacgc
     6061 gctagggcaa cccgttgctt ctgtccacct gataaatggc gaggataatg gttttcacgg
     6121 tccgaaagcc caaccttagc caactcttcc ttggcaatct tagtcgcttc ttggtcagat
     6181 aatttcttga caacaaccaa gccttctttc acattatcaa gtgctgttcg gcgttcaaac
     6241 aaattaaact gttggaaaac catagacaac ttacggcgta gggcaaggat ttcttcttga
     6301 gtaattttag aaaaatcaac tgaaaaacca tcaatctgaa tagagccact gtcaggtgtt
     6361 tcaagataat tgagactgcg aagaaaggtt gattttccag ctcctgaaga accaatcaag
     6421 gctacaactt cccctttttg aatatccaag tccagatgat ccaagacagt ctgtcctgaa
     6481 aaggatttgc ttaaattcga aatcttaatc attaacgaag gtctcctttc acatctgttt
     6541 gcactgtatc aggtgcagaa atagccattt ttctctcgat gaaacgaccg aggctttcaa
     6601 ttccgatatt gactacccaa taaacaaggg caacggagat gaagcgttca aaatagcgat
     6661 aatcagctcc acctagaatc tgagcttggg caaagacttc cacaacaccc gcactaaaag
     6721 ctagagatgt tcccttggtc aaaccgatga gggaattaat caaggttgga gtagctacca
     6781 ccgctgcatt aggaataatc actcgtcgat aaacttgcgc tcgggtcata cccagactgc
     6841 gtgccgcctc aatctcacca agattaactg agagaatggc tgcacgaatg gtttcactag
     6901 cataagctgc ctcattaaag gcaaaggcga caatcgcaaa agctgcagct ggaatcgcat
     6961 tgatattgag accagttccc cattgctgat tgagggcttt caaagccaaa gggattccgt
     7021 agtaggtcaa catgagttgc accaaaatcg gtgtcccttt taagaaacta acaaagaagg
     7081 cctgcaaggg atataaaatc ttgacacgat tgatcttcac aatggcaaaa agaagcgcca
     7141 aaaccaagcc aaaaagggca ccaccaattg tcaacataat tgttgttgga agttgttgga
     7201 caattctagg gattccatca aagaccgaac gtaggctaaa cagcttgcca tccggaatca
     7261 attgcatcaa gttttggtac caatctgatg ctaaaatcgt tgtaacattc ataaaaacat
     7321 cctcttctga taatgattca ttctaccatg cttctgccgt caatgaaatt atttgctacg
     7381 gtcagataca gactttgtct acctactagt ttatcatact ttgaaacagg gaagttatat
     7441 attttatcta tcaaagagat agataaaaac tatttcagtc ccagttcttc ataagatttt
     7501 cgataaccga tttgcttaac agttccattt tctactaaaa tgggccgttt taacaacata
     7561 ccgtcacttg ctaacaactc agccgcttct tggtttgaca aacttcctac cttatctttt
     7621 agccctaatt cacggtattt gattccactg gtgttgaaaa attgcttcaa ttcaaatcct
     7681 gaggtttcta gccaattcaa aatgacttct tggctaggtg tttcttccac gatatggacg
     7741 gctttatagt ccacacctaa ttgatttaat tcttgttttg cttttttaca agttgaacat
     7801 ttggggtatt cgataaattc taacatgtgc ttctccttct atttgtttct atctttaaaa
     7861 tctactcctt catgctccaa gagccaagct ttcttttcca ctcctgcagc atagcctgtc
     7921 agacgcttgc ctgctcccaa cacacgatga caaggtacta ggatagacca aggattgcgt
     7981 cccactgctc caccaattgc ttgagcagaa gccacttgca ggtcttgagc aatttgtcca
     8041 taggtcactg tctgaccata aggaattccc tgtaaatagg accaaactct cttttcaaaa
     8101 ttcgttccga ttggcgccaa gagcaagttg gataaatcct gaggctcgcc tttaaagtaa
     8161 tcatctaagc aagcaataac tgggtctaaa ataggatgac taacaacttc ttctatcgtt
     8221 tcatctccta gtcccctctc aaaatgcttc tgctcctgaa cccaaattcc atacaaataa
     8281 tggtcatcag ctacaagtga tagaatacca attggtgagg agtagaggat ttttacatac
     8341 ttcttttgca ttatttcttt aatttttgat aggccaagcg ttcgccatct actggcacct
     8401 taccaacttg tttaaaacca agtttttcaa aaatatgttg cataaccttg ttttcagcat
     8461 gcgtatctga gcgaaaatca agataatcaa aaccttcaat caagccctct aagaaggttt
     8521 gagcaactcc ttttccctgc acatctgctg ccacagcaat acggtgaaag actagatact
     8581 ctgactctcc agcttgccag tttccctcat aaatagcttc ataggcctcc tctggactct
     8641 tggtcacagc agcataggct agcagttctc cctcttccaa ggctacgtag gcttgccctg
     8701 agatgatatc atcgataata acatctgcat ttggatagcc attttgccac tggttactac
     8761 cagcatctgc taaacatttt ttggcatcct ctatcacctg catgatggca tctacttcat
     8821 ttggaaaagc taaacgaatt tccatctttt ttcctcattt ctgactagtt tctcccatca
     8881 tagcataatt taagtatttc tacaagcagt taaaacttga ctttgtgttt ttgttatttt
     8941 ataatctagt tatcaaaact atataaaaag gagttgaaaa tgaaaactac cttttcctac
     9001 ccaaaatggg cagaaattcc aaacattgac ctctatctgg accaggtttt gctctatgtc
     9061 aatcaggtct gcgcccctat ctctcctaat aaagacaagg gcctaacagc atctatggtc
     9121 aacaattatg tgaaaaatgg ttacctgaca aagcctgaca aaaaaaaata ccaacgccaa
     9181 cagattgccc gtttgattgc tatcacaacc ctcaagtctg tattttctat tcaagaaata
     9241 gcccagacac ttaatactct acaaactcaa gcaagttcag accaactcta cgatgctttt
     9301 gtggactaca tgaaccaagg aattgaccca gctaacccta ttatccaaac cagctgccaa
     9361 accattaaac tctatcatca aactctagac ttaatcgatc atactcaaga ggaggtaatc
     9421 caatgaacac tagtcttaaa ctcagcaaac aactcagttt tggagaggag attgctaata
     9481 gcgtgaccca tgctgtaggt gcagtcatca tgcttatctt gctgcctatt tcatccatct
     9541 atagttatga agcacacgga tttttatcct ctatcggcgt ttccattttc gtcatcagtc
     9601 tctttctcat gttcctatca tccaccattt atcactctat ggcctatggt tcgacccaca
     9661 aatatgtttt gcgaatcatt gaccattcta tgatttacgt tgccattgcc ggctcataca
     9721 cgcccgttgt cttgaccttg atgaataact ggtttggcta tctgattatt gtcatccaat
     9781 ggggaacgac catctttggt attctctata aaatctttgc taaaaaggtc aatgagaaat
     9841 ttagccttgc tctttacctg attatgggct ggttggttct ggctatcatt cctgccatta
     9901 tcagtcaaac gacacccgtt ttctggagtc tcatggtaac tggcggactc tgttatacag
     9961 ttggagctgg attttatgcc aagaaaaaac cttatttcca catgatttgg catctcttta
    10021 tcctagctgc gtccgtactt caatacatcg ctattgttta ttacatgtaa aactctcagt
    10081 aggcagagcc tgcttagagt tttacaaacg aatttcccaa agaggaagca atatgaaagc
    10141 agtcattgct tgtgttcata aaattctcag aatcatttac aaactactct ccaccaagta
    10201 gacttatcaa aaagaaaagg cgctaggact gaagaaacag ttctaacgcc caaattaaaa
    10261 aaatttcaac tacagtatag cacaggaaat gattttttgc gcttttttac tgttttttca
    10321 aataaaaaaa gacaaagttt tatccttgtc ttttagtttt agtattgctt tggtttcaat
    10381 gtcaataagt gtgttctttt tggttcagta actattccaa tttatctatc ttgaaataaa
    10441 taaaatccct ttgtaatact ataaaaaata actaacaaag aaataactat aagactcggt
    10501 tgccagaaac ctgatatatc tactaaacta cgacctaaaa attggccaat aatgctaact
    10561 aaaattaaaa taattaaatt gaaaattact gacactaata aaattaacac cgaatgtatt
    10621 attgcctcgc ctatacgtat tttaaaaagt tgtaatcttg acactcccag tgtctttaac
    10681 tgtttaatgt cctcaatatc tttattagaa gataaaattg taagagaaat agaactagta
    10741 ataatcagaa taattggtgc agacatataa gcaataaagg aagctcttgc ttcgtttact
    10801 ccgtttgctg tattcatgaa tgaatacata ttttgggtgt aaagaataaa cccagaaatg
    10861 agagttactc ccatgctcat tgaagtttgt atacttttca aatagctagg attggatagt
    10921 agattccaaa agcctgtatt aatt
//
LOCUS       DAVLRT010000063        10724 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLRT010000063 DAVLRT010000000
VERSION     DAVLRT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.063"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(83..1507)
                     /locus_tag="VXM85_001608"
     CDS             complement(83..1507)
                     /locus_tag="VXM85_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1346157.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVTWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2052..4331)
                     /gene="pulA"
                     /locus_tag="VXM85_001609"
     CDS             complement(2052..4331)
                     /gene="pulA"
                     /locus_tag="VXM85_001609"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1346158.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4443..6401)
                     /gene="ligA"
                     /locus_tag="VXM85_001610"
     CDS             complement(4443..6401)
                     /gene="ligA"
                     /locus_tag="VXM85_001610"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1346159.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6494..7672)
                     /locus_tag="VXM85_001611"
     CDS             complement(6494..7672)
                     /locus_tag="VXM85_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1346160.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7752..8615)
                     /locus_tag="VXM85_001612"
     CDS             complement(7752..8615)
                     /locus_tag="VXM85_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1346161.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISSHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8618..10519)
                     /locus_tag="VXM85_001613"
     CDS             complement(8618..10519)
                     /locus_tag="VXM85_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1346162.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3211 a         2368 c         1909 g         3236 t
ORIGIN      
        1 aaaagttcgt attttttcta taattattat tattttatag caaaaaaata aaaccaggaa
       61 aacaatttcc tgattttaca cgttatttca catcaaaaac aatggatttg acatttgtca
      121 tcgcttcaat gctatattta attccttgca ctccagctcc agaacctttg acaccaagga
      181 atgggaaatt atctggacca cgctgggttt tattattaat gtggactgta cctacttcaa
      241 gtttttcagc aatttcaaag gcttttttga aatcatttgt aaagactgat gattgaaggc
      301 cgaattcaga ttcgttggca aaggcaatag cttcctctac actagccaca cgaatgattg
      361 gtaaaacagg accaaatggc tcttcccatg tcactttcat atcttttgta acttggtcaa
      421 aaagcactgg ccagaggaga ttgccctcac gtttgattgg tgtaagagcc tgagcctctt
      481 tttcttgtgc atcctcaatc aagccccaaa tgaagtcggc tgaagcattg tcaataacag
      541 gtgtaatatc agcattgtca aatggatcac caactgttaa tttagaaact tcttcctgaa
      601 gcaaagtagc taatttatct gctacacttt cgagaacaat gacacgttta atggccgtgc
      661 aacgttgtcc tgagtagcta aaggctcccg caacaatttg cttggcagca tgttccaaat
      721 ctgcatcttc tagtacaaga gctgcatctt tcccaccaag ttccaacatg ataggacgca
      781 taccagctaa acgaccaata cgttctccaa taggagttga acctgtaaag ttgatgaagt
      841 tgacttcttt gtgctcaatg atataatccc caatttctga accacgacct gtaatggtgt
      901 tgaaaacacc tgccggaatc cctgcttctt caaatgcttt agccaacaag agtccagaaa
      961 tggaaccttg tgttggtggc ttcaacatga ccacattccc tgcaatcaag gcaggtgcaa
     1021 ttttagaagc agataaatta actggataat taaagggagc aatcgctagc acgataccaa
     1081 ctggttcacg acggacaaca gccagtttgt ttttacttgc tgcctcaaaa ccaccacctt
     1141 ccattgcttg tccagtgata cggagacctt cctcagcagc ataacgaatc aagtctgctg
     1201 tacgcactac ttctccaatt gctgctttaa tcccttttgc tacttctttg gcaaggatag
     1261 taccaatttt ttccttatcg cgttctaaaa tagctgctgt tttatgcaaa taagccgcac
     1321 gttcaattgc tgataaagct cgccatgctg gcagggctgc acgcgcagct tgcatagcct
     1381 catcagcttc agtctgagtc atggctggaa ctgtacccaa ttcttcttga ttgattggtg
     1441 aataaatcgt aatttcttgt tcagatgatt tccattttcc atttactaaa ttctgatatc
     1501 ttgtcaaatt cttgtcctcc tgaaaatgat taagatatat tttaccaaat tttcagaaaa
     1561 tcacaaccct ttttgcaaaa tttttgagat tattttcaca atcttgattt ttcaaagtat
     1621 actcaataaa aattaaaaaa atccactacg tcaaggcgag gctaatgtgg tttgaagaaa
     1681 ttttcgaaga gcatgaatga gtatcatcta tagtaaaata aaaaaactga acaatttggt
     1741 tggggacagc caaaccaatt tctcacaatg tttcagaaac aagggtgtgc tattccaatt
     1801 tcagcctact ataactgtca tagattgctg aaacaaagtc taggtaaaag tcttcataat
     1861 aaaaagacct cctatcaagt gttcaaaaac tttgatagga ggtcttgttt tgtgaaaata
     1921 tttatcaaat tttctataca agtgagctgt tagccaggtt ctttctattc tttcaatttc
     1981 aatgaatgga ttttttacta atactcataa ctgggaattt gtctgtgtaa aaatagcgag
     2041 atagatggta tttataaaac actcaagaca gctagactaa tatcatttaa aacattatct
     2101 tcttttgagc gactgttggt taccaacata gctaaatttc ctgcattttc aaattgatag
     2161 ggttctgatt tagcattcac aactaccaag aggtgttctt tgccgtgaac ttcatagata
     2221 aggcagccgc tatgttcaat cgcagaatgc acaaagacat gatggtaaat ttcatcatag
     2281 ctagagtaag aaaaggcacc agtttttgtc ttcaatcgga tgacttgacg gataaactca
     2341 atactgtctt gacgctcatt aatcaagttc cagttcactt ggttcacact gtcaggagca
     2401 ttatagctat tcatcgcacg ctctctatca tcatgggtca actcaccatt ttcaccagtc
     2461 gcaaccagtt tggtacgacc aaattcttga ccgatttcca taaaggccat cccctgcatg
     2521 agcagattca tggctgtggc agtttcgacc ttgcgcatga tttgctctga actttggtct
     2581 ggatgaaggg ttgccaataa atcgtgaaga ttgtaattgt catgggcttc tacatagtta
     2641 agcacctgat ttggatgtgt atagcttcct aattcacgac ttcctaggat tgctttagct
     2701 agaattggct ctgtcgcagc accactgaca aaacctgact tgatagcacc ataaacttct
     2761 ccccctttga cagcatcgcg ctgattgtca ttaaagaaac caatatttgg catctggtag
     2821 gcattgtcct tcttggcctt atcataaggg gcaagacctg ttcccatatc ccatccttct
     2881 ccatagagga taatgttgga gtcgatttca tccaagcttt gacgaatcat ctgcatggtc
     2941 ttgacatcat gaatccccat caagtcaaaa cggaagccgt caatattata ttcctgcacc
     3001 cagtatagaa gagaatcaat catatacttg cgaaacattt cgtgttcact ggctgtttca
     3061 tttccaacac ccgttccatt ctggaaggta ccatctggat tcatacgata atagtaatca
     3121 gggactgttg tttggaatgg tgcatcaaca actgagaagg tatggttata gactacatcc
     3181 ataatgactc caatacccgc atcgtgataa gcttgaacca tcaccttcaa atcacgaatg
     3241 acctgagctg gatcatctgg attagttgaa aaactagttt ctggcgcgtt atagttttgt
     3301 ggatcataac cccagttgta ggttacattt ccatcctcat cgtattcttt atgacggtct
     3361 gcaattggtt gcaattgaac ataattgtag cccagcttct tgatgtaatc aaaagcagtt
     3421 gactggccgt attggttaac tgttccagcc tgagcagcac ccaagaaagt tcctcgaaga
     3481 tgttcatcta cacccgatgt aggtgattta gtcaaatcac gaatgtgcat ttcacagata
     3541 actgccttac atggattttc caagcgccaa gtagcctccg aaccgtgctt aacctcgaag
     3601 ttttcaactt gcttttctac atggctcaga atagctgaac gtttgccatc agggctggtc
     3661 gcgattgtat aaggatcacg tgtcagtgtt tggtgatgag ggaattggac ttgatactga
     3721 taagtcttac ctaccaaatc ttcttcaaca tccaaactcc agacaccgat tgtattgtcc
     3781 ttatgattat aagagtagct attgcctctt ttcatctcaa aagtcttcca aacgggtgca
     3841 tcattagcag ctgattcata aacgacaact tgcacttctg tcgctgtagg tgaccagaga
     3901 gaaaaatgag cctgattgtc ctctacacgg caacccaatt ctccttggta accccaatga
     3961 tgatcaaaac tagcactgtt aatggcctta tcaaaggcaa aaggattttg atttttatag
     4021 aaaggactgg caatagcagg attttcagag taataaatcc tatcatcgcc ttccaaaatc
     4081 cagacctctg ttaatagggg atagtgatta aaacggatag aatattcttt actagtttga
     4141 cctgtatgaa ccacaaaatt caagctttct ataacatgtg aacttgggtg ttcaaagcta
     4201 aataaagctc caaaataatc ttctttgtag gttagcaaat caattcgttg atcctgactt
     4261 tttacaaagg agcaagtgtc atattctcca ttcttacgat ggtaatgaat gcgcataggg
     4321 tagttataca ttttttattt ttccttttta ctttgtttct atttcactaa taaatttttg
     4381 tcaatctcgt ctcaattaac agacatagtc atattctcta aactctgttt ttaaacgatc
     4441 cattacaaac tttctagcca tgcctcatct ctgacctgga taccaagttc ttgtgctttt
     4501 tgcagtttac ttccagcgtc tgcacctacc acgacgaggt cggtcttttt agaaatacta
     4561 cctgtcactt tggcacccag actttcgagt ttacttttag cttctgagcg cttgagtcgt
     4621 tccaattttc ctgtcaatac cacggtcaaa cctgacaagg ccgcatccgc tactaccgtc
     4681 tgtcctttat agtccagatt gaccccagtt tctttcaatt ctctgagcag aatttcagag
     4741 ccttctgtcg caaaataagt ctgaagactt ttggcaatca cgccacctag actttcaata
     4801 ctagccactt cctctgaatc tgcctgagac agattttcaa ttgaatggaa atattgaagt
     4861 aaaagctgac taaccttgct tccgacatga cgaattccca aaccaaataa gagcttctcg
     4921 gcagagtttt cctttgatgc ttggatagcc tgatacagtt tagcagcgga cttttcctta
     4981 accccctcta aaaggaggaa atcctcttct tgcaaacgat aaatatccgc cacatccttg
     5041 actaaattag cagcaaaaag cttctcaaca atagatggac caaggcctgt aatattcata
     5101 gcatcacgag aagcaaagtg aatcaagcct tccatgattt gagcagggca acgcggattg
     5161 atacaacgta gggccacttc atcttcaaag tgcaacaagt cagagttaca acttggacag
     5221 tttgtaggga tatctagttt ttcttcagaa acccgtttgg actctaccac acgtaaaacg
     5281 gcagggatga tgtcaccagc cttatataca atgaccgtat cgtcttttcg gatatctttt
     5341 tcagcaatat aatctacatt gtgcagggtc gcacggctaa cagtcgtacc ggcaagttgt
     5401 actggtgtta gattagcagt tggagttaca acaccggtac ggccaactgt ccagtcaact
     5461 gataagagtt gagcttcttt ttcttcggca gggaacttgt aggctactgc ccactttgga
     5521 gccttaacgg taaaaccaag ttcttcttga cttgctaggt cgttgacctt gattaccact
     5581 ccatcaatat cgtaaggcag attttcccgt tcctgtccta cttcttggat aaaattccag
     5641 atttcatcta tgttttcagc caagattcgc ttaggattga ccacaaaacc tagttgttct
     5701 aggtacttca aacccttttc ttggctatca cgagttgaag ggctggcttc ttgatagaga
     5761 aacgttgcaa gattacgctt ggcaactact gctgtatcca actgacgcag agttcctgct
     5821 gccgcattac gaggattagc aaattcaggc tctccatttt cttggcgcac ttggttaact
     5881 tggtcaaagg aagcgcgtgg catgtaacat tccccacgaa ctgtgatatc tagttcttct
     5941 ggcaaagtca aagggatgtc cttaacacgc ttgaggtttt ctgtgatatt ttcaccaatt
     6001 gaaccatctc cacgtgttac cccagcaacc aaaatcccct tttcataagt cagcgagata
     6061 gataagccat cgattttcag ctcacaaata taggtcggat gagccacttc cttacgaaca
     6121 cgcgcatcaa aagcatctag ctcctcacgt gaaaaagcat cctgcaaact ataaagagga
     6181 tactgatgac tgtatttttc aaaaccatct aaaaccttgc caccaacacg atgagtcgga
     6241 ctgtctgcta gcacttgctc tggataagca gtttctaact cgaccaactc acggtaaagg
     6301 cggtcatact cactgtctga aaccgaggga ttatcgctgg tatagtactc agtcgcatag
     6361 cgattgagca aagcgactaa ctcattcatt cttttattca taagaccatt ttaccataaa
     6421 acaagccctc ctcacaaacg agaagggcgg aaaaaacact tagtttgaaa ttatttttga
     6481 aactcaagca accttatatc aatttttcaa aatgagttcg aacataaata aacgatatac
     6541 aagacaagat gataacacca cttccaatta tcaggaaaga agagagatgt acacttggca
     6601 agactgtcat aaatcctttt gcaataggca taaatagaat agctaaggta aaaattgtac
     6661 tcagtactct tccaagaaat tcgctctcaa ccttggtttg tacttgagta aaaaagtgaa
     6721 tattaaaaat cgtcataaac aattcacaaa ctaaatttcc agaaaaggaa agaaaagttg
     6781 gaagtggtaa tcccatcata aaaactccga cacctgtcaa agccagtaaa atcaaaagat
     6841 tataaatatt agctttaatt ttactagcta gaagagcccc aatgatggaa ccaatagccc
     6901 ccatagttaa aatacttgca taggctcctt ctgacccgta aagctgattc gaaaagggaa
     6961 gtagaaattc aaaagctgca aaaaagaaat taacgctgga agctaccagc aaaaggaaga
     7021 aaatttcttg ctgatgccag atatagtgta acccatcctt gatatctaca aaaatatctc
     7081 tcccagtaaa agcctttttc tcttgaactt ttgcttcctc ttttggaagg aaagccacta
     7141 gaacaaaagc aatgaaaaaa gtcagcgagt ctagcagtag cgtcatatgg agacttgcaa
     7201 actgtaaaac aaggaaggaa agaacaggag agctaacacc tacaacctgc aaaaccagct
     7261 ctaggcgaga attatagatc acaatctcat ctttctccac cacttcagtt atgatagctt
     7321 tattggctgt gcgagaaaag gcaaaagcaa tagcctgcac aatgttagca acaatcaaag
     7381 cgccaatcat ccagctatca ttccttatga aagaaatagc cagacaaaga atcccacaaa
     7441 caagatctgc cgtcattaaa atcttacgac gagaaaaacg gtctgaaata actccgccaa
     7501 agggattgac gagaatagat gtgacgagct cagaaatctg atacattcct aaaactgtct
     7561 gtcctatagt ccccatagaa gccaaccaga cactatttcc ataatcatag agcatatttc
     7621 ccattttatt gatagcccca cggctaatca actgcactgc atagcgattc atattaaagc
     7681 tcctctcaaa ttttgaaact attgtatcaa aaccgaaagg agctttttat tttttccctt
     7741 atttgggaaa attaactttt gacaaatttt tcgtagtgtt cctgataata ggctacttgc
     7801 tctggaagac ctaacacatc aaaaatatgt atggcctctt gcatctgctt acagccttct
     7861 ttacactgtc ctttttgata taaggcaaaa ccttttaaat aatggaaaac attacgctca
     7921 taaagcttaa tacctttgtc aataatcttc tctgtataag cctcaaaata gttggcatta
     7981 taaaaagaag aatgctctaa acaatgctgg taacaattga gggccaaaat caacactaat
     8041 ctcttatggc tactaatctc ttggtaaaat tcctccctct ccataacttc tctaccaatc
     8101 cgagtgacat aatccacatc gtagaaacta tagaggttac cgaaaagaat caactcatac
     8161 atggtccatt cttctgtttt gaagagataa tctgctacct tatccaaatc atcctgcttc
     8221 atatcataac tcgcatctct ttgacaaatc agaccctgta gcaaaatcca gttcagctca
     8281 aaataaaggg gagtcgtcga actcttagac ttttcaagtt gttctctttg aagcttttga
     8341 aaacctgcaa tatcgtttga atagtaaagt gggataatct gtgccatcat agacacatgt
     8401 tcatgattat gaaaattcct tgccttatcc atgaaatttt cgattgttac atgaatgtta
     8461 tccaaaatct caaagaaacg ggagactgcc aggtcagact ccccaagctc aaagcgagat
     8521 aactgagagg tagagcagga ttcgcctgct gcttccttca aagaataatt tccacttgtt
     8581 cgaaattcac gaaatacttt tccaagatgt tccatcttta cacctgctct gataattctt
     8641 cccactcaag catagcttct tcctgacgat ggctgatttt gtccagctca gcctgtaatt
     8701 ccatgagttt gtcggcatcg tttgtttcca acatttgttc agaaatggct tggctttgac
     8761 tttctagctc ttcaatttca gcttctagac tttcgatttg tcgcatgagt ttgcgaactt
     8821 ctttttgact ttctttctgg gcctgatagt cattgactgg acttgcttcc tttgcttgat
     8881 tgctagttga agcttcctca gtctgactca tttctgctgt tgctttcttc tcaacatagt
     8941 agtcgtaatc tccaaggtag agagttgaac cattctcaga caattccaaa acatgagttg
     9001 ccacacgatt gataaagtaa cgatcatgac tgacaaaaag caaggtacca tcaaagtcaa
     9061 tcaaggcatt ttctagcact tccttactat caatatccaa gtgattggtc ggctcatcca
     9121 gaatcaaaaa gttattgttt tccatagaca atttagctaa aagcaaacga gctttttcgc
     9181 caccagatag catgccgact gattttttaa catcatctcc tgagaaaagg aaggctccaa
     9241 gacggttgcg gatttcaact tctggtgtca atttgaaatc attccagagt tcatccagca
     9301 ccgtattact tggtgtcagc ttgctttggg tttggtcata gtaaccaacc tcaacattag
     9361 cgccaaagcg cttttctccc ttgataaacg gaatttgatc cacgattgat ttgataaagg
     9421 ttgattttcc gataccattg ggaccaacga tagcaacagc attcatctta cgaaggtcta
     9481 ggttaatcgg ttgtgacagg acttccccat catagccaac agccgcattt tcaacagtca
     9541 aaacaacatt gcccgacgtt ttttcagact ggaaggtcat gttggctgct ttcttgccag
     9601 cttccggctt gtccaaacgc tccatttttt ctagttgttt acggcgagat tgagcacgtt
     9661 tagtcgttga agcacgaact agattgcgat tgacaaagtc ttccagagca gcgatttcct
     9721 tctgttgctt ttcatagttt tttgcctcag tagctagctt ttgctccttc aactcgacaa
     9781 aacgagagta attccccaca tagcgatcca aggaatgctt ggtcaaatct agcgtaattg
     9841 tcgcaacctt gtccaagaaa taacggtcgt ggctgacgat aatgagggca ccgctatagt
     9901 ttaccaagta attctctagc caggagatgg tttcaatatc caagtggtta gttggctcgt
     9961 ccaagaccaa gagattgggc ttttcaagga gcattttagc tagagccaaa cgagtatttt
    10021 gaccacctga aagttcagct attttcatct gccacataga ctcgtcaaac ttgaatccat
    10081 tcaaaatcgc tcgaatatca gcttcatagg taaagccacc tgcttggcga aaattctcag
    10141 ataagcggtc ataatctgac atcagtttat ccaaatcctc accagacttt tcacccatct
    10201 ccagctccat ctgacgcagt tgtctctccg tccgacgcaa atcattaaag acatgaagca
    10261 tttcatcgta gatggtattt tcagactcaa aacggctatc ttgggctagg taagacagag
    10321 aaatatcttt tttcttattg atttctccgc tagttggctc ctcttctcca actaaaatct
    10381 tcaaaagagt agacttacct gcaccatttt tcccaacaag agcaatccga tctcgttcat
    10441 caacctgcag gttgatatta tcgaaaagaa cctctcctgc aaaagaacgt tcaattttat
    10501 tagcttgtaa aataatcata agagtagtat agcatgtttc cctaaggcat tcaagataat
    10561 cgtaagtctt ttagtacaac ttttataaca taaaataaac taaattatgt atattttata
    10621 ttagattcct tcactatctt gttggatttt ctaaccagct aatcttgttt caaatagtta
    10681 tcgcacaagc ctattattta attcttttca ttatttacgt acgc
//
LOCUS       DAVLRT010000064        10705 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLRT010000064 DAVLRT010000000
VERSION     DAVLRT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.064"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            101..598
                     /locus_tag="VXM85_001614"
     CDS             101..598
                     /locus_tag="VXM85_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1346163.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            598..2253
                     /gene="dnaX"
                     /locus_tag="VXM85_001615"
     CDS             598..2253
                     /gene="dnaX"
                     /locus_tag="VXM85_001615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1346164.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLAGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2281..2475
                     /locus_tag="VXM85_001616"
     CDS             2281..2475
                     /locus_tag="VXM85_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1346165.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2625..3395
                     /gene="sufC"
                     /locus_tag="VXM85_001617"
     CDS             2625..3395
                     /gene="sufC"
                     /locus_tag="VXM85_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1346166.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3423..4685
                     /gene="sufD"
                     /locus_tag="VXM85_001618"
     CDS             3423..4685
                     /gene="sufD"
                     /locus_tag="VXM85_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1346167.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4696..5922
                     /locus_tag="VXM85_001619"
     CDS             4696..5922
                     /locus_tag="VXM85_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1346168.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPAIARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5909..6349
                     /locus_tag="VXM85_001620"
     CDS             5909..6349
                     /locus_tag="VXM85_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1346169.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6403..7815
                     /gene="sufB"
                     /locus_tag="VXM85_001621"
     CDS             6403..7815
                     /gene="sufB"
                     /locus_tag="VXM85_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1346170.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVICELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8046..9287)
                     /locus_tag="VXM85_001622"
     CDS             complement(8046..9287)
                     /locus_tag="VXM85_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1346171.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9390..10499
                     /locus_tag="VXM85_001623"
     CDS             9390..10499
                     /locus_tag="VXM85_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1346172.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3177 a         1960 c         2325 g         3243 t
ORIGIN      
        1 tggatttacc cactacaaat attatagagt ccaaaatcct ttgtttacta aacaagggat
       61 ttttcttttg tctctgctcc ttttttgata taatagttct atgttaaaat cagaaaaaca
      121 atcacgttat caaatgttaa atgaagaatt gtccttccta ttggaaggcg aaaccaatgt
      181 tttggctaat ctttccaacg ccagtgctct cataaaatca cgttttccta ataccgtatt
      241 tgcaggcttt tatttgttcg atggaaagga attggtttta ggccccttcc aaggaggtgt
      301 ttcctgcatc cgtattgcac taggcaaggg ggtttgtggt gaggcagctc actttcagga
      361 aactgttatt gttggagatg tgacgaccta tctcaactat atttcttgtg atagtctagc
      421 taaaagtgaa attgtggtgc cgatgatgaa gaatggtcag ttacttggag ttctggatct
      481 ggattcttca gagattgagg attacgatgc tatggatcga gattatttgg aacaatttgt
      541 cgctattttg cttgaaaaga caacatggga ctttacgatg tttgaggaaa aatcttaatg
      601 tatcaagcac tttatcgaaa atatagaagt caaaacttct cccagttagt tggtcaagaa
      661 gttgtggcta agactcttaa acaagcggtg gagcaagaga aaataagtca cgcttatctt
      721 ttttctggtc ctcgtggaac gggaaaaacc agtgttgcta aaatctttgc caaggctatg
      781 aactgtccca atcaagtggg tggcgaacct tgcaataact gctatatttg tcaagcagtg
      841 acggacggta gtttagaaga tgtcattgaa atggatgcag cttctaataa tggggtagat
      901 gaaattcgcg aaattcgtga taaatctacc tatgcgccta gccttgctcg ttataaggtt
      961 tatatcatag atgaggttca catgctgtct acaggggctt ttaatgccct cctaaagacg
     1021 ttggaagaac caacacagaa tgtagtcttt attttggcca ctactgaatt gcacaagatt
     1081 cctgctacta ttctatcccg tgtgcaacgt tttgagttta aatcaattaa gacacaggat
     1141 attaaggaac atattcacta tatcttagaa aaagaaaata tcagttctga accagaggct
     1201 gtggaaatca ttgccagacg ggcggaaggt ggaatgcggg acgccttgtc tattttggat
     1261 caagccctga gtttgacaca gggaaatgag ctgacgactg ctatctctga agaaattact
     1321 ggcaccatta gcctatcagc cttggatgat tatgtggcgg ccttgtctca acaggatgtt
     1381 cccaaagctt tgtcttgctt gaatcttctt tttgacaatg gtaagagcat gactcgtttt
     1441 gtgaccgatc ttttgcacta tttaagagac ttgttaattg ttcaaacagg gggagaaaat
     1501 actcatcata gttcagtctt tgtagaaaat ttggcacttc ctcaaaaaaa tctgtttgaa
     1561 atgattcgct tagcaacagt gaatttagca gatattaagt ctagtttgca gcccaagatt
     1621 tatgctgaaa tgatgaccgt ccgtttggcg gaaatcaagc ccgaaccagc tctatcagga
     1681 gcggttgaaa atgaaattgc tacgctgaga caggaagttg cccgtctcaa acaagagctt
     1741 tctaatgcag gtgcggttcc taaacaagtt gcaccagctc ctagtcgacc agctacgggc
     1801 aaaacagtct atcgtgtcga tcgcaataaa gtgcaatcta tcttacaaga ggccgtcgaa
     1861 aatcctgatt tagcacgtca aaatttaatt cgtttgcaga atgcctgggg agaggtaatt
     1921 gaaagtctag gtgggccgga caaggctctg ctagctggtt ctcaaccggt tgctgccaat
     1981 gaacaccatg ctattcttgc ttttgagtct aacttcaatg ctggtcaaac tatgaaacga
     2041 gacaatctca ataccatgtt tggtaatatc ctcagtcagg cggcaggttt ttcacctgag
     2101 attttagcta tttccatgga ggaatggaaa gaagttcgcg cagccttttc agccaaagcc
     2161 aaatcttctc aaactgaaaa agaagtagaa gaaagcctga ttccagaagg atttgaattt
     2221 ttggctgata aagtgaaggt agaggaagac taaagaaaga tttcatgata caataagttt
     2281 atgaataaac aacaatttat tattatggcg ctatttacag ctgctgagac ctattttttc
     2341 aatgaagcct ggatgactgg ccgctatatt atggcagcct tttgggcaat tttactcttt
     2401 agaaatttcc gagtcagtta tgtgatgggt aaaatcgttg atgtcatcga tcagcatttt
     2461 aataggaaag actagccctc agcttccaga caaaatcaaa gccttttagg cttttttttg
     2521 ttatactaga aaagtatatt tatagaattt ttgctctatt tctggggaaa tcagacgttt
     2581 ttctagtaag tactgtaaaa gttttgaaaa agaaaggaac tatcatgtca gtaatagaga
     2641 tcaaagatct tcacgttgag attgaaggaa aagaaatttt aaaaggggtt aacctgaccc
     2701 tgaaaacagg agaaattgcc gctatcatgg gaccaaatgg tacaggtaaa tcgactcttt
     2761 ctgccgctat catgggaaat ccaaactatg aagtaactaa aggtgaagtt ttgtttgatg
     2821 gcgtaaacat ccttgagttg gaagtggatg agcgtgcgcg tatgggactt ttccttgcta
     2881 tgcaataccc atcagaaatc cctggaatta ccaatgctga atttcttcgt gccgctatga
     2941 atgcgggtaa agaagatgat gagaagattt cagttcgtga gtttattact aagctagatg
     3001 aaaaaatgga attgcttaac atgaaagaag aaatggcaga gcgttacctc aacgaaggct
     3061 tctctggtgg tgagaaaaaa cgcaatgaaa ttcttcaact tttgatgttg gagccaacat
     3121 ttgctctttt ggacgagatt gactcaggtc ttgatattga cgctcttaaa gttgtgtcta
     3181 aaggtgtcaa tgccatgcgt ggtgaaggtt ttggtgctat gatcatcact cactaccaac
     3241 gtcttttgaa ctatatcaca cctgatgtgg tacacgtgat gatggaaggt cgtgttgtcc
     3301 tttctggtgg tccagaattg gctgcgcgtt tggaacgtga aggatacgca aaattagctg
     3361 aagaacttgg ctacgactac aaggaagaat tgtaattccc tcgtatcttt taggagaagt
     3421 aaatgactag agaaaatatt aaactttttt cagaaatgca cgctgaacca agctggttgg
     3481 ctgatctccg tcaaaaagct tttgacaaga ttgagacttt ggaattacca gttattgagc
     3541 gtgtcaaatt ccaccgttgg aatctgggtg atggaacgat tacagaaaat gagccatcag
     3601 caaatgttcc agatttcaca gctttagatc atcacttgaa gttggtgcaa gtaggaactc
     3661 aaactgtttt cgaacaaact ccagttgagt tagctgaaca gggtgttgtc ttcacagact
     3721 ttcactcagc tttagaagaa attccagagc tgatcgaaga attcttcatg tcatctgtta
     3781 agtatgatga tgacaagttg gcggcttacc acacagctta ctttaacagt ggtgctgtac
     3841 tctatattcc agataacgta gaaatcacag agccaattga aggaattttc taccaagata
     3901 gcgatagcaa tgtgccgttt aacaagcata ttatgattat cgctggtaaa aattctaaga
     3961 ttaattatct ggagcgttta gagtcacgcg gtgaaggaag tgccaaagca actgctaata
     4021 tcacagtaga agtgattgca cgttcaggtg cgcaagtcaa gtttgctgct atcgaccgac
     4081 taggtgaaaa cgtcactgcc tacattagcc gtcgtggtaa attaggcaac gatgcaagta
     4141 ttgactgggc tatcggtgtc atgaacgaag gaaatgtcgt tgctgatttt gatagtgact
     4201 tgattggtaa tggtagccat gctgacctca aggttgtagc tctttcaagt ggtcgtcagg
     4261 tacaagggat tgatactcgt gtaactaact atggctgcaa ctctatcgga aatatcctac
     4321 aacatggggt tatccttgaa aaagcaactt tgactttcaa tggtatcggc cacatcatca
     4381 agggtgctaa gggagcagat gcccaacaag agagccgtgt tctcatgctt tcagaccaag
     4441 cgcgttcaga tgctaaccca attcttttga ttgatgaaaa tgatgtcact gcaggacacg
     4501 cagcgtctat cggacaagtt gatccagaag acatgtatta cctcatgagt cgtggattgg
     4561 ataaggcaac tgcagagcgt ttggttgttc gtggtttcct tggatctgtt atcgtggaga
     4621 ttccagtcaa ggaagttcgt gatgaaatga ttgcaactat cgaagagaaa ttgtcaaaac
     4681 gctaaggggc agcctatgtt agatgtagaa gcgattcgca aggattttcc aattttagat
     4741 cagattgtca atgatgaacc tctggtctat ctggacaatg ctgcgacgac acaaaaacca
     4801 ctagtagttc tgaaagctat taacagctac tatgagcagg acaatgccaa tgttcaccgt
     4861 ggtgtccata ccttagcgga acgagcgaca gcttcttatg aagctgctcg tgaaaccatt
     4921 cgtaagttta ttaatgcagg ctctacaaag gaagttctct ttaccagagg aacgacaacc
     4981 agccttaact gggtggcacg ctttgctgag gaaattctca ctgagggaga ccaggtcttg
     5041 atttcagtaa tggaacacca ttctaatatc attccatggc aggaagcttg tcgaaagact
     5101 ggagcagagc ttgtctatgt ctatcttaaa gacggtgcct tggatatgga ggatttgcga
     5161 gctaaattga ctgataaggt taaatttgtt tccctagctc atgcctccaa tgttcttggt
     5221 gtggtcaatc cgatcaagga aatcactcaa ttagcccacc aagttggggc aatcatggta
     5281 gtggatggtg ctcaatctac acctcatatg aagattgatg tccaggactt ggatctggac
     5341 tttttcgcct tttcgggtca caagatggct ggtccgactg gtatcggtgt cctttacggc
     5401 aaagaaaagt atcttgagca aatgtctcca gtagaatttg gcggcgagat gattgatttt
     5461 gtctacgagc aatctgctag ttggaaggaa ttgccttgga aatttgaggc tggaacgcca
     5521 aatatggcag gagctattgg acttgcgact gcagttgatt atctggaaaa gattggtatg
     5581 gatgccattg aagctcatga acaggaattg attgcgtacg tctatccaaa actgcaggca
     5641 attgagggat tgaccattta cggttctcag gatttggctc aacgttcggg tgttattgcc
     5701 tttaacctag gtgatctcca tcctcacgat cttgcgacgg ctctggatta tgaaggagtg
     5761 gctgttcgtg ctggtcacca ttgtgcgcaa cccttgcttc agtatttgga agtcccagca
     5821 atagctcgtg caagttttta tatctacaat accaaggcag attgcgacaa actagtcgat
     5881 gccctacaaa agacaaagga gtttttcaat ggcactttct aaactagata gcctttatat
     5941 ggcagtggta gcagaccatt cgaaaaatcc acatcaccaa gggaagttag aagacgctga
     6001 gcaaatcagt ctcaacaatc cgacttgtgg ggatgtcatc aacctctctg tcaagtttga
     6061 tgcagaggac cgtttggaag atattgcttt tctaaattca ggatgcacga tttcaactgc
     6121 ttctgctagt atgatgacag atgccgtttt aggaaaaacc aaacaagaaa ttttagaact
     6181 ggcgactatt ttttctgaaa tggttcaagg gcaaaaagat gagcgtcaag accaacttgg
     6241 agacgcggca ttcttgtcag gtgttgccaa attccctcaa agaatcaagt gtgcaaccct
     6301 agcttggaat gcccttaaga aaacaattga aaatcaagaa aaacagtaag acaagtttct
     6361 tttgtcttat gaattattag aaatgaagaa agaaaggata ttatggctga agaaagagta
     6421 gaaccaaaac caattgacct tggtgaatat aaatttggtt tccatgacga tgtagagcct
     6481 gtcttatcga caggaaaagg actcaacgaa ggtgttattt gtgaattatc tgctgctaag
     6541 ggtgagcctg agtggatgtt ggagttccgt ttgaagtctt atgaaacctt caaaaaaatg
     6601 cccatgcaaa cttggggagc agacttgtca gagattgact ttgatgactt aatctactac
     6661 caaaaaccat ctgacaaacc agcccgttct tgggatgatg tacctgaaaa gattaaagaa
     6721 acctttgaac gtatcgggat tccagaagct gaacgtgctt atttagcagg ggcttctgcc
     6781 cagtacgagt cagaagtggt ttaccacaac atgaaggaag agttccaaaa attaggtatt
     6841 atctttacag atacagattc cgcactcaag gaatacccag acttatttaa acaatacttt
     6901 gcgaagttgg taccgccgac agataacaag ttggcagccc tcaactcagc agtatggtcg
     6961 ggtggaactt ttatctacgt gccaaaaggt gtcaaggtag atattccact tcaaacttat
     7021 ttccgtatca ataacgaaaa tataggtcag ttcgaacgta ccttgattat cgttgatgag
     7081 ggagcaagcg tccactacgt agaaggatgt acagcaccaa catattcaag caatagctta
     7141 cacgctgcca ttgtagaaat ttttgctttg gacggagctt atatgcgtta tacaactatc
     7201 caaaactggt ctgataacgt ctataacttg gtaacaaagc gtgctaaggc tcaaaaggat
     7261 gccactgttg agtggattga tggaaacttg ggtgccaaaa cgactatgaa atatccatct
     7321 gtttaccttg atggagaagg agcgcgtggt accatgctct ctatcgcctt tgctaatgca
     7381 gggcaacacc aagacacggg tgctaagatg attcacaatg ctccacatac cagctcgtct
     7441 attgtgtcta aatccatcgc taaaggtgga ggaaaggttg actaccgtgg acaagtcacc
     7501 tttaacaaga actctaagaa atctgtttcc cacattgaat gtgataccat tatcatggat
     7561 gacttgtcag catcagatac tattccattt aatgaaattc acaactcgca agtggctttg
     7621 gaacacgaag ccaaagtatc taagatttca gaagagcaat tgtattatct catgagccgt
     7681 ggattgtcag aatctgaggc aactgaaatg attgtcatgg gatttgtaga accctttaca
     7741 aaagaacttc caatggaata cgcagttgag ctgaaccgct tgattagcta tgaaatggag
     7801 ggatcagttg gataaaattt gattttatgc tcttcgaaaa tctcttcaaa ccacgtcagc
     7861 atcgccttac cgtatgtatg gttactgact tcgtcagttt catctacaac ctcaaaacag
     7921 tgttttgagc aacctgcggc tagcttccta gtttgttctt tgattttgag tattagattt
     7981 actcaaaatc aaggattttg aagatgaact tgtatcaaaa aatcgcggtt taaaatcgcg
     8041 attttttata atttctcgtt aacaaagcgg acaaactgat tccaccaaac ttttaagaag
     8101 aaggcttttt caattttctt gtctgctacc atttcgaaac tagggcgctc tgtggtgatg
     8161 taaccttgac caatcaagtc cttgtcttca taagtcaaat ggccaaccac tgttccagct
     8221 tcaagtggtg ctgggattgc tttggaatca ggtgtgaatt gaacagattg ggaagattga
     8281 ttcccaacac gttcgattag atagatatcc tctggagcca ctgcagttac tgtatcttct
     8341 tttccatctt gtacaggggc tttgctatct tgataggcat cgccttgttg aacgattttg
     8401 cgaagtgtaa atgtagaaga aatataatcc attagggaag atgtagctgt aaatcgagcg
     8461 taaggattat tgtcttgatg atctgcattt aaaacaactg tgatgactct catgcctttt
     8521 tcgacagtag taccaacaaa agactctcca gccttatctg ttgttcctgt ttttagccca
     8581 tcaaaaccac cacggtaagc aggcatacct tctaacatgt agttggttga agtgattgtc
     8641 atcccagcaa aagtagaaga tggttttttg gtgatttcta agacttgtgg gtattttttg
     8701 atgaggttgc gagcaacgat agcgacatca taagcactaa gcttattttc ctcatctttt
     8761 ttagaacctg ggtaaatgtt atcccctaga gtttcattgt taagacctgt cgtattgaca
     8821 acagtggcat cctgaattcc ccattccaag agttttgccc gcatcatatc gacgaaatct
     8881 ttttctgagc cagcaatttt ctcagctagg gcaatagcgg cgctgttggc actagatacc
     8941 agagttgctt caagcaactc ttcgacagta taattacggg cctccatagg aatattactg
     9001 gcttcagaat ttgtcgtcaa ttgataagga taatcagaaa tatctacagg agtggagagg
     9061 gtaatacttc cgttttccaa agcttcatag accagataaa cagtaatcaa ttttgttatg
     9121 gaagcaattt cgacaggttg cgttgcatcc ttctcataga gaattttacc agtatttgcc
     9181 tcaacagcaa tcgcatgttt agcggcaatg gtaaaatctt gagcaacagc agtagaagca
     9241 ccccctaaaa gagagacagt taacaaagtt aaaaatattt ttttcatagt agtcttattc
     9301 tatcataaag aaaaaaaata ttcttgcttt aataattcat ctgttaagct ttttgaaaat
     9361 atggtaaaat aaagtgaggg aggtaactca tgtttcgtag aaataaatta tttttttgga
     9421 ccacagaaat tttactctta accatcatct tttacctatg gagacagatg gggtctttga
     9481 ttaacccttt tgttagcgtg cttaatacaa ttatgattcc atttttatta gggggctttc
     9541 tttattattt gacaaaccct attgttactt tcttaaataa agtctgtaaa ctcaatcgtt
     9601 tgcttggtat tttaattacc ttgtgtactt tggtctgggg aatggtcata ggtgttgtct
     9661 atctcttacc tattttgatt aatcagttat ctagtttgat tatatctagt caaactattt
     9721 atagtcgagt acaagactta atcatagact tatctaatta tcctgcgctc cagaatttgg
     9781 atgtagaagc tacaattcag cagttaaact tatcctatgt tgatattctt caaaatatcc
     9841 taaatagcgt atcaaatagt gtggggagcg tcttgtcagc tcttatcagt actgttttga
     9901 ttttgattat gactccagtt tttttggttt atttcttatt agatggacat aaattcttgc
     9961 ccatgcttga aagaacgatt ctaaagaggg atcgcttgca tattgcaggc ttattaaaga
    10021 atttaaatgc gacgattgct cgctatatta gtggagtttc gattgacgca atcattatag
    10081 gttgtttggc ttatattggc tatagtatta ttggtttaaa atatgcttta gtttttgcca
    10141 ttttttctgg tgtagccaat ttaattcctt atgtggggcc aagtattggt ttgattccta
    10201 tgatcatcgc aaatatattc actgatcccc atagactgct gattgcagtg atttatatgc
    10261 ttgttgttca gcaggtagat ggcaatatct tatatcctcg aattgtagga agtgttatga
    10321 aggttcatcc aatcacgatt ttagttttac ttttgttgtc aagcaatatc tatggtgtag
    10381 ttggaatgat tgtcgcagtg ccaacctatt ctatcttgaa agaaatttct aagttcttat
    10441 cccgtttgta tgaaaatcat aaaataatga aagaacgaga aagagaatta gctaagtaaa
    10501 agtcaggaga accctgattt ttctttactg gaagtggcct ttagattaga agactgaaaa
    10561 taagttaaag tcttaaacta attttcacag ctaagaatag tagaagttaa tctgataaaa
    10621 atcgaaaaaa ccagtggaat tccgtgtcag ggtaagttcc actggttttc atagtctatt
    10681 aaagttcgaa tgaaacctat ttata
//
LOCUS       DAVLRT010000065        10467 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLRT010000065 DAVLRT010000000
VERSION     DAVLRT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.065"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            1..581
                     /locus_tag="VXM85_001624"
                     /pseudo
     CDS             1..581
                     /locus_tag="VXM85_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            547..927
                     /locus_tag="VXM85_001625"
     CDS             547..927
                     /locus_tag="VXM85_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1346173.1"
                     /translation="MRLFCLVLVSIDYINCLSETTDKNWHKSDRVFVTNTGKTVHSSI
                     LSKSLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVGHSDSEVTTSI
                     YTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1065..2369)
                     /gene="eno"
                     /locus_tag="VXM85_001626"
     CDS             complement(1065..2369)
                     /gene="eno"
                     /locus_tag="VXM85_001626"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1346174.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2533..2979
                     /locus_tag="VXM85_001627"
     CDS             2533..2979
                     /locus_tag="VXM85_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1346175.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2976..4091)
                     /locus_tag="VXM85_001628"
     CDS             complement(2976..4091)
                     /locus_tag="VXM85_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1346176.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASDQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4100..4429)
                     /locus_tag="VXM85_001629"
     CDS             complement(4100..4429)
                     /locus_tag="VXM85_001629"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1346177.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4374..4745)
                     /locus_tag="VXM85_001630"
     CDS             complement(4374..4745)
                     /locus_tag="VXM85_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1346178.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4804..6237)
                     /gene="glgA"
                     /locus_tag="VXM85_001631"
     CDS             complement(4804..6237)
                     /gene="glgA"
                     /locus_tag="VXM85_001631"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1346179.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHIHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWLAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6234..7373)
                     /gene="glgD"
                     /locus_tag="VXM85_001632"
     CDS             complement(6234..7373)
                     /gene="glgD"
                     /locus_tag="VXM85_001632"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1346180.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7363..8505)
                     /locus_tag="VXM85_001633"
     CDS             complement(7363..8505)
                     /locus_tag="VXM85_001633"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1346181.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8495..10423)
                     /gene="glgB"
                     /locus_tag="VXM85_001634"
     CDS             complement(8495..10423)
                     /gene="glgB"
                     /locus_tag="VXM85_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1346182.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPNVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLICIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3173 a         2304 c         1867 g         3123 t
ORIGIN      
        1 taccatccac tatacgctca aagctatggg ctacactcga aaaaaagaac cacacctact
       61 atgaacaaga cccagaaaaa gtagccttat ttcttaagaa ttttaatagt ttaaagcacc
      121 tagcacctgt ttagattgac gaaacaggat tcgatactta tttttatcga gaatatggtc
      181 gctcattaaa aggtcagtta ataagaggta aagtatctgg aagaagatat cagaggattt
      241 ctttggttgc aggtctaaca aatggtgagt taatcgttcc aatgacttac gaagagacga
      301 tgacgagcga cttttttgaa gcttggtttc agaagttttt cttaccaaca ttaaccacac
      361 catcggttat tattatggat aatgcaagat tccatagaat gggtaagtta gaactcttgt
      421 gtgaagagtt tgggtataaa cttttacctc ttcctcccta ctcacctgag tacaatccta
      481 ttgagaaaac atgggctcat atcaaaaagc acctcaaaaa ggtattacca agttgcaata
      541 ccttttatga ggctcttttg tcttgttctt gtttcaattg actatataaa ttgtctaagc
      601 gaaacaaccg ataagaattg gcacaaaagc gaccgtgttt ttgttaccaa tacaggaaaa
      661 acagttcata gttctatctt gagcaagtct ctccagcgag caaacgaacg ccttaaaaaa
      721 ccaattccca aacatctgtc ccctcacatc ttcagacaca ccactattag catcttatca
      781 gaaaataaaa ttcctttaaa aacaatcacg gacagggttg gtcattccga ctctgaagtc
      841 actacttcca tctacaccca cgtcacaaag aacatgaaag atgaagcaat caatgtactg
      901 gataaagtta tgaaaaagat tttttaaaaa gttttgcccc ttttttgtcc tctaaataca
      961 aaaatagccc ttcggataaa atccgagggg ctagaaacgt tgttaaatca acggccgaac
     1021 ttttgaattt catggttcgg gataaaatag ttcactgaac tattttattt tttaaggttg
     1081 tagaatgatt tcaatccacg atattcagct acttcaccaa gttggtcttc gatacgaagc
     1141 aattggttgt atttagcgat acggtctgta cgtgaaagtg aaccagtctt gatttgtcct
     1201 gcgttagttg caactgcaat atcagcgatt gttgaatctt cagtttcacc tgaacggtgt
     1261 gatacaacag cagtgtaacc agcttcttta gccatttcga tagcttcaaa agtttcagta
     1321 agagtaccga tttggttaac tttgataagg attgagttag cagcaccttc ttggatacca
     1381 cgtgcaaggt agtcagtgtt tgttacgaag aagtcgtcac caacaagttg tactttctta
     1441 ccaagacgtt cagtaagagc tttccaacca tcccagtcgt tttcatccat accatcttca
     1501 atagtgatga ttgggtattt gttaaccaat tcttcaaggt agtcgatttg ttctgcagat
     1561 gtacgaacag cagcaccttc accttcaaat ttagtgtagt cgtaaacttt acgttcttta
     1621 tcgtagaatt ctgatgaagc acagtcaaat ccgataaata cgtctttacc tggtacatat
     1681 ccagcagctt caatcgcagc aaggatagtt tcaacaccat cttcagttcc ttcgaaacga
     1741 ggagcgaatc caccttcgtc acctacggca gtttccaaac cacgtgattt aaggattttc
     1801 ttaagagcgt ggaagatttc agcaccgtaa cgaagggctt ctttaaatgt tggcgcacca
     1861 actggcaaga tcatgaactc ttggaaagcg attggagcgt cagagtgaga accaccgttg
     1921 atgatgttca tcattggagt tggaagaact ttagtgttga atccaccaag gtagctgtaa
     1981 agtgggattt caaggtagtc agcagcagca cgagctacag cgatagacac accgaggatt
     2041 gcattcgcac ccaatttacc tttgttagga gtaccgtcaa gtgcgatcat agcacggtca
     2101 atagcttgtt gatcacgtac atcgtagcca atgatagctt cagcaatgat gttgtttacg
     2161 ttgtcaacag ctttttgtgt accaagacca ccgtaacgag atttgtcacc gtcgcgaagt
     2221 tcaactgctt cgtgttcacc agtagaagct cctgatggaa ccataccacg tccgaaagca
     2281 cctgattcag tgtaaacttc tacttcaagt gttgggttac cgcgtgagtc taggacttcg
     2341 cgagcgtaaa catcagtaat aattgacatt ttttactctc cttatgagtt aaatttttta
     2401 cacctctata ataccttaaa acccctcctt tttcaagaaa aaacgttatc tttgtgcaac
     2461 ttttccttaa ctttataaag taatcgcttt cttttgtctg ttttattcta acttttatga
     2521 tatactgttt tcatgacaga tttatcaaaa caattacttg aaaaagctca tggtgggtta
     2581 aaaataaatc cggatgaaca aagacgctat cttggtactt ttgaggaaag agttcttgga
     2641 tatgtagata ttgacacagc aaatagccct cagttagaaa aaggcttttt atttatttta
     2701 gaaaaccttc aggaaaaagc agagccacta tttgtgaaga tttcaccaac tatcgaattt
     2761 gataagcaag ttttctactt aaaagaagca aaagaaactg atagtcaagc caccatagta
     2821 tctgaagagc atattacttc tccttttggc ctggttattc atagcaatgc accagttcaa
     2881 gtagaagaaa aagaccttcg acttgctttt ccaaaacttt gggaagttaa aaaggaagaa
     2941 ccagccaaaa catccttatg gaagaaatgg tttagctaaa tcttgcacat atttaataag
     3001 tgcccaatat tggcagccgt gcgctccaga tagagactgg catttttcaa actatcttct
     3061 aaaggttcac ttttctccaa aatagaaaag acagcttgga tattttcaaa tggtagggga
     3121 ggtaaatctt cagcaagact accgcaaata gcaataacag gaactccaac aggggttctt
     3181 tttgcaacac ctataggcgc tttcccagca aagctttgac tatcaagtct tccttctcca
     3241 acaacaacca agtcagcatc tgaaactttc ttatcaaagt tgattaagtc caagcaggta
     3301 tcaattccag acacgatact tgcctgagca aaggcacaca aaccaccagc aaggcctcca
     3361 cctgctcctg ctcctttaat ttctaatgtt gcaggtgaga atttttcata aaaatcttgg
     3421 atcgcctgat ctacgactgc aaacatagtc ggatgtagac ctttttgatt gccaaaagtg
     3481 taagtcgcac cttgatgacc acataaggga ctcacgacat ctgctagaat atgaatttga
     3541 acaccttcag gaattttata gcaattttct gttgaaacag aagctaagtt taataaggat
     3601 tgatcggaag caggcaagac atttccatcc ctatcataaa attgataacc taaaccagca
     3661 gcaatcccca gtcctccatc attactggcc gtgccaccaa caccgatata aatatcttta
     3721 atccctttag aaatgagatg gagaatcaac tctccaatac cacaagtttg gatttgaagt
     3781 ggatttcgtt tctctagcgg aatttttcca agaccaacca agtcagctac ttcaaatagt
     3841 gccagttccc ctttttgaaa atagcgcatg gcttcttttt gtccaaaagg gtctgtcact
     3901 tggatccatt tttcttttag gtcaagagaa tgtcggatag catctacagt accttctccc
     3961 ccatcaccaa cagggcagag gagacattct acatctgcta tcgattgttg gaagcctctt
     4021 tttattgctt cagctacctg ttgagctgtc aagctttcct taaacgaatc cggtgcaatt
     4081 acaatcttca tattttccct cattctaaac agtcaatcaa aggaagaact tctaaaaaat
     4141 ccctcttgtc aacatgatgt ggtatttctt ttttgagcat ttctttggaa caaaaggcga
     4201 ttcctaactc cgccgacttc aacattaata gattattgac cccatcaccg attgccaccg
     4261 ttctttcttt agaaagtttt agtttctttc tccatttttc cagagtttct tttttgacct
     4321 ggggacttat aatttgtcca actaattttc ctgttaaaag accttctttg acttcaagct
     4381 ggttggcagt gaaataggca ataccaaggg attttgctaa tctctccaac tattggtgta
     4441 aatccaccag acaccagacc aactaggatg ccattctttt ggagaataga gatgaactct
     4501 gggacattta gcgatagatg aattgagttg aagacgttat caaagaccaa aataggaaga
     4561 ccttccaaca aggacactct ttttcttaaa ctgctttcaa agaccaactc tcctcgcatt
     4621 gctcgacttg taatctgcga aatttccgcc tcatgacctg cctctctccc taaaagatca
     4681 atcacttctt ctaggattaa ggttccatct acatccaaaa cacacaagcc ttttacttga
     4741 gacatcagtt ctcctctcta aacagcctaa aaatcgtatg aagtcatcat acgattttat
     4801 ctattaatta actaaactat ggtacaagtc aaggtatgac ttgcaggctg tatcccatga
     4861 gaagtcactc tccatagctt gtttttgtag gtttctccaa atgtctggat ggtttctata
     4921 caagtccaat gctgtttgga aagtccaatt taaccaataa ggagatagat tgtcaaagct
     4981 aaagccagta ccgcttcctt cgattggatt gaaagcgcga actgtatctc gcaagcctcc
     5041 aacttcatgg accaatggca aggttccata acgcatagcc atcatttgag acaagccaca
     5101 cggttcaaaa cgacttggca tgaggaagag gtcacaagca gcgtagattt cctgagcaag
     5161 tttgacatca aaagtgatat ttgttgatag cttgtctggg taaatctgag ccaaccatga
     5221 gaaagctcct tcaaaggctg gatcgccagt tcccaaaaga acaatctgaa catcttcttg
     5281 caagatatgg tgaagacttt cgaccaccac atcaaaacct ttttgacgtg tcaaacgaga
     5341 aacaattccc accagtggaa cgtctgctct aacaggcaag ccaactcttt cttgcaattt
     5401 tgccttattt ttggctttcc cagacaaatc ttcctgattg aaatgatagt ctaaaagagc
     5461 atccgtctga ggattataaa gatcagcatc aatcccattc acgataccag atactttacc
     5521 agactccatt ttaagaatct gatccaaatt acatccaaac tgactagtca taatttcatg
     5581 agcatagcta ggtgaaacgg ttgaaacacg gttcgcatag agaatacctg ccttcatcca
     5641 gttcagacag ttgttccatc gaagggtgcc atcagcgtaa cgttcaaggc caactccaaa
     5701 caaatcaccc aacattcctt ctgaaaattg tccttggaat tctaaattat gaatggttaa
     5761 aactgtttca atgtcctcat aggcttgaat ccaacggtat ttttccttca acaagaaagg
     5821 aatcatagct gtatggtagt catgaatatg gagaagatca ggaataaagt caatcctttc
     5881 catagcctca atggcagcca gttggaaaaa ggcaaagcgt tctccgtcat caaaatcacc
     5941 gtaaacatga ccacggaaga aataatattg attgtcaata aagtagaagg ttacaccatt
     6001 taatactgtt ttcttaattc cacaatactg tctgcgccaa ccaacgctca cctcaaaatg
     6061 aagcacatct tcaatctgat ttccaaattt agcctctacc atatcatagt agggtaaaat
     6121 cactgcaact tcgtgcccag cttttaccag tgattttgga agagcgccaa tgacgtctcc
     6181 caaaccacct gtttttgaaa agggtgcacc ctctgctgct acaaataaaa ttttcatgaa
     6241 tgaatatcct ctgttacttt agcacctttc ttaaccacaa ctggatgttc tgcagttcct
     6301 cgaatcacaa caccaggctc aacttcaacc cctttgtcca agatagcata ttcgacctga
     6361 gccccttctc caataacaac acgagggaag agcaggctat ctttaaccaa gctatcctta
     6421 tggacatgaa tattacgtga tagaacagaa ttagctactt gaccttcaat aatactacca
     6481 gaggcaaact gagaagtgct taccttagat gtattagcat agtaagttgg ctcttcgttt
     6541 ttgacctttg tataaatctt ttggtttggt gagaaaagag aatagaattt ttgtgattca
     6601 agcatatcga tattcgcttg ataataagat ttaacagagt gaatattggc tagatagccc
     6661 gtgtactcgt aggcgaaagc tccctctttt acagccaaat cccgtaaaac atagcgcaat
     6721 ttctttggat gttctttttt agcttcttct tccaagtgtt caatcaacca aggtgtatca
     6781 acgacaaaga tatctgtaga catattgaac gtttcagctg ttgacttgct atcaaagagt
     6841 ttatgagaaa gaacatggtc tgtttcatct acatccaaga ttgcatttac ttctgaaata
     6901 tctttcttag ctagtttttt ataaactaca gtgataggct cttttgttgt actatgtagg
     6961 tggaaaactt ggttcaaatc aatgttaata agaacatcgc agttgagggc aaccgtttgg
     7021 tttgagccag aacgtttcaa ataagtaaga agctgttggt agtattcttt tccaactgta
     7081 ctactttcta cacgggtatt gtaaattcct agatagtaat gactaagaag ggttgataag
     7141 ccccactcgc gtcctgaacg aatatggtca aatactgagc tgatattatc ctgctggaaa
     7201 ataccaaaga cactacgaac acctgcatta gcaaggcttg aaagtgggaa gtcaatcaaa
     7261 cgatatttcc caccaaatgg caaacttgct actggacggt ggtccgtcaa tgtcgacata
     7321 ttgtgaaaac caactgtatt tcctaaaatg gcagaatatt tatcaatctt catctgttgc
     7381 tacccccact acttcattat atcctacaac ttgtacttca tctgttccat caatttcgac
     7441 accgtcagaa ataatcgcac cttcaccaat aatggcacgt ttaatcttag ctccttgacc
     7501 aatgatagct ccactcatga taactgaatc aaggacttcc gctccttcgc gaacttgcgc
     7561 gcctgttgaa aggatagaat gtttaacagt tccatcaacg aaacatccgt ctacaactaa
     7621 tgagtcttcc acatgagcat ttgccccgag gaagtttggt ggtgaaatca agtttcttga
     7681 gtaaatcttc cattgacggt tacgactatc caaggcattt tctggagaaa tatactccat
     7741 gttcgcttcc caaagtgact caatagtacc aacatctttc caataaccac taaattcgta
     7801 agcataaaca ctttcacctg actcaaggta atttggaatg acatttttcc caaagtctga
     7861 catgccaacc ttgctctttt cagcagcgac taacatatta cgaaggcgtt gccaatcaaa
     7921 aatgtagatt cccatagaag cttttgtaga tttaggttga gctggttttt cttcaaattc
     7981 aacaatacga ttgttagcat ctgtgttcat gataccaaaa cggcttgctt ctttaagagg
     8041 gacgtctaaa actgctactg tcaagctggc attattatcc ttatgagact ggagcatatc
     8101 atcatagtcc attttgtaga tgtgatcccc agacaaaatc aagacatact caggattgac
     8161 actgtcgata tagtcgatat tttggtaaat agcgtgacta gtcccctcaa accaacgatt
     8221 tccttcactt gcagaataag gttgaagaat agagacacct gaattgatac cgtctagtcc
     8281 ccagcttgaa ccattcccaa tatggttgtt gagagcaagt ggttgatact gtgtaacgac
     8341 cccaacatta tgaatccctg agttggcaca gtttgatagg gcaaagtcaa tgatacggta
     8401 gcgcccacca aattgcacag ctggttttgc gatgctttga gtgagtttac cgagacgagt
     8461 tccttgccca ccagcaagaa tcaaagctaa catttcattt ttcattttct actccttttt
     8521 ggtttttatt tgtgacggtt ttagtagatt tcaagcgacg tttgattttc catacacttg
     8581 ctcccatagc cggtagagta aaggttaagg tctgctcata atctttccat agtccttctt
     8641 gcgtttgaac agtttgatta tgttctttcc aaacgcctcc ccactcttcc aactcagtat
     8701 tccatacttc ttcgtaaatt cctgcaacgg gtagtccgat tgtaaaatct ttccgctcaa
     8761 caggtaccat gttaaagata cagattaaca tttctccctt tttaccctta cgaataaagg
     8821 aaagaacact ctggtctcga ttatccgcat caatgatttc aataccatca tagctggtat
     8881 cgatttccca cagacagcga tgatctttgt aaaactggtt tagctgagaa gtgaaatact
     8941 tcatcttagc attcattggg tcttctaggt tagaccattc caactgttct tcagatttcc
     9001 attctaggaa ttgaccgtat tcgctaccca tgaagagcaa tttcttacca gggtgacaaa
     9061 tttggtacgt atagagattg cgcaagcctg cgaattgatt gtaacgatct ccccacatct
     9121 tatgcatcat actcttcttg ccatgaacca cttcatcgtg cgagaatggc aagagataat
     9181 tctccttgaa aacatacata aagctgaaag tcaccaggtt aaagtcatat ttacgataga
     9241 tcggatcttc ttcgtagaaa cggaggatat cattcatcca gcccatgttc catttgtagt
     9301 caaatcctag accaccaatc tctttcattc ccgtaatctt ggtcgcagac gaactttctt
     9361 ctgcaatcat catcacattt ggatattcta acttaataac ctcattcaag cgctgaagga
     9421 aataataacc ttcatagttg agatttccgc catctttatt aggtgtccat ggagcatcat
     9481 catagtccaa atagagcatg ttgctaacag catccacacg aataccatcc aaatgataga
     9541 catcaatcca atgcttaatg caagaaatta agaaggactg gacttcattt tttccaaggt
     9601 caaaattaag ggcaccccaa ccatggttat gagccttatt atggtcttgg tattcaaaag
     9661 tcggtgtccc atcataatag gctaaggcat catcgttgat ggtaaagtga cctggtaccc
     9721 agtccacaat aaccccaata ttatgggtat gacactcctc gacaaaatct tgaaactcct
     9781 ctggtcggcc ataagcatgc tctaaagcga agtaacccat aagctgatac ccccaactca
     9841 agcccaaagg atgggacatc aagggcataa actcaatatg agtatagttc atttcaacga
     9901 gataaggaat gagttcatcc ttgagctggg caaaactata aggactgcca tcagaatttc
     9961 ttttccatga tccagcgtga acttcataaa tattgacagg acgctcttca aagccccaac
    10021 gttttcttcg tgccagccaa agtccatcct tccatttctt ctcaggaagc tctgttaaga
    10081 ttgcccctgt tcctggacga gcctcatacc tgacagcaaa agggtcaatc ttcatcagtt
    10141 gatgaccatt ttgacgtgtg acatgatatt tgtaaatatg cccttcttga gccatattgg
    10201 taaagacttc ccagacccca aaatcatttc ttaccattgg aatctgattt tcaatccagt
    10261 tggtaaaatc accaaccaag tgaacagcct gagcattagg tgcccaaaca cggaaggtat
    10321 agccatgctc tccatttagt tcttccctat gtgctcctag ataatgttgg agataaaaat
    10381 tttcacccgt cataaaggtt tttaatgctt ctctattatc catatactcc ccttctcctg
    10441 taagcgtttt ctatgttttt attatac
//
LOCUS       DAVLRT010000066        10216 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLRT010000066 DAVLRT010000000
VERSION     DAVLRT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.066"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(287..679)
                     /gene="rpsI"
                     /locus_tag="VXM85_001635"
     CDS             complement(287..679)
                     /gene="rpsI"
                     /locus_tag="VXM85_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1346183.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(699..1145)
                     /gene="rplM"
                     /locus_tag="VXM85_001636"
     CDS             complement(699..1145)
                     /gene="rplM"
                     /locus_tag="VXM85_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1346184.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1501..1818
                     /locus_tag="VXM85_001637"
     CDS             1501..1818
                     /locus_tag="VXM85_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1346185.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1863..2675)
                     /gene="folK"
                     /locus_tag="VXM85_001638"
     CDS             complement(1863..2675)
                     /gene="folK"
                     /locus_tag="VXM85_001638"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1346186.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRNRGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2718..3272)
                     /gene="folE"
                     /locus_tag="VXM85_001639"
     CDS             complement(2718..3272)
                     /gene="folE"
                     /locus_tag="VXM85_001639"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1346187.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3253..4575)
                     /locus_tag="VXM85_001640"
     CDS             complement(3253..4575)
                     /locus_tag="VXM85_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1346188.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGMFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNVTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4577..5551)
                     /gene="folP"
                     /locus_tag="VXM85_001641"
     CDS             complement(4577..5551)
                     /gene="folP"
                     /locus_tag="VXM85_001641"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1346189.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVAIMFNPVMARPQHPSSLI
                     FPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5646..6353)
                     /locus_tag="VXM85_001642"
     CDS             complement(5646..6353)
                     /locus_tag="VXM85_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1346190.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLVLFIMGARKTKLASFNFSFFRAKDLARLVLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIIPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6407..7825)
                     /locus_tag="VXM85_001643"
     CDS             complement(6407..7825)
                     /locus_tag="VXM85_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1346191.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            8015..8827
                     /locus_tag="VXM85_001644"
     CDS             8015..8827
                     /locus_tag="VXM85_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1346192.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9166..10185
                     /gene="adhP"
                     /locus_tag="VXM85_001645"
     CDS             9166..10185
                     /gene="adhP"
                     /locus_tag="VXM85_001645"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1346193.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3008 a         2321 c         1988 g         2899 t
ORIGIN      
        1 actatcctcg atttgtccta ttcttatttc atattactat aaaaataagg taaacttttc
       61 aaaaaagcac gaaaaaagca cccatagggc aaacctttga gtgctttgaa acattgatat
      121 aatcgtattg attaacgttt tgaggcaact gaccttgtca ggttgcagcc acatttgtaa
      181 gcgactaaag tcgcaacaac tgtgtcaatt gcaccaattt agtaagaaag tataaaaaaa
      241 gaacaccccg aaaggtgctc tgtataagta tagtaattct ttcgaattaa cgtttactaa
      301 attgtgatgc tttacgagct ttcttaagac ctggtttctt acgttcaact ttacgtgagt
      361 cacgtgtaag aagtcctgcg cgtttcaatg aatcgcggaa gtctgggtct acttgaagaa
      421 gggcacgagc gataccgtga cggatagctc ctgattgacc agcgtatcca ccacctacaa
      481 cgttaacgaa aacgtcgtat gaacctacag ttgaagtaac tgcgaatggt tggttgatga
      541 caagacgaag gtcagcgtgt gggatatact cttcaacatc ttttttgtta acagtgattt
      601 taccagttcc tggaacaagg cgaacgcgtg caacagcgtt tttacgacgt ccagtacctg
      661 catattgtgc ttgtgacata ctttatttgt tcctttcctt agataagtcc tgaaatgtca
      721 agaacttctg gttgttgtgc agcatgagtg tgctcagctc caacaaatac tttcaacttc
      781 ataccttgag cgcgtccaag tgtattgtgt ggaagcatac ctttaactga tttctcaatc
      841 aaacgtactg catttttaga acgaagttca cctgcagaga tttgtttcaa tccacctggg
      901 tggtttgagt gagtgtagta gattttatca gttgcttttt taccagtcaa tttaactttt
      961 tcagcattga taacaatcac gaagtcacct gtatcagtgt gtggtgtaaa tgttggtttg
     1021 ttttttccgc gaagtacgct agcaactact gcagaaagac gtccaagtgg tacatcagtt
     1081 gcgtcaacta cgtaccattt acgttcaact tggcctggtt tagccataaa tgttgttttg
     1141 ttcatgattt ctcctatata tagttcgatt tttgtttacg gtgatgggtg ttccttccca
     1201 tcgaaaagta tttggaaggt tccggggcct ttcaaatggg gtaaacaata ccgcctacta
     1261 tcataccaaa tttcacccct aaaagtcaat agatataaaa aatatttttc ggtaacttct
     1321 caaaataact gaaagttata taatcaagtt aatttgggaa tttacctatt aacttcggta
     1381 gatttttggg aattttttcc ctatttctcc ctgtaactaa aatccttttc aaaaaatcac
     1441 cttttcttga tttttaatcc tatttgctgt atgataaggg aaaagaaagg ggacagagat
     1501 atggctttta ccaataccca catgcggtct gctagttttg gcattgttac cagcttgccc
     1561 gatgacatca ttgactcttt ttggtatatc atcgatcatt tcttaaaaaa tgtctttgaa
     1621 ttggaagaag aactcgagtt tcaattgctt aataaccaag gaaagattac cttccacttt
     1681 tcaagtcaac acctccctac agccattgat tttgacttta accatccttt cgaccctcgt
     1741 tatcccccaa gagtactggt tttagacatg gacggtagag aaactatcct cctcccagaa
     1801 gaaaatgacc tattttaaaa actctagcct tcagttgcaa gtaactgaaa actagagttt
     1861 ttctattttt tcaaagcatc atacaagttg cggatcggtt gttttaatat cggatggata
     1921 aaatgaggcg caatttcctg taaggactca aggacaaaaa ggcgttccgc tatgtaagga
     1981 tgaggcaata tgaggtcgtc tgtataaagg atctggtcct ccacaaagag caagtccaaa
     2041 tcaatcaaac gaggtcccca atgcacttct ctcacccgtc ccagctctga ctcaatggct
     2101 aacaaggttt ctaacaagtc ttgtgctggt agccaggttt ccacctcaac cacttgattg
     2161 gcaaagctat cctgctccac tccaccccaa ggctccgtcg ctaagacact ggactctttg
     2221 agaatatgga tacctcgatt tcgcattttc tcaatggctt gtttcaagtt tgcttgttta
     2281 tctcccatat tgcttcctag ggcgataaag gctcgttgct tgcggcgatg gatggttacc
     2341 gagcaagtat ctagtgacaa atgcaccggt gcccaaggtt ttttcagttc caacttcatt
     2401 tcttggacaa gaggataaga ctcaaaggta cgttccacca gtttataggc taccgtttca
     2461 atcaaatctt cactcgtttc ctgaaaccaa gtcgtccact gctgacacaa ttctccgtaa
     2521 tggacagagg ctgttaaatc caagtctgta gctgccttgg tcatatcata ggataggatg
     2581 gctgaaacga caaatttctg ccccaattct ttctcactag gaaaaagacc atgataggca
     2641 aacatttcca aatccttaat ctgcagttga tccataatga ttcctttcta gaaaaatccg
     2701 cttgaagcgg attcttttta tagccccatt aaacgataag cttggtcgcg gaggtcctta
     2761 tctgtttcaa atagaccacg agctactgtc gtcaaggttg cagtgcctgg ttttctaaca
     2821 ccacgcatac tcatacacat atgttccgcc tcaatgacaa caaaggctcc tttagcacct
     2881 agatagtcca tcaaggcatc ggccacttcg atattcaaac gttcttgaat ttgtggtttt
     2941 ttcgaataaa cttcaaccgt acgggctagc ttagacaagc ctgccacacg accatctgga
     3001 atgtaggcaa tgtgcgctct accataaaat ggcaagaagt ggtgttcaca catggtatgg
     3061 aaaaagatat ccttttctac caccatatta tcgtcaataa tttcaaagga ttttgacaaa
     3121 tgttcctctg ctgtttgacc aagacctgaa aaaatctctt gatacatacg ggctacacga
     3181 gcaggtgttt cctgcaagcc ctcgcgatta gcgttctctc ctacagcctc gataatcatt
     3241 tttacagctg cttcaatctt ttgtgtatcc attctcgttc ttcctctcca tcagataggc
     3301 cctcacttgg ctcaagaaat acaaggaacc tgtgacaatc ctaactgttt gtttctcttc
     3361 ttttttatct gtcacattct gctctagaaa atcatgccaa tcttggtagc tgagatttct
     3421 agacttagct gcctctttca gcacgctttc atccgtcgcc cgactatccg caaaatgtgt
     3481 tagagtaagc tcggtatctg gcatggctcc cagcaagtcc aacatatcct ccaaggcttt
     3541 ggttttgata caagtgaaga ggatttcctt atgataatcc gcaaaacgtt cttgcaaggt
     3601 taccaacaag gccttgatag catggggatt atgggctcca tccaaaatca tcaagggatc
     3661 tcttgacacg atttccaaac gccctggcca acttgtttct tccaaggctt gaccaagaaa
     3721 gtcattgctt gctagctctc gaccatcttc ttgacaaaaa gtatcaagta aagctatggc
     3781 catcccagca ttctctattt ggtacaaacc aagcaggcta gtctggaagc gaccttgtct
     3841 gacagcactt gtatagtcaa agacttcccc tgtcaccaca ctttcttgat gacgaacctg
     3901 ataatctgtc ccgtaggcaa gtctcggcgc atctttccct tccgcaatgc ggtcaatcac
     3961 agtcaaggct tctggagcaa tacgccctgt taccaagggc atcccttgtt tgataatacc
     4021 tgccttctgc tctgctatgg cctccaaggt gtcaccaagt agggccacat gatccaagcc
     4081 aatagttgta attcctgtca aaatgggctg acagacattg gtactatcca aaagtccacc
     4141 catgccaact tccatgatgg ccacatctac ttgctctgag gcaaagtagt cataggccag
     4201 ggctgtgata atctcaaact cggttgtgcc ctgtaaattg gcgaccgctt ctccctccag
     4261 caaagactga tagtctgcca tgagagcttc tagcctcgct tctgagatcg attccccatt
     4321 gatgctaatc tggtctgtgt aatgaatgag atagggcgag ctaaacatgc caactctcag
     4381 ccctagcttt tctagcatct ttttcaaaaa agcaatagtc gagcccttgc cgttagttcc
     4441 tccgatatgg aggaccttga gtttgagatg gggattgcca cgcaaagcta acagttccac
     4501 cattcgttcc aagccaaaat gcggttgatc cgtccggtag ttagcaatcc actgattgtt
     4561 ttcaatttct ttcatcttat ttatattgtt ttaaatctaa attttccgct tcatcagcca
     4621 gacgaatggc agaggcaatt tcaactgcca tcctgtgact agctacgtca tgcacgcgca
     4681 ccacttctac accctgtctc gcagcgatac tagttacatg agccgaagcc gtgtcccgat
     4741 ttcggaaacc aagctctgtc tcaggattga cttcaaaacc attctcctct aggatattga
     4801 tgacaaatcg cttgcgcgac actccgagaa agattggata gcccttctga tgtagtttat
     4861 ccaggtcccg taaaagaagc agattttctt tcttggtcag accaaagcca attcctggat
     4921 ccaacaggat attttctggt gcaataccag cttctgccgc tctcgctagt gctcgttcaa
     4981 agaaagcctc catcaattct tcgattggca atgtttcaaa gtcagctaac tcttcctctg
     5041 taaaagcttg accaaaacca aaatgaggga agatgagcga gctagggtgc tgaggtcgcg
     5101 ccataactgg gttaaacatg atggccactt gcgctctcgc ttcagctacc acatgagcca
     5161 ttttctcatc acccataaga ccagtgatat cattgactag atcggcacca gcagccaaag
     5221 cagcctctgc tacttgactc ttccaagtat caatagagat gaggacatca ctttccttgc
     5281 gaatcgcttt gatcactgga acaacacgct ggatttcctc ttctatctca acatagctac
     5341 tgcccggccg agtcgattct ccgccgatat cgagcatact ggctccttcg gctatcaatt
     5401 tacgagcctg ctggagtgcc tgctcaagag caaaaaattg accaccgtcc gaaaaggagt
     5461 ctggggttac attgataatt ccgcaaataa ctgtctttgc atgattggct ttacttgaca
     5521 tatcggtcac tccctcaagg attttcatca tattattttt ctattttacc ataaaaagaa
     5581 aaagatggac acgattgcat tcatctttcc ctagtagaaa gaggtcggtt cctacataaa
     5641 aacttttaca cagaaattcc taatgtccga ctcataatca ccacaagagc caacaaacag
     5701 aaagcaatcc cattaacaat catgtgaagc aagatcgaca tttccaaacg ttgggtcttg
     5761 taggctgtcc aagatagaac tgtcgacata cctccataaa tcaataaaga aggtaaatta
     5821 cttggttgat gcaataaagc aaacacaatc gtaccgacta caaatcccaa gttctccttg
     5881 cctcggaaaa tctttttagg aataatccca cgacacaaga tttcctcaca aatcggagca
     5941 agcaaggcta gcaagaagaa gctagaaatc aacgaactat tctgaaccat atcattaatc
     6001 tgagactggt tagctgttgt cgtctcattt gacagttgca acaagataga accaagtata
     6061 tttgacccga caataactag ataactcaag accaaacgtg ccaaatcttt agctctaaaa
     6121 aaagaaaaat taaaactagc taacttggtt ttacgagctc ccataataaa tagcaccaga
     6181 accacaattg aaaggccagc aactatcagc cctgactgta gcagggctac ttcttttaaa
     6241 gtcaaaatag aggtaaccac taggggaacc tgatataaaa ggacggcaag taaaaagacc
     6301 aaaatccagc ctgcacgatt caacaattct ttccacatat ttttctcttt catcacctat
     6361 ctccttttgt attaaaaaag gggggaaatc cccctgggtc attctattat aaggccatgc
     6421 tgatgtagtt aaggataaac aaggcatcca aaatccaaat catgacatga acatctttaa
     6481 cttgaccttt aacaagctta gtcaaagtgt aagtcaagaa accaactgca atcccttgag
     6541 tgatagagta gctgaatccc ataaagatag atgtgaagaa ggcaggaact gcttcagaca
     6601 tatcatccca atggatattt ttcaagctac caagcatcat aatcccaacg ataatcaaga
     6661 ttggagctgt agccgctgtt ggtacgatcg ctagaagtgg gctaaagaag cttgaaatcg
     6721 caaaacagat agctacaacc aaggctgtca aaccagtacg tccacctgca ccgataccag
     6781 cagcagactc aacataagtc gttacgtttg aagtacctgc aatggcaccg actgtcgttc
     6841 caatcaaatc agagtaaaga gccttatcca atttggctga ttggtgattt tcaccatttg
     6901 tcgctacgat accaactttt tcacctgtac cgatcaaggt accaattgtg tcaaaaatat
     6961 ctgtcaatga gaaggcaaga atggccatca gagtttcagg caagcgagct gtatctgaaa
     7021 ccaaagctcc caaaccttct gaaccaagag ctgcaccaaa gattgtcttc aaatcttcaa
     7081 aagctgcacc aacatggtta ttagcaaaat cgatactaga caaatcaacc aaaccaactg
     7141 caatagcaag aacagttgtt gtcaagatag agagaataat tcccccttta atccctttga
     7201 tgacaaagaa gatagtaatg gcaagtcctg caagagccac taaaacagct ggattattaa
     7261 agctgaccaa tcctggaact gctgaagagt ttgctgcaat cgttgcttga gctttgtcag
     7321 ccccttctcc tacaacagta tagttgcctg gatcaatcgt gaatttcaaa agtccagcat
     7381 tcttaatccc tacataggca aggaagacac cgataccagc tgaaatagct gagcgaagag
     7441 cattgggaat cgattcaatg atcattttac gaacatttgt caaggtaata atcaatgaaa
     7501 taatcccaca gatgaagacc atagctaggg cttcttgcca agaataacca agcccgaata
     7561 caactgtaaa ggtaaagaag gcattgagtc ccatacctgg cgcttgggca taaggtaagt
     7621 tagcataaaa agccatcatc aaggtacccg ctactgcacc aataatcgtc gctaggaaga
     7681 cgccctgagc aggcattcct gtttgtgaaa gtatttgtgg gtttacaaag agaatatagc
     7741 tcattgcaaa gaaagttgtt aaaccagcga gaacctctgt acgaacgtct gtaccgttct
     7801 cttttagttt aaataatttg tccatcatca atctcctttt aatttttacg aacaataata
     7861 gaattatatc atttattatt cactttttca atgtctttgt ttgatttgtt taagaaatta
     7921 atccaatttc attttttgtt tggaatctgc ttttctgcct gctttctgtt ataatagaag
     7981 acatcttatc tgaaaacaca ctagaaaggt ccatatgacg aaaaaaatta ttgcagttga
     8041 cctggatgga accctgctca actcagacag tcaaatttct gactttacca aaagaaccat
     8101 caaaaaagtt gctgaaaaag gccatcaggt tattattacg acaggtcgcc cttaccgtat
     8161 gtcaaaagat ttttaccgtg aactgggctt agacactcct atgattaact tcaacggatc
     8221 ccttactcat ttaccagacc aagtttggga ttttgaaaag tgtttgactg tagacaaaaa
     8281 atatctgcta gatatggttc aacgttcaga ggacattcaa gccgatttta tcgctggaga
     8341 atatcgtaaa aaattctaca ttacaaatcc caatgaagaa attgccaatc ccaaactatt
     8401 tggtgtagaa gctttccagc ctgaagatca attccagcct gaattggtga ccaaggaccc
     8461 taactgtatc ctcttgcaga ccagagccag tgacaaatat gccttggcaa aagacatgaa
     8521 cgccttctac cagcatcaac tttctatcaa tacctgggga ggtccgctca atatccttga
     8581 atgtacccca aaaggtgtca acaaggcctt tgctttggac tacttgctca agataatgaa
     8641 tcgtgacaaa aaagatttga ttgcctttgg agatgaacac aatgataccg aaatgctcgc
     8701 ttttgctggg aagggttatg ccatgaaaaa tgccaatcca gagctactcc cttatgcaga
     8761 tgagcaaatt tcccttacca acgaccaaga tggggttgcc aaaaccctac aagacttatt
     8821 cttataacct atactgatac tcaatgagaa tcaaagagca aactaggaag ctagccgtag
     8881 gttgctcaaa gcactgcttt gaggttgtag atgaagctga cgtggtttga agagattttc
     8941 gaagagtatg accagcttgc aagctggtct ttgtgctctt ttcacagtct catcaaccag
     9001 ttaatcgatt gttctacttt ttgtctccaa aaccttggaa aaggctttct tttgtgatat
     9061 acttcacata taaatacaag agagctcgtc acactcgact ctgttgacaa aaaatcaatc
     9121 cagtcccgtt gtccctgttc tcggtcaata tcaaggagga tagctatgaa agctgttgtt
     9181 gtaaatccag aaagcactgg tgttgctatt gaagaaaaag tactccgtcc acttgaaact
     9241 ggggaagcac ttgtagaagt tgaatactgt ggcgtttgcc acaccgacct ccacgttgct
     9301 catggtgact ttggtcaagt cccaggacgt gttcttgggc acgaaggtat cggtatcgtt
     9361 aaagagattg ctccagatgt gaaaagcctt aaagtcggtg accgcgtcag cgttgcttgg
     9421 ttctttgaag gatgtggcac ttgcgaatac tgtacaactg gtcgcgaaac cctttgccgt
     9481 acagtgaaaa atgctggcta ctcagtagac ggtggtatgg ctgaacaatg tatcgtaact
     9541 gctgactatg ctgtcaaagt tcctgacgga cttgatccag cccaagcttc ttctatcaca
     9601 tgtgctggag taacaaccta taaagctatc aaagaagcaa aagttgaacc aggccaatgg
     9661 gttgttcttt acggtgctgg tggacttggt aacctcgctg ttcaatacgc taaaaaagta
     9721 ttcaatgctc atgttatcgc agtcgatatc aacaatgaca aacttgccct tgcaaaagaa
     9781 gtaggcgctg acattgtgat taacggcctc gaagttgaag atgtagctgg actcattaaa
     9841 gaaaaaactg atggaggagc tcattcagct gtcgtaactg ctgtgtctaa agttgccttc
     9901 aaccaggctg ttgactccat tcgtgctggt ggtcgcgtcg tcgctgttgg tcttccttct
     9961 gaaatgatgg aactcagcat cgttaaaaca gtcctcgatg gaatccaagt catcggttct
    10021 cttgtcggaa ctcgtaaaga cttagaagaa gccttccaat ttggtgcaga aggtctggta
    10081 gtcccagttg ttcaaaaacg tccagtagaa gatgctgttg ccattttcga cgaaatggaa
    10141 aaaggccaaa tccaaggacg tatggtactc gacttcaccc actaatctaa tagaaaccta
    10201 ttttaaaagt tggaag
//
LOCUS       DAVLRT010000067         9897 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLRT010000067 DAVLRT010000000
VERSION     DAVLRT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.067"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            222..809
                     /locus_tag="VXM85_001646"
     CDS             222..809
                     /locus_tag="VXM85_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1346194.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            736..2379
                     /locus_tag="VXM85_001647"
     CDS             736..2379
                     /locus_tag="VXM85_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1346195.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFKPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILKAYELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQTQGFYHHLFNK"
     gene            2390..3751
                     /locus_tag="VXM85_001648"
     CDS             2390..3751
                     /locus_tag="VXM85_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1346196.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTSNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPIITDTREVLITYYVASDYLPLLDKGQTVRLKLEKIGNHGITIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            3808..3963
                     /gene="blpC"
                     /locus_tag="VXM85_001649"
     CDS             3808..3963
                     /gene="blpC"
                     /locus_tag="VXM85_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1346197.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDILYSLNIIKHNNTKG
                     LHHPIQL"
     gene            complement(4008..5348)
                     /locus_tag="VXM85_001650"
     CDS             complement(4008..5348)
                     /locus_tag="VXM85_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1346198.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGIGLTFERIFKAFLLKILL
                     AFVFVMIGYMVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQEQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRREILDTGFQK
                     SLTKINWIMGAYYLVIQSLSYFEYEQGIQSTTVRHLILVFYLLFFMGVIKKLDTYLKD
                     KLHDRLDQEQVLRYRDIERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYDLVLRDSSQKLQDNKYDLGRLVNVRDRALKSLLAGKFIKAREKDIVFNVEVPEEI
                     QVEGMRLLDFLTIVSILCDNAIEASAEAGQPHVSIAFLKSGAQETFIIENSIKEEGID
                     ISEIFSFGVSSKGEERGVGLYTVMKIVESYPNASLNTTCQDQVFRQVLTMIPTE"
     gene            complement(5362..6099)
                     /locus_tag="VXM85_001651"
     CDS             complement(5362..6099)
                     /locus_tag="VXM85_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1346199.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIREQDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(6104..6442)
                     /locus_tag="VXM85_001652"
     CDS             complement(6104..6442)
                     /locus_tag="VXM85_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1346200.1"
                     /translation="MKHMIIQTQKTVYKVNVDDVYYIQTHPTKAHTVKIVTEEASFNM
                     VQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6728..7057
                     /locus_tag="VXM85_001653"
     CDS             6728..7057
                     /locus_tag="VXM85_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1346201.1"
                     /translation="MTDTDPIKRAQTLITDLNKAYQACKQATADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7225..8274)
                     /locus_tag="VXM85_001654"
     CDS             complement(7225..8274)
                     /locus_tag="VXM85_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1346202.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVEVVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8271..9002)
                     /locus_tag="VXM85_001655"
     CDS             complement(8271..9002)
                     /locus_tag="VXM85_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346203.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9070..9480
                     /locus_tag="VXM85_001656"
     CDS             9070..9480
                     /locus_tag="VXM85_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1346204.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9490..9777
                     /locus_tag="VXM85_001657"
     CDS             9490..9777
                     /locus_tag="VXM85_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346205.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         3279 a         2110 c         1727 g         2781 t
ORIGIN      
        1 aaatttttca tccagatctt gaattgtcat cgaaacgtct tgaattagct tttttatttc
       61 aagccacctc tatttcaagc cacctctaaa tgtttaaaaa aaataatttc taatcacttt
      121 tttaccattc aggaagtttt aatgactatt caagatttca taaaatatga acttagtttt
      181 atgacataat agacctatcc actatatgaa aggaattgcc aatgacttct tataaacgta
      241 catttgttcc tcaaatagat gcgagagact gtggtgtcgc tgccttagcc tcgattgcta
      301 aattctatgg ttcagatttt tctctagctc acttgagaga acttgcaaag accaataaag
      361 aagggacgac tgctcttggc attgtaaaag ccgctgatga aatgggcttt gaaacaagac
      421 ctgttcaagc agataaaacg ctctttgaca tgagtgatgt cccctatcca tttatcgttc
      481 acgttaacaa agaaggaaaa ctccaacatt actatgttgt ctatcaaaca aagaaagact
      541 atctgattat tggtgatcct gacccttctg taaaaatcac taaaatgtca aaagaacgct
      601 ttttctatga atggactgga gtagctattt ttctagctac caaacccagc tatcaacccc
      661 ataaagataa aaagaatggt ctactaagca agcttccttc ctctgatttt caaacaaaaa
      721 tctctcattg cttacattgt tctctcaagc ttattggtca ctattatcaa tataggtggt
      781 tcttactatc tccaaggaat cttggatgaa tacattccaa atcagatgaa atcaacttta
      841 ggaatcatct cagttggtct ggttatcacc tatatcctcc aacaagtcat gagcttctcc
      901 agagattatc tcctaaccgt tctgagccag agattaagca ttgatgtgat tttatcctat
      961 attcgccata tttttgaact tcccatgtct ttctttgcga cacgtcgtac aggagaaatc
     1021 atttcacggt tcacagatgc taactctatt atagatgcct tggcttctac cattctttct
     1081 ctttttctgg atgtttctat tctgattctt gtagggggag tcttgctggc acaaaaccct
     1141 aatctcttcc tcctttctct tctttccatt cctatataca tgttcatcat cttttctttt
     1201 atgaaacctt ttgaaaaaat gaaccatgat gtcatgcaaa gtaattctat ggttagctct
     1261 gccattatcg aagatatcaa cgggattgaa actataaagt cgctcacgag tgaagaaaat
     1321 cgctatcaaa atatagacag cgaatttgta gattatttgg aaaaatcctt taagctcagt
     1381 aaatattcta ttttacaaac gagtttaaag cagggaacaa aattagttct gaatatcctt
     1441 atcctatggt ttggcgctca attagtcatg tcgagtaaaa tttctatcgg tcagctgatt
     1501 acctttaaca cacttttttc ttactttaca actcctatgg aaaatattat caacctccaa
     1561 accaaactcc aatctgcgaa ggtcgctaat aaccgtttga acgaagtcta tctagtcgaa
     1621 tctgaatttc aagttcaaga aaaccctgtt cattcacatt ttttgatggg cgatattgaa
     1681 tttgatgacc tttcttataa gtatggtttt ggacgagata ccttaacaga tattaatctc
     1741 acgattaaac aaggagataa ggttagccta gttggagtta gtggttctgg taaaacaact
     1801 ttagccaaaa tgattgtcaa tttctttaaa ccctacaaag ggcatatttc catcaatcat
     1861 caggatatta aaaacattga taaaaaagtc ttgcgccgtc atattaatta cctaccccaa
     1921 caagcctata tctttaatgg ctctattttg gaaaacttaa ccttgggcgg taatcatatg
     1981 attagccaag aagatattct aaaagcttat gaattagctg aaattcgtca agacattgaa
     2041 agaatgccta tgggctatca aactcagctc tctgatggag ctggtctatc aggaggacaa
     2101 aagcaacgaa tcgctctcgc tcgtgctctt ttaactaaat ctcctgtttt aatactagac
     2161 gaagctacta gcggtcttga tgtcttgact gagaaaaagg ttatagataa tcttatatcc
     2221 ctaactgata aaaccattct ctttgtagcc catcgtctca gtatagccga acgaactaac
     2281 cgtgtcattg ttcttgacca ggggaaaatc attgaagttg gtagtcacca agagttaatg
     2341 cagacgcaag gcttctacca tcatctattc aataaataag gagaatgtca tgaatcctaa
     2401 tctttttaga agcgtcgagt tttatcagag acgttaccat aactatgcga cagtattaat
     2461 catacctctg tcattactgt ttactttcat cttgattttc tcccttgttg ccacaaaaga
     2521 aattactgtt acttcccaag gggaaatcgc ccctacaagt gtcattgcat ctattcagtc
     2581 gaccagtgat aatcctatcc ttgctaatca tttagtggca aatcaagtag ttgaaaaagg
     2641 ggacttactc atcaaatact ctgaaacaat ggaagaaagt cagaaaactg ccttagcaac
     2701 tcaattacaa agacttgaga agcaaaaaga aggacttgga attttgaaac aaagcttaga
     2761 aaaagcgact gatctttttt ctggcgagga tgaatttggc taccataata cctttatgaa
     2821 ttttactaaa caatcccatg atattgaact gggtatcaca aagactaaca ctgaggtttc
     2881 aaatcaagct aatcttgcca atagcagttc atcagctatt gaacaagaaa ttacaaaagt
     2941 tcaacaacaa attggagaat atcaagagct gagagatgct atcataaata acagagcacg
     3001 cttaccaact agcaatccac accagtcaat tttgaatcgt tatcttgtag cctcacaagg
     3061 acaaacacaa ggaacggcag aggagccatt tttatctcaa attaatcaaa gtattgcagg
     3121 tcttgaatca tctatcgcaa gcctcaaaat tcagcaagct ggtatcggaa gtgtagcaac
     3181 ttatgataat agtttagcaa ccaaaattga agtactccgc actcaatttt tacaaacagc
     3241 ttctcagcaa caactaaccg tggagaacca attaacagaa ttaaaagtac aactagatca
     3301 agcgacacag cgcttagaaa acaatacctt aaccgctcca agtaaaggta tcgttcatct
     3361 gaacagcgaa tttgaaggta aaaatagaat tccaactggt acagaaattg ctcaaatatt
     3421 ccctatcatc acagatacaa gagaagtact aatcacttac tacgtagctt ctgactatct
     3481 acctctacta gataaaggac aaactgtaag attaaaactg gagaagattg gaaatcacgg
     3541 cattaccatc atcggccaac ttcagacaat tgatcaaact cctaccagaa cagagcaagg
     3601 caatctcttt aaattaaccg ctcttgcaaa actatctaat gaggatagta aactcatcca
     3661 atatggctta caaggtcgcg tcactagtgt aactgcaaag aaaacatatt ttgattattt
     3721 caaagataaa attttaaccc attctgatta attttcagat aacactctat aactatttat
     3781 tatcttatca aaaaggagaa tcataacatg gataagaaac aaaacttaac ttcatttcaa
     3841 gaactaacaa ctaccgaact caaccaaatt acaggtggag gactgtggga agatatctta
     3901 tacagcctta atataattaa gcataacaat acgaaaggac ttcatcatcc aatacaacta
     3961 taaaaaataa gaccgagtaa caagaactct cggtcttatt tttttcatca ttctgtaggt
     4021 atcatagtaa gcacctgacg aaagacttga tcttgacagg tagtatttaa actggcattg
     4081 ggataacttt ccacaatctt cataacggta tagagaccga cacctctctc ctccccttta
     4141 gaactaactc caaaggagaa gatttcagaa atatcgatgc cctcttcttt gatggagttt
     4201 tcaatgataa aagtctcctg tgctccactt tttaaaaagg caattgaaac atgaggttga
     4261 ccggcctctg cactcgcttc aatagcatta tcacaaagga tagacacaat ggttagaaaa
     4321 tcaagcagtc tcatcccctc aacctgaatc tcttcaggaa cttcgacatt aaagacaatg
     4381 tccttttctc tagcttttat aaattttcca gctaggagac ttttgagggc acgatcccga
     4441 acattcacca atctgcccag gtcatattta ttgtcctgca atttctgact ggaatccctt
     4501 aagaccaagt catagacctc ttttatctgc tccatatcct cctcttcaat acccagacgt
     4561 aaacttgtca agaggttggt gtagtcatgg cgaaaactcc ttacttcctt gtaaagttcc
     4621 tctatatgcc gactatagcg ttctatatct ctatagcgca agacctgctc ttggtccagt
     4681 ctatcatgga gtttgtcctt caaataggta tccaatttct tgataacccc cataaaaaat
     4741 aggaggtaaa acactagaat gagatggcga acagtcgttg attgaatacc ttgctcatat
     4801 tcaaagtaag acagactttg tatcaccaaa tagtaagccc ccattatcca gttaatctta
     4861 gtcagggact tttgaaaacc tgtatctaga atctcccttc tcaaactagt aaaatcatag
     4921 tccaaccatt tcaaaaaggc tagagaaatg aagaaattga aaattattat acataaccca
     4981 gtaaatgagt agccatcata tacttgctct tgtcccaaaa atggaagcac aaaataggag
     5041 actcctctat aaaagagatt caccaatatc attggaaaga gaccataaaa gaaaaggagt
     5101 tttttaggaa gccctcttaa caataagaaa gacaagccta tgccgtacaa gggttccata
     5161 aaataagata ggtaaacatt tcctaccata tagccaatca ttacaaaaac aaaggccaac
     5221 agtatcttca aaagaaaggc cttaaaaatc ctctcaaaag tgagaccaat tccatctacc
     5281 ttaaagaaaa tgacaatttt taatccgtga gtaacaagtg tatacaacaa tatccaagca
     5341 acattcataa actctcctag ctcagtgtaa gttattaata gcctcagaca cttccctgac
     5401 cttataacgc gcgatcagac agcttccacc attgggaaag aaaaggagtt tttctttctt
     5461 atccaaatgc accacatttg caggattgat gagaaaagag cgatggcact gcaagagacg
     5521 gggttcctgc ttaaaaacct cctctaaact cgccgtaaat tctagcctgt ccgtcttggt
     5581 atagagaata acacgatggg gtcttgggga tgtttcgaga tagtaaacct ctttgaaagg
     5641 atattggaat tgggcaaatt ttgatttaaa gtaaaagcaa tcttccgcca gacttttact
     5701 atcttgacta ttggcataga ggagggctgt ctcgatacga gattcaaact cctctgccga
     5761 aagggcctta tcaatgtagt ccaaagctga cacttggtag cgaaaggaca gaggcataaa
     5821 ctccgagtga gtcgtcacaa agacgattag ggcataaggg tcttgttccc gaatctttct
     5881 agctacttcc agtcccttca tctcttcatt tcgaatctca atatccaaaa agaatagctg
     5941 atgggccccc ttctcatgca cctctgccag cagttggtcc ggcttgccaa atacctcaaa
     6001 agagctagga atgatatgat gtgctttcaa aagtttctca atcgtcgttt caattctagt
     6061 ctgttgggaa aaatcatctt ctaaaacaaa tattctcatc ttcttactct ccctctttca
     6121 accatttttg acgaatttct ctatagcgac gtctggcata cttgacagcg tattgacctt
     6181 cttctccaag aaaaaggatt ctttcttgaa aatcaatcga ttttacttta tcaagattga
     6241 ccaaacaatt tcgatgacat ctcatcaagg tttccccata ttggttctca agattactta
     6301 aattttgaac catattaaaa ctagcctctt ctgtaacaat ctttacagta tgggctttag
     6361 ttggatgtgt ttggatatag tagacatcgt cgacgtttac tttatagact gttttctgtg
     6421 tctgaataat catatgtttc attggattct cctcctaaga aaagaataac agaaaaaatc
     6481 taacacaagc acacccaatc ctaaacagtc tttgaaagct cctaaaagaa ttatttttct
     6541 acaatatcat gatgagctta ccactatttt atcataaaat tcactcagcg caattcaaga
     6601 catttcaatg acaattaaag atttaaaggc gaaaaaggcg aaaaaggaga taaaagtgat
     6661 agactgacag tatcaaaaca cagttgctag aatcaagact ggcacccaga ttagaggagg
     6721 aattctcatg acagatacag accctatcaa aagagctcag actttgatta ctgacttaaa
     6781 caaagcctat caagcatgca aacaggcaac cgctgacgac gtccgctttc aggagcaatt
     6841 aaactctatt cttggttttc tagccaaggc tgaaacagtg gataatcgat tcttgattga
     6901 attggaaaaa ttttaccaga cttccagtct tctcatggga ctcagcgctc ttgatccaga
     6961 tgctccaact cgcgccgctt ggcgggccta tgaccgcttc cactttgacc aagtcaagac
     7021 caagttaata ctcaatgaga atcaaagagc aaactaggaa gctagccgca agctgtactt
     7081 gagtacggta agacgacgct gacgtggttt gaagagattt tcgaagagta taagtctcta
     7141 cggaccaacc attatcctgt agtaacaaaa aagaagttga gacagactga actcaacttc
     7201 ttttttagtg tcgtatcagc aatgttagtc ctgcatctga cgttttacct gataaggcaa
     7261 atacaagact agtaaaacca aaccagctcc tagtaaggct agaagggcta gtttttcttg
     7321 gaaggtaatc aacccaacaa ctacttccac aagtaaaaca aaactggtca atcctcgaac
     7381 tacctcctgt aagccttttt ccttttgccc cttgtccagc ggaaagagtt gggtcaaata
     7441 ctggtagtca aaggcatgat agagggccag caactggaag agcaagaggt agttaaagag
     7501 aactaccact gctgtcgcaa tccaagcttg ctcgataaaa acctgcgcca gtacggaaag
     7561 taagagaaga cgaagactga gagcaaagag gtcgccattt cgcagataag aacgcagata
     7621 gagattttgc caaatcttcc caggcacctt ctgaacagcc tttaagataa agtccagata
     7681 ggcacgacgc ttgacactgt ttgaaattcc cttgacctgc gtaaagaggg caaagaaacg
     7741 aagcaagact tgcttacgct tgctttcttg agaaataaga tagtcccagt ccagtccagt
     7801 ttcagtgaaa aatttgctgg ccttttgacg aaagtggaaa tattttccta cccccaataa
     7861 aagcacatag agcagaaaaa ctggcaagcc ataacccatt gctaaaaaca agggcgcaaa
     7921 taacagcaag aaaagggtct gtacaaagag ccaaaagact agggaaatgc cagtttgacg
     7981 cttgagatgg agcttgattt cctcttctcc aactaagaga aagagcttgt ctggagcctc
     8041 catataggtg gcaattcctc cccaaagtaa aagtaaaaca gacgtaattc ctacaaacaa
     8101 aaggataggc caatgatttt caggaaaatg ttgtaagagt tgactgtact ggtaggctag
     8161 aaagcccaac aggacaagca ggaacaagac aaagtggtca ttgagcacat agcgcagata
     8221 accaagacac tccttacgaa aggcctgctt tctctttaaa aacaagtctt tcatagatcc
     8281 tcctctttgg tcagagccaa gtaaatatca ttcaaactag cctcaggcat atcaaaggct
     8341 tcacgtagtt gcaggagatt gcctttggca cgcacctctc ccttgtgaag aatgacaaag
     8401 gcatcacaca tcttctccgc cgaatccagc acgtgggtac tcatgagaat agacttgccc
     8461 ttttgcttct ccacttccaa aagctgaatc aaatcagaaa tagccagcgg atcaagacca
     8521 aggaaaggct catccacgat gaaaagactt ggatccacca caaaagcaca gataatcatg
     8581 accttctgct tcatcccttt tgaaaaatga acagggaacc agtctaattt ctgttccaaa
     8641 cggaacattt ttaacaaggg ctctactcgt tcgaaagcca ctttttgctc aataccgtaa
     8701 gccatagcaa ccgtttcgat atgctctctg agggtcaatt cctcatacag actaggcgtc
     8761 tcaggaatgt agccaatctg cttgcggtag ctagtcgcat ctccttgcag agtcaggcca
     8821 ttgatattga tggagccact ataaggtgcc aacagaccga taatctcatt gatcgtcgtt
     8881 gatttcccag caccattgag accaatcaaa ccgaccaact gcccactttc aacagtaaag
     8941 gacacatctt tcaaaacagg aacatgaaca tagccacctg tcaggttttt aatttctaac
     9001 atattttctc caaatctggt ataatgtagc tatattatat caaaattcag tacagtagag
     9061 gtagatttta tgtcagattg cattttttgt aaaatcatcg caggggaaat tcctgcttca
     9121 aaagtatatg aagatgagca ggtccttgcc tttcttgata tctctcaagt aacactagga
     9181 cacaccttgg tcgtgccaaa agaacactat cgcaatcttt tggagatgga tgctacgagc
     9241 gccagccaac tctttgccca agtaccaaaa gtagctcaaa aagtcatgaa agtcactaag
     9301 gctgctggta tgaatatcat ttccaactgt gaagaagtcg ctggtcaaac agtttttcat
     9361 actcacgttc accttgtgcc tcgctacagt gctgacgatg acctcaagat tgattttatc
     9421 gcccacgaac cagactttga caaacttgct caagtcgctg aaaccatcaa aaacgcttaa
     9481 ggagttgcta tgaaattatc caacctactg ctatttgcag gagctgcagc cggaagttat
     9541 ctggttacaa aaaatcgcca aaccatcaca gatgaagtct tgaataccac tgaccgcgtt
     9601 caagctatca aggacgatgt ggatattatc caaaacagcc tgcaaatcat taaccagcaa
     9661 aaagaactta tcaaggaata ccaagaagac ttgacttaca agtttaaggt cttggaaaag
     9721 gatatccaga ctagactagc tgtgataaaa gaaatgcagg gaactgaaga taagtaaaaa
     9781 gagccccccg gctctttttt aaaacgtaaa caagttatag tgaattgaat ttagaatagc
     9841 acaccatagt ttctaaagca ttgttagaga ttactttaaa atcctcttat caatttg
//
LOCUS       DAVLRT010000068         9776 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLRT010000068 DAVLRT010000000
VERSION     DAVLRT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.068"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(74..271)
                     /locus_tag="VXM85_001658"
     CDS             complement(74..271)
                     /locus_tag="VXM85_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1346206.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(346..1122)
                     /locus_tag="VXM85_001659"
     CDS             complement(346..1122)
                     /locus_tag="VXM85_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1346207.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            1244..1990
                     /locus_tag="VXM85_001660"
     CDS             1244..1990
                     /locus_tag="VXM85_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1346208.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2003..2983
                     /locus_tag="VXM85_001661"
     CDS             2003..2983
                     /locus_tag="VXM85_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1346209.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3178..3498
                     /locus_tag="VXM85_001662"
     CDS             3178..3498
                     /locus_tag="VXM85_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1346210.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3544..3852
                     /locus_tag="VXM85_001663"
     CDS             3544..3852
                     /locus_tag="VXM85_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1346211.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3869..5167
                     /locus_tag="VXM85_001664"
     CDS             3869..5167
                     /locus_tag="VXM85_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1346212.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            5318..7756
                     /locus_tag="VXM85_001665"
     CDS             5318..7756
                     /locus_tag="VXM85_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1346213.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPVTTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7775..8443
                     /locus_tag="VXM85_001666"
     CDS             7775..8443
                     /locus_tag="VXM85_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1346214.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            8461..9549
                     /locus_tag="VXM85_001667"
     CDS             8461..9549
                     /locus_tag="VXM85_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1346215.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTIKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         3235 a         1357 c         1961 g         3223 t
ORIGIN      
        1 cctgatagag atgttcacat cttatttcaa ttcactatat tagagtaaaa ttctctacaa
       61 aaagaagaat agcctatttt actattcttc cgagtgattt caatttcttt ggggaaatat
      121 ggagatactt tttaaatact ttggcaaaat atttatagtc tgaaaatcca acttggtcag
      181 agatagcgct taaaggggag ttgggagatt cagccatctt atctatagct tgtttcaggc
      241 gatattgtaa gatatattca tttagagtca tggggaagtc ttctttgata accttatata
      301 gatgcttgtt atctgcaaat agctagagca tttcaaggaa atgaactaga aataacaatt
      361 aatatggtgg ttcagaaata ccttttgata atcaataaga tcttcaggat gaagtgcgtt
      421 gactccatcc atttcatatt tccgatttaa taaacgatat ttgttttcac caaattgatg
      481 aaaagggagt agttgaactt ggtcgatatt taatgagtta aatagagtag cgaatttttc
      541 tgcatcctct aaactattgt taaaattagg aataactggg attcttaaaa cgatagtttt
      601 attttgagaa aaagcataat gaatgttttt aataatcatt tgattaaaaa ccccagtcac
      661 ttttttatgt tttatagaat tataatgttt taggtctgtg tagataaaat ccacatattg
      721 aattaaatcg ataaattttt catgatcaac aaaggcagta gtttcaatgg cagtgtgtat
      781 gtgatgttct ttagctgatt ttaagatggc tttagcaaat tcaaactgag caaatatttc
      841 acctcctgat aaagttaaac ctccaccgga ttcttcgtaa aattctttgt cttttaatac
      901 ctctgtaata atttcttcta cagtcttttc ttcaccgact aaggtgaatt tctctcgttg
      961 agcatctttc attttttcag gtttcattct ttgagattca ggattagaac accatggaca
     1021 gcgcagagga catcctttta aaaaaacagt tgtacgaata cccgggccgt catgaattga
     1081 aaagtgttga atattaaaaa taattccttt agatatttcc ataagagttc ctcctcttca
     1141 taagtctatc ctatcacaaa aacgaaagaa aaacaattac gaacgaaagg ttgatttgtt
     1201 gttttttgct ttttattttg gtagaataaa acggaggtgt aaaatgaaaa gattggaaca
     1261 aattattaaa ttagtatcag aatatgaaaa gatcgacgtt aatacattat cggaaaaatt
     1321 aaatgtatcg aaagtaacga ttagaaaaga tttagataaa ttagagtcaa aaggtttatt
     1381 acacagagag catggatatg ctgtattaaa tagtggagat gacttaaatg tacgtttgtc
     1441 aattaattat gaaattaaga gaaaaattgt tcaggaagca gtaaaattgg tgtcagataa
     1501 tgaaacaata atgatagaat ctggatcgac ctgtgcttta cttgctgagg aaatttgcaa
     1561 gcaaaaaaga aatgttacga ttgtaacaaa ttcatttttt atagcaaatt ttgtgagagc
     1621 ttatgattca tgtcgtatta ttgttcttgg tggagagttt cagaaggatt cacaggttac
     1681 tgtgggacct ttattaaaag aaatgataca gacttttcat gtgcatcaag cttttgttgg
     1741 gacagatggt tacgataaag agatgggctt tacaggaaaa gatttaatgc gcagtgaggt
     1801 agttcaatat atttcagcag cgtcggataa agtcattgta ctaactgact caagtaaatt
     1861 tgataaaagg ggtacagtaa gaagatttgc tttaagtcaa gtctatgaag taataacaga
     1921 cgaaaaactt tctaaacaaa atatagctac attagaaaat gctgggataa tggttaaggt
     1981 agtttcgtaa gaggttaagt gtatgaatca agataggaat aaactgctct ctaaaattgc
     2041 ttatctgtat tatattgaaa acttaaatca gtcacaaata gcagcaaaat taggaattta
     2101 tagaacctct attagtagaa tgttaacaga agcaaggaat gcaggaattg ttaaaattga
     2161 aatagagaat tttgatacaa atatgtttaa gttggaaaat tatgtaaaag aaaaatatgg
     2221 tctggaaagt ttagaaatta ttccaaatga atttgatgat aatccaacaa ttttatctga
     2281 aagaatttct caagttgcag caggtgtcct taggaattta attgatgata atatgaaaat
     2341 tggcttttct tgggggaaaa gtttaagtaa tttagtagat ttaattcaca gtaaaagtgt
     2401 ccgaaatgtt cacttctatc ctctagcagg tggtcctagt cacatacacg ctaaatacca
     2461 tgtgaataca ctgatttatg aaatgtctag aaaatttcat ggagagtgta catttatgaa
     2521 tgcaacgatt gtgcaagaaa ataaattgtt agcagatggt attttgcaat caagatattt
     2581 tgaaaatttg aaaaatagtt ggaaagattt agatatagct gtagtcggaa ttggtgattt
     2641 tagcaataaa ggaaaacatc aatggttaga catgcttaca gaggatgact ttaaagaatt
     2701 gaccaaagtt aaaactgtag gagaaatttg ttgtcgattt tttgattcaa aaggtaaaga
     2761 agtctatgaa aacttacagg aaagaacgat agcaatctct ttggaagatt taaaaaatat
     2821 tcctcaaagt ttagctgttg cttacggtga tacgaaagta tcttcgattc tttctgtctt
     2881 gcgtgctaat ttagtaaatc atttgattac agacaaaaat acaattttaa aagttttgga
     2941 agaagatggg gatttgactt ttagagagat tctaggtgag tgaaaatgat agactgattc
     3001 agtttatcgt ttttcttttt agttgattgc acatttgtgc ttatataaac aaaaatagtt
     3061 tatctgttgt ttttggattg acaactttat tatgtagttg tattctatag ttacaaaaga
     3121 aaattttaaa atttcaaatg aaaaaagctt tttacatagt gaaatgagga ggaatttatg
     3181 gaaattattg ttccagatca aattatcatg ggtttaattt tatatgctgg cgatgcgaaa
     3241 caacatattt ataaagcgtt agattacata aaaaatggta catgtgaacg atgtgaagaa
     3301 gaaatccagt tagctgatgc agccttatta gaagctcata atctacaaac aaaattcttg
     3361 gcacaggaag cgtctggtac aaagacagaa attacagctc tctttgttca ttcacaagat
     3421 catctcatga ctagtatgac ggagattaat ttaatcaaag aaattattag tttgagaaaa
     3481 gaacttcata aaaaataata ctagagtatt atcattgtta ttaacataga ggaggaaaac
     3541 ataatggtga agattggttt gttttgtgca gcaggttttt ctactggtat gcttgtaaat
     3601 aatatgaaaa ttgcagcgca agctagtgga gttgaggcag aaatagaggc gttttctcag
     3661 tctaaattag cggattatgc gccaaatata gatgttgcac tattgggtcc acaagttgct
     3721 tatacattag ataaatcaaa agaaatttgt gataagtgtg atgttccgat agctgttatt
     3781 ccgatgatgg actatggtat gttagatggg aaaaaagtat tagatttggc cctatctttg
     3841 attagtgggt aagaaaagga gatttattat ggcaaagatg gatgttcaga aaatcattgc
     3901 accgatgatg aagtttgtga atatgcgtgg cattatagct ctaaaagatg ggatgttagc
     3961 aattttgcca ttgacagtag ttggtagttt attcttgatt atgggacaat tgccgttcga
     4021 aggattaaat aagagcattg ctagtgtttt tggagctaat tggacagagc cgtttatgca
     4081 agtatattca ggaacttttg ctattatggg tctaatttct tgtttttcaa ttgcctattc
     4141 ttatgctaag aatagcggag tagaggcttt accagctgga gttctatctg tatctgcatt
     4201 ctttattttg ctaagatcat cttatatccc taaacaaggt gaggcaattg gggatgctat
     4261 tagtaaagtt tggtttggag gtcaaggaat tatcggtgct atcattatag gtttggtagt
     4321 aggaagtatt tataccttct ttataaagag aaaaattgtt attaagatgc cagaacaagt
     4381 tccacaagct attgccaaac agtttgaagc aatgattcca gcatttgtaa ttttcttact
     4441 ttctatgatt gtatatattt tagcgaagtc attgactaat ggcggaacat tcatagaaat
     4501 gatttattct gctattcaag ttccattgca aggtttaact ggatctttgt atggtgctat
     4561 tggaattgca ttctttatat catttttgtg gtggtttggt gttcatggac aatcggtagt
     4621 aaatggagta gtgacagctc tgcttttatc taatcttgat gctaataaag ctatgttagc
     4681 ctctggtaat ctatcattag aaaatggtgc acatattgtt actcaacaat ttttagattc
     4741 atttttaatt ctatcaggtt cagggattac gtttggtctt gtagttgcca tgctttttgc
     4801 agcaaaatca aaacaatacc aagccttagg aaaagttgcc gcttttccag caatatttaa
     4861 cgtaaatgag ccagttgtat ttggatttcc gattgtcatg aatccagtta tgtttgtacc
     4921 tttcattctt gttcctgtac ttgcagctgt gatagtatat ggagctattg caacaggttt
     4981 catgcagcca ttctcagggg taacattgcc ttggagtaca ccagctattt tatcaggatt
     5041 tttggtgggt ggatggcaag gagttattac tcagctggtg atattagcga tgtctacatt
     5101 ggtttatttt ccattcttta aagtacagga tcgtttagct taccaaaatg aaatcaaaca
     5161 atcttagagg tatttgtgtg ttactgttaa actcacacat ttgtgctaaa aattagagag
     5221 ttaaaatttt tctagttaaa agcttgaaaa tttctataaa aatcggtatt atattttcga
     5281 aagaaataaa aatattttcg aaagaaaggt ggttacgatg gtaaatacag aagtagcaag
     5341 aacaacaatc aagacagaat attttggcag ccttactgaa aggatgaaca aatatcgaga
     5401 agatgtttta aataaaaaac cttatattga tgctgagaga gcagttctag caacacgcgc
     5461 ctatgaacga tacaaggaac aacctaatgt cctaaaacgt gcatatatgc tgaaagaaat
     5521 tttggaaaat atgactatct atattgaaga agaatctatg attgggggaa atcaagcttc
     5581 ttccaataaa gatgctccta tttttccgga atatacgcta gaatttgttc tcaatgagtt
     5641 ggatcttttt gaaaagcgtg atggagatgt tttctatatt acagaagaaa caaaagaaca
     5701 acttagaagt attgctccgt tttgggaaaa taataattta cgtgctagag ctggtgcctt
     5761 attacctgaa gaagtgtctg tttatatgga aacaggattc ttcggtatgg aaggtaagat
     5821 gaattctgga gatgctcacc tagcagttaa ctatcagaaa cttttgcaat ttggcttaag
     5881 aggttttgaa gagcgggctc gtaaagcaaa agcagctcta gatttaacag atccagcaag
     5941 tattgataaa tatcattttt acgactctat atttatcgta atcgatgcta ttaaagtata
     6001 tgcaaagcgc tttgttgctc ttgctaaaag tttagccgaa aatgcaaatc ctaaacgtaa
     6061 gaaagaatta cttgagattg cagatatttg ctctagagtc ccatatgaac cggtaactac
     6121 ttttgcagaa gctattcaat cagtttggtt tattcaatgt attttacaaa ttgaatctaa
     6181 tggccactct ctttcatatg gccgttttga tcaatatatg tatccatata tgaaggctga
     6241 tttagaaagt ggtaaagaaa cagaagatag cattgttgaa cgtctgacaa atctttggat
     6301 taagacaatt acaattaata aggttcgcag tcaatcacat acattttctt cagcaggaag
     6361 tcctttatat caaaatgtta caattggtgg acagactcga gataagaagg atgctgttaa
     6421 cccattatct tatttggtat taaaatcagt tgcacaaacc catctaccgc aacctaatct
     6481 aactgtacgt taccatgcag gtttagatgc tcgtttcatg aatgagtgta ttgaagtgat
     6541 gaaacttggt tttggtatgc ctgcatttaa taatgatgag attattattc cttcttttat
     6601 tgcaaaagga gtattggaag atgatgctta tgattacagt gccattggat gtgttgaaac
     6661 ggcagttcca gggaaatggg gctatcgttg cacaggtatg agttatatga acttccctaa
     6721 ggttctactt atcacgatga atgatggaat tgatccggct tcgggtaaac ggtttgcacc
     6781 aagctttggt cattttaagg atatgaagaa cttttctgaa ttagaaaatg cttgggataa
     6841 aacactaaga tatttgacac gaatgagtgt tattgttgaa aattctattg atttatcatt
     6901 ggaacgagaa gttcctgata ttctatgttc agcattgact gatgattgta ttggtcgtgg
     6961 aaaacacctt aaagaaggtg gagcagtata tgattatata tcaggattgc aagttggaat
     7021 tgcaaatttg tcggattcat tagctgcaat taaaaaattg gtgtttgagg aagaacgtat
     7081 aagcccaagt cagctttggc atgcactgga aacagattat gccggagaag aaggtaaggt
     7141 cattcaagaa atgttgattc atgatgcacc taagtatggt aatgatgatg attatgctga
     7201 caaattggtt actgctgctt atgacattta tgttgatgaa attgctaaat atcctaatac
     7261 acgttatgga agaggtccaa ttggaggaat tcgttattca ggaacatctt ctatctcagc
     7321 caacgtaggg cagggacgtg gaacattagc aactccagat ggacgcaacg cgggtacacc
     7381 gttagcagag ggttgttcac catcacataa tatggatcaa cacggcccta catctgtttt
     7441 aaaatctgtt tcaaaattac caacagatga aatcgtaggt ggggttctct taaatcagaa
     7501 agtaaatcct caaacgttag ccaaagaaga agataaatta aaactaattg ctttgttacg
     7561 aacattcttt aatcgtttac atgggtacca tattcaatac aatgttgttt ccagagagac
     7621 gctgattgac gctcagaaac atcctgaaaa acacagagac ttaattgttc gtgttgcagg
     7681 atactctgca ttcttcaatg ttctttctaa ggcaacccaa gatgacatta taggacgtac
     7741 tgagcatact ttgtaaaata aagaggttct ttttatggaa tttatgcttg acacattaaa
     7801 tttagatgag attaaaaagt ggtctgaaat tttgccgcta gctggggtaa cttcaaatcc
     7861 cactattgca aaaagagagg gttctattaa tttttttgaa cgaatcaaag atgtaagaga
     7921 attgattggc tctacaccct ctattcatgt tcaggtgatt tctcaagatt ttgaaggcat
     7981 cttaaaggat gctcataaaa ttcgaagaca agcaggagat gatatattta tcaaagtacc
     8041 tgttactcca gctggattac gtgcaataaa ggcgctaaaa aaagagggct accatatcac
     8101 tgcaacagct atttatacag ttattcaggg attattagct atcgaagcag gagcggatta
     8161 cctagctcca tattataata gaatggaaaa tctgaacatt gattcaaatt ctgtcattcg
     8221 tcaattagct cttgctattg atagacagaa ctctcctagt aagattttag ctgcatcctt
     8281 taaaaatgta gcacaagtaa ataatgcttt agctgcaggt gcgcatgctg ttacagcagg
     8341 agcggatgtt tttgaatcag ctttcgccat gccatctatc caaaaggcgg ttgatgattt
     8401 ttctgacgat tggtttgtta ttcaaaatag tcgttccatt tagatagaga ggaaatacat
     8461 atgagaattt ttgctagtcc ttctagatat attcaggggg aaaatgcctt gtttgaaaat
     8521 gccaaatcaa ttttggattt gggaaattgc cctattctat tatgcgatca gttggtttat
     8581 gatattgttg gaaaacgatt tgaagattat ctacataggt atggtttcca tattgttctg
     8641 gcgctattta atggtgaagc ttctgacaat gaaatcaatc gagttgttgc cttggctgag
     8701 aaagaaaatt gtgatagtat tatcggtctt ggtgggggaa agacgattga tagcgcaaaa
     8761 gctattgcag atttgattga aaagcctgtt attattgctc caacaattgc atcgaccgac
     8821 gcacctgtat ctgctttatc tgttatttat acagatgaag gtgcatttga tcattatcta
     8881 ttttattcta aaaatccaga tttagttttg gttgatacaa aagttatttc acaagcccct
     8941 aagcgtttat tagcgtctgg tattgcagat ggtttagcaa cttgggttga ggcgcgtgcg
     9001 gttatgcagg caaatggaaa aactatgttg ggacaacagc aaacattggc tggagttgca
     9061 attgcgaaga aatgtgaaga aacgctgttt gcagatggtt tacaggctat ggcagcttgt
     9121 gaagctaaag tggtgacacc agcattagaa aatattgttg aagctaatac tttattgagt
     9181 ggtctaggtt ttgaaagtgg aggattagct gcggcgcatg caattcataa tggttttact
     9241 gcattgacag gtgacattca tcatttaaca catggtgaaa aagtagctta tggaacttta
     9301 gtacaactat tattggaaaa tagacctaaa gaagaacttg ataagtatat tgagttttac
     9361 aaaaaaattg gtatgccaac aactataaaa gaaatgcatt tggatcaagt tggatatgat
     9421 gatttaataa aagttggtaa acaagcaact atggagggtg agacaattca tcagatgccg
     9481 tttaagattt cgccttcaga tgttgctcaa gctattatcg ctgtagatgc ctatgtaaat
     9541 tcaaaataaa caataaggac tactgttttc caaatggtag tcttttattg atcctcgtat
     9601 tgaattctat agcagattga aataagatga gaacaaatcg attgggaaag taaaattaat
     9661 ttctataagt gttttagcaa ttgtttcgta ctatttcaga ttcagtctac tatacgttct
     9721 tcataaatca aaaagcgaca taggttgtcg gctatttatt gtgaatacat taatta
//
LOCUS       DAVLRT010000069         9708 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLRT010000069 DAVLRT010000000
VERSION     DAVLRT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.069"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(162..1925)
                     /locus_tag="VXM85_001668"
     CDS             complement(162..1925)
                     /locus_tag="VXM85_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1346216.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            complement(1934..2524)
                     /locus_tag="VXM85_001669"
     CDS             complement(1934..2524)
                     /locus_tag="VXM85_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1346217.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            complement(2622..3113)
                     /gene="rocS"
                     /locus_tag="VXM85_001670"
     CDS             complement(2622..3113)
                     /gene="rocS"
                     /locus_tag="VXM85_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1346218.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            complement(3190..3882)
                     /locus_tag="VXM85_001671"
     CDS             complement(3190..3882)
                     /locus_tag="VXM85_001671"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1346219.1"
                     /translation="MKIGIIAAMPEELAHLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            complement(3899..4213)
                     /locus_tag="VXM85_001672"
     CDS             complement(3899..4213)
                     /locus_tag="VXM85_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1346220.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            complement(4225..4770)
                     /locus_tag="VXM85_001673"
     CDS             complement(4225..4770)
                     /locus_tag="VXM85_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1346221.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            complement(4780..6159)
                     /gene="glmU"
                     /locus_tag="VXM85_001674"
     CDS             complement(4780..6159)
                     /gene="glmU"
                     /locus_tag="VXM85_001674"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1346222.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRCRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPRNQ"
     gene            6293..7093
                     /locus_tag="VXM85_001675"
     CDS             6293..7093
                     /locus_tag="VXM85_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1346223.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSIWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            7188..8030
                     /locus_tag="VXM85_001676"
     CDS             7188..8030
                     /locus_tag="VXM85_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1346224.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYSLNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNTGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            8030..8512
                     /locus_tag="VXM85_001677"
     CDS             8030..8512
                     /locus_tag="VXM85_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1346225.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            8512..9132
                     /locus_tag="VXM85_001678"
     CDS             8512..9132
                     /locus_tag="VXM85_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1346226.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPADRILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(9385..9480)
                     /locus_tag="VXM85_001679"
                     /pseudo
     CDS             complement(9385..9480)
                     /locus_tag="VXM85_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2955 a         2077 c         1688 g         2988 t
ORIGIN      
        1 ataagttata gtgtagcatt ccaacttcaa agcactataa agtaaattga aacaagaaca
       61 atacaaacaa ttctcgtaaa cggattgcaa ccacaaaaaa gcaagcattc acaagaatac
      121 ttacctatca tgggaggaac aaccgttcct cttttttatt actaaaattc aaagaattcc
      181 aatgcttttt tcaagagcaa atccgtatat tctggatctt cttgggctac ttctatttcc
      241 cgctgaactt tttccaaatc atctgtaatc actccatcta ctcctaagtg aagagatttg
      301 ctgatagctt ctgaatcatt gacagtccag acataaagtt tctgatccgt tgtccatagt
      361 ttgcttacaa aatattcatc caaggttgag tactccatag tatatcctgt cgctcttgtt
      421 ttaggaaaga cagaattgta gggcatgatg aaataaactg gcagttcggc atcatactgt
      481 cttacttttt cgacaacatg gtagtctaaa gactggattt gatgtccata aatcttgagc
      541 tttgcagcat aacgggctaa aaagcggttc atcatgtctg gactatcttt tttactggtt
      601 ttaatttcaa ttagtaattt ttgaccaagt tcgttggctc gactgagata atcttcaaag
      661 cttgaaattt tagtctggta gccattttca aaaatatcaa tccctttaag ctcctccaag
      721 tttaagtctt gaggactttt attgatacct gctagatttt tcaagttagc atcatgcatc
      781 atgacaaact gcccatcttt tgtttcctgc acgtccgtct ccaccaagtc tggtttgagt
      841 tgtgctgtag tttccaagga ctctactgta ttttgaatcc catttgcatt ggaaacccct
      901 cggtgagaaa taagttgagg tagatgaacc atgggagcct ccagataaat ataaccttct
      961 aaggcaaaga aaagactggc acaagtcatg acaccccatc gcacgatgtg atctttttct
     1021 ctcctaggaa gcatatccag ctcctttcct gtcaaaaatg aaacaaattt aaccaaaaaa
     1081 taagtcagag ccatataata gagattttta atcacgacaa aattcaaaat accaagaatc
     1141 agagactctc tctgagtgat atcatctacc aaagtttgag ccaataataa agggatcaaa
     1201 ggaagataaa ataataaatg tgctttgagc aagatgtaaa ataaattcca agcataaaaa
     1261 gtaactctct tcttggtttt ctccaagcta aacatcactg cttctcgaac agtcagctga
     1321 tcatatacaa tcttcggaag ggcaaacatc aatctgacag agacatagag aaagataaga
     1381 gatagaagta ggatgctcag ccaccacatc caatatctat cttctaaata agcttggata
     1441 aactctggaa tgacgatttt attaagataa taaatcttca gcattttccg tataaaagga
     1501 aacagcatag ctatatagaa aaagataaac aaggctttag cgcaagttag ctttttcata
     1561 aatccaaaac tttcatggaa aaccttgcgg atatactcaa ttagccttcg cttttcatta
     1621 tagaggagat gacgagcacc aataaagagg agtcctattt gaaaataagc aaccagaagg
     1681 ttaattacaa tcaaggctaa aaaagctaga ctaatcaatg gagaatgagt aaggatggct
     1741 aagacattgt tataggaaat aaaaagataa cctgtctgat ctaataagaa gctagccaac
     1801 catgaattga atggtaccca caaatactcc actatcataa aaatcaagaa aaatagaaag
     1861 aggattttat caagattgag gtaaatctgt ttaagaccca attttttagg tttttcaggt
     1921 ttcataggca ctcctagtca aataattgag acaagtccaa gccaccaaaa ggattgtttg
     1981 ataagctact ttctgtctct aacaattccc tagcttgatc cgactctaag aaggattcgt
     2041 aaacacgcgc cgtcatccga gcatcctcta aactattatg agactgacct tgaaatccaa
     2101 gaaatgaggc aacagtttgc aatttgagat tggcaatacc atgtaaatct gaactccgac
     2161 gttcaaaagc ttcatcatac aaatccacct tgtactgttg gctatagtct aaaccatgct
     2221 ctgctaaaat aggtaaatca cttttagcag cattgtagcc tatcatcagc aaagaaccca
     2281 caaactcttg gaattcttct aagactttct caactggagg agcatctttt aaggtctcag
     2341 ctgtaatccc ggtcaaacca ttgataaagc tttttagagg gacgctggta tgaacatagg
     2401 aatcataggc atcgatttcc cgaccatctc taaaacgcac tgccgatact tgaatcaagt
     2461 gtgtttgtcc ttcgtgctga ttaaattcta agtcaaaagc aatgtaatct tctaatcgtt
     2521 ccattctgta cctctcctct actgtctctg ttctaatcaa ctatactatt cacaaacaaa
     2581 agaagccatc aggttagcat ttaacctaaa cagctccttg attatcctcc aaataaacga
     2641 gcaaaaaagc cttttttagt ggattggact tcttcttttg cttggtccag ctctagctta
     2701 agattttctt gatccttcat ggcttgaagg gtcaattgtt gttgctgatc taattgtttg
     2761 tctttctcag caatctgacg gtctttgata cgcatctgct cgtctttttc tgataactga
     2821 cgatccttgg cttttaattg ttcataaaga cgaagaattt ctgcattctt ctcatccact
     2881 agaatctcca tcagttcacg ttgtttgaca tcttcactga caggttcatc ttcaaaaatc
     2941 gtttttttat agatttcttc tagcttaatc aagccacttc tggtaacaac tgttacccct
     3001 ttgtcatttt tatctgtgtc ttcttctggt aattctttga cacggttatt gattgcttgg
     3061 cgagataatc ctaagacctc tgcaatctca ctgacggtca tttcaatact cataatatcc
     3121 tctgaaacgt tttctagctt ttctttactt aaatcttatc ataagctaga aaaactgtca
     3181 aatttccgct taatctaaag ccttcaaaaa ggccaacaag acttgggcag agcgacgtcc
     3241 agcttcgata ataaactcat caaaaaagat gtttgcttca tggttggcat tgtcactcat
     3301 agctctgatg actaagactg ggagattgag ggcatgcgct gcttgagcaa tagctgcccc
     3361 ctccatctcc acggctaaaa cttctgggaa atgggactta atcgcttcta tcttgtcatt
     3421 tcctgcaaca aaactatctc ctgtagcaat caaaccaaga tgccagtttt ggtccaattg
     3481 agataaactc tcttggattt gagcaacaaa ggttttgtct gattcgaaat aaagcggttg
     3541 ttgcgccatt tgtccataag catagccaaa agctgtgaca tccacgtcat gataggctaa
     3601 tttgtcagca atcacgacat ccccaacagc gataccttct gctactgccc cagctgaacc
     3661 cgtattaata agggcatcca cctggaaatg atcagccaaa atcgccacac tcatagcaga
     3721 catgacctta ccaattccac tttctacaag aacgacttca tgagaagcaa tggttcctgt
     3781 atgataggta ttccccaaaa caacttgctc ctgggcattg tctaaatgct ggaccagatg
     3841 agccagttct tctggcatag cagcaataat tcctattttc atttcaattc cttttctatt
     3901 acaaaagttt cattgctaaa acaagcaaaa tcaagagaaa gatgaccgcg aataagattt
     3961 tattcaactt agaattgaag acatttctct tggtattttc aatgcgacgg cttttatgaa
     4021 tagatggttc gacctgaatc ttcaaggttt cctggctaaa accatgatcc ttaggattgg
     4081 cataaccaaa acgggaagaa gaggttggta aaatcttagt ttcctcatta tcatccagca
     4141 aaggaggacc tgaaattttt tcgcctctat tagctctttc aatcatttca tccgttaata
     4201 aagatttacc catactgacc tcctctattt tttctgcaac tcccaatact gaacagccat
     4261 aattgtcttg gcatcacaga tatgacctga ttggattaat tctttcgctt cttctaagct
     4321 cacttcaagg acttccaagg tttcatcctc atcctgcgga cgcggatttt ccacttttgt
     4381 caaatcgctt gctaaatata gttttaactt ctcattacaa aagccaatag ctgaataaaa
     4441 atcgtacaag agttctaatt tccctgtata ggctgtttct tcctctaatt cacgaagggc
     4501 agctgccaca ggggctgtgt tttctcctac ctccaatttt ccggctggaa tttcgtaaga
     4561 gacagcctcg atagctttgc ggtactgctt gaccaagata agtttttgtt catccgttac
     4621 tgctaaaaca cagacagccc cattgtggaa aatcaaatcc cgttgggcag ttcccttgcc
     4681 ttctggtaat tcaacctgat cttggaccag tttaaatatt ggtccttgat agatttcttt
     4741 tcggctaagc gttttttctt caaactccat gataggctcc tactggttcc taggatgatg
     4801 aggaagacgt gttgcatatt cgtctttatt gatctgacga ccgcgaccaa tagcaatagc
     4861 atctgctggc acgtctttag taatagttga accagcacca acgagggaat tgtcaccaag
     4921 ttctactggt gcaataatgg ttgaatttga accaacaaag acattgtctc caatgactgt
     4981 cttgtatttg tttttgccgt catagttgac tgtaatagtt ccagcaccga aattaacgtt
     5041 gcttcccact tcacagtttc cgatataagt caaatgacca gccttggtat tctcaccgat
     5101 tgaagatcct ttcacctcaa caaagttacc aatatgaact tgggcaccca gacttgaatt
     5161 tggacgaatg tgagcataag gaccgactgt cacaccgtct gcaacactac tttcctcaat
     5221 catagaattg gtaatgaccg ctcctgctcc gatagtgctg tccactacat aagtaccgtt
     5281 tgtcaaaaca gtctcagcac caattttcgt ttgccctttc aaggtaacat tggcttcgat
     5341 ttgaacttcc ggagcaatct caacatcaat atcgatataa gttgcttctg gattgacaaa
     5401 gctaacacca ttgaccatgt gtttatgatt gatgcgacaa cgcataactg actcagctgt
     5461 cgcaagcgcc acacggtcat ttaccccaag actttcatca aaatctttca aagtataagc
     5521 gccaactttt tcaccagttt cacggaaaat accaatgacg tctgtaatat agtattcgcc
     5581 ttgagcgtta ttggtattga tatttttcaa agcctcaaac aaacgctcgt tgtcaaagac
     5641 gtatgttcca gtgttgattt ccttgatttg cttttcaaaa tctgtagcat ccttctgctc
     5701 aacaatacga agaacctcag cattgtcatt acgaacaatt cgtccatagc caaaaggatt
     5761 atccgtttca gcagtcaaga tagtggccac atttttatga ttgatatgga aatcaatcaa
     5821 gtttttcaag ctttcaccag tgattaaagg agtatctcct gcaatgacca aggtgtgtcc
     5881 tgacaaacct tctaagatag gctctgtcat cataactgca tgaccagttc ccaactgttc
     5941 agattgagtc acaaattctg tctgtccagc caagacctcc tcaaccaatt ctgccttgtg
     6001 tcctacaact gttactgtct tttcaggttg gatagctccc acactacgga aaacatgttc
     6061 caacatagaa atacccgcaa ccttgtgcaa aacttttggc aaatcagatt tcatgcgagt
     6121 ccctttaccc gctgctaaaa taatggcaaa atttgacata atcttctctt ttctgtagaa
     6181 attccctttt attataccat atttcaaatc attccgcact cttgaatgaa aaaatgctct
     6241 aaagtctctt ccttaaccca tttttttgat ttttagggta gaattataag atatgagaaa
     6301 acaaattcat ccacttattt tatttagttt tttggggatt atgatgttca ttttaatcat
     6361 caatcgccca cttaatgaca accaatcgtt taaaacaaaa tccaatatag cacagattga
     6421 agcacaggct ttgaaacact tagacaaacc gattattgac ctctctggct ggcagcgtcc
     6481 tgaggaaatc aactacgatg ccctctcaca aaatatttca ggtgctattg ttcgtgttca
     6541 cagtggtgct caaacgacaa aagaaaatga tgcttcattt atcaatggaa tagacaaggc
     6601 ctataagagt catatcactg aacttcaaaa gcggaatgtc ccagttgctg tctacgctta
     6661 tgtagctgga aaaagtgtcc aagaaatgga aaaggctgcc gaagttttct acaatgctgc
     6721 ctctccttac agccctagtt actattggct tgacgtggaa gacaaaacca tgtctaatat
     6781 gaacgaaggt gtcgaaaact ttcgagcaaa actagcatca ctaggtgcta aaaacatcgg
     6841 catctacgtt ggtgtctact tcatggaaga acacagtatt gatacaggca agtttacctc
     6901 tatttggatt ccgtcctacg gctcagactc aggatttttg gagagcagtc ctaaaactga
     6961 tttagattat gatattcacc aatacacatc taaaggaaaa attgccggct ttgaccacga
     7021 tttagatatc aacgttatct ctcccttaaa aaataaagaa gaaacctttc gaaaactttt
     7081 tttaaaacct taaaagcatt tgtttatcat ttgctttttc tttttgtgtc tgattgtgat
     7141 acaatatagt aaggattttt tgaaatagaa atagttggag gaaatatatg ctctcatggt
     7201 tagcacgcgt tattaaagga attgtgattg ctcttggatt tatcctaccg ggaatttccg
     7261 gaggggttct agcagcaatc ttaggaatct atgaacgaat gattggcttt ctggctcatc
     7321 cctttaaaga ccttaaagaa aatgttttgt actttattcc agttgccatc ggtatgcttc
     7381 tgggaatcgg cttattttcc tacccgattg aatacctgct tgaaaattat caggtctttg
     7441 tattatggag ctttgcggga gctattatcg gtacagttcc tagcctcctc aaagaatcaa
     7501 ctcgagaatc tgaccgagac aagattgatt tagcttggtt atggacaacc tttatcattt
     7561 ctggattagg actctattcc ttaaattttg tcgttggaac cttaagcgcc agctttctta
     7621 acttcgtcct agcaggcgca ctattggccc ttggcgtctt ggttcctggc ctcagcccat
     7681 cgaatttact tttgattttg ggactctatg ctcctatgtt gactggtttt aaaacttttg
     7741 atctcttggg aaccttcttt ccgattggaa ttggtgcagg tgcaactctc atcgtttttt
     7801 caaaattgat ggattatgcc ttaaacaact accactcacg cgtctatcat ttcatcatcg
     7861 gtatcgtcct atcaagtacc cttttgatct taattccaaa tacaggaaac gctgaaagta
     7921 tccaatacac aggactttca cttgtcggtt atgtcatcat cgccttcttc tttgcgctgg
     7981 gaatctggct tggtatttgg atgagtcaat tggaggataa atataaataa tggcaaaaaa
     8041 agttaaaatc aaaaaaacat tggtggaaca aatcctatct aaagcagcta tccctcatca
     8101 ggggattcaa atcaatgccc tagaaggaga gcttcctcaa ggttatgaac gagatcagat
     8161 tttcaaaacc ttggcgcttt tgggagacaa gaccggaccg attatcggaa ttgtccctat
     8221 cactcagcac ttgtctgaaa agaaactagc taaaatttct ggcaataaaa aagtgagcat
     8281 gattccacaa aaggacttag aaaaaacaac tggttacatt catggagcca ataatcctgt
     8341 cggaattcgt cagaaacaca attatcccat ttttatcgat aaaattgctc tagatttgga
     8401 tcgaatgatt gtctctgcag gcgaagtcgg acacagcatt atcgtcgcgc cacaagactt
     8461 ggctagcttt gtaaaggcag actttgtaga tattttggag gacatcaagt aatgaaactc
     8521 tactttgtcc gccacggtcg taccctctgg aaccaagaag gtcgttttca aggtgctagc
     8581 ggtgattctc ctcttcttcc tgaatccatt gaaaccctaa aacgacttgg ccagtatctc
     8641 aaggaaattc cttttgatca gatttattca agtgatttac ctcgagcggt caaatctgct
     8701 gagattatcc aaagtcaact ccataccccc tgttctttag aaatcgttcc taatctccgt
     8761 gaatggcaac ttgggaagtt agaaggtttg aaaattgcaa ccttggaagc tatttacccg
     8821 caacaaatcc aggcttttcg ttcaaatctt gctcaatttg acactcgaat gttcggagcc
     8881 gaatccctct atagtacaac tcagcggacc atccaattta tcaaatcatt aaaagatagt
     8941 ccagctgatc gtattctaat tgtcggacac ggcgccaatc ttactgccag tcttcgtact
     9001 ctcctaggct ataaagaacc acttcttcgt aaagatggcg gtctagcaaa tgccagcctg
     9061 accattatag aaacccatga ttttgaaaca ttcactctca atacttggaa tgatacttct
     9121 tatcaatcat aattatagcc tagccttctg aactagaacc ataggagata tagtcagttg
     9181 aaacaaaaat aagacaaaag aaccttgtaa aaaggtattg caacttgcca ataccttttt
     9241 gatatgaaca cgtgtcttct caacaggatt gtagagcaaa atgaaataag aactgaataa
     9301 tatgatttga gaatttaaat caatttataa gaatgtttta gaagcagtta tgtactattt
     9361 tagattcact tttctatact aagatgagta gggaggaaaa ggtaaaagtt tatgcccaaa
     9421 ttcttcacat aagaactcta gcttacccat tctatggaat cttgcattat ccattaaaac
     9481 ttccagactc atacccttct aaaatctatt caaaccacgt cagcttcaca ttatatatgt
     9541 tggtgacctc acaagtttta tctacaacct caaagctgtt ctttgagcag cctacagcta
     9601 gcttcctagt ttaatctttg cttttcactg agtatcaagt taaaaaaatg aggctggtca
     9661 aaaagtcttt aaaataaaaa acgcatatta tcaggtgtta aatgaact
//
LOCUS       DAVLRT010000070         9179 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLRT010000070 DAVLRT010000000
VERSION     DAVLRT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.070"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VXM85_001680"
     CDS             complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VXM85_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1346227.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2238..3107)
                     /locus_tag="VXM85_001681"
     CDS             complement(2238..3107)
                     /locus_tag="VXM85_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1346228.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3331..3951)
                     /locus_tag="VXM85_001682"
     CDS             complement(3331..3951)
                     /locus_tag="VXM85_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1346229.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKAPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEPTPEPGTPKTETPVNLDPEV
                     PTYETGKREGLSNTGTEANATLASAGIMTLLASLVLGFFKKKEDEK"
     gene            complement(3909..5015)
                     /locus_tag="VXM85_001683"
     CDS             complement(3909..5015)
                     /locus_tag="VXM85_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1346230.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVDSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5126..5380)
                     /locus_tag="VXM85_001684"
     CDS             complement(5126..5380)
                     /locus_tag="VXM85_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346231.1"
                     /translation="MLNVDQDFMSISKSNKSDSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(5738..6271)
                     /locus_tag="VXM85_001685"
     CDS             complement(5738..6271)
                     /locus_tag="VXM85_001685"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1346232.1"
                     /translation="MTIFKEQESEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(6258..6584)
                     /locus_tag="VXM85_001686"
     CDS             complement(6258..6584)
                     /locus_tag="VXM85_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1346233.1"
                     /translation="MAYNTDFKQGALDSIKGGHRHVEAAKVFWCWRQNSLHVGKERRE
                     QRNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIAAHFDCAVPSVWAALKQIKVTL
                     KKDDDL"
     gene            6818..8536
                     /locus_tag="VXM85_001687"
     CDS             6818..8536
                     /locus_tag="VXM85_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1346234.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKEIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8647..8994
                     /locus_tag="VXM85_001688"
     CDS             8647..8994
                     /locus_tag="VXM85_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1346235.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
BASE COUNT         2630 a         1886 c         1732 g         2931 t
ORIGIN      
        1 tatagtttgc tctttgattt tcattgagta ttagttcaat atctaaaaaa agaaccttgc
       61 caagcaagat tcttttagtg tctgacttaa ataattgttc ttctgggaac taaatcgaat
      121 taagcttcga tttctgtaac catacctgaa ccaacagtac gtccaccctc acggatagag
      181 aatgtagtac cttgttctac ggcgattggg tgaatcaact caacgtcgat tgtcacgtta
      241 tcaccaggca ttaccatttc agtacctgct ggaagttcga ttgaacctgt aacgtcagta
      301 gtacggaagt agaattgtgg acggtagttg ttgaagaatg gagtgtgacg tccaccttct
      361 tctttagtaa ggatgtagac ttcacctttg aatttagtgt gtgggttgat tgaacctggt
      421 ttagcgataa cttgtccacg ttcgatttca tcacgttgaa caccacgaag aaggacacct
      481 acgttatctc cagcaagacc ttcgtcaagt tgtttacgga acatttcaac accagtaaca
      541 actgcttttt gagtttcttc tttgatacca acgatttcga tttcgtcgtt gactttaacg
      601 ataccacggt cgatacgtcc tgaagcaact gtaccacgtc cagtgattga gaatacgtcc
      661 tcgactggaa gaagcaatgg tttgtcagtg tcacgttctg gttctgggat atactcatca
      721 actgtgttca tcaattccat aacgatgtct tcgtatttag agtcaccttc aagagcttta
      781 agtgctgaac cttggataac tggaagatcg tcacctggga agtcgtattc tgacaatagg
      841 tcacggattt ccatttcaac caattcaagc aattcttcgt cgtcaaccaa gtcaactttg
      901 ttcatgaaga cgataaggtg tttaacacca acctgacgtg aaagaaggat gtgctcacga
      961 gtttgtggca ttggtccgtc agttgaagct actacaagga tagctccgtc catttgagca
     1021 gcaccagtga tcatgttttt aacgtagtcc gcgtgtcctg gagcgtcgat gtgagcgtag
     1081 tgacgttttt cagtttcgta ctcaacgtgc gcagtgttga tagtgatacc gcgttcgcgt
     1141 tcttctggag cagcatcgat agacgcatag tctttaggtt ggttaactga tgaaggcaag
     1201 cgacgtgcca aaacagttgt gatagctgca gttagggtag ttttaccgtg gtcaacgtgt
     1261 ccgatagtac caatgttaac gtgtggttta ctacgatcgt atttttcttt tgccatttga
     1321 gtaaaagcct ccaataaaat atattttata gatagacagt aggcaataca gtctaacttt
     1381 ccttactatt ttatcaaatt tcagctaaat tgcaagtgtt ttacaacgtt tttgtgaatt
     1441 attcttggta aaaatttaac taattaatag aaagtactaa atagacttat ccttcaagga
     1501 gttttcagca ttatccatcc actcacttgc tgaaaatgta aacagtctct ggtttatata
     1561 gtaaaatgaa acaagaatag ggcaaatcga tcaggacagt caaatcgatt tctaacaatg
     1621 ttttagaagt agatgtgtac tattctagtt tcaatctact atacaacctc aaaactgtgt
     1681 tttgaacaac ctgcggctag cttcttagtt tgccctttga ttttcattga gtataaaatt
     1741 gcagataaaa cgataacaat cataggactc agcagagtca atgtaggtcg aagaatagcc
     1801 cccctaatcg cttattttat agcaaaatga aatagaaact gaacattttg ttgaggaagg
     1861 taaatcaatt cctcacaatg tttgagaaag aaggggggac tattccaact tcaacccact
     1921 ataaagcaga cttacaagat agggaaccca tctccgataa tattccgata atatatagta
     1981 gaatgaaaac tgcacaattt gattggagaa ttcaaaccaa tttctaacaa tgttttagaa
     2041 acaagggtct attatttcaa ctgcaaccta ctatagccca ttgaagctgg aatagcccac
     2101 tgagacttct aaaccactgc tggagaggag tttgtttaga gtttctttca tttccctaaa
     2161 aaatcaaaaa gagagaacca gtctgatcct ctcttactta tagatggtgc tcactcaaat
     2221 gttaaaggag ctatttctta gagataaagg aatttgaata cagctactgc cgcaattgct
     2281 gctgcgatag gtgctactac tggtacccaa gaataccacc attttgaatc gcctttatgc
     2341 tcaccaagaa ctgattttgg aaggaaagca tgaaggagac gtggtcccaa gtcacgggct
     2401 gggttcaagg caggtcctgt aggtcctcca agtgatgtta ccaaagccat aacgaggaat
     2461 ccaagtgcca agtgagccac agaaagtcct gaagcagtgt gtggagccac ctgtgcttta
     2521 atagccaagt cagaaaaatc aactgtttgt cctgcttcta ttgccttttg tttcatgaat
     2581 tgaagcactt cagcaccaaa gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca
     2641 aatgaaccaa caaactcatt aatcaaacca ttgacagttg ctgcatagcg actttctttt
     2701 gtaccatggt caatacttga aatagttgag aaagttccca agatgttatt tgggttttca
     2761 gttttcaagt agaatggacg gtatgttgcc acaactaagg cttggccaaa gatagccccc
     2821 aagacttgcg cgataatgta aggtaccact tgtgcccaag ggaaaagacc gctaactgca
     2881 agccctagag tgaaagcagg gttgatgtga ttcccagata cgttaccaaa catcaaggct
     2941 gggatcataa cccccatacc ataaccaaca gcgatgacga tccagccact ttggtgacct
     3001 ttcgtacctt taagttcaac gttggcaact gcaccattcc caagaatgat caaaatggca
     3061 gttcccaaaa attcagtggc atacttcagt gcccatgtga aatccatttg ctagattctc
     3121 cttaaaattt tttagatatt ccttatttta tcaattttta ccctatttag caactgattt
     3181 ctggaaatat ttttaaaaaa ataggattca tagtttccca gttcaacgga cttgtaagcg
     3241 tttttattct tcacgaaaga cctcgttctc aaaaccagga aaaaagccca catctgtgag
     3301 cttttcctgg ttcctagatt ctaaaatcta ttatttttca tcttcttttt tcttgaagaa
     3361 tcctaatact agactagcta acaaggtcat gataccagca ctagccaagg tagcattagc
     3421 ttctgtacct gtgtttgaca atccctctct cttacctgtc tcataagtcg gaacttctgg
     3481 gtctagattc actggagttt cagtttttgg agtacctggt tctggagttg gctctggtat
     3541 tggagtctct tgatttggta gttctgggtt tggtgtcgga acacttggat gctctggatt
     3601 tggttgtggg attggttttt ctggagttgg tttatctggt gttgataaac ggtcatacct
     3661 taccgttatt tctttatcac tagagtctga cgtaacttct tgtgattcaa ctgttggaat
     3721 atctggagct ttgtacttgt caatcttacc agatacaacc tcgtcccagt ttcctgttgt
     3781 ccattcaccg taggttacaa ctcccgtgac cttgttctca gtttttgtac ggcttaaggt
     3841 tacaggttga acaacatctt cttttacatt ttggttcgta actttatcaa cgtaatgaat
     3901 gatacgcgtt atagtcttcg tctcagtaga ggttgctgtt ttgggaacca ctgtttcctc
     3961 aacattctca cggtagtaat agtcaactgt tgcaccgtct tctggtacgc atttgcagga
     4021 gttgctacca aggtgtatgt tgttttcctt gtgataactc ggtcttcttt gtcctcagtt
     4081 gttgttttcc cttcaatagt ttttgattct gtggtatact cagaacctat cgctaaatca
     4141 gcttttataa cagactctgc caacttctct tggctacctt ctttatagta attggctgtt
     4201 actgtagcag tggttggcgc ttcgctttct ctataaacta aggtcactgt tctaccttcg
     4261 cttacaatat tcccagttaa acttgcagaa tttgtatctg cttctttaaa agtataatat
     4321 tttccgtcag tagtagtcat gctactgagt tttttatctg tgacattgta gctggtacca
     4381 atcagttgtt ttttattggt aatgtaggtt ccgtcacttt ctttttctcc aattccagta
     4441 tcatttttca atgatagcaa acgcccttgt tcatcaacat agcgaacttt cacattttct
     4501 gagattagtt ctgccaattc tgaggttttt ttctttttct tgatttcttc ggttattttc
     4561 cctttctctt cttcgggaat atttagtttt ggaatgattt tttcaacaac ggttcgtgat
     4621 ggttccacag tatcttggat gactgaaaag tcagctagaa ttgggagatt atactgaaca
     4681 cggtgacttt gagtgtttac tcctactctt tcattattct ctgaaaatac ttgtacggta
     4741 taagaaacaa catcttttcc taatagaaca tccccagtag agaaatagcc gccttttcct
     4801 agtttgctat ctccagagtc cacttctttc ctaatcttat cagatagttt tttaccagtc
     4861 agtacattcg ttcgcacaat ccctttgtct acccctacaa agtgggagaa ctttttgaac
     4921 tcttcagaac cagatctagc ccaaccatta ttaagggcat ttgcttttgt atttgtattc
     4981 tctctcaaag gtttggcgat tagaattata ttcatcggca cttagagttg ctgctatatc
     5041 tgactcttga ataccaactt ccttactacc atttctagcg gcagtatatg tgtatttatc
     5101 tgtttctact tctagctgat tatccttaac atgataggta attttctcct ggtataagag
     5161 tcttcagtag tttatatgct ctgtcatcat ctttagcttt agaagcagct gagtttagcc
     5221 aagttggggt attaggagta tctttatcag cctgtttcaa gacattattc aagtcatttg
     5281 ctaacctatt ggttgtcctc actgtgaaag ttttcttcca gtctgaatca cttttattag
     5341 acttagatat gctcatgaaa tcctggtcaa cattcaacac aatatcgcct tgttttttag
     5401 catcttccgt tgcttctgct tcaaattgat catggagttt ttgttgctct ggtgtatagt
     5461 ttttttattt acacgattta atgtactatt ttttatttta gtattaagaa ctccattaat
     5521 acgattttta aattctgctt caaaaaatcc cttaatttgt tggtgcgcaa gttcaacatt
     5581 caaacctttc ttatacaagt cttcattctg atacaagtct tcattctgaa cgatttcggc
     5641 tacacggtga atggtttgat cgtataccgt cttctcttaa ccagcagtat ccttagtttt
     5701 aatgactact cgataggttc ctttagaacc gttatagtca tctagtttta aaaaagcatt
     5761 ccaaacattc aaaaatagtt ggaacttccc ttaataaatc cgtcactttc tttttcaaga
     5821 tagcccaagc ttgctcaata ggattcaaat ccggtgaata aggtggtaga ggtaagaaaa
     5881 agtgtttatc ttggatgcag agttcatcca aaatgttctt gggatgaaaa ctagcattgc
     5941 ccatgacaat aacatgaggt gtcttcaaag caggtaggag ttgcgttttg aaccactcca
     6001 caaagaaatc gcttgtcatg cttttcttgt aaatcatggg agctataaac tctccgtcta
     6061 cttgtcctgc aacaattgaa gttcgctcaa aacgacgtcc gctaatcttt tcatagactt
     6121 tctcccctct aggagcccct gcataaggac gatagaggta gcggtcgatt cctgtttcat
     6181 caatatatac gattggtaaa tcttttaggt tatccaaaat atcaagaaac tcagcgactt
     6241 tctctgactc ttgttcctta aagatcgtca tcttttttta aagtgacctt aatctgcttt
     6301 aaagctgccc atactgaagg aacagcacaa tcaaaatgtg ccgcaatttc ccgtaaaaaa
     6361 gcatctggat gagcctctac aaaggctttc aattcttcta aagggatctt acgctttttg
     6421 acgactcgct ttttccgctc taagttcctt tgttcacgtc tttcttttcc cacgtgaaga
     6481 gagttctgac gccaacacca aaaaactttg gccgcctcga catgtctgtg ccctcctttg
     6541 atggaatcta atgctccttg tttaaaatca gtattatatg ccatagcttt attagaccat
     6601 gcttatttta aactaagcaa ctatatcaat gttttgttat gagaagactt acttttttta
     6661 ctgaaattga atttttccta gcctcaacat catataaccc caaaattcaa tctgacctgg
     6721 tttgaagaga tgttcgaagc agataaggtt ttactaaaac taaaaaaaat gatataatca
     6781 aactgattaa atgttttaaa aataaaggag aatttgaatg tcttaccaag aaaattacca
     6841 gaaatgggtt gattttgtgg agcttcctga ctaccttcgt caagatttgg aaaatatgga
     6901 cgaaaaaact aaggaagatg ccttctatac aaatcttgaa tttggtactg caggtatgcg
     6961 tggcttggtt ggtgctggta caaaccgtat caacatctac gttgttcgcc aagctactga
     7021 aggattggct cgtttgattg agtcaaaagg tggaaacgag aaagaacgcg gtgtagcaat
     7081 tgcctacgat agccgtcact tctcacctga gtttgccttt gaatctgcgg cagttcttgc
     7141 taaacacggc atcaaatctt acgtatttga aagccttcgt ccgactccag aactatcatt
     7201 tgcagttcgt catctcaact gtttcgcagg tatcatggtc acagccagcc acaaccctgc
     7261 tccatttaac ggttacaagg tttacggtga agacggtgga caaatgcctc cacacgatgc
     7321 ggacgctttg actacttata tccgtgcaat cgaaaaccca tttgcagttg aagttgctga
     7381 tgtggaaact gaaaaagctt ctggcttgat tgaagttatc ggcgaagctg ttgacgtaga
     7441 ataccttaaa gaggttaagg acgtaaacat caacccagcc ttgattgaag aatttggtaa
     7501 agacatgaag attgtctaca caccacttca tggtactggt gaaatgttgg ctcgtcgtgc
     7561 tcttgcccaa gcaggatttg actctgttca agttgttgaa gcgcaagcaa ctgctgaccc
     7621 agacttctca actgtaacat ctccaaaccc agaaagccaa gcagcctttg cccttgctga
     7681 agaacttggt cgtcaagttg gtgcagatgt tcttgtcgca actgacccag acgctgaccg
     7741 tgttggtgtt gaagttcttc aaaaagatgg tagctacctc aacctttcag gtaaccaaat
     7801 cggtgctatc atggctaaat acatcttgga agctcacaaa aacgctggaa ctcttcctga
     7861 aaatgccgct ctctgcaaat ctatcgtttc aactgacttg gtaacgaaga ttgctgaaag
     7921 ctacggcgca actatgttca acgtcttgac aggtttcaaa tttatcgctg aaaaaatcca
     7981 agaattcgaa gaaaaacaca accacactta catgatggga tttgaagaaa gcttcggtta
     8041 cttgattaaa ccatttgtac gtgataaaga tgccatccaa gccgttcttg tcgttgctga
     8101 acttgctgcc tactaccgtt ctcgtggttt gacacttgct gacggtatcg aagaaatcta
     8161 taaagaatat ggctactacg cagaaaaaac aatctctgtt actctttcag gtgtcgatgg
     8221 tgctgaacaa atcaaagaga ttatggctaa attccgcaac aatgctccaa aagaatggaa
     8281 cgcaacagct atcactgtcg tagaagactt caaggcacaa actgctactg ttgctgacgg
     8341 tactgttaca aacttgacaa ctcctccaag tgatgtgttg aaatacacac ttgctgacgg
     8401 ttcatggatt gccgttcgcc cttcaggtac agaaccaaaa atcaagttct acattgcagt
     8461 tgtaggggaa accaacgaag aatcacaagc taagattgct aacatcgaag cagaaatcaa
     8521 tgcatttgta aaataaagac ctataaaaga tgagaccaac caacctcatc ttttttcgta
     8581 tcaaatctaa gcaattttag aaactttatg gcatactaga cttatcaatg aaagcgaggt
     8641 aatctcatgg aacaattttt agataacatc aaagaccttg aagtcactac agttgtgcgt
     8701 gcgcaagaag ctcttgataa aaaagaaact gcaaccttct ttatcggtcg caaaacttgc
     8761 ccttactgcc gtaaatttgc aggtacattg tcaggtgtcg tagctgaaac caaagctcac
     8821 atttacttca tcaatagtga agaaccaagc caactcaatg atttgcaagc attccgctca
     8881 cgttatggaa tcccaactgt accaggtttt attcatatta cagatggaca aatcaacgtc
     8941 cgttgcgact cttcaatgtc agcacaagaa atcaaagatt tcgcaggatt gtaaaaaatc
     9001 agctaacaag tcttaaaatc acaaaaacgc ataatgtaag gtgcaaaaac cttgatatta
     9061 tgcgttttat tgttcctaaa acagttacat cagtatttca ttacagaaca ttttaatttt
     9121 ggaagtcaaa tcaattttta acaatgtttt agaaatcaca gtgggctatt ccatattca
//
LOCUS       DAVLRT010000071         9172 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLRT010000071 DAVLRT010000000
VERSION     DAVLRT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.071"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..743)
                     /locus_tag="VXM85_001689"
     CDS             complement(<1..743)
                     /locus_tag="VXM85_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1346236.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGFLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMT"
     gene            complement(758..1606)
                     /locus_tag="VXM85_001690"
     CDS             complement(758..1606)
                     /locus_tag="VXM85_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1346237.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(1603..3078)
                     /locus_tag="VXM85_001691"
     CDS             complement(1603..3078)
                     /locus_tag="VXM85_001691"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1346238.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(3401..4756)
                     /locus_tag="VXM85_001692"
     CDS             complement(3401..4756)
                     /locus_tag="VXM85_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1346239.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMAYKTEL
                     S"
     gene            complement(4766..5083)
                     /locus_tag="VXM85_001693"
     CDS             complement(4766..5083)
                     /locus_tag="VXM85_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1346240.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(5100..5417)
                     /locus_tag="VXM85_001694"
     CDS             complement(5100..5417)
                     /locus_tag="VXM85_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1346241.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(5432..6328)
                     /locus_tag="VXM85_001695"
     CDS             complement(5432..6328)
                     /locus_tag="VXM85_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1346242.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(6331..7593)
                     /locus_tag="VXM85_001696"
     CDS             complement(6331..7593)
                     /locus_tag="VXM85_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1346243.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDILLYLPFVIAIEKRFKLLED"
     gene            7873..9051
                     /locus_tag="VXM85_001697"
     CDS             7873..9051
                     /locus_tag="VXM85_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1346244.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALFYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         3196 a         1951 c         1293 g         2732 t
ORIGIN      
        1 gtcattccca tgacaaagaa taacatccct ttaaaacagt atcttaccca attttcaggt
       61 aagacttttg atacaaccgt ccccaatacc cctccgcata tagaagcaag aaaaataact
      121 ggaacaggtg ctaaatgata ctgatttaaa cctgtaacca caacaattgt agccaaatga
      181 gacaactggg aaaagaaaat aattgcaaga gaatacatcg ttgcttcttt tatcgaaata
      241 gaaaaaaaca ccatcaataa agatacattg agcggaccac caccaatccc taaaaaacta
      301 gaaatcaagc ccaatagaaa accacaaatc atatagaatc ctatcttatc aaattgataa
      361 gtttttttaa aatctgttaa aacaaatgat acaagtagca tcacaaatag taaaatcatt
      421 tgaacaatgg atactagccg tactgataat tgagataaca aaacttgaaa aataagatga
      481 cctagaaacc ctcctaaaac agaagaaaag gataccgtaa aaatcaatct ccattttatc
      541 tctttacttt gtgaaaatct tttggttgtt gaaataatcg ccattataaa aacagagaaa
      601 ctagaataaa aagcaatatc actgaccgaa tggtatccaa aactatccat aagtggctta
      661 attataattc caccacccat gcctgatata gaacccaatc cactagctat tataatgatt
      721 agaaaataaa taatctcaac cactttttaa gtccatccta ttctttcaaa catctaaaac
      781 ctagatggct actagtggaa gtagaaatac atccatttct agcagctact cggtatcgat
      841 tacaataaga acaatgacat aaaaaggaac ctcctctgat agcatactcc tcatcttgta
      901 tcccatattt tggcaactca tagtcatctt cattaaaact agctaataag gtatatctcg
      961 gattgcgaca ccattcccaa acattcccaa tcatttgata tagaccaaaa tcattaggta
     1021 aaaattcata gacaggagcc gttcctataa atccatctaa ggcagtattt tcataaggaa
     1081 atcttccttg ccaagtattg gcatgataat atccaccttc taaaagagta tctccccaag
     1141 gatattcaga ttgtcttcct cctctagcag catattccca ctgtgcttca gtcggtaaag
     1201 acattccgct ccaattacag aaagccaatg catcttctaa agcaacatga actactggat
     1261 ggtcttctaa acccactaga ttactattct caccgtaggg atgtttccag caagcgttcg
     1321 gaacttgtaa ccaccacgga gccccagcag gatgaggata tccctccctt tctgcctctg
     1381 gaacgaacaa tataaaaaca aaagaccact cttgtcgctc agctagtgtt ttatagcctg
     1441 tttctttcac aaattgagca aattcttgat tcgtaactgg agtatccgca atggaaaaat
     1501 cctttacact aacagcgact tgaggacctt caaaatcatc taaaaagccc tcttcagaat
     1561 ttgttcccat cataaatgtc ccacccttga tttttttcat tttcatgatt gactatcccc
     1621 cgttttcgtt agagttggcg ttattttaga aagttcaaca ggaactaact cttcatccac
     1681 aacaaaacct tcttctctat atctaagaaa gtctactaat tttttcttca tctgacgaac
     1741 aataggttgg tatttttcac ttggatacaa atcattcatt tcatgcggat cttttttcat
     1801 atcaaataac tgataatgat ttaaaacagg aaaccagata aatttccact ggtctgtcaa
     1861 gatatattga ctagaatctt ttcctaaagc atgttcacca tgaaattctg ttcgccaccc
     1921 ctcgtactgt ccaaataata aattcttaac actccgtcca tctagttcat ctgtagtagt
     1981 tcctcctgcc aaatccacga gtgaaggaaa aatatcttga attttcacta attgcttgat
     2041 tgttccccta ttaccagcta tcaatccagc cggatcgtaa ataaacgaag gaatgtggat
     2101 acttccctga taaggatatc cctttctaaa cagataatgt tcaccaagct gatctccatg
     2161 atccgaaaca aaccagataa tcgtatcttt atcatgacga aattctttta gggcagtcaa
     2221 aaagcgacta atttgatggt ctatatgagt aatcaatcca aaataagcag caaccattct
     2281 tctttgatca tcttccttta atttccctct taaagcataa atagacggaa tttgcttttc
     2341 taatacttcc caattcccga tatgcaaatc gagaaattga ggcaagcgtt ccatatacat
     2401 atcaaaatag tattttgggg gatttaatgg agcatgtggt ttttcaaaag acattttgag
     2461 aaaaaaagga accgttggat ctcttctttg taaaaatgaa atggactcac tcacaaccca
     2521 gtttgttgga tgtaacttct catctttatc ccaaggtcta gcttcccaag aattacaatc
     2581 cattccgtca tcaatcaaat caacatcata accaacttta ccctttaaaa aggctagata
     2641 gtcactagca taatcaaatt gacttccata agccttatca tactttctat caacatgtag
     2701 atacccatcg tgaagtaaga catgatcaaa cccaagtctc tgtctggaag gaaaaacatg
     2761 catcttacca atacactctg tttgataacc catatcttta aagacttttg gaagagtatt
     2821 agtaaaattc caaggcacct catcttgata accaactcgt ccacttttat cttggtctaa
     2881 gccagtcaat aaagctgccc tagcaggaac gcaagaagga actggagaat aggcattttc
     2941 aaaattataa cctacactag ccatcatatc taatgttgga gtgctaacta gcttatcctt
     3001 actattcaag gataaggcgt ctgctctcat ttgatctaca acaatcaaaa taatatttgg
     3061 ctgttttgtc tgaaccataa aatctccttt ctaatatggc aaaagaggca caagaagata
     3121 tctaccttta ctgcacccct ttctatatca atctctctat ataaaacaat aacattcttg
     3181 ttatgtttta tagaaaaatg aactaaaata tgactaaatc gattaggaaa ttcaaatcat
     3241 tttctagtac tgttttagaa gttacagtgt actattccaa cttcaataaa ctataaacct
     3301 ttgtctaata acaattttag tggagataag aaatcctaca cctaactcat cttacacgta
     3361 atctatttct attttatcac aaaaaacgca agtaagacca ttaactcaat tcagttttat
     3421 atgccatttt cacaaatggg aaataagtca agacactaat aatcaaacaa acaactgata
     3481 agatgatggc acgccaatca aatgctgtag agaagaaacc atataaaatt ggaggcatta
     3541 cccaagtaac gttttgtgta acaggtgaaa caagacccca gcttgttgcc cagtaagcta
     3601 ccgttgccat gaaaaccgga ctaagtacaa atggtataaa tagcaaagga ttcaagacaa
     3661 ctggtaaacc ataattgacc ggctcaccaa tattaaacag aactggtgct agaccaagtt
     3721 tagcaacttt tcgataatga ctgtttcttg aaaaaattaa aatagcaagt actaatccta
     3781 atcctccaaa ccagacaaac gccccaaaag acccacttgt ccatatataa ggaatcggtt
     3841 cacctttttg gaaagcatcc agattcgcta acatagcaac tccaaacagc ccttccatga
     3901 tgggagccaa tacatttcct ccatggagac caaaaaacca gaataactta ttcaaaaaga
     3961 tcatcagaat aactgcaaag aaactttgag acaaacctag taatggcgtt tgtaacacct
     4021 tgtaaaccca atcaatcaat aagtcattgc taagtaaatg gaaaacataa gtcaagatgg
     4081 ctactatata catcgccata aatcctggaa tgatagaagt gaacggctta gcaatcgcag
     4141 ggggaactga atctggtaac ttgattaccc agttcttttt cattacttta cagaaaataa
     4201 tagaggctaa aaatccaatc atcatggctg taaagtagcc tctggcatta atatggtttc
     4261 ctggaatcac attcccaata gttaccatca gatttttacc atcaaatgct agattatcaa
     4321 ttccttgtaa gatttgatct aatttcacat ctcctacatt tgccaaaggg aaactctttg
     4381 taactgtact tccaatcgaa atgacaaacg aagcaagtga taccaaacca gcagaaactg
     4441 tatcaacctt gtaaatctta gcgatattca ctcccaagca atagatgaac aacaaggaaa
     4501 caattggtat acttcccttg aataccaaat tattgatgtc aacaagccac tgaaaggttt
     4561 tcgtaatact ttctaggtga aattgttgtg gtaaatccac tagaaaagca tttaataaca
     4621 aagcaatgga acctgtcata ataacaggca tagtccccac aaatgaatca cgtaaagcaa
     4681 ctaaaaactt ctgattccca actttcattg caattggaga aagtttttct tgcattttat
     4741 ccaacattgt attcatacca aactcctaat ttttttcaaa tcgttcgtat acttctacca
     4801 cttctttcgc caagtcaaca aaagtcagac tcgtcattaa atgatcttgc gcgtgtacca
     4861 tcaagaggct aattgatgtt ttttccccct gtgcctctct tgtcaacatt tctgtttgag
     4921 ccttatgcgc ctgtaataag gcagcattcg catcagctaa tcttcgattg gctttagaga
     4981 aatccccttt ttttgctgcc tgaatagcct ccatagcatt acttttggct tccccaccat
     5041 acataattag ccccattaca gattctaaat tactttcatc cataacattc tcctcaatct
     5101 tagtctaata aactttcagc taaatctaaa actttagaac cattcatcat tccgtaatct
     5161 gtcatcggaa taaccgccac aggaatctgt ctatctttta gtttttcttg aaagtcccct
     5221 agtaaatagc gaacttgagg acctaaaagc aacacattta cttcttttgt tgctacaatt
     5281 tcttctgctt caggagctgg aactgcaaaa atttctgcat ccaacccctt atcttctgct
     5341 gccttttgca tctttgtcac tagcagactt gtactcatac ctgctgcaca tgctaacata
     5401 attgttactt tagccatagc ttactccttt atcacctatt tactgaccta gccaagcgag
     5461 atactgtatg tctcactcct ctaattgcct tgctcaatgc ataaatatta tcgactgttg
     5521 ccagtccccc atatcctgcg tcacctatat gttgaatatc aactccacaa attttatttc
     5581 taagtgcaat ttccttgata gtatctgtat cagatgtttc ttggctagta ccaatagcac
     5641 tcaatacaag accaccctta ctatgaacga gatcaacgac ttcacgcaac tcttggtcat
     5701 gaaaggctgg aacggttcca actgctggaa caagtatcac atccgcacct gcttccaaca
     5761 attgctctgc gacagaaagc tctgccacag gctcattcac tcctgccccg tgcatcttac
     5821 ctgcaataat caaaccagaa aagttttcct tagcagtttg aacagcctta atgatttctc
     5881 gattgctaac tcctgttcca ggatttccag tcaaacagac aaagtcaaaa cctaactcct
     5941 ctattcgatt caatgtttca acactagcaa cacgacctgc aacaatttcc tgtgtttcct
     6001 ctagcatctt tgcagatggg tcaatcggtt ccaaatttac accaattgga caagcaacaa
     6061 gctggtgtaa gcgatgaata acatcatcac ctgaactatc caaagcataa attttaggct
     6121 caaaaacatc aacacaattc aataaaatca tatcagctcc aaaagctcta gcaatttcag
     6181 aattagttat atccccaaca aatgtctcac gagtaactac attttcagat agaacaactc
     6241 taccttcact tgctaaaata ctttgtttta attcttcagc attcatctgt aatatctcag
     6301 atggatttgc actaattaaa cgtttcattc tcaatcctcc aataacttaa atcgtttttc
     6361 aatagctatt acaaatggta aataaagtaa gatatccaaa acaatcaata caatttgtag
     6421 taatgaacca gaaatacttc ctgtagcaag taaaccactt ataacaggtg gcatagtcca
     6481 acctggatct gtataagtta gcggaaccaa acctgatgcc attgctcccc acgctactag
     6541 taagttaaac attggagcta agataaatgg aactagtaac aaaatgttca aaacaatagg
     6601 aacgccaaac attgctggtt catttatatt aaataatcca ggtataacag ttataggtgc
     6661 caaagttttt agttgtttac tagctttctt cttatgtgct aaatatccta acgcaagaac
     6721 taaacctatg gttgcaccac cgccacctat gaatacaaaa ttatccataa atacgctagt
     6781 aaaaatattt ggaagagttt ctccagtttg ataagctact ttatttgcat ctagattagc
     6841 taaccataac ggtttcataa ctgcattaac aacatttcct ccgtgtaatc ctacaaacca
     6901 aaaactacta ttaaggaaaa ttaagataag aagaccaata acattatttc ctaaaagtcc
     6961 aagtggacct cccaaagcta cttgagcaat ttcatgaaga ttaggtaaac ttaatttatc
     7021 taaagttgca taaacaatca accaaccaac aatagtcact gcaccaggaa taatggcact
     7081 aaagctttta gatactgctg gtggaacacc atccggcatt tttatctgta tattatgatt
     7141 tataaaccat tggtagatat agccattaat caatcctaaa atcatagcaa caaataaacc
     7201 ttttgatgcc atgaaagcgg taggcattcc tgctccagcc tctcctctaa tgaaaggcgt
     7261 aactgtaata aaagatgata atgacacaat accagcagga ataccatcaa ctttgtattg
     7321 tctagccata aagtaagcga taccaaaact agctacaaga ccaagtaatc caaaactact
     7381 atcaacaccc ttccataaat agtcagcaac accaatatca cccagatatt tttcccatcc
     7441 tggaatagga aaacttgcaa taaccattag taatgaacct atcaacaata aaggtatagt
     7501 tagagtaata ccgtctcgaa tagaaaccaa cgctttatta ttacccaatc tagccgctac
     7561 tggaagtaac ttttcttgta ataatttttc cataaccttt tcctcttaaa tatattttat
     7621 tatcaaaagt tatcaattat ctttattttt tgacgtcaag ttgttatttg attatctttt
     7681 gttatttata ttttacaact ataagaaagc cttgtcaagg atttgtgtaa acatttgatt
     7741 attttttatt atcaatatat aataaaaaga gaccagtaag taatgaaaag cttaaaaaga
     7801 ttaacatcta catttatcca tagcaaaaaa tcataggatt ttaagaatct gacaactagg
     7861 aagaaaacta atatgacctt atctaagaaa caattacaac taagagctaa aatactggaa
     7921 acagtttaca cattaggtcc catttcacga attgaaatcg ctactaaaac aggaataaca
     7981 cctgcaacaa ctagcagtat cacaaatgat ctgattaaag aaaatattct cttagaactt
     8041 ggtgaagacg aacacgatac aagcgtaggg agaaaaaaaa tcctgctaga cattcaagct
     8101 aaacgatttt actacattgg ttgtgaacta tctgaaaaac atttcacttt tgctttagga
     8161 gataatctag gaaatatctt aaaggaagag aaagaaattg ttactaaaca gttaatccaa
     8221 gaaaagggaa atcaactcat taaccagaca ctaaagcaat tcttaaataa ctgctctgat
     8281 tatgaaatag aagcaattgg aatcgcgcta cctggtcgct atttagatga ttataaaata
     8341 acaacaaaca atcctttatg gcaacatatc gacttagaaa tgattcaatc ccactttgat
     8401 aagcctttgt ttttctctaa caatgttaac tgtatggcta taggtaaacg cctatttagt
     8461 cgccaacaga atgaccctaa ttttgcttat tttcattttg ctagaggtat gcactgttct
     8521 tatatctacg acggaaacat ttatgggaaa ggaaatctaa tgattggaga aatagggcat
     8581 acagttgttt catctgaggg agaagaatgt agctgcgggc gcaagggatg cttacaaact
     8641 tttgctggag aatcttggtt aatcaaaaaa tcaaaaattc tatatcatca atctccctat
     8701 tctctacttc ctagtttagt caaaaatgcc gatgatattg atatccaagt tattttaacc
     8761 gcttatcaat taggtgacac tggaataatc acacttatcc atcaagcctt attctatctt
     8821 agtcaaacca ttttaaatat cagtatgatg attgactctc aaaaaatcta cctacatagt
     8881 cctttactaa caaatcaaca catcatccaa aaactatata gtgaaatgaa ctacaaaccc
     8941 aaactgctct ataatcgcct acctgaagtc attattgaac cttacaatga ttttacagcc
     9001 gcacactcag ctattgcact ttgtttgtat catacaattc tacattctta attttctatt
     9061 acatttaata aataggctaa aaagagggag tatatcataa aaagcgaata aacctgtttt
     9121 ctgaaaatca gaattctgtt ttgttcgctt tctttatatt tggaattata ct
//
LOCUS       DAVLRT010000072         8996 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLRT010000072 DAVLRT010000000
VERSION     DAVLRT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.072"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(381..1229)
                     /gene="dprA"
                     /locus_tag="VXM85_001698"
     CDS             complement(381..1229)
                     /gene="dprA"
                     /locus_tag="VXM85_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1346245.1"
                     /translation="MKITNYEIYKLKKSSLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPDSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1325..2014)
                     /locus_tag="VXM85_001699"
     CDS             complement(1325..2014)
                     /locus_tag="VXM85_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1346246.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEIFKNEN"
     gene            complement(2026..2904)
                     /locus_tag="VXM85_001700"
     CDS             complement(2026..2904)
                     /locus_tag="VXM85_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1346247.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2894..3763)
                     /gene="licA"
                     /locus_tag="VXM85_001701"
     CDS             complement(2894..3763)
                     /gene="licA"
                     /locus_tag="VXM85_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1346248.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIEFAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3780..4802)
                     /locus_tag="VXM85_001702"
     CDS             complement(3780..4802)
                     /locus_tag="VXM85_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1346249.1"
                     /translation="MINQIYQLTKPKFINVKYQEEVIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4807..5514)
                     /locus_tag="VXM85_001703"
     CDS             complement(4807..5514)
                     /locus_tag="VXM85_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1346250.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLHKERIIITKGGADRNTSIEKIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5850..7337
                     /gene="tacF"
                     /locus_tag="VXM85_001704"
     CDS             5850..7337
                     /gene="tacF"
                     /locus_tag="VXM85_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1346251.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7347..8150
                     /locus_tag="VXM85_001705"
     CDS             7347..8150
                     /locus_tag="VXM85_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1346252.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8152..8961
                     /locus_tag="VXM85_001706"
     CDS             8152..8961
                     /locus_tag="VXM85_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1346253.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2822 a         1814 c         1513 g         2847 t
ORIGIN      
        1 tataaattaa tttgactttc ctgatagagt tgttcacatc ttatttcaat tcactatact
       61 ttcccttata ctcaatgaaa atcaaagcgc aaactaggaa gctagccaca ggttgctcaa
      121 agcactgctt tgaggttgta gataagactg acgaagtcag ctcaaaatac tgttttgagg
      181 ttgcagatgg aagctgacgt agtttgaaga gattttcgaa gagtattata tgagaaaaat
      241 cctctactct actaatccac ttagaaaaaa cgcatattat caagtgttaa aaacttgcta
      301 atatgcgttt tattatggaa atgttgggtt cattttctct taaaattgat tttttcttag
      361 aattctagct taggtcattt ttaaaattca aattccgcaa gaacatcttg cccactggtg
      421 accaattttg ctccttcttg aatcaaatga tggcaaccgt ctgatagtcc atctaaaatg
      481 ctatcaggaa tagcaaagac atcgcgtcct tcttccattg ctcgctcaca cgtaatgaga
      541 ctacctgaac gcatcttagc ctctgctaca atcacaccac gacaaagtcc agcaatgatg
      601 cgattacggg caggaaaatg aaatttcaga ggttgttcgc caggtccata ttcacttaga
      661 accagatggt cattgccgat gtagtcttgc aagcgtttat tggctttagg ataaaacaca
      721 tccagtcctg ttccaatcac tgcaatggtt tttccgccat tctgaagagc tgccatatga
      781 gctgctgtgt caatgccctt ggccagacca ctgacaataa ccagttcatt ttccaagcct
      841 tgaatgactt tttcaactga cttagctccc tgtttgctac aagcacgact gcccacgacc
      901 gctaccttcg ggaatttcag gaggtcaaga tttcccttgt aaaataaaag tacaggcgca
      961 tcatatattt cactcaaatc ccaaggataa cagtcatcta aaatagagaa agatggaaat
     1021 ttttgaaact ctttcgacaa atgcgcatcg tctatctgaa aataacgttc cataaaaacg
     1081 gctggattac ggcaacctga tatatctgca atatcaccca acaaaagctc ctgatcaaca
     1141 ttttcaccgt attctagaac tttcaaaatc tgttgattgg tcaaacttga tttttttaac
     1201 ttatagattt catagtttgt gattttcata aataactcca tttctttttc tactcatcta
     1261 tctattcgta aaagttcgta aaaaatcaaa aaacatccga ctattttgtc agatgttgga
     1321 atctttaatt ttcgttttta aaaatttctt ctaatttacg atagtcttgg acactatcga
     1381 tctcataaat gctattgcct tctaattctt caacatagac atctagctct ttgatattat
     1441 ccttaaccat attgtcccaa tagagatcaa caaattcgcc acttgcataa gccttatcga
     1501 taaagctgac aatcttttct gcagttggag catcccagaa ggatacacca ctaaggatgc
     1561 gacctgcctt gctatcaaca ataatgtctt gaaccttgta gtcatctcca tagaccaaga
     1621 accattcgtt ggtacaatct tcacgataaa cactaaaata agtcgaacga gtcaaatcat
     1681 tgcggaacat atttttaaag agataattgt cagcatcaat aacatagctg ttggccaatt
     1741 cttcttttac aagatagaga gagtaaaagt tattgtagtc agcgtattta tcattgaaaa
     1801 cgaggcgaac accgtatttt tcttttaagt aatcgaattg ttctttaaga taaccaacaa
     1861 tgatgatgat gtcattgatt cctttttctt tgagaaactc aatttggtac tcaattaagg
     1921 gtttttgatt aacctgaacc aaggctttag gggtattttc agtcatagga cgcaagcgag
     1981 ttcccaatcc cgctgctaag ataatggctt tcacacgaat ctcctttatt ctttaataat
     2041 aatataaact ccagcaatga cgacaagtga cgtcataatg gtcagcatat ctagcggtgc
     2101 acccaagaaa acaactgcaa acaagaccgt ccatactaca tagctcacgt tcaagcctgt
     2161 agccttggct ggttgcaagc gattgatagc gatataataa gccaagtagg aaatcatatc
     2221 aaaggctgca aaaacaatca tgagacctag caattgtcca ttggctactg cagtaaatga
     2281 ctgatgagag aagagcacaa tcacaagata ggacaagaac gaagttactt gacggattaa
     2341 gagggcttcg atttcactca attcactttc catggcaaaa gagctaagaa cactctcact
     2401 tccccatgca atagcacaaa ccaaagcaca aagaatccca atgtagaaag aattaacctg
     2461 ttcaacctta taggtctgag caataatccc tccaataatc aagacaatcc caaatacagt
     2521 atttttcgaa atcttgtgct tcaaaaagaa gaaagccaat agaactgaaa tcgcagggta
     2581 aatagccgat acagatgaag ctaaagaact tccgatatac ttaactgcat aaagattggc
     2641 ctgcataccg atagggcctg ctagcaaggc tccgatgata acactgacat tgcgaatatt
     2701 taagaaaatt gagaggcgaa ctttcccttc ttttaccaag agaaaagcta gtaagataaa
     2761 gatgctcaaa aaatcatgag ctgcagccac cacaaagggt gacaaatctg taaaaatcga
     2821 aaagatataa gcactaaccg ttagacccaa gccccagaaa atacctgaga gaaggccaaa
     2881 aggaactccg tttttacttt tcatctgaac ctccataaga agccaaacct ttaacagctc
     2941 tttggtaacg attcacacca tagtcaccaa aatcttcacc ttgctcttcc ttatagacag
     3001 tccatagact ccaaatagta tcttgtaaaa ttttataaat agcaatcttt tcatgagaaa
     3061 ccggtgtttg gtcactctca tagtgagata agaaagtttc ttcctcttgg gaagtgaatt
     3121 cagactctaa aaagagggca gccaaatccc acattggatc attcattgat gaatattccc
     3181 agtcaatcaa ataaagtcgt ccttgaggtg attcgataaa gttttcaggc accaaatcga
     3241 tatgacaaga ttttctgtca acacctaagt cagccagtct tttctctaag gagaagactg
     3301 catttctaac agattcatag ttggcataag gaatttgttc ttcaatcaag gattcgtatt
     3361 ttttgatttc ttcaaaagga gcaaattctc ctcttaattc cttagcagac gtatgaatag
     3421 tttgtaatat tggagcaatt ttgtcgaact tggtcttgat tgacgttgaa tcaagcgtaa
     3481 tcgcaaattc gatatactca tttactttga taccagcttc aatatcaaaa agataatttt
     3541 ttacatctaa gcctaaatcc tttagtagtt caagattgta cttttcatct tgtcgattga
     3601 taagcttttc tgtcccttta ccaaagaatt taacaatgta ttgcttattt gttgttttgg
     3661 ccaaatagtt ttgattggtc attccaccca gttgttcaac actgaggact tcctcttctt
     3721 gactaagtaa ggaagaaatt ttttctttaa tgattttctc cacaattaac ctccagtact
     3781 tatacttccc acttaaacac tgttttaaag gctgtgttta aatcggttgc aaagacacga
     3841 tgaatatctt taatttctct tacaggttct tctagataaa ggatattttt aagacgattg
     3901 gcaaatttct tgacttccat catttggata gcattttcaa aatcaatgcg accagaacga
     3961 gatgacccaa ccaaaatcaa gcccttttct aaggcatcgc gagtattgag attgacttta
     4021 tattcgctaa ctcccatcat gagaatcgtt ccctgaggac gaatgtagcg aatcaagtca
     4081 ttaatagctg gtccagtacc atcaccacca caacattcaa aagcatggtc aaaggccaaa
     4141 tcttcaggaa tattatccgt aatatagcat tctttggcaa atgagaagag ttccaacttt
     4201 tcccaatgac gaccaataac cacaatctct gcttctggca aagtatagtt gataatattg
     4261 gcaaccacaa aagctaaact tccatctcca ataacggcga tccgctcccg cttgctatga
     4321 gcaagagtca atagacgatt catagcgtgc atgcccacac tgacaaactc tgtaatggct
     4381 gcaaccgtat cttcaatagc atcataagcc accacacgat ctttagggag agaaacaaac
     4441 tctctcataa agccatcaaa tccactagac aagaaatggg tccctgtcat gtagttttca
     4501 tagaattctt catcactctg cataggagac tgattgggaa tcatgacaac tttttgacca
     4561 acctcgtagg ttccggtcgg gtcagaaatg acggttccac atgactcgtg aatcattgcc
     4621 attggaagct ttttattcaa aatcttggga tcacgttttc cctgatagta acgctgatcc
     4681 gcatgacaga cagccatgta gttgggacgg ataaggatat gattctcttg gtcaataacc
     4741 tcttcctgat atttgacatt gataaactta ggcttagtta gttgataaat ttgattaatc
     4801 attttactag tctttctcaa tcatactttt tgcaatcttc aaatctgtta cggttgtaat
     4861 cttcagattt gagtattcac ctttggccaa ggccacatct tttcctttga tcacaaagat
     4921 tttacatgca tctgtcaaga tttccttctc ttcatcagaa agagatccat aaaggtccat
     4981 gaagtccttg caacggaatg tttgaggtgt ttgtccttga taaaggtgag cacgatttgg
     5041 aatatctgta ataaattgac cattggtact ttcaacgata gtatcaaccg cttctaccac
     5101 tgtgtccact gcgtcatgat tttgggcaag ttggatattg tcctgaatca tgcgaagtgt
     5161 aataaatgga cgaacagaat cgtgggtaac aacgatatcc tctgaagtaa gcggacgata
     5221 agcatcaatg gcttcaatga ttttctcaat acttgtattg cggtcagcac caccctttgt
     5281 aatgatgata cgttccttgt gaagaggaag atatttatct acaagatctt ctgcatgaga
     5341 aacccagtct ccatgaaccc caactacaat tttttcaata cttggctcca agacaaattt
     5401 ttcaattgta tgaatcaaaa taggtcgatc acctagctct aaaaattgtt ttggcaagtt
     5461 actgattccc atgcgtgtgc cagttccacc ggcaagaatt cctgcataaa tcatctattt
     5521 tctcctttgt cttactaaaa aaacttattc tctctattat accacaatct agtcctatca
     5581 taaaagaaat actggtcctc cccttctaga ataggagaat tatgcagttc tattattcaa
     5641 agaaactagt tcatttctag taatgactag gaattctgta gcaattacta ataaatggct
     5701 gcgaaatttt agagtccttc agaataatat actttcctta aagaaaactt aaaatttaag
     5761 atttgaaact tcaaagtgat tacttgactt actgcccttc atttcttatt agctaacttt
     5821 atgatataat gaaaaacgag gtaaattgaa tgaaaagtat aaaattaaat gctctatctt
     5881 acatgggaat tcgtgtcttg aatattattt ttcccatcct aactggaacc tatgtcgcgc
     5941 gtgtcttgga ccgaactgac tatggttact tcaactcagt cgacactatt ttgtcatttt
     6001 tcttgccctt tgcaacttat ggtgtctata actacggttt aagggctatc agtaatgtca
     6061 aggataacaa aaaagatctt aacagaacct tttctagtct tttttatttg tgcatcgctt
     6121 gtacgatttt gaccactgct gtctatatcc tagcctatcc tctcttcttt actgataatc
     6181 caatcgtcaa aaaggtctac cttgttatgg ggattcaact cattgcccag attttttcaa
     6241 tcgaatgggt caatgaagct ctggaaaatt acagttttct cttttacaaa actgccttca
     6301 tccgtatcct gatgctggtc tctattttct tatttgttaa aaatgaacac gatattgttg
     6361 tctatacact tgtgatgagt ttatcgacgc tgattaacta cctgattagt tatttttgga
     6421 ttaaaagaga catcaaactt gttaaaattc acctaagtga ttttaaacca ctctttctcc
     6481 ctctgacagc catgttagtc tttgccaatg ccaatatgct cttcactttt ttagatcgcc
     6541 tcttcctcgt taaaacaggg attgatgtca acgttagtta ctataccata gctcagcgaa
     6601 ttgtgaccgt tatagctggg gttgtaacag gtgcaattgg agtgagtgtg cctcgtctca
     6661 gttactatct ggggaaagga gacaaagaag cctatgtttc tctggttaat agaggtagtc
     6721 gaatctttaa cttctttatc attccactga gttttggact catggtttta ggaccaaatg
     6781 ccatcctact ttacggtagt gaaaaatata tcggaggcgg catcttgacc tctctcttcg
     6841 cttttcgtac gattatcctg gccttagata ccattcttgg ttcccaaatt ctctttacca
     6901 atggctatga aaaacgtatc acagtctata cagtctttgc tgggctactc aatttgggct
     6961 tgaatagtct cctctttttc aaccatatcg tggctcctga atactactta ctgacaacta
     7021 tgttatcaga gacttctcta cttgttttct atatcatttt catccataga aaacaactca
     7081 tccacttggg acatatcttt agctatactg ttcgatactc tctcttttca ctttcctttg
     7141 tagcaattta tttcctgatt aatttcatgt atcctgtgga tatggtcatt aatttgccat
     7201 ttttgattaa tactggtttg attgtcttgc tatcagctat ctcttatatt agtctacttg
     7261 tcttcacaaa agatagcatt ttctatgaat ttttaaacca tgtcctagcc ttaaaaaata
     7321 aatttaaaaa atcttaggag tttaaaatga aacaactaac cgttgaagat gccaaacaaa
     7381 ttgaattaga aattttgaat tatattgata ctctctgtaa aaagcacaat atcaactata
     7441 ttattaacta cggtactctg attggggcgg ttcgacatga gggctttatc ccttgggacg
     7501 acgatattga tttatccatg cctagagaag actaccaacg atttattaac atttttcaaa
     7561 aggaaaaaag caagtataag ctcctatcct tagaaactga taagaactac tttaacaact
     7621 ttatcaagat aaccgacagt acaactaaaa ttattgatac tcgaaataca aaaacctatg
     7681 agtctggtat ctttatcgat attttcccta tagatcgctt tgatgatcct aaggtcattg
     7741 atacttgtta taaactggaa agcttcaaac tgctgtcttt cagtaaacat aaaaatattg
     7801 tctataagga tagcctttta aaagattgga tacgaacagc cttttggttg ctccttcgac
     7861 cggtttctcc tcgttatttt gcaaataaaa tcgagaaaga aattcaaaaa tatagtcgtg
     7921 aaaatgggca gtatatggct tttattcctt caaaatttaa ggaaaaggaa gtcttcccaa
     7981 gtggtacctt tgataaaaca atcgatttac cctttgagaa tttaagcctt cctgcacctg
     8041 aaaaatttga tactattttg acacaatttt atggagatta tatgacccta ccaccagaag
     8101 aaaaacgctt ctacagtcat gaatttcacg cttataaatt ggaggattag gatgcaatat
     8161 ttagaaaaaa aagaaattaa agaaattcaa ctagccctgc tggactatat tgatgagact
     8221 tgtaagaaac atgatattcc ttattttctc agttatggaa ccatgcttgg agccatccgc
     8281 cacaaaggta tgattccttg ggatgatgat attgatattt ccctttatcg tgaggattat
     8341 gagcgtttac tgaagattat tgaagaagaa aatcaccctc gctacaaggt tctttcctac
     8401 gatacatctt cttggtactt ccataatttc gcatcgattt tggacacttc tactgttata
     8461 gaagaccatg ttaagtacaa gcgtcatgat accagccttt tcatcgatgt cttcccaatt
     8521 gatcgattta cagacttgag cattgtcgac aagagctata agtatgtggc tcttcgtcaa
     8581 ctagcttata tcaaaaaatc acgagcagtt cacggtgata gcaaactaaa agattttctt
     8641 agattatgta gctggtacgc tctccgattt gtcaatcctc gctactttta caagaaaatt
     8701 gatcaactag tcaaaaatgc tgtaaccaac actcctcaat atgaaggagg agttgggatc
     8761 ggtaaggaag ggatgaaaga aatcttccca gttgatacct ttaaagaact gattttaact
     8821 gagtttgagg gccgtatgtt gcctgttccc aaaaaatatg accaattttt aacccagatg
     8881 tatggcgatt atatgacacc accatcaaaa gaaatgcaag agtggtatag tcatagcatt
     8941 aaagcttatc gcaaaaactg attgaggggt attatacaaa ctactaagat agaggt
//
LOCUS       DAVLRT010000073         8841 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLRT010000073 DAVLRT010000000
VERSION     DAVLRT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.073"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            19..855
                     /locus_tag="VXM85_001707"
     CDS             19..855
                     /locus_tag="VXM85_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1346254.1"
                     /translation="MKRKELRLSQQTLAEGICEQSQISKIERGHFIPSADLLFKLSQR
                     LEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERSTFLTLEDRT
                     YLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFYSLVGREQEY
                     EANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEALETIELCKQ
                     RQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEAKELCKIYNNPLMLMKIENYLKELD
                     TV"
     gene            1238..1858
                     /locus_tag="VXM85_001708"
     CDS             1238..1858
                     /locus_tag="VXM85_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346255.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1944..2387
                     /gene="tsaE"
                     /locus_tag="VXM85_001709"
     CDS             1944..2387
                     /gene="tsaE"
                     /locus_tag="VXM85_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1346256.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2377..2895
                     /locus_tag="VXM85_001710"
     CDS             2377..2895
                     /locus_tag="VXM85_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1346257.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQAGGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2903..3919
                     /locus_tag="VXM85_001711"
     CDS             2903..3919
                     /locus_tag="VXM85_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1346258.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4004..5260
                     /locus_tag="VXM85_001712"
     CDS             4004..5260
                     /locus_tag="VXM85_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1346259.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5315..6481
                     /gene="recA"
                     /locus_tag="VXM85_001713"
     CDS             5315..6481
                     /gene="recA"
                     /locus_tag="VXM85_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1346260.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            6788..8158
                     /locus_tag="VXM85_001714"
     CDS             6788..8158
                     /locus_tag="VXM85_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1346261.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     YIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8534..>8841
                     /gene="lytA"
                     /locus_tag="VXM85_001715"
     CDS             8534..>8841
                     /gene="lytA"
                     /locus_tag="VXM85_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1346262.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     R"
BASE COUNT         2679 a         1490 c         1969 g         2703 t
ORIGIN      
        1 ctcgcagaga aattcagatt gaaaagaaaa gagttgagac tctcccaaca aactcttgca
       61 gaaggaattt gtgaacaaag tcagattagt aaaattgaga gagggcattt cattccctcc
      121 gcagaccttt tgttcaaact ctctcaacga cttgaagtac cattagatta tttttttaat
      181 gaacaaattg aaattaaatc taacctctct aatttcaagc aattgtctgc tcgactatta
      241 gacgatagaa attatgacga tttggaatat atttatagaa tagagattga acgaagtact
      301 tttctaacac tagaagaccg aacttatctt gaatggatta aagctattat tgacttttat
      361 caatatgaca gtaagtgtga agcaatttct tcattggaaa atatattatt aaaagtctcc
      421 tcaaatactc tgatttattt aaaggcgttg aatactctat ctaatttcta ttccttagtg
      481 ggtcgtgaac aagaatatga ggcaaactac tctcatttaa tagagttgta tcagacaaaa
      541 aatttggacc atcaagagtt tttatttggc tacatcagag ttcgttacaa ctacgctcac
      601 tacctagtat caaaggaaaa atataatgaa gccatccaag aagctcttga gacgattgaa
      661 ctctgtaaac aaagacagac aagctaccaa ctggctcctc tacttattct tgtaggaaat
      721 gctggagcca aatttctaga cagagaacaa gtcaaaaatt attatataga agcaaaagag
      781 ttatgtaaga tttataacaa tcctttaatg ctgatgaaga tagaaaatta tttgaaggaa
      841 ttagatactg tttagttaat cttgacatta tagtttttct gaaacgttaa ataacctgta
      901 aggctgatag tgaaatgaat actatcagcc ttatgattat gtgactatta gttcgtctcg
      961 ctccgttagg tgcgagacaa aaaaaacacc cgctagacgg attttcatga tttcaaattt
     1021 atattcttcg aaaatctctt caaatcacgt cagctctatc tgcaatctca aaacagtgtt
     1081 ttgagcaacc tgtggctagc tacctagttt gatctttgat tttcattgag tattagtctc
     1141 tagtgtttgt ttagcgtctt aaagagacat ttgagaagat tagaaacagt tctctctttt
     1201 ctcctagtat aatgattggt aggaagggag agagaagatg aaatcaatga gaatcttatt
     1261 tttgttagct ttaattcaaa tcagtttgag tagctgtttc ctatggaagg aatgcatctt
     1321 gtcctttaaa caaagtacag cttttttcat cggaagcatg gttttcgttt caggaatctg
     1381 tgctggagta aattatcttt atacccgtaa gcaagaagtc catagtgtcc tagccagtaa
     1441 gaagtcggtg aagctttttt acagtatgtt actcttaatt aatttgttag gagctgttct
     1501 tgttttgtca gataacttgt tcatcaaaaa tacgctgcag caagaattag ttgacttttt
     1561 attgccatcc ttctttttcc tatttgggct agatttgctg atttttttac ccttgaaaaa
     1621 atacgtgcgc gattttcttg ctatgctaga cagaaaaaag acagtgttgg tgactatttt
     1681 agcaacactt cttttcttaa gaaatccaat gaccattgtc tcacttctga tttatattgg
     1741 actgggcttg ttttttgcag cctatcttgt cccaaattcg gttaagaagg aagtttcctt
     1801 ttatggtcat attttccgag atcttgtatt ggtcattgtt acgctcattt tcttttagag
     1861 aaagcctgtt ttttatggat gttatgatga taagtttagt aggaaaatag acacaaaaga
     1921 aaagtttttg gtataataag agtatgtaca caaaaaatga agaagagttg caagccttag
     1981 gggagcgttt gggccatcta ttagcaaaga atgatgtttt aatcttaact ggagaactgg
     2041 gtgcaggtaa aacgaccttt actaaaggac ttgcaaaagg attacagatt tctcaaatga
     2101 ttaaaagtcc cacctatact atcgtgagag agtatgaagg tcgacttcca ctttatcacc
     2161 tagatgttta tcgtattgaa ggagatgctg attctatcga cttggatgag tttatctttg
     2221 gtggcggcgt gactgttatt gagtggggaa atctcttagg agatgccttg ccagatgctt
     2281 atttagaatt ggaaattcta aaagaagcag atggacgccg tttaaatttt caggcaaagg
     2341 gtttgcgtgc tgagaaattg ttagaggagc ttcaatatgg agtatgaatt gctcattagg
     2401 gaagcagagc ccaaagatgc agctgaatta gtggcctttt taaatcgtgt gagtttggag
     2461 acagacttta ccagcctaga cggagatggt attctcttga ctagtgagga gatggaaata
     2521 ttcctcaaca agcaagctag ttcggacaat cagataacct tacttgcatt cttaaatggc
     2581 aaaattgctg gtattgtaaa tattacagct gatcagcgta agagagtccg tcatattgga
     2641 gatctcttca ttgtgattgg aaaaagatat tggaataatg gcttgggaag tttgttgcta
     2701 gaagaagcga tagagtgggc acaagcaggt ggcattctgc gtcgtctcca actgactgtc
     2761 caaactcgta atcaagcagc agtccatctt tatcaaaagc atggctttgt cattgaaggt
     2821 agccaagagc gtggggcata tatagaagaa gggaaattta tcgatgttta cttgatgggt
     2881 aaactgatag gttagtagaa atatggttaa aaaaattatt ggaatggtgc tagctttact
     2941 ttctgtaact gtagtaggag taggtgtttt tgcttatact atttatcaac aagggacaga
     3001 aaccttagct aaaacctata aaaaaatcgg cgaagaaacc aaggttattg aagcgactga
     3061 acctctaacc attctgttaa tgggagtgga caccggaaat gctgaacgaa ctgaaacttg
     3121 ggtcggtaga agtgatagca tgatcttgat gacagtgaat cctaaaacga aaaaaacaac
     3181 aatgatgagt ttagagcggg atattctgac gcgcattgaa tcagggaatg gtcaggctca
     3241 tgaagcgaaa ctgaactcag catatgcaga tggtggagca gagcttgcta tagaaaccat
     3301 tcaaaaaatg atgaatatcc atattgatcg ctatgtaatg gtcaatatga gagggttgca
     3361 aaaactagtg gatgcagtag gaggtattac agtcaataat atcctaggtt tcccaatttc
     3421 tatcagtgac caagaagaat ttaatactat ttctatcggt gttggggagc aacatattgg
     3481 gggagaagaa gccctagtct atgcacgaat gcgttaccaa gatcctgagg gggattatgg
     3541 tcgtcaaaaa cgtcaacgtg aagttattca aaaagtcatg gaaaaagctc tcagtttaaa
     3601 tagcgttggt cattatcaag agattctaaa agctttgagt gacaatatgc agaccaatat
     3661 tgatttgtct gcaaaaagta tccctaactt gctaggctat aaagattcat ttaaaaccat
     3721 tgaaactcag cagttgcagg gtgaaggaga gatacttcaa ggtgtttctt accagattgt
     3781 ttcgagagca catatgttgg aaatgcaaaa tatactccga cgttctttgg gacaagaaga
     3841 agttactcag cttgaaacca atgcggtttt atttgaagat ttatttggca gagcacctgt
     3901 tggtgatgaa gataattaac ttttgataca aataaaaaaa tcaatcgtag gaaaattcct
     3961 gcgatttttt caaaaaaata cgaatagata ggtaggagga aacatgaaag cagaaatcat
     4021 tgctgttgga acagagattt tgacaggaca gattgtcaac accaatgccc agtttttgtc
     4081 ggaaaaacta gctgagattg gggtagatgt atattttcag acggctgtag gagacaatga
     4141 agttcgtctc ttgtctttgc ttgagattgc cagtcaacgt agcagtctgg tgattttgac
     4201 aggcggtttg gggccaactg aggacgacct aaccaaacaa accctagcta aatttttagg
     4261 gaaagcatta gtctttgatc ctcaggctca ggagaagttg gatatctttt ttgccctgcg
     4321 accagactat gcccgaacac cgaataacga aagacaagct caaattgtag aaggagcgat
     4381 tccactgcca aacgaaacag gactggctgt tggaggaaaa ttagaagtag acggagtgac
     4441 ctatgtcgtc cttccaggtc cgccaagtga attgaaaccc atggtcttaa accaacttct
     4501 acccaagttg atgacaggga gcaagctgta ttcccgagtt cttcgtttct ttgggattgg
     4561 cgagagccag ttggttacga ttttggctga tttaattgat aatcagatcg atcctacctt
     4621 ggccccttat gccaagacag gagaagtcac tctacgtctg tcaacaaagg ctagcagtca
     4681 agaagaggcg aatcaagcgc tggatatctt ggaaaatcaa atcttggact gccagacttt
     4741 cgaaggaatt tctttacgag acttttgcta tggttatggg gaagaaacta gtttagccag
     4801 cattgtggta gaagaactga aaaggcaagg gaaaaccatc gcggctgcag agagtttgac
     4861 ggcaggtctt ttccaagcta ccgtggcgaa tttttctgga gtttcaagta tatttaaggg
     4921 tggttttgtg acctatagct tggaggaaaa atcaaggatg ttggatattc ctgccaagaa
     4981 tttggaagaa catggtgtgg tgtctgaatt tacagctcag aagatggctg agcaggcacg
     5041 aagcaagacc cagtctgatt ttggaattag tttgaccgga gtggcaggac cagatagcct
     5101 agaaggacac ccagttggga cagtcttcat aggcttggcg caagagcaag gaactgaggt
     5161 tatcaaggtt aatattggag gcagaagccg agcagatgta cgtcatattg cggttatgca
     5221 tgcctttaac ctagttcgca aggctttatt aagtgactaa cttttgatat aatagtagat
     5281 aggtctgagg atcattagaa tgtaggagaa tagaatggcg aaaaaaccaa aaaaattaga
     5341 agaaatttca aaaaaatttg gggcagaacg tgaaaaggcc ttgaatgacg ctcttaaatt
     5401 gattgagaaa gactttggta aaggatcaat catgcgtttg ggtgaacgtg cggagcaaaa
     5461 ggtgcaagtg atgagctcag gttctttagc tcttgacatt gcccttggct caggtggtta
     5521 tcctaaggga cgtatcatcg aaatctatgg cccagagtca tctggtaaga caacggttgc
     5581 ccttcatgca gttgcacaag cgcaaaaaga aggtgggatt gctgccttta tcgatgcgga
     5641 acatgccctt gatccagctt atgctgcggc ccttggtgtc aatattgacg aattgctctt
     5701 gtctcaacca gactcaggag agcaaggtct tgagattgcg ggaaaattga ttgactcagg
     5761 tgcagttgat cttgtcgtag tcgactcagt tgctgccctt gttcctcgtg cggaaattga
     5821 tggagatatc ggagatagcc atgttggttt gcaggctcgt atgatgagcc aggccatgcg
     5881 taaacttggc gcctctatca ataaaaccaa aacaattgcc atttttatca accaattgcg
     5941 tgaaaaagtt ggagtgatgt ttggaaatcc agaaacaaca ccgggcggac gtgctttgaa
     6001 attctatgct tcagtccgct tggatgttcg tggtaataca caaattaagg gaactggtga
     6061 ccaaaaagaa accaatgtcg gtaaagaaac taagattaag gttgtaaaaa ataaggtagc
     6121 tccaccgttt aaggaagccg tagttgaaat tatgtacgga gaaggaattt ctaagactgg
     6181 tgagcttttg aagattgcaa gcgatttgga tattatcaaa aaagcagggg cttggtattc
     6241 ttacaaagat gaaaaaattg ggcaaggttc tgagaatgct aagaaatact tggcagagca
     6301 cccagaaatc tttgatgaaa ttgataagca agtccgttct aaatttggct tgattgatgg
     6361 agaagaagtt tcagaacaag atactgaaaa caaaaaagat gagccaaaga aagaagaagc
     6421 agtgaatgaa gaagttccgc ttgacttagg cgatgaactt gaaatcgaaa ttgaagaata
     6481 agctgttaaa gcagtggaga aatccgctac tttttcgatt tttgattcaa gtttttagat
     6541 tatatatagt agattgaaat aagatatgaa caactttatt aggaaagtca aattaatttc
     6601 tagaaatgtt ttagcagcta cagcgtacta ttccaaactc aaccaactat aatagatcga
     6661 aactagaata gtacacatct acttctaaaa cattgttaaa aatcgatttg actgtcctta
     6721 tttcattccg ctatatataa gtttgctgtt tcttgtcgct cctctggaaa gctgatataa
     6781 tagctttatg aataaaaaac gaacagtgga cctgatacat ggtccgattc ttccctcgct
     6841 cttaagcttc acctttccaa tcttgttatc aaatattttt caacagctct ataacactgc
     6901 tgatgtcttg attgttggac gatttcttgg tcaagaatcc ttggctgcag taggagcgac
     6961 gacagcgatt tttgatctga ttgtaggttt tacacttggt gttggcaatg gcatggggat
     7021 tgtcattgct cgttattatg gggctcggaa tttcactaaa atcaaggaag cagtagcagc
     7081 cacctggatt ttaggtgctc ttttgagcat tctagttatg ttgctgggct ttcttggctt
     7141 gtatcctctc ttgcaatact tagatactcc tgcagaaatt cttcctcaat cttatcaata
     7201 tatttctatg attgtgacct gtgtaggtgt cagctttgct tataatcttt ttgcaggctt
     7261 gttgcggtat attggtgaca gtctagcagc cctgggattt ctgattttct ctgccttggt
     7321 taatgtggtt ctggatctct attttattac gcaattgcat ctgggagttc aatccgcagg
     7381 acttgctacc attatttcgc aaggtttatc agcggttctc tgcttttatt atattcgtaa
     7441 aagtgtgcca gaactcttgc cacagtttaa acatttcaaa tgggacaaaa gcttgtacgc
     7501 ggatctcttg gagcaaggtt tggctatggg cttgatgagt tcaattgtat ctatcggcag
     7561 tgtgatttta cagttttctg ttaatacatt tggtgcagtg attattagtg cccagacggc
     7621 agctcgacgc attatgacct ttgcccttct tcctatgacc gctatttctg catcaatgac
     7681 gacctttgct tctcagaatc taggagctaa gcgacctgac cgcattgttc aaggtcttcg
     7741 aatcggcagt cgtttaagta tatcctgggc agtttttgtt tgtattttcc tcttttttgc
     7801 cagtccagcc ttggtttcct tcttggctag ttcgacagat ggttacttga tagaaaatgg
     7861 aagtctctat ctgcaaatca gttcagcctt ttatcccatt ttgagcctct tgttgattta
     7921 tcgcaattgc ttgcagggct tggggcagaa gatccttcct ctagtttcta gctttattga
     7981 actaatcgga aaaatcgttt ttgtggtttt gattattcct tgggcaggat ataagggtgt
     8041 tatcctttgt gaacctctta tctgggttgc catgacagtt caactgtact tctcactatt
     8101 ccgtcatccc ttgataaaag aaggaaaggc aattttggca accaaagtgc aatcctagtt
     8161 ggatttactg aataaaatcc atttcctcta gtgaacatcg aaaaaacttg tgttctcttc
     8221 ttcagtttgg tgttgaaaat agtttaacag acttttgact tcttttatat gatataataa
     8281 agtatagtat ttatgaaaag gacatataga gactgtaaaa atatactttt gaaaagcttt
     8341 ttagtctggg gtgttattgt agatagaatg cagaccttgt cagtcctatt tacagtgtca
     8401 aaatagtgcg ttttgaggtt ctatctacaa gcctaatcgt gactaagatt gtcttctttg
     8461 taaggtagaa ataaaggagt ttctggttct ggattgtaaa aaatgagttg ttttaattga
     8521 taaggagtag aatatggaaa ttaatgtgag taaattaaga acagatttgc ctcaagtcgg
     8581 cgtgcaacca tataggcaag tacacgcaca ctcaactggg aatccgcatt caaccgtaca
     8641 gaatgaagcg gattatcact ggcggaaaga cccagaatta ggttttttct cgcacattgt
     8701 tgggaacggt tgcatcatgc aggtaggacc tgttgataat ggtgcctggg acgttggggg
     8761 cggttggaat gctgagacct atgcagcggt tgaactgatt gaaagccatt caactaaaga
     8821 agagttcatg acggactacc g
//
LOCUS       DAVLRT010000074         8801 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLRT010000074 DAVLRT010000000
VERSION     DAVLRT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.074"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            44..199
                     /locus_tag="VXM85_001716"
                     /pseudo
     CDS             44..199
                     /locus_tag="VXM85_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            399..3674
                     /gene="rexB"
                     /locus_tag="VXM85_001717"
     CDS             399..3674
                     /gene="rexB"
                     /locus_tag="VXM85_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1346263.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTDYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYCFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3671..7321
                     /gene="addA"
                     /locus_tag="VXM85_001718"
     CDS             3671..7321
                     /gene="addA"
                     /locus_tag="VXM85_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1346264.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAANELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWELAKTFQTFMSHFVEVYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYIFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLTSLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7491..8669
                     /locus_tag="VXM85_001719"
     CDS             7491..8669
                     /locus_tag="VXM85_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346265.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERDIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2721 a         1848 c         1872 g         2360 t
ORIGIN      
        1 ctatttttgg ttcattttac tatagttcaa tcattctatc aagaatattg aatatataaa
       61 ggcacgtaat attaaaactt cttctgatat cgaattagag tccgatgttg atggcgataa
      121 atcagacaat ctacccgtcg acattaagac actgggacaa cacattgaag tgtattccgt
      181 acctaagaaa caatcataaa actcaaagcc ttgttaccga ggctttttta ttatatgatt
      241 ttcagtggcc agacttattc tatctaatac aagtaaagct aagtatttaa gatttagcag
      301 atgatgaaga aaagaaagga tggcctaaat attttaacag atttacgagg ctaatcatgg
      361 tataatatag ggtgatgaaa aattccaacg aggctgagat gaaattactt tatactgata
      421 ttcgaacttc tttgacagaa attctaacaa gagaggcaga agagctagtt gcagctggca
      481 agcgggtctt ctacattgcc cccaactctc tttcttttga aaaggaacgc gccgtgctgg
      541 aatgcttgtc ccagcaggct tctttttcga ttaccgtcac gcgctttgct caaatggctc
      601 gttatctggt cttgaatgat ttaccagtta aaactactct tgatgatatc ggtcttgggt
      661 tggcctttta caaatgcctt gccgaactcg atcccaagga cttgcgtgtt tatggagcta
      721 ttaagcagga tcctcaattg atccagcagt taattgagct ttaccatgag atgaccaaat
      781 ctcagatgaa ttttttggac ttggagaatt taacagatga ggataagagg gcggatttac
      841 tcttgatttt tgagaaagta acagactatc ttaatcaagg tcagttagcc cagggaagtc
      901 agttgtccca tttgattgag gctattgaga atgacaaggt aagtagtgat tttaatcaaa
      961 tcgccttggt cattgacggc tttactcgtt tttctgctga ggaagagcgg gttgtggact
     1021 tacttcacgg caaaggtgtt gagattgtta tcggggctta tgctagtaag aaagcctata
     1081 ccagtccttt tagcgagggc aatctctacc aagccagcgt aaaatttctc catcatctgg
     1141 cttctaaata ccaaacgccc gctcaggact gttctcaaac tcatgagaag atggatagtt
     1201 ttgacaaggc ctctcgtttg ttggagtctt cttatgactt ttcagaactc gctttggatg
     1261 tcgatgagaa agaccgtgaa aatttacaaa tctggtcttg tttgacgcaa aaggaggagt
     1321 tggagctagt agcccgtagc attcgtcaga aattacatga gaactcagac ctgagctaca
     1381 agcattttcg tattctcttg ggggatgtag cttcttacca gttatctctc aaaaccattt
     1441 ttgaccagta tcagattcct ttttatcttg gtagaagcga agccatggct catcatccct
     1501 tgactcagtt tgtcgagtct attttagctt taaaacgtta ctgttttcgt caggaggatt
     1561 tgattaatct tcttagaact gacttgtata ctgacctcag tcagtctgat attgatgctt
     1621 ttgagcaata tatccgctat cttggtatca atggcttgcc agcctttcag caaatcttca
     1681 ccaaatccca ccatggaaaa tttaatcttg agcgtttgaa tgtcctccgc ctgagaattt
     1741 tagcacctct tgaaaccctc tttgccagcc gaaaacaaaa ggctgaaaac ctcctacaaa
     1801 aatggagtgt ctttctaaaa gaaggagctg tgaccaagca gttacaagat ttgacaacca
     1861 ctttggaagc tgtagaacag gaaagacaag ccgaagtttg gaaggctttc tgccatgttt
     1921 tagaacaatt tgcgactgtt tttgctggtt cacaggttag tctggaagac ttcctagcct
     1981 tgctccattc tggaatgagt ttgtcccaat accgtaccat tccagcaaca gtggacactg
     2041 ttctggtgca gagttacgat ttgattgcac cattgactgc tgactttgtc tatgctattg
     2101 gactaactca ggacaattta ccaaaaattt ctcaaaacac cagtcttctg acagatgaag
     2161 aaaggcaaaa tctaaaccaa gcgaccgaag aaggcgttca attactgatt gccagcagtg
     2221 aaaatctcaa gaaaaatcgc tacactatgc tttccttagt caattctgct cgtaagcagt
     2281 tgttcttgtc ggctccaagc ctttttaacg aaagtgaaag taaggaatct gcctatcttc
     2341 aagagttgat ccattttgga tttaggcgga gagagaagag gatgaatcac aaaggactgt
     2401 ctaaggagga tatggggtcc tatcacagtc ttttgtctag tctggttgcc tatcaccagc
     2461 agggtgagat gagcgatact gagcaagatt tgacttttgt caaggttctg tcgcgtgtca
     2521 taggtaaaaa actagatcag caaggtctgg aaaatccagc catcccaatc agtccaagca
     2581 gcaagacctt agccaaggac accttgcaag ctctctatcc tgccaaacag gagttttacc
     2641 tgtctacgtc gggtttgaca gagttttatc gcaatgaata cagttatttc ctacgctacg
     2701 ttttaggctt gcaggaggaa ttacgtttgc gtcctgatgc ccgtagtcac gggaatttct
     2761 tgcatcgtat ctttgaacgc gccttgcagt tgcctaatga agattccttt gaccaacgtc
     2821 tagaacaagc tattcaagaa accagtcaag aacgcgaatt tgaagctatt tatcaagaaa
     2881 gtttggaagc ccagtttacc aaggaagttt tgcttgatgt tgcacggaca actggacata
     2941 ttctccgaca caatccagcc atcgaaacaa tcaaagaaga agcaaatttt ggtggaaaag
     3001 accaagcctt tattcaatta gacaatggac gcagtgtctt tgtacgaggc aaggtggacc
     3061 ggattgaccg tttgaaagct aatggagcga taggagtagt agactacaaa tccagtctga
     3121 ctcagttcca gtttcctcat ttctttaatg ggctcaattc tcagttacca acctatcttg
     3181 ctgccctaaa aagagaaggg gagcagaact ttttcggcgc catgtacttg gaaatggctg
     3241 aacctgtcca atctctgatg gcggtaaaaa gtctggcagg agcagtggta gaagccagca
     3301 aatctatgaa ataccaaggg ctcttcttgg aaaaagaaag cagttattta ggcgaatttt
     3361 ataacaaaaa caaggcgaat caactgacag atgaggaatt tcagctccta ctggactaca
     3421 atgcctatct ttacaagaaa gctgctgaga agattttagc aggccggttc gccatcaatc
     3481 cttatactga aaatggcaga agcattgccc catacgtcca gcaacatcag gctattacag
     3541 gctttgaagc caattaccat ctgggccaag cccgtttcct agaaaagttg gacctagctg
     3601 atggcaagcg tctggtcgga gaaaaactca agcaagcttg gcttgaaaaa ataagagagg
     3661 agttgaatcg atgaagctta ttcccttttt aagtgaggag gagattcaaa aactgcaaga
     3721 agcagaagca aattcgagca aggaacagaa gaaaactgcc gagcaaatcg aagctatcta
     3781 cacttctggc cagaatatcc tggtctcagc atcggctggt tctggaaaga cctttgtcat
     3841 ggcagagcgc attctggacc aattggcgcg tggtgtcgaa atttctcaac tctttatctc
     3901 aacctttacc gtcaaggctg caaatgaact taaagaacgt ttagagaaaa aaatcagcaa
     3961 gaaaatccaa gaaacagatg atgtcgacct caaacaacac ttgggacgcc agttggcaga
     4021 cctaccaaac gctgccatcg gaaccatgga ctctttcaca caaaaattcc ttggcaaaca
     4081 tggttatctg cttgatattg cacctaattt ccgtatttta caaaaccaaa gcgagcaact
     4141 tcttctcgaa aacgaagtct ttcatgaggt ctttgaagcg cattaccaag gtaaacagaa
     4201 agagaccttt agtcatttgc tgaaaaactt tgctgggcgt ggcaaggacg aacggggtct
     4261 gcgccagcag gtctataaaa tctatgactt cctccaatcc accagtaatc ctcaaaagtg
     4321 gctgagtgaa tctttcctca aaggctttga gaaagctgat tttaccagtg aaaaagaaaa
     4381 actgactgag caaatcaaac aagccctttg ggatttggaa agctttttcc gttaccatct
     4441 ggataacgat gccaaggagt ttgcaaaggc tgcctattta gaaaatgttc agttaattct
     4501 ggatgaaatt ggctccctaa atcaggagtc cgatagtcag gcttatcagg cagtgcttgc
     4561 gcgtgttgtc gccatctcta aggagaaaaa cggtcgagct ctgactaatg ccagtcgtaa
     4621 ggctgatttg aagcccctgg ctgatgccta caacgaagag agaaagaccc agtttgctaa
     4681 actaggacaa ttatcagacc agataaccat tctcgactat caagaacgtt atcatcaaga
     4741 cacatgggaa ctagctaaaa ccttccaaac ctttatgagt cattttgtgg aggtttatcg
     4801 tcagcgcaaa cgccaggaaa atgcctttga attcgctgat atcagccatt acaccattga
     4861 gattttagag aatttcccac aagttcgtga gtcttatcag gagcgcttcc atgaagtcat
     4921 ggtcgatgag tatcaggata ccaaccatat tcaagaacgg atgctggaat tgttgtctaa
     4981 tggccacaat cgctttatgg tgggagatat caagcaatcc atctatcgtt tccgtcaggc
     5041 agacccgcag attttcaatg agaaattcca acgctatgcg caaaatcccc aagaaggcaa
     5101 gctcattctc ctcaaggaaa atttccgtag tagttcagaa gtgctgtcag caaccaatga
     5161 tgtctttgaa cgtctcatgg accaagaggt cggcgaaatc aactatgata acaagcacca
     5221 gcttgttttt gccaatacca aactgactcc caatccagac aacaaggcag aatttctcct
     5281 ctacgacaag gacgatacag gtgaggaaga agagagtcaa acagaaacga aactaacagg
     5341 cgaaatgcgc ttagttatca aggagattct gaaacttcat caagaaaaag gtgttgcctt
     5401 taaggaaatt gcccttctga cctccagccg cagtcgtaat gaccagattc tcctcgccct
     5461 gtctgagtac ggaattcctg tcaaaactga cggagagcaa aacaattatc tccaatccct
     5521 agaagtgcaa gtcatgctag acactcttcg tgtcattcac aatcccctgc aagactacgc
     5581 cttggttgcc cttatgaagt ctccaatgtt tggttttgat gaggatgagc tagcacgttt
     5641 gtcccttcag aaagcagagg ataaagtcca cgaaaatctc tatgagaaac tggtcaatgc
     5701 acaaaaaatg gcaagtagtc aaaaaggctt gattcacaca gctctagctg aaaaactaaa
     5761 gcaattcatg gatatcctag cttcttggcg cttgtatgcc aaaacccatt ctctctatga
     5821 cttgatttgg aagatttaca acgaccgttt ttattatgac tatgttgggg ctttgccgaa
     5881 tggtcctgct aggcaggcca atctctatgc cctagcactg cgtgctgatc aatttgaaaa
     5941 gagcaatttc aaaggtttgt cgcgttttat tcgtatgatt gaccaagtct tagaagccca
     6001 gcacgatttg gcaagcgtag ccgtcgcacc gccaaaagat gcagtagagc tcatgaccat
     6061 ccacaagagt aaagggctgg agtttcctta catctttatc ctcaatatgg atcaagattt
     6121 caacaagcaa gactctatgt cagaagtcat tctcagtcgt cagaatggtc ttggtgtcaa
     6181 atatattgcc aagatggaga caggggcagt agaagaccac tatcctaaaa ccatcaaact
     6241 ctccattcct agtctgacct ataggcagaa cgaagaggaa ttacagctag caagctattc
     6301 tgagcagatg cgtttgctgt atgttgctat gacgcgggct gagaaaaagc tctatcttgt
     6361 cggcaagggt tctcgtgaaa agctggaatc caaggaatac ccagcagcca aaaatgggaa
     6421 actaaatagc aatactagac tgcaagcacg gaatttccaa gattggcttt gggctatcag
     6481 taaagtgttt actaaggaca agctcaactt tagttatcgt tttattggcg aagatcagtt
     6541 gaccagagaa gctatcggag agttggaaac caagagtcct ctccaagata gctcccaagc
     6601 agacaatcgt cagtcagata ccatcaaaga agctctggaa atgctgaagg aggtggaagt
     6661 ttataatact cttcaccgcg cagctattga acttcctagt gttcaaaccc caagtcaaat
     6721 caagaaattc tacgaaccag ttatggatat ggaaggtgtc gagattgctg gtcaaggtca
     6781 gtcagtaggc aagaaaatca gcttcgattt gccagatttt tcaaccaaag aaaaggtaac
     6841 tggagctgag attggtagtg ctactcacga actcatgcag agaattgacc tcagccagca
     6901 actaaccctt actagcctaa cagaaacact caaacaagtt caaactagcc aagctgtcag
     6961 agacaagatc aatcttgata aaattcttgc tttctttgac acagcactcg gtcaggaaat
     7021 tcttgctaat accgaccatc tctatcgcga gcaacctttc tccatgctca aacgagacca
     7081 aaagagtcag gaagactttg ttgtccgtgg tatccttgat ggctatctgc tttacgaaaa
     7141 caaaattgtt ctgttcgact acaagacaga ccgctatgat gaaccaagtc aactcgtaga
     7201 ccgctatcgt ggtcagttag ctctatacga agaggcttta tcacgagcct attcgattga
     7261 aaatattgaa aaatacttga ttttactcgg taaagacgag gttcaagttg taaaagtata
     7321 acttatactc ttcgaaaatc tcttcaaacc acgtcagctt cgccttgctt tatatatggt
     7381 tactgacttc gtcagttcta tccacaacct caaaacagtg ttttgagctg actgcggcta
     7441 gctttctagt ttgctctttg atttttattg agtattagaa aggagacctc atgacacttc
     7501 cagttagaaa atccctgcac gatgcagttt tacaggcttc gaaagcggat acttgggaac
     7561 aagctaccaa ggaatggaat gaagtttcct tgatttttaa cggtatcggc cgtagcaatt
     7621 gtgtctgtgg aaatgccatc aaatacgctt atgaactctt taacggtgtc acaggccaac
     7681 gcctctttcc aattggtagc gactgtgttc gtcattttca tcgattgtcc cttgaccagc
     7741 aattggaaga ggaagaaaaa ctgctcagaa aggttgaaaa tctaaccaga aaagctcaga
     7801 aaaaggaaaa aatcaaggtc aataaaagtg actttgacga gcgacttcta aaatggctct
     7861 gggaaaaagg tgttttcaaa cccaatcgtg gcaatcaatt tgcgcctgag agagactacc
     7921 agcttttcct agaagtcttt cagggaggaa gttggaccaa ggcggaacca aagaaaaagg
     7981 ctcggatgga agaagtcctt gaaaagtgta tcaaaccctt tttacttggg aaatcggatg
     8041 accaactcta ccttgtcaag ctaggcaagg agaaaattga ctacgaacaa gaactccgta
     8101 tccaggcaga gaaagaacgc aagaagaggg ataaaatcgc caagcaatat gcagacaatc
     8161 tggttctagc catgggaccc gcagagcgtg cttatcaaga ttactttggc tttacagaga
     8221 ccctgactca agaagaacgc aagtgggaaa aaattctttt tggtaaaaat agagcagaac
     8281 gggatatcaa ggccaaacaa taccaaaaag agctggaaaa agaccaacga attgccagtc
     8341 aggatccaat tgaaagaaaa cagaagcaga cttggctcct caattcctat tttcgtgagc
     8401 ttcctgaaga aaaagctcga ttttctaggc tcttattaga atatcgaaaa agtggtgaag
     8461 tacccttttc aactgaatac ctgtccgatc atctcatcga ctttttctat aaaatgaaag
     8521 cctttgaatt tgaaatcgca ccagaacaag tccgagattt tctaaaaaaa agcctgcagg
     8581 aagaccatcg ctcatcagca caaggaagct ggatagaagg aattctcctc aactgcctca
     8641 aaccattttt agaacgatta gtgatatgag taacacctac cgtgtaaatt tcatggtagg
     8701 tgttattgta cctcaaaata atataaaaca aagtatgaaa gcgcatctca attttaagac
     8761 ttttttgtaa attaagagta taattaaggt atactgcctt t
//
LOCUS       DAVLRT010000075         8748 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLRT010000075 DAVLRT010000000
VERSION     DAVLRT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.075"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            68..1129
                     /gene="dinB"
                     /locus_tag="VXM85_001720"
     CDS             68..1129
                     /gene="dinB"
                     /locus_tag="VXM85_001720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1346266.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(1276..2121)
                     /locus_tag="VXM85_001721"
     CDS             complement(1276..2121)
                     /locus_tag="VXM85_001721"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1346267.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2377..3167
                     /locus_tag="VXM85_001722"
                     /pseudo
     CDS             2377..3167
                     /locus_tag="VXM85_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3546..5435)
                     /locus_tag="VXM85_001723"
     CDS             complement(3546..5435)
                     /locus_tag="VXM85_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1346268.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRIDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSICFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            5573..7138
                     /locus_tag="VXM85_001724"
     CDS             5573..7138
                     /locus_tag="VXM85_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1346269.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            7138..7878
                     /locus_tag="VXM85_001725"
     CDS             7138..7878
                     /locus_tag="VXM85_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1346270.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(7987..8202)
                     /locus_tag="VXM85_001726"
     CDS             complement(7987..8202)
                     /locus_tag="VXM85_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346271.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         2772 a         1905 c         1603 g         2468 t
ORIGIN      
        1 tttgaggttg cagatagagc tgacgtggtt tgaagagatt ttcgaagagt ataaaaaggt
       61 gctaggcatg ttgatttttc ctttgttaaa tgatttgtca agaaaaatca tccatattga
      121 catggatgcc ttttttgctg cagtggaaat cagggataat cctaaactca gaggaaaacc
      181 tgtcattatt ggaagcgacc ctcggcaaac aggtggacgg ggagtcgttt ctacctgtag
      241 ttatgaggca agagcttttg gtgtccattc tgccatgagt tccaaggaag cttatgaacg
      301 ttgtccccag gctgtcttta tctcagggaa ttatgagaaa tacaagtctg tgggactcca
      361 gattcgagct atttttaagc gctatacaga tttgattgaa cccatgagca ttgacgaagc
      421 ctatttggat gtgacagaaa ataaactcgg tatcaagtca gcggtcaaaa ttgctcgcct
      481 cattcaaaaa gatatctggc aagaactcca tctaactgct tccgcaggcg tttcttacaa
      541 caaattctta gctaaaatgg cgagtgatta tcaaaaacca catggtttga cagtgattct
      601 acctgaacag gctgaggatt ttctcaaaca aatggatatt tccaaatttc atggagtagg
      661 aaaaaagaca gtagaacgtc ttcatcaaat gggcgttttt actggtgctg atttacttga
      721 agttcctgag gtaaccctaa tagaccgttt tggtagacta ggctatgatc tgtatcgaaa
      781 ggctcgtggc attcacaact ctccagtcaa atccaatcgc atccgtaaat caatcggcaa
      841 ggagaaaacc tacgggaaga ttctccttgc tgaggaagat atcaaaaaag agctgactct
      901 tctatcagaa aaagtcgctc tcaatctaca tcaacaagaa aaagctggaa aaattgtcat
      961 tttgaaaatc cgctacgagg acttttcaac tcttaccaaa cgaaaaagtc ttgctcaaaa
     1021 aacacaagat gctagtcaga taagccaaat agccctgcaa ctctatgaag aattaagcga
     1081 gaaagaaaga ggtgtccgcc tattggggat taccctgact ggattttaaa gctcaatgaa
     1141 aatcaaagag caaactagga agctagccgc aggctgctca aaacactgtt ttgaggttgc
     1201 agataaagct gacgcggttt gaagagattt tctaagagta taaaaccttg aagagttgcc
     1261 ccttcaaggt ttttcttata caaataaacg gacaaaactg taaagtagca aaacactacc
     1321 aagcacgata cggtatttac caaaaagggt gaagtcgtgt tttttcacat agctggtcaa
     1381 gaagcgaata gccaccatgc tgaccgcaaa agctactccc atcgcgacca agagcaaaaa
     1441 caattgccca aagctcaaga gttctccggc tttcacaaat ttgaaaatct ttaaggcact
     1501 agctccaaac ataacgggaa tcccaagata gaaggtaaat tctgtcacaa ctgaacgact
     1561 ggttccattt aacaaaccac cgacaatcgt tgcacctgaa cggctagtcc ctggtaaaag
     1621 agcaagaact tggaagagtc cgatatagaa agcggtcgta taaggaagct tgtccaactc
     1681 tgttacactt ggctcgatag cacgcgcttt attgcgcttt tccaaatata tgaaggcaac
     1741 cccgtagata atcaacatga gagcaactga aaccatgtta tggaagtggg tatcaaacca
     1801 atcatcaaat ttaaagacac caagtaaagg taaagtagca atcaagacct tcaaccatag
     1861 tctccaagtc ttacgaactt cctgtttgtc cttagtcggt ttaaaaggat tgagcttgtt
     1921 aaaataaatc accataactg ctaaaatagc accaagctga atcacgacat taaacatgga
     1981 cataaaggct tcattttgat tttggtattg gataaactcc tctgctaaaa tcaagtgacc
     2041 tgtactggaa atcggcaacc attccgtaat tccttcaaca atcccgaaga agatagattt
     2101 taaaatttca ataagataca tatatttctc cttatattat atttaacgca gttagctact
     2161 gccaaagccc aagtggtata cttggaataa gccactgtgg attagttcat ttcctccaat
     2221 cgcttgacga tttccgaaag ggtctaacaa cagctttttg tttcaattgg taatgagttt
     2281 gataacgcat agacgtttta tctcgtctaa ggtaacaagg caaaaagttc gtggataacc
     2341 aagtagactt gaaatactat atctggaggt actcccatgc tttatgtggg cattgatgtc
     2401 gctaaaaata aacacgatgt tacagccttg aatgttcgag gaaaactgtt cttaaaccac
     2461 tcactttttc aaataataaa gctggttttg aactcttaga tctgtctttt cgacagctca
     2521 accaagactg tctcatcgct cttaaacttc tttctgaccc caatcgtgaa caatttcaac
     2581 acgataatcg gcaaatagaa ctaaaaatac tggctagaca tattcatcgt ctcaagaaaa
     2641 aacagtctga ttggaaagta caatacactc gttgtcttga tatcatcttt cctgagttgg
     2701 ataaaatcgt tggaaagcat tcagaatata cctaccaact cttgacacgc taccctaatc
     2761 ctcagaaaag gcttgaggca ggatttgata agctgataga aattaagcga ttgaccgctt
     2821 ctaaaattca ggatatcctc tcagtcgcac ctcgttctat cggaacaaca tctcctgctc
     2881 gtgaattcga aatcatcgaa atcatcaaac attacaagag gctcattgac aaggcggaaa
     2941 catgtgtcaa tgacttgatg gctgagttca actcggtcat cacgacggtt actgggattg
     3001 ggggtcgttt aggggcggtc attttagccg agattcgaaa tattcatgcc tttgataatc
     3061 ctgctcaatt acaagctttc gctggactgg attcttctat ttatcagtca gatcagattg
     3121 atttagcagg aagaatggtc aaacggagtt cccctcatct gcggtaggta ctcatacaag
     3181 ctgccaaagc atgccctcgc ttttcacctg ctttcaaggc ctatcttaag actaagttag
     3241 aacaaggaaa acattacaat gtagccatca tccaccttgc aaaaaaactt atccgaaccc
     3301 tgttttatat cctaaaaaag agctgccatt tgacgaacaa aaagtgatgt aatttctgaa
     3361 attaacacag gattaccttt gaaaaaatgg actttttcaa gggctttttg ccgtgctcta
     3421 aaatcatcgc caaatcatca aattgaaata agaaatagtt tagataaaac tattgacttt
     3481 tcttatagtt tgtctttctt ctatcttcca ttatatcata ctttttcagt ctatggtagc
     3541 tctctttaaa aggctccgat actgccgcca cctccgcctc cagagaagcc accgccagaa
     3601 cttccacttc cagaagatac ggagtaggta cttgctgtat ttgcgacact agcataatgg
     3661 ctcatttgcg cgcttgaatg ataaaacata ctgtgccagc cataagctac atagagattg
     3721 atatctggat tttcaacttg aatctgatga accttcatca aatgactaac cttgtccgca
     3781 tagccaaata aggttgcata gaccaagaga cgattccaaa caacaatact ttccaattca
     3841 gccttatcca gtcgtgcaat ctcacgtaac atattttcaa aactggtcca gagatagtag
     3901 acctcagctc ccgcttcatt tagaacacca tcacgattat ctagtcgaag cttccaataa
     3961 tagaaaacag acaaaactaa ccctagaaaa ccaagtattg gcaaagggag gtaaagatag
     4021 ccatgaacgt ctaaactgta caagaacaaa ccaaatccga taaatagggg caagatagtc
     4081 aagacaccca tacccacttg caaagtcttt tccaaaccag ttaaaggacg atagtaatct
     4141 gggagccccc agaaggaaac tcgttttctc actccttctt gcatctggtt caatacctct
     4201 tcaaaagaag atttgagttg aagccctctt gcttgaatcc gtttttcatc agaaactttg
     4261 gctctacgat aaagactatc agataccttg taatccgcaa acaaattgga aagagtggct
     4321 tcttttttgc ctgaaaaggc tagatttaga cagtctctct caaatcttga caaaccatct
     4381 tctttcacca atctcaagcc aactgcatct ccttctgaaa taatagagac attcccacgg
     4441 tctatcacat ctagcaaggt agcttgaata agttgatcaa aggtgaattt tccagctccc
     4501 ttgaccaagg gactcacttc ctccaaggag gtcgagtaga ctgcttctga taaaaccata
     4561 ggctctaatt ccattggtgg ttcatagaga cgatgatttt tggcatattt gactgaagga
     4621 gtggtctttc ttctataaat aaaatagaag cagatgctca ataacaagga gatggaaagg
     4681 atcgaaggga ggacccaagt aacgagttgt ttactctgat ctttttctct aacaatcgag
     4741 tcttctatct tattaaactc ttctaaacga ttccctttca agccctgatc cctagcgcta
     4801 gcaaaatcga tccgagacca ataggcatgc aactcaactc cacgcttagc cggaagattg
     4861 tctaaacgga tagtataatc aaggttactc ttttcaatcg ttccctctct aaaaagtttc
     4921 cctgtatgga aaaagagttt ttcagccccc ttgtctcccc ttacatgaaa ttcaaacttt
     4981 tcaatagact ctgaactatc tgtcagaggt tgccaattta attcagcgat atcatcataa
     5041 aggaaaagta aattttttaa gttccagacg aggtcaactt caactatgtc gccctcctga
     5101 cctggattat agactctcac agtataacca tccgcttctt ctgttacttc gctagtcaca
     5161 tctgctagtt ctgcaccgtt tttcgcagcc tgaacctttg gatgagggtc aatgtcaaac
     5221 ccgctaggca tcttaccagc acgtccaagt cccacgattt ggcccttaaa gtcctcctca
     5281 aactggtaaa ctatcttctg tctaaactct gccgtattat ctgcatgaat atacaaatcc
     5341 ccttgataag agtttatctt gaaatcaatg gcaaaaacag agagtggaag aaggcaaaac
     5401 aagcctaaca ccagtaagaa aaaagttttt ttcatcaact aagccccctt tttatctttc
     5461 ttatcattat agcatctttt cgtaagtaaa ggcttactta tgttgaaaaa ttacgcattt
     5521 ttcgataaaa taggagacag ttatttttta atagatcaga ataggagaaa tcatgagaaa
     5581 aatatactta tctattttca caagtctctt gctgatgcta ggacttgtca atgttgctca
     5641 agccgatgaa tatttacgca tcgggatgga agcagcatat gctcccttta actggaccca
     5701 ggatgatgat agcaacggag ctgtcaaaat cgatgggacc aatcagtatg ccaacggata
     5761 cgatgttcaa atcgccaaga aaatcgctaa ggacttaggt aaagaacctt tggttgttaa
     5821 aaccaagtgg gaaggtctag tccctgccct tacttctggt aagattgaca tgattatcgc
     5881 aggtatgagt ccaactgcag aacgcaaaca agaaattgcc ttttcgagca gttactacac
     5941 tagcgaacca gttttgcttg tcaaaaaaga ttctgcctac gcaagtgcta aatctttgga
     6001 tgactttaat ggtgcgaaaa tcacttctca acaaggtgtc tacctctacg acttgattgc
     6061 acaaatccca ggtgctaaaa aagaaacagc catgggagac ttcgctcaaa tgcgccaagc
     6121 tcttgaggct ggtgtcattg atgcttatgt ttctgaacgt ccagaagcac tgactgctga
     6181 agctgcgaac tctaagttca agatgattca agtagaacct ggtttcaaaa ctggggaaga
     6241 agatacagct atcgctatcg ggcttcgtaa aaatgacaat cgtattagcc aaatcaatgc
     6301 cagcattgaa accatttcaa aagatgacca agttgccttg atggatcgta tgatcaagga
     6361 acaacctgcc gaagctacaa caactgaaga gactagcagt agtttcttta gccaagtcgc
     6421 taaaattctt tctgaaaact ggcaacaact cttgcgtggt gctggtatca ctcttttaat
     6481 ctctatcgtc ggaaccatca taggtctcat tattggactt gccattggtg tcttccgtac
     6541 tgctcctctc tctgaaaaca aagccattta tggccttcaa aaactagtcg gctgggttct
     6601 caatgtctac attgaaattt tccgtggtac gccaatgatt gttcaatcga tggttatcta
     6661 ctatggaact gcccaagctt tcgggatcaa ccttgaccgt acactggctg ctatcttcat
     6721 cgtttcaatc aataccggtg cctacatgac tgaaatcgtc cgtggtggta tcctagcagt
     6781 tgacaaggga caatttgaag ctgcgactgc tcttggtatg acccataacc agaccatgcg
     6841 taagattgtc ctacctcagg tagtccgtaa catcctacct gcaactggta atgaatttgt
     6901 catcaatatc aaagatacat ctgtattgaa cgttatctct gttgtcgaac tttatttctc
     6961 aggaaatacc gtggcaacac aaacctatca atacttccag acatttacaa tcatcgccgt
     7021 gatttacttt gtcctcacct tcaccgtaac acgtatccta cgatttatcg agcgccgaat
     7081 ggacatggat acctatacta caggtgctaa ccaaatgcaa acggaggatt tgaaataatg
     7141 acacaagcaa tccttgaaat taaacacctc aaaaaatcct atggacaaaa cgaagtgcta
     7201 aaagacattt cactcactgt ccacaaggga gaggtcatct ctatcatcgg aagctctgga
     7261 agcggaaaat cgaccttcct acgctccatt aacctacttg aaacaccaac tgatggacaa
     7321 atcctttatc atggacaaaa cgtcctcgaa aaaggctatg acctcacgca ataccgtgaa
     7381 aagttgggga tggttttcca atcctttaac ctctttgaaa atctcaatgt tcttgaaaac
     7441 acaatcgtcg ctcagacaac tgtcctaaaa cgcgaacgca cagaagctga aaagattgcc
     7501 aaagaaaacc tggaaaaggt cggcatggga gaacgctact ggcaagccaa accaaaacaa
     7561 ctctcaggtg gtcaaaaaca acgtgtggcc atcgctcgtg ccctctccat gaatccggac
     7621 gctattctct ttgatgaacc aacatcagct ctcgatccag aaatggttgg agaagtcctc
     7681 aaaatcatgc aggacctggc tcaggaaggc ttgaccatga ttgtcgtaac ccatgaaatg
     7741 gaatttgccc gtgatgtctc tcaccgtgtt atctttatgg ataagggcgt gatcgctgaa
     7801 gaaggtaaac cagaagacct cttcaccaat cctaaagaag accgaacaaa agagttcctt
     7861 caacgctatc tcaaataaaa agaaaaggct gcatcaaccg tgcagtcttt ttgctgtcct
     7921 caaaatgaaa aggcagattc tctatctcta ctacttgaag gtcaagaatc cgccgttatc
     7981 caaagattaa tcttcaaatt tttcataatt tttttcatag aaatcaatta ggcctagggc
     8041 tgtttcaaat gaaatatttt ttacttttgc acgaccctgc gccagagcaa tgatagacat
     8101 ttcacgcgca ttcgtttctt tagagatacg gtagcctgtg attttcttat cacgaaccca
     8161 gccaacaact gattctactt tttcaaagtt tgacttagcc atgtttttct cctattttct
     8221 tctttataat atttaattgt atacgttttt actacttttg tcaattaaaa aaacatatat
     8281 tcaacgaaaa taattagttc agaattttct tacttccttt ttaaatccga taatatatag
     8341 gttttgctag cctctacacc ataaatattt gctaataaaa atactgtatt attaccctct
     8401 taaggtgcga aattattcaa gttcttaaaa aatgcttctc tatttccgta agatagctta
     8461 tttcttcgat ttaagaactg aagatgatag ttctaacctt tagaaatcca agccttcact
     8521 agctcaaaaa ctcctatctt tcttcctcaa taaatcatct gagagatagg tattccttat
     8581 gtccttcaaa ttcatcactc ctttggatat tgaggtgcta gcctagaagc agacacccga
     8641 ttatctaaac catccttaat agagatgaca agacagataa gctcagttaa ttacttatat
     8701 atctttttaa tatagtatat ttgtcaacaa aaagaaatca aatcatac
//
LOCUS       DAVLRT010000076         8718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLRT010000076 DAVLRT010000000
VERSION     DAVLRT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.076"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            91..3192
                     /locus_tag="VXM85_001727"
     CDS             91..3192
                     /locus_tag="VXM85_001727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1346272.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQVDD
                     SKKKLDVTLFSDLYRQVDQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSDQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            3275..4282
                     /gene="pfkA"
                     /locus_tag="VXM85_001728"
     CDS             3275..4282
                     /gene="pfkA"
                     /locus_tag="VXM85_001728"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1346273.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4341..5846
                     /gene="pyk"
                     /locus_tag="VXM85_001729"
     CDS             4341..5846
                     /gene="pyk"
                     /locus_tag="VXM85_001729"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1346274.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6071..6364)
                     /locus_tag="VXM85_001730"
                     /pseudo
     CDS             complement(6071..6364)
                     /locus_tag="VXM85_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(6530..6755)
                     /locus_tag="VXM85_001731"
                     /pseudo
     CDS             complement(6530..6755)
                     /locus_tag="VXM85_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7603..8475
                     /locus_tag="VXM85_001732"
     CDS             7603..8475
                     /locus_tag="VXM85_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1346275.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8608..8718)
                     /locus_tag="VXM85_001733"
                     /pseudo
     CDS             complement(8608..8718)
                     /locus_tag="VXM85_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2656 a         1522 c         1888 g         2652 t
ORIGIN      
        1 attagtaagc tgatgaaatt tttctcaaga gaagccattt ataggtgaaa atggtataat
       61 atagtgagaa ggatagagga gaagtgtaaa ttgatcgcac aactagatac aaaaacagtc
      121 tatagtttta tggaaagcgt catttcgatc gaaaagtatg tgagagcagc taaagaatac
      181 ggctacactc atttggctat gatggatatt gacaatcttt atggcgcttt cgactttcta
      241 gagattacaa aaaaatacgg cattcatcct ttgctagggc ttgaaatgac agtgtttgta
      301 gatgatcagg aagtgaattt gcgtttttta gctctatcta gtgtgggcta tcagcagttg
      361 atgaagcttt cgacagccaa gatgcagggg gagaaaactt ggtcagtcct gtcccagtac
      421 ctggaggata tcgcggtcat tgtgccttat tttgatagag ttgagtcgtt agaactaggc
      481 tgtgattact atataggggt ttatccagaa acactagcaa gcgaatttca tcatcctatc
      541 ttacctcttt atcgggtcaa cgcttttgaa agcagggata gagaagttct tcaagtttta
      601 acagcgatta aagaaaatct accgctcaga gaagttccct tgcgttcgag acaagatgtc
      661 tttatatcag caagttcttt agagaaacta ttccaagagc gttttccgca agctttggac
      721 aatttagaaa agcttatttc aggcatttct tacgacttgg atactagtct gaaactgcct
      781 cgttttaatc cagctagacc agcagtagag gagttgagag agcgtgctga actggggctt
      841 gttcagaagg ggttgactag taaagaatat caagatagac tagaccaaga attgtctgtt
      901 attcatgata tgggctttga tgattatttc ttggttgttt gggatttgtt gcgttttgga
      961 cgatcgaatg gctattatat gggaatggga aggggttctg cagtaggcag tttggtttct
     1021 tatgccttag acatcacggg gattgaccca gtagagaaaa atctgatttt tgaacgcttt
     1081 cttaatcgtg aacgctatac catgcctgat attgatattg atattccaga tatttatcgt
     1141 ccagatttta tcagatatgt tggtaataaa tatggtagta aacatgcggc acaaatcgtt
     1201 actttttcaa cctttggagc caagcaagct cttcgagatg tcttgaaacg ctttggtgtg
     1261 ccagagtatg aattatctgc aattactaag aaaatcagtt ttcgtgacaa tcttaagtcg
     1321 gcctatgagg gcaatctcca gtttcgtcag caaatcaata gtaagttaga ataccaaaaa
     1381 gcttttgaga ttgcttgcaa gatagagggc tatccaaggc aaacctctgt ccatgcggct
     1441 ggtgttgtaa ttagtgacca agatttaacc aactatattc ctctaaagta tggtgatgaa
     1501 attccactga ctcagtatga tgctcatgga gttgaggcta gcggactttt gaagatggac
     1561 tttctgggac tacgaaattt gacctttgtc cagaagatgc aagagttgct tgctgaaata
     1621 gaaggtattc accttaaaat tgaagaaata gatttggaag acaaagaaac gttagattta
     1681 tttgcctctg gaaatacaaa aggtatcttt caatttgagc aaccaggtgc cattcgtctg
     1741 cttaagcgtg tgcaaccagt ctgttttgaa gatgtcgtcg cgactacttc tctaaatcga
     1801 ccgggtgcta gtgactatat caataatttt gtggcaagaa agcatgggca ggaagaagtg
     1861 actgttctgg atccagtact ggaggatatt ttggctccaa cctacggcat aatgctctat
     1921 caggagcagg ttatgcaggt tgcccagcga cttgccggat ttagtcttgg gaaagccgat
     1981 attttgcgtc gggctatggg gaaaaaggat gcctctgcca tgcatgagat gagggcttcc
     2041 tttattcaag gttcattaga agctggtcat actgtggaaa aagcagagca ggtctttgat
     2101 gttatggaga agtttgcagg ttatggtttt aacaggtcac acgcctatgc ctactcagcc
     2161 ttggccttcc agttggctta tttcaaaacg cattatccag ccatttttta tcaggtcatg
     2221 ttaaattctt ccaacagtga ttacttaata gatgcacttg aagcaggttt tgaagtagcc
     2281 tctctatcca tcaacaccat tccctatcac gataaaattg ccaacaaggc catctatcta
     2341 ggtttgaaat ccattaaagg agtcagtaat gatttagctc tctggattat tgaaaataga
     2401 ccttattcta acattgaaga ttttatagct aaattacctg agaattatct gaaacttcct
     2461 ctgctagaac ctttggtaaa agttggtctt ttcgattcat ttgaaaaaaa tcgtcaaaaa
     2521 gtatttaata acttagctaa tctatttgaa tttgtgaaag agttgggaag tttgtttgga
     2581 gatgctattt atagttggca ggaatcggaa gattggacgg aacaagaaaa attttatatg
     2641 gaacaagagc ttttagggat aggtgtcagc aaacatccac tacaagctat tgcaagtaag
     2701 gctatttacc cgattacccc aattggaaat ttgtcagaaa atagctatgc tattatcttg
     2761 gttgaagttc agaagataaa agtaattcgt accaaaaagg gtgaaaatat ggctttctta
     2821 caggtggatg acagtaagaa aaaattggat gttacactct tctcagattt atatcgacag
     2881 gttgatcagg aagtaagaga aggagcattc tactatatca aagggaaagt ccagtcacgt
     2941 gacggtcgtc tgcaaatgat tgcacaagaa ataagagaag cagtggctga gcgcttttgg
     3001 attcaggtta aaaatcatga ctcggatcaa gagatgtcac gtattttaga ccagtataaa
     3061 ggcccaatcc cagtcatcat ccggtatgaa gaggaacaga aaaccatcgt ttctccccat
     3121 cattttgtag ctaaatctga tgaattagag gcgaaattga atggaatagt tatgaaaacg
     3181 atttatcgct aaaaatacgg aaaatagaag aattttaaaa tcaaatgtga tataatcaag
     3241 aagaatgtta aaagaaaaag gagcataaac caatatgaaa cgtattgctg ttttgactag
     3301 tggtggagac gcacctggta tgaacgctgc tatccgtgca gttgttcgtc aagcaatttc
     3361 agaaggaatg gaagtgtttg gtatctatga cggatatgct ggtatggttg ccggtgaaat
     3421 tcacccccta gatgcagctt cagtaggaga catcatttct cgtggtggta cttttcttca
     3481 ctcagctcgt tatccagagt tcgctcaact tgaagggcaa cttaaaggga ttgagcaatt
     3541 gaaaaaacac ggaattgaag gtgtagttgt tatcggtggt gacggatctt accacggcgc
     3601 tatgcgtttg actgaacatg gcttcccagc tattggtctt ccaggtacaa tcgataacga
     3661 tatcgttggt actgacttta caatcggttt tgacacagcg gttactactg ccatggacgc
     3721 tatcgataag attcgtgata catcatcaag tcaccgtcgt acttttgtaa tcgaagttat
     3781 gggacgtaac gctggtgata tcgctctttg ggctggtatt gcaactggtg ctgatgaaat
     3841 catcatccct gaagcaggct tcaagatgga agatatcgta gcaagcatca aagctggtta
     3901 tgaatgtggt aaaaaacaca atatcatcgt cttagctgaa ggtgtgatgt cagcggctga
     3961 atttggtcaa aaacttaaag aagctggaga tacaagcgac cttcgtgtaa cagaacttgg
     4021 acatattcaa cgtggtggtt ctccaactgc gcgtgaccgt gttttggcgt cacgtatggg
     4081 tgcacatgct gttaaacttc ttaaagaagg tatcggtggt gttgcggttg gtattcgtaa
     4141 cgaaaaaatg gttgaaaatc caattcttgg tactgcagaa gaaggggcat tgtttagcct
     4201 tactgcagaa ggtaagattg tggttaacaa cccacacaaa gccgatattg agctatctag
     4261 cttgaataag agcttgtcat aatttacaat ttagttaata agaccaaaaa ggtcatatat
     4321 ataaaggagt cacaaaaatc atgaacaaac gtgtaaagat cgttgcaact ttgggtcctg
     4381 cggtagaaat ccgtggtggt aaaaaattcg gtgaggatgg atactggggt gaaaaacttg
     4441 atgttgaagc ttcagctaaa aacattgcta aattgattga agctggtgct aacacattcc
     4501 gattcaactt ctcacacggc gaccaccaag aacaaggtga gcgtatggca actgttaaac
     4561 ttgcggaaaa aattgcaggt aaaaaagttg gtttccttct tgatacaaaa ggacctgaaa
     4621 tccgtacaga attgttcgaa ggtgaagcta aagaatattc atacaaaact ggtgaaaaaa
     4681 ttcgtgttgc aactaaacaa ggaatcaaat caactcgtga agtgattgcg ttgaacgttg
     4741 ctggtgctct tgatatctat gatgatgttg aagttggtcg tcaagttttg gttgacgatg
     4801 gtaaacttgg tcttcgtgtg gttgctaaag atgatgcaac tcgtgaattt gaagttgaag
     4861 ttgaaaacga tggtatcatc gctaaacaaa aaggtgtgaa catccctaac actaaaattc
     4921 ctttcccagc tcttgctgaa cgcgataacg acgatatccg tttcggtctt gaacaaggta
     4981 tcaacttcat cgcaatttca ttcgtacgta ctgcaaaaga tgtgaacgaa gttcgtgcaa
     5041 tctgtgaaga aactggaaac ggacatgttc aattgttcgc taaaatcgaa aaccaacaag
     5101 gtatcgataa cttagacgaa atcatcgaag cagctgatgg tattatgatt gctcgtggtg
     5161 atatgggtat cgaagtaccg ttcgaaatgg ttccagttta tcaaaaaatg attatcaaga
     5221 aagtcaatgc tgcaggtaaa gttgttatca ctgcaacaaa catgcttgaa acaatgactg
     5281 aaaaaccacg tgcaactcgt tcagaagtat cagacgtatt caacgctgtt atcgacggaa
     5341 ctgacgctac aatgctgtca ggcgagtctg caaacggtaa atacccactc gagtcagtaa
     5401 ctacaatggc tacaatcgac aagaacgctc aagctcttct taatgaatac ggacgtcttg
     5461 attcagattc atttgagcgt aactctaaga cagaagtaat ggcttctgct gttaaagatg
     5521 ctactagctc aatggatatc aaattggttg taactcttac taagacaggt catactgcac
     5581 gtttgatttc taaataccgt ccaaatgctg acatcttagc attgacattt gacgaattga
     5641 cagaacgtgg cttgatgttg aactggggtg ttattccaat gttgacagat gctccatctt
     5701 caactgacga tatgttcgaa atcgctgaac gtaaagcggt agaagcaggt ctcgttgagt
     5761 caggcgatga tatcgttatc gttgctggtg tgccagtagg agaagctgtt cgcacaaaca
     5821 caatgcgtat ccgcacagta cgttaagaaa aatataaaaa cctatcatat ccagctttag
     5881 agcttgtgtg ataggctttt tgtatagagg gtaagaaata ggcaaaactt tcataatgga
     5941 ttgatactct tcgaaaatct cttcaaacca cgtcagcgtc gccttaccgt actcaagtac
     6001 agcttgcggc tagcttccta gtttgctctt tgattttcat tgagtatgaa ataagatatg
     6061 cacaaattga ttagaaagtc aaatgaattt ctacaaatgt tttagcaatc gtaatgtact
     6121 tgtctagatt cgatctgata tattttcgat ttaatgatat ggtatttaaa acctccaaag
     6181 tagcttactc cattctttta cttacgtgag tgtagatgtt atttactgtt ttagcgtttt
     6241 tgtgttccac tctaaccatt atagcattct tctcagctag tgtactaagg agtgtgtgcc
     6301 tgaaaatatg ggaactaagg ggctggttta tcggtttctc tagtttagta tttgcctttt
     6361 gcaaagtgat cttaaatgcc tttctctaaa tttacatatc actattgttt aacaaaatct
     6421 aatctatctt aggtcactta ttcttttttt gaaatgtaga atgaactttt tcaaagtttt
     6481 tcgaatcttt taaaatctgt ttgttttata tcgccattct cccccttttt taattctccc
     6541 tatatagcct gacaactttc ccgatggtac gaatatggtt gctttcgtct aggtggatgt
     6601 cggagtattc gggattgagt ttttttgagg tagccttggc ggagtttctt gacatagtta
     6661 gtgccgtcta cttggaagat gccgatggta ttatagtcaa tctgtggggt attcttgata
     6721 aataggtagt cgctgtttct tatctttggc tccatggact tgctgacgac ataagcgatt
     6781 gggtcgtagt cgtctgggat aatggaaact ccataactaa atcgtcgtcc tgcatcaagc
     6841 ggctacctgc agagataaac tacctaacac gagagtaagt agtctgtctg tagtcgtcca
     6901 gtctgatgat ttttacgata cttcgttttt ctgatcatac agttgcctct cggcataggt
     6961 cagaacttta ccttgtctgg gtggtgcccg ttggtcgtag atagattgga tatcgctagg
     7021 agaatccttt tgaactggag gaaagagggc atcaatcaag ctactgaata ctttaactaa
     7081 gtcaaatata gtatttttct tagtagacct aacccttttt tcataatttc taatggtgtt
     7141 tttacttata cctatcttag tacccaattc ttattgagtc caaccattac tagtctatat
     7201 tgttttatag ttgattgagt ttggaatagt acgctgtagc tgctaaaaca tttctaggaa
     7261 ttaatttgcc tttcctaata gagttgttca tatcttattt caatctacta tatttttcac
     7321 ctctaacaat cgcaatctct tctttatcca tgaacgaaaa cgctttctat ctttgtaagt
     7381 aaagcatcac acgaaatcca cgaaaatgaa aacctttgtt gtgttttcgt aaaaaatttg
     7441 ttgacagagc acgaaacgcc tactataatt aaatcaagtt taaggaaata acaaaaaaac
     7501 aaatcggagg gaaagacgat gaacacatta aatgagaaag taatcaatat ctgtaaagca
     7561 gtagttaaag aaactttaat ccaagacatt taggagaaag aaatgaagaa atcaagaaaa
     7621 ttagccacat taggaatctg ttcagccttg tttttaggct tggctgcttg tcaacaacaa
     7681 catgctactt ctgaggggac gaatcaaagg caaagcagtt cagcgaaagt tccatggaaa
     7741 gcttcataca ccaacctaaa caaccaggta agtacagaag aggtcaaatc tctcttatca
     7801 gctcacttgg atccaaatag tgttgatgca ttttttaatc tcgttaatga ctataatacc
     7861 attgtcggct caactggctt atcaggagat ttcacttcct ttactcacac cgaatacgat
     7921 gttgagaaaa tcagtcatct ctggaatcaa aagaagggcg atttcgttgg gaccaactgc
     7981 cgtatcaata gttattgtct tttgaaaaat tcagtcacca ttccaaagct tgaaaagaat
     8041 gaccaattgc ttttcctaga taacgatgcg attgataaag gaaaggtctt tgattcacaa
     8101 gataaggaag agtttgatat tctattttcg agagttccaa ctgaggcaac tacagatgtc
     8161 aaggttcacg ctgaaaagat ggaaacattc ttctcacaat ttcaattcaa tgaaaaagct
     8221 cgaatgctgt ctgtagtctt gcacgacaat ctggatggcg agtatctgtt tgtaggtcac
     8281 gttggggtct tagtacctgc tgatgacggt ttcttatttg tagagaaatt gactttcgaa
     8341 gagccctacc aagcgattaa atttgctagt aaggaagatt gctacaagta tttaggcacc
     8401 aagtatgcgg attatacagg cgagggactg gctaagcctt ttatcatgga taatgataag
     8461 tgggttaaac tttaatagtg ttatgatatg acagtaatct taggattttt atttctattt
     8521 ttgatgagtt ggtgggttat ttcaatcgtt aatagccaga aaccacgcaa aaatgaaaca
     8581 tttattggct atatccaaag atacgatatt gatgggaatg gctatcaatc attaccgtgt
     8641 cctcagaact gagaggagtt cttgaaatcg taaaactact ttgaacaaga gttacttcaa
     8701 cccattggct ccgacgga
//
LOCUS       DAVLRT010000077         8529 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLRT010000077 DAVLRT010000000
VERSION     DAVLRT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.077"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..1557)
                     /locus_tag="VXM85_001734"
     CDS             complement(<1..1557)
                     /locus_tag="VXM85_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1346276.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRENLSNLRTYRRQNSDNTPRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEERIARIIPLRYRSNHWVPDSRPEEPSPQPAPNPQPAPSNPIDEKLVKE
                     AVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSD
                     REFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVPSDKVKLVDDILAFLAP
                     IRHPERLGKPN"
     gene            complement(1811..3544)
                     /gene="ptsP"
                     /locus_tag="VXM85_001735"
     CDS             complement(1811..3544)
                     /gene="ptsP"
                     /locus_tag="VXM85_001735"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1346277.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(3550..3813)
                     /locus_tag="VXM85_001736"
     CDS             complement(3550..3813)
                     /locus_tag="VXM85_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1346278.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            4164..4382
                     /gene="nrdH"
                     /locus_tag="VXM85_001737"
     CDS             4164..4382
                     /gene="nrdH"
                     /locus_tag="VXM85_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1346279.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            4463..6622
                     /gene="nrdE"
                     /locus_tag="VXM85_001738"
     CDS             4463..6622
                     /gene="nrdE"
                     /locus_tag="VXM85_001738"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1346280.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            6810..7772
                     /gene="nrdF"
                     /locus_tag="VXM85_001739"
     CDS             6810..7772
                     /gene="nrdF"
                     /locus_tag="VXM85_001739"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1346281.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            7954..>8529
                     /locus_tag="VXM85_001740"
     CDS             7954..>8529
                     /locus_tag="VXM85_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1346282.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGE"
BASE COUNT         2485 a         1784 c         1626 g         2634 t
ORIGIN      
        1 atttggtttt cctaaacgtt ctggatgacg aatcggagct aagaaggcaa gaatatcatc
       61 cactaacttg actttatcac ttgggacatc cttgagtcgt tccaacaggt tatccaaagc
      121 ctcaaaatca acttgtcgac ctttattatc aagtaaatct tggtgaattc ttgctagtaa
      181 gtcataagcc ttattgtaaa attctcgatc actagatggg aggtcagttt tcttagctcc
      241 tagcttatga gataaacttt cctgcttggc cagtttgcta tcaatgcctg ctgctgtttc
      301 tgctgaaaga tccttggctg ggatataacg agaaactcca ttctcctcaa agacataacc
      361 atcgcctact tttcgaacag cttctttgac caatttctca tcaattggat tgcttggagc
      421 tggttgagga tttggtgcag gttgtggact tggttcttct ggtcttgaat ctggtaccca
      481 atggtttgaa cgataacgaa ggggaataat acgagcaatt cgttcttcca attcagacat
      541 ttgttcataa gggataaagt ggtaatggtt accatgaggg acagctacac ctctggcggt
      601 tcgacttgtg atttgcgctg ggtcgaaaat aaggccatca gattctacat ggcgttgact
      661 caaaggcagt ttgtagagct gtttcaagag actatcaatg tcattacttt gacttgcttg
      721 actgttagtg ttgctgttgt tgcttgtgtt agtatttgta gttcctggat tgcttacaga
      781 aggtacccag tttgttcttg gagtgttatc gctattttgt cggcgatagg ttcttaaatt
      841 tgacagattt tcccgaccag ataggaaggc ttctgcagca gccaactcgc tagctgataa
      901 ctcattctta ggaatgtaat ggtaatggtc gccgtgagga acgatataag catcacccgt
      961 gtcctcaatg atatcagatg cattgaagat atacccatca tctgtggtgt agcgtccctg
     1021 tgaacgtgca aaggctaccg caccatcgtt tgctgaagtc cctccttcac gatgctgact
     1081 atgttcttgt ttttgccgat tgatttcttc ttttgtacgg acattatccg cgtgggcagc
     1141 atccttaagg taaacatagt attttccatc taccttgata acataaccac ccttgatttc
     1201 actgataata tcctcgtctt ttagctggta gtttggatcc ttcatcagca attcttcact
     1261 gatgatagcg tcataaggaa ccttcccatt gtaataatga tagtggtcgc catgtgaagt
     1321 cacatagcct tggtctgtta tcttgatgac gatttgctca gcattgattc cttcacgctt
     1381 gctaacctca tcaggagtca aattctccgt tttttgcgtc gcttgttttc catctatata
     1441 ggaaacacga ttattttcct taaccgttct agcttgatac agtcccaact cgtaagaaca
     1501 aacacttaaa atcaaagctg ccgcagaacc aacaaggtat ttcttattaa ttttcatcga
     1561 aactctcttt ctatttgata tttctagttg caattcattt ctaaattgca gaaactcctc
     1621 tctattactt aactggttaa tagtttactc ctaaatctcc aacttgtcaa gaattttatg
     1681 aaccatttaa gtattttttg ttctgaccac tactttttca aggttggact ttctataaga
     1741 aagattttct tgaaaattct ttttaaaata tcaaaaaaat ccctgtaact gagttgcagg
     1801 gatttttcga ttaatcaaaa ttaacgtatt ctttttgaag ttcaagaact tcttccattg
     1861 ttgagcattc tgtaagggca cggtttgcgt actcttccat cttagctgtg tcgagtttct
     1921 tcatcaagct gcgtgtacga agtacagatg ttgctgacat agagaactca tccaagccca
     1981 ttccgacaag aagtggaaca gcttgttggt caccagccat ctcaccacac ataccagccc
     2041 atttaccttc agcgtgagct gctttgatca cattgttaat caagcgtagg attgatgggt
     2101 tgtatggttg gtaaaggtat gaaacttgtt cgttcatacg gtctgctgcc attgtatatt
     2161 ggatcaagtc gtttgtacca attgagaaga agtcaacttc tttagcaaat tggtctgcaa
     2221 gcatagctgc tgcaggaatc tcgatcatga taccaacttg gatgttatcc gcaactgcaa
     2281 caccttcagc aagaaggttt gctttttctt catcaaagac tgctttcgct gcacggaatt
     2341 ctttcaagag cgcaaccatt gggaacatga tacgcaattg accgtgaaca gacgcacgaa
     2401 gaagagcacg gatttgtgtg cggaacatag catctccagt ctcagagata gagatacgaa
     2461 gagcacggaa tccaaggaat gggttcattt cgtgaggcat atcgaagtaa ggaagttcct
     2521 tatctccacc gatatccatt gtacgaacga caacaggttt accgttcatt ccttcaagaa
     2581 cagccttgta tgcttcatac tgctcatctt cagttgggaa gtcttgagaa tccatgtaca
     2641 agaactctgt acggtaaagt ccaacagctt ctgcaccgtt gttgttaaca ccttcaacgt
     2701 cttttggagt accaatattg gcagccaact cgaagtgttt accgtcagca gtcactgttt
     2761 gagcatcttt caaaagtgcc cattcagctt tttgtttcgc ataggcttca ccagctgctt
     2821 taaattctgc cgcttgttca tctgttgggt tgataatcac ttctccagtg atcccgttaa
     2881 cagcaaggat gtcaccgtct ttaacgattt cagtgatgtt gtttgtacct aatacagcag
     2941 caatttcaag tgtacgtgcc atgatagctg agtggcttgt acgtccacca atgttggtta
     3001 caaaagcttt tacaaagttt ttgtccaatt gagctgtatc tgaaggagtc aagtcatgcg
     3061 caatcacaat cacttcttca ttgatagaag ctgggtttgg caatttttta ccaagaaggt
     3121 ttgccaatac acgttttgtc acgtcgcgga tatccgctgc gcgttcttgc atgtatgggt
     3181 tgtcttccat gccttcaaag atagtgataa acatatctgt aacttctttc agacctgctt
     3241 ctgcattcac tttcttcgca cggatagttt ccttgatttg gctgatcatt tctgggtcag
     3301 caagaaccat taagtgagca tcaaaaactt gagctgcttc ttcaccgagc gtacctactg
     3361 ctttctcgcg aataacagaa agctcgtctt gtgatgcctg tagagcggca tcaaggcgag
     3421 cttcttctgc gtttgtatct tcgactgtaa tagtctcaaa tgacaaatcc ggctgaacga
     3481 gtagatatgc ttttgcaact gcaacaccgt cagatgctgc gattccttta agcatttctg
     3541 tcatttccct tatgccaatc cttctttttc cattgtttct gagattgcag cgatagcgtc
     3601 atctgcatct gcaccttcag ctgagatagt tacgtcagca ccttggccaa caccaagact
     3661 cataacaccc ataattgatt taaggttaac tgatttacct ttgtactcaa gagtgatatc
     3721 tgaagcaaat ttgctagcag tttgtaccaa caatgttgct ggacgtgcgt gaatacctgt
     3781 ttctgccact acgtggaaat ctttagaagc catagtttga ctctcctttt gttctttctt
     3841 tttttgagtt atatgtgata acccttacaa tttagtatta taccatcttc taggattttt
     3901 ttcaagcatt ttctggattt tccttcaatt tcctttcaga taacttgctg aaaatcttct
     3961 atcctagata acaaaacaca ggatatagtt tcttcaaaaa caatttatag gataatcatt
     4021 gctatttcac aacacaaaca ctatatattg tgtttatata taaaaacaac aaaagaacac
     4081 cactatattt agttcaaatc attgacaaaa ttttattttc tgatatacta agatagtatt
     4141 aaattttgaa gaggagttac acaatggtaa ccgtttattc taaaaacaat tgtgtccaat
     4201 gtaaaatgac caagcgtttc ttggacagta ataatgtctc ttatcgtgaa atcaatcttg
     4261 atgagcaacc tgagtacgtc gatcaagtta aagagctcgg ttttagcgca gctcctgtta
     4321 tccaaacacc aactgaagtc ttttcaggtt tccaaccagg aaaactgaag caattagcat
     4381 aatcttagta catcatccag aaaaaactgc ttttagggct aacttagaag cctttctttt
     4441 gtaattagat aagggaaatt ttatgggatt aaaacatctt gaagacgtga cttacttccg
     4501 tcttaataac gaaattaacc gtcctgttaa tggacaaatc atgcttcata aagacaagga
     4561 agccttggat gctttcttta aagaaaatgt agttccaaac actatggttt ttgattcaat
     4621 caaagacaaa atcaattacc tcattgaaca caactacatc gaaacagcct ttatcaagaa
     4681 ataccgtcca gagttcttgg aagaattggc tcaatttatc aaagaccaaa acttccaatt
     4741 caagtcattc atggcagctt ataaatttta caatcaatat gccttaaaga ctaatgacgg
     4801 tgaatactat cttgaaaata tggaagaccg tgtcttcttc aacgcccttt atttcgctga
     4861 tgggaatgaa gctgttgcaa tcgatattgc caatgaaatc atccaccaac gctaccaacc
     4921 tgctactcct tccttcttga atgctggacg tgctcgccgt ggggagttgg tatcttgttt
     4981 cctaatccaa gtgacggatg atatgaactc tatcggacgt tctatcaact cagctcttca
     5041 actttcacgt attggtggtg gtgtgggaat taccctcagc aaccttcgtg aagctggtgc
     5101 acctatcaaa ggctatgaag gagctgcttc aggtgtcgtt cctgttatga agctttttga
     5161 agacagcttc tcttactcca accaattggg gcaacgtcaa ggtgccggtg ttgtctacct
     5221 caacgtcttt cacccagata tcatcgcttt cctttcaact aagaaagaaa acgctgatga
     5281 aaaagtacgt gtcaagactc tatcacttgg tgttgtagta cctgataaat tctacgaatt
     5341 ggctcgtaaa aatgaagaaa tgtacctctt cagcccttac tctgtagaaa aagaatacgg
     5401 tgtgccattc aattacatcg acattactga aaaatacgat gaattggtcg caaatccaaa
     5461 tattcgcaag acaaaaatca aggcgcggga tttggaaact gaaatctcta aattgcaaca
     5521 agagtctggc tatccttatg tagtcaacat cgatacggct aaccgtgcaa atcctgttga
     5581 tggaaagatt atcatgagta acttgtgttc tgagattctt caagttcaag aaccaagctt
     5641 gatcaacgat gctcaagaat tccttcaaat gggaacagat gtttcatgta acctgggttc
     5701 aaccaacgtg gttaacatga tgacttcacc tgattttggt cgttctatcc gtgctatggt
     5761 tcgtgccctt actttcgtta cagatagttc acatatcgta gctgtaccaa cgattgacca
     5821 tggaaatagc caagcccaca cctttggtct cggtgccatg ggactgcaca gctaccttgc
     5881 ccaacaactc attgaatatg gttcacctga gtctgttgaa tttacaagca tctactttat
     5941 gcttatgaac tactggacct tggtagagtc aaacaatatt gcgcgtgaac gtggtattac
     6001 cttccacaac tttgaaaaat cagactatgc taacggaagt tactttgaca agtatgtaac
     6061 tggtgaattt gttccaacat cagaccgtgt taaagaactc ttcaaaaatg tctttatccc
     6121 tggtgttgct gattgggctg aacttcgcga caaggttcaa gaagatggtc tttaccacca
     6181 aaatcgcctt gctgtagcgc caaatggttc tatcagctat atcaacgacg tttctgcttc
     6241 tatccacccg attacgcaac gtatcgaaga acgccaagag aagaaaattg gtaaaatcta
     6301 ctaccctgct gctggcttgt ctacagaaac cattccttac tacacttctg cctacgatat
     6361 ggatatgcgt aaagtcatcg atgtttacgc tgctgcgact gaacacgtgg atcaaggact
     6421 ttcactcacc ctcttcatgc gtagtgacat tccaaaaggc ctttacgaat ggaagaggga
     6481 aaataaacaa acgacacgtg atttgtccat ccttcgtaac tatgccttta acaagggaat
     6541 caagtctatc tactacgtcc gtacctttac agatgacggt ggagaagtcg gcgccaacca
     6601 atgtgaaagc tgtgtgattt agtttctagc atagaaacac tatttttccc taagcagcac
     6661 tgataaaatc aaatctacta tgtcataaca taatttatgt aacattttaa aagtcgggct
     6721 tccgactttt tgtgcgatac tcctatagaa ttatggtaaa gtagaaataa ttgtgagttc
     6781 gcaatactaa acacagaaag agattttaaa tggaaactta ctacaaagcc attaactgga
     6841 atgccatcga agatgtcatc gacaaatcaa cttgggaaaa actgacggag caattttggc
     6901 tcgatacacg tattccctta tcaaacgact tggatgactg gagaaagcta tctaacaaag
     6961 agaaagactt ggtcggaaaa gtttttggtg gtttaaccct tcttgacact atgcaatctg
     7021 aaactggggt tcaagccctt cgcgcagaca tccgtacacc acatgaggaa gctgttttca
     7081 ataacatcca atttatggaa tctgtccacg ctaaatctta ctcatcaatc ttttctacct
     7141 tgaatactaa ggctgagatt gaagaaattt tcgaatggac caataccaat ccttacctac
     7201 aaaagaaggc tgagattgtc aacgaaatct acctaaacgg cagcccactt gaaaagaaag
     7261 ttgccagcgt cttcctcgaa accttcctct tctactctgg tttcttcact cccctctact
     7321 atctcggtaa caacaaactt gccaacgttg cagaaatcat taaattgatt attcgtgacg
     7381 agtctgttca cggaacctac attggttaca aattccaact tggtttcaat gaattgcctg
     7441 aagaagagca agaaaaactc aaagaatgga tgtacgacct actctatact ctttatgaaa
     7501 atgaagaagg ctacacagag agtctttatg acggtgttgg ttggactgaa gaggttaaaa
     7561 ccttccttcg ctacaatgcc aataaagctc tcatgaacat gggacaagat ccacttttcc
     7621 cagattcagc tgaagatgtc aacccaatcg ttatgaacgg tatttcaaca ggaacttcca
     7681 accatgactt cttctctcaa gtcggaaatg gttaccttct tggcgaagtt gaagccatgc
     7741 aagacgatga ctataactac ggtttagact aattctccat cactctcaat catcaaaaag
     7801 taaactagaa acatcaaagt tataacccaa caaaaatatc gaaagttttg aaaacaaact
     7861 tcgatatttt tgtttttgtt tcattttgtt gtttttgtat ttgaatgatg agtttgttta
     7921 tggtaagata gtagtaggaa acgaggtgat aatatgctca aacaagaaaa attagccaaa
     7981 attttagaga tagtaaatag taaaggaacc ataacagtta aacagattat ggacgaaata
     8041 gccgtttcag atatgactgc aaggcgctat ttacaggaat tagctgataa agatttgctg
     8101 attcgtgtgc atggtggagc tgaaaaactt cgaaccaact cccttttgac taatgagcga
     8161 tcaaatattg aaaaacaagc cctccaaacg gcagaaaaac aagaaatagc ccattttgca
     8221 ggcagtctag tagaagaaag agaaactatt ttcattggac caggaacaac attagagttt
     8281 tttgcgcgtg agttgcctat tgacaatatc cgcgtcgtaa ccaacagtct acctgttttt
     8341 ctgattttaa gcgaacgaaa attaacagat ttgattttaa taggtggaaa ttatcgcgat
     8401 attacaggtg cttttgttgg tacattgacc ctacaaaatc tctctaatct ccaattttct
     8461 aaagctttcg ttagctgtaa tggtattcaa aacggagctc tagctacttt tagcgaggaa
     8521 gagggagag
//
LOCUS       DAVLRT010000078         8421 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLRT010000078 DAVLRT010000000
VERSION     DAVLRT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.078"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            44..397
                     /locus_tag="VXM85_001741"
     CDS             44..397
                     /locus_tag="VXM85_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1346283.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            612..728
                     /locus_tag="VXM85_001742"
                     /pseudo
     CDS             612..728
                     /locus_tag="VXM85_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            721..954
                     /locus_tag="VXM85_001743"
     CDS             721..954
                     /locus_tag="VXM85_001743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346284.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            1082..1224
                     /locus_tag="VXM85_001744"
                     /pseudo
     CDS             1082..1224
                     /locus_tag="VXM85_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1361..2329)
                     /locus_tag="VXM85_001745"
     CDS             complement(1361..2329)
                     /locus_tag="VXM85_001745"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1346285.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2474..3097)
                     /locus_tag="VXM85_001746"
     CDS             complement(2474..3097)
                     /locus_tag="VXM85_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV1346286.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSPFLNA
                     FESAIVAPLVEEPLKLLSLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(3079..3288)
                     /locus_tag="VXM85_001747"
     CDS             complement(3079..3288)
                     /locus_tag="VXM85_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346287.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKNGNCQRRF"
     gene            complement(3313..3810)
                     /locus_tag="VXM85_001748"
     CDS             complement(3313..3810)
                     /locus_tag="VXM85_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1346288.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(3883..5244)
                     /gene="radA"
                     /locus_tag="VXM85_001749"
     CDS             complement(3883..5244)
                     /gene="radA"
                     /locus_tag="VXM85_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1346289.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEVERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(5258..5773)
                     /locus_tag="VXM85_001750"
     CDS             complement(5258..5773)
                     /locus_tag="VXM85_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1346290.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(5775..6218)
                     /locus_tag="VXM85_001751"
     CDS             complement(5775..6218)
                     /locus_tag="VXM85_001751"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1346291.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(6275..6372)
                     /gene="ffs"
                     /locus_tag="VXM85_001752"
     ncRNA           complement(6275..6372)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VXM85_001752"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6405..6872)
                     /locus_tag="VXM85_001753"
     CDS             complement(6405..6872)
                     /locus_tag="VXM85_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1346292.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(7073..8359)
                     /locus_tag="VXM85_001754"
     CDS             complement(7073..8359)
                     /locus_tag="VXM85_001754"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1346293.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         2638 a         2041 c         1454 g         2288 t
ORIGIN      
        1 ttgttcacat cttatttcaa ttcactatag aaggaggaaa aggatgattc tcagacatct
       61 gggaattagc ccgaccaatg acttggttgc taagaaaatt ttcagcaacc cagaaatcac
      121 ttgtcaattt atccgcgata tgctggactt gccagccaaa aatgtgacta ttttggaggg
      181 aagtaacatt catgtcttgc cttccatgcc ctactcagcg caggattttt ataccagtat
      241 agacgtcttg gcggagttgg ataacggtac tcaagtaatt attgagattc aagtccatca
      301 tcagaatttt ttcatcaatc acttgtgggc ttacctgtgc agtcaggtta atcaaaatct
      361 tgaaaaaatt cgtcagcgag aaggtgatac tcactagatc tacaaacaca tcgctcctgt
      421 ttacgtcatt gctatcgtgg atagtaatta tttctcagat gacctggctt ttcatagctt
      481 tagtatgcgc gaagacacaa caggtgaggt attggcgatt accaacaatg gacaggaaaa
      541 ccatctggtt aagatggcat tcttggaatt aaaaaataca gagaaaccag caaagacaag
      601 gttcgcaagc catggttgga gtttttcggc aacaagccct ttacccagca accgcaacga
      661 gccattagcc aagcaaatca actgctggac tacaagagct ggtccgagga ggacaggaaa
      721 atgtttagtc aactacatat gcgagaagaa caagtcttgt tagcacagga ctatgccttg
      781 gaaactgcta gggctgaagg ccttgaacaa ggactagagc gtgggaaagt tgaaggaagg
      841 gcagaaagga aactttttgc cttcctagac atagtacgcc aaggtcttct gacttctgag
      901 gttgccagcc agcaattagg tatgtcagta tctgaatttg aggcactgtt gtaaaatggc
      961 tccataatat ccatagtggg taaatcccct atggatatta tggagcctat tttgtgtaaa
     1021 aaaaaaagtc ccatatgacc tataatgaaa agcgacaaaa caactcatta gaaagaatca
     1081 tatggaacaa ttacatttta tcacaaaatt actagacatt aaagacccta atgtccagat
     1141 tttaaacatc atcaataagg atacacacaa ggaaagcgtc gattcaaatg ctatcactgt
     1201 tcaaaaatga tggtcgctga aacttatgat gacgtacagt catatttctt ttctttttat
     1261 tatatcacag ttttaaatct agctttacta gattcaccgc tactatctat ttattcggaa
     1321 aaaagacgaa aaaacctgag aatcatctca ggcttggtca ttaaattttt ttctcaatat
     1381 cgaaaagtgg agaaagtggt cgtttttcat gaatacgtac gatagcatcc cctaggagat
     1441 gagcgattga aatctgctca atcttatcaa tcaaacgctc ttctggcaga tagatggtat
     1501 ccaaaacaac caatttctta atagctgatt tttggatatt gtccgtagca ggaccagaaa
     1561 gaactgggtg cgtacagctt gcatagactt caacagcacc agcttccgca agagcatctg
     1621 ccgcatgaca aatcgttcca gcggtatcaa tcatatcatc aatcaagata caagtcttgc
     1681 cttcaacctt accgatgatg ttcataactt cactagtatt catcttatca acgctacgac
     1741 gtttatcaat aatagcgata gatgttttca aaaattctgc caacttacga gcacgagtca
     1801 cccctccatg gtccgggctg acaaccacat agtcagaacc aaccatacca cgacgctcaa
     1861 aataatctgc aatcagagga gcacccatca aatgatccac aggaatatca aagaatcctt
     1921 gaatttgcgc agcatgcaag tcgatggtca ataaacgatc cactccagct acttcaagca
     1981 tatttgcgac aagttttgaa gtgattggct cacgcgctct cgcctttcta tcctgacgtg
     2041 catacccata gtaaggcatg acaacattga cagattctgc actcgcacgc ttcaaagcat
     2101 ctaccataat caaaatttca agcagattgt catttacagg cgaactagtt gattgtaaga
     2161 taaagacgtg tttcccacgg attgattctt caatgttgac ctgaatctct ccatctgaaa
     2221 attggcgaac acttgatttc cccaactcta tcccaatctc ctgcgccaca cgttctgcca
     2281 attctttatt agaagaaagg gcaaacagct ttaaatcaga aaaagacatg atttcctccg
     2341 gtatatatgt atagcttgtg cttttcacaa gattttccat ctaccattgt agcgcttttt
     2401 gcactatttt tcaatcaaaa ataaaagaag ggcaccatat ttgtaccctt gcatcattct
     2461 tttgaaaaat attctaggtc atcaactcat tgtgtttctc aacaaagcaa taagcatgat
     2521 aaaaaccata gagagcaata gccgtaacca ctggaatcgc taaaggcaac tctgtttcca
     2581 actccacaaa aggagagtta aacaagaagt gagttcccaa ggctaaacct agaaaaataa
     2641 ggccctgttt cttgccaacc ttctgtcctt tataggctct gtaaagcaag taaacaccta
     2701 ctacagctag acctgaaaaa gtccagtgag aggcaattcc tgagatgata cgctctaaaa
     2761 ttcgcgaaat agtaaagtca aagccctctg gcaaatccgt acgaatataa ccaatatcct
     2821 caatcatttg gaatcccaaa ccggaagcaa ttccaagtaa aaacaaagat tttaattttc
     2881 gcacaggaat caaagccaaa acaaaaacaa gtgacaataa tttcaagggt tcttctacca
     2941 aaggagccac aatagcactt tcaaaggcat ttaaaaatgg actatctggg aaaagaaccc
     3001 ccagtaaatc atggatataa gtattagcaa aactagacaa ccagcctgaa aggaacatcc
     3061 ctcccaataa agacagaatc aaaaccttct ttggcaattc ccatttttcc caatacggaa
     3121 gagaaaataa agagccggaa tcatgtaaaa gagagctaga aagatagaaa ctcccattag
     3181 tccatattcc gcacctgacc tcgaaccgtc cgtatagtag atggtttcat actgtaaacc
     3241 aatacatagc aataaaataa aaataaataa aatactgctt ttcttcatac actttctttc
     3301 taaatgaagt atttataatt ctacgactgt catacttcct gtatcaacat tgtaaatggc
     3361 accagagata atgacatcgt ctggtattag gggagactcg ataagcagtt gcatatcctc
     3421 gcgtacactc tcttctatat cttggaaggg caagaagtcc tggtctgaca catctacacc
     3481 taattcctct tttaaatact cctgaaaagg ttcattttca aaggtctgag caccacagtc
     3541 tgtatggtgc aataccacaa tctctcttgt ccccatttgt tgctgggaaa taactagcga
     3601 acgaatcatg tcttcagtca ctcgaccacc tgcattccgc aagatatgag catccccaag
     3661 tgccaagccc agagcttgcg caacgtgcag acgagagtcc atacaggtca caatagctac
     3721 tcttgttttg ggtttaagtg gcagatttaa ctgcccatgt agggcaacat aagcctgatt
     3781 ggcttgcata aactgttcaa aatacgacac gattccctcc ttgaaaattt gatagtcaaa
     3841 tatttctcct atcttatcat ttttaagaga atttgtcacg gattatgcaa agaccttttt
     3901 caagacttcc tgaatcgttg tcacgccaat gacctgaatt tccttaggca gagtgattcc
     3961 tgtcaaggaa ttcttaggta cataaatctt agtaaagccc agtttagcag cttcgttgat
     4021 gcgttgctca atacgattca cgcgccgaat ctctcctgtc aagcccagtt ctccgacaaa
     4081 acattcctga ggattagttg gcttgtcttt gtagctcgaa gcaatagcaa ctgcaacagc
     4141 caagtcaatc gcaggttcat ccaatttaac accaccagca gatttgagat aggcatcctg
     4201 attttgcaag agaagccctg cccgtttttc caaaacagcc ataatcaagc tagcacggtt
     4261 aaaatcaagt cctgtcgtag tacgcttggc atttccaaac atggtcggtg ttaccaaagc
     4321 ctgaacctcc gccaaaatcg gacgcgtccc ttccatggtt acaacgatgg aggaaccagt
     4381 cgccccatcc aaacgctctt ctaggaaaac ttgactcgga ttgagtacct caaccaagcc
     4441 gcccgactgc atctcaaaaa tcccaatctc attagtggaa ccaaaacgat ttttgaccgc
     4501 tctcaaaata cgaaaggtgt ggtgacgctc cacttcaaag taaagcaccg tatccaccat
     4561 atgctccaac atacgaggcc cagccaaggt tccttctttg gtcacatgac ctacgataaa
     4621 gatggcaatg ttattggtct tggccaactg catgagttca gcggtcactt cacgcacctg
     4681 agaaacagac ccctgcaccc ctgaaatctc aggagacatg atggtctgga tggaatcaat
     4741 aatgagaaag tctggctgga tacgctccac ttctgcacga acactctgca tattggtctc
     4801 tgcatagaga taaaactcac tatcaatatc acctaagcgc tctgcacgta gtttaatctg
     4861 ctgggcagac tcctccccac tgacatagag aactgtcccc acttgggaca actgggttga
     4921 gacttgtagg agaagagttg atttcccaat cccaggatcc ccaccgatga ggacgagact
     4981 tcctggtacc actccgcctc caagcacacg gttgaattcc tccatctccg tcttggttcg
     5041 attgacattg atggaagtca cctcagctag tttcatgggc ttggttttct cacctgtcaa
     5101 ggacacacgc gcattcttga cctcggcaac ctcaacctct tccacaaaag aagaccaaga
     5161 cccacagttg gggcaacgtc ccagatattt aggggaatta tacccacaat tttgacatac
     5221 aaatgtcgct tttttctttg cgatgacaaa cctctttcta tatctctaac tcacactcaa
     5281 tcacttggca aaaatcaatc ttctcatttg gcacaaactg gcgcatgagc attcgatgag
     5341 caacaactac cacagtctga tgttctcgat acttagacat acattctaga aaccgagact
     5401 tcatttccgt agctgtctca tattgaatag gactattagg aagcaactcc cccttgtttt
     5461 ctaaaaacag tcttctagct gtttcaaaat tttctattcc tgttttatag acctgccatt
     5521 catgtaataa aggctctact cttaaaggaa gacccgtagc acagaccaca tacgaagccg
     5581 tttctaaagc tcttgtgact gcagaagata cgattatttc agctgacgag agtaaaggat
     5641 ttttgctcaa tttctggact tgctgccgtc ccatctcaga caagggtgcc aaatctatcc
     5701 caaatcctat ataagaacgc tcctctaact cacggtaatc tggctcccca tgacgtacaa
     5761 agataatctt cattctagtg ccctgtcgat ccaaatccac cagttcgaac gccatcagct
     5821 gcatctccat ctgcaattaa gaaagtagca aaaacagcct ggacaatacg ttccccaact
     5881 tcaagaacaa cctcttgatc tgtgatattc ttcatctgcg caaaaatatg cccttcattt
     5941 ccaggatttc cataataatc cccatcaatg accccaactg agttaattaa aaccaagccc
     6001 ttcttacgag gatttgaaga acgatcatag aggtagagaa cctcagtcgg ctgcatataa
     6061 gccttaaccc ctgtcggaac caagacaatc tctcctggcg caacaactgt acgcacagca
     6121 acctttaagt cgtaaccagc cgcatgcgct gtctcacgct tgggcaataa attttcatct
     6181 gtaaaactcg aaaccaattc aaaaccacga attttcataa ttttctcttt tctattatca
     6241 tttattctag attattctat cttatttatt cggaaaaagc acgaaaaaaa gagcacacaa
     6301 ttcaaatcgc ttagggctgc tggattcctc ccctgacccg cttcacgcag aactgttgct
     6361 ccactattta ttatatcaca ttcctattca ttttaaaagc aaaattattt tttccgtcta
     6421 tttctaaaaa aatcctgcat gatagctgcg cattcatctt ccaaaattcc tatttcaacc
     6481 tccacacgat gattgagacg ctcatctgtc aagatattgt acaaactccc agcagcgcca
     6541 aatttctggt ttttagcccc atagaccaca tttggaatgc gggcaagccc aatcgctcca
     6601 ctacacatga cacaaggttc aatggtcaca aaaagtgtgc aatccagcaa gcgccagctc
     6661 tcctcactca agttcgcatc ctctatagcc ataatttccg catgcataac cgctcgctgt
     6721 aattcctcac gcgcattatg cccacgacca atgatttccc catctttgac aatcacacaa
     6781 ccaattggaa tttcatcgtg ttcaagagca atctcagcct ctctcaaagc ctccctcata
     6841 aagacttctt tttcttcaag cgtataatac atctcttttc tcttttccta cttatcgatt
     6901 ttattattat atcatgaatc ccaacacaaa aaaagccacc gaatgcggtg actctatagg
     6961 gagattatta tgaaaaagaa aagtttagga tatttgttac aacaagttag gaggtcttct
     7021 tgtaaccgtc tatagtatac ccgacctatc ttaaacaaat cttaaaaatc tcttaggacc
     7081 aaacactttc taaaatattt gtttgttcac gaccaggacc tactgagaaa gtagaaatac
     7141 gaacgccaac caattcactc acacgacgaa catagttacg cgcattctca ggaagatctt
     7201 ccaaattgcg aactccggta atatcttctg accaacctgg caactcttca tagataggct
     7261 tgcaacgttt caattgctca agactagctg gatagtagtc aatacgttga ccgtcaagat
     7321 cataggccac acagattttc acagtatcca aaccgctcaa aacatcaata gagttcaaag
     7381 aaaggttagt aataccagaa acacgacggc tatgacgcat cacaactgag tcaaaccaac
     7441 ctacacgacg tggacgacca gttgttgtac catactcatg acccacttca cggatacgtt
     7501 ctcccacttc atcaaacaac tcagttggga aaggaccatc tcctacacga ctcgtataag
     7561 ctttacatac acctacaacc ttgtcaatct tgcttggacc gacaccagaa ccaattgtca
     7621 caccaccagc tacagggttt gatgacgtaa caaatggata agtaccttgg tcgatatcta
     7681 gcataacacc ttgtgcacct tcaaaaagca cacgtttgcc attatcaagc gcatcattca
     7741 agataacaga tgtatctatc acgtatttct tgatttgttg accatattcg taatattctt
     7801 caaaaatatc atcgaaaaca atcgctttac tgtcatacaa tttttcaaaa agacgattct
     7861 tttcagcaag gttacgttct aaacgctcac ggaaaatatc tttatctaaa agatctgcaa
     7921 tacgaattcc aacacgagca gccttgtcca tataagctgg accaattccc ttaattgtcg
     7981 taccaatctt attgtcgccc ttagcttctt cttgcaagcg atccaactcg atatgataag
     8041 gcaaaataac atgcgcacga tcagaaatac gcaagttatc agttgttaca ccttcctcat
     8101 gaagatagct caactctttt acaagagatt taggatttac aaccatacca ttcccaatga
     8161 cagatatttt ttcagggaag aaaatcccag atggaatcaa gtgcaactta aatttcttac
     8221 cgtcaatcac aatcgtgtga ccagcattat caccaccttg gtaacgtgca atcacttctg
     8281 cattcgctga aaggaagtct gtaatcttcc ctttaccttc atcaccccat tgggtaccta
     8341 caacaacaac tgaagtcata atcttgtctg agccctcagg ctcttccttt ctcacataca
     8401 tggcaggact ctcacctgca a
//
LOCUS       DAVLRT010000079         8415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLRT010000079 DAVLRT010000000
VERSION     DAVLRT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.079"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            1..181
                     /locus_tag="VXM85_001755"
                     /pseudo
     CDS             1..181
                     /locus_tag="VXM85_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
     gene            196..1671
                     /gene="galT"
                     /locus_tag="VXM85_001756"
     CDS             196..1671
                     /gene="galT"
                     /locus_tag="VXM85_001756"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1346294.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1684..2694
                     /gene="galE"
                     /locus_tag="VXM85_001757"
     CDS             1684..2694
                     /gene="galE"
                     /locus_tag="VXM85_001757"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1346295.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            2874..3533
                     /locus_tag="VXM85_001758"
     CDS             2874..3533
                     /locus_tag="VXM85_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1346296.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3558..4625
                     /locus_tag="VXM85_001759"
     CDS             3558..4625
                     /locus_tag="VXM85_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1346297.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4729..5739
                     /locus_tag="VXM85_001760"
     CDS             4729..5739
                     /locus_tag="VXM85_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346298.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5751..7442
                     /locus_tag="VXM85_001761"
     CDS             5751..7442
                     /locus_tag="VXM85_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1346299.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7445..8155
                     /locus_tag="VXM85_001762"
     CDS             7445..8155
                     /locus_tag="VXM85_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1346300.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2850 a         1162 c         1649 g         2754 t
ORIGIN      
        1 acttcatgaa gttatcggac tgcatatgaa tagggctata tcttgttata gagaggaaca
       61 atctggatgt ttagatatgg ttgttataca aaaacagaat gagatagaag agcttagtac
      121 aaatattgaa aagaaaataa tgaattacat atttgaagat gatgggaatg tttctgaaat
      181 tagaaagaaa aatatatgaa aacaattatt gataattttg ttgatagagt aattgaatac
      241 ggaatgtata atgaaattga caaaatatat gtgaaaaata gagtactagc gttaataggt
      301 gaggagggaa ctgatcgcat ttccgatgaa aatgacctga aacaaataaa agattactta
      361 gtggaaatcg ccttaaaaaa cggtaaaatt aaagatttaa tcgaggaaaa ggaatgttta
      421 ggagcagagt taatgaattt catagttcct ttacctagtc gattaaatga tattttttgg
      481 agttcatatg atatatcacc tcaagaagca gttgaagagt tttataagtt aagtaaagat
      541 agtgattata ttaaaacttc tgctattgct aagaatattg agtttcgagc atcaactaaa
      601 tacggagagc tagagattac gataaatttg tcaaaacccg aaaaagatcc aaaaacaatt
      661 gctgctgaaa aattagtaaa agccactaat tatcccaaat gtttactatg tatggaaaat
      721 gaaggatatc aggggcgaat aaattatcca gcacgttcga atcatagaat tattcgattg
      781 aaacttggtg acgaagtatg gggattccaa tattctccct acttatactt taatgagcat
      841 gcgatatttt taaatagtca acatgtacca atggctatta cctctaaaac atttgaacaa
      901 ttattggaga ttgttgatat tttgccagga tattttgctg gttcaaattc tgatcttcct
      961 atctcaggag gctccattct tagtcataac cactaccagg gagggaaaca tattttccca
     1021 atggaaaaag caaaatttga gagtgaattt cgttttaaag actttgagga tgttaatgct
     1081 ggtattgtaa aatggccaat gtcagtaatt aggttacaaa gtgaaaataa aaatcgcttg
     1141 ttagatttag ctactaaaat tctaaataag tggagagaat attcagattt agaagtagac
     1201 atcattgcta tgactgaaga tgtcccacac catacggtga ctcctattgc tcgtaaagtg
     1261 gacgggagat atgaattaga tattgttcta agagataatc atactactga acagtaccca
     1321 gatggtgttt tccatcctca tcaagatgta caacatatta aaaaggaaaa tatcgggtta
     1381 atcgaagtga tgggacttgc gatattacca cctcgtttga aaccagagtt agaagaagtt
     1441 ggaaaatatc ttttaggaga ggataacgca attgcggatt atcatttaga atgggccgac
     1501 caattgaaag aaaaatatcc tcgcattaat aaagaagagg ttaatagcgt agttcaacac
     1561 gaagcaggac aagtgtttgc gagagttctg gaagatgcag gagtttataa gaatacaccg
     1621 tctgggcatg aagcgtttat gagatttgtt aagtcggttg gaattaatta ggaggaaaca
     1681 aaaatggcaa tattggtaac aggcggagct ggttatattg gtagccatac cgtagtagaa
     1741 ttactaaatt taggaaagga agtcatcatt gtcgacaacc tttcgaactc tagcatcttg
     1801 gtgctagacc gtattgaagc aattacagga atacgtcccg tgttttacga attagatgtt
     1861 tgtgataaac cagcattgag aaaggtattt gaacaagaat cgattgatgc tgcaattcat
     1921 tttgcaggtt ataaagctgt cggagaatcc gtgcaaaagc ctgtgatgta ctacaaaaat
     1981 aatattatga gtacattggc acttgttgaa gtgatgtcag aatttaatgt taaaaagatt
     2041 gtcttttctt caagtgcgac tgtatatgga attaacaatc agtcacctct aattgagact
     2101 atgcaaacaa gtgcgacaaa tccttatggg tatacgaaag tgatgcttga gcaaatttta
     2161 aaagatgttc atgtggcaga ttcagaatgg agtattgcgt tgcttcgtta tttcaatcca
     2221 attggtgctc atgagtctgg tttgattggg gaagatccct caggaattcc taacaacttg
     2281 atgccctata ttgcacaagt agcggtaggt aagctgtcag aactaagtgt gttcggaaat
     2341 gattatgata cgcttgacgg tactggtgtg cgcgattata tccatgtagt agatttagca
     2401 ataggacata taaaagcttt agaaaaagta tctgaaaaaa cagatgttta tatctataac
     2461 ctaggctcgg gagaaggcac tagtgtatta caacttgtaa atacatttga aagtgttaat
     2521 aagatcccta ttccttataa aatagttcca agacgttcag gagacgttgc aacttgttat
     2581 gcaaatgcag acaaagcata taaggaatta aattggagga caacaaaatc gattgaagac
     2641 atgtgtagag atacatggaa ttggcaatca aaaaatccca atggctataa ttgatgatac
     2701 tgtctatatg agtgctgtat tttggaaaaa ttacttaacc ttttaatgaa atgcatattt
     2761 aatgaaagat aaattaaaaa aaatgtttga cagcgcttga caaaggaaac ggtttcattg
     2821 tataatgtaa acacaatgac accgatgtca aatataaaaa agaaggtctt tatatgaaaa
     2881 acttaacaaa aattaagttc aaagaaaatg gggaatttaa tcatttccct gggaatacag
     2941 ttgtagcaaa tctttatact aaacaagatt tgatggaagt tgttgatatt attcaatcac
     3001 gttatagaga attaccattt atcgataagt ttactttaac tccaaggaat tctattcaca
     3061 tgactgtaat tgaattgttg tgccatgaaa atcgtgaaac ggagttttgg agcagtaatc
     3121 ttcctctaga tacaccttta caggaaatac atgattactt tgctaaacaa cttgaaattt
     3181 ttccattgtt ggatgaagaa attcatatgc gtataactga aatgggaaaa caaaacatac
     3241 tagttgaacc tgcagatgaa gcttctgcaa agagattaga agaaattcgt acttatgtct
     3301 cagaaaaagc aggtgtttgt ttccctaatc atgatagata tcaattccat atttcaattg
     3361 ggtatcttcg gattcctcta accgaagagg aggaagaaga gtttactaaa gtcagagcag
     3421 aattaactga aattttatta gagaagattc caactattac tgtaaaccgt attgactata
     3481 ctgtatttga agatatgagg caatttgttc catatcacga aaaatttaaa taaaataata
     3541 aaaaaggaga acttatcatg aaaaaaatga aagtttggtc tactgtactt gcaacgggag
     3601 ttgctcttac tacacttgct gcttgctctg gaggttcaaa ttctacgact gcttcttcat
     3661 ctgaagaaaa agctgataaa agtcaagaat tagttatcta ttcgaactca gtctcaaatg
     3721 gtcgtggtga ttggttaact gctaaagcaa aagaagctgg ttttaatata aaaatggttg
     3781 atatcgctgg cgctcaatta gcagaccgtg ttattgctga gaagaataat gcagttgcag
     3841 atatggtatt tggaattggt gctgttgact caaataaaat tagagatcaa aaattactag
     3901 tacagtacaa gcctaaatgg ttagataaaa ttgatcaatc tttatcagat aaagataatt
     3961 attataatcc tgtgattgtt caaccattag ttttaattgg ggcgcctgat gtaaaagaaa
     4021 tgcctaaaga ttggactgaa ttaggtagta agtataaagg taaatattca atttatggtc
     4081 ttcaaggtgg tacaggacgg gcaattctag caagtatctt agttcgttac cttgatgaaa
     4141 aaggagattt aggcgtatca gaaaaaggat gggaagttgc aaaagaatac tttgcaaatg
     4201 cctatactct tcaaaaaggt gaaagctcag ttgttaaagc gttagataaa gaaagcccaa
     4261 ttcaatacgg tatgatctgg ggttctggag cgttaattgg acaaaaagaa caaaatgttg
     4321 aattcaaagt tatgactcct gagattggtg taccatttgt aactgaacaa actatggttt
     4381 taagcactag taaaaaacaa gcgttagcta aagaatttat tgattggttt ggtcaatcag
     4441 aaattcaagt agaatatagt aaaaaatttg gatctattcc tgccaataaa gatgccctaa
     4501 aagagttacc tgaagatacg aaaaaatttg ttgatcaagt gaaaccacaa aatattgact
     4561 gggaagctgt tggaaagcat ttggatgaat gggtagaaaa agctgaatta gaatacgtaa
     4621 aataagaaat aagagttatt ttttatgtcc aagtctctct attatttcta aatttaagga
     4681 agaaataaag agaggctttt ggttttagta atagagggag ttagtgagat gattaaattt
     4741 gataatattc aaattaaata tggtgatttt gttgcaattg ataatctgaa tttagatata
     4801 catgaagggg aattctttac atttcttggg ccttcaggat gtggtaaatc aactactttg
     4861 agagcattgg taggtttttt agatccatca tcaggaagta ttgaagttaa tggaacagat
     4921 gtcactcatt tggaacctga aaagcgtgga attggtattg tatttcaatc ttatgcgcta
     4981 tttccaacta tgactgtttt tgataatatt gcatttggtt taaaagttaa gaaggtagct
     5041 ccagatgtta ttaaagctaa agtatcagca gtggcagcaa aaattaagat ctctgatcaa
     5101 cagttacagc gtaatgtatc agaattatct gggggtcaac aacaaagggt agcattggct
     5161 cgtgctctgg ttcttgaacc taaaattctt tgtctagatg aaccattgtc aaaccttgac
     5221 gcaaaattac gtgtagattt gagaaaagag ttgaaaagac ttcaaaaaga gttaggtatt
     5281 actactttat atgttactca tgatcaagag gaagccttga ctttatctga tagaattgca
     5341 gtctttaaca atggatacat cgaacaggtc ggtacaccag tagagattta tcataattct
     5401 caaactgaat ttgtatgtga ttttattgga gatattaatg ttttgaccga tgaaacagtc
     5461 cacgaagtat tactgaaaaa tacaagcgtt ttcttagagg ataaaaaagg ttacattcga
     5521 ttagagaaag ttcgattcaa tcgtgaaact gaacaagatt ttattctaaa agggacaatt
     5581 attgatgttg agttttctgg agttacaatt cactatacaa taaaagtttc tgaaagtcag
     5641 attcttaatg taacaagtat tgatagtcag gctgctatta gatctgtcgg agaaagtgtg
     5701 gaattattta tcacaccatc agacgttctg caattttaag gaggtgcagt atgcgtcata
     5761 aattaaattt aaaagattgg cttattcgtt tagggttaat ctggttctta gtaacattta
     5821 ttatttatcc aaactttgat ctagtagtga atgtatttgt aaaaggagga gaattttccc
     5881 ttgatgctgt acatcgtgtt ctaaaatctc agagggcact tcagagtatt atgaacagtt
     5941 ttaagttagc attttcactc attattacag ttaatgtcgt aggtattctt tgtgttctat
     6001 ttacagagta ctttgatatt aaaggtgcta aaattttaaa attaggttat atgacctctt
     6061 taatttatgg aggagtggtt ttagcgactg gatataaatt tgtctatggt ccttatggat
     6121 tgattacaaa atttttacaa aatgttatcc cttctttaga ctctaactgg tttattgggt
     6181 atggtgcagt cttattcatt atgacatttt caggaactgc taatcataca ttgtttttaa
     6241 caaatacaat tcgaagcgtt gactatcaca ctattgaggc tgctcgaaat atgggagcaa
     6301 aaccatttac tgttttccga aaagtagtgt taccaacctt aattccaact ctatttgcac
     6361 ttactattat ggtttttctt agtggtttat ctgcagtagc agcacccatg attgttggtg
     6421 gtaaagaatt tcaaactata aatccaatga ttattacatt tgcagggatg gggaattctc
     6481 gtgatttagc tgccttactt gcaattattt taggtattgc aactacaatt ttgcttacta
     6541 tcatgaataa gatagaaaaa ggtggaaatt atatttctat ctctaagact aaagcgcctc
     6601 ttaaaaaaca aaaaattgcg tctaagcctt ggaatatcat tgctcacatt gtagcatatg
     6661 gattgttcac agttttcatg cttccactaa tttttatagt attatactca tttacagatc
     6721 cagttgcaat tcaaacaggt aacttaacat tatcaaactt tactttagaa aattatcgct
     6781 tattctttag taatagtgcg gcattctctc cattcttggt cagctttatt tattctatta
     6841 ttgctgcgac aacagcaaca attctcgcag ttgtatttgc tcgtgttgtc agaaaacata
     6901 aatctcgttt tgatttctta tttgaatatg gtgctctact tccttggtta ctaccaagta
     6961 cacttttagc agtaagttta ttatttactt ttaatcagcc acaatttctt gtcttgaatc
     7021 agattttggt aggtagtttg gtaattctac ttattgcata tatagttgta aaaatcccat
     7081 tttcttatag aatggtacgt gctattttat ttagtgttga tgatgagatg gaagatgcag
     7141 caagaagtat gggtgcttca cctttttata ctatgatgaa ggttatcatt ccatttattt
     7201 taccggttgt tctctctgtt attgctttaa actttaactc tttattaact gacttcgact
     7261 tatctgtatt cctttaccac cccctagctc aaccattagg tattacgatt cgatctgcag
     7321 gtgatgaaac agcaacatct aatgcacaag ctctggtatt tgtttataca attgttctga
     7381 tgattatttc tggaacggta ttatacttca cacaaagacc ggggcgtaaa gtaaggaaat
     7441 aatcatgaca gccactagtc ttgggttatc aaatattgaa atagttgtca ggattgtttt
     7501 atcagtagtc attggtagta taattggttt agagagaggg agcaaatccc agcctgcagg
     7561 catccgaact tatagtattg tttgtctagc tgcatgtttg attatgatga cgaatgaata
     7621 cgtatcttat aaatttggga caggagatcc tacacgatta ggagctcaag ttatatcagg
     7681 tgtgggtttt ctaggcgctg gaacgattct tattacagat aaaaagaaaa ttacaggtct
     7741 gacaactgca gcaggcattt gggcttcggc aggaattgga ttagctattg gagtaggttt
     7801 ttatgaggga gctcttttag tagccatttc tgtttggggt gtgatatcca tgttccaacc
     7861 actaaaaaaa tatctacaaa atcgttctaa aatgattgaa ttgtatatag tagttaaatc
     7921 tacagaagcc tataatcgtg tattggttta ttgtgcagaa aatggtatca gaatgaccga
     7981 ttcaagaaca gcgtttggag atgttaattc tgacagaatt gaatactttg atgttccaga
     8041 taaaaaaata gcgtcattta ttactctgga attatcaggt agatttgaac atcttcgact
     8101 gatggaagaa attgctaata ttgtaggtgt tatttatgtc gaagaaatta gctgaaattt
     8161 agtaaacaaa gggacagatg tttttgtcct tttgtttttt agatatttta atcatctaat
     8221 ttttgagtag atttttgctc agaggttttt atgaaagatt ataaaatcaa ttttgattta
     8281 ggtaaaatag aatattttga taataattgt ttgatacaag tatacaaatt tatttctttt
     8341 tatgatattt gtgaaatggt gtttgccttt catttacctc ctgatgagtt gataacaaat
     8401 gttatcttta aagaa
//
LOCUS       DAVLRT010000080         7932 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLRT010000080 DAVLRT010000000
VERSION     DAVLRT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.080"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            120..2753
                     /gene="polA"
                     /locus_tag="VXM85_001763"
     CDS             120..2753
                     /gene="polA"
                     /locus_tag="VXM85_001763"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1346301.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNMSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDQLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEIKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2838..3275
                     /locus_tag="VXM85_001764"
     CDS             2838..3275
                     /locus_tag="VXM85_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1346302.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(3460..4470)
                     /locus_tag="VXM85_001765"
     CDS             complement(3460..4470)
                     /locus_tag="VXM85_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1346303.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVINAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            4619..5788
                     /locus_tag="VXM85_001766"
     CDS             4619..5788
                     /locus_tag="VXM85_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1346304.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLKDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            5785..6555
                     /gene="recO"
                     /locus_tag="VXM85_001767"
     CDS             5785..6555
                     /gene="recO"
                     /locus_tag="VXM85_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1346305.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            6552..7544
                     /gene="plsX"
                     /locus_tag="VXM85_001768"
     CDS             6552..7544
                     /gene="plsX"
                     /locus_tag="VXM85_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1346306.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIVGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            7550..7783
                     /locus_tag="VXM85_001769"
     CDS             7550..7783
                     /locus_tag="VXM85_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1346307.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            7829..7932
                     /locus_tag="VXM85_001770"
                     /pseudo
     CDS             7829..7932
                     /locus_tag="VXM85_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2248 a         1463 c         2026 g         2195 t
ORIGIN      
        1 tttaaatcta ggtttactaa attctctggc tctatctatt tattcggaaa aagtttgaaa
       61 aatacttgct ctagctcttc ccaatggtat tttttgattc tttcctttat aatgggtgta
      121 tggataagaa aaaattatta ttgattgatg ggtcttctgt agcttttcgg gcgttttttg
      181 cgctgtatca gcagttggac cgttttaaga atgcggctgg tttgcatacc aatgcgattt
      241 atggttttca gttgatgttg agtcatttgt tggagcgggt tgagccgagt catattttgg
      301 tggcttttga tgcgggaaag acgaccttcc ggacagagat gtatgcggac tataagggtg
      361 gtcgggccaa gactcctgat gagtttcgtg agcaatttcc tttcattcgt gagttgctgg
      421 atcatatggg gattcgtcac tatgagctgg ctcagtatga ggcggatgac atcattggga
      481 cgctggataa gctagcagag caggatggtt ttgatattac cattgtcagt ggggacaagg
      541 atttgattca gctgacggat gagcatacgg tggttgaaat ttccaagaaa ggtgtggctg
      601 agtttgaggc ctttacgcca gattacctca tggaagaaat gggcctcaca ccagctcagt
      661 ttatcgatct caaggcgctc atgggtgata agtcggataa tatccctggg gtgaccaaag
      721 tcggtgaaaa gacgggtatt aagctcttgc tggagcatgg ttcgcttgag gggatttatg
      781 aaaatattga tggaatgaag acttctaaga tgaaggaaaa tctcatcaat gacaaggaac
      841 aggccttttt gtctaaaaca ctagcgacca ttgataccaa ggcaccgatt gcgattggtt
      901 tagaggactt ggtctatagt ggtccagatg ttgaaaatct tgggaaattc tacgatgaga
      961 tgggcttcaa acagctcaag caggctttaa atatgtcgtc agctgatgtg gctgagggtt
     1021 tggattttac tattgttgac caaatcagtc aagatatgct gagtgaagag tctatcttcc
     1081 actttgagct ttttggtgag aattaccata cagataattt ggttggattt gcctggtctt
     1141 gtggggatca gctctatgcc acagacaagc ttgagctgtt gcaagacccg attttcaagg
     1201 atttcttaga aaaaacatct ctgagagttt atgactttaa gaaggttaaa gttcttttgc
     1261 aacgttttgg tgtagatttg caggcgcctg cttttgacat ccgtttggct aaatacctcc
     1321 tttcgactgt ggaggacaat gaaattgcga ccatcgctag tctttatggt cagacttact
     1381 tggttgatga tgaaactttc tacggtaagg gtgttaaaaa ggccattcct gaacgtgaga
     1441 aattcttgga acacttagct tgtaaacttg ctgttttggt agaaacagag cctattttac
     1501 ttgaaaaact tagcgaaaat gggcaattag agcttcttta tgatatggag caacctctgg
     1561 cttttgtcct tgccaagatg gaaattgctg ggattgtggt caagaaagag accttgcttg
     1621 agatgcaggc tgaaaatgag cttgtcattg aaaaactgac tcaagagatt tacgagctgg
     1681 ctggtgagga gtttaatgtc aactcgccta agcagttggg cgtgcttctc tttgagaaat
     1741 tgggacttcc tctagaatac actaagaaaa ccaagacagg ttattcgaca gcagtggatg
     1801 tgttggagcg tctcgctcct attgctccga ttgttaagaa aatcctggat taccgtcaaa
     1861 ttgctaagat tcaatctact tatgtaattg gcttgcagga ctggattttg gctgatggaa
     1921 agattcatac tcgctatatg caggatttga cccagaccgg gcgtttgtct agtgtggatc
     1981 caaacttgca aaatattcct gcccgattgg aacaagggcg cttgattcgg aaggcttttg
     2041 tgccagagtg ggaggatagt gtgctactca gctctgacta ttcacagatt gaattgcgcg
     2101 ttttggcgca tatttctaag gatgagcact tgattaaggc cttccaagag ggggcagata
     2161 tccatacttc gacagccatg cgggtctttg gcattgagcg tcctgataat gtgactgcaa
     2221 atgaccgtcg caatgccaag gcagttaact ttggagtggt ttatgggatt tcagactttg
     2281 gcttgtctaa taatttggga attagtcgta aggaagccaa agcctacatt gatacctact
     2341 ttgaacgttt tccaggtatt aaaaactaca tggatgaagt ggtgcgggag gcgcgtgata
     2401 agggctatgt agagaccctc tttaagcgtc gccgtgagtt gccagatatc aattcgcgca
     2461 acttcaatat tcgtggtttt gcggagcgaa ctgctatcaa ctcacctatc cagggttcgg
     2521 cagcagatat tctcaagatt gccatgattc agctggataa agccttggtt gcaggtggtt
     2581 atcagactaa gatgctgtta caagtgcacg atgaaatcgt ccttgaagtg cctaaatctg
     2641 aattggtaga gataaaaaaa ttggtgaaac aaaccatgga agaagccatt caactcagtg
     2701 ttcctcttat cgcagatgag aatgaagggg caacctggta cgaggctaaa taaaaagggg
     2761 gctagtcctc cttttttgta gtagaattct gcaaaccttt tcaaaatgtg ctatactgat
     2821 gaaaaaggag gatttctatg agtcaagaat ttatcaatcc aagtgatggc gtgattcgtc
     2881 agtatctcgc aacgagtaaa acccttgctg tggtgggttt gtctgaccgt gaagaaacaa
     2941 ccagcaatcg agtaacaaag gaaatgcagg ctcggggcta taaaatcatc ccagtcaatc
     3001 ccaaggcggc aggtggcgaa atcttgggtg aaaaggctta tgctagcctc gctgaaattc
     3061 cttttcctgt agatattgtc aatgtttatc gtcgcagtga atttctgccc gatgtggcgc
     3121 gtgattttct caaagctgat gccaagattt tttgggcaca gctaggactt gaaagtttag
     3181 aagcaaaaga gattttgcgt gatggtggat gtgatgatat cgtgatgaat cgctgtatca
     3241 agagagaaca tactcgcttg attgaggaag cataagaaaa aggtagctga tgggctacct
     3301 tttgtgttat actcaatgaa aatcaaagag caaactagga aactagctgc gggttgctca
     3361 aaacaccgtt ttgaggttgc agatagaact gacgaagtca gtaacatata cctacggcaa
     3421 ggcgacgttg acgtggtttg aagagatttt cgaagagtgt tagaaaatgc cgataagggt
     3481 ctgcatacca agagtggtga ggatgatggc aatccagcag atggctccaa gaagaatgga
     3541 ttttccactg gatttgacca tagcgaccag attggttttg agaccgatag cactcatggc
     3601 catgacaata aggaatttag agagttcttt gagaggggta aagaaactac tggacacacc
     3661 tagagaggtg agtagtgtag tgaggagaga ggcaaggata aagtaaagga taaaaagtgg
     3721 gaagactttt ttcagttgta ggctttgctt gttttcttgt tggcgacttt gccagtagga
     3781 tagaaagagc gtgataggga taatggccaa agtacgtgtg agtttaacaa tggttgcaga
     3841 ctcgagggta ttgctttggt aaagactgtc ccaagcgctg gcggcagccg ttacagagga
     3901 agtgtcgttg accgcagtcc ctgcaaagag ggcgaagcct tcattggata gatgaagcca
     3961 ggtgccgagg gttggaaaga tgagcgcagc caagacattg aagaaaaaga taacggaaat
     4021 ggcttgggct acttcctttt ccttagcatt aataacgggc gctgtcgctg caatggcaga
     4081 acccccacag atagaagaac ctactccaac caaggtagcc agttttgtat ccagtgcaaa
     4141 gaaacgctgg aagaggtagg caataatcag agctattgag atagtggaca ggatgacagg
     4201 gagtgaagat tggccaactg cgaagacctg cgagatattg agaccaaaac caagcaagac
     4261 aacggcatat tggagcaact tcttggaact aaaggtcaaa ccagcatcca gttgtttata
     4321 ggacgagaga aagggatgta ggagcatgcc tatgaaaatg gcaaaaacgg gcgcgccaat
     4381 cacagggaag aatcctccta agtaccaaga tacgatagaa atgagaaggc aggccaagat
     4441 acctgctcca ttttttgata gaaatgacat aaaaacctcc aaataaaatc tattaccatt
     4501 atagacctat aaacaggaaa agtaaaatag aaagtggaaa gctattctct aatgtatttt
     4561 tgcggtcggg aggcttttgt agtataatag agatacgttt tgaaagtagg aggtatctat
     4621 ggacttaact aagcgcttta ataaacagtt agataagatt caagtttcgt tgattcgtca
     4681 gtttgaccag gctatttcgg agattcctgg ggtcttgcgt ttgaccttgg gggaacctga
     4741 ttttacaacg ccagaccatg tcaaggaggc ggccaagcga gcgattgatc agaaccaatc
     4801 ctactataca gggatgagtg gtctgctgac tctacgtcag gcagctagtg actttgttaa
     4861 ggaaaagtac caactggact atgctcctga aaatgaaatc ttggttacaa ttggggcgac
     4921 agaggcttta tctgcgactt tgacggctat tttggaagag ggagacaagg tacttttgcc
     4981 agctcctgct tatccaggct atgaaccgat tgttaactta gttggggcag aaattgttga
     5041 gattgatacg actgaaaatg gttttgtctt gactcctgag atgttggaga aggccatttt
     5101 ggagcagggt gataagctca aagcggttat tctcaactat ccagccaatc cgacaggaat
     5161 tacctacagt cgagagcagt tagaggcctt ggcagctgtt ttacgcaagt acgaaatttt
     5221 tgttgtctgt gatgaggttt actcagaatt gacctacaca ggcgaagccc atgtgtctct
     5281 aggaacgatg ttgaaagacc aggctattat tatcaatggt ttgtctaaat cgcatgccat
     5341 gacaggttgg cgtttggggc tgattttcgc tcctgcggcc ttcacagccc agttaatcaa
     5401 gagtcaccaa tacttggtca ctgccgcaaa taccatggcg caacatgctg cggtagaagc
     5461 cttgacggct ggtaaaaatg atgcggagcc catgaagaag gaatatatcc agcgtcggga
     5521 ctatatcatc gaaaaaatga ctgctcttgg ttttgagatt atcaaaccag acggtgcctt
     5581 ctatattttt gctaaaattc cagcgggcta caatcaagac tcctttgctt ttctgaagga
     5641 ttttgctcag aagaaggccg ttgcctttat ccctggtgca gcctttggac gttacgggga
     5701 aggctacgtc cgcctatctt atgcagccag catggagact atcaaagaag ccatgaaacg
     5761 acttgaggag tacatgagag aagcatgatt cagtctatca cgagtcaagg cttggtgctt
     5821 tacaatcgca attttcgtga ggatgacaag ctcgtcaaaa tttttacaga gcaggttggc
     5881 aaacgcatgt tttttgtcaa acacgctggt cagtctaagc tggcgcctgt tattcagccc
     5941 ttggtgttgg cacgatttct cttgcgaatc aatgatgacg gactcagtta catcgaagac
     6001 tatcatgagg tcatgacttt tcccaagatt aatagtgacc tctttgtcat ggcctatgcg
     6061 acctatgtgg cagctcttgc agatgctagt ttgcaggaca atcagcagga tgctcccttg
     6121 tttgcttttt tgcaaaagac tttggagttg atggaagcag gcttggatta tcaggttttg
     6181 accaatattt ttgaaattca aattttgact cgatttggaa tcagcctcaa ttttaatgag
     6241 tgtgtcttct gccatcgggt tggtcaggct tttgactttt ctttcaaata tggagcctgc
     6301 ctctgtccag agcattatca tgaggataag agacgttgtc atctcaatcc caatatcccc
     6361 tatctgctca atcaatttca agctattgat tttgagactt tggagaccat ttcgctcaag
     6421 cctggaatca agcaagagct acgccaattt atggatcaat tatatgaaga gtacgttggg
     6481 attcacctaa aatcaaagaa atttattgat tccctagcag actggggaca attactaaaa
     6541 gaggaaaaga aatgaaaaaa atcgcagtag atgccatggg gggcgattac gcacctcagg
     6601 ccattgttga gggtgtcaat caagccctat ctgacttttc agatatcgag gttcaacttt
     6661 acggagatga agctaaaatc aagcaatatc tgacagcgac agagcgcgtc agcattatcc
     6721 atacggatga gaagattgat tcggatgatg aacctacgag agctattagg aataagaaaa
     6781 atgccagtat ggtattggca gccaaggctg tcaaagatgg tgaagcagac gctgtccttt
     6841 cggctgggaa tacaggtgcc ttgttggcag caggattctt catcgtgggt cgtatcaaga
     6901 atatcgaccg tcctggactc atgtctacct tgcctaccgt tgatggaaaa ggttttgaca
     6961 tgctagacct tggtgccaat gcagaaaata cagcccagca cctccatcaa tatgcggttc
     7021 taggttcctt ctatgctaaa aatgtccgtg gcattgcgca accacgcgtt ggtttgctca
     7081 acaacggaac agagagtagc aagggtgacc cgcttcgtaa ggaaacttat gaattactgg
     7141 cggctgatga aagtttgaac tttatcggaa acgtggaagc gcgtgatttg atgaatggcg
     7201 ttgcagatgt tgttgtggca gatggtttca cgggaaacgc tgtgctcaaa tccatcgaag
     7261 ggacagctat gggaatcgtg ggcttgctca agacagctat tacaggtggt ggtcttcgag
     7321 cgaaactagg tgccctcctt ctcaaggaca gcctcagtgg tttgaaaaaa cagctcaatt
     7381 attcagatgt tggtggagcg gtcttgtttg gtgttaaggc acctgttgtc aagactcatg
     7441 gctcaagcga tgccaaggct gtttatagta cgattcgtca gattcgtacc atgctagaaa
     7501 cagacgtggt tgcccagact gcgcgtgaat tttcaggaga ataaaagaga tgacagaaaa
     7561 agaaattttt gaccgtattg tgaccattat ccaagagcga cagggagagg actttgtcgt
     7621 gacagaatcc ttgagtctga aagacgattt ggatgcggat tctgttgact tgatggagtt
     7681 tatcttgact ctggaagatg aatttagtat cgaaatcagc gatgaggaaa ttgaccaact
     7741 ccaaagtgta ggagatgtgg ttaaaatcat tcaaggaaaa tagcaatcgg agttccaagt
     7801 caacggaagt agatggtttt tagaaatgag aaatattgga caagctggta aaatcttggc
     7861 tgacagtggt tatcaagggc tcatgaagat atatcctcaa gcacaaactc cacgtaaatc
     7921 cagcaaactc aa
//
LOCUS       DAVLRT010000081         7649 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLRT010000081 DAVLRT010000000
VERSION     DAVLRT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.081"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(115..390)
                     /locus_tag="VXM85_001771"
     CDS             complement(115..390)
                     /locus_tag="VXM85_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1346308.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(496..1335)
                     /locus_tag="VXM85_001772"
     CDS             complement(496..1335)
                     /locus_tag="VXM85_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1346309.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1477..2325
                     /locus_tag="VXM85_001773"
     CDS             1477..2325
                     /locus_tag="VXM85_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1346310.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFIFGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2418..3335)
                     /locus_tag="VXM85_001774"
     CDS             complement(2418..3335)
                     /locus_tag="VXM85_001774"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1346311.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3770..3975
                     /locus_tag="VXM85_001775"
                     /pseudo
     CDS             3770..3975
                     /locus_tag="VXM85_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            4309..5615
                     /locus_tag="VXM85_001776"
                     /pseudo
     CDS             4309..5615
                     /locus_tag="VXM85_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5755..6048)
                     /gene="rpmA"
                     /locus_tag="VXM85_001777"
     CDS             complement(5755..6048)
                     /gene="rpmA"
                     /locus_tag="VXM85_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1346312.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(6065..6409)
                     /locus_tag="VXM85_001778"
     CDS             complement(6065..6409)
                     /locus_tag="VXM85_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1346313.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(6425..6739)
                     /gene="rplU"
                     /locus_tag="VXM85_001779"
     CDS             complement(6425..6739)
                     /gene="rplU"
                     /locus_tag="VXM85_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1346314.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQVVYVEKLNVEADQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(6775..6862)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(6957..7556)
                     /locus_tag="VXM85_001780"
     CDS             complement(6957..7556)
                     /locus_tag="VXM85_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1346315.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
BASE COUNT         2385 a         1565 c         1263 g         2436 t
ORIGIN      
        1 tacaatattt tcggaacatt caacttttta actctattta ttactagatt tcataattaa
       61 aaagcctact gaccaagcta gaaagcttga tacaataggc tttttaaaga ctgattattt
      121 aacagcgtct ttaagagctt taccagcttt gaatgctggt actttagaag ctgcaattgt
      181 catttcttta ccagtttgtg ggttgcgacc tttacgttct gcacgctcac gaacttcaaa
      241 gttaccaaaa ccgatcaatt gaactttttc accagctgca agatagtcag ctactgctgc
      301 aaatacagct tcaactgctg ctgctgagtc tttcttagtc aattctgtag cttctgctac
      361 tttagcgatc aaatcttgtt tgtttgccat gttaatgatt cctccagata atttctaatt
      421 aacaaatata atcatatcct aaaatccctt ttaggtcaag ttaaaaacgc tatttcttcc
      481 aattttattt attttttagg agtggtaacg tatcaaaata gcccaagcgt tctcacccgt
      541 gtgagtttga ataatggaac ccgtttccaa aacagaaatt ggcttttcaa cataagcttg
      601 taagctttct ttcatctctt ttgcccaatc atcactacca gaatatgaaa ttccaatctc
      661 tgctacagca cgttcagaaa gcgatgttat caactcatct aaccattttt taaatgtttt
      721 agttccacga cctttaacca ttggctgcaa ttcatggtct ttcatttgca tgacaacacg
      781 gatattgaga agggagctca acaagccagt tacacggcca attcgtccac ctttgacaag
      841 attttccaaa gttgaaacac caatataaag ctctgtatgg tttttaacct cttctacatg
      901 agataaaatt gcctccatat ctttaccttc ttgagctaac ttcgcagcct caacaacttg
      961 gaatttcagg gcttggtcag tgaaggaact atcaacaaca gtcacatctg cagtagatag
     1021 gctagcacct tggcgtgctg cttctaccgt acccgaaaga gcatgggaca tatgaatagc
     1081 aagaatctgg ccaccatctt tgcataggtc ttcaaaaatc tcagcaaaga cacctacagg
     1141 tggctgactt gttttcggaa gattcttact ttcttgcatc aactgaagaa atttaccttc
     1201 ttctttcaaa tccgcatcag aataaacaac attatcaatc attacagata atggaacaat
     1261 tgtaatatct aattgcttta ctagttcagg ttcaatagta acagatgaat cggttacaat
     1321 cttaattttt gtcatagtat caatctttct attttaggat tcagattggt ttccttactt
     1381 ctaattatat caaaaaaaag attaaaaatc ctaatggagt caatcaaatt ttccgtaaaa
     1441 tttgatataa tcaacttata agaaaagagg tgtcctatga ttaaaaaaat ttaccccatt
     1501 tttaccattt tactaggtgc tgctatttat gcttttggac tgacttattt tgtagttccc
     1561 catcatctct ttgaaggagg ggcgacaggc attaccctca tcacctttta tctttttaaa
     1621 atccctgttt ccctcatgaa cctgctgatt aatattcccc ttttcatcct agcttggaag
     1681 atctttggag ccaaatccct ctattctagt ttactaggaa ccttagcttt gtccgcctgg
     1741 ttagcttttt ttgagcatat tccccttcat attgatcttc aaggtgattt actaatcaca
     1801 gcccttatag cgggaatcct attgggaatt ggccttggaa ttatttttaa tgctggaggt
     1861 acaactggcg gaactgatat tctagctcgt attctcaaca aatacactca tatatccata
     1921 ggaaaactgc tctttatctt agatttttgt attctcatgt tgattctcct aatcttcaag
     1981 gatttgagat tggtttccta cacgcttttg tttgatttta ttgtttctcg tgttattgat
     2041 ttgattggtg aaggaggata tgccggcaaa ggctttatga ttatcacaaa acgtcctgac
     2101 caacttgcta aggcgattaa tgatgacctc ggaagaggtg ttacttttat ttttggtcaa
     2161 ggctactata gtaaagaaaa tttgaaaatc atctactgta ttgtcggaag aaatgaaatt
     2221 gtgaaaacga aggaaatgat tcatcgaatc gatcctcaag cctttataac tattacagaa
     2281 gcccatgaaa tcctaggaga aggcttcacc tttgaaaaag aataaaaaga ggtaatgtcg
     2341 tgacctcaaa agttagacta aatcatctat cttttgggtt acagacaacc tcttttttgt
     2401 tttatttact caagctctta agaccaattc cgagttactt cttcatcagc ctttaactga
     2461 tccactaatt ggtcaactga gtcaaatttg gtcatatcac gaatgcgatc aagccaataa
     2521 accatgacgg tttccccata aatatcttga ttaaaatcaa aaatattgac ttcaaaacgt
     2581 gcttcttctc catcaaaggt cacatttttc ccgacactag ccatagcacg atacttctgt
     2641 ctttgaatct caacatcaac gacataaacg ccatctgctg gcatataagt acggtctaaa
     2701 agcactaaat tcgctgtcgg ataaccaatt gtacgaccac gagcattacc atgaactacc
     2761 atacctcttg atggaagcgg tgcccccaaa agttttcctg cttctttcac atttccatct
     2821 aaaatagctt gacggatacg agttgaacta atctttcctt tctcatcttc tacaggtgga
     2881 acaatgataa cttctccatc aaagtaatcc tttaaatctt ctgctgtttt tttgtcagaa
     2941 ccaaatgtat aatcaaaacc tgcaacaata attttggcat tcatagcctt gatataagtt
     3001 gcaaagaatt cttgtgcagt gagactagcg aattgactac taaaatcaag gagatataat
     3061 tcttctacac cttcgcgctt tagttttctt tcacgttcag cagggttcaa aatatgcaaa
     3121 aacaaatctg gatgataagg ctctaaagcg atctttggag attcattaaa ggtcataacg
     3181 acgataggta ataaatcctt tctcgcagcc ttgttggcaa cacgaaataa ttcttgatgc
     3241 cccttatgta tgccatcaaa atagccgaga acaacgactg aatcagatgg tgtgccaata
     3301 tctttttggt tttttatagg aatagtaata atcataaaat aattatatca tagcgatagc
     3361 tatttctgga acagaaaatc tgaaatgttg tttttttcac atgaagtgta cctgttttca
     3421 aaaagcactt tattctatcg ttgcttaact atgaactttg caatattctt ctcaaaaact
     3481 tgtaggacat cttcaaaatt ttgcaaggag tgattagact tgttcggtaa ccataaagtg
     3541 tcatactatg cttatgtatg aaaaagcaat gcaactaact cctgagaact ttaaattact
     3601 aattggtgcc gaaaaggtag aatttagaat cgaggtacac ctatggctgt aaaatttaca
     3661 aaatgagaca acttgggcaa gatgtttgaa gaatttccta aactccctga tttgaagcaa
     3721 gtcactttcc ctgatgacaa aaaaagccaa agcagtaaag aaaaactaga tgactgcttt
     3781 ccaacaactt ccatctagtg tgcttcagac tgggctattt ttctctccat ctgttagctt
     3841 ggattctcag accgtttcag ctaaagaata tcttttccct tatcagaagg aacggctcaa
     3901 gccattcaga caagtgaagg gacgacaagc caatatttga aaccagatag cagttcttat
     3961 agtcaattga aataaaatct gaagaaatcg agtaggaaac tcaaatcaat ttttaacagt
     4021 gttctattcc agattcatac tcaatgaaaa ttaaagtgca aactaggaag ttagccgcag
     4081 gctgtacttg agtacggcaa ggtgaaactg acgtggtttg aatttgattt tcgaagagta
     4141 tcaatccact atacatatta aaaagtatgg gtatcaaaat aaaaaggctc tataatattt
     4201 gtagtgggta aatccactat agatattatg gagcctattt tgtgtagaaa aaaagtccca
     4261 tatgacctat aatgaaaagc gatcaaacaa ctcattagaa agattcatat ggaacaatta
     4321 cattttatca caaaattact agacattaaa gaccctaata tccagatttt agacatcatc
     4381 aataaggata cacacaagga aatcatcgcc aaactggact acgacgcccc atcttgccct
     4441 gagtgcggaa atcaattgaa gaaatatgac tttcaaaaac cttctaaaat tccttatctt
     4501 gaaacgactg gtatgcctac tagaattctc cttagaaagc gtcgtttcaa gtgctatcac
     4561 tgttcaagaa tgatggtcgc tgaaacttct atcgtcaaga agaatcacca aattcctcgt
     4621 attatcaacc aaaaaattgc gcaaaagttg attgagaaga tttctatgac cgacattgat
     4681 catcagttgt ctatttcaac ttcaactgtc attcgcaaga tcaatgattt tcactttaaa
     4741 catgattttt cttgtcttcc tgagattatg tcttgggatg agtatgcctt cactaaggga
     4801 aagatgagtt tcattgcgca agattttgat aatcttaata ttatcactgt tcttgaaggc
     4861 agaacacaag ctgtcatccg aaatcacttt cttcgctacg atagagccgt tcgttgtcaa
     4921 gtgaaaatca ttacgatgga tatgtttagt ccttactatg acttggctaa acagcttcgc
     4981 tttcaaattt ctaggctcag gctgaaacag tctcccagac tgtttcactc ccgaatgcta
     5041 aaatcgttct tgatcgcttt cacattgtgc aacatcttag ccgtgctatg agtcgtgtgc
     5101 gtgttcaaat catgaatcag tttcatcgaa aatcccatga atacaaggct atcaagcgct
     5161 actggaaact cattcaacag gatagccgta aactgagtga taaacgtttt tatcgtccta
     5221 cttttcgcat acacttaaca aataaagaaa ttcttgacaa gattttaagc tattcagaag
     5281 acttgaaaca ccactatcag atctatcaac tcttactttt tcactttcag aacaaagacc
     5341 ctgagaaatt tttcggactc attgaggaca atctgaagca ggttcatcct atttttcaga
     5401 ctgtctttaa aacctttcta aagaacaaag aaaaaatcgt caacgctctt caattacctt
     5461 attccaacgc aaaattggaa gcgaccaata atctcatcaa acttatcaaa cgaaacgcct
     5521 ttggatttcg gaactttgaa aacttcaaaa aaaggatttt tatcgctctg aacatcaaaa
     5581 aagaaaggac gaattttgtc ctttctcgag cttagctgac ttcaacccac tacagttgac
     5641 aaagagccta aaaaaacagg ctctccgaaa actcggaaag ccttatttta agctgtttct
     5701 agcttcctca cctttatatt tcaaggttcg ggataaaagg ttcaatggac ctttttattt
     5761 agcgattggg taaacagaaa cttgtttttt atcgcgtcct ttacgttcaa agcgtactac
     5821 gccttcaact ttagcgaaca aagtatcgtc tccaccacga ccaacgttta cacctggata
     5881 gatgtgtgta ccacgttgac ggtaaaggat tgatccacct gttacagttt gtccgtcagc
     5941 tgctttagct ccaagacgtt tcgcttgtga atcacgtccg tttgatgtag aacctccacc
     6001 ttttttgtgg gcgaaaagtt gcaagttgtt aagagtcatt tttaacataa tgttttcctc
     6061 cgtgttagtt ttctgtgata actctggttt ggacgaactc agaagagttc tccgataagt
     6121 ttgccatacc taagaaaaat gattcaaaga ataactgggt catttctctc tggtgtgaag
     6181 gaagatcttt tggtatttca accatcagat agccaccttc atcttcgttt aattctagga
     6241 ttggttcata gcctgcaaat ttctcaatag aattgataaa gttaatggca agcgtagaaa
     6301 ccgatgcaca cacgacatct aagccgtatt cgccactctc ggcgtgtcca gtaatttccg
     6361 cactcctcag ctcgccatct tcggctctct caaagactgc ctgtatcatg tgttctcctt
     6421 aaaattaagc gttgattgcg ttgatgacaa cttttgtata tggttgacgg tgaccttgtt
     6481 tacggtggct acctttttta ggtttgtact tgtaagtaac cactttcttt tgttttcctt
     6541 gtttttcaac agttccaact acagtagctc cagcaacaag tggagttccg acaacagtgt
     6601 tttcaccacc aacaagaaca acttcgttaa aagtaacttc ttgatcagct tcaacgttca
     6661 atttttcaac gtaaactact tgaccaactt caactttaac ttgttttccg ccagttttga
     6721 taattgcgta tgtgctcatt atgcacctcc tatgattttt atgggtttcc ccgatttttt
     6781 gtgaagactc gcctagcatc gtgggacgaa ccacttaaac ttgaataatc aagcaacgat
     6841 gttcgtgcgg ttgcacagga tcgtgcatag tcaactcttc aagtatagca tatctcctat
     6901 tttcttacaa gtaataacac ctaaaatgaa gctttttctt ttactttttt ctgccactag
     6961 gcaaaaagca tactgagata aaaaatgctc atcataatag gaacaccaag aatggtcttt
     7021 tcatgataga aaatcgtcaa ataggctgaa aagacatcgc caaggacaaa actactaagc
     7081 aggctaacaa atatgaaccc ttcacgcaaa aaaggagtgt gcttagttcg gaaataatct
     7141 ccaaaagcta gcattgttcg tttgatattc cctgtcataa aggcattatt gtaggcaata
     7201 cccgaaacct caccaaaagc agttgtcacc agtcccatac agaaggccaa gggctgcact
     7261 agatagatat tatctacagt ttgtggcaca aaaccaataa ttattgatag gattgctaaa
     7321 ggaatcaagg acagaatagg ttttttcaca attctcaatt tttccttata aatcgttaat
     7381 aaaaagactc ccattataaa cgttattaag gttaaaagct tatccctaac atccgaaaca
     7441 ttattttgta ttagctctac cgaaagaaag acaacatttc cagcttgtcc agctacaagg
     7501 gtattccctc gaacaataaa agtgtaagca tccacatatc cagcacaaaa tgtcaaaaaa
     7561 gtgctaacct tttagactga cgtgatattt ttcttatagg taataacctc attttccctc
     7621 ccattgtatt ttctcttaga aatattgta
//
LOCUS       DAVLRT010000082         7639 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLRT010000082 DAVLRT010000000
VERSION     DAVLRT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.082"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            14..556
                     /locus_tag="VXM85_001781"
     CDS             14..556
                     /locus_tag="VXM85_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1346316.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(597..670)
                     /locus_tag="VXM85_001782"
     tRNA            complement(597..670)
                     /locus_tag="VXM85_001782"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Asn,seq:gtt)
     gene            complement(676..747)
                     /locus_tag="VXM85_001783"
     tRNA            complement(676..747)
                     /locus_tag="VXM85_001783"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Glu,seq:ttc)
     gene            complement(799..1551)
                     /locus_tag="VXM85_001784"
     CDS             complement(799..1551)
                     /locus_tag="VXM85_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1346317.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1548..2873)
                     /locus_tag="VXM85_001785"
     CDS             complement(1548..2873)
                     /locus_tag="VXM85_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1346318.1"
                     /translation="MDLFGFGTVIVHFLIISHNYHLICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIATFLFPLGLYSYFRWIKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFS
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2894..3019)
                     /gene="comC"
                     /locus_tag="VXM85_001786"
     CDS             complement(2894..3019)
                     /gene="comC"
                     /locus_tag="VXM85_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1346319.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(3185..3258)
                     /locus_tag="VXM85_001787"
     tRNA            complement(3185..3258)
                     /locus_tag="VXM85_001787"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3222..3224),aa:Arg,seq:tct)
     gene            complement(3302..3781)
                     /gene="rlmH"
                     /locus_tag="VXM85_001788"
     CDS             complement(3302..3781)
                     /gene="rlmH"
                     /locus_tag="VXM85_001788"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1346320.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEELSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3964..5145
                     /locus_tag="VXM85_001789"
     CDS             3964..5145
                     /locus_tag="VXM85_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV1346321.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            5203..5961
                     /locus_tag="VXM85_001790"
     CDS             5203..5961
                     /locus_tag="VXM85_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1346322.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            6174..7535
                     /gene="dnaA"
                     /locus_tag="VXM85_001791"
     CDS             6174..7535
                     /gene="dnaA"
                     /locus_tag="VXM85_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1346323.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2653 a         1380 c         1211 g         2395 t
ORIGIN      
        1 acaaggagga aatatgcaag aaagtaacaa acgcttaaaa acaaagcgaa ctattgaaaa
       61 tgctatggta caattactga tggaacagcc atttgataaa atttctactg tcaaattagt
      121 agaaaaagcc ggaattagtc gttccagctt ctatactcac tataaggata agtatgatat
      181 gattgagcac tatcaaagca agctattcca tacatttgaa tatatttttc aaaaacatgc
      241 tcatcacaaa agagacgcta ttttagaagt ttttgaatat ctagagtcag aaccacttct
      301 ggctgccctt ctttctgaaa atgggactaa agaaatccaa aatttcttac gaaataaact
      361 tcatatcatg cttagtacag atttacaaaa gcgatttatg caactgaatc taaataccac
      421 tgaattagaa tatagtagca tctatctaac tcatgcactt tttggtgtct gccaaacttg
      481 gattgcacat ggaaaaaaag aaagttctca agaaataaca gacttcctta tgaaaatgct
      541 tggtgataca aattgataca aaaagaggaa cacagctgtg ttccctttta tctatactcc
      601 gccagtagga ctcgaaccta cgacatcatg attaacagtc atgcgctact accaactgag
      661 ctatggcgga taaaatagtc cgtacgggat tcgaacccgt gttaccgccg tgaaaaggcg
      721 gtgtcttaac cccttgacca acggaccttc tatctgtagc agatataacc attatatcaa
      781 tttcttgcta attgtcaatc acttttgaga ttttttctct aaaatatctt ttaattttct
      841 aatttttaat cttgaaatag gacaacgatg gtcttcatag aaaacaattt ctaagttttt
      901 tcgatcaatt tctctgatat tgcctatatt taccaaaaat gacttgtgag gagaataaaa
      961 tcgctgagta tgtttgtcct tttcctgaat atctgtcatg gtaccataaa actcttttgc
     1021 aaaattctta ccaataatgc gcaatttatg agatacccct gttgtttcaa tatacaaaat
     1081 atcatggtaa ggaattttta aatcatttcc cttgtaattg tagtcgaaat aatctacaac
     1141 atcttcattt tcaagtaaca tactcttcgt gtagaagata ttttgctcaa ttctcttctt
     1201 aaacatctca tcattgatat ccttatcaac aaaatctagg gctgatacct ggtatttata
     1261 ggttagagtc gcaaactctg atcgactagt gataaagacg ataatagcgt aaggattgta
     1321 atgacgaatg agctgagcca cttcaaatcc ctttttctca attccatgaa tatcgatatc
     1381 taggaaataa agctgattta cttcatcatt ttcaatgtat tcttcaaatt cacggacttt
     1441 tcccgttgtc ttgtatgata ttggaatatt cgattctttc gaaatttcat ccaatattct
     1501 ctctagtctc acttgatgtt caataacatc ttctaaaatt aaaactttca ttcaaattcc
     1561 ctcttaaatc taatgatttg tctaaatgta ctgccttcca tctctgtttc taaaataata
     1621 ttgttgtact tatctagtag ttctttcaca ttatttaatc cgactccgcg atttcttccc
     1681 ttagtggaga atcctaagga aaatagatct cctgaaggag tcatcgtcat tttacatgaa
     1741 ttctgaatca caataactgt ttcagtttcc atcttaataa ctgctacttc catctgcttt
     1801 ttatagctat cagccgatcc ttcgacagca ttattcaata aaacgctcat gatacgaacc
     1861 aaatccaata gttcaattgg aagcttggta atcgtatctt ttacttccag tgtaaactct
     1921 acaccattat ttcgagcata gacaattgac tgagcaacca aacttcgtaa agctgagtct
     1981 tctatgttgt tcaaatcaaa gtaagtgtac ttatctgaac gcaatttatg atttgctttg
     2041 actaaaactt cattgtaaat tctgtcaatt tcctgtaaat taccactgtc aattgccatc
     2101 tgcatgctga caagcattcc agcataatca tgtcgaaaac cacggatttc attatacaga
     2161 ccaacaattt catctgtgta attctgtaaa tgtttctgtt caaatttctt ctgcttcaaa
     2221 gcaatctctt tctccatttg aactttatga gaattcattg caaagaaggt caaaaggaga
     2281 gagataaaga caatagatga caaaatactt ccaaaactat tcaaatgttt aatcgtactt
     2341 accatatctg aaacgaaaga tacaatatgt agcaatagta aagcaaaaaa tacttttttc
     2401 aagaaaggat aaaggtagtc cttgtcaaaa taggctagtt ccaaatggaa atagtaaatg
     2461 atttttaatg taacaaaata ggttaacacc gtcacaacga aaaagaatgg gaaatgatat
     2521 tgtaaaacaa aattatctcc tgttatagag gagaaagtta cggacagaaa gttatgagtg
     2581 ctctcatata aaagagatag tagtaaactt aggaatagtc ctctatccct ctcatactgt
     2641 tttatccatc gaaaatagga atataggccc aaaggaaata aaaatgttgc aatccctaac
     2701 ccatctaaat atagaatata taagggaaaa tcaaatacta tttctgttag taatgtataa
     2761 gcaccaaaaa cgaataattc ttttctattt atttgacctt tacaaattaa atggtagttg
     2821 tgactaataa ttaaaaaatg aacaataact gtcccaaatc caaataaatc cattactctt
     2881 tccccttatt tcattacttt tttctttgta aaataaaatc acggaagaat tttgacagcc
     2941 tcatctcccc acctttaatc ttttgtaagt ctttttcctt caaagctaca aactgttcca
     3001 atttaactgt gtttttcata ataaaatctc ctaaaatgtt tttttcttgt aagctaactt
     3061 acaaaaacca ttatacaaaa tggaatttcg ttttagataa aattctctca actgtcattt
     3121 ttttctccca aagtgtactt ttttaagaaa aaagccggga aaattcccag ctttgctatt
     3181 atattgatcc cagcaggatt cgaacctgcg accgttcgct tagaaggcga atgctctatc
     3241 cagctgagct atgagaccta acacaattat tctaccaaaa attcaattaa aagtcaattt
     3301 tctatttatg gtaggggaat ccctgctgaa tcgtaaaagc gcgatagatt tgttcaacaa
     3361 gaactagtct cattaactga tgaggcaagg ttaggcgacc aaaactgaca gaaagattgg
     3421 ctctattttt tacagatgat gacaatccta aacttccccc aataataaaa gtaagagtag
     3481 aaaatccttt tatagaagtt tcttctaact gcttactcaa ttcttctgag aagaaagttt
     3541 tcccttcaat ggctaacaca ataacgaaat cacggtcagc aatttttgat aaaattctct
     3601 gaccttctat ttctaaaatc ttttgatttt ctgattcact ggccttatct ggtgtttttt
     3661 catctgataa ctcaatcatt tcaaacttag caaatctaga aattcgtttt gaatactctg
     3721 cgataccatc ttttaaatac ttttctttca gtttcccaac tgttacaact ttaattttca
     3781 tgactctatt ctaacatatt ctctattttt tcacatctta ttcacaaaat aaaaaataga
     3841 tttcaattaa gaaaatcaca gtttcaaaag agttatccac agtttgtgta aaacttttgt
     3901 gtttaagtta taattaagct agtcagttta tactttcagt aattcaaaca tatggaggca
     3961 aatatgaaac atctaaaaac attttacaaa aaatggtttc aattattagt cgttatcgtc
     4021 attagctttt ttagtggagc cttgggtagt ttttcaataa ctcaactaac tcaaaaaagt
     4081 agtgtaaaca actctaacaa caatagtact attacacaaa ctgcctataa gaacgaaaat
     4141 tcaacaacac aggctgttaa caaagtaaaa gatgctgttg tttctgttat tacttattcg
     4201 gcaaacagac aaaatagcgt atttggcaat gatgatactg acacagattc tcagcgaatc
     4261 tctagtgaag gatctggagt tatttataaa aagaatgata aagaagctta catcgtcacc
     4321 aacaatcacg ttataaatgg cgccagcaaa gtagatattc gattgtcaga tgggactaaa
     4381 gtacctggag aaattgtcgg agctgacact ttctctgata ttgctgtcgt caaaatctct
     4441 tcagaaaaag tgacaacagt agctgagttt ggtgattcta gtaagttaac tgtaggagaa
     4501 actgctattg ccatcggtag cccgttaggt tctgaatatg caaatactgt cactcaaggt
     4561 atcgtatcca gtctcaatag aaatgtatcc ttaaaatcgg aagatggaca agctatttct
     4621 acaaaagcca tccaaactga tactgctatt aacccaggta actctggcgg cccactgatc
     4681 aatattcaag ggcaggttat cggaattacc tcaagtaaaa ttgctacaaa tggaggaaca
     4741 tctgtagaag gtcttggttt cgcaattcct gcaaatgatg ctatcaatat tattgaacag
     4801 ttagaaaaaa acggaaaagt gacgcgtcca gctttgggaa tccagatggt taatttatct
     4861 aatgtgagta caagcgacat cagaagactc aatattccaa gtaatgttac atctggtgta
     4921 attgttcgtt cggtacaaag taatatgcct gccaatggtc accttgaaaa atacgatgta
     4981 attacaaaag tagatgacaa agagattgct tcatcaacag acttacaaag tgctctttac
     5041 aaccattcta tcggagacac cattaagata acctactatc gtaacgggaa agaagaaact
     5101 acctctatca aacttaacaa gagttcaggt gatttagaat cttaattgac atctatgtaa
     5161 agaaagcttt acataagaga aaagatgtgt tagtgtagaa tcatggaaaa atttgaaatg
     5221 atttctatca cagatataca aaaaaatccc tatcaacccc gaaaagaatt tgatagagaa
     5281 aaactagatg aactagcaca gtctatcaaa gaaaatgggg tcattcaacc gattattgtt
     5341 cgtcaatctc ctgttattgg ttatgaaatc cttgcaggag agagacgcta tcgggcttca
     5401 cttttagctg gtctacggtc tatcccagct gttgttaaac agatttcaga ccaagagatg
     5461 atggtccagt ccattattga aaatttacag agagaaaatt taaacccaat agaagaagca
     5521 cgcgcctatg aatctctcgt agagaaagga ttcacccatg ctgaaattgc agataagatg
     5581 ggcaagtctc gtccatatat cagcaactcc attcgtttac tttccttgcc agaacagatt
     5641 ctctcagaag tagaaaatgg caaactatca caagcccatg cgcgttccct agttgggtta
     5701 aataaggaac aacaagacta tttctttcaa cggattatag aagaagatat ttctgtaagg
     5761 aagttagaag ctcttctgac agagaaaaaa caaaagaaac agcaaaaaac taattatttc
     5821 atacaaaatg aagaaaaaca gttaagaaaa ctactcggat tagatgtaga aattaaacta
     5881 tctaaaaaag acagtggaaa aatcattatt tctttttcaa atcaagaaga atatagtaga
     5941 attatcaaca gcctgaaata aggctgttct tttatttttt tatctcacaa ggttatccac
     6001 tatgtttttc gataaaaagc ttaataaatc aataatttct tcttttatcc ccaacctgtg
     6061 gataaagttt agtaacattg tggattattt ttcacagctt gtggaaaatt cttgctatct
     6121 atggtaaaat atctctagta ttaaactttt aaatagtaaa ggaggagaaa ggattgaaag
     6181 aaaaacaatt ttggaatcgt atattagaat ttgcacaaga aagactgact cgatccatgt
     6241 atgatttcta tgctattcaa gctgaactca tcaaggtaga ggaaaatgtt gccactatat
     6301 ttctacctcg ctctgaaatg gaaatggtct gggaaaaaca actaaaagat attattgtag
     6361 tagctggttt tgaaatttat gacgctgaaa taactcccca ctatattttc accaaacctc
     6421 aagatacgac tagctcacaa gttgaagaag ctacaaattt aactctttat gactatagtc
     6481 caaagttagt atctattcct tattcagata cgggattaaa agaaaagtat acctttgata
     6541 actttattca aggggatgga aatgtttggg ctgtatcagc cgctttagct gtctctgaag
     6601 atttggctct gacctataac cctcttttta tctatggagg accaggcctt ggtaagactc
     6661 acttattaaa cgctattgga aatgaaattc taaaaaatat tcctaatgcg cgtgttaaat
     6721 atatccctgc cgaaagcttt attaatgact ttcttgatca cctaagactt ggggaaatgg
     6781 aaaagtttaa aaagacctat cgtagtcttg atcttttgtt aatcgatgat atccagtcac
     6841 tcagcggaaa aaaagtcgca actcaggaag aatttttcaa tacctttaac gcccttcatg
     6901 acaagcaaaa acagattgtc ctaacgagtg atcgtagtcc aaaacatcta gaagggctcg
     6961 aggagaggct tgtcacgcgt tttagttggg gattgacaca aactatcaca ccccctgact
     7021 ttgaaacacg tattgccatt ttacaaagta aaacggaaca tttaggctac aatttccaaa
     7081 gtgatactct agaataccta gctgggcaat ttgattcaaa tgttcgagat cttgagggag
     7141 ccatcaacga catcacttta attgccagag taaaaaaaat caaggatatc actattgata
     7201 ttgctgcaga agccattaga gcccgcaaac aagatgttag ccaaatgctc gtcatcccaa
     7261 ttgataaaat ccaaactgaa gttggtaact tttatggtgt tagtatcaaa gaaatgaagg
     7321 gaagtagacg ccttcaaaat attgttttgg cccgtcaagt agccatgtat ttatctagag
     7381 aactaacaga taatagtctt ccaaaaattg ggaaggaatt tgggggaaaa gatcatacca
     7441 cagtcattca tgcccatgcc aaaataaaat ctttgattga tcaagacgat aatttacgtt
     7501 tagaaattga atcaatcaaa aagaaaatca aataatttgt ggataacttt tagtttttta
     7561 tcttttttat ccacattttt taaacaagct aaaaaacttg atatgacttg tttaaaggct
     7621 gttttccaca gatttcaca
//
LOCUS       DAVLRT010000083         7630 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLRT010000083 DAVLRT010000000
VERSION     DAVLRT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.083"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            143..442
                     /locus_tag="VXM85_001792"
                     /pseudo
     CDS             143..442
                     /locus_tag="VXM85_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            513..2534
                     /locus_tag="VXM85_001793"
     CDS             513..2534
                     /locus_tag="VXM85_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1346324.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2536..3177
                     /locus_tag="VXM85_001794"
     CDS             2536..3177
                     /locus_tag="VXM85_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346325.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3281..4633
                     /locus_tag="VXM85_001795"
     CDS             3281..4633
                     /locus_tag="VXM85_001795"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1346326.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATD
                     KNTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4637..5695
                     /locus_tag="VXM85_001796"
     CDS             4637..5695
                     /locus_tag="VXM85_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1346327.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5705..6904
                     /gene="divIB"
                     /locus_tag="VXM85_001797"
     CDS             5705..6904
                     /gene="divIB"
                     /locus_tag="VXM85_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1346328.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     NQRSSRR"
BASE COUNT         2369 a         1312 c         1662 g         2287 t
ORIGIN      
        1 aaaaattcga ttttttcctt gacaacaaat aataaaaaag ttattcttat cacataagaa
       61 agcggtttca ataaaagaga gaggaggatt tattttgttg cattgttgct caaggtgtgc
      121 tttttttgaa agaaaaaata aaatgaaaac gaaaaaacat agattacttg ctctagctct
      181 tatttcaaat tttacattat tgggagctgc atcagctgct gtacaatatc cagatggagg
      241 agtatggaca tatggagaag gttcaggagg tggttaggct ttttcaaatt actatcatgg
      301 taaaaaatat cattattcat ccatcgtgag cagatgggat ggtcattctg ataaaggaga
      361 agctcctgct ggaaaaacct cctatgcatg gatttggact aaatggggag aacaagtagc
      421 attttactgt gattatgact aatcgttcat tgatttaaat cgttcccctt gtaagaaatt
      481 acagggggaa ttttgtttac aaggagtcta ctatgaagaa actattcata ttattatcaa
      541 ctttttttct cagcttcttc cttgcttgga ttattgtctt acgtgcgcca caatatttat
      601 atgcaagcta tgattccgtt tctttacttc gtgtcaaaaa agatactcag gaaccgacgc
      661 gtgaggtatt tgaacaggaa ttggagaatt ttgcaaactc agaacagagt ttaatagcta
      721 gaagaattgt agagccgagt aaggatggga cgactcactt tacttatgca acttatggtc
      781 agggaacttt accaaaagaa ttccaagaag ctagccaaga aagtcgtgaa cgtagtgatc
      841 cgctaaatag ttatctcctt ttgtcaggct ccttgacgaa agaaaagctt gccgataaat
      901 taggagattt gggttataaa gcaagtgctg accgaaagat accgccctat tttcttgctt
      961 ttcgaatatt actaaatccc cttattttaa ttagtttagc aatatttggc ttatctttct
     1021 ttgctttagt gattatcact cggattaagg aaatgagagc agcaggtata aaactctttt
     1081 ctggtcagac tctcttatcc atcatggggc attctttatc tactgatatc aaatggctcc
     1141 ttctatcagc cctcctttcc ttcctaggtg ggggagtcgt tctttttagt caaggtttgt
     1201 tttatcctat cttgttagcc acctatggtt ttgggattag tttctatctg ttgtttttat
     1261 tggcgatttc aattttacta atgcttcttt atctaatgag tttgaattac aaagcattag
     1321 ttcccgttat taaggggaga ttacccctta aacgcctgat gattttaacc ctattgtgtc
     1381 agttggttgc tgtctttaca gtaggatacg ctgttaagac aggtttgacg tcttaccaac
     1441 gattgaaaga acttgaaatt tcaaaacaag catggcagga tagagcagac tattatcaaa
     1501 tttcttttgg cttaggtgat agaggaaaag atacagaaaa tcagagcaag tggtatgcct
     1561 ttgccaagga agcaatcgaa gaagaacaag ctctttatgt aaaggataat ctgctccatt
     1621 ttgccaatcc acaaggaaaa aatgaacagg gagagacact ggatacctat agtccagatg
     1681 ctaatacgct ctatgttagt cccagttatt tggacaagga aaaggtcgtg gtagatgctg
     1741 agaccaaaca gaagttagcc catctccaaa aaggtgagtt tatcctcttg ctcccagaac
     1801 atttgcgctc tcgagaagca gaacttaaga aagtttttga agaaagattg agttattatg
     1861 gaaaatctgg tgaggaggca agtgctcctt tggattatga gatgaaagcg cacgttagtt
     1921 atctttcaat gggagaaaag cggtttgttt ataataacgg tgagaatccc gtatccactc
     1981 agtatttgac tgatccgatt ttagtggtat tcacgccgac ttctacaggt gatagtttta
     2041 tatccctatc tagttggtct atcaatgctg gaaaacaact ctttatcaaa ggatatgaga
     2101 gtgggctaga acttttgaag aaagctggaa tttatgagca agtatcctat cttaaagaag
     2161 gaagaagtgt ttatctaact cgttataatg aagttcaaac tgaaacagca actttaatct
     2221 taggagctat tgtggggata gctagttcct tgttactatt ttattctgtc aatcttctat
     2281 atttcgagca attccgccga gatatcttga ttaaacgaat ttcaggttta cgattttttg
     2341 aaacacatgc tcagtatatg gttagtcaat ttgccagttt tgtatttggt gctagtcttt
     2401 ttattttaag cagtcgagac ttggtgattg gcttgctcac tttattagtc tttctagcta
     2461 gtgcagttct gacgctttac cgtcaagcgc agaaagaatc tcgtgtttct atgacaatta
     2521 tgaaaggaaa ataggatgat tgaactaaag aatatatcta aaaaatttgg aagccgtcag
     2581 ctattttcag atacgaatct tcattttgaa ggtgggaaaa tttatgcctt aatcggtaca
     2641 agtggctgtg gtaagacaac actcttgaat atgattggac gattagagcc atatgacaaa
     2701 gggcaaatca tctatgatgg cacttctctt aaggacatca agccttctgt tttctttaga
     2761 gattacttag gatacttatt tcaagatttt ggcttaattg aaagccaaac cgtcaaagag
     2821 aatctcaatc tgggtttagt tggtaaaaag ttgaaggaaa aagagaaaat ctctttgatg
     2881 aaacaagctc taaaccgtgt aaacctctct tatttggatt tgaagcaacc tatatttgag
     2941 ttatcaggag gagaagcaca acgtgttgca ttagcgaaga taattttaaa ggatccacct
     3001 ttgatcctcg cagatgaacc aaccgcttcc ttagacccca aaaactctga ggaattactt
     3061 tccatcctag aatctttaaa aaatccgaat cgaactatta ttattgcgac ccacaatcct
     3121 cttatttggg aacaagtgga ccaagttatt cgagttaccg atttatcaca tagatgatat
     3181 ggcaagattc agctagaaga aagagtcaca aacacacttt gtggcttttt tatttccata
     3241 aaaatggtaa aatagtagga gtagaaatgg agttcgagac atgaaagtaa tagatcaatt
     3301 taaaaataag aaagtccttg ttttaggttt ggccaagtct ggtgaatctg cagctcgttt
     3361 gttggacaag ctaggtgcca ttgtgacagt aaatgatggg aagcctttcg aggacaatcc
     3421 agctgcccaa agtttgctgg aagaagggat caaggtcatt acaggtggcc atcctttgga
     3481 actcttggat gaagagtttg ctcttatggt gaaaaatcca ggtatcccct acaacaatcc
     3541 catgattgaa aaggctttgg ccaagggaat tccagtcttg actgaggtgg aattggctta
     3601 tttgatttca gaagcaccga ttattggtat cacaggatcg aacggtaaga caaccacaac
     3661 gactatgatt ggggaagttt tgactgctgc tgggcaacat ggtcttttat cagggaatat
     3721 cggctatcca gctagtcagg ttgctcaaat agcgacagac aagaacacgc ttgttatgga
     3781 actttcttct ttccaactca tgggtgttca agaattccat cctgagattg cggttattac
     3841 taaccttatg ccaactcata tcgattatca tggattattt gaggaatatg tagcagccaa
     3901 gtggaatatc cagaacaaga tgacagcagc tgatttcctt gtcttgaact ttaatcaaga
     3961 cttggcaaaa gacttggctt ccaagacaga agccactgtt gtaccatttt caacacttga
     4021 aaaggttgat ggagcttatc tggaagatgg tcaactctac ttccgtggtg aagtagtcat
     4081 ggcagcgaat gaaatcggtg ttccaggtag ccacaatgtg gaaaatgccc ttgcgactat
     4141 tgctgtagcc aagcttcgtg atgtggacaa tcaaaccatc aaggaaactc tttcagcctt
     4201 cggtggtgtc aaacaccgtc tccagtttgt ggatgacatc aagggtgtta aattctataa
     4261 cgacagtaaa tcaactaata tcttggctac tcaaaaagcc ttatcaggat ttgacaacag
     4321 caaggtcgtc ttgattgcag gtggtttgga ccgtggcaat gagtttgacg aattggtgcc
     4381 agacattact ggactcaaga agatggtcat cctgggtcaa tctgcagaac gtgtcaaacg
     4441 ggcagcagac aaggctggtg tcgcttatgt ggaggcgaca gatattgcag atgcgacccg
     4501 taaggcctat gaacttgcga ctcaaggaga tgtggttctt cttagtcctg ccaatgccag
     4561 ctgggatatg tatgctaact ttgaagtacg tggcgacctc tttatcgaca cagtagcgga
     4621 gttaaaagaa taaaatatga aaaaaattgt ctttacaggt ggggggacgg ttggacacgt
     4681 taccctcaat cttttgttaa tgcccaagtt catcgaagat ggttgggaag tccactatat
     4741 cggggacaag cgtggtatcg aacaccaaga aatccttaag tcaggtttgg atgtcacctt
     4801 ccattctatt gcgactggga aattgcgtcg ctatttctct tggcaaaata tgctggacgt
     4861 cttcaaagtt ggctggggaa tcgtccaatc gctctttatc atgttgcgac ttcgtccaca
     4921 gacccttttt tcaaagggag gctttgtctc agtaccgcct gttatcgctg cgcgtgtgtc
     4981 aggagtgcct gtctttattc acgaatctga cctgtctatg ggcttggcca ataaaatcgc
     5041 ctataaattt gcgactaaga tgtattcaac ctttgaacaa gcttcgagtt tggctaaggt
     5101 cgagcatgtg ggagcggtga ccaaggtttc ggatcaaaaa aatccagaac cagatgaatt
     5161 ggtggatatt caaagccact ttaatcataa attgccgact gtattgtttg ttggcggttc
     5221 tgcaggtgct cgtgtcttta accaattggt gacagaccat aagaaagaac taacagagcg
     5281 ctacaatatt atcaatctaa ctggagattc tagcctgaat gagttgagcc aaaatctttt
     5341 tcgtgttgac tatgtgaccg atctctatca acccttgatg gaattggctg atattgttgt
     5401 gacacgtggt ggtgccaata cgatttttga gctcttggcg atagcaaaat tgcatgtcat
     5461 tgtgccactt ggtcgtgaag ctagtcgtgg tgaccagatt gaaaatgcag cttactttgt
     5521 taagaaaggc tatgcagaag accttcaaga aagcgatttg accttggata gtttggaaga
     5581 gaagctttct cacttactaa gtcacaagga agattaccaa gctaagatga aagcttctaa
     5641 ggaattgaaa tctctagcag atttttatca attgttgaaa aaagatttat cataaggaaa
     5701 gtaaatgtca aaagataaga aaaatgagga caaagaaatc ctcgaagaat tgaaagagtt
     5761 atcagaatgg cagaaacgaa accaagaata tctaaaaaag aaggctgaag aagaagcggc
     5821 tctagctgag gagaaggaaa aggaaagaca agctcggatg ggagaagaat ctgagaagtc
     5881 agaggacaaa caggaccagg agagtgaaac agaccaggaa gattcagaat cagctaagga
     5941 agagtctgaa gaaaaagtag catcctcaga ggctgacaaa gagaaagaag agaaagaaga
     6001 atcagagtct aaagagaagg aggaacagga taaaaagctt gctaaaaagg ctacaaagga
     6061 aaaaccagcc aaagcaaaga ttcctggtat ccatatcttg cgagccttca cgattttatt
     6121 tccaagtctg cttttattga ttgtctctgc ctacttgctc agtccttatg cgaccatgaa
     6181 agatattcgt gttgagggaa cggtgcaaac tacagctgat gatattcgac aggcttcagg
     6241 cattcaggat tcggattata cgattaacct tctgctagac aaggcaaaat atgaaaagca
     6301 gattaagtct aactattggg ttgaatcagc tcaacttgtc tatcaatttc caactaagtt
     6361 cactattaag gtcaaggaat atgatattgt ggcctactat atttctggtg aaaatcatta
     6421 tcctattctt tccagtggtc agcttgagac tagttctgtg agtctgaaca gtttaccaga
     6481 aacttattta tcagttctct ttaatgatag tgaacaaatc aaggtttttg tctcagaact
     6541 tgctcaaatt agcccagaac tcaaggcggc tatccaaaag gtggaattag ccccaagcaa
     6601 ggtgacatcc gatttaattc gattgaccat gaatgattcg gacgaagtct tggttcctct
     6661 atctgaaatg agtaagaaac tgccatatta cagtaagatt aagccacaat tgtcagaacc
     6721 gagtgtggtc gacatggaag ctggaattta cagttacact gtggcggata aattaattat
     6781 ggaggctgag gaaaaagcca aacaagaggc caaggaagct gagaaaaaac aagaagaaga
     6841 acagaaaaaa caagaggaag agagcaatcg aaatcaaaca aatcagcgtt catcgcgtcg
     6901 ctaggtttac cttttctctt atagttcttt agtgaccatg tttttacgtt taatatttga
     6961 catttgtttt tctttatgtt acatctgcaa tgtaatcgat tacaaaataa tttttgatga
     7021 agaaggtaac acatatgtca gatgaagtat tcttatttga agaaattgaa gaaatcgtag
     7081 cccctacaga tggtgaattt ttgggggaag ttttacttgg aactggggta gttctcttaa
     7141 ttggagtagc ctgttgttaa aaagataggg agtgataatc atgcaagata actttttatt
     7201 tgaggaaatt gaagaaattt cagtaccagt tagtgatttt tcagctggac ttgcaacagg
     7261 tatcggattt ggtttagcaa tccttgctct tgctggttgt tgaagtttgt tcatttacta
     7321 acatcaagct ttttcaattt cattttagac agtcatttga attttccgta ttagtcttgc
     7381 agcaagagat taatagaatt agtcattatt ttattgattg cggactgagg gactagagta
     7441 tgttttactt agcccctctt ttatttatta aaggttaggt ttgttatgag aattgttgat
     7501 aagattaaga tattacctac tccttatgag ggacagtatc atctatatat accatccagt
     7561 aagaaacatg tattagttgg gaaacaggaa aaaaatggtt agagcaacta ataggtcaag
     7621 aatttaccat
//
LOCUS       DAVLRT010000084         7617 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLRT010000084 DAVLRT010000000
VERSION     DAVLRT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.084"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            250..1695
                     /gene="cps4A"
                     /locus_tag="VXM85_001798"
     CDS             250..1695
                     /gene="cps4A"
                     /locus_tag="VXM85_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1346329.1"
                     /translation="MSRRFKKSSSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     IDQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVLEIDDSSLAVVKAAIQDVMEGR"
     gene            1697..2428
                     /gene="cps4B"
                     /locus_tag="VXM85_001799"
     CDS             1697..2428
                     /gene="cps4B"
                     /locus_tag="VXM85_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV1346330.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRIIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHVIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            2437..3129
                     /gene="cpsC"
                     /locus_tag="VXM85_001800"
     CDS             2437..3129
                     /gene="cpsC"
                     /locus_tag="VXM85_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1346331.1"
                     /translation="MKEQNTIEIDVFQLFKTLWKRKLMILIVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRDQGDKPGLTNQDLQAGSYLVKDYREIILSQNVLEKVATNLKLD
                     MPTKTLASKVKVTVPTDTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELLDTRVKRPEDVEDVLQIP
                     LLGVVPDLNKMK"
     gene            3139..3828
                     /locus_tag="VXM85_001801"
     CDS             3139..3828
                     /locus_tag="VXM85_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV1346332.1"
                     /translation="MPTLEISQAKLDFVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     LGEGKSTTSTNIAWAFAHAGYKTLLIDGDIRNSVMLGVFKARDKITGLTEFLSGTTDL
                     SQGLCDTNVENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRKCDASILVTEVGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSVDKYGSY
                     GNYGDYGKNKK"
     gene            3844..5211
                     /locus_tag="VXM85_001802"
     CDS             3844..5211
                     /locus_tag="VXM85_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1346333.1"
                     /translation="MNGKIVKSSLAIIQSFLVILLTYLLSAVREAEIVSTTAIALYIL
                     HYFVFYISDYGQDFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNEVVGEL
                     VAVSVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIREMAGLNVVTFSTTFYKTSHVIAKRIIDIMGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGCFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVLM
                     KDGAK"
     gene            5242..6414
                     /locus_tag="VXM85_001803"
     CDS             5242..6414
                     /locus_tag="VXM85_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV1346334.1"
                     /translation="MKQSVYIIGSKGIPAKYGGFETFVEKLTEYQKDGNIQYYVACMR
                     ENSAKSGFTADTFEYNGAICYNIDVPNIGPARAIAYDIAAVNKAIELSKGNKDEAPIF
                     YILACRIGPFISGLKKKIRSIGGRLLVNPDGHEWLRAKWSLPVRKYWKFSEQLMVKHA
                     DLLVCDSKNIEKYIREDYKQYQPKTTYIAYGTDTTPSSLKSEDAKVRNWYREKGVSEN
                     GYYLVVGRFVPENNYETMIREFIKSKSNKDFVLITNVEQNKFYDQLLKETGFDKDLRV
                     KFVGTVYDQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     DGAIYWKKDELAHVIEEVERFDEGDITELDEKSSQRIADAFTWEKIVSDYEEVFTV"
     gene            6411..>7617
                     /locus_tag="VXM85_001804"
     CDS             6411..>7617
                     /locus_tag="VXM85_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1346335.1"
                     /translation="MKILHYTLGFQPSRTGGLVKYAEDLMTQQATDGHEVIALYPGEI
                     SIIFRQVRIKKAENRKFQCYKIINSLPLALFGGISEPDEFMKSCDKASYRKFLKNIKV
                     DVIHIHSLIGLHKEFIEVAKELGIKTVFTSHDYYGLAPVPHFYFNGVDYSSNNTNLAW
                     NIMSSNALSVKKLRFFQVSFYPTIRKLMKMLGKNPKSKKNLVIRDITEAIDYSALRSY
                     YKAMFGLIDAYLFNSQLAKEVYEANHIIPKNNIIVSISNSSISNHNIRVSSGEKTVVA
                     YIGPDEEYKGYFDFLDIVNDLDQEKYDIKTYGHFPNEDCPPYIQQNGYFTKDTVGSVY
                     SNIDILIVPSRWKETFGLISIEALSYDVNVFVSKNVGSKDLVSEEHIFHNKDDLLKKI
                     QYVKFENVKF"
BASE COUNT         2429 a         1236 c         1648 g         2304 t
ORIGIN      
        1 aagattatac cacattgtgt actatattat attgaaacta gaatagtaca catctgcttc
       61 taaaatattg ttagaaaacg atttgactgt cctgatcaat ttgtcatgtt cttatttcat
      121 tttactatat ttttggttcg cgggaagtct actaagatac ttaaagatgc agatagtgaa
      181 aataaaggtg tagacattac cgtaaaaaag tgatataatc gtatgatgtt caatgtatag
      241 gtgttaatca tgagtagacg ttttaaaaaa tcaagttcac agaaagtgaa gcgaagtgtt
      301 aatatagttt tgctgactat ttatttattg ttagtttgtt ttttattatt cttaatcttt
      361 aagtacaata tccttgcttt tagatatctt aatctagtgg taactgcgtt agtcctacta
      421 gttgccttgg tagggctact cttgattatc tataaaaaag ctgaaaaatt tactattttt
      481 ctgttggtgt tctctatcct tgtcagctct gtgtcgctct ttgcagtaca gcagtttgtt
      541 ggactgacca atcgtttaaa tgcgacttct aattactcag aatattcaat cagtgtcgct
      601 gttttagcag atagtgagat cgaaaatgtt acgcaactga cgagtgtgac agcaccgact
      661 gggactgata atgaaaatat tcaaaaacta ctagctgata tcaagtcaag tcagaatacc
      721 gatttgacga tcgaccagag ttcgtcttac ttggcagctt ataagagttt gattgcaggg
      781 gagactaagg ccattgtctt aaatagtgtc tttgaaaata tcatcgagtc agagtatcca
      841 gactatgcat cgaagataaa aaagatttat accaagggat tcactaaaaa agtagaagct
      901 cctaagacgt ctaagaatca gtctttcaat atctatgtta gtggaattga cacctatggc
      961 cctattagtt cggtgtcgcg atcagatgtc aatatcctga tgactgtcaa tcgagatacc
     1021 aagaaaatcc tcttgaccac aacgccacgt gatgcctatg taccaatcgc agatggtgga
     1081 aataatcaaa aagataaatt aacccatgcg ggcatttatg gagttgattc gtccattcac
     1141 accttagaaa atctctatgg agtggatatc aattactatg tgcgattgaa cttcacttct
     1201 ttcttgaaaa tgattgactt attgggaggg gtagatgttc ataatgatca agagttttca
     1261 gctctacatg ggaagttcca tttcccagta gggaatgtcc atctagactc tgagcaggct
     1321 ctaggttttg tacgtgaacg ctactcacta gccgatggag accgtgaccg tggtcgcaac
     1381 caacaaaagg tgattgtggc tatccttcaa aaattaacgt caaccgaagc actgaaaaat
     1441 tatagtacga tcattaatag cttgcaagat tctatccaaa caaatatgcc acttgagact
     1501 atgataaatt tggtcaatgc tcagttagaa agtggaggga attataaagt aaattctcaa
     1561 gatttaaaag ggacaggtcg gacggatctt ccttcttatg caatgccaga cagtaacctc
     1621 tatgtgttgg aaatagatga tagtagttta gctgtagtta aagcagctat acaggatgtg
     1681 atggagggta gatgaaatga tagacatcca ttcgcatatc gtttttgatg tagatgacgg
     1741 tcccaagtca agagaggaaa gcaaggctct cttggcagaa tcctacaggc aaggggtacg
     1801 aatcattgtc tctacctctc accgtcgcaa gggcatgttt gaaactccgg aagagaagat
     1861 agcagaaaac tttcttcagg ttcgggaaat agctaaggaa gtggcgagtg acttggtcat
     1921 tgcttacgga gctgaaattt actacacacc agatgttctg gataagctgg aaaaaaagcg
     1981 gattccgacc ctcaatgata gtcgttatgc cttgatagag tttagtatga acactcctta
     2041 tcgcgatatt catagcgcct tgagcaagat cttgatgttg ggaattactc cagtcattgc
     2101 ccacattgag cgctatgatg ctcttgaaaa taatgaaaaa cgcgttcgag aactgatcga
     2161 tatgggctgt tacacgcaag taaatagttc acatgtcctc aaacccaaac tttttggcga
     2221 acgttataaa ttcatgaaaa aaagagctca gtatttttta gagcaggatt tggttcatgt
     2281 cattgcaagt gatatgcaca atctagacgg tagacctcct catatggcag aagcatatga
     2341 ccttgttacc caaaaatacg gagaagcgaa ggctcaggaa ctttttatag acaatcctcg
     2401 aaaaattgta atggatcaac taatttagga gaaatgatga aagaacaaaa cacgatagaa
     2461 atcgatgtat ttcaattatt taaaaccttg tggaaacgca agctaatgat tttaatagtg
     2521 gcacttgtga caggtgcggg ggcttttgca tatagcactt ttattgttaa gccagaatat
     2581 acgagtacca cgcgaattta cgtagtgaat cgcgatcaag gagacaagcc ggggctgaca
     2641 aatcaggatt tgcaggcagg atcttatctg gtaaaagact accgtgagat tatcctttcg
     2701 cagaatgtat tggaaaaagt agcgacaaat ttgaagttgg atatgccaac aaaaacgtta
     2761 gccagcaaag ttaaagtgac tgtaccaact gacactcgta tcgtctcaat ctctgtcaaa
     2821 gataaacaac cagaagaagc cagtcgtatc gctaattctc tacgagaagt tgctgcagaa
     2881 aagatcgtcg ctgtaacgcg agtatctgat gtaacgacac ttgaagaagc gcgaccagct
     2941 acgactccct cttctccaaa tgttcgacgc aattccttgt ttggttttct tggaggagca
     3001 gtcgtaacag taattgctgt tcttttgatt gagttgctcg acacccgtgt gaaacgtcct
     3061 gaagatgtcg aagatgtact gcaaattcca cttttagggg tcgttccaga tttgaacaaa
     3121 atgaaatagg aggaagttat gccaacatta gaaatctcac aggcaaaatt ggattttgta
     3181 aaaaaggcag aggaatatta taacgctttg tgcacgaacc tacagttaag tggagatgat
     3241 ttgaaagtat tttctatcac ttctgtgaaa ctaggagaag gaaaatcaac gacttccacc
     3301 aatatcgctt gggcttttgc gcatgcaggt tacaaaacgc tgctgattga tggagatatt
     3361 cgcaattctg ttatgttagg tgtctttaaa gcaagggata agattacagg cctgacagaa
     3421 tttttatcag gaactacaga cctatcacaa gggctttgtg ataccaatgt cgaaaatctc
     3481 tttgtaattc aggctggctc tgtgtcaccg aatccgacag ctcttcttca aagtaagaat
     3541 ttcagtacaa tgcttgaaac cttgcgtaaa tattttgact acatcattgt agatactgct
     3601 cctgtcggtg tcgtgattga tgcggctatt attacgcgaa aatgcgatgc ttctatttta
     3661 gtgacggagg taggtgaaat aaatcgacgg gatattcaaa aagcaaaaga acagttggaa
     3721 cacacaggaa agccgttttt gggagttgtg ttgaataaat tcgatacttc agtagacaaa
     3781 tacggttctt atggaaatta tggagattac gggaaaaata aaaaataggt cgggggatta
     3841 gagatgaatg gaaaaatagt aaagtcttca ttggccataa tccagagttt tcttgttatt
     3901 ttattgactt atctacttag tgctgtgaga gaagcggaga ttgtttcaac aacagctatt
     3961 gcactttata tcctccatta ttttgtcttt tatatcagtg attatggaca ggatttcttt
     4021 aaaagaggat atttgattga acttgtccag acattgaaat atatcctatt ctttgcacta
     4081 gcgattagta tttctaattt tttcttagag gatcgattta gtatttccag acgaggcatg
     4141 atttactttc tcacattaca tgctctctta gtctatgtgc taaacctatt tatcaagtgg
     4201 tattggaagc gggcttatcc caactttaaa ggaagtaaga agattctcct acttacagca
     4261 acttctcgtg tcgaaaaggt actggataga ttaatagaat caaatgaggt tgttggggag
     4321 ttggtagccg tcagtgtctt agataaacca gattttcagc atgattgttt aaaggtagta
     4381 gcagaggggg agatagtaaa ctttgcgact catgaggtgg tcgatgaagt ctttatcaat
     4441 cttccaagtg aaaaatacaa tattggagag cttgtctctc agtttgaaac gatgggaatt
     4501 gatgtaacag ttaatctaaa tgcttttgat cgtagtttgg cacgtaacaa gcaaattcgt
     4561 gagatggcag gattaaacgt tgtgactttt tctacaacat tttataagac tagtcatgta
     4621 attgctaagc ggattattga tatcatgggt gcattggtag ggctgatact atgtggttta
     4681 gtcagtattg tactggttcc tttgattcga aaggatgggg gctctgctat ttttgctcag
     4741 acgcgtatag gaaaaaatgg tcgtcagttc actttttata agtttcgctc tatgtgtgta
     4801 gatgccgagg cgaaaaaaag agaactcatg gaacaaaata ccatgcaggg tggaatgttt
     4861 aaggtggacg atgatcctcg tatcacgaaa attggttgtt ttatacggaa gactagcttg
     4921 gacgagctac cacagtttta taatgttcta aagggagata tgagtttggt tggcacacgg
     4981 ccaccaacag tggacgagta tgagcactat accccagaac aaaagcgtcg tctgagtttt
     5041 aaacctggca taacagggtt atggcaggtc agcggacgaa gtgagattaa gaattttgat
     5101 gaagttgtca aattagatgt agcctatata gacgattgga caatttggaa agatattgaa
     5161 attttattga agacagttaa agttgtattg atgaaggatg gagcgaagtg atttttgccc
     5221 ttcggtcttg ttaggagaga tatgaaacag tcagtttata tcattggttc aaaggggatt
     5281 cctgccaagt atggaggatt tgaaaccttt gttgagaaat taacagaata tcaaaaagat
     5341 ggtaacatcc aatactatgt tgcttgcatg cgcgaaaatt ctgcaaagtc aggatttaca
     5401 gcagacacat ttgagtacaa cggtgctatt tgttacaaca ttgatgtgcc taatattggt
     5461 cctgctagag ccattgctta cgatattgca gcggtcaata aggctattga attgtctaag
     5521 ggaaacaagg acgaggctcc cattttttac attctagctt gtcgtatcgg accttttatt
     5581 tctggactta agaaaaaaat tcgttcgatc ggaggccgtt tgctggtaaa tccagatggt
     5641 catgagtggc ttcgagctaa atggagcttg ccagttcgga agtattggaa attttcggaa
     5701 cagttgatgg tcaaacatgc agatttatta gtctgcgata gcaaaaatat cgaaaaatat
     5761 attcgagagg actataaaca gtatcagccc aagacgacct atatcgctta tggtacagat
     5821 actacccctt caagtctgaa atcagaagat gccaaagttc gaaactggta tcgtgaaaag
     5881 ggagtaagcg aaaatggcta ttatctagtg gtgggacgat tcgttcccga aaacaactat
     5941 gaaaccatga ttcgtgaatt tatcaagtct aagtccaata aggactttgt ccttattaca
     6001 aatgtggaac agaataaatt ttacgatcag ttgctcaagg aaacaggttt cgacaaggat
     6061 ctaagagtca aatttgtggg aacagtctat gaccaagaat tgctcaagta tatccgtgaa
     6121 aatgctttcg cctatttcca tggacatgaa gttgggggta ccaatccatc gcttcttgaa
     6181 gctttagcat ccactaaact caacttgttg ttggatgtcg gctttaaccg tgaagttggt
     6241 gaagacggag ccatttattg gaaaaaagat gagctagcgc atgtaatcga agaagtggaa
     6301 cgatttgatg aaggtgatat tacggaatta gacgaaaagt ctagccaacg aattgcggat
     6361 gcatttacgt gggaaaagat tgtgtcagat tatgaggaag tgtttactgt atgaaaattt
     6421 tgcattatac tttaggcttt caaccatcac gtacaggtgg tcttgtcaag tatgctgagg
     6481 atttgatgac tcaacaagct acagatggac atgaagtaat cgcattatat ccaggagaaa
     6541 tctcaattat atttaggcag gttagaataa aaaaagcaga gaatcgaaaa ttccaatgct
     6601 ataaaataat aaacagccta cccttggctc tatttggtgg tatttcagaa ccagatgaat
     6661 ttatgaagag ttgtgataaa gcttcttata ggaaattttt gaaaaatatt aaggtagatg
     6721 taattcacat tcactcgctt ataggattac ataaggaatt tattgaagtt gcaaaagaat
     6781 taggaattaa aactgtattt acaagccatg attattatgg tttagcgcca gttccgcatt
     6841 tttattttaa cggagtagat tatagtagta ataatactaa tttagcatgg aacatcatgt
     6901 cctctaatgc tcttagtgta aaaaaacttc gattctttca agtctctttt tacccaacaa
     6961 ttcggaaatt gatgaaaatg cttgggaaaa atccaaagtc caagaaaaac ttagtaattc
     7021 gtgatatcac ggaggctatt gattatagtg ctctccgctc ttattataaa gcaatgtttg
     7081 ggttaataga tgcttacctt ttcaatagtc agttagctaa ggaagtttat gaagctaatc
     7141 atataatacc taaaaacaat atcatagtgt ccatttctaa cagctcaata agtaatcata
     7201 atattcgtgt tagttccggt gagaaaacag tggtagctta tattgggcct gatgaggaat
     7261 ataaggggta ttttgatttt ctagatatag ttaatgattt agatcaggaa aagtatgata
     7321 ttaaaacata tggacatttt ccaaatgaag attgtccacc ttatatccaa cagaacggtt
     7381 atttcacaaa agacacggtt ggaagcgttt attccaatat agatattttg attgtgccga
     7441 gtaggtggaa ggaaacattt ggtttgatta gtatagaggc gctatcgtat gatgtgaatg
     7501 tttttgtgag caaaaatgtg ggctctaaag atttggtttc agaagaacat atctttcata
     7561 ataaagacga tttgttaaaa aaaattcaat atgtaaaatt cgagaatgta aaattca
//
LOCUS       DAVLRT010000085         7246 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLRT010000085 DAVLRT010000000
VERSION     DAVLRT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.085"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            1..550
                     /locus_tag="VXM85_001805"
                     /pseudo
     CDS             1..550
                     /locus_tag="VXM85_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            717..917
                     /locus_tag="VXM85_001806"
     CDS             717..917
                     /locus_tag="VXM85_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346336.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKSVLLDAQKAPEDSALTE"
     gene            959..1363
                     /locus_tag="VXM85_001807"
     CDS             959..1363
                     /locus_tag="VXM85_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346337.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1594..2301
                     /gene="ccdA2"
                     /locus_tag="VXM85_001808"
     CDS             1594..2301
                     /gene="ccdA2"
                     /locus_tag="VXM85_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1346338.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDFNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2312..2869
                     /gene="sdbB"
                     /locus_tag="VXM85_001809"
     CDS             2312..2869
                     /gene="sdbB"
                     /locus_tag="VXM85_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1346339.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3243..4634
                     /locus_tag="VXM85_001810"
     CDS             3243..4634
                     /locus_tag="VXM85_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1346340.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4777..5694
                     /gene="adcAII"
                     /locus_tag="VXM85_001811"
     CDS             4777..5694
                     /gene="adcAII"
                     /locus_tag="VXM85_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1346341.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLAEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLEADPQNDKTYLENLEENMSVLAEELK"
     gene            5702..>7246
                     /gene="phtB"
                     /locus_tag="VXM85_001812"
     CDS             5702..>7246
                     /gene="phtB"
                     /locus_tag="VXM85_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1346342.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVLKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAQAYWNGKQGSRPSSSSSYNANTAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDISSDKVKLVEDILAFLAPIRHP
                     ERLGKPN"
BASE COUNT         2252 a         1331 c         1520 g         2143 t
ORIGIN      
        1 acgtaatacc atttatggct ggttaaagct aaaagagaaa acaggagagt taaaccacca
       61 agtaaaagga acaaaaccaa gaaaagttga tagagataga cttaaaaact atcttactga
      121 caatccagac gcttatttga ctgaaatagc ttctgaattt ggctgtcatc caactaccat
      181 ccactatgcg ttcaaagcta tgggctacac tcgaaaaaaa gaaccacacc tactatgaac
      241 aagacccaga aaagtagcct tatttcttaa gaattttaat agtttaaagc acctagcacc
      301 tgtttagatt gatgaaacag gattcgatac ttatttttat cgagaatatg gtcgctcatt
      361 aaaaggtcag ttaagaagag gcaaagtatc tggaagaaga tatcagagga tttctttggt
      421 tgcaggtcta acaaatggtg aattaatcgc tccaatgact tacgaagaga tgatgacgag
      481 cgactttttt gaagtatggt ttcagaagtt tttcttacca acattaacca caccatcggt
      541 tattatagta aaatgaaata agaataggac aaatcgatca ggacagtaaa atcgaattct
      601 aacaatgttt tagaagtcca tatgtactat tctagtttca atataccata actgttccta
      661 gtcaacctgc aagccctgtc ccagctccaa gctcagctcc gactcaagat cctagagtgg
      721 tagcaagcgc ttcggctagc tcaacgagca cacaagctca agagcaagtt gacaagtctg
      781 aacttcgtgc cttgagtcaa gagttagacc aacgcttgaa agctttggca acagtatctg
      841 atccgaaaat cgatgcaacc aagtctgtcc tcctagatgc tcaaaaagct ccggaagata
      901 gtgccttgac agagtaaggt ctgcatactg cagtagagtc tgtgaaggct gggctgcctt
      961 ggactctctc aaagatgtga aagcaaatgc tagcgacagc aagcctgcac aggacaagaa
     1021 ggatgcaaaa caaggaacgg aagatagtaa ggattcagat aagatgactg aaacaaactc
     1081 agttccggca ggagtgattg tggtcagtct acttgccctc ctaggcgtga ttgccttctg
     1141 gctgattcgc cgtaagaaag agtcagaaat ccagcaatta agcacggaat tgatcaaggt
     1201 tctaggacag ctagatgcag aaaaagcgga taaaaaagtc cttgccaaag cccaaaacct
     1261 tctccaagaa acccttgatt tcgtgaaaga agaaaatggc tcagcagaga cagaaactaa
     1321 actagtagag gagcttaaag caatccttga caaactcaag taaaacatga tttatgaaat
     1381 gtcagaccta aaagacattt tcgttcacaa aagcagcgat gagtaatcct tgtcgttgct
     1441 ttttggctat tttgggggat aagatccgcc ttgactagca aaagaaaaga ttggtccaat
     1501 agtgcccgat acgattttgc gtagcagaaa gcttacaaat aaaattgaat atagtaaaat
     1561 aggattagaa ttattaagaa agttggttct ttattggaaa cgatagtatt tttaatctct
     1621 gtttttctag caggtgtttt atcctttttt tctccttgta tttttcctct tctgccagtc
     1681 tatgctggga ttttattgga tgatcaagaa agtgcaaaaa gcttttcttt gtttgggaga
     1741 aaggttctct ggtcaggctt gattcgaaca ctttgcttta tcgctggtat ctctctcatt
     1801 ttctttattc taggctttgg tgctggttac tttggtcata ttctctatgc aaattggttt
     1861 cgatatgtca tgggagctat tattatcatt ttgggtcttc accagatgga aatttttcat
     1921 ttgaagaaat tagaagttca aaaaagtttt acctttaaaa aatcagattt taatcgttat
     1981 tggtcagctt ttttacttgg tattaccttt agctttggtt ggacaccttg tattggtcca
     2041 gttttaagtt ctgttttagc acttgcggct tctggaggca atggtgcttg gcaaggagcg
     2101 atttatactc tcatttacac tctgggcatg gcccttcctt tcttggtctt ggcactagct
     2161 tcaggtctag tcatgccata ttttagtaaa atcaagcgtc atatgatgct actaaagaaa
     2221 attggtggtt tcctcattgt tttaatggga attttgttac tattaggaca agtaaatgtt
     2281 ctagctggaa tttttgaata aaggaggaca aatgaaaaaa gtaatgtttg ctggcttaag
     2341 tctcttgtca ttagttgtat tgatggcctg tggtgaggaa gaaactaaaa agactcaagc
     2401 agcacaacag ccaaaacaac aaacgactgt acaacaaatt tctgttggaa aagatgctcc
     2461 agactttaca ttgcaatcca tggatggcaa agaagttaag ttatctgatt ttaagggtaa
     2521 aaaggtttac ttgaagtttt gggcttcatg gtgtggtcca tgcaagaaaa gtatgccaga
     2581 gttgatggaa ctagcggcga aaccagatcg tgatttcgaa attcttactg tcattgcacc
     2641 aggaattcaa ggtgaaaaaa ctgttgagca attcccacaa tggttccagg aacaaggata
     2701 taaggatatc ccagttcttt atgataccaa agcaaccacc ttccaagctt atcaaattcg
     2761 aagcattcct acagaatatt taattgatag ccaaggaaag attggaaaga ttcaatttgg
     2821 tgctatcagt aatgcggatg cagaagcagc atttaaagaa atgaactaga atcgataaaa
     2881 tctgattaca agatgtagtc agattttggt aacttctaaa actaacttcc agtttcccac
     2941 aacctagctt ttagtatgag aaaaacgcgt gacatcagtg gaaatccgtc ttagactaac
     3001 ttccactggt tttctttttc ttgatatata agggttcaaa agcgaaaaga ctcagaaaaa
     3061 gtgcacgaca aatagcccta aaacagagtc gtcttagagt aacttccagt tttgggagag
     3121 agatggaagt tactttgaga agttacggta gtcagatttt tttagaaaaa cattttataa
     3181 atattcacat atctcctata tttatggtaa aatagaatta tcagtttatt ttggagtcaa
     3241 agatgaatat atttagaaca aagaatgtta gtttagataa aacagagatg cataggcatt
     3301 tgaagttatg ggatttgatt ttgctgggta tcggagccat ggtagggaca ggcgtcttta
     3361 caatcacagg tactgcagct gcaacacttg ctggcccagc cctagtgatt tcaatcgtta
     3421 tttctgcctt gtgtgtggga ttatcagccc tcttttttgc agaatttgcc tcgcgagtac
     3481 ccgctacagg aggtgcctat agttacctct atgctatctt aggagaattc cctgcctggt
     3541 tggctggttg gttaaccatg atggagttca tgacagccat atcaggcgta gcttcgggtt
     3601 gggcagctta ttttaaaggc ttgctctctc aatacgggat agcctttcct caggctttaa
     3661 atggtacctt taatcctcaa gcagggacat ttgttgattt attgcctatt cttgtcttgg
     3721 tcttggtgac ttcgcttgtt ttactaaatg ccaaggcagc cttacgcttt aattcgattc
     3781 tagttatttt gaaattttcc gctttagctc tctttgtttt agtaggaatt tggaatatta
     3841 agtttgataa ttggagcaat tttgctcctt acggttttgg acaaatctat ggagctagta
     3901 ctggtattat ggctggtgca tccttgatgt tcttcggttt tttgggattt gaatccatct
     3961 ctatggctgt agatgaagtc aagactcctc aaaaaaatat tcctagaggg attgtcttat
     4021 cgctttctat cgtaaccatt ctctatgcct tggtgaccct tgttttgact ggtgtggttc
     4081 actatagtca tttaaatgtc gatgatgccg ttgcctttgc ccttcgtagt gttgggatta
     4141 gttgggcagc caactatgtg tcattagtgg ctatcttgac cttgattaca gtttgtattt
     4201 cgatgaccta tgccctatcg cgtatgattt acagtttagc acgtgacggc ttagtgcctg
     4261 ctgcctttaa ggaactgacg aagactagca aggtaccaaa gaatgctact atcttgacag
     4321 gtctagcttc agcagtagca gcaggaatgt tcccactagc cagtattgca gcctttttaa
     4381 atatttgtac cttagcctac ttgatcatgt tggcttatgg tctgattcgc ttacggaaag
     4441 aaaaaggaat gcccaaagct ggggaattta aaacaccatt ggtacctctg ttaccaattt
     4501 tatcgatcat catttgttta tccttcatgt tacagtataa tatgaatacc tggattgctt
     4561 tcctagttgc gttgttagta ggaagtatta tttacttcac ttatggctat aagcattcta
     4621 ccatagaaga ataaagtgaa agtctattga gttgttgaca ctcagcagat ttttatatgt
     4681 gaaagaaatt tcaaattttt tctaaaaata tcttgacaga ttaaattttc aggagtagaa
     4741 tatttactag ttaattaaat ggttaaggag ttgttcatga agaaacaaaa tttattttta
     4801 gtcctgttaa gtgtctttct tttgtgcttg ggggcttgtg gtcaaaagga aagtcagaca
     4861 ggaaagggga tgaaaattgt gaccagtttt tatcctatct acgctatggt taaggaagta
     4921 tctggtgact tgaatgatgt tcggatgatt cagtcaagta gtggtattca ctcctttgaa
     4981 ccttcggcaa atgatatcgc agccatctat gatgcagatg tctttgttta ccattctcat
     5041 acactcgaat cttgggcagg aagtctggat cccaatctaa aaaaatccaa agtgaaggtc
     5101 ttagaggctt ctgagggaat gaccttggaa cgtgtccctg gactagagga tgtggaagca
     5161 ggggatggag ttgatgaaaa aacgctctat gaccctcaca catggctaga tcctgaaaaa
     5221 gctggagaag aagcccaaat tatcgctgat aaactttcag aggtggatag tgagcataaa
     5281 gagacttatc aaaaaaatgc gcaagccttt atcaaaaaag ctcaggaatt gactaagaaa
     5341 ttccaaccaa aatttgaaaa agcgactcag aaaacatttg taacacaaca tacagccttt
     5401 tcttatctag cgaagagatt tgggcttaat caacttggta ttgcaggcat ctctcctgaa
     5461 caagaaccaa gtccacgaca attagcagaa attcaggaat ttgttaaaac ctataaggtt
     5521 aaaacgattt ttacagaaag taatgcttct tcaaaagtag ctgaaactct tgtcaaatca
     5581 acaggtgtgg gtcttaaaac tctgaatcct ttagaggcag acccacaaaa tgacaagacc
     5641 tatttagaaa atctggaaga aaatatgagt gttctagcag aagaattaaa gtgaggaaag
     5701 aatgaaaatc aataaaaaat atctagcagg ttcggtggca gttcttgccc taagtgtttg
     5761 ttcctatgag cttggacgtt accaagctgg tcaggataag aaagagtcta atcgagttgc
     5821 ttatatagat ggtgatcagg ctggtcaaaa ggcagaaaac ttgacaccag atgaagtcag
     5881 taagagggag gggatcaacg ccgaacaaat cgtcatcaag attacggatc aaggttatgt
     5941 gacctctcat ggagaccatt atcattacta taatggcaag gtcccttatg atgccatcat
     6001 cagtgaagag ctcctcatga aagatccgaa ttatcagttg aaggattcag acattgtcaa
     6061 tgaaatcaag ggtggttatg tccttaaggt aaacggtaaa tactatgttt accttaagga
     6121 tgcagctcat gcggataata ttcggacaaa agaagagatt aaacgtcaga agcaggaacg
     6181 cagtcataat cataactcaa gagcagataa tgctgttgct gcagccagag cccaaggacg
     6241 ttatacaacg gatgatgggt atatcttcaa tgcatctgat atcattgaag atacgggcga
     6301 tgcctatatc gttcctcacg gcgaccatta ccattacatt cctaagaatg agttatcagc
     6361 tagcgagtta gctgctgccc aagcctattg gaatgggaag cagggatctc gtccttcttc
     6421 aagttctagt tataatgcaa atacagctca accaagattg tcagagaacc acaatctgac
     6481 tgtcactcca acttatcatc aaaatcaagg ggaaaacatt tcaagccttt tacgtgaatt
     6541 gtatgctaaa cccttatcag aacgccatgt ggaatctgat ggccttattt tcgacccagc
     6601 gcaaatcaca agtcgaaccg ccagaggtgt agctgtccct catggtaacc attaccactt
     6661 tatcccttat gaacaaatgt ctgaattgga aaaacgaatt gctcgtatta ttccccttcg
     6721 ttatcgttca aaccattggg taccagattc aagaccagaa caaccaagtc cacaatcgac
     6781 tccggaacct agtccaagtc tgcaacctgc accaaatcct caaccagctc caagcaatcc
     6841 aattgatgag aaattggtca aagaagctgt tcgaaaagta ggcgatggtt atgtctttga
     6901 ggagaatgga gtttctcgtt atatcccagc caaggatctt tcagcagaaa cagcagcagg
     6961 cattgatagc aaactggcca agcaggaaag tttatctcat aagctaggag ctaagaaaac
     7021 tgacctccca tctagtgata gagaatttta caataaggct tatgacttac tagcaagaat
     7081 tcaccaagat ttacttgata ataaaggtcg acaagttgat tttgaggctt tggataacct
     7141 gttggaacga ctcaaggata tctcaagtga taaagtcaag ttagtggaag atattcttgc
     7201 cttcttagct ccgattcgtc atccagaacg tttaggaaaa ccaaat
//
LOCUS       DAVLRT010000086         7224 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLRT010000086 DAVLRT010000000
VERSION     DAVLRT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.086"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..232)
                     /locus_tag="VXM85_001813"
     CDS             complement(<1..232)
                     /locus_tag="VXM85_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HEV1346343.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKI"
     gene            complement(532..2226)
                     /locus_tag="VXM85_001814"
                     /pseudo
     CDS             complement(532..2226)
                     /locus_tag="VXM85_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(2329..3314)
                     /locus_tag="VXM85_001815"
                     /pseudo
     CDS             complement(2329..3314)
                     /locus_tag="VXM85_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            complement(3417..3578)
                     /locus_tag="VXM85_001816"
     CDS             complement(3417..3578)
                     /locus_tag="VXM85_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346344.1"
                     /translation="MKEREVLTGQRLNELEINGIRLTKFKNGEIGIEFIWIDTENPPS
                     DAIGWVAKK"
     gene            complement(3600..4355)
                     /locus_tag="VXM85_001817"
     CDS             complement(3600..4355)
                     /locus_tag="VXM85_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1346345.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSLEDLY"
     gene            complement(4426..7077)
                     /locus_tag="VXM85_001818"
     CDS             complement(4426..7077)
                     /locus_tag="VXM85_001818"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1346346.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWDEFADWYVELTKEVLYSDNEEDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELDRLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(7089..>7224)
                     /locus_tag="VXM85_001819"
     CDS             complement(7089..>7224)
                     /locus_tag="VXM85_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1346347.1"
                     /translation="ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN
                     "
BASE COUNT         2154 a         1605 c         1260 g         2205 t
ORIGIN      
        1 ttattttttc ggtgatgtac accaactcca aaggaacgtt aataaacatc tctgtaaaac
       61 gggtcatatt atctctattt ttgagaaaaa aacttttctc aatatcaatt ggatttactt
      121 tatctccagt ttttcttcca aaagctattc cccttccaaa aagaagaata tcgatatctt
      181 ttttactttg cgcaattacg gcattgtggt ttagtattcg ttttatgatc ataaaatttc
      241 ccttttaaaa taaaaggaac ctacttaaaa acatcaagaa acttcttctg atgtgattaa
      301 ataggttcct gctcacttgt attagtgata acatcctttt atttcaattt ttaatggtga
      361 catgaagctt aaataattaa taactctgta accgttttta ttttatgata tttcaatcta
      421 tttgtcaagt acttataaaa atttttttat tttgtgtgat tcgaatgtga taatggcaac
      481 agtctcacta aagaaatgaa aatcatacat agaaattttt aaaaattttc tctatcgtaa
      541 acctgccact agttcagaaa ctatactagc tgattccaaa cgtaatatgg tcatctcatc
      601 taccttccct aataatggca ttgcaccata ccaaacaata gtcttattca ccaaaagaat
      661 aggcgttact atttttgatt gagtaaaaga cactttaatt ttctggctct tcaactggtc
      721 tagccatgct ttatttgcaa ccgtctctgg tatacatatc tcaatttgac taatcctaaa
      781 ttcttttaga aaattcatca gttttgtctg gtgcacataa ggtaaaataa gcagacattc
      841 ctgtctttcc cctgctaagt cctctctcaa caccttctca tatctactat caacataaac
      901 gaattgtttc tcctcaccct caatggcacg ataatccatc tttcgataag ctacttgtcg
      961 tttctgaaac atcttttcta aataaggaac atgaatatcc acataatcga aaatacgcac
     1021 caaagactta tccttgtagt ttctatgaat ccaacctgca tactgaatca aattattttt
     1081 ccaagaaaag ggtgctgcca agataagcgt gtccaactga ggtaagtcaa aaccttcgcc
     1141 aatgtatttt ccagtagaca acaaaacaaa ccctttatct aactgttcta acgtctccag
     1201 taaactcgtt ctctctcgga ctttggtttt tccgctaata atgtaacagt catcaacctc
     1261 tttctctttc aataattttt caaagacatc tatctgttga attcgattaa ctaaaaccaa
     1321 gatattccgt ccttctgcca cttgggctag aatatccttg agaatcatct gattcctcac
     1381 tgagtcagta gcaatccaat cactaagctg tataaaatta cttgctttgg tcttttcaat
     1441 ttccaaatga ccaaaagaag tgaatcttaa ttgcaattgc cgtttaaaat ccgtttccct
     1501 cttatcagca gtatggagta tctcaccaat tctctgaaaa acaataggct catgaccatt
     1561 cttacgctca ggcgtagccg tcaaaccgta aagatacttc cctctaaact gagcaacaac
     1621 tttttcaaac atcaaggcag agacatgatg acactcatcc acaatcatca tctcatactc
     1681 atccaaaaga ctttgactat tttccaactt aaatagagat tgaatcataa cgacatcaac
     1741 cagtttactc agccatttct tagtcccacc gtactgccca acatagccga ttaccttttc
     1801 acgacctgat gccgtataac ggatagcctc ctcctcttcg aaagtcaaaa agcagtttaa
     1861 gcgatctagc cattggtcta agagttgctt attatggact agaataattg tttttgtttt
     1921 ccgttcagag ataagagcag cacctaaaac ggtcttccca aaaccagtct ccgcatgaag
     1981 taaaccattt tctttagaag tcatatctga cagggctaac tcttgctcaa aagtgagttc
     2041 tcccttaaat tccacgctaa tagacctttg tacctttctc ctatcttcca cagatacctg
     2101 cttaaattta tcttgcaatg gatacagcaa acctcttggc aaccataaat aatgatcgga
     2161 ttctccaaat aaatacattc tctcaggaat ttgataggtt ggctgtcgca tagcctgctt
     2221 taaatatagt aaaatgaaat aataacagga caaatcgatc aggaaagtca aatcgatttc
     2281 taacaatgtt ttagaagcag aggtgtacta ttctagtttc aatctactat aaaattcggg
     2341 attagaaaag gaagccatat ttttcaagaa aaaagtgcct tggatgtcac agacgatttt
     2401 tcaagttgga tcatattgga taaaacgacc ttcaactcct tatccaattc ttgcttgcct
     2461 aactcctctt ggattaacag tgccacttta gcagttgaaa tcctctgaat ttcttgtaga
     2521 tacctccatt ggtcttcata aggctgaaac tgttcatcca caaagaccgt tcgcccttga
     2581 tggtaagctt ctccttgaaa aggcaaggca atcaaatttc caaatccccc cttaggaagg
     2641 acatcctgat ttggaaacat gcgatcaaaa gaatcaaagg acagttgcat actttcctgc
     2701 attgccaatt ctaatagttt ctttccaaac aggcgagcct ttcgactcag aatcgcttcc
     2761 tcaaagaaga accaaatatg gagtccgtaa cccgaacgag aaatctctaa atgggcttcc
     2821 atctggcgtt ccctggctat tcttcgaatg gttaaaccag cttctttcca atctccttca
     2881 tccaaatcca gtaccaaaaa ataacagcta tcatctaagt gcataggaaa gataccgata
     2941 gctgcctccc cacgaaaatg agatttcaaa acggaatccg tcaatgtctg gaaactccgt
     3001 ttttcaggta gcaactgctt ccaaccataa tcataggaag gaaaatactg aattttccct
     3061 tgatcattga taaaactctt agcataaaca tcataacggc cagcaaacac agaggtaaac
     3121 aattgtaatt tttcttgcgt cgtcaagcga ctactttgaa gttggaacat ctcctcgaac
     3181 tgagagcgtt ccataacaaa ctgactcttg tctttaagac gtttacacaa gaaaaactca
     3241 ctatccccat caaaagaaga aaagacatca agtacttcta ccacaattcc atccaaagac
     3301 acaaaaacag ccataagagt cacctccttg attcctatag gctgattata acaagaatag
     3361 ctgaaattgt acacgaaaat aaaatccctt atttgttaca atacctttga aaacttttat
     3421 ttctttgcta cccagccgat ggcatcgctt gggggatttt cggtatcaat ccagataaat
     3481 tcaataccga tttccccatt tttaaattta gttaaacgaa taccgttgat ttccagttca
     3541 ttgagtcgtt gacctgtcaa gacttctcgt tctttcaact ggaaaagtta aggggggaac
     3601 taatacaaat cttctaagct tctatattca atgatttcat ttttgataat attcttttta
     3661 aactcaaaat gttttaaagg attatcgatt aacactaact ttggaatatc atctagattt
     3721 gtgaccaagg ataaaataaa atccgattga aactccttga ctttttcaaa ttcttttgca
     3781 gttaaacgca cacgcccctt agattttcgg atacctttta cttcggcaag gtaagaatac
     3841 tcttggacat ctacctgaaa gtcatatcca tcaccataaa gacgggcatc ggttaactgt
     3901 ccaccttgga atttttcttc tttatcaaaa tgaaggataa aataattctc agcctccatt
     3961 cccgtttcct gtagacgttt gaatttagtt cgaactattg gtttttcaac gatggttatg
     4021 cctgttttct tcccttcact ggcaagtaac attttaacta tttcagcata actatgaaca
     4081 tcttcatttc caaacatcat atcaattaaa tctttacgaa aacgataaac ttcagctttc
     4141 tgccaccaac cttttcgatt attgtcaaaa taaggatcaa ataaatccat tctatttttt
     4201 acaactccag ttgtttcagc aattcccaga gaaaccacat aacgataaaa ctccgacttg
     4261 ctagagcatt gaaattcttt tataaaaagc ttgtcaaact tggcaagacc gtagccaatt
     4321 aaatttaaca attcataatg agggtgtttg gacatcactt tctccatcaa tagatattga
     4381 tttcattata ccaaaaaaac acggctcatc accgtgtttc tgtgtttatt taacgagttt
     4441 cttcatctca tcaatacgtg ctacggtcgc atcatacttg gcttggtagt cagcttgttt
     4501 gtcgcgttct ttttggacga cttctggttt ggcgttggct acgaagcgtt cgttagagag
     4561 tttcttacca accatgtcca gttctttttg ccatttagcg agttctttgt cgagacgatc
     4621 tagttcttct tcaacattga ggagatcggc cagtggcagg tagatttctg ctcctgtgat
     4681 gacacttgac atagccagtt caggtgcagg gatggttgat gcgatttcca aatgttctgg
     4741 atttgtaaag cgtttgatgt agttgacatt gctgttaaag aaggcttcca agtcactatc
     4801 gctagtctta acaaggattg taataggttt gcttggtgct acatttactt ccgcacgcgc
     4861 attacgaacg gcacggatca agtctttgag actttctaca ccagtgtgag ccgcaaggtc
     4921 ttcaaaggct agattaacag ttgggtatgc agctgtcacg atagaacctt ctgagatttg
     4981 tccaaagatt tcctctgtca cgaatggcat gattgggtga aggagacgaa ggatcttgtc
     5041 cagagtgtaa aggagaacag atcgagtaat taccttatct tcttcattat cgctataaag
     5101 gacttccttg gtcaactcaa cataccagtc cgcaaattca tcccagatga agttgtagag
     5161 gatgtggcca gcaacaccaa actcaaactt atcaaagtta gcagttgctt ttccaatcgt
     5221 ttcattgagg ttgtggagaa tccagcggtc cgtcacatta ccagcctcac ctgttgcaac
     5281 ttttgtgaca ttgtcatgcg ccacatccag cgtcaaacct ccattgttca tgaggatata
     5341 gcgagaaatg ttccaaatct tgttaataaa gttccatgaa gcatccattt tctcataaga
     5401 gaaacgcacg tcttgtcctg gcgcagaacc gtttgaaagg aaccatcgaa gggcatcggc
     5461 accgtatttc tcgataacat ccattgggtc aatcccgtta ccgagagact tagacatctt
     5521 gcgtccttgc tcgtcacgaa taagaccgtg gataaggacg ttttggaatg gttgacggcc
     5581 tgtgaattcc aatgactgga agatcatacg agacacccag aagaagatga tgtcgtaacc
     5641 tgttaccaag gttgaagttg ggaaataacg tttaaagtct tctgagtcga cttcaggcca
     5701 gcccatggtt gaaaatggcc agagggcaga actgaaccaa gtatccaaga cgtcttcgtc
     5761 ctgagtccat ccgtcacctt ctggagcttc ttcgccgaca tacatttcac catcagcatt
     5821 gtaccaggca gggatttggt gaccccacca aagctgacga gagataaccc agtcgtggac
     5881 attttccatc cattgaagga aggtatcgtt gaaacgaggt gggtagaatt cgaccttgtc
     5941 ctctgtgtct tggttggcaa tggcgttctt agccaattgg tccatcttga cgaaccattg
     6001 agtagacaag cgtggctcaa ctacgacacc tgtacgttct gagtgaccaa cactgtggac
     6061 acgtttttcg attttgacaa gggcaccgat ttcttccaac ttagcaacga ctgccttacg
     6121 agcttcaaaa cgatccatgc ctgaaaattc aaaggcaagc tcattcatag ttccgtcgtc
     6181 gttcatgacg ttgacttgtg gcaagttatg acgttggcca accaagaagt catttggatc
     6241 gtgggcaggt gtgattttca cgacaccagt accaaactca ggatctgcgt gctcatctcc
     6301 aacgattggg atgagtttat tagcgattgg aaggatgacg tttttaccaa tcaagtcctt
     6361 gtagcgcggg tcttctggat taaccgcaac cgcaacgtcc ccaaacatag tctcaggacg
     6421 agttgtagca acttcaaggg cgcgtgaacc atcttccagc atgtaattca tgtggtagaa
     6481 ggcaccttcc acatccttgt gaatcacctc aatatcagaa agggctgtgc gagctgctgg
     6541 gtcccagttg atgataaact caccacggta gatccagcct ttcttgtaaa ggtccacaaa
     6601 gaccttacga acagcttttg acaaaccttc gtcaagagtg aaacgctcac gagaatagtc
     6661 tacagagagc cccatcttgc cccattgttc cttgatggta gtggcatatt cgtctttcca
     6721 ttcccagact ttcgtcaaga aagactcacg accaaggtca tagcgggaaa tgccctcacc
     6781 acgcaagcgc tcctcaacct tagcctgagt ggcaatccct gcgtggtcca tcccaggaag
     6841 ccaaagggta tcaaaacctt gcatgcgttt ttgacggatg ataatatctt gcaaagttgt
     6901 atcccaagcg tgaccaaggt gaagtttacc tgtaacgttt ggtggtggaa taacgattga
     6961 ataaggctta gccttttgat cgcctgaagg cttgaaaaca tcagcatcaa gccatttttg
     7021 gtaacgacca gcctcaacct cggctggatt gtatttagat gaaagttctt tagacatgtg
     7081 tgttctcctt aattactttt attgacaatt ttaaccaaac caatttttga aagttcaata
     7141 taaatctctt caatttcagc aaatgctgca agtggtgaca tagtataatt tccaggatta
     7201 tatttaagat catacttgct tatc
//
LOCUS       DAVLRT010000087         7108 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLRT010000087 DAVLRT010000000
VERSION     DAVLRT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.087"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(1..503)
                     /locus_tag="VXM85_001820"
                     /pseudo
     CDS             complement(1..503)
                     /locus_tag="VXM85_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(579..1019)
                     /locus_tag="VXM85_001821"
     CDS             complement(579..1019)
                     /locus_tag="VXM85_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1346348.1"
                     /translation="MKAPIPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLREKLLEQALLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQRRSGSVVQEFLGNYSGYMHCDMLRQ"
     gene            1010..1317
                     /locus_tag="VXM85_001822"
                     /pseudo
     CDS             1010..1317
                     /locus_tag="VXM85_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1340..1690)
                     /gene="tnpB"
                     /locus_tag="VXM85_001823"
     CDS             complement(1340..1690)
                     /gene="tnpB"
                     /locus_tag="VXM85_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1346349.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(1671..1856)
                     /locus_tag="VXM85_001824"
     CDS             complement(1671..1856)
                     /locus_tag="VXM85_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346350.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2160..3722)
                     /gene="guaA"
                     /locus_tag="VXM85_001825"
     CDS             complement(2160..3722)
                     /gene="guaA"
                     /locus_tag="VXM85_001825"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1346351.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3864..4562
                     /locus_tag="VXM85_001826"
     CDS             3864..4562
                     /locus_tag="VXM85_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1346352.1"
                     /translation="MLPAYMKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4605..5501)
                     /locus_tag="VXM85_001827"
     CDS             complement(4605..5501)
                     /locus_tag="VXM85_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1346353.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLSYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGILQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(5510..6445)
                     /locus_tag="VXM85_001828"
     CDS             complement(5510..6445)
                     /locus_tag="VXM85_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1346354.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQTFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6442..>7108)
                     /locus_tag="VXM85_001829"
     CDS             complement(6442..>7108)
                     /locus_tag="VXM85_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proteinase"
                     /protein_id="HEV1346355.1"
                     /translation="NETFYIETLGMKALLEESAFLSLGDQTGLEKLVLEEAPSMRTRK
                     VEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFEIFSPEDDLILIHAED
                     DRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDIESFLESSEIGASLDFIPAQG
                     QDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYFIPKSEKFFLGKDRNNVELWF
                     EEV"
BASE COUNT         2193 a         1539 c         1334 g         2042 t
ORIGIN      
        1 taatagctct tgctttagct ccttcaaaac tttgagaaaa aaccaccctc gcttttttaa
       61 actgagctta ggctaaaaga gtccagtgga ctcttttact ccgtcccata accaatgatt
      121 taatggcgcg ttcagctaga ttattggaaa ggaccagatg tccgtctttt aaaatggtct
      181 taaaggtttc ttcatacttg aggctgtatt caattgccct tcctggtttt gaacctgcta
      241 aaactgactg acggcggcac caagcaaaga agtcttccat taagggctgg acgtttctgt
      301 agtcgttcat cagctggcaa agcctcccag tctctttcca aggcaaataa ctggtcacga
      361 taggctaaac ctttagctcc taaggatgat ttatctgctt gcttgggggt cgcttcaaaa
      421 aatttccttc tcacatgcgc ccaacatcca acaagttcag cttcttccag ttgacgataa
      481 gcttctagcg gttcgcagtt taccaagctt agcgtcgcct tgctcctaaa ccttagactg
      541 ctatcgcata ggcagaacta gaggactaaa gttctaagtt actgccgcaa catatcacaa
      601 tgcatatagc cagaataatt tcctaggaat tcttgcacta ccgaaccact ccgacgctga
      661 tcatggtggt acagcgtaat cccttgtttc tcagctttcc cagacaaaaa agtccaatag
      721 taagtcagct gactatcact ttctaagacc cgataagagg tttcatctgc atgaagaaga
      781 gcttgttcta acaacttttc tcgtaaaagg ttataaaggg gctccaaata gtattgactc
      841 gtcttgatat gccaattaga gatttcctta cgtgtgattg gtaaacccat cctagcccaa
      901 tcttcttctt ggcgataatt gggtaccttc agattaaact tctgatggat ggtgtgagcg
      961 ataatagaag ctgagccaag gctatgcgcc aaaggggctt taggaatagg agctttcaca
     1021 agcttatcca gatgattatc ttttactcgt tatggacaat gctatatggc ataaatcaag
     1081 taccttaaag attccgacta atattggctt tgcatttatt cctccataca caccagagat
     1141 gaacccattg aacaagtgtg gaaagagatt cgtaaacgtg gatttaagaa taaagccttt
     1201 caaactttgg aagatgtcat gaatcaactt caagatgtta tacaaggact ggagaaggag
     1261 gtgataaagt ccatcgttaa tcggagatgg actagaatgc tttttgaaag cagatgagta
     1321 taaaaagaaa gtcctcattt caatagaaat cacgactttc tgataaattt attttgggag
     1381 tgatagaaaa gcccttcata agccaatcta cttgttcagg tgtgagagct ttgacatcct
     1441 tttctgtact gggccaagtc agtctgccgt tctcaaagcg tttatatagt agccaaaatc
     1501 cttgaccatc ccagtaaagg gctttaaagc ggtctttacg tccaccacaa aagagaaaaa
     1561 cttgaccgga gaaaggatcc aattcaaagt gggttttaac cagataagcc agtgaatcga
     1621 ttccttgtct catatcagtc ttcccacaca cgagatagac ctgccctagg ctagatagat
     1681 gaattgtcat agagcaacac cttatccaaa agctgttcta ccatttcgag attgagagat
     1741 tgaaaaaaac ttacttcaag cttgccaata cgaattttaa gcagaatatc atttctcttt
     1801 ttagaatcaa aaggacgaga ttgtggaatc tctacaggaa caataggttg tggcattaaa
     1861 atatcctcca atagttttac tttactaata gtatacagga ggaactccat gaatgataga
     1921 taccttgtta tgacgcgctt actatattag aaagttggct ctctaattaa attaattctc
     1981 attatatata tcaaaagacc gcagtttgaa aactacggtc taattttttg tccaaatgta
     2041 aatcacttgg aactattatt caatttattc tttttagtaa atgttcccgt tttaatccca
     2101 gttacagttt ttaatcctct gtaaccgttg attttacaaa gcttttaatt tttttgcgat
     2161 tactcccact caactgttgc aggtggttta cttgtaatat cgtagacgat acggttaaca
     2221 tgatccactt catttacgat acgtactgag attttttgaa gtacttccca tggaattttg
     2281 gcaaaatcag cagtcatacc atcgatagaa gtgatagcac ggattgcaat cgtgtagtca
     2341 tacgtacgac cgtcacccat aacaccgact gaacgaacgc ctgtgttaac agtgaagtat
     2401 tgccaaatat cgcggtcaag tccagcttta gcgatttctt cacgaagaat agcgtctgat
     2461 tcacgaacgg tttcaagttt ctcttcagtg atttcaccca tgacacggat agcaagtcct
     2521 ggtcctggga atggttggcg ccatacgata tggtctggca taccaagctc tgtaccaaga
     2581 gcacgaactt catccttgta aagagtattg agtggttcaa tcaattcaaa ctgcatatct
     2641 tctggaagac cacccacgtt gtggtgtgac ttgatagttt gagctgtatc cgtaccagac
     2701 tcgataacat ctgtatataa agtaccttga gcaaggaatt tcacatcttt gagcttgctt
     2761 gcttcgtcat cgaatacata gacaaactcg ttaccaatga ttttacgttt ttgttcagga
     2821 tcagaaacgc cagcaagttt gtcaaggaaa cgtttagcag cgtctgcttt gacaatattc
     2881 aaaccaaact taccaccgag catgtccata acttgatcag cttcgccttt acgaagaaga
     2941 ccgtggtcta cgaagataca gatcaattga tcgccaatcg ctttttggag aagaacccca
     3001 acgacagatg agtcaacacc acctgataga ccaagaagga cacgtttatc accgacggtt
     3061 tcacgaattt ttttgatctg catgtcaatg aaattatcca ttgaccagtc acctttagcc
     3121 ttacaaatgt taagggcaaa gttacgaagg atatcatttc cgtatacaga atgacgaact
     3181 tctgggtgga attggatacc gtaaatgtgt ttatctgggt tttcgatggc tgcgtatggg
     3241 cagtcagctg atgtacctgt acgaacaaag tcagcaggaa tctcagtaac cgcatcacca
     3301 tggctcatca aaacagtctg ttcatcaggt gttgattcaa aaagcgctga tggtgtgtga
     3361 gttagggttg attgaccgta ttcacgattt ccagcatcac ctgcaggaac aactttccct
     3421 ccaagtttat gggtcaataa ctgcatacca taacagattc ccaaaattgg aattccgagt
     3481 tcgaagattt ctgggtcaat atcaaatgaa ccatcttcat atacagaatt tggaccacct
     3541 gatagaataa ttcctacagg attgacttca cgaacttcag cagctgaaat tttatggctt
     3601 tttagttctg aaaaaacacc aatctcacgg atacggcgtg aaatcagctg gttgtactgg
     3661 ctaccatagt ccaatacgat gattttttct acatcttgca aatcagttga aatgttgctc
     3721 atctttttcc tttctcaaaa gaaatatctt ttcccaatat tctatcacaa ataagggcga
     3781 attaccaact aaacaaggcg aagtttgact ttttctagtt tattggggta caatagagaa
     3841 aagatagaaa tgaagtgaaa aatatgctac ctgcttatat gaaaatccat gatcagataa
     3901 aaaaggatat tgacgagtac cgttgggcta ttggtgagag gcttcccagt gaaagagatc
     3961 tagctgagca gtttgcggtc agtcgcatga ccctccgcca agccgtatct ctattagtcg
     4021 aagaaggcgt cttagagcgc cgtgtaggaa gcggcacctt tgtttccagt actcgagtac
     4081 aagaaaagat gcgagggaca accagtttta ctgaaattgt caaatcccaa ggtaaagttc
     4141 cctctagcca gctcatttcc tacaaaaaaa ccattcccaa tgagcaagaa gttgccaagc
     4201 taggaatttt tccaacggac aatattatcc gaatggagcg ggtccgctat gccgaccaag
     4261 ttcccctagt ttatgaagtt gcttctattc ctgaaaaatt cattaaggac tttaaaaaag
     4321 aagaaatcac cagtcatttc ttccaaacct tgcaaaaaca tggctatcgt atcggcaaat
     4381 ctcaacagac catctatgct cgccttgcta aagaaaagat tgcccactat ttggaagttg
     4441 aaaaaggaca tgctattctt ggattgaccc aggtttccta cctagaagat ggtactgctt
     4501 ttgaatacgt aaaaagtcaa tatgtaggcg aacgctttga attttatctt gaaaataatt
     4561 agattaccac gcaaaaactc tctgtcacgg acaaagagtc ttttttattt ttctaagaat
     4621 aaatctttca aagaaatcaa agttacagca aacaatatca ttgccatacc aataaaatca
     4681 atgggataaa attgttcatt cattattaag aaggcaaaga aaaccgccga tattggctca
     4741 attgaagcca acaagcttga cttgaccggt cctatcagac tggctccttt aaggaaagct
     4801 gtataggcaa agacagtccc gataaggata atgcctgcaa acgcaaggag aaaatcaaga
     4861 ctagtcggga tatcggcctg tagaatccct gtaaaaggaa gggcgaccaa acctgctatg
     4921 accattccca caccaatgac caagctgctc ccccactttt taatcaaggc tatgggtaaa
     4981 atgatataca aagcataagt caaggcagaa aagagacccc agaacagacc agcaggtgtc
     5041 atggataact ggtccaactg cccatgtgtt gcgatcagga aggttcctcc gatggcgaat
     5101 atgatggaaa ctatctctcc cagtgtcggt gccaccctat ccttgataca gctataaatt
     5161 aaaattccga caggacaaac atactgaagc accgtcgctg ttcccgcatt ggtctcctga
     5221 atagcagaca gataggcgaa ttggttgaga aaaagaccaa tcagagcaaa aataagaaga
     5281 gacagcaaac tctttctatc ctttaaaaag accagtattt tatcctttgc agtagcataa
     5341 gacaagagca tgagaattcc accagcgatt aaaagacgca agttggtcaa gaccagagcc
     5401 gaaattccgt gtgccattag gtattggcca ctcgttcctg acaaccccca agcaatacca
     5461 gccacaactg ttagtaaagt cccttttaaa ctatttgaca tgtctctcct tactcttctt
     5521 tgcgaatcaa gttcatcact tgattgcggt ctaatatcca ttgagctctc tcgtcctttt
     5581 cataggtacg attcgatagg aaaatagtgg cttcttgctt ctgacgattc cacatgataa
     5641 aggtacctgt atagcccgta tggtctagcc aatctccttc caaattccat gctaaagaac
     5701 gttccttgtc atccaaagga gaaaaatttt gatttaagtc tcttgcaaaa tcatctgcta
     5761 aatagtgttc taaaaaggtt tgtaaatcct ttatagtcga aaataaacca gcactcccag
     5821 catgtctacc caggagacga gccttgggat catgcactat gcctgcctct acacctctaa
     5881 ctgttggaac agcaagctca actggcccaa acttagtttc cgtcattccc caaggtttcc
     5941 agacttgatc ctttaaaatc acatccaaat cttgattaaa aattctttcc aaaataaagc
     6001 ccaacagcaa aaaatggaca tccgaataaa gaaaggctgg ctgacttcgt ctgttgagat
     6061 gaaacatcgc ttcctttaat tcaggggctg ttaaaagatc acgattagga ataaaaggat
     6121 caaggtctgt tgcatgagtc aagagctggc gaatagtgat gtctggataa tcactctcag
     6181 gtaaaaaatc tattaccagt ctatcaatat ctaattgacc tatttcccac aagaaggtac
     6241 aaactgtgcc aaccccaaca accttgctga cactagctag gtcataaact agtcctgcct
     6301 cagtctgcaa gccatgctct gggtcactct ggcctaaata gaactctgtc cattgattgt
     6361 ccttaaaata cgcaaaagag gctccgggat aaatccctgc ctcgatttgt tcttctattt
     6421 ttttaataat cttggtccac ttcatacttc ttcaaaccac aattcaacat tatttctatc
     6481 tttaccaagg aagaattttt cagacttagg aataaaatat tcagtagact caaacttctg
     6541 gcgaagactt gctatgtcta attcattgac caagaacttg agcatagata agtcccaggt
     6601 aaccgtattg tccacagtca aatcctgccc ctgagctgga ataaaatcaa gggatgcccc
     6661 aatttcagat gattccaaga aactttcgat atcagttggg agatgtaatt ccatagaaat
     6721 ctcaaattta cttaaagaaa ttgatgccaa atctgtttga aattcaggct tttctcctac
     6781 ttctactaga cttgctctgt catcttccgc atgaatcaaa atcaaatcat cttctggtga
     6841 gaaaatttca aaagcgtagc cattttgacc tttatataat cgatgaatcg aatctgtttt
     6901 agataagatt ccttcaattt ctaagggatt ttccaccttg acaatcaatc tagctagttt
     6961 ttttcttccc tctaccttac gagtacgcat actgggagct tcttctaaaa ccagcttttc
     7021 aagacccgtt tggtcaccta gtgacagaaa ggccgattct tctaacaagg ccttcattcc
     7081 aagggtttca atataaaatg tttcattt
//
LOCUS       DAVLRT010000088         7051 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLRT010000088 DAVLRT010000000
VERSION     DAVLRT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.088"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..413
                     /locus_tag="VXM85_001830"
     CDS             <1..413
                     /locus_tag="VXM85_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1346356.1"
                     /translation="EYGRSLKGQLIRGKVSGRRYQRISLVAGLTNGELIAPMTYEETM
                     TSDFFEAWFQNFLLPTLTTPSVIIMDNVRFHRMGKLELLCEEFGHKLLPLPPYSPEYN
                     PIEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN"
     gene            474..1436
                     /locus_tag="VXM85_001831"
     CDS             474..1436
                     /locus_tag="VXM85_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1346357.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYEDTSISVSSNETDKV
                     LAGSLYTDTNEQGLMIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAEASKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1613..2620
                     /gene="gap"
                     /locus_tag="VXM85_001832"
     CDS             1613..2620
                     /gene="gap"
                     /locus_tag="VXM85_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1346358.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2688..3524)
                     /locus_tag="VXM85_001833"
     CDS             complement(2688..3524)
                     /locus_tag="VXM85_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1346359.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3650..5845
                     /locus_tag="VXM85_001834"
     CDS             3650..5845
                     /locus_tag="VXM85_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1346360.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5898..6050
                     /gene="rpmG"
                     /locus_tag="VXM85_001835"
     CDS             5898..6050
                     /gene="rpmG"
                     /locus_tag="VXM85_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1346361.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6060..6236
                     /gene="secE"
                     /locus_tag="VXM85_001836"
     CDS             6060..6236
                     /gene="secE"
                     /locus_tag="VXM85_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1346362.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6291..6827
                     /gene="nusG"
                     /locus_tag="VXM85_001837"
     CDS             6291..6827
                     /gene="nusG"
                     /locus_tag="VXM85_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1346363.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2197 a         1222 c         1539 g         2093 t
ORIGIN      
        1 gagaatatgg tcgctcatta aaaggtcagt taataagagg caaagtatct ggaagaagat
       61 atcagaggat ttctttggtt gcaggtctaa caaatggtga attaatcgct ccaatgactt
      121 acgaagagac gatgacgagc gacttttttg aagcttggtt tcagaatttt ctcttaccaa
      181 cattaaccac accatcggtt attattatgg ataatgtaag attccataga atggggaagc
      241 tagaactttt atgcgaagag tttgggcata aactgttacc tcttcctccc tactcgcctg
      301 agtacaatcc tattgagaaa acatgggctc atatcaaaaa gcacctcaaa aaggtattac
      361 caagttgcaa taccttttac gaggctcttt tgtcttgttc ttgtttcaat tgactatagt
      421 tcacggatac agttgggaaa gaagctatta attatttgga gagtcctttt gggatgaaca
      481 tgtcaaatat tactatagat gggcaagaaa tggatctgag tcaaacgatg ccttcttatg
      541 ctcaagtagt gagtctttat gaggatacaa gcatctctgt tagtagtaat gagacggaca
      601 aagtgttggc tggatccctc tatactgata caaatgaaca gggattaatg attccaagca
      661 gtttactaaa aaattggaat gaacagacag gaaaaaattt gacagctaat gatcttattg
      721 gcaaatcagt ctcagccagc attgtagaaa gtgctgccga agctagtaag attgctcaat
      781 ttcaaacgaa gattgtacgt gtaatcaatg atgaagatga catggaggac agcaacagtt
      841 tcatgctgtc tcatcaaatg gaaacgattt tgaaagaggc tggatttacg aaagctgtat
      901 cttattttat cttggaactc aaagatccat cacagacaaa agtagtaaca gaagaattac
      961 agaaaaataa gaagtatact gtgctttctc aacagagagt tcttgatatt gtgattacct
     1021 ttattcgtgt tattcaggga ttattgattg tgctttcatc acaagctatt gtggtagcag
     1081 cggttatgat tggtatcatt atttacatca atatcatgca acgttccaag gaaataggtg
     1141 tcatgaaagc agttggttat cagaatcgtg gtgtcaaagg aatttttatt tacgaggcta
     1201 tctggattgt aggcatcgcc ttgctgctgg catttttggt tgcacaaggg gtgggaagtt
     1261 tggcgaatgc gattgtaagt cacttttacc catccatcac taaggttttt gaattaaatc
     1321 ttttatctgt tttaggaact ctagttttcg ctctattact tggttatgtc tcagcctact
     1381 tcccggcgcg taagattagt aaaatggatc ctgtagaatc gttacgctat gaataataag
     1441 ggattccttg atttacagtc ttaaaaagaa actcacatag aaaaacaatg gccatacttg
     1501 caattcaact caaatagttg tataataggt ttgttgaaag gtgatttgta gtgattcaag
     1561 ttactctttt cacaataaaa atttcatgat tttcataagg aggaaatcac taatggtagt
     1621 taaagttggt attaacggtt tcggacgtat cggtcgtctt gctttccgtc gtatccaaaa
     1681 cgtagaaggt gttgaagtta cacgcatcaa cgaccttaca gatccagtta tgcttgcaca
     1741 cttgttgaaa tacgacacaa ctcaaggtcg tttcgacggt actgttgaag ttaaagaagg
     1801 tggatttgaa gttaacggta aattcatcaa agtttctgct gaacgtgatc cagaacaaat
     1861 cgactgggct actgacggtg tagaaatcgt tcttgaagct actggtttct ttgctaagaa
     1921 agaagcagct gaaaaacacc ttaaaggtgg agctaaaaaa gttgttatca ctgctcctgg
     1981 tggaaacgac gttaaaacag ttgtattcaa cactaaccac gacgttcttg acggtactga
     2041 aacagttatc tcaggtgctt catgtactac aaactgcttg gctccaatgg ctaaagctct
     2101 tcaagacaac tttggtgttg ttgaaggatt gatgactact atccacgctt acactggtga
     2161 ccaaatgatc cttgacggac cacaccgtgg tggtgacctt cgccgtgctc gcgctggtgc
     2221 tgcaaacatc gttcctaact caactggtgc tgcaaaagct atcggtcttg taatcccaga
     2281 attgaatggt aaacttgatg gatctgcaca acgcgttcca actccaactg gatcagttac
     2341 tgaattggta gcagttcttg aaaagaacgt tactgttgat gaagtgaacg cagctatgaa
     2401 agcagcttca aacgaatcat acggttacac agaagatcca atcgtatctt cagatatcgt
     2461 aggtatgtct tacggttcat tatttgacgc aactcaaact aaagttcttg acgttgacgg
     2521 taaacaattg gttaaagttg tatcatggta cgacaacgaa atgtcataca ctgcacaact
     2581 tgttcgtact cttgaatact tcgcaaagat tgctaaataa ttcttgagtt gatagaaagc
     2641 aaggctttgt ggtcttgctt ttttatatgg aaaaatggat gacacgatca tccattcttt
     2701 tttaattctt tttcaaatgt atttgaaagg gtagtgaaag ttagcctctc taaagtaagt
     2761 gggtgggtaa aggaaagtcg gaaggcatga agcataagcc ggcttgtctt tgatttacta
     2821 ttatagagag ggtctcccag gataggaaga ttatgatgca aaaggtgcac acgaatctga
     2881 tgggttcgcc ctgtctttag cttgcaatga gccaaggaag tcttgtttga gaattgcttt
     2941 aatctgctta catgcgtttc agcatattgc ccattttttg catcaactat tctttttcta
     3001 cgatcatggc gatcacgtcc aattttgtct ctgaaaacaa gttcttttct gttgatattt
     3061 ccatcaacta gagcccaata ttctctagaa atctcttttt tctccaataa gcgattgaga
     3121 atgggcagga taaaaggatt tttggcaaag agaactaagc cactggtttc catgtccaga
     3181 cgatgaacga catagcaggt ttggccaaca taggtactga catggttaag aagggcaatt
     3241 tcgtttggtt gattaccatg cgttttcatc ccctctggtt tgtttacaat aatcaagtgt
     3301 tgatcttgat aaacttcctg cactaagtct gggttgcccc aagggatcgt cttttgggga
     3361 taatcttcct cgtcaaaagt caactggcaa acatctccag gatttacgat ttcgttccag
     3421 cggacttctt cttgatttat caaaatatgt ttcttgattc tcaaaaaatg acggattttt
     3481 ctagggatga ggagttgttc ctcaagtaat tgctttaccg tcatttgagg tagagaggcg
     3541 ggtaatgtaa atgtgaattg catacagata ttgtaacaaa aaaagcccta tttggatagg
     3601 aaatagctaa attcttgtct tcctatgatg aagatgataa aataaacgca tgaaattaga
     3661 taaattattt gagaaatttc tttctctttt taaaaaagaa acaagtgaac tagaggactc
     3721 tgattctact atcttacgtc gctctcgtag tgatcgaaaa aaattagccc aagtaggtcc
     3781 gattcgaaaa ttctggcgtc gttatcatct aacaaagatt atccttatac taggtttgag
     3841 tgcaggcttg ctagttggaa tctatttgtt tgctgtagcc aagtcgacca atgtcaatga
     3901 tttgcaaaat gccttgaaaa ctcggactct tatttttgac cgtgaagaaa aagaggctgg
     3961 tgccttgtct ggtcaaaagg gaacctatgt tgagctgact gacatcagta aaaacttgca
     4021 gaatgctgtt attgcgacag aagaccgttc tttctataaa aatgacggga ttaactatgg
     4081 ccgtttcttc ttggctattg tcactgctgg acgttcaggt ggtggctcta ccattaccca
     4141 acagctggct aaaaacgcct atttatcgca ggatcaaact gttgagagaa aagcgaaaga
     4201 atttttcctt gccttagaat taagcaaaaa atatagtaag gagcaaattc taaccatgta
     4261 ccttaacaac gcttattttg gaaatggtgt gtggggtgta gaagatgcga gtaagaaata
     4321 ctttggagtt tctgcatcag aagtgagtct ggatcaagct gcgactctgg cagggatgct
     4381 caaggggccg gaactgtata atcccttgaa ttccgtagaa gattctacta atcggcgcga
     4441 tactgtcttg cagaatatgg ttgcagcagg atatattgat aaaaaccaag aaaccgaagc
     4501 tgctgaagtt gatatgactt cgcaattgca cgataagtat gaaggaaaaa tctcagatta
     4561 ccgttacccc tcttattttg atgcggtggt taatgaagct gtttccaagt ataatctaac
     4621 agaggaagag attgtcaata atggctaccg catttacaca gagctggacc aaaactacca
     4681 agcaaatatg cagattgttt atgaaaacac atcgctattt ccgagggcag aggatggaac
     4741 gtttgctcaa tcaggaagtg tagctctcga accgaaaaca gggggagttc gtggagttgt
     4801 cggtcaagtt gctgacaatg ataaaactgg attccggaat ttcaactatg caacccaatc
     4861 aaagcgtagt cctggttcta caattaagcc tttagttgtt tatacgccag cagttgaagc
     4921 aggctgggct ttgaataagc agttggataa ccataccatg cagtatgaca gctataaggt
     4981 tgataactat gcagggatca aaacgagtcg agaagttcct atgtatcaag ccttggcaga
     5041 atcgcttaat ctacctgctg ttgccactgt taatgatttg ggtgtcgaca aggcttttga
     5101 ggcaggcgaa aaattcggac tcaacatgga aaaggtcgac cgtgttcttg gtgtcgcctt
     5161 gggaagcggt gttgaaacca accctcttca aatggctcaa gcatacgctg cctttgcaaa
     5221 tgaaggttta atgcctgaag ctcattttat tagtagaatt gaaaatgcta gtggacaagt
     5281 tattgcgaat cataaaaatt cacaaaaacg ggtgattgat aagtctgtag ctgacaagat
     5341 gaccagtatg atgttgggga ctttcaccaa cggtaccggt attagttcat cgcctgcaga
     5401 ctatgtcatg gcagggaaaa ctggaacaac tgaagcagtt ttcaatccgg agtacacaag
     5461 tgaccagtgg gtaattggtt atactccgga tgtagtgatt agccactggc ttggctttcc
     5521 gaccactgat gaaaatcact atctagctgg ctctacttca aacggtgcag ctcatgtctt
     5581 tagaaacatt gccaatacca ttttacctta tacgccagga agtaccttta cggttgaaaa
     5641 tgcttataag caaaatggaa ttgcaccagc caatacaaaa agacaagtac aaaccaatga
     5701 taatagccag acagatgata atttgtctga tattcgaggg cgtgcgcaaa gtctagtaga
     5761 tgaggctagc cgggctatct cagatgcgaa gattaaggaa aaggctcaaa caatatggga
     5821 ttcgatagtc aatctatttc gctaagatgc ttgtcaaagc ctagctttct tgttataatg
     5881 gataagatgg aggcgttatg gcactaaaaa aagcaagcct agcttgtgcg gtttgtggtt
     5941 cgagaaacta ttcaatcaag atcagcggaa accccaagcc tacacgacta gaagtaaata
     6001 aattttgtaa gcattgtggc aagtacacta cacacagaga aacgagatag gagagagcga
     6061 tgcgttttat tggagatatt tttagacttc ttaaagacac aacatggcca actcgcaagg
     6121 aaagctggag agattttcgt tctatcatgg aatacacagc tttctttgta gtaattattt
     6181 acatttttga ccagttgatt gtttcaggtt tgattcgatt tattaacatt ttttagaaga
     6241 ttagtggagt taattacact agaaatcttc tatttatgaa aggaaatatc atggatagtt
     6301 ttgataaagg atggtttgtt ttacaaactt attctggtta tgaaaataag gtgaaagaaa
     6361 atctattaca acgtgcacaa acctacaata tgttggataa tattctacgc gttgaaattc
     6421 caacacaaac agtgcaagtt gaaaaaaatg gaaagagaaa agaagtagaa gaaaatcgct
     6481 ttccaggtta tgttcttgta gaaatggtca tgacagatga agcttggttt gttgttcgaa
     6541 acacaccaaa tgttacagga tttgtcggat cacacgggaa cagatcaaaa ccaactccat
     6601 tattggaaca agaaattcgt gacattttgg tatctatggg acaaactgtt caagaatttg
     6661 atttcgatgt tgagattggt caaactgtac gtattattga tggtgctttt gcagactaca
     6721 ctggtaagat tacagaaatt gataataaca aagtgaaaat gattatctct atgtttggta
     6781 atgacacagt tgcagaagta aacctaaacc aaatcgcaga attataaccc tgagagaggc
     6841 tggagcctct cttttttgtg cagtttagga gctaaaggga acagaatgga gaaaatggaa
     6901 caaatgtgtt ttctaatctg ttagactgta tctagaaagg ggaaaattat gattaaagaa
     6961 ttgtatgaag aagtccaagg gactgtgtat aagtgtagaa atgaatatta ccttcattta
     7021 tgggaattat cggattggga ccaagaaggc a
//
LOCUS       DAVLRT010000089         6957 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLRT010000089 DAVLRT010000000
VERSION     DAVLRT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.089"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            252..2585
                     /locus_tag="VXM85_001838"
     CDS             252..2585
                     /locus_tag="VXM85_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1346364.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2598..4061
                     /locus_tag="VXM85_001839"
     CDS             2598..4061
                     /locus_tag="VXM85_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1346365.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4061..5629
                     /locus_tag="VXM85_001840"
     CDS             4061..5629
                     /locus_tag="VXM85_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1346366.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            5686..6483
                     /gene="psrA"
                     /locus_tag="VXM85_001841"
     CDS             5686..6483
                     /gene="psrA"
                     /locus_tag="VXM85_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1346367.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            6494..>6957
                     /locus_tag="VXM85_001842"
     CDS             6494..>6957
                     /locus_tag="VXM85_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1346368.1"
                     /translation="MVKLTTCYFCDYGSYYGNKDETTSYPIYEIPEAWRYIKFASLVN
                     FRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTL
                     LMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLG"
BASE COUNT         2456 a         1031 c         1360 g         2110 t
ORIGIN      
        1 ttatttattt ttgaaaaata gctcaccagc ccgactgaaa gtgcttataa atgataataa
       61 gtcgcctgcc gaaaacagcg aaaaatagcg gtgttatgcg gagataatct gacgcgatgc
      121 gaaagtatat tgcatactta ttttcaacaa tttagcagag tatttttata agtgtgatat
      181 aatagaagta taatttgttc tgatagttta ttttatggag aagtagattt ttagaatgcg
      241 gagggttcaa tatggttgag tttataaagt ctaagaaaga aatgagtgag gaggatatta
      301 aagcaaattt catcactcct gctattgtat ccaaaggatg gaaaaatggt gagcatatcg
      361 cttacgaaga atacttcact gatggtcgaa ttgaggttag aggagataag gctcgtcgta
      421 aagaaggaaa aaaatcagac tattcactgt attaccaatt tggaactcga attgcaattg
      481 ttgaggcaaa ggataataaa cacagcgttc gagcaggatt acaacaagct attgaatatg
      541 gagagatttt agatgttcca tttgtttatt cttcgaatgg tgatggcttt attgaacacg
      601 accgtatcac gagagaagaa cgtgagctgg agttagacga attccctact cgtgaagaat
      661 tattttctcg tatgacgaag gaaaaaggat tgacgtacga aattacagaa gctatctcaa
      721 ctccatacta tacagacgcc ttctcaatga aaacgccacg ctattatcag caaatagcta
      781 tcaaccgtac tattgaaaca gttgccagag gacaaaaacg agtaatgttt gtgatggcaa
      841 caggaacggg gaaaacgttc atggcttttc aaattattca tcgccttcga aaagctggtt
      901 tggctaaacg agttttattc ttagcggata gaaacatctt agtagaccaa acgatggctg
      961 aagactttag gccattcgaa aaggtaatga cgaaaattac accaaaactt ttgactgctc
     1021 ctgaaaaatt aaattctttt gaaatttatc tagggcttta tcagcaacta actggtgaag
     1081 atggaactga aacacattat caaaaatttg acaaagactt ctttgattta atcgtaattg
     1141 atgaagcgca ccgtggttca gctaaggaaa acagtaactg gcgtaagata attgattatt
     1201 tcagttctgc gacacagatt gggatgaccg ctactcctaa agaaaccaag aatgcttcca
     1261 atacggaata ctttggtgag ccaatctata cttatagttt aaaacaggga atcgaggatg
     1321 gttttttggc tccatatcgt gttatgagag ttaatttaga tgtagatgtg gatggttatc
     1381 gtccagaaac tggaaaagtt gatgctaacg gacaattaat agaagatagg tactacggca
     1441 ggaaagattt tgataaaacc attgtcattg atgatagaac gcaaagagtt gccaagtttg
     1501 tttctgatta tatgaagcaa aacaatgcac gatttgataa aacaattgtt ttttgtgttg
     1561 atattgacca tgccgagcga atgcgtgctg cacttgtaaa agagaatcta gacttagtcc
     1621 aagaagacta tcgttatgtc atgcaagtaa ctggtgacaa cgctgaagga aaagctcaac
     1681 tggataactt tatggatgtc aattctaatt ttcccgctat tgtaacaacg tctaaattat
     1741 taacgacagg agttaatgct aaaacatgtc gtttgattgt tttagactct aatatccaat
     1801 ccatgactga atttaaacaa attattggtc gtggcacacg tctttatcct caaaagggga
     1861 aagaattttt tacgattatt gattttcgaa atgttaccaa tttgtttgct gaccctgatt
     1921 ttgatggtga tccagtgaag gtgctggaaa caggtgcgaa aacagtcagt ggttctacgc
     1981 ccggtttcgt aagtgaggaa ggtgacccag tagaaaaata tatcgttaca gacaagcagg
     2041 ttaccattct taattctact gttcaagtat tggatgaaaa cgggaaactg attaccgaaa
     2101 gcctgaccga ctacactcgg aagaatatct taggtagcta cgccactttg aacgatttta
     2161 tcacagtttg gcatacggcg gataaaaaga agcttatctt agacgaactt tataaaaaag
     2221 gagtttatct agatgctatt cgagagtcgg agggaatatc agaacaagaa atcgatgatt
     2281 ttgatttact cctaaaactt gcctatggtc aaaaagaatt aaccaaaacg gaacgtatca
     2341 ataagctcaa acaaagcgga tatttatata aatatagtga ggaagcgcgt gctgttttgg
     2401 aaattttact gaacaaatac atggataaag gtattggaga actcgaaagc attgaaacat
     2461 taaaacttcc agaatttcag atatatggtg gaaccttcaa aatcatcaat acttattttg
     2521 gagataaaaa acgatattta caagcaatta aagaattgga gcaagagcta tttacagtag
     2581 cttaatgaaa ggaaagtatg tcaattacat catttgtaaa aagaattcaa gatatcactc
     2641 gaaacgatgc tggtgttaat ggtgatgctc aacgtattga gcaaatgtct tggttattat
     2701 tcttaaaaat ttatgatagc cgtgaaatgg tttgggaatt agaagaagac gagtatgagt
     2761 caattatccc agaggaatta aaatggcgaa attgggctca tgctcaaaat ggggaacggg
     2821 tattgacagg cgatgaatta cttgattttg tcaataacaa gttattcaaa gagttgaaag
     2881 agcttgaaat aacttcaaat atgcctattc gaaaaacgat tgttaaatca gcttttgaag
     2941 atgcgaacaa ctatatgaaa aatggcgtct tgttacgcca agtcatcaat gttattgatg
     3001 aagttgattt caatagccct gaagatcgtc attcgtttaa tgatatttac gaaaaaattc
     3061 ttaaagatat tcaaaatgct gggaactcag gagaatttta tacgccacgt gcagcgactg
     3121 attttattgc cgaagttctt aacccaaaac ttggagaatc aatggcagac cttgcttgcg
     3181 gaacaggagg cttcttgact tcgactctga accgtttaag tagtcaacgt aaaactagtg
     3241 aagataccaa aaaatataat acagctgttt ttggtattga aaagaaagca tttcctcatc
     3301 ttttagcagt tacaaatctg tttcttcacg aaattgatga ccctaaaatt gttcatggaa
     3361 atactttaga gaaaaatgtt cgtgaatata cggatgatga aaaatttgac attattatga
     3421 tgaatccacc ttttggaggg tcagaattag aaacaataaa aaataacttt ccagcagaat
     3481 tacggagttc tgaaacagct gatttattta tggctgtcat tatgtatcgt ttgaaagaaa
     3541 atggtcgtgt tggagttatt ttacctgatg gttttctatt tggtgaaggt gtaaaaactc
     3601 gcttgaaaca aaaactggta gatgagttca acttgcatac gattattagg ttgcctcata
     3661 gtgtctttgc accgtataca ggaatccata cgaacattct tttctttgat aaaacaaaga
     3721 aaacagaaga aacttggttt tatcgtttag atatgccaga tggttataaa aatttctcga
     3781 aaactaagcc gatgaagtca gaacacttca atcctgttcg tgactggtgg gaaaatcgtg
     3841 aagagattct ggaaggtaag ttctacaaat ctaaatcatt tacacctagt gaattggctg
     3901 agttgaatta taatttagac cagtgtggtt ttccaaaaga ggaagaggaa atcttaaatc
     3961 cctttgagtt gattcagaat tatcaagcgg aaagagcaac tttaaatcat aagattgata
     4021 atgtattagc tgatattttg cagttgttgg aggacaaata atgacaccag aacaacttaa
     4081 ggcaagtatt ctccaaagag cgatggaagg gaaattagtg ccgcaaaatc ccaatgacga
     4141 acctgcaagt gaattattaa agagaattaa agctgaaaaa gaaaaactta tcagtgaagg
     4201 aaaaatcaaa cgagataaaa aggaaactga gatatttcgt ggtgatgatg ggaaacatta
     4261 tgggaagttt gctgatggaa gcactcaaga aattgatgtt ccttatgata ttcctgatac
     4321 ttgggagtgg gtgaggtttt ctacattggt tgaaattgtc agaggtggct ctccacgacc
     4381 aatcaaagat tatcttactt ctgaagtaga tggaataaat tggataaaaa taggtgatac
     4441 tgaaaagggt gaaaagtata taaataatgt taaagaaaaa atcaaaaaat cagggcttaa
     4501 caaaactaga tttgtaaaaa aaggtacatt tttgttaact aattctatga gttttggtag
     4561 accttatatt ttgaatgttg atggtgcaat acacgatgga tggttggcta tttcgaacta
     4621 tgaaaactca ttaaataaag attacctatt ctatattctt tcatcaaatg tagtttattc
     4681 tcaatttcta tctctaatta gtggagctgt tgtgaaaaac ttgaatagtg ataaagttgc
     4741 ttctattctt atccctctcc ccccactatc cgaacaacaa cgcataatag aagcaatcga
     4801 atcagcttta gaaaaagtag atgaatatgc tgaaagttat aatagactag aacagctaga
     4861 taaagaattt ccagataaac taaaaaaatc tattcttcaa tatgctatgc aaggaaaatt
     4921 agttgaacaa gacccaaatg atgaatcagt cgaagtttta cttgaaaaaa tacgagcaga
     4981 aaaacaaaaa ctctttgaag aaggcaagat taaaaagaaa gatttggaca tttctattgt
     5041 ttcccaagga gatgataact cttattatga ggaagtacct tgtgaaatac cagaaagttg
     5101 ggaatgggtg aggttaaacg atataacctc ctacattcaa agagggaagt ctccaaaata
     5161 ttcaaatatt cctatatacc ctgtgattgc tcagaaatgt aatcaatggt ctggcttttc
     5221 tattgactta gcaagattta ttgatcccga aacagttcat agttaccaga aagaaagatt
     5281 actaagagat ggtgatttaa tgtggaattc aacaggtcta ggtacgttag gacgattagc
     5341 tatttaccat gaaaataaaa atccttatgg ctgggctgtt gcagatagcc atgttacagt
     5401 tataagagtc ttatcgggag ttattaattg ccatttcatc tataattttc tttcctctcc
     5461 tattgttcag tcagttattg aagaaaaagc gtcgggtagt acaaaacaaa aagaattatt
     5521 aacgaaaact ataaaagaat atttaatccc cctcccaccc cttcctgaac aatccagaat
     5581 cgttgataaa atcgaacaat ttttcgccca tattgacgca ctaatttagt ataatagttt
     5641 tacgtgcggt ggaatttcta tcaaacataa gataggagtt ttacgttgaa agattttgaa
     5701 ttacatttga agaaagctgg tttagctgaa aatactgtgc gttcgtacct ttatggtgtt
     5761 cgatttttct tggagaatta cgaactcaaa atggaagatt tatttgaata caaaggatat
     5821 ttattagaca attttaaacc taaaactgtc aatttgcgac tacaaggagt aaataaatat
     5881 ctggctttta ttggtcatga tgatttgaaa ttaaaatttg tcaaagtaca gcaaaaaccg
     5941 tttttagaag atgtcattag ccatgctgat tacctttttc ttaaacgtag tttgaaaaaa
     6001 gatggtattt taaaatggca ttttgtcgtt tggtttttag gtgcaactgg ggctcgtgta
     6061 agtgaactta ttaaactaaa agtagagcat gtagaaatcg gttattttga catttactca
     6121 aaaggtggta aaattcgtag actgtacatt cctaaaaaat taagaaatag ttgtcttagt
     6181 tggttagaat cagaaaatcg ccgaagtggt tatttatttt tgaataaatt taacgaacca
     6241 attacagcaa gaggagttgc tcaacagtta aaaaattatg ctgataaata caaaatgaat
     6301 cctaaagtaa tttaccctca ttcttttagg catttatttg ctaagaattt tttagcgaag
     6361 tataatgata ttgccttgct tgcagatttg atgggacacg aaagtataga aactactcga
     6421 atttatctaa ggaaaacagc tactgaacaa caaaatattg tagataaaat tgttaattgg
     6481 taaaaaataa caggtggtca aactgactac ctgctatttt tgtgattatg gctcttatta
     6541 tgggaataaa gacgaaacaa cctcttatcc tatatatgaa atcccagaag catggagata
     6601 tattaaattt gctagcctag ttaacttcag aataggaaaa actcctccac gcagtgaagc
     6661 tactttttgg ggaactgaaa taccttgggt atctatatca gatatgccta tatctggtta
     6721 tgtaactaat acgagagaat ctatttctaa attagcatta aaatcaaaga aaatagacat
     6781 ttcacctaaa ggaactttat taatgagttt taaattatct attggaaaag ttgccatatt
     6841 ggatattcct gccactcata atgaagccat tatatcgata ttcccctatg ctaataaaga
     6901 aaatattatc agagattact taatgatatt tttgccactt atctctactt taggtga
//
LOCUS       DAVLRT010000090         6929 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLRT010000090 DAVLRT010000000
VERSION     DAVLRT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.090"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            84..6518
                     /locus_tag="VXM85_001843"
     CDS             84..6518
                     /locus_tag="VXM85_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1346369.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKE
                     ENSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKGLSNLKNTKVLYT
                     YDRIFNGSAIETTPDNLDKIKQIEGISSIERAQKVQPMMNHARKEIGVEEAIDYLKSI
                     NAPFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDK
                     IPHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNA
                     QIFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRA
                     LRKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQ
                     TVEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLD
                     LRGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEG
                     TKSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNV
                     GDEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVI
                     NGKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIAL
                     QNTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYG
                     SISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDG
                     KQIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFES
                     VEEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDG
                     KPKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSS
                     GSKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVI
                     SREHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPAT
                     KIRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLI
                     TKDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTY
                     AGEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFADQADEK
                     GMISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEG
                     EASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKRNIIA
                     YDDGSALQYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT         2742 a          941 c         1343 g         1903 t
ORIGIN      
        1 ttagtaaaac tttttataac aaaggctagc aaagttaaag tttattatct attggaagtt
       61 aaataaatat gtaaggaatt aaaatgaaaa aaagcacagt attgtcatta actacagctg
      121 cagttatttt agcagcctat gcccctaatg aggtagtctt agcagacaca tctagctctg
      181 aagatgcttt aagcatctct gataaagaaa aagtagtagt agataaggaa acagaaaata
      241 aagagaaaca taaagatatt cataatgcta tagaaacttc aaaggatact gaagaaaaga
      301 aaacaacaat tattgaggaa aaagaagttg ttagtaaaaa tcctgtaata gacactaaaa
      361 ctagcaatga agaagcaaaa atcaaagaag aaaattccaa tcaatcccaa ggagatcata
      421 cggactcatt tgtgaataaa aacacagaaa atcccaaaaa agaagataaa gttgtctata
      481 ttgctgaatt taaagataaa gaatctggag aaaaagcaat caagggacta tcaaatctta
      541 agaatacaaa agttttatat acttatgata gaatttttaa cggtagtgcc atagaaacaa
      601 ctccagataa cttggacaaa attaaacaaa tagaaggtat ttcatcgatt gaaagggcac
      661 aaaaagtcca acccatgatg aatcatgcca gaaaggaaat tggagttgag gaagctattg
      721 attacctaaa gtctatcaat gctccgtttg ggaaaaattt tgatggtaga ggtatggtca
      781 tttcaaatat cgatactgga acagattata gacataaggc tatgagaatc gatgatgatg
      841 ccaaagcctc aatgagattt aaaaaagaag acttaaaagg aactgataaa aattattggt
      901 tgagtgataa aatccctcat gcgttcaatt attataatgg tggcaaaatc actgtagaaa
      961 aatatgatga tggaagggat tattttgacc cacatgggat gcatattgca gggattcttg
     1021 ctggaaatga tactgaacaa gatatcaaaa actttaacgg catagatgga attgcaccta
     1081 atgcacaaat tttctcttac aaaatgtatt ctgacgcagg atctgggttt gcgggtgatg
     1141 aaacaatgtt tcatgctatt gaagattcta tcaaacacaa cgttgatgtt gtttcggtat
     1201 catctggttt tacaggaaca ggtcttgtag gtgagaaata ttggcaagct attcgggcat
     1261 taagaaaagc aggcattcca atggttgtcg ctacgggtaa ctatgcgact tctgcttcaa
     1321 gttcttcatg ggatttagta gcaaataatc atctgaaaat gaccgacact ggaaatgtaa
     1381 cacgaactgc agcacatgag gatgcgatag cggtcgcttc tgctaaaaat caaacagttg
     1441 agtttgataa agttaacata ggtggagaaa gttttaaata cagaaatata ggggcctttt
     1501 tcgataagaa taaaatcaca acaaatgaag atggaacaaa agctcctagt aaattaaaat
     1561 ttgtatatat aggcaagggg caagaccaag atttgatagg tttggatctt aggggcaaaa
     1621 ttgcagtaat ggatagaatt tatacaaagg atttaaaaaa tgcttttaaa aaagctatgg
     1681 ataagggtgc acgcgccatt atggttgtaa atactgtaaa ttactacaat agagataatt
     1741 ggacagagct tccagctatg ggatatgaag cggatgaagg tactaaaagt caagtgtttt
     1801 caatttcagg agatgatggt gtaaagctat ggaacatgat taatcctgat aaaaaaactg
     1861 aagtcaaaag aaataataaa gaagatttta aagataaatt ggagcaatac tatccaattg
     1921 atatggaaag ttttaattcc aacaaaccga atgtaggtga cgaaaaagag attgacttta
     1981 agtttgcacc tgacacagac aaagaactct ataaagaaga tatcatcgtt ccagcaggat
     2041 ccacatcttg ggggccaaga atagatttac ttttaaaacc cgatgtttca gcacctggta
     2101 aaaatattaa atctacgctt aatgttatta atggcaaatc aacttatggc tatatgtcag
     2161 gaactagtat ggcgactcca atcgtggcag cttctactgt tttgattaga ccgaaattaa
     2221 aggaaatgct tgaaagacct gtattgaaaa atcttaaggg agatgacaaa atagatctta
     2281 caagtcttac aaaaattgcc ctacaaaata ctgctcgacc tatgatggat gcaacttctt
     2341 ggaaagaaaa aagtcaatac tttgcatcac ctagacaaca gggagcaggc ctaattaatg
     2401 tggccaatgc tttgagaaat gaagttgtag caactttcaa aaacactgat tctaaaggtt
     2461 tggtaaactc atatggttcc atttctctta aagaaataaa aggtgataaa aaatacttta
     2521 caatcaagct tcacaataca tcaaacagac ctttgacttt taaagtttca gcatcagcga
     2581 taactacaga ttctctaact gacagattaa aacttgatga aacatataaa gatgaaaaat
     2641 ctccagatgg taagcaaatt gttccagaaa ttcacccaga aaaagtcaaa ggagcaaata
     2701 tcacatttga gcatgatact ttcactatag gcgcaaattc tagctttgat ttgaatgcgg
     2761 ttataaatgt tggagaggcc aaaaacaaaa ataaatttgt agaatcattt attcattttg
     2821 agtcagtgga agaaatggaa gctctaaact ccaacgggaa gaaaataaac ttccaacctt
     2881 ctttgtcgat gcctctaatg ggatttgctg ggaattggaa ccacgaacca atccttgata
     2941 aatgggcttg ggaagaaggg tcaagatcaa aaacactggg aggttatgat gatgatggta
     3001 aaccgaaaat tccaggaacc ttaaataagg gaattggtgg agaacatggt atagataaat
     3061 ttaatccagc aggagttata caaaatagaa aagataaaaa tacaacatcc ctggatcaaa
     3121 atccagaatt atttgctttc aataaccaag ggatcaacgc tccatcatca agtggttcta
     3181 agattgctaa catttatcct ttagattcaa atggaaatcc tcaagatgct caacttgaaa
     3241 gaggattaac accttctcca cttgtattaa gaagtgcaga agaaggattg atttcaatag
     3301 taaatacaaa taaagaggga gaaaatcaaa gagacttaaa agtcatttcg agagaacact
     3361 ttattagagg aattttaaat tctaaaagca atgatgcaaa gggaatcaaa tcatctaaac
     3421 taaaagtttg gggtgacttg aagtgggatg gactcatcta taatcctaga ggtagagaag
     3481 aaaatgcacc agaaagtaag gataatcaag atcctgctac taagataaga ggtcaatttg
     3541 aaccgattgc ggaaggtcaa tatttctata aatttaaata tagattaact aaagattacc
     3601 catggcaggt ttcctatatt cctgtaaaaa ttgataacac cgcccctaag attgtttcgg
     3661 ttgatttttc aaatcctgaa aaaattaagt tgattacaaa ggatacttat cataaggtaa
     3721 aagatcagta taagaatgaa acgctatttg cgagagatca aaaagaacat cctgaaaaat
     3781 ttgacgagat tgcgaacgaa gtttggtatg ctggcgccgc tcttgttaat gaagatggag
     3841 aggttgaaaa aaatcttgaa gtaacttacg caggtgaggg tcaaggaaga aatagaaaac
     3901 ttgacaaaga cggaaatacc atttatgaaa ttaaaggtgc aggagattta agaggaaaaa
     3961 tcattgaagt cattgcatta gatggttcta gcaatttcac aaagattcat agaattaaat
     4021 ttgctgatca ggctgatgaa aaggggatga tttcttatta cctagtagat cctgacaaag
     4081 atgcttctaa atatgaaaaa cttggtgaaa tttctgaaga taaactcaaa aatgcaaaga
     4141 gcccagagga aaataccaat aataatcaag ctaaggatga agattcaaaa ccagatgaaa
     4201 aaagttcagt tgagggagaa gctagccttg aaataaataa aactatttca acaattagag
     4261 agtttgaaaa taaagaccta aagaaactca ttaaaaagaa atttagagaa gttgatgatt
     4321 ttacaagtga aactggtaag agaatagagg aatacgatta taaatacgat gataagagaa
     4381 atattattgc ttatgacgat ggtagtgcct tacaatatga aactgaaaaa tttgacgaaa
     4441 taaaatcaaa aatttatggt gttctaagcc catctaaaga tggacacttt gaaattcttg
     4501 gaaagataag caatgtttct aaaaatgcca aggtatatta tggaaatagc tataaatcga
     4561 tagaaatcaa agcgactaag tatgattcac attcaaaaac gatgatattt gatttatacg
     4621 ctaatattaa tgatattgta gatggattag cttttgctgg agatatgaga ttatttgtta
     4681 aagatgacaa tcaaataaaa gctgaaacta agattagaat gcctgaaaaa aataaggaaa
     4741 ctaaagcaga atatccctat gtatcaagtt atgggaatgt aatcgaatta ggagaaggag
     4801 atctttcaaa gaacaaacca gacaatttaa ctaaaatgga atctggtaaa atctattctg
     4861 attcagaaaa acaacaatat ctgttaaagg ataacatcat tctaagaaaa ggctatgcac
     4921 taaaagtgac tacctataat cctggaaaaa cggatatgtt agaaggaaat ggagtctata
     4981 gcaaggaaga tatagcaaaa atacaaaagg ccaatcctaa tctaagagtc ctttcagaaa
     5041 caacaattta tgctgatagt agaaatgttg aagatggaag aagtacccaa gctgtattaa
     5101 tgtcggcttt ggacggcttt aacattataa ggtatcaagt gtttacattt aaaatgaacg
     5161 ataaagggga agctatcgat aaagacggaa atcttgtgac agattcttct aaacttgtat
     5221 tatttggtaa ggatgataaa gaatacactg gagaggataa gtccaatgta gaagctataa
     5281 aagaagatgg ctccatgtta tttattgata ccaaaccagt aaacctttca atggataaga
     5341 actactttaa tccatctaaa tctaataaaa tttatgtacg aaatccagaa ttttatttaa
     5401 gaggtaagat ttctgataag ggtggtttta actgggaatt gagagttaat gaatcggttg
     5461 tagataatta tttaatctac ggagatttac acattgataa cactagagat tttaatatta
     5521 agctgaatgt taaagacggt gacatcatgg actggggaat gaaagactat aaagcaaacg
     5581 gatttccaga taaggtaaca gatatggatg gaaatgttta tcttcaaact ggctatagcg
     5641 atttgaatgc taaagcagtt ggagtccact atcagttttt atatgataat gttaaacctg
     5701 aagtaaacat tgatcctaag ggaaatacta gtatcgaata tgctgatgga aaatctgtag
     5761 tctttaacat caatgataaa agaaataatg gattcgatgg tgagattcaa gaacaacata
     5821 tttatgtaaa tggaaaagaa tatacatcat ttgatgatat taaacaaata acagacaaga
     5881 cactaaacat taagattgtt gtaaaagatt ttgcaagaaa tacaaccgta aaagaattca
     5941 ttttaaataa agatacggga gaggtaagtg aattaaaacc tcatagggta actgtgacca
     6001 ttcaaaatgg aaaagaaatg agttcaacga tagtgtcgga agaagatttt attttacctg
     6061 tttataaggg tgaattagaa aaaggatacc aatttgatgg ttgggaaatt tctggtttcg
     6121 aaggtaaaaa agacgctggc tatgttatta atctatcaaa agataccttt ataaaacctg
     6181 tattcaagaa aatagaggag aaaaaggagg aagagaataa acctactttt gatgtatcga
     6241 aaaagaaaga taacccacaa gtaaatcata gtcaattaaa tgaaagtcac agaaaagagg
     6301 atttacaaag agaagatcat tcacaaaaat ctgattcaac taaggatgtt acagctacag
     6361 ttcttgataa aaacaatatc agtagtaaat caactactaa caatcctaat aagttgccaa
     6421 aaactggaac agcaagcgga gcccagacac tattagctgc cggaataatg tttatagtag
     6481 gaatttttct tggattgaag aaaaaaaatc aagattaaga caaaagctat agaaaaaaat
     6541 ggtttatgta ctgagattag atagtgaggt gatgacatag ttttgtgaaa acagccattt
     6601 ataactcaat tatttagttt acttcactat tgatactatt tggagttatt aatggactta
     6661 gtttagataa ctaatgaatt gattgaaagg gttagtattg acaatattgg tcatattgat
     6721 tagaaaatag agtctatcaa aatttaaagg ctaatagagg tgatgagaca atttcaagga
     6781 attaagtagc gtttggttgc ttaattcctt tttatttgga aggaagtttt tcgtaaccgc
     6841 ccaataacga agtctattgg acggttacga tatgatcact gacttcgtca gtcttatcta
     6901 caacctcaaa actgtgtttt gagcaacct
//
LOCUS       DAVLRT010000091         6907 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLRT010000091 DAVLRT010000000
VERSION     DAVLRT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.091"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(95..1645)
                     /locus_tag="VXM85_001844"
     CDS             complement(95..1645)
                     /locus_tag="VXM85_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1346370.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(1661..2440)
                     /locus_tag="VXM85_001845"
     CDS             complement(1661..2440)
                     /locus_tag="VXM85_001845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1346371.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(2427..3278)
                     /gene="ylqF"
                     /locus_tag="VXM85_001846"
     CDS             complement(2427..3278)
                     /gene="ylqF"
                     /locus_tag="VXM85_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1346372.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEKAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(3359..4867)
                     /locus_tag="VXM85_001847"
     CDS             complement(3359..4867)
                     /locus_tag="VXM85_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1346373.1"
                     /translation="MYYVTKTNSKGQPLYQVVEKYKDPLTDKWRTVTVSYTRNTSRAR
                     KQAEREVMDKIEKLTSSLESQYNPEMITTFGELKESWFQTWCVSVKPQTIKREELVIE
                     RLGKIIGDEFLLDRITPLLMKNCLNKYLDKYDASPSTMTHIKSTCNKIFNHGVLYNVI
                     KYSPMTAVKIDVPLEKKRESRKRLDAKFLAIHELQAFFDVLGQCRNASYYDLAIVLLL
                     TGIRIGEAAFLPSDVDFDKGILHIDKALQYHDLKVDEFYFDTTKTVNADREVALPIAA
                     SEAIQRTIKRNQEFEAYMEQNPSPAFKKCDSVFQTEYGSPITSHSFRQVLKRVEEKLL
                     KNCQKDYGFKWVKHVTPHSFKHMHISYLQSNEMHIAVKDIMTRVGHANFETTMGYTHN
                     ISRSQENTVTALNQFVEKHNFHFKELKSYTCKYSRMIEQFIETCDDNNKVELSVDEFK
                     DLLHLGSRYSPKHIVSNLLLKIKKDIVKYHPQFDIKIVKLSGNQIKGFSISW"
     gene            complement(4943..5176)
                     /locus_tag="VXM85_001848"
     CDS             complement(4943..5176)
                     /locus_tag="VXM85_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1346374.1"
                     /translation="MEIKYVSVENSVAEWADKKSILKRWEGLNLYTLNRWIKEMRENK
                     RFSLYVINPTHKLVFINLEGFECFLRWKQKRTK"
     gene            complement(5478..5699)
                     /locus_tag="VXM85_001849"
     CDS             complement(5478..5699)
                     /locus_tag="VXM85_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1346375.1"
                     /translation="MYRRLRDLREDHDLTQKQIATILSFTNSAYAKIERSEHALTADV
                     LVKLSDFYDVSTDYLLGLTDFPDKIRFRK"
     gene            complement(5956..6429)
                     /gene="tnpA"
                     /locus_tag="VXM85_001850"
     CDS             complement(5956..6429)
                     /gene="tnpA"
                     /locus_tag="VXM85_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEV1346376.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            complement(6587..>6907)
                     /locus_tag="VXM85_001851"
     CDS             complement(6587..>6907)
                     /locus_tag="VXM85_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1346377.1"
                     /translation="LLFWDSLAILLTMFINDYRRRDIVIRFVLTPLGFTAPVFYLIDS
                     APSIVRWIGQLNPLTYQLTILRNFYFKNSTTLELVFLLLTSLLVLISVSFIIPKIKLI
                     LIER"
BASE COUNT         2074 a         1411 c         1222 g         2200 t
ORIGIN      
        1 tcagtaaaaa ataaccgatc tcatttctga gaatcggttt tctaaataaa tcaagctgag
       61 catttacata acataaatta tgtaaatgct catactacaa caacaaatct ttgacttttc
      121 caatttcact ttttggaata accaggtgaa tcatatcacc cagatacatt ctggttgagc
      181 cgttaactgt ttggctcttg ccattatgga cttgagttgt gatgaggacg ttgtgtggta
      241 agttgagttc atgaacttgt ttcccagcaa ttttatcaga aactggtatt tcgataagtg
      301 taacttctcc ttcgctagat acttcttctg gaagcatttt ttccagcatg gcttcataga
      361 ctggcgtacc tttgagcaaa tccatgataa tataagaaac aagagtgaca agaccaagtg
      421 gcatgaggtt gcgaatatct cctaccatct cagttacgag gatcatagcg gttaagggag
      481 cttttgatat ggctccaaaa tagccactca ttcctagaat gacaaatata gggaattgct
      541 cttgactgac aagtccaaga ttgacacaga taacaccaac taaggcacca agcaaagaac
      601 caagagctaa aatggggagg aaaattcctc ctggcagtcc acttccatag ctaatcatac
      661 tccaaataaa gcggatcaaa aagtaagcta ataaaacttg gaaactaaaa ttttgttcag
      721 ttaaagaaag gacaagctga tttccgccac caatgatttg aggtaagaag attccgactg
      781 gtatgataag gataaaagcc aagatgggat aataagccct atccaaatgg attttttgac
      841 caatcaagtc ataaactctt ccaacgttta atacagcttt ctcatagaga aaacctgaaa
      901 atccaaggaa aattcccatg acgagatata tccaatactg gtctagggtc atgggaggaa
      961 tgttatctgg catatccaat actggtgtca aaccgaacat gagtagagac acaaagtttg
     1021 ctacgatgct ggctgctaga gttgagaccc agaaaaagcg cgaaaaatgg tgatagactt
     1081 cttctacaac aaagagaagt gctgcaatag gagcattaaa ggctgcggct aaacctgctg
     1141 cagctccact agcaatcaag gaacgttcct ctactggact ggatttgagc cacttggcaa
     1201 ttcctttacc accaactgct ccaagttgaa tgctgggtcc ctctcgaccc agcatgagtc
     1261 cactggcaat agcaagaata cctagcacat attttttcca aagaatgccc caccagttga
     1321 gggacatgag gcctttcagt tcggcttcga cttgaggaat tcctgagcct ttaatatctt
     1381 tttctgaccg tgttagtttg gcactgagcc aacagatgag tatataaaac aaaaccagta
     1441 caaaaagatt gcgcactaag tacccttgat cttgataaac tccttgtatc aggtggaagc
     1501 ccttttcaat taagaaacga aaggatccga cgataattcc aacgatgagg ccgacaatga
     1561 ttcctcgacc aacttgggat agtatagtgc tggatgcaaa ggcgaattct ttcttagaac
     1621 ggactatttc cgattgttcc tccatggtat ttcctttcaa ttaactttct tttttaccta
     1681 aaaccagtga tttaacgggt tcaaagctgg ttcggtgaat tggggtaact cctagttttg
     1741 tgaggccttc cagatgttta gctgtgccat atcctgcatt agtagcgaaa tcatagccag
     1801 ggaactgctg atcgtattcc ttcatcaatt catcacgtgt taccttggct actatagatg
     1861 ctgctgcgat agagagggag ttggcatctc ctttgataat ggaggtttgt gaaatgggca
     1921 agtccagttt catggcatca atcaaaaggt gctctggttg agggctgagc tgggagattg
     1981 cttcttgcat ggctagtttg gttgcttcat agatattgac ttggtcgatg acctgattat
     2041 ctatgatacc aattccaatc gacaaggctt ggtcttgaac gacttggaaa atctccagat
     2101 gtttcttttt aggaattttc ttgctgtcgt tgagaccttt aatcttacaa tttttaggta
     2161 aaataacggc cgcagcgact acaggaccag caagaggacc acgaccaacc tcatcaatac
     2221 ctgcaattaa ggtcaatcct tgcttataaa gttctttttc ataagaaagc atggattcca
     2281 agcgcaaatt ttcatctaat tcagcttgga tggctctttt acgcttgctg atttcctttt
     2341 gaactcctga gcgattatcc ttttcaagct ctaaaaaaat agggctttct aactccttga
     2401 ctgtcacaag gaattctttg atttctttaa tcgttgccat cgaggtcttc caatgtatct
     2461 aaggtatagt taccgagttt gccatcacgg acttccttca cgaagagact gtaaaaacgg
     2521 tcatagtcat cacggaaacc gagggcgcgg gtcatatcca taataatcac aggcgctttt
     2581 tcttcaattt tcatttgttt gaagcgttca gccagctttt ctggataatg ttctttgaaa
     2641 taattgatac caaaaatggt aacctcatcc ataggaagca actggtcttt gatagctcca
     2701 gtcaatgcca acttaagtgc aacagtttca tcctcaaact taggccagag aatccccggt
     2761 gtatccaaga tttccaggtc tttattggtt ttaagccatt gttgaccttt tgtgacccct
     2821 ggcttgtttc caacaacagc aatcttttta ccagccaaac gattcatcag agttgattta
     2881 ccagcgtttg gaatcccgat aatcatagta cgcaaggttt caatctgaat cccacgttct
     2941 ttctggcgag caatcttatc agccatgagc ttcttggccg catctgttac aacttttaca
     3001 gtcacttgct ctttggagtt gatagctagc gtctggattc cttgtgattc aaaatactga
     3061 cgccattcct tggtcattgc tggatcagcc aagtcggcct tgtttaaaat caagagtttt
     3121 ggtttatcac caacaatctt ggtcaacata ggattttgac tagatagagg caagcgtgca
     3181 tctactaaaa tcgtcacaaa atcaacaaat tttaaattct cctgcacctg tcgacgagct
     3241 ttagacatgt gaccaggaaa ccattgaata gtagccattg agttttttcc tttcgtagca
     3301 agggtttagc acccctcatt tattttacta tcgtctaaac accaagcgaa caccaaaact
     3361 accacgaaat ggaaaaacct ttgatttgat tcccgcttaa tttcacaatc tttatatcaa
     3421 actgtgggtg atatttgaca atatcttttt tgatttttaa cagtaaattt gaaacaatat
     3481 gtttgggtga gtaacgtgaa ccaaggtgta ataagtcttt aaattcatca acacttaact
     3541 ctactttatt gttatcatca caagtttcaa tgaattgttc aatcattctc gaatatttac
     3601 aggtgtaact cttcaattct ttaaagtgaa aattgtgctt ttctacaaat tgatttaaag
     3661 cagttacagt attttcttgt gaacgactga tgttatgggt gtagcccatc gtggtttcaa
     3721 aattcgcatg accaactcta gtcataatat cctttacagc tatgtgcatt tcattacttt
     3781 gaagataact aatgtgcata tgtttgaaag aatgaggggt cacatgcttg acccatttaa
     3841 aaccataatc cttctgacaa ttttttaaca acttctcctc aaccctcttc aaaacttgac
     3901 gaaatgagtg agaagttatc ggagaaccat actcagtttg aaatacacta tcacactttt
     3961 tgaaagcagg actaggattt tgttccatat aggcttcaaa ttcctgattt ctttttatag
     4021 ttctctggat tgcttcactc gctgctattg ggagggctac ctctctatca gcattgacgg
     4081 tcttagtcgt atcaaaataa aattcatcaa cttttaaatc atgatattgg agagccttat
     4141 caatatgtaa aatcccctta tcaaaatcaa catctgatgg taaaaaagca gcctctccaa
     4201 tccgaatccc tgtcagtaat aatacaatag caaggtcata atagctagca tttctacact
     4261 gccctaaaac atcaaaaaag gcttgcaatt catgaattgc aagaaattta gcatcaagcc
     4321 tttttcttga ttctctcttt ttctccagag gcacatctat tttaacagcg gtcattggag
     4381 agtacttaat gacattgtat agcacaccgt gattaaatat cttgttacac gtacttttta
     4441 tatgtgtcat tgttgaaggt gaagcgtcgt atttatccag atatttatta agacaatttt
     4501 tcatcagtag tggtgtaatt cgatcgagta aaaattcatc accgataatt ttaccaagcc
     4561 gttctatcac caactcttcc ctttttattg tctggggctt gacagatacg caccaagttt
     4621 gaaaccaact ctcttttaac tctccaaaag ttgtaatcat ttcaggattg tactgacttt
     4681 caagggaaga ggtcagtttt tctattttat ccattacctc tctttcagct tgctttcgtg
     4741 ctctactagt atttctagta tagctgacag ttactgttct ccacttatca gtaagtggat
     4801 ctttatattt ttcaaccact tgatacaagg gttgtccttt tgaatttgtt ttagttacat
     4861 aatacataat ttaattcctc gaaaagaaat aaatcattgt tgtatagaat aaataactac
     4921 tctatattat accatgactt ccttattttg tccgcttttg tttccatctc aaaaagcatt
     4981 cgaatccctc aagatttata aacaccagct tatgtgtagg attgatgacg tataaagaaa
     5041 atctcttatt ttctctcatt tctttgatcc atctgttaag agtgtagaga ttcaaacctt
     5101 cccatcgttt cagtatgctt ttcttatccg cccactcagc taccgaattt tcaacagata
     5161 cgtattttat ctccatatta tcatcctttc gaataatttc ttctccctcc atggcatgac
     5221 tgatggggtc atcataagaa tctcacttac tgctctttta cgacggcagc ctgaacgttc
     5281 agaagtatca ttgtatatca tttgcatcat ggcagataga gtatcgctct attttattgg
     5341 tgattgtccc cgctcctatc ttggcgaatc aagcaaaact gctccacaaa tgaggaaagt
     5401 accattttag gctattcagt tgtcaaaggg caaataaaaa atctcactca ttttcaaact
     5461 ctatgaaatt gaggagatta ttttctaaag cgaattttat caggaaaatc agttaatccc
     5521 aataaatagt ctgtactgac gtcatagaaa tctgagagtt taaccaatac atcagccgtc
     5581 aacgcatgct cactccgttc aattttggca taagctgaat ttgtaaacga aagtattgta
     5641 gctatttgct tttgggtcag atcatgatcc tccctcaaat ctctcaaacg tctgtacatc
     5701 tagctttctc ctaagaaaaa gtatagacca tatattgtaa ctatttagga tattgtccaa
     5761 atcggacaac aggaatttta ccatccttta tatagacttt tatttttatg tatcaagttt
     5821 tttaaatcaa aaccaccagt gtgaactggt ggtttgttct gcggctataa gccgcttcta
     5881 ccggccaggg ccaaaggccc accgaaatag cttcctcgcg caccactttc ccgagcaggt
     5941 gctaaagcac cttagttact tcctcttatt tatttcgcca gtaaacggat ctactgactc
     6001 gaataacgtg agctggtctg ctactctgtc ttcttgtaat tgattctgaa tatattcagc
     6061 tatcactttc tgattacggc ctaccgtatc tacataatag cctctacacc aaaacttgcg
     6121 attgccatat ttgtatttta aattcgcatg cttatcaaaa atcatcaaac tgctcttgcc
     6181 ctttaaatag cccataaagg acgaaacact aagtttcgga ggaatactga taagcatgtg
     6241 aatatggtct gaacaagcat tcgcttcatg gattattaca cccttacgct cacataagtc
     6301 acgtatgatt cttccgatac tagctttgta tctgccataa atgatttgac gacgatattt
     6361 gggtgcaaaa acaatatgat atttacaatt ccatgtggta tgtgataaac tttgattatc
     6421 ctctctcatg aggtacctcc tgtatgatat gttgtagtgg cggagaaacc acttctatct
     6481 tatcatttta ggaggttctt tttgttacca cgctaaaagc tctatggaac cactagcata
     6541 gctagtggtt ttcgggagac aacaaaaaag ttggatgacc caacttttat ctttctatca
     6601 gtatcaattt tatctttggt ataataaaag atacagatat aaggacaagt aatgatgtta
     6661 acaataagaa aactaattcc aaagttgttg aatttttaaa ataaaagttt ctcaaaatag
     6721 ttaattgata agttaaggga tttaactgac caatccatct cacaatacta ggagcagaat
     6781 ctattaagta gaaaacagga gctgtaaaac caagcggtgt tagtacaaaa cgtattacaa
     6841 tatcacgtct tctgtaatca ttgataaaca ttgtaagtaa aattgcaagg gagtcccaaa
     6901 ataataa
//
LOCUS       DAVLRT010000092         6712 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLRT010000092 DAVLRT010000000
VERSION     DAVLRT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.092"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(94..679)
                     /locus_tag="VXM85_001852"
                     /pseudo
     CDS             complement(94..679)
                     /locus_tag="VXM85_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(655..831)
                     /locus_tag="VXM85_001853"
     CDS             complement(655..831)
                     /locus_tag="VXM85_001853"
                     /inference="COORDINATES: protein motif:HMM:NF015247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346378.1"
                     /translation="MQRNSGGKPTTELPSFIIRCLPVHLTYDNNYFNDTYQGIPIGGY
                     TQIVEKCWIMKILM"
     gene            complement(797..1267)
                     /locus_tag="VXM85_001854"
     CDS             complement(797..1267)
                     /locus_tag="VXM85_001854"
                     /inference="COORDINATES: protein motif:HMM:NF013739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEV1346379.1"
                     /translation="MYDYLVVGAVFAHEAALEGKKVKVIEKRNHIAGNIYTREEEGIQ
                     VHQYGAHIFHTSDKEIWDYVNQFAEFNRYTNSPVANYKGEIYNLPFNMNTFNKLWGVV
                     TPAEVQAKIDEQRAILNGKTPENLEEQAISLVGTDIYEKLIKDYTEKQWGQTNY"
     gene            complement(1330..2181)
                     /gene="rfbD"
                     /locus_tag="VXM85_001855"
     CDS             complement(1330..2181)
                     /gene="rfbD"
                     /locus_tag="VXM85_001855"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1346380.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            complement(2247..3296)
                     /gene="rfbB"
                     /locus_tag="VXM85_001856"
     CDS             complement(2247..3296)
                     /gene="rfbB"
                     /locus_tag="VXM85_001856"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1346381.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPGVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(3309..3902)
                     /locus_tag="VXM85_001857"
     CDS             complement(3309..3902)
                     /locus_tag="VXM85_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV1346382.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDL"
     gene            complement(3903..4772)
                     /gene="rfbA"
                     /locus_tag="VXM85_001858"
     CDS             complement(3903..4772)
                     /gene="rfbA"
                     /locus_tag="VXM85_001858"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV1346383.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLPRFKELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLTLAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(4799..5899)
                     /gene="glf"
                     /locus_tag="VXM85_001859"
     CDS             complement(4799..5899)
                     /gene="glf"
                     /locus_tag="VXM85_001859"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEV1346384.1"
                     /translation="MYDYLIVGAGLSGAIFAHEATKRGKKVKVIDKRDHIGGNIYCEN
                     VEGINVHKYGAHIFHTSNKKVWDYVNQFAEFNNYINSPVANYKGSLYNLPFNMNTFYA
                     MWGTKTPQEVKDKIAEQTAGMKDVEPKNLEEQAIKLIGPDIYEKLIKGYTEKQWGRSA
                     IELPPFIIKRLPVRLTFDNNYFNDRYQGIPIGGYNVIIENMLKDVEVELGVDFFAHRE
                     ELEASANKVVFTGMIDQYFDYKHGELEYRSLRFEHEILDEENYQGNAVVNYTEREIPY
                     TRIIEHKHFEYGMQPKTVITHEYPADWKRGDEPYYPINDEKNNAMFAKYQEEAAQNDK
                     VIFCGRLADYKYYDMHVVIERALEVVEKEFSN"
     gene            complement(5902..>6712)
                     /locus_tag="VXM85_001860"
     CDS             complement(5902..>6712)
                     /locus_tag="VXM85_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEV1346385.1"
                     /translation="KPIFVLLGSVIAIEIYTMLDTTMIGAMVGTTEVGYYTNSMKLVK
                     LLITVITAIGGVLLPRLSDYYSKGEFEKLNHIVDKVFRIMLFLFLPAQVALILVAPVI
                     MPILFGDSFIPAVLTLQLTSLLICTLGFSNLFGTQILLTFGDERKLLISTILGALSNI
                     ALNLILIPVMAQNGAALASVISETIVTLTAYYYAKKRVTIRFDLGFYLPTVVSTIIMG
                     GAIYVVMQLYIGDIEKMLFSTLLGGVVFLLINVFMKNPIIYEFTGIIKRKR"
BASE COUNT         1965 a         1479 c         1117 g         2151 t
ORIGIN      
        1 catatagtca aggaattttt aatactattt ctaaataata actggtagtc agttttactt
       61 gtttatactc ttcgaaaatc tcttcaaacc gcgtcagctt ccatctgcaa cctcaaaaca
      121 gtgttttgag caacctgcgg ctagcttcct agtttgctct ttaattttca ttgagtatga
      181 atctaactca tttctcacgc actgcaaagc tgctccaatt acttggtgca tatcgtaata
      241 acgatagtgt cctaagcggc caccaaagat aacattccct tgctcatcag caagtttttt
      301 atatgattta tacaaatgat tattacgatc attattaact ggataataag gctcatcacc
      361 tttttcccat gttttagaat gttctattag taatgatagt ctttgcttga ctcccaaact
      421 caaaatgttt gtgttcaata atgcgagtat atggggtttc tgcatccgta tagttcacaa
      481 ctgcatttcc ttggtaattt tccatatcca aggtctcatt ttcaaaacga agactacggt
      541 actctagttc gcccaactta tagtcaaaga attcatcaat cataccagta aagacaatct
      601 taggaaaatc tttcagatat tgctctttgt tcacaaagaa atcaacattt gtttctacat
      661 caatattttc atgatccaac atttttcaac tatttgagtg tatccaccaa ttggaatccc
      721 ttgataggta tcgttaaaat agttgttatc ataggtcagg tgtactggta aacagcgaat
      781 aataaaggat ggaagttcag tagttggttt gcccccactg tttctctgta tagtctttga
      841 ttaatttttc gtagatgtct gtacctacaa gagaaatcgc ctgttcttcc aaattttcag
      901 gagttttacc atttaaaata gcacgttgtt catcaatctt agcctgtact tctgctggcg
      961 ttacaactcc ccagagttta ttgaaggtat tcatattaaa aggaaggtta taaatctcac
     1021 ccttatagtt tgcaacagga gaattggtat aacggttaaa ctctgcaaac tgatttacat
     1081 aatcccagat ctccttatca gaagtatgga agatatgagc accatattga tgaacttgaa
     1141 ttccttcctc ttcacgagta tagatattac ccgcgatatg atttcgtttt tcaataactt
     1201 ttactttttt tccttctaag gctgcttcat gggcaaagac tgcaccaaca acaagataat
     1261 cgtacatctt aagctccttt ggagttcatt atctgcctct tttattagac tagaaaatca
     1321 ttctactact tatctcactt cttgtttgta aaattcttgc aatgcatctt gccaagttgg
     1381 aataacaaat ccagtagctt tggctttggc caggctcatc gttgagttta gcggacgttt
     1441 agctttggct ggaaattgac tggaatctac tggcttgact tcgacatctg tatctttcaa
     1501 aatttcaact gcaaaatcat accatgttgt gtcttctgtc gcatcatttg acaaatgata
     1561 ataaccaaat tccttacgat tttcagctag gtaggtcatg aactcagcca aggtacgagt
     1621 ccaagtcgga cgaccgtact ggtcatttac aactgttaaa gtcttatgag ttttcgcaag
     1681 attttgcatg gtaaaaacga agtttttgcc ataatttcca aatacccagg cagtacggat
     1741 aatatagaaa ttagacacat gcttctcaac taactcttcc cccatacgct tagtgcgtcc
     1801 atattctgtc tgtggatctg gtcggtcatc aacttcccac tcttgtccaa ctggtttctt
     1861 accgtcaaag acatagtccg tagaaatata aactagagtt gcaccatgct tttcagatgc
     1921 ttttgcgaca ttttttgtcc ccgtcacatt gatggcgaag tccaactctt ttccttcatc
     1981 ctctgctgca tcaacagcgg tgtaggctgc acagtggtag actaaagtcg gtttcacctc
     2041 ttcaaaaact ttctcaacca tttcttcatt ggtaatgtcc atctcagcca catctactgc
     2101 cacgtattct tcattacgtt catccaataa atagcgaagt tccgttccta attggccatt
     2161 tgcccctgta attaaaatca tctctagacc tttctaatat gtaactcttc tcaatatagc
     2221 aataaaaagc agctatttcc tgctttttat actgtaataa tctcctgagt cttagcataa
     2281 ttggcttcaa cagcttcttt ttctgctttc caccattctt ggttatctgt ataccacttg
     2341 attgttgcct tgagcccagc ttcaaagttg gtaaattcag gtttccaccc caactcatca
     2401 cggagcttgc tggcatcaat cgcatagcga aggtcatgtc ctgcacggtc agtcacatga
     2461 tcataggcat ccgtagcttg tcccatttcc ttaaggataa gttccaaaac ttccttattg
     2521 ttcttctcac catcagcccc aatcaagtag gtttcaccga tttgcccttt tgtcaagatt
     2581 gtccaaactc ctgaagaatg gtcattggta tgaatccagt cacgaacatt cttaccttca
     2641 ccgtaaagtt ttggcttaat tccacttagg atgttagtaa tctgacgtgg gatgaatttt
     2701 tcgatatgtt gataaggacc gtagttattt gaacagttgg aaatcgttgc cttgactcca
     2761 aaagaacgca cccaggcttt gacaatcaaa tctgaggctg ccttggttga tgagtacgga
     2821 gagcttgggt tgtattttgt ttcagcagta aatttctcac ctggtccttc accatgacct
     2881 ggcaaatctt cgcgtaaagg gagatcccca taaacttcat ctgtcgatac atggtggaag
     2941 cgaatatcat acttacgagc agcttctaaa agagtatagg ttccgatgaa gttagtatga
     3001 ataaatggcg atggatcatt gagcgaatta tcattgtggc tttccgctgc ataatgaacg
     3061 atagcatctg cttgagcagc caacttgtct accaactccg catcagcaat gtcaccaaca
     3121 actaactcaa cacgattacc taaaatttcc tcaatattcg cgcgatttcc agcataagtc
     3181 aacttatcta ggactgtcac gtgaacacct ggaaagttct cgtaaacata atggacaaag
     3241 ttagaaccga taaagccagc tccacctgtc acgataatat ttttgtattc agtcatattc
     3301 tttccttttt acaaatcttc ttttttcaaa ggttttacat ccttaagtag gggatgatgt
     3361 ttatctgctt ctgaaacctc tgcttctgca atattttccc attcaatacc aaggcttgga
     3421 tcagcgtagt tcacaaaggc atacttgggt ttgagctcga gagcccagta atcattgacc
     3481 agatagctat atgacactgt atctgataga acttggaagc cattagctac gcctcgagga
     3541 acaaagattc ccttgcttgc atcaattact gtctgatagg tattcccaaa ggtttcaccc
     3601 tcgcgtagat caacccaaga acccagaact ttccctccat ctgctacaga gatgtactta
     3661 tcccaaggct ctgcgtggag tcctcgaagg acatttttac gtgagaagga tacattgttt
     3721 tgcaattttc cttctgcaaa gaaagactct ggaaatccaa gtggaagcat tttttccttt
     3781 tggaaatttt ctttaaacca gccacgatta tctccatgaa cggggatatc aaactccaac
     3841 atgcctggaa tagcttcaac cttgcgtgcc gcaagcgtct taccgaaaaa attatctgtc
     3901 atctatgctt ctccaatcaa acggagcaga tactgtccgt attcattttt cttaagtggt
     3961 tgggctaagg tcaatacatc ttcacgactg atataaccca tgcgataggc aatttcttct
     4021 aagtttgcta cctgaacatt ttgcatccgt tggactgttt cgatgtactg tgaagcctct
     4081 agtaaacttt catgagttcc agtatccagc caagcaaagc cacgtcccat aagctcaaca
     4141 gacaaatcac cacgatctag gtaagctttg tttacatctg taatttccag ttcaccacga
     4201 ggacttggtt taatactctt ggcaatctct actacatcat tatcatagaa atagagacct
     4261 gtaactgcat agtttgaacg aggttgctct ggcttttctt caatagaaat agccttcata
     4321 tcctgatcaa actcaaccac accaaagcgc tctggatcct tgacatggta gccaaaaaca
     4381 gttgctcccg actccttact cgctgccttt tgtagcatct tggaaagccc agaaccatgg
     4441 tagatattat cacctaagat tagcgcaacg ctatcatcag aaataaactc ttccccaatg
     4501 ataaaggctt gtgccaaacc atctggactt ggttgctcag cataagaaag tttaatacca
     4561 aactcggaac catcttgaag aagctcttta aaacgaggca aatcttgagg agttgagata
     4621 atcaaaatat ccctaatccc agccaacatt aatgttgaaa gtgggtagta aatcatcggt
     4681 ttatcataaa ccggcataag ttgttttgat gcggctcgag tcaaaggata caagcgagtt
     4741 cccgaaccac ctgctagaat aatacctttc ataataagca acctttcttc gttgtttttc
     4801 aattactaaa ttctttctcc acaacctcaa gcgcccgttc aatcaccaca tgcatatcgt
     4861 aatacttata atctgctaaa cgcccacaaa agataacttt atcattctgc gctgcttctt
     4921 cttggtattt agcaaacata gcattatttt tctcatcgtt aataggatag tagggctcgt
     4981 ccccacgctt ccagtcagct ggatattcat gcgtgataac tgtctttggc tgcattccat
     5041 attcaaaatg tttgtgttcg atgatacgag tataagggat ctcacgctct gtatagttca
     5101 ctacagcatt cccttgataa ttttcctcgt ctaaaatctc atgctcaaaa cgaaggctac
     5161 ggtattctaa ctctccgtgc ttgtagtcaa aatattggtc gatcattcct gtgaagacaa
     5221 ctttgttagc agatgcttcc aactcttcac gatgagcaaa aaagtctact ccaagttcaa
     5281 cttctacatc tttcagcata ttttcgatga tgacattgta accaccaata ggaatccctt
     5341 ggtaacggtc attaaaatag ttattatcaa aggttaaacg aactggaagg cgttttatga
     5401 taaatggagg aagttctatc gctgagcgtc cccattgctt ttctgtataa cccttgatca
     5461 acttttcata gatatccgga ccaatcaact tgatagcttg ttcttccaga tttttcggct
     5521 caacatcctt cataccagct gtctgctcag caatcttatc cttcacttct tgtggagttt
     5581 ttgtgcccca catagcatag aaggtattca tattgaaagg aaggttataa agacttccct
     5641 tatagtttgc gacaggtgag ttgatatagt tgttaaactc agcaaattga ttgacgtagt
     5701 cccagacttt tttattagaa gtatggaaga tatgggcacc atatttatga acattgatac
     5761 cttctacatt ctcacagtat atgttccctc caatgtgatc gcgtttatca atcactttta
     5821 ctttttttcc acgctttgta gcttcgtgtg cgaagattgc tccagacaaa cccgctccaa
     5881 cgattaaata gtcgtacata attatctctt tctctttata atacctgtaa attcatatat
     5941 aataggattc ttcataaaaa cgttaattaa aaggaataca acacctccta acaaagttga
     6001 aaataacatt ttttctatat ccccaatata caattgcatt actacatata tagctccacc
     6061 catgattatt gttgaaacaa cagttggcaa ataaaatcct aagtcaaacc taattgtcac
     6121 acgttttttc gcatagtagt atgctgttaa agtaacaatt gtttcactaa tcacagatgc
     6181 caacgctgct ccattttgag ccattactgg aatcagaatt aggttcaaag cgatattact
     6241 aagcgctcct aaaattgtgc taatcaaaag ctttcgctca tctccaaatg ttagtaatat
     6301 ttgagtccca aataaattac taaaccctaa tgtacatata agcaaagaag taagttgaag
     6361 tgttaacact gctggtataa aactatcacc aaataaaatc ggcataatta ctggtgcaac
     6421 tagaattaat gcaacttggg ctggtaaaaa aagaaacaac atgattctaa aaactttatc
     6481 aacgatgtga tttaattttt caaattctcc cttagaataa taatcactta gtcgtggtaa
     6541 aagcactcct ccaatagcgg taatcacagt tatcagcaat ttaactagct tcatagaatt
     6601 tgtataataa cctacctcag tcgttcctac catagcacca atcatagtgg tatccagcat
     6661 agtatagatt tcaatagcaa tcactgaacc caacaaaaca aaaataggtt tt
//
LOCUS       DAVLRT010000093         6020 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLRT010000093 DAVLRT010000000
VERSION     DAVLRT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.093"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            152..1921
                     /locus_tag="VXM85_001861"
     CDS             152..1921
                     /locus_tag="VXM85_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV1346386.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     VAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            1945..3900
                     /locus_tag="VXM85_001862"
     CDS             1945..3900
                     /locus_tag="VXM85_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1346387.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESI
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3902..4339
                     /locus_tag="VXM85_001863"
     CDS             3902..4339
                     /locus_tag="VXM85_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1346388.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4401..5537
                     /locus_tag="VXM85_001864"
     CDS             4401..5537
                     /locus_tag="VXM85_001864"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV1346389.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1835 a         1007 c         1236 g         1942 t
ORIGIN      
        1 gtacacctga tatgatgcgt tttataattt ttaagccttt ttgcccagcc tcgtcaaaag
       61 taatgttttg acacaaaatc tgtgacaaaa ctttagtttt aaaggttttt aactttgtat
      121 atactagttt taagaaaagg aggatgatct aatggaagaa aaagtatcat tgaaggtcag
      181 ggttcaaaaa ctagggacat cgctttcaaa tatggttatg cccaatattg gagcatttat
      241 tgcttgggga gtattgactg ccctctttat cgctgatggc tatctgccaa atgaacagtt
      301 agctactgtt gttggtccta tgttaacgta tttattgcca atcctgattg gttacacagg
      361 tggatatatg atccatggcc aacgtggtgc cgttgtagga gctattgcta ctgttggtgc
      421 aatcacaggt tctagtgttc ctatgtttat cggagctatg gtaatgggcc cactgggagg
      481 atggactatc aagaaatttg atgagaagtt ccaggaaaaa attcgtcccg gatttgaaat
      541 gttagttaat aacttctcag ctggtctcgt tggttttgca ttattgcttt tggctttcta
      601 cgcaatcggt ccagtcgtat cgactcttac tgtagctgtt gggaatggtg ttgaggctat
      661 tgtcaatgct cgcctccttc ctatggctaa tattatcatc gaaccggcta aagtcctttt
      721 tctcaataat gccctcaatc atggcatttt tactcctctg ggagtagaac aggtagctca
      781 agctggtaag tcaattctct tcctattgga agctaatcct ggaccaggcc tgggaattct
      841 attagcttat gctgtattcg gtaaaggttc tgctaaatct tcttcttggg gggcaatggt
      901 tattcatttc ttcggaggga ttcatgaaat ttactttcct tatgttatga tgaagcctac
      961 tctattttta gctgctatgg caggaggtat ctctggaact tttacttttc aactcttaga
     1021 cgctggtctt aaatctccag cttcaccagg ttctattatt gcgattatgg ctacggcgcc
     1081 aaaaggtgtt tggcctcatc taaatgttct tttaggtgtt ttagtggcag cagttgtttc
     1141 tttccttgta gcagccctta ttcttcatgc agacaagtca actgaggatt cgctcgaagc
     1201 tgctcaggcg gctacccaag cagctaaggc tcagtctaaa ggtcagttag tatcaacttc
     1261 tgttgatgca gttgtttcga cagactcagt ggaaaaaatc attttcgcct gcgatgctgg
     1321 tatgggaagc tctgctatgg gagctagtat tcttcgagat aaggttaaaa aagcaggtct
     1381 agagattcca gtatctaatc aggcaatctc aaatttgctt gatacaccaa aaacattaat
     1441 tgttactcag gaagaactga caccaagagc taaagacaag agtccaagtg ctattcatgt
     1501 ttctgttgat aatttcttag cgtcccctcg ttatgatgaa attgtagctt cattaacagg
     1561 agcttttcca atagcagaaa ttgaaggaga tataccaact tcagcaccag tagatagtca
     1621 ggaaattgac cttaaccata ttgatgctgt agtagttgct tatggtaaag cacagggaac
     1681 tgcaactatg ggctgtgaaa cgattcgggc tatctttaga aacaagaata ttcgtattcc
     1741 agtttctact gccaaaattt cagaattagg tgaatttaat tctaaaaaca taatgattgt
     1801 aacaactatt tctttacagg cagaagtgca gcaagcagca ccgaattctc aatttcttat
     1861 tgtggatagt ttagtaacaa caccagaata tgacaaaatg gctgctagaa tgtacaaata
     1921 gaactagagg tttctaaatt acgaatgcta ttaaccaaac gagaagaaca attattgaag
     1981 gctttcctac atgtagggaa gctttcaatg caagatatga ctgaaatctt acaggtttca
     2041 tctagaacaa tttatcgaac tttatcagat ttgacagata gcatggagca atatggaatc
     2101 gaaataacga agcatgggaa atactatatt ttgactggag agttggatga tttgccgaca
     2161 gaacttgaag tgttagttga gtatagtccc caagaaagac aagagttgat tacctatcgc
     2221 cttctgactg agagcggttt tgtcaccaat gaagcattgc aagagtgcac gaaagtcagt
     2281 aatgtaacta ttattcagga tatttcagat attgataagc gtcttttaga ctttgatctg
     2341 aaaattgaac gacaaaaagg ttatcggatt tctggtgatt cagttggtaa gagaagattt
     2401 ttggctattt tactgacaaa ctgtatctca gtagcagatt tttcaaccgg taattttggg
     2461 agctttgata ttttagaagc agatagaact aggctggcca gtcagattgt taataagcaa
     2521 ctgtcaggtt ttccagatat ggatgctagg atgaagatgt tttttgcgat cttgttatct
     2581 cttataggtc aggagcaaaa cattgaaaat tcacctaata ctagtaagca ggctttggaa
     2641 atttctcaaa aaatttttca agcttactct aagcagactg cacaatttta tagtattcag
     2701 gaaattatct attttgcgag catcttggat gaattaatca ttaaacgtca ggacaatccg
     2761 ctctttacgg agaaatttga tggtgaattt ttctacaata tttcaaatct gattgatacg
     2821 gtttccatgt ataccaagat tgactttttt aaggacaagg ttttattcaa ttttcttttc
     2881 catcatattc ggctcagttt aggcgtccct atcctttttc agggtgaaaa tttgccagaa
     2941 tctatccaga ttttagttga aaggaataaa tttctttata cagtcatcag tcttttagtg
     3001 aatgatattt ttccgaaata tcttcataca gagtatgagt atggcatgat tgccctacat
     3061 tttatctcta gcttaggccg tagtccagag atttatccag tccgtgtttt gcttttaacg
     3121 gatgaacgtc gggtcactag agatttatta gtcagtaaaa ttaagagtgt tgctcctttt
     3181 gtagagttga tagatattca gtctctagta gattaccaca gtattgatct cagtcagtat
     3241 gattatattt tatctaccaa gccgctgact aatcaggaaa tcgatgtaat ttctagtttt
     3301 ccaaccgtca aagaattgct tgaattacag gaacgacttc agtatgtaca ggcacatcgt
     3361 acaattgtcg cgcgtgatgc tatcgctcca gagaaaagtt atgacttgca agattattta
     3421 atatctagta gtcagctttt gagtcaattt gagttggttc aattggagaa taatcaatca
     3481 tttgagcaca cggtagaaca aatcatccaa tatcagaaga atgtgagtga cagagattac
     3541 ctaacaagaa aattgttatc tcacttccag aatagtccta tggctattcc taatactggt
     3601 ctggtgcttt tacatagtca gtctagcaaa gtaacaacaa atagttttac tatgtttgaa
     3661 ctcaaactac ctatctccgc attgtcaatg aaacgagagg aagaagaggt caaaaggtgt
     3721 ctgctaatgc taatgtctaa agaagctagc gaggaagcga gagatttaat gacagctatt
     3781 agtcagtcga ttattgaaaa tcatctttat acagagattt acaagacggg aaatcaatcc
     3841 attatttatc agatgctaaa tactattttt aacgaaaaaa ttaagaaatt ggagaactaa
     3901 tatgaaactt gaaaaacatt tgattaagct taataaacaa ttttctaaca aggaggaagc
     3961 tatttgttat tgtgggcaag ttctttatga gggtggatat gttaatgaag actatattga
     4021 agccatgatt gagcgagata aagagctatc tgtttacatg ggtaacttta tcgccatacc
     4081 gcatggaaca gatgcagcaa aaaaggatgt actcaagtct ggtattacag tcgttcaagt
     4141 ccctagaggg gttgattttg ggaatgtatc taaccctcaa gtggcaacgg ttctttttgg
     4201 tattgctggt attggtaatg aacacttgga aattattcag aaaatttcta tcttctgtgc
     4261 agatgtagat aatgttctta aactagcaga tgctcagtca aaagaggaag tattgcgctt
     4321 atttgatgct gttgaataat tgaatttcgt catttgtcat ctagtatata tgtccctcaa
     4381 ataggaaaag gagaaattga atgaaacatt ctgttcattt tggtgccggt aatatcggtc
     4441 gtggttttat aggtgaaatt ctatttaaaa atggtttcca tattgatttt gtggatgtca
     4501 ataatcagat aattcatgct ctaaatgaaa agggcaagta tgaaattgaa attgcacaga
     4561 aaggacagtc tcgtatagaa gtaactaatg tggctggcat taatagcaaa gaacatcctg
     4621 agcaagtcat tgaagcgatt caaaagacgg atattattac tactgcaatc ggacctaata
     4681 tactcccttt tatcgccgaa cttctagcca aaggaatcga agctcgccga gttgcaggaa
     4741 atacacaggc attggatgtt atggcctgtg aaaatatgat tggcgggtct caatttcttt
     4801 atcaagaagt caagaaatat ttaagtccgg aaggtttgac atttgctgat aactacatag
     4861 gttttccaaa tgctgcagta gacaggattg ttccagcaca aagtcacgaa gattcccttt
     4921 ttgttgtggt cgagcccttt aatgaatggg tcgtggaaac caagcgtctt aaaaatccag
     4981 atttacgtct aaaagatgtg cattatgaag aagatttaga accctttatt gagcgaaaac
     5041 ttttttcagt caattctgga catgcaactt cagcttacat tggtgcgcat tatggtgcca
     5101 agacaatttt ggaagctctt caaaatccta atattaaatc tcggattgaa tctgtattag
     5161 ctgaaattcg gagtctcttg attgccaaat ggaactttga taaaaaagaa ttggagaatt
     5221 atcacaaagt cattatagaa cggtttgaaa accctttcat agtggacgag gttagtcgcg
     5281 tagctcgtac tccaatccga aaattaggct ataatgaacg attcatccgg ccgatacgtg
     5341 aattgaaaga actcagtttg tcatataaaa acctacttaa aacagttggc tatgcctttg
     5401 actatcgcga tgtaaatgat gaagaaagta ttcgattagg tgaattgttg gctaaacaat
     5461 cagtcaaaga tgttgttata caagttacag gtttagacga ccaagaattg attgagcaaa
     5521 ttgtagagta tatttaatct ttttcgaaaa tctcttcaaa tcaggttagc atcgctttgt
     5581 cttaggcata tgttgttcta tctacaacct caaagcagtg ctttgagctg actccgtcag
     5641 tcttgtctgc aacctcaaaa cactgtttga gttatctgcg gtaatctttc tagcttgtct
     5701 ttgatttttg ttgttattta taaggtaaaa gaagctggac aaaaagtctt caaaatcgga
     5761 aaaaggcagc ctatcgggtg ttcaaaaatc ttgataggat gtcctttatt atggaaagcc
     5821 ttattggatt ttctcctcag attgagtttt tgatcagctt tatgagatag gttttgctag
     5881 agatgtagcc catcatgtta tttttatgga cggtgggaaa attgttgaaa aaaataatgc
     5941 ccatcaattc tttagtcgtc caagagaaga acgaaccaag caattttgga acgaattctt
     6001 tcgaatgcga tctatatagt
//
LOCUS       DAVLRT010000094         5387 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLRT010000094 DAVLRT010000000
VERSION     DAVLRT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.094"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..692
                     /locus_tag="VXM85_001865"
     CDS             <1..692
                     /locus_tag="VXM85_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV1346390.1"
                     /translation="KKWLFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSIIDL
                     VIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLTKL
                     LKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFSDS
                     LWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDEQI
                     KLDKLKDELSS"
     gene            complement(1041..2027)
                     /locus_tag="VXM85_001866"
     CDS             complement(1041..2027)
                     /locus_tag="VXM85_001866"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1346391.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            2221..4689
                     /gene="gyrA"
                     /locus_tag="VXM85_001867"
     CDS             2221..4689
                     /gene="gyrA"
                     /locus_tag="VXM85_001867"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1346392.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            4689..>5387
                     /gene="srtA"
                     /locus_tag="VXM85_001868"
     CDS             4689..>5387
                     /gene="srtA"
                     /locus_tag="VXM85_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1346393.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSD"
BASE COUNT         1673 a          905 c         1178 g         1631 t
ORIGIN      
        1 agaaaaaatg gttatttgca gattattatg atacaacaat tattttactt gctttaatct
       61 ctgttattct ggtattactt ggatttgctg agatgactga tttggacaat ccgccttata
      121 gtattattga tttagtaatc tggggtgttt ttgtaattga ttatagttgg cgctttttta
      181 ttactaaaag aaaatggcgc tttattcttg aaaatatttt tgatttattg accattctcc
      241 ctttaaatgc tatttttaca gtgtttcgat taggacgtat cttccgctta gcaaggctga
      301 caaaattact gaaactgact cgtttactta gaataattgg acttacaggt aaactagaaa
      361 gaaaaattag tagattctta cgaacaaatg gattgattta tatcttgtat gttaatatct
      421 ttattgttct agtagggagt tcgattttat ccgttgttga agaaaaatcc ttctcagata
      481 gtctttggtg ggctcttgtg acagtaacta ctgttggtta tggtgatatt gttccagttt
      541 ccctttttgg gaaatggcta gcagttttac tgatgcttgt cggtattgga acaataggga
      601 tgctaacaag tgctttgacg aacttttttg taaaggataa tccagatgaa cagataaaac
      661 ttgataaact caaagacgaa ttatcgagct aaagaatatt aatagagaaa caatctgaaa
      721 aaatcgaaga actacattga atgataaaag acttagttga aaaaatatca taaaggtgag
      781 acaaaataaa acttgattcg aggagaaatc aagtaagttt tgacacaata gaacgcatat
      841 tatcaggtat aaaaacttga tactatgcgt tttatagaga aataaaataa aatctgaaca
      901 aatcaattag gaatttaaag cattttataa caacgtttta gagtaatggg gtgctatttc
      961 aacttcaacc tactataata cagaaaaaaa tacagaaaaa aacaactccc tgatgattca
     1021 aggagttgtc tatagttaaa ttagttttta gaagcttctt ggaattctgg atttttccat
     1081 gcttcgtcaa taatagcttg caattcttta gcagatgctt gcattttttg agtttctgca
     1141 tcgttcaatg gaatgtttac tggacgaacg ataccatgtg caccaacaac agctggttga
     1201 ccgataaaga cattctcaac tccgtattga ccttcttgga atactgaaag tggaagtact
     1261 gcgttttcat cgtcaaggat tgctttagtg atacgagcaa gggctactgc gataccgtag
     1321 tatgttgcac cttttttgtt gatgattgtg taggctgcat cacgaacacc ttcgaacaat
     1381 tcaatcaatt cagcttcttg aacattttga gtgtctttaa ggaattcttc aagatttaca
     1441 ccagcgatgt tagcgtgtga ccaaacagcg aactcagagt caccgtgttc acccatgatg
     1501 taggcgtgca ctgaacgagc atccacatcc aatttttcag caagtgcttg acggaaacga
     1561 gctgagtcaa gtgaagtacc tgaaccgata acgcgttctt tagggaaacc agagaatttc
     1621 caagttgagt aagtcaaaac gtcaactggg ttagcagcaa caaggaagat acctttgaaa
     1681 ccagattcaa caacttgagt tacgattgat ttgttgatag caaggttttt acctacaagg
     1741 tcaagacgag tttcacctgg tttttgaggt gcacctgcag tgatcacaac aaggtcagcg
     1801 tctgcacagt cagagtattg agctgcatag atttttttag gtgaagtgaa ggcaagggcg
     1861 tgactaaggt caagcgcatc accaacagct ttttcatgca attgtggaat ttcgataatt
     1921 ccaagctctt gtgcaattcc ttggttaaca agtgcaaaag cgtaagatga acctacagca
     1981 ccatcaccga caaggataac ttttttgtgt tgtttagttg aagtcattgt tttaaacatc
     2041 tccttaattt tattagggga ttttccctag acaacttcat tctatcactt ttaaaaaact
     2101 ttgtcacgaa tatgccttat agttctcgat gtaaacgttt tagtggttta gaggctgaaa
     2161 tagatgggaa tttatggtat aatgttgtta cttactaatt gtgaaatgag gcatttatta
     2221 atgcaggata aaaatttagt gaatgtcaat ctgacaaagg agatgaaggc aagttttatc
     2281 gactacgcca tgagtgttat cgtagcgcga gctcttcctg atgttcgaga tggcttaaaa
     2341 cctgttcacc gtcgcattct ctacggaatg aatgaattgg gtgtgacccc agacaaaccc
     2401 cataaaaaat ctgctcgtat tacaggggat gtcatgggta aatatcaccc acacggggat
     2461 tcctctattt atgaagccat ggtccgtatg gctcaatggt ggagctaccg ttacatgctt
     2521 gtagatggtc atgggaattt tggttccatg gatggagata gtgctgccgc tcaacgttat
     2581 accgaggcac gtatgagcaa gattgctctg gaaatgcttc gtgatatcaa caaaaataca
     2641 gttgatttcg ttgataacta tgatgccaat gaacgggaac ccttggtctt gccagcgcgt
     2701 tttccaaacc ttttggttaa tggagcaact ggtatcgcgg ttgggatggc aaccaatatt
     2761 ccacctcata atctgggtga aaccattgat gcagtgaagt tggtcatgga taatcctgaa
     2821 gtgactacca aggacttgat ggaagtcttg cctggaccag attttccaac tggtgctctt
     2881 gtcatgggga aatcaggtat ccataaggct tatgaaacag gtaaaggttc gattgtccta
     2941 cgttctcgta cagagattga aacgactaag actggtcgtg agcgtatcgt tgtaacagaa
     3001 tttccttaca tggtcaataa aaccaaggtg catgagcata ttgttcgctt ggttcaggaa
     3061 aaacgcattg agggtatcac agcagtacgt gatgagtcaa accgtgaagg tgttcgattt
     3121 gttattgaag tcaagcgcga cgcctcagcc aatgttattc tcaataacct cttcaaaatg
     3181 acccaaatgc aaaccaattt tggattcaat atgctcgcta tccaaaatgg tataccgaaa
     3241 attttgtctc ttcgtcagat tttggatgct tatattgagc accaaaaaga agtggttgtt
     3301 cgtcgtacac gttttgataa ggaaaaagcg gaagcgcgcg ctcatatctt agaaggtctc
     3361 ttgattgcgc tagaccatat cgacgaagtg attcgtatca tccgtgctag tgaaacggat
     3421 gcggaagctc aagctgagtt gatgagcaag tttaagcttt ctgaacgtca aagtcaagct
     3481 atccttgata tgcgtcttcg tcgtttgaca ggtttggaac gcgataagat tcaatctgag
     3541 tatgatgacc tcttggctct gattgcggat ttagcagata ttcttgctaa gcctgaacgt
     3601 gtttctcaaa ttatcaaaga cgaattggat gaagttaaac gtaaattttc tgataaacgc
     3661 cgtacagagt tgatggttgg acagatcttg agtctcgagg atgaggactt gattgaagaa
     3721 tcggatgtct tgattaccct ttctaacaga ggctacatta agcgtttgga tcaggacgag
     3781 ttcactgctc aaaaacgtgg gggtcgtggt gtccaaggaa cgggagtgaa agatgatgac
     3841 tttgttcgtg agttagtgtc aactagcacc catgatcatc tgctcttctt cacaaacaag
     3901 ggacgtgtct atcgtcttaa aggttatgaa attcctgagt atggtcggac tgccaaaggg
     3961 ctaccagtag tcaatctctt gaaattggat gaagatgaaa gtattcagac ggttatcaat
     4021 gttgagtctg atcgcagtga tgatgcttat ctcttcttta caacccgtca cggtattgtg
     4081 aagagaacta gtgttaagga gtttgccaat attcgtcaaa atggtctcaa agcgctgaat
     4141 ttaaaggatg aagatgagtt aatcaatgtc ttgttgacag aaggagatat ggatattatc
     4201 attggtacca agtttggtta tgcagttcgc tttaatcaat cagccgttcg tggtatgagc
     4261 cgtatcgcca ctggtgtgaa aggtgttaac cttcgtgaag gagacacagt tgttggtgcc
     4321 agcttgatta ctgatcaaga tgaggttctt attatcacag aaaaaggata tggtaagcgt
     4381 acagtcgcta ctgaataccc aacaaaaggt cgtggtggta agggaatgca gacagctaaa
     4441 attaccgaaa aaaatggctt gctggccggt cttatgactg ttcaagggga tgaggatttg
     4501 atgattatca ctgatacagg tgtcatgatt cgaaccaatc ttgccaatat ttcacaaaca
     4561 ggacgtgcaa ctatgggagt taaagtaatg cgcctggatc aagatgctca gatagtgact
     4621 ttcacaacgg ttgcggtggc agaaaaagaa gaagttggga cagaaaacga aacagaaggt
     4681 gaagcataat gtctcaaaaa aataataaaa agaaaaacaa gcgaaaaaat ctgctgacaa
     4741 atatcctagc aggatttctg atattactgt cactggcttt gatttttaat actcaaattc
     4801 gaaatatttt catagtctgg aataccaata agtatcaagt tagccaggta tcaaaagaaa
     4861 aattagaaga aaatcaggat acagaaggca attttgactt tgattctgtc aaagctatct
     4921 cttcggaagc tgttctaact tctcaatggg atgctcaaaa attaccagtt attgggggaa
     4981 ttgcaattcc tgaattggaa atgaatttgc cgatttttaa aggacttgat aatgttaatc
     5041 tcttctacgg agctggtaca atgaaacgcg agcaagtaat gggagaagga aattatagtc
     5101 tagctagtca ccatatcttt ggtgttgata atgctaataa aatgttattt tctcctttag
     5161 ataatgctaa aaatggcatg aagatttatc taaccgataa aaataaagtt tatacttatg
     5221 aaatacgtga agtcaaacgt gtgacaccgg atcgtgttga tgaagttgat gatagagatg
     5281 gggtcaatga aatcacatta gtaacctgtg aagaccttgc tgctacagaa cgtattattg
     5341 tcaaaggtga tttgaaagaa acaaaagatt attcacaaac atctgat
//
LOCUS       DAVLRT010000095         5180 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLRT010000095 DAVLRT010000000
VERSION     DAVLRT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.095"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            283..1828
                     /locus_tag="VXM85_001869"
     rRNA            283..1828
                     /locus_tag="VXM85_001869"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1880..1952
                     /locus_tag="VXM85_001870"
     tRNA            1880..1952
                     /locus_tag="VXM85_001870"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1913..1915,aa:Ala,seq:tgc)
     gene            2077..4978
                     /locus_tag="VXM85_001871"
     rRNA            2077..4978
                     /locus_tag="VXM85_001871"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5055..5170
                     /gene="rrf"
                     /locus_tag="VXM85_001872"
     rRNA            5055..5170
                     /gene="rrf"
                     /locus_tag="VXM85_001872"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1469 a         1046 c         1516 g         1149 t
ORIGIN      
        1 taacacgggt tcgaatcccg tacggactat ggtatgttgc ggttggaaca cttgatgaaa
       61 aaaagtttaa aaaaagctta aaaatcttca aaaaagtgtt gacaagtgaa agtggctgtg
      121 atatactaat atagttgtcg cttgagagaa gcaagtgaca aagacctttg aaaactgaac
      181 aagacgaacc aatgtgcagg gcgctacaac ataagttgta gtactgaaca atgaaaaaaa
      241 caataaatct gtcagtgaca gaaatgagta agaactcaaa cttttaatga gagtttgatc
      301 ctggctcagg acgaacgctg gcggcgtgcc taatacatgc aagtagaacg ctgaaggagg
      361 agcttgcttc tctggatgag ttgcgaacgg gtgagtaacg cgtaggtaac ctgcctggta
      421 gcgggggata actattggaa acgatagcta ataccgcata agagtggatg ttgcatgaca
      481 tttgcttaaa aggtgcactt gcatcactac cagatggacc tgcgttgtat tagctagttg
      541 gtggggtaac ggctcaccaa ggcgacgata catagccgac ctgagagggt gatcggccac
      601 actgggactg agacacggcc cagactccta cgggaggcag cagtagggaa tcttcggcaa
      661 tggacggaag tctgaccgag caacgccgcg tgagtgaaga aggttttcgg atcgtaaagc
      721 tctgttgtaa gagaagaacg agtgtgagag tggaaagttc acactgtgac ggtatcttac
      781 cagaaaggga cggctaacta cgtgccagca gccgcggtaa tacgtaggtc ccgagcgttg
      841 tccggattta ttgggcgtaa agcgagcgca ggcggttaga taagtctgaa gttaaaggct
      901 gtggcttaac catagtaggc tttggaaact gtttaacttg agtgcaagag gggagagtgg
      961 aattccatgt gtagcggtga aatgcgtaga tatatggagg aacaccggtg gcgaaagcgg
     1021 ctctctggct tgtaactgac gctgaggctc gaaagcgtgg ggagcaaaca ggattagata
     1081 ccctggtagt ccacgctgta aacgatgagt gctaggtgtt agaccctttc cggggtttag
     1141 tgccgtagct aacgcattaa gcactccgcc tggggagtac gaccgcaagg ttgaaactca
     1201 aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg
     1261 aagaacctta ccaggtcttg acatccctct gaccgctcta gagatagagt tttccttcgg
     1321 gacagaggtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa
     1381 gtcccgcaac gagcgcaacc cctattgtta gttgccatca ttcagttggg cactctagcg
     1441 agactgccgg taataaaccg gaggaaggtg gggatgacgt caaatcatca tgccccttat
     1501 gacctgggct acacacgtgc tacaatggct ggtacaacga gtcgcaagcc ggtgacggca
     1561 agctaatctc ttaaagccag tctcagttcg gattgtaggc tgcaactcgc ctacatgaag
     1621 tcggaatcgc tagtaatcgc ggatcagcac gccgcggtga atacgttccc gggccttgta
     1681 cacaccgccc gtcacaccac gagagtttgt aacacccgaa gtcggtgagg taaccgtaag
     1741 gagccagccg cctaaggtgg gatagatgat tggggtgaag tcgtaacaag gtagccgtat
     1801 cggaaggtgc ggctggatca cctcctttct aaggataagg aactgcgcat tggtcttgtt
     1861 tagtcttgag aggtcttgtg gggccttagc tcagctggga gagcgcctgc tttgcacgca
     1921 ggaggtcagc ggttcgatcc cgctaggctc cattggtgag agatcaccaa gtaatgcaca
     1981 ttgaaaattg aatatctata tcaaatagta acaagaaaat aaaccgaaaa cgctgtagta
     2041 ttaataagag tttatgactg aaaggtcaga aaataaggtt aagttaataa gggcgcacgg
     2101 tggatgcctt ggcactagga gccgacgaag gacgtgacaa acgacgatat gccttgggta
     2161 gctgtaagta agcgatgatc cagggatttc cgaatggggg aacccaacag gtaatacctg
     2221 ttacccacat ctgttaagga tgtgaggagg aagacgcagt gaactgaaac atctaagtag
     2281 ctgcaggaag agaaagcaaa agcgattgcc ttagtagcgg cgagcgaaac ggcagaaggg
     2341 caaaccgaag agtttactct tcggggttgt aggactgcaa tgtggactca aagattatag
     2401 aagaatgatt tgggaagatc agccaaagag agtaatagcc tcgtatttaa aatagtcttt
     2461 gtacctagca gtatcctgag tacggcggga cacgtgaaat cccgtcggaa tctgggagga
     2521 ccatctccca accctaaata ctccctagtg accgatagtg aaccagtacc gtgagggaaa
     2581 ggtgaaaagc accccgggag gggagtgaaa tagaacctga aaccgtgtgc ctacaacaag
     2641 ttcgagcccg ttaatgggtg agagcgtgcc ttttgtagaa tgaaccggcg agttacgtta
     2701 tgatgcgagg ttaagttgaa gagacggagc cgtagggaaa ccgagtctga atagggcgcc
     2761 ttagtatcat gacgtagacc cgaaaccatg tgacctaccc atgagcaggt tgaaggtgcg
     2821 gtaagacgca ctggaggacc gaaccagggc acgttgaaaa gtgcttggat gacttgtggg
     2881 tagcggagaa attccaaacg aacttggaga tagctggttc tctccgaaat agctttaggg
     2941 ctagcgtcga cattagagat tcttggaggt agagcactgt ttgggtgagg ggtccatccc
     3001 ggattaccaa tctcagataa actccgaatg ccaatgaatt atggtcggca gtcagactgc
     3061 gagtgctaag atccgtagtc gaaagggaaa cagcccagac caccagctaa ggtcccaaaa
     3121 taattgttaa gtggaaaagg atgtggggtt gcacagacaa ctaggatgtt agcttagaag
     3181 cagctattca ttcaaagagt gcgtaatagc tcactagtcg agtgaccctg cgccgaaaat
     3241 gtaccggggc taaaacaatt taccgaagct gtggatacct ttataggtat ggtaggagag
     3301 cgttctatgt gtgatgaagg tataccgtga ggagtgctgg aacgcataga agtgagaatg
     3361 ccggtatgag tagcgaaaga caggtgagaa tcctgtccac cgtaagacta aggtttccag
     3421 gggaaggctc gtccgccctg ggttagtcgg gacctaagga gagaccgaaa ggtgtatccg
     3481 atggacaaca ggttgatatt cctgtactag agtatgtagt gatggaggga cgcagtaggc
     3541 taactaaagc agacgattgg aagagtctgt ctaagcagtg aggtgtgaat tgagtcaaat
     3601 gcttaattct ataacattga gctgtgatgg ggagcgaagt ttagtagcga agttagtgac
     3661 gtcacactgc caagaaaagc ttctagcgtt taaacatact ctacccgtac cgcaaaccga
     3721 cacaggtagt cgaggcgagt agcctcaggt gagcgagaga actctcgtta aggaactcgg
     3781 caaaatgacc ccgtaacttc gggagaaggg gtgctgactt taagtcagcc gcagtgaata
     3841 ggcccaagca actgtttatc aaaaacacag ctctctgcta aatcgtaaga tgatgtatag
     3901 ggggtgacgc ctgcccggtg ctggaaggtt aagaggagtg cttagcgtaa gcgaaggtat
     3961 gaattgaagc cccagtaaac ggcggccgta actataacgg tcctaaggta gcgaaattcc
     4021 ttgtcgggta agttccgacc cgcacgaaag gcgtaatgat ttgggcactg tctcaacgag
     4081 agactcggtg aaattttagt acctgtgaag atgcaggtta cccgcgacag gacggaaaga
     4141 ccccatggag ctttactgca gtttgatatt gagtgtctgt accacatgta caggataggt
     4201 aggagtctaa gagatcggga cgccagtttc gaaggagacg ctgttgggat actacccttg
     4261 tgttatggcc actctaaccc agataggtga tccctatcgg agacagtgtc tgacgggcag
     4321 tttgactggg gcggtcgcct cctaaaaggt aacggaggcg cccaaaggtt ccctcagaat
     4381 ggttggaaat cattcgcaga gtgtaaaggt ataagggagc ttgactgcga gagctacaac
     4441 tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt ccgtatggaa gggccatcgc
     4501 tcaacggata aaagctaccc tggggataac aggcttatct cccccaagag ttcacatcga
     4561 cggggaggtt tggcacctcg atgtcggctc gtcgcatcct ggggctgtag tcggtcccaa
     4621 gggttgggct gttcgcccat taaagcggca cgcgagctgg gttcagaacg tcgtgagaca
     4681 gttcggtccc tatccgtcgc gggcgtagga aatttgagag gatctgctcc tagtacgaga
     4741 ggaccagagt ggacttaccg ctggtgtacc agttgtcttg ccaaaggcat cgctgggtag
     4801 ctatgtaggg aagggataaa cgctgaaagc atctaagtgt gaaacccacc tcaagatgag
     4861 atttcccatg attatatatc agtaagagcc ctgagagatg atcaggtaga taggttagaa
     4921 gtggaagtgt ggcgacacat gtagcggact aatactaata gctcgaggac ttatccaaag
     4981 taactgagaa tatgaaagcg aacggttttc ttaaattgaa tagatattca attttgagta
     5041 ggtattactc agagttaagt gacgatagcc taggagatac acctgtaccc atgccgaaca
     5101 cagaagttaa gccctagaac gccggaagta gttgggggtt gccccctgtg agatagggaa
     5161 gtcgcttagc tctagggagt
//
LOCUS       DAVLRT010000096         5169 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLRT010000096 DAVLRT010000000
VERSION     DAVLRT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.096"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..286)
                     /locus_tag="VXM85_001873"
     CDS             complement(<1..286)
                     /locus_tag="VXM85_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV1346394.1"
                     /translation="MDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARYREI
                     ILAEHDSCIKNDEAVRNFVTSVLLSAFVSALVSAMISLEIQTYKFVIPFII"
     gene            complement(297..2120)
                     /gene="dnaK"
                     /locus_tag="VXM85_001874"
     CDS             complement(297..2120)
                     /gene="dnaK"
                     /locus_tag="VXM85_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1346395.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2589..3113)
                     /gene="grpE"
                     /locus_tag="VXM85_001875"
     CDS             complement(2589..3113)
                     /gene="grpE"
                     /locus_tag="VXM85_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1346396.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(3140..4174)
                     /gene="hrcA"
                     /locus_tag="VXM85_001876"
     CDS             complement(3140..4174)
                     /gene="hrcA"
                     /locus_tag="VXM85_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1346397.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4338..4847)
                     /locus_tag="VXM85_001877"
     CDS             complement(4338..4847)
                     /locus_tag="VXM85_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1346398.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
BASE COUNT         1423 a         1136 c          963 g         1647 t
ORIGIN      
        1 caattatgaa cggtattaca aatttatatg tttgtatttc taatgatatc atagctgata
       61 ccagcgccga tacaaatgca gacaacaata ctgaggtaac aaaattcctt accgcttcat
      121 catttttgat acaactatca tgttcagcta atataatttc tctatatcta gctaaatatt
      181 ctattggaat attatcaata aaatcttttg atgttctata gttatcagct tgattacaaa
      241 aatcattata aacttttcgt gtatttttta gatactcttc atccaataca ctcgtcttac
      301 ttttccgtaa actctccgtc tacgacgtca tcgcctgcgt ttcctgttgc ttgtgcgcct
      361 tctgctcctt cttgagcttg ttgcgctgct gcggcttgtt cgtagagttt aacagcaagt
      421 ccttgagctt tttcgttcaa tgcttcaagt ttcgctttca tgtcgtccaa gttgttgtct
      481 tcttgagctt tcttaaggtc atcaagggca gcttgggcag cgtcacgttc tgcgtcgaag
      541 cctttacctt cagtttcctt gattgtcttt tcagtcgcaa agattgcttg gtctacttca
      601 ttacgaaggt ctacttcttc tttacgtttc ttatcggctt cagcgtttgc ttctgcatct
      661 ttcatcatgc ggtcgatttc ttcgtcagtc aaacctgagt tcgattggat gacaatagtt
      721 tgttcttttt gagttccaag gtctttggcc ttaacagaca cgataccgtt cttgtcgatg
      781 tcaaatgtta cttcgatttg aggaattcca cgaggtgcag ctgggatatc agtcaattgg
      841 aagcgtccaa gagtcttgtt atctgctgcc attgggcgtt caccttgaag aacgtggata
      901 tcaacggctg gttggttgtc tgctgctgtt gagaagactt gtgatttaga tgttgggatt
      961 gtagtgttgc gatcgataag ttttgtaaat actccaccca ttgtttcgat accaagtgac
     1021 aatggcgtta catcaagaag gacaacgtcc ttgacatcac cagtaatcac accaccttgg
     1081 atagccgcac ccatagcaac tacttcatca gggtttactg atttgtttgg ttctttacca
     1141 gtttcagctt taacagcttc aacaacggca gggatacgag ttgaaccacc aacaaggata
     1201 acttcgtcga tttctgacaa gctcaaacct gcatctgaaa gggcttgacg aactggaact
     1261 tttgtacgtt caacaaggtc acgagtcaaa tcgtcaaatt tcgcacgagt caaagtcatt
     1321 tccaagtgaa gaggtccagc ctcacctgca gtgataaatg gcaagctgat ttgtgttgaa
     1381 gttacaccag aaaggtcttt cttcgctttt tcagccgcat ctttcaaacg ttgcattgcc
     1441 atcttgtcag tagacaagtc gataccgttt tctttcttga attctgctac caagtggtca
     1501 atgatttttt ggtcaaagtc gtcaccacca agtttgttgt cccctgcagt tgacaatacg
     1561 tcgaagacac cgtcacccaa ttcaaggata gagacgtcga atgtaccacc accaaggtca
     1621 aataccaaga ttttttcttc tttgtcagtc ttgtccaaac cataagcaag agctgctgca
     1681 gttggttcgt taacaatacg ttctacttca agaccagcaa ttttaccagc gtcttttgtt
     1741 gcttgacgtt gagcgtcgtt gaagtaagct ggaactgtga taacagcttt ggttactttc
     1801 tcaccaaggt agtcttcagc gtagcctttc aagtattgaa ggatcatagc tgagatttct
     1861 tgtggagtgt attcttttcc atttgcagaa actttttcag aagttcccat cttagatttg
     1921 atagagataa ctgtatctgg gtttgtgact gcttgacgtt ttgcagcatc accaacgatg
     1981 atttctccgt ttttgaatga gactacagat ggagttgtgc ggtttccttc tgggtttgcg
     2041 atgattttgc tttcagttcc ttcaagaact gcaactgctg agtttgttgt acctaagtca
     2101 ataccgataa ttttagacat gtgtttttct ccttgttagt taattttcta tttttctttt
     2161 tgacactctt cttaagtggc gacgccgtca gagcttgact tcgtcaatct ctttgactaa
     2221 actttgagcc taaggtctca aaatttgcgc aaatagcgcc acggcgaaga gtatcgtctt
     2281 tggttcgctt cgctcactat tacaaagcca aacatatttt atctttcttc atagtttatt
     2341 gtcgtttcgg acaagttttc tattatgtaa attgcgacac gaggagtcga aatcgatttt
     2401 atttcgacga cgagttagta aggaagctag acaaacgcca tagcgattgc cgttttctga
     2461 cgagttgctt tagctaccgt cagaattgcc gaatcgaaca cgccctaagc tctgagtaaa
     2521 aaagatagat tttcttgtgt tcatcgaaca cttcgtcaat ctcctatttt tactgcgggc
     2581 tttgtatctt agttatacac cactaccatt gctgggcgta ggatgcggtc atggagtttg
     2641 tagccttttt gaaagacttg ggcgatggta tctactgggt gttcatcgtc tgctgggaga
     2701 gtttggatgg ccatatggta gttatggtca aattcgccat ccgctgcgat ttcttcaatt
     2761 ccttcttctt tcaaagcgtg aatcaagctt tcttgcacca tccccaagcc cttcttcaca
     2821 tcatctgtca aaccttcaac tgcaagtgca cgctcaaggt tgtcaagaga tggtaagatt
     2881 gcttttgcca agtcctggct acgataacgt tgcaagtttt gacgttcttc attggcacgg
     2941 cgttggatat tttgcatttc tgcatgagcg cgaagatatt tgttttcgaa ctcatctgca
     3001 cgttcatttg ccaagtccaa ctcagacttt tcaggagttg tttcttcagc tgtttccaca
     3061 acttcctctt cttgaacttc ttctacttct tcatttttta tatcttgggc catgttcgcc
     3121 tcctttaatg atttcaatct taatgtactt cataatgatt actgctgagg tagcggtaaa
     3181 aatctgtcaa cttcatggtc aaaacacggt tgaccacatt gatttgattg attagctgtt
     3241 ggtaatccag attaactgga ccgataatgg ctagaattcc aactccccga taaggaatga
     3301 ggaacttact actaatcacc gctaggtcag ctaaacagga ctcttgaccg tctgcaacac
     3361 gaacattttg catctgatcc tcacgcaacc cctcacgaat ctccaaggcc accttttgcg
     3421 gttggtcaaa gaactgatag gctgctagat tggcaaaatt caagagatgg accttgcccg
     3481 ccatcacaat gttttcgttg aacatttcct taaagatgtg ttcaaagaga tcgatgacat
     3541 tatccgttgt tgtaaagtaa cgctggataa tctgcggaat ctccgtccga atcttgtagt
     3601 gaatatctaa aacggtgtga ccgaggaaac gttcctgaat gatgctcttc agtttcagca
     3661 aatcctcctg caagaagttc cttggaatca gaaactgact agtaaccgtt cgcgactcgt
     3721 ctagggtaaa taccgccaag gctgtatgtt gccccaaaac aacgatatca aaggctgtca
     3781 accgttgcct gctcggctca acatccagtg ccactaccgt acagccactc aggtctgtta
     3841 gcaagttagc agcctcttgc agaatatcct ccaatttgaa gaattcctga tcaaaggctt
     3901 tgacaatctc atatacctca ttttcagcca gtcggtcaaa atccagtgag tgtttcacat
     3961 agtactgaaa accagcaaca cttggcatcc gaccgcttga agtatgagcc ttctcaagca
     4021 acccttgttt ttctagttcc gccatgtcat tacgaatggt tgcactgcta gagttaatag
     4081 actcttgcaa ggcttttgat ccgacaggtt cgtgcgtttt ggtaaagatg tcaataatca
     4141 gatttaaaat atcctgctga cgctctgtaa ccatctcatc actcctctct gtctgatcaa
     4201 ttagcactca atgcatccaa gtgctaactt atgattctat tatacaccct tagaagagaa
     4261 tgtcaagaca aaaactcaaa aaattagcac tcttttatca agagtgctaa aaatcaatct
     4321 gaagacctag aaccttgcta ttcatctact cggtatttcc tttttagtct gaaataacca
     4381 tgaattagat aagaaatcag aaaagcatat aaaacaaaaa taagaaagaa agacatagaa
     4441 agatcctctc taaacaaact cgtacaaaca acaagaccac ctaagaaagt agttgtacag
     4501 gaacgaagtc tagatcgcat aacttcccat ttgagcccct tactcatata gacttgatcc
     4561 tctggaagta aatcagaaga cgatttacga agaatcgaac aagaacctgc tcctatcaat
     4621 tcccaacctc tatctctaaa atcttgcagt tcatgcttat attttttaag aaatctagaa
     4681 tcatagatac ggtagatgac atcgtctggt tgacattggt caaaatagaa caaaccaaaa
     4741 cgactcgttc tatacctcca acctttcaaa tgcatctcat gtaaatattc ttcttccttg
     4801 tccaaatcaa caatagtgaa aatccgaaat tctactctgc tattcattgt cttaccccaa
     4861 aattagaaaa catgcctggc gttatttatt agataattct ttccgctttt gactcaatct
     4921 ccaaaaaata taagaaatct gaatcgcaaa aactatcaat aaaacccaat ctattatgaa
     4981 agtcaaaaac acttcccaac tgaaagaact acctccagtg acaaactttg agaaaaacgg
     5041 tagtagagct aaaaagagaa gcaaaatagg aagcatccgc attgttaaaa tccttttggc
     5101 ataaaaaaat ctttatttaa acgaaaatat tatggcaaaa tttacgccag tttttgaacg
     5161 gctgatgta
//
LOCUS       DAVLRT010000097         5058 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLRT010000097 DAVLRT010000000
VERSION     DAVLRT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.097"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(296..1024)
                     /locus_tag="VXM85_001878"
     CDS             complement(296..1024)
                     /locus_tag="VXM85_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1346399.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1182..2591
                     /locus_tag="VXM85_001879"
     CDS             1182..2591
                     /locus_tag="VXM85_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1346400.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2609..3904
                     /locus_tag="VXM85_001880"
     CDS             2609..3904
                     /locus_tag="VXM85_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1346401.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLALVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3909..4217
                     /locus_tag="VXM85_001881"
     CDS             3909..4217
                     /locus_tag="VXM85_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1346402.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4214..4522
                     /locus_tag="VXM85_001882"
     CDS             4214..4522
                     /locus_tag="VXM85_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1346403.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1705 a          856 c          925 g         1572 t
ORIGIN      
        1 atactatatg tgttacaaga aaagactcaa aaacttctcg tcagtgtaag aatgtttgaa
       61 acactattta aaactaaata actataagaa gtagatcagg tgaacaattt cagtacctac
      121 tctataagct atacgaagat atttttcaac atgatggata aaacgatcac tcttttaaag
      181 ctatcttatt tagaaaagag aaaccattta agcatagatg ggaccttctt gccctgtttt
      241 aaatttttct taaactagag tctgtaaaag attggcttca ttttcacatt ttctcctagg
      301 aggaatgtcg taatgcaaaa gaattaaatt caaataaatc atagcgatga cgactaatcg
      361 agtactcaaa cggagttccg ttatccaaat aagccacttg ttctacttca aataccggtt
      421 ccgttggaag gagctgcaag taatgttgct cattttctga actagaagaa gctcgcaaga
      481 tttttgtagc actggcaata tgcaatccta actttccttc aatgtaattg taaatcgatt
      541 tttgtaaaat atcttcagta atacctggaa taacatcggt actcatataa gtttgttcca
      601 gaacataagg tttaccgtca ataatacgca gacggtaaat attataaact ggactctgca
      661 aagcgacctg tagtttttct gctaaaaatt catttgcaaa ttctaatttg aagtgaataa
      721 tcctcgagtc aattgtagaa tgagcatctt cagatatttt tgtcagtccc cggatatctc
      781 tttctggaac gattaatttt ctttttgagc tgccacgaga gagaacaaag gttccctgcc
      841 cacgttttct aaaaattaag ccctcagaaa ctaacaagtc taaagctttt ttgatcgtca
      901 ttcgactaca ggcaaactgt ttagctaatg tcatttcatc aggcaattgt tcgttcatgg
      961 tatatttatt acaaagaatc ttttcacgaa tctcattgta aataaataaa tattttgtac
     1021 tcatttaatc tggttctctc tataatattc taatagtagt ataacaaagt tcaagaaatg
     1081 aggcaaacac aagacatgta tcaacaattt ttaaaaatgt ctagacaaat tttaaaatta
     1141 tgctatactt tatgtataga ataatagaaa ggaggttgag catgaaaaaa aagttagtat
     1201 ttcctaatct gttttggtgg ggagctgctt ctagcggacc tcagacagaa ggtcaatatg
     1261 gaaaagtaca tgaaaatgtg atggactact ggttcaaaac gcatccagaa gattttttcg
     1321 ataatgtcgg acctcttgta gccagtaact tttttcatac ttacaccgaa gatttccact
     1381 tgatgaagga aattggagtt aattctttcc gcacttccat ccaatggagt cgactcatca
     1441 agaatttaga gacaggtgag cctgatccaa aaggtattgc tttctacaat gccatcattg
     1501 aagaagctaa aaagaaccag atggatcttg tgatgaattt acatcatttt gatttaccag
     1561 tggaacttct tcaaaaatac ggtggttggg aaagcaaaca tgtagtggag ttattcgtga
     1621 agtttgccaa gactgctttc acatgctttg gagataaggt tcattactgg acaactttca
     1681 atgagccaat ggtcattcca gaagcaggat acttatatgc tttccattat ccaaatctaa
     1741 aaggaaaggg aaaagaggcc gtacaagtca tctataatct aaaccttgct agtgcaaaag
     1801 tgattcaact atatcgctca ttaggacttg atggaaagat tgggattatt ttaaacttga
     1861 cacctgctta tccaagaagt aattctccag aagacttaga agcaagtcga tttacagaag
     1921 acttctttaa caaagtcttc ttgaatccag ctgttaaagg aactttccca gaaagattgg
     1981 taaaacagct agagagagat ggcgtgttat ggagtcatac cgaaaaagag cttcaactga
     2041 tgaaatcaaa tacggttgat tttcttggag taaactacta ccatccaaaa cgtgttcaag
     2101 cacaagcaaa tcctgaggaa tatcagacgc cctggatgcc agaccaatac ttcaaagagt
     2161 atgaatggcc ggagcgtcgc atgaatccat atcgtggttg ggaaattttt ccgaaagcca
     2221 tttatgatat tgctatgatt gtgaaggaag aatatgataa tattccatgg tttatcagtg
     2281 aaaacggaat gggtgttgaa aacgaagcac ggtttatcga tgaaaatgga gttatcgatg
     2341 acgtgtatcg tattgaattt tatgaagaac atttaagatg gctacataaa gccattgaag
     2401 agggaagtca ctgttttgga taccacgctt ggaccgcatt tgattgctgg tcttggaata
     2461 atgcatataa gaatcgttac ggatttatct ccgttgattt agaaacgcaa aagagaacca
     2521 tcaagagctc aggaagatgg tatcgcaaag taagtgacaa taacggtttt gaagtagaaa
     2581 ttgaggagta aagagaggag atattcagat gaaaaactca aaatttatag atcaatttgc
     2641 cacctttgct ggtaaactag ggaaccaaat tcatttaaaa accctaagag atgcattcgt
     2701 aacagtaatg ccattatata ttttggcagg tttaatcgtt cttttgaaca acacggtatt
     2761 taagtggatt ttccaagggg atacattaac aaaattccaa tattggggaa taacaattgc
     2821 aaacggtact ttaagtattt caggtatgat tattgctgta atggttggtt atttcttagc
     2881 taaaaacaga gatttcgaaa acccgttagc agcatcaatg ctagcattag tttctttaat
     2941 tgtgatgatg ccaaatacag tttctgtagt tcctgacgga gcaaaagatg ctgtaaacat
     3001 ttcaggtgtt ctttcattca acaacacagg tacaggcgca atgttcgccg gggttatcgt
     3061 agcgattatt gcaacagaat tattcattga attatcaaac gttaaagctt tacaaatgaa
     3121 ccttggtgaa aatattccac cagctgttag tagatcattt agcgtattac ttccagtcat
     3181 gaccgtcatc tccttatttg gggttgtttc agcattatta ttcaatataa ctggaatgaa
     3241 cttaatctca atcattacaa tctttattca agaaccaatt cgtcatattg gtacaagctt
     3301 aatcggggtc attattattt actctttagg aaatatgtta tggctatttg gtattcacca
     3361 agcagttatt tacagtgcca tcctagaacc attactatta attaacatta ctgaaaacat
     3421 cactgcagca aataatggac aagccattcc acacatcatc aacctatcac aaatacaaac
     3481 attcgcttta atgggtggta gtggatctac attatgttta ttaatagcaa cattcttagt
     3541 gagtcgcaat gctgtctcta aaaacgtggc taaattatct tttggacctg gtatcttcaa
     3601 tatcaatgaa ccagtattat tcggttaccc aatcgtttat aacatttcat tagctattcc
     3661 atttatcaca gttccagtcc ttggtatttt aatcagctac ttagcaacag ttacaggatt
     3721 catgagtcct gcatttatac aagttccttg gactacacca gtattcttaa atgcatggtt
     3781 agcaacagca ggggacgtga gagcagttct agttcaattc atcatctttg cacttggagt
     3841 tcttctatac attccattta tcaaagttaa tgacaaagtt gttgaacaag aaatggaagg
     3901 ttaagattat gaaacaaatt ttattagtat gtaatgcggg aatgtcgaca agtatgcttg
     3961 ttaaaaaaat gcaacaaagt gcgactgaac gtgggattga aatctctatc caagcaaaat
     4021 caatgacaga agctaaaaag aatatccatg aagcagatgt cattctaatt gggccacaaa
     4081 ttcgttatga gttacttgct gtgaaagaaa ttgcaggaaa tatccctgta gataccattg
     4141 atatgcggga ttacggaatg atgaatggag caaaagttct ggaacaagca cttgcatgga
     4201 taggagaaat aagatgacag aagaaatgga atatatctgt tttcagctca ttgccaatag
     4261 tggagcagcc aaatcatcct ttattgaagc catccaacta gctaaggcag gaaatttaaa
     4321 agaagcgaaa attaaagttg aagaagcgga agactcctta gtagaagcgc acaagattca
     4381 ctctaatctc atacaaaaag aagctactgg agaaaaaata ggattctcct tgctatttat
     4441 gcatgcagaa gaccaaatgg cttcaacaga gattattcag ttgctatcta aagaattcat
     4501 cgatctctat caaaataaat aggaacactg tcattcccat ctgtctttat taatgacgac
     4561 tacattgtag gaacctccaa aagttggaca aaatgattga aaggattgga tcctgcatta
     4621 cacaagatcc aatccttttc ttttactcta gtttttataa tatgttgaat ctagaatagt
     4681 ccactgtagt ttctaaaaaa ttgctggaaa tggatgttaa gctccaattt atttgtttat
     4741 atcttatttc agtttactat actagcttat tattctagtg aaaaattgaa tgagccacta
     4801 tttatttaat atgtatgatg agggaatgac tcaaaaactt cttgtcgata taagaatatc
     4861 tgagatagta ttttaaaacg aaaggactat aaaagctcat ttccttcgca cttattttat
     4921 agagatctat cattatactc tttagaaatc aaaaagcaac acaccttgtc atcgttttat
     4981 cgtaaatctg ttaatgaggg ggctagtctt atctgcaatc tcaaatcagt gttttgagct
     5041 gaatggcact cgttttct
//
LOCUS       DAVLRT010000098         5005 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLRT010000098 DAVLRT010000000
VERSION     DAVLRT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.098"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            32..664
                     /locus_tag="VXM85_001883"
     CDS             32..664
                     /locus_tag="VXM85_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1346404.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            708..1364
                     /locus_tag="VXM85_001884"
     CDS             708..1364
                     /locus_tag="VXM85_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1346405.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            1383..2477
                     /locus_tag="VXM85_001885"
     CDS             1383..2477
                     /locus_tag="VXM85_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1346406.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGIAVANSLAAVKNGAGRVEETINGIRERAGNAALEEIAVALNIRQDYYQ
                     VETSIVLTETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQERVLVTVENRDTETILNAAGLDFDVLKASA
                     IAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            2489..3515
                     /gene="leuB"
                     /locus_tag="VXM85_001886"
                     /pseudo
     CDS             2489..3515
                     /gene="leuB"
                     /locus_tag="VXM85_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            3512..3781
                     /locus_tag="VXM85_001887"
     CDS             3512..3781
                     /locus_tag="VXM85_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1346407.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            3785..4144
                     /gene="leuD"
                     /locus_tag="VXM85_001888"
     CDS             3785..4144
                     /gene="leuD"
                     /locus_tag="VXM85_001888"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1346408.1"
                     /translation="MVGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            4306..4929
                     /locus_tag="VXM85_001889"
                     /pseudo
     CDS             4306..4929
                     /locus_tag="VXM85_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         1542 a          898 c         1153 g         1412 t
ORIGIN      
        1 taaatttgaa gtaatattca aggagaatca aatgatttac gaattttgtg ctgaaaatgt
       61 gactttactt gaaaaagcga tgcaggctgg agctcgtcgg attgaactct gtgataatct
      121 agcagttggt gggacaacac ccagctatgg agtgactaag gcagcggttg aactggcagc
      181 taactacgat acaaccatca tgaccatgat tcggccacgt ggtggtgact ttgtctataa
      241 tgacctagaa attgctatca tgctagaaga cattcgtttg actgctcagg ctggaagtca
      301 aggggttgta tttggaactt taactgctga taaaaagttg gataagccta atctggaaaa
      361 gttaattgct gcatcaaaag gaatggaaat tgtctttcac atggcctttg atgaactaag
      421 tgatgaagat caaccggaag ctattgactg gctcagtcaa gccggtgtca ctcgtatcct
      481 aactcgtgct ggtgtgtctg gcgactcctt agaaaaacgt tttgttcact atcacagaat
      541 tttggagtac gctaaaggta aaattgaaat tctaccaggt ggggggattg accttgaaaa
      601 ccgtcaaacc tttatcgacc aggtgggggt aacacaattg catggtacta aggttgtttt
      661 ttaaaaaata gaaaggaact gctagctttg ggtagcagtt ttcacttatg tttgaaattt
      721 ttaaatccta tcaatttaat caagaaaagg ctcatgatta tggttttata gaaaatagcg
      781 aagtctggac atatagttgc cagattttgc aaggtgactt tgtcatgact gtgtccatca
      841 ctgctgataa tgtgaacttt caagtctttg accaagagac tggtgacctc tatcctcacg
      901 ttcatatgga aagcatgagg ggaagttttg tcggaaatgt ccgtgaggct tgtctggaga
      961 ttctttacca gattcggaag gcttgttttg atgtgcaaga ttttatctgt catcagacta
     1021 agcgtatcat gactcaagtt caggaaaagt atggaaacca gttggagtat ctgtgggaaa
     1081 aatcgcctga tacagctgta ttgcgccatg aaggcaatca aaagtggtat gccgtcttga
     1141 tgaaaatctc ttggaataag ctggaaaagg gcagagaagg acaagtggaa gcagtcaacc
     1201 tcaagcatga ccaagtagct aatttgcttt cacaaaaggg gatttatcca gccttccata
     1261 tgagcaagcg ctactggatt agtgtgtccc ttgatgatac tttatcagat gaagaagtac
     1321 tggaattgat agaaaaaagt tggaacttaa cctctaaaaa atgaaatatt ttaataattt
     1381 tcatgaactt tcaattagct aaatattctt tactgaagaa attttcagaa aatataggat
     1441 ttaccacacc agaggaatat ggtgccatct tcaaatacct gattgagaat gtcaagacgg
     1501 atcgtcagat catctattcg cctcactgtc atgatgacct cggaatagca gtggcaaata
     1561 gccttgctgc tgtcaagaat ggtgcaggac gtgttgaaga gactatcaat ggtattaggg
     1621 agcgagctgg aaatgctgct ttggaagaaa ttgcagtggc tctcaatatt cgccaagatt
     1681 actaccaagt agaaaccagt attgtcctaa ctgagaccat caatacgtca gaaatggttt
     1741 ctcgcttctc tggtattcca gttcctaaaa acaaagccgt cgttggtggc aataccttct
     1801 cccacgaatc tggtattcac caagatggag tccttaaaaa tcctctcact tatgagatca
     1861 tcacacctga attggttggt gttaagattc cgcttggaaa attatctggt cgccatgctt
     1921 ttgttgagaa actgagagaa ttggccctag attttacaga agaggatatc aaaccactct
     1981 ttgctaagtt caaggcactg gtcgataaga agcaagaaat cacagatgca gatattcgag
     2041 ctttggtagc tggaaccatg gttgaaaatc cagaaggctt ccactttgat gatttacaac
     2101 ttcaaactca tgcagataat gacattgaag cgctcgttag cctagccaat atggatggtg
     2161 agaaagtcga atttaatgcg acagggcaag gttccgttga agcaatcttt aatgctatcg
     2221 ataagttctt taaccaatct gttcgtttgg tgtcctacac tatcgatgcg gtaacagatg
     2281 gaatcgatac ccaggaacgg gttttggtca ctgttgaaaa cagagataca gaaaccatct
     2341 tgaatgcagc agggcttgat tttgatgtgt tgaaggcttc tgctattgcc tatataaacg
     2401 ctaatacctt tgttcaaaaa gagaatgcag gtgagatggg acgcagtgtt tcttaccacg
     2461 atatgcctag tgtgtaaagg agaaggctat ggcaaagaaa atagtagctc tagcaggaga
     2521 cggaattggc ccagaaatca tggaggctgg tttagaagtt ctggaggctc tagctgaaaa
     2581 aacaggtttt gactatgaga tcgttcggag gtgcagatat tgatgcagca ggatctccct
     2641 tacctgatga aacccttaag acaagtaggg aagcagatgc tatcctacta gtagctatcg
     2701 gtagtcctca gtatgatgga gtagcggttc gccctgaaca aggcctgatg gctctccgta
     2761 aggaactcaa tctttacgct aatattcgtc ctgtaaaaat ctttgacagt ctcaagtatt
     2821 tgtcaccact caaaccggaa cgaatttctg gtgtagactt tgtcgtggtg cgtgaattga
     2881 caggcgagat ttactttgga gatcatattc ttgaagagcg caaagcgcgt gatatcaacg
     2941 actatagcta tgaggaagtg gagcggatta ttcgcaaagc ctttgaaatt gcaagaaatc
     3001 gcagaaaaat cgttactagt atcgataagc aaaatgttct agcgacctca aaactctggc
     3061 ggaaagtagc tgaggaagtc gcacaggatt tcccagatgt aaccttggaa caccagctgg
     3121 tagactcagc tgctatgctt atgattacca atcctgctaa gtttgatgtt attgtaacgg
     3181 agaatctttt tggagatatt ttatctgatg aatcaagcgt cttatctggt acacttgagg
     3241 ttatgccatc agccagtcat tctgaaaatg gaccaagtct ctatgaacct attcacggtt
     3301 cagcacctga tattgcaggt caaggaattg ccaatcctat ttccatgatt ttattagttg
     3361 tcatgatgtt gagagatagt ttcggacgtt atgaggatac agagcgtatc aaacgtgctg
     3421 ttgagacaag tctggcggca ggaattttaa cgagagatat aggaggtcag gcttcaacaa
     3481 aggaaatgat ggaagctatt attgcaaggt tatgaagtta gacgaaaaaa ttactctagt
     3541 ccttttgatt tggaatgtca tcattttctt gatttatggc attgacaaat tcaaggcaag
     3601 aagaagagct tggcgcatcc cagagaaaat cttactcatt ttagccctta cttgtggtgg
     3661 ttttggggcc tggttagctg gaatcacttt tcaccacaag actcgaaaat ggtactttaa
     3721 aacagtttgg tttcttggga tggtgaccac actagtagcc ttatatttta tttggaggta
     3781 atggatggta gggtcttcga gggaacacgc tgcttgggct ctagcggact atggttttaa
     3841 ggtcgtgatt gcaggatctt tcggtgacat tcattacaat aatgaactca ataatggcat
     3901 gttgccaatc gttcagccta gagaggttag agagaaacta gcccagctaa aaccaaccga
     3961 ccaggtaact gtggacttgg aacaacaaaa aatcatctca ccagttgaag aattcacctt
     4021 cgagatagat agcaagtgga aacataaact cctaaatagt ttggatgata tcggtattac
     4081 cttgcagtat gaagagttga ttgctgctta tgaaaaacaa cgaccagcct actggcagga
     4141 ttagaaaaaa tagaaaagga aatatagtaa actgaaataa gatgtaaaca aatgaattgg
     4201 agcttaacat ccatttccag caatttttta gaaactacag tggactattc tggattcaac
     4261 acattataaa attatgacaa aacacattca caagaaggct acgacatttt aaaaggtgag
     4321 ggcggatgta tcgtttgccc tactaaagtt ggttacatta tcatgaccag caacaaggca
     4381 ggacttgagc gtaagttcgc agccaaagaa cgtaagcgta acaaaccagg tgttgttctc
     4441 tgcggtagca tggatgaact tcgcgcttta gcgcaactca acccagaaat tgaagcattc
     4501 tactaaaaac attggatgaa gatattcttc ttggttgtat ccttccttgg aaaccagaag
     4561 cctttgaaaa actcaaagca tacggggatg gccgtgaaga acttatgact gatgtacgtg
     4621 gtactagctg ttttgttatc aagtttggaa aagcaggtga acaattggct gccaagcttt
     4681 gggaagaagg taaaatggtc tacgcctcat atgcttcaat gacaaaacga ttgaaactcg
     4741 ctatgagagc aaggtgtaat ggtgtctatg gtcgataagg acggcaaact catcccagaa
     4801 caaggaggag cacgttcaac ttcaccagca ccagttgtga tccgtaaagg gcttgacatt
     4861 gataaaatca tgatgcacct gtcagatact tttaactcat gggactactg tcaggttgag
     4921 tattattaag ataaaaaaag aagtctagtg ttatgagata ttaaagctcc taacactgga
     4981 ctttttgttt agaatttctt ttctt
//
LOCUS       DAVLRT010000099         4875 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLRT010000099 DAVLRT010000000
VERSION     DAVLRT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.099"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..183)
                     /locus_tag="VXM85_001890"
     CDS             complement(<1..183)
                     /locus_tag="VXM85_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1346409.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADSISKSQLSRFERGENEISL
                     STFFELLSNINVSIENF"
     gene            complement(196..969)
                     /locus_tag="VXM85_001891"
     CDS             complement(196..969)
                     /locus_tag="VXM85_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1346410.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAN
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            complement(1416..2171)
                     /locus_tag="VXM85_001892"
                     /pseudo
     CDS             complement(1416..2171)
                     /locus_tag="VXM85_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(2309..3931)
                     /gene="groL"
                     /locus_tag="VXM85_001893"
     CDS             complement(2309..3931)
                     /gene="groL"
                     /locus_tag="VXM85_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1346411.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(3947..4231)
                     /gene="groES"
                     /locus_tag="VXM85_001894"
     CDS             complement(3947..4231)
                     /gene="groES"
                     /locus_tag="VXM85_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1346412.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(4501..>4875)
                     /locus_tag="VXM85_001895"
     CDS             complement(4501..>4875)
                     /locus_tag="VXM85_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1346413.1"
                     /translation="IGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREADFVNM
                     VLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLLESRAQRA
                     MRENNAGQDLADLVLEEEELPF"
BASE COUNT         1389 a         1049 c          835 g         1602 t
ORIGIN      
        1 aaaattctct atcgaaacat tgatatttga taataattca aaaaatgttg aaagagaaat
       61 ttcattttct cccctttcaa atctagaaag ttgagatttt gatatagaat cagctgcagc
      121 atactctaaa gaatatccgt ttaattttct aaaatatcga aaagcttctc cgtaattttt
      181 cacactttca ttcccttaac tttcttttcg agcgataaac ataccatatt ctttaggaat
      241 gatcaacata ccattattaa actcactttc caatctttta cgtattattt cctcttctag
      301 tgaacctatt tctgaaattc ctttaaacga ttggatgtat ttaactaggt catctatact
      361 agttacctgt aactcatcat catagagtaa tgtatcgaca gagttaaaat acctactcag
      421 gtacctttta ccattttgta aagtaaacgt tctattttct aaatcttgat taacctcatc
      481 tttaaataaa tttgccaaat aatttacaac tccattttca ccaaatgtag cacagtaaaa
      541 aattcctccg gtttttaaga ccctattcac ctcagagaga gctttaggaa tatcattcac
      601 atgatgtaga agcatattag caataacaat atcaaaagtt tcattctcaa aagaaatctt
      661 ttgaatatcc attatctcat aattgacatt gtttctattt ccaatcactg acttcgttga
      721 ttttaccata tctttagaaa aatctgtaac aatcaactgc ttcattttat ctatagaatc
      781 actattactt ttccacaatt ctcctgtacc acatcccaat tctagaacct ttacttcatc
      841 agtgatttgg tagttagaaa atatccaatt attaaatccc aatttatttt ttgaatactt
      901 cttatgcaat tctactcgaa tatcaagatt atcatgactt ttatattgtt caattacttt
      961 atcaatcatt ttaaatgacc tccactctat aatttggcat tatatcacaa gaataaagat
     1021 agtgttaaat aagttgtata aatgcaaaaa aggaatattc cgttatagca gagttgtaac
     1081 acatttctat aaagaaattt attcctacga ctcagtttgg catgcttaac ttcctaactc
     1141 aaaaacaaag catcgattaa tttatccgtt cttcttttga aacagatttg aactgataat
     1201 tgctcgtttt aatttaagaa gctcttttga gcaaaacttc atttttttaa gagtccaaca
     1261 atttctgtct atcgcttttc tactagcaaa aaaaactacc ttgaggcaag ttttcattta
     1321 tatatctaat ttcgtaatag tatagtaaaa tgaaataagg acagtcaaat cgatttctaa
     1381 caatgtttta gaagtaaagg tgtactattc tagtttcaat ctactataaa tttgatatga
     1441 gaatagtctt tctcaacatc cttacccaac aagaatgctg tagcgtcctt cttaacttga
     1501 accaggtcaa tattctgggc ttgggctgca tagacgcagc cacaataaca ttgacgatag
     1561 atatcatact cctcacacat ctctactgaa cgtttgtagc cttgattttt cttgaaatcg
     1621 ttgggaagat agtgggtcgt gtaaattttt tgcacatcga ttccgatgct gttgatggtt
     1681 tgagaattct tatgaggact gatggtcaag gctgaaccaa agtagtcaaa gcccaagtcc
     1741 atagccactt gcgctgtttt atccagtcgg tagtcaaaac aaaccttgca acggtcgcca
     1801 ccttcgggct cctcctctag tcccctaact agttttcggt attaattggg ttcgtaggga
     1861 gcttctaggt actgaaccgt atttcctgtc cgctcattaa aatcactaac aaatttcttg
     1921 atgacataga cccgcttatg gtattctacc ttgggatgga tattagaatt ggcaaaatag
     1981 atggtcacat ctgcatactt ggtcaaatat tctagtgtat aggtactaca aggggcacag
     2041 caaacatgca cgagaatggt tggccgctgc tcatttttct cccatgcttg taccattttc
     2101 tgcatgacac ggtcataatt aatcttctga ttggggggca tcttgcacag aatttcttct
     2161 acatcgatca tgttcttctc ctttttctag tcttatttta tcatataagt taggagagct
     2221 caaatcaatt tagaagtgaa tattataaaa agaagggtta gtcattccct ccttttgtgt
     2281 tttttataag ttgttttcta tagaaagctt acatcatccc gcccatcatg cttggatcca
     2341 ttgctggagc tggggctact ggttctggtt tattggctac gactgcttct gttgtcaaaa
     2401 tcaagctggc tacagatgct gcattttgta gggctgaacg actcacttta actggatcaa
     2461 tgataccttg atcaatcatg ttaacccact cgccagttgc tgcgttgaat cctataccaa
     2521 gctcagcatt tttcaaacga tcgataacga tagatccttc aaatcctgca ttgtgagcaa
     2581 tttgacgaac aggttcttcc aaagcacgga gaacaatatt acgtcctgtt gcttcatctc
     2641 ctgtcaattc caaggtagca acagctggaa tcacattggc aagagctgtt ccaccacctg
     2701 caacaatacc ttcttcaaca gctgcacgag tagcgttgag ggcatcttca atgcggagtt
     2761 tcatttcttt caactcagtt tcagttgcgg ctccgacctt aataaccgct acaccacctg
     2821 acaatttggc caagcgttct tgcaattttt cacggtcaaa ttcagaagtt gtagtttcga
     2881 tttgagactt gataaccgca acacggtgag aaatcgcttc aggatttcct gcaccttcta
     2941 caataaccgt gctatctttg tccacggtca ctctcgctgc ttgaccaaga gcttcaattg
     3001 tcgcatcttt caactcaaga ccaaggtctt ctgtgataac tgttccgcct gttaagatgg
     3061 cgatatcttc aagcatagct ttgcgacggt caccaaaacc aggtgccttg actgctacta
     3121 cgttgaaggt tccacgaatc ttgttcaaaa caagagttgg aagagcctcg ccatccacat
     3181 catccgcaat aatcaagagt ggacgattgc tttggagaat gctttccaaa agtggcaaga
     3241 tttcttggat attggaaatt ttcttgtctg taatcaaaat gtacggattt tcaaggtcag
     3301 ccaccatttt ttcgctatct gtcaccatgt actgtgaaag gtaaccacgg tcaaactgca
     3361 ttccttctac gacttcaagc tctgtttcca taccacgtga ctcttcgatg gtgatgacac
     3421 cgtctttgcc aactttttcc attgcttcag agatgtactc accaactttt tcagaacgag
     3481 aagatacggc tgcaacttga gcgatagctt ctttattggc aacagggatg gcgttgtttt
     3541 tcaaagcttc aactgctgcg gcaactgctg tttcaatccc acgacgaata ccgattggat
     3601 ttgcacctgc tgtgacgttt ttgattcctt cacggacgat agcttgggtc aagactgttg
     3661 cagtcgtagt tccgtcacct gcgatatcat tggttttaga agctacttct gatactaact
     3721 tagcacccat attttcaaaa tggtcttcca attcgatttc tttggcaatg gtcacaccgt
     3781 cattggtaat caagggtgaa ccgaatgact tttcaagaac gacattgcga ccttttggtc
     3841 ccaaggttac tttaacagtg tctgcaagga tatcgacacc acgaaccatg gctgaacggg
     3901 catctgatga aaatttaatt tcttttgaca tacttacttt ctccttctat tcctcaatga
     3961 ttgccaagat gttagcttcg cctacgatga tgtacttttc atcgccatct ttgacatcaa
     4021 gacctgcgtg ggcttcaact aagacacgat ctccagtttt aacacttgga gcaaccaagt
     4081 caccgttcaa ggtacgaaca ccttgtccag tagccacaac ttgagctgtt ttggtttttt
     4141 cttgggctga gcctgcaagg acaaagcccc caacggtttg ttctttttct tctattttta
     4201 agaccacacg gtcccctaat ggtttcaaca tctgatttcc tccataatga gatagatatt
     4261 attagcactc tttgaacttg agtgctaatt cataattcta ttgtatcact tggtcagaaa
     4321 tagtcaagaa aaaagtctga ctttctcaag ataaaaagcc tgagaccaac tcagactttt
     4381 taatgtttat actcaatgaa aatcaaagag caaactagga agctagccgc aggctgctca
     4441 aagcattgct ttgatgttgt ggataaagct aacgtggttt gaagagattt tcgaagagta
     4501 ttaaaatggc aattcttcct cttccaagac caaatctgcc aaatcttggc ctgcattatt
     4561 ttcacgcatg gcacgttgag cacgactttc taagagttgg aatcctgtca caaggacttc
     4621 ggttacatag ttcatctgac catttttctc aaagcgacgg gtacgcaatt ctccatcaac
     4681 ggaaatgaga ctacctttgg ttgcgtagct tgccaaagtt tctgctagcc tgccccatag
     4741 gaccatattg acaaaatcag cttcacgttc accgttttgg tctttgtaac gacggttgac
     4801 agcgatagtt gctcgcgcta ccgacttgtc attgttggtt ttgtgcaatt ctggtgtaga
     4861 cgttaaacgc ccaat
//
LOCUS       DAVLRT010000100         4842 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLRT010000100 DAVLRT010000000
VERSION     DAVLRT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.100"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            221..1057
                     /locus_tag="VXM85_001896"
     CDS             221..1057
                     /locus_tag="VXM85_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1346414.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIIIGKGIAFGKRKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLKGYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            1093..1410
                     /locus_tag="VXM85_001897"
     CDS             1093..1410
                     /locus_tag="VXM85_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1346415.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            1410..3101
                     /locus_tag="VXM85_001898"
     CDS             1410..3101
                     /locus_tag="VXM85_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1346416.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            3310..4716
                     /gene="lacG"
                     /locus_tag="VXM85_001899"
     CDS             3310..4716
                     /gene="lacG"
                     /locus_tag="VXM85_001899"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1346417.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTDYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            4759..>4842
                     /locus_tag="VXM85_001900"
     CDS             4759..>4842
                     /locus_tag="VXM85_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 domain-containing protein"
                     /protein_id="HEV1346418.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLT"
BASE COUNT         1488 a          877 c          987 g         1490 t
ORIGIN      
        1 gatgaatttt agaacatagt taagttttaa aaaaagttgt atgtaaaggt ttacaaaaca
       61 accgacttgt gctatactta aatcacaagt taatacaagg tgagtgttac taagtaatat
      121 taggcatgat cacaggtgaa ttagaaatca gctgattttc tagttcattt gtggtcattt
      181 tttgtactca tataccttta agatataaaa ggaggttgac atgtatcgaa ttctaaatcc
      241 aatgaatcac aatgtctcgc ttgtcagaaa tgataaggga gaagaggtga ttataattgg
      301 taaggggatt gcattcggaa aaaggaaggg ggatttgatt gctgaaaatc aggttgagaa
      361 aatctttcgg atgaagaccg aagagtccag agaaaacttt atggctcttc tcaaagatgt
      421 tccgcttgat tttatcacag tgacctatga aatcattgat aagctatcaa agaaatatca
      481 ttatccgatt caagagtatc tctatgtaac cttgacagat catatttact gttcttatca
      541 agctctaact caaggaaggt acaaggatag taatctgccg gatatttccg ctaagtatcc
      601 tgtcgctttt caaatcgcaa atgaagcttt tgaaatttac cgtcagaagc tagcagatca
      661 ttttcctgag gacgagatta ttcggattgc ttatcatttc atcaatgccg aaggtgaaaa
      721 tgaagtggaa cttgtggagt cgattgataa gaggaaagaa attctcagga atgttgaaga
      781 agtgctaaag ggttatgcaa ttcaacgaac caaaaaaaat aatcatttct atgatcgctt
      841 tatgattcat ttgaattatt tcttggatta tttagacaga tctagagatg ataaccaatc
      901 acttctggat atggaagatc atattaaaca atcctatcca aaagccttcg agattggttc
      961 caagatctat gatgtgatta cgcaacatac gggtcttgat ttgtataaaa gtgaacgagt
     1021 ttatctagtt ctacatatcc aacgtttatt gtcataaaaa tttatttaaa actatataag
     1081 gagaattcta tcatgaatag agaagaagta acattgttag gttttgaaat cgtagcctat
     1141 gctggcgatg ctcgttcaaa actattggaa gccttgaagg ctgctgaagc tggtgatttt
     1201 gaaaaagcgg acgctctggt agaggaagct ggtagctgta ttgcagaggc tcaccacgcg
     1261 caaacaagtc tattgactaa ggaagcttct ggtgaggact tagcgtatag tgtgaccatg
     1321 atgcatggcc aagaccactt gatgacaact atcttgttaa aggatttgat gcaccatttg
     1381 atcgaactct ataagagagg agttaaataa tgaacaaact aattgcattt atcgagaaag
     1441 gaaagccttt ctttgagaaa ctatctcgta atatctatct tcgtgctatt cgtgatggtt
     1501 tcattgcagg tatgcctgtt attctcttct caagtatctt tatcttgatt gcctttgtac
     1561 caaactcatg gggctttaaa tggtctgatg aagttgtagc ctttctgatg aaaccttata
     1621 gctattctat gggtattctg gctctcttgg tagctggtac aacagctaag tcattgactg
     1681 actcagtaaa ccggagcatg gaaaaaacca atcaaatcaa ctatatgtca acgctgttag
     1741 cagcgattgt tggtttgttg atgttggcag ctgatcctat cgaaagtggt ctagctactg
     1801 gattcttggg gacaaaaggt ttgctttcag ccttccttgc tgccttcgtt actgtagcca
     1861 tctataaggt ttgtgttaag aacaacgtca ctattcgtat gcctgacgaa gttccaccaa
     1921 atatctcaca agtctttaaa gatgtgattc cattcactct atcagtggtt tctctttatg
     1981 ctcttgattt actagctcgt cattttgttg gttctagtgt ggcagaatca atcggtaaat
     2041 tcttcgcacc actcttctca gcagcagacg gataccttgg tattaccatt atctttggtg
     2101 cctttgcctt cttctggttt gtgggtattc atggtccatc tattgttgaa cctgctatcg
     2161 cagcgattac ctatgcaaat gctgaagtca acttgaacct tctccaacaa ggtatgcatg
     2221 ctgataaaat ccttacttca ggaactcaaa tgtttatcgt tactatggga ggtactggtg
     2281 cgacactggt tgttccattc atgttcatgt ggttaacaaa atcaaaacgt aaccgtgcaa
     2341 tcggacgtgc ttcagtagtt ccaactttct ttggtgtaaa tgaaccaatc ttgtttggtg
     2401 caccacttgt attaaatcca atcttcttca ttccatttat ctttgctcca atcgcaaacg
     2461 tatggatttt caaattcttt attgaaactc ttggaatgaa ctcattcact gctaatctac
     2521 catggacaac tccagctcca ctaggtctag ttcttggaac taacttccaa gtgctatcat
     2581 tcattcttgc tgcccttcta atcgtggttg acgttgtcat ttactatcca ttccttaagg
     2641 tctatgatga acaaattctt gaagaagaac gttcaggtaa gtctaatgat gaattgaaag
     2701 aaaaagttgc tgcaaacttc aacactgcaa aagcggatgc tattcttgaa aaagcgggtg
     2761 tagaagcagc acaaaataca atcactaaag aaacaaatgt cctcgttctc tgtgcaggtg
     2821 gaggaacaag tggtctcctt gcaaatgctt tgaataaggc agcagcagaa tacaatgtcc
     2881 ctgtgaaagc agcagcaggc ggctatggtg ctcaccgtga aatgttacca gagtttgatc
     2941 ttgttatcct tgcccctcaa gttgcttcaa actttgaaga tatgaaggca gaaacagata
     3001 agctcggtat taaactagcg aaaacagaag gcgctcaata catcaaatta actcgtgatg
     3061 gaaaaggtgc tcttgcattc gtacaagcgc aattcgatta aggctagaga ctctgaaata
     3121 gtctcctatc gttacggaaa tcgctatggc gaatttccta ttattaattc gtcggtaaaa
     3181 agatatcgtt tttacctcct catgtcacaa ttcggtgact tggtacaaga agtgagatgg
     3241 agaaggatgg ctcactgact cctctcctct cacttttact ttatttaaat caagaaatag
     3301 gtgaaaaaaa tgacaaaaac acttccaaaa gactttattt ttggtggcgc aacagctgcc
     3361 tatcaagcag aaggtgctac acatactgat ggtaaaggac cagtagcgtg ggataaatat
     3421 cttgaagata actactggta cactgcagaa ccagccagtg atttttacaa tcgatatcca
     3481 gttgacctca agctagcaga agagtatggt gtcaatggta ttcgaatttc tattgcttgg
     3541 tcacgtattt tcccgactga ttacggccaa gtaaatgcta aaggtgttga gttttatcat
     3601 aatttatttg cagagtgtca caaacgtcat gttgagcctt ttgtaactct tcatcacttt
     3661 gacacgccag aagctctcca ctcaaatgga gacttcttaa accgtgaaaa tatcgaacat
     3721 tttgtagact acgctgcctt ctgttttgaa gaatttccag aagtaaacta ttggacaacc
     3781 tttaatgaaa ttggaccaat cggtgatggt caatatttgg ttgggaaatt ccctccaggt
     3841 atccagtacg accttgccaa agtctttcaa tcacaccaca atatgatggt gtctcatgca
     3901 cgcgcggtaa aattgtacaa agagaaaggc tataaagggg aaattggtgt tgttcacgcc
     3961 ctgccaacta aatatcctct agatcctgaa aatccagcag atgttcgtgc agctgagttg
     4021 gaagatatca tccacaataa attcatctta gacgcaactt atctaggtcg ctattcagct
     4081 gaaaccatgg aaggtgtcaa ccatatctta tcagtcaatg gtggtagctt ggatcttcgt
     4141 gaagaagatt ttacagcatt agaagctgca aaagacttga atgatttcct aggaatcaac
     4201 tactatatga gtgactggat ggaagccttt gatggagaaa ctgaaattat ccataatggt
     4261 aaaggtgaaa aaggaagctc taagtatcaa atcaaaggtg ttggtcgtcg tgtagctcct
     4321 gactatgtac cacgcacgga ttgggattgg attatctacc ctcaaggttt gtatgaccaa
     4381 atcatgcgtg tgaagaaaga ttatcctaac tacaagaaga tttacatcac tgaaaatggt
     4441 ctcggctata aagatgagtt cgttgataac actgtttacg atgatggtcg tattgattac
     4501 gtgaaacaac acttggagat cttgtctgat gcgattgcag atggagctaa tgtaaaaggt
     4561 tacttcattt ggtcattaat ggatgtcttc tcatggtcaa acggttatga gaaacgttat
     4621 ggtctcttct acgtagattt tgaaactcaa gaacgttatc ctaagaaatc agctcactgg
     4681 tacaagaaag tagcggaaac tcagattata gactagtaga attagtcatt agatatagaa
     4741 ttttagtgag tcaaaaagat gttcaaagat tttatccaat ctatttatga aaaagtttat
     4801 attataaatt tcgaaaaatg ctctcaaata ccgtgtttga cg
//
LOCUS       DAVLRT010000101         4775 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLRT010000101 DAVLRT010000000
VERSION     DAVLRT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.101"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            85..1260
                     /gene="mutY"
                     /locus_tag="VXM85_001901"
     CDS             85..1260
                     /gene="mutY"
                     /locus_tag="VXM85_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1346419.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1315..2019
                     /locus_tag="VXM85_001902"
     CDS             1315..2019
                     /locus_tag="VXM85_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1346420.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2012..3361
                     /gene="vicK"
                     /locus_tag="VXM85_001903"
     CDS             2012..3361
                     /gene="vicK"
                     /locus_tag="VXM85_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1346421.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3363..4172
                     /locus_tag="VXM85_001904"
     CDS             3363..4172
                     /locus_tag="VXM85_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1346422.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4263..4505
                     /locus_tag="VXM85_001905"
     CDS             4263..4505
                     /locus_tag="VXM85_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1346423.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4507..4761
                     /locus_tag="VXM85_001906"
     CDS             4507..4761
                     /locus_tag="VXM85_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1346424.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
BASE COUNT         1463 a          830 c         1100 g         1382 t
ORIGIN      
        1 gatagaaaag ttatatacta tctattgaaa atattataaa tcagatacaa agacaggtga
       61 aaccttctgt gttatactag agatatgtta gatttgaaag aatacggtat cgtcatgtgg
      121 ccggaggaga aagtcatttc tttccgtgag aaacttctcg cttggtatga tgaaaacaaa
      181 agagatttgc cttggaggag aagtaaaaat ccttatcaca tctgggtatc tgaaatcatg
      241 cttcagcaga ccagggtgga tacagttatc ccttactacg aaagattcct ggactggttt
      301 ccaactgtcg aaagtctggc aactgcgcct gaggagagtt tactgaaagc ttgggagggc
      361 ttgggctatt attctcgagt tcgcaatatg caggctgcag cccagcagat tatgactgac
      421 tttggtggcc aatttccaaa tacctatgaa ggaatttcca gcttgaaagg gattggacct
      481 tacacagcag gagccatttc cagtattgct tttaacttgc ctgagccagc tgtagatggt
      541 aatgtcatgc gggtcttggc gcgtctgttt gaagtcaacc acgatattgg gattccaagt
      601 aatcgaaaaa tttttcaggc aatgatggag atcttgatta atccggatcg tccgggtgat
      661 tttaatcaag ccttgatgga cttagggtct gatattgagt ctcctgtaaa tcccagacct
      721 gaagaaagcc cagtcaagga ctttagtgcg gcatatcaga atggaaccat ggaccgttat
      781 ccaattaaat ctcctaagaa aaagccggtt cctatttatc ttaaagcctt agttgtgaaa
      841 aattctcaag gacaattttt acttgaaaaa aatgaaagcg aaaagctctt ggcaggtttt
      901 tggcatttcc cctttataga agttgataac ttttcgcaag aagagcagtt tgacctcttt
      961 catcaggttg cggaagaaag tgtgaacttt ggccccagtc cagaagagag tttccagcag
     1021 gactatgacc tagatgttga ttggcttgat gtttgttttg atacagtcca gcatgttttt
     1081 agtcatcgaa aatggcatgt acagattgta gcaggtcagg tgagtgactt ccatgatttt
     1141 tcagataggg aagttcgttg gctttcacca gaagaattca agaattaccc acttgccaaa
     1201 ccccaacaaa aaatctggca ggcttatgca caagccaact tagacagtag ccaagactag
     1261 ctattgtgtc ttctgactat tttttggtat aatagtagag aaaaaggtga acatatgaaa
     1321 aaaatactaa ttgtagatga tgagaaacca atctcggata ttatcaagtt taatatgacc
     1381 aaggaaggtt acgaagttgt aactgctttt aatggtcgtg aagcgctaga gcaatttgaa
     1441 gcagagcaac cagatattat tattctggat ttgatgcttc cagaaattga tggtttagaa
     1501 gttgctaaga ccattcgtaa gacaagcagt gtgcccattc ttatgctttc agccaaagat
     1561 agtgaatttg ataaggttat cggtttggaa cttggggcag atgactatgt aacgaaaccc
     1621 ttctccaatc gtgagttgca ggcgcgtgtt aaagctcttc tgcgtcgttc tcaacctatg
     1681 ccagtagatg gtcaggaagc agatagtaaa cctcaaccta tccaaattgg ggatttagaa
     1741 attgttccag acgcctacgt ggctaaaaaa tatggcgaag aactagactt aacccatcgt
     1801 gaatttgagc ttttgtatca tttagcatcg catacaggtc aagtcatcac gcgcgaacac
     1861 ttgcttgaga ctgtctgggg ttatgactat tttggtgatg tccgtacagt tgatgtgact
     1921 gtacgacgtc tgcgtgagaa gattgaagat acgcccagcc gaccagagta tatcttgacg
     1981 cgccgtggag tagggtatta catgagaaat aatgcttgat ttactgaaac aaaccatttt
     2041 taccagagat tttatcttta tcctgatttt gttaggtttc atccttgttg tgaccctctt
     2101 attactggaa aatagacgtg ataatattca gttgaagcaa atcaatcaaa aggttaaaga
     2161 tttgattgca ggagattatt ccaaggttct tgatatgcaa ggtgggtctg aaatcaccaa
     2221 tattaccaat aatttgaatg acttgtcgga ggttattcgt ctcactcagg aaaatctaga
     2281 acaagagagt aagaggctaa atagtattct gttttatatg acagatgggg ttcttgcgac
     2341 caaccgtcgg ggtcagatta tcatgattaa cgatacagcc aagaagcaac tggggttggt
     2401 taaggaagat gttctgaata gaagcatttt ggaattgctc aagatagaag aaaactatga
     2461 attgcgtgat ttgattaccc aaagtccaga attgttgcta gattcccaag atatcaatgg
     2521 cgaatatttg aaccttcgag ttcgctttgc cttgatacgt cgagagtctg gctttatttc
     2581 aggtttggtg gctgttttgc atgatacgac ggagcaggag aaggaagaac gcgaacgaag
     2641 actctttgtt tccaatgtta gccatgagtt acggactcct ctgactagcg taaaatccta
     2701 tcttgaagcc ttggatgagg gggctttgtg tgaaactgta gcaccagact ttatcaaggt
     2761 ttctcttgat gagaccaacc gtatgatgcg catggtgacg gatctcctcc atctttcacg
     2821 tattgataat gctaccagtc acctagatgt ggaactgatt aacttcactg cttttattac
     2881 ctttatcctc aatcgttttg acaagatgaa aggacaggaa aaggagaaaa aatatgagtt
     2941 ggtgagagat tatcccatca attctatctg gatggaaatt gatacagata agatgacgca
     3001 ggttgtcgac aatattttaa ataatgctat taagtattcg ccagatgggg gtaaaatcac
     3061 tgtcagaatg aagacaactg aagaccagat gattttatcc atttctgacc acggtttggg
     3121 gattcctaag caggatttac cacgtatctt tgaccgtttc tatcgtgtgg atcgtgctag
     3181 aagtcgtgcg caaggtggta caggtctagg actgtctatc gctaaagaaa ttatcaaaca
     3241 acataagggc tttatttggg ccaagagtga atacggcaag ggttcaacct ttaccattgt
     3301 gctcccttat gataaggatg cagtgaaaga agaagtatgg gaggatgaag tagaagacta
     3361 gaatgagtga aataggcttt aaatacagta ttttagcgtc gggttccagt ggaaattctt
     3421 tttatctgga aacctcaaaa aagaagcttt tagtagatgc aggcttgtct ggcaagaaaa
     3481 ttaccagtct gctagctgaa attaaccgta agccagaaga cctggatgcc atcttgatta
     3541 cccatgagca ttcagatcat atccatggag taggcgtttt ggctcgcaag tatggtatgg
     3601 atctttatgc caatgaaaag acctggcaag ctatggaaaa tagtaaatat cttggcaagg
     3661 tggattcttc gcaaaagcat atttttgaaa tgggtaaaac caaaaccttt ggagatatcg
     3721 acatcgagag ttttggtgta agccatgatg cagtcgcacc gcagttctat cgctttatga
     3781 aggatgataa gagttttgtc cttttgacag atacaggtta tgtcagtgac cgtatggcgg
     3841 gcattgtcga aaatgcggat ggctatctta tcgaggccaa ccatgatgta gagattttgc
     3901 gatcaggttc ttacgcttgg cgactcaaac aacgaatcct atctgacctt ggtcaccttt
     3961 ctaacgagga cggtgctgaa gctatgattc ggacgctagg aaatcgtact aagaagattt
     4021 accttgggca tttatctaag gaaaataata tcaaggaact ggctcatatg accatggtca
     4081 atcagctggc tcaagctgat ctgggagtcg gagtagactt taaggtttat gatacctcac
     4141 cagataccgc aacaccattg acagagatat agaaagaacg ctgagaaggt gttcttttta
     4201 tattgactga acacctaaaa agtaatacaa tggtgttacc attaaaaaag ggagcacaaa
     4261 agatgactac tattacatta aaagtttctg aagctgataa aacatttatg aaagcaatgg
     4321 ctaagtttga aggagtttcc ctttcggaac ttattcgcac caaaactctt gaagccctag
     4381 aagatgaata cgatgctcgt gtggcagatt tagcctatca agagtattta gaagacttgg
     4441 aaaaaggagt tgaacccatt acttgggaag aaatgatgca tgatttaggc ttgaaggatg
     4501 aataatttgt ataaattagt tccaacaaga cgttttatca agcaattgaa aaaattggac
     4561 cgttatacgc agaagctaat tacaaactat ttacaaacca atgttttgga aggtccaaga
     4621 cgacacggaa aggctttggt tggtaatcgc gttggtcaat ggcgctatag aattggtaat
     4681 tatcgagtta tcgtacaaat tgtagatgat gaattagtcg ttgctactct agaagttggt
     4741 catcggagag atatttattg aattaaagct gagta
//
LOCUS       DAVLRT010000102         4660 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLRT010000102 DAVLRT010000000
VERSION     DAVLRT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.102"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(298..1524)
                     /locus_tag="VXM85_001907"
     CDS             complement(298..1524)
                     /locus_tag="VXM85_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1346425.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISEILPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1596..2261)
                     /locus_tag="VXM85_001908"
     CDS             complement(1596..2261)
                     /locus_tag="VXM85_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1346426.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2273..2950)
                     /locus_tag="VXM85_001909"
     CDS             complement(2273..2950)
                     /locus_tag="VXM85_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1346427.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            complement(2972..3205)
                     /locus_tag="VXM85_001910"
     CDS             complement(2972..3205)
                     /locus_tag="VXM85_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346428.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            complement(3491..4354)
                     /locus_tag="VXM85_001911"
     CDS             complement(3491..4354)
                     /locus_tag="VXM85_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1346429.1"
                     /translation="MIERMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRFGRKVVDRFAHQDIVGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKSGFLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIVLFE
                     LRLQQYKKLID"
BASE COUNT         1646 a          912 c          821 g         1281 t
ORIGIN      
        1 aaccatacct acggcaaagt gaagctgacg tggtttgaag agagtttcga agagtataag
       61 tagactgaaa agaatccagc cacagcatgg actattatat agcagattga aataagatga
      121 gaacaaatcg attgggaaag taaaattaat ttctataaat tttttagcaa ttgtttcgta
      181 ctattttaga ttcagtctat tataacacat tcagaaaaga gaaaaaagtc tgttgatttt
      241 gaccatcata aaaagactgg caatccagtc tcaaacatat attatagaaa ttctccacta
      301 aatactttca cgaatattca gaagcataac aaaggcaact agaagaaata gcaataaaac
      361 aaagctaact gccagagttc caaagctagt agcaatggtt accaaagcta ttgtaaataa
      421 gctaggtaaa acaaccgtaa tggcaccgat agaggattga actgctccca ttgactcctc
      481 aggtatttgt ttaaaaacga gttcttgcaa tctaggagag agaacacctg cgaaaaaggc
      541 atccaaggta ctaaagatga gaatccagtc aaaacgaact gtggcaaatc ctactagaag
      601 aagcaactgg atgacaagtg aggcatagag agctgttttt atggaaatgg tatgttgcag
      661 atagccactt acaaggcttc cgacaatcag ggctgacagt tcaaaagtgg atagcaaggc
      721 aagagattga cctgtttgta aattcaaaaa gggttgattc ctcaaaaata gagtagagac
      781 aggaactgta acatttatca ctgcttgact agtagagata ataaacaaaa ccaagagcac
      841 cttattcata ttccatatca atttcgatga ttggagcaaa tgctggcaaa aggattttac
      901 agagagtcct tcttgatagt taatcgtttt ttctactttc aagaggtcag tttttatgaa
      961 aaggatacct aaaaatgcga ttaaaaaggt aagagcgttc agcaaggaaa tgaactggat
     1021 ggagagaatc cctagcaaga ctcctcctag aatattactg attgttttca ctaaactaac
     1081 agttgactgt ttaaatccaa tagcttctgc cagatggtct tgcccaataa ttctaatgaa
     1141 aatcggagtg agcatggctc ctgaaaagta actcaatgtg tcagacaaga ggttaatcag
     1201 acaaataaat gctactagca acaaggagaa agactgccct gaaagtgata aagaaacgat
     1261 agagtaaagc aaaaattttg caaaactaat gactgtgtat ttcaagacac gatgatgttg
     1321 aaaatctgcc aaaactccca gaaagatttg taaaatttgg ggaaggattt ctgaaatcgt
     1381 aatgagtaaa atcgccaaag gtgcaaaaga cgcatctgcc acataattca gaaaggccag
     1441 ataaaaaatc gtatccccaa gcgttgaaat ccactggttg atagttaatt gcctaaaatc
     1501 tctattttga agaaatactt tcatcacaac tccttcttaa gttcaaatgg gaatctttcc
     1561 ccagaaatag accgcgatac tactaacaac caaaattaca gtaacatcaa aagctgacca
     1621 atgccgttgt agactatatg cagcccaata ggccaataaa tggactttgt cattctaaat
     1681 aagactgcaa atataagtcc gccacccata tagacgaaaa agtctgtcaa gacccaaccg
     1741 tgattactaa tgtgcgagac cccaaataaa atagcagaac caagtacatc tagcccccat
     1801 ttctttcctt tttccagagc tgtcatcacc aatccccgat ggattatctc ttcaaaaatg
     1861 ggacctgcaa tcacaggata gaaaaaatac atcaaaaatg ctgtagcccc tgtaaaagtc
     1921 ggagcagcat gttgataaga aatttcattt cgagtaggtg ggaaaagaaa aaaggtaacg
     1981 aaattccaaa caacaaaagc aagcagagct aggaaggaat agaaaagata ggatccttta
     2041 aactttctac tattgatttt ctgccatttc cccgaccaaa tcatagcaat aagagcaaat
     2101 aaaaccacaa gaaaattcaa catcatatcc gacagataat aggcaaagtc agatagccca
     2161 gtaacaaggt cgctgcgtaa aactagaaca ctgaacttct ggtcagcaat aactagtaga
     2221 aaaactataa taaagtagcg gtgtgagatt atctttttca tatatcacct ttctaatatc
     2281 caaataccaa taaagtaaca atgagtaaga aactattcca tgaagcatgc agagctatag
     2341 cccaatagat ggatcgggtg tagcgaaaca tcatacaaaa tatcaagccc attccaaaat
     2401 actttatgaa atctgtcgtt atccaaccat actgcaaaac atgcatagcg ccaaatatgg
     2461 cagcggaaac aagaacatca agatagtatc tcttaacttt agataaactt gtcatcaaaa
     2521 gaccacgaca aacaacctct tctgatacag gtgcgataat actagtataa agtattcgcg
     2581 taacaaaata gctaattcct gttaaattgg tggctacttc tacgactgta cttccattct
     2641 gggtacgagg aaagatatag gttgttagat ttgcccacac gaacaataag aaaaaagaaa
     2701 gaaggaaaac acccaggtaa gaccaacgaa actggaaacg accacactct ttccaatgtt
     2761 cacttttgac aaaagcaatt gtagctatag ttcccagaat aagtaccaat aaaacttgga
     2821 acacatagta catattatca gacaaagcaa ccataaaatc taagtctgat gtgacattaa
     2881 aaatgaggta ataagtcaaa atcaacaagc cagttgctag gtgaaatttc acttctttca
     2941 ttttcttcat cctattatct cctataagag cctatcttct acggcggcca aacaatccat
     3001 ctgctaaatc tatagtccaa tcaaaagctc cacgattagg actcatccct tgattgcccc
     3061 aaccagggta aattcctggg acgccccaac cagatatacc acttcttcca ccacctccca
     3121 tagaatttac gaggttgcct cctctaacat cttgcaactc agcttctgtc aattccattg
     3181 tttctgcaaa ttgtaaattt aacatctttt acactccttc aattatcttc atttgtaaac
     3241 cacttctgcg acctaggatt tgcttcaagt gctttacaag tacagtataa cacgaaaatt
     3301 ggcttatttt agaaaatcgc atatttgata ttttttctta tagaaatttc agatttgcga
     3361 ttttggtgaa tttgattact tctctggtat aataaagtta ctactaatga ggagtggaga
     3421 aatatgaaga aacaaatttt aacattattg aaaatcgttg ctgagattat tattattttg
     3481 ccatttctaa ctaatcgata agctttttgt attgctgcaa acgcaattca aaaaggacta
     3541 ttaattgtgg attttctaat acttgcagag attggataaa gtgttcaatc tctttttggt
     3601 tgcttccctt ggtttgaaga aaaatactca ttttctttaa aaactgccac aacactttct
     3661 caaaaacatc atacggacgg agcatactct ccaactcggc ttcaaagatt gggatgtaag
     3721 agaaaagttt gcgctccatg agctctgata taatattcag aaagccactt ttcatataca
     3781 gtctattatg taccaactct ttaaattcct tagatttttc aagcaaagag gttgataaaa
     3841 aaatcaaatc ttggttgctc aagaagggca tggtattgca aaagagatag agttcaaacc
     3901 aggtccaaga ctcgatagca tagagatagg tggttaaaaa ctcgctatcc tcctctgcta
     3961 gcggatagct tttatctaag gaatggatgg catctttaat cacgatggca ttcaaacgac
     4021 gataggtctc tgccatctgt tcttgctcga cttcctccaa tagttgttct aagccaacta
     4081 tatcttgatg ggcaaagcga tccacaacct ttctaccaaa tcgcatatgt ggagattctt
     4141 gatagttgtt gagcttgtgc ccaaactcat caaaattcat attgatccct tggatggcta
     4201 atatgagctt atctgcagac aacatagact gccctagttc aaacttggac aactgagagg
     4261 ctgttagacc agcacaagcc acatctgact gcttgagctt tctagccagg cgcagttctt
     4321 tgtaaaattc tcccaattcc attctctcga tcatctcacc acctcctatt ttttatatag
     4381 tatatcattg tttttatagt tttgtcaaaa cattctgaca ctttggtgat tgaaaaagcc
     4441 agccttaagc tgactctaaa aggaccgagc cttgtacaat acagaactca atcctcgttc
     4501 taaatagaat gtctaacttt tttgggcagt acacgagctt ggggaacagc cactggaacg
     4561 atgaggtgtg agctcaaaat atcctccagt tatgtttttc ctaatagtat accggaagag
     4621 tgaaaggatt ttataatgga gcggttacaa agaacctact
//
LOCUS       DAVLRT010000103         4628 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLRT010000103 DAVLRT010000000
VERSION     DAVLRT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.103"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..748
                     /locus_tag="VXM85_001912"
     CDS             <1..748
                     /locus_tag="VXM85_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1346430.1"
                     /translation="IPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGET
                     VAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLS
                     YGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMI
                     LLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKIL
                     VMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            772..2511
                     /locus_tag="VXM85_001913"
     CDS             772..2511
                     /locus_tag="VXM85_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1346431.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDNNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            2562..3329
                     /locus_tag="VXM85_001914"
     CDS             2562..3329
                     /locus_tag="VXM85_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEV1346432.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            3331..3963
                     /locus_tag="VXM85_001915"
     CDS             3331..3963
                     /locus_tag="VXM85_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346433.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(4474..>4628)
                     /locus_tag="VXM85_001916"
     CDS             complement(4474..>4628)
                     /locus_tag="VXM85_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEV1346434.1"
                     /translation="RTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRKK
                     ASLMMH"
BASE COUNT         1609 a          676 c          880 g         1463 t
ORIGIN      
        1 aattccagaa agagtcagtc aagatataaa atttgataaa aaaatagaat taaaagatat
       61 ttggtataaa tatggattat ttgatgatta tgttttgaaa ggaataaatg ttactattaa
      121 aaaaggagaa actgttgcta ttgttggaga atcaggttca ggtaagagta cattagctaa
      181 aattttatta ggtttattag aacctaatat tggttcaata gaagttgatg gagtagaaaa
      241 agaagaaatt ggtcaaacat tgtatagaaa gatttttgga gcagtgttac aaaattcaac
      301 cctaagttat ggtaccttaa gagagaattt gacatttgga cactttgttt cagatgaaga
      361 attaatgaca aatctaaatt caattggtct tagcaatgta gttaaatctt tacctcttgg
      421 attagagaca atcatcgctg aagaaggtaa taacttttct ggagggcagc agcaaatgat
      481 acttttagct cgttgtcttt tgtcgaaacc ttcggtagtt gttttggacg aagcaacaag
      541 tagtttagat aatttatctc aacaaattac aacttcttac ttaagtgaaa tcggtaccac
      601 taagatttta attgcccatc gactagatac tatcaagtct gcagataaga tcttagtaat
      661 gcataatggt gaaattgtag agattgggac ccatagagaa cttcttgaac taggaggcat
      721 ttataagcaa ttgtattcaa ataattagtt tttgattaaa agggtaaatt tatgaagatt
      781 atgaaaaaaa aatattggac tttagcgata ttattctttt gtttgttcaa taattctgtt
      841 actgctcaag aaatacctaa aaatcttgat ggcaatataa ctcacactca gactagcgaa
      901 agtttttctg aatctgatga aaaacaggtt gactattcta ataaaaatca agaagaagta
      961 gaccaaaata aatttcgtat tcaaatcgat aagacagaat tatttgtaac aacagataaa
     1021 catttagaaa aaaactgttg taaattggaa cttgaaccac aaataaataa cgatattgtt
     1081 aactctgaaa gtaataattt actaggcgaa gataatttag ataataaaat taaggaaaat
     1141 gtttctcatc tagataatag aggaggaaat atagagcatg acaaagataa cttagaatcg
     1201 tcgattgtaa gaaaatatga atgggatata gataaagtta ctggtggagg cgaaagttat
     1261 aaattatatt ctaaaagtaa ttctaaagtt tcaattgcta ttttagattc aggagtcgat
     1321 ttacaaaata ctggattact gaaaaatctt tcaaatcact caaaaaacta tgtccccaat
     1381 aaaggatatt taggaaaaga ggagggagag gaaggaataa tatcagatat tcaagataga
     1441 ttaggtcatg gtacggctgt tgtagctcaa attgtagggg ataacaatat taatggagta
     1501 aatcctcacg ttaatattaa cgtctataga atatttggta agtcgtcagc tagtccagat
     1561 tggattgtaa aagcaatttt tgatgctgta gatgatggca atgatattat caatcttagt
     1621 actggacaat atttaatgat tgatggagaa tatgaggacg gaacaaatga ttttgaaaca
     1681 tttttgaagt ataaaaaggc tattgattac gcgaatcaaa aaggagtaat tatagtagct
     1741 gcattaggga atgactccct aaatgtatca aatcagtcag atttattgaa acttattagt
     1801 tcacgcaaaa aagtaagaaa accaggatta gtagttgatg ttccaagtta tttctcatct
     1861 acaatttcgg tcggaggcat agatcgctta ggtaatttat cagattttag caataaaggg
     1921 gattctgatg caatatatgc gcctgcaggc tcaacattat ctctttcaga attaggactt
     1981 aataacttta ttaatgcaga aaaatataaa gaagattgga ttttttcggc aacactagga
     2041 ggatatacgt atctttatgg aaactcattt gctgctccta aagtttctgg tgcgattgca
     2101 atgattattg ataaatacaa attaaaagat cagccctata attatatgtt tgtaaaaaaa
     2161 attctggaag aaacattacc agtaaaaaat ggtataaaag tgttaaatat accaaacgta
     2221 ttgagatatg atttgaatat gttacaatta gaatataaaa atgaacaaag ttgggatagt
     2281 ttcatagata atgttaattt aattgagttg gaagagagaa ttcaaactac tattggaatt
     2341 aaacaaataa acacacacaa tattattact attgcccgag aagggtactc tcaaaattat
     2401 ttacctaaca cttcagaaaa tacatataat tcattacaag tcagtttagt tggagtatta
     2461 ctacttttta taagtatggt aaatatttta tgggctaaaa aaagtaaatg aaaataaaat
     2521 ttggagccct ctgaaaaagt aagtcctaca gttcaactaa aatgagtcaa aagatgaatc
     2581 accttgatgt aggggagttt gtcttattgc tgcctgaaca cctccgttca gaggaagaac
     2641 attataaatc tgtttttgaa gacgacttaa ccagtcgcat atctagtcaa gatgaacgac
     2701 agcaaatgac tgctacggta ggttatttag aatcaggtca ggatcgtttt gtgtataata
     2761 cgacccctat ttcttaccag cagtttttga aagatccaat catcattgtt ataacacccc
     2821 aatcaactgg tccacagtcc attttgtttt ggatagacgc agtacagaac tacgttctct
     2881 ttaatcaatt gtctgatgcc caggagctta tccagagaca aggcattgaa aattgggtct
     2941 cagaaatgca aacaggttac cacaactaca tcacattatt ggataatatc cagagggaac
     3001 gttgggtaat gctagcagga gctgtgcttg ggattgcaac ttcaatcttg ttgtttaaca
     3061 ctatgaatag gctctacttt gaagaattta gacgtgccat ttttatcaaa cgcattgcag
     3121 gtctcaggtt cttagaaatc catcgcactt atctctttgc tcaactgggt gtgtttttac
     3181 tgggatttgt tgcgagtgta tttcttcagg tagagatagg agttgctttc ttagtcttgt
     3241 tactctttac tggtctatct cttttacagt tacatgtcca aatgcagaaa gaaaacaaga
     3301 tgtccattct tgttttgaag ggaggttaat atgattgaac ttaaacaggt gagtaaatct
     3361 tttggagaac gagagttatt ttcgaatctt tcaatgacat ttgaggctgg aaaagtctat
     3421 gccttaattg gttcaagtgg tagcggaaaa acaaccttga tgaacatgat tgggaaatta
     3481 gaaccttatg atgggacgat tttttaccga ggtaaagact tggccaatta taaatcgagt
     3541 gattttttcc gtcacgaatt gggctacctc ttccagaact ttggcttaat tgaaaaccaa
     3601 agtattgaag aaaaccttaa gctaggtctc attggtcaaa agttgagtcg gtcggaacag
     3661 cggttgaggc agaagcaggc tttagaacag gtcggcctgg cttatcttga cctagataag
     3721 cgcatctttg agttatcggg cggagaatcg caacgggttg ccttggcaaa aattatctta
     3781 aagaatccac cctttattct ggcagatgag ccaacagctt caatagaccc agcaacctct
     3841 cagttgatta tggagatttt gctatctctt cgagatgata ataggctaat cattatcgca
     3901 acacataatc cggcaatttg ggagatggct gatgaagtgt tcacgatgga tcatctgaaa
     3961 taaaaatcct tgtttttaat tgcacgatga gttactgaaa tattatcatg aatcaagaat
     4021 tggagttaat ttagaattgt actttattaa tattgaggta actttttctt gataaaggaa
     4081 gaaataatgg agaggaagtt agaatgaaaa aattcgacaa ttatattatt gagaagcctt
     4141 gcgattctaa ttcagataaa ctgcaaaaaa tcttaataat tgaaagtttg gtagatgata
     4201 ttttgcaatt ttctctcaga atcaataata gtgtaggaga gattttcctc ctacaaccgt
     4261 tttaaaagaa aactatcttt atcccaaatc attcatacct ctctcaatta gatgtaactt
     4321 acaaaacccc tgacctcatg agccactttc ttcctcctca tgaggtcagt tttactttct
     4381 gctgttccag tatcgttttt cctcgctaga tttcctcaaa agggcagact cctcccttgg
     4441 tgcgtcacac gattttttca tctcgactgt tctttaatgc atcattaacg acgctttttt
     4501 tctaggtggt tcataaggaa caggaagatt caggttgact tttctaatcc tagaataaag
     4561 tgctgaaaac aattcggaat aggcatagag actagacaat ttgaggagct gcttgcgtcc
     4621 tgttcgaa
//
LOCUS       DAVLRT010000104         4496 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLRT010000104 DAVLRT010000000
VERSION     DAVLRT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.104"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            519..728
                     /locus_tag="VXM85_001917"
     CDS             519..728
                     /locus_tag="VXM85_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1346435.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            718..1206
                     /locus_tag="VXM85_001918"
     CDS             718..1206
                     /locus_tag="VXM85_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346436.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            1322..2782
                     /locus_tag="VXM85_001919"
     CDS             1322..2782
                     /locus_tag="VXM85_001919"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1346437.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISYMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            2779..3603
                     /gene="nadE"
                     /locus_tag="VXM85_001920"
     CDS             2779..3603
                     /gene="nadE"
                     /locus_tag="VXM85_001920"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1346438.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            3900..4451
                     /locus_tag="VXM85_001921"
     CDS             3900..4451
                     /locus_tag="VXM85_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1346439.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1336 a          839 c         1026 g         1295 t
ORIGIN      
        1 tcttcctttt tattctttac ctgtatttgt gacttcaaat acgatgtcta ttagtcagta
       61 tcttgtggta atcactacaa tcattttatt gtactttttc actctcaaga aaagcaagaa
      121 gtattcattt tagtttcatt tagtattatt ttgcatacct aaaatacaat aaaaaaatca
      181 gtcatcttgg tatgctcctg ctttcactat tcaacacgtt tttgacttat actaggctca
      241 tttccaaaag cattatataa tagtgatatg aaaccaacta aactaaacaa gaaatataag
      301 caataaaaac tcgtttaaaa gattctacta aagctaatac taaataaaaa taaaagagta
      361 aactaggaag tttatttcaa acaacctaaa atactgattt tcggctgaag ataatactgg
      421 agtgcaaatt aatggggtta taataaatag ctgatagctt gtgttggttt tggatttttt
      481 aagagtagat gagtattaaa actataagga ggacgaaggt ggctaaaaat ttaaaattaa
      541 aattagctcg ggtagagcgt gatttaacac aaggtcaact ggcagaggct gtcggggtga
      601 cacgccagac tattggtttg atagaggcgg gaaaatacaa tcccagtctc tcgctctgcc
      661 agtctatttg cagatgttta gggaaaaccc tagaccaact attttgggag gaagaagatg
      721 aaaaatagat tttattatta tcaattacta gacgaaagag aagaacaact gttcaataaa
      781 gcgggctctg aaagtttcta tatctgcatt gctttgtcgc tcctatctta tatcatttca
      841 gtattagcac caagcctttt taattctaat atgctgctaa tcgttatcat catagggaca
      901 ttttactttt tcaatcgtgc ccgttatctg ggagtgacct actatagtcg ttttcatttt
      961 acgattttgg gttgtttttt cctaaccttg gctattacgg ctcttttgat gttgcagaat
     1021 tatcaattca acatagaaat ttatcagcac aatcctttga attttaaata cctgtctgct
     1081 tgggtcatta cttatgtcat ttaccttccg tgggtcttta ttggcaatct tggtcttaag
     1141 agctatggcg aatgggctca gaaaaaattt gaacaagata tggatgaatt ggagagtgga
     1201 gaatagcttg ttactctttt ctcaatccag ctaaaatgtg atataatagt attaatttat
     1261 tggaatacat gaaagttctt gaaaattttc atgggtttct agctaaggaa gtaggaaaag
     1321 tatgtatcca gatgatagtt tgacattgca cacggacttg taccagatca acatgatgca
     1381 ggtttacttt gaccaaggga ttcacaataa gaaggcggtc tttgaggtgt atttccgcca
     1441 acagcctttt aagaacggct atgcggtttt tgcaggtttg gaaagaattg tgaactatct
     1501 tgaagacttg cgtttttcag atagtgatat agcctatttg gagtcgcttg gttatcatgg
     1561 ggcgttcttg gattaccttc gcaatttcaa gttggagttg accgttcgtt ctgcccaaga
     1621 aggggatttg gtttttgcta atgaaccgat tgtgcaggtg gaaggacctc tagcccaatg
     1681 tcagttggtc gaaacggctc ttttgaacat cgtcaactac cagaccttgg tggcgacgaa
     1741 ggcagctcgt attcgttcgg ttatcgaaga tgaacccttg atggagtttg ggacacgtcg
     1801 ggctcaagaa atggatgcgg ccatctgggg aacacgcgca gctgtgattg gtggcgccaa
     1861 tggaaccagc aacgtgcgtg cgggtaagct ctttgacatt cctgttttgg gaacccatgc
     1921 ccatgccttg gtacaggttt atggaaatga ctatgaagct ttcaaggctt acgctgcgac
     1981 ccacaaaaat tgtgtctttc ttgtggatac ctatgacacc cttcgcatcg gtgtaccagc
     2041 tgccattcag gtggcgcgtg agctgggtga tcagattaac tttatgggtg tgcggattga
     2101 ctctggggat attgcctaca tttctaagaa agtccgtcag caactggacg aggctggatt
     2161 tacagaggct aagatttatg cttctaatga tttggacgaa aatactatcc tcaacctcaa
     2221 gatgcaaaag gccaagattg atgtctgggg cgtgggtacc aagctgatta cagcatatga
     2281 ccagccagct cttggggcgg tttacaagat tgttgcaatc gaagatgaaa ctggtcagat
     2341 gcgcaatacg attaagctgt ctaataatgc tgaaaaagtc tctacgccag gtaagaagca
     2401 ggtgtggcgc attaccagtc gtgaaaaagg taagtcagaa ggtgactaca tcacttatga
     2461 tggtgtggat attagctaca tgacagaaat caagatgttc catccgacct atacatacat
     2521 caagaagacg gttcgtaatt ttgatgccgt tcctctcttg gtggatatct tcaaagaagg
     2581 aatattagtt tacaacttgc ctagtttgac tgacattcag gattatgccc gtaaagaatt
     2641 tgacaagttg tgggatgagt ataagcgtgt gctcaatccg cagcactatc cagtggattt
     2701 ggcgcgtgat gtatggcaag ataagatgga cttgattgat aagatgcgca aggaagccct
     2761 tggtgaagga gaagaagaat gagtttgcaa gaaacgatta tccaagagct gggtgtcaaa
     2821 ccagtgattg atgcccagga agaaatccgt cgttctattg atttcttaaa aagatatctg
     2881 aaaaaacatc ccttcctaaa aacctttgta ctagggattt ctgggggaca agactcaacc
     2941 ttggcagggc gtttggcgca attagctatg gaagaactgc gagctgaaac gggagacgac
     3001 agctacaaat ttatcgctgt ccgcctgcca tacggagtgc aagctgatga agcagatgct
     3061 caaaaagccc tagccttcat ccagccagat gtcagcttgg ttgtgaatat caaggaatca
     3121 gctgatgcca tgacagctgc agttgaagcg acaggtagtc ctgtttcaga cttcaacaag
     3181 gggaatatca aggcacgttg ccgtatgatt gctcagtatg cccttgctgg ttcccatagc
     3241 ggagcggtca ttggaacaga ccacgccgcg gaaaatatca caggtttctt taccaagttt
     3301 ggtgacggcg gtgcggatat tctccctctt taccgcctca ataaacgcca aggaaaacag
     3361 ctcttgcaga aacttggcgc agagccagcc ctttatgaaa aaatcccaac ggcagaccta
     3421 gaagaagata aaccaggcct agctgacgaa gtcgcacttg gagtcaccta tgcagagatt
     3481 gacgattacc tagaaggcaa aacaatcagc ccagaagctc aagcgaccat tgaaaactgg
     3541 tggcacaaag gccaacacaa acgccactta cccatcaccg tatttgatga cttttgggag
     3601 taaaaacctc cattggaggt ttttaccctg agcctagaaa tgagaaagtg agagaaagtc
     3661 cgggggatct tgaaccccga gtttagaaat aagaaaatga ggcagattcg gtaactcgaa
     3721 gagttcgatt tcatcgtctt acccctgcaa cgatgactag gtttgaaaaa gcttgctaga
     3781 tcgcatttca aaccaggcag cgactgcgtc aagaaattag aagacaaact cgttttctag
     3841 ctgttactga gttgagtctt tttactacga gcatagaaat aaggaagtga ggtagcatca
     3901 tgaaatctat cggtacgcaa atattacaga cagaacgttt gattttaaga agatttgtgg
     3961 agagtgatgc agaagccatg tttcaaaatt gggcttcatc cgctgagaat ttgacctatg
     4021 ttacctggga tccccatcct gatgtcgaaa tcactcgaaa ctcgattcgc aattgggttg
     4081 cttcctatac taatctcaac tattacaaat gggccatttg tctaaaagaa aacccagagc
     4141 aagtgatagg agatatcagc atcgttaaga tagacgaggc tgatttaagc tgtgaaatcg
     4201 gctatgtgct aggcaaggct tactggggac atggtatgat gacagaggcc ttgaaagctg
     4261 tcttggactt ttgttttact caagcaggtt ttcaaaaggt cagagcacgt tatgccagtc
     4321 tcaacccagc ttcaggtcgt gtcatggaaa aggctggaat gtcctatcta caaaccattg
     4381 ttaatggtgt agagagaaaa ggctatcttg cggatcttat ttattatggt ataagtaggg
     4441 aagaatgttg aattctattt tctgtttcta tcgaagtcaa ctatttattg taaata
//
LOCUS       DAVLRT010000105         4203 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLRT010000105 DAVLRT010000000
VERSION     DAVLRT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.105"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            229..2730
                     /locus_tag="VXM85_001922"
     CDS             229..2730
                     /locus_tag="VXM85_001922"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1346440.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDTSKPKFYALDMFPYPS
                     GVGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDISREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            3030..3722
                     /locus_tag="VXM85_001923"
                     /pseudo
     CDS             3030..3722
                     /locus_tag="VXM85_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            3734..4150
                     /locus_tag="VXM85_001924"
                     /pseudo
     CDS             3734..4150
                     /locus_tag="VXM85_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1263 a          797 c          969 g         1174 t
ORIGIN      
        1 agcattccag ttttatcttc ggtctaaaat aagtattttg tgctatacga gataagcgtc
       61 ttgacttact ccttgattta ctgcataaca atgggataaa aagtgggaga tagagcaatt
      121 catagtcatc aaaattaatg agatacagta tacagttttt tctttaaaca catttcaaat
      181 tccctcaaaa atggtataat agtaacatca caaaattgga gagagaccat gagtttttat
      241 aatcataaag aaattgagcc taagtggcag ggctactggg cagaacatca tacatttaag
      301 acaggaacag atacatcaaa acctaagttt tatgcgcttg atatgttccc ttatccgtct
      361 ggagttggtc tgcacgtagg acacccagaa ggttatactg caaccgatat cctcagtcgt
      421 tacaaacgtg cgcaaggcta caatgtcctt cacccaatgg gttgggatgc ttttggtttg
      481 cctgcagagc aatacgctat ggatactggt aatgacccag cagaatttac agcggaaaac
      541 attgccaact tcaaacgtca aatcaatgcg cttggatttt cttatgactg ggatcgtgaa
      601 gtcaacacaa cagatccaaa ctactacaag tggactcaat ggattttcac caagctttac
      661 gaaaaaggct tggcctatga agctgaagtg ccagtaaact gggttgagga attgggaact
      721 gccattgcca atgaagaagt gcttcctgac ggaacttctg agcgtggagg ctatccagtt
      781 gtccgcaaac caatgcgcca atggatgctc aaaatcacgg cttacgcaga gcgcttgctc
      841 aatgacttag atgaactaga ttggtcagag tctatcaagg atatgcaacg caactggatt
      901 ggtaaatcaa ctggtgccaa tgtaactttc aaagtaaaag gaacagacaa ggaatttaca
      961 gtctttacta ctcgtccgga cacacttttc ggtgcgactt tcactgtctt ggctcctgaa
     1021 catgaattag tagacgctat cacaagttca gagcaagcag aagctgtagc agactataaa
     1081 caccaagcca gccttaagtc tgacttggtt cgtacagacc ttgctaagga aaaaactggt
     1141 gtttggacag gtgcctatgc catcaaccct gtcaatggta aggaaatgcc aatctggatt
     1201 gcggactatg ttcttgctag ttatggaaca ggtgcggtta tggctgtgcc tgcccacgac
     1261 caacgtgact gggaatttgc caaacaattt gaccttccaa tcgtcgaagt acttgaaggt
     1321 ggaaatgtcg aagaagctgc ctacacagag gatggcctgc atgtcaattc agacttccta
     1381 gatggattga ataaagaaga cgctattgcc aagattgtgg cttgcttgga agaaaaaggc
     1441 tgtggtcagg agaaggttac ctaccgcctc cgcgactggc tctttagccg tcaacgttac
     1501 tggggtgagc caattccaat cattcattgg gaagatggaa cttcaacagc tgttcctgaa
     1561 actgaattgc cgcttgtctt gcctgtaacc aaggatatcc gtccttcagg tactggtgaa
     1621 agtccactag ctaacttgac agattggctt gaagtgactc gtgaagatgg tgtcaaaggt
     1681 cgtcgtgaaa ctaacaccat gccacaatgg gctggttcaa gctggtacta cctccgctat
     1741 attgacccgc acaatactga gaaattggct gatgaggacc tcctcaaaca atggttgcca
     1801 gtagatatct acgtgggtgg tgcggaacat gctgtacttc acttgcttta tgctcgtttc
     1861 tggcataaat tcctctatga cctcggtgtt gttccgacta aggaaccatt ccaaaaactc
     1921 tttaaccaag ggatgatttt gggaacaagc taccgtgacc accgtggtgc tcttgtggca
     1981 accgacaagg ttgaaaaacg tgatggttcc ttctttcatg tagaaacagg ggaagagttg
     2041 gagcaagcgc cagccaagat gtctaaatcg ctcaagaacg ttgttaaccc agacgatgtg
     2101 gtggaacaat acggtgccga tacccttcgt gtttatgaaa tgtttatggg accactcgat
     2161 gcttcgattg cttggtcaga agaaggtttg gaaggaagcc gtaagttcct tgaccgagtt
     2221 taccgtttga ttacaagtaa agaaatcctt gcggaaaaca atggtgctct tgacaaggtt
     2281 tacaacgaaa cagtcaaagc tgttactgag caaattgagt ctctcaaatt caacacagct
     2341 attgcccaac ttatggtctt tgtcaatgct gctaacaagg aagataagct ttatgttgac
     2401 tatgccaaag gctttattca attgattgca ccatttgcac ctcacttggc agaagaactc
     2461 tggcaaacag tcgcagaaac aggtgagtca atctcttatg tagcttggcc aacttgggac
     2521 gaaagcaaat tggttgaaga tgaaattgaa attgttgtcc aaatcaaagg aaaagtccgt
     2581 gccaaactca tggttgctaa agatatatca cgtgaagaat tacaagaaat cgctttagct
     2641 gatgaaaaag tcaaagcaga aattgacggt aaggaaatcg tgaaagtaat tgcggtaccg
     2701 aataaactcg ttaatatcgt cgttaaataa cgagtttatt agctctatct gccaccttca
     2761 atagtccact ggactattga agccaactaa attagttaac attgttgtga aataagatag
     2821 gaatccttca gagtagaatc tggaggattt tttgaacctt cttatgaaag tatgatatat
     2881 agtagactga atctaaaata gtacgaaaca attgctaaaa catttataga aattaatttt
     2941 actttcccaa tcgatttgtt ctcatcttat ttcaatacgc tatactatgg gcaactataa
     3001 agtttgaaaa gcgaaacaag gagaaagtta taccagtaaa cgaatatggt cagatgattg
     3061 gtgagtcaat ggaaggttat acaccaggtg aactgacttt tattgatctc ttagaagggc
     3121 gttatgctag gatagaggct ctttcagtgg aaaagcatgc ggaggatttg ctagctgttt
     3181 atggtcctga tactcctcag gagatgtgga cctacctctt tcaggagcca gtagcagaca
     3241 ttgaggaact ggtcagcctt ttaaatcaga tgttggtcgt aaggaccgtt tttactatgc
     3301 aatcatagac aaggcaactg gtaaggcttt gggaactttt tctctcatgc gtattgacca
     3361 gtaacggagt aatagaagtg ggagctgtca ctttttctcc aaaactcagg gggacacgga
     3421 tagggacaga agcccagtat ctactggctc gctatgtctt tgaagagctt aactatcgtc
     3481 gttatgagtg gaaatgcgat gctcttaacc tgccatccag acgtgctgcg gagcgtttgg
     3541 gctttattta tgaaggaacc ttccgtcagg cagtggttta taaggggcgt acgagagata
     3601 tggattggtt gtctatgatt gataaggact ggcccaaagt caaagatcgt ttggaaacat
     3661 ggttgcgtcc tgaaaatttt gataaaaatg gacagcagta caagagcttg agagagtttt
     3721 aagaggtgtt gacatgatca ctattagaaa gcaagaaatc gtcaagttcg aggatgtttt
     3781 gcatctctat caggctgtcg gttggacaaa ttatacaaat caacctcaga tgctggagta
     3841 gaccttgtat cattcattaa taatttatct ggcacttgat ggtgatgctg ttgtgggctt
     3901 gattcgtttg gttggagatg gtttttcatc agtttttgta caggatttga ttgttttgtc
     3961 tagctatcag cgccaaggga ttggtagctc cttgatgaaa caggctttag gaaattttaa
     4021 agaggcctat caagtccagc tggcgacaga acagacagaa aaaaacgtgg gatttaatcg
     4081 ttctatgggc tttgaaatct tatccaccta taattggata gggatgactt ggatgaatcg
     4141 taaaaaataa caaaacttgt tttttcttaa gcaaaattta aggatggtct agtattatat
     4201 aat
//
LOCUS       DAVLRT010000106         4186 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLRT010000106 DAVLRT010000000
VERSION     DAVLRT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.106"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            721..1713
                     /locus_tag="VXM85_001925"
     CDS             721..1713
                     /locus_tag="VXM85_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1346441.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1715..2533
                     /locus_tag="VXM85_001926"
     CDS             1715..2533
                     /locus_tag="VXM85_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1346442.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2535..3320
                     /locus_tag="VXM85_001927"
     CDS             2535..3320
                     /locus_tag="VXM85_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1346443.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3418..3636
                     /locus_tag="VXM85_001928"
     CDS             3418..3636
                     /locus_tag="VXM85_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1346444.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3621..3818
                     /locus_tag="VXM85_001929"
     CDS             3621..3818
                     /locus_tag="VXM85_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1346445.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
BASE COUNT         1161 a          720 c          907 g         1398 t
ORIGIN      
        1 cgctatatat tatggtatcg aatcttcatc agaatgataa aattaatcaa ttgatatctg
       61 attacaaaca gaatatgaaa gctttttata tcactattga aaaatttata cgagatgatg
      121 aaagccttaa gtgttatttt ataaaggtta tttcaagtcg ttccaaggta acaagtctag
      181 atcagattga agctgataaa acgatacaaa gaaaatattc aagtgagcta aaaaaattta
      241 ttggatttta taatgagatt atttgtgagg aaaatagttt cctacatgta cgaaagaggt
      301 ggtcgagttg gtttaggtag tcgatgcgtg agttgataat tctcagggta tggacttctt
      361 tttcatgaat gaggtaaaag agcaggtatt gtttagagac aatcattctg agcatatttt
      421 ctggatagag ggagtatccg attttatgat caaagttaat accgccctct ggtgagaaga
      481 tgagtaggtt ggtaatttaa actattaaac agaatttttg attaaaagta ttattttatg
      541 agagaaatcc taatttcaca atccataggc aaactcttgc atttcgtttt ttattggact
      601 ataataggtt ggtataaagc cttttgtagt aataaaatgt agaaggtgta gaaagcaagg
      661 atttagaata tttgtagtta aaaacacaat gttgctattc cttacgatag ggagatagat
      721 atggcaatga tagaagtgga acatcttcag aaaaattttg tgaagactgt taaggaaccg
      781 ggcttgaagg gggctttgcg ctcctttatt catcctgaaa agcagacctt tgaagcggtc
      841 aaggatttga cctttgaggt tccaaaaggg cagattttag gatttatcgg ggcaaatggt
      901 gctgggaagt cgacaaccat taaaatgctg acaggaattt tgaaaccaac atctggtttt
      961 tgtcggatta acggcaagat tccccaggac aatcggcaag attatgtcaa agatattggc
     1021 gtagtctttg gacaacgcac ccagctatgg tgggatttgg ctctgcaaga gacctacact
     1081 gtcttaaaag agatttatga tgtgccaaac tcgctctttc ataagcgcat ggactttttg
     1141 aatgaagtct tggatttgaa ggactttatc aaggatcccg tgcggactct ttcactggga
     1201 caacggatgc gggcggatat tgcggcctcc ttgctccaca atcccaaggt tcttttttta
     1261 gatgagccga ccattggttt ggacgtttcg gttaaggata atattcgtcg ggcaattact
     1321 cagatcaatc aagaggaaga aactaccatt cttttgacca ctcacgattt gagtgatatt
     1381 gagcaacttt gtgatcggat tttcatgatt gacaaggggc aagagatttt tgatggaacg
     1441 gtgagccaac tcaaggagac ctttggtaag atgaagactc tctcttttga actgctacca
     1501 ggtcaaagtc atctcgtctc tcactatgaa ggtctgtctg atatgaccat tgatagacaa
     1561 ggaaacagcc tcaacattga atttgatagt tctcgctacc agtcagctga cattatcaag
     1621 caaaccctgt ctgattttga aatccgcgat ttgaagatgg tggatacgga tattgaggat
     1681 attatccgtc gcttctaccg aaaggagctc taggatgatc aaattgtgga gacgttataa
     1741 accctttatc aatgcagggg ttcaggagtt gattacttac cgagtcaact ttattctcta
     1801 tcggattggc gatgtcatgg gggcttttgt ggccttttat ctctggaagg ctgtctttga
     1861 ttcttcgcaa gagtctttga ttcagggctt cagtatggcg gatatcaccc tctacatcat
     1921 catgagtttt gtgaccaatc ttctgactag atccgattcg tcctttatga ttggggagga
     1981 ggtcaaggat ggctccatta tcatgcgttt gttgcgacca gtgcattttg cggcctccta
     2041 tcttttcacc gagcttggtt ccaagtggtt gatttttatc agcgttggcc ttccattttt
     2101 aagtgtcatt gtcttgatga aaatcatatc gggtcaaggt attgtagagg tgctaggatt
     2161 aactgtcctt tatcttttta gcttaacgct cgcctatctg attaactttt tctttaatat
     2221 ttgctttgga ttttcagcct ttgtgtttaa aaatctttgg ggttccaacc tacttaagac
     2281 ttccatagtg gcttttatgt cggggagttt gattcccttg gcattctttc caaaggttgt
     2341 ttcagatatt ctttcctttt tgcctttttc atccttgatt tatactccag ttatgatcat
     2401 tgttggaaaa tacgatgcca gtcagattct tcaggcactc cttttgcagt tcttctggct
     2461 cttagtgatg gtgggattgt ctcagttgat ttggaaacgg gtccagtcct ttatcaccat
     2521 tcaaggaggt tagtatgaaa aaatatcaac gaatgcatct gatttttatc agacaataca
     2581 tcaaacaaat catggaatat aaggtggatt ttgtggttgg tgtcttggga gtctttctgg
     2641 ctcaaggctt gaatctcttg tttctcaatg tcatctttca acatattcca ttcctagaag
     2701 gctggacctt tcaagagata gctttcattt atggattttc cttgattccc aagggaatgg
     2761 accatctctt ttttgacaat ctctgggcac tagggcaacg cctagtccga aaaggggagt
     2821 ttgacaagta tttgactcgt cccatcaatc ctctctttca catcctagtt gaaacctttc
     2881 agattgatgc cttgggtgaa ctcttagtcg gtggtatttt attgggaaca acagtgacca
     2941 gcattgtttg gactcttcca aaattcctgc ttttcctagt ttgtattcct tttgcgacct
     3001 tgatttatac ttctcttaaa atcgcaacag ccagtatcgc cttttggact aagcagtcag
     3061 gcgccatgat ttacatcttc tatatgttca atgactttgc taagtatccg atttctattt
     3121 acaattctct tcttcgttgg ttgattagct ttatcgtgcc tttcgccttt acagcctact
     3181 atccagctag ctatttctta caggaaaagg atgtgttctt taacgtagga ggtttgatgt
     3241 tgatttctct ggttttcttt gttatttccc ttaaactttg ggataagggc ttagattcct
     3301 acgaaagtgc gggttcgtaa aagctaaagt aagactaaaa tcaagaaagg aacttatgat
     3361 gtttgtaatt gaagaagtca aggatgaaaa tcaaaaaagg cagttgtcgc tgaggttttg
     3421 aaggatttgc cagaatggtt tggaatccca gaaagcacac aagcctatat agaaggaacc
     3481 acgacactgc aagtttggac cgcctatcag gagagtgatt tgactggatt tgtaaggtta
     3541 tcctattcga gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag
     3601 gtagaaaaat tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg
     3661 gttatctgca ggtcaaaaca gtggcagaag cttctaataa agattatgat cgaacaaatg
     3721 acttttatcg aggtcttggc tttaaaaagt tagagatttt ttctcagcta tggaatccgc
     3781 aaaatccttg tcagattttg attaaaaagc ttgaataata ttacttgaca tctattctca
     3841 gagtgccata ctgtaagtgt aatcgccgat ttagctcagt tggtagagca aggcactcgt
     3901 aaagcctagg ttataggtag ataaacgact gaggagttga aaaaatagat aggtagaaga
     3961 taaccgttaa gccttattct tagcggttat ttatattatt taatagcgct gatatttcat
     4021 caattatgcc tgttttcgtg tttctggtag ttgttcaagt ttattgctac tatttttgat
     4081 ggtatgaatg tgcttataat gtatcccggt taacgaaagt tttggactta tactcttcga
     4141 aaatctcttc aaaccacgtc aacgtcgcct tgccgtgcgt atggtt
//
LOCUS       DAVLRT010000107         4044 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLRT010000107 DAVLRT010000000
VERSION     DAVLRT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.107"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..504
                     /gene="lytA"
                     /locus_tag="VXM85_001930"
     CDS             <1..504
                     /gene="lytA"
                     /locus_tag="VXM85_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1346446.1"
                     /translation="PYLAKWGISREQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYP
                     KDKFEKINGTWYYFDSSGYMLADRWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNE
                     EGAMKTGWVKYKDTWYYLDAKEGAMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEP
                     DGLITVK"
     gene            882..1043
                     /locus_tag="VXM85_001931"
     CDS             882..1043
                     /locus_tag="VXM85_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1346447.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            1030..1236
                     /locus_tag="VXM85_001932"
     CDS             1030..1236
                     /locus_tag="VXM85_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346448.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            1282..1635
                     /locus_tag="VXM85_001933"
     CDS             1282..1635
                     /locus_tag="VXM85_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346449.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKKCY"
     gene            1769..2194
                     /locus_tag="VXM85_001934"
     CDS             1769..2194
                     /locus_tag="VXM85_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346450.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVKEFSLEYMTTGDILVEAFKA"
     gene            2602..3592
                     /locus_tag="VXM85_001935"
                     /pseudo
     CDS             2602..3592
                     /locus_tag="VXM85_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3582..>4044
                     /locus_tag="VXM85_001936"
     CDS             3582..>4044
                     /locus_tag="VXM85_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346451.1"
                     /translation="MPANTKVIFQETFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYSLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
BASE COUNT         1414 a          543 c          834 g         1253 t
ORIGIN      
        1 ccttacttgg caaaatgggg cattagccgt gagcagttta agtatgatat tgagaacggc
       61 ttgacgattg aaacaggctg gcagaagaat gacactggct actggtacgt acattcagac
      121 ggctcttatc caaaagacaa gtttgagaaa atcaatggca cttggtacta ctttgacagt
      181 tcaggctata tgcttgcaga ccgctggagg aagcacacag acggcaactg gtactggttc
      241 gacaactcag gtgaaatggc tacaggctgg aagaaaatcg ctgataagtg gtactatttc
      301 aacgaagaag gtgccatgaa gacaggctgg gtcaagtaca aggacacttg gtactactta
      361 gacgctaaag aaggcgccat ggtatcaaac gcctttatcc agtcagcgga cggaacaggc
      421 tggtactacc tcaaaccaga cggaacactg gcagacaagc cagaattcac agtagagcca
      481 gatggcttga ttacagtaaa ataataatgg aatgtctttc aaatcagaac agcgcatatt
      541 attaggtctt gaaaaagctt aatagtatgc gttttcttgt ggagatattt ccttcaattt
      601 ttgctactat attaaacaaa aatcaaaaag caaactagaa agttatgctc aaataaaatc
      661 taaatttgac aatgtaaacc gagtcggata gctttaagta ctgttttgag gttgaagata
      721 cgatttttga taggaactca tcaaataggt ttaatatcat caattttaga tttttaagta
      781 gcatcaataa attgcttcct tgttttgtca taattttttt atttaaaaaa ttatgacaag
      841 agtgtgctat tctttttatg agaggtgtat gaatatgata aatgtatgtg ataaatgtat
      901 gtgatgttgg aaaaagaata aaagaactta gaatatcttc aaatcttact caagataaga
      961 ttgctgagta tttgtctttg aatcaaagca tgattgccaa aatggaaaaa ggtgaaagga
     1021 atatcacgaa tggatttaag taataaagct ccaaatctta gaaaaaagtt gggagctgat
     1081 ggtgaatcgc cgatagatat ttttaaattg gtacaaaaga tagaaaattt gacgctggta
     1141 ttttatggac tcggaaagaa tattagcgga gtctgttata aaggaactca gttcagtctc
     1201 attgcagtca attcagacat gtcattagga aggtaaagat tttctttagc acatgaactg
     1261 tatcatcttt attatgatga ggtgaagaag agttcagtca gtcttatctt gattggtgaa
     1321 ggagatgaaa ctgaaagaaa agcggatcag tttgcttctt attttttaat tttcccatct
     1381 tcactgtata ggatggttga ggaaatcaga gaaaatgcca atagaactca tcttgaagta
     1441 gaagatatta taaaattggg tcagttttat ggtatcagtc ataaagctat gttatataga
     1501 ttgaggaatg atggatacct tgatgcagaa gaaattaaaa atatggatat tagtgttata
     1561 gagacagctt caagattagg ctatgataca agtttatatc gtcctttgtc agaaagtaaa
     1621 aagaaatgtt attaggataa tatattaatc taactgaaca acttttagaa aataacagaa
     1681 tttcgcaagg gaagtatgag gaactgttac tagatgcttt cagatatgat attgtatatg
     1741 ggctagatga agagggggga gttgtcgttt gactagtcgt gtatttattg atgcagattg
     1801 tatttcagta tttttatggg ttggcactga acatctttta gaaaagctct atttgggtaa
     1861 aattgttatt ccacaagagg tgtatgatga aatcaatata cctacaattc cccatttaaa
     1921 atctaggata gatcagttgg tagctaaggg ttcagctgag attgtgagca tagacattgg
     1981 aactgaagaa tacgcattat atagagattt aacaagaaat catgatagta acaagattat
     2041 tggtaaggga gaagcggctt ctatttcctt agcgaaaaag cataatggga tattaggaag
     2101 taataaccta agagatgtta aatcatatgt aaaagaattt tctttagaat atatgacaac
     2161 aggagatata ctggttgaag cgtttaaagc gtaatttatt actgaacaag agggcaatca
     2221 tatctggaat aatatgctta aaaagagaag gaaaattggt gcaaattcat tttcagacta
     2281 tcttcgtgga agtattcatc aaaatagaca aaaataaatt tggataaatc gaactcacta
     2341 ttcaggaggc atatgagcaa ttcgaaaaag aaaagtgtca aattgagcct ataggagtag
     2401 aagtgaaata gtaagtcctg catagtggat gagagaaaag ttctccttga agttttcctg
     2461 aactatcagt cgcatgtcaa acgatatgta gggtaatgtg agaggggata gcgagtagtt
     2521 tttggttatt ttatcaaaaa acttatattt tattataccg aatgataaaa tataataaaa
     2581 atgatagaat aaggaaaaaa catgaatgtc aaaaagataa tgtcaatttt tcaatccttt
     2641 tatgttgatg tcagtattga ggaactgact ttaccaatca gttttgtaaa aaagtttgag
     2701 tatactcaaa tgacttttca taaggaatca tttttattga ttaaagaaaa gagaaggggg
     2761 agtttgagtt catttgttac tcaggctcgc actatgggtg ataaagccaa tatggatgtt
     2821 gttttggtgt tttcgaagtt atcagacggt gaaaaaaaag caattacttc aagctagagt
     2881 tccgtttgta gactttaagg gaaacctctt cttccctaca ttgggactag tactgaatgc
     2941 gaatgatact gaagtcccta aggaattaat acctagcgaa caattaacgt ggattgcctt
     3001 tttattgaca aaaggtcaaa aagtagtaga tgttgatttg ctttcacaag tcactggact
     3061 tccaaactca acaatttata ggtgtttgag gacttttaaa gctttatatt tgttaaacaa
     3121 gcaaaataag ctttacacat atacggtgtc aaagaaagaa ttattcttaa aatccgtgtc
     3181 atgtttattt aatcccatca aaaaacggat tttattgcca gatggcgata taaagcagat
     3241 aaaatctgtt tctaaccttc tatatggtgg tgcttatgct ttgtcgcatt caactttttt
     3301 agctgaaacg gatgaaaata ttagctatgt catatggcag agaaaattca atcagttacc
     3361 cttgccactt tctcagcata ttttaaaagg aaagatgcta gagatatgga aatatcgtcc
     3421 ttttgtatct gagttttgga atgattttaa aaataatcat gataaacaat ttgtagatcc
     3481 gatttctctt tatttgacct taaaagatga tgatgaccca cgtatagagg aagagagtga
     3541 agcactagaa aatatgatat tacagtatct gggagaagat gatgccagct aatacgaaag
     3601 ttatttttca agaaacgttt gcggattttc agaactatta tgttctgatt gggggaactg
     3661 ctacctctat cgtattggat tcgcaaggat ttaaaagtcg cacaacaaaa gattatgata
     3721 tggtcatcat tgatgaagta aaaaataagg aattttatac taccttgaat cattttttag
     3781 aattgggaga gtatcaagga agtcagaaag atgagaaagc gcagcttttt cgatttacaa
     3841 caactaatcc tgagtttcct tctatgattg aactatttag tatcttacca gaatattcat
     3901 taaagaagga cggtcgagaa attcccttac attttgacca agatgctagt ttatcagcct
     3961 tattattgga tgaagattat tataatatat tggtgcatga aaaagaaacc attcaggggt
     4021 attcggtatt gagtaattgt ggtc
//
LOCUS       DAVLRT010000108         4027 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLRT010000108 DAVLRT010000000
VERSION     DAVLRT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.108"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            10..963
                     /locus_tag="VXM85_001937"
     CDS             10..963
                     /locus_tag="VXM85_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1346452.1"
                     /translation="MKNKRIFKDFQASKMNLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1040..3028
                     /gene="uvrB"
                     /locus_tag="VXM85_001938"
     CDS             1040..3028
                     /gene="uvrB"
                     /locus_tag="VXM85_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1346453.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3038..3601
                     /locus_tag="VXM85_001939"
     CDS             3038..3601
                     /locus_tag="VXM85_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1346454.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKMNDGYYQINGNS"
     gene            3567..>4027
                     /locus_tag="VXM85_001940"
     CDS             3567..>4027
                     /locus_tag="VXM85_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1346455.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIK"
BASE COUNT         1181 a          714 c          933 g         1199 t
ORIGIN      
        1 gacggggata tgaagaataa aagaatattt aaagacttcc aagcttcaaa aatgaattta
       61 aacatttaca caagcccctt gttagccttt gtttttgtct tcataggaga gtttgtggct
      121 tatactttat atggtattag tttgttagct ctcatcggac ttgctagaaa ttttggagag
      181 gctggtcaaa atcttgcaag ctacttgcag accttgcatc agagcttgac ggataaaaca
      241 agtgactttc gtttaatttt aggattactg gcctttggtt ttattcttaa cactgtgttc
      301 agatggacaa gaaaagttga gaaaagacct attcgaacct tgggatttta tagagagaat
      361 ttcctcagca atcttctgaa aggatttagt ctaggcctgg cactttttct tctgaccttg
      421 ttaggtttag tggtcttagg tcaatatcgt ttggaatcca ttcacttgaa tccttattct
      481 cttgcctttg tcgtctttac tatcccattt tggattttac aggggacagc agaagaagtg
      541 gtggcccgtg cttggctact tcctcaattg gcctcaagaa ccaatctaaa actagctatt
      601 cttatatcta gcctgttctt taccctgctt catatgggca attctggcct cacccctcta
      661 tctctagtaa atctcttttt attcggagtt gccatggctc tttaccttct caaaactgat
      721 acagtttggg gtgttgcagg tattcatggt gcttggaatt ttgctcaggg taatctcttt
      781 gggattttag ttagtggtca accgtcagga acgtctctga tgaccttttt accacaaggc
      841 aatcaagatt ggctatcagg tggttctttt ggcatagaag gttccattat gacaagtctg
      901 gtattactac tgctgattgt ctatcttgct aataaattaa agaaagaaaa tgaaaggatg
      961 tgacttcggt ccgtcctttt cttcgtgaaa atactataag tatgctaaaa taggaatagc
     1021 acatggagag aggattctta tgatcaatca cattacagat aatcaattta aactagtatc
     1081 aaaatatcaa ccatcaggag atcaacccca agctatcgag cagttggtgg ataacattga
     1141 ggggggagaa aaagctcaga ttctgatggg ggcgactgga acagggaaga cctatactat
     1201 gagtcaggtc atttctaaag tcaataaacc aactctggtt attgcccaca ataaaactct
     1261 ggctggtcag ctctatgggg agtttaagga atttttccct gaaaatgcag ttgagtattt
     1321 cgtatcctac tatgattatt accagccaga ggcctatgtc ccttctagcg atacctatat
     1381 tgagaaggat agttctgtca atgacgagat tgacaagctt cgccactcag ctacctcagc
     1441 ccttttggag cgtaatgatg ttattgtcgt ggcctcagtc tcttgtatct atggtttggg
     1501 ttcgcccaag gaatacgctg atagtgtcgt tagtctccgt cctggtctag agatttctcg
     1561 tgataaactc ttgaatgact tggtcgatat tcagtttgaa cgcaatgata ttgatttcca
     1621 acgcggaaga tttcgcgttc gtggggatgt ggtagagatt ttcccagctt cccgagatga
     1681 acatgccttt cgagtagaat tttttggaga cgaaattgac cgtattcgtg aagttgaggc
     1741 tctgacaggt caggtgttgg gagaagtgga tcatttagcg attttcccag cgacacactt
     1801 tgtgaccaat gacgaccaca tggaagttgc cattgcaaag attcaggccg agttggaaga
     1861 acaattagct gtctttgaaa aggaaggtaa actgcttgaa gcccagcgtt tgaaacagcg
     1921 gacagagtat gatatcgaaa tgttgcgtga gatgggctat accaatgggg ttgaaaatta
     1981 ttctcgccac atggatggac ggagcgaagg agagcctcct tatacgcttc tcgacttctt
     2041 cccagatgat ttcttgatta tgattgacga gagtcatatg accatagggc aaatcaaggg
     2101 catgtacaat ggagaccgtt cgcgtaaaga aatgctggtt aattatggtt tccgtttgcc
     2161 gtctgctttg gacaatcgtc ctctacgtcg ggaggagttt gagagtcacg ttcatcagat
     2221 tgtttacgtt tcagcgacac ctggtgacta tgaaaatgaa cagaccgaga cagtgattga
     2281 gcaaatcatt cgtccaacgg gactcttgga tccagaggtg gaagtccgtc cgactatggg
     2341 acagattgat gacctcttgg gtgaaatcaa tgcccgcgtt gaaaaaaatg agcgtacctt
     2401 tatcacaact ttgaccaaga aaatggcaga ggacttgacc gactacttca aggaaatggg
     2461 tatcaaggtc aagtacatgc actcggatat caagaccttg gaacggacgg agattatccg
     2521 tgacctgcgc ttgggtgtct ttgatgtctt ggtcggaatt aacctgctcc gtgaaggaat
     2581 tgacgttcct gaagtgagcc tcgtagctat tctcgatgct gacaaggaag gtttccttcg
     2641 caacgaacgt ggactcatcc agactattgg acgtgctgct cgtaacagtg aaggccatgt
     2701 catcatgtat gcagacacgg ttacccagtc tatgcaacgt gctatcgatg aaactgcccg
     2761 ccgtcggaaa attcagatgg cctataatga agagcatggt atcgtaccac agacaatcaa
     2821 gaaagaaatc cgtgacctga ttgccgtgac caaggcagtt gctaaggaag aggacaagga
     2881 agtcgacatc aatagcctca acaaacaaga gcgcaaagaa ctcgtcaaga aactagaaaa
     2941 acaaatgcaa gaagccgtcg aagtgcttga ctttgaactg gcggctcaga tccgtgatat
     3001 gatgttggaa gtcaaggcct tggattaggg gaatagtatg atttatttaa gaaagttaaa
     3061 gagagaagat ttgatgtctt tatgggaaat ggcttattca caacttaatc cagtttggaa
     3121 acagtatgat gctccctatt atgatgatta tcagtatttt tcaaatttta aagaattcga
     3181 actacaaaaa tcagaatcca ttttaagcaa ctcaaatcgc cttggtattt ttgttgatga
     3241 taaactagtt gggactgttt cgcgttattg ggtatgtaaa caaacaagat ggatggaatt
     3301 gggaattggt atttatgata aaaaattctg gaacactggt attgggaaag ttgctatgtt
     3361 gcagtggata gataggacgt ttcaggatta cttggagttg gagcatctgg gtttgacaac
     3421 ttggtcagga aatcttggta tgatgaaact tgctgaaaaa ttaagaatga aaaaagaagc
     3481 tcatattcca aaagttcgtt attatcaagg taaatatttt gatagtatta aatatggtat
     3541 tttgagagaa gactgggaga aaatgaatga cggttattat caaatcaatg gaaactcctg
     3601 aagagataga aggtaaatcc cttgttcact ggcaaacgtg gagagaggct tatgatgacc
     3661 ttttgcctgc agaatttcag gagacaatga cattagaaag atgtcgattc tttagtcaaa
     3721 agtatccaga aaatacattg attgcgatag gtgatctgaa ggtagttggt tttatcagtt
     3781 atggtaactt tcgtgatgag actattcaag ctggtgaaat tattgcttta tatgttttaa
     3841 aagactatta cggaaaaggc atagcacaaa agttaatgaa agcagctttg actgctctga
     3901 atcatttttc tgaaattttc ttatgggtat tgaaagataa caagcgagcc attgctttct
     3961 atcaaaaaat gggttttact tttgatggac aagaaaagat acttgaactt ggaaagccta
     4021 taaagga
//
LOCUS       DAVLRT010000109         3909 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLRT010000109 DAVLRT010000000
VERSION     DAVLRT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.109"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..922)
                     /locus_tag="VXM85_001941"
     CDS             complement(<1..922)
                     /locus_tag="VXM85_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1346456.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSL"
     gene            complement(1040..1354)
                     /locus_tag="VXM85_001942"
     CDS             complement(1040..1354)
                     /locus_tag="VXM85_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEV1346457.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(1484..1723)
                     /locus_tag="VXM85_001943"
     CDS             complement(1484..1723)
                     /locus_tag="VXM85_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEV1346458.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(1775..3211)
                     /locus_tag="VXM85_001944"
     CDS             complement(1775..3211)
                     /locus_tag="VXM85_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1346459.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(3401..3739)
                     /locus_tag="VXM85_001945"
     CDS             complement(3401..3739)
                     /locus_tag="VXM85_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV1346460.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVKEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
BASE COUNT         1296 a          784 c          477 g         1352 t
ORIGIN      
        1 ataaagattt ttttaaaact tcttcagaaa tattcttctg atttttttga cattgttgac
       61 tcttagctaa caaatgcaaa gtaatgtagt ctatttcctg aactggaaat tcctgatttg
      121 ttacttctct tactttagaa agaattcttt gagcaacctt tctctctatt gcatcatcag
      181 tcatctgaca gtctatattt tttatttcaa atccggattt taaacgaatc acagacaatg
      241 ctatgtgaac tactaaattc tgtagtacaa aatcagatag ttttaggttg gcctcttggc
      301 attcatccaa aacaattcta gcaaattctt ctaatggaac agtttgagca aaaaagttaa
      361 attttacata gcaatgtatt gttttaaaaa attgattctc taggaaataa tttatgataa
      421 aacgtcgttt atcacgttcc tcgcctgaga cataaactcc tttattcgcc ctactctcaa
      481 tggacaaatt atactctgat aacatcactc gtatctttct gaaatcatga gataatgttg
      541 aacgactaac gtaaagttca tcagctaaat catcaaaaag aactggaact tgctcaaata
      601 ataatttatt taagataaat actaaacgat catcaccttt tgaaaccgca gttttcgtat
      661 agtcttcttc cagttcataa gtttgtctaa actcctggta agcgccttga ttctcaaaaa
      721 atatttgata cccttgacct tgttttgaaa tcaaccggac tccttgaata atcattgtct
      781 tctcaattaa tttcagtaca ttacggacag ttctatctga acaggataaa tattctgcca
      841 gttctttgct tgtaacaaaa cgttccttat tttttattaa aaattgaagg atatctttct
      901 ctttaatgtt taacacattc attccctcct aaaacgtatg ttttcatata ttgaagcata
      961 ttatacactt aaatcaattt atatcaaact caaaacaatt tatcttaact taaatattta
     1021 ttgacatttc atgtgttcat caaatattct caagaatcaa attagccatt ttttcaattc
     1081 ccattggaat aggaatatag gcttgaggag gtatttgtac aactggtttt cctgctttag
     1141 aaccagcctc ttcaaattgc ttaaagtaca tttttgtttg agggctgaca agatacaaat
     1201 caaaagctgc tgctgcgata gctttccctc cttcagtagc actaatagca tcaactacaa
     1261 tatctttccc ttttcctttt agaaactctg ttgttttctg tgccataagt gatgaagaca
     1321 ttcctgctgc acaaataatt aaagcttttg ccataatatt ttctcctttt cttaaatcca
     1381 atcaaagctg tgctaagttg gcttatttgt tatctatttt tattataaaa taaagcgttt
     1441 ccaatgacaa ttccctcatt ttcctaaatg atatggaaaa aaattattta tacttcaatt
     1501 tataaaataa aattattcct gagagtagaa atgaaacact atttgctaaa atcaaaggca
     1561 agtctcctat acgaatacca tgagcaagcc acaatgcaat accaataact tgcataacat
     1621 acatacctag agcaatagat cctgtgtcct ttgtcttaac tacacgaaaa acttgtggta
     1681 aaaatgcaaa tgttgttaaa attgctgcaa tacttccaat catatgtcac ctcaatatgc
     1741 taaacaaact gagaataatc tcagtttgtt tatactattc tactgattca ccgttagatg
     1801 aaataacttc cttataccag ccaaaagatt ttttcgggga acgattataa cttcccttcc
     1861 cattatcatc tttatctaca taaataaaac cataacgttt ccgcatttca ccggtaccag
     1921 ctgaaaccaa atcaatacat ccccatggag tataacccat taaatcaaca ccatcttcaa
     1981 ctacagcctt tttcatttca cgaatatggg cacctagata ttcaattcta taatcatcat
     2041 gtaccatacc atctgctgca acttgatcta tagctccaaa accattttca acaataaaga
     2101 gtggtaagtg atagtggtct gtaaaccaat ttaacgcata acgtaaacct tctggatcaa
     2161 tttgccactc ccattcagaa gccttaacat aattattttt cactaaatct tctgtttcaa
     2221 gataatcaaa ataaggatta ttttcacgat gagagtctat agcaaaggac atatagtaac
     2281 tgaaaccaat gtaatctaca gtcccaccaa gtaaatcttc tttatcctgg tcagtaaaat
     2341 caactgaaat accttttcgt tcccaatact tgaaaatatg ctcaggatat ttacctaaaa
     2401 catgcacatc agcaaaataa taacgcttct gcatagcttt cattgccatt aagatatcct
     2461 taggattgca agtaactgga taaattggac acatcgcaat catacaacct atttgaaaat
     2521 ctggattaat ctcatgacca atttttacag ctcgtgcaga agcaactaat tcgtaatgtg
     2581 ctgcttgata cataattgct tctctattat caccttcctc atatacaata cctgagttag
     2641 taaatggtgc aaaatcttcc tgataattcg cttgattatt gatttcattg aaagtcatcc
     2701 aatatttaac cttatctttg taacgtttaa atacgacttc tgcaaaacga gcaaagaaat
     2761 caatcaattt cctatttttc caaccaccat attcggtcac taagtgataa ggcatttcaa
     2821 aatgagatag agtgatgaca ggttcaatac cattctttaa gcattcatca aaaagattat
     2881 cataaaactg taatccttct tcattcggct ctaactcatc accttttgga aagatacgtg
     2941 tccatgcaat agaggtacgg aagcacttga atcccatttc agcaaaaagt gctatgtctt
     3001 ctttataacg atgataaaaa tctatcgcct catgatttgg ataatattta ccctctaaaa
     3061 ctcccaaagt aatttcacga gctactccat gacgaccagc agtcataaca tcagcaacac
     3121 taattccctt gccaccttct tgccatccac cttcaagttg atgagcagca acagcaccac
     3181 cccataaaaa tccatcttta aaagtagtca tcttttttcc tcctgacttt gatactctta
     3241 ttataaacct taaaccaaaa gatgaaaacg cattcttttt ccttattgtt aaggaaagaa
     3301 gtaattttta atggaaatag aacaatatct tcttgtattc tcgtaatgat atctttacga
     3361 ttttcaatac tttcaaacta caaaaactct cacaataatt ctaattccct gtgtctataa
     3421 acgacttatc gctttctggc atcccagaat catcttctat ataacgttca acttgcatct
     3481 gcaagtgata tttttttctt aaatctaaga ttttctgcat tgtctttgat tgataatgtt
     3541 tatctaaagt ttcttgattt atccactgat caataaggag aatagttccc tctttttcaa
     3601 ttggtaaaaa atattcgtat ttcaagttac ctttttgatt tctaatttct ttaacaaggc
     3661 cactatcaag catttctctt gcaaacttta ttgcactatc tccatcacct ttataatata
     3721 catgaatagt caatgtcatc ttatatcctc caaaatcatc cttcaatttt aaaaaaacaa
     3781 gtttagatga ggatctaaac ttgtttttta tgaactaatt atctaacgtt tcgccattac
     3841 tttcaatcac ttctttatac caataaaatg attttttctt atagcgattt atagtcaatt
     3901 gaaacaaga
//
LOCUS       DAVLRT010000110         3893 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLRT010000110 DAVLRT010000000
VERSION     DAVLRT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.110"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(112..1782)
                     /locus_tag="VXM85_001946"
     CDS             complement(112..1782)
                     /locus_tag="VXM85_001946"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1346461.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2012..2701
                     /gene="coaB"
                     /locus_tag="VXM85_001947"
     CDS             2012..2701
                     /gene="coaB"
                     /locus_tag="VXM85_001947"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1346462.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2713..3264
                     /gene="coaC"
                     /locus_tag="VXM85_001948"
     CDS             2713..3264
                     /gene="coaC"
                     /locus_tag="VXM85_001948"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1346463.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETLDEKTL"
     gene            3248..3811
                     /locus_tag="VXM85_001949"
     CDS             3248..3811
                     /locus_tag="VXM85_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1346464.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT         1163 a          872 c          701 g         1157 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaatc ttattgatga actgcttgca gtctgagaaa
       61 aaatgagctt ggatattatt tccaaactca cttaaagtca atttcaatcc actagaacaa
      121 gcctagtaca gttccatcgc tttcaacatc catgttgaga gctgctggac gttttggaag
      181 acctggcatg gtcataacat caccagttaa ggcaacgatg aagcctgcac ctaattttgg
      241 taccaattca cgaatggtaa tttcaaagtt ttctggtgct ccaagcgcat ttggattgtc
      301 tgagaaactg tattgagttt tagccataca gattggcaat ttgtcccaac cgttttgaac
      361 gatttgagca atttgtgttt gagctttctt ctcaaagttc actttgctac cacgatagat
      421 ttcagtgaca attttttcaa tcttttcttg gacagaaagg tcattatcgt acaaacgttt
      481 atagttagct ggattttcag caattgtctt aacaactgtt tcggcaagtg ctactccacc
      541 ttctgctcca tcagcccaga cactagccaa ttcaactggt acatcgattg aggcacagag
      601 ttcttttaag gctgcaattt cagcttctgt atcagataca aattcgttaa tagctacaac
      661 tgctggaata ccgaacttac ggatattttc aacgtggcgt ttcaagttag caaaacctgc
      721 acgaactgcc tctacatttt cttcagtcag agcgtcttta gccacaccac cattcatctt
      781 aagggcacga agggttgcga caataacaac tgcatctgga gatgttggca agtttggtgt
      841 cttgatatca aggaatttct cagcaccaag gtccgcacca aaaccagctt cagtaacagt
      901 gtaatcagcc aagtgaaggg ctgttgtcgt cgccaaaaca gagttacagc catgagcgat
      961 attggcaaat ggaccaccgt gtacaaaggc aggtgtaccg taaattgtct gaaccaagtt
     1021 tggcttaata gcatccttca aaatcaaagc caaggcaccc tcaacctgca aatcacctac
     1081 agaaacaggc gtacggtcat agcgataacc aataacgata ttcgccaaac gacgtttcaa
     1141 gtcctcgatg tccgttgcca agcaaagaat tgccatgatt tctgaagcaa ctgtaatatc
     1201 aaaaccatcc tcacgtggaa taccgtttag aggaccacca agaccaacag tcacatggcg
     1261 gagcgcacgg tcgttcaagt ccacaacgcg tttccagagg atacgacgtt gatcaatttc
     1321 cagctcattc ccttggtgca agtggttgtc aatcaaggca gaaagggcat tgttggcagt
     1381 tgtaatagca tgcatatctc cagtaaagtg gaggttgatg tcttccattg gcagaacttg
     1441 tgcataccca ccaccagcag caccaccctt gatccccatg actggaccaa gagacggttc
     1501 gcggatagca atcatggttt tcttgccaat cttgttcaag gcatccgcaa gaccaatggt
     1561 aagtgtcgac tttccttcac ctgcaggtgt tgggttgatg gcagtaacca agatcaattt
     1621 accgactgga ttgctctcaa ctgcacgaat tttatcaaag ctgagtttag ccttgtactt
     1681 tccgtacaac tccaaatcgt cataagaaat accaagtttc tctacaacat caacaattgg
     1741 cttcaactca atactctgtg cgatttcaat atctgttttc attcaaaatt cctctaacct
     1801 cttatatgat aattcattat atcacaaaac aagattttta acatcctaaa actctctaaa
     1861 cgttcgtaaa tatctctgtt tttaaggctt ttagagtcct ttcttaaatt ttatatggtt
     1921 ttatagtttg aaactatagt aaatcttcgt ttttaccaaa aatttatcac tttcatttta
     1981 cttaccgctt atttttgtgt acaatagtgc tatgaaaatt ttagttacat cgggcggtac
     2041 cagtgaagct atcgatagcg tccgctctat cactaaccat tctacaggtc acttggggaa
     2101 aattatcaca gagactttgc tttttgcagg gcatgaagtt tgtttaatta cgacaaaacg
     2161 agctctgaag ccagagcctc atcctaacct aagtattcga gaaattacca ataccaagga
     2221 ccttctaata gaaatgcaag aacgtgttca ggattatcag gtcttgatcc actcaatggc
     2281 tgtttctgac tacactcctg tttatatgac agggcttgag gaagttcagg ctagctccaa
     2341 tctaaaagaa tttttaagca agcagaatca tcaggccaag atttcttcaa ctgatgaggt
     2401 tcaggttttg ttccttaaaa agacacccaa aatcatatcc ctagtcaagg aatggaatcc
     2461 tactattcat ctgattggtt tcaaactgct ggttgatgtt accgaagatc atctggttga
     2521 cattgcacga aaaagtctta tcaagaatca agcagattta atcatcgcga atgacctgac
     2581 tcaaatttca gcaggtcagc accgagctat atttgttgag aaaaatcagc ttcaaacagt
     2641 ccagactaaa gaagaaattg cagaactcct ccttgaaaaa attcaagcct atcattctta
     2701 gaaaggaaaa ctatggcaaa cattctcttg gctgtaacgg gttcaatcgc ctcttataag
     2761 tcggcagatt tagtcagttc tctaaaaaaa caaggccatc aagtcactgt cttaatgact
     2821 caggctgcta cagagtttat ccaacctttg acactacagg tactctcaca gaatcctgtc
     2881 cacttggatg tcatgaagga accctatcct gatcaggtca atcatatcga acttggaaaa
     2941 aaagcagatt tatttatcgt ggtacctgca actgctaaca ctattgcaaa actagctcac
     3001 ggatttgcgg acaacatggt aaccagtaca gctctagccc tgccaagtca tattcccaaa
     3061 ctaatagctc ctgctatgaa tacaaaaatg tatgaccatc cagtaactca gaataatctg
     3121 aaaacattag aaacctacgg ctatcagctg attgctccta aggaatccct actagcttgt
     3181 ggagaccacg gacgaggagc tttagctgac ctcacaatta ttttagaaag aataaaggaa
     3241 actctcgatg aaaaaacgct ctaatattgc acccattgct atcttttttg ctaccatgct
     3301 cgtgatacac tttctgagct cacttatctt taaccttttt ccatttccaa tcaaaccgac
     3361 cattgttcat attcctgtca ttattgccag cattatttat ggtccacgag ttggggttac
     3421 acttggattt ttgatggggc tacttagctt gacggttaac acgattacga ttctaccgac
     3481 aagctacctc ttctctccct tcgtaccaaa cggaaacatc tactcagcta tcattgccat
     3541 cgtcccacgt attttgattg gtttaactcc ttacttagtc tataaactga tgaaaaacaa
     3601 gactggtctg attttagctg gagcccttgg ttccttgaca aatactatct ttgtccttgg
     3661 aggaatcttc ttcctatttg gaaatgttta taatggaaat atccaacttc ttctggcaac
     3721 cgttatctca acaaattcaa ttgctgaatt ggtcatttct gcaattctaa ccctagccat
     3781 tgttccacga ctacaaacct tgaaaaaata aaaacaatct ccgctttcaa acctgaaggt
     3841 ggagattttg ctctatacag tttcttttta atgaaatctt tactatagta aaa
//
LOCUS       DAVLRT010000111         3882 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLRT010000111 DAVLRT010000000
VERSION     DAVLRT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.111"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..769)
                     /locus_tag="VXM85_001950"
     CDS             complement(<1..769)
                     /locus_tag="VXM85_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1346465.1"
                     /translation="MNFCFVILHYRTSNDTIECIKSIQKLEGDYKIVIVDNASQNGSI
                     ERVEALFANNDEIVIIKNTKNLGFAAGNNIGYAYARTQIKADMIAVLNNDIVIKQKDF
                     INRIFELYRNSQFHIAGPDIVSLVDGHHQSPVVEKINSISKANKELFKYRILRIINKI
                     GLYELMTRQPKKELNRISVADPMRYQENVILHGSFVIFSPLYVSEEECAFRPDTFMYM
                     EEPILYEYCMLKNYKTVFDPSIVIYHKEDSSTNSLYNA"
     gene            complement(778..1959)
                     /locus_tag="VXM85_001951"
     CDS             complement(778..1959)
                     /locus_tag="VXM85_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1346466.1"
                     /translation="MTLRIKYIHEIFLEEIDKNMKKVLIVRSGPYQVNPEGYNLQELG
                     LASAISQQGYQCDVMYYHKTKNYDQLYEKNGEQIKIFWRRGIRLFRSGIYPQILREKF
                     LNQYDKIIISEYSQIMAVLLSRIHSNVYIYNGPYYNLFKIPIIEPIYDFLFVEMLNKR
                     TKTVFCKTEKAECYLKNKGFNDCKVVGVGLDIEKFEQEEEPTEDTIELLRRMENKQNI
                     LYVGSLSKRKNTAQLIRIFNILKSKSGKKNELQLVLIGKDEDNIVEKINYSRFKDDII
                     YQPYLKNSQLQFIYPSSQLLVLPSVQEIFGMVLLEAMYFKLPVVSSASAGGETLIQDG
                     INGKIVNDFNDEHWVDCIENLLNNPLELKRLGECAHKRITEQFMWSSIARKIIETFDE
                     R"
     gene            complement(1910..3145)
                     /locus_tag="VXM85_001952"
     CDS             complement(1910..3145)
                     /locus_tag="VXM85_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV1346467.1"
                     /translation="MKIAVVGTGYVGLSISVLLAQHHHVVAVDIVKEKVELINKRISP
                     IQDDFIERYLSESNLDLVATIDAETAYKDASFIVVATPTNYDLERNFFDTSTVESVIQ
                     QVQNMNSTATIVIKSTVPVGFTDSIRKKLGISNLLFSPEFLRESRALYDNLYPSRIIV
                     GTDLSNQKLVEKAQVFSNLLVQGAIKKDIPILTMGFTEAEAVKLFANTYLALRVSYFN
                     ELDTYAEIKGLDTQKIIEGVSLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYKNVPE
                     ELIGAIVRSNTTRKDFIANQVLKVVNYNIPEDDIPTKQMEKEVVIGIYRLTMKSNSDN
                     FRQSSIQGVMKRIKDRGVKVIIFEPTLKDSDTFFGNAVINDLEEFKVKCDVIIANRYD
                     ETLNDVKDKVYTRDIFRRD"
     gene            complement(3189..3878)
                     /locus_tag="VXM85_001953"
     CDS             complement(3189..3878)
                     /locus_tag="VXM85_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV1346468.1"
                     /translation="MIIKRLIKTITGKEIDLHPDIPITYILQRGFQYGLGLSRGLLRG
                     IGLGKKGKRLFIGRGVTLLAKRKLYVGNNIRIGKYVSIDCLSKDGIQIADNVKIGDYS
                     QIIGTGSVKNMGIGLKIGKNSSFSEYSLFGSAGGITIGGDVIAGQNVRFHAENHSYEV
                     LHIPIREQGVNRKGISVGDNCWIGAGAVFLDGSSIGSGCIVATNAVVTKKFPDNVIIG
                     GVPAKILMERK"
BASE COUNT         1233 a          764 c          505 g         1380 t
ORIGIN      
        1 ctgcattata caacgaattt gttgatgagt cctctttatg ataaattaca atagaaggat
       61 caaatactgt cttataattt ttaagcatgc aatattcata tagaataggc tcttccatat
      121 acatgaatgt atccggtctg aaagcacact cctcctctga aacatacaac ggtgaaaaaa
      181 ttacaaaact cccatgtaaa ataacatttt cctgatatct catagggtct gcaacactta
      241 ttctatttaa ttcttttttt ggctgtctag tcatcagctc atataatcct attttattaa
      301 taatcctcaa gattctatat ttaaaaagtt ctttattagc cttagatata gaattaatct
      361 tctcaacaac aggactttga tgatgaccat ctacaagaga gacaatgtct ggaccagcga
      421 tatgaaactg agaatttcta tagagttcaa atatacgatt gatgaaatct ttttgcttaa
      481 taacaatatc attattaagt acagcaatca tatcagcttt tatttgagtc ctagcgtagg
      541 cgtatccaat attatttcca gcagcaaatc ctaagttttt cgtgtttttt attataacta
      601 tctcgtcatt atttgcaaat aaggcttcta ccctttctat actgccattt tgagaagcat
      661 tatcaacaat tacaatttta taatctccct caagcttttg aatcgatttg atacactcta
      721 tagtgtcatt gctggtacga taatgcaaaa tcacaaaaca aaaattcata gcaagtctta
      781 cctttcatca aatgtctcaa ttatttttct cgcaattgaa gaccacataa actgttccgt
      841 aatgcgctta tgagcacatt ctccaagccg tttcaattcc aatgggttat ttaatagatt
      901 ttctatacaa tccacccagt gttcatcatt aaaatcattc acaattttac cgtttatacc
      961 atcttgaatc agtgtctctc ctccagcact tgcactggaa acaacaggca acttaaaata
     1021 cattgcctca agtaatacca taccaaaaat ctcttgaact gacgggagca caagtagttg
     1081 tgatgatggg taaataaatt gaagctgaga attctttaaa taaggttgat aaataatatc
     1141 atctttaaac cgtgaatagt taattttttc aacaatatta tcctcatcct taccaatcaa
     1201 taccaattga agttcatttt tcttaccact tttagatttt aaaatattaa aaattctaat
     1261 taactgagct gtgttttttc tttttgatag tgaacctaca taaagaatat tttgtttatt
     1321 ctccattctt cttaaaagct ctatagtatc ctctgtaggt tcctcttctt gttcaaactt
     1381 ctcaatatct agacctacgc ccactacttt acaatcattg aatcctttgt ttttaagata
     1441 acactctgct ttttctgtct tgcagaaaac tgttttggtt ctcttattga gcatttctac
     1501 aaaaagaaaa tcgtatatag gttcaataat gggaatctta aatagattat agtatgggcc
     1561 attataaata tatacattgg aatgaattct agacaataaa actgccataa tctggctgta
     1621 ctcagaaata attatcttat catactgatt caaaaatttc tctcttaaaa tctggggata
     1681 aattccactt ctaaataatc ttattcccct cctccagaat attttaattt gttccccatt
     1741 tttctcatat aattgatcat agttctttgt tttgtgataa tacatgacat cacactggta
     1801 gccttgctga ctgatagcgg aggctaaacc aagttcttga agattatatc cttccggatt
     1861 gacttggtaa ggtccacttc gaacaattaa tacttttttc atatttttat caatctcttc
     1921 taaaaatatc tcgtgtatat actttatcct taacgtcatt caacgtttca tcataacgat
     1981 ttgcaataat cacatcacac tttactttga actcttctaa atcatttata acagcgtttc
     2041 caaagaatgt atcactatct tttagagtag gctcaaaaat gattacttta actcctctat
     2101 ccttaatacg tttcataact ccttgtattg aactctgacg aaagttatca ctgttacttt
     2161 tcatagttaa tcggtatatt ccaataacaa cttctttctc catctgttta gtaggaatat
     2221 cgtcttctgg tatattataa ttcaccactt taagaacttg attagcaata aaatctttac
     2281 gtgttgtatt gcttcgtact atagctccaa taagctcttc tgggacattc ttataattgg
     2341 ctaaaagttg cttagtatcc tttggtaagc aatatccacc atacccaaag ctcggattat
     2401 tataatgggt tccgatacga ggatctaaac taacaccttc aattattttt tgagtatcta
     2461 accctttgat ctctgcataa gtatctaatt cattaaaata tgatacccgc aatgctaagt
     2521 aggtattagc aaataattta actgcttcag cttcagtaaa tcccatagtt aaaataggga
     2581 tatctttttt aatggcacct tgcactaaaa gattagagaa aacttgggct ttttcaacta
     2641 atttttgatt acttaaatct gttccaacta tgatccgact tggatataag ttatcataaa
     2701 gtgcccttga ttcacgcaaa aattcaggac tgaatagaag atttgaaatt cctaattttt
     2761 tacgaattga atcggtaaag cccacgggga ctgttgattt tattactata gttgctgtag
     2821 aattcatatt ctgaacttgt tgaatgaccg attcaactgt tgatgtatca aaaaaatttc
     2881 tttctaagtc ataatttgtt ggtgtcgcta ctacgataaa agaggcatct ttataagctg
     2941 tttcagcgtc tattgttgca actaaatcta aattactttc tgaaagatat ctttcaataa
     3001 aatcatcctg aataggtgaa attcttttat taattaattc tactttctct tttacaatat
     3061 ctactgctac tacatgatgg tgttgtgcaa gtaacacaga aatggataaa ccaacataac
     3121 ctgtcccaac tacagcaatc ttcattttag acatatcatt tccctccatt tatacaatta
     3181 atttaatact actttctttc cattaaaatt ttagctggca ctccaccaat aattacatta
     3241 tctggaaatt tcttagtaac aaccgcattc gttgcaacga tacaacctga accgatacta
     3301 gatccatcta aaaaaacagc tcctgcccca atccagcaat tgtcaccaac ggatatccct
     3361 ttacgattaa caccttgctc tctgattggt atatgcaaca cttcgtagct atgattctca
     3421 gcatgaaaac gtacattttg tccagcaata acatctcctc cgatagtaat cccacctgca
     3481 gaaccgaata aactatattc tgagaaagaa gagttctttc caattttgag cccaataccc
     3541 atatttttta ctgaacctgt accaattatt tgcgagtaat ctcctatctt aacattatcc
     3601 gcaatttgga tcccatcctt tgaaaggcaa tctatgctca catatttgcc gattcggata
     3661 ttgtttccaa catataactt gcgttttgcg agaagggtta ccccacgtcc aataaataat
     3721 cgtttgcctt tttttcctag accaatacca cgtaacaacc ctcgactaag acccaaaccg
     3781 tattgaaaac ctctctgaag aatgtaagtt attgggatat cagggtgaag atcaatttct
     3841 ttacctgtaa ttgtcttaat taatcttttg attatcattt gt
//
LOCUS       DAVLRT010000112         3865 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLRT010000112 DAVLRT010000000
VERSION     DAVLRT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.112"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            239..2563
                     /gene="pflB"
                     /locus_tag="VXM85_001954"
     CDS             239..2563
                     /gene="pflB"
                     /locus_tag="VXM85_001954"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1346469.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(2931..3149)
                     /locus_tag="VXM85_001955"
                     /pseudo
     CDS             complement(2931..3149)
                     /locus_tag="VXM85_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3245..3493)
                     /locus_tag="VXM85_001956"
     CDS             complement(3245..3493)
                     /locus_tag="VXM85_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346470.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            complement(3486..3770)
                     /locus_tag="VXM85_001957"
     CDS             complement(3486..3770)
                     /locus_tag="VXM85_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346471.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
BASE COUNT         1224 a          748 c          708 g         1185 t
ORIGIN      
        1 ttcgatttcc attgagtgtt actgcttatt ttcttttatt ataccctttt ttctgaaaaa
       61 aagaaaaaag gactttattt tttcaaaaat ataatacagt ttgaaataaa atatagactg
      121 ttttagaaaa gaaagtgtaa aaatagggaa ttttcacttg ttgaaatcgg ttactatatg
      181 gtatacttgt cttatgaatg taacagatga ctgttactag aaaaaagagg acattaatat
      241 ggttgttaag acagttgttg aagcacaaga tatttttgac aaagcttggg aaggcttcaa
      301 aggcgtagat tggaaagaaa aagcaagtgt atcacgattt gtacaagcta actacacacc
      361 ttatgatgga gacgaaagct tccttgcagg accaacagag cgttcacttc acatcaagaa
      421 aattgtagaa gaaactaaag cacactacga agaaactcgt ttcccaatgg acactcgtcc
      481 aacatctatc gctgatatcc ctgctggatt tatcgacaaa gaaaatgaag ttatctttgg
      541 tatccaaaat gatgaactct tcaaattgaa cttcatgcca aaaggtggta tccgtatggc
      601 tgaaactact ttgaaagaaa atggatacga accagaccca gctgttcacg aaatcttcac
      661 taaatatgta acaacagtta acgacggtat tttccgtgcc tacacttcaa atattcgtcg
      721 cgctcgtcat gcacacactg taactggtct tccagatgca tactcacgcg gacgtatcat
      781 cggtgtttac gcacgtcttg ctctttacgg tgcagactac ttgatgcaag aaaaagtaaa
      841 cgactggaat gcaatcaaag aaatcgatga agaaacaatc cgtcttcgtg aagaagtaaa
      901 ccttcaatac caagcattgc aacaagttgt tcgcctgggt gacctttacg gggttgatgt
      961 tcgcaaacca gcgatgaacg tgaaagaagc aatccaatgg gttaacattg ctttcatggc
     1021 tgtctgccgt gtgattaacg gtgctgctac atctctaggt cgtgtaccaa tcgtattgga
     1081 catctttgca gaacgtgacc ttgctcgtgg tacatttact gaatcagaaa tccaagaatt
     1141 cgttgatgat ttcgttatga aacttcgtac agttaaattt gctcgtacaa aagcttatga
     1201 ccaattgtac tcaggtgacc caacctttat cacaacttct atggctggta tgggtaacga
     1261 cggtcgtcac cgtgttacta agatggacta ccgtttcttg aacactcttg acaacatcgg
     1321 taactcacca gaaccaaact tgacagttct ttggactgac aaattgccat acaacttccg
     1381 tcgctactgt atgcacatga gccacaaaca ctcttctatc caatacgaag gtgtaacaac
     1441 aatggctaaa gacggatatg gtgaaatgag ctgtatctca tgctgtgtgt ctccacttga
     1501 tccagaaaat gaagaacaac gccacaacat ccagtacttc ggtgctcgtg taaacgttct
     1561 taaagccctt cttactggtt tgaatggtgg ttacgacgat gttcacaaag actacaaagt
     1621 atttgatatc gaaccaatcc gtgacgaagt tcttgaattc gaatcagtta aagcgaactt
     1681 tgaaaaatct cttgactggt tgactgacac ttacgtagat gccttgaaca tcatccacta
     1741 catgactgat aggtacaact acgaagctgt tcaaatggcc ttcttgccaa ctaaacaacg
     1801 tgccaacatg ggattcggta tctgtggatt tgctaacact gttgatacat tgtcagctat
     1861 caaatacgct acagttaaac caatccgtga cgaagatggc tacatctacg attacgaaac
     1921 aatcggtgac tacccacgct ggggtgaaga tgacccacgt tcaaacgaat tggcagaatg
     1981 gttgatcgaa gcttacacaa ctcgtctacg tagccacaaa ctatacaaag acgcagaagc
     2041 tacagtatca cttttgacaa tcacatctaa cgttgcttac tctaaacaaa ctggtaactc
     2101 accagttcac aaaggtgtat acctcaacga agatggttct gtgaacttgt ctaaacttga
     2161 attcttctca ccaggtgcta acccatctaa caaagctaaa ggtggttggt tgcaaaactt
     2221 gaactcactt tctagccttg actttagtta tgcagctgac ggtatctcat tgactacaca
     2281 agtatcacct cgcgctcttg gtaagactcg tgatgaacaa gttgataact tggtaacaat
     2341 ccttgatggt tacttcgaaa acggtggaca acacgttaac ttgaacgtta tggacttgaa
     2401 cgatgtttac gaaaagatca tgtcaggcga agacgttatc gtacgtatct ctggatactg
     2461 tgtaaacact aaatacctca ctccagaaca aaaaactgaa ttgacacaac gtgtcttcca
     2521 cgaagttctt tcaatggatg acgccttgga tgcattgagc taatcaagtt cttgaataat
     2581 aaaaaggaac cctcggtcaa tcgactgagg gttttgtgct tgggatagtg tgaacaattc
     2641 ctttgacgca atatgcaatg tttttgggaa ggaatagtgg accgaatctg aaatataata
     2701 gattgaaact agaatagtac acctttattt ctaaaacatt cttagaaatc gatttgactg
     2761 tcctgatcga tttgtcctat tcttatttca ttttactata gtccactgaa acgtctcaaa
     2821 cagcgctaga aattgactgg aattctctaa gcgatttgtt cagattttat ttcatttcat
     2881 cataaaaaac aagacgactg gctaattgac aatcatcttg tttgatactg ttaataaaat
     2941 tcgcgaagtt tatcttgatt ttctttaaaa aatttttcta accaatccat accataagca
     3001 taactaaaac cttctaatag aacagagagg tcaatagtat caaatcccat gtcttttaaa
     3061 tatttaaagg tatcgaatgg tgaatgaaag tttttgtatt taataattgc accaatatct
     3121 tgcaaatcct actctctgat actcatcatc ttcattccta acacatactc aatagggact
     3181 actaaaacgg tcagattctc aaatgagtac aaggattcgc ataaacttaa gggaggttgt
     3241 ttgttcatat ttgctttgca acatgattgt tgagccataa ctcttcgtgg atgttaagat
     3301 taaactgttt tcctacacga gcaataattt cattgatttt ctgattttgg tcataaaaag
     3361 catcaacatc ttgtgtggca cgtaaaccat gatattctaa gacataacca tcgacacaga
     3421 taattgttaa agtcagattt tcctgaatta attccttatt tagggcttgg aagaccggtt
     3481 tcattttatc catgtttgta tttctccatc aatttttgaa cattggttaa cttagcaggt
     3541 ttatttaatg aaatcacata tttatgagtt ggagtatgct cttgaaaggc atacttcaag
     3601 ctaatttttt gaaaacgggt tagttctttg gagtctagca acttttctac atcttggttt
     3661 gttaaataag aatagtcttc aaaaataact tttcgcaaat agcgaaacca atggcttgcg
     3721 ttgaattgtg attgtttgga tgcctgtatc atataatcgt accaatccat tgtcttcctc
     3781 ctataaaatt tagtttatat tattgtagtt tatattcgaa aatgtagttt ttcttaatta
     3841 gctcgtatct ataggatgat actga
//
LOCUS       DAVLRT010000113         3809 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLRT010000113 DAVLRT010000000
VERSION     DAVLRT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.113"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..429
                     /locus_tag="VXM85_001958"
     CDS             <1..429
                     /locus_tag="VXM85_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1346472.1"
                     /translation="DHVRKNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSA
                     DNLYKPSTDTEETEEEAEDTTDETEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAM
                     ETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            639..3758
                     /gene="phtE"
                     /locus_tag="VXM85_001959"
     CDS             639..3758
                     /gene="phtE"
                     /locus_tag="VXM85_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1346473.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNLADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1424 a          623 c          750 g         1012 t
ORIGIN      
        1 gaccatgttc gtaaaaataa ggcagaccaa gacagtaaac ctgatgaaga taagggacat
       61 gatgaagtaa gtgagccaac tcaccctgaa tctgatgaaa aagagaatca cgctggttta
      121 aatccttcag cagataatct ttataaacca agcactgata cggaagagac agaggaagaa
      181 gctgaagata ctacagatga gactgaaatt cctcaagtag agcattctgt gattaacgct
      241 aagatagcag aggcggaggc cttgctagaa aaagtaacag attctagtat tagacaaaat
      301 gctatggaga cattgactgg tctaaaaagt agtcttcttc tcggaacgaa agataataac
      361 actatttcag cagaagtaga tagtctcttg gctttgttaa aaaaaagtca accggctccg
      421 atacagtagt aaaatgaatg gagcatattt tatggagaag taacctttcg tgttacttct
      481 cttttttaga aaaacgtaac agaaacttga caaaagtaat tttaaatagt aaactattta
      541 ctggttaatt aaatggttaa ataaccggtt tagaaaacta tttaataaag taaaagaagt
      601 tgagaaaaaa cttcatcatt tattgaaatg agggatttat gaaatttagt aaaaaatata
      661 tagcagctgg atcagctgtt atcgtatcct tgagtctatg tgcctatgca ctaaaccagc
      721 atcgttcgca ggaaaataag gacaataatc gtgtctctta tgtggatggc agccagtcaa
      781 gtcagaaaag tgaaaacttg acaccagacc aggttagcca gaaagaagga attcaggctg
      841 agcaaattgt aatcaaaatt acagatcagg gctatgtaac gtcacacggt gaccactatc
      901 attactataa tgggaaagtt ccttatgatg ccctctttag tgaagaactc ttgatgaagg
      961 atccaaacta tcaacttaaa gacgctgata ttgtcaatga agtcaagggt ggttatatca
     1021 tcaaggtcga tggaaaatat tatgtctacc tgaaagatgc agctcatgct gataatgttc
     1081 gaactaaaga tgaaatcaat cgtcaaaaac aagaacatgt caaagataat gagaaggtta
     1141 actctaatgt tgctgtagca aggtctcagg gacgatatac gacaaatgat ggttatgtct
     1201 ttaatctagc tgatattatc gaagatacgg gtaatgctta tatcgttcct catggaggtc
     1261 actatcacta cattcccaaa agcgatttat ctgctagtga attagcagca gctaaagcac
     1321 atctggctgg aaaaaatatg caaccgagtc agttaagcta ttcttcaaca gctagtgaca
     1381 ataacacgca atctgtagca aaaggatcaa ctagcaagcc agcaaataaa tctgaaaatc
     1441 tccagagtct tttgaaggaa ctctatgatt cacctagcgc ccaacgttac agtgaatcag
     1501 atggcctggt ctttgaccct gctaagatta tcagtcgtac accaaatgga gttgcgattc
     1561 cgcatggcga ccattaccac tttattcctt acagcaagct ttctgcctta gaagaaaaga
     1621 ttgccagaat ggtgcctatc agtggaactg gttctacagt ttctacaaat gcaaaaccta
     1681 atgaagtagt gtctagtcta ggcagtcttt caagcaatcc ttcttcttta acgacaagta
     1741 aggagctctc ttcagcatct gatggttata tttttaatcc aaaagatatc gttgaagaaa
     1801 cggctacagc ttatattgta agacatggtg atcatttcca ttacattcca aaatcaaatc
     1861 aaattgggca accgactctt ccaaacaata gtctagcaac accttctcca tctcttccaa
     1921 tcaatccagg aacttcacat gagaaacatg aagaagatgg atacggattt gatgctaatc
     1981 gtattatcgc tgaagatgaa tcaggttttg tcatgagtca cggagaccac aatcattatt
     2041 tcttcaagaa ggacttgaca gaagagcaaa ttaaggctgc gcaaaaacat ttagaggaag
     2101 ttaaaactag tcataatgga ttagattctt tgtcatctca tgaacaggat tatccaagta
     2161 atgccaaaga aatgaaagat ttagataaaa aaatcgaaga aaaaattgct ggcattatga
     2221 aacaatatgg tgtcaaacgt gaaagtattg tcgtgaataa agaaaaaaat gcgattattt
     2281 atccgcatgg agatcaccat catgcagatc cgattgatga acataaaccg gttggaattg
     2341 gtcattctca cagtaactat gaactgttta aacccgaaga aggagttgct aaaaaagaag
     2401 ggaataaagt ttatactgga gaagaattaa cgaatgttgt taatttgtta aaaaatagta
     2461 cgtttaataa tcaaaacttt actctagcca atggtcaaaa acgcgtttct tttagttttc
     2521 cgcctgaatt ggagaaaaaa ttaggtatca atatgctagt aaaattaata acaccagatg
     2581 gaaaagtatt ggagaaagta tctggtaaag tatttggaga aggagtaggg aatattgcaa
     2641 actttgaatt agatcaacct tatttaccag gacaaacatt taagtatact atcgcttcaa
     2701 aagattatcc agaagtaagt tatgatggta catttacagt tccaacctct ttagcttaca
     2761 aaatggccag tcaaacgatt ttctatcctt tccatgcagg ggatacttat ttaagagtga
     2821 accctcaatt tgcagtgcct aaaggaactg atgctttagt cagagtgttt gatgaatttc
     2881 atggaaatgc ttatttagaa aataactata aagttggtga aattaaatta ccgattccga
     2941 aattaaacca aggaacaacc agaacggccg gaaataaaat tcctgtaacc ttcatggcaa
     3001 atgcttattt ggacaatcaa tcgacttata ttgtggaagt acctatcttg gaaaaagaaa
     3061 atcaaactga taaaccaagt attctaccac aatttaaaag gaataaagca caagaaaact
     3121 caaaacttga tgaaaaggta gaagaaccaa agactagtga gaaggtagaa aaagaaaaac
     3181 tttctgaaac tgggaatagt actagtaatt caacgttaga agaagttcct acagtggatc
     3241 ctgtacaaga aaaagtagca aaatttgctg aaagttatgg gatgaagcta gaaaatgtct
     3301 tgtttaatat ggacggaaca attgaattat atttaccatc gggagaagtc attaaaaaga
     3361 atatggcaga ttttacagga gaagcacctc aaggaaatgg tgaaaataaa ccatctgaaa
     3421 atggaaaagt atctactgga acagttgaga accaaccaac agaaaataaa ccagcagatt
     3481 ctttaccaga ggcaccaaac gaaaaacctg taaaaccaga aaactcaacg gataatggaa
     3541 tgttgaatcc agaagggaat gtggggagtg accctatgtt agatccagca ttagaggaag
     3601 ctccagcagt agatcctgta caagaaaaat tagaaaaatt tacagctagt tacggattag
     3661 gtttagatag tgttatattc aatatggatg gaacgattga attaagattg ccaagtggag
     3721 aagtgataaa aaagaattta tctgatctca tagcgtaagg aatagcagta gaaaaagtct
     3781 gaatcaaaaa tgaagttctc tcaaaagtt
//
LOCUS       DAVLRT010000114         3786 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLRT010000114 DAVLRT010000000
VERSION     DAVLRT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.114"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            153..380
                     /locus_tag="VXM85_001960"
     CDS             153..380
                     /locus_tag="VXM85_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346474.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            668..3565
                     /locus_tag="VXM85_001961"
     CDS             668..3565
                     /locus_tag="VXM85_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV1346475.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDKVVISNLRDNPEAKD
                     PAAPSPLNIDMVLVQDPTTKRIFSIYDMFPEGRAVFGMPKTPEKAYEKIGDKTYQILY
                     KQGESGHYTVRENGEVYNAQNQKTDYRVVVNPTEPGYRDKGNLYKGQELIGNIYFAHS
                     TKNPFRVANTSYLWMSYSDDDGKTWSAPRDITPGLRKDWMKFLGTGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1312 a          603 c          854 g         1017 t
ORIGIN      
        1 aaaaatgagg tataataaat ttactaacac ttataaaaag tgatagaatc tatctttatg
       61 tatatttaaa gatagattgc tgtaaaaata gtagtagcta tgcgaaataa cagatagaga
      121 gaagggattg aagcttagaa aaggggaata atatgatatt taaggcattc aagacaaaaa
      181 agcagagaaa aagacaagtt gaactacttt tgacagtttt ttttgacagt tttctgattg
      241 atttatttct tcacttattt gggattgtcc cctttaagct ggataagatt ctgattgtga
      301 gcttgattat atttcccatt atttctacaa gtatttatgc ttatgaaaag ctatttgaaa
      361 aagtgttcga taaggattga gcaggaagta tggtataaat agcataagct gatgtccatc
      421 atttgcttat aaagagatat tttagtttaa ttgcagcggt gtcctggtag ataaactaga
      481 ttggcaggag tctgattgga gaaaggagag gggaaaattg gcaccaattt gagatagttt
      541 gtttagttca tttttgtcat ttaaatgaac tgtagtaaaa gaaagttaat aaaagacaaa
      601 ctaagtgcat tttctggagt aaatgtctta tttcagaaat cgggatatag atatagagag
      661 gatcagtatg aatcggagtg ttcaagaacg taagtgtcgt tatagcatta ggaaactatc
      721 ggtaggagcg gtttctatga ttgtaggagc agtggtattt ggaacgtctc ctgttttagc
      781 tcaagaaggg gcaagtgagc aacctctggc aaatgaaact caactttcgg gggagagctc
      841 aaccctaact gatacagaaa agagccagcc ttcttcagag actgaacttt ctggcaataa
      901 gcaagaacaa gaaaggaaag ataagcaaga agaaaaaatt ccaagagatt actatgcacg
      961 agatttggaa aatgtcgaaa cagtgataga aaaagaagat gttgaaacca atgcttcaaa
     1021 tggtcagaga gttgatttat caagtgaact agataaacta aagaaacttg aaaacgcaac
     1081 agttcacatg gagtttaagc cagatgccaa ggctccagca ttctataatc tcttttctgt
     1141 gtcaagtgct actaaaaaag atgagtactt cactatggca gtttacaata atactgctac
     1201 tctagagggg cgtggttcgg atgggaaaca gttttacaat aattacaacg atgcaccctt
     1261 aaaagttaaa ccaggtcagt ggaattctgt gactttcaca gttgaaaaac cgacagcaga
     1321 attgccaaat ggtcgagttc gcctgtatgt taatggtgtt ttatctcgaa caagtctgaa
     1381 atctggcaat ttcattaaag atatgccaga tgtaacgcat gtgcaaatcg gagcaaccaa
     1441 gcgtgccaac aatacggttt gggggtcaaa tctacagatt aggaatctca ctgtgtataa
     1501 tcgtgcttta acaccagaag aggtacaaaa acgtagtcaa ctttttaaac gctcagattt
     1561 agaaaaaaaa ctacctgaag gagcggcttt aacagagaaa acggacatat tcgaaagcgg
     1621 gcgtaacggt aaaccaaata aagatggaat caagagttat cgtattccag cacttctcaa
     1681 gacagataaa ggaactttga tcgcaggtgc agatgaacgc cgtctccatt cgagtgactg
     1741 gggtgatatc ggtatggtca tcagacgtag tgaagataat ggaaagacat ggggagataa
     1801 ggtggttatc tccaatcttc gagataatcc tgaagctaaa gatcctgctg cgccatcgcc
     1861 tctaaatatt gatatggttt tggttcaaga cccgacaaca aagagaatct tctcaattta
     1921 tgatatgttc ccagaaggtc gagcagtttt tggaatgcca aaaacacctg aaaaagctta
     1981 tgaaaagata ggggataaaa cttatcaaat cttgtataaa caaggagagt ctggtcatta
     2041 tactgttcgt gagaatggag aagtgtataa tgcacaaaat caaaagacgg attatcgtgt
     2101 tgtagtgaat ccaacagaac ctggctatag agataaagga aatctttaca aaggtcagga
     2161 attgattgga aatatctatt ttgcacacag tacaaaaaat ccatttagag tagccaatac
     2221 gagctatcta tggatgtcat atagtgacga tgatggtaaa acttggtctg caccgagaga
     2281 cattactcca ggtcttcgca aggattggat gaagttccta ggaacaggtc ctggaacagg
     2341 aattgtactt cggaatgggc ctcacaaggg acggattttg ataccggttt atacgactaa
     2401 taatgtatct cacttaaatg gctcgcaatc ttctcgtgtc atctattcag atgatcatgg
     2461 aaaaacttgg catgctggag aagcggtcaa cgataaccgt caggtagacg gtcaaaagat
     2521 ccactcttct acgatgaaca atgaacgtgc gcaaaataca gaatcaacgg tggtacaact
     2581 aaacaatgga gatgttaaac tctttatgcg tggtttgact ggagatcttc aggttgctac
     2641 aagtaaagac ggaggagtga cttgggagaa ggatatcaaa cgttatccac aggttaaaga
     2701 tgtctatgtt caaatgtctg ctatccatac gatgcacgaa ggaaaagaat acatcatcct
     2761 cagtaatgca ggtggaccga aacgtgaaaa tgggatggtc cacttggcac gtgtcgaaga
     2821 aaatggtgag ttgacttggc tcaaacacaa tccaattcaa aaaggagagt ttgcctataa
     2881 ttcgctccaa gaattaggaa atggggagta tggtatcttg tatgaacata ctgaaaaagg
     2941 acaaaatgcc tataccctat catttagaaa atttaattgg gactttttga gcaaagatct
     3001 gatttctcct accgaagcga aagtgaagcg aactagagag atgggcaaag gagagatggg
     3061 caaaggagtt attggcttgg agttcgactc agaagtattg gtcaacaagg ctccaaccct
     3121 tcaattggca aatggtaaaa cagcgacttt cctaacccag tatgatagca agaccttgtt
     3181 atttgcagta gataaggaag atatcggaca ggaaattatt ggtatagcta aaggaagcat
     3241 cgaaagtatg cataatcttc ctgtaaatct agcaggtgcc agagttcctg gcggagtaaa
     3301 tggtagcaaa gcagcggtgc atgaagttcc agaatttaca gggggagtta atggtacaga
     3361 gccagctgtt catgaaatcg cagagtataa gggatctgat tcgcttgtaa ctcttactac
     3421 aaaagaagat tatacttaca aagctcctct tgctcagcag gcacttcctg aaacaggaaa
     3481 caaggagagt gacctcctag cttcactagg actaacagct ttcttccttg gtctgtttac
     3541 gctagggaaa aagagagaac aataagagaa gaattctaaa catttgattt tgtaaaaata
     3601 gaaggagata gcaggttttc aagcctgcta tctttttttg atgacattca agctgatacg
     3661 aaatcataag aggtatgaaa ctactttcag ggtagtctgt tctataaaat atagtagatt
     3721 gaaataagat gtgaacaact ctaccaggaa agtcaaatta atttatagaa ttattttagc
     3781 agtcaa
//
LOCUS       DAVLRT010000115         3738 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLRT010000115 DAVLRT010000000
VERSION     DAVLRT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.115"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..494)
                     /locus_tag="VXM85_001962"
     CDS             complement(<1..494)
                     /locus_tag="VXM85_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1346476.1"
                     /translation="MLSLDQIHLLLNTPEDEFHDFKQKWHHSKTELVRDILNFVNTSH
                     HEDCYIIFGIDNITLDIIGVNNDDNRRNEEDLTDLLHKLFISTNNQIKISIQTETIDN
                     KEIDILIIHDTDKVPVFLTKDYKPKKDTALPKGLIYAINGSINTPKDSSAPFELINEL
                     FQKF"
     gene            856..1212
                     /locus_tag="VXM85_001963"
     CDS             856..1212
                     /locus_tag="VXM85_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346477.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
     gene            1217..1582
                     /locus_tag="VXM85_001964"
     CDS             1217..1582
                     /locus_tag="VXM85_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1346478.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLIPGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            1569..3392
                     /locus_tag="VXM85_001965"
     CDS             1569..3392
                     /locus_tag="VXM85_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1346479.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGIDFQK"
     gene            3506..>3738
                     /locus_tag="VXM85_001966"
     CDS             3506..>3738
                     /locus_tag="VXM85_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1346480.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEEVNELSEF"
BASE COUNT         1285 a          584 c          754 g         1115 t
ORIGIN      
        1 ttaaactttt gaaacaactc atttattaac tcaaaaggag cagaggagtc tttaggggta
       61 tttatagagc catttatagc atatattaat ccttttggta atgcagtatc tttcttaggc
      121 ttataatctt ttgttaaaaa tacaggaact ttatctgtat catgaataat taagatgtca
      181 atttctttgt tgtctatagt ttcagtttgt atgctaattt taatttgatt atttgttgat
      241 ataaagagtt tatgtagtaa atctgttaga tcttcttcat ttcttctatt atcatcattg
      301 tttacaccga ttatatctaa agtgatatta tcaattccaa agatgatata acaatcttcg
      361 tgatgtgatg tattgacaaa atttaagata tcacgcacca attcagtttt tgaatgatgc
      421 catttttgtt taaaatcatg aaactcatct tcgggtgtat tcaatagtaa gtgtatttga
      481 tctagactca gcatttttat ttctccaaat attagtttac agaaatttta tcatagaatc
      541 agagaaagtc aatcataact atattagctg tgataccttt ttcaaatctt ccctgttatc
      601 ctaatatcat tcatagaaaa tcaatttttg attttctatg ggggtttggg ggcgaagtca
      661 ccaagttatc ttatcgttgg ctgtcaaaac tagaaagttt tgataggctg gcgatatgat
      721 ttttgagata ttgtggacac aatatctgag ctcgcaaagc cttacaagaa tgaaaatcag
      781 ttgttggaaa agtgtactga cattgtatgg tagctcacat tgtcagtaca agtattttgg
      841 aaaggaagta gcagtatgaa acgagatgtg cgtgatattc ggaaacaatt tcgtttaaca
      901 gaagcagaag aaaagcaaat tctagctttg atgagagagc ggggagagac taatttctct
      961 gattttcttc gtaaaagttt actttcctct gatttacaaa aacagatgga gacatggttt
     1021 gccctctggc aatcccaaaa actagaacaa atcagtcgtg acgttcatga agttttaatc
     1081 ttggcacagt cagaacgtca agtcacccaa gagcatgtat ctattctctt aacgtgcgtg
     1141 caggaattga ttcaagaggt tgcaaacacc atacccctca gtaaagaatt tcgtgagaag
     1201 tacatgaggt aagcacatgg aacatcgtta ccgaaccaat ctcaagaaag tgtttttgtc
     1261 tgatagtgag ttgaaccaac taaatataaa tatcgatcaa agtggttgta aatccttttc
     1321 tgaatatgcg agacgaactc tactcgatcc tggtatgaat tttatcacga ttgacacaaa
     1381 cggttaccaa gatttagtgt ttgagttaaa gaggattggc aataatatca accagattgc
     1441 tcgaagtgtt aatcaatctc agttaattcc tggtgaagaa ttgcaggagt tgaaaaaagg
     1501 aattggtgaa ttgataaaag aagttgataa ggaatttaat ctgcaagcgc agaagctaaa
     1561 ggagttccat ggtcatcact aaacactttg ccattcacgg aaagagttac cgcagaaagc
     1621 ttatcaagta cattctcaat cctgagaaaa ccaataatct tgccttggtg tcggactatg
     1681 gcatgaagaa ttttctggac tttcctagct atgaggaaat ggtgcagatg tatcatgaaa
     1741 atttcatcag caacgatacg ctttacgatt ttcgccacga caggatggaa gaaaatcaac
     1801 gaaaaataca cgctcaccac atcattcagt ctttctcgcc agaggatcat ctcactcctg
     1861 aacaaatcaa tcggataggt tatgagactg tgaaggaatt aactggtggc aaatttcgtt
     1921 ttatcgttgc gacccatgtt gataaagacc acctgcacaa tcacatcatt atcaattcag
     1981 tagatagcaa ttctgacaaa aagctcaagt gggactacaa ggtggagcga aatcttcgca
     2041 tgatttctga ccgtttttct aaaatcgcag gtgctaaaat cattgagaac cgctattctc
     2101 accagcggta tgaagtctat cgtaagacta atcacaagta tgaactcaag cagcgactct
     2161 attttttgat ggaacattct agggactttg aggatttcaa aaagaatgct ccgctactac
     2221 atgtggagat ggatttccgt cacaagcatg ccaccttttt tattacggac tcaactatga
     2281 aacaggtggt gcgtggcaag caactcaatc gcaagcagcc ttacacagaa gaatttttta
     2341 agaactactt tgccaaaaga gaaatagaaa gtctcatgga atttttattg ctgaaagttg
     2401 agaatatgga tgatttactt cagaaagcaa aactttttgg actaactatc aatcctaaac
     2461 aaaagcatgt ttcttttcaa tttgcaggag tggaggtaaa ggagacagag ctagaccaga
     2521 aaaatcttta tgatgtagag tttttccaag attattttaa aaatagaaaa gattggcaag
     2581 ctccagaaac tgaggatttc gttcaacttt atcaagaaga aaagttatcc aaagaaaaag
     2641 aacttccaag cgatgagaag ttctgggagt cctatcaaga gttcaagagt aacagagatg
     2701 ccgttcatga atttgaggtg gagttgtcac tcaatcaaat tgaaaaagta gtggatgatg
     2761 gaatttacgt caaggtcaag tttggtattc gtcaggaggg acttatcttt gtgccgaaca
     2821 tgcagcttga tatggaagag gataaggtga aggttttcat cagggaaacc agctcctact
     2881 atgtctacca caaagacgct gccgagaaaa attgttatat gaaaggtcga accttaatta
     2941 gacagttcag ctatgaaaat caaaccattc cattacgcag aaaagcgaca gtcgatatga
     3001 ttaaagagaa gattgcggaa gtggatgctt tgattgaact ggaagtagaa aatcaatctt
     3061 atgtcacgat taaagatgag ttagtgcatg aactagcagc gtctgaatta agaatcaatg
     3121 agttgcaaga acgaatgtca accttgaatc aagtagcaga atatctactg gcttcagttg
     3181 aaagtaagca agaaatgaaa ttaaatcttt caaaactgaa tataactgag aatatcagtg
     3241 ctaatattgt tgagaaaaaa ttgaagagcc tggggaatca actggaattg gaaaggggca
     3301 ggtatgaaaa gatggtagtc aggctagata aatttataaa tcgcttaaat acaggacttt
     3361 caaaaggaga tgggatagat tttcaaaaat aggaatctag ccaataatta tatacaatgt
     3421 gtatcttttg taaaatatat ttgaaattta gatttaatca tgttacaatt aacattgaaa
     3481 tcaaatagaa aaggaagatg caaacgtggg tacactatta gcaacaagat taaaaaatag
     3541 acgaaaagaa ttaaaaatgt ctcagcgaga attagctgag ggtatctgta aacaggggca
     3601 aattagtcga ttagagagtg gagagttcac ccctggagcg gattttttac atgcccttgc
     3661 taagaaatta aaagttagta tggactactt ttttgatgag caggttgtag aggaagtcaa
     3721 tgagttatca gagtttaa
//
LOCUS       DAVLRT010000116         3737 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLRT010000116 DAVLRT010000000
VERSION     DAVLRT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.116"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            96..983
                     /locus_tag="VXM85_001967"
     CDS             96..983
                     /locus_tag="VXM85_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEV1346481.1"
                     /translation="MLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIAST
                     LYFYLSPISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATAL
                     MPPLCTVGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDS
                     KMRRILLLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHT
                     LKLTISGNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKD
                     KEAKEKEKANKFDTESEEQ"
     gene            1150..3237
                     /gene="topA"
                     /locus_tag="VXM85_001968"
     CDS             1150..3237
                     /gene="topA"
                     /locus_tag="VXM85_001968"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1346482.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMAVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3343..3702
                     /locus_tag="VXM85_001969"
     CDS             3343..3702
                     /locus_tag="VXM85_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1346483.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
BASE COUNT         1132 a          680 c          797 g         1128 t
ORIGIN      
        1 aactttccag aagtgattta gcgattttac tatgtgccat gcttatcgcc tctatcggat
       61 taaatatgga ttcgactccc gtgattattg gagccatgtt aatctctcct ttgatgacac
      121 ctattctggg agtggggctc tctctagcta tatttgattt taaattgtta agaaaatctt
      181 ttaaaatatt agctattcaa attcttgcca gtctaatagc ttcaacactt tatttttatc
      241 tttctcccat ttcgtatgct agttcggaga ttgttgctag aacctctccg actatttggg
      301 atgttctcat tgcttttgta ggagggatag caggtattat tggtgctagg aaaaaagaga
      361 ccaataatat tgttcctggt gttgctattg caaccgcctt gatgcctcct ctttgtacag
      421 taggttatgc tattgcttct gctaatctaa aatttatcat aggctcctct tacctattcc
      481 tcatcaattg tagctttatt gtcattgcga cttatatagg tgttaggttg atgatggtta
      541 agaaacatta ttttaaagat aacgaagaag actctaaaat gcgtaggatt ttgcttctag
      601 ttgctgtttt gctgatgatt ccgagtttca tctctgcaac gactttagtg agagaaacgt
      661 tgaaaaaaga gtcccttaag aaatttatat cagagcagtt tcaggggtat aatattttga
      721 aaaaaaccta ttctaaaaag actcataccc taaagctaac catttcagga aattatttga
      781 cagaagaaga actcgatatg atttcaagta agagaggtga ctatggttta agtgatgttt
      841 ctgttcaagt ttcacaattg tctgattcag aacaacttag taaggaagag ctggtggagt
      901 atttcttcca gtatatcaag gataaggaag caaaagaaaa ggagaaagcc aataaatttg
      961 atacagagtc tgaggagcaa taatttcttg agaatagctg gtttctctcg tgagtcttct
     1021 atgtatatca aaggaagact gaggttttaa gtataaaact tttcttctat tatagtagct
     1081 aagtcttgac agggttcaaa aaatggttta cactttataa agtttattac tttgaaaagg
     1141 tgtgatactg tggctacggc aacaaaaaag aaaaaatcaa cagttaaaaa aaatctagtc
     1201 atcgtggagt cgcctgctaa ggccaagacg attgaaaaat atctaggcag aaactacaag
     1261 gttttagcca gtgtcgggca tatccgtgat ttgaagaaat ccagtatgtc cgtcgatatt
     1321 gaaaataatt atgaaccgca atatatcaat atccgaggaa aaggccctct tatcaatgac
     1381 ttgaaaaaag aagctaaaaa agctaataaa gtttttctcg cgagtgaccc ggaccgtgaa
     1441 ggagaagcga tttcttggca tttggcccat attctcaact tggatgaaaa tgatgccaac
     1501 cgtgtggtct tcaatgaaat caccaaggat gcagtcaaaa atgcttttaa agaacctcgt
     1561 aagatcgata tggacttggt cgatgcccaa caagctcgtc ggatcttgga tcgcttggta
     1621 gggtattcga tttcgcctat tttgtggaag aaggtcaaga agggcttgtc agcaggtcgc
     1681 gttcagtcca ttgcccttaa actcatcatt gaccgtgaaa atgaaatcaa tgccttccag
     1741 ccagaagaat actggacagt tgatgctgtc tttaaaaagg gaaccaaaca atttcatgct
     1801 tccttctatg gagtagatgg taaaaagatg aaactgacca gcaataacga agtcaaggaa
     1861 gtcttgtctc gtctgacgag taaagacttt tcagtagatc aggtggataa gaaagagcgt
     1921 aagcgcaatg ctcctttacc ctataccact tcatctatgc agatggatgc tgccaataaa
     1981 atcaatttcc gtactcgaaa aaccatgatg gttgcccaac agctctatga aggaattaat
     2041 atcggttctg gtgttcaagg tttgattacc tatatgcgta ccgattcgac tcgtatcagt
     2101 cctgtagcgc aaaatgaggc ggcaagcttc attacggatc gttttggtag caagtattct
     2161 aagcacggta gcaaggtcaa aaacgcatca ggtgctcagg atgcccatga ggctattcgt
     2221 ccgtcgagtg tctttaatac accagaaagc atcgctaagt atctggacaa ggatcagctc
     2281 aagctatata cccttatctg gaatcgtttt gtggctagcc agatgacagc ggccgttttt
     2341 gataccatgg ctgttaaatt gtctcaaaat ggagttcaat ttgctgccaa tggtagtcag
     2401 gttaagtttg atggttatct tgccatttat aatgattctg acaagaataa aatgctacca
     2461 gatatggctg ttggagatgt ggtcaaacag gttaatagca aaccagagca acatttcacc
     2521 caaccgcctg cccgttattc tgaagcaaca ctgattaaaa ccttagagga aaatggggtt
     2581 ggacgtccat caacctatgc gccaaccatt gaaaccattc agaaacgtta ttatgttcgc
     2641 ctggcagcca aacgttttga accgacagag ttgggagaaa ttgtcaataa gctcatcgtt
     2701 gaatatttcc cagatatcgt aaacgtgacc ttcacagctg aaatggaagg taaactggat
     2761 gatgtcgaag ttggaaaaga gcagtggcga cgggtcattg atgcctttta caaaccattc
     2821 tctaaagaag ttgccaaggc tgaagaagaa atggaaaaaa tccagattaa ggatgaacca
     2881 gctggatttg actgtgaagt gtgtggcagt ccaatggtca ttaaacttgg tcgttttggt
     2941 aaattctacg cttgtagcaa tttcccagat tgccgtcata cccaagcaat cgtgaaagag
     3001 attggtgttg agtgtccaag ctgtcatcag ggacaaatta ttgagcgaaa aaccaagcgt
     3061 aatcgccttt tctatggttg caatcgctat ccagaatgtg aatttacctc ttgggacaag
     3121 cctgttggtc gtgactgtcc aaaatgtggc aacttcctca tggagaaaaa agtccgtggt
     3181 ggtggcaagc aggttgtttg tagcaaaggc gactacgagg aagaaaagat taaataagag
     3241 gaggggcccg aaagtaaatt tcaggctcct tttggttaga acttgacaaa atacatcatt
     3301 ttatgcgaaa ctagaagaag attattttaa ctaggagaag ttatgcgtct tatctatctg
     3361 ataattggtt ttttatcact ggccttggct attgttgggg ttgttttacc cttgttgccg
     3421 acaacgcctt tccttttgtt gtctattgct tgtttctcca gaagttccaa gcgtttcgaa
     3481 gactggcttt atcataccaa gctctatcaa acatatgtag ctgattttcg cgagactaag
     3541 tctattacgc gtgaacgaaa gaaaaaaatc atcgtctcta tctacgtctt gatgggaatt
     3601 tctatttatt ttgcacctct tttaccagtc aaaatcggtc tgggtgcttt gaccatcttt
     3661 atcacttatt atcttttcaa ggtcattcca gacaaagaat agttaaaaca gtagttattt
     3721 gccttgataa aattgaa
//
LOCUS       DAVLRT010000117         3689 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLRT010000117 DAVLRT010000000
VERSION     DAVLRT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.117"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..725
                     /locus_tag="VXM85_001970"
     CDS             <1..725
                     /locus_tag="VXM85_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1346484.1"
                     /translation="PIVDKIIQTHNIKRIIFLGDYVDLHGQTNNIQLYAKDLTFLYSW
                     KIDKEQNGIDVINLIGNHDAYYLLGEQAPFSIQNLEVFFAVKELLQDLKLQIAYQLDD
                     YLVSHAGFNLIFDPKEWHFNLYTKEYEEELDILAYTIGPMRGGKALGGSPLWAHFREL
                     ELLPNQDFPKQIVGHTPKESIDISKNVIGIDTFSLYIDKDNKYQFIGNGDLLVYDQGH
                     LEIISTNWATDETLKQLSKPQI"
     gene            909..1691
                     /locus_tag="VXM85_001971"
     CDS             909..1691
                     /locus_tag="VXM85_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEV1346485.1"
                     /translation="MTSYNKKIVRTSSYIEIWEYEKPIFSKGKSILDTNTENKEKAKR
                     RTFDELTPLEQEQRLERMKKTRLEAKWNLLRLVDCNFDDTTSFLTLTTKENISDRNHF
                     KDSLKTFIKRFNYHIFNTKKSKLKYIAVLERQKRKAWHSHILLFSVPYIPHKQLLELW
                     GHGAVWINKVDVDSKENRGRYVTKYFEKGIGQELLENFGKQAYFSSRNLKKPDEDKFY
                     TYEDFNYDSSVVLYETEYTSKVYKYGQYFDNHVKYKKIRLDE"
     gene            1705..1908
                     /locus_tag="VXM85_001972"
     CDS             1705..1908
                     /locus_tag="VXM85_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEV1346486.1"
                     /translation="MIATVTKNDLIALGFFEGTSKRIIRQGKELLITRGFTVYQNKRI
                     GTIPATIATELLVFDVQNSTLKE"
     gene            1912..3045
                     /locus_tag="VXM85_001973"
     CDS             1912..3045
                     /locus_tag="VXM85_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1346487.1"
                     /translation="MAIRKGKDGNWVVDISNGFDPITLKQRRLVRKGYKTKKEAIEAE
                     HYLRSVELKERFYGAKITIPILYELLKEEDSINHRKASYINTQDNNYNKHIKDYFSMV
                     DNVGKLTYEDIYQFREHLRQKVAKNSDKPLSTNTINKIMILLKKIFDVGLRKGYYSSN
                     PAKLIKKLPIEKTKMQFWTVKEFQKFLTLFEPEEYNIKLLFTVLFFTGLRLGEALALT
                     WQDIDFSSNTIHITKSVYVNKGVTHISTTKTKAETRRIVINKKLSQELQDWKKRQKHL
                     LEQFTNNSLTLQVFQNSPITITKNAIEKQYKKIIERDSTLKKIRIHDFRHSHASLLIN
                     QGEDYLVVKERLGHASITTTIDTYSHLYPSKQKDLADKLDDLL"
     gene            3086..3523
                     /locus_tag="VXM85_001974"
     CDS             3086..3523
                     /locus_tag="VXM85_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1346488.1"
                     /translation="MDTSQVIKAHEVAKKAHFGQIDRAGIDYIKHPETVASFVATDEE
                     KAVAYLHDVIEDTSLTLLDLKKEGFSKNIIEAVDILTKKKGQDYQSYLNLVKKNELAR
                     VVKLADLRHNSDLTRLPLITEKDLERNKKYSSAIRFLRAKKKS"
BASE COUNT         1434 a          610 c          608 g         1037 t
ORIGIN      
        1 tacccattgt tgacaaaatt atccaaacac ataacattaa gagaatcatt tttcttggtg
       61 attatgttga tttacatggt caaactaaca atatccaact ttatgccaaa gacctcactt
      121 ttttatactc ttggaaaata gataaagagc aaaacggaat agatgtaatt aatcttatag
      181 gtaatcatga tgcttattat ttactgggag aacaagctcc tttctcaatt caaaatttag
      241 aagtgttttt cgcagtaaaa gaattgttgc aggatttgaa attacaaata gcttatcaac
      301 tagatgatta tttagtcagt cacgctggtt tcaatctgat atttgaccca aaagaatggc
      361 attttaacct ttataccaaa gagtatgaag aagaattaga tatcctagct tatacaatag
      421 gaccaatgcg tggtgggaaa gctcttgggg gttctccttt atgggcacat tttagagaat
      481 tagaattgct tcccaaccaa gactttccta aacaaattgt tggacatacc cctaaagaat
      541 ctattgatat ttctaaaaat gttattggta tagatacttt ttcattatat attgacaaag
      601 ataataaata tcaatttatt ggtaacggag atttgctagt ctatgaccaa ggccatcttg
      661 aaatcatttc aaccaactgg gctacagacg aaacattaaa acaattatct aaaccacaaa
      721 tttaactact acttctcatc atgtgactaa gcgaaactgc tgactggagg tcagcaagct
      781 ggaagcgtgt ttagcggagc gatgcttaga aacacatgat agaagtgtgg aggagctttg
      841 ctcctcctag tttatttatt atatagacaa tcttttggcg aaagccaacc taaaaggaac
      901 tattttttat gacctcatat aataaaaaaa tagtccgcac ttcttcctat attgaaattt
      961 gggaatatga aaagccaata ttttcaaaag gaaaatctat tttagatact aatactgaaa
     1021 acaaggaaaa agcaaaacgc agaacatttg atgaactaac acccctagaa caagaacaac
     1081 gtcttgaacg tatgaagaag actcgcttag aagccaagtg gaacttactc cgtctagttg
     1141 attgtaattt tgatgataca acttcttttc ttacactgac cactaaggaa aatatcagcg
     1201 atagaaacca cttcaaagac tcactcaaaa catttattaa acgctttaac taccacattt
     1261 ttaataccaa gaaaagtaaa ttaaaatata tagcagtatt agagaggcaa aaacgtaaag
     1321 catggcatag tcatattttg ctattcagtg ttccctatat tccacataag caattattag
     1381 aactttgggg gcatggtgca gtatggataa ataaagttga cgtcgacagc aaagaaaatc
     1441 gcggtcgata tgtcacaaag tattttgaaa aaggtattgg acaagaattg ttggaaaact
     1501 tcggaaagca agcctatttt tcatcacgca acttgaaaaa gcctgatgaa gataaatttt
     1561 acacttatga agattttaac tatgatagtt cagtcgtgct atatgaaacg gaatacacta
     1621 gtaaggtcta taaatacggt cagtatttcg ataatcatgt aaagtacaag aaaataagac
     1681 tagatgaata aaggagtaag tcacatgata gcaactgtta ctaagaatga cctaattgcc
     1741 ctcggtttct ttgagggaac atctaaacgt attatccgcc aagggaaaga gctactgata
     1801 acccgaggct ttacagtcta tcagaacaag cgaattggta ctatacctgc tactattgct
     1861 accgaattac tcgtttttga tgttcaaaac agtaccctca aggagtaaaa tatggcaatt
     1921 agaaaaggta aagacggtaa ttgggtagtt gatattagca acggttttga cccaataaca
     1981 ctaaagcaac gtagacttgt cagaaagggc tataagacga aaaaagaagc tattgaagca
     2041 gaacactact tacgaagtgt agagttaaaa gaacgctttt atggagctaa aataactatc
     2101 cccatactat atgaactact taaggaggaa gatagtataa atcatcgaaa agcaagctat
     2161 atcaatacac aagataataa ttacaacaaa cacatcaaag attatttttc tatggtcgat
     2221 aatgtcggaa aactaaccta tgaagacatc taccaattta gagaacatct taggcaaaaa
     2281 gtagctaaaa attctgataa accgttaagc acaaatacta ttaacaaaat catgatttta
     2341 ctcaaaaaaa tttttgatgt tggtttgcga aaaggctatt actcctccaa tccagcaaaa
     2401 ttaataaaaa agttgccaat tgaaaaaaca aaaatgcaat tttggacagt caaagaattt
     2461 cagaagtttt tgacactatt tgaacccgaa gaatacaata tcaaactact ctttactgtt
     2521 ctattcttta caggattaag acttggggaa gctcttgccc taacatggca agacatcgac
     2581 ttttcaagta atacaattca tatcacgaag tctgtctatg ttaataaagg agtaactcat
     2641 atcagcacaa caaaaactaa agcagaaaca agaagaattg ttatcaataa aaaattaagc
     2701 caagaattac aagattggaa aaagcgacaa aaacatctat tagagcaatt taccaacaat
     2761 agcctaacat tacaagtgtt tcaaaatagt cctatcacaa ttacaaaaaa tgctattgaa
     2821 aagcagtaca agaaaatcat tgaacgtgat agcacactaa aaaagatacg catacacgat
     2881 tttaggcact cacacgcttc cctgctgatt aaccaagggg aagattatct agttgtaaaa
     2941 gagcgactag gacatgcttc tataacaacc acgattgata cctactctca cttgtaccca
     3001 agtaagcaaa aggatttagc tgataaacta gatgatttac tataaagtaa ttgagtttta
     3061 taaatggaaa gtttataatt aaatcatgga tacatcacaa gtaataaaag cacatgaagt
     3121 agctaaaaaa gcacactttg gacaaattga tagagcagga attgactaca ttaaacaccc
     3181 tgaaacagta gctagttttg tcgcaactga tgaagagaaa gctgtcgcct atctacatga
     3241 tgtaattgaa gatacttcac taaccctact agacctaaaa aaggaaggtt tttcaaaaaa
     3301 cattatagaa gccgtcgata tactcacaaa gaaaaaagga caagattatc aatcttatct
     3361 caatctagta aagaaaaatg aactcgctag agttgtaaag ttggcggatt tacggcataa
     3421 ttcagattta acaagactac cattaattac tgaaaaagac ttagagagaa ataagaaata
     3481 cagttctgct attagatttt taagagcaaa gaaaaaaagt tagtaactaa actataaaat
     3541 tttataaaga gaaaacaaaa gacaaggctt ataaactttg tctttattac tatactggcg
     3601 gtcggtctcg aactggcaca tccttgcaga tactacaatt ttattaaaat aaataactat
     3661 aaaaagtgaa attttttatt gagaaactt
//
LOCUS       DAVLRT010000118         3576 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLRT010000118 DAVLRT010000000
VERSION     DAVLRT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.118"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(76..1491)
                     /locus_tag="VXM85_001975"
     CDS             complement(76..1491)
                     /locus_tag="VXM85_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1346489.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTLNRY
                     KKMSFTWYKEVIESNGESLFK"
     gene            complement(1504..3342)
                     /locus_tag="VXM85_001976"
     CDS             complement(1504..3342)
                     /locus_tag="VXM85_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1346490.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTLAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3360..>3576)
                     /locus_tag="VXM85_001977"
     CDS             complement(3360..>3576)
                     /locus_tag="VXM85_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1346491.1"
                     /translation="KLFAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQ
                     TEYNYLLTSSELAYLMAHIRRLTKNLD"
BASE COUNT         1194 a          725 c          477 g         1180 t
ORIGIN      
        1 tcttccaaac ttctttattc catatttaat gaaatgccac cttaaccgtg ataatagcta
       61 gtcatcaata aaaaactatt tgaataagga ttctccattt gattcaatca cttctttata
      121 ccaagtaaaa gacattttct tatatcgatt taatgtacca cttccatcat cgtttcgatc
      181 aacataaatg agaccgtacc ttttagaaag ttgtgcagtg gacatagaaa cacagtcaat
      241 acatccccaa gacgtatagc ccataatttc aacaccatcc tgtagagctt cagcaacttg
      301 caataaatgt tctttcatat actgaattct ataatcatct tggacggtta agttattaag
      361 ttcatctttt attagttgat ctttagcacc taatccattt tctactataa ataatgggat
      421 ttgataacgg tcataatatc tatttaaaat tatacgtagt ccaattggat caatttgcca
      481 tccccactct gaagactcta aataaggatt tactaaacca ccaataatat tcccttctcc
      541 tgaattatac tgtgttggaa gagcagattg agtcacactc atgtaatagc taaaggataa
      601 aaaatctacg gtataatttt ttaataactc tgcatcttca gctgcaaact ctatgttaat
      661 gtcattttcc ttaaaatatc tttttgcata attcggataa taacctctaa catgcacatc
      721 tgaaaataga taatttagat tctcatactc atgagtcgcc catacatctt ttggatttgg
      781 agtcattgga taagctggca tagctaatac catacatccc accttaaact ctgaattaat
      841 ctcacgagca atttttgtaa ccaaacttga ggcgactaat tcatgatgta tagcttgata
      901 taattcttgt ttcgaaagat tctccttagg tatatctatt cctccactag taaatggtaa
      961 ttccaaaaca gagtttactt cgttaaatgt aagccaatat ttaactttat ctttatacct
     1021 ttctaaaact gttcgagcaa atttttcata aaaatgaatc attctcctat caacccatcc
     1081 atgatatttt cttgctaaat ataatggagt ctcatagtgt gaaagagtta caagtggttc
     1141 tatcccgtga gcatgtagtt catcaaacaa ttcatcataa tatttcaacc cagcttcgtt
     1201 aggttcttcc tcatctcctt ttggaaaaat tctactccat gcaatagaag tacgaaaaac
     1261 attaaagccc atttcagaaa acaaggatat atcttcctta tatttatgat aaaaatcaat
     1321 acctatcaat tttaagttat cttctgtagg attttctgtt gcttctccta atccaccttt
     1381 gggtaacaca tcctgaactg ataagccctt accatcttca ttatatgctc cctctacttg
     1441 attagctgca acagctccac cccaaagaaa atcatctgga aaaatggtca taactttcct
     1501 ccattataat attaccagta attccttaga atgctcgatt gtctgattat taggtaatac
     1561 taatacatct agaaaatcat tggtattcgt tacaattact ggtgtaactg tttcgtagcc
     1621 tttagtcttg attaaattca agtccatttc aaaaatcaac tgatttttga aaactctgtc
     1681 tccttcttct acatgactaa taaaaccttg accttttagc tcaacagtat ctaatccaat
     1741 atgaattagt aactcaacac cctcatcact cttcaatcca attgcgtgct tagtcggaaa
     1801 aatatttgta attttcccat caaatggtgc ataaacctta ccttcacttg ggataatcgc
     1861 tactccgtct ccaattagtt tatctgaaaa tgttttatcc tggacatcgc ttaacggaat
     1921 gatttctcct gatataggag aaaatatcat ttttttattt gaaactccag cttcaacttc
     1981 taaattgcta gaactctctt cttcatcgat tccaaatata taagctaata caaaggtaat
     2041 aacaaccgaa atgaccgcca caattaaagc atttacaata tttgatggca catcagaata
     2101 aataaattga ggcaacgcta tcaaagatgg gacagcaaat agatatgctt taacactagt
     2161 aagacctgca aataatcccg ctaatccacc accaatcata gctgcataaa gcggtttttt
     2221 atattttaaa gtcacaccat ataatgcagg ttcggtaatc cctgcaagta aggctgagaa
     2281 acctgctgca aaagcaattt gttttgtatt attattttta ctctttaatg caacagccat
     2341 cgaagcagcc ccttgagcta agtttgaccc taacattgct ggaagaatta atacgtctgg
     2401 agtagcaata gatgccgcca aaaaaatagg tgcaaaagcc caatgcattc cagtcataac
     2461 aataaatggc ataatagcac caagaatagc taatgtaagc catccagcta caccatacat
     2521 ttgcccaact agatttgata atccttcacc aacaattact ccaataggtc cgactacaac
     2581 taaggcaata cagcttgata ctaataatac tagcgtaggt tgcaaaaaac tcttagtaat
     2641 agctagtgtt aatttagcaa ttattttttc aatatatttc atcaaccaaa ccataataag
     2701 aattggaacg actgatgaac cataactagc tggtgtcaca ggtgcaccaa ataaactaag
     2761 aggattccct gattgcacca tttgaacaaa atttggatgg agaagtacac ctgctacaga
     2821 catagctaat gtagatgtta cttttaattt ttgtgatgca gaataagcta ataacagcgg
     2881 taagaaataa tatggagcat ccccaaaaaa tgtcaaaaaa gcaatagtct gagaatctga
     2941 ttgcaatata ccaagcattg gtaaaatgat taccaagact ttcaacatac ctccccctaa
     3001 cattgctgga atgattggag tcatggaacc agcgatatac tcaatgattc tttctaaaat
     3061 attccctttg tgcccttgaa caactgaatc ggattcaaaa ttgccaagtt taacgaattc
     3121 tttataataa ttagctacat cattaccaag tataatttga tattgtccat tctttttcat
     3181 aatacctatt acacctggta tcttcttcac atcatcatca ttgactaaat tttcatcttt
     3241 taattctaat cttaaacgtg ttacacaatg ggtaactcta ttgacatttt tttcacctcc
     3301 aattacatcg aggatttttt gtaccgtatc tttataactc atggtattct cctattctat
     3361 taatctaaat tttttgttaa gcgacgaata tgagccatca aataagctaa ttcactagaa
     3421 gtcagcaaat aattgtactc cgtttgtata aacattgcta cctgttcacc acattcatat
     3481 tctctaggat atttattttt cattaatgct aacaagtctt catcatcatc gtcacaataa
     3541 gttgtatttt caaccaagcg atgggcaaat aatttt
//
LOCUS       DAVLRT010000119         3472 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLRT010000119 DAVLRT010000000
VERSION     DAVLRT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.119"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            4..1038
                     /locus_tag="VXM85_001978"
     CDS             4..1038
                     /locus_tag="VXM85_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1346492.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            1040..3283
                     /locus_tag="VXM85_001979"
     CDS             1040..3283
                     /locus_tag="VXM85_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEV1346493.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLDKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDRVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIAPDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VVLSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            3334..>3472
                     /locus_tag="VXM85_001980"
     CDS             3334..>3472
                     /locus_tag="VXM85_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1346494.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYKDFYEFQNRTYISRKENEEEYSN
                     LI"
BASE COUNT         1211 a          495 c          668 g         1098 t
ORIGIN      
        1 atcatgaaaa aatcagatgt tcttgattta ataaagtatc attatgaggg cagagagaca
       61 gaatttagaa atcaatccat agcaattgct cggaacttca ataaacatgg tgatacacaa
      121 atagcacaat atattatggg gttaatgtct caaagtgata gattcatgcc acaaatagaa
      181 aatcctagtg agtatttgac tcctgctaaa ctagatattg gtcctctccc attgcctctt
      241 tcaattatga atgatttaaa aggaataata aacgctgtca atcatcatat tggaataaat
      301 aaatttttat ttgtgggatc tccaggtact ggaaaaactg aaagtgtaaa gcaggtggct
      361 agacttattg gcaaggaatt acttgtagta gatttcagtc acttagtaga tagtaagcta
      421 gggcaaacag tcaaaaactt agcaactctt tttaatgaaa tcaataatct tcctttcaag
      481 caaaattata tcatcctatt tgatgaaatt gattccatag tgttggatag ggttaatcag
      541 aatgatttaa gagaaatggg acgagtgact tctgcctttt taaaggagtt ggataggctg
      601 tcaccagaaa ttgtattaat tgcaacaaca aatctttttg aaaatcttga taaagcagtt
      661 acgagaagat tcgatgccat aattgacttt gaccgttata ctgatgaaga taaagtagaa
      721 gtagctacta ttattttaaa tgagttatta aagcaattta agaatgtagc tagagattta
      781 aaattattta aaaaaattat caatagtgcc aatgtaatac cgaatcctgg tgatttgaga
      841 aactcaataa gaacgtcatt ggcatttagt gacccatcag atccgcatga ttaccaaaaa
      901 cgcctgctaa gaagtttgca taatggtaga aatttatcta tttctaaatt atcgaagcta
      961 ggttttactg ttagagaaat tgagattttg acaggtattt ctaagagtag tgtatcacga
     1021 gagttaagcg aggattaaaa tgaatacatt attgacatta cgaggtaaaa gttttacacc
     1081 aaagtcagca aaggggaggg gaccgataac gattcccgca gattctattg ttacgctaaa
     1141 ccatttaaat gaactttata catcccttga acgagtaaaa gaatattggt ctaataacac
     1201 aatcattgat ggagtactta ttagtgtgta ttataatcgt gtcctagcta agagcaatcg
     1261 tatcgatggt ttttttaatg tgggtggcgg aaatgcaatt ccaaatgaaa cgattgtggg
     1321 tgcaaaattt agtaatgata agagtaggca tattattaca cactacattt caaaggaagt
     1381 gttagataag acaattgagc atactaaaaa agttataaca atattcaaag attattttgc
     1441 tagtgctccc atgacgagta tggtgttctc cgactcttca aaatttaatc aaattgatta
     1501 ttctaagtat ggtctgtcga aatcaaaatt caaacaatac ttaagagacg cttgctttgt
     1561 caaaggattt ggtattgaac aggcagatat gtctactata cgtaattcta ttgtaacaat
     1621 ctacgatgta aaaactaatc tcttaacatt acttaaacaa cttgatattg atgtttcaga
     1681 agctaacatc atggaccaga caactgtttt acttgacgaa aaaaatattg agttactact
     1741 gctaaaagca ccttatctag tatcaatgat agtagaagat ttttctagcc tgtcaccaga
     1801 gaatttttat aatgatggga acacttttta taataatctc ccttttccaa ctaatgaacc
     1861 tattattggt gtcatcgata cgttgtttga tgatagagtt tatttcaatg agtgggtaga
     1921 gtatcatgat ttaatttcac cagatattag caaaggaagt caagactata agcacggtac
     1981 agcagtttcc tcggtaatag tagatggagc tagtttgaat ccaaaactag atgacggatg
     2041 tggaagattt agagtcagac attttgggat atctttacag agtggtttca attccttcac
     2101 aattatcaaa cagattagag aaattgtgtc tcaaaatcct gatattaagg tttggaacct
     2161 ttcgcttgga tcaaatgatg aaattaggga aaactttatt tctgtcgagg gttcgttgtt
     2221 agacgagatt caatttgata acgatgtaat ttttattatt gcaggcacta atgggattgc
     2281 ccctgatgga caacgaaaaa agattggagc accagcagat tcactcaatt caattattgt
     2341 aaattctgtt gattttagca atcaagtcgt ttcttattca cgcgaaggag ttgtcctgtc
     2401 tttctttatc aaacctgatg tttcatacta tggcgggggg aacggagact tcataaatgt
     2461 ttgtgaacca ctagggctgg ctcgagtagc tgggaccagt tttgcggctc catttattgc
     2521 tagaaaaatg gcttacctca ttcatattat gggattaagt cgtgaagaag caaaggcttt
     2581 attaattgat tcagcgatac catggaatga taaaaaaaca ttcgcagaca tttctttact
     2641 tggatatggg gttgtgccaa tcaaaataga agatattttg tctacacctg atgatgaaat
     2701 taaatttata gtctcagatg tttctagagc atatgaaact tataattatg atttcccagt
     2761 tccaatttct aaagaacact atccatatgt agcaaaggcc actatgtgct attttccaaa
     2821 gtgttctaga aatcaaggag ttgactatac taatacggaa atgcaactta gtttgggagt
     2881 tttagataag aaaggcatta aaacaattaa taacgacaat caatattctg aaaatgcacc
     2941 aggatatgtg agagaaaagt cggcacgaaa tatttttagg aaatgggata atgtcaaaca
     3001 tttaggagaa aaattttctt ctcgtaaaaa agctaagccg attctgaatc cttctaatcc
     3061 tcaatgggga atgagtgtaa aaacaattga acggttaaga aatgatgatg gttatggtat
     3121 ccgttttgga gtagttgtta ctctaaagga gttgaatggt gtcaatagaa tcgaagattt
     3181 tattcaacaa gccgaattac gcggttggct agttaataga ttgcaagtag aagctcaagt
     3241 agacctcttt aatagtttaa atgaagaaat tgaatttgaa tagtggatat tttatcctta
     3301 ttctagatct taggaacaca agagggcaaa gatatgaata ttacttatat agttggaaat
     3361 ggtttggatt tgcaatatgg tttgaaaaca agatataaag atttctatga atttcaaaat
     3421 aggacttaca taagtaggaa agagaacgag gaagaatatt caaatctcat tt
//
LOCUS       DAVLRT010000120         3450 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLRT010000120 DAVLRT010000000
VERSION     DAVLRT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.120"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            139..1374
                     /locus_tag="VXM85_001981"
     CDS             139..1374
                     /locus_tag="VXM85_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346495.1"
                     /translation="MKLYRCWYQTFVSDEASEETPLAERGEKEYIVKSSRMTALCYAE
                     SKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRYKLVSKLEFSP
                     NLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVITRTWANNVRLS
                     AITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHEGDTLNENYDK
                     VDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPIIEDDKIWFYY
                     MGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYDDEIYILADIP
                     ENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLGNSPVAFKFEI
                     ENAKLYGIEGSIQQHYLKY"
     gene            1443..3020
                     /locus_tag="VXM85_001982"
     CDS             1443..3020
                     /locus_tag="VXM85_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1346496.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPKINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            3080..>3450
                     /locus_tag="VXM85_001983"
     CDS             3080..>3450
                     /locus_tag="VXM85_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1346497.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNI"
BASE COUNT         1221 a          483 c          675 g         1071 t
ORIGIN      
        1 gctcaattaa aattaaataa gctaaaaaaa gatactgaaa acaatccact atttggtgaa
       61 gagtattttg ccgaaaatcc taaattatgg gaagttcgtt ttgataattc atatccaaac
      121 gttatctatg ataaagaatt gaaattatat agatgttggt atcaaacatt tgttagtgat
      181 gaagcaagcg aagagactcc tttagcagaa cgtggagaga aggaatacat tgtcaagtca
      241 tctagaatga cagcactttg ctatgctgaa agtaaagatg gagtaaaatg ggaaaaacca
      301 aatctcaatc tagtaaaatt taaaggttca aaagataata atattgttct gatgcatgca
      361 cacggtgcag gtatcatgct agatgaacag gagttggatc ctaagaaacg atataaatta
      421 gtttcaaaat tagaattttc tccgaatctt cattacatgg ttgttgtttt ttcagaagac
      481 ggtattcatt ttgacgaacc tattccatgg aaaaaatgga atcctgcagc agatagtcat
      541 aattttccat ttagagatcc aaaaactggt aaatacgctg ttattacaag aacctgggca
      601 aataacgtac gtctaagtgc aattactttc agtgatgatt ttattaattg gtcagaacct
      661 aaagaaatcc ttaggggaga tggatttgaa gatcaaattt attctatgcc agtaatttca
      721 tacgcggata tctatttagg ttttgcttca atgtaccacg aaggcgacac tttaaatgaa
      781 aactatgata aagtagactt agaattgaaa ttctctgcta atttggaaca atgggatagt
      841 gtatgtaagg gcgactatct cattgaaagg ggaggtggag tatacccgca gaatgctgat
      901 tttgatgcta gttgtatttt cacggctgct ccaataattg aagatgataa gatttggttc
      961 tactatatgg gtggaaatgg taatcacact ggcttccgag aaagttcatt agctagagga
     1021 tatattaaga aagaccaatt tgcttattat actaataaga gagaaggaat tccagctaaa
     1081 atatctacag ttagcttcca aacttacgat gatgaaattt atatcttagc tgatattcct
     1141 gaaaatggtt atattaaagt tgcggtaggt actaaatacg gtaatgctta ttcagatttt
     1201 ggtcaggaag atatctctct tgttcaagtt tccgatggta agtataagtt ggaatttaat
     1261 aaaaaatcta tcttagattt aggtaattct ccagttgcgt tcaaatttga aatcgagaat
     1321 gcaaaattgt atggtattga aggtagtatt cagcaacact atctcaaata ttaaccagaa
     1381 taattcgtta acaaataatc tctatattag tggagataga tatataaaat ggaggtctta
     1441 ttatggtaaa gcttaataag gtattaaaag ggagtgttgc attcctgagt gcaagtttat
     1501 tgctagcagc ttgtggcaat aacgctaatt caaagcccgc tgaaaaaaca gctgatggta
     1561 aaacgaaaat ttcgtttcta tctacatggc aaattaatga tgttcgtccg cctaaagatg
     1621 aaacacttgt tggtcaatgg ttagaagaac agctaggtat tgatattgat cttgtaacta
     1681 ttcctggcga gggagtaagt tctaaagtaa attctcttat tactagtaac cagttaccga
     1741 ctgttttgtt gacaacagga gataagagtg atatcgctgg tatcaacaaa cttggtaaac
     1801 agggtgcatt cttagatttg tcacaacaca tggacaaact accaaactat aagaagtacc
     1861 ttgaaaaaaa taatgcatta gctcaaatag aagatgatga aaaacatatt tatgctttca
     1921 caaaattctt tactgatgag aatatcatgt atactacacc aattctgaga aaagacttac
     1981 ttgttggttc agaatttgaa gatctttcaa aaattaaaac tgttgatgac tatacaaaag
     2041 ttttgaaata tttgactgaa aaacaaggtt ctccagcatt tattcaacgt aatggctatg
     2101 aaggcttcat gaagcgagtt acaccattgt ggaatttaag ccatcgtaca tactatgact
     2161 atgaatcaga ttcatataaa catccagttg aacaacctca attgaaacaa ttcgttgaat
     2221 ggttgaaaga attacgtaaa gataacgtac ttcaccctga ctgggcagtt atgaaagatg
     2281 aaacttggga aggtctattg gcttctaaca agggttcatt cactgttgac cgtatgaata
     2341 tcattggaga taataacttt gataaatcat ttgattggca gccacttgtt tatccaaaaa
     2401 ttaatggaaa acgttatttg caaccgaaaa caccgtacat ttctgattca ggttgggtaa
     2461 tcaatgccaa tgcaccaaaa gaagaaattg ataaagcgtt agaattcttt aatttcttat
     2521 acgctgatga aaatcaagaa aaacttgcta ttggatttga aggtaaaaca tttacacgtg
     2581 aaaaccctaa tactcaagca ggtattaaat ggttgattca aatttacggt gaaaatgctg
     2641 gaaatgataa agcagaactg ctgtttaaac atggtaacca agcatttact cgcgttcaaa
     2701 ctaaattaga tattgaggca cagccaggaa cattccctaa agttatgtat gatcaagtag
     2761 ataaaattaa gaaagattta ggtggtttta gaccagatac tccggttctt tcctttacaa
     2821 ctgaagaatt agatgtttta actaaatctg aagcaggatt aaatactttc tttgatgaaa
     2881 atattattaa attcattgaa ggacaaagag atatgagtga gtgggattca tttatcaatg
     2941 aagctaaatc taaagacttg gataaagtta caaaagtata taatgatgcc tacaaacgtg
     3001 tcaaagatag aatgaaatag actaaatggt agctctctgg tttttcagag agctactact
     3061 gcctgagggg gagtaagctt tgtatgatgt agtaattata ggtgcaggaa gttcgggcgc
     3121 attggctgca attgcttcgg ggcgtctagg gttaaaaact tgtctaatag agaagggaag
     3181 tagaattggt ggagttccta ttaatacact aatggggtca tttgctaatc tatattttga
     3241 tgattctggt agagtaaatg caagtaaaat tgttggggaa ttaatagaac gtattatcaa
     3301 aaaaggtggt actgtcttta actctatgga agaattgatg aatgtaaatg atttttatca
     3361 gattacaatt ccctatcaac cagaagttta tgaatcggtt ttaactgata tgttgattga
     3421 gtctaaagtt gatattttac taaatattga
//
LOCUS       DAVLRT010000121         3405 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLRT010000121 DAVLRT010000000
VERSION     DAVLRT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.121"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(189..800)
                     /gene="rpsD"
                     /locus_tag="VXM85_001984"
     CDS             complement(189..800)
                     /gene="rpsD"
                     /locus_tag="VXM85_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1346498.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(995..2047)
                     /locus_tag="VXM85_001985"
     CDS             complement(995..2047)
                     /locus_tag="VXM85_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1346499.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2044..2742)
                     /locus_tag="VXM85_001986"
     CDS             complement(2044..2742)
                     /locus_tag="VXM85_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1346500.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2909..>3405)
                     /locus_tag="VXM85_001987"
     CDS             complement(2909..>3405)
                     /locus_tag="VXM85_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV1346501.1"
                     /translation="ALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDN
                     IVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTN
                     TMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNK
                     KEEN"
BASE COUNT          942 a          765 c          651 g         1047 t
ORIGIN      
        1 ccataaaaga ttataccaca ttgtgtacta tttttggttc attttactat atcactttgg
       61 actatatcgg tctagttatg tctaaacgat tatagctgga tactaagaga aatcaaaaaa
      121 gcactaaggg agaacgtccc aaagtgctta gtttcaaggc tttacagcct atcttattta
      181 ataaaatatt acaacatttt gttgtagaat tcaacgacaa gtgcttcgtt gatttctggg
      241 ttgatttcgt cgcgttctgg caagcgagtc aatgaacctt ccaatttttc agcgtcgaat
      301 gatacgaatg ctggacgtcc aagagtagct tctactgctt caaggattgc tggaactttc
      361 aatgattttt cacgaactga gatcacttga cctggagtta cgcggtatga tgggatatca
      421 acgcgtttcc cgtcaacaag gatgtgaccg tggtttacaa attgacgagc ttgacgacga
      481 gtagtcgcga gaccaagacg gtaaacaacg ttatccaaac gacgttccaa aagaagcata
      541 aagttgaaac ctaggattcc gcctttgatt tttgtagctt gtacaaacaa gttacggaat
      601 tgtttttcac ctacaccgta agtgaaacga agtttttgtt tttcagccaa ttgcaaaccg
      661 tattctgaca atttagaacg gttgtttggt ccgtgttgtc ctggtacgta gttacgacgt
      721 gccaattctt tacctgtacc tgtaagtgaa aggccaaggc gacgagcttg tttccaagat
      781 ggtcctgtat aacgtgacat atgaatgtcc tcctgatata aataatattt cggtggaaat
      841 agtcacttag aaagccctga ttcgtgcaga tgcccttcgc ctaaacagcc aaggttactt
      901 atcataagac acctgttgac gagcttcatg ctttcctgct gatatttcac acaaaattca
      961 ttctaccatg aatagctgga tttgtaaagg ggttttaggc tttattttca ctaccagaga
     1021 ggttgagaac gagagtaaag gtacttccta gaccgtactg gctgctgact gtgatttccc
     1081 cacccaattg atgggccaat tcacgcgcaa tcgcaagtcc taatccatga ccacctgtct
     1141 tcatgttacg cgaagtttcg acacgataaa ggcgtttgaa aatattttcc aaatcctctg
     1201 gggcaatgcc ctgcccttca tcggtcacac tgattgaaag ctggcccttc tccagcttag
     1261 ccaccacttc cagcttggtt cctggagcag aatatttaaa agcgttatcg accagattca
     1321 ccaagatacg agaaagctta gcataatctc cctcaatccg ggcagactct gggattacct
     1381 gcaagtggac atctcttctc tcctgctcaa tcaaaaactg aaattcactc atgcactcaa
     1441 ttaagagctt gtccagaaaa atactgtctt tactggtagt ttccacctga tttctagctg
     1501 tgtttagggt caaaaaattc aactcctcaa ccagtttatt gagcctctcc gtctggcgtc
     1561 caatggttgc tagataatga gcttgctccg actccttgat aatcccatcc aaaatccctt
     1621 ctaccgtcgc ttggatcgaa gtgataggag tcttaatatc atgcgacaac tgagcaatca
     1681 tcaagccctt ttctcgttcg ctttcttcca aggaatcaaa gcttacctgc aaatcatggg
     1741 acatctcatt aaaagtttgc cctaattgct gaaattctac aggaccttga acctccaaat
     1801 ttgaaggaaa atccttggcc gctacccgct tggcatgctc cttgagtttg cccaacgacg
     1861 taaagactgg caataggaga aagagactaa tcccagcacc gacaaggctg gcaacgatgg
     1921 tcatcccaag caaaaagtaa atctcgcctt tcgcaatcag cattttttga acagcccaaa
     1981 aaacaaccaa aatggttaag agggttgaaa taatatatcc aaccaaaata taacttttta
     2041 gtttcatgtt tgtcctctcg gtttctctat cttatatccc aacccccaaa ctgtcttaat
     2101 agtgggcgtt tggtcactac tatattttgc cagctcctgt cgaagagcat ggatatgcac
     2161 attcaaggta ttggtgtcat ccacgtagtc ttctttccag atcttttcat agaggtctgt
     2221 cttggagaaa actcgctctg gattgctagc taaaatccac agcaattcaa atgatttaac
     2281 agttaaatcc agcatttctt ctcctatttg aacttcatga ctactatgat tcatttttag
     2341 attgccaagg gaaatcagct ctgtttcgcc cccacgatga aggcggcgca aaatattgtg
     2401 gacacgcaaa actagctcac gtgggctaaa aggcttagca ataaaatcat ctgctcccaa
     2461 gctcaggccg taaatcttgt cctgctcact ggtcttagca gtaataaata ggaaaggctg
     2521 ctctggtgat aagtattgaa cctcactgat taaatcataa ccatccatcc gaggcatcat
     2581 gacatctgtg ataatcaaat caatcggttt tttcttgaac agctctagcg cttccagtcc
     2641 atcatgggct accaagacct gataacctgc ctgaattaag tatctctgat gaatatctgt
     2701 gatttctacc tcgtcgtcaa cgagtaaaat tgtctttccc atctgtctct cctttgataa
     2761 aaacagtgct atactgcttt aagtataaca ctatttttat tgttttacga tgattttaat
     2821 ctgctattga aagaaagtcc tgattgctga ttgagttgtt aaactaatct tagtcagccc
     2881 actatccatc atttatttcc ttagctgatt agttttcttc ttttttgttt ttcaaaccta
     2941 gaccaccgag taaacctgca agcgcaagcc caaggaaacc aatacttgcc attgatgttt
     3001 gagtctcacc agtatttggt agcatagctt tatcctctga catcatcgta tcagacatct
     3061 tgttagcaga agcagccata ttttcacctg ccatcgtgtt ggtagaactt gtcatggtgt
     3121 cagcaggcat gctatctgta gtacctgtag catgattgtg attcatcgga gtcacgccag
     3181 aaccagagtt agaaggtgat aatgaaccat ttgctgtgtc tgatgtttct ttaacattta
     3241 tcttaatagt gactttttta gttgctacga tgttgtccaa gtctggttta ccgtctttgt
     3301 taccatagac attgactgta gcactgtaag tttgagtacc atttgctcgg aactggtcaa
     3361 tgagcgcttg tttttctttg ccagctacat ttccgtccaa ggcta
//
LOCUS       DAVLRT010000122         3287 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLRT010000122 DAVLRT010000000
VERSION     DAVLRT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.122"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..945
                     /locus_tag="VXM85_001988"
     CDS             <1..945
                     /locus_tag="VXM85_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1346502.1"
                     /translation="VGVNLIINKEINSLYLIGYLLAAMKIKDSLDASKEGLMEIFYLS
                     PKIERLKEIQNQDLQEGDDYSLKKFDIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTAL
                     VGASGCGKTTILKLISRLYDYDKGQILIDGKDIKEISTESLFDKVSIVFQDVVLFNQS
                     VMENIRIGKQDASDEEVKRAAKLANCTDFIEKMDKGFDTVIGENGAELSGGERQRLSI
                     ARAFLKDAPILILDEITASLDVNNEKKIQESLNNLVKDKTVVIISHRMKSIENADKIV
                     VLQNGRVESEGKHEELLQKSKIYKNLIEKTKMAEEFIY"
     gene            956..1585
                     /locus_tag="VXM85_001989"
     CDS             956..1585
                     /locus_tag="VXM85_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1346503.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYIVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            1486..2043
                     /locus_tag="VXM85_001990"
     CDS             1486..2043
                     /locus_tag="VXM85_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1346504.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            2031..>3287
                     /locus_tag="VXM85_001991"
     CDS             2031..>3287
                     /locus_tag="VXM85_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346505.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKNSPFVFFDEPSSGMDYSNMIK"
BASE COUNT         1223 a          404 c          637 g         1023 t
ORIGIN      
        1 gtcggcgtaa atctaattat taataaagag ataaattctc tctaccttat aggatattta
       61 ctagctgcta tgaagataaa agactcttta gatgcatcta aagagggctt gatggaaata
      121 ttttatttat cgcccaaaat agaaagatta aaagaaattc aaaatcaaga tttacaagaa
      181 ggcgatgact atagcttaaa aaaatttgat attgatctaa aagatgttga gtttgcctac
      241 aataaagacg caaaagtttt aaatggtgta agttttaaag ctaagcaggg agaggtcact
      301 gctttggtag gtgcaagtgg ctgcggtaaa acaactatct tgaaacttat atcaagactt
      361 tatgattatg acaagggaca aatcttaatc gatggcaaag atataaaaga aatatcaaca
      421 gaatctcttt ttgataaggt ctctatagtt ttccaagatg tggttctctt taatcaaagc
      481 gttatggaaa atattagaat cggaaagcaa gatgcaagtg acgaagaggt taaaagagca
      541 gcaaaacttg caaattgcac agattttata gaaaaaatgg ataaaggttt cgatacagtt
      601 attggtgaga acggagctga gctatcagga ggagaaagac aaagattatc aatagccaga
      661 gccttcttaa aagatgcgcc gatattgatc ttagatgaga taacagcaag ccttgatgtt
      721 aacaacgaga aaaagattca agagtcttta aataatttag ttaaagataa aactgttgta
      781 atcatttcac atagaatgaa atccatagaa aatgcagaca agatagtagt tcttcaaaac
      841 ggaagagtag aaagcgaagg taagcatgaa gagcttttac aaaaatcaaa aatttacaaa
      901 aatttaatag aaaagacaaa aatggcagaa gaatttattt attaggagga ctacaatgga
      961 taataaaaaa ttaaaagtaa aagatttagt aagtatcggt gtttttggcg taatttattt
     1021 tgccttcatg tttggagttg gtatgatggg cttgattcca atattgttct taatataccc
     1081 gacagtatta gccatagttg caggaactgt tgttatgtta tttatggcta aggttcaaaa
     1141 gccatgggca ctatttatat ttggtatgat atcaccactt gtgatgtttg cagctggtca
     1201 tacctacata gttgtggttt tatcacttat agtaatgata atagcagaat taattagaaa
     1261 gattggtaat tataattcat ttaaatacaa tatgctttct tatgcaatct tcagcacatg
     1321 gatatgtagc tctttaatgc aaatgctttt agcaaaagaa aaatatatgg agtggtcttt
     1381 gatgacgatg ggaaaagatt atgttgatgt attagaaaag ttaataactt atcctcacat
     1441 ggctttagta gccttaggtg ctttcttagg aggaattctt ggagcatata taggcaaggc
     1501 tctattgaaa aaacactttt caaatggatt atattgtgtg ggatacttta ctccttgcct
     1561 aattttatgg tgttatctga attaaaccct atagttaaga tgtttttgag tatacctatt
     1621 gttattagaa tgtttatttt accatttatg gcagcaagct ttatgataaa gacctcggat
     1681 gtaggcgcaa taatttcatc gatggataag cttaagattt caaagaatgt atccatacct
     1741 attgcggtta tgtttagatt cttcccatct tttaaggagg agaagaaaaa catcaaaatg
     1801 gctatgagag taagagggat aaattttaaa aacccagtca aatatcttga atatgtttct
     1861 gtgccactac tcattatatc atctaatata tcagatgaca ttgcaaaagc ggcagaaaca
     1921 aaggcaatag aaaatccaat tgccaagacc agatacattc gcgtaaagat acagctaatt
     1981 gattttgttt atgttttagc ggttgctgga cttattgtgg gaggattaat atggttgaaa
     2041 taaaaaattt aagtcttgat tatggtgaag agcatatatt agatgatata tcactatcca
     2101 tagccgaggg agagtgcgtg ctatttacag gaaaaagtgg aaatggtaag tcatctttaa
     2161 taaattcaat caatggacta gctgtaaggt atgacaacgc aaagacaaag ggcgaaataa
     2221 ttattgatgg taagaatata aaaaatttgg aactttatca aatctcaatg cttgtttcaa
     2281 ctgtttttca aaatcctaag acatattttt ttaatgtcaa tacgacatta gaattattat
     2341 tttatttgga aaatatcggt cttgcaagag aagagatgga caggcgtttg aaggatatgc
     2401 ttgagatatt cccgataaaa aatcttttaa acagaaatat atttaatcta tccggcggtg
     2461 aaaaacaaat tctttgcatt gcagcttctt atatagcagg tacaaagatt atagttatgg
     2521 atgagccttc atcgaattta gatattaaaa gcataagtgt tttggcaaag atgctaaaga
     2581 tattaaaaga gaaaggcata agcataattg ttgcagagca tagaatttat tatttgatgg
     2641 acatagttga ccgtgtattt ttaatagata aaggaaagct taaaaaaact tatactagaa
     2701 gtgaattttt aaagctagat aaaaatgaat taaatgcttt aagtttaaga gataaagaat
     2761 taagtaaatt aaaagttcct tatttaaaag aaggtggaga gtatcagata aaaaatctta
     2821 gctacaaatt tactgatgat gagtgtttaa gcttaaaaga tatttcgttc aagcttggga
     2881 aaatttatgg cataatagga tccaacggac gaggaaaatc aacgctttta agatgtttaa
     2941 taggtcttga gaaaaaatca aaagaagaaa tttattttaa gggagagaag ctatctaaaa
     3001 aagaaagact caaaaactct tcacttgtta tgcaagatgt aaatcatcaa ttattcacag
     3061 atgaagtatt caacgagctt agattaggag taaagaattt tgatgaagaa aaggcgaaaa
     3121 tcatcttaaa agatttaggc ctggacgaat ttattgaaag gcatccgatg agtttatcag
     3181 gagggcaaaa gcaaaggctt gcaatagcat ctgttatgtg caagaattct ccatttgtct
     3241 tttttgacga accttcaagt ggtatggatt attccaatat gataaaa
//
LOCUS       DAVLRT010000123         3280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLRT010000123 DAVLRT010000000
VERSION     DAVLRT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.123"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(168..422)
                     /locus_tag="VXM85_001992"
     CDS             complement(168..422)
                     /locus_tag="VXM85_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1346506.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(442..2283)
                     /gene="typA"
                     /locus_tag="VXM85_001993"
     CDS             complement(442..2283)
                     /gene="typA"
                     /locus_tag="VXM85_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1346507.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2445..3170
                     /locus_tag="VXM85_001994"
     CDS             2445..3170
                     /locus_tag="VXM85_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV1346508.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMNLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSLSQASITISEGKFHQIKKMFLSIGVKVTNLKRIQFGDFTLNPDLA
                     EGNYHPLNQKELQIIKNYLEMSR"
BASE COUNT          986 a          724 c          614 g          956 t
ORIGIN      
        1 aaaataatat gcaaccagta ctaaccaaat ataaaataga tgccattaac gaattttatt
       61 caagtttttc ccattcatac tatacaagca aaagagatgg tgttaactaa aaagcaattc
      121 aaactattat aaaattccta gcaaaaagag agccgaaact ctctttttta tcttctttta
      181 ctttttttga ctggcatgag tgtgatgtct ctcacactaa agtaagctag gatcaacatg
      241 gctattgcta ggaatatttc tgttggtaat tgaaaaattt tcagaaaaga tagaaccaat
      301 aaaatcaaga gtgccactaa aatacatacc atagcgacga tattgactgt ccctttaatg
      361 ctttctggtg tcgcaaatac atagagtagg agcagtaaaa ttcctaggac taaatagacc
      421 atctttctct ctttctagct cttattcagc tgattttttc ttcttgttag ctttctcacg
      481 ctctgctttg ttaaggattt gtttacgcaa acggatagac tcaggcgtta cttccatgta
      541 ctcatcgtcg ttcaagaact caagagactc ttcaagtgtc aagatacgag gcgtcttgat
      601 aacagctgtt tggtccttag tagctgaacg aacgttggtc atttgttttg ccttcgtgat
      661 gttaactgtc aagtcatttt cacgagagtt ttcgccgatg atcattcctt cataaacctc
      721 agtacctggg ttgacaaaga tcgtaccacg ttcttcgata gacatgattg agtaagttgt
      781 agccttacca gcatcgatag aaacaagggc accacggtga cgtccaccaa tttcccctgg
      841 aatcaatggc aagtactggt cgaaggtatg gttcatgata ccgtaaccac gagtcattga
      901 caagaactca gttgagtatc caatcaaacc acgcgctgga acaaggaaga ccaaacgagt
      961 ttgaccatta ccagttgaaa tcatatccaa catttcacct ttacgttcag aaaggctttg
     1021 gataacagac ccttggtatt cttctggagt gtcgatttgt acacgttcaa atggttcaca
     1081 tttaacaccg tcgatttctt ttacgataac ttctggacga gatacttgaa gttcatagcc
     1141 ctcacgacgc attgtttcga taaggattga caagtgcaat tctccacgtc ctgaaacagt
     1201 ccatttatct ggtgaatcag ttgggtcaac acgaagggaa acgtctgttt gcaattctgc
     1261 ctgcaagcgt tcttccacct tacgagaagt tacccattta ccttctttac cagcaaatgg
     1321 tgagttgttg accaaaaaag tcatttgaag agttggctca tcgatgtgta ggattggaag
     1381 agcttctact gcatctgtcg gagtgatggt ttcaccgaca aagatgtctt ccatacctga
     1441 aacggcaatc aagtcacccg ctttggcttc ttggatttca cgacgttcca aaccaaagaa
     1501 accgaagagt tttgtaacac ggaagttttt agttgtaccg tcaagtttag aaagggtaac
     1561 ctggtcccca accttaactg taccacggaa gacacgaccg ataccgatac gtccaacaaa
     1621 gtcattgtag tccaaaagtg acacttgaaa ctgcaaaggc tcatctgagt tatctactgg
     1681 agctgggata tggtcgataa tcgtgtcaaa gattggtgcc atagtcgctt cttggtcagc
     1741 tggatcatct gacaatgaag aagttccgtt gatcgctgaa gcataaacca ctgggaaatc
     1801 aagctggtcg tcatctgcac caagctcgat gaaaagttcc aagacttcat ccactacttc
     1861 tgctggacga gctgatggct tatcgatttt gttaacaacc acgattggga caaggtcttg
     1921 ttccaaggct tttttcaata cgaaacgagt ttgtggcatg gttccttcat aggcatctac
     1981 gaccaagaca acaccgtcaa ccattttcat gatacgctca acttctccac caaagtccgc
     2041 gtgtcctggt gtgtccataa tgttgatacg agttccgttg taagcaacgg cagtgttttt
     2101 agcaaggatg gtaatcccac gctctttttc gatatcgttt gagtccatag cacgctctgc
     2161 caattcagta cgtgcatcaa gcgtttctga ttgtttcaat aattcgtcaa ccagggttgt
     2221 tttaccgtgg tcaacgtggg cgataatcgc aatgttacgg atatcttctc ttaattttgt
     2281 catgatttcc tctataatat tcaaaattta ttttctaact gaacgattat accataattt
     2341 caaataaata acataactca agcaagtgta aatgttttca ctccgctttt cttttcacgt
     2401 caagcctttt caaagcaagc gacttatgat aagataggca cagtatgcgt ttagataatt
     2461 tattagccca agaaaaagtt agccgaaagg ccatgaagca agcactcctc agaggggaaa
     2521 ttctagtcga tggttgccca gcccgctccc tagctcaaaa tatcgataca ggactacaag
     2581 aactcctttt tcaggatcga atcattcaag gctatgaaca cacctatctt atgcttcata
     2641 aacctgctgg tgtcgttaca gccaacaaag acaaggaact tccgaccgtc atgaacttgc
     2701 ttccatctga catccagtct gacaagctct atgccgtcgg ccgactggac cgagatacaa
     2761 caggactcct tctcttgacc gataacggtc ccttgggctt ccaactcctc catccccaat
     2821 atcatgtcga taagacttat caagttgagg ttaatggact tctaacacct gaccatatcc
     2881 aaacctttca aaaaggaatt gtctttttag atggcactat ctgtaaacct gcaagactag
     2941 agattctatc agcaagtcct tccctcagtc aagcctctat caccatttca gaaggaaaat
     3001 ttcatcaaat caagaaaatg ttcctctcga ttggtgttaa ggtgactaac ctcaagagaa
     3061 tccaatttgg ggacttcaca ttgaacccag atttagcaga aggtaactac caccctttga
     3121 accaaaaaga gttacaaatc attaaaaact atttagagat gagtcgataa aacaaaaaaa
     3181 gctttaaaac taaagctttt ttcttttatt taccgaaaaa ttaaggcgat tgctacaatc
     3241 cagttaacta cagaaatcac aattcctaag atattaagaa
//
LOCUS       DAVLRT010000124         3276 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLRT010000124 DAVLRT010000000
VERSION     DAVLRT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.124"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..177
                     /locus_tag="VXM85_001995"
     CDS             <1..177
                     /locus_tag="VXM85_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1346509.1"
                     /translation="ENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVT
                     LITGKNGTGKSTLL"
     gene            complement(543..1853)
                     /locus_tag="VXM85_001996"
     CDS             complement(543..1853)
                     /locus_tag="VXM85_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1346510.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGKTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2090..2962
                     /locus_tag="VXM85_001997"
     CDS             2090..2962
                     /locus_tag="VXM85_001997"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1346511.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFKRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALDDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3093..>3276
                     /locus_tag="VXM85_001998"
     CDS             3093..>3276
                     /locus_tag="VXM85_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1346512.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVKGTKPRKVD"
BASE COUNT         1029 a          547 c          736 g          964 t
ORIGIN      
        1 gaaaacagaa ctgtttcttt tgctattagt tcaaaaggag aaaaaatgaa agtagaaaat
       61 atttcgtata gggtggatca tcgtatattg tttgataata tttcttttga tacttcgagt
      121 tcaggcgtga cattaattac tggtaaaaat ggtacaggaa agtcaacttt actatagtag
      181 attgaaacta gaatagtaca catctacttc taaaatattg ttagaaatcg atttgactat
      241 cctgatctat ttgtcctgtt cttatttcat ttcactacat ctcaaattga gtatgacgaa
      301 gtgcgctccc atgtcctggg aacgcacttt cttcatattt ttcatattct tgaatccatc
      361 gataaagact attgggatga atttttaaag ttgaactaat catttttaca ggatgaggtt
      421 tacagcagag agtttgaagg ctttatcaaa ggtttttctt ggcataatga cttttcctcg
      481 tttccactta attttgtgtc tactttatta taccaagtcc acccttaagt tagataataa
      541 atctaactta aggaagctag aaggatgaga atccaggtgg tcaagagtcc caaacttaag
      601 ctgatgggga cacccagaat aatttgcttt ttgaaggcaa ggccacgttc ctctatattg
      661 ggaagtgaga gttgaatgag agaaccagct gatgaaaagg gtgagatatt agtagataga
      721 gcgccaataa cggtggctgt tgtgagtaag tgaatatcaa tctggggatt ttgagcactg
      781 atgatagcaa tgatgggaaa gagggctgga gctacaacgg atagggtgga actaaagagt
      841 gacatcactc cggctatcac acaaaagaac agaggtaacc agaaatgagg aatggatgtt
      901 gtcatgaggt gccctatcag tgtgactaaa cctgacttga ccgctagaga cattagtaag
      961 ctcatgccgc agagcatgat aattgtagcc cagggaacct tagctaaaat ggcttcttgc
     1021 ttccctaatt tgagccttaa ggcgaggcag accatgagta ttgagacaaa gccaatatca
     1081 aatgtttttt gataagtagc tatccaggcg atgtttggga aaatgagatg caacaaggga
     1141 aaaagccaaa ccaaaaccat gctgctgatc atgagcaagg tggtttgtct ttgaaccttg
     1201 ctgaggagtg gtggttggtc aatagtcaag gatgagtttg tttttccctt actatagtga
     1261 ctgtaacagg ataataaaag caagacgatg agtgggtaga taatgctgac gataaagata
     1321 tgattgccaa gtgaaaaagc ttgctcttcc catcccattt gcttaaacag gccttgaaag
     1381 acaatgcctg agctactggt tatcaaatta gcccctcctg aagctcccca attgacggct
     1441 tgagctccaa tcaaagggtg tttgtccgct ttttgacaga gggtaatcgc tagaggacag
     1501 caaacggcca tagtagtgaa aaatccagca cctaaagcag acaaaagggt tgccatcagg
     1561 tataaaatca tgtagagggc gttagggtag gtgcgtgtgc ggtagagaat gtgttgagcc
     1621 aaaacatcaa gagtaccgtt agttattgca aagttataaa agagagagac gctaaaaatg
     1681 gtaaaaaaga gtgaggttgg ccaaaaatga agaagttctt tggggcttaa tcccatgaga
     1741 gtggttgcga tgaggtaaga aaaagcaata gccagcaggc caatattgat tttggtgcgg
     1801 taaccaattc caatggctag agcaatggcg ctaatcatta ttaaatgaat cattgaattg
     1861 tcctttcagt tagaatatgg aaataggttg gcaattttgg cgctcaaaag accacctgtt
     1921 tcctattggt acttgactat tatacacaaa aaaaatacaa ttgtcttgac aattgtattg
     1981 acacttgtta gaattaaaaa tgaggtattc aggcttgtga tttcctatgg aagttaatag
     2041 tgattgcctc taatgcttac aagtgatatt aaaaatagag gacttagtga tgtcaatcat
     2101 ttcaactgat ttaacccctt ttcaaataga tgatacattg aaagcagcct tgcgagaaga
     2161 tgttcattcc gaagattaca gtaccaatgc catttttgat catcatggcc aagccaaggt
     2221 gtcgcttttt gccaaggaag ctggtgtttt agcggggcta accgttttta aaagggtttt
     2281 taccctattt gatgccgagg tgaccttcca gaatcctcat caatttaagg atggggatcg
     2341 tttgactagt ggcgatttgg ttttagaaat cataggctcg gtgagaagtc tcttaacatg
     2401 tgaacgcgtt gccttgaatt ttttacaaca tttatcaggg attgcttcga tgacagctgc
     2461 ttatgtagaa gccttagacg atgattgcat taaggtattt gatactcgaa aaactactcc
     2521 taatttacgt ctttttgaga aatatgccgt gagagttggc ggtggctata atcatcgctt
     2581 taatttatca gatgctatca tgctaaaaga caatcacatt gcggcagtag gtagtgttca
     2641 aaaggcaatt gctcaagcgc gtgcctatgc tccttttgtg aaaatggtcg aggtggaagt
     2701 ggaaagcctt gctgctgccg aagaagctgc ggcggcgggt gctgatatta tcatgttgga
     2761 taatatgtca ttggaacaga ttgaacaggc cattacccta attgcaggac gttctcggat
     2821 tgaatgttct ggaaatattg atatgaccac tattagccgt tttcgtggtt tagcgattga
     2881 ttacgtctcc agtggtagtt taacccatag tgctaagagt cttgattttt ccatgaaggg
     2941 gttaacctac cttgatgtct aagttgtaaa ataaactaac tttttaaagg atgtctttcc
     3001 tctagaacga gttttatgtc agatagttta aacgcctctt caaatatagt aaaatgaacc
     3061 aaaaatagta cacaatgtgg tataatcttc ttatggcata ttcaatagat tttcgtaaaa
     3121 aagttctctc ttattgtgag cgaacaggta gtataacaga agcatcacac gttttccaaa
     3181 tctcacgtaa taccatttat ggctggttaa agctaaaaga gaaaacagga gagctaaacc
     3241 accaagtaaa aggaacaaaa ccaagaaaag ttgata
//
LOCUS       DAVLRT010000125         3250 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLRT010000125 DAVLRT010000000
VERSION     DAVLRT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.125"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            106..270
                     /locus_tag="VXM85_001999"
     CDS             106..270
                     /locus_tag="VXM85_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1346513.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            354..920
                     /gene="amaP"
                     /locus_tag="VXM85_002000"
     CDS             354..920
                     /gene="amaP"
                     /locus_tag="VXM85_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1346514.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            932..1102
                     /locus_tag="VXM85_002001"
     CDS             932..1102
                     /locus_tag="VXM85_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1346515.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1133..1741
                     /locus_tag="VXM85_002002"
     CDS             1133..1741
                     /locus_tag="VXM85_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1346516.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1772..1975
                     /locus_tag="VXM85_002003"
     CDS             1772..1975
                     /locus_tag="VXM85_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1346517.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2085..2357)
                     /locus_tag="VXM85_002004"
                     /pseudo
     CDS             complement(2085..2357)
                     /locus_tag="VXM85_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2953
                     /locus_tag="VXM85_002005"
     CDS             2666..2953
                     /locus_tag="VXM85_002005"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1346518.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
BASE COUNT         1115 a          507 c          617 g         1011 t
ORIGIN      
        1 tttttgatat taaaataaaa aaggagggta ggcagtgttg tgatcaatta ttgctggagg
       61 tcttattggt ctcttggcag gtaaaatcac taaaaaagta gttctatggg aatcatcgca
      121 aatgtattcg ctggtttagt cggggcatat gcaggacaat ctcttttagg tagttggggt
      181 ccatcaatcg ctggaatggc tttgctccca tctattgtag gtgcagcgat tgtgattact
      241 gtagtgtcat tctttacagg tagaaagtaa acttttcgcc agtaaagtta gcaaactatt
      301 tttaaatcaa tgacgggaaa aatagtttaa atgttaaatc gaaaggattg tatatgtcaa
      361 aagcaaagaa aatatgtttc attattttct gtattttaat cttgacaatt ttccttcctg
      421 ttttgataga ttatcatcaa gttagtgatc taggtattca tctacttagc tggagacaga
      481 actccgtagt tgaattctat cttgctagat atgtcttttg ggggacagtg gttctatcaa
      541 ctttagtttt attatccatt ttagttgtga tgttttatcc taaacgttac ttggaaatcc
      601 aacttgaaac taaaaacgat acattaaaat taaagaattc ggcaatcgaa ggttttgtta
      661 gaagtttggt gagtgatcat agattgatca agaacccaac tgttcatgta aatttacgaa
      721 aaaataaatg tttcgttcat gtagaaggta aaattcttcc ttcagacaac atcgctgaca
      781 gatgccaaat aattcaaaat gaaataacta atggattgaa gcagtttttt ggtattgagc
      841 gtcaagtaaa acttgaagtt gcagtaaaaa attaccaacc aaaacctcaa aacaaaaaga
      901 ctgttagtcg tgtgaagtaa ggaagtaaaa aatggaatgg cttaaacaat atcgatatcc
      961 aattatcgct ggtctcatag gcgtatttct ggcttgtttg attgtctcct ttggcttctt
     1021 caaaacaata tttgtattga ttttaggagc actgggagtt gcagctggat tatatatcga
     1081 aaaaaactat atagataaat aaaaattact aatttaatta aaggagtttc atatgtcaaa
     1141 cgaaaaaaac acaaatacta acgtagaaaa gaaagatgct actgttgtag ctcacgaaat
     1201 caaaggggaa cttacttacg aagataaagt tatccaaaaa atcattggtc tttcactaga
     1261 aaacgtttca ggtcttttgg gaatcgatgg tggtttcttc tcaaatctta aagaaaaaat
     1321 cgttaacagc gatgacgtaa caagtggtgt taacgtagaa gttggtaaaa cacaagttgc
     1381 agttgactta aacgttattg ttgagtacca aaaaaatgtt ccagctttat attcagaaat
     1441 cagagaaatc gtatcttcag aagttgctaa aatgactgac ttggaaattg ttgaaatcaa
     1501 cgtaaacgtt gtcgacatca aaactaaaga acagcatgaa gcagactcag taagccttca
     1561 agatcgcgta tctgacgttg ctgaatcaac aggagaattc acttcagaac aattcgaaaa
     1621 agctaaatct ggtcttggat ctggtttctc aactgttcaa gaaaaagtta gcgaaggtgt
     1681 agaagctgtt aaaggtgcag caaatggtgt agtatctcac gaaaacactc gtgtaaacta
     1741 agataaaata aatataacag gagaaattat catgtcagta gaagaaaaat taaatcaagc
     1801 taaaggttct attaaagaag gtgttgggaa agccatcggt gatgaaaaaa tggaaaaaga
     1861 aggagcagct gaaaaagttg tttctaaagt aaaagaagtt gccgaagacg ctaaagacgc
     1921 tgtagaaggt gctgtagaag gtgttaaaaa catgttgagt ggcgacgata aataaggtta
     1981 aaagttactt tatctttttg gtaacattag tcaaaagagt ctgagtcaag atgattctca
     2041 gaaaacaaaa agctagagat tcccaattgc ggaactctag ctttttaatt ttgcctcttt
     2101 ctcttattat atttcagcag gttgttggcc atgagtatga atcccatgtc aattctcact
     2161 tgacgcttac ctctcagatg acatctctta taacccaaac aaacctttat ctgcccaaag
     2221 acagatttca tatcaatctt acgtttagcg aaaatttgtc tacccttgga agataaaagt
     2281 gcctgatatt ctttagtttt taaacactgg taacgttcat tcatatacag tcccttttga
     2341 ggggctgatt caggttcata atcgcagtca acattgattt caaggctgtt tgctttctat
     2401 ctcccctttt tcataatgta tagtaaaatg aaataataac aggacgaatc gatcgggaca
     2461 gtcaaatcga tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaatcta
     2521 ctatattttc gtacaggtgc ttcaatagtt tctatagtaa aatgaaccaa aaatagtaca
     2581 cgttgcggta taatgttctt gtggcatatt cattagattt tcgtaaaaag ttctctccta
     2641 tttgtgagcg aacaggtata gtcaattgat tgtcagtttg aagaccaaga gtcgtaaggc
     2701 aaaggatatg gcagaatcca tccaagggtg gctggcacaa tttctagtga acctctttaa
     2761 atctattacg tttgactgtg gaaaagagtt ttcaaaatgg aaagatattt ccaatcatca
     2821 cgatagtgag agcttttttg cgaatctagg atgtccaaga cagcggtgtc tcaacgaaca
     2881 ttcaaataga cttttaaggt gtcatgactt gccaaagcag acagatttca acgaagtcag
     2941 tcaagaattt taggctgggt aaaaactcaa tttcaggaga aaatgaaata aatattccca
     3001 caataaaacg catagtatca aagtttttcc aacacctgat attatacatt ttttaatttt
     3061 aaagactttt tcccagcctc aagaaatcac atcccaccta aatcctttgg ataccaaata
     3121 cctcatcagg tattttctga gttggtaaga ctgtctagct taatttgaca aatggagggc
     3181 gttaaatcca gtttttacag gaagtctatt taaatatttt cacactgacc ttacaaaata
     3241 tgaaataaat
//
LOCUS       DAVLRT010000126         3193 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLRT010000126 DAVLRT010000000
VERSION     DAVLRT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.126"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..213
                     /locus_tag="VXM85_002006"
     CDS             <1..213
                     /locus_tag="VXM85_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346519.1"
                     /translation="FLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLF
                     SVLLLFSNFSVPFIKAKEYELFKNSK"
     gene            1346..1585
                     /locus_tag="VXM85_002007"
     CDS             1346..1585
                     /locus_tag="VXM85_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346520.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1570..1848
                     /locus_tag="VXM85_002008"
     CDS             1570..1848
                     /locus_tag="VXM85_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346521.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            2137..>3193
                     /locus_tag="VXM85_002009"
     CDS             2137..>3193
                     /locus_tag="VXM85_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV1346522.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVAKQSQAER
                     DYDAAMKKSEAAKKEYEEAKKDLEEAKAAQKKYEDDQKKTEEKAKLVQKADEERQKAN
                     VAVQKAYLKLREAQEQLNKSPNNKKNAAQQKLKDALAHIDEVTLKQKEAEANFNKEQA
                     KVVPEADKLAETKKKAEQAEKKEPELAKKVAEAKAKAEEAEKKAVEAKQKVDAEKYAL
                     EAKIAELEYEVQGLEKELKEIDESDSEDYIKEGLRAPLQSKLDAKKAKLSKLEELSDK
                     IDELDAEIAKLEKDVEDFKNSDGEQAEQYLVAAKKDLDAKKAELENTEADLKKAVDEP
                     ETPAPAPKPAPAPAPKPA"
BASE COUNT         1166 a          440 c          627 g          960 t
ORIGIN      
        1 tttttattca tcagattatt tgacacaagt actttaatga agaggagaat attttttctt
       61 ttcgcattgg atgttatagc ttacttattt ttaggatatc ttttagtgat tcaaaaagca
      121 ggctatttgt tttcggtttt acttcttttt tctaattttt cagttccatt tatcaaagca
      181 aaggaatacg aattatttaa aaatagcaag taatagaaac ataggttaaa tcttagaacg
      241 gcttaaaatc agatatgata aagggaaata gtcttatcta ggtaactaag tgctacaaac
      301 acagtcgagg atcacttgtt aggattacat tttaatcatg cttaataaga atggagaatt
      361 cctatgggga ttgtattact ctgaagtttc gaatacctta gttctcgtga ttttgagctg
      421 ttctagcttt agaatccttc agaatgtaaa attttaagca gtcaaagact tagaatagcc
      481 tggaaacatt tattttatag caattctagg cttagaattg ctcatatata tgtatttgag
      541 ctgctcgata gttaggacag ctctattctg agaaatctga gctatcaatg cttcagaata
      601 gtatttcgca aatggttttt attgaagagg tgattgtgtt atgatagtct aaaataaaac
      661 tgatttaatg gagtaaatgg agaatcgtca catgaaacat ttttttgcag gaattggtga
      721 gataaaactt gttggctact tattgtatat atgtgtagga ctagcccttc tttttcatat
      781 atatgtcata ggttcggaac tgagttttgt gaaaatagtg ttatctattt tgggagttat
      841 atttgtaagt atgttaacta ttgctcgaat ttatcggact tttctataaa gaatagtttg
      901 gacttgaact gtatctaaaa acagaggttg gtcgattatc agcctctttc ttgtcgttga
      961 aaagagtgag atatattgac ttttgaaaaa aatgtcataa ttgagttggg ataaacctaa
     1021 aagtaatata gtagattgaa tctggaatag tccgttatga ctgttaaaat atttctagaa
     1081 attaatttga ctttcctaat cgatttgttc acatcttgtt tcaatctact atactttctc
     1141 gttaaaccgt tcggcaattc atggtgacat ggacacatag tagtaagcac actatgtggc
     1201 ttggcagagc taaaaactgt tcccttgcga taagcctaat aagcacaaca tagggagtta
     1261 gagaagccga ctctaatcaa ccactttggg cagtagtgag aactgccccg tgctttttta
     1321 ttttgagaaa atatggagtt tgtcgttgaa attacttgat tgtattttgg actatcaaga
     1381 aagattcaat ggaaaaacat gtcaagtatc aacgaattat aagtatttag agattttcaa
     1441 agtgaatttt tgcttgactg atttacatca tttatttgac ttatacaaaa tcacacgaga
     1501 ttatgctagt caaacaaaac ctgctattca agctggtgtt tttattttag aagattttag
     1561 aaatatccta tgtacaatga tgtaatcgaa aggatatctt tatatgagtt tatcggtgat
     1621 attttctatt ctaaaataat aagttgttgc atcgtagcta gggatttatc taaaaatact
     1681 atgaaattgg acgtcatatt ttttgaggat aaaaataaaa gatccgcagt tttaggttta
     1741 cgaagagaca aaagcggagt atttaaatca gttactctac attttacaag cgctaagaaa
     1801 tatgctaaag ttcgtaaaac agatgtgaaa gaaatgaaat ggctataaag attgtccgca
     1861 ggcttaagct tgcgtttttt gttgtttaac gaaattaaaa aacaagctct ctcatcggaa
     1921 atgtttttat aggaatgaag gaagatgatg ctatgtttaa aaagagaatc gaaaagggca
     1981 aaagtagtat ctttatctat tttttggagt agaagcttat gatatagaaa tttgtaataa
     2041 aaatgtaata taaaacattt gacaaatatt tacgggggga gtatacttaa tataagtata
     2101 gtctaaaaat gactatcaga aaagaggtaa atttagatga ataagaaaaa aatgatttta
     2161 acaagtctag ccagcgtcgc tatcttaggg gctggttttg ttgcgtctca gcctactgtt
     2221 gtaagagcag aagaagctcc cgtagctaag cagtctcaag ctgagagaga ctatgatgca
     2281 gcaatgaaaa aatctgaagc tgctaagaaa gaatacgaag aagctaagaa agatttagaa
     2341 gaagcaaaag ctgctcagaa aaaatatgaa gacgatcaaa agaaaactga agagaaagca
     2401 aagcttgttc aaaaagcgga tgaagagcgt caaaaagcga atgtggcagt tcaaaaggcg
     2461 tatttaaaac tccgagaagc acaagaacaa ttgaacaaaa gtccaaacaa taagaaaaat
     2521 gccgctcaac agaaattaaa agatgcatta gctcatattg atgaggtaac attaaaacaa
     2581 aaagaggcgg aagcgaattt caataaggaa caagcaaaag tagttcctga agcagataag
     2641 ttagctgaga ctaagaaaaa agcagaacag gctgaaaaaa aagaaccaga acttgctaaa
     2701 aaagtagcag aagctaaagc aaaagcagaa gaagctgaga aaaaagctgt tgaagccaaa
     2761 caaaaagtgg atgctgaaaa atatgctctt gaagctaaaa tcgctgagtt ggaatatgaa
     2821 gttcagggac tagaaaaaga gctcaaagag attgatgagt ctgactcaga agattatatt
     2881 aaagaaggtc tccgtgctcc tcttcaatct aaattggatg ccaaaaaagc taaactatca
     2941 aaacttgaag agttgagtga taagattgat gagttagacg ctgaaattgc aaaacttgaa
     3001 aaagatgtag aagatttcaa aaactcagac ggtgagcaag ctgaacaata tcttgttgca
     3061 gctaaaaaag acttagatgc taaaaaagct gaattagaaa atactgaagc tgaccttaag
     3121 aaagcagttg atgagccaga aactccagct ccggctccta aaccagcccc agctccagct
     3181 cctaaaccag ccc
//
LOCUS       DAVLRT010000127         3035 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLRT010000127 DAVLRT010000000
VERSION     DAVLRT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.127"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            247..1533
                     /locus_tag="VXM85_002010"
     CDS             247..1533
                     /locus_tag="VXM85_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1346523.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     TLIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            1548..1859
                     /locus_tag="VXM85_002011"
     CDS             1548..1859
                     /locus_tag="VXM85_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346524.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2094..2822
                     /locus_tag="VXM85_002012"
                     /pseudo
     CDS             2094..2822
                     /locus_tag="VXM85_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT          943 a          531 c          662 g          899 t
ORIGIN      
        1 gtagtcatct aaggactaat ttatgtattt ttacgagtca gtatctttgg atactggttt
       61 ttacttttct agactttttg actacttgtt aaaactggga taattttcga ctgtttaaca
      121 gttattatgc aaagtctaaa agattagaat tgtcaaaaca atccgtctag gcttgatttt
      181 atcctttatt tactataaaa tgagaaggaa aaatgtcaaa cttttatatt gcaaatagga
      241 gaaattatga caaaaacatt aaaacgtcct gaggttttat cacctgcagg gactttagag
      301 aagctaaagg tagctgttca gtatggagca gatgctgtct ttatcggtgg tcaggcctat
      361 ggtcttcgta gccgtgcggg aaactttact ttcgaacaga tggaagaagg cgtgcagttt
      421 gcggccaagt atggtgccaa ggtctatgta gcggctaata tggttatgca cgaaggaaat
      481 gaagctggtg ctggtgagtg gttccgtaaa ctgcgtgata tcgggattgc agcagttatc
      541 gtatctgacc caaccttgat tatgattgca gcgactgaag caccaggcct tgaaatccac
      601 ctttctaccc aagccagtgc cactaactat gaaacccttg agttctggaa agagctaggc
      661 ttgactcgtg tcgttttagc gcgtgaggtt tcaatggaag aattagctga aatccgcaaa
      721 cgtacagatg ttgaaattga agcctttgtc catggagcta tgtgtatttc atactctgga
      781 cgttgtactc tttcaaacca catgagtatg cgtgatgcca accgtggtgg atgttctcag
      841 tcatgccgtt ggaaatacga cctttacgac atgccatttg ggaaagaacg taagagtttg
      901 cagggtgaga ttccagaaga attttcaatg tcagccgttg acatgtctat gattgaccac
      961 attccagata tgattgaaaa tggtgtggac agtctaaaaa tcgaaggacg tatgaagtct
     1021 attcactacg tatcaacagt aaccaactgc tacaaggcgg ctgtggatgc ctatcttgaa
     1081 agtcctgaaa agtttgaagc tatcaaacaa gacttggtgg acgagatgtg gaaggttgcc
     1141 caacgtgaac tggctacagg attttactat ggtacaccat ctgaaaatga gcagttgttt
     1201 ggtgctcgcc gtaaaattcc tgagtacaag tttgtcgctg aagtggtttc ttatgatgat
     1261 gcggcacaaa cagcaacaat tcgtcaacga aatgtcatta acgaagggga ccaagttgag
     1321 ttttatggtc caggtttccg tcattttgaa acctatattg aagatttgca tgatgccaaa
     1381 ggcaataaaa tcgaccgcgc tccaaatcca atggaactat tgactattaa ggtgcctcaa
     1441 cccgttcaat caggagacat ggttcgtgca ttaaaagaag gactcatcaa tctttataag
     1501 gaagatggaa ccagcgtcac agttcgagct taagaaagga aaaggaaatg atagaggcac
     1561 agggtttctt agtggataag caaacaagat gcattcatta ccatagcaag ctggatatta
     1621 ttgctttaca atgctatgat tgtaaaaagt attatgcttg ttatcggtgt catgattcat
     1681 tagaaaatca cccttttgag ccgtatccct tatctttgat acaggataag cctattttat
     1741 gtggtgtttg tctaaaacta ctaacatata agcaatataa agaaagctta agttgcccct
     1801 tttgtttttc tcgctttaat ccaggttgcc aaaatcataa ggaacgctat tttaaatagc
     1861 aaatcatcta gttttgaagt aggagaaaac tcaattccaa gagaaaatga agtaaatctt
     1921 cccacaataa aacgcataat atcaagattg ttcaatacct gatactatgc gtttttaaga
     1981 ttttaaagac ttttttcctt tatctggtat tttgactact tgttaaaact gggttaattt
     2041 tcaactgttt aacagttatt atgcaaagtc taaaagatta gaattgcgtc tagagtatgc
     2101 gtgatgccaa ccgtggtgga tgttctcagt catgccgttg gaaatacgac ctttacgata
     2161 tggcatttgg gaaagaacgt aagagtttgc agggtgagat tccagaagaa ttttcaatgt
     2221 cagccgttga catgtctatg attgaccaca ttccagatat gattgaaaat ggtgtggaca
     2281 gtctaaaaat cgaaggacgt atgaagtcta ttcactacgt atcaacagta accaactgct
     2341 acaaggcggc tgtggatgcc tatcttgaaa gtcctgaaaa gtttgaagct atcaaacaag
     2401 acttggtgga cgagatgtgg aaggttgccc aacgtgaact ggttacagga ttttactatg
     2461 gtacaccatc tgaaaatgag cagttgtttg gtgctcgtcg taaaattcct gagtacaagt
     2521 ttgtcgctga agtggtttct tatgatgatg cgacacaaac agcaaccatt cgtcaacgga
     2581 acgtcattaa cgaaggggac caagttgagt tttatggtcc aggtttccgt cattttgaaa
     2641 cctatattga agatttgcat gatgccaaag gaaataaaat cgaccgcgct ccaaatccaa
     2701 tggaactatt gactattaaa gtcccacaac ctgttcaagc tggagacatg gttcgagctc
     2761 ttaaagaggg gcttatcaat ctttataagg aagatggaac cagcgtcaca gttcgtgctt
     2821 aatgtagttg tttagtttta aaaaattatg caaagctcca tatacaacac ttaaacgaga
     2881 ttaaagaatg gcgaaatccc ctgatgcgca agagattagc tgtctttttt attttttaag
     2941 tgataaagtc ggagcttagg tatcaaagcc tatcaaatta aacaaagaag cgatgtctta
     3001 gatattttga aaaaaattaa taagcagaaa actct
//
LOCUS       DAVLRT010000128         2929 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLRT010000128 DAVLRT010000000
VERSION     DAVLRT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.128"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(140..673)
                     /locus_tag="VXM85_002013"
     CDS             complement(140..673)
                     /locus_tag="VXM85_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1346525.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(762..1538)
                     /gene="recX"
                     /locus_tag="VXM85_002014"
     CDS             complement(762..1538)
                     /gene="recX"
                     /locus_tag="VXM85_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1346526.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQVSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1576..>2929
                     /gene="rlmD"
                     /locus_tag="VXM85_002015"
     CDS             1576..>2929
                     /gene="rlmD"
                     /locus_tag="VXM85_002015"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1346527.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          921 a          534 c          532 g          942 t
ORIGIN      
        1 tacgaattct gcataaatct ctttctagta atgtgttgta actctgctat aatggattta
       61 ttcctttttg tgtttacaca atttatttta tactaccaaa aaaacaaggt caggattttg
      121 ttcctgacct ttgacaactt taccgattct ttagttctac atagcgcttg taccaaatgt
      181 ttacataggc ttctgagaaa ggaccacgtc cattgttaat ccaatcaaca agaattttga
      241 catgttcttt taaaatatag tccaagtcat cagaataatt cattttgcgt ttgtgacgct
      301 cgtattcttc aacgtccaag agacgttttt ccccatctgt aaaaatttta acatccaaat
      361 cgtaatcaat atacttcagt gcttcttcat ccagatagta ggggctagcc atattgcaat
      421 agtaagaagt tccattatca cgaatcatgg caatgatatt aaaccaatat ttcttgtgaa
      481 agtaaacaat agccggttct cgagtgaccc aacgacgacc atcactttcg gtaacaagtg
      541 tatgatcgtt gacaccaata atggcgtttt ctgttgtttt tagtaccatg gtgtcccgcc
      601 aagttcggtg gagactccca tcatgcttat aactttgaat tgtaataaag tcgccttctt
      661 ttggaagctt cataactaac caactttcta caatttataa gtttatcatt tactattgta
      721 ccataaaatt acctaaaatc tgtgaatttc acttggaaat attaaagata ttctctaaga
      781 gcgcttgcta tatccgaaaa atcgtagccc tttcgtgcta aaacttgagt taaacgctgc
      841 ttcagttcgt atccttcata ctttcgggca tacttagtat attgcttatc aagttccttg
      901 aagatgagtt cctgagtcgt ttcttggtca acttgactat ccaattcgtc aaaggcaatt
      961 ttagcatcaa aataagagaa gcccttgtta gtcaagttct ggataatctt atcttgcagg
     1021 gcacgagctg gaagttttcc ctcatatttt ttcaatagtt tattggctac acgttgagca
     1081 acttccgaaa aatcaaaatc attcaagatt tcttctatag tagattttga aattcctttt
     1141 tgtgctaatt tctgagtcag tacataaggt cccttgtctc ctgaaacttg attggcattg
     1201 atgatagcat aagcgtactg accatcatta atccacttat cttctttaag attagcaata
     1261 acttgagaaa cgatgttttt atcaatatcg tattttttca gatattctct gacttctttt
     1321 tcagtgcgtg ctttaaagga taagtggtag agggccagat tcttaccata agaaaattga
     1381 gcaaagtctt gaatctcttt caattcctct tcgcttatca ccttatctct cgataacata
     1441 aaacgaacaa ttgtatcttc ggtgatatag catttgtcgc cattatcaag ctccatcaga
     1501 tagagtcttt ttttcttttc aagttttgtg attttcatag ctctattata actcaaaatg
     1561 tgataagata ggggtatgaa tctgaaagtg aaacaaaaaa taccattaaa aatcaagcgc
     1621 atgggaatta acggtgaggg aatcggcttt taccaaaaaa cattagtctt tgtacctgga
     1681 gctctcaaag gcgaagatat ctattgtcag attacttcta ttagacgcaa ctttgttgaa
     1741 gcaaaattac tgaaggtcaa caagaagtct aaatttcgaa ttgtgccatc ttgtactatt
     1801 tataatgaat gcggaggctg ccaaatcatg cacctgcatt atgataagca gctggagttc
     1861 aagacggact tacttcatca agcgctgaaa aaatttgctc ctgcaggata tgaaaattat
     1921 gaaattcgtc caactattgg aatgcaggaa ccaaaatatt acagagctaa gttacaattt
     1981 cagactcgaa aatttaaaaa tcaggtcaag gcgggcttat atgcacaaaa ctctcactat
     2041 ttagtagagt tgaaagactg cctggtacaa gataaggaaa cccaagtgat tgctaatcgc
     2101 ttagcagaat tacttactta tcaccagatt ccaatcacgg atgagagaaa agttctaggt
     2161 gtccgtacta ttatggtccg acgcgcgaga aagaccggac aggttcagat tattattgtt
     2221 acaaaccgcc agcttaattt aactcaattg gtaaaagagt tggttaaaga tttcccagaa
     2281 gttgtgacag tagctgttaa tacaaataca gctaaaacca gtgagatata tggtgaaaag
     2341 acagagatta tctgggggca agagagtatt caagaaggtg tactcaatta tgaattttca
     2401 ctatcccctc gagcttttta tcaactaaat cctgagcaaa cagaagtcct ctatagcgaa
     2461 gcagtaaaag cgctggatgt tgataaagaa gaccatttga ttgacgctta ttgtggagtt
     2521 ggaacgattg gatttgcctt tgcaaagaaa gtaaaaacac tcagaggtat ggatattatt
     2581 ccagaagcta ttgaagatgc caagcgaaat gctaaaagaa tgggatttga caatactcat
     2641 tatgaagctg gaacggcaga agagattatt cctcgttggt acaaggaagg ctaccgagca
     2701 gatgctttga ttgttgaccc accacgtaca ggtctggatg ataagttatt agatactatt
     2761 cttacttatg taccagaaaa aatggtttat atttcttgta atgtttcgac cttggctcgt
     2821 gatttggtac gcttagtaga agtctatgat cttcattata tccagtcggt cgatatgttc
     2881 ccacatacag ctcgaactga agctgttgta aaattaataa aaaaagttt
//
LOCUS       DAVLRT010000129         2890 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLRT010000129 DAVLRT010000000
VERSION     DAVLRT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.129"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            61..777
                     /locus_tag="VXM85_002016"
     CDS             61..777
                     /locus_tag="VXM85_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1346528.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKAQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELLKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(817..954)
                     /locus_tag="VXM85_002017"
     CDS             complement(817..954)
                     /locus_tag="VXM85_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346529.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEIADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            1086..1511
                     /gene="rplK"
                     /locus_tag="VXM85_002018"
     CDS             1086..1511
                     /gene="rplK"
                     /locus_tag="VXM85_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1346530.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            1719..2408
                     /gene="rplA"
                     /locus_tag="VXM85_002019"
     CDS             1719..2408
                     /gene="rplA"
                     /locus_tag="VXM85_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1346531.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT          975 a          543 c          644 g          728 t
ORIGIN      
        1 aagattagtc aaacgtttat cgttagaagt agataaacta ttagaaataa aggagtagaa
       61 atgaaaaaaa gatacctagt cttgacagct ttgctagcct tgagtctagc agcttgttca
      121 caagaaaaag caaaaaatga agatggagca gctaaaacag aacaaacagc taaagctgat
      181 ggaacagtag gaagtaaggc tcaaggagct gcccagaaaa aagcagaagt ggttaacaaa
      241 ggagactact acagcatcca agggaaatac gatgaaatca tcgtagccaa taaacactat
      301 ccattgtcta aagactacaa tccaggtgaa aatccgacag ccaaggcaga attgctcaaa
      361 ctcatcaaag caatgcaaga ggcaggtttc ccgattagcg atcattacag tggttttaga
      421 agttatgaaa ctcagaccaa gctctatcaa gattatgtta accaagatgg gaaggcagca
      481 gctgaccgtt actctgcccg acctggctat agtgagcacc agacaggctt ggcctttgat
      541 gtgattggga ctgatggtga tttggtgaca gaagaaaaag cagcccaatg gctcttggat
      601 catgcagctg attatggctt tgttgtccgt tatctcaaag gcaaggaaaa ggaaacaggc
      661 tatatggctg aggaatggca cctgcgttat gtaggaaaag aagctaaaga aattgctgca
      721 agtggtctca gtttggaaga atactatggc tttgaaggcg gagactacgt cgattaatac
      781 tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac ctactgactg cgtcggttct
      841 attcacaacc tcaaaacagt gttttgagct gacttcgtca gttttatctg caatctcaaa
      901 gctgtacttt gagcagcctg cggctagctt cctagtttgc tctttgattt tcattgagta
      961 taaaaaataa acttttctct tgcaattcca gataaatagt gtataatgga tgggtatgtg
     1021 aaaaacatac ttgtgggagg taaaaatctc taattaccgc caaaaccaca aaggaggatt
     1081 taaaaatggc taaaaaagtc gaaaaacttg taaaattgca aatccctgct ggtaaagcta
     1141 caccagctcc accggttgga cctgctcttg gtcaagctgg tatcaacatc atgggattca
     1201 caaaagagtt caacgctcgt acagctgacc aagctggtat gatcattcca gttgttatct
     1261 cagtttacga agacaaatca tttactttcg ttacaaaaac accaccagct gctgttcttt
     1321 tgaaaaaagc tgcaggtgtt gaaaaaggat caggtacacc taataaaact aaagttgcta
     1381 cagttactcg tgcacaagta caagaaattg cagaaactaa gatgccagat ttgaacgcag
     1441 caaacgtaga gtctgcaatg cgtatgatcg aaggtactgc tcgttctatg ggattcactg
     1501 ttgttgacta atcaataaca cagtagaaaa tctcacagca gtctattagt tgcttttcat
     1561 actaggcaag tgactgagcc ttgtacttgg gtacagcaag ggagcttaag gccgtaggaa
     1621 gagaaaaata gtagactgaa aacccgcaag acttcatcat ttcgagaagt gacgtgggag
     1681 atgaaaatcg attgaaccac ttacaaggag aatagaaaat ggctaaaaaa agcaaacaac
     1741 ttcgtgctgc tcttgagaaa atcgacagca caaaagcata cagtgtagaa gaagctgtag
     1801 cacttgcaaa agaaactaac tttgcaaaat ttgacgcaac tgtagaagtt gcttacaact
     1861 tgaacatcga cgttaaaaaa gctgaccaac aaatccgtgg agcaatggta ttgccaaacg
     1921 gtactggtaa aacttcacgt gttcttgttt tcgcacgtgg tgcaaaagct gaagaagcaa
     1981 aagctgctgg tgcagacttt gttggtgaag atgaccttgt tgctaaaatc aacgacggtt
     2041 ggttggactt cgacgtagtt atcgctacac ctgatatgat ggctcttgtt ggacgtcttg
     2101 gacgtgtcct tggaccacgt aacttgatgc caaaccctaa aactggtact gtaacaatgg
     2161 atgttgctaa agcagttgaa gagtctaaag gtggtaaaat cacttaccgt gctgaccgtg
     2221 caggtaacgt tcaagcaatc atcggtaaag tatcatttga agctgaaaaa ttggttgaaa
     2281 actttaaagc tttcaacgaa acaatccaaa aagcaaaacc agctacagct aaaggaactt
     2341 acgtaacaaa cttgactatc acaactactc aaggtgttgg tatcaaagtt gacgtaaact
     2401 cactttaatc agtagttaaa gtaatgtaaa aaagttgaag acactatgtc tcaacttttt
     2461 ttgatgtacg acgggcatgt cgtatatctg aggtgtaagt cctcggtggg cacctactac
     2521 cggtgaaccc aatagcgatt cccaagtctg actatcgtga ggtagcggat taaaacggtc
     2581 tggggataga ccgttttaag tctgacgctg gaaataagaa tcgtcagagg aagggatagc
     2641 gaaatcgtgg ctctacgaac aggaacgtga taataaggcg tatatagcgg ataaggtatc
     2701 tggctggggc gaccactatc aattataata aaatgaaata agaataggac gaatcgttca
     2761 ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca
     2821 atctactata gtagctcaga agtcggtact taaacgtgct atatcaaaac cagtccttga
     2881 aaaacgtgga
//
LOCUS       DAVLRT010000130         2592 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLRT010000130 DAVLRT010000000
VERSION     DAVLRT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.130"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..212)
                     /locus_tag="VXM85_002020"
     CDS             complement(<1..212)
                     /locus_tag="VXM85_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346532.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSDDLIFTWKSPSLVKEG"
     gene            complement(226..939)
                     /locus_tag="VXM85_002021"
     CDS             complement(226..939)
                     /locus_tag="VXM85_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1346533.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1398..1778)
                     /locus_tag="VXM85_002022"
     CDS             complement(1398..1778)
                     /locus_tag="VXM85_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1346534.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            complement(2200..>2592)
                     /locus_tag="VXM85_002023"
     CDS             complement(2200..>2592)
                     /locus_tag="VXM85_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1346535.1"
                     /translation="DHVQRNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKP
                     ESPKPTEEPEEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLK
                     NNLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          787 a          508 c          417 g          880 t
ORIGIN      
        1 tccccttctt tgactaatga tggagatttc caagtaaaaa ttaaatcgtc agagaaattt
       61 ttcttatcaa aaaacttaaa aagagattga taaatactat ttgctaattg acaagtgtca
      121 gatgtaaatt cataattaac agcttcatta aaatcaactt cagacatttc aatgtactct
      181 tcaatagtaa tattagcttt ttttgtagtc attagctaat ctcctttaaa atgtgatctt
      241 ttcaagaata tttttatcta aacaatccag caagtcttgg taagaataga cttcgtaagt
      301 cggctgggct tgtgtgtgat tttcgaggtg atgaggatta taccagatag tgtcaatccc
      361 cgcattattg ccaccttgaa tgtcggcggt tagagaatct ccaatcatca gcgtcttttc
      421 tttactaaat ccagcaattt gctggccaat cttttcataa aagagagcat ccggcttttg
      481 agtttgcaac tgttctgaga taaagacttg attgaaataa ggtactagac cagattgagc
      541 caaacgtcct gtctgaatgg cagtaatgcc atttgtcgca gcatacaagt tataatcacg
      601 ctcaatgagg ctgtccaaga gatcatgagc gcccgatagt gtttgtccct gctgagcgag
      661 ataaaattgg taacgctggg caagaaaact accgtctttt tcctgtccaa aatgagaaaa
      721 taaacgagaa aagcgcgtgt taaccagctc ttgtttactg attttcttca gctccaagtc
      781 tttccagaga gccttgttca taggaacgta ataatcttta taagcctgaa tatccgcaac
      841 tccttcttct tttagaagtt gggtcaaagc cacatcctca gcagcatcaa aatcaagaag
      901 agtgtggtcg aggtcgaaga gtagaaattt gtagaacaat ttgaggtttt cctttctgaa
      961 aattcattaa gaacattata tcataaagca cctcatacaa ttaactaatt taatcactta
     1021 aaaaaaattc gaacactttc tatacaactg acagctcaaa tctttcagaa tagaacaata
     1081 ctaactatcg agcaccctgt cttcataaat atatgtgtaa ttctagacct agaattccta
     1141 taaactaaat gctttcatac tcttccaagt aattgattgc cttaaatttt aatttttgaa
     1201 ggattctaaa gctagaatag tcccatcaca atcagttttg attgattcac aatttagaaa
     1261 cactatagtt tcactcctgt taaaataaaa aggaactgca taaagcaatc cctttctgat
     1321 tttgaaatca tttacttaac attttatagt tgagataatc aatagcttac ctataaaaag
     1381 agttatagta aaattcctta tttattgatt tcaagctccg ctaactgtgt ttgaataact
     1441 gacagttctg caccagcctg aagaagagca gctgcattat aggcaccttc tacaattgga
     1501 accctgttga tgatgatact tttatcactg aaatcagtca ccatttttaa gttcatttta
     1561 gcagaaccta ggtcaaaaaa ggcaagtaaa gtatctgctg gattttcgga aacaacccta
     1621 tctacttgat caaaactcgt tccaattcct ccgccctcgg ttcctcctac ataagtaatc
     1681 ggaacatctt tagctacttt actaatcagt tcaacaacac cttctgcaat gtgtttggaa
     1741 tgtgaaacga taacaagacc aataccaata ctttccatca aaccacccca gtttctaaaa
     1801 tagcagtaaa gagtaatcct gatgagaatg atccaggatc aatatgtcca agaaaccaca
     1861 tgctcctaag acaagagcta acagactggc catcaataat agtattgttc tttttttcat
     1921 cattactcct taactagtgt ttaactgatc aattagccag taaatagttt atctttattt
     1981 acactatctg ttaagatata gtaaaatgaa ataagaacag gacagtcaaa tcgatttcta
     2041 acaatgtttt agaagtagag gtatactatt ctagtttcaa tctactatat tttgcacatt
     2101 ttcatgaaaa aaatgagaac tagaactcac attctgctct catttttcgt tttcccgttc
     2161 tcctatcctg tttttaggag ttagaaaatg ctgctacctt tacttactct cctttaataa
     2221 agccaatagt ttttcagctt ctgccataat agtattgttg tcctgggtgc caaatagtaa
     2281 attatttttt aatcctgtga gagtctcttt ggcattggac ttgataattg gatcctggat
     2341 ttttccaagt aaatcttcag cctctctcag tttttcttca accttttcag tctcgacctg
     2401 aggttcttct gattcctctg gtgattcttc ttctggttcc tctgttggtt ttggagactc
     2461 tggtttctcg ctttgcggtt tctcttctcg aggggtttct tcctcaggtt tttctgtctg
     2521 aggtttctcc tcgcttggtt tttccgtttg attggtatca gcttgaccat ttttgtttct
     2581 ttgaacatgg tc
//
LOCUS       DAVLRT010000131         2320 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLRT010000131 DAVLRT010000000
VERSION     DAVLRT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.131"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            44..1798
                     /locus_tag="VXM85_002024"
     CDS             44..1798
                     /locus_tag="VXM85_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346536.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNEGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1764..>2320
                     /locus_tag="VXM85_002025"
     CDS             1764..>2320
                     /locus_tag="VXM85_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346537.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVIIPSILSFIFIPLSKKY"
BASE COUNT          813 a          318 c          429 g          760 t
ORIGIN      
        1 gaatttatat ctaaaaggga tattaaagaa aggagatatg cttatgaaga tttacaaaaa
       61 actatttgct tatgtccaag ataagaaata tcttggggtt ttggccataa ttttttctgc
      121 tatatctgct ctacttacag tatatggata ttatttaatc tacaaatttc tagataagtt
      181 aataattaat tcaaatttat ccggtgcaga gagtatagca ttaaaatctg ttattacact
      241 aacaagtgga gcgatatttt attttgtctc aggaatgttt tcacatatct tgggattcag
      301 gcttgaaaca aatttaagaa aaaggggaat cgatggtctg gaaaaagcaa gttttaggtt
      361 ctttgactta aatccatctg gtcaaataag aaagatcata gatgataatg cagcacaaac
      421 tcatcaggtg gtagcacaca tgattcccga tagttctcag gcaataatca cacccgtact
      481 tgtacttgca cttggcttta tagtaagtat aagagttggc ataattttgc ttgctcttac
      541 tataattggt ggcttaattt taggggcgat gatgggcgag caagaattta tgaagatata
      601 ccaagaatcc ctatctaaac taagtgctga aactgttgag tacgtgagag gaatgcaagt
      661 tgtaaaaata tttaaagcaa atgtagagtc atttaaaagc ttttacaagg ctataaaaga
      721 ttactcaaag tatgcttatg attattccct atcttgtaaa aggccttatg ttttgtatca
      781 atggttattt tttggactga ttgcaatttt aattattcct atagtttatt ttatgactag
      841 cttagctagc gcaaaggtga ttttacttga gcttatcatg attttatttt tatcaggagt
      901 tctctttgtt tcattcatga gaatgatgtg gtactccatg tatatttctc aaggaaatta
      961 tgcagtagat actttagagg cgctttacga agatatgcaa aaagacaaat tagtgcatgg
     1021 taatgtcaat aattttaaaa actataatat agaatttgag aatgttagct ttgcttataa
     1081 tgataaagct gtcattgaaa atttatcctt taatttagaa gaaggaaagt cctacgcact
     1141 tgtcggttca tctggatcag gcaaatcaac agtagcaaaa cttatatcag gtttttacaa
     1201 tgttaatgaa ggaagcataa agataggcgg gatagcaata agtgaatatt ctgacgaagc
     1261 cttaattaaa gccatttcct ttgtttttca agattcaaaa ttattcaaga agagcattta
     1321 tgataatgta gctttagcta ataaagatgc gacgaaagat gacgttatga gagccttaaa
     1381 attagcagga tgcgatttaa tattagacaa attcccagaa agagaaaata caatcatagg
     1441 ctcaaaaggt gtttatttat ccggtggaga aaaacaaaga attgcaattg ctagagcaat
     1501 tttaaaggat tccaaaatta ttattatgga tgaagcatca gcatctattg acccagataa
     1561 cgagtttgaa ttgcaaaaag cttttaaaaa tcttatgaag gataaaacag ttatcatgat
     1621 tgcacacagg ctatctacaa ttaaagacct tgatgaaatt attgtcatgg atagtggaaa
     1681 aattatagaa agagggtctg acaaagaatt aatgtcaaaa gatacaaggt ataagagcct
     1741 gcaagagatg tttaacagtg cgaatgaatg gagggtttca aatgaaagag ttttataaaa
     1801 aaagatttgc tcttacagat ggaggagcaa gaaatttaag taaagcaaca ctggcttcat
     1861 ttttcgttta ttgtataaac atgcttcctg ccatattact tatgattttt gctcaggaag
     1921 ttttggaaaa tatgggcaaa agcaatggct tttatatagt attctcagtt ttgactttga
     1981 tagcaatgta tattttgctt tctatcgaat acgataaatt atataacaca acctatcaag
     2041 aaagtgcaga tttaagaata aggacagcgg agaatttatc aaaattacct ctatcttact
     2101 tttctaaaca tgacatttcc gacatttcac aaacaatcat ggctgatatt gaaggcatag
     2161 agcatgcaat gagccactca ataccaaagg tgggcggcat ggtactgttt ttcccattaa
     2221 tatctgtaat gatgctagcg ggcaatgtca agatgggttt agctgtaatt attccatcta
     2281 ttttaagctt tatatttata cctttatcta aaaaatatca
//
LOCUS       DAVLRT010000132         2220 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLRT010000132 DAVLRT010000000
VERSION     DAVLRT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.132"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..569)
                     /locus_tag="VXM85_002026"
     CDS             complement(<1..569)
                     /locus_tag="VXM85_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV1346538.1"
                     /translation="MSTRNSLIKNSLYNVAYRMLNIIFPLVSATYTARIILASGVGEV
                     SFAQNVVSYFTTIAAMGIPNYGIREIAKTRNKAEATNKVFSELFLINGLSTLLCSIVY
                     LILILTFNSIFDNTSMYLAVGLTLVFNFLNIDWFYQGKEDYAYITKRSFSIKLISLFS
                     LFIFVRDTDDAVNYALISSLAIGGNNIFNL"
     gene            complement(574..1581)
                     /locus_tag="VXM85_002027"
     CDS             complement(574..1581)
                     /locus_tag="VXM85_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346539.1"
                     /translation="MFNINDQSIIYVACPYFNKTGGTELVHQLVYSINHFGGKAIVAY
                     YGSESAPNKVNPAFREYVSTFVDIKDVIDSLDNIIILPEINPDLAANLESIQKAVWWM
                     SVDNYLKRNGILGSLEHFGLLRTIKLFVKGNIKIGGYRIDKEIPHLYQSEYAKQFLLA
                     KGVKKYYRLSDYLNESYIQRSISTDTKKDVILYNPKKGIEFTRELIKQAPELTFSPIE
                     NMSTEQVKKLLSKSKVYIDFGNHPGKDRFPREAAISGCCVITGKRGSAKYYKDIPIPD
                     SYKFEDTVENIPNIIETLKSCLKNYDKHTVDFEKYQQYIKSEHDLFERDVKELFTRNR
                     G"
     gene            complement(1574..>2220)
                     /locus_tag="VXM85_002028"
     CDS             complement(1574..>2220)
                     /locus_tag="VXM85_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV1346540.1"
                     /translation="NRFSEKTEKKRKVSKIKIVNYGMLGSIILWLSTLSRTTTSVYRI
                     LYFYFAMSPILLEKWSSVLDSEKLVTKGLVSLNGFFFSISYVLKNLFRIDYSQRVLEA
                     YTMIANTDAIWYNIAPGLTKANAYVSLFWFFYADGRLLGVLIGSLLYGTFCGYIFCRY
                     IQQQNKKNLAMLLFIYQGVFFSFIRFPFSKSNYAIAFVLLLFFAFKKKGIEKSV"
BASE COUNT          793 a          391 c          303 g          733 t
ORIGIN      
        1 agattaaaaa tattattccc gccaattgct aagctagaaa tcaatgcata gttaactgca
       61 tcgtcagtat ctctaacaaa aataaataaa ctaaaaagag aaataagttt tatactgaaa
      121 ctacgtttcg tgatgtatgc atagtcctct tttccttgat agaaccaatc gatattaagg
      181 aagttaaaca ctaatgttaa acccacagca agatacattg atgtattatc aaaaatgcta
      241 ttgaatgtca aaatcagaat taaatatact atagaacaca gtaatgtaga caatccatta
      301 atcagaaata gctctgaaaa aactttattc gtagcctctg ctttatttct agttttagca
      361 atttctcgta taccataatt tggaataccc atagcagcta tcgttgtaaa gtaagaaaca
      421 acattttgtg caaacgagac ctccccaact ccactagcta aaattatccg tgcagtatat
      481 gttgcggata ctaatggaaa tatgatattt aacattctat aagcaacatt ataaagagaa
      541 tttttaataa gtgaatttct ggttgacata accttaaccc ctattcctag tgaataattc
      601 tttcacatct ctttcaaata aatcatgttc agatttaata tattgttgat atttctcaaa
      661 gtcaacagta tgtttgtcat aattttttaa gcatgatttc agggtctcaa taatgttggg
      721 gatattttca acagtgtctt caaatttgta tgaatctgga ataggaatat ccttataata
      781 ttttgcagaa cctcgtttcc cagttatcac acaacaacct gatatcgctg cctcccgtgg
      841 gaatctatct ttcccaggat ggttgccaaa atcaatatat actttgcttt ttgaaagtaa
      901 ttttttcacc tgttcagttg acatattctc aattggagag aacgttaatt ctggagcctg
      961 tttaattagc tcacgagtaa attcaatccc ttttttagga ttgtaaagaa taacatcttt
     1021 tttagtgtct gttgaaatag atcgctgaat ataagactcg tttaaataat cagacaatct
     1081 atagtacttt ttgacacctt tagctagaag aaattgcttg gcgtattcac tttgataaag
     1141 atggggaatc tctttatcta ttctatatcc acctatttta atatttcctt ttacaaataa
     1201 tttaattgtt cgtaaaagtc caaaatgttc caaggaacct aaaataccat tacgcttcaa
     1261 ataattgtca acgctcatcc accatactgc tttttgaata gactctagat tagcagctaa
     1321 atccggatta atttccggta aaataataat attatcaaga gagtcaataa catcttttat
     1381 atcaacaaac gtagaaacat attctctaaa cgcaggattc accttatttg gagcactttc
     1441 actaccataa taagcaacga tagctttccc cccaaaatga ttaatagaat aaactaattg
     1501 atggacaagc tccgttccac ctgttttatt aaaataagga catgcaacat aaataataga
     1561 ctggtcattg atattaaaca cttttctcta ttcctttctt cttaaaagca aaaaataata
     1621 agagtacgaa tgcaattgca taatttgatt ttgaaaacgg aaatctaata aaagagaaaa
     1681 aaacaccttg ataaataaat agtaacatag ccaaattctt tttattttgt tgctggatat
     1741 acctgcaaaa gatataacca caaaaggtac catatagaag ggatcctatt aaaactccta
     1801 gtaaacgtcc atccgcataa aagaaccaaa acagagagac atatgcattt gccttggtca
     1861 aaccaggtgc aatattatac cagattgcat cagtattcgc aatcattgtg tatgcttcta
     1921 atactctttg cgaataatct attctaaaca gatttttaag tacatatgag attgaaaaaa
     1981 agaaaccgtt caacgaaacc aaaccctttg ttactaattt ttcagaatca agtacactac
     2041 tccacttttc taataatatt ggggacattg cgaaataaaa ataaagaatc ctataaacgg
     2101 aggttgttgt cctagataaa gttgatagcc ataagataat tgaacctagc ataccataat
     2161 ttactatctt aattttacta acttttctct tcttttcagt tttttcagag aacctatttg
//
LOCUS       DAVLRT010000133         1995 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLRT010000133 DAVLRT010000000
VERSION     DAVLRT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.133"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            447..1922
                     /locus_tag="VXM85_002029"
     CDS             447..1922
                     /locus_tag="VXM85_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1346541.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVVELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          592 a          367 c          456 g          580 t
ORIGIN      
        1 tgttttttat caaaaaatac tttacaagtt cttaaaaaca tgatatagta ataaagctta
       61 gaaaatgaga tgatgttttc tagcaaatat aaacccgagt aaaaaatgcc tacggacagg
      121 cagggttgaa tgccgaagcg tggttgaaaa gccacattat tgatagggtt aaaagcctac
      181 ttttataagt tgatgttagg acacttgtcc taattcataa atttttagtg tggtgaaagc
      241 acacgtcatc ttgtgaaacg atcaataaag tacgtaatat ttgctactag agagttagga
      301 aacatcggga acagacatac tcaacagaaa ccaaaataaa cacgtcagaa gattgcagag
      361 caggtgaaaa cctgctcttt tttcatgagt caacctttag ttccttagtt ttcataaggt
      421 cctaaaaata ttgaaaggag tatgttttga aagagttaga tcaaaaccaa gccccaattt
      481 atgaggcctt ggtgaagtta cgcaagaaaa ggattgttcc ctttgatgtt ccaggtcaca
      541 agcgtggacg gggaaatcca gaacttgtcg aactcttagg agaaaaatgt gtaggcattg
      601 atgtcaattc gatgaaaccc ttggataatt taggccatcc tatttcgatt attcgtgatg
      661 cagaggagct ggctgcagat gcttttggag ctagccatgc ctttctaatg attggtggaa
      721 caacttcatc ggtgcagact atgattctgg caacctgcaa ggcaggagat aagattattc
      781 tgccacgaaa tgtccataaa tctgctatca atgcgttggt tctatgtggt gccattccca
      841 tctatatcga gatgagtgta gatcctaaga ttggtatcgc tttaggtctt gaaaatgacc
      901 gagtagcaca ggccataaag gatcatccag atgctaaggc tatcctaatc aacaatccta
      961 cttactacgg catctgttca gacctaaagg ggttgacaga aatggctcat gaagctggca
     1021 tgatggtttt agtagatgaa gcccatggag cgcatttgca tttcactggt aaacttccaa
     1081 tttctgctat ggatgcagga gctgatatgg cagcagtttc catgcataag tctggtggga
     1141 gtttgacaca aagttcgctt cttttaatcg gggagcagat gaatcccgag tacgttcgtc
     1201 agattataaa cctgacccag tctacatctg cctcttactt attgatggct agtttggata
     1261 tttcacgtcg caacttggcc cttcgtggta aagagtcgtt tgaggaagtc gttgagctat
     1321 ccgagtacgc tcgtcatgaa atcaatgcca ttggcggtta ctatgcttac tcaaaagagt
     1381 taatagatgg tgtgtcggtc tgtgattttg atgtaaccaa gttgtcagtt tacactcagg
     1441 gaataggctt aacaggtatc gaggtttatg acctcttgcg agacgaatac gacattcaga
     1501 tcgagtttgg tgatatcggc aatatcttgg cttatatttc catcggcgac cgcatccaag
     1561 acatcgagcg cttggttggt gctctggctg atattaagag actctattca cgagatggga
     1621 aggacttaat agctggagaa tatattcagc ccgagttggt gctgtctcca caagaagctt
     1681 tctattcaga gagaaaaagt ttgaccttag atgagtctgt tggacaggtc tgtggagaat
     1741 ttgtcatgtg ctatcctcca gggattccta tcttggctcc tggtgaacgc attacacgag
     1801 aaattgtaga ctatatccaa ttcgccaagg aacgtggttg ctccctccaa gggacggaag
     1861 atccagaggt caatcatatc aacgttatta agagaaagac aaactataag aaaagtcaat
     1921 agttttatct aaactatttc ttatttcaat ttgatgattt ggcgatgatt ttagagcacg
     1981 gcaaaaagcc cttga
//
LOCUS       DAVLRT010000134         1900 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLRT010000134 DAVLRT010000000
VERSION     DAVLRT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.134"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..883
                     /locus_tag="VXM85_002030"
     CDS             <1..883
                     /locus_tag="VXM85_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEV1346542.1"
                     /translation="MYYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYF
                     IVLSIIEDYDTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRR
                     GIEKERVLRLVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYK
                     ELILQLVPREKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFY
                     IDYMKEMDTSMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYI
                     ALNQNDKKIVIPSLLEI"
     gene            1233..>1900
                     /locus_tag="VXM85_002031"
     CDS             1233..>1900
                     /locus_tag="VXM85_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEV1346543.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEAN"
BASE COUNT          693 a          229 c          342 g          636 t
ORIGIN      
        1 tatgtactat gtaaaattat tgaaagataa atcatcatat actcaactgt acaataaaat
       61 gttatctgca attccgccat ctgtttcttt atctaagacc acatttggtt taactgggga
      121 tataggatat ttcattgttc tatccattat agaggattac gatacaaata ctctcgcatg
      181 cttaaattat ataaagagtg ttttaactga gcttgaaaag actgattttg caagtgtttc
      241 aaacaagtct gactatatta atggtcttct acctttaatt aatacactgg taagatatta
      301 taggagagga atagagaagg aacgtgttct aaggctagta ttaagtttag gtgatacgct
      361 atataatgat gtaagcgaga gagataatag taattttgga tatagttttg gccatggact
      421 atcaggtgta atttttacat taaaaaatat ttataaagtg acaagactta aaaaatataa
      481 agaattaatt ttacaactag ttcctaggga gaaaatcaaa attcattctt tgaagtggtg
      541 tagtggagaa tttgggacat tagttaatca tccccaaagt gttccagagg gaattgtagc
      601 tagtttagat aatgatacat tttgccatgg aagtatggcc ataataaatt tttacatcga
      661 ctacatgaaa gaaatggata ctagtatgga attggagtct ttattgtgta gtgttgtaat
      721 gaataaatca aaagaaggag agtatcgtat cgtccaaaca agcaatttcc cagatttttc
      781 tttgtataca ggtgtgactg gtattgctta tactttttta aggtatatcg cactgaatca
      841 gaatgacaaa aagatagtta tcccctcatt acttgaaatc tagcttaatt tatagttttg
      901 tttctaaaat agaggtgtcc actcattaag caatataaaa ataccaagat tgactagttt
      961 aatatgtaat tctataaaat atttttaata gtgtagcttt tcgttgagat aataatataa
     1021 taaagaaagg ttctattcta attgctaaac ggcttgaggt atatgctact gttatagaaa
     1081 tgattttaaa ctaaaatcaa aattactcca aaaatagtat tgattttggt ttaatacaaa
     1141 acatatgtaa taatgtgagt caacaaaagt ctgtttttgt accatactat cttgaaaaat
     1201 ctactaaatt caaattgaga ggatgaatta aaatgaataa aatatttata tatgcaggag
     1261 tgcgaaatca taactctaag actttagagt atactaagag actttcaagt atcatttcta
     1321 gtagaaataa tgttgatata tctttcagga cacctttcaa ttcagagcta gaaatatcta
     1381 attctgattc agaagagttg tttaagaaag gaattgatag gcaaagtaat gctgatgatg
     1441 gaggtgttat aaaaaaagag ttattagagt ctgatattat aattatctct tctcctgtat
     1501 atttacaaaa cgtatctgtg gacacaaaaa actttattga aaggattggg gggtggagcc
     1561 atctttttcg attagctgga aagtttgttg taacattaga tgttgctgag agtaacggct
     1621 ctgataatgt gtcagaatat cttagagata tattttcata tatgggagga cagatattac
     1681 accaagtttc aattaccaat tcactcaaag atattgcaga ggctcagttg atggaggcaa
     1741 cttataaaat tgaagatgta cttgaaggga aaattaagta caaaacaacg gattatcaag
     1801 aaagagctta ccagacatta aaattaattt tagaaaatta tgattccgaa cattttgaga
     1861 agatgtattg ggagaaaaaa cgcctttttg aagctaatag
//
LOCUS       DAVLRT010000135         1865 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLRT010000135 DAVLRT010000000
VERSION     DAVLRT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.135"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..537
                     /locus_tag="VXM85_002032"
     CDS             <1..537
                     /locus_tag="VXM85_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346544.1"
                     /translation="PLISFSIIIALVQKLYRIENQQLFLLKSKVLTILESKKNCEFNL
                     QDYHEIWKLQSKSELPCVALSYISLIKPYLSESVREQIDLLEVKRFKKINNPISLYGM
                     LDVIKLNLYLRHYNEKNKYESMLKKILEVRPDFVLIEQNIDDSLNSSQPLSLSLAISE
                     IQLLLEVYIGIKHVSIRR"
     gene            914..1288
                     /locus_tag="VXM85_002033"
     CDS             914..1288
                     /locus_tag="VXM85_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV1346545.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            1496..>1865
                     /locus_tag="VXM85_002034"
     CDS             1496..>1865
                     /locus_tag="VXM85_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346546.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPLDFVVSTSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVF"
BASE COUNT          630 a          228 c          334 g          673 t
ORIGIN      
        1 cctcttattt cattttctat tatcatagca ttagtacaaa aactttatag aattgagaat
       61 caacaattat ttctgttaaa atcaaaggta ctaacaatac tggaatctaa gaagaattgt
      121 gagtttaatc ttcaggatta tcatgaaatt tggaaattgc aatcaaaatc tgagttgcca
      181 tgcgtggctt tatcttatat cagtttaatc aagccatatc tttcagagag tgtgcgtgaa
      241 caaattgatt tattagaagt taagcgattt aaaaaaataa ataatccaat atcactttat
      301 ggtatgttgg atgttataaa attaaatcta tatttacggc attataatga aaaaaataaa
      361 tatgaatcaa tgttaaagaa aattttagag gtaagaccag actttgtttt aatcgagcag
      421 aatattgatg atagtttaaa tagctcgcaa ccattatctt tatctttagc catttcagaa
      481 atccaacttt tgcttgaagt gtatataggg atcaaacatg tttcaattag gagataaaac
      541 tagaataata tttgccatta taggggcaag ttgcatatta gtcagtatgt atcttattcc
      601 acttattaaa actgagaata aattttggga aatactttta tttatattag gtgtattgct
      661 aattggaatt gcgttactaa gtcaaaaaaa ataaattttg aaatagtatt agtgagttcg
      721 caatcattta ttgctagtgg tttagaactg ttaagtttca aacaataatt gattttaaac
      781 cttaggtaac ctataaaaac aaataaggta gagaaaggat atcttatcta ggtagctaag
      841 tgataaaaac acagtcgtct atcacttgtt aggaaacgat cctaactatg cttaataaaa
      901 aaggagaaaa taaatggaac tcgtattacc aaataattat gttgctcttg agcaagaaga
      961 gatgatgtat cttgatgggg gattttctat tctgagatgg cctgttgcaa cagccattaa
     1021 tatagctttt aatggtgttt taggtggagg agcaatcagt ctagttagaa attatattcg
     1081 taattatggt ttgcggcgag ttacaagcgc aattgctgga gcagctgcaa gatatgttgg
     1141 ggtacgagtt gcaaatagag tggcaggatt tgcactgtct gctattaatg gatttgcagc
     1201 ttggatgtca attggcgatg ctattacaac aatctgggcc aacaatgatg taaataggag
     1261 agacccaaat ttaaacgcct tgtggtaact agatatgggt attaaaatgg aaaaaatttt
     1321 tgttattatt ttttttgtat gtctatttat atcatccata atttttttag cctatgattt
     1381 tgttagcgaa gaaataaaaa agttgattat ttggatgaat gtcgtttttt tgattttaat
     1441 catagcaatg ataatttatc caaaattaag aaaatgataa ggacactgta aaattatgat
     1501 acgtaaacta atcatttttt tattaatgct ccctcttttt gtcttgtggc tagagttgca
     1561 tttgttagtg agtaacttgc aattgaatct agaaattccc cttgattttg tggttagtac
     1621 gagtcttgct ttctgtagtt tgattttatc aaaaatagtt ttagacatac tatacgcatt
     1681 gaaagatttg tacaagaaag aagctcttat tacgattttt ccttttattt ttataggcag
     1741 gaagaaagta aatgtgaggt tctcgcccta tttttcattc catcgtaaaa gtttgtcgcc
     1801 tgatgattta agatctagga ttatatggag ttttattctt gaaattgcta ttattctagt
     1861 attta
//
LOCUS       DAVLRT010000136         1751 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLRT010000136 DAVLRT010000000
VERSION     DAVLRT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.136"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..>1751
                     /locus_tag="VXM85_002035"
     CDS             <1..>1751
                     /locus_tag="VXM85_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEV1346547.1"
                     /translation="ELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYITKEFTGKLLSS
                     EGKQFAITELEHPLFDKIKAGTVKNINFENIDINVSNQDNIATIANEINGTTRIENIK
                     ATGTITARNNVAGFVNNIKENAALENVAFIGNINSVGNNSTVGGIAGSNYMGFVNKAY
                     VDANITAANASASMLVPLVTYMLNSYQYGTKSKVTNSVAKGTLDVKNTRFVGGVVAKT
                     WPWGVIKDIVTYAKVIKGEEVYGSNEINDGDARQFLSDIYRVNGASSGANNFTNLQKI
                     KQLTKEQADAKVATFNITADKLESELSPLAKLNEEKAYSSIQDYNAEYNQAYKNLEKL
                     IPFYNKDYIVYQGNKLNKEHPLNTKEVLSVTAMNNNEFITNLDEANKIIVHYADGTKD
                     YFNLSSSSEGLSNVKEYTITDLGIKYTPNIVQKDNTTLVNDIKSILEPVDLQSQTMYQ
                     HLNRLGDYRVNAIKDLYLEESFTAVKKNLTNLITKLVQNEEHQLNDSPAARQMLRDKV
                     EKNKAALLLGLTYLNRYYGVKFGDVNIKELMLFKPDFYGEKVSVLDRLIEIGSKENNI
                     KGSRTFDAFGEVLAKYT"
BASE COUNT          681 a          271 c          289 g          510 t
ORIGIN      
        1 aagaattagt ggaagctatt caaaacgatc cttcaaaaga atatcgtctg ggacaatcaa
       61 tgagcgctag aaatgttgtt cctaatggaa aatcatatat cactaaagaa ttcacaggaa
      121 aacttttaag ttctgaagga aaacaatttg ctattactga attggaacat ccattatttg
      181 acaagattaa agctggaact gttaaaaata taaattttga aaatattgat attaatgtgt
      241 ctaatcaaga taatatcgcg acaattgcga atgaaatcaa cggaaccaca cgtatagaaa
      301 acatcaaagc aactggaacg ataacagcca gaaataacgt tgctggattt gtaaacaata
      361 tcaaagaaaa tgctgctcta gaaaacgtag catttatagg taatatcaat tctgttggta
      421 ataattctac agtcggagga attgctggtt cgaactacat gggatttgtt aataaagctt
      481 atgttgatgc taacattact gccgctaacg caagcgcaag tatgctagtt cctttggtta
      541 catatatgtt aaatagctat cagtatggaa ctaagtcaaa agttaccaat tcggttgcta
      601 aaggaacttt agatgttaaa aatactcgtt ttgttggtgg tgttgtagct aaaacttggc
      661 cttggggagt tataaaagat attgttacct atgctaaagt aattaaaggt gaagaagtat
      721 atggttcaaa cgaaattaat gacggagatg cacgccaatt cttatctgat atttatagag
      781 tcaacggcgc tagcagtgga gctaataatt ttaccaatct gcaaaaaata aaacaattaa
      841 ctaaagaaca agctgatgct aaagttgcta ctttcaatat tactgctgat aaattagaaa
      901 gtgagctatc tcctcttgca aaacttaatg aagaaaaagc ttattctagt attcaagatt
      961 acaacgctga atataatcaa gcctataaaa accttgaaaa attaatacca ttctacaata
     1021 aagattatat tgtataccaa ggtaataaat taaataaaga acaccctcta aatactaaag
     1081 aagtcctttc tgttacagcg atgaacaaca atgagtttat cacaaaccta gatgaagcta
     1141 ataaaattat tgttcactat gcagacggta caaaagatta ctttaactta tcttctagca
     1201 gtgaaggttt aagtaatgta aaagaatata ctataactga cctaggaatt aaatatacac
     1261 ccaatatcgt gcaaaaagat aacactactc ttgttaatga cataaaatct attttagaac
     1321 cagtagatct tcaatctcaa acaatgtatc aacacctaaa tcgattaggt gactatagag
     1381 ttaatgcaat caaagattta tatttagaag aaagcttcac agccgttaaa aagaacttaa
     1441 caaacctaat cacaaaatta gttcaaaatg aagaacacca gttaaatgat tctccagctg
     1501 ctcgtcaaat gcttcgtgat aaagtcgaga aaaacaaagc tgccctatta ttaggtttaa
     1561 catacttaaa ccgttactac ggtgttaaat tcggtgatgt taatattaaa gaattaatgt
     1621 tattcaaacc agacttctat ggtgaaaaag ttagcgtatt agacagatta attgaaatcg
     1681 gctctaaaga gaacaacatt aaaggttcac gtacattcga cgcattcggt gaagtattag
     1741 ctaaatatac t
//
LOCUS       DAVLRT010000137         1699 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLRT010000137 DAVLRT010000000
VERSION     DAVLRT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.137"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(101..250)
                     /gene="blpO"
                     /locus_tag="VXM85_002036"
     CDS             complement(101..250)
                     /gene="blpO"
                     /locus_tag="VXM85_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1346548.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(494..697)
                     /gene="blpN"
                     /locus_tag="VXM85_002037"
     CDS             complement(494..697)
                     /gene="blpN"
                     /locus_tag="VXM85_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV1346549.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(713..967)
                     /gene="blpM"
                     /locus_tag="VXM85_002038"
     CDS             complement(713..967)
                     /gene="blpM"
                     /locus_tag="VXM85_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV1346550.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            complement(1117..1699)
                     /locus_tag="VXM85_002039"
                     /pseudo
     CDS             complement(1117..1699)
                     /locus_tag="VXM85_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          520 a          394 c          239 g          546 t
ORIGIN      
        1 taccaccaac atgtcgctgc ataggccaca cctccaagta tagctcctcc cgcagcacca
       61 gttacaccta ttcctatagc aaatggtccc aatagaaatg tcaaaccgtt gttgcacacc
      121 catcaattgc gccatatgca acccctgctg cacaactaat ttttcttccc caatcaatat
      181 ctccaccttc aacgcaagca agcatttcat tatccataac tgcaaattgt gacatcattt
      241 ttgtatccat atagtatatc acttttcagt tacggaacaa gtttaatata aaaattatca
      301 aaaaaacata ggcaataaag agaaaaatta atttatcata gattagaaat aatatgacaa
      361 aacaattcaa tgatgttaat tcaatagtct tttgtttttt atcggagata cttatggata
      421 gataaataag ataggtttga aaagcgaaga gaataataaa gaatatagcc ttcataaaat
      481 ttagctttca tttttatgat gtagcggtat aggctaaata tccacaaacc actgctcctc
      541 caattcctcc tattgcagcg ccccatggtc ctagaagtct cccatatttc actccacccg
      601 ctgcacaacc taaagcagca actacagctg ctcctccgga attacctcca taaacctcac
      661 tcagcattgt ttcatttata ttacaataag tattcataca agtctccttt tattaaaatc
      721 cacccgttgc ccctgttact cctgcccaaa gatccacacc aaatttagct cctatgtatc
      781 cacatgctcc cataaatggt gctccaacac cactcgcagc acaaatagct gtccctagcc
      841 cccagccacc aaaagcagca ccaccacctt ctaagacatt agtttgccaa ttattcttgc
      901 ctccttcaat actagataac atagttatat ccatttcatg aaattgttcc ataatttttg
      961 tatccatgac aaatactctt ttttattttt aatttttgtc ttgttgtaac tttgacaagt
     1021 ttagtatatc atcgtttttt aaaatttttc atccagatct tgaatagtca tcgaaacgtc
     1081 ttgaattgca aaaattacat tagacttcct gcaaaactag aatcctagtt catgattgat
     1141 aataccagca atcaaattca ttcgtaatcc gaagcgttta cgatgatttc gataggttgt
     1201 tgaaaacatt ttaaacgttt ttactttggc aaagatgttc tcaaccttgc ttctctcctt
     1261 agatagcgca tggttacagg ctttatcttc aactgttagc ggtttgagtt tgctggattt
     1321 acgtgaagtt tgtgcttgag gatatatctt catgagccct tgataaccac tgtcagccaa
     1381 gattttacca gcttgtccga tatttctgcg actcattttg aacaacttca tatcatgaca
     1441 atagttcaca gtgatatcca aagaaacaat tctcccttga cttgtgacaa tcgcttgagt
     1501 cttcatagcg tgaaatttct ttttaccaga ataattcgct aattcttttt ttagggcgat
     1561 tgatttttac ttccgtcgca tcaatcatta ccgtgtcctc agaactgaga ggagttcttg
     1621 aaatcgtaac accactttga acaagagtta cttcaaccca ttggctccga cggagtaagt
     1681 tgctttcgtg aacaccaaa
//
LOCUS       DAVLRT010000138         1693 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLRT010000138 DAVLRT010000000
VERSION     DAVLRT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.138"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            107..634
                     /locus_tag="VXM85_002040"
     CDS             107..634
                     /locus_tag="VXM85_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1346551.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            646..1161
                     /locus_tag="VXM85_002041"
     CDS             646..1161
                     /locus_tag="VXM85_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1346552.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHQVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(1158..1625)
                     /locus_tag="VXM85_002042"
     CDS             complement(1158..1625)
                     /locus_tag="VXM85_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1346553.1"
                     /translation="MSRSQLTILTNICLIEDLETQCVVMQYRAPETNRWSGYAFPGGH
                     VENGEAFAESVIREIYEETGLTIQNPQLVGVKNWPLDTGGRYIVFCYKATEFTGTLQS
                     SDEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
BASE COUNT          474 a          369 c          313 g          537 t
ORIGIN      
        1 tataactttt aactaaaaaa tgatctataa acccaagtaa attcttgctt tattgtctgg
       61 tttattgtac catactagtg tatatgcagt taaaaaggag attcttgtga atacacggaa
      121 aaagacacaa tttatgacaa tgacagccct tttaacggct attgcgattt tgattccaat
      181 tgttatgcct ttcaagattg tcattccacc tgcttcctat actttgggga gccacatcgc
      241 tatttttata gccatgttct tgtcgccctt gatggcagtt tttgtcatcc tagcctctag
      301 ttttggattt ttgatggctg gctatcccat ggttatcgtt tttcgggctt tttcccatat
      361 atcttttggt gctttaggag ctctttacct acaaaaattc cccgataccc tagataaacc
      421 aaaatcttcc tgggttttca attttgtttt ggctgttgtt catgcccttg ctgaagtatt
      481 agcctgtgtc cttttttacg caacttcggg taccaatgta gaaaacatgt tctatgttct
      541 atttgtacta gttggatttg gcacaattat ccatagtatg gtagactata cattagcact
      601 agctgtctat aaagtgcttc gaaaacgccg ttaaaaggaa aagctatgac aaaagatcgc
      661 aaacaagccc tgctccaact cttgaaagaa gctcctaaag cccttaatgg ccaaagtttg
      721 gcggaacatt ttcatgtcac acgtcaggtc attgtgcagg acattgcaat tttgagagcc
      781 gatggcgctc ctatcctatc caccaatcgt ggctacatct ataagcaaat tgataccaat
      841 ccttatgttc acaaactttt caaagtgaaa catcaagttg aagaaatcgg tcaggaactc
      901 cttgctattg tcgataatgg tgggcatgtc cagaatacct tgattgacca tcccgtttat
      961 ggagaaattg aaaccttgct taaactgtct tgtcgcagag atgtgcaaca ttttctagaa
     1021 caagtcgagc attctgactt tagacctcta tctgaattga cagatggcat ccattaccac
     1081 ctagtcgaag ccgaaacaca acaagacctc cactatatcg aggaagcctt ggatcagcta
     1141 ggttatttag taaaagacta gaagattttc ttttcccaat cgtcttctgt acggcgaggg
     1201 tagaaaaact ctgacttgtc gggagcttcc atcatttcca tcaatggtag catatcatag
     1261 gccagattca agtttggaat ctggtctttt tgcacccaag aaacttctcc ctcgtctgaa
     1321 gattgaagag ttccagtaaa ctcggtcgcc ttgtaacaaa agacaatgta gcgcccccct
     1381 gtatccaaag gccaattttt aacacctacc agttgcggat tttggatagt caatcctgtt
     1441 tcttcgtaga tttcacgaat gacagactcc gcaaaagcct caccattttc tacatgacct
     1501 ccaggaaagg cataaccaga ccagcgattg gtttcaggag cacggtactg cataaccacg
     1561 cactgagttt caaggtcttc aatcagacaa atatttgtta aaatcgttaa ttgggaacgg
     1621 gacataaatt tactcgcttt ctaatttatt aaattttcag acttggtacc agtgccatta
     1681 gcttctactt ttt
//
LOCUS       DAVLRT010000139         1690 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLRT010000139 DAVLRT010000000
VERSION     DAVLRT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.139"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            24..710
                     /locus_tag="VXM85_002043"
     CDS             24..710
                     /locus_tag="VXM85_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1346554.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            711..1643
                     /locus_tag="VXM85_002044"
     CDS             711..1643
                     /locus_tag="VXM85_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1346555.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
BASE COUNT          555 a          227 c          335 g          573 t
ORIGIN      
        1 ataaaaaaac ggaggttttc tgaatggaaa ctaaaaaaat taaaaattta aaaggtcaaa
       61 taattgtttc ctgccaagcg ttagaaggag agccacttta tactccaaat ggaggagtga
      121 tgccgttatt agcaaaagcc gcctttcaag caggcgctaa aggtattcgt gctaattctg
      181 tccgcgatat ttcagaaata aaagaagaag ttgacttacc aattataggt atcataaaaa
      241 gagattacga tggctttgaa ccttttattt cagcaactat gaaagagatt gatgaattag
      301 ttagtgaagg agtagacatt ttagctttgg attgtactaa tcgttcaaga ccaggttatg
      361 ataatattac tgactttatt catgatatta aagtcaagta tccaaaccaa ttattaatgg
      421 ctgatatttc tacttttgaa gaagggaaag tagctgctga atcgggagta gattttgtgg
      481 gtacgacatt atctggttat acaccatata gccctaaaaa agataatcct gattttgagt
      541 tagttgaaag attggtaaaa gagcttgacg taccagttat tgctgaagga agaataagta
      601 ctccggaaca agctagaaaa atgttagatt taggtgctta cgcagtggtt gttggaggag
      661 ctatcactcg tcctttggaa atagcgaaga agtttattga ggtagtttag atgaatcaag
      721 aaatattaaa aaagagtaag attcaattga cagttgaaca tattcaaaaa tattggattt
      781 tatatttaat gatgattcca gcaactgttc tactaatttt atttacgtat ggaccaatgt
      841 acggcattat tatggccttt caagacttta ctgtcttcaa gggatatact ggaagtccat
      901 ttgtcggttt aaaacatttt caaagattgt tctcagatcc tttgttttat agattgttca
      961 agaatacgtt catggtaggg gttttagact ttttattctc tttcccagct ccgcttattt
     1021 ttgcactcat attaaacgaa gtcagaaaag taagatttaa gagtgtagtt caatctatta
     1081 gttatcttcc tcattttatt cccctagtgg ttatggttgg tattattttt gaactatttg
     1141 gttcatatgg tattattaac agtttacttt catcacttgg aatggagcca ataagtttct
     1201 ttacaaaatc tgaatggttc ttgcctctct acattggttc aggagtatgg aaaacaatag
     1261 gttggggttc tatcatttat atgggagcat taaccaatat agattcaact ttatatgaag
     1321 cagcagatat ggacggggct aatagatggc acaaaatgtg gcatgtaact ctaccaagtt
     1381 taagacctac agtagttact ttgtttatac taaatgctgg tggtatcatg caagtaggat
     1441 ttgaaaaggt attcttgatg tctagtcctg caacatatga agtatctgat gtattatcta
     1501 cttatgttta tagacaaggt attttaaatt ctgatttcag ttactcagct gcagtaggat
     1561 tatttaataa catagtagct ttgttatttg ttttattagc taataagatt gctaaaaaac
     1621 ttggcgaaga aggaattgta taaggggggt aaaatgattt tttcaaaaaa tacaaaaaag
     1681 agtaatagag
//
LOCUS       DAVLRT010000140         1667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLRT010000140 DAVLRT010000000
VERSION     DAVLRT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.140"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            1..555
                     /locus_tag="VXM85_002045"
                     /pseudo
     CDS             1..555
                     /locus_tag="VXM85_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            570..1310
                     /locus_tag="VXM85_002046"
     CDS             570..1310
                     /locus_tag="VXM85_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346556.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            1313..>1667
                     /locus_tag="VXM85_002047"
     CDS             1313..>1667
                     /locus_tag="VXM85_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346557.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFI"
BASE COUNT          585 a          207 c          323 g          552 t
ORIGIN      
        1 caccatttag ggtattatta acaggaactt ctggtagtgg gaaaacaacg attttaaatt
       61 taattaatgg ttctttaaag ccacaaaaag gttatgtaaa tttgttatca catgggaaaa
      121 agagttcaga ttcaatacca acagttgatc agacaccata tatttttgac actactattc
      181 gtgagaacgt aactttattt caaaatgaat atttttcaga tgatcagata attgaggtgt
      241 taaaaaaggt aaatctatat gaagaattag aaaagataga tatactaaat tatcaatgtg
      301 gtgaaaatgg tagtaatttg tctggaggtc aaaaacaaaa aatagcttta gctagagctc
      361 tgattagaaa taataaagtg tacttatttg acgaaatatc agctaattta gataatgata
      421 attcaaattc catacatgat attctgttca atttaggtat ttcatttatt gaagtttcac
      481 atcattatga cttaaatgac aagagataca ctgatatata aattggaaaa tggaacactt
      541 ttcaaaatta agtgattgga ggcaagaata tggataaaga ttatatatta aaagtgaaag
      601 ggctgtatca tcaattttta ctgggaaaca ataaaacgtt gcaagtgctg aaaaatgttt
      661 ctctttctgc ttcgagagga gaatttataa gtattctagg aattagtggt tctggaaagt
      721 caactttatt aaaatgtatt tctagtttgc ttgaaccaac aagtggggaa gtaattttaa
      781 atggaatcaa cccctataaa atcagaaatg caaaattgtc aagtataaga cgtaacgaag
      841 tatcttttat atttcaagca tacaatttaa taccttccct gccggtaata gaaaatatag
      901 cacttccttt gcgattatca caaaaaaaat taactattaa aaatgtagaa aacttactca
      961 aaagaatgaa gtttaatgct ggcttaaacg attttgttgg aactctgtct ggtggagaac
     1021 aacagaaggt tgctatagct agagcggtta ttgctgatag tgatataata tttgctgatg
     1081 agccaactgg ggctttagac agcgtttctc gtgaagtaat ttttgaatta ttgagagagt
     1141 tagtaggggc gggtaagtgt gtaattatgg taacgcacga tatagaattg gcctcgaaaa
     1201 ctgatcgtgc attaatattg aaagatggaa aaattttcaa agaacttcat agacctagcg
     1261 gggaagagtt gtataaaatc ttagaggtac aatcaactac ggaggaatag agatgctcag
     1321 attagtttac tatcaatttt tacataataa gaaacaatgg ttaggcgttt caccagtaat
     1381 ttttgtttcg agtttagtta tgggattggc tgttaatggg gtaattaatg ttgagaataa
     1441 ttcgcaagta tttgtcggac ttccagatcc taaaccaatt tttatgtttc cgatagtatt
     1501 cggaggagta acattattct ttgtgctctc taacataata aatatgttgg ttgaaatttt
     1561 tagagatgat tacgaacttc tggaggtgtt gggagcaagt ctccttcaac tatcatttct
     1621 tgtgggggga cagattttta tcatttcctc gattattagt tttattg
//
LOCUS       DAVLRT010000141         1656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLRT010000141 DAVLRT010000000
VERSION     DAVLRT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.141"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..318
                     /locus_tag="VXM85_002048"
     CDS             <1..318
                     /locus_tag="VXM85_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEV1346558.1"
                     /translation="LPATANVIGKIANGIADDLVTATVLNFNKKIIFCPNMNSTMWDN
                     HIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDSTCSMLQPQSLVKELIKLENIVLE
                     EGH"
     gene            539..1645
                     /locus_tag="VXM85_002049"
     CDS             539..1645
                     /locus_tag="VXM85_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV1346559.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGSA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
BASE COUNT          607 a          205 c          290 g          554 t
ORIGIN      
        1 ttaccagcta cggcaaatgt gattggcaaa attgctaatg gtattgctga tgatttagtt
       61 acagcaactg ttttaaactt taataaaaaa ataatttttt gtcccaatat gaactctact
      121 atgtgggaca atcacatagt tcaaagaaat gtatcaattc taaaggagtt gggacatata
      181 tttttatttg agtctaaaaa aacatatgag gtaggattgc gtaaagcaat agattcaaca
      241 tgttcaatgt tacaaccaca gtcgttagta aaagaactta tcaaattaga aaatattgtc
      301 cttgaagagg gacattaaaa actactgaga atattaagga ggggaaaaaa tggaaaattc
      361 atcaatcgat gtagatatgc tgttggaaga attgacacaa gaagcaatgg tcgttgttgc
      421 tgttgataag gactgttaat ttaaacttat ggcaatatat gaaaggttac tggatgtttt
      481 aaattatgca ggcagtagcc ttttattata tacaaatgga taaagtaagg ataatacaat
      541 gattaataaa aaaatacaac aagttgtttt ggaatcatta cagaattttt tgaatgggaa
      601 cttcatttcg ccttgtgtag tctatgattt tggcttgctg gaaactgtac ttgatgaatt
      661 taaaaatcaa attcctgtaa cattcaatta ccaacttttt tatgccgtta aagcaaattc
      721 aaatgagaag atacttgaat tcttagtaga taaaattgat ggagttgatg tggcgtcatt
      781 atctgaatta gatgtggcta aaaaattttt cccaccaact caaatttctg ttaatggttc
      841 cgcattttct tatgaaactt tatataatct gattaaaaaa caatataaag ttgatattaa
      901 ctttttggaa catcttcaac aattttcccc aaaagaatct gttggaataa gagtaacgga
      961 gccagatgaa cttaataatc gtatgagtcg atttggaata aatatttgca gtgataattg
     1021 gactagtaat ttacaaaatc ctttaattac acgactgcat tttcattttg gagaaaaaga
     1081 tgataaattt attgttaagt tagataaaat attatttaag ttacaagaaa ttaataaact
     1141 tagagaggtt agagaaataa atcttggagg cggttttatg aaattattta tggaaaatcg
     1201 tttgaaagaa ttttttctat cacttatgga aatctataaa aagtacgata ttgatagtac
     1261 tgtgactaca ataatagaac caggtagtgc aattacttca ttttctgcct atatgattac
     1321 tagcccagtt aatgttagtg aggtgaatga gcagcaggtt atcacgttag acacatcaat
     1381 atacaccaat acattatggt ttgttccgca tattattaca acgttaaatt caagtagtaa
     1441 agagcgttat agtactattc tctatggtaa tacctgttat gaacatgaca agtataaaat
     1501 gaaagtttcg cttccaaggt taactcaaaa tagcagtata gtgttttttc ctgtaggagc
     1561 ttatataaaa agcaatcatt caaatttaca tcgtaatgat tttatgcggg aggtatattt
     1621 gtggacaaaa aacttgacat attagataaa gttaag
//
LOCUS       DAVLRT010000142         1652 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLRT010000142 DAVLRT010000000
VERSION     DAVLRT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.142"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..1262
                     /locus_tag="VXM85_002050"
     CDS             <1..1262
                     /locus_tag="VXM85_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEV1346560.1"
                     /translation="KIMHWLILSISACILFSTIVNIRAPTFVQILFIFLGGFIIYKIH
                     LKIKIFRYDLEHYLIIRESLLYVLHTNRLYTTSKDGSGYEKIVRSAVLEYEIDRQKGH
                     VLIKALITGDEFSKKVQSLDDVLSDVLELELEEKIIRPSFIEYHFYYIKPKRLVLQSH
                     SQRQEIDSLDIDLGYGVNYNPVKCSHILVSGGTGSGKSVLISVLILEFLKRQSTVYIA
                     DPKNSDLGSLSHYFGNKYVATTPNNIARIVRVVVEEMQERYQVMRDNFQYGSNFTNHG
                     FKPVWLIFDEMGAFQATGSDKKSREIITEVMDGIKQIILLGRQSGIFILVSAQQVNAS
                     TTLSTELRDNLGLRIALGANSSEGYHMVFGSATPDKLKPIEEKGSGYLYIQGSGKESA
                     QYWESPYLDTTQFDFIKELQLYVTKSK"
     gene            1352..>1652
                     /locus_tag="VXM85_002051"
     CDS             1352..>1652
                     /locus_tag="VXM85_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346561.1"
                     /translation="MKLNDFLKPELLGNKFIAVKGYTEVLDRETNKPVALRLNVSIQD
                     EDSDFFMEMIQVKVNTLSPTASVQEMANNKTCPVTLKDLNVGQYNGNLWFSCSNVI"
BASE COUNT          568 a          267 c          289 g          528 t
ORIGIN      
        1 taaaaataat gcactggctg atactaagta tctctgcctg cattctattt tccactattg
       61 ttaatattag ggctccaact tttgttcaaa ttctcttcat atttttaggt ggatttatta
      121 tctataaaat tcatctgaaa ataaaaattt ttcgatatga cttagagcat taccttatta
      181 tacgtgagag cttactttat gtactccaca caaacagact ttataccaca tctaaagatg
      241 gttcaggcta tgaaaaaatc gttagaagtg cagtattaga atacgagata gatagacaaa
      301 aaggtcatgt tcttatcaaa gctcttataa cgggcgatga atttagtaaa aaagttcaat
      361 ctcttgatga tgtactttct gacgtgcttg aactagagct tgaggaaaag attattcgac
      421 ctagttttat agaatatcat ttctattaca taaaaccaaa aaggttagtt ctacaatctc
      481 acagccaaag acaagagata gatagccttg atatagattt aggatatggt gtaaattata
      541 atcctgtcaa atgctcacat attttagtat cgggtggaac tggaagtggg aaatccgtgc
      601 ttatatctgt tttgatttta gaatttctaa aacgtcaatc aacagtttat atagctgacc
      661 ctaagaactc tgacctaggt agcttgtctc attactttgg aaataaatac gtagcaacca
      721 ccccaaataa tatagctcgc attgtgagag tcgtagttga ggaaatgcag gaacgatatc
      781 aagtcatgcg tgataacttt cagtatggtt caaattttac taaccatgga tttaagccag
      841 tatggcttat atttgatgaa atgggagcat ttcaagctac aggttcagat aaaaaatcaa
      901 gagagattat cactgaagtt atggatggga ttaaacaaat cattctactt ggaagacagt
      961 caggcatttt tatcctagtg tctgcccaac aagtaaatgc tagtactact ttaagtactg
     1021 aactcagaga taatttagga ttgagaattg ctcttggtgc taattctagt gaaggctatc
     1081 acatggtctt tggttcggct actcctgata agttaaaacc tattgaagaa aaaggttcag
     1141 gctatcttta tatacaaggc tctggtaaag aatctgccca atactgggaa agcccttatc
     1201 ttgatacaac tcaattcgat tttataaaag aactacaatt atacgtaaca aagtcaaaat
     1261 aatttcaaca gatatttttg cttaaattta atagattttc agcaaaaata tctgttcctc
     1321 atcgctctgc gatgatagaa aagaggtaca catgaagcta aatgattttt taaagccaga
     1381 actgcttggc aacaaattta tagcagtaaa aggctataca gaagtattag acagagagac
     1441 taataagccc gtagctttac gacttaacgt gtcaattcaa gatgaggatt ctgatttctt
     1501 tatggagatg atacaagtca aagtaaatac attatcccct actgcttctg ttcaagaaat
     1561 ggcaaataat aaaacctgtc ctgttactct aaaagattta aacgtaggtc aatacaatgg
     1621 aaatttatgg ttttcttgtt ctaatgtaat tc
//
LOCUS       DAVLRT010000143         1632 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLRT010000143 DAVLRT010000000
VERSION     DAVLRT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.143"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            77..484
                     /locus_tag="VXM85_002052"
     CDS             77..484
                     /locus_tag="VXM85_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1346562.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQTDEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            649..948
                     /locus_tag="VXM85_002053"
     CDS             649..948
                     /locus_tag="VXM85_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1346563.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1209..1532
                     /gene="tnpB"
                     /locus_tag="VXM85_002054"
     CDS             1209..1532
                     /gene="tnpB"
                     /locus_tag="VXM85_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1346564.1"
                     /translation="MIIQLSDLGQVHLVCSKIDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDDQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          552 a          325 c          288 g          467 t
ORIGIN      
        1 taataacgat actagataat gaggaaaatg caatggcaaa cccaactctc ggagaaaaaa
       61 agcgaataca gactatattg ctcgctatgg cgtatatgca gttatcccta atcctgaaca
      121 aaaacaaatt gttcttgttc aagaaccaaa tggtgcttgg ttcctaccat gtggagaaat
      181 tgaagcaggt gaaaatcatc aggaagccct aaagcgtgag ttgattgaag agcttggttt
      241 cacagcagaa attggtacct attacggaca aactgacgaa tatttctatt ctcgtcatcg
      301 tgatacctac tactacaatc ctgcctacct ctatgaagca acttctttca aagaagtaca
      361 aaagccacta gaaaacttta atcatattgc ctggttccct attgacgagg ctatccaaaa
      421 ccttaaacgt ggtagccata aatgggccat tgaatcttgg aaaaaacagc ataagattgg
      481 ctaatactct tcgaaaataa aattcaaacc acgtcagcgt cgccttaccg tactcaagta
      541 tagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa atcaagcgac
      601 tttattcaaa aagtgctata atagaattag aaaatcggag gaaatattat gaagcttatc
      661 aatacgacaa actcacactc gcaacttgta aaaagtcaac tagaaagtac cgatgcaacc
      721 cttgttgagg tttattctgc agggaataca gatgttattt ttacccaagc tccgcttcac
      781 tatgaaatcc tcatctcaaa caaacaccgt gctattcgtg agcctgaaat cgaaaccatt
      841 caagaatttt tcttgaaacg taaaattgat aaggcctctg ttgatgaagc caatatcaag
      901 acactctact cagagaaatt aattggaatt tcaatcccaa tcaagtaaga aaattgagta
      961 aaaactcgat ttgagagaaa gtccaataag tctttccata acaaaacgca tagtaacaag
     1021 agtttctaca cccactatta tacgttttta tcattttgaa atctgctgct aatcaatgaa
     1081 gaagcaaaga tcaaactaag cagctagtta caagttacgt aagcgcgtca taacaaggta
     1141 tctatcctaa ttccccaact tccagtatac tagataaaaa ataattatcg gaggaaaggt
     1201 attgtactat gataattcaa ctaagtgatt taggtcaagt tcaccttgtt tgtagcaaga
     1261 tagatatgag gcagggaata gactcattag cctatgtagt taaaacccac tttgaattgg
     1321 atcctttctc cggtcaagtc tttctctttt gtggtggacg taaagaccgc tttaaagccc
     1381 tttactggga tgatcaagga ttttggctac tatataaacg ctttgagaac ggcagactga
     1441 cttggcccag tacagaaaag gatgtcaaag ctctcacacc tgaacaagta gactggctta
     1501 tgaagggctt ttctatcact ccccaaatat agtggattta aactagaata gtacacctct
     1561 gcttctaaaa cattgttaga aatcgatttg actgtcctga tcgatttgtc ctgttcttgt
     1621 ttcattttac ta
//
LOCUS       DAVLRT010000144         1604 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLRT010000144 DAVLRT010000000
VERSION     DAVLRT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.144"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            262..>1604
                     /locus_tag="VXM85_002055"
     CDS             join(262..703,703..>1604)
                     /locus_tag="VXM85_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1346565.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKEIQASMSRKGNSPDNGMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKS"
BASE COUNT          558 a          259 c          356 g          431 t
ORIGIN      
        1 agttatggat gtcgttccaa tatgtgcacg ttggaatgtt agtgcttata tggggggaac
       61 agaatcctct cttgattgaa gacaagttag ccattaggct ggtttgtctc tttgtcaact
      121 gtagtgggtt gatataatag tattagtgag tgggataaaa gtttcattta gtttattcag
      181 tacaaattta acgggtcaag atttattgaa ctgcacccca atagttagac agaaaaaatc
      241 taacttttgg ggtgttttat tatgaaattg agttatgaag ataaagttca gatctatgaa
      301 ctaagaaagc aaggacaaag cttcaaacag ctttcaaaaa gatttggtgt ggatgtttct
      361 ggtctaaagt acatggtgaa attaattgac cgttatggca tagaaatcgt taaaaaaggg
      421 aagaatcgtc actattcttc taaattgaaa caagaaatga tggataaagc cttactggaa
      481 ggttgttcac aaagaagtat tagtcttgat tatgccctcc caaaccaagg aatgctttca
      541 ttttggccgg cacaatacaa gaaaaacggg tatactattg ttgagaaaac aagagggaga
      601 ccagctaaaa tgggacgtaa acggaagaag acttgggaag aaatgacaga actagaacga
      661 ctccaggagg agaatgaacg cttacggact gaggtggcct acctaaaaaa gttaaaagag
      721 ttagaggaaa gggacgaagc cctagagcga gaaaggcaga gacagttaga gaaatggttt
      781 caggaggatt tcgactagat tttcttcttg aaacagcacg tttagctcgc tcgacttact
      841 attatcagtt gaaacaactg gatggggttg ataaagataa agagattaaa accgaaattc
      901 agggcattga taatgaacat aaaggcaact atggctatcg tcgaatccat ttagaactaa
      961 ggaatcgtgg ttttgtggtc aatcataaga aagttcaacg gttgatgaga attcttggtt
     1021 taacggctcg aattcgccgt aaacggaagt attcttccta ccaaggagag attggcaaga
     1081 aagcagagaa tctcattcaa cgccagttcg aagcatcgag gccaatggaa aagtgctata
     1141 cagatgtgac agagtttgcc attccaaata gcacacagaa actctattta tcaccagttt
     1201 tagatggttt taacagtgaa atcattgctt atcatctttc tacttcgcca aacttagaac
     1261 aagtgaagag tatgttagaa caggccttta cagagaaata ctatgagaat acgattctcc
     1321 atagtgacca aggctggcaa taccaacacg attcttatca tcggttccta gagagtaagg
     1381 aaattcaagc atccatgtca cgcaagggca acagcccaga caacggcatg atggaatctt
     1441 tctttggcat tttaaaatcc gaaatgtttt atggctatga gaagaacttt agatctttag
     1501 aaaaccttga acaagctatt gtggactaca ttgattacta caacaacaaa cgaattaagg
     1561 taaaactaaa aggacttagt tctgtgcagt acagaactaa atcc
//
LOCUS       DAVLRT010000145         1582 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLRT010000145 DAVLRT010000000
VERSION     DAVLRT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.145"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            277..1413
                     /gene="dnaJ"
                     /locus_tag="VXM85_002056"
     CDS             277..1413
                     /gene="dnaJ"
                     /locus_tag="VXM85_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1346566.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGDGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGQGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1442..>1582)
                     /locus_tag="VXM85_002057"
     CDS             complement(1442..>1582)
                     /locus_tag="VXM85_002057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346567.1"
                     /translation="IKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCFEVVDTTHIYTAS
                     RR"
BASE COUNT          449 a          314 c          383 g          436 t
ORIGIN      
        1 ctaagaagag acaaaaatat ataaatattt ctgtacttat aggatattta aaatcaaaat
       61 aaagttaatt tacttatttg cagaggttgc aacccagcct ctgtttttcg ataaaaaggg
      121 atagggccga gtggctggag tattatctca tcaatataaa agaaaggaat tgaacccgac
      181 ctaaattctc ggaaaaaaga gaaatctgtc taggagcgtc gctccagcgt cagatttcct
      241 atttttcagt cgaattttac ggtcttggta tcttgtatga acaatactga attttatgat
      301 cgtctggggg tatccaaaaa cgcttcggca gacgaaatca aaaaggctta tcgtaagctt
      361 tccaaaaaat atcacccaga tatcaacaag gagcctggtg ctgaggacaa gtacaaggaa
      421 gttcaagaag cctatgagac tttgagtgac gaccaaaaac gtgctgccta tgaccagtat
      481 ggtgctgcag gcgccaatgg tggttttggt ggagatggtg gtttcggcgg tttcaatggg
      541 gcaggtggct tcggtggttt tgaggatatt ttctcaagtt tcttcggcgg aggcggttct
      601 tcgcgcaatc caaacgctcc tcgccaagga gatgatctcc agtatcgtgt caatttgacc
      661 tttgaagaag ctatcttcgg aactgagaag gaagttaagt atcatcgtga agctggctgt
      721 cgtacatgta atggctctgg tgctaagcca gggacaagtc cagtcacttg tggacgctgt
      781 catggcgctg gtgtcattaa cgtcgatacg cagactcctc ttggtatgat gcgtcgccaa
      841 gtaacctgtg atgtctgtca cggccaagga aaagaaatca aatatccatg tacaacctgt
      901 catggaacag gtcatgagaa acaagctcat agcgtacatg tgaaaatccc tgctggtgtg
      961 gaaacaggtc aacaaattcg cctcgctggt caaggtgaag caggctttaa cggtggacct
     1021 tatggtgact tgtatgtagt agtttctgtg gaagctagcg acaagtttga acgtgaagga
     1081 acgactatct tctacaatct caacctcaac tttgtccaag cggctcttgg tgatacagta
     1141 gatattccaa ctgttcacgg tgatgttgaa ttggttattc cagagggaac tcagactggt
     1201 aagaaattcc gcctacgtag taagggggca ccgagccttc gtggcggtgc agttggtgac
     1261 caatacgtta ctgttaatgt cgtaacaccg acaggcttga acgaccgcca aaaagtagcc
     1321 ttgaaagaat tcgcggctgc tggtgacttg aaagtaaatc caaagaaaaa aggcttcttt
     1381 gaccatatta aagatgcctt tgatggagaa taatactctt tgaaaatctc ttcaaaccac
     1441 gtcagcgtcg gcttgccgta tatatgtgag tcgtatctac aacctcaaaa cagtgttttg
     1501 agctgacttc gtcagttcta tccacaacct taaaacggtg ttttgagcag tctgtgccta
     1561 gctttctagt ttgctttttg at
//
LOCUS       DAVLRT010000146         1579 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLRT010000146 DAVLRT010000000
VERSION     DAVLRT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.146"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            296..1093
                     /locus_tag="VXM85_002058"
                     /pseudo
     CDS             296..1093
                     /locus_tag="VXM85_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1128..1337)
                     /locus_tag="VXM85_002059"
                     /pseudo
     CDS             complement(1128..1337)
                     /locus_tag="VXM85_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1503..1576)
                     /locus_tag="VXM85_002060"
     tRNA            complement(1503..1576)
                     /locus_tag="VXM85_002060"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1540..1542),aa:Asn,seq:gtt)
BASE COUNT          541 a          314 c          232 g          492 t
ORIGIN      
        1 tagccatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct tttttataaa
       61 cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt aaaataccag
      121 cctcaacaag ataaaaatag gaaaagttga cagaatagaa acaaatttgc tacctaaatt
      181 tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc ctattagaac
      241 tataatattt ttatataggt attatggttc tgattttcta gtataaaaaa ttcaaactag
      301 aattcttctt agaaaaggcc gttttaagtg ctatcactgt tcaaaaatga gggtcgctga
      361 aacttctatc gtcaagaaga atcatcaaat tccttgtatc atcaaccaaa agattgctca
      421 gaagctaatt aaaaagactt ctatgaccga cattgatcat cagttgtcta tttcaacttc
      481 aactgtcatt cgcaagatca atgattttca ctttgagcat gatttttcgc gtcttcctga
      541 gattatgtcc taggacgttg aaacagtccg gggagtgact gtttcaatcg ggagatggag
      601 atgagcttta ttgctcaaga ctttgataag ctcaatatca taactgttct tgagagcaga
      661 acacaagcca tcatccgaaa tcccatgaat acaaggctat caagcgatac tgaaagctca
      721 ttcaacaaga tagtcagaaa ctgagtgata aacgatttta tcgccctact tttcgtatgc
      781 acttgactaa taaagaaatt ctagataaac ttttgagcta ttccgaagac ttgaaacacc
      841 actataatct ctatcagctc ttgcttttcc acctcttttt cagactctgt ttaaaacctt
      901 cctcaaagat aaagagaaaa tcgtcaacgc ccttcaatta ccttattcca acgccaaact
      961 ggaagccacc aataatctca tcaaacttat caagtacaat gcctttggtt ttaggaactt
     1021 tgaaaacttc aaaaaagaaa ggacgaaatt tgtcctttct agatcttcgc tttcttcaac
     1081 ccactacagt tgacaaagaa ccgaaaaatg tctcgcctct aatatagtca attgaaacaa
     1141 gaacaagaca aaagagcctc ataaaaggta ttgcaacttg ctaatacctt tttggggttc
     1201 tttttgatat gaacccatgt tttctcaata ggattgtact caggtgagta gggaggaaga
     1261 ggtaaaagtt tatgcccaaa ctcttcacac aagagttcta gcttacccat tctatggaat
     1321 cttgcattgt ccataatata gtagattgaa actagaatag tacacctcta cttctaaaac
     1381 attgttagaa ttcgatttta ctgtcctgat cgatttgtcc tattcttatt tcattttact
     1441 ataacacgaa atagattatt attacataac aaaaaagagg tctaaacctc ttaactaaat
     1501 tactccgcca gtaggactcg aacctacgac atcatgatta acagtcatgc gctactacca
     1561 actgagctat ggcggatta
//
LOCUS       DAVLRT010000147         1483 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLRT010000147 DAVLRT010000000
VERSION     DAVLRT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.147"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            248..393
                     /locus_tag="VXM85_002061"
                     /pseudo
     CDS             248..393
                     /locus_tag="VXM85_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
     gene            520..1242
                     /locus_tag="VXM85_002062"
     CDS             520..1242
                     /locus_tag="VXM85_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1346568.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEKDIEIDKEREGEIDKKSTP
                     IFYGEFKNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEE
                     DKDKLRQKGLNKKMNYDVGESL"
BASE COUNT          718 a          190 c          245 g          330 t
ORIGIN      
        1 atttaaggat atcgaaagag ataaggtaat agacaaccct aaacttttta atctaacaaa
       61 ttatctatta acttattcag aaattcaaaa agaaaaaagt aaatatctaa aaggatatgc
      121 aaaaactaat aaagaaaaat atgacttcaa aaagctaaca agtgcatata gttatttaag
      181 aaaaaacaac atagaaacca taggtcagtt acaaacaaaa atagaaactt taaagtctaa
      241 tagttatagg ctaaataaaa aagaaaaaga aagtatccaa aaacaaataa agaaatacaa
      301 agacctatcc aaaaacaaag ataagacaaa agaaaaagga aagtaaaaga aaaagtaaaa
      361 ccaaacaaaa aagatatgac cagagaaatc taaagggagt aacgaaaagt tacaccctta
      421 aataaccata ataacaagac ttccgaaaaa cagaagtcca gaattccgaa aatcggaaat
      481 caagaatttc gattatcgaa aatcaagaaa ggagcagata tggcaacaaa caaaagatac
      541 tattggataa aattaaaaga agaatttttc acagacaaaa ggataaaaag gttaaggaga
      601 atatctggag gagataccta tacgataatc tacctaaaac ttctactact aagcctaaaa
      661 gatgagggca aactctatta tgacggagta gaaagtgatt ttataaaaga gttagcacta
      721 accatagatg aaacagacga tgatgtaatg gtaacagtaa attatttaat caatcaaggc
      781 ttattagaag ttgtaacaga aaatgatgaa tactacctaa ctgaaatacc aaacttaatc
      841 ggatcagaaa cagcctgggc agagaaaaaa agaagataca gacaaaataa acagaggaca
      901 ttgtccttaa tgagtccaac ccatgtccga caagagatag agaaagatat agagatagat
      961 aaagagagag agggagagat agataaaaag tccaccccca ttttttatgg agaattcaaa
     1021 aatgtaaggt taagcaaaga agaatataaa aatctaaaag aaaaattaaa ctcacacaca
     1081 gatataatga taaataaact atccagatac atggaaagca gtggtaagac ctatcaaaac
     1141 cactatgtga caatattaaa atggtatgaa gaagataaag acaaactaag acagaaaggt
     1201 ttaaataaaa aaatgaatta tgatgtagga gaatctttat agataaaaag aggtggaaag
     1261 gcactattaa agactttaag tctaaaaagg tatcaaagta tgccagagat aaaaaaatat
     1321 aaatatgaca ctttaaaggc gattagaaaa ttaaagaaat gctagaagac ttttagatga
     1381 cagttaccaa gagggtctaa ttttcactgc tacttatgca gtgggaattg aaccttttta
     1441 tataagcaaa ttctatttca aaattggtta ccaagaaaga ctt
//
LOCUS       DAVLRT010000148         1433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLRT010000148 DAVLRT010000000
VERSION     DAVLRT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.148"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            13..>1433
                     /gene="pcpA"
                     /locus_tag="VXM85_002063"
     CDS             13..>1433
                     /gene="pcpA"
                     /locus_tag="VXM85_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1346569.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWY"
BASE COUNT          559 a          196 c          235 g          443 t
ORIGIN      
        1 aaggaaattt aaatgaaaaa aactacaata ttatcattaa ctacagctgc ggttatttta
       61 gcagcatatg tccctaatga accaatccta gcagatactc ctagttcgga agtaatcaaa
      121 gagactaaag ttggaagtat tattcaacaa aataatatca aatataaggt tctaactgta
      181 gaaggtaaca taggaactgt tcaagtgggt aatggagtta ctcctgtaga gtttgaagct
      241 ggtcaagatg gaaaaccatt cacgattcct acaaaaatca cagtaggtga taaagtattt
      301 accgttactg aagtagctag tcaagctttt agttattatc cagatgaaac aggtagaatt
      361 gtctactatc ctagctctat tactatccca tcaagcataa aaaaaataca aaaaaaaggc
      421 ttccatggaa gtaaagctaa aactattatt tttgacaaag gcagtcagct ggagaaaatt
      481 gaagatagag cttttgattt ttctgaatta gaagagattg aattgcctgc atctctagaa
      541 tatattggaa caagtgcatt ttcttttagt caaaaattga aaaagctaac cttttcctca
      601 agttcaaaat tagaattaat atcacatgag gcttttgcta atttatcaaa tttagagaaa
      661 ctaacattac caaaatcggt taaaacatta ggaagtaatc tatttagact cactactagc
      721 ttaaaacatg ttgatgttga agaaggaaat gaatcgtttg cctcagttga tggtgttttg
      781 ttttcaaaag ataaaactca attaatttat tatccaagtc aaaaaaatga cgaaagttat
      841 aaaacgccta aggagacaaa agaacttgca tcatattcgt ttaataaaaa ttcttacttg
      901 aaaaaactcg aattgaatga aggtttagaa aaaatcggta cttttgcatt tgcggatgcg
      961 attaaacttg aagaaattag cttaccaaat agtttagaaa ctattgaacg tttagccttt
     1021 tacggtaatt tagaattaaa agaacttata ttaccagata atgttaaaaa ttttggtaaa
     1081 cacgttatga acggtttacc aaaattaaaa agtttaacaa ttggtaataa tatcaactca
     1141 ttgccgtcct tcttcctaag tggcgtctta gattcattaa aggaaattca tattaagaat
     1201 aaaagtacag agttttctgt gaaaaaagat acatttgcaa ttcctgaaac tgttaagttc
     1261 tatgtaacat cagaacatat aaaagatgtt cttaaatcaa atttatctac tagtaatgat
     1321 atcattgttg aaaaagtaga taatataaaa caagaaactg atgtagctaa acctaaaaag
     1381 aattctaatc agggagtagt tggttgggtt aaagacaaag gtttatggta tta
//
LOCUS       DAVLRT010000149         1419 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLRT010000149 DAVLRT010000000
VERSION     DAVLRT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.149"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            19..1365
                     /gene="gdhA"
                     /locus_tag="VXM85_002064"
     CDS             19..1365
                     /gene="gdhA"
                     /locus_tag="VXM85_002064"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1346570.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGHLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          418 a          288 c          310 g          403 t
ORIGIN      
        1 caataaaagg agaacgatat gacatctgct aaagaatata tccaaagcgt gtttgaaact
       61 gtaaaagctc gtaacgggca cgaggctgaa ttcctccaag ctgttgaaga atttttcaac
      121 actttggaac ctgtatttga aaaacaccct gagtatatcg aagaaaatat cttggcacgt
      181 attactgagc ctgagcgcgt gatttctttc cgtgttcctt gggttgaccg tgatggaaaa
      241 gttcaagtaa accgcggcta ccgtgttcaa ttcaactcag ctgttggacc atataaaggc
      301 ggacttcgtt tccacccaac tgtaaaccaa gggattttga aattcctcgg atttgaacaa
      361 atctttaaaa acgtcttgac tggacttcct atcggtggag gtaaaggtgg atcagacttc
      421 gatcctaaag gtaaaacaga tgctgaagtg atgcgcttct gccaaagctt catgactgaa
      481 ttgcaaaaac acatcggacc atcacttgac gtacctgctg gtgatatcgg tgttggtgga
      541 cgtgaaattg gttaccttta cggtcaatac aaacgtctta accaatttga tgctggtgtc
      601 ttgactggta aacctcttgg atttggtggt agcttgattc gtccagaagc aactggttac
      661 ggtttggttt actatactga agaaatgctc aaagctaacg gtaacagctt tgctggtaag
      721 aaagtcgtta tttcaggttc tggtaacgtt gctcaatatg ctcttcaaaa agcaactgaa
      781 ctcggtgcaa ctgttatttc tgtttctgac tcaaatggtt atgtcatcga tgaaaatggt
      841 atcgacttcg atcttttggt tgatgttaaa gaaaaacgtc gtgctcgttt gactgagtat
      901 gcagctgaaa aagcaactgc aacctaccac gaaggtactg tatggactta cgctggaaac
      961 tatgacattg ctcttccatg tgccactcaa aacgaaatca acggtgaagc agctaaacgt
     1021 ttggttgctc aaggcgttat ctgtgtatct gaaggtgcca acatgccgag cgaccttgat
     1081 gccatcaaag tttacaaaga aaatggtatc ttctacggac ctgcaaaagc tgccaacgct
     1141 ggtggtgtag ccgtttcagc tcttgaaatg agccaaaaca gtcttcgcct ctcatggact
     1201 cgtgaagaag ttgatggcca tctcaaagac atcatgacaa acatcttcaa cacagctaaa
     1261 acgacttcag aaacatacgg tcttgataaa gactaccttg caggagctaa cattgctgcc
     1321 tttgaaaatg tagcaaatgc catgattgca caaggtattg tttaagattt atttactcaa
     1381 tcctcaatca ctacgattgg ggatttttag cttgaatta
//
LOCUS       DAVLRT010000150         1247 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLRT010000150 DAVLRT010000000
VERSION     DAVLRT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.150"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            215..970
                     /locus_tag="VXM85_002065"
     CDS             215..970
                     /locus_tag="VXM85_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1346571.1"
                     /translation="MIQLTASTWNINLRSRKGKQIPEMIAQELAQQNSDIICLTEYVK
                     TDNHSQFCSSLQTNGYTIFEDVRTIDFGNEILIAIKSDLVNDCQVITIDNDDNNPNFL
                     HVRVTIEGQELNIIGVRIKIGGENVFEDFKHRKTQLDKLIFNLPIHENTIILGDFNNG
                     FFKQNDDTHSYQGKPREFYSYPLLKSEMNKAGLTVHTPSEENSWKYCKLDHIIGNIPI
                     SNVCYSWRFLENPDYQNKVGFPDHAILSATISL"
     gene            1010..1228
                     /locus_tag="VXM85_002066"
     CDS             1010..1228
                     /locus_tag="VXM85_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346572.1"
                     /translation="MSDNLKAYLFLLGFVILAGIAIFIFPQGHFFSNSILILGCIPLL
                     ISTYYFAKIKLLGFYELLKELLDKIKGI"
BASE COUNT          442 a          188 c          207 g          410 t
ORIGIN      
        1 tttcttcttt taattttgta caattaaaat gtacaaaaaa atactgagag gaggaatagc
       61 aatgaacttt tttgaccaag aagccttaga ttacttctat gaggaattgg aaagctacta
      121 tgatgaagac gaactaggag aaatctttga tggattcgaa ggatagcagg aggaggtgcc
      181 tcctcctgcg gactaacttt taggaggaaa ttttatgata caacttacag catccacttg
      241 gaatataaat ctacgttcaa gaaaaggaaa acaaattcct gaaatgattg cgcaggaact
      301 agctcagcaa aattcagata ttatctgtct gacagagtat gtaaaaacag ataatcattc
      361 tcaattctgt tctagccttc aaactaatgg ttatacaatc tttgaagatg tgcgtactat
      421 agattttgga aatgaaattc ttattgcaat aaagagtgat ttagttaatg attgccaagt
      481 cattactatc gataatgatg ataataatcc aaacttttta cacgttagag tgacaattga
      541 aggacaagaa ttaaacatta ttggagttcg tatcaagata ggaggggaaa atgtttttga
      601 agatttcaaa cacagaaaaa cacaactaga caagctaatc ttcaatttac caattcatga
      661 aaacacaatt attctaggtg actttaataa tggctttttc aagcagaatg atgatactca
      721 ttcctatcaa ggtaaaccta gagaatttta ctcctatcca ttactaaaat cagaaatgaa
      781 taaagcaggt ttaactgtac atactccgtc tgaggagaac tcttggaaat attgtaaatt
      841 agaccatatt ataggaaaca ttcctatttc taatgtgtgt tattcttgga gattcttaga
      901 aaatcctgat tatcaaaata aagtcggttt ccccgaccac gctattttat cggcgactat
      961 atctttatag ttttccttaa aataattgct tattagagga ggtaatgaaa tgagtgataa
     1021 tttaaaagca tatctatttc ttttaggatt tgtgatatta gctggcatag caatttttat
     1081 attccctcaa ggacatttct tttcgaattc tatccttatc cttgggtgta ttccactact
     1141 tatctcaaca tattatttcg caaaaatcaa gctcttaggg ttttacgaac ttttaaaaga
     1201 gcttttagat aaaataaaag gtatctaact aatatgttta aattaag
//
LOCUS       DAVLRT010000151         1215 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLRT010000151 DAVLRT010000000
VERSION     DAVLRT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.151"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            57..788
                     /locus_tag="VXM85_002067"
     CDS             57..788
                     /locus_tag="VXM85_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346573.1"
                     /translation="MVNNVAVKVSNLSKEFLLGQDKTVSILKDISLSVNYGEFVSILG
                     VSGSGKSTLLSCLSSLSEPTSGEVVINGVNPYTLKEGKLAKFRRQDIAIIFQNYNLVP
                     ALPVLENVTLPLRLSGKSVDSNKVKKMLDSLNFKAELSSLVATLSGGEQQKVAITRAI
                     IADSKIIFADEPTGALDSVSRKLIFETLRNLASQGKCVLMVTHDIELASKTDRALILK
                     DGKISRQIIKPSADELYQALESSKD"
     gene            806..>1215
                     /locus_tag="VXM85_002068"
     CDS             806..>1215
                     /locus_tag="VXM85_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEV1346574.1"
                     /translation="MLKLIIYQFQYSKRQWLGTIPLLFVSSLIVGTSLFGIASSIKTA
                     NINASQLFQMLIIFGGTTLFFLISNNIRLLIDIFKKDYQLWTILGASRTQLSLLVSGQ
                     FYLMAVIVSSIGTILSFIMADSYYKFLQNLLGRD"
BASE COUNT          419 a          178 c          212 g          406 t
ORIGIN      
        1 aattccaata ttgtataatt aaatcaaaga tgacactgga atcaggagga tttattatgg
       61 ttaataatgt agctgttaaa gtctcaaacc tttcaaaaga atttttgtta gggcaagaca
      121 aaactgtctc tattttgaaa gatatttcct tatctgtcaa ttatggggaa tttgtatcaa
      181 tacttggtgt cagtggttct ggaaagtcta ctttgctaag ttgcttatca agtttatctg
      241 aaccaacaag tggcgaagtt gttatcaatg gtgtaaatcc atatacctta aaagaaggga
      301 agcttgctaa atttagaaga caagatattg caataatttt tcaaaactat aatctggtac
      361 ctgctctacc tgtactagaa aatgttacac ttcctctgag actatcagga aagagtgttg
      421 atagcaataa agtaaaaaaa atgttggata gtttgaattt taaagcagaa ctatcatcat
      481 tagttgctac cttatcaggt ggagaacagc aaaaagtggc tattactcgt gcaataatag
      541 ctgatagtaa aattattttt gctgatgagc caacaggagc tctggacagc gtctcaagaa
      601 aacttatatt tgaaacactt cgtaatttag cgagtcaagg aaaatgtgtt cttatggtca
      661 ctcatgatat tgagttggct tcaaaaactg acagagcact cattttaaaa gacggaaaaa
      721 tatctcgaca aattattaaa ccttcagctg atgagctcta ccaagcactt gaaagtagta
      781 aagattaatt tgaggaggat ttattatgct aaaattaatc atttaccaat ttcagtactc
      841 aaaaagacaa tggttaggaa caataccatt actatttgta tctagcctga ttgttggaac
      901 atctttattt ggtattgcta gttcaatcaa gactgctaat attaacgctt cacaactttt
      961 tcaaatgcta ataatttttg gcgggactac tctatttttc cttatttcaa ataatataag
     1021 actgctaata gatatcttta aaaaggatta ccaattatgg actatcttag gcgcaagcag
     1081 aactcagcta tctttattag tatctggaca gttttattta atggcagtga ttgttagtag
     1141 tattggtaca atactttcat ttatcatggc agatagttat tacaaatttt tacaaaattt
     1201 attaggaaga gatga
//
LOCUS       DAVLRT010000152         1159 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLRT010000152 DAVLRT010000000
VERSION     DAVLRT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.152"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..>1159
                     /locus_tag="VXM85_002069"
     CDS             <1..>1159
                     /locus_tag="VXM85_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1346575.1"
                     /translation="FSVIIVNCLLAVVCKKINSSSTKLLPEFSGLDFSLWQDYIQSTG
                     SIKELWPSQIELGKQGIFSGKSGIVQMPTSSGKTASINLTLRSAFYSNRIDNALIVAP
                     FRALCREIYRDINAHFVDENNVIVSEVFDLPEIPQDFSIFNDGKKRVFILTPEKLLFL
                     LRNHQSFIDEIGLCIFDEAHLFDDPSRGTNFELLLSTVKQIFPKGIQKILISAVIPNS
                     EAINRWFNEDGVIVSNNSIKTTEKRVAFSDLNGSNEQLYFIDPITFEEEFFVPRTVSV
                     SELEQLGKERKQKVFPELTNANDISIYYGTKLINNGGVGIFCGRKDTVNVILRRFIDL
                     NNRNYDLTDFLKNSDRSEVEKIGNLIGQNLGYDSVEYTCSQLGVFSHHSGIP"
BASE COUNT          415 a          136 c          214 g          394 t
ORIGIN      
        1 ttttctgtaa ttattgtaaa ttgtctctta gcagttgtat gtaaaaaaat aaatagttca
       61 tcaacgaaat tattacctga attttcagga ttagattttt ctttatggca ggattatata
      121 caatcaacag gaagcataaa ggagctttgg ccatctcaaa tcgaattagg gaagcaagga
      181 attttttcag gtaaatctgg aatcgttcag atgccaacaa gttcgggtaa aacagcctca
      241 atcaatctaa ctttaagatc agcattttat tcaaatagaa ttgataatgc attgattgtt
      301 gcacctttta gagcattatg tagagaaata tatcgagata ttaatgcaca tttcgttgat
      361 gagaataatg tgattgttag tgaagttttt gatttaccgg agattccaca ggatttctca
      421 atttttaatg atggtaaaaa aagagttttt atacttactc cagagaaatt gttgttttta
      481 ttgagaaatc atcaaagttt tattgatgaa ataggacttt gtatatttga tgaagcacat
      541 ctttttgatg atccaagtcg tggaaccaat tttgaattac tgttgtcaac ggttaaacaa
      601 attttcccaa aaggaataca gaagatttta atatctgctg taataccaaa tagtgaagct
      661 ataaatcggt ggtttaacga agatggagta attgtttcta ataattcgat taaaacaaca
      721 gagaaaagag ttgcgtttag tgatttaaac gggagtaatg agcaattata tttcatagat
      781 cctattacat ttgaagaaga attttttgtt ccacgtactg ttagtgttag tgaattagag
      841 cagcttggaa aagaacgcaa acaaaaagtt tttccagaac tgacaaacgc aaatgatata
      901 agtatttatt atgggactaa gctaattaat aatggtggag taggaatttt ttgtggaaga
      961 aaagacacgg tcaatgttat tttacgaaga tttatagact taaataatag aaattatgat
     1021 ctaactgatt tcttaaaaaa ctcagatagg tctgaagttg aaaaaatagg aaatttaatt
     1081 ggtcaaaatt taggatatga tagtgttgaa tatacatgta gtcagctagg agttttttca
     1141 catcattcag gtataccta
//
LOCUS       DAVLRT010000153         1142 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLRT010000153 DAVLRT010000000
VERSION     DAVLRT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.153"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            336..>1142
                     /locus_tag="VXM85_002070"
     CDS             336..>1142
                     /locus_tag="VXM85_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV1346576.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDKSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIK"
BASE COUNT          525 a          163 c          198 g          256 t
ORIGIN      
        1 aatcatctaa agatgaaata acataattgt cccttggtta caagggcgca attatacacc
       61 ctaaagggtg cttgcgtcct gcggagtcaa acccttgcag ggagcaacaa ccattcgctt
      121 tttaaaagtc aagggtaaat aaactcgcta aggctcgccc ttgactttta aaatttgaaa
      181 tgaaagtaac aattaagcca acatactgaa acaacaaaaa ttaattcagt agtcggcttt
      241 tttaattcta tcatttcaaa acgctcattc acaaagagga tataaaaaaa ctattaagcc
      301 tccacctaaa acaacaaaga aaggaagtga taaaaatggc agacagtttt catttttcag
      361 taaacataat atcaagagga aaaggcaaaa gtgcagtagc aagtgcagca tatataagtg
      421 gagaaaaaat aaaaaatgaa tgggacggag taacccatga ctacacaaga aaagaaaaaa
      481 tattagtaaa aaatataata ttgcctgatc atataccaaa agaatttaat gataagtcta
      541 ccttatggaa taaagtagaa atggcagaaa aaaattctaa tgcacaacta gcaagacaat
      601 tcataatagg actaccaaaa gagttatctt taagtgaaaa taaaaacctg gttgaaagat
      661 atataaaaga aaatttaaca tcacaaggaa tgatagtaga ttatgcaatt catgatgaga
      721 gtcaagataa aaatggaaat atccattgtc atataatgac cataatgcga ccaataaacg
      781 aaaaaggaga gttcttagcc aagtcaaaaa aagaatatat cctagatgaa aaaggagaaa
      841 aagtattaaa caaaaatgga aaaccaaaga caagaaaagt agaactaaca acctggaacg
      901 atacaggcaa tgtagaaaaa tggagagaaa atttttcaga tctttgtaac aaatatttag
      961 aaagagcagg agcagaaaaa agagtagacc ataggagttt taaaagacaa aattcagatt
     1021 atctaccgac aatccattta ggatcagcag caagtgcaat ggaaagaaag ggaatagaaa
     1081 ctgacaaggg aaactataat agagaaataa gaaaatataa tcaacttgta aaaacaataa
     1141 aa
//
LOCUS       DAVLRT010000154         1124 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLRT010000154 DAVLRT010000000
VERSION     DAVLRT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.154"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..249)
                     /locus_tag="VXM85_002071"
     CDS             complement(<1..249)
                     /locus_tag="VXM85_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346577.1"
                     /translation="MNNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESF"
     gene            complement(246..578)
                     /locus_tag="VXM85_002072"
     CDS             complement(246..578)
                     /locus_tag="VXM85_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1346578.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
     gene            complement(634..>1124)
                     /locus_tag="VXM85_002073"
     CDS             complement(634..>1124)
                     /locus_tag="VXM85_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346579.1"
                     /translation="FWTIMDINHLVFNKTEFLFNQNKWEKEDSFESDLTKTLKDKIQK
                     SELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCTGLVELLLYEMSI
                     SNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNFDFCMNREYHKMKSRKLLLSNKK
                     IV"
BASE COUNT          383 a          211 c          149 g          381 t
ORIGIN      
        1 aaaactttct gggaaaaact tattgattaa aatggggaaa ctcattaaca gaatcgaatt
       61 tatggctaga cagatagata ctattttatt tttcacagaa tatagaggta gtgatactat
      121 tgagtagata aaagctaaaa aaataagcaa aaaatactcc gtgtcattta taatcgccct
      181 gaatgataaa aatatataca cccaacatac gataatatgt atttttcgaa tcttatctac
      241 attattcatc ttactgtcgc agatataaat ccatcccaag gataagggtt cccgctaata
      301 tcacaacctc tattaagagc accatatcca atacagtaag ctatttttcc tgctgctgca
      361 ccaataagcc agcctccaag acctccaacc ttaatatagt ttattagttt tttggttaaa
      421 actgcagctc cagctaatgc tataaaagtt cctgaactca tagctaaagc tacgcaaatt
      481 ccttgacaag cacgcttgct taaataagct cccccatcaa gatacatcat ttcttcttca
      541 tcaatcacaa cataattatt tggtaataca agttccattt gttttctcct ccttagttga
      601 aattgtttca tttgttttac aaataggacg aacttaaact atctttttat tactcagtaa
      661 taatttccga gatttcattt tatgatactc tctattcata cagaaatcaa aattttgcct
      721 taaccaactt gtataataga aaaaatctaa ttgattgatt ctgatgaggt gtatcctaat
      781 tttatcaacc ttgttggacc agttgttatt gttactaata gacatttcat acagcagtaa
      841 ctcgacaaga cctgtacaca gaagggtgtt atgagaatct tcgatttttt tcaaaatatc
      901 ctcaaaaagt tcactatccg ttaaaaaggt ggattggttc atcgcatcat acagctgaag
      961 agacattaag tcagaataac ttagctctga tttttgtatc ttatccttta acgttttcgt
     1021 caaatcgctt tcaaacgaat cttctttttc ccatttattt tgattaaaaa gaaattccgt
     1081 cttattaaaa acgagatgat tgatatccat tatagtccag aaaa
//
LOCUS       DAVLRT010000155         1102 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLRT010000155 DAVLRT010000000
VERSION     DAVLRT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.155"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            44..1069
                     /locus_tag="VXM85_002074"
     CDS             44..1069
                     /locus_tag="VXM85_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1346580.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
BASE COUNT          433 a          150 c          185 g          334 t
ORIGIN      
        1 tattatcgat acaatagact tgaaatatgt ttaaggagtt tttatgaaaa acaaattttt
       61 tctaatagct attttagcta tgtgtatagt ttttagcgct tgttcttcta attctgttaa
      121 aaatgaagaa aatacttcta aagagcatgc gcctgataaa atagttttag atcatgcttt
      181 cggtcaaact atattagata aaaaacctga aagagttgca actattgctt ggggaaatca
      241 tgatgtagca ttagctttag gaatagttcc tgttggattt tcaaaagcaa attacggtgt
      301 aagtgctgat aaaggagttt taccatggac agaagaaaaa atcaaagaac taaatggtaa
      361 agctaaccta tttgacgatt tggatggact taactttgaa gcaatatcaa attctaaacc
      421 agatgttatc ttagcaggtt attctggtat aactaaagaa gattatgaca ctctatcaaa
      481 aattgctcct gtagcagcat acaaatctaa accttggcaa actttatgga gagatatgat
      541 taaaattgat tcaaaagcct taggtatgga aaaagaaggt gatgagttaa tcaaaaatac
      601 tgaagctcgt atatccaaag aattagaaaa acatccagaa atcaaaggaa aaatcaaagg
      661 aaaaaaagta ttatttacta tgattaatgc tgcagataca tcaaaattct ggatttatac
      721 tagcaaagat ccaagagcaa attatttaac agatttaggt ctagttttcc ctgaatcatt
      781 aaaagaattt gagagtgaag atagttttgc aaaggaaatt tctgcagaag aagcaaataa
      841 gataaatgat gctgatgtaa tcataactta tggtgatgat aaaactcttg aagctttaca
      901 aaaagatcct cttttaggta aaataaatgc aattaaaaat ggtgccgttg ctgtaattcc
      961 agataataca ccgttagcag cctcatgcac tccaacacca ctttcaataa actatactat
     1021 tgaagaatat ctaaatcttt taggaaatgc atgcaaaaat gcgaaataaa aaacaaataa
     1081 acctaggcat aatttttgta at
//
LOCUS       DAVLRT010000156         1035 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLRT010000156 DAVLRT010000000
VERSION     DAVLRT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.156"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            242..1035
                     /locus_tag="VXM85_002075"
                     /pseudo
     CDS             242..1035
                     /locus_tag="VXM85_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          362 a          179 c          205 g          289 t
ORIGIN      
        1 atgctttttg attttagtct agtataaata gcgagaaata tctatcctat cttctagaat
       61 gtcttccaaa cgaggaaact ctcgtaaaca aagaggtttt agaggcctat ttaccgtgga
      121 ctaaagttgt acaagaaaag tgcaaataag aaatctccag attaggaact atccgtgagt
      181 tctctagtct ggagattttt caatagactt cctgcgaaac aaaatatggt ataatagttc
      241 tatgaattat gaagcaagta aacaactaac cgatgcacga tttaagcgtc ttgttggtgt
      301 tcaacgcacg acttttgaag agatgttagc tgtattaaaa acagcttatc aacttaaaca
      361 cgcaaaaggt gggcgaaaac ctaaattaag cctagaagac cttcttatgg ccactcttca
      421 atatgtgcga gaatatcgaa cttatgaaga aattgcggct gatcttggta ttcacgaaag
      481 caacttaatc cgtcggagcc aatgggttga agtaactctt gttcaaaggt ggtgttgcga
      541 tttcaagaac tcctctcagt tctgaggaca cggtaatgat tgatgcgacg gaagtaaaaa
      601 tcaatcgacc taaaaaaaga attagcgaat tattctggta aaaagaaatt tcacgctatg
      661 aagactcaag cgattgtcac aagtcaaggg agaattattt ctttggatat cgctgtgaac
      721 tattgtcatg atatgaagtt gttcaaaatg agtcgcagaa atatcggaca agctggtaaa
      781 atcttggctg acagtgatta tcaagggctc atgaagatat atcctcaagc acaaattcca
      841 cgtaaatcca gcaaactcaa gccgctaaca gttgaagata aaacctataa ccatgcacta
      901 tctaaggata gaagcaaggt tgagaacatc tttgccaaag taaaaacgtt taaaatgttt
      961 tcaacaacct atcgaaatca tcgtaaacgc tttggattac gaatgaattt gagtgctggt
     1021 attatcaatc atgaa
//
LOCUS       DAVLRT010000157          976 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLRT010000157 DAVLRT010000000
VERSION     DAVLRT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.157"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(130..846)
                     /locus_tag="VXM85_002076"
     CDS             complement(130..846)
                     /locus_tag="VXM85_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1346581.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIEFISCDRDH"
BASE COUNT          288 a          152 c          194 g          342 t
ORIGIN      
        1 atttttggtt ctatttctat aaaatattcc acaaattata gaatcttcca aaaataggta
       61 agcgctacct ttttggtgtg gtataataag catagaaaaa gcccgagcga ttaggctcag
      121 gctttcttct tagtgatcac ggtcacatga gatgaattca atcttgtagt aatcagatcg
      181 tttgtaagtt tcactgtatt ctaaaacttg gccagttgat tcgagtttgg tgattttagt
      241 ttgtaggaca gtagggaatt gttcatcgac tccgaggact gaagccgcat gttctggagt
      301 tggaaagact atttcgttga tttcttcaaa gtgttcatca ttcatgtgaa tgtggtagtc
      361 taacttgaaa cgattataga tagaactata gtattcaagg tttggataat ttgcgttgat
      421 atattgttct gggatgtagg atgtatggta gatataaacg acaccgtttg attcgcggat
      481 acgttcaatc ttgtagtaga attgatcgcc gcgtagaccc aatttttcca agtaaacaag
      541 cttgtttccg cgttcaattg aaagaacagt taccttatca tctttagcat tgaagagttc
      601 aatatctgaa aactctacaa gcttgtgttt gcgtgcacgt gaaacgaagg ttccttttcc
      661 ttgttggcgg acaatatagc catctttggc aaggtcgttt aaggcgcgaa caactgtgat
      721 agagctgaca tcgtacattg aaatgagttc tgcttcagtg taaaatttat ctccactgct
      781 aaactgccca gagatgattt tattttttaa ttcgtctttt atgtattgat atttgggaat
      841 tgccatattt ttacctctat tttccttctc catagaaaat tttaccataa aagcgaaaaa
      901 aatagtagtc ttttgaataa aaaattagaa taatagtttg aaaaattaag cgatataggg
      961 attgctcata ttttat
//
LOCUS       DAVLRT010000158          964 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLRT010000158 DAVLRT010000000
VERSION     DAVLRT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.158"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            191..355
                     /locus_tag="VXM85_002077"
     CDS             191..355
                     /locus_tag="VXM85_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346582.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            352..756
                     /locus_tag="VXM85_002078"
     CDS             352..756
                     /locus_tag="VXM85_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1346583.1"
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
BASE COUNT          292 a          143 c          169 g          360 t
ORIGIN      
        1 aacaagttgg tatcaataca atataaagag gaaagtatgt ttttcatgct tgctttttta
       61 atttttacaa ttcaagaggt tttgatgacc atttatgatt tgagtgatcc aaggtcaaaa
      121 taagtgctat actagcagtg taaatgttct tgctcaacta agataaatcg cacatttagg
      181 aggaaatctt atgaagaaaa aaatactgat tattttcgtt ctgtatctaa tcatgtccat
      241 ctttctatat ccgcttaggg agagtgcttg gtatcagcta ttttatacca tagcctatgt
      301 gattgcagtt atgatctatt ttgctatcaa taaaaagaaa ggagaaaaga aatgaaaact
      361 tttcttgcta aaaaacggaa catcttcctt gcgagattgt tcttaggtca gttgcccttg
      421 cttgtttcta cttatctatt tctatctcgt cagtttttaa atttttccgt agttttccaa
      481 tttcttttag tagttattaa cttggcttct attttggtta ctgtttatct cactagggaa
      541 atgaggataa gagagtttga agatgatgat ttggttagtc ctagaaccaa tcaacttatg
      601 ttcatcggct tgacaggttt tatgtctatt atttgtttgt atagaggtat cacagcagga
      661 gaatcctacc aacaaataat cgcttatatt ggcgctgttc tctgcttaat catcatgctt
      721 ctgctcatgt ggggcttgaa gtattataaa aagtagagct taatgaagta taaaagaaaa
      781 gggtaagtgt ttttgcaata cttacccttt tataatagat tccctgcgaa acaaaatatg
      841 gtatagtata gtagattgaa actagaatag tacgcctcta tttctaaaac attgttagaa
      901 atcgatttga ctgtcctgat cgatttatcc tgttcttatt tcattttact atagttctat
      961 gaat
//
LOCUS       DAVLRT010000159          962 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLRT010000159 DAVLRT010000000
VERSION     DAVLRT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.159"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..948)
                     /locus_tag="VXM85_002079"
     CDS             complement(<1..948)
                     /locus_tag="VXM85_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346584.1"
                     /translation="MVAGQLLLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGI
                     IFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLN
                     DQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSL
                     NLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAG
                     AISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGELGGQLSSIIG
                     TKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC"
BASE COUNT          341 a          171 c          130 g          320 t
ORIGIN      
        1 gcaaatattt tttgcttcat ataacggaaa atctctattc acctcatttg gttcgatatc
       61 attcatttta tttgactcaa ttggattaat tgaatacaat tttaaaaaaa taggcttcgt
      121 accaataata gaggataatt gacctcctaa ttcacctagc gctgtaaaaa taacacctgt
      181 tagtgctcct attgcttcaa tagtaccaat tttcactatt ccttttattg caagatagcc
      241 tgttaaaaaa acgagagata tctgaaaaaa aatattgaga aagaagctaa tagcgcctgc
      301 taacgtttct acagttgtct ttctttgtat aaccatcttt aataaaattc ctgcttcttt
      361 aattttctta ggcaatacat ataaaagatt caaggacgct aacacatcaa atccattcaa
      421 tatagtctca ctagatttta aaaaagcttc attttggtta gttaaattta gactaacttc
      481 tcgcattttc gatgcaaaga tttttggtac aagtagcata atcattaatg aaaacaaggt
      541 ggctacagtc aatgaccaat gatagtgatt aagagtcaca actgcaaata tagtaccaga
      601 aattcctttt attactaaaa aaagttgttt aaaagcctga tcatttaaag tctgaacatc
      661 attatttagc cacgaaagat atgttcctga tgatttacta tgaaattctt gataggtaga
      721 gttagagatg tctgtggcaa ctctatttcg aatctctaga ttaaactctt ggatcacttc
      781 aacctgataa tttttcacta cccagtcaag gaatattatc ccacaccaga caatcatttg
      841 gtagattgac aatttcaaaa accgctctaa attcatcgca attaattcat tcaacaccag
      901 agcattaata gttgctgcat aaattagcaa taattgacca gcaacaataa atatcgttaa
      961 ta
//
LOCUS       DAVLRT010000160          957 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLRT010000160 DAVLRT010000000
VERSION     DAVLRT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.160"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(261..506)
                     /locus_tag="VXM85_002080"
     CDS             complement(261..506)
                     /locus_tag="VXM85_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346585.1"
                     /translation="MGIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSASPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
BASE COUNT          373 a          178 c          104 g          302 t
ORIGIN      
        1 aatatactta caaaaatccc cttggaatca atcaatggaa aataaaatat tgcaactaga
       61 agcgaataaa gtcccgctaa taaatatttc tgatcatctt tcattcaaaa caccatatta
      121 tttactaaga atgcatcaat acaatagagc aactctgtaa gtcaatattt gataaagcaa
      181 agctttaaac ccttccccag acgttagctt ttattcaacc cactacagtt gacaaggagc
      241 ccaaattatt gataaaaact ttagtcatca tcgtctctat aaaaatattt tctatacact
      301 cttgataata ccaaaattaa tgccaatatc ataccaagag ctataataat agaatttatt
      361 agatttattt tagtatatcc aatcactagt ccaataattg gactagcaga catgcttaga
      421 tataataaaa ataacaaaaa atgaatttca ccaattcctg caaaaaaatg tttcatagct
      481 atagttttac gcccataaat acccatcaaa tttaccgtct acgatatcta aaccatatgc
      541 aatggctcct cctaatgaga agacttcagc aacagtagcc gcaatattaa gagcattagt
      601 aatagttgcc atactaactc caccaatata tcttgttata gcacttagtt acctagataa
      661 gatatccttt agcttgcttc caaaatttaa gtctacacct ataatatgag ttcatattag
      721 tactgctaaa tcctatctat agtaatgtca aacacctaaa taaagtcatc tctccaattc
      781 attcaagaaa tgaagaaagg caaaaaatct ttaaaataag aaaaatgcat attatcaggt
      841 gttcaaaaat attgatgata tacgttatat agatagaaag atttactcaa tctcattttc
      901 aaattgagtt ttttcccagc ctcattttta atttatatct tcaatagaat ctaatga
//
LOCUS       DAVLRT010000161          946 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLRT010000161 DAVLRT010000000
VERSION     DAVLRT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.161"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            20..256
                     /locus_tag="VXM85_002081"
     CDS             20..256
                     /locus_tag="VXM85_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEV1346586.1"
                     /translation="MNNVVTTQISIQLSQELNSLLNAIVEENNTTKTDFIRQAVIEKI
                     EDMYDIKVADEAYKKWVECGKKATSHEEMMRRYG"
     gene            249..>946
                     /locus_tag="VXM85_002082"
     CDS             249..>946
                     /locus_tag="VXM85_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1346587.1"
                     /translation="MAKLYAISDIHGYLDEFRDALNKVNLNDKDNRLFLLGDYLDNGL
                     QSFQVISKIIELEEVYPNQIITLLGNHEEWFYDWLILDKPTASAFPETIKSFFSPEEL
                     NYIFKSNANNFETGVRNEIKNNIKFNPFINWFKKRYRDKRYYETDTQIFVHAGIDEEA
                     GMLWKELTSSKIFTNKFPITTGRFHKAIISGHIASWEVAKDRRYLGKIYYDSKSHYLI
                     DGDVTNSKTIPVLY"
BASE COUNT          356 a          130 c          160 g          300 t
ORIGIN      
        1 actattaggt ggctgttata tgaataatgt tgttactaca caaatttcta ttcaacttag
       61 tcaagagtta aattctttat taaatgctat tgttgaagaa aataatacaa caaagactga
      121 ctttattaga caagctgtta tagagaaaat tgaagatatg tatgatatta aggtcgctga
      181 tgaagcttat aaaaaatggg ttgaatgtgg caaaaaagct acctcacatg aagaaatgat
      241 gagacgctat ggctaaatta tacgcaatat ctgatataca tggatacttg gatgaattca
      301 gagacgctct caataaagta aatttaaacg ataaagataa tcgcctattt ttactaggag
      361 attatcttga taatggactt caatcttttc aagtcatttc caaaataatc gaacttgagg
      421 aggtttatcc taatcaaatt attactcttc tagggaatca tgaagaatgg ttttatgatt
      481 ggttgatttt agataaacct actgcttcag catttccaga aacaataaaa agcttcttct
      541 ctccagagga attgaactat atttttaaat cgaatgctaa caactttgaa acaggtgtta
      601 gaaatgaaat taaaaataat atcaaattca acccatttat aaactggttt aaaaagcgat
      661 atagggataa acgctattat gaaactgata cccaaatttt tgttcatgca ggcattgatg
      721 aagaagcagg aatgctttgg aaagaactaa cgagttcaaa gatatttaca aacaaatttc
      781 caattaccac tggtagattt cataaagcta ttatttcggg ccatattgct tcttgggaag
      841 tagctaaaga tagaagatac ctaggtaaga tttactatga tagtaaaagt cattacttaa
      901 ttgatggaga tgtaacgaat agcaaaacaa tacctgttct ttacta
//
LOCUS       DAVLRT010000162          943 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLRT010000162 DAVLRT010000000
VERSION     DAVLRT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.162"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(91..162)
                     /locus_tag="VXM85_002083"
     tRNA            complement(91..162)
                     /locus_tag="VXM85_002083"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(128..130),aa:Gln,seq:ttg)
     gene            complement(173..245)
                     /locus_tag="VXM85_002084"
     tRNA            complement(173..245)
                     /locus_tag="VXM85_002084"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:His,seq:gtg)
     gene            complement(260..330)
                     /locus_tag="VXM85_002085"
     tRNA            complement(260..330)
                     /locus_tag="VXM85_002085"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:Trp,seq:cca)
     gene            complement(335..415)
                     /locus_tag="VXM85_002086"
     tRNA            complement(335..415)
                     /locus_tag="VXM85_002086"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(379..381),aa:Tyr,seq:gta)
     gene            complement(428..500)
                     /locus_tag="VXM85_002087"
     tRNA            complement(428..500)
                     /locus_tag="VXM85_002087"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(465..467),aa:Phe,seq:gaa)
     gene            complement(504..577)
                     /locus_tag="VXM85_002088"
     tRNA            complement(504..577)
                     /locus_tag="VXM85_002088"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(541..543),aa:Met,seq:cat)
     gene            complement(588..677)
                     /locus_tag="VXM85_002089"
     tRNA            complement(588..677)
                     /locus_tag="VXM85_002089"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ser,seq:tga)
     gene            complement(761..834)
                     /locus_tag="VXM85_002090"
     tRNA            complement(761..834)
                     /locus_tag="VXM85_002090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(798..800),aa:Ile,seq:gat)
     gene            complement(869..939)
                     /locus_tag="VXM85_002091"
     tRNA            complement(869..939)
                     /locus_tag="VXM85_002091"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(905..907),aa:Gly,seq:tcc)
BASE COUNT          257 a          263 c          222 g          201 t
ORIGIN      
        1 cggcggccgg ggtcgaaccg gcacgtcctt gcggacactg gattttgagt ccagcgcgtc
       61 tgccaattcc gccacgccgg caaatagtaa ctggggtagc tggattcgaa ccaacgcatg
      121 agggagtcaa agtcccttgc cttaccgctt ggctataccc caatataaaa taggcgagtg
      181 atggggatcg aacccacgca tgccagagcc acaatctggt gtgttaacca cttcaccaca
      241 cccgccataa ttctattaac acgggcagta ggaattgaac ccacactgaa ggttttggag
      301 accttagttc tacctttaaa ctatgcccgt aaaatggaag gggagggatt cgaacccccg
      361 aacccgaagg agcggattta cagtccgccg cgtttagcct cttcgctacc cttccaaaat
      421 atataaatgg cgcgagacgg aatcgaaccg ccgacacatg gagcttcaat ccattgctct
      481 accaactgag ctaccgagcc ttattgcggg agcaggattt gaacctacga ccttcgggtt
      541 atgagcccga cgagctacct agctgctcca tcccgcgtta atataaaagg aggatgtggg
      601 attcgaaccc acgcacgctt ttacacgcct gacggttttc aagaccgttc ccttcagccg
      661 gacttgggta atcctccaaa atagttttat acgggaacaa gcccgtgtcc tcttagtgaa
      721 cagataatat tccaatcccc tagtaatgga ctaaacacta tgggcacgag tggactcgaa
      781 ccaccgacct cacgcttatc aggcgtgcgc tctaaccacc tgagctacgc gcccaagtca
      841 aaaacttggt acaaagaaca aagttcaaag cgggtgacga gaatcgaact cgcgacaaca
      901 gcttggaagg ctgtagtttt accactaaac tacacccgct aaa
//
LOCUS       DAVLRT010000163          937 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLRT010000163 DAVLRT010000000
VERSION     DAVLRT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.163"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..377)
                     /locus_tag="VXM85_002092"
     CDS             complement(<1..377)
                     /locus_tag="VXM85_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346588.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFL"
     gene            complement(692..>937)
                     /locus_tag="VXM85_002093"
     CDS             complement(692..>937)
                     /locus_tag="VXM85_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346589.1"
                     /translation="GIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVV
                     INEFIACLNSRCMPSQYDRFFYKDRPYIFSRKSPIKG"
BASE COUNT          336 a          166 c          125 g          310 t
ORIGIN      
        1 tacaagaagt agtaaaattt aaacagattc ttagttgttt gactctcctt ataaaacact
       61 gctattcctg gactaaatcc cattagttgg tgaaagagaa ttcgatagta tttcctagct
      121 cctataatga tcacaaatat aaaataaaaa taaattggtc ttaatttaca aaagattgag
      181 gaaactagta atagcaaagt atgtaagcat tgagacaaaa tatagaataa cggtatcgcc
      241 aatacaaagg ttaagatggc atgagaggga gttcctaaga tgtccaattt tataaatact
      301 gggctgacac aaaatatcgt gcttataagc agtaaaatta ttaaaattgt gtcaaaaaaa
      361 taacgcttat ttttcattta gtctactttc cattgttaaa taatactact gttaacacca
      421 agccaactta gcacattgca tcacctaatc ctacgctact tgtccataat cctttaatgt
      481 ttctactaaa attattccaa tctcccccat caagatagct tatttatcct agacaacgcc
      541 tactgcaaca gtataaataa gattcatcag ccatcttttc tggcaatcat atgggtagac
      601 taatgatatt tctagttact caaaaatatc tagctatact agagttattt ctaatcccta
      661 ggacacttgt taatccaaat gtaacaaatt ttcacccctt aataggactc tttctggaaa
      721 aaatataagg tctatcttta taaaaaaatc tatcatattg ggatggcatg catctggaat
      781 ttaaacaagc tataaattca tttataacta ctatttgttt agaggcaaca gcatcttgta
      841 taccgccctc gaaagaacca tcttgtaatt ccgaaatatt tttgtttaaa tattgttttg
      901 acaaatctct taatgcttca ttatcgtaaa tgattcc
//
LOCUS       DAVLRT010000164          910 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLRT010000164 DAVLRT010000000
VERSION     DAVLRT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.164"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            273..611
                     /locus_tag="VXM85_002094"
     CDS             273..611
                     /locus_tag="VXM85_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1346590.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            650..>910
                     /locus_tag="VXM85_002095"
     CDS             650..>910
                     /locus_tag="VXM85_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346591.1"
                     /translation="MGGAYLSKSACQGICAALAMSPGTFIALTGAAVLTKKLINYIKV
                     GGLGGWLIGAAAGVLALIFLIKICVDTTIKVLLNRIFKASKVM"
BASE COUNT          298 a          117 c          205 g          290 t
ORIGIN      
        1 aaatattttg tattctagta tgtatgatat cattgattag tctggtcata atctttccat
       61 ggggctggtc gatataatgt aagggagctt gagttgttaa aggaggctca gtccttggac
      121 aaaaatctgc tgtactacaa tgcttggaaa gatatcaaga acaaagaatg atacgattga
      181 aaataaactg attcaagaac tactgggcag tgattgaggt ttgggtgaca tcaaagaaaa
      241 gttagattaa ttctaaagaa ttgagtattt ctatgttaaa aaaatatcct tgtacgatgc
      301 aacatgatca gtcagattgt gctgcggcag ttgtttcaac agttctttta tcttacaaaa
      361 aggaactatc aatcatgaag attcgggaaa ttattggtac agacatgtat ggaacgactg
      421 tcagtggtat tgtttcaggt ctgaataagt tgaattttac agtaaaagct gttcgagtgg
      481 cactggaaga tttgactcca aaattaacat ttcctgcgat tcttcaagtt aagaatgatt
      541 taggtcaaaa tcattttgtg gtattacata gtataaagga gaaaataaat ggaactcgta
      601 ttaccaaata attatgttgc tcttgagcaa gaggagatga tgtatcttga tggggggggc
      661 ttatttaagc aagagtgctt gtcaaggaat ttgcgcagct ttagctatga gcccaggaac
      721 ttttatagca ttgactggag ctgcagtttt aacaaaaaaa ctaataaact atattaaggt
      781 tggaggcctt ggaggttggc ttattggtgc agcagcaggt gtattggctc ttatcttttt
      841 gataaaaatt tgtgtagata ctacgataaa ggttctatta aacagaatct ttaaggcaag
      901 taaggtaatg
//
LOCUS       DAVLRT010000165          884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLRT010000165 DAVLRT010000000
VERSION     DAVLRT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.165"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(63..869)
                     /locus_tag="VXM85_002096"
     CDS             complement(63..869)
                     /locus_tag="VXM85_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1346592.1"
                     /translation="MLAGSSLAISGVLMQSVTRNPIADPGILGINTGASLSVVIGLSF
                     LGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMALSSFVSFL
                     ILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNALAMGDEMA
                     VGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDIRTMTISS
                     SFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVNN"
BASE COUNT          312 a          169 c          133 g          270 t
ORIGIN      
        1 tagtgaggaa aatagtgtta ttctattttt tcttttttga atacctataa ttaaattttg
       61 cattagttat taacccctct atttttcata gttacataaa taagtactgg acccccgatt
      121 attgcagtaa ttatccctac ttcaatttca cctggtttac ctaacatacg gccgattata
      181 tcacatataa gcaagagctc tgcacctata aaagatgaag aaatggtcat tgtgcgtata
      241 tctttgctta taaataagcc acaaaagtga ggaactataa gacctacgaa gccaataggt
      301 ccaccaattg cagtaatact tgaacataaa agcacacttg caattattgc aagtgatctt
      361 atcctattaa cattaactcc aagaccaaca gccatttcat cacccatagc taaagcgttt
      421 aaatctgatg aaataaatat agctatcaag tgacctaaaa ttataaaagg tagtagtgta
      481 gatatagaag ataatgtagc tgctccaagg ctacctattt gccaaaatct aaatttgtct
      541 aagacgttat tattcggtaa aattaaaaaa cttacaaaac tgcttaaagc catactaaca
      601 caagttcctg ataaggcaag ttttataggg gtaaggcctg cttttccgct tacagcaatc
      661 gcgtatacaa aaattgcact tactaagcca ccaatgattg caaaacttat atggcttatg
      721 cttgatgaaa ttcctaaaaa agaaagacca attactacac taagacttgc tcctgtgttt
      781 ataccgagta tacctggatc agctattggg tttctagtaa ctgattgcat caatacaccg
      841 cttatggcaa gactagaacc tgctaaaatt gcaaaaatag ttct
//
LOCUS       DAVLRT010000166          858 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLRT010000166 DAVLRT010000000
VERSION     DAVLRT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.166"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            1..764
                     /locus_tag="VXM85_002097"
                     /pseudo
     CDS             1..764
                     /locus_tag="VXM85_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            757..>858
                     /locus_tag="VXM85_002098"
     CDS             757..>858
                     /locus_tag="VXM85_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1346593.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQS"
BASE COUNT          313 a           77 c          182 g          286 t
ORIGIN      
        1 ataacatgcc atggatgggt tgaatataac ttaaaaaaga aattcttgac ttattttgat
       61 ttttggacat attctatggg agatgctttt atttgtgtat ctgaaactat gaaaaaaaga
      121 ttggagagta taataaaaaa ataagaagat agttgcaata aataacggaa ttaatgtatc
      181 aaatagcgat ttagatgtag taggtgttca agactttaaa aaagaatttt gtattccaaa
      241 taacaaaaaa atcatttgtt atgttggaag gttggatcca gaaaaaaggc cggatagatt
      301 ccttgaattt gcagaaaaat tatttttagt cagagaagat gtgattttta ttatggctgg
      361 aaatggtagt atgtgggctg ctttgaaaga aaaaatttgc catttgaaat gtagagataa
      421 tttcaggtta cttggtgaaa tctatccagc tactatagtt taccaaatat cggatttatt
      481 gtatattcct tctgatacag aaggaatacc tatgtgtgtt ttagagtcaa tgtcacaagg
      541 aactccggtt ttagctagta atgttggcgg gttaagtgaa attattgaac atagggttga
      601 tggattttta tttgagaagg aagatgttga gggagtgtgt gcttgtgcta attttttact
      661 caatgattct gagtatttga aatatatagg tgagaatagt aaatcaaaaa taagaaaaca
      721 tgagagtata tgatgaatta ttagagaaga gtagtcatgg ataggaatat agatcaagaa
      781 ttagtaagta ttataatacc aacacataat aggtatgaat ctctgattag agctgtgaaa
      841 agttgtttac atcaaagt
//
LOCUS       DAVLRT010000167          827 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLRT010000167 DAVLRT010000000
VERSION     DAVLRT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.168"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(81..269)
                     /gene="pneA2"
                     /locus_tag="VXM85_002099"
     CDS             complement(81..269)
                     /gene="pneA2"
                     /locus_tag="VXM85_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEV1346594.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            complement(276..500)
                     /gene="pneA1"
                     /locus_tag="VXM85_002100"
     CDS             complement(276..500)
                     /gene="pneA1"
                     /locus_tag="VXM85_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEV1346595.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKICISAAVSGIGGLVSYNNDCLG"
BASE COUNT          270 a          162 c          102 g          293 t
ORIGIN      
        1 taaaaaaaaa acctctctca aaattattag ttacgagaga ggaaaataaa gagcaacttt
       61 tatttttaaa attgaatatt ttattttttc ttgttgtctt tgccacaacg agtctgagct
      121 gaccccaaat atgatgcaat aaaacttaat gtagcagaac atataattgt tgatctaggg
      181 tcagcaccat ctgtgccacc ataaaccaat tgcatttctt caagagataa ttctttcaaa
      241 ctctttccga ttacaaaatc atttttcatt ttaatttacc ccaaacaatc attattgtag
      301 ctaactagcc ccccaatacc agaaacagct gcactaatgc atattttcga gctagctgca
      361 gctgatttca aaataatagg agtaggcgtc caacgtggct ctgcaccatc agatgcacca
      421 taaattaaac tcatttcatc agcagacaat gattttaaac tttttccaca aaatttttcg
      481 tttgaattaa agttggtcat aagtaataac tcctccttat aaaaagttaa ataagttgct
      541 ctatattttt cgttttttca gataaattat aattctctca ttctcgtgca aaatatcggg
      601 ttttaatccg ccttcccaac atcaagacca ttggacttct tttgcgattg cactcgaatc
      661 tccaattgtt tattcttctg aataaactgt tggtaaggaa ttgtattact aaacataaaa
      721 acacctagca atgcaaatgt aactaatttt ttaattccac gtttttttct catgcaatga
      781 aacccctttc tttttccttg tttttattct aacagatata ttttatt
//
LOCUS       DAVLRT010000168          788 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLRT010000168 DAVLRT010000000
VERSION     DAVLRT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.169"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..775)
                     /locus_tag="VXM85_002101"
     CDS             complement(<1..775)
                     /locus_tag="VXM85_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346596.1"
                     /translation="MNFALNKGEIVGQVGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     VGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLRITMSGNIQSSELFKFFNENSIKVVDFETKKEMLKDIY"
BASE COUNT          266 a          148 c           87 g          287 t
ORIGIN      
        1 gataaatatc tttaagcatc tcttttttag tttcaaaatc aactacttta atagagtttt
       61 cgttaaaaaa tttaaaaagc tcactacttt gaatattccc agacatagta attctcagtc
      121 cctcttcctg aacaatatcc ccaaattctt gtttggtaat gaaaatgtct ctatctgtag
      181 ctgatgaaaa agctatctca aacaagaaat tatgactatc ttttcctact ttttgaaatg
      241 tcaaaagccc gttctccaag aaaagaaccc tctcacaaat ttcttcaata tcttctaatt
      301 tatgactcga tattaaaatt cccacatttt catgtaaagc taaatttttt agaaccgata
      361 aaactatttg tgatgattca atatctaaac cattagtcgg ttcatctaaa atcaatatat
      421 caggttccgt aacgagagtt agaagcaaag ctaatttttg ttttgtaccc aaagaatagg
      481 tctttacttt tttattaata gactgagtca aatctaactc ttggatcaaa cttctaaatc
      541 tttcttgatt gtagtcaaca ccatataaat ttgacaaata ttttaaattc tctaaacctg
      601 ttttagataa aaataatttt ggttcttcga ttaaataccc aacattatca ctgcttataa
      661 tattacctga agtcggttga ttattctgaa caagaatttt catcaacgta ctcttaccaa
      721 ctccatttct ccctacttga ccaacaattt cacccttgtt aagtgcaaaa tttatgtctt
      781 tgagaatg
//
LOCUS       DAVLRT010000169          785 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLRT010000169 DAVLRT010000000
VERSION     DAVLRT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.170"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            168..>785
                     /locus_tag="VXM85_002102"
     CDS             168..>785
                     /locus_tag="VXM85_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1346597.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFQYVSDSSMVGYMGKTKELKMYQEELDEKNYIDRI"
BASE COUNT          279 a           83 c          153 g          270 t
ORIGIN      
        1 aatattgagg agaatttaga ggatgatatt gaaaggatat tgaaatattt atatatatgt
       61 ttatttaacg aaatattcat tattaagaat aaggtgaatt tttttgatga tgtggaattt
      121 aatcagacac taagtgagtt tttagataaa ttgtagaggg aatggttatg agtaacaata
      181 ctaagtatat ctttttatct ccacatttag atgacgcaat tttttcctgt ggtgattata
      241 tttcaaaatt aacttcagaa ggagaaattg tacttgttat tactatattt tctggttacc
      301 ctttgagcca acagctacag ccctctgcta agcaatttca taagttgtgt aaccttggga
      361 aatatccgat tgaagaaagg aagaaagaag acaggttagc ttgtgaacgt ctacaatgtg
      421 attttagaca cttatcgtac tatgaatgtc tctataggaa agatagaaat ggcaattttc
      481 tataccgtca tatctatagt gaattaaaaa atgaggacac attaaagaat gatatcataa
      541 aagagctgtt aatgcattta gacgacaaat gtgtcgtata ttgtccgctt tcacttggtg
      601 accatgttga tcatgttttt gtgaatagta ttggaagggc gcttgaattt atgcgttata
      661 aagtaatata ttatgaggac ttccaatatg taagtgatag tagtatggtt ggttatatgg
      721 gaaaaactaa agaattaaaa atgtatcaag aggaacttga tgaaaaaaat tatattgata
      781 gaata
//
LOCUS       DAVLRT010000170          770 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLRT010000170 DAVLRT010000000
VERSION     DAVLRT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.171"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..574
                     /locus_tag="VXM85_002103"
     CDS             <1..574
                     /locus_tag="VXM85_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1346598.1"
                     /translation="KIPSGLYKIDTGKVLFDGININQIDKKILSQNLGVVPQDSFLLN
                     RSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIA
                     LARALINNPSIVILDEATSALDTINEERITKYIKSQGCTQIIVAHRLSTIKDADIIFV
                     MKGGKIVESGNHKYLMELGGEYYSLYTKRK"
     gene            587..757
                     /locus_tag="VXM85_002104"
     CDS             587..757
                     /locus_tag="VXM85_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346599.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
BASE COUNT          294 a           80 c          153 g          243 t
ORIGIN      
        1 aaaaatacca tcaggattat ataagattga tacaggaaaa gttttatttg atggtataaa
       61 tattaatcaa atagataaaa aaatattaag tcaaaattta ggagtagttc cacaggattc
      121 atttttattg aaccgaagta ttcttgataa tataacttta aagaacgaag ttacttcaca
      181 aaagatagag gaagtttgta aagcagttca aatctatgat gaaatcatgg ctatgccgat
      241 gaaatttaat actatcatct cagagatggg ttcaaatatt tcaggtggtc aaagacaacg
      301 gatagcactg gcacgtgcat taataaataa tcctagtatt gtaattttag atgaagcaac
      361 tagtgcttta gacactatta atgaggaaag aataacaaag tatataaaaa gtcagggctg
      421 tactcaaata attgtagctc atagattgtc aacgattaag gatgcggata ttatttttgt
      481 aatgaaaggt ggtaagattg ttgagtcagg aaatcataag tacttaatgg agcttggtgg
      541 agagtactac agcttatata caaaaaggaa atgaggtgta aagaaaatga agaaagaaaa
      601 tgaatatgta attttaacaa cagcctcact aggggtgatg attggaatag tgtttgcaat
      661 ttttttagat tttccagttg aatatggtat ttctttaggc ttgttgaatg gaatagtatt
      721 gggttcgctg attgtttaca aaaacaataa gaattaagca taattttttg
//
LOCUS       DAVLRT010000171          763 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLRT010000171 DAVLRT010000000
VERSION     DAVLRT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.172"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            1..763
                     /locus_tag="VXM85_002105"
                     /pseudo
     CDS             1..763
                     /locus_tag="VXM85_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
BASE COUNT          284 a           89 c          139 g          251 t
ORIGIN      
        1 gacagattat gatgagaaaa ttggtagttt taatacatat gaaatcgaac aaacggtatc
       61 gaagagtctg agggtaaaac ctaatggaaa gattatttga aagtcaacag ttccgatcgg
      121 cttttcaaac aaattaaaaa ggctgtttga tacaaaaaat atcatttttg tccctgaatt
      181 tttgagagaa ggttgttcta tatatgataa tttatatcca agtcgcatag ttgttggaga
      241 tgagacagtt gaaggaagaa aaattgcaga gttgttcctt tcgattagta ctcatagtac
      301 tgccaatatt aaaaatgtta tgttagtttc tcctactgaa gcagaagcaa ttaagctttt
      361 ttctaacaca ttcttagctc tccgtgttgc tttttttaat gaactagatt cttttgctga
      421 gaggagaagt ttaaatgctg aagttgtaat aaaaggtgtt tgtttagatc caagaattgg
      481 aaatttttat aataatcctt cttttggatt tggaggatat tgtcttccca aagatactaa
      541 acaattaaaa aaagaattta tagaaataaa tgccccagtg atagaagcga ttgatatttc
      601 aaacacaaat agaaaacagt tcatagttaa acaaatattg gaacgaaagc caaagatagt
      661 aggaatatat aaattgggga tgaaatataa ttcagataat tacaaagagt cagctatttt
      721 aagtataatt aatgaactat tgattgtcgg tataaaaatt tta
//
LOCUS       DAVLRT010000172          701 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLRT010000172 DAVLRT010000000
VERSION     DAVLRT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.174"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            222..698
                     /locus_tag="VXM85_002106"
     CDS             222..698
                     /locus_tag="VXM85_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1346600.1"
                     /translation="MNVDGNKFYNVSVSEDIVNAGQILEDYFYVDKFGNINLKGTPEE
                     LAKNIGISVQEASLMYEAVKELPNVYERGPVGFRFNLGPQVRGMGGWAAGAFATGYAG
                     WHLKQFAVNPVTSGFVAVISGAIGWAVKTAVENYWTVAVATVEVPFVNLVYTIDLP"
BASE COUNT          213 a           90 c          162 g          236 t
ORIGIN      
        1 ggaaacaata ttgccagaat aaactatatt tagatgcaca tttcattcat tgttttataa
       61 aaggagaaga taaacggcta ctaaaaagag ttttaaagcg ttagttgtag gactaggtat
      121 tgtttcaata ttctcatcag ccttacctat ggttagtggt tctgtatttg cagatagtgc
      181 cctaactaca gtatacagta gataaagcaa atgatattgt tttgaatgtt gatgggaata
      241 aattttataa tgtttcggtt tcagaagata ttgtaaatgc tggtcaaatt ttggaagatt
      301 atttttatgt agataaattt ggaaatataa atttaaaagg cactcctgaa gagttagcaa
      361 aaaatattgg tatttctgta caagaagcaa gtttgatgta tgaagctgta aaagagttac
      421 ccaacgttta cgaaagaggt cctgtaggtt ttcgtttcaa tcttggtcct caagtgaggg
      481 ggatgggtgg ctgggctgct ggagctttcg ctactggata tgctggatgg catttgaaac
      541 aatttgcggt taatcctgtt acatctggat ttgttgctgt aataagtggt gcgattggct
      601 gggctgtaaa aactgctgta gaaaattatt ggacagttgc tgtagctaca gtagaagtgc
      661 cgtttgtgaa ccttgtttac accatagatt taccttagag g
//
LOCUS       DAVLRT010000173          662 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLRT010000173 DAVLRT010000000
VERSION     DAVLRT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.175"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            77..439
                     /locus_tag="VXM85_002107"
     CDS             77..439
                     /locus_tag="VXM85_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1346601.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT          220 a           81 c          121 g          240 t
ORIGIN      
        1 aacttcaaaa cagtgttttg agcaacctgc ggctagtttc ctagtttgct ctttggtttt
       61 cattgagtat aacagattgt tagaagttgg tttaaatttc ctaatcagtt tgttcacatt
      121 taccttcgat atattatatc ccatagttaa ggttggtcat acagatgatt atagtcatgg
      181 agccgtaaaa cttagtgttt ctttagttga caaagatgcc atgaaaaaaa tatctgtaac
      241 tgtaatagga tattttgaaa taaatataga tgaaaatatc accgatattc tatacgtaaa
      301 tggtactgct attctttatc attatttacg ttctattgtt tcaatagttt cggcaattga
      361 tagcagtgaa gcaatgttgc tacctaccat taatgtttta gagttactag ataaatctca
      421 accttttgaa gaagaataat ttattagctc actaaattga gggtaaggaa aagtaaaatc
      481 agtaagaaaa atgtcttgca ttatacagca accttttggg aatgagtaga tggattgaat
      541 aaaattttat taagagtgga tgatttatct gtagattatt attggacagt tagtcttgaa
      601 gtagtctaag aattaggtta taatcagtag aatccttgct aataatgagg aggttagttt
      661 at
//
LOCUS       DAVLRT010000174          617 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLRT010000174 DAVLRT010000000
VERSION     DAVLRT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.176"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(1..617)
                     /locus_tag="VXM85_002108"
                     /pseudo
     CDS             complement(1..617)
                     /locus_tag="VXM85_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          184 a          128 c          114 g          191 t
ORIGIN      
        1 tccaaaattc gaaaggcttt attcttaaat ccacgtttac gaatctcttt ccacacttgt
       61 tcaatggggt tcatctctgg tgtgtatgga ggaataaatg caaaaccaat attagtcgga
      121 atctttaaga tacttgattt atgccatata gcattgtcca taacgagtaa aaaataatca
      181 tctggataag cttgtgaaag ctcttctaaa aagacgttca tccactcagt attacatcca
      241 ccagctctta agaaaaatga ttcgcctgta taggcatcaa cagctccata acaatagcga
      301 aattctcgta tatagtgact atggacatgt ggacctactc ctattggaga ccaacaagat
      361 cccagtttac tgattctacc gaaaccagcc tcatcttggt acatcaagcg aaccttatga
      421 aaaggtctac tggttttaaa gcgctttctt gtcttcttga attgagattt tatttttaga
      481 cgcgacaatg gtttgagcgt ctgctttctt aggatgttct ggacgtggcg taatatttcg
      541 ccaaccatgg cgcttcaaca gttgatagaa ggcatcacgt gtgtaggaac gacctaactc
      601 ctttttataa gcctgaa
//
LOCUS       DAVLRT010000175          601 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLRT010000175 DAVLRT010000000
VERSION     DAVLRT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.177"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            282..601
                     /locus_tag="VXM85_002109"
                     /pseudo
     CDS             282..601
                     /locus_tag="VXM85_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          200 a          114 c          161 g          126 t
ORIGIN      
        1 caccagcacc agaagacgga gaaaatgagc ttttagtcgc taaaaccgag ttcaagagga
       61 cagctgatgg cctatctact aagatggcag cagttgagag ctatgttggt caagacggtc
      121 agcgacagga agccctacag cgctatactc gtgaggaaag cgcgagacaa gcgacagcag
      181 tccgtgagct ggtcaatcgt gatttcgttg gtaaggctac ttatcaagaa gatgttaagg
      241 gtatcaatca gaggattgaa gctgttaaaa ctagtgcgaa tactgaaggc cttagaacca
      301 aagcggatat cagcgcactc aatgtgactg ctgaaaatat ccggcaatct gtgaagagcc
      361 ttgagacaga cacgcagaac aagctaaatc agaagttgag tcaggctgaa tttgaggtgc
      421 gagctggctc tatccgtcag gaaatcctga acgcaaccaa ggataaagca gataagactt
      481 tagttgtatc tgaagccggg aaattgcgtg aagaattttc aaaaatgaag gtgggaggcc
      541 ggaatctatg gataaaatcc aagacggttg gagctgtaat tgaaaaatta cctgaaaacc
      601 a
//
LOCUS       DAVLRT010000176          594 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLRT010000176 DAVLRT010000000
VERSION     DAVLRT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.178"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            complement(<1..143)
                     /locus_tag="VXM85_002110"
     CDS             complement(<1..143)
                     /locus_tag="VXM85_002110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComC/BlpC family peptide pheromone/bacteriocin"
                     /protein_id="HEV1346602.1"
                     /translation="MQLGDFAKAGVGGAAARGLQLGIKTGTWQGAATGAAGGAILGGV
                     AYAA"
     gene            complement(80..214)
                     /locus_tag="VXM85_002111"
     CDS             complement(80..214)
                     /locus_tag="VXM85_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEV1346603.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWEILPKQVLEEQQQEVFN
                     "
     gene            complement(283..552)
                     /gene="blpJ"
                     /locus_tag="VXM85_002112"
     CDS             complement(283..552)
                     /gene="blpJ"
                     /locus_tag="VXM85_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV1346604.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGSGLTCLGGMVGDKLGLSW"
BASE COUNT          172 a          168 c           76 g          178 t
ORIGIN      
        1 gctgcatagg ctacacctcc aagtatagct ccacccgcag caccagttgc tgcaccttgc
       61 catgttcctg ttttaatgcc tagttgaaga cctcttgctg ctgctcctcc aacacctgct
      121 ttggcaaaat ctcccaattg catccgccac cttcaacgca agcaagcatt tcagtatcca
      181 taacagaaaa ttgtgacatc atttttgtat ccatgacaaa tactcctttt ttaaaaaact
      241 aaaataaatc agaatagaat cctcataatt ttactataag tcttaccaac ttagtcccaa
      301 tttatcacca accatacctc ctaagcatgt taatccactc ccaattgcac caatgtgtgc
      361 tccaacaaat gcaccagcaa gtccagctac tcctaaagtg gccaaacctg ctccagttcc
      421 accagttata attcccgtag tgactcctgt aatcagtgca ttttgacaat cagtggagct
      481 atacccccct tcaactttcg caagcatttc agtatccata acctctaact gtgacaacat
      541 ttttgtattc atgatgaata cctccttttt attttcaatt tgttaccaaa gtct
//
LOCUS       DAVLRT010000177          581 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLRT010000177 DAVLRT010000000
VERSION     DAVLRT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.179"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..>581
                     /locus_tag="VXM85_002113"
     CDS             <1..>581
                     /locus_tag="VXM85_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1346605.1"
                     /translation="IKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVAD
                     LVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALAL
                     AQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFA
                     IKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIR"
BASE COUNT          222 a           99 c           98 g          162 t
ORIGIN      
        1 tataaagcca ttagagggag aagtattgct tgataataaa tcaattaatt cttataaaga
       61 aaaagattta gcaaaacaca tagctatatt acctcaatct ccaataatcc ctgaatcaat
      121 aacagtagct gatcttgtaa gccgtggtcg tttcccctac agaaagcctt ttaagagtct
      181 tggaaaagat gaccttgaaa taataaacag atcaatggtt aaggccaatg ttgaagatct
      241 agcaaataac ctagttgaag aactttctgg gggtcaaagg caaagagtat ggatagctct
      301 agccctagcc caagatacaa gtatcctact tttagatgag ccaactactt acttggatat
      361 ctcatatcaa atagaactat tagacctctt gactgatcta aaccaaaaat ataagacaac
      421 catttgcatg attttgcacg atataaatct aacagcaaga tacgctgatt acctatttgc
      481 aattaaagaa ggtaaacttg ttgcagaggg aaagcctgaa gatatactaa atgataaact
      541 agttaaagat atctttaatc ttgaagcaaa aattatacgt g
//
LOCUS       DAVLRT010000178          499 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLRT010000178 DAVLRT010000000
VERSION     DAVLRT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.180"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            <1..>499
                     /locus_tag="VXM85_002114"
     CDS             <1..>499
                     /locus_tag="VXM85_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1346606.1"
                     /translation="EIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIK
                     KDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMP
                     YNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPN
                     ERPHS"
BASE COUNT          161 a          104 c          113 g          121 t
ORIGIN      
        1 atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt tatatctttg
       61 atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat atgacccata
      121 gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc caggcttatg
      181 ctaaagagaa aggtttgacc cctccttcga cagaccatca ggattcagga aatactgagg
      241 caaaaggagc agaagctatc tacaaccgcg tgaaagcagc taagaaggtg ccacttgatc
      301 gtatgcctta caatcttcaa tatactgtag aagtcaaaaa cggtagttta atcatacctc
      361 attatgacca ttaccataac atcaaatttg agtggtttga cgaaggcctt tatgaggcac
      421 ctaaggggta tactcttgag gatcttttgg cgactgtcaa gtactatgtc gaacatccaa
      481 acgaacgtcc gcattcaga
//
LOCUS       DAVLRT010000179          482 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00040330
            SAMN33607155-rid17949693.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLRT010000179 DAVLRT010000000
VERSION     DAVLRT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607155
            Sequence Read Archive: SRR23725692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721569.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721569.1
            Genome Coverage       :: 198x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:24:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,115
            CDSs (total)                      :: 2,069
            Genes (coding)                    :: 1,946
            CDSs (with protein)               :: 1,946
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 60 of 123
            Pseudo Genes (internal stop)      :: 35 of 123
            Pseudo Genes (multiple problems)  :: 28 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607155-rid17949693.denovo.181"
                     /strain="AUSMDU00040330"
                     /serotype="22F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST5860"
     gene            31..>482
                     /locus_tag="VXM85_002115"
     CDS             31..>482
                     /locus_tag="VXM85_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1346607.1"
                     /translation="MGIKNSRGKYISFLDDDDEYMPDRILKLMACFKKSRMKNLALVY
                     SYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQ
                     DGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEY"
BASE COUNT          178 a           54 c          101 g          149 t
ORIGIN      
        1 gaagatttag gtgcaactaa tgccagaaac ataggtatca aaaattctag aggtaagtat
       61 atatcatttt tagacgatga tgatgaatat atgccagatc gaattttaaa gttgatggct
      121 tgttttaaaa agagtagaat gaagaattta gctttagttt atagttatgg aataataatt
      181 tatccaaatg gtacacgaga agaggagaag accgattttg ttggaaatcc cttgtttgtt
      241 caaatggttc acaatatagc aggtacgtca ttttggttgt gtaaaaaaga ggtgctagaa
      301 ttaattaatg gttttgagaa aatagattca catcaggacg gtgttgtttt attaaaacta
      361 cttgctcaag gataccaaat tgatatagtg cgagaattct tggtgaatta ctacgctcac
      421 agtaaagaaa acggtatcac tggagtgaca caaaaaacaa ttaatgcaga tgaagaatat
      481 ta
//
